LOCUS       DAOOQX010000001         9819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_8558_length_9819_cov_3.128329, whole genome shotgun sequence.
ACCESSION   DAOOQX010000001 DAOOQX010000000
VERSION     DAOOQX010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8558_length_9819_cov_3.128329"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1043
                     /locus_tag="PLX53_00005"
     CDS             159..1043
                     /locus_tag="PLX53_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71492.1"
                     /translation="MLYLPLCILSSAVILVVFKIAERMKINTLSIILINYIIACLAGF
                     FLFRGEGAFSGQFSPGSIAFMVCLGVLLVVVFRLVGLSTQRAGIAATSVASKMSVVLP
                     ILFSIWIDPHDKLTSTKLAGIVVALVAVILSVYRNDNPRDSLRKIYLPVLIFFGIGTI
                     DSSVKYAQATFVTGELNPLFNFVIFGVAFIAGFAMVAFSKPALKDLQHLKTWLVGVVL
                     GLANFGSMYFMIAALNHINPATGKPAQGSVVFGINNIGVVVLGVLLGYTFFKERPTRL
                     NWSGIALSVVAIFILMNS"
     gene            1043..1654
                     /locus_tag="PLX53_00010"
     CDS             1043..1654
                     /locus_tag="PLX53_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOY71493.1"
                     /translation="MQADDTYQTIELKAEGLYKEKGSRFIALAFPVSSEVQVKAILDD
                     LRQEYHDARHHSFAYIVGYKGERWRTNDDGEPSGTAGKPIYGQLLSFNLTNVLVVVVR
                     YFGGTKLGVSGLINAYKTAACNALNNARIVTKTIDQTFSVFVDYPLVGEVMRVIKDLS
                     IQVIEQQFDTRCMVRVSVRQSLCGEFTSRINKVESAWIDSVDQ"
     gene            1718..2626
                     /gene="pyrB"
                     /locus_tag="PLX53_00015"
     CDS             1718..2626
                     /gene="pyrB"
                     /locus_tag="PLX53_00015"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOY71494.1"
                     /translation="MKSKSLVSINDLSRDEMLRVLDLAEEFERNPSQPILQGKVVATL
                     FFEPSTRTRLSFESAVNRLSGRVIGFSEASSSSVSKGESLKDTILTVANYSDLIVMRH
                     PVEGSARYASEVSPVPIINAGDGANQHPTQTLLDLYSIRKTQGTLNDLKIVMVGDLKY
                     GRTVHSLLMAISQFNASFVFIAPPELQMPQEYKLFLKESGLKFEEHTDLADNIVDADI
                     VYMTRVQRERFSDPMEYEKVKNVYVLRNHMLANTKPNLKVMHPLPRVNEISIDVDDSS
                     KAYYFTQALNGVYTRMAIMSLILGLK"
     gene            2712..3170
                     /gene="pyrI"
                     /locus_tag="PLX53_00020"
     CDS             2712..3170
                     /gene="pyrI"
                     /locus_tag="PLX53_00020"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOY71495.1"
                     /translation="MKDKKHLSVSAIENGTVIDHIPAQNLFKVISILGLDRIPNQITF
                     GTNLESQRLGRKAIVKIADKYFEREEINRIAIVAPHAKLNIIRDYEVVEKMLVEVPDH
                     ITGIVRCVNPKCITNFENVAPKFEVVDKKNVSLKCHYCEKITDQEHMEYR"
     gene            complement(3313..4647)
                     /locus_tag="PLX53_00025"
     CDS             complement(3313..4647)
                     /locus_tag="PLX53_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HOY71496.1"
                     /translation="MKPQSVISKFFHYFNLESELDSYDRIHESIVKDVVFRGTNLWIL
                     MFAIIIASVGLNMNSTAVIIGAMLISPLMGPINGMGYSIATYNMKLFKISLKNYGFAV
                     LAGLFASTLYFSISPISTAHSELLSRTTPTIYDVIIALFGGLAGIVAISSKQKGNVIP
                     GVAIATALMPPLCTAGYGLATGQFNFFFGAFYLFTINTVFIGISSVAISQILKFPIIA
                     DMDVRQKKSINRWISTVIIVVLLPSIYFGYRLVQNEKFNLNASRYISNVSSFEGNYLL
                     RNNIDVRKKIITLTYGGVSLTEKQKEAITQKASNFDLDDAKILIEQGLALNSSNETDS
                     EVIKLRSELTRLNMIIRDKDAQIDSISRKRQLGKTLLLEIQAFFPKISSCSYAETFQY
                     KTDSSQPDKTTVVFFGITSGITPAEKKQISNWLNARLNQDDIKVFWEYVPKN"
     gene            5175..6296
                     /locus_tag="PLX53_00030"
     CDS             5175..6296
                     /locus_tag="PLX53_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOY71497.1"
                     /translation="MTAFSKRLIRISTTVAIALLLVGIILYPKVKDASNSDSKDKVPG
                     RMAMRGGGGQPLFANGFIIVPTSMSELISTTGSLLPDEEVDLSFETSGKVVAIYFEEG
                     TKVKKGDLLAKINDAPLQAQLQKLQAQRKLAEEKLFRQKQLLERDAISRESYDQVSTE
                     LQAIEADIMLIKARIAETELRAPFDGIVGLRLISEGAYTSTQTPIAHLVKVSPLKIEF
                     SIPERYAGEVTPGYDISFVIDGIESKFTGKVYAVAPKVDMATRAIVVRAMYPNPNEEL
                     KPGRYASITLQLSKIENAVSIPTEALIPEMEGEKVFIYRNGKAEQVRVTTGLRTESRI
                     QVRTGLNFGDTLLTSAILQLRQGIPVQLDTIITNAINLQ"
     gene            6302..9370
                     /locus_tag="PLX53_00035"
     CDS             6302..9370
                     /locus_tag="PLX53_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOY71498.1"
                     /translation="MSLSSLSINRPVLSTVFSLIILLLGAIGITFLGVREFPSVDPPI
                     ISVSTSYPGANSDVIETQITEPLEQSINGIPGIRTLTSRSSQGSSRITVEFELSVDME
                     TAANDVRDKVSQAQRLLPRDCDPPTVSKADADARPIMNIAINSKKRSLLELSEIADLT
                     LKEQLQTISGVSSVGIWGEKRYAMRIWLDPAKLAGYQMTPLDVRNALNRENVELPAGS
                     IEGANTELTIRVLGLMSTPEEFNNLILKRSGEQLIRVRDIGRAELAPEDLRSIMKMNG
                     VPMVSVVVIPQPGANQIEIVDEVYKRIDFIKKDLPDDVNIVVGFDNTQYIRSSIKEVQ
                     TTIYIAFFLVVVIIFAFLRDWRTTLIPILVIPVSLVGVFFIMYLAGFSINILTLLAIV
                     LSIGLVVDDAIIMMENIYVKIEQGMTPLEAGVKGSNEIFFAIIATTITLISVFFPIVF
                     LEGMTGRLFREFSIVIAGAVAISAFVALTFTPMLSTKLLKTRHTRSKAYSYTEKFFTS
                     LNSGYRNSLSSFLKHRYLPVVILVVAAGLIVLLWKSIPAEMAPMEDRSQVSLSVTFPE
                     GTTYDYSAAYVEDVAALVDKLVPEAEKATSRAWRGGASMDVVLIPPSERDRSQQDIAN
                     MLTAELRKKTKARVNVIQQSTFGGRRASLPIQYVLQAQNIDKLREILPAFMAEVQDNP
                     TFTMADVNLRFTKPELQVEINRDKANMLGVSTQNIGQTLQLALSGQRFGYFIMNGKQY
                     QILGELPRATRNEPLDLKSLYVRSDAGQMVQLDNFVEVKESTAPPQLYRYNRFVSATI
                     SAGLAEGKTISQGIEEMDKIAAKTLDDTFRTALAGDSKDFTESSSSLMFAFAMAIVLI
                     FLVLAAQFESFKDPVIVMMTVPLALAGALIFMWYFNITMNIFSQIGIIMLIGLVSKNG
                     ILIVEFSNQRKNAGMSKLDAVKYAAAARFRPILMTSLSTILGILPLALGLGEGAQSRV
                     AMGTAVVGGLTVATFLTLYVIPGVYLLISSESAAGKAANSKGREVEGIEQ"
     gene            9482..>9819
                     /locus_tag="PLX53_00040"
     CDS             9482..>9819
                     /locus_tag="PLX53_00040"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOY71499.1"
                     /translation="MNGKFDLMNQTTPSALAAPMAKRIFTITLLICLCSGLSKSQELF
                     DLSSCIKAGLEQNFSILIARNQEKIASNNYTVGNAGYLPTLSGSGRYGGTSNTTHQSF
                     TDGTESEANGV"
BASE COUNT         2603 a         2186 c         2327 g         2703 t
ORIGIN      
        1 gtcctctaaa aagtagcatt tattggttga atttaggtgc cgtatgtaga tagttgttaa
       61 atagaaatgt agcatgtttt agtcatctaa aggatcatgt ttataattta attttggcta
      121 cttccaaaat tcttgtaatt ttgccaaaac ctgatgcgat gttataccta cctctctgca
      181 tcctttcctc ggctgtaata ctggttgttt tcaagattgc tgaaaggatg aaaatcaaca
      241 ccctcagcat cattctcatc aactacatca ttgcctgcct agcaggtttt tttttgttca
      301 ggggtgaggg tgccttttct gggcaattta gccctggcag tattgccttc atggtttgtc
      361 tgggagtact tctcgtggtg gtttttagat tggtgggctt aagcacccag cgtgcgggaa
      421 ttgcagctac ctcggttgcc tccaagatgt cggtcgttct ccccatcctg ttctctatct
      481 ggattgatcc acacgataag cttacctcca caaagcttgc cggcatagtg gttgcgttgg
      541 tagcagttat cctttctgtt taccgaaacg ataaccccag ggattcactt cggaaaatat
      601 accttcctgt cctcattttt tttgggatag gaactattga ctcgtcggtg aagtatgccc
      661 aggccacctt tgttaccggc gagctgaacc cgcttttcaa ctttgtaatt tttggagttg
      721 cgtttattgc cggtttcgca atggtggctt tttcgaagcc agcgctgaaa gatttgcagc
      781 accttaaaac gtggctagtc ggagtagtac tgggactagc caactttggc tcgatgtact
      841 tcatgattgc tgctctcaac cacatcaacc cggctacagg caagcctgcc cagggttcgg
      901 ttgtgtttgg aattaacaat ataggcgtgg tggttctcgg agtgcttctg ggatatacat
      961 tttttaagga gcgcccaacc cgtctcaact ggtcgggaat cgccctgtcg gttgttgcaa
     1021 tttttattct aatgaatagc tgatgcaggc tgacgatact taccaaacta ttgagctgaa
     1081 agccgaaggc ctttacaagg aaaagggtag ccggtttatt gcgctggctt ttcccgtatc
     1141 ttctgaggtg caggtaaagg ctattctcga tgaccttagg caggagtacc atgatgcccg
     1201 ccatcacagc tttgcataca tagttggcta taaaggtgag aggtggagaa ctaacgacga
     1261 tggtgaaccc tccgggactg ctggcaagcc aatatacgga cagctcctca gttttaacct
     1321 gaccaacgta ctggtggtgg tggtacgata ctttggcggg acaaagcttg gcgtttcggg
     1381 tctgattaac gcatataaaa ctgccgcctg caacgccctc aacaatgccc ggattgtaac
     1441 caaaacaata gaccaaacat tttcagtttt tgttgactac cccttggtgg gtgaggtgat
     1501 gagggttatt aaggatttaa gtattcaggt gattgagcag cagttcgata cccggtgcat
     1561 ggtgcgcgta tcggtgcgcc aaagcctttg cggggagttt acctccagaa tcaacaaggt
     1621 ggaatcggca tggatagaca gcgttgacca gtaattgttt gtaaagccct gttaaccggg
     1681 gctttttttc tacatataac ctaaaaacaa catgagcatg aaaagtaaaa gtttagtttc
     1741 aattaacgac ctgagtaggg atgaaatgct cagggtactt gacctggcgg aggaattcga
     1801 gagaaaccct agccaaccaa ttctacaggg caaagtggtt gctacacttt tctttgagcc
     1861 atctacccgt acccgtctga gcttcgagag cgcagttaac aggttgagcg gcagggttat
     1921 tgggttttcc gaggcctcgt cgagtagcgt ttcaaagggt gaaagcctca aggacaccat
     1981 tctaacagta gccaactact ccgacctgat tgtaatgcgg catccggtgg agggtagcgc
     2041 ccgttatgcc agcgaggtat cgccggtgcc gattattaat gcaggcgatg gcgccaacca
     2101 gcaccccacc caaacgctgc ttgacctgta ctccatccgc aaaacacagg gaaccctcaa
     2161 cgacctaaag attgtgatgg tgggtgacct taagtatggc cgaactgttc actcactact
     2221 gatggccatt tcgcagttta acgcctcctt tgttttcatt gcccctcccg agctgcaaat
     2281 gcctcaggag tacaagctgt ttcttaagga atcgggcctg aagtttgagg agcacaccga
     2341 cctggccgat aatattgttg atgccgacat tgtgtatatg acccgtgtgc agcgcgagcg
     2401 tttttcggac cccatggagt acgaaaaggt aaaaaacgtt tacgtgcttc gcaaccacat
     2461 gctagccaac accaagccca acctgaaggt gatgcatccg ctgccgcggg taaacgaaat
     2521 tagcattgat gtggacgaca gctccaaagc ctactacttt acccaggccc taaacggggt
     2581 gtacacccga atggccatca tgtcgctaat tcttggactg aagtagctac gaggtgccat
     2641 gacggaatgc atggcgtggt agtgtatatt ttataattcg ttgcaattta accagctaat
     2701 aaattaattc gatgaaagac aaaaaacatt taagtgtaag tgcaattgaa aacggaacgg
     2761 ttattgacca cattcccgct cagaacctat ttaaggttat ctcaattctt ggacttgaca
     2821 gaatccccaa ccagattacc tttggaacca atctggaaag ccagcggttg ggtagaaagg
     2881 ccatagtgaa aattgccgac aagtactttg aacgcgagga gattaaccga atagccattg
     2941 tggcgcccca tgccaagctc aacatcatca gggattatga ggtggttgag aaaatgctgg
     3001 ttgaggttcc cgaccatatt acgggcatag ttaggtgtgt gaaccctaaa tgcattacca
     3061 actttgagaa tgttgcccca aaatttgagg tggttgacaa gaaaaatgtt agcctgaaat
     3121 gccattactg cgaaaaaatc accgatcagg aacatatgga ataccgctag ggcgggaatt
     3181 atagatggaa tactttggaa ggaggtttcg ccgctaatcg aaggctgtag aaaattatgc
     3241 tgaacttctg gcccgagcag tagtggtaga aagcatcatg tagcgcgaaa gaatgtgcca
     3301 atgaattaaa aattaatttt taggcacata ctcccagaaa accttgatgt cgtcctgatt
     3361 caacctggca ttgagccagt tggaaatctg ctttttttcg gcaggggtta tcccggaggt
     3421 tataccgaaa aatactaccg tggttttatc gggttgagaa ctgtcggttt tatactggaa
     3481 ggtttcggca taggagcagc tggaaatctt agggaaaaaa gcttgaatct ccagcaggag
     3541 ggttttgcct agctgccttt tcctggagat actatcaatt tgggcatctt tatccctgat
     3601 aatcatattt aagcgggtta gctcagacct aagcttaata acctcactgt cggtttcatt
     3661 tgagctattc aaggctaaac cttgctcaat aagtattttg gcatcatcga gatcaaaatt
     3721 gctcgccttt tgggtaatcg cttctttttg cttttcagtt aagcttacac ctccataggt
     3781 tagagtgatg atttttttac gtacatcaat gttatttcta agtaggtagt tgccttcaaa
     3841 ggaacttaca ttcgaaatgt accggctagc attcaggtta aatttctcat tttgcaccag
     3901 cctgtagcca aaataaatgc tcggaagcag caccacgatt attaccgtag aaatccatct
     3961 gtttatgctt ttcttttgcc tgacatccat gtcagcaatt atcggaaatt taagaatttg
     4021 agaaatggct acagatgaaa tacctataaa aaccgtatta atggtgaaga ggtagaacgc
     4081 cccaaagaaa aagttgaact gccctgtggc caatccgtag cctgccgtgc aaagaggagg
     4141 cataagcgct gtagcaatag caacaccggg gataacattt cccttttgct tactgctaat
     4201 ggcaacgatt ccggccagcc cgccaaaaag agcaattatt acgtcataga ttgtgggagt
     4261 tgttcgggat agcagctctg aatgtgcagt tgatattgga cttattgaaa aatagagggt
     4321 tgaggcaaaa agcccggcaa gcacggcaaa accataattt tttaaagaaa ttttaaagag
     4381 cttcatgttg taggttgcta tgctgtagcc cataccattt ataggcccca ttagtggcga
     4441 tatcaacata gcgcctataa ttaccgctgt agagttcata ttcaggccaa ccgatgctat
     4501 aatgatagca aacattaaaa tccacaggtt ggttcccctg aacaccacgt cctttacgat
     4561 gctttcatga atgcgatcat agctgtcgag ctcggactcg aggttgaagt agtgaaaaaa
     4621 tttgctgatt acactttgtg gtttcatccg tacaaaattt ttaaatttct gattacaaat
     4681 accttgaccc atacctttaa ggcatcgggg ttgggcggat ggaacaccca tattttgcta
     4741 acgcgtgttt gagcaagcta cattataagt atgtttcata tcattgaaag cttactttta
     4801 cctgtatcgg tgattgccta acaagctccc cgaaattaat aaaaaaatcc taaaggttga
     4861 atgcctggca taaccctgtc gaagcgtaag gaattaaagg gaaaaggata aatgttagca
     4921 cgtgtgctga tgagcattag actcgatttc tcgatttctt cctgttatta gcaaggtaac
     4981 ttcttgattt ttattgttcc atgtaattga gaaccctgac catgtgaaag gttaattttt
     5041 ttatccctta tattttgatt ctctttgtta aattttgttg aaaaatgaat ttctgttttt
     5101 caaccttttt taacttttgc tgtcatacta ggcaaaggtg ttaactagta aacttaaaat
     5161 aaaatcagca tcaaatgaca gcattttcta aaaggcttat caggatatct actacggttg
     5221 ctattgctct acttttagtt gggattattc tgtatcccaa ggtaaaagat gcttcaaata
     5281 gtgattctaa agataaagta ccaggacgaa tggccatgcg gggtggggga ggccagccgc
     5341 tttttgccaa cggttttatt attgttccaa catccatgag cgagttgatc tctaccaccg
     5401 gcagcttact cccggatgag gaggttgacc tttcatttga aacatccggg aaggttgtag
     5461 ccatttactt tgaggaggga acaaaagtga aaaagggcga tttgttggcc aagataaacg
     5521 atgccccgct gcaggctcag cttcagaagc ttcaggctca acgcaagctg gccgaggaaa
     5581 agttgttccg ccagaagcag ctacttgagc gcgatgctat cagtcgggag agctacgacc
     5641 aggtatctac ggagctacag gccattgagg ctgatatcat gctcattaaa gcgcgtattg
     5701 ccgaaacgga gctacgggca ccatttgatg gaattgtcgg gttgcgcttg attagcgagg
     5761 gtgcctacac ttcaacccaa accccaatag cccatctggt taaggttagc ccccttaaaa
     5821 ttgaattttc cattcccgag cggtacgccg gggaggtaac cccgggctat gacattagct
     5881 ttgttattga cggaattgaa agtaagttta ccggaaaggt ttacgcggta gcacccaagg
     5941 ttgacatggc tacacgggcc atcgtggtta gggctatgta cccaaatccc aacgaagagc
     6001 tgaagcctgg tcgttatgcc agcatcaccc tgcagctttc aaaaattgaa aacgctgttt
     6061 caatccccac cgaggcgcta atccccgaaa tggagggtga aaaggtattc atctacagga
     6121 atggaaaagc cgaacaggtt agggttacca ccgggctacg caccgagtcg cgcattcaag
     6181 tgcgcacagg cctcaacttt ggcgacactc tgcttacctc cgccattctt cagctgcgac
     6241 agggcattcc tgttcagctt gatacaatta ttactaatgc aataaatctc cagtaaagca
     6301 catgagtctt tcatcattaa gtataaatcg cccggtactt tccaccgtat tttccctcat
     6361 catcctgcta cttggcgcaa taggtataac ctttctgggt gttagggaat ttcctagcgt
     6421 tgacccgcca atcatctcgg tgagcacatc ctaccccgga gccaactccg atgttataga
     6481 aactcagatc accgaacccc tggagcagtc aatcaacggc attcccggaa ttcgcaccct
     6541 taccagccgg agtagtcagg gaagcagcag gattaccgtg gagtttgaac tttcagttga
     6601 catggagact gctgcaaatg acgtgcgtga taaggtttcg caggcacagc gtcttttgcc
     6661 acgcgattgc gacccgccaa ctgtttcgaa ggccgatgct gacgcgcgtc ccataatgaa
     6721 tattgccatt aacagcaaga agcgctcgct gctcgagctt tccgaaattg ccgatcttac
     6781 tctgaaagag cagctgcaaa ccatttcggg cgttagctcg gttggcattt ggggcgagaa
     6841 aaggtacgcc atgcgtatat ggctcgaccc tgcgaagctg gccgggtacc aaatgactcc
     6901 gcttgacgtt agaaatgcgc tgaatcgtga aaatgtggag cttcctgcgg gcagtattga
     6961 aggggcaaac accgaactta ccattcgggt gctgggcctt atgtcaacac ccgaggaatt
     7021 taacaattta attcttaaac gttctggcga gcagctgatt agggttcgtg atatcggaag
     7081 ggctgagctg gcccccgaag acctacgaag cattatgaaa atgaacggtg tgcctatggt
     7141 ttcggtagtt gttattccac agcccggggc aaaccaaatt gaaattgtgg atgaggttta
     7201 caagcgtatc gattttatta aaaaagacct gcccgatgat gtaaatattg tggtgggttt
     7261 cgacaacacc cagtatataa ggtcgtcaat taaggaggtg caaaccacca tttacattgc
     7321 attttttctc gtggtggtta tcatttttgc cttccttcgc gattggcgaa ccacccttat
     7381 ccccatcctg gtaatccccg tttcgctggt tggcgtattc ttcataatgt accttgcagg
     7441 gttcagcatc aacattttaa ccctgctggc aatagtgctc tccattggtt tggtggttga
     7501 cgatgccatc atcatgatgg agaatatcta cgttaagatt gagcagggta tgaccccgtt
     7561 ggaggcgggt gttaagggat cgaacgaaat attttttgcc atcattgcca caaccattac
     7621 cttgatttcg gtattctttc ccattgtttt tcttgagggt atgacgggga ggctgtttcg
     7681 ggagtttagt attgtaattg caggagcagt tgccatttcg gcttttgttg ccctgacttt
     7741 cacccccatg ctttccacca agctccttaa aacccggcat accaggagca aggcgtatag
     7801 ctacaccgaa aagtttttta cctcgttgaa tagtgggtac aggaattcgc taagcagctt
     7861 tctgaagcat aggtacctgc cagttgttat tcttgtggta gctgctggcc tgatagttct
     7921 actttggaaa agtatccctg ccgaaatggc tcctatggag gatagatcgc aggtaagcct
     7981 tagcgttaca ttccccgagg ggacaaccta cgactatagc gcagcgtatg ttgaggatgt
     8041 ggctgctttg gttgataagc tggttcctga agctgagaag gccacttcgc gtgcatggag
     8101 aggaggtgct tccatggatg tagttctgat accgccatcg gagagagacc gctcgcagca
     8161 ggatattgcc aacatgctaa ccgctgaact cagaaaaaag accaaagcca gggtgaatgt
     8221 aattcaacag tcaacatttg gtggacggcg ggccagcttg ccaatccagt acgtgcttca
     8281 agcccaaaac attgacaagc tgcgtgaaat actccctgct ttcatggccg aggtgcagga
     8341 taatccaact tttaccatgg ccgatgtaaa cctgaggttt accaaacccg aacttcaggt
     8401 tgaaatcaac cgtgacaagg ccaacatgct tggggtttca acccaaaata tcggtcaaac
     8461 ccttcaactc gcccttagcg ggcagcgttt tgggtacttc atcatgaacg ggaagcaata
     8521 ccaaattctt ggcgagctgc cacgtgctac ccgcaatgaa cccctcgatt tgaaatcgct
     8581 ttacgttcgc agcgatgcag gacaaatggt acagctcgat aattttgttg aggtgaagga
     8641 gtcgaccgct ccccctcagc tttaccgcta caacaggttt gtttcggcaa ccatatcggc
     8701 cgggctggca gagggaaaga ccattagcca gggaattgag gagatggata aaattgccgc
     8761 caaaactctc gacgatactt ttagaacagc tctggctggt gactcgaagg acttcacaga
     8821 aagttcatca agccttatgt ttgcatttgc catggccatt gtgctcattt tccttgttct
     8881 agctgcccag ttcgagagct ttaaggaccc tgtaatcgta atgatgaccg ttccccttgc
     8941 cttggccgga gctttaatct tcatgtggta ctttaacatc accatgaaca tattcagcca
     9001 gattggaatc atcatgctta ttgggttggt ttcaaaaaac ggaattctta ttgttgagtt
     9061 ttcgaaccaa cgtaaaaatg ccggcatgag caaacttgat gctgtaaagt atgctgctgc
     9121 agcacgtttt cgtcctattc tgatgacgag cctatcgaca attttgggaa ttctacccct
     9181 ggcgttaggt ctgggcgagg gagcgcaaag tcgcgttgca atgggaactg ctgtggttgg
     9241 aggtcttacc gttgctacct tccttacact ttacgttata cccggtgttt acctgctgat
     9301 ttccagcgaa tctgctgcag gtaaagcggc aaactcaaaa gggcgggaag tcgaaggtat
     9361 tgagcagtag ccgctaattt ggttaatgtt ttgatggggt ttccacactt ggacgtagct
     9421 acttttatag atttgcattc tgaaaccaat tgatattcaa actgataaat attaaatact
     9481 gatgaatgga aagtttgatt taatgaacca aacaacaccc tcagcattgg cagctccaat
     9541 ggccaaaagg atttttacta ttacccttct gatatgcctg tgtagcggct tatccaaatc
     9601 acaggagcta ttcgatttga gctcatgcat taaggctggc ctcgagcaaa atttttctat
     9661 actgatagcc cgcaatcagg aaaaaattgc ctcaaataac tataccgtag ggaatgccgg
     9721 atacctgcct acgctcagcg gctcgggaag atacggagga actagcaaca ccactcacca
     9781 gtcgtttacc gatggaacgg agagcgaggc caatggtgt
//
LOCUS       DAOOQX010000002         9293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_9204_length_9293_cov_3.276142, whole genome shotgun sequence.
ACCESSION   DAOOQX010000002 DAOOQX010000000
VERSION     DAOOQX010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9204_length_9293_cov_3.276142"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /gene="nadE"
                     /locus_tag="PLX53_00045"
     CDS             <1..751
                     /gene="nadE"
                     /locus_tag="PLX53_00045"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOY71500.1"
                     /translation="SGGIDSAVVLALAHSALGSDAIRVLLLPSQYSSKHSVDDAVTLA
                     TRMGIRYDTISIQPIFDSFKSQLADLFQGLPEGVAEENIQARVRGTLLMAISNKFGHL
                     LLNTTNKSEAAVGYGTLYGDMNGAIAVLGDVYKTDVYNLASYINRNQEVIPPSIISKP
                     PSAELRPNQKDTDSLPPYDVLDRILFAYIEQNHSVKDIIGMGFDSALVHRVIRMVCTS
                     EYKRFQAPPILRVSSKAFGYGRRMPLVSSWT"
     gene            962..1672
                     /locus_tag="PLX53_00050"
     CDS             962..1672
                     /locus_tag="PLX53_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOY71501.1"
                     /translation="MSKKLQHQVDEDSVFEGVCGLFPNLKPEEREKMRNNFSCMIVPK
                     GKIIYREGDTPPGLVCLSKGIVKLYKEGVGGRDQIVRMAKPVGFMGYRALFADENYTA
                     TAQALEDSVVSIIERNALMEILRNNAELTLHILKSVASELGFSTNRTVTLTQKHVRGR
                     VAESLIFLRDTYGFEDDGVTLKAYLSREDMANLSNMTTSNAIRTLSSFAEEGIIELDG
                     RRIKILDQYKLERVSNLG"
     gene            complement(1681..>2075)
                     /locus_tag="PLX53_00055"
     CDS             complement(1681..>2075)
                     /locus_tag="PLX53_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HOY71502.1"
                     /translation="LMPVSTLKSKVIQVKHLQPGETVGYNRMGKINKPTTVAAIPIGY
                     ADGLNRKLGNGIGKMMINGVLVPTIGNISMDTCTLDATGVDVKVGDDVVIFGSNPTIM
                     EVSQALNTIPYEVLTSVSQRVKRIYFQE"
     assembly_gap    2076..2085
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2086..4163)
                     /locus_tag="PLX53_00060"
     CDS             complement(<2086..4163)
                     /locus_tag="PLX53_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOY71503.1"
                     /translation="MQPYTTGQIAQILNAKLEGDPNLEISTIATDSRTPSKSKGTLFV
                     AIVGKHHNGHKFISELYNFQHIRAFMVSDTLGLAEKFPQATFIVVKNTLHALQKLATY
                     HRAQFKYPVVGITGSNGKTIVKEWLNQLLSPDYSIVRSPKSYNSQIGVPLSVLQMDSN
                     FNLGIFEAGISLPGEMLRLQPIINPQVGIFTNIGLPHQENFEDLDEKITEKLKLFKSS
                     ELLIYCKDHELVDRIIYNDDTLSGVKLLSWGVHPQSTLHVESTEEHGNGRVIRVKYGE
                     ETFSFEIPFTDQAFYENAMHCLSLLFYLNIDRTTIADRMAKLSPVAMRLELKKGVNGC
                     TIINDSYNSDLGSLSIALDFLLKQKQHDNRVLILSDILQSGYAPELLYSMVAQLVEEK
                     EISHIIGIGKNISRYAHLFDISKEFYSNTLEFTQSITRRQFSNSAILIKGSRNFHFER
                     IVAILEEKVHRTVMEINLNSMVHNLNHYRNQLNPGVKLMVLVKAFSYGSGSYEIANLL
                     QFNRVDYLGVAFADEGVSLREAGIALPIIVLNPSYGAYELMIENRLEPEIYNFEGLAE
                     FANALDRMGVNEYNIHLKIDTGMHRLGFTGEQLPELTSTIQSLKSIRIASIFSHLAAS
                     EDSGQDEFTQSQIALFSTISDEIISAIGYKPLRHILNSAGIERFPHAQFDMVRLGIGL
                     HGISPTQQDKL"
     gene            complement(4258..5334)
                     /locus_tag="PLX53_00065"
     CDS             complement(4258..5334)
                     /locus_tag="PLX53_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOY71504.1"
                     /translation="MKILDWYIIRKFIGTYIYAIILIVGIAVIFDFSEKIDDFMEKGA
                     PFNEIIFRYYLNFIPYFANLFSSLFVFIAVIFFTSNMAYNTEIIAILSSGVSFLRMMV
                     PYFISATIIAIFSLVLTNFIIPPANAVRIDFENKYVKKAYVSSERNIHRQVRPGIFVY
                     MRSFNSYSNIAYNLSIEHFDNNRLKSKLIADYAQWDSTKQTWTIKNYYIRDYLERGEK
                     ITRGQSIDSTLYLTPADFKRRSNVIETMNLFQLNDFIDEQRLQGADNIEALLIEKHSR
                     FANPFSAFILTLIGVSLSSRKVRGGIGLHIGFGLALSFSYILFMRFSTIFAVSGHLSP
                     FIAVWIPNIIFAAIALGLYRLAPK"
     gene            complement(5497..6627)
                     /gene="tgt"
                     /locus_tag="PLX53_00070"
     CDS             complement(5497..6627)
                     /gene="tgt"
                     /locus_tag="PLX53_00070"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOY71505.1"
                     /translation="MDFRLIGTDSQTSARAGEIATDHGVIETPIFMPVGTVGSVKGVL
                     HRELENDIDARIILGNTYHLYLRPGLEVLANAGGLHRFSSWSRPILTDSGGYQVFSLA
                     SNRKLNAEGASFRSHIDGSSHFFTPERVMDIQRVIGADIVMAFDECPPGDAEYSYACK
                     SLDLTEQWLKRCVNQFATTEPHYGYSQSLFPIVQGCVYPDLRRRAAEYAVSFNCDGYA
                     IGGLSVGEPAEEMYRMIEVVNYILPKNSPRYLMGVGTPENILEAIALGVDMFDCVLPT
                     RNGRNGMLFTSQGIINLRNEKWKNDFSPIDPLGLTFVDSRYSKAYLRHLFVAKEMLGP
                     QIASIHNLGFYLWLVREARIHIVRGDFLEWKKSMVSRLSQRL"
     gene            7159..8307
                     /locus_tag="PLX53_00075"
     CDS             7159..8307
                     /locus_tag="PLX53_00075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY71506.1"
                     /translation="MKEILSSIETYVLVVGILFIAALVVQLYYYLVVYARVACSKSYK
                     RLLSPEAPKPPVSVVICARNEEENLRHFLPRILEQDYPEFEVVVVNDCSCDESEVLLD
                     RLSKEYPRLQVRTIKEDEKFSHSKKLALTVGIKAARYDWLLLTDADCIPQSDKWIATM
                     ASNFDANAGVVLGYGGYLHQKGWLNKFIRFDTLYIAMNYLGFALMGKPYMGVGRNLAY
                     RKDLFFNNKGFASFVQLDSGDDDLFVSQVATRQNTRVDFRKDAHTRSVPRSSHHLWLQ
                     QKIRHTSTSRYYKPGVKIRLTLEPLSRFLLLASAICLVAFRFFPYWIVALVLFKLSVQ
                     LIIIKSVMVRLGERKILLVSLLWDIYSLYFYPKLLLINRFTRRKTTWR"
     gene            8298..8912
                     /locus_tag="PLX53_00080"
     CDS             8298..8912
                     /locus_tag="PLX53_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOY71507.1"
                     /translation="MEIAPENLSEKARYDYELVNRALEGDQKSYAELMERYRDAIYFM
                     LLKMVNNKSDAEDLTLEAFGKAFKKIHQYTPNYAFSTWLFKIATNNCIDFIRKRKANI
                     VSIDQNIDDQEGQYISPTSMLSSGNPDPEEKLIKEQNIQLIQDIVSKLKPRYRKLVEL
                     RYFKEYSYEEIADELNLPLGTVKAQLFRARELLSNILNNLPPHH"
     gene            9064..>9293
                     /locus_tag="PLX53_00085"
     CDS             9064..>9293
                     /locus_tag="PLX53_00085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY71508.1"
                     /translation="MVNSSIVTQYFSGLNKDQLEKFERLDDLYGYWNAQINVISRKDI
                     DNLMVHHVLHSLSIARVINFARGTKIMDVGTGG"
BASE COUNT         2439 a         2074 c         2211 g         2559 t
ORIGIN      
        1 ttccggcgga attgattcag ctgttgtcct ggccttagcg catagcgcct tgggttccga
       61 tgccatcagg gtgctgcttt taccctcgca gtactcatcg aaacactcgg tggatgacgc
      121 cgtaaccctt gccaccagaa tgggaatacg gtacgacacc atcagtattc agcccatttt
      181 cgactcgttc aaaagccagc ttgccgacct gtttcaggga ttgcctgagg gtgtagccga
      241 ggaaaacatt caggcaaggg ttaggggaac gctgctcatg gcaatttcga ataagtttgg
      301 ccatttactt ttaaatacta ccaataaaag tgaagctgct gttggttatg gaacgcttta
      361 cggcgatatg aacggagcca ttgccgtgct gggtgatgtt tacaaaaccg atgtgtacaa
      421 cctggcaagc tatattaaca gaaatcagga ggtaatccct cctagcatta tctctaaacc
      481 gccatctgcc gagctacgcc ccaaccagaa ggataccgat tcacttcccc catacgatgt
      541 gctcgatcgg attctttttg cctacattga gcagaaccat agcgtgaagg acattatagg
      601 catgggattc gacagcgctc tggtgcaccg tgttattcgg atggtttgca caagcgagta
      661 caagcgattt caggcgcctc caattttaag ggtttcgtcg aaggcgtttg gctacgggcg
      721 ccgcatgcca ttagtttcat cgtggacgta attaccggtt gaaagaagcg gccattaatc
      781 ctacacgcta aacactatta ttgtttttgt ttttcaactt tttattgaat gcttgatatt
      841 ccatggccaa aaactttttt actttaaaag gatattatac agcatcgata gcgctgccta
      901 ttaaactttg ttaaaaacat gttttttaac tataaaaagc cctatttttg aaaaaaaggt
      961 tatgtctaaa aaattgcagc atcaagtcga tgaagattcg gtttttgagg gtgtttgcgg
     1021 tttgttccca aatctgaaac ccgaagagcg cgagaaaatg cggaacaact tttcctgcat
     1081 gattgtgcct aaaggtaaga ttatctacag ggaaggcgat acaccgcccg gcttggtttg
     1141 cctttcaaaa ggtattgtga agctttataa ggaaggggta gggggacgcg atcaaattgt
     1201 gcgcatggcc aaaccggtag gctttatggg ctacagggct ttatttgccg atgaaaacta
     1261 caccgccact gcacaggctc tcgaagattc tgtggttagc attatagagc gaaacgcgct
     1321 gatggaaatt ctcagaaaca acgctgagct taccctgcac attcttaaat cggttgcttc
     1381 cgagctcgga ttttccacta accgaacggt aacccttacg cagaagcatg ttcggggacg
     1441 cgtggccgag tcgctcatct tccttcgcga tacatacggc ttcgaggatg atggggtaac
     1501 gctaaaggca tacctatcgc gcgaggatat ggccaacctg tcgaacatga ccacctctaa
     1561 cgccattaga accctttcga gttttgccga ggagggaata attgagctgg atggccgaag
     1621 aattaaaatt ctcgatcagt acaagctcga gagagtgagc aatttaggtt agctagccgg
     1681 ttactcctga aagtaaatgc gtttaaccct ttgcgaaacg gaggttagca cctcgtaggg
     1741 gatagtgttt agcgcttggg agacttccat gatagtcgga ttgctaccaa aaataactac
     1801 gtcgtctcca accttcacat caaccccggt ggcgtcgagt gtgcaggtgt ccatgctgat
     1861 atttccaatg gttggcacca gcactccgtt aatcatcatc tttcctattc cgttgcccag
     1921 cttacggttt aacccatcgg cgtagccaat aggaatggca gcaaccgtag taggtttgtt
     1981 tatcttgccc atacggttat acccaactgt ttcgcccggt tgcagatgct ttacctgtat
     2041 caccttgctc tttaacgtgc tgacgggcat tagctnnnnn nnnnnagctt gtcctgctgg
     2101 gtagggctaa taccgtgcaa cccaattccc agcctaacca tgtcgaactg ggcatgggga
     2161 aaacgctcga taccagcact attcaggatg tggcgaagtg gcttatagcc tattgcactg
     2221 atgatttcat ccgatatggt gctgaaaagc gctatttggc tttgggtgaa ctcatcctgt
     2281 ccggaatcct cgcttgcagc caggtgcgag aagatgcttg caatccttat actttttaac
     2341 gattgaatgg tggatgtcag ctccggaagc tgttcgccgg taaaccccag gcgatgcata
     2401 ccggtatcaa tcttcagatg aatgttgtat tcattcaccc ccatcctgtc cagggcattg
     2461 gcaaattcgg caagtccttc aaagttgtat atttctggtt caagccggtt ttcaatcatc
     2521 agctcgtagg cgccgtagga tggattgaga acaatgatag gcagagcaat accggcctca
     2581 cgaagcgata caccctcatc ggcaaatgcc actcccaggt agtcaaccct gttgaattgc
     2641 aggaggttgg caatttcgta tgaaccgctg ccataagaga aagcttttac cagcaccatc
     2701 agctttactc ccgggttgag ctggttgcgg tagtggttca ggttatgcac catcgagttc
     2761 aggtttatct ccatcaccgt tcgatgaact ttttcctcta aaatggctac tatccgttca
     2821 aagtgaaagt tccggcttcc cttgatgagt attgcggagt tgctgaactg ccttcgggtt
     2881 atgctttgtg tgaactcaag cgtattgctg taaaactcct tgcttatatc gaagaggtga
     2941 gcatagcgtg aaatattttt accaatccca atgatatggg atatttcctt ttcttccacc
     3001 agctgggcaa ccatggagta cagcagctcg ggggcatagc cgctttgcag gatatccgaa
     3061 aggataagta cgcggttgtc atgttgtttt tgcttgagta aaaaatcgag ggctatggaa
     3121 agcgagccca ggtcggagtt atagctatcg ttgataatgg tgcatccgtt tacccctttt
     3181 ttcaactcca gcctcatggc aacggggctt agcttggcca tacggtcagc gatggtggtt
     3241 ctatcaatat taaggtagaa caacaagctt aggcagtgca tggcattctc gtagaaggcc
     3301 tggtcggtaa aaggaatctc aaagctgaac gtttcttccc catactttac ccgaataact
     3361 ctaccgttgc catgctcctc cgtactctct acatgcaggg ttgactgggg atgaacaccc
     3421 catgaaagca gcttaacccc actaagcgtg tcatcgttat agatgatacg atcaaccagc
     3481 tcgtgatcct tacagtaaat aagcagctcg gatgatttga aaagtttaag cttctcggta
     3541 attttttcgt ccaaatcctc gaaattttcc tgatgtggaa ggccaatatt ggtgaaaatt
     3601 ccaacttgag gattaatgat aggttgaagt cgcagcatct cgccaggaag ggaaatgccc
     3661 gcttcaaata tcccgaggtt gaagttgctg tccatttgca acaccgaaag ggggactcca
     3721 atttgggagt tgtagctttt ggggctgcgt acgatgctgt aatcgggcga aaggagctgg
     3781 tttagccact ccttaacaat ggttttgccg ttacttccag tgattccgac aactgggtac
     3841 ttaaactgtg cccgatggta ggtggctagt ttttgaagtg catgcagggt attcttaacg
     3901 acgataaagg tagcctgggg aaatttttcg gccagcccga gggtgtcgct aaccatgaat
     3961 gcccttatgt gctgaaagtt gtaaagctcg ctgatgaatt tatgaccgtt gtggtgtttc
     4021 ccaacgatgg caacaaaaag cgttcccttg cttttggatg gggttcggct gtcggtggca
     4081 attgtgctga tctccaggtt aggatcacct tcgagtttgg cgttaagaat ttgtgctatt
     4141 tgccctgttg tgtaaggttg catagtattt ataggtggat actccgtaca aatttacacg
     4201 aaatttccaa tgttgattct gctcagcatg taatacagga tgattaagct acagaattta
     4261 ctttggcgct aaacggtaga gccctaaggc gattgcagca aagataatat ttggtatcca
     4321 caccgcaata aacgggctga ggtgcccgct caccgcaaaa atggtggaga agcgcatgaa
     4381 taggatgtag gagaagctaa gggctaggcc gaaacctata tgcagcccta tgccgccgcg
     4441 aactttccgt gacgaaagcg aaaccccaat aagggtgaga atgaaagccg aaaagggatt
     4501 ggcaaagcgg ctatgcttct ctattaaaag tgcctcaatg ttatcggcac cctgcagccg
     4561 ctgctcgtca atgaagtcgt ttagctgaaa aaggttcatt gtttcaatca cgttcgaacg
     4621 tctcttaaaa tcagcgggag tcaggtataa agtgctatcg atggattggc cccgagtaat
     4681 cttttcgccc ctttcaaggt aatcgcgtat gtagtagttt ttaattgtcc aggtttgctt
     4741 ggttgagtcc cactgggcat aatcggcaat cagcttcgat tttagccggt tgttatcaaa
     4801 atgctcaatg gaaaggttgt aggcaatgtt gctgtagctg ttaaatgaac gcatgtaaac
     4861 aaagattcca ggtcgaacct gcctatggat attgcgctcg gagctcacgt aggctttttt
     4921 tacatactta ttctcaaagt caattcttac agcgttggct ggaggaatga tgaagttggt
     4981 gagcaccagg gagaaaattg caatgatggt ggcggaaata aagtatggca ccatcatccg
     5041 taaaaagcta accccgctgc tcaaaatggc tattatttcg gtgttgtagg ccatgttgga
     5101 ggtaaagaat atcaccgcta taaaaacgaa tagcgagctg aacaggttgg caaagtaggg
     5161 gataaagttt aggtagtacc ggaatatgat ttcgttgaag ggtgcaccct tttccatgaa
     5221 gtcgtcaatt ttttccgaaa agtcgaatat aacggctatg cctacaatca ggataatggc
     5281 atatatgtat gttccaataa acttgcggat tatgtaccag tcgagtatct tcacggcatt
     5341 aagttttgag ggcgaaaatt agtaaatatt gttacttgat tgtatgccag ctaatactta
     5401 gcggctcttt ggctaaagaa tacttcattt ttttacattt tgagagcagc ttcaccttcc
     5461 tgaagattga gttttgtagc tttcgaatgg cacaggctac aatctttgtg aaagtctgct
     5521 aaccatactt tttttccact ccaaaaaatc tccccgaaca atatgtattc tggcctccct
     5581 aaccagccag aggtagaacc ccaggttatg gatgctggca atttgtgggc ccagcatttc
     5641 cttggccaca aacaggtgcc gcaggtaagc tttggagtag cgtgaatcaa caaaagttag
     5701 gccaagaggg tcaatgggtg aaaaatcgtt tttccatttt tcgtttcgca ggtttattat
     5761 gccctgtgag gtgaaaagca ttccattacg gccattccgg gtgggaagaa cgcagtcgaa
     5821 catgtcaact cccagggcta tagcctccag aatattctcg ggtgttccta cgcccatgag
     5881 gtagcgaggg gagttctttg ggagtatgta attcaccacc tcaatcatcc ggtacatctc
     5941 ctcggcaggt tcgcccaccg aaaggccgcc aattgcatac ccatcgcagt tgaacgaaac
     6001 ggcatactct gcggctcgcc ttcgcaaatc ggggtacacg caaccctgta ctatcgggaa
     6061 aagggattgg ctatacccgt aatgcggttc ggtggttgca aactggttta cacatctttt
     6121 aagccattgt tcggttaaat cgagcgactt gcaggcatag ctgtactcgg catccccggg
     6181 ggggcactcg tcaaatgcca tcacgatatc ggcgccaatt acccgctgaa tgtccattac
     6241 cctttcgggg gtgaaaaaat gagatgaccc gtcaatgtgc gagcggaacg aagcgccttc
     6301 cgcatttagc ttccggtttg aggccagcga aaaaacctgg tagcccccgc tgtcggtaag
     6361 tatggggcgg ctccatgagc taaaccggtg aagcccaccg gcattggcca aaacctccag
     6421 cccgggacga aggtagagat gataggtgtt gcccagaatt atccgggcgt caatatcgtt
     6481 ctccagctcg cggtggagca cccccttaac actcccaacg gttccaacag gcatgaaaat
     6541 cggagtctca atcactccat gatcggttgc tatttcacct gcccgtgcag aggtctggct
     6601 gtcggtgcca attagtcgaa agtccatcct gtatattttc gccgcaaaaa tagcaaaatt
     6661 agctatgtgg ggcggtatgg ttttgcccta cgaacaataa tttgtatata gaaatgggta
     6721 gcttgttttg ttaccggtag ttttacaggc tgtagcgttt gttaaaaatg cccaaaaatt
     6781 ttacacgtca aattgagtgt ccatgtgaga ctgcttgcaa ctttcaggta agcataaata
     6841 gtagagtttc ttttattttt acagcatgac aaggcacaag caggtgtctt gtcatgctaa
     6901 tcatccgaac tcgccgatat gctattaacg aggcttttga gttgtttggc ctggttcggg
     6961 ctatagattt cgcccaaaac cctagctact tttgctaccg cctcaacaac ctgccggcgc
     7021 tatttcacct gttacctcat gtttgtaatc gcctaaatgt ctggttcaaa caggtagttt
     7081 agcaaattac tcctttattg ttgggtaaac ccaaaaatgt ttgatattat tgctggattt
     7141 tttaaacaat gaatagcggt gaaagaaatt ttaagcagca tagagaccta tgtgctggtg
     7201 gtgggcattt tgtttattgc cgccctggtt gttcagctct actactacct ggtagtttac
     7261 gcccgagttg cctgtagtaa aagctacaag agattgttga gccccgaagc tcccaaaccg
     7321 cctgtttcgg ttgtgatttg tgcccgaaat gaggaggaaa atttacgcca ttttcttccg
     7381 cgtattctcg aacaggatta ccctgaattt gaggtggttg tggttaacga ctgctcttgc
     7441 gacgaatctg aagtgctgct tgataggctc agcaaggaat atccccgttt gcaggtgcga
     7501 acaatcaagg aggatgaaaa gttcagccat tctaaaaaac ttgcgttaac agttggaatt
     7561 aaggcagccc ggtacgattg gctgctgctt accgatgccg attgcatccc gcaaagcgat
     7621 aagtggattg caaccatggc ttcaaatttt gacgcaaacg caggtgtggt gctgggctat
     7681 ggtggctatc ttcatcaaaa aggctggctt aacaagttta ttcgcttcga cacactctac
     7741 atagccatga actacctggg ctttgcccta atgggtaaac cctacatggg tgtaggacgc
     7801 aaccttgcct accgaaaaga tttgtttttt aataataagg gatttgcctc atttgttcag
     7861 ctcgattccg gcgatgatga tcttttcgtt agccaggtgg caacccgtca gaacacgcgt
     7921 gtggatttcc gcaaagacgc ccacacgcgt tcggttccac gcagctcaca ccacctttgg
     7981 ttgcagcaaa agattaggca tacctcaacc tcgcgctact acaaaccagg agttaaaatc
     8041 aggttaacac ttgaaccctt gtcccgtttc ctgctgctgg catcagcaat ttgcctggtt
     8101 gcctttcgtt ttttccccta ctggattgtt gccctagtcc tttttaaact atctgttcaa
     8161 ctaattatca taaaatctgt tatggtaaga ttgggtgaac gaaaaatttt gctggtctcg
     8221 cttttgtggg atatatattc actgtatttt tacccaaaac ttctacttat taatcgtttt
     8281 accagacgga aaacaacatg gagatagctc ccgagaatct atcggaaaaa gcgcgctacg
     8341 actatgagct ggttaacagg gcactcgagg gagatcagaa gtcctatgcc gagcttatgg
     8401 agcgttatcg cgatgccatt tactttatgc tgctgaaaat ggtgaacaac aagagcgatg
     8461 cggaggacct gacccttgaa gctttcggta aggcatttaa gaaaattcac cagtacaccc
     8521 caaactacgc cttcagcacc tggcttttca aaattgctac caataactgc atcgatttca
     8581 ttcgtaaacg taaagccaac atagtttcca ttgaccagaa tatagatgac caggaaggcc
     8641 agtacatttc acctacctca atgctttctt ccggaaaccc cgaccccgag gaaaagttga
     8701 taaaggaaca gaacatccag cttattcagg acattgtttc caagctaaag ccccgctaca
     8761 gaaaacttgt ggagttgagg tacttcaagg agtactcgta cgaggagatt gccgacgagc
     8821 ttaaccttcc attgggaacg gttaaggctc aacttttcag ggctcgagag ctgctttcca
     8881 acatcctgaa taatttacca ccccaccatt aaaccaaaaa tggctggcag tgcgtggtag
     8941 aaagggttgg taaccgcatg ttatctagta gcggccaaac attatggcaa taggtaaact
     9001 cctaaatttt tgcttggtaa ttattttaca ttattgataa ctattaagtt ataagttgga
     9061 gaaatggtga acagcagtat agtaacccag tatttttcag gtctgaataa ggaccagctg
     9121 gagaaatttg aaaggctcga tgacctctat ggatactgga atgcacaaat caacgtaatt
     9181 tcacgtaagg atattgacaa cctgatggtt caccacgttc tgcattccct gtcaattgcc
     9241 agggttataa actttgcaag gggaacaaaa ataatggatg tgggaacagg ggg
//
LOCUS       DAOOQX010000003         8593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_10168_length_8593_cov_4.025182, whole genome shotgun sequence.
ACCESSION   DAOOQX010000003 DAOOQX010000000
VERSION     DAOOQX010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10168_length_8593_cov_4.025182"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PLX53_00090"
     CDS             complement(<1..945)
                     /locus_tag="PLX53_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOY71509.1"
                     /translation="MMRRSDIEVMAPAGNFESLMAAIQGGADAVYFGVGKLNMRAKSS
                     VNFLEDDLYEIVRICSASNVKTYLTVNTVLYDEDIDDMHRLIDTAKKARVSAVIVSDQ
                     AAMEYARQVGVEVHLSTQLNISNTHSLRFYSQWADVVVLARELNLQQVKLISEGIHKH
                     NINGPSGNKVRLELFVHGALCMAISGKCYLSLHQKNYSANRGECLQICRRSYTVTDNE
                     TGSQLEIDNEYIMSPKDLCTISFLDQILDAGIRVLKIEGRARGPEYVKTVTSCYAEAV
                     EAIVSGQYTPDKVEGWTKRLTSVFNRGFWDGYYLGRRLG"
     gene            1199..2101
                     /locus_tag="PLX53_00095"
     CDS             1199..2101
                     /locus_tag="PLX53_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOY71510.1"
                     /translation="MKPTLIILAAGMGSRYGGLKQVDGLGPYGETIIDYSVYDAIRAG
                     FGKVIFVIRRSIEEDFMKIFGNRFSRDVNFDIVFQELDMLPDNITCPPGREKPWGTAH
                     AVWVARKMVNEPFAVINADDFYGYDTFKTLANELSRSNLKNGQYFMVGFRLNNTLSEQ
                     GSVSRGACEVDNASFLKSIVEFTRIQRVDGKIFNIDDSGNRIELDGSMLVSMNFWGFT
                     QDFFTLIETLLKEFLNGIPDHKKSEIYIPTVVNRAIQDGLATCKVLSTTSEWFGVTYP
                     GDKIHVVDQLRKLTLNGVYPSPLW"
     gene            complement(2204..3079)
                     /gene="deoC"
                     /locus_tag="PLX53_00100"
     CDS             complement(2204..3079)
                     /gene="deoC"
                     /locus_tag="PLX53_00100"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOY71511.1"
                     /translation="MDFFVEDKFNLEVNGEEVGRHLARIKDLALSRLGDTDKLKACFG
                     SIDLTTLNTTDTAERGKQFAEKVNGFTVDFPEMPCVAAICVYPTLVSSVKENLKAKGV
                     KIASVAAGFPSSQTFLEIKLSECRMAIGQGADEIDVVIPVGSFLQGDLYSVFSEVKQI
                     KETAGDIHVKVILETGALPSLKEIKLASFLAMEAGADFIKTSTGKLEPAATPEAVFVM
                     CTAIKEYFEKTNRKVGIKPAGGVVTSSEALVYYTIVEQVLGSSWLSPDLFRLGASRLA
                     NNLLSDIYGRAVSYF"
     gene            complement(3118..3444)
                     /locus_tag="PLX53_00105"
     CDS             complement(3118..3444)
                     /locus_tag="PLX53_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOY71512.1"
                     /translation="MTFEEAQKEVDSWIRDHGVRYFSELTNTVLLMEEVGELARIIAR
                     KFGEQSFKDNESADGLSDEMADVLFVLICLANQTGVSLSEAFEKNIEKKTQRDSQRHK
                     NNPKLF"
     gene            complement(3447..3902)
                     /gene="dtd"
                     /locus_tag="PLX53_00110"
     CDS             complement(3447..3902)
                     /gene="dtd"
                     /locus_tag="PLX53_00110"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOY71513.1"
                     /translation="MRVVIQRVKSAKVEVNNHEVGCIHKGLLVLCGFEVEESEEDLQW
                     MASKICKLRIFDDETETMNLSIKDISGEILVVSQFTLHASTKKGCRPSYVRAAPSSLA
                     ILFYNRFLNLLEIELGSKIAAGVFGKMMEVSLVNSGPVTIVIDSKNKEF"
     gene            complement(4037..4987)
                     /locus_tag="PLX53_00115"
     CDS             complement(4037..4987)
                     /locus_tag="PLX53_00115"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOY71514.1"
                     /translation="MESNIIINQLLAFIVLAVIGAIASWRKIINDEGKDFLSKLIIDV
                     TLPLLIVSTFLKLESNPRLLVNGLWLFVLTFVNIGVLYMAGTLSARAQKLSPSDSTVH
                     VLHTMFGNIVFLGFPLFDALFPNGVGVFYASVYQLASNFITYTYGTYKLSAGVQKSKL
                     GNFFNINTIALLLGILLMVLKVKLPAFILEPISSLGKCTSPLSMVYIGALLAGMNLKS
                     TFKTTSIYVLTINKLIIVPLLLGFIYSFLRRSFNLNLSIEAFVVLVMQAAMPCQTIVV
                     VMSRRYGGSHVLATGNLFISTIFSIISLPLVYHFLVSIYS"
     gene            complement(5279..5968)
                     /locus_tag="PLX53_00120"
     CDS             complement(5279..5968)
                     /locus_tag="PLX53_00120"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOY71515.1"
                     /translation="MKIPDTTSLQLKISNTHTINMNENRSIERKRFCISLFVSLIFIV
                     ALWCIKIFEIVEGISLYRLGVFPRQISGLPGIIFSPLIHSDFNHLISNSLSLLFLLIG
                     LLYFYRDLSYKVILFIWITSGLMVWVGARDSYHIGASGLIYGLASFLFFSGVVRHDQR
                     LMAISLIVVFFYGGLIWGILPVYPAISWEYHLFGGISGLIAAILFRNQGPPRKVWSWE
                     QEEDENTPENL"
     gene            complement(6003..8210)
                     /locus_tag="PLX53_00125"
     CDS             complement(6003..8210)
                     /locus_tag="PLX53_00125"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY71516.1"
                     /translation="MKFRIVSLCLLFAFRVGISQNIDSLLLKLYQESNDRSRVEILLN
                     ISSFYDQHDRNFIQAENYLYEALQIAQTAGDNTLLANTYNELGKFYRNQSKYPEALKY
                     HHLADSLAVKMGDCILQATTLNNIGVVYRRLDNHAQASEYHLRALNIAEGCKDTFNIS
                     VACNSLGNIFSLNGRYQQALSYFNRALALSISQNNLLGQAMNFNNIGEVYEFMGDYSN
                     AKYYYTKSLDINFKLKNLKGISINYNALGKIEMFNGNPENAYRLFQNALEIDRKLGDK
                     KFLADSYVNLAKACLALKKIDKAKIYLDSTITIAQQIGSPWHLQSAYEAYSDYFVQKS
                     DYLSALLSYKNASRFKDSLLNEKNARHLSTIQTIYETEKKEKEISYLLQEQALKQKEL
                     EKNNAQKNALIIGLVLSSLVVIVSLFAWRTKVKSNIVLSDQIKEIEDKNRLLEIQKEE
                     IEQQKIKIESNQAFIEQKNKNLEEAHKIIESYIENITDSIRYAEKIHESIQPSLSDML
                     SIFLDAFIYSKPKDIVSGDFCWLTRRDNLVFLALADCTGHGVPGAFMSTIGINILNQA
                     INEQHLYSPDKILTFINRTLINRLNKSTGEQVLKNSMDIAVCTFDTESLLLSYSGALI
                     PIFIQKGNSVEEVKPDSITLGTNFSHSASGFSLQNIQLNHGDWIYLSSDGFFDQLGGE
                     SRKKFTRKQFMQIVADSPNMKGNVKVDIIEKKFLQWMNRTPQLDDVLVWGFQV"
BASE COUNT         2573 a         1721 c         1654 g         2645 t
ORIGIN      
        1 ccccaacctg cgcccaaggt agtaaccatc ccaaaagccc ctattgaata ccgatgtaag
       61 ccgtttagtc caaccttcaa ccttgtcggg tgtatattgg cctgaaacaa ttgcttcaac
      121 tgcctcagcg tagcaggatg ttacggtttt tacatactcg ggacccctag cccgtccttc
      181 aattttaagc accctaatgc ccgcatcaag aatttgatcg agaaaggaaa tagtgcagag
      241 gtctttaggt gacatgatgt attcgttgtc tatttcgagc tgcgagccgg tttcgttatc
      301 ggttaccgta tagcttctcc tgcaaatttg caggcattcg cctcgattgg cagaataatt
      361 cttttggtgg aggctaaggt agcacttccc ggagatggcc atacaaagtg ctccatgaac
      421 aaaaagttca agcctaacct tgttccccga gggaccatta atgttatgct tatgaattcc
      481 ttccgaaatt aatttgacct gctgaaggtt gagctccctg gcaagcacaa ccacatcggc
      541 ccactgcgag taaaacctta acgagtgggt attgcttata tttagctgcg ttgaaagatg
      601 aacttcgact cctacctgac gggcatactc cattgcggcc tggtctgaaa caattaccgc
      661 ggaaaccctg gcctttttgg cagtgtcaat taacctatgc atgtcgtcta tgtcctcatc
      721 ataaagcacg gtatttacgg tcaggtaggt tttaacattg cttgctgagc aaatgcgaac
      781 aatttcgtaa aggtcgtctt caagaaagtt gacgctggat ttagcccgca tatttaattt
      841 accgactccg aagtaaactg catcggcacc cccttgaatt gcagccatca gcgattcaaa
      901 gtttcccgca ggagccataa cttctatatc cgaacgtctc atcattaagc ccttgtttct
      961 gcaaaagtag gtattttaac tgttaatacc tttaagctat ttgtatagaa agaaattcaa
     1021 tgtttaaact tttagtttaa ttgctgattc tatgtggcta tacatatgga ctatatcgtt
     1081 attattatca aattatattg caaaaaatat caaaattcga atggttggtt taccatagct
     1141 gtgccataaa tgctactttt gtcaacccta aaaatttaag tttgtaaagc ataccatgat
     1201 gaagccaacg ctaattatcc tggctgccgg aatgggcagc cgatatggtg gtttaaagca
     1261 ggttgatggt ctggggccat acggcgaaac cataattgat tactcggttt acgatgccat
     1321 tcgggctggt tttggaaaag ttatttttgt tatccgccgc agtatcgagg aagattttat
     1381 gaaaattttt ggaaacaggt ttagccggga tgttaatttt gatatagtct ttcaggagct
     1441 tgacatgctt cccgataaca ttacctgccc tccgggaagg gaaaaaccct ggggtacagc
     1501 tcatgctgtt tgggtagccc gtaaaatggt taacgagcct tttgccgtta taaatgctga
     1561 tgatttttat ggttacgata catttaaaac tctggcaaac gagctctcaa gaagcaatct
     1621 taaaaacggc caatacttta tggtgggatt caggctcaac aatacactct ctgaacaagg
     1681 gtctgtgtct aggggcgctt gtgaagttga caacgcatca tttcttaaga gtattgttga
     1741 gtttaccagg attcaaaggg ttgatgggaa aatatttaat atagacgact cgggtaaccg
     1801 catcgagcta gacggcagca tgctggtatc gatgaatttc tgggggttta ctcaggactt
     1861 ttttaccctt atagaaacat tgcttaaaga gtttttaaac gggatccccg atcacaaaaa
     1921 atcggaaata tatataccaa cagttgtcaa ccgtgccatt caggatggat tggcaacatg
     1981 taaggttctt tctactactt ccgaatggtt tggggttacc tatcctggcg ataaaattca
     2041 tgtggttgat cagctcagga agctaacgct taatggcgta tatccgtcgc ccttatggta
     2101 aaaagaggtt ttcagtagcc tagcccaggg ataaagcaac ctttggctaa ataagcgctt
     2161 tattcaatta tataaaaaaa acttgttctg tatgttctga gacttaaaag tagcttacag
     2221 cacgaccata aatatctgaa agaaggttgt ttgccagtct gcttgctccc agcctgaaca
     2281 ggtcggggga tagccaggac gaacccaaaa cctgctcaac gatggtatag taaaccaggg
     2341 cttcggaaga agttacaacg ccgcctgcag gcttaatgcc aactttacgg tttgtttttt
     2401 caaagtactc tttaattgcg gtacacataa caaaaacggc ctcgggtgta gctgcgggtt
     2461 caagctttcc tgtagatgtt tttatgaaat cggctccagc ctccatagct aaaaaagaag
     2521 ccagctttat ttctttcagc gaaggaaggg ctcccgtttc cagtattacc ttaacatgaa
     2581 tatcgccagc agtttccttt atttgtttta cctcgctgaa aacgctatac agatctccct
     2641 gtagaaaaga tcccaccgga ataaccacat caatctcgtc agccccctgg ccaattgcca
     2701 tcctacattc agaaagttta atctccaaaa aggtttgcga cgaaggaaat cctgcggcaa
     2761 cggaggcaat cttaacccct ttagctttta ggttctcctt tacggatgat actagtgttg
     2821 gatatacgca aattgcagcc acgcatggca tttcaggaaa atcaacagtg aagccgttaa
     2881 ctttttcagc aaattgtttc cctctttcag cagtgtctgt tgtgtttaag gttgttaggt
     2941 caatcgagcc aaaacaagcc ttcaacttat cggtatcacc aagccgggaa agtgcgagat
     3001 ccttaattcg tgccaaatgg cgcccgactt cttccccatt aacctcaagg ttaaatttat
     3061 cttcaacaaa aaaatccata ctgtataaaa tattaaaagt tcggttatgc tacaaagtta
     3121 aaataacttg gggttatttt tgtgcctctg ggagtctctt tgtgtttttt tttcaatatt
     3181 tttctcaaat gcttcagaaa gggatacccc tgtttgattg gccaggcaaa ttaaaacaaa
     3241 aagaacatcc gccatttcgt cggataagcc atcggcgctt tcgttatctt taaaagactg
     3301 ctcgccaaat ttgcgggcaa taatccttgc cagctcgccg acttcttcca tcagaagcac
     3361 ggtgtttgtc agctcgctaa agtacctcac gccgtgatcc ctaatccagg agtcaacttc
     3421 cttctgtgcc tcttcgaatg tcattttcaa aattctttat ttttagaatc tatcactata
     3481 gttacagggc cactgtttac taatgaaacc tccatcattt ttccaaaaac cccagcagcg
     3541 attttagagc ccagctcgat ctccaataaa tttaagaatc tgttgtaaaa caatatggcc
     3601 aacgaagatg gagcggcgcg cacataagat ggtcggcaac cctttttagt gctggcgtga
     3661 agggtaaact gactcaccac tagtatttct ccggatatat ccttaatgct aaggttcatg
     3721 gtttcagttt catcatcgaa tattcgcagc ttgcaaattt ttgaggccat ccattgtaaa
     3781 tcctcttccg attcctccac ttcaaaacca caaaggacca ataagccctt gtgtatgcag
     3841 ccaacttcat gattgtttac ttccacctta gcacttttaa cacgctgaat aactactctc
     3901 atggtaataa tagttaaaaa ttattccatt tgaaaatttg tgaatctacg cacaaaaact
     3961 atgtatatcg ttttgatttt ttatatttga ttcattatat gcaccttatc gttttataat
     4021 ttaataatat atttaactaa gaataaatac ttaccaaaaa atggtaaacc aaaggtagtg
     4081 agatgatgct aaagatggta gagataaata agttccctgt tgctagtacg tggcttccgc
     4141 cgtaacgtct agacattact acaacaatcg tctggcaagg catagctgcc tgcataacca
     4201 aaacgacaaa tgcctctatt gataggttta aattaaacga ccttctgaga aaagagtata
     4261 taaaacctag tagtaaaggg acaattatta acttgttgat tgtaagaaca taaatacttg
     4321 tagttttaaa agttgatttc aagttcatgc cggccagcag cgctccaata taaaccatgg
     4381 agagcgggct ggtacatttg ccaagactcg aaattggctc taggataaaa gcaggtagtt
     4441 taacctttaa caccatgagc aggatgccga gcagcagggc aattgtgttt atattaaaaa
     4501 aattacccaa tttggatttt tgaactcccg cacttagctt ataggttcca taggtatagg
     4561 taataaaatt ggaagccagc tggtaaacgg aagcataaaa aactcctacc ccgttaggaa
     4621 agagggcatc aaataaagga aaacccagaa aaacaatatt tccaaacatg gtatgaagaa
     4681 catgcacggt tgagtcagat ggagataact tttgtgctct ggcagacaat gtaccagcca
     4741 tgtacaggac accaatattt acaaaagtca gaacaaatag ccataaaccg tttacaagaa
     4801 gtcgggggtt gctttcgagt ttgagaaagg tggaaacaat caataaggga agagtaacat
     4861 caataattaa ttttgacagg aaatcttttc cctcgtcatt aataattttt ctccacgagg
     4921 caattgctcc tattacagct aaaactataa aggcaaggag ttgattaatt ataatattgg
     4981 attccattcc ataattttct ggttaggcaa aagtatttaa aaagtgggat tctctcgttt
     5041 ggaattattg ggaaattagg gaagggtcat ccatgttgaa tattctataa agcgttaggt
     5101 tgatgacaag acaggaaaca ctcctgcaag tcaacttgca caaacccgca ggaacatccg
     5161 gtttttaacg caagtttgta gaagctaaaa tggctgttca attttcttaa ccccgatgtt
     5221 taaagcattc gaacaaatgt atttgtaacc aaaaaccaat ttttgtacag aaaaaatact
     5281 acaaattttc tggtgtattt tcatcctctt cctgttccca gctccagact tttctgggtg
     5341 gtccctgatt tcgaaataat attgcagcaa ttagaccgct aataccgcca aaaagatgat
     5401 actcccacga aattgctgga taaaccggaa gaattcccca tattaaccca ccataaaaaa
     5461 acaccacaat caacgaaatt gccataagtc tttgatcatg ccttaccaca ccactaaaga
     5521 aaagaaacga ggccaatcca taaatgagac cgctagcccc aatgtggtag ctgtccctgg
     5581 ctccaaccca aaccattagc ccacttgtaa tccaaataaa caatattacc ttatatgata
     5641 ggtctctata aaaatatagt aatccaataa gtaagaataa aagcgataac gaattactta
     5701 tcagatggtt gaaatctgaa tggattaatg gtgaaaaaat tattccaggc agtccgctta
     5761 tttgtctagg aaaaacaccc agcctataga gtgaaatacc ctcaactatt tcaaagattt
     5821 taatgcacca aagcgctaca atgaaaataa gcgatacaaa aaggctgatg caaaaacgtt
     5881 tgcgttcgat gctcctgttt tcgttcatgt tgatggtgtg ggtattggat attttaagtt
     5941 gtagggatgt tgtatcgggg attttcaaca gtccgaaaat tttcttaaat aatatttggg
     6001 ctctatacct gaaatcccca aacaagcaca tcgtcgagtt gcggggttct attcatccac
     6061 tgcaaaaatt tcttttcgat tatatctacc ttaacattac ccttcatatt aggactatcc
     6121 gcaactatct gcatgaattg ctttcgcgtg aattttttac gtgattcgcc accaagttgg
     6181 tcaaaaaaac catcggagga taagtaaatc cagtccccat gattaagctg aatgttttga
     6241 agtgaaaagc ccgatgctga gtggctaaag tttgtgccaa gggtaataga gtcgggtttt
     6301 acttcttcaa cggaattccc cttttggata aaaataggaa taagcgctcc agaatagctc
     6361 agtaaaagcg attcggtatc aaaagtacaa acagctatgt ccatgctgtt tttcagaacc
     6421 tgctctcctg ttgacttatt taatctatta atgagagtac gatttatgaa agttagtatt
     6481 ttatcaggag agtaaaggtg ttgttcgtta atggcttgat tcagaatgtt aatacctatt
     6541 gtgctcataa aagcacccgg gacgccatgt ccagtgcagt cggctagagc cagaaaaacc
     6601 aggttatccc ttcgggttaa ccagcaaaaa tctccactaa caatatcttt aggcttgctg
     6661 taaataaacg catccaagaa aatggatagc atatcgctca agctgggttg aattgactca
     6721 tgtatttttt ctgcatacct aatgctatct gtaatatttt cgatgtagct ctcaataatc
     6781 ttatgcgcct cttcaagatt tttatttttt tgctcgataa aggcctggtt gctttctatt
     6841 ttaatttttt gttgttcaat ttcttccttt tgaatttcaa ggagccgatt tttatcctca
     6901 atctccttaa tttgatcgga tagaacaatg tttgatttta cctttgttct ccatgcaaac
     6961 agagaaacaa taacaactaa agagcttagt acaagtccaa taatgagagc atttttctgt
     7021 gcgttattct tttcaagttc tttttgcttt agtgcctgtt cttgcagcaa gtagctaatt
     7081 tccttttcct ttttttcggt ttcgtaaatt gtctgtattg ttgaaagatg tcgtgcattt
     7141 ttctcgttta aaaggctgtc tttgaaccgt gaagcgttct tatagctaag taacgccgat
     7201 aaataatctg atttttgaac aaagtaatcc gagtatgctt cataggcaga ctggagatgc
     7261 caagggcttc ctatttgttg ggctatggta attgtgctgt ccaggtagat ttttgccttg
     7321 tcaatttttt ttagggccag acaggcttta gccaggttta cgtagctatc ggcaagaaac
     7381 tttttatcac ctaattttct atctatttca agagcgtttt ggaaaagtct gtacgcattt
     7441 tcgggattac cattaaacat ttctatttta ccaagcgcat tatagttgat tgagattcct
     7501 tttagatttt ttagcttgaa attgatgtcc aacgacttgg tgtagtagta cttggcatta
     7561 ctatagtcgc ccataaattc ataaacttca cctatattgt tgaagttcat cgcttggcca
     7621 agtaggttat tctggctaat agatagggcc agcgctctgt taaaatacga aagtgcctgc
     7681 tggtaacgtc cattaagtga aaaaatattt cctaagctat tacaggcaac ggaaatattg
     7741 aatgtatcct tacatccctc ggcaatatta agtgccctta gatggtattc agatgcttgg
     7801 gcatggttgt cgagtcttcg atagacaaca cctatgttgt ttaatgttgt cgcctgaaga
     7861 atacaatcac ccatcttaac ggctaaagag tcagcaagat gatggtattt gagtgcttcc
     7921 ggatattttg actgatttct ataaaacttg cccagctcgt tataggtgtt ggctaaaaga
     7981 gtattgtctc cagctgtctg ggctatctgt aatgcctcgt atagataatt ttcggcctgt
     8041 atgaaatttc tgtcatgctg atcgtaaaaa gatgatatgt ttagtaagat ttcgaccctc
     8101 gacctatcat ttgactcctg gtacagtttt agcagcaggg aatcgatatt ttgtgatatg
     8161 cctaccctga aggcaaataa taaacagaga gaaactatcc gaaatttcat aaatcttaat
     8221 tttgctttcc tggcaaatgc ttcacgttga tattcaccta aaagaaacag gtaaatttat
     8281 atattttaat cgatctacaa agcacataat aatttcttat aaccttttaa cagtttcaga
     8341 cgaccgaaaa atataccact acctaaacta ttaatttatc ggaatgtgct tattcgttat
     8401 cttaaaggta tttatactta attactagtc gtatagaaaa aatatgggta tttaccagca
     8461 aaaaaataaa tttgtggatt tttaatgggc gtgtaaactg gtgctaaagc ctttcccttt
     8521 gcttgcatct cccaacatgt atttaacatt tagggatttt gccaaaatgt ataggattag
     8581 gaagtattta taa
//
LOCUS       DAOOQX010000004         8575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_10193_length_8575_cov_3.739202, whole genome shotgun sequence.
ACCESSION   DAOOQX010000004 DAOOQX010000000
VERSION     DAOOQX010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10193_length_8575_cov_3.739202"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /gene="pdxH"
                     /locus_tag="PLX53_00130"
     CDS             <1..428
                     /gene="pdxH"
                     /locus_tag="PLX53_00130"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOY71517.1"
                     /translation="GIAFFTNYESRKARNLLKNPYAAMVFFWPELERQVRIEGKVSRV
                     SEQQSDRYFQTRPEGSKIGAWASPQSSVIPNRRYLEQLHADFMEEFQGTTIKRPPNWG
                     GYILQPALFEFWQGRENRLHDRIQYSLVNEKWLIERLAP"
     gene            918..2078
                     /locus_tag="PLX53_00135"
     CDS             918..2078
                     /locus_tag="PLX53_00135"
                     /inference="COORDINATES: protein motif:HMM:NF040309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HOY71518.1"
                     /translation="MHDELEIVEYNFESSSHKILPGVNKYHCTLHNLDIECNYTYSTE
                     VGNPNSNVDTKDICIEENRFYKYQIIKPKGEATTQRVVFLFHGFNEKDWGKYVPWAKA
                     ISESTGFSVILFPIAFHMQRAPKGWSNKREMFKLSEMRKRSYPNVVHSTLSNVAISIR
                     LHSMPQRFIWSGLQTYYDVIQLIEECKEGKHPYISKNFEFDFFAYSIGGFLAQILKLS
                     NYNNYFANSKVCLFCSGATFNRLSPVSKFILDSEANIALYSFLIEHFDSVLQKDNLLH
                     HYITEHLEGQVFRSMLNYQRMRDLREGLLKKYEDQIYAITLKKDLVIPSFEVINTLKG
                     AYRTINIKVEELDFNREYIHENPFNADSSASQLVDEDFKLVFSKVSDFFKKG"
     gene            complement(2381..6196)
                     /locus_tag="PLX53_00140"
     CDS             complement(2381..6196)
                     /locus_tag="PLX53_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOY71519.1"
                     /translation="MASRIVLLVTNKVLPSFQFEPRLDGMGYSLITLDRIGSDVELPA
                     FDLAIVGVADRASYNAILKVFRKYPEKPLLFYSGKNTADAQKSIPELDSTNHVNPDFT
                     NHELSLQVELAITRKWFDHSSIFSDGNAGSNHVRLKKILSEEQAWRVLFEQSPNGVLL
                     SDSAGSIIFTNNATSEILGYSSSQLLKMRFHDLVPPETLPQVDQNIKKILAGEQLITE
                     VYNLRKDGSKRFVQLHESRVLFPSGEFGIMVVSIDMTRAKKAEDALGESLEKYRILVE
                     QSNDGIIYSKDGIITYGNPRIAELLEVQHCELVGKPFYTYLPKSEWEKLQNRYKNRLE
                     GIKEPSIYETLLVSAHGSNIPVELNVNLATIGNERVTMVVVRDISLRKEAEKAIRESE
                     ESYRGLFNNSGEAIYIIDASGILLDVNPSGLKLFGKEKEETLGLTLMELADQEKSNTE
                     EVKRKIRIAHQGQTQYLEFWGKKSNGDRVPMDIVLSKGTYFGLQVVLAIGHDITERKN
                     AEAILRESEDKFRTLTEQIPVGIYRLTPEGKILYGNPAFAKTLGFNDTSEVIGHSMDE
                     FIATNYDDWVEESVKLSPGAPAEQSFRRQDGINLWANNQLRRVKNASGEVDYLDGVLV
                     DITEHKFTLDALKESEERFRAMVTAIPDRLFRINADGDLIDFAPSEQLLDFELNAGMI
                     GHSLSEFLPQDVFQKFTDAISLAKQSGKLQTFEYKFSKNNQDYFNEVRLLQSGRSLFL
                     VLQRDITQKKQFEAQANMLAQTIMTANDSISITNTDGHFIFVNPAFTKTYGYTQSEII
                     GKHTSILKTPDRDLTLDKTIHEETLKGGWQGELINVRKDGAVFPIFLSTSAVYDENGI
                     AIAHVGIANDITERKKIEQELIRAKEKAEESDRLKTAFLSNMSHEIRSPMNAVLGFTQ
                     LIKSDEQLSETGMQYIELIQNSGKQLLSLLEDIIDISKIQSNQLRIDKNAFDLNELMD
                     ELYLIFSNQLKLKDECKTLLFKPEKTCVSPFVIYSDPVRIKQILSNLLSNAIKFTPSG
                     SINFGYSIFSDDDASKIQFYVRDTGIGISIENQLLIFERFRQADNSYTRLYGGSGLGL
                     AISRGLVELLGGDIWVESKPGEGSEFFFNIPLEFSEKDTPAPVQPSSEVKQPVSQLKG
                     KTVLVVEDVNEIRLYFERVLERTGAKVLFAKNAKEARKLFKSNNRIRLVLLDIGLSDA
                     DGFELAVEFKRMRPNTPIIAQTAYALQSELNRSIESGCDDYITKPLDSAVLIGKMLRL
                     IGSKSKQSD"
     gene            complement(6456..7520)
                     /gene="arsB"
                     /locus_tag="PLX53_00145"
     CDS             complement(6456..7520)
                     /gene="arsB"
                     /locus_tag="PLX53_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOY71520.1"
                     /translation="MPSKSRLKFFDRYLTLWIFLAMLVGVLSGYLFPSIAVFWDSLKA
                     SPESTTNIPIAIGLILMMYPPLAKVKYEELGDVFRNTRVLSLSLIQNWVIGPILMFLL
                     AIVFLRFNIDYMYGLILIGLARCIAMVIVWNELAGGDTEYAAGLVAFNSVFQVLFFSV
                     YAYLFIAILPGWFGLPVNETISQITIGQIATSVFIYLGIPFIAGFLTRLVLTRIKSKA
                     WYNTHFIPKISPLTPIALLFTILVMFSLKGEYIVKIPLDVVRIAIPLMIYFVVMFLIT
                     FFMSRRIGASYRQTATLSFTAASNNFELAIAVAIAVFGINSGEAFAAVIGPLVEVPVL
                     ISLVNVSLKLKKKYFSQQAV"
     gene            complement(7525..7944)
                     /locus_tag="PLX53_00150"
     CDS             complement(7525..7944)
                     /locus_tag="PLX53_00150"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOY71521.1"
                     /translation="MKILILCTGNSCRSQMAEGFLKLFGGNFEVHSAGTQPAPAVNSY
                     AIKVMGEVGIDISHNRTHHVDEFLNEEWDYVITVCGGARESCPAFIGKVKNRLHIGFE
                     DPAEAQGSDEEVLTVFRRVRDQIGEEFRKFHDNLLKP"
     gene            complement(8038..>8575)
                     /locus_tag="PLX53_00155"
     CDS             complement(8038..>8575)
                     /locus_tag="PLX53_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71522.1"
                     /translation="RKTTHQFINYKGWVNEEITLKYNDTTGYLNEINKSVESKIINTA
                     LVSCDSTGRIVEVRVFNEKGELSGIDRLIYVPANNLIRVLSYKANDQFVMATAYPLDP
                     DKPEPKSTLKREFNSHGDVILELLPGSKIGQGYYYEYSYDAFGNWTEKLTYQCTVTPN
                     GNVKDKKLEHRITRKIVY"
BASE COUNT         2261 a         1912 c         1922 g         2480 t
ORIGIN      
        1 atggaattgc tttttttacg aactacgaga gccgcaaagc caggaacctg ctgaaaaacc
       61 cctacgctgc tatggtgttt ttctggccgg agcttgaaag gcaggtaaga attgagggaa
      121 aggtgtccag ggtgagcgag cagcagagcg accgctactt tcaaactcgt ccagagggaa
      181 gcaagattgg tgcatgggca tcgccgcaaa gttcagtaat tccgaacaga aggtacctgg
      241 agcagcttca tgctgatttc atggaggagt ttcaaggaac aaccattaag cgtccaccaa
      301 attggggtgg ctacattctt caacccgctc tttttgagtt ttggcaggga agagagaatc
      361 gcctacacga ccgcatccag tactccctgg ttaacgagaa gtggttgatt gagcgcttgg
      421 ctccctagcc gagccgttgg cttttccacc agtcaatgta aacattacta ttatccagcc
      481 cagccagacc tggcggttcg gagatattgc ttttgcaccc acctatgcta aattttgtgc
      541 gctttggcaa gttgcattca tgcgccaatg cccccggtta cttttttgta gggcttaaaa
      601 tggcaaagcc ttaaaacttc aaccttaagt gtgaattctg ccacgaaggc ttgagatagt
      661 gttaggatat aagctacctt tgaagcaggt tatctgcatt tgggcgggta agcgtactgt
      721 tggataatgc ccgaagccgc tggcagtgtg aacctgaaat gagacatcct cattaccggc
      781 tatgatgtgt ggtgagtact ttccctggat tactaccttt ttgccaaccc ctagcagaga
      841 ggaacaaata tttttttaaa actatacacc tagggacata gtaaagtgtt ttgctagttg
      901 taataaaaag ttgaaatatg catgatgagc tggaaatagt ggagtacaac tttgagtcgt
      961 catcacacaa gattcttcca ggtgtgaata aatatcattg cacattgcat aacctagata
     1021 ttgagtgcaa ctatacttac tctaccgagg ttggcaatcc taatagcaat gttgacacca
     1081 aggatatttg tattgaagaa aacagatttt ataagtatca aatcataaaa cctaaaggcg
     1141 aagccaccac ccaaagagtg gtttttctat ttcatgggtt caacgagaaa gactggggaa
     1201 aatatgtgcc ctgggccaag gcaatttctg aaagcacagg attcagcgtt atactatttc
     1261 ctatagcttt tcatatgcag cgggcgccaa aaggctggag caataaaagg gagatgttta
     1321 agctgagcga aatgaggaaa agaagctacc cgaatgtggt gcactcaacc ctgtctaatg
     1381 ttgccattag catccggctg cattcaatgc ctcagcgatt tatctggtcc ggattgcaaa
     1441 cttactatga tgtaattcag ctgattgaag aatgcaagga aggaaagcac ccctacatca
     1501 gcaaaaattt tgaatttgat ttttttgcct actccatagg cggttttctt gcccaaatac
     1561 ttaagctaag caactacaac aattattttg ctaactcaaa ggtttgttta ttttgcagcg
     1621 gagccacttt taaccggcta tcaccagttt caaaatttat actcgatagt gaggccaaca
     1681 tagcgttata ctcattttta atcgaacatt ttgattccgt tctgcaaaaa gacaacctgc
     1741 tccatcatta tatcactgaa catttagagg gtcaggtgtt tcggtcaatg ctcaactacc
     1801 agaggatgag agatctccgt gaaggcttat tgaaaaaata cgaagatcag atttatgcta
     1861 tcaccctaaa aaaagatctt gttattccct cttttgaggt aattaataca ctaaaaggag
     1921 cctaccgcac aattaatatt aaagtagaag aacttgattt taacagggag tatattcatg
     1981 aaaacccttt taatgctgat agtagcgcat cacagctggt tgatgaggat tttaagctgg
     2041 tttttagtaa agtttccgat ttttttaaaa aaggataact tcaaaatttg gcttgattta
     2101 ctttctatct ctggtaaaat gtgccccaaa atggacgcta ttagattctt tgccgtatcc
     2161 gttcttcagc caaagttttt ctacgaaaag attcatttcc cattagcctt aatgaaactt
     2221 aagacatcac gccataattg gcctacaaaa tatgttaggt tcagctgtta agttggatta
     2281 ccggacaagt tagggtttgg ctgtgtttcc atccctgaag ccctgccaga tgggggtatt
     2341 tttccatcca ttcccttgct acggttgaac gcttagtccc ctaatcggac tgcttggatt
     2401 tgctaccaat caatctaagc attttaccaa taagcacagc ggaatctaaa ggtttggtga
     2461 tgtagtcgtc gcaaccgctt tcaatgctcc tgtttagctc cgactgcagg gcgtaggccg
     2521 tttgggcaat aatgggcgtg ttgggccgca ttcgcttgaa ctcaacagca agttcaaaac
     2581 cgtcggcatc ggaaaggcca atgtctagca gaacaagccg gatgcggttg tttgacttga
     2641 atagcttgcg tgcctccttg gcatttttgg caaatagaac ttttgcaccc gttcgttcca
     2701 gcacccgttc aaagtaaagc ctgatttcgt taacatcctc aacaacgagt acagtttttc
     2761 cttttagctg gcttacaggt tgtttcactt cgcttgaagg ttggactggg gctggcgtat
     2821 ctttttcgga aaattcaagg ggaatgttaa agaaaaactc tgaaccttca ccaggtttac
     2881 tctccaccca gatatccccg cccaggagtt caacaaggcc tcttgaaatt gccaggccaa
     2941 gacccgaacc cccatataat cgggtgtatg agttgtcggc ctgcctaaaa cgctcaaaga
     3001 tgagaagctg attttctata gagatgccta ttcctgtgtc cctcacgtaa aactgaattt
     3061 ttgaagcgtc atcatccgaa aatatggagt atccaaagtt tatacttccg cttggggtaa
     3121 acttaatggc gttgctcagc aggttggaga gaatttgctt aattctcacc gggtcggagt
     3181 atataacaaa aggagataca caggtttttt cgggcttgaa gagcagggtt ttgcactcat
     3241 ccttaagctt aagctggttg gaaaatatca ggtaaagctc atccatcagc tcgttcaggt
     3301 cgaatgcgtt tttatcaatc cgtagctggt tcgactggat tttagaaata tcaataatat
     3361 cctccagcag cgacaggagc tgcttgccgc tgttctgaat cagctcgatg tactgcattc
     3421 cggtttccga tagctgctca tcacttttga ttagctgggt gaaccccagc acagcgttca
     3481 ttggcgacct gatctcatgg ctcatgttgg agagaaaagc tgttttgagt ctgtccgatt
     3541 cctctgcctt ttcctttgct cggatgagct cctgctcaat cttttttcgc tcggtaatgt
     3601 cgttagcaat tcccacatgg gctattgcga ttccgttctc atcgtaaacg gccgaggtgg
     3661 aaaggaatat tgggaaaact gcgccgtctt ttcgaacgtt gatgagctct ccttgccatc
     3721 cgcccttaag ggtttcctca tgaattgtct tatcaagtgt taagtccctg tcgggggttt
     3781 tgaggatgga ggtgtgcttg cctataattt cggattgggt gtagccatag gttttggtga
     3841 aggcggggtt aacgaaaata aaatgtccat ctgtattggt aatggagatg ctgtcgttgg
     3901 cggtcatgat ggtttgcgcc agcatgtttg cttgagcttc aaactgtttt ttctgggtaa
     3961 tgtccctctg taggaccagg aaaaggcttc gtcccgattg aagtagccta acttcgttga
     4021 agtaatcctg attgtttttg gaaaacttat actcaaaagt ctgaagcttg ccgctttgct
     4081 ttgccaggct aatggcatca gtaaattttt ggaaaacgtc ctggggtaga aattcggaaa
     4141 gggaatgccc tatcattcca gcgttaagtt caaaatcaag cagttgttcc gaaggggcaa
     4201 aatcgataag gtcaccgtcg gcattgattc ggaacagcct atcgggtata gctgtaacca
     4261 tggcccgaaa acgctcctcg ctttccttta gagcatccag cgtgaattta tgctcggtga
     4321 tgtcaaccag caccccatcg aggtagtcaa cctcgccgga agcatttttg acccttcgga
     4381 gttgattgtt agcccataaa tttatgccat cttgtctgcg gaacgattgt tcggcaggtg
     4441 cacccggtga tagctttact gattcttcaa cccaatcgtc gtaattagta gctataaact
     4501 catccatgct gtggcctatc acttcgctgg tatcgttaaa accaagcgtt ttggcaaagg
     4561 cgggatttcc gtacagtatt ttcccttcag gggtcaaccg gtaaatgcca acaggtattt
     4621 gctcggttag ggttcggaac ttatcctcac tttcacgcag gatagcctca gcattttttc
     4681 gctcggttat atcgtgtcca attgccagca caacctgaag cccaaagtag gtgcccttgc
     4741 taagcactat atccattggc actcgatcgc cattgctttt ctttccccaa aattctaagt
     4801 attgagtttg accctgatgc gctatcctaa tcttccgctt cacctcttcc gtgttgctct
     4861 tttcctgatc ggccagctcc attagggtta agcctagggt ttcctccttt tctttaccga
     4921 acagcttaag cccgcttggg tttacatcaa gcagtatgcc gctggcgtca atgatgtaaa
     4981 tggcctcacc ggagttgttg aaaagccccc tgtagctctc ctcgctttct cttatggcct
     5041 tttcggcctc tttccgtagc gaaatatccc taacgaccac catggtaacc ctttcgttgc
     5101 caatagtggc caggtttacg tttagctcaa caggaatatt gctaccatga gcactcacaa
     5161 gtaaggtttc atagattgaa ggttctttta tcccctccaa ccgattttta taacggtttt
     5221 gtaatttttc ccattcgctt ttaggtaggt aggtataaaa tggcttgcca actagctcgc
     5281 aatgttgaac ctccagcagt tctgcaatgc gtgggtttcc gtaggttatt atgccatcct
     5341 ttgagtaaat tatgccatcg ttggattgtt ctaccagaat tctgtacttc tccagcgact
     5401 cccccagcgc atcttctgcc ttttttgctc ttgtcatgtc gatggacaca accataatgc
     5461 cgaattcgcc gctgggaaat aaaactcggg attcgtgcag ctgaacaaat cgcttgctgc
     5521 catccttgcg taggttgtaa acctcggtga taagctgctc accagcaagt atttttttta
     5581 tattctgatc aacttgagga agcgtttctg gcggaacaag gtcgtgaaac ctcattttaa
     5641 gtaattggga ggacgaatac cccagaattt cgcttgttgc attattcgta aaaattatac
     5701 tcccggcgct gtcgctaagc agaactccgt ttggcgactg ttcaaaaagt acgcgccagg
     5761 cttgttcctc agaaaggatt tttttaagcc taacatgatt ggatcctgca ttgccatcgg
     5821 agaagatgga ggaatgatcg aaccacttcc ttgtgatggc cagttctact tgcaaactca
     5881 gctcatggtt ggtaaaatca ggatttacgt ggttggttga gtctagctcg ggaatagatt
     5941 tttgagcatc ggcagtattt ttgccggagt agaacagcag tggtttttcc gggtatttcc
     6001 taaaaacttt aaggatggcg ttgtagcttg cacgatcagc tactcctact atggccaagt
     6061 cgaatgccgg tagctccaca tccgagccaa ttctatcaag ggtaataagg gagtaaccca
     6121 tcccatcaag ccgaggctca aattggaaag aaggtaaaac tttgttggtt accagcagca
     6181 ctattcttga agccataacc aaaagtttga aagttgaaat ttagcatttt tcaactccga
     6241 tttcaacttt ttttaacata acatccgtca acccaaggta tattttgacg atggagatta
     6301 atttgtttac cagcaacccg ttacagtctt tagatggaaa aggtttgcag ctgctatatc
     6361 aaaagtgagc cgttttgggt tggtagctct ccatggcatt tagaaatgat aaattttgat
     6421 acacaacctt cctataaagc catacctgat attcatcaaa cagcctgttg gctaaaatac
     6481 tttttcttaa gctttagaga cacgttaacc agtgaaatca acacgggtac ctcaaccagc
     6541 gggccaataa ccgcggcaaa cgcctcgccc gagttgattc caaacacagc aatagccacc
     6601 gcaattgcaa gttcaaagtt gttgctggca gcagtgaacg acagggttgc cgtttgacgg
     6661 tagctggcac caattcgcct gctcatgaag aaggtaatca gaaacattac aacgaagtat
     6721 atcatcagcg ggatggcaat tcttaccaca tctaaaggaa ttttaacgat gtattcaccc
     6781 ttgagcgaga acatcaccag gatggtgaac agcagggcaa tgggtgttag ggggctaatt
     6841 ttcggaataa agtgggtgtt gtaccaagct ttacttttaa tgcgggtgag aacgagtcgt
     6901 gttaaaaacc cggcgatgaa gggtattccc aggtaaatga acacgctagt ggctatttgt
     6961 ccgatggtaa tttgtgaaat ggtttcgttc accggaagcc cgaaccagcc tggcagtatg
     7021 gcaataaaca ggtatgcgta aaccgagaag aacagcacct ggaaaaccga gttgaaggct
     7081 acaagcccgg cagcatactc agtgtcgcct ccggctagct cgttccatac aatcaccatg
     7141 gcaatgcatc ttgcgagccc tatgaggatg aggccataca tatagtcaat gttgaatcga
     7201 aggaatacaa tggccagcag gaacatcagg atgggcccaa taacccagtt ttgaattaac
     7261 gatagggata aaaccctggt gttgcggaaa acatcgccca gctcctcata cttaaccttg
     7321 gccaagggtg gatacatcat tagtataagc ccaatggcta ttgggatatt ggtagtgctt
     7381 tcgggcgatg cttttagtga atcccaaaat actgctatgg atgggaaaag gtagcctgac
     7441 agcacaccaa ccagcatggc caggaaaatc cacaacgtca ggtagcggtc aaagaacttt
     7501 agtcggcttt tcgaaggcat ggctttaagg ctttaacagg ttatcgtgaa atttccggaa
     7561 ttcttcccca atttgatctc tcacccttcg gaataccgtg agcacctcct catcgctgcc
     7621 ctgtgcttcg gcagggtctt caaaaccaat atgaagccga tttttgacct tcccaatgaa
     7681 tgcggggcag gattctctgg ctcctccaca aacggtaatc acgtagtccc attcctcgtt
     7741 aagaaactca tctacatgat gggttcggtt atggctgatg tcaattccca cctcccccat
     7801 tacctttatg gcatatgaat ttacggctgg tgcgggttgt gttccagccg aatgcacttc
     7861 aaaattacct ccaaacaact tgagaaaacc ctccgccatt tggctacggc agctgttgcc
     7921 ggtgcataga attaatattt tcatggttat tcccgatata agaatggatg gagtgcgtct
     7981 aattccgtaa cctcaaaaaa attatactct aaaaaaacac taaaaaatgg gaaaagttta
     8041 gtacactatt ttccgggtaa ttctatgctc caactttttg tctttaacat tcccgttggg
     8101 ggtaaccgta cactggtagg ttagcttttc agtccagttc ccaaaagcat catagctata
     8161 ctcgtagtag tagccttgtc caatttttga tccaggcaac agctcgagaa tgacgtcgcc
     8221 atgggagtta aattctcttt tcagggttga tttaggctcg ggtttgtcag gatcgagggg
     8281 gtatgcggtt gccattacaa actggtcgtt ggccttgtag gagagcaccc tgataaggtt
     8341 gttggcaggc acatagatga ggcggtctat accgctcagc tcgccctttt cattgaatac
     8401 cctaacctcc acaatccgtc cggtgctgtc gcagcttacc agagccgtgt ttataatctt
     8461 actttcaaca cttttgttaa tctcgttcag gtatccggtg gtgtcgttat atttcagggt
     8521 gatttcctcg ttcacccaac ccttgtagtt gatgaattga tgcgtagtct ttcta
//
LOCUS       DAOOQX010000005         8280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_10651_length_8280_cov_2.818237, whole genome shotgun sequence.
ACCESSION   DAOOQX010000005 DAOOQX010000000
VERSION     DAOOQX010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10651_length_8280_cov_2.818237"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLX53_00160"
     CDS             <1..273
                     /locus_tag="PLX53_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOY71523.1"
                     /translation="LDCIPVINQGAVTIDQPSLLVCYTDGLVEIMRNNKVEYNTQVIE
                     QEIVKLEPISTTIQRIAESRGIKNDGSSNPEIFDDISILGFGFNCC"
     gene            complement(260..1699)
                     /locus_tag="PLX53_00165"
     CDS             complement(260..1699)
                     /locus_tag="PLX53_00165"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOY71524.1"
                     /translation="MVTDEHKTKLAALFLGLAFVAINSLLVTKNILSFSLLPVILLLV
                     LGVVLVPVKIFYLVIALAPLSVPFSEFLPGRSFDLWLPTEPLIFALLLLTAIHLIQER
                     RFDTGLFGNPLFWAMLFYLGWLVITCISSELPLVSVKYTVLRIGYIAFFFYIPFVLFK
                     ATPQNFTKYIAFYITGLTVVVAITLYRQARIGIFDKFAAVGTCNPFYTDHTSYGAALA
                     FLIPVVVAWAASTKTLWQKILMGILILVFTVALVLSYSRAAWLSLLVAVLIWVVWLMK
                     LRFRTIAILSVSIVAIGFGLRGQLAQWVGKNQTVSTGSLSDQLLSVGNITTDVSNVER
                     LNRWTSALRMFRERPFLGWGPGTYQFTYAPFQPSQFFTVESSNLGAKGNAHSEYLGLL
                     AEAGWPATMGYIAILLVAFIMGFRLLGKLPLDFPYRKLLLGVLLGLSTYAFHGFLNNF
                     LDMDKIAALFWGYLAMIAAMNTAYRTSSS"
     gene            complement(1710..2123)
                     /locus_tag="PLX53_00170"
     CDS             complement(1710..2123)
                     /locus_tag="PLX53_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71525.1"
                     /translation="MAAAVANAAVGIADSMMRSLKSEVAAKTLLALEHQHRLALMELA
                     SLQDSFANLKKSFGLAPAVELSGLRQAIAFARVQVTGDIPTCYVIDRAVKPSVKVSPN
                     RGLIVVISTLSAIFFTIFVVAVLELARGSKLNQGD"
     gene            complement(2183..2575)
                     /locus_tag="PLX53_00175"
     CDS             complement(2183..2575)
                     /locus_tag="PLX53_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71526.1"
                     /translation="MEQDYRTAEPSFVATVKVWKRYRLVFLIVGVAALVGSTVASLLI
                     APKFRATAVFAPTRELQVPGTPLSDSLFVEFNNFGGAVEREQFLEVMASGAFRDTLIS
                     RMGLHSYWGLQTTGDKSMQAARNPMASM"
     gene            2706..2945
                     /locus_tag="PLX53_00180"
     CDS             2706..2945
                     /locus_tag="PLX53_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71527.1"
                     /translation="MKNLNFTRGQVTNILEGIAKKEEGVQHILKMSIEAIKMVWRDVY
                     NKRHQDISNSMKMQILPLFLHLALGNTTYGREGAI"
     gene            complement(3123..4415)
                     /gene="tyrS"
                     /locus_tag="PLX53_00185"
     CDS             complement(3123..4415)
                     /gene="tyrS"
                     /locus_tag="PLX53_00185"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOY71528.1"
                     /translation="MNFIDELQWRGLVHDVMPGTADQLNKEMTSGYIGFDPTAESLHV
                     GSFAQIMLLKRFQLCGHKPIVLVGGATGMIGDPSGKSQERNLLDSATLQKNLEGIRNQ
                     LSRFLDFDSGNPNAAVMVNNYDWMKNFSFLDFIRDVGKHITVNYMMAKDSVKKRFGEE
                     TKEGMSFTEFSYQLVQAYDYLWLYKNLGCRLQMGGSDQWGNITTGVELIRRMTGGEAF
                     ALTIPLITKADGSKFGKTESGNVWLDSKLTSPYKFYQFWLNTSDDDAEKYIKVFTLLD
                     QSTVDALVEEHRREPHLRKLQKMLAREITIMVHSEEDYRKAVEASELLFGNATTETLM
                     SMDEDTFLSVFEGVPLFEVSRQHIAEGVPLSTLAVELAPIFSSKGELRRLVTGGGLSL
                     NKVRVENTELPVSNSHLLNGKYILIQKGKKSYSIIRTV"
     gene            complement(4530..5060)
                     /locus_tag="PLX53_00190"
     CDS             complement(4530..5060)
                     /locus_tag="PLX53_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOY71529.1"
                     /translation="MAVFDIVVVILLVVGAIRGLRQGFLSQLASVVALLLGIWGAVRF
                     SDYMATLLSEKFDIGSAHLPIISFAVTFILIVIAIHFIEKIVERFLNLAMLGGINKVL
                     GFVFGFAKYAIITSVLLVFVEKANIRFNFFTEEQKSKSLVYNPVASLAPAIFPYLHFD
                     TIKESVKKSLGNQKGK"
     gene            complement(5078..5386)
                     /locus_tag="PLX53_00195"
     CDS             complement(5078..5386)
                     /locus_tag="PLX53_00195"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOY71530.1"
                     /translation="MVKINELKVEQLEDAANMLKAMAHPMRIAIISLLEANGRLTVTQ
                     IHEKLQIEQSTASHHLGILKDKGLLACKREGKNNLYFLRNENLGKILDCVGSCTSTRF
                     "
     gene            5920..6888
                     /locus_tag="PLX53_00200"
     CDS             5920..6888
                     /locus_tag="PLX53_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOY71531.1"
                     /translation="MLEAIKQPVDEELKKFEPFFRKNMGSNIRFLDLITNYIYRNKGK
                     QLRPIIVFLSAGLNGKICQSTYVAAGMIELLHTATLIHDDVVDESFERRGAFSINALW
                     RSKVAVLVGDFLLSKGLLVAVQNKEYELLEVMSNAVREMSEGELLQIEHSRKMDINEE
                     AYYDIIRKKTAYLIASCCENGAISAGADSDSIRRMHEFGINLGMAFQIRDDLFDYQPK
                     GLVGKPNGNDIKEKKLTLPLIYALREAPAAEGRKVLREIRSRSKSSVTISTVVKFTNE
                     YGGISYATGQMLNFKQQAQEVLNSFPDSDYRQSLMLLTDYVTDRNK"
     gene            complement(6892..8214)
                     /gene="dgt"
                     /locus_tag="PLX53_00205"
     CDS             complement(6892..8214)
                     /gene="dgt"
                     /locus_tag="PLX53_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HOY71532.1"
                     /translation="MEWTKLLDASRFGATGATKNTYDTARTNYQHDFDRLIFSSPFRR
                     LQNKTQVFPLPGSIFVHNRLTHSLEVSSVGRSLGQNVYLQISETLSPHEKPLVAEIPG
                     IIAAACLAHDMGNPPFGHSGEEAIRKFFEDNSGVFKPMVDSWEWSDLVRFEGNANALR
                     LLTQQLTGRRTGGFRLSNAVLASVVKYPYPSASGKKKFGFFKSEANPFLSIAHATGMH
                     SVGNMEFCRHPLVYLVEAADDICYQIMDIEDAHKLGILSTPMVVDLYRAFFTDEPKSA
                     RDRITSTLKEVQDPNEQVAYLRAIVISKLVEECARIFVDNLKPIMDGEDFSSLLEKIS
                     GASHTAMEAIKNLAVERVYNHRKVVEIEIAGYQILGSLLEIFCDAVLHPQTGLSKKVI
                     RLVPNQYMTSSDDDYTKIMSVLDFVSGMTDVYALETYRIIKGINLPTL"
BASE COUNT         2294 a         2001 c         1916 g         2069 t
ORIGIN      
        1 cttgactgca ttccggttat taatcaggga gccgttacca ttgaccagcc gtccctgctg
       61 gtttgctaca ctgatggcct ggtggaaatt atgcgcaaca acaaagtgga gtacaacaca
      121 caggtaatcg agcaggaaat tgttaagctt gagcccatat caaccacaat tcagcgtata
      181 gccgagtcgc gtggcattaa gaacgacggc agcagcaacc ccgaaatttt tgatgacatt
      241 tcaattttag gctttgggtt caactgctgc tagtcctata cgctgtattc atagcagcta
      301 tcatagccag atatccccaa aaaagggctg ctattttgtc catatccaaa aagttgttca
      361 ggaacccgtg aaaagcatag gttgacagcc ccagcaacac cccgagcaat aacttccggt
      421 agggaaaatc cagcggcagc ttacccagca acctaaaccc cataataaaa gcaactaata
      481 gaattgcgat atatcccata gtggcaggcc agcccgcctc ggcaagcaaa cccaggtact
      541 cgctatgggc attccccttt gcccccaggt ttgaactttc aacggtaaaa aattgcgaag
      601 gctgaaatgg ggcataggtg aactggtatg taccaggccc ccaacccaaa aacggacgct
      661 ccctaaacat gcgcaatgcg ctggtccacc ggtttagccg ctcaacgttc gacacgtctg
      721 ttgtaatatt tccaacagaa agcagctgat cgcttaggct gcccgttgat accgtctggt
      781 ttttccccac ccattgggcc agctgacctc ttaacccaaa acctattgca acaatactca
      841 ccgaaaggat ggctatagta cgaaatcgca gcttcataag ccatactacc catataagta
      901 cagctactaa aaggctaagc catgccgccc ggctatagga tagcaccagc gcaacggtaa
      961 aaacgaggat aagtattccc attaatattt tctgccatag cgtttttgtg ctggcggccc
     1021 aagccaccac aacaggaatg agaaacgcca acgctgcacc gtaggaggtg tggtcggtgt
     1081 agaatggatt acaggttcca accgcagcaa acttatcaaa aatgcctatt ctagcctgcc
     1141 ggtatagggt tatagccaca actaccgtca atccggttat gtagaaggca atgtactttg
     1201 taaagttctg aggcgttgcc ttaaacagta caaatggaat gtagaagaaa aatgctatgt
     1261 aaccaattcg cagaacagtg tactttaccg ataccaaagg taactcgctg ctgatacatg
     1321 ttatgacaag ccatccaagg tagaagagca tggcccagaa gagtggatta ccgaaaaggc
     1381 cagtgtcaaa tcggcgctcc tgaataagat gaatagcggt taacagaagt agagcaaaaa
     1441 tgaggggctc ggttggcagc cacaggtcga aagaacggcc tggcaggaac tcgctaaaag
     1501 ggacggatag cggcgccagg gcaattacca ggtaaaatat ttttacgggg acaagcacta
     1561 ctcccagcac caacagcagg ataaccggta gcaggctgaa cgacagtatg tttttagtaa
     1621 ccagtaggct gtttatggct acaaaagcaa gaccgagaaa cagtgcagcc agctttgttt
     1681 tgtgctcatc ggttaccatg gccacgggat tagtcgcctt ggtttaactt tgaccctctt
     1741 gccagctcaa gaactgcaac cacaaaaatg gtaaaaaaaa ttgcagatag cgtggatatt
     1801 accactatca gccccctgtt tggggaaact tttacgctag gcttaacagc gcggtcaatt
     1861 acgtagcatg tgggtatatc acctgtgacc tgcacacggg caaacgcaat ggcctgcctt
     1921 aaccccgata actccacggc aggggctaac ccaaaacttt ttttgagatt agcgaaagaa
     1981 tcctgtaaac ttgccagctc catcaacgcc agccggtgct gatgttcaag cgccagaagg
     2041 gttttagctg ccacctccga ttttaaggag cgcatcatgc tgtctgctat accaacggct
     2101 gcattggcaa ctgctgcggc catttgcgga tcggcatcag taacctcaac cgctataccg
     2161 atgaattggg taggcttaac ccttacatgc ttgccatagg gttgcgggca gcctgcatgg
     2221 atttatcgcc tgttgtctgc aacccccagt agctgtgaag acccattcgg gagattagcg
     2281 tatccctaaa agcaccggag gccatcacct caagaaactg ctctctttca acggctcccc
     2341 caaagttgtt aaattcaacg aacaggctat ccgacaatgg agtgcctggt acctgtagct
     2401 cccttgtagg agcaaatacc gcagtagcac ggaacttcgg ggctattaac aaagaggcaa
     2461 cggttgagcc taccagtgct gctacgccaa caataagaaa taccaaacgg taacgcttcc
     2521 aaacctttac cgttgccacg aaacttggtt ctgcagttct ataatcctgc tccatgggtt
     2581 agggttgatg agaactcgaa aatttaaaat gctaaagttc catattcggc attaataata
     2641 gatactgtcc caaaaagtgt gtaaagccag gctttcataa ttttgaagtg taaactttta
     2701 aaaaaatgaa aaacctaaac tttacacgcg ggcaagttac gaacatttta gaaggaattg
     2761 ccaaaaagga agaaggcgta cagcatattt taaagatgag catagaggca attaaaatgg
     2821 tctggcggga tgtttacaac aaacggcacc aagatattag caattccatg aagatgcaaa
     2881 ttctgccatt gtttttgcac ttggctttag gcaataccac gtatggtagg gagggcgcca
     2941 tttaacccgt cgtaggttgg actaggcctg gaagatagca ccaatcaaaa atacccggct
     3001 tttcttcgct cttactatct ttgacaaatt cggagcatgc gctgcctttg cttaatcaag
     3061 cttacaggaa aaacgtccta acccgtttta agtaaagtgt caaaaaaata gggggttaaa
     3121 ccttataccg tacgtattat gctgtagctc tttttgccct tctgaatgag gatgtacttg
     3181 ccatttagca gatggctgtt ggaaaccgga agctccgtgt tttcaaccct taccttattc
     3241 aggctaagtc caccgccggt aaccagacgc cgtagttctc cttttgatga gaaaatgggt
     3301 gccaactcaa cggcaagcgt tgaaaggggc actccttcag caatatgctg ccggctaacc
     3361 tcaaataatg gcacaccctc aaaaacgcta aggaacgtat cctcatccat ggacatcagg
     3421 gtttcggtgg tggcattacc aaatagcaac tcggaggcct ccacggcctt tcggtaatcc
     3481 tcctcggagt gcaccataat ggtaatttcg cgtgctagca ttttttgcag ctttcgcaaa
     3541 tggggttccc tacggtgctc ctcaacaagg gcatcaacgg ttgactggtc gagcagggtg
     3601 aaaaccttta tgtacttttc ggcatcgtcg tcggaggtgt tgagccagaa ctggtagaac
     3661 ttatagggcg acgtaagttt tgagtcaagc cacacgttcc cgctttcggt ttttccaaac
     3721 ttgctcccgt cggccttggt aatcaagggg atggtaagtg caaaagcttc gccaccggtc
     3781 atccggcgga tgagctcaac ccccgtggtg atgttacccc actggtcgga accacccatc
     3841 tgcagacggc aacccaggtt cttgtagagc caaagataat cgtaggcctg taccagttgg
     3901 taggaaaact cggtgaacga catgccctcc ttcgtttcct cgccaaatcg cttctttacc
     3961 gagtcctttg ccatcatgta gttaacggtg atatgcttgc caacatcgcg gatgaaatcg
     4021 aggaacgaga aattcttcat ccagtcgtag ttgttcacca taacagctgc gttggggtta
     4081 ccgctgtcga agtcgagaaa ccgggaaagc tgattccgga taccctccag gtttttctgt
     4141 agggtggcgc tgtccagcag gtttcgctcc tgcgacttgc cgctggggtc gccaatcatt
     4201 ccggtagcgc ctccaaccag cacaatgggc ttatgcccgc atagctgaaa cctcttcagc
     4261 agcatgatct gggcgaagct gcccacatgt agagactccg cagtggggtc gaatcctata
     4321 tatcccgagg tcatctcctt attcagctga tcggccgttc ccggcatcac gtcgtgaacc
     4381 agtccgcgcc attggagctc atctatgaaa ttcatttcta ttgtaaaatt agtccggtgc
     4441 aggaatatca aaaaaacgtc caaatataag cgtttttgtc ccaaaagtaa ctacagaaag
     4501 tcgagtgtag gtgaaaagga aggtcgaacc tacttaccct tctggttccc gagggatttt
     4561 ttaaccgatt ccttaattgt gtcgaaatga aggtagggga atattgccgg agcaaggctt
     4621 gcaacaggat tgtacaccag cgattttgat ttttgctcct cagtgaagaa gttgaaacgt
     4681 atattagcct tttcaacaaa aaccagcagc acgctggtaa taatagcgta cttggcaaag
     4741 ccgaaaacaa acccaagcac tttattaatt ccccccagca tggccaaatt caaaaacctt
     4801 tcaactatct tttcaataaa gtggatggct attacaatta aaatgaaggt tacggcaaac
     4861 gaaatgatgg ggagatgagc cgagccaatg tcaaactttt ccgaaagcag ggtggccatg
     4921 tagtctgaaa aacgtactgc accccaaata cccagtagaa gagcaactac cgatgccagc
     4981 tgggacagga atccctgcct taatcccctt atggcgccca caaccaggag aattaccaca
     5041 acaatgtcaa atactgccat tagaagggtt gttaggatta gaaccttgtg gaggtgcagc
     5101 tgcccacaca atccaaaatc tttcccaaat tctcgttacg cagaaagtac aggttgttct
     5161 ttccctcgcg cttgcaggcg agaagcccct tatcctttaa aattccaagg tggtgtgagg
     5221 cggttgactg ctctatttgg agtttttcat gaatttgcgt aaccgtaagc ctcccattag
     5281 cctccagtaa gctgatgatg gctatgcgca tagggtgggc cattgccttt agcatgttgg
     5341 cggcatcttc gagctgctca accttcaact catttatctt taccatttaa aaaatactgt
     5401 taatagttcc atccgcacaa aatttagtgc tataaattta caattagcca cgctttggca
     5461 tttctatctt gttaaccttc ggatgaagca cccattgttt ggattcgccg aatccgcaag
     5521 ttacgctcgg ttgttcgtcc ttttttattc aggcttactc acaaaggtgt tttatattaa
     5581 atccatgcaa atatattaat attttactac aaacagaatt ttatgcccgg cctgtattta
     5641 ccagccaagc ctcctcacca gcttcgcaga ggtatttacc caattccaca ttctaccagt
     5701 tgtcgaatat caaccccgag gaggagcaag ttgaaaggtt agtacaggcg aaaggcacca
     5761 ataaaaaata agtgtattcg gaacttcatg taataacaag ggaaaatcaa caaaaatggc
     5821 atttgccaaa tgtcggctaa acttttattg agctttagcg ttaaatgaat agccaaaaaa
     5881 agtgttaatt taccgcttaa tattttcaac attccaggaa tgctcgaagc cattaaacag
     5941 ccagttgacg aggagctgaa aaaatttgaa ccctttttca ggaagaacat gggctcaaac
     6001 attcgtttcc tcgacctgat taccaactac atataccgca acaaggggaa gcagctacga
     6061 ccaatcattg tatttctatc ggctgggttg aacggcaaga tttgccagtc aacctatgtt
     6121 gctgccggta tgattgagct gctgcacact gccacgctta ttcatgacga cgttgttgat
     6181 gagtcgtttg agcggagggg tgctttttcc atcaatgccc tctggaggtc gaaggtagcg
     6241 gttctggttg gcgatttttt actttccaaa ggcttgctgg tggccgttca gaataaggag
     6301 tacgagttgt tggaggtgat gagcaacgct gttagggaga tgagcgaggg ggagttgctg
     6361 cagatagagc actcacggaa aatggatatt aacgaggagg cttactacga tataatccgt
     6421 aagaagacgg cctaccttat agcctcgtgc tgtgagaatg gggcaatttc ggctggtgcc
     6481 gattccgatt ccataaggcg aatgcacgaa tttggtatta acctgggaat ggccttccag
     6541 attagggacg atctcttcga ttaccagccc aaaggcctgg ttggtaaacc caacggcaac
     6601 gacatcaagg aaaagaagct aaccctaccc ctgatttatg cgcttaggga ggcacccgct
     6661 gctgaaggca gaaaagtact gcgtgaaatc aggagcagaa gtaaaagcag cgttaccatt
     6721 tcaacagtgg taaagtttac aaatgaatat ggaggaatta gctatgccac cggacagatg
     6781 ctaaacttca agcagcaagc ccaggaagtg ctcaacagct ttcccgattc ggattaccgc
     6841 caatcgctga tgcttctcac cgattacgtt actgaccgca acaagtaacc cctaaagtgt
     6901 tgggaggttt atccccttaa tgatcctgta ggtttcgagc gcatacacat cagtcatccc
     6961 cgacacaaag tcgagtaccg acataatctt agtgtagtca tcatcactgg aagtcatgta
     7021 ctggtttgga acaagccgta tgaccttttt ggatagcccg gtttgtggat ggagaacggc
     7081 atcgcagaaa atttcgagca gcgaccccag gatttggtag cctgctattt caatctcaac
     7141 caccttcctg tggttgtaaa ctctctccac cgccaggttt ttaatagcct ccatagccgt
     7201 atggcttgct cccgatattt tttcgagcaa cgatgaaaag tcttctccat ccatgattgg
     7261 cttcaggtta tccacaaata tccgagcaca ctcctccacc agcttgctga tgacaatagc
     7321 cctaaggtag gccacctgtt cgttgggatc ctgaacctcc tttagcgtag aggtaattct
     7381 atcccttgca cttttgggct catcggtaaa aaaagcacgg tagaggtcaa ccaccattgg
     7441 cgtggaaagg atgcccagct tatgggcatc ctcaatatcc ataatctggt agcagatatc
     7501 atcggcagcc tcgacaaggt aaaccaacgg atggcggcaa aattccatat tgcctacact
     7561 atgcattccc gttgcatgtg caatgctcag aaagggattg gcctccgact taaaaaaacc
     7621 gaacttcttt ttgcccgaag ctgatgggta gggatattta accactgaag ccaacacggc
     7681 attgctaagc ctgaatccac ctgtccgccg cccggttagc tgctgggtaa ggagcctaag
     7741 ggcgttggcg ttgccctcaa atcgcaccaa atcggaccat tcccagctgt ccaccatcgg
     7801 tttgaaaacc ccgctgttat cctcaaaaaa ctttcgtatg gcctcctcac cgctgtggcc
     7861 aaaaggcggg ttgcccatgt cgtgggccaa gcatgcagct gcgatgatgc ctggtatttc
     7921 ggcaaccagt ggtttttcat gcggactaag ggtttcggat atctgcaggt aaacattctg
     7981 tcccagcgat cggcccacgc tcgacacctc aaggctatgg gtaaggcggt tgtgaacaaa
     8041 tatgcttccc ggcaaaggga atacctgtgt tttgttctgc aaccgacgga agggagagga
     8101 aaaaataagc cggtcgaagt cgtgctggta gttggttcgg gcggtatcgt aagtgttttt
     8161 agttgccccg gttgcgccaa accggctggc atcgagtagt tttgtccatt ccatttgttg
     8221 gcataaattg actgatgttt ggcactacct tttagacaga aaaaggaata ccttattcgg
//
LOCUS       DAOOQX010000006         8243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_10718_length_8243_cov_3.220445, whole genome shotgun sequence.
ACCESSION   DAOOQX010000006 DAOOQX010000000
VERSION     DAOOQX010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8243
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10718_length_8243_cov_3.220445"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1537
                     /locus_tag="PLX53_00210"
     CDS             185..1537
                     /locus_tag="PLX53_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY71533.1"
                     /translation="MKQMFLILQREFMTRVRKKSFIFTTLLSPLFFAAMVILPTYFAT
                     MEDTEVRNIAVIDNTQHFGSAIPQTEYIKITTITDTPAEKVKNSLEESGYYALLTIDS
                     LPGSIAPALTIYSPKQPSIEVISHIENSMEKEIETRKLMTYNIQDLDKILKDVKTKVN
                     VRSIKISKDGEEKESNTLITMGIAYILSFLMYMMVMLMGNQVMQGVIEEKNNRVVELL
                     VSSVKPVQLMMGKIFGIAAVGLLQIAIWIVLTAGLAGVGLNLISEKSGKANMEQVQIA
                     NQSIMANQGAQSADVTAAISETNTDNFLQALKNQNYGLIIGGFIFFFLMGYLLYAAMY
                     AAVGSAVENVSDTQQLVMPITIPLILAIFVMIVGIKSPDGSLAFWFSMIPFTSPIVML
                     TRLPFGVPVWQLILSAALLIGTFAVTVWLAAKIYRVGILMYGKKYSWKEMIKWIRYRS
                     "
     gene            1636..2277
                     /locus_tag="PLX53_00215"
     CDS             1636..2277
                     /locus_tag="PLX53_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOY71534.1"
                     /translation="MDNDLQQVYQSVKFACRGDSTGHDWWHILRVVKLALFISERENA
                     NPFTVELAALLHDVDDWKLNRNTEGCPVALRILHDSGYSDDEARRVALIIGQVSFKGA
                     GVDTTPSTIEAKVVQDADRLDAIGAIGVARAFAYGGARQRPMYIPDLKPQQHHSFDEY
                     QHAHGSTINHFYEKLLLLKDRLNTQTARQIAVERHQFMELFLTQFFKEWSVLD"
     gene            2289..3218
                     /locus_tag="PLX53_00220"
     CDS             2289..3218
                     /locus_tag="PLX53_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71535.1"
                     /translation="MNVAIIDMGTNTFNLLVARFNQEERPEIIYEEKLPVRLGEGGLN
                     RKIITPEAWQRGLDAINTHMAKAKSLGAGKFFAFATSATRDASNGTNFVEDIRTSFGL
                     NVEIIDGNREATLVYKGVREALDLGNTCSLILDIGGGSNEIILGNTSEPFWLHSFNLG
                     VSRLMQQFQLSDPITQKQLRALEEFINVEVQTLFDAARRYVPVRLVGSSGSFDSIRSI
                     MEHSGSIAPGSEPWAEIPLERYFELHNFFITSTHDQRMALKGLDPMRVDLIVPASVFI
                     NLIIQKIGINKLFQSSFALKEGFMAELQSSHAF"
     gene            3265..4428
                     /locus_tag="PLX53_00225"
     CDS             3265..4428
                     /locus_tag="PLX53_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOY71536.1"
                     /translation="MAKILVIDDEKAIRSTLKEILEFESHSVDLAEDGPTGIEMFGSG
                     CYDIVLCDIKMPQMDGIEVLEKLQEQAADVPVVMISGHGNIDTAVESIKKGAYDFIEK
                     PLDLNRLLITIRNATDKTTLIQEAKILKRKVNKAYDIVGESPAIVKVKEMIDRVAPTE
                     ARVLITGPNGTGKELVARWLHEKSNRSDMPFVEVNCAAIPSELIESELFGHEKGAFTS
                     AIKQRKGKFEQADGGTLFLDEIGDMSLSAQSKVLRALQENKISRVGSDKDIPVNVRVI
                     AATNKNLTHEIQNGNFREDLYHRLSVIIIHVPPLSERVEDIPLLAEHFNQLICSEYGI
                     PPKVFRPDAVDELKRLPWTGNVRELRNVVERLIILCDKDITGGDVKAFASPAF"
     gene            complement(4441..5028)
                     /locus_tag="PLX53_00230"
     CDS             complement(4441..5028)
                     /locus_tag="PLX53_00230"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HOY71537.1"
                     /translation="MVKKILMLLLATLMCPALYAIPDTSRVALLVIDIQDFYFPGGKS
                     ELVNPLPAAKNAALLVEYFRSHQLPVIYVKHQSAEQEEINEMVKPLADEPIFTKTEVN
                     CFVGTQLYDYLVGQQISKLVICGMQTHLCVEAAVRAASDYGFKVTLIHDACATRDVKW
                     GNTTIPANMVHLSTLSTLQNYARILSTEECLKANP"
     gene            complement(5210..5671)
                     /locus_tag="PLX53_00235"
     CDS             complement(5210..5671)
                     /locus_tag="PLX53_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HOY71538.1"
                     /translation="MRTLGNILWHFPFLGFLTALGTFLIGGLLVITVIGSPLGLGLIQ
                     LSKFLLAPFSNAMVDKTDLQADQNKAWKTYGIIVRILYFPIGLFLVVVNIFQIAGLFI
                     SIVGIPVALVLAKSIGTYFNPVNKVCVSKAVADELSSRKAKAEVDKAFATK"
     gene            complement(5886..7013)
                     /locus_tag="PLX53_00240"
     CDS             complement(5886..7013)
                     /locus_tag="PLX53_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71539.1"
                     /translation="MRYVLLFTLMLVVASVAAQEPVTLKFTRPSKVQASASKIKLKVQ
                     GNEYVLKNGASISINVTPEYGKSLLINAGDQTAYYLKPKPGETYEFEVGFKFSGPYIL
                     LLSGEEAKPGEAVQVTDTTAKPEGWETNLKVDRDNMGAGITLQKTDQSEAIRQEWLAR
                     GGKIKYQSVVFNGTYFGMDMDMEGVDANRIDGYGGGISFYQNRINLKIPAYKTGASSW
                     NSFNIGFGYDMLVYAYKFDMKMDPITTKMDVVTITMPFSANIGWTLGLGKFIDEGNWK
                     GVALTLRYRPSIMFNFTNSTVKMTSTDPHISGSTSTSTDSNVQFNAGGVGFDVDFSNF
                     SSTMEKLAPKPKLKLSFFVMPPVKDMPLFISFGLGITTYSR"
     gene            complement(7243..7497)
                     /locus_tag="PLX53_00245"
     CDS             complement(7243..7497)
                     /locus_tag="PLX53_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOY71540.1"
                     /translation="MTSKEEIVTKVAAAIDVIRPFLQNDGGDISLVEVTDDNTVRVKL
                     HGACGSCPYSLMTLKNGVEQAIMRDVPEIRGVEAVNLNIP"
     gene            complement(7527..>8243)
                     /locus_tag="PLX53_00250"
     CDS             complement(7527..>8243)
                     /locus_tag="PLX53_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOY71541.1"
                     /translation="ARVGLIDADIYGPSIPKMFNVENVQPEVMKEDEMELIVPVERYG
                     VKLLSIGFFVDPNNALIWRGPMATSALKQLIHQGAWGELDYLFIDMPPGTSDIHLTLV
                     QEVAVTGAVIVSTPQDVALADAVKGISMFGSQGINVPVLGLVENMAWFTPEELPENRY
                     YIFGKDGCKKLAERMKLPLLGQIPLVQSIREGGDFGEPVALHSSVVAKAFDELATNLI
                     RQVEIRNRELPPTTIVNVKH"
BASE COUNT         2188 a         1829 c         1959 g         2267 t
ORIGIN      
        1 ggtaaggctg caatcggctt cggatagcaa caagctgctt caaagcctga tttcaagtgt
       61 tgaggtgctt tcgtttaatc ccgtagtacc cagcatgcac gatatcttta ttcgggttgt
      121 gcaggagtac aacaaggctc atggtttatc gcttaactag tattttataa aaaattccgg
      181 aattatgaaa caaatgttcc ttatactcca gcgggagttt atgacccggg tgcggaaaaa
      241 atcattcatt tttaccaccc tgctttcgcc gctatttttt gccgccatgg taattcttcc
      301 tacctatttt gccaccatgg aggacactga agtgcgcaat attgccgtta ttgacaatac
      361 acagcacttt ggaagtgcta ttccccaaac cgaatacatt aaaattacta ccataaccga
      421 tacgccggcc gaaaaggtaa aaaattcact agaggagtca ggctactacg ccctgcttac
      481 aattgattca cttccgggta gcattgcccc agcccttacc atctactcgc ccaaacaacc
      541 ttccattgag gtgatttcgc atatcgagaa ctctatggag aaggaaattg aaacccgtaa
      601 gttgatgacc tacaacattc aggaccttga taaaatactc aaggacgtaa aaactaaggt
      661 taacgtccgc tccatcaaaa tttcaaagga tggcgaggaa aaggagagca ataccctgat
      721 aaccatggga atagcttata ttttaagttt cctgatgtac atgatggtga tgctgatggg
      781 caaccaggtt atgcaggggg taattgagga gaagaataac cgcgttgttg agctactcgt
      841 ttcgtctgta aagcccgtac agcttatgat gggcaaaata tttggaatag cagcggtggg
      901 gttgctgcag attgcaattt ggattgtact cactgccggt ttggccggag ttggacttaa
      961 cctcatatcg gaaaaatcag gcaaggccaa tatggagcag gttcaaattg ccaaccagtc
     1021 catcatggcc aaccaaggag ctcagtcagc cgatgtaaca gcagcaatct ctgaaacaaa
     1081 taccgataac tttctacaag ccctgaaaaa tcagaactat ggattgataa tcggcggttt
     1141 tatattcttt ttcctgatgg ggtacctgct ctatgccgcc atgtacgctg ctgttggttc
     1201 ggctgtagaa aatgtttccg acacccagca gctggttatg cccataacca ttccccttat
     1261 actggcgata tttgttatga ttgttggcat taaatcgccc gacggttccc ttgccttctg
     1321 gttctcgatg atacctttca cctcccccat agtgatgcta acccggcttc catttggcgt
     1381 tcctgtatgg cagcttatcc tttcggcagc gctactaatc ggaacttttg ctgttactgt
     1441 atggttagct gcaaaaattt acagggtggg aattctgatg tacggcaaga agtactcgtg
     1501 gaaggagatg ataaagtgga ttaggtatag aagctagcag caggtaataa gcttgttgag
     1561 ctgagggcga ataatccatc ggattttcgc tcttcgcatt ttgaagcgct ttttttacaa
     1621 acttgtatta ccccaatgga taacgattta caacaggttt accaatctgt taaatttgcc
     1681 tgccgcggcg attcaacagg tcacgactgg tggcacattc tcagggtggt taagctggcg
     1741 ttgttcatat cagaaaggga aaatgccaat cccttcacgg ttgagctggc cgctttgcta
     1801 cacgatgttg acgattggaa gctgaacaga aacaccgagg gttgccctgt tgcgctcaga
     1861 attctgcacg attccggata tagcgatgat gaagcccggc gtgttgcact tatcattggg
     1921 caggtatcat ttaagggtgc cggtgttgat actactcctt ccactattga ggccaaggtt
     1981 gttcaggatg ccgaccggct cgatgccatc ggcgccattg gagttgcaag ggcatttgcc
     2041 tacggcggag ccaggcaacg gcccatgtac attcctgatt tgaagcccca gcaacaccac
     2101 tcgtttgatg agtaccaaca tgcccacgga agcaccatca atcactttta cgaaaagctt
     2161 cttcttttga aagataggct caacacccaa acggcaaggc aaattgctgt tgaaaggcat
     2221 cagtttatgg agctgtttct tacgcagttt tttaaggagt ggagtgtttt agattagatg
     2281 tttaggttat gaatgtggca attattgaca tgggaaccaa caccttcaac ctgttggtag
     2341 ccaggtttaa ccaggaagaa cgccccgaga ttatttacga ggaaaagctc ccggttaggc
     2401 tgggcgaggg cggtttaaac aggaagatta ttactccgga ggcatggcag cgtggcctgg
     2461 atgcaattaa tacccacatg gctaaagcga aaagcttggg tgctggtaag ttctttgcat
     2521 ttgccacttc ggctacccgg gatgcctcaa acgggacaaa ctttgttgag gatattcgca
     2581 ccagttttgg gctcaatgtt gaaataattg acggtaatcg cgaggcaacg ctggtgtaca
     2641 agggggtcag ggaggcatta gatttgggta acacctgtag cttgattctg gatattggtg
     2701 gtggaagtaa tgagataata cttgggaata ccagcgagcc attttggctt cacagcttta
     2761 acctgggtgt ttcaaggttg atgcagcagt ttcaactttc cgatcctata acacagaagc
     2821 agctgagggc tctggaagag tttattaatg tggaagttca aactcttttt gatgctgcgc
     2881 gtaggtatgt tcctgtccga ttggtgggaa gctccggctc tttcgattca attcgtagca
     2941 tcatggagca ctcgggaagc attgctcctg gtagcgaacc ctgggccgaa ataccccttg
     3001 agcggtactt cgagctgcac aattttttta tcacctccac ccacgatcag cgcatggcgc
     3061 taaagggact ggaccccatg cgggttgacc tgattgttcc ggcctcggta tttattaacc
     3121 tcatcattca aaaaatagga attaacaagt tgttccagtc atcattcgcc cttaaggagg
     3181 gatttatggc cgagctgcaa agcagccatg ctttttaatc acgttcatta agcattattt
     3241 ggtatattta ccaaaaattg agctatggcc aaaatccttg tcattgacga cgagaaggct
     3301 atccgaagca ccctgaagga gatacttgaa ttcgaaagcc attcggttga tttggctgaa
     3361 gatggcccaa ccggaattga gatgtttggc tccggatgct acgatattgt tctctgcgac
     3421 attaaaatgc cccagatgga tggcattgag gtccttgaaa agcttcagga acaagcagcc
     3481 gatgtaccgg tggttatgat atccggacat gggaacattg acactgctgt ggagtctata
     3541 aagaaggggg catacgactt tattgagaaa ccgttggacc tgaaccgttt gctaatcacc
     3601 atacgcaacg caaccgataa aactaccctt attcaggagg ccaaaatcct taagcgcaag
     3661 gtgaataagg cctacgacat agttggcgag tcgccggcta tcgttaaggt caaggagatg
     3721 atagacaggg ttgccccaac cgaggcaagg gtgctcatca ccggaccaaa cggaacaggg
     3781 aaggagctgg tagcccgatg gcttcacgaa aagagcaacc gctccgacat gccttttgtg
     3841 gaggtgaact gtgctgccat tccatcggag cttatcgaga gcgaactttt tggacacgag
     3901 aagggagcgt ttacatcggc aattaagcag cgcaagggta aatttgaaca ggccgatggc
     3961 ggaacgcttt tcctcgatga aataggcgac atgagtctaa gcgctcaatc aaaagtgctc
     4021 agagctcttc aggagaacaa gattagccgt gtggggagcg ataaggatat tcccgttaac
     4081 gtgagggtta ttgcggcaac caataaaaac ctcacccacg agattcaaaa tggtaacttt
     4141 cgcgaagacc tttaccaccg tttaagcgtc atcatcattc atgttccccc gctaagcgaa
     4201 agggttgagg atattccatt gcttgcagaa catttcaacc agctaatttg ctccgagtat
     4261 ggcataccgc ctaaggtgtt tagacccgat gctgttgacg agttgaaacg gctaccctgg
     4321 accgggaacg taagggaact tcggaatgtt gtcgaacggc ttatcatact atgcgacaag
     4381 gacataaccg gaggcgatgt taaagctttt gccagtcctg cgttttaagt ggttaaatct
     4441 ttaagggttg gccttgaggc attcctcggt actaagtatt ctggcgtagt tttgaagggt
     4501 tgaaagggtt gacaggtgaa ccatgttggc cggaatggta gtattgcccc atttaacatc
     4561 cctggtggca cacgcatcgt gaataagggt taccttgaac ccatagtccg aagcagccct
     4621 aactgctgcc tcaacacaga ggtgggtttg cataccacag ataaccagct ttgaaatttg
     4681 ctggcccaca aggtagtcgt aaagttgtgt ccccacaaag cagttcacct ccgttttggt
     4741 gaaaatcggc tcatcggcca gaggctttac catctcgtta atttcttcct gttctgccga
     4801 ctggtgtttt acgtatataa ccggcagctg gtgcgatcgg aagtattcaa ccagcagcgc
     4861 tgcattcttt gccgcaggca acgggtttac cagctccgat ttgccacccg gaaagtagaa
     4921 atcctgaata tctataacca gcaacgcaac ccttgacgtg tcgggtatgg cgtatagtgc
     4981 cggacacatt agggtagcaa gtagcagcat gaggattttt ttaaccatat ttttgggctt
     5041 tattttgtaa atataaatgt atttatagta aacttttatc aaaacggcct ttattttcac
     5101 tatgctttta caaaaaagaa acctccctgg ttgctgtaac aagccaagga ggatagggca
     5161 tttaaaatgc ctttttaaat ttgttggatg gaatgttatg ctacattctt tactttgttg
     5221 caaaagcctt atcaacctct gcctttgcct ttcttgagct aagctcatcg gcaactgctt
     5281 ttgaaacgca aaccttgttt accgggttga agtaagtacc aattgatttt gccaaaacca
     5341 gagccactgg tatcccaacg attgaaataa ataggccggc aatttgaaaa atgtttacaa
     5401 caaccagaaa tagcccaatc gggaagtaaa gaatccggac aataatcccg taagtcttcc
     5461 aggccttatt ttgatcggcc tgaaggtcgg ttttatcaac catggcgttg gagaatgggg
     5521 caagcaagaa cttggaaagc tgaattagcc ccagaccaag tggtgagcca attacagtta
     5581 taaccagcaa accgcctatt aaaaaggttc caagtgcggt taaaaacccc aaaaaaggaa
     5641 aatgccataa aatgtttcct agtgttctca taaaattaga gttaagttga attttttcct
     5701 actcgtttgg cttttcggta acgcccctct tggttatagt ggttttagga ctccactgtt
     5761 tatttctatt gtaattatag ttctgatgtc gggtttggcc caagactttt agattatcgg
     5821 ggtatgacct ttagcttgta atcagcatac tgataacttt tgggtgtgtt ttcatttata
     5881 aattactagc gggagtatgt agttataccc agcccaaagc tgataaacag gggcatgtcc
     5941 ttaacggggg gcatgacgaa gaacgaaagt ttaagcttgg gtttcggggc gagcttctcc
     6001 atggtggagc taaagtttga gaaatcaacg tcgaagccca caccaccagc gttaaactgc
     6061 acattgctgt cggtgctggt ggaagtagag ccggagatgt ggggatcggt tgaggtcatc
     6121 ttaacggttg agttggtaaa gttaaacata atggaaggac ggtaccttag ggtaagggca
     6181 actcccttcc agttcccctc atcaataaac tttcccaaac ccagtgtcca tccaatattg
     6241 gccgagaatg gcatggtaat agtaaccaca tccattttag ttgttatggg gtccatcttc
     6301 atgtcaaatt tgtatgcgta caccagcatg tcgtatccaa aaccgatgtt aaaggagttc
     6361 catgatgaag ctccggtttt gtaggcagga attttaaggt taatcctgtt ctggtagaac
     6421 gaaatgccgc ctccgtaccc gtctatcctg tttgcgtcaa cgccctccat gtccatgtcc
     6481 ataccaaaat aggttccgtt gaaaacaacc gattggtatt taattttacc cccgcgagcc
     6541 agccactcct gccttattgc ctcggactgg tcggtttttt gaagggtaat tccagctccc
     6601 atgttatccc ggtcaacctt taggttggtt tcccacccct cgggtttggc agtggtgtcg
     6661 gttacctgga cagcctcgcc tggtttggcc tcttccccgc tcagcagcag tatgtaaggg
     6721 cctgaaaatt taaaaccaac ctcaaactcg taggtttccc caggtttagg tttcaagtag
     6781 taggcagttt ggtctccggc attaatcagc aacgactttc cgtactccgg ggtaacgttg
     6841 atggatatgc ttgctccatt cttaagaacg tattcgttac cttggacttt cagcttgatt
     6901 tttgatgctg aagcctgcac ctttgatgga cgcgtaaatt taagcgttac tggttcctgc
     6961 gcagcaactg aggcaacaac cagcatcagg gtaaaaagta gtacgtatct cataggttga
     7021 aagtattaat gataatttgg tgtaaatgtc gatggggaac tttgcttttg caagggtttg
     7081 cgtttgattt ttgacagacc cggggaaaaa gttgatggga ttataatttt atgattgcca
     7141 accacatatt ttatactaca aaaaaactcc ctttaaaaag ggagttttcc gttacctgaa
     7201 gttctagaat tctaaatggt taggatactt gaaaaaaagt tgttagggta tgtttaggtt
     7261 aactgcttca acccctctta tttcgggaac atcgcgcatg atggcttgtt ctactccatt
     7321 cttcaaagtc atcaggctgt aggggcagct accgcatgcg ccatgcaact tcaccctaac
     7381 cgtattgtca tcggttactt ctaccagtga gatgtcaccg ccatcattct gaagaaacgg
     7441 gcgaataaca tcaatggctg cagctacttt tgttacaatt tcctccttgc tagtcatggt
     7501 ttaaaaattt tgaaatggat aatttattag tgtttaacgt ttacaatggt agtaggagga
     7561 agttccctgt tgcgaatttc gacctgtcta ataagatttg tggcaagttc atcaaatgct
     7621 ttggccacca ccgatgagtg cagggcaacg ggttcaccga aatcgccacc ctcacgaatg
     7681 ctttgcacca gggggatttg ccctagcaac ggtagtttca tgcgctcagc cagctttttg
     7741 caaccatcct ttccaaaaat gtagtaccta ttctcgggta gttcttcggg agtgaaccat
     7801 gccatgttct ccaccagccc aagcactggg acatttatcc cctgacttcc aaacatgctt
     7861 atccccttaa cagcgtcagc caaagcaacg tcctgggggg tgctaacaat aacggcaccg
     7921 gtaaccgcaa cttcctgaac cagggtcagg tgaatgtcgc tggtgccggg tggcatgtca
     7981 atgaacaggt agtctagctc accccaggct ccctggtgga ttagctgctt tagtgcgctg
     8041 gtggccatgg gtccccgcca aataagagca ttgttggggt caacgaaaaa accaattgag
     8101 agaagcttca cgccgtaacg ctcaacggga acaatcagtt ccatctcatc ctccttcata
     8161 acttcgggtt gaacgttttc tacgttgaac attttaggaa tggagggtcc gtatatatcg
     8221 gcatctataa gtccaacacg ggc
//
LOCUS       DAOOQX010000007         8198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_10791_length_8198_cov_3.396905, whole genome shotgun sequence.
ACCESSION   DAOOQX010000007 DAOOQX010000000
VERSION     DAOOQX010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10791_length_8198_cov_3.396905"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1293
                     /locus_tag="PLX53_00255"
     CDS             226..1293
                     /locus_tag="PLX53_00255"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOY71542.1"
                     /translation="MKPLNRIFIFLLLLLSVAKPSSAGRVVPNSINRSSIREIEASIR
                     NGDYGKITSIVVLNRQGAILYEKYFGFSTRNSLHTISSVTKSITSLMVGICLDKGFIS
                     SIDSPIYSYFPEYKEAFEKDSTKKLITLRHLLQQTAGLNFKEWQFPYNYASNSLMAAI
                     EENRSWVERFFYLPVDTLPGVKFSYNSLASQVIAEVLSRASGIAYDEMVREFLFKPLS
                     IGNFLWESYAGNSLPAWGGLSLTTFDMAKIGLLVINGGIYGGRRLVSSEWVESSTVGR
                     VRVNDNIYYGLHWWVDILPSGKPLPFAAGYGDQYVFTAPDRGLVVALNAQNFSDFRFS
                     KSAIDLANILLSSVEVENFQH"
     gene            1305..2315
                     /locus_tag="PLX53_00260"
     CDS             1305..2315
                     /locus_tag="PLX53_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOY71543.1"
                     /translation="MSDLKTSYMGIELKNPLIAGASSLSLNIESLKAIEAAGAGAVVF
                     KSLFEEQIQLEALELENELEEYNERHAEMVKLFPTLKHAGPADHLAKLRKAKDTLSIP
                     VIASLNAMYPDTWVEYAKEIEQTGVDGIELNFYTTPKNFEVEGKTIIQSQVDTLKSVL
                     EKVTIPVSVKLSPFYTNPLDVIRRFDETGIQGFVLFNRFFEPDIDIEKEELVQTITLS
                     NEDDLRLPLRFTGLLYGNIVADICSSTGIMTGADAVKMLLAGATTFQVTSTIYANGIE
                     QITKILGEIEAWMHRKGYKSLHDFRGKLAKKNLHDPFAYLRAQYVDILLRSNDIIKDS
                     AI"
     gene            complement(2512..2784)
                     /locus_tag="PLX53_00265"
     CDS             complement(2512..2784)
                     /locus_tag="PLX53_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOY71544.1"
                     /translation="MTAKTKAISVKGRVQGVGFRYFVLRNAKRLGIRGFVKNMPDRSV
                     YIEAQGQEENLNELIGLCWKGPERSRVDSVETNEVVRKEWNDFYVE"
     gene            2898..3386
                     /locus_tag="PLX53_00270"
     CDS             2898..3386
                     /locus_tag="PLX53_00270"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOY71545.1"
                     /translation="MVVDYVLIVLAAALVVVGLVGCFLPVIPGPPLSWVGLLLAYFTN
                     FVNFDWSLLLWTGIATLVVVILDYVMPVWVTRKMGGTKRGVWGSAIGLVVGLFVFPPL
                     GIIVGPFVGAFIGELTARDKSNNPLLSATGSFIGFLVGTGLKFAVSGYIAYRFTVEVF
                     FR"
     gene            complement(3530..3805)
                     /locus_tag="PLX53_00275"
     CDS             complement(3530..3805)
                     /locus_tag="PLX53_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOY71546.1"
                     /translation="MMIYVGNIAYSMTPDDLKELFAPFGNVLSVKIIADKTSGRSKGY
                     GFVEMEKDEDGQKAISALNDTQVKGRNIKVNNAFRKDDSKTEGKQKD"
     gene            complement(3915..4916)
                     /gene="argF"
                     /locus_tag="PLX53_00280"
     CDS             complement(3915..4916)
                     /gene="argF"
                     /locus_tag="PLX53_00280"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOY71547.1"
                     /translation="MSFNLRNRSFLKLLDFTPKEIQFLLDLSADLKKAKYAGTEQQQL
                     KGKNIVILFEKDSTRTRCAFEVAAYDQGAHVTYLGPSGSQMGKKESMKDTARVLGRMY
                     DGIEYRGYSQEIVETLAKYAGVPVWNGLTTEYHPTQILADFLTMREHSSKPLSKVTFC
                     YMGDARNNMGNSLMVGAAKMGMDFRACAPKACWPKEELVKTCMEIAKTTGARITLTEK
                     VEEGVKGCDFLYTDVWVSMGEADSVWDERIKLLKPYQVNRKAMELTGNPNCKFMHCLP
                     SFHNRETAVGEDIFKKFGLESMEVTDEVFESDASIVFDEAENRVHTIKAVMVATLGS"
     gene            complement(5078..6037)
                     /gene="arcC"
                     /locus_tag="PLX53_00285"
     CDS             complement(5078..6037)
                     /gene="arcC"
                     /locus_tag="PLX53_00285"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOY71548.1"
                     /translation="MSKLAVVALGGNALLRGNQVGTIEEQEQNTTETLENLVYLIKEG
                     YNLVISHGNGPQVGNILMRNDAGEQLYGIPQMPLDVCVADSQGGIGYMIERMLRNVLK
                     NHGIDKEVCCLVTPVIVDKNDPAFSNPTKRVGRIYDKAQADALASQKGWIFKEEVKDT
                     GSGWRRVVPSPKPKGILNIKVIDQLVDQGIIVIASGGGGVPVYIDEEKNVRPAEAVID
                     KDSASALMDAEIKADEFYILTDVPFVFLNYKKPNEKKLEFLNHADATRYLQEGMFGEG
                     NMAPKIRACLNFIENGGKMSVITEATKLEDMSYGTKITMAYED"
     gene            6359..7051
                     /locus_tag="PLX53_00290"
     CDS             6359..7051
                     /locus_tag="PLX53_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71549.1"
                     /translation="MRKPIAGLVILSAFILCSCGGRVKNNSISEGVITYDITYDSAIQ
                     SRYDAWLLPSELIVRFNSNHSINSIEALGGAISISIIKNPERNEFSTLLKIFNKKLYH
                     SEPIEQERYPALYSRMPAVKLISEGETCEFLGYSCTKVKGYFADNPDFRFEIIYTSQI
                     QIENPNAHTPFEEVKGTLLQFNIRLNNLTMHATARSVKSEHVPLELFAPPSDYVVTDS
                     QTIANLIYTLQQ"
     gene            7281..>8198
                     /locus_tag="PLX53_00295"
     CDS             7281..>8198
                     /locus_tag="PLX53_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOY71550.1"
                     /translation="MASDSNRPNVKEKNNATKEGQDNGKNKLLRDERVRFTIGLFLLL
                     LGVYFLISLISYLFTWKVDQSLEWQSLFSKAEYEVKNWTGRFGAVISAQFMNHWFGLS
                     SLALPSIIIVFGLKLLKLYRKNIWRFIFNIFIGTLLCSLVLGFLFETAKGFLGSGLGG
                     AQGLFVSKWLSALLGKVGTSFLLLLLVIAYTIFIKPSTLHWFRRTIRGLLIRMRRPVS
                     RKNDKRQNKKEKTAEELAGQVDSEVLSNNTDAAVEVTLEKFIPEPNSELVDAEPFELI
                     EEETAGTNSSNAENTPEKSDVEFTIEKINE"
BASE COUNT         2103 a         1785 c         1835 g         2475 t
ORIGIN      
        1 gtgtttcacc gagttaagca agctgcctaa ggatttagga tagcagcttg agtttaatga
       61 gcaactattt ccccggaaaa gtagccgggg aatttttttt atgtcctatt gcgatgatcg
      121 cgctgcccgg tgtgaacaaa atttgtcagg agcggtttat tgcaaaaggg tttgggttgt
      181 ctggtttata ttttttgccg taactttagg ttaaagtttt ttcggatgaa acccctaaat
      241 cgaatattca tttttttgct tctgcttttg tctgtggcaa aaccatctag tgcgggcagg
      301 gtggtaccta actcgattaa caggtcatct atcagggaaa ttgaagcctc aattcgcaat
      361 ggagactatg gtaagattac cagcatcgtg gttttaaaca ggcaaggtgc tattctttac
      421 gaaaagtatt ttggattctc cacccggaat tcacttcata ccatcagctc ggttaccaag
      481 agtattacct cgctaatggt gggaatatgc ctcgacaagg gctttataag ttctattgat
      541 tcacctattt acagctattt ccccgagtat aaggaggctt ttgagaaaga tagcaccaaa
      601 aagctcatca ctctgcgcca tttgcttcag cagactgcag ggttgaactt caaagagtgg
      661 cagttcccct ataactatgc ctctaacagc cttatggctg ccattgagga aaaccggagc
      721 tgggtggagc gctttttcta cctgccggtt gacacgctac cgggtgtaaa attttcttac
      781 aacagccttg cctctcaggt aatagctgag gtgctctcac gtgcctctgg aatagcttac
      841 gatgagatgg ttcgggaatt tttattcaag cccctgtcta ttggaaattt cttgtgggag
      901 agctatgcag ggaatagcct acctgcttgg ggaggattgt ccttaactac cttcgatatg
      961 gccaaaatcg gtttgctggt aattaacggg ggtatatatg gcggtaggag gttggtttcc
     1021 agcgaatggg tggagagttc aacggtgggc agggtgaggg ttaacgataa catatactat
     1081 gggctgcact ggtgggtaga tattttacca tcgggaaaac cattgccatt tgccgccggg
     1141 tatggcgatc agtatgtttt tactgcgccc gataggggtt tggttgttgc gcttaatgcg
     1201 cagaactttt ctgactttcg cttctccaag tcggcaattg acctggcaaa tattttacta
     1261 tcatcggttg aagtagaaaa tttccaacat taaaaccgta tgccatgagt gacctgaaaa
     1321 ctagctacat gggaatagag cttaaaaatc cgctaattgc gggtgcatcg tctctgtcgt
     1381 taaacattga atcgttaaag gcaattgagg ccgcaggtgc aggagcagtt gtcttcaaat
     1441 cgcttttcga ggagcaaatt caactcgaag cgctggagct tgaaaatgag ctggaagagt
     1501 ataacgagcg gcatgccgaa atggttaaac tctttcccac gcttaaacac gcaggccctg
     1561 ccgatcatct ggctaaactt aggaaggcca aggacacgtt gagcattcca gtcattgcaa
     1621 gtcttaatgc catgtatccc gacacatggg ttgaatatgc caaggagatt gagcaaacag
     1681 gggttgatgg tatagagctg aacttctaca ctactcccaa aaattttgag gtagagggga
     1741 aaaccattat tcaatcgcaa gttgacacgc tgaaaagtgt cctcgaaaag gtgaccatcc
     1801 cggttagcgt taaacttagc ccgttttaca ccaacccact tgatgttatt cgccgctttg
     1861 atgagacagg tattcagggt ttcgtcctgt tcaatcgttt ttttgagccc gacattgata
     1921 ttgaaaagga ggagttggta cagaccataa ccctgagcaa tgaggatgac ctacgtttgc
     1981 cattgcgatt taccggtttg ctttatggca acatagtagc agacatttgc tcctcaacgg
     2041 gaataatgac aggtgctgat gctgtaaaga tgttgcttgc cggagcaact acgtttcagg
     2101 ttaccagcac tatttatgcg aatggtattg agcaaattac caaaattctg ggtgaaattg
     2161 aggcatggat gcatcgtaag ggttataaat cccttcatga tttcaggggt aagctagcaa
     2221 agaaaaatct gcacgatccc tttgcatacc tgagagccca gtacgtggat atccttctac
     2281 gttctaacga tatcataaaa gactctgcaa tctagcctaa aattgatttg gagattaatc
     2341 gaatttgctc tacagaaaaa tatcacttct agcaggtatt aatggagggg tgattgccca
     2401 agatataatt atactgtagc ctgttacccg ataactcgtt atgaaacagt tgtaattact
     2461 acattcttac ccttgcaatt tgctagagag gccaagttgt ttgtaaattc tctactccac
     2521 gtaaaagtcg ttccactctt tccttaccac ctcgttggtt tcaacgctgt ccactctgga
     2581 ccgttcaggc cctttccagc aaagaccaat cagctcatta agattctcct cctgtccctg
     2641 agcctcgatg tacacgcttc tatcgggcat attctttacg aaacccctga tgcctagcct
     2701 tttggcgttg cgtagcacga aatacctgaa ccccacgcct tgaaccctac cttttacact
     2761 tatggctttt gttttagctg tcatggctgg ttttgattat acaaatttag ggctatcttt
     2821 gtttgtattc aatgctgaaa ggatttcatg gagaaatttt tgaagcactt ttgaagtaga
     2881 acaaactaaa tgagtgtatg gtagtagatt atgtcctgat tgttttagcg gctgcccttg
     2941 tggttgttgg attggttggt tgttttctac ccgtcattcc tggccctccg ttgagttggg
     3001 taggattact gctggcctac tttaccaact ttgtgaattt tgattggagc ttgcttttat
     3061 ggacggggat agccacgctt gttgtagtta ttctcgatta tgtaatgccg gtgtgggtaa
     3121 ccaggaaaat gggtggaacc aagcggggag tatggggctc ggctattgga ttggtggttg
     3181 ggctttttgt ttttccaccc ctaggaatca ttgttggacc atttgtaggc gcattcatcg
     3241 gtgagctcac cgcaagggat aaaagcaata acccgctgct ctcggcaaca ggttcattta
     3301 taggatttct tgtaggtacc gggctaaaat ttgctgtttc gggttatatt gcctaccgct
     3361 ttacggtgga ggtttttttc aggtagccca aaagacatta ttgattttcc ttttaaactc
     3421 caaataaaca taatggtaaa accacaccaa cagcacatcg aatgttaact gcatggaaaa
     3481 aatcggaatt caaacttacc aggagttggc tttaccttta agcccgtggt tagtccttct
     3541 gctttccttc tgtttttgaa tcatccttcc taaaggcgtt attcaccttt atgtttctac
     3601 ccttaacctg ggtatcgttc agagcgctga tggctttttg gccatcctca tccttttcca
     3661 tttcaacaaa accgtacccc tttgatcttc cggaggtttt gtcggcaata attttaaccg
     3721 agagcacgtt tccaaaaggg gcaaatagct ctttcaagtc atctggcgtc attgagtagg
     3781 caatgttacc aacataaatc atcataaggc ataaagattt ggttattatg caactaaaat
     3841 tatagctaaa aaaatgtaaa tgcaagaaaa aagcagcttt tttaagctgc tttcccgcaa
     3901 aaaaatttaa aagcctagct gccaagcgtt gccaccatta cagctttaat ggtgtgcacc
     3961 ctattctcag cctcatcaaa aacaattgag gcatccgact caaaaacctc atcggtaacc
     4021 tccatggatt ctaatccgaa tttcttgaaa atatcttcgc cgactgcagt ctcgcggttg
     4081 tggaaggagg gcaggcagtg catgaacttg cagtttggat tgccggtgag ctccatggcc
     4141 ttgcggttaa cctggtaggg ttttaagagc ttaattcgct cgtcccaaac agaatcggct
     4201 tcacccatgg aaacccaaac atcggtgtag aggaaatcgc accctttaac gccctcttct
     4261 accttttcgg taagggttat ccgggctccg gtggttttgg caatctccat gcaggttttt
     4321 accagttctt cttttggcca gcaggcctta ggcgcacatg ccctgaaatc cattcccatt
     4381 ttagctgccc caaccataag cgagtttccc atgttgtttc tggcatcgcc catgtagcag
     4441 aaggtaactt tgctcagcgg tttgctgcta tgctcgcgca tggtaagaaa atcggccaga
     4501 atttgagttg ggtggtactc agtggttagg ccgttccata caggaacacc cgcatacttg
     4561 gcaagagttt cgacaatttc ctgggagtag ccgcggtact caattccgtc gtacattctg
     4621 cccagcaccc tggcggtatc cttcatggat tcctttttcc ccatttgcga acctgaaggt
     4681 ccaaggtagg ttacatgggc accctgatcg taggctgcca cctcgaaggc gcaccgggtt
     4741 cgggttgagt ccttctcaaa taggatcaca atgtttttcc cctttagctg ctgctgttcg
     4801 gttccggcgt actttgcctt tttaaggtcg gccgataggt cgagtagaaa ctgaatttct
     4861 ttcggagtaa aatcaagaag ttttaaaaaa ctccgattcc ttaggttgaa tgacatactc
     4921 ttaagttttt atgctgtttt acaatttatg ataagcttaa aatgcactgt tgaaatcatg
     4981 gtttcaggtt cgcccgttca gctgcattcc tggagggcta tgccttctct aaaagtctta
     5041 tagctcgaac ggtatgctta ccgggttagc ttgataccta gtcctcgtag gccatggtta
     5101 ttttagttcc gtagctcata tcctccaact tggtggcttc ggttatgaca ctcattttgc
     5161 ctccattctc gatgaagttc aaacaggcac gtattttagg tgccatgttg ccctcgccaa
     5221 acatcccctc ctggaggtat ctggttgcat cggcatggtt gaggaattcc aacttcttct
     5281 cgttgggttt cttgtagttg aggaacacaa atggcacatc ggttagaatg tagaactcgt
     5341 cagccttgat ttcggcatcc attagcgctg atgcggaatc cttgtcaata accgcctcag
     5401 caggcctcac atttttctcc tcatcaatgt aaacaggcac accacctcct cctgaggcaa
     5461 taacaattat tccctgatcg accagctggt ctataacctt gatattcaaa atcccctttg
     5521 gctttgggga tggaactacc ctgcgccaac cactacccgt atccttcacc tcttccttaa
     5581 aaatccatcc tttttgtgag gccagggcat cggcttgagc cttgtcgtat attcgcccca
     5641 cacgcttggt tgggttgcta aaggctgggt cgttcttgtc aacaattacc ggtgttacca
     5701 gacagcaaac ctccttatca atgccgtgat ttttcagcac gttacggagc atgcgttcaa
     5761 tcatgtatcc aattcctccc tgcgaatcgg caacgcatac atccaggggc atttggggaa
     5821 tgccgtatag ctgctctccc gcatcgttcc gcatgaggat attgcccacc tgagggccat
     5881 ttccatggct aatgacaaga ttgtagcctt ccttaatcag ataaaccagg ttctccagtg
     5941 tttcggttgt gttttgctcc tgttcttcta ttgtcccaac ctgattcccc cttaggaggg
     6001 cattacctcc taaagcaaca actgctaatt tgctcatata caaattatta atataatgtt
     6061 gatttaaaat tgcaaagcta ttaaaatttt tcaaaggtat tttaccctgc catcttcagg
     6121 cttgcttaat caatagtttt gcagtatgtt taataacata ttttttattg ctgcttttca
     6181 tcacctcttt tttagaaagg ttcgagattg ttttctccga atgctgactc gctggaagtg
     6241 aaaaaagggg gattagccag ctagcttgcc ttttgggaca tggcctagtt tattattgca
     6301 atagttgtat atatggcggc tattttacta cttttatggt ttgttaagga tgatgcatat
     6361 gaggaagcca attgccggtt tggtcatttt gtctgctttt attctttgtt catgtggcgg
     6421 tagggttaaa aacaatagca tttcagaggg tgttattact tatgacatca cctacgatag
     6481 cgcaattcag agccggtatg atgcatggct gctaccttcc gagttgatag ttaggtttaa
     6541 cagcaaccat tccataaatt ccattgaggc tctagggggc gccatttcca tttcaattat
     6601 taagaatcct gaacgtaatg aattttcgac actgcttaag atttttaata aaaaacttta
     6661 ccacagcgaa cccatagaac aggagcgtta cccggcactt tactcccgaa tgcccgcggt
     6721 aaagttgatt tcggagggtg aaacatgcga atttttgggt tatagctgca ctaaagttaa
     6781 aggctacttt gccgataatc ccgattttcg attcgaaatt atttacacca gccagattca
     6841 aatcgaaaac ccaaatgccc acacaccctt tgaggaggta aagggtaccc tgttgcagtt
     6901 taacatcagg ctgaataacc ttaccatgca cgccaccgca aggtcggtaa agtcggagca
     6961 tgtcccgctt gagctgtttg ctcctccatc ggactatgtt gttaccgatt cacaaacgat
     7021 agccaattta atctacacgc ttcagcagta agtttgtaaa tctaccggta agagttgaat
     7081 gtaggttgtt ccccaaaagt acattaacct ttggattttg tcggatttta ttcattactt
     7141 ttgcatcttg tcttaatgat gtaggggata atgcaagttg gacaactgca accagggtgc
     7201 ttaaaccgta aggagattct gccagtcatg aattctgtcc ttttgcccca gctcttgctt
     7261 taaaaataaa cgattcaggg atggcatcag atagcaaccg gccaaatgta aaggaaaaga
     7321 acaacgccac aaaggagggg caggacaacg ggaaaaataa acttctccgg gacgaaaggg
     7381 taaggtttac cattgggtta ttcttgctgc tactgggtgt ttactttctt atttccctga
     7441 tttcatacct gttcacgtgg aaagttgacc agagtctgga gtggcaaagc cttttttcca
     7501 aagccgaata cgaggtgaag aactggaccg ggaggtttgg agcggttata tcggctcagt
     7561 ttatgaacca ctggtttggc ctctcttcgc ttgcgctgcc atctattatc atagtttttg
     7621 gacttaagct tcttaagctg tacagaaaaa atatctggcg gtttatcttt aatatcttca
     7681 taggaacact actatgctcg ctagtgctgg gctttttatt tgaaaccgct aagggatttc
     7741 ttggtagcgg gctaggggga gcgcaaggac tttttgtgtc gaaatggctg tcggcattgc
     7801 tgggtaaggt tggaacctct tttttgctgt tacttctggt tattgcctac accattttta
     7861 tcaagccttc aacgctacac tggttcagaa gaaccatcag gggattactt attagaatga
     7921 ggcgcccggt ttcccgaaaa aatgataaaa ggcaaaataa aaaagaaaaa actgctgagg
     7981 agttggctgg acaagttgac tctgaagttc tatctaataa caccgatgcc gctgttgagg
     8041 ttactttgga aaagttcatt cccgagccta attcagaatt ggttgatgct gagccattcg
     8101 agcttatcga agaggagaca gctggcacta acagttcaaa tgcagaaaat actcccgaaa
     8161 aatccgatgt ggagttcacc attgaaaaaa tcaacgag
//
LOCUS       DAOOQX010000008         8037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_11097_length_8037_cov_2.859183, whole genome shotgun sequence.
ACCESSION   DAOOQX010000008 DAOOQX010000000
VERSION     DAOOQX010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11097_length_8037_cov_2.859183"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="PLX53_00300"
     CDS             <1..1093
                     /locus_tag="PLX53_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOY71551.1"
                     /translation="GSKVTIRVEVKSPEKNMLYISRIDFGKTTPIDSAKISSGESIKK
                     FRLPQGSEPTFYTISLKKGGALTLLASRDEKISVKFDVQNMDSYEVVGSKESLKVKEI
                     AESFRKSKQTVDRLRKEYIKAQTDAEREAIGRQFQAEIDSQRASCSRFIWANPMSKAS
                     IMALYQKYNDDLYLFDRAEDLTLLKMVASAWRALYPESDYTRGMLEDIGRIEKIITNS
                     KINKLISEAEPSIPDLDIPDKNGTFVKLSSLKGKVILLDFFTASNTSSLLDNRELMNI
                     YNTFKNRGFEVYQVSMDADKNEWLNYLSNTRVTWISVREVDPTHSAAALLYNVTQLPT
                     NYLIDKNFNIIDKNIYGERLKEKLNVLLR"
     assembly_gap    1320..1329
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1330..1990
                     /locus_tag="PLX53_00305"
     CDS             <1330..1990
                     /locus_tag="PLX53_00305"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOY71552.1"
                     /translation="KGYTRFMGRIAQLTDRGIPVHFFTGNHDVWMFNYFKEELGVEIH
                     HKPIEKVLYGHKVLMAHGDGLGPGDIGYKLLKRIFTSTTLQWLFARLHPNFSLWFGNQ
                     WSVSSRYSKEITHVFRGDDEQITAYARQVLDSKHFDIFMFGHWHSPTIFPLTQDSNLV
                     VLGDWLVSNTYAVLDGDDFWLMKYFPDKPDVPLGHIKLSHVPEAPVLPLHDQFNRINK
                     Y"
     gene            2051..2638
                     /locus_tag="PLX53_00310"
     CDS             2051..2638
                     /locus_tag="PLX53_00310"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HOY71553.1"
                     /translation="MNFLAHLYLSGCNPEVRLGGFIGDYVKGSKFTHFTPDIQKGIKL
                     HRAIDSVTDSHPSTRACSAIFKPVYKRYSGVVSDVVFDHVLAKEWSRYSESNLNEFTQ
                     IFYTQMFQRYQLLPPTVQQFLPNMIRTNRLSSYQTSEGVREALNIMSLRTSLPNNTEY
                     AIVQLKTHYNLFFDYFHPLFNDLMQLATREFGINL"
     gene            complement(2628..4055)
                     /gene="rodA"
                     /locus_tag="PLX53_00315"
     CDS             complement(2628..4055)
                     /gene="rodA"
                     /locus_tag="PLX53_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOY71554.1"
                     /translation="MQRKSNLVSNLDWGTIVLYVILVLMGWMNIYAAVYSEEHNSILD
                     FSQRYGKQLIWITFAFFLGVVVLLSNGRIYSVFAYIIYGLVILLLLSTLVFGRVVNAS
                     KSWIPLGSFGIQPAEFAKFATALALARLMSPYEFKLNTFWSYVKVGLIIAIPAGIILL
                     QNDTGTALVFGSFVLVLYREGLPWWVLGVLFLLVALFISTLVFSTPVIIVALYVTTVL
                     IYMLQRGKFKFAISLTAGLTGLGFLLYLASQTFNIQVKTDYFYIIPVGMGIVIATLYA
                     FFSRTKMIYGLVLFLCVTVGFSLSTEYVFENVLDVHHQKRINDLLGIESDPLGWGYNV
                     NQSKIAIGSGGFWGKGYLSGTQTKFNFVPEQSTDFIFCTVGEEWGFVGSLVVLALFAW
                     LLLRLIRIAERQRESFSRIYGYGVVSILFFHIAVNIGMAVGLLPVIGIPLPFFSYGGS
                     SLWAFTILVFILLRLDADRYGTFKG"
     gene            complement(4039..5892)
                     /gene="mrdA"
                     /locus_tag="PLX53_00320"
     CDS             complement(4039..5892)
                     /gene="mrdA"
                     /locus_tag="PLX53_00320"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOY71555.1"
                     /translation="MRFNFQADKRFTITGLIIIVFIIIAGKLFYIQVIDRSYQLSASS
                     NVLRYVTKYPARGLIFDRNGNLLVSNKVVYDLMMVKKQVVPFDTMAFASLLNITTDHV
                     RDAFVQASRQKGYSPRKSVVLLKQLPAEACAMLQEKLYLFPGFELLPRTVRLYDREVA
                     SHVLGYIGEVDEEIISRNPYYQLGDYIGISGLEKSYEEYLRGKKGVSVYMVDVYNRIK
                     GSYEDGKYDSAAVEGQNLICTLDADLQEYGEKLMQNKIGSIVAIEPRTGEVLAMVSSP
                     TYNPGLLVGRERADNFRTLQNDSLKPIFNRSIMALYPPGSTFKVVNALIGLQEGCVYP
                     QTTYSCHGGYTVGRGVGCHIHPSPLNLTQAIQMSCNTYFCHVFRNILDKPEFGSVEKG
                     FTVWKNHVESFGYGNKLNIDLPNEQKGIVPTVAFYDKYFRKGGWNSLTILSLSIGQGE
                     LAATPLQMANLVTIIANRGFYLDPHVVRAVGDSMVRNSNVPEKHYTTIDTQWFDYIVE
                     GMDMAVNGAPGSGSTARIAALPDIRICGKTGTSQNPHGADHSVFFAFAPKDDPKIAIS
                     VYVENAGFGATWAAPIASLMIEKYLRDSISRPYLEDYILQANLLDRRAKKK"
     gene            complement(5899..6408)
                     /gene="mreD"
                     /locus_tag="PLX53_00325"
     CDS             complement(5899..6408)
                     /gene="mreD"
                     /locus_tag="PLX53_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOY71556.1"
                     /translation="MYKDYLRYILIFILLLLIQLLVLNNINLGGYINPYVYLLFILLL
                     PYGTPRWVLLLVGFFTGLVVDYFSNTLGLHASATLILAFVRPSIMSGLRFSNNINERS
                     MLNISLTGTEWFIRYAALGIVIHHSTLFMLETFSFRFFHLTLLRILLSSLVSFVFIFF
                     LEVLLTRKK"
     gene            complement(6408..7226)
                     /gene="mreC"
                     /locus_tag="PLX53_00330"
     CDS             complement(6408..7226)
                     /gene="mreC"
                     /locus_tag="PLX53_00330"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOY71557.1"
                     /translation="MNSFIRLLQRFSYIILFAVLLAVSLSMYINTSYYQKAKVGAATG
                     VITGFFSEKLHNIRSYMDIKRDNVKLSLENVELRNEVERLREVIGKCADTAGYNLADT
                     SITLIRAMVVNNSVSHRQNFITINIGINDGAYQGMGVICDGGVVGVIAATSPNYSTVI
                     SLLNTTLKVSAKHAKSGVFGTLSWNGNNYQEVELTDIPLHVGISVGDTIATSGFSSIF
                     PKDIPIGVIKDFYKKDGSFYNIRVQLLTDFRALNYVYAVRVRNITEINQIEGSR"
     gene            complement(7416..>8037)
                     /locus_tag="PLX53_00335"
     CDS             complement(7416..>8037)
                     /locus_tag="PLX53_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOY71558.1"
                     /translation="IYEPMAAALGIGLDVLAPEGNMVVDIGGGTTEIAVIALGGIVCN
                     KSIRNAGDGFTSDIQAYMRHQHNVKIGERTAEDIKIKVGSALPDLENPPADLIVRGPN
                     LMTALPIEVPVSYQEIAYCLDKSLSKIESAIFNVLELTPPELYADIVEKGIYLAGGGA
                     LLRGLDKRLTEKINIPFRVVEDPLHAVARGTSIALKNVGKFPFLMR"
BASE COUNT         2370 a         1908 c         1736 g         2013 t
ORIGIN      
        1 tggcagcaag gttaccataa gggttgaggt gaagtcacca gaaaaaaaca tgctttacat
       61 aagccggatt gactttggta aaacaacccc aatagactca gctaaaatat catccggaga
      121 aagcattaaa aaatttaggt taccccaggg cagcgaaccc acattctaca ccatcagcct
      181 gaaaaaaggg ggagcactta ctctactcgc aagcagggat gaaaagattt cggttaagtt
      241 cgatgtccaa aacatggaca gctacgaggt ggtaggctca aaggaatcgt tgaaagttaa
      301 ggaaattgcc gaatcattta gaaaatcaaa gcaaaccgtt gacaggctta gaaaggagta
      361 catcaaagcc caaactgacg ccgaacgtga ggctattgga aggcagtttc aggcagaaat
      421 agactcgcaa agagccagct gcagcaggtt catctgggct aaccctatgt cgaaagccag
      481 cattatggct ctttaccaga aatataacga tgatctctac ttgtttgacc gggctgagga
      541 tttaaccctg ctcaaaatgg tagcctcagc atggcgggca ctctaccccg aatcggacta
      601 taccaggggt atgcttgagg atattggcag gattgagaaa attattacca actctaaaat
      661 aaacaagctg atttcggaag cagaaccatc tattcccgac ctggacatcc ctgataaaaa
      721 tggtaccttt gtaaagctat catcacttaa aggaaaagta attcttcttg actttttcac
      781 cgcatccaac acctcaagcc tactcgacaa ccgtgaattg atgaatattt ataacacctt
      841 taaaaatagg ggttttgagg tttaccaggt ttccatggat gccgataaaa acgaatggct
      901 taactacctg tcaaataccc gagtaacatg gatttcggtc agggaagttg atcctacaca
      961 ttcagctgct gccctacttt acaacgttac acagctgcca acaaactacc tgattgacaa
     1021 gaacttcaac attattgata aaaacatata cggtgaaaga cttaaggaga agctgaatgt
     1081 ccttctaagg taggctaagc ctaaacgcca agtaaccatt cagcatgtag agatgttcaa
     1141 aaacacttat ttaagccggt gaatcagaat cgaaaaatat actttgcctc cgaccttcac
     1201 cttgggcatc caaatcttag agatagcctc ccccgtgaga agcttttcat atcgtggctc
     1261 gatagtattg atgctgatat tgaggagctc tacctgctgg gtgacatttt tgacttctgn
     1321 nnnnnnnnna aagggatata cccgtttcat gggacgtata gctcagctca ccgatagggg
     1381 gataccggta catttcttca ccggaaacca cgatgtttgg atgttcaact attttaaaga
     1441 ggagctaggt gtcgagattc accataaacc catagagaag gtgctttacg ggcataaggt
     1501 gctgatggcg catggtgacg gccttggacc gggagatata ggctacaagc tgttgaaacg
     1561 catttttacc agcaccaccc ttcagtggct ttttgccagg ctacacccaa acttttcact
     1621 ttggtttggg aaccagtgga gcgttagcag ccgctactca aaagagataa cccatgtttt
     1681 cagaggcgac gacgagcaaa taaccgctta cgcccggcag gttttggata gcaagcattt
     1741 tgacattttc atgtttggtc actggcattc accaactatt tttcccctta cgcaggattc
     1801 caacctggtt gtgctaggcg actggctggt tagcaacact tatgccgtgc tcgatggtga
     1861 cgatttttgg cttatgaagt atttccccga taaacccgat gtgcccttag ggcatatcaa
     1921 gctcagccat gttcccgaag caccagtttt gccgcttcat gatcaattta acagaattaa
     1981 taaatattag gtagatcggc ttattcagcg cttccaccag tgcaattaac caaaagcaaa
     2041 catcaaccct gtgaattttc tagcccatct ctacctttcg ggttgtaatc ctgaagtcag
     2101 attgggcggc ttcattggcg actatgtaaa aggtagtaaa tttacccact tcactcccga
     2161 tattcaaaag ggtattaaac ttcatagggc aatagacagt gttaccgata gccacccttc
     2221 cacccgagcc tgttcggcaa tttttaaacc agtgtataag cgctactcgg gtgtggtgag
     2281 tgatgtagtt tttgaccatg ttttggcaaa agagtggagc cgctactctg aaagtaacct
     2341 gaatgaattc acacaaatct tctacaccca aatgtttcag cgctaccagc ttctcccccc
     2401 taccgttcag caattcctcc ccaacatgat acgcaccaac cggctttcga gctaccagac
     2461 aagtgaaggg gttcgggaag cactcaacat tatgagcctc cgaacctcac ttcccaacaa
     2521 cacggagtac gccattgtgc agctgaaaac tcactacaac ctatttttcg attattttca
     2581 cccgcttttc aacgatttaa tgcagcttgc gaccagggag ttcggcatca acctttaaat
     2641 gttccatacc tgtcggcatc aagccttagc aggataaaaa ccaggatagt aaaagcccac
     2701 aacgatgagc ctccgtagct aaaaaaaggt aaagggattc ctataacggg aagtagccca
     2761 actgccatcc caatgtttac tgcaatatga aaaaatagga tggacactac cccgtacccg
     2821 tatatcctag aaaaactctc acgttgtcgt tcggcaatcc tgataagacg caagagcagc
     2881 catgcgaaaa gcgcaagaac taccaaagaa cctacaaaac cccactcctc acccaccgtg
     2941 cagaaaataa aatcggtact ttgctcagga acaaagttga actttgtttg ggttcccgaa
     3001 aggtatccct ttccccaaaa cccacccgaa ccaatggcaa ttttggattg gttaacattg
     3061 tagccccatc ccagcggatc ggattcaatg ccaagcaaat cattaattcg cttctgatgg
     3121 tgaacgtcga gaacattctc aaaaacatac tcggtggaaa gcgaaaaacc tactgtaacg
     3181 cataggaaaa gcactaaccc gtaaatcatt ttcgtcctcg aaaagaaagc atataacgtt
     3241 gctatgacaa tacccatgcc tacagggata atataaaaat aatcggtttt tacttgtatg
     3301 ttgaatgttt gtgaggcaag gtaaagtaga aagcccaaac cggtaaggcc tgctgttagg
     3361 cttattgcaa atttaaactt cccccgttgc agcatgtata ttaaaacggt agtaacatac
     3421 agcgcaacta taataaccgg cgtgctgaat accagggttg aaataaaaag cgcaaccagc
     3481 aaaaaaagca ctcccaacac ccaccatggc aatccctcgc ggtagagcac aagcacaaaa
     3541 gaaccgaaaa ccaatgccgt tccggtatca ttctgtagca gaataatacc ggcaggaata
     3601 gcaatgatta aaccaacttt aacgtatgac caaaatgtat ttagcttgaa ctcgtatggg
     3661 ctcattagcc tggcgagagc aagtgcagtg gcaaacttgg caaactccgc aggctgtatt
     3721 ccaaatgatc cgagtggaat ccatgatttt gaggcgttaa ccaccctgcc aaacaccagg
     3781 gtggaaagga gcaaaagaat aaccagcccg tagatgatgt aggcaaaaac ggaatatatc
     3841 cttccgttgc ttaacaaaac aacgactccc aggaaaaagg caaaagtaat ccaaatcaac
     3901 tgcttgccat agcgctggct aaaatcgagt atgctgttgt gctcctcgct gtaaactgcg
     3961 gcatagatgt tcatccaccc catcaacaca aggatgacgt aaagcacgat ggttccccaa
     4021 tcgaggttgc ttaccaggtt actttttctt tgcacggcgg tcgagtaggt ttgcttgaag
     4081 tatatagtct tcgagatagg gacgggatat agagtcccgt aggtactttt caatcattag
     4141 gctagcaatt ggcgcagccc aagttgcacc aaacccggca ttttccacgt acaccgaaat
     4201 ggcaatttta ggatcatctt tgggagcaaa ggcaaaaaat acggagtggt cggctccatg
     4261 tggattttgt gatgttcccg ttttcccgca aatgcgaata tcggggagag cagcaattcg
     4321 ggcggtactt ccgctcccgg gggctccgtt aacggccata tccattccct caactatata
     4381 gtcgaaccac tgggtgtcta ttgtggtgta gtgcttttcg ggaacatttg agtttctcac
     4441 catgctgtca cctacagctc tcaccacatg gggatcaagg taaaaccccc tgttggcaat
     4501 aatggttacc aggtttgcca tttgcaaggg tgttgctgcc agctctccct gcccaatgga
     4561 aagcgagagg atggtaaggg aattccagcc gccttttcta aagtacttgt cgtagaacgc
     4621 aactgtcgga acaatccctt tctgctcgtt gggtaaatca atattcagct tattaccgta
     4681 accaaatgat tcgacatgat ttttccagac cgtaaaaccc ttttcgactg atccaaattc
     4741 gggcttatcg agtatgttcc ggaacacatg gcagaaatag gtgttacacg acatctggat
     4801 ggcctgagtt agattcaatg gagaggggtg aatgtggcat cccacacctc gcccaacggt
     4861 gtatcctcca tggcagctat aggtggtttg cgggtaaacg caaccttcct gtagcccaat
     4921 aagcgcattc acaaccttaa aggtagaacc cggtgggtaa agggccatga tggagcggtt
     4981 aaaaattggt ttgagcgaat cgttttggag ggtcctgaag ttatcggcac gctcgcggcc
     5041 aacaagtaat cctggattat acgtagggct tgaaaccatg gccagcacct cgcctgttct
     5101 gggctcaata gccacaatgc tgcctatctt attctgcatc aacttttcgc catactcctg
     5161 aaggtcggca tccaaagtgc aaatcagatt ttgcccctct acggcagcag aatcgtattt
     5221 tccatcctca tacgagccct tgatgcggtt gtaaacgtca accatgtaaa cgctaacacc
     5281 ctttttccca cggaggtact cctcatacga tttttccaaa ccgctgatac caatataatc
     5341 gcccagctgg tagtagggat ttcttgaaat aatttcctcg tcaacctcgc caatgtatcc
     5401 cagaacatga cttgctactt ccctgtcgta aagcctaaca gtacgtggca gcagctcaaa
     5461 tcccggaaac aggtaaagct tttcctgaag cattgcacat gcctcggccg gaagctgctt
     5521 aagcagcaca accgatttgc gcggggagta ccctttttgg cgggaagcct gtacaaacgc
     5581 atcccttaca tggtcggtag taatgttcaa cagggaggca aatgccatcg tatcaaaggg
     5641 tacaacctgc ttcttcacca tcatcaggtc gtaaaccacc ttgttcgaaa ccagcaggtt
     5701 tccgttccgg tcgaatatca acccacgagc cggatacttg gttacatacc ttaacacgtt
     5761 actgcttgcc gaaagctggt acgacctgtc tatcacctga atatagaata gcttccccgc
     5821 tatgatgata aacactatga tgattagccc ggtaatggtg aatcgtttat cagcctgaaa
     5881 attgaacctc atagcccatt acttttttcg ggtgagtaaa acttcaagga agaaaataaa
     5941 cacaaacgaa accaggctgc taagtaggat tctcagtagg gtgaggtgaa aaaagcggaa
     6001 gctgaaggtt tcaagcatga atagggtaga atgatgaata actattccca gtgctgcata
     6061 gcgaataaac cactcggtgc ctgtaaggct tatgttgagc atgctccgct cgttgatgtt
     6121 attgctaaac cttagaccag acattatcga cgggcggaca aaagcaagta ttagcgtagc
     6181 cgaagcgtga agtccaaggg tatttgaaaa gtaatcgaca accaatcccg taaaaaagcc
     6241 tacaagcagc aagacccagc ggggagtgcc atagggtagc aaaagtatga ataacaggta
     6301 aacgtaaggg ttgatatacc ctcccaggtt aatattatta agtaccagga gctgaatcag
     6361 aagcaacagg ataaagatca gaatgtatcg caagtaatcc ttgtacatca cctactcccc
     6421 tcaatttggt taatctccgt aatgttccta accctaactg cgtacacgta gttaagcgcc
     6481 cgaaaatcgg taagcagctg aacacgaatg ttgtagaagc taccatcctt cttgtaaaaa
     6541 tccttgataa caccaatggg tatatccttt ggaaaaatgg atgagaaacc gctagtggca
     6601 atggtatcgc caacagaaat gccaacatgc aggggtatat cggttagctc aacctcctgg
     6661 taattattac cattccatga cagggtacca aaaacacccg acttggcatg cttggcagaa
     6721 accttaaggg ttgtattaag cagcgatata accgtgctgt agttgggcga tgttgccgct
     6781 ataaccccta cgactcctcc atcgcaaatc acacccatgc cctgataagc gccatcgttt
     6841 ataccaatgt taattgttat aaagttctga cggtgggata cggagttgtt cacaaccatt
     6901 gccctaatca gggtaatgct ggtgtcggca aggttgtagc ctgccgtgtc ggcacatttc
     6961 ccgatgactt ctctaagtct ttcaacctcg tttcgaagct caacattctc aagcgatagc
     7021 ttaacattat cccttttaat atccatgtag ctacgaatat tgtggagctt ctcagagaag
     7081 aaacctgtaa taaccccggt tgccgcacca acttttgcct tctgatagta ggaagtattg
     7141 atgtacattg aaagtgaaac ggccagtagc accgcaaaaa gaatgatata cgaaaatcgt
     7201 tgaagtagcc gaataaagct gttcatcctc gatactctta attaccagcg tcaaaacatc
     7261 aggttgaaaa tattaaaaaa cgctccaaca accatttaca agggtgctat caggtgcttc
     7321 atttttttgt gtagccatcc agacgagctg gatatagaaa tatttttcct aacggagcca
     7381 cacggggctt aatgattttt aaactctgtt accctttaac gcatcaggaa tgggaatttg
     7441 ccaacatttt tcagtgcaat gctggtaccc ctagctacag catgaagcgg gtcctccaca
     7501 accctaaagg gtatgttaat tttttcggta agccttttat cgaggcctct aagcagcgca
     7561 cctccacctg ccaggtaaat tcccttctca acaatatcgg cgtaaagttc aggaggtgtt
     7621 agctcaagca cgttgaatat tgccgattca atttttgaaa gcgatttgtc cagacagtag
     7681 gcaatttcct gatacgaaac cggaacctca ataggcaggg cagtcataag gttggggcct
     7741 cggacaatca ggtcagcagg aggattctcc aagtcgggaa gtgcagaacc gacctttatt
     7801 ttaatatcct cggcagtgcg ttcgccaatt ttaacattat gctggtgcct catgtaggct
     7861 tgtatgtcgg atgtaaaacc gtcaccggca ttccgaatgg atttgttgca aacaatacca
     7921 cccagggcaa taacggcaat ctcggttgtt cccccaccta tgtccaccac catgttccct
     7981 tcgggagcaa gcacatcaag gccaataccc agtgcagcag ccatgggttc atagata
//
LOCUS       DAOOQX010000009         7926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_11288_length_7926_cov_3.137721, whole genome shotgun sequence.
ACCESSION   DAOOQX010000009 DAOOQX010000000
VERSION     DAOOQX010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11288_length_7926_cov_3.137721"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PLX53_00340"
     CDS             <1..944
                     /locus_tag="PLX53_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOY71559.1"
                     /translation="ASVSTSLAEIMGGAIALQMLFGIPIKIGAVLVLAFVLVMLFTNS
                     YRAIEKWIIAFVSIIGLSFIYELSLVNIDWPEAIKSSVTPSFPEGSMLVIMSVLGAVV
                     MPHNLFLHSEVIQSRQWNLQDDRVIRRQLDYEFFDTLFSMLVGWAINSSMILLAATTF
                     HKTQTPVSELSQAQNMLIPLLGSSAADIFAVALLLAGISSTITSGMAAGSIFAGIFNE
                     PYDVKDNHSKLGILLSLVAAVLVIFAIDNPFKALLISQMVLSIQLPFTIFTQVRLTSS
                     EKVMGKYKNSPFTKWTLYGLGAVVTLLNLMLFVSFLG"
     gene            complement(1009..1404)
                     /locus_tag="PLX53_00345"
     CDS             complement(1009..1404)
                     /locus_tag="PLX53_00345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOY71560.1"
                     /translation="MASIFSKIVNGEIPSYRVAEDEKYFAFLDINPLAEGHTLVIPKQ
                     EVDYLFNLDQETLSGLVLFAQRVAKAIESVIPCKRIGVAVLGLEVPHAHIHLIPINSE
                     SDIDFHKPKLKLNPEQLDAIAQKIATKFE"
     gene            complement(1417..1890)
                     /gene="greA"
                     /locus_tag="PLX53_00350"
     CDS             complement(1417..1890)
                     /gene="greA"
                     /locus_tag="PLX53_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOY71561.1"
                     /translation="MSKVNYLTEEGLEKLRKELDHLKSVERPAISKMIAEARDKGDLS
                     ENAEYDAAKEAQGMLELRISKLEDLVANARVIDESKIDTERVQILNRVKLKNLKTNAV
                     VEYMLVSESEANLRENKISIATPIAKALIGKKIGDIVEVQVPSGLMKFEILSISL"
     gene            complement(2000..2647)
                     /locus_tag="PLX53_00355"
     CDS             complement(2000..2647)
                     /locus_tag="PLX53_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71562.1"
                     /translation="MDGGDILFIIIVTILGIISSVSRKKKEAAKRAMKQTEAEEPKNY
                     SEGQPVEIPENNNEDIYTWPQQSVSEFPSNDTMQDFTSNSNPRNEGKSVLSGWDNMEG
                     EVKSVEYEMPSVQVSEEELYAPKKYEELDKIPTSSGSSLLDQPVSPYETTYEGLASSY
                     QPIAFEEQEEESKAPDMIFEYEKVLEQESNQAGILDDFDPAKAVIYSEVINKKYF"
     gene            2815..3246
                     /locus_tag="PLX53_00360"
     CDS             2815..3246
                     /locus_tag="PLX53_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71563.1"
                     /translation="MRAKIAKLLVIAASFFLPSSCGEDEPEIVPDVPVHIEFYLNEPF
                     YNNITVPMGAVKIPNEGYNNNGIIVFRLNQEEIVAFDATCPQHIAVNASVALDENGSG
                     TATCPHCHTVYYFYNNGYPTKGYKLKSYQVSFRGDLVGIYN"
     gene            3410..5311
                     /locus_tag="PLX53_00365"
     CDS             3410..5311
                     /locus_tag="PLX53_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOY71564.1"
                     /translation="MIPPVKHAPRWLVFVIDLCICLVSITLSYYVRFEFRIGSEQVNA
                     LKLVAPLVLFVRALAFISFRTYAGLIRYTSLKDAERIFMVTTSSTIVLFTINFIVYSK
                     SGQFLIPNSILLIDFCGTNILMIGSRLFYKTLYYTIKNVDIEAENIVIVGIEQFAASA
                     KRAIDKDNMAGLKVVAFIDPNCKQEGNSIEDVRIYNTSKIEFLVQKYGVSRILIAQKN
                     LDPVKKNKIIDKCLQLNVKVQVIPDATAWINGELNTSQLKSINIEDLLERPPIVLDKR
                     RIQNYIKNKVVLVTGAAGSIGSEMVRQISKFSPKKIVLFDQAETPLYDLELELKENLH
                     FYDFESVIGSVTNDYRVNKLFDVFKPNVVFHAAAYKHVPMMENNPTEAVFNNVLGTKL
                     IAETAVRYGVEKFVMISTDKAVNPTNVMGASKRIAEIFTQSLSCQVDTNFITTRFGNV
                     LGSNGSVIPRFRKQIEKGGPVTITHPEVTRFFMTIPEACQLVLEAGAMGNGGEIFIFD
                     MGEPVKILDLAKKMIKLSGLTLGKDIQINFTGLRPGEKLYEELLNNTENTIPTYHPKI
                     MIAKVPDLIPENVIPKINRLIETLPEHNNFKVVSLMKDIVPEFISRNSIYEDIDRQKK
                     YIVNDYKTA"
     gene            5292..5744
                     /locus_tag="PLX53_00370"
     CDS             5292..5744
                     /locus_tag="PLX53_00370"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOY71565.1"
                     /translation="MTIKPHSYFHSTPIQIRVNDIDPLNHVNNSVYQQYFDLGKIAYF
                     NRVLGEHLNLETEGLVLANITIDYLKPITLYDSIEVFTRIYEMGTKSLKMGQEIVNRV
                     SKEVYTTSKSVLVCFSNSSGLSIPIPLLWRERIADFEKEGLIHGVNLQ"
     gene            complement(5718..6248)
                     /gene="rimM"
                     /locus_tag="PLX53_00375"
     CDS             complement(5718..6248)
                     /gene="rimM"
                     /locus_tag="PLX53_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOY71566.1"
                     /translation="MTDTQLVEVGFIQRTHGVKGEIQISVASSAGVLPEELELVFLDI
                     EGIPVPFFIQGIVAISDEILIVKFDEVDSVEEAAELVGLRVLLPCGCLSNDDGGVPFS
                     SLVGYRLFSTKNEELGIITRYEEFAYNALFAVTAKSGKELLIPAAENLISRIDDAERI
                     VWMDIPQGLLEVNSMD"
     gene            complement(6255..6809)
                     /locus_tag="PLX53_00380"
     CDS             complement(6255..6809)
                     /locus_tag="PLX53_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOY71567.1"
                     /translation="MPVKIRLSRHGRKKNPYYHIVVADSRAPRDGRYIERIGSYNPLT
                     NPATIELNFDRALDWLQKGAQPTDTCRAILSYKGVLYKNHLLIGVKKGAITMEQAEAK
                     FDTWLKEKEGKIKAKIDFVENSNAKRKNERLEAEAKVREARAQAVAKKRAAEAEKEAK
                     KSSENSAQDAVEANADTEAGSSEE"
     gene            7037..>7926
                     /locus_tag="PLX53_00385"
     CDS             7037..>7926
                     /locus_tag="PLX53_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOY71568.1"
                     /translation="MPFNRDKVEQWSLAYWLLKCHENFFFKLYFRTEIVGIEKLPDNA
                     TLIFAPNHQNTLMDALAILTLKRDFQPIFLARADIFKKPFLSKILTFLKIMPVYRSRD
                     GFENLLLNDRIFQKTLDVLQNRNGLVVLPEGSHEGVKKLRQLKKGIARIAFQAEDAAN
                     GKLGIQIVPVGLEYSNYVAFRSNLLIRLGEPIEVDRYLELYRQNRAQAYNALIAELEQ
                     RMRLEMINIEDENYTAYLTILELFSARYIKERKLPKSQNQKFQVDKKIISLLDRLKNT
                     NASEFEEIIKNANDYGRIISA"
BASE COUNT         2294 a         1839 c         1574 g         2219 t
ORIGIN      
        1 tggcatcagt ctcaacctcg ctggccgaga taatgggagg cgcaatagca ctacaaatgc
       61 tattcggcat acctatcaag attggcgcag tgcttgtttt ggcgtttgtg ctagtgatgc
      121 tattcaccaa tagctaccgg gcaattgaaa agtggattat agcatttgtc tccataatag
      181 gactatcgtt tatctacgag ttgagcctgg ttaatattga ctggcccgaa gccatcaaat
      241 cctccgtaac gccatcgttt cctgaaggct ccatgctcgt tatcatgagc gttctcgggg
      301 cagtagttat gccccacaac ctgtttctgc actccgaggt aattcaaagc cgacagtgga
      361 accttcagga cgacagggtg atacgaagac aactcgacta tgaatttttc gatacccttt
      421 tcagcatgct ggttggatgg gccataaaca gctccatgat actacttgct gcaaccactt
      481 ttcataaaac ccaaacgccg gtttcggagc taagtcaggc tcaaaacatg ttaataccat
      541 tacttggaag cagtgcagcc gatatatttg cagtagctct gctacttgca gggatatcct
      601 ctaccataac ctcggggatg gctgccggat caatattcgc aggaattttt aacgagcctt
      661 acgacgtaaa ggacaaccat tcaaaacttg gaattctgct ttcgctggta gctgctgtgc
      721 tggttatatt tgccatagat aaccctttta aagcactgct aatatcccaa atggtgctaa
      781 gcattcaact acccttcacg atattcaccc aggttaggct cacctcctcc gaaaaggtga
      841 tgggaaagta caaaaacagt ccctttacaa aatggacact ttacggtctt ggagccgtag
      901 taaccctttt aaacctaatg cttttcgtaa gctttctagg ataggttgaa gtattagcct
      961 gattgtcctg caaagaggca cggaatggaa gcccgctacc acacacagct attcaaattt
     1021 cgtggcaatt ttttgggcaa ttgcatcaag ctgttcagga ttcagcttta gctttggttt
     1081 atggaaatca atgtccgact cggaattaat tggtatgagg tgaatatgcg catgaggcac
     1141 ctcaagccct aaaaccgcta caccaatacg cttacatgga atcacgcttt caattgcctt
     1201 ggcaacccgc tgggcaaaaa ggactaatcc cgatagggtt tcctgatcga ggttgaaaag
     1261 gtaatcaacc tcctgtttgg gtattactaa ggtgtgccct tcagccaaag ggttaatgtc
     1321 aagaaaggca aaatattttt cgtcttcagc aaccctgtaa gagggaattt cgccattcac
     1381 aatttttgag aaaatggaag ccataacccg acaggattaa agtgatatgc taagaatttc
     1441 aaacttcatc agacccgaag ggacctgaac ctctactatg tcgcctattt ttttaccaat
     1501 cagcgcttta gctattggcg tggcgataga gatcttgttc tcgcgcaagt tagcctccga
     1561 ctccgatacc agcatgtact caactacagc atttgttttc agatttttaa gcttaacgcg
     1621 gttgagtatt tgcacgcgtt cggtatcaat cttcgactca tcaataaccc tggcattggc
     1681 aactaggtct tcaagctttg aaatacgaag ctccagcatg ccctgggcct ccttggctgc
     1741 atcatactcg gcattttccg acaaatcgcc cttgtccctt gcctcggcaa tcattttgga
     1801 aatggctggc ctctccacac tctttaggtg gtcgagctcc ttacgcagct tctcaaggcc
     1861 ttcttcggtt aagtagttca ctttggacat ttcaacctcc ttatacatct gaaataaaaa
     1921 agaattccga ttgctcggaa ctctcatctt ttgcaaatat aacaaatttt agaataaaaa
     1981 tctcggaatg aaatgaaagc taaaaatatt ttttattaat tacctcagag tagattactg
     2041 ccttggcggg atcaaaatca tccaaaatgc cagcttggtt cgattcctgc tcaagcactt
     2101 tttcatactc aaaaatcata tcgggggctt tagactcttc ctcttgctcc tcgaaagcaa
     2161 taggctgata gctagaagct agaccctcgt aggttgtctc gtagggacta acaggctggt
     2221 cgagtaggct gcttcccgaa gaagtgggaa ttttatcgag ctcttcatac tttttagggg
     2281 cgtacaactc ctcttcgctt acctgcactg acggcatctc atactccaca gatttaacct
     2341 caccctccat attgtcccag ccagacaaca cagacttccc ttcatttcgc ggatttgaat
     2401 tagaagtaaa atcctgcata gtgtcgttgg acggaaactc gcttactgat tgctgaggcc
     2461 atgtatatat gtcttcatta ttattttcag gtatttcaac tggttggcct tctgaatagt
     2521 ttttaggctc ctctgcttcg gtctgcttca tggcccgttt ggccgcctct tttttcttcc
     2581 tggaaaccga gctgataatt cctaaaatgg taacaatgat tatgaatagg atatctccac
     2641 catccatttc agcaaatatt tagttaacaa aaattcaact acacacacta acgtcatttc
     2701 cgttaaattg ttacgaacgg caatccgttg ctgtttttca ccatcaggtt taagccggta
     2761 ttataagtaa caaaaacaat tgtatttttg taatgtattt accggaataa aaccatgaga
     2821 gcaaagatag ctaaactttt agtaatagca gcatcctttt ttttgcctag cagctgtgga
     2881 gaggacgagc ccgaaatcgt ccccgatgtc ccagttcaca tagaattcta ccttaacgaa
     2941 cccttttaca acaacataac cgttcccatg ggcgcggtaa aaattcctaa tgagggttac
     3001 aacaacaacg ggattatagt attccgtcta aaccaggagg aaatagttgc ctttgatgct
     3061 acctgcccgc agcacattgc agtaaatgca tcggtggccc ttgatgaaaa cggctcagga
     3121 actgccacct gccctcattg ccatacggtt tactacttct acaataatgg ctacccaact
     3181 aaggggtata agctgaaatc ataccaagta tcattcaggg gtgatttggt gggaatatac
     3241 aactaacccg ctctccagga ctttcttccc gcattactct actacctaaa ataaaccaga
     3301 aaggtttgaa atttattcat cattctcatt ttaggaataa tttttgctta aaaaaatttt
     3361 ctaacaatac aatttttctc ctaaatttga atataaaatg ttacctaaca tgattccacc
     3421 cgtaaaacat gcaccccgct ggttggtttt cgtcattgat ttatgcatct gcctggtttc
     3481 cattactcta tcatactacg tacggtttga gtttcggatt ggaagcgagc aagttaatgc
     3541 cctaaagcta gttgctccgc tagtcctgtt tgtaagggcg cttgcattta tttcgtttcg
     3601 tacctacgca ggcttaattc ggtacaccag tttgaaagat gctgaaagga tattcatggt
     3661 aaccacttcc agcacaatag tactgtttac catcaacttt atagtttact ctaaatcggg
     3721 ccagttccta attccaaact ccatcctgct catagatttt tgcggcacca atattcttat
     3781 gattggctcc aggctattct acaaaacgct ttattatacc attaaaaatg ttgatattga
     3841 agccgaaaat attgtgattg taggcattga acaatttgcc gcttctgcaa aaagggcaat
     3901 tgacaaggac aacatggctg gtctaaaggt ggtggccttt attgacccaa actgcaagca
     3961 ggagggaaac tccattgagg atgtcaggat atacaacacc tcaaaaattg aattccttgt
     4021 tcaaaagtat ggcgtttcaa ggattttaat cgcccaaaaa aatcttgacc ctgtaaaaaa
     4081 gaacaaaata atagacaagt gcctgcagct caacgtaaag gttcaggtta tacccgatgc
     4141 taccgcatgg attaacggag agcttaacac cagccagcta aaaagcataa acattgaaga
     4201 ccttttagag cgccctccta ttgttcttga caagaggaga attcaaaact acataaagaa
     4261 caaggttgtg ctggtaacgg gtgcagcggg atcgataggg agcgaaatgg ttaggcagat
     4321 ctccaaattc agccctaaaa aaatagtgct cttcgaccag gccgaaactc cgctttacga
     4381 tcttgaactg gagttaaagg aaaacctgca cttctacgac tttgaatctg taattggaag
     4441 cgttaccaat gactataggg taaataaact ctttgatgtt tttaaaccca acgtggtttt
     4501 ccatgcagca gcctacaagc atgtccccat gatggagaat aaccctactg aggctgtttt
     4561 caacaacgtg ctaggcacaa agctaattgc cgaaaccgcc gtaaggtatg gagtagagaa
     4621 gttcgtgatg atttctacag acaaggcggt taaccccacc aacgttatgg gtgcaagcaa
     4681 gcgcatagct gaaatcttta cccaatcgct cagctgccag gttgatacca actttattac
     4741 cacgcgtttt ggcaacgtgc ttggttcaaa cggctcggtt ataccccgtt tcagaaaaca
     4801 aattgagaag ggtggtccgg ttactattac ccatcccgaa gttacgcgat tctttatgac
     4861 catcccggaa gcttgccagc ttgtgcttga agccggagcc atgggtaatg gaggcgaaat
     4921 ttttatattc gacatgggcg aaccggttaa gattcttgat ctggcaaaaa aaatgattaa
     4981 gctctcggga ctcaccctgg gcaaggatat acaaattaac ttcacggggc ttcgccctgg
     5041 cgaaaaactt tatgaggaac ttctaaacaa cactgaaaac accataccca cctatcaccc
     5101 caaaatcatg attgccaaag ttcctgatct gatccccgaa aatgtcattc cgaaaataaa
     5161 caggctaatt gaaactctac ccgaacacaa caacttcaag gttgtttcac tgatgaagga
     5221 tattgttccg gagttcatta gccgcaactc gatttacgag gatatcgatc gtcaaaaaaa
     5281 atatattgtt aatgactata aaaccgcata gctactttca ctcaaccccc attcagataa
     5341 gagttaacga tattgacccg ctgaaccacg tcaacaactc ggtttaccaa cagtacttcg
     5401 atctggggaa aattgcctac tttaataggg tgctgggaga acatctaaac ttggaaacag
     5461 agggcttggt gctggccaac attactattg attacctgaa acccataacc ctttacgata
     5521 gcatagaggt gtttaccaga atctacgaga tgggaactaa aagcctcaaa atgggacagg
     5581 agattgtgaa cagggtatca aaagaggttt acaccacgag caagtcggtg ctggtttgct
     5641 tcagcaactc cagtggactt tccatcccga tccctttact ttggagggag agaattgctg
     5701 attttgagaa agaaggccta atccatggag ttaacctcca gtaaaccttg aggtatatcc
     5761 atccaaacaa ttcgttcagc atcatctatt cttgaaatca aattttcagc ggcagggata
     5821 agcagctcct ttcccgattt tgccgtaacg gcaaaaaggg cattataggc aaactcctcg
     5881 tagcgggtaa taatgccaag ctcctcattt ttggtactga atagccggta acccactagg
     5941 gatgaaaaag gaacaccccc atcatcattt gataaacagc cacagggtaa aagaacacgg
     6001 aggccaacca gctcagcagc ctcctcaacc gaatcaactt cgtcaaactt aacaatcaaa
     6061 atctcgtcgg aaatagcaac tataccctga ataaaaaaag gaactggtat tccttcaata
     6121 tcaagaaata ccaattccaa ctcctcgggt aaaacgccag ctgaactcgc tactgaaatc
     6181 tggatttcac ctttaacacc atgagttctc tgaataaagc caacctcaac aagctgagta
     6241 tcggtcatgc atgactattc ttcactgctg ccagcctcag tatcagcatt tgcctccacg
     6301 gcatcctgag cagaattctc ggaagatttt ttagcttcct tttcagcctc ggcagctcgt
     6361 tttttagcaa ccgcttgagc tctagcctca cgtaccttgg cttcggcttc aagtcgttca
     6421 ttctttcgtt tagcgttgct gttttctacg aagtcaatct tcgccttaat cttgccttcc
     6481 ttctccttga gccacgtatc aaacttggct tctgcctgct ccatggttat tgcacccttt
     6541 tttacaccaa tgagcaggtg atttttataa agaacaccct tgtatgaaag gatagcccta
     6601 caggtgtcgg taggttgagc gcctttttgc agccagtcca atgccctatc aaaatttaat
     6661 tcaatagtag caggatttgt cagaggatta tatgaaccaa tcctctctat gtatctgcca
     6721 tcccgtggcg ccctgctatc cgccaccaca atgtggtagt agggattttt tttcctacca
     6781 tggcggctta gtctgatttt tacaggcata cttaacgctt ttttaattgg ttaaacctac
     6841 ttttttgccc cgcaaagata tgctttttta ttttattgct ctcatactac tgtaaatcat
     6901 tttttatgat actcgtcagc gctcaataat gcaaacgcta ctaatgccaa acacggatta
     6961 ctttaccata aactctatgg aacttataaa aaaaagtatc acatttgcag gctaacaatt
     7021 atatacccca aacgcaatgc cttttaaccg agataaagtt gagcaatggt ctctggcata
     7081 ctggttgctc aagtgccacg agaatttttt ctttaagctg tactttagaa ctgaaatagt
     7141 tgggattgag aaacttccag ataatgccac cctgattttt gcccccaacc accagaatac
     7201 ccttatggac gcccttgcca tccttacgct gaaaagggat tttcaaccca tatttctggc
     7261 cagagcggat atattcaaaa agcctttcct ttcaaaaatt ctcacatttc ttaaaattat
     7321 gcccgtttac cgttcgcgcg acgggtttga aaatctcctt ctcaacgaca ggatattcca
     7381 aaagaccttg gatgtccttc aaaaccgtaa tgggttggtg gtgcttcccg agggaagcca
     7441 tgagggggtt aaaaagctcc gccagctgaa gaaaggaatt gcccgtattg cctttcaggc
     7501 agaggatgcc gccaacggta aacttggtat tcagatagta cctgttgggc tagagtacag
     7561 caactacgta gctttcagaa gcaacctttt aatcaggctg ggtgaaccca ttgaggttga
     7621 ccgctacctt gagctctaca ggcagaatag ggcacaggcc tacaacgctc taattgccga
     7681 gctggagcaa cgcatgaggt tggaaatgat caacatagag gatgaaaact ataccgccta
     7741 ccttaccatc ctggagctat tttcagcaag gtacattaag gagagaaaac tacctaagtc
     7801 acaaaaccag aaatttcagg ttgataagaa aatcatttca ctgctagaca ggctgaaaaa
     7861 tacaaatgct tcggaattcg aggagataat caaaaatgcg aatgattatg gcaggataat
     7921 aagtgc
//
LOCUS       DAOOQX010000010         7883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_11369_length_7883_cov_3.819877, whole genome shotgun sequence.
ACCESSION   DAOOQX010000010 DAOOQX010000000
VERSION     DAOOQX010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11369_length_7883_cov_3.819877"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1824
                     /locus_tag="PLX53_00390"
     CDS             <1..1824
                     /locus_tag="PLX53_00390"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOY71569.1"
                     /translation="YLNLGRAHIALSNFQLALEQYQKALRTFESINNEKGIAVSNSGI
                     GMIYENLSQSVMATEENEVNYTKALEYHKNALRLFRKMGQRKEEGQTLQNIGNVFSRL
                     ATNRFVARFGEEWEDSLYKIKQDQILASFDSSLIYYQYALDIFNQINDEREIVKVNTN
                     LGSTYSWARDWPKATRYISQAVGICRRNGYQYELSAALYAQGESYYRQGNLAGAEVSF
                     LECVEISTRLGLKETLRYSYNRLSKLYEQKGDMAKALANLKLSVRIKDEIFTEKSQKI
                     ITEMQTKYETEKKEQELKLMKNQDELQKSIIQRQKLMIIGAIVGALLILMVALLMFKM
                     FRDKQRANRILQEKNALITSQKKEITDSIQYASRIQTAVLPTSSLLAEALPEHFVLFK
                     PRDIVSGDFYWMTRKNEKVVLVAADCTGHGVPGAFMSMLGVSFLYEIVNKENVLQPAE
                     ILNQLRIHIKTTLSQTGKQNEQKDRMDISLCVIDRPGNVLEWAGAYNSLYQIRGGELT
                     EYKADKMPVAVHVSDSNSFTNHQIEIQHGDTFYMFSDGYADQFGGTDGKKFMSKKFKE
                     LLVGIWNKPMDEQGKIINETHLQWKGEYDQVDDILVIGFRV"
     gene            complement(2346..3659)
                     /locus_tag="PLX53_00395"
     CDS             complement(2346..3659)
                     /locus_tag="PLX53_00395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOY71570.1"
                     /translation="MGNHLKGFRHFTTMEIPEYFQNKNFSPSIQELPTKKLSIVVVIP
                     SFNEADLKDSLMALASCSKPRGDVEVIVVVNHPENSSAEVVTTSEQSLQVVAEADSCW
                     GSSNFRFYAIKAFNLPQKQAGVGLARQIGMDEAAYRLFLAKQPGGIIACFDADATCEV
                     NYLVELENFWAANPDIHACSIRFEHPIQGNNFPQEVYDGIAQYELHLRYYNQALRYTG
                     FPFAFHTVGSSMACTAEAYVKFGGMNRHQAGEDFYFLQKIIPHGHFAELNTTCIYPSP
                     RASNRVPFGTGKSMAMFLENPGNEIQTYQLESFFPLQMLFGKMQSLYQQDVGQIDCFI
                     DSMPAPLKLFLKQNNATEKIYSISRNTASPGSFRKRFFLWFDAFLMLKYLNFVHNRFF
                     KRQPVKHEAIRLAGIIGLRVDERWPVEKILVKYRELEANKFNVNP"
     gene            complement(3706..4326)
                     /locus_tag="PLX53_00400"
     CDS             complement(3706..4326)
                     /locus_tag="PLX53_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOY71571.1"
                     /translation="MKKSTLIFNIILSLAVVALFILHFTQKSPRNHVSGSTPSEASAA
                     LSEGLSVAWVNMDTLLNHYDMYFDMRKELENNSRNLEAQLNSKSKAFEQDAMDFQNKV
                     QKGLVTRSEAQQLQTSLANREQELYKLRDDMQMQLAEEEQVKLRQIHYSITEYLKKYN
                     ADKGYHIILSSNFGGPLLYGHPALDITSEVIGGINQEYAANHKTDK"
     gene            complement(4576..4959)
                     /locus_tag="PLX53_00405"
     CDS             complement(4576..4959)
                     /locus_tag="PLX53_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HOY71572.1"
                     /translation="MSLSFNPNAFDNLIVVGDRVLIKPKNPDERTKAGLLLPPGVQEK
                     QKAHSGYVVKVGPGYPIPTIGELDEPWKKSRENARYFPLQAQVGDLAVYLHENSIEVE
                     FNNEKYVVVSFSSILLLFRDEGLLE"
     gene            5334..6731
                     /gene="asnS"
                     /locus_tag="PLX53_00410"
     CDS             5334..6731
                     /gene="asnS"
                     /locus_tag="PLX53_00410"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOY71573.1"
                     /translation="MRNSERVKVKNLLESGTVGAEVAAMGWVRTKRGNKSITFIALND
                     GSTINSIQVVFDMAVFSEDQLKNVATGACIGVRGKLVESPGSGQRVEIQASYLEVLGE
                     ADSATYPLQKKGHSMEFLREIAHLRPRTNTFGAVLRIRHALAFAIHKYFNDRGFIYLH
                     TPIITGSDAEGAGAMFRVTTLDPNNLPRTPEGKIDWSEDFFGKEAKLTVSGQLEAELG
                     AMALSEVYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYDIQDNMDLAEDFLKYLIRYV
                     LTSCADDLEFLKKMYDDELVSRLQLVVDNKFERLTYTRAVEILEKANQKWEFPVFWGA
                     DLQSEHERYLVEKHFSKPVILTDYPKEIKAFYMKQNDDGKTVRAMDVLFPKIGEIIGG
                     SQREENYQKLLSRITELGVPEKDVWWYLDTRKFGTAPHSGFGLGFERLVLFVTGMSNI
                     RDVIPFPRTPKNCEF"
     gene            6738..>7883
                     /locus_tag="PLX53_00415"
     CDS             6738..>7883
                     /locus_tag="PLX53_00415"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HOY71574.1"
                     /translation="MLKQRLQQKLLQKLSPQQIQMIKLLEVPTMLLEQRIKKEIEENP
                     VLEEVHDAEEPENDTEASLVNDDSDTETDKNDDEFTLEDYLNDEDIPNYRLVASNVSK
                     DDKREEIPFSVGVTFQESLKSQLGLTSLNDREKQLALYLIGNIDEDGYLRRKLSAIAD
                     DVAFALGLDTNEDELEKILYVIQEFDPPGVGARDLQECLLLQLRVKENGSTTASTAYK
                     ILKYHFDEFTRKHYDKIAQKLSLSDEQLRDAINEILKLNPKPGGGFSDPYNQSIQQVI
                     PDFILDYVDGEFQLNLNSRNVPELRISREYSDMLESYSYSKDSATSQEKEAVTFVRQK
                     IESARWFIDALKQRQNTLMTTMRAILNYQQEFFRDGDESKLKPMILKN"
BASE COUNT         2217 a         1735 c         1892 g         2039 t
ORIGIN      
        1 tacttaaacc tgggacgtgc gcatattgcg ttaagcaact ttcagctggc actggagcag
       61 taccaaaagg cactccgaac tttcgaaagc atcaacaacg aaaagggcat tgccgtttcc
      121 aattccggca ttggcatgat ttacgagaat ctctcgcaaa gcgtcatggc cactgaggaa
      181 aacgaagtga actacaccaa ggcgctagag taccataaaa atgcgttgcg cctgtttcgt
      241 aaaatggggc agcgaaagga ggaggggcaa acactccaga acataggcaa tgttttctca
      301 cgacttgcaa ccaaccgttt tgttgcccgg ttcggggagg aatgggagga ttctctctac
      361 aagattaagc aagatcaaat tctcgcctcg ttcgatagct cacttattta ctaccagtac
      421 gcacttgaca ttttcaatca aataaatgat gagcgggaaa ttgtaaaggt aaacaccaac
      481 ttaggaagca cttacagctg ggccagggat tggccaaaag caacccgata catctcccag
      541 gctgttggta tctgccgtcg taatggctac cagtatgaac tttcagctgc gctttatgct
      601 cagggcgaaa gctactaccg ccagggcaat ctggccgggg ctgaggtttc atttctggag
      661 tgcgttgaaa tatctaccag gttaggtctg aaagaaactc ttcgctacag ctacaacagg
      721 ctatcaaaac tttacgaaca aaagggagac atggccaagg cgttggccaa tcttaaactt
      781 tcggttagaa ttaaagacga gattttcacg gagaaaagcc agaaaatcat aaccgaaatg
      841 caaaccaagt acgaaactga aaagaaggag caggagttaa agctgatgaa aaaccaggat
      901 gagctccaaa agtccatcat tcaacgtcag aagttgatga taatcggcgc aattgtcgga
      961 gccctgctga ttcttatggt tgccctcctg atgtttaaga tgttccgcga caagcaaagg
     1021 gccaacagga tacttcagga gaagaatgcg ttgataacca gccagaaaaa ggagattacc
     1081 gatagcattc agtatgcaag ccgcatacaa acagccgttc ttcctacctc ttcacttctg
     1141 gccgaagccc tgcctgagca ttttgtactt ttcaagccaa gagatattgt tagcggcgat
     1201 ttctactgga tgacccggaa aaatgaaaag gttgttctgg ttgctgccga ctgcaccggc
     1261 cacggagtac caggggcttt tatgagtatg cttggggttt catttctcta cgagatagtt
     1321 aacaaggaga atgtgctgca acctgcagaa attctaaacc agcttcgcat ccatatcaaa
     1381 acaacccttt cacaaacagg gaagcagaac gagcaaaaag atcgaatgga catttccctt
     1441 tgtgtaatcg accgcccggg caatgttttg gagtgggcag gtgcctataa ttccctctac
     1501 caaattcggg gcggagagct aaccgagtac aaagccgaca agatgcccgt ggctgttcac
     1561 gtaagcgata gcaactcctt taccaaccat caaatcgaaa ttcagcatgg cgataccttc
     1621 tacatgtttt ccgatggcta tgctgaccag tttggcggaa cagatggtaa aaagtttatg
     1681 tcgaaaaaat tcaaggagct gctggtagga atttggaata agcccatgga tgaacaaggc
     1741 aaaattatta acgaaaccca ccttcagtgg aagggcgaat acgatcaggt tgacgacatc
     1801 ctggtcatag ggttcagggt ttgaaggtaa ccggtagagg gcatttaagg ataaagggta
     1861 aagggtaaaa taattcaaga acttgtccgc ctactggcgg atttcagaac ctgtccgcct
     1921 accggcggat tccagattca aaattcaaga tttcagaact tgtccgccta ccggcggatt
     1981 caagaacttg tccgcctccc ggcgcattca cccttcggac aggctagggg ttctaaaact
     2041 atcccgtaca tgatggattc agcattcacg tcgtgttaaa aaatagtttt agtcccgaag
     2101 aggcgtaaca tggatagccc cgggcgtaag ccagagttga tgagggtcgg cggtggttgg
     2161 cgatgcaagt agtaagtttt ccaaagcgca acccccacta actgcatcgc aacgatagga
     2221 attttccaaa gcacagggac aaggcaaggg taaaggataa agtttaaagg ataaaaagta
     2281 atggctaaag ggtaaagaaa ttaaaggttc acggttcact tctcacggct ttttaccttt
     2341 tacccctatg ggtttacgtt gaatttattg gcctccaact cgcggtattt aaccagaatt
     2401 ttttcaactg gccaacgttc atctacccga aggccaatta taccggcaag cctgatagcc
     2461 tcgtgcttca ccggttgccg tttgaagaaa cggttgtgaa cgaagttcag gtacttcaac
     2521 atcaaaaagg catcaaacca gaggaaaaat ctttttctga acgaaccggg agatgccgta
     2581 ttacggctaa tgctataaat cttctcggtt gcattgtttt gcttcaggaa tagttttaat
     2641 ggggcaggca ttgaatctat gaagcagtct atttggccaa catcttgctg gtagagcgat
     2701 tgcattttac caaagagcat ctgtagcggg aagaacgatt caagctggta ggtttggatt
     2761 tcattaccgg gattttccag gaacatggcc attgatttac ccgttccaaa gggcaccctg
     2821 ttcgacgccc ggggtgaagg gtaaatgcaa gtagtgttta actcggcaaa atgcccatgg
     2881 ggaattattt tctgcaggaa gtagaagtcc tctcctgcct ggtggcggtt catgccgcca
     2941 aacttaacgt aagcttctgc ggtgcaggcc atggatgagc ctactgtgtg gaaggcaaaa
     3001 gggaatccgg tgtagcgcaa tgcctggttg tagtagcgca aatgcagctc gtattgagca
     3061 atgccatcat acacctcctg tggaaaatta ttaccctgaa tgggatgctc aaaacggata
     3121 gagcaggcat gtatgtcagg gttggctgcc caaaagtttt ccagctcaac caggtagttt
     3181 acctcgcagg tggcatcggc atcgaaacag gcaataatgc ctcctggttg tttagctagg
     3241 aacaagcggt atgcggcttc atccatacct atctgtcgtg ctaaccccac tccggcctgc
     3301 ttttggggga ggttaaaggc tttaatggca taaaacctga agttgctgct gccccaacaa
     3361 ctatctgcct cagccacaac ctgaagcgat tgttctgagg ttgttaccac ctcggccgaa
     3421 gaattttcgg gatggttaac caccacaata acctcaacat ctccacgtgg tttgctgcag
     3481 ctcgcaagtg ccattaacga atccttcaga tctgcctcat tgaaggaagg tattacaacc
     3541 actatggata acttttttgt tggcagctcc tgaatggatg gggaaaagtt cttgttttga
     3601 aagtattcgg ggatttccat tgttgtaaaa tggcgaaacc ccttcaaatg gttgcccatc
     3661 gaaagggttt cggttggttt aggtttagtt taggtaggtt ataaattact tgtcagtttt
     3721 atggttggca gcatattcct ggttaatgcc gcctatcacc tcgctggtta tgtccagtgc
     3781 cggatgaccg taaagcaacg gaccgccgaa gttactgcta agaataatgt ggtaaccctt
     3841 atcggcatta tacttcttga ggtattcggt aatactgtag tgtatctggc gtagcttaac
     3901 ctgctcttct tcggcaagct gcatctgcat gtcgtcgcgc agcttgtaaa gttcctgctc
     3961 ccggttggcc agggaggttt gcagctgctg ggcttcggag cgtgtaacca gccccttttg
     4021 aaccttattc tggaaatcca tggcatcctg ctcaaaggct tttgattttg agttcagctg
     4081 tgcctccagg tttcggctgt tgttctccag ctccttccgc atgtcgaagt acatatcgta
     4141 gtggttgaga agggtatcca tgttgaccca ggcaactgat agtccttcag aaagggctgc
     4201 acttgcttct gatggggtag agccggaaac atggtttcgt ggcgattttt gagtaaaatg
     4261 aaggataaag agggcaacca cggcaaggga gagaataatg ttgaaaatta aggttgattt
     4321 tttcatcttc aaaacgttta gctgttagtt atacagattg gtttaattcg gtttaaatgt
     4381 attacatcct gaaacgcttc ataaaactat tcaacaccag ctactattag ttcaattagt
     4441 cctgttttaa aaggaaaaac tacaggttaa actttaaacc ccaataaata ttggcatttc
     4501 aagtatctca cttgcaaata taagccatta atcgaaaaaa tgtatttttt tgataaaaaa
     4561 atcgaatggc tgcatctact ccaaaagccc ttcatcacga aagagaagta gaatggagga
     4621 aaacgaaaca acaacgtact tctcgttgtt aaattccacc tcgatggagt tttcgtgaag
     4681 gtaaacggca aggtcgccca cctgcgcctg aagcgggaag tatcgtgcat tttccctcga
     4741 ttttttccat ggctcatcaa gctcacctat tgtgggaatg gggtagccag ggccaacctt
     4801 tacaacgtaa ccgctatgag ctttttgctt ctcctgaact cctggtggca acagcagccc
     4861 ggccttagtc cgttcatcgg gatttttcgg ctttattaat actcgatcgc caaccacaat
     4921 taggttgtca aacgcgttgg gattgaatga aagcgacatc gggttgggta tatggattta
     4981 taaatacttg tttttcggtc attcgaggct aatcctggtt agctggcatt tctgcaaata
     5041 taaaacttgt ttagctaatg cgatattttt aactttttag ccagccgttg cattatttta
     5101 aaagtggctg tttgcttact gatgtcggca tagcacaacc aacctgtgcg agaccagccg
     5161 gctttggagg tttatatttg catttggttg ctgttccctc tatttttaga cattttgatt
     5221 gaaagaattc aaccttgaca ttcttaatcg tcgaaattcg agcttagata gccaactatt
     5281 ttatagtttt gtcgtgattt tgcagttcga ttaaaaattg aattttcttg attatgagga
     5341 atagcgaaag ggttaaggta aaaaatttgc tcgagagcgg aacagtagga gctgaggtag
     5401 ctgcaatggg atgggttcgc accaagcggg gtaacaagag tatcaccttt attgcgctta
     5461 acgatggctc cactatcaac agcatacagg ttgtttttga catggctgtg tttagcgagg
     5521 atcagctaaa aaatgtagct actggggctt gtatcggtgt taggggaaag ctcgtagaat
     5581 cgcccggttc agggcaacgg gttgaaattc aggccagcta cctggaggtg ctgggcgaag
     5641 ctgattcggc aacatatccc cttcagaaaa agggtcatag catggagttt cttagggaaa
     5701 ttgcccacct gcgtccccgt acgaatactt ttggcgccgt gcttcgcatt cggcatgctt
     5761 tagcttttgc aattcacaag tacttcaacg ataggggatt catctacctg catactccta
     5821 ttattaccgg ttctgatgcc gagggtgcag gggccatgtt ccgcgtaacc acgctcgacc
     5881 ccaataacct tccacgaact ccggagggaa agattgattg gagtgaggat ttctttggta
     5941 aagaggcaaa gcttaccgtt tcgggacaac tcgaggcgga actgggtgca atggctcttt
     6001 cggaggttta cacctttggc cctacctttc gagccgaaaa ttcaaacacc ccccggcatc
     6061 tggccgagtt ctggatgatt gagcccgaaa tggcctttta tgatattcag gacaacatgg
     6121 atctggccga agatttccta aaatacctta ttcgctacgt gctgactagc tgtgccgatg
     6181 acctggaatt cctaaaaaag atgtatgacg atgagctggt tagcaggtta cagcttgttg
     6241 ttgacaacaa attcgagagg ctaacctaca cccgcgccgt cgaaatcctt gagaaggcaa
     6301 accaaaaatg ggaattcccc gtattctggg gtgcagacct acagtcggaa catgagcgct
     6361 acctggtgga gaaacacttt agtaaacccg tcattctaac cgactatccc aaggagatca
     6421 aggcattcta tatgaaacaa aacgatgacg ggaaaaccgt cagggcaatg gatgtgcttt
     6481 ttccaaaaat tggcgaaata attggtggtt cacagcgcga ggagaactac caaaagctcc
     6541 tttcacgcat taccgaactt ggcgttcccg aaaaggatgt ttggtggtac cttgataccc
     6601 gtaagtttgg aacagcaccc catagcggat ttggacttgg ttttgaaagg ctggtgctct
     6661 ttgttaccgg catgagcaac atcagggatg ttattccatt tcctagaacc cccaaaaact
     6721 gtgaatttta gagtaaaatg ctaaagcagc gattacaaca aaaacttctt cagaagttat
     6781 ctccacagca aatacagatg attaagctgc tggaggtccc taccatgctg cttgaacagc
     6841 gaattaaaaa ggaaattgaa gaaaatccgg tattggagga ggttcatgat gctgaagaac
     6901 ctgaaaacga taccgaagca tcgctcgtaa acgacgattc cgataccgaa accgacaaga
     6961 acgacgatga gttcaccctt gaagattacc ttaacgacga ggacatcccc aactaccggc
     7021 tagttgccag caatgtgtcg aaggatgata aaagggagga aatcccgttc tcggtggggg
     7081 taacatttca ggagagccta aaaagccagc tggggttaac aagccttaac gaccgtgaga
     7141 agcagctggc tctttacctg attggcaaca tcgacgagga cggctacctg cgcaggaaat
     7201 tgtcggccat tgccgatgat gttgcctttg ccctaggcct cgataccaac gaggacgagc
     7261 tggagaaaat tctttacgtt attcaggagt ttgacccacc gggagttgga gcccgtgacc
     7321 tccaggagtg cctgctgctc cagctcaggg taaaggaaaa tggaagcacc actgcatcta
     7381 ctgcatataa aattttaaag taccattttg acgaatttac ccgaaagcat tatgataaaa
     7441 ttgcgcagaa gctttcactc tccgacgaac agcttcgcga tgccatcaac gaaatactta
     7501 agctaaaccc aaaacccgga ggaggattta gcgatcctta caaccagtca attcagcagg
     7561 ttatccccga ttttatcctc gactatgttg acggggaatt tcagcttaac ctcaactccc
     7621 gaaatgttcc cgagttaaga atcagccgcg agtattccga catgcttgaa tcgtatagct
     7681 atagcaagga tagcgcaacc agccaggaga aggaggctgt gacttttgta aggcaaaaaa
     7741 tcgaatcggc acgctggttt atagatgccc ttaagcagcg gcaaaacacc ctgatgacca
     7801 cgatgagggc aatacttaac taccagcagg aattttttcg tgatggggac gaatcgaagc
     7861 tgaaacccat gattctgaaa aat
//
LOCUS       DAOOQX010000011         7743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_11638_length_7743_cov_3.842612, whole genome shotgun sequence.
ACCESSION   DAOOQX010000011 DAOOQX010000000
VERSION     DAOOQX010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11638_length_7743_cov_3.842612"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLX53_00420"
     CDS             complement(<1..588)
                     /locus_tag="PLX53_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71575.1"
                     /translation="MKPIIQATSISTVTYISNLLIGLTIALTQFFTAEAYSQDTLKTY
                     CNPINIDYGYSPIPAAIEQVKHRATADPLVVAFKGKFFLFSTNQHGYWWSSNMASWNF
                     IPRKFLLPRHKVYDDLCAPAAFVLGDSLLLIGSSNNPDFPIWASTNPAADDWHIAIDS
                     FPLAAWDPAFFADDDGRVYLYFGSSNLYPIYGCEVN"
     gene            864..2069
                     /locus_tag="PLX53_00425"
     CDS             864..2069
                     /locus_tag="PLX53_00425"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HOY71576.1"
                     /translation="MLSIREVYKEGYGPSSSHTIGPVRAAELFIKKHPEVSYFRVMLF
                     GSLALTGRGHQTDSALRKCFLPKSVEVVFKPEETLPLHPNGMVFEALTDEGAEALDRW
                     EVYSIGGGDLKDKSGVLNSSEVYSLNNMTDILTWCRREGKTFWEFVEDNEGKDIWEFL
                     QGVWHTMRETIKRGIENEGVLPGPIKLARRASSQYSKALTSHGTLKSFGLLFSFALAV
                     AEENAAGGRIVTAPTCGSSGVLPAILFYLSREEYLTEVKIHRALATAGLIGNLVKHNA
                     SISGAEVGCQGEIGTACAMAAAAMVQVLGGSPNQIEYAAEMGMEHNLGLTCDPVLGYV
                     QIPCIERNAIAASKAYSCGVYALSSDGGHKISFDRAVETMAETGRDIQVAYRETGIGG
                     LAKNWEPFE"
     gene            complement(2184..2906)
                     /locus_tag="PLX53_00430"
     CDS             complement(2184..2906)
                     /locus_tag="PLX53_00430"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOY71577.1"
                     /translation="MRRFMITGTFALMTSMGSYAYAQGQVTIQNDSKDFVGVKNSISA
                     TVNLYQASTYKVEITTSQKVADKLEISVENGLLSIREKDQSWNHWSCSNNTDDEVVVN
                     VWSPKYSTLEINGSGDMLAKTPIKTNEIEVKVNGSGDVKINELEAEKVSLKTNGSGDI
                     AVSGKKEASTLVIKINGSGDIDTRNLVTRVADVAITGSGDVSANVLEKFTANSVGSGD
                     IYLSGNPQVDAKILGSGEIVRK"
     gene            complement(3133..3420)
                     /locus_tag="PLX53_00435"
     CDS             complement(3133..3420)
                     /locus_tag="PLX53_00435"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71578.1"
                     /translation="MLEKKLYANGQPVYKLADGMLIFYYKNGTVKAEGPYPDNMMEGE
                     WRFYRESGQLWQIGNFKNNQKHGSWIRYDRNNNVEYQETFEHNKAVNPKRR"
     gene            complement(3612..4052)
                     /locus_tag="PLX53_00440"
     CDS             complement(3612..4052)
                     /locus_tag="PLX53_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOY71579.1"
                     /translation="MTLNEVQDAIVEDFSISNDWMDRYQQLIELGKELPPIDEKKRTD
                     QYLINGCQSKVWLDAELRDGRIFFSADSDAIITKGIVSLLIKVLSGRTPEEIINADLY
                     FIDKIGLRENLSPTRSNGLVAMVKQMRMYALAYQTKLNQQKDNH"
     gene            complement(4105..4494)
                     /locus_tag="PLX53_00445"
     CDS             complement(4105..4494)
                     /locus_tag="PLX53_00445"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOY71580.1"
                     /translation="MLKLELKETATTPYINFDPTSGVIRMEGRSIPENVIDFYQPILG
                     WVEEYGKNIQPETTVHLKFEYFNTSSSKRIFDLMKKFEKIGIETGTKVTINWYYEEDD
                     EDIFFAGNDYKALINKVEFNLIEIKPS"
     gene            complement(4534..5046)
                     /locus_tag="PLX53_00450"
     CDS             complement(4534..5046)
                     /locus_tag="PLX53_00450"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOY71581.1"
                     /translation="MGILVEKSADCPYINFSEDGILEIEGRSITEDPFTFWQPLLEWV
                     ETYTKHPSPKTEVIIYMEYSNSSSNKYINELLRKLEECHRKTSSVRVTWRYEQDDESI
                     YQLGRDFESMLHVPFEFIEVNTSADSSKRIRVKNKSNGSEAIITHRYWEAIVRNGHGE
                     DYQILQEFSN"
     gene            complement(5215..6693)
                     /locus_tag="PLX53_00455"
     CDS             complement(5215..6693)
                     /locus_tag="PLX53_00455"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOY71582.1"
                     /translation="MKKIPEVSNRFFSTNLFLLLLLNLIVKPFWIFGIDRSAQNALGA
                     SDYGLYYALFNFTLIFNILLDCGVTNFNNREISFHPQLAGKYFANILSIKLMLSVVYV
                     AITVAVALGMGYGHHQLGLLFILAVNQFLSSLVMFIRSNLNALHHFGLDSVLSVLDKL
                     IMILLGSVLLWTPLRKNFTIEAFAVAQTLSYMATVAVSLIFLTQTTGRLRIKFDRVLS
                     LSMLKNSLPYALMVLLMTAYSRLDSVVIDRLLPNGSEAAGIYAQAFRLLDAANMLPFL
                     FASLLLPIFSGMIKQKREFGPFLNFSFLLLVVPVVAIAIPTIAHRAPIINLLYHEHAD
                     LSAPVLGLLMISYIFISVGYVFGTLLTANGNIKELNIVSGAAVVVCLSVQITLVPRFG
                     VTGAAIGNMVVNFLVAVAQVTISLKKFRLTPDRTMVFRFLAYLLFCPLATVSILNLHL
                     GMAFSYVLSIAVSLGFALVLKLIDIKQLLATFTTENTPNVKG"
     gene            complement(6992..>7743)
                     /locus_tag="PLX53_00460"
     CDS             complement(6992..>7743)
                     /locus_tag="PLX53_00460"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOY71583.1"
                     /translation="IENRLVLDDLVNTPRLKQSNYQIVHYDSPDRRGVDVGLLYRPEF
                     FKVTSHRSVRLNIPDNPEFRTRDQLVVSGLLDGETIHIIVNHWPSRSGGEKRSQPYRI
                     AAAKLSRSIIDSIRATDADAKIILMGDLNDDPDSRSIIDYLGAKNNAEGLRSGDLFNV
                     MASLYREGIGTLAYQDSWNLFDNIIITQPLLSKDRTSWVFYKAKVFNKPFLRQTSGQY
                     AGYPFRTYVGANYSGGYSDHFPVYIFLIKEK"
BASE COUNT         2038 a         1650 c         1860 g         2195 t
ORIGIN      
        1 gttaacctca cagccgtaaa ttgggtaaag gttgctggag ccaaaataaa gatatactct
       61 gccatcgtca tctgcaaaga aggcgggatc ccatgctgct agtgggaatg agtcaattgc
      121 aatatgccaa tcgtcggcgg cggggttggt ggatgcccaa atgggaaaat cggggttatt
      181 cgaagagccg attagcaaaa gggaatcgcc cagcacaaag gccgcaggag cacagagatc
      241 gtcgtaaacc ttgtgtcggg ggagtaaaaa ttttctggga ataaagttcc agctggccat
      301 gttgctactc caccagtagc catgctgatt ggtagaaaat aaaaagaatt ttcccttgaa
      361 tgccaccacc agcgggtctg ctgtagccct atgttttacc tgctcaattg ctgctggtat
      421 tggactatag ccgtaatcaa tattaattgg gttgcagtag gttttaaggg tatcctgcga
      481 ataggcttca gctgtgaaga attgcgtgag cgctatagtt aaccctatca gtaaattcga
      541 tatgtatgtt acggttgata ttgaggtcgc ctggataatg ggtttcatga ttcttgcaat
      601 ttatggatga aggacaacat gccgtggtgc aatagcaata agtatgtatc gggcaaacag
      661 gctcgttatg ttttggatca agtgccgaaa aacctactgc gcaacttggc atgtttagat
      721 tctcaaaatt aaaaatttat tacgacatgc ccgcagagag gcaacattta tacttttcag
      781 taaaaattgt gggggcatta ctttcagccg tttgcccctt taaaaaattt gatacagctt
      841 tgcagcctaa atttttaaga caaatgctat caattcgtga ggtttataag gaggggtatg
      901 gcccgtcgag tagccatacc attgggccgg taagagctgc agagctattc ataaagaagc
      961 atcctgaggt gagctacttc cgggtaatgc tatttgggag tttagcgcta acgggtaggg
     1021 gtcaccaaac cgatagcgct ttacgaaagt gttttcttcc aaaatcagtg gaggtggtgt
     1081 ttaagcctga ggaaacgcta ccgcttcatc ctaacgggat ggtatttgag gcgttgaccg
     1141 acgagggtgc cgaggcgctt gaccggtggg aggtttacag cattggcggc ggtgatttaa
     1201 aggataaaag cggggtgttg aatagcagtg aggtttattc gctcaacaac atgaccgata
     1261 ttttgacctg gtgtaggagg gaagggaaaa ctttttggga gtttgtggag gataacgagg
     1321 gaaaggatat atgggaattt ctgcagggag tttggcatac catgagggaa acgataaaac
     1381 ggggtattga aaacgagggg gttttgcctg gtccgataaa gcttgccagg agggcttcat
     1441 cgcagtattc gaaagcactt accagtcatg gaactttaaa aagctttggc ctactgtttt
     1501 cttttgccct ggcagttgcc gaggaaaacg ctgcaggtgg aagaattgta actgcgccaa
     1561 cctgtggctc gtcgggagtt ttaccagcca tactgttcta cctgagccgg gaagagtacc
     1621 ttaccgaggt aaaaattcat agggcgctgg ctactgcggg attaattggc aacctggtta
     1681 agcacaatgc ttcaatttca ggagcagaag tgggctgcca gggagaaatt ggaactgctt
     1741 gcgcaatggc tgccgctgct atggtacagg ttcttggagg atctccaaac cagattgagt
     1801 atgccgccga gatgggtatg gagcataacc ttggcctaac ctgcgatccg gtgttgggct
     1861 acgtgcaaat accctgcata gagcgtaacg ccattgctgc atccaaggca tactcctgcg
     1921 gagtgtacgc cctttcctct gatggagggc ataaaatttc gtttgaccgg gcagttgaaa
     1981 ccatggccga aaccggccgt gatattcagg tggcttacag ggaaaccgga attggcggac
     2041 tggcaaaaaa ctgggaacca ttcgaatagg caatggtgca gttttttttc aaaaatggaa
     2101 aggcatcgga attgctccct ttgccctgcc atttcagcaa aacctctatt ttcacctaaa
     2161 cctattcgtg ttgttgcttg atgctactta cggactatct cacccgaacc taatatcttt
     2221 gcatcaactt gtgggtttcc tgagaggtaa atatcgccgc taccaacaga gttggcggta
     2281 aatttttcca gtacgttggc cgaaacatca cccgaaccgg taatggctac gtcggctacc
     2341 ctggtaacca ggttgcgggt atcaatatcg cccgaaccgt tgattttgat taccagcgtt
     2401 gatgcctcct tttttccact tacagcaata tcgcccgagc cgtttgtctt gagggaaact
     2461 ttttcggctt ccagctcatt tatttttaca tcgccgctac cgttaacctt tacctcaatt
     2521 tcgtttgttt tgatgggggt ttttgcaagc atatcgcccg aaccgtttat ttcaagcgtt
     2581 gaatatttag gagaccaaac gtttaccacc acctcgtcat ctgtgttgtt gctgcaactc
     2641 caatgattcc atgattggtc cttctccctg atgctcagca gaccgttttc taccgaaatc
     2701 tccagcttgt cggctacctt ctggctggtt gttatctcaa ccttgtatgt ggaagcctgg
     2761 tagaggttta ctgtagcgga gatgctattc tttaccccta caaaatcctt gctgtcgttt
     2821 tggattgtaa cttgtccctg cgcgtacgca tagctgccca ttgaagtcat cagggcaaat
     2881 gtgccggtaa tcataaatct tctcatggtt tactcttttt agaaagattt ttacttcaaa
     2941 tcgatgtatc cctgtaagta gcaggcttag cctttggctt aaaaagaaaa tcatttttgc
     3001 tccgtggaaa atacatttat cttttcaata gacttctatc cgatgcggaa tgctgcatgg
     3061 agggtgattt ttttataaac ctatatactg aggcagttag ccatatcttt catgtaaaac
     3121 atgagaatag tgttaccggc gtttagggtt tacggccttg ttgtgttcaa aagtctcttg
     3181 gtactccacg ttgttattcc tgtcataccg tatccaggaa ccatgtttct ggttgttttt
     3241 gaaatttccg atttgccaaa gttgacccga ctccctgtaa aaacgccatt ctccctccat
     3301 catgttgtca ggatatggcc cttcagcctt tactgtcccg tttttgtagt aaaaaattag
     3361 cattccatcg gccaacttgt aaacaggctg tccgttagcg taaagttttt tttcgagcat
     3421 tggtgtaaac ttttttattg atgatggatt caagttataa atggaacttt taaaaagaga
     3481 aagatgagta tagcgtaaac aacaggctat cggattaatc gccagaactt ttacccacca
     3541 gcatcagctg agttcaagtt catcgtgcta agtcaacctg tggtgggaga attaataggc
     3601 ttccccagtt gttaatggtt atccttttgc tggttaagtt tggtttggta ggcgagtgcg
     3661 tacatgcgca tctgcttaac catggccaca aggccgttgc tgcgggtagg tgagaggttc
     3721 tcccttaatc caattttgtc aatgaagtat aggtcggcgt tgatgatttc ctcgggagtt
     3781 ctacccgaga gtaccttgat gagcagcgac actatcccct tggtgattat ggcatcgctg
     3841 tcggctgaga agaaaatcct tccgtcacgc agctcggcat ccagccaaac cttactctga
     3901 cacccgttta tcaggtactg gtcggtgcgt ttcttttcgt caattggggg cagctcctta
     3961 cccagctcta tgagctgctg gtagcgatcc atccaatcgt tggaaatgct gaaatcctca
     4021 actatggcat cctgaacctc attaagggtc attgtatgtg gattattttg ttgtatgcgt
     4081 gcttgtaaac agaacgaaat gcttttagct gggtttaatc tcaatcaggt tgaactccac
     4141 cttgttgatg agcgccttgt agtcgttgcc tgcaaaaaat atgtcttcgt catcctcctc
     4201 atagtaccag ttgatggtaa ctttcgtacc ggtttcaatg ccaatctttt cgaacttttt
     4261 catcaggtca aaaattcgtt tcgaggagct ggtgttgaag tactcgaatt tcaaatggac
     4321 ggtggtttcg ggttgaatat ttttaccgta ttcctccacc catccaagaa tgggctggta
     4381 gaaatcaata acattttcgg gaatcgacct gccctccatc cttataaccc cgcttgtagg
     4441 gtcaaagttg atgtaggggg ttgttgcggt ttccttgagc tcgagtttta gcatctacaa
     4501 aatttttatt ggggcaaata tgcaaaaatt atgctagttg gaaaattcct gaagtatctg
     4561 gtagtcctcg ccatgcccat tccgtacaat agcttcccag tatctatggg taattatagc
     4621 ttcgctaccg ttgcttttgt tcttaaccct aatccgtttt gagctatcgg cgctggtgtt
     4681 cacctctata aactcaaacg ggacgtgaag catggattca aaatcccttc cgagctggta
     4741 aatcgactcg tcgtcctgtt cgtaccgcca ggtaacccgt acgctggatg ttttgcgatg
     4801 gcactcctcc agctttcgta gtagctcgtt gatgtactta ttggagctac tgttggagta
     4861 ctccatgtaa ataatcacct cagtttttgg agacggatgc ttcgtgtagg tttcaaccca
     4921 ttctaaaaga ggttgccaaa aggtaaaggg gtcttcggta atggaccttc cttctatttc
     4981 cagaatccca tcctccgaaa aattgatata cggacaatcg gcactttttt caactagtat
     5041 gcccatcgtt atttacctga atttcttaaa gcccataaag atataagttt gtttttaaat
     5101 gtgcaaaatt tctttaagga agctgtccat cactactaat gttttgtggc agcgtgtatc
     5161 cttatgagtt ttcgtatgct tcaataatga accggtaaat gaaaaatgat gagttcaccc
     5221 cttcacgttg ggagtatttt cagtggtgaa ggtggcgaga agttgtttta tatcaattag
     5281 ctttagcact aaggcaaaac caagagatac cgcaatggac aaaacatacg agaaagccat
     5341 gcccagatgc agatttaaaa tggaaacagt ggccagtgga caaaatagaa ggtaggctaa
     5401 aaagcgaaaa accatcgttc tgtcaggagt gaggcgaaac tttttgaggg aaatagtaac
     5461 ctgtgcaaca gctaccagga agtttacgac catgttgcca attgccgcac cagttacccc
     5521 aaatctgggc acgagcgtta tctggacaga aaggcaaaca acaacggcag ctcctgaaac
     5581 gatgttgagc tccttgatat tcccattcgc ggtaagcagt gtaccgaaca cgtaacccac
     5641 tgaaatgaag atgtaagaaa tcataagcag ccccagaacc ggagcggaca aatcagcatg
     5701 ttcgtggtag agaaggttta taattggcgc cctgtgggca attgttggaa ttgcaatggc
     5761 tactaccggc accaccagta gcaggaagga aaagttaagg aaagggccga actccctttt
     5821 ttgcttgatc atgcctgaga aaattgggag taaaagtgaa gcaaaaagaa atggtagcat
     5881 attggcggca tcgagcagcc tgaatgcctg agcgtatatt cctgccgctt cgctgccgtt
     5941 gggcaacagc cggtctatta ccactgagtc taaacggctg taggccgtca tcagcaaaac
     6001 catcagggcg tatggcaagc tattttttaa catgctaagg gataaaaccc tgtcgaattt
     6061 aatccgaagc ctgcccgttg tttgtgtaag aaatattagc gataccgcaa cggttgccat
     6121 gtacgacaag gtttgagcta cagcaaatgc ttctatggtg aaattctttc tgaggggggt
     6181 ccaaagaagc accgagccca gcagaatcat gatgagctta tccagcaccg acagcacgct
     6241 atctaggcca aaatgatgaa gggcgttgag gtttgacctt ataaacatta ccagggacga
     6301 taaaaactgg ttgactgcta gtatgaatag caggccaagt tgatgatgcc catatcccat
     6361 acccagtgca acagccactg ttatcgccac atacaccaca gacagcatta gcttgatgga
     6421 aaggatgttg gcgaagtact taccggcaag ttgcggatga aagcttattt ccctgttgtt
     6481 gaagttggtt accccgcaat cgagtagaat gttgaaaatt agggtgaagt tgaacagggc
     6541 atagtacaac ccgtaatcac ttgcccccaa ggcattttga gccgacctgt ctatgccaaa
     6601 aatccagaac ggcttaacaa tcaggttaag cagaagtagt aaaaacaggt tggttgaaaa
     6661 gaagcggttg gaaacttcgg gtattttttt cacatccggc tacgctttaa atgataccta
     6721 aacgtgaatg ctttaaagaa tattttgcca aaatgaataa ataggataga gcccggcaat
     6781 gttgatgtgg ccgaggccta tctggctata attcagctac cattatgctt gcgttcactt
     6841 cataccgaaa ctattattgc caacatatgg ctaaaagata gggtatgtag catacttggt
     6901 gctggcagca ggtttcatct gggagatttt cctgcacttg acctgctgat gcgtggttat
     6961 acctggcagg ggtgctgatt aaacaagttt gctacttttc cttgataagg aatatgtaaa
     7021 cgggaaaatg gtcggagtag ccacccgagt aatttgcacc tacataggtt ctgaaagggt
     7081 acccagcata ttgcccgcta gtttgcctta aaaatggttt gttgaacacc tttgccttat
     7141 aaaataccca gctggttctg tccttgctaa gcaagggctg tgtgattatg atattatcaa
     7201 aaagattcca agagtcctgg taggcaagag ttccaattcc ctcacggtaa agactggcca
     7261 taacgttgaa aaggtctcct gacctcagcc cttcagcgtt gttttttgcc ccaaggtagt
     7321 ctatgatact cctgctgtcg ggatcgtcgt tcaggtcgcc catcaggata attttggcat
     7381 cagcatcggt tgctcgaatt gagtcaatga tggagcggga gagcttagca gctgcaattc
     7441 ggtatggctg gctacgtttt tccccaccac ttctcgatgg ccagtggtta acaataatat
     7501 gaatggtttc gccatcaagc aagccgctaa caaccagctg atccctggtt ctgaattcag
     7561 ggttgtctgg aatgttcagc ctgaccgatc ggtgggaggt aaccttgaaa aactcggggc
     7621 ggtatagcaa acccacatca acgcctcgtc tgtcgggcga gtcgtagtga acaatttggt
     7681 agttcgattg ttttaaccta ggagtgttta ccaggtcgtc cagcaccaac cggttttcaa
     7741 ttt
//
LOCUS       DAOOQX010000012         7422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_12289_length_7422_cov_3.078594, whole genome shotgun sequence.
ACCESSION   DAOOQX010000012 DAOOQX010000000
VERSION     DAOOQX010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7422
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12289_length_7422_cov_3.078594"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..628)
                     /locus_tag="PLX53_00465"
     CDS             complement(182..628)
                     /locus_tag="PLX53_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOY71584.1"
                     /translation="MRDDFNEDLLNLPFNIHRTSVAIRGEITRRLSEAFGDDFTVDYW
                     YILTLLFSREPLSQAHLAQISGRDKGSLSRTLACMERLDLVVRLPNPDNSRSELVSST
                     KAASNFKPKAESIILDVLSEALSSLRPIEVIELNRMLAAINEKIQP"
     gene            1007..3421
                     /locus_tag="PLX53_00470"
     CDS             1007..3421
                     /locus_tag="PLX53_00470"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOY71585.1"
                     /translation="MEYRLYFSIPNRLSSPVPGRAELNFELINRKDLVIDFMTEATNL
                     QSITVNNRLLSIRVKNGHINIPSKYLLTGQNKVNIDFVSCSPSLNRKADYLYTLLVPD
                     RASTVFPCFDQPDLKAAFNLTLEIPKEWVAVANAPLGKIIESDTSTVYVFEKTKPVST
                     YLFAFACGKFKVASQPTRYGEMAIYYRDNDSAKVNRNIEAIYELHTQSLGWMENYTAI
                     PYPFSKLDIVLIPDFQYNGMEHPGAIYYRESSILLEGDPSENKLLNRANLIAHEVSHQ
                     WFGDLVTMRWFNDVWLKEVFAGLMADKIVNPQFPEFNHQLSFLLSHYPRAYSVDRTAG
                     ANPIRQELDNLLNAGSLYGDIIYHKAPIALMQLEMAMGVSAFQEGLRKYLNTYSMGNA
                     SWDELIDILDPLTSLNLSVWSKAWIESVGMPTLKATLNDSMYVVKKIGVQLPMVYSVE
                     VLCGSNVNEAKPIQHIQPEVSTLFASSCELERVIVPNADGLGYGMFVPDSQSLKLLCD
                     SSFRIADPVTRSASLISANELFLNGKLDGNTYFNMLLASLARENEPQVRSYILASIET
                     YWWTFLADSARENLSQQVEIQFSKLLLNSSIPADERKSIFKTYARIAVSSNAAEELYG
                     IWSGNKSLPGLTFTETDLTFLAYELAVRNVHNADSILDLQEKRIANPDLLAKFRFVRR
                     ALSPDDALRDDFFHSLLIPENRRPEPWVAEALHYFNHPLRDSFASQYLRASLDLLPEI
                     QQTGDIFFPKTWLDAILWGKNSNQAAEIVSSWLNQHPNVPDNLKNKLIQSSDLLFRAV
                     DKRKKK"
     gene            complement(3504..5195)
                     /locus_tag="PLX53_00475"
     CDS             complement(3504..5195)
                     /locus_tag="PLX53_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HOY71586.1"
                     /translation="MNKIKPIMPVMLMIVLVAASSCTSNQKKMEKQMADFIAKVESKV
                     IPLSKEAAIASWDASISGKDEDYQRSEKAQVELVKIFADTSLFAELKSIKESGLVKDS
                     VLSRQMDILYLAFLRNQVDPTLLEKEIAMEANLEKKYSNFRAKIGSKQLTDNEIEDIL
                     ATSTQSSEVESAWKAHKKIGPEVAPEIIELVKVRNQIAQKLGYANYHTMNMVLSEQDP
                     DEVLALFDELDSLTSGSFSELKLEIDSYLSKRFGVSPDKLMPWHYQNRYFQEAPKIYN
                     VNLDQFYSDKDVVALTAKFYNGIGLNINDIIARSDLYEKPGKNQHAYCTDIDRSGDVR
                     VLCNVKPTYGWMNTLLHENGHAVYSKYNDPAKPYFLRDAAHSFTTEAIAEMMGRLASN
                     PQWMLDMGIITPEQKEQIASECLKSLRLEQLVFSRWAQVMYRFEKEMYANPDQDLNSL
                     WWSMVEKYQLLKKPEGRNEPDWASKIHVALYPCYYHNYLMGEMLASQLNAYIAANVLK
                     ISDFNAASFVGHPEVGAYLKEKVFVPGCLYPWNEMIEKATGEKLTAKYYALQFVK"
     gene            complement(5428..6309)
                     /gene="dapA"
                     /locus_tag="PLX53_00480"
     CDS             complement(5428..6309)
                     /gene="dapA"
                     /locus_tag="PLX53_00480"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009160945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOY71587.1"
                     /translation="MQNDRFKGLGVAMVTPFSSSKAIDYSATDRLLEYLVDGGVDYLV
                     VMGTTGEASTLTDDEKHELIAHVVEKNNGRLPIVLGVGGNCTSSVISSIKHFNLKGVD
                     AILSVTPYYNKPTQKGLEMHFRAVAESSPLPLILYNVPGRTGVNMSAQTTLTLANEVP
                     NIIGIKEASGNLNQMAYILRDRPDNFLVISGDDGLALSQMAMGADGIISVVGNAVPGR
                     FAEMVHFAQKGKFAEAAVIHLKLVELIDALFVEGNPGGVKAALSILGIIDNNLRLPLV
                     PVGEATLQKLNMLLKSL"
     gene            complement(6330..6893)
                     /locus_tag="PLX53_00485"
     CDS             complement(6330..6893)
                     /locus_tag="PLX53_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71588.1"
                     /translation="MFDDYKYKKALNEINRQAQGFKRQRMVHNLTTARKILIVFTLNS
                     QEVIDSVLNLVKYFESLNITVHALGYYPQKEVPQVFTMHPGITIFSKAETNWYGKPLS
                     SDVEAFINKEYDILIDVDFDEVVPVRWISTFSKAKFKLGSQNYLNNPFDLILTVDKLK
                     GLPYLCEQVKQVLFKLNNRFADEPHQA"
     gene            complement(7140..>7422)
                     /locus_tag="PLX53_00490"
     CDS             complement(7140..>7422)
                     /locus_tag="PLX53_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOY71589.1"
                     /translation="GLKLQVEQIENLQLSDALKGMSVVISGTFSKVSRDELKELIRQH
                     GGKNVSSVSASTSLLIAGNNIGPSKLEKATKLGVKIVGEDEFFKLIGQE"
BASE COUNT         2114 a         1666 c         1551 g         2091 t
ORIGIN      
        1 atgaaataat taatacctgt taccacgagg caaaaatgtt actaaaagaa aaaaagcatc
       61 ttctggataa aatggcctcc attcttctaa aacaggaggt gattaatcac gatgaaatca
      121 ttagtattct tggagataga acaccaatcg aggattaaag caaatgttta ccgcttaact
      181 tctatggctg aattttttca tttattgccg ccaacatccg gttgagctct atcacctcaa
      241 taggcctcag ggagctaaga gcctcagaaa gaacatccaa aataatgctc tcggctttag
      301 gcttaaaatt cgaggctgct ttcgtagagc taaccagctc cgatcggcta ttatcagggt
      361 tgggaaggcg aacaaccaaa tcgagacgtt ccatacatgc caaggttcgt gaaagtgaac
      421 ccttatccct tcctgatatt tgggccaaat gagcctggct gagtggttct cttgaaaaca
      481 aaagggtaag aatgtaccag taatcaacgg taaagtcatc accaaaagct tccgatagcc
      541 ttcgggtaat ctcgcctctt atggcaaccg atgtgcgatg tatgttgaat ggcaggttta
      601 aaagatcttc attaaaatca tctctcataa acaagtgaat attgtgttag ccaagccgtg
      661 aatagtccgc ttaaatttaa taaaatattc acaacttatt ttccactcca gcatttatcg
      721 gcattcgtaa atttgtaaca ccattagttt aaaccaataa taaggtttta gctctaattt
      781 aatgcaagca gcaagtaata aaatgcttca caataaagtt tttatatagc attaacggtt
      841 tttaccttac tagatgttta accaccattc tttaaaattg atgaattttt ataggagctt
      901 attaacccca ttagtaggta ttttgatttt atcgagctgt gttacccaac taacgcccgg
      961 ggtttcgaaa tcacttgccg actctcgcag tgcaaacata aaaaatttgg aataccggtt
     1021 atatttttcg atacccaacc ggttaagttc acctgttccg ggccgagccg agcttaactt
     1081 tgaacttatc aaccggaaag atttggttat tgatttcatg accgaagcca ccaacctgca
     1141 atcaattacc gtaaacaacc ggctattgag cataagggtt aaaaacggcc acatcaatat
     1201 tccatccaaa tacctgttaa ccgggcaaaa taaagtaaat atagattttg tatcgtgttc
     1261 cccatcgctc aatcggaaag ccgactacct ttacacgctt ctggttcccg acagggcatc
     1321 aactgttttc ccatgctttg accagcccga cctgaaggca gctttcaacc taacgcttga
     1381 aattcctaag gaatgggttg cagtggcaaa cgccccatta ggcaaaatta ttgagtcgga
     1441 tacatcaacc gtttatgttt ttgaaaaaac caagccagta agcacctacc tgtttgcttt
     1501 tgcctgtggt aagtttaaag tagcttcgca accaaccaga tatggcgaga tggccattta
     1561 ctatcgcgac aacgattctg ccaaggtaaa tcgtaatatt gaagccattt acgagttgca
     1621 cacccaatcg ctgggctgga tggaaaacta tacggccatt ccatatccat tcagcaagct
     1681 ggacatcgtg cttattcccg attttcagta taatggaatg gagcatcctg gagcaatcta
     1741 ctaccgggaa tcaagcatct tgctcgaagg cgatccgtca gaaaacaagc tgcttaatcg
     1801 cgcaaacctt attgcccatg aggtctcaca ccagtggttc ggggacctgg ttaccatgcg
     1861 atggttcaat gatgtttggc taaaggaggt attcgcaggc ttgatggccg ataaaattgt
     1921 caatccccag tttcccgagt tcaaccacca gcttagcttt ctgctatctc actacccacg
     1981 tgcttattca gttgacagaa cggctggagc taaccctata cggcaggaac ttgacaacct
     2041 gcttaacgcc ggctcgcttt atggcgatat catttaccat aaagctccca ttgcccttat
     2101 gcagcttgaa atggctatgg gtgttagtgc ttttcaggag ggattaagaa agtatctgaa
     2161 tacctatagc atgggaaacg ccagctggga tgagctgata gacattcttg acccgttaac
     2221 ctcactaaat ttaagtgtat ggagtaaggc ttggattgaa agtgttggta tgccaaccct
     2281 taaagcaacg ctaaacgata gcatgtacgt agtaaaaaaa atcggcgtac agcttccaat
     2341 ggtttacagc gtggaggttt tatgcggtag caatgtaaac gaggctaaac ccattcagca
     2401 tattcaacct gaagtttcta cactatttgc aagtagctgt gagctagaaa gagtgattgt
     2461 tccaaatgct gacgggcttg gttacgggat gtttgttccc gattcgcagt cgcttaaact
     2521 actgtgtgat agttcttttc gaatagctga tccggttacc cgttcagcat ccttgatatc
     2581 ggctaacgag ctatttttaa acggcaaact tgatggaaat acctacttca acatgctact
     2641 tgcctcactt gcaagggaaa acgaacctca agtgcgcagc tacatccttg ccagtattga
     2701 aacctactgg tggacttttc tggcggatag cgccagggaa aatttatcgc aacaggttga
     2761 aattcaattc tccaagttgt tactaaacag ttcaatccca gctgatgagc gaaagtcaat
     2821 tttcaagaca tacgccagga tagctgtgtc cagcaatgcc gctgaagagc tgtatggaat
     2881 atggagcggt aataaatctt taccaggttt aaccttcacg gagaccgatt taacctttct
     2941 agcctacgag ctagctgtta gaaatgtgca taatgccgac agtattctgg atctacagga
     3001 aaaaagaata gccaaccccg acctgcttgc aaaattccga tttgttcgac gcgccctaag
     3061 tcccgatgac gccttacgcg acgacttttt ccatagcttg ctgattcctg aaaaccgacg
     3121 acctgaaccc tgggtggctg aagcccttca ctactttaac catcccttaa gggatagttt
     3181 tgccagccaa tacctaaggg cttcattgga tttacttccc gagattcagc aaacaggaga
     3241 tatcttcttc cccaaaactt ggcttgatgc cattctatgg ggtaaaaaca gcaaccaagc
     3301 tgctgaaatt gtaagcagct ggctaaacca gcaccctaat gtacccgata acctgaaaaa
     3361 taagcttatt caatcgtcgg atttgctatt cagggctgtt gataaaagga agaaaaaata
     3421 gtttccacaa actaaccaaa aaaaaggcta ctcgctgccc ggaggcagag gtagtctttt
     3481 aaaaggatat gctcctaatc tgcttacttc acaaactgga gggcatagta ctttgccgtt
     3541 agcttttcgc cagtggcctt ctcaatcatt tcgttccagg gatacaggca tcctggcaca
     3601 aaaacctttt ccttgaggta tgcacccact tcggggtgtc ccacaaaact agccgcatta
     3661 aaatcactaa tctttagcac gttggcagct atgtaggcat taagctgtga ggcaagcatt
     3721 tctcccatca agtaattatg atagtagcat gggtagaggg caacatgaat ttttgaagcc
     3781 caatcgggtt cgttacgtcc ttcaggtttt ttcaacagct ggtacttttc aaccatgctc
     3841 caccaaaggg aattcaaatc ctgatcggga ttggcgtaca tctccttttc gaaacggtac
     3901 atcacctgag cccagcggct aaacacgagc tgctcaagcc taagggattt taggcattcg
     3961 gaagcaattt gttctttttg ttcaggggtg ataattccca tatcgagcat ccactggggg
     4021 ttgcttgcca gacgtcccat catttcggca attgcctcgg ttgtaaagct atgagcggca
     4081 tctcttagga agtaaggttt tgcgggatcg ttatacttgg agtaaacggc atggccattc
     4141 tcatgcaaaa gggtattcat ccaaccatag gttggcttca cgttgcaaag caccctaaca
     4201 tcgcccgaac ggtcaatatc cgtgcaatag gcatgttgat ttttcccggg tttctcgtag
     4261 agatcggaac gagctattat gtcgttaatg ttaaggccaa ttccgttgta gaatttggct
     4321 gtaagtgcaa ccacatcctt gtcgctgtaa aattggtcga gattaacatt gtaaattttg
     4381 ggagcttcct gaaagtacct attttggtaa tgccagggca tcaacttgtc gggggaaact
     4441 ccaaagcgtt tagacaagta ggagtcaatt tcgagtttaa gctcggaaaa cgacccagag
     4501 gttaagctgt cgagctcatc aaaaagtgcc aacacttcgt cgggatcttg ttcagagaga
     4561 accatgttca tggtgtggta gttggcatag cccaattttt gggcaatctg gttgcgtacc
     4621 ttaaccagct caatgatttc gggggcaacc tcggggccaa tttttttgtg cgccttccag
     4681 gcactctcca cctcgctgct ttgggtcgaa gtggcaagta tgtcttctat ttcgttatcg
     4741 gttagctgct tgctgccaat tttagcccta aagttggagt acttcttctc aaggttggcc
     4801 tccatggcaa tttctttctc gagtagggtc gggtcaacct gattacgaag aaaggccagg
     4861 taaagaatgt ccatttgacg agaaagcacc gaatccttta ccaatccgga ttctttaata
     4921 ctcttaagct ctgcaaaaag cgaggtgtct gcaaaaattt taaccagctc aacctgggct
     4981 ttctccgatc gctggtaatc ttcatcctta cccgatatgg aggcatccca cgaagctata
     5041 gcagcttctt tggataaggg aattaccttc gactcaactt ttgcaataaa gtcggccatt
     5101 tgtttttcca tttttttctg attagatgtg catgatgagg cagctacaag cacaatcatc
     5161 agcatcactg gcataatagg ttttatttta ttcataaggc aataattttg ttgactcaaa
     5221 attagtaaac ctttaaacga tatataacca caatataaaa ttatagcgta aaacttgatg
     5281 tgtgaaccgg cagcctatca gcaaattcaa aataataagt tggttgtgtt gccgaactat
     5341 aaactccgta gtaaaataga agttaatgga caccaacaag aagttagcgg gtgcggtctt
     5401 gtagctataa aaacattagc tcggctgtta aaggcttttc agcaacatgt taagtttctg
     5461 aagtgtggct tcgccaaccg gaacaagggg taaccgaagg ttgttgtcga taatgccaag
     5521 tattgacaat gccgctttaa cccctccggg atttccctca acgaaaagag cgtcaatcag
     5581 ctccaccagc ttcaggtgaa tgacggcagc ctcagcaaac ttgccttttt gagcaaaatg
     5641 aaccatttcg gcaaaacgtc ccggcacggc attgcccaca accgaaataa taccatcagc
     5701 acccatggcc atttgtgata atgccaagcc atcgtcgccg gaaattacca aaaaattatc
     5761 gggtctatcc ctcaggatgt acgccatttg gttgaggttt cctgaagctt cttttatacc
     5821 gataatattt gggacctcgt tggccagggt tagcgtggtt tgggcactca tattaactcc
     5881 tgttctaccc ggaacattgt agagaatcag gggtaatgga gagctttcag ccacagctct
     5941 gaagtgcatt tccaacccct tctgggttgg cttgttgtag tagggcgtaa ccgaaagaat
     6001 ggcatcaaca ccctttaggt tgaagtgttt tatggagcta atcacgctcg aggtgcagtt
     6061 accgccaaca cctaaaacaa tgggtaacct gccgttattt ttttcaacta catgcgcaat
     6121 taactcgtgc ttttcatcgt ctgtcagggt cgaggcttca ccggttgtcc ccatcacaac
     6181 caggtagtca actcccccgt caaccaaata ctccagcaac ctatcggtag cggagtagtc
     6241 aatagctttg ctgctgctga aaggggttac catggcaacc cctaatccct taaatctatc
     6301 gttttgcata atagtcggat aagctagttt taagcctgat ggggttcatc ggcaaaacgg
     6361 ttgttgagct taaagagaac ctgttttacc tgctcacaga ggtatggcaa tcccttaagc
     6421 ttgtcaaccg ttaaaatcaa atcaaacggg ttgttgaggt agttttgaga gcccagcttg
     6481 aatttcgctt tagaaaatgt cgaaatccat cgtactggaa caacctcatc aaaatctaca
     6541 tcaataagaa tatcatactc cttgttaatg aaggcttcaa catcggagga taagggtttt
     6601 ccataccagt tggtttcggc cttactaaat atggttatcc ctgggtgcat ggtgaaaact
     6661 tgtggcacct ccttttgggg gtagtagcct aaagcgtgga cagttatgtt taaactttca
     6721 aaatatttaa ccaggtttaa cacgctgtca ataacttcct gactgttaag ggtaaatact
     6781 atcaaaatct tacgggcagt agtgaggtta tgcaccattc tctgcctctt aaacccttga
     6841 gcttgcctat taatctcgtt cagagctttt ttgtacttat aatcgtcaaa cataccttcg
     6901 cattctagct tatcaataat aataaaagtg ctattaaccc gctacttaat tctaaaactc
     6961 ggcaaatttg gaaggataat ctaaaaggca attttacaaa aaaatgggtt gataatcaaa
     7021 tttaacaaag ctttacaaag tcggagggca accgcttgaa aattgacaac cctgaagctt
     7081 ccaatcccaa aatagtttag tgcagccagg ttgttgcctc cccccaaagg tagaatgcgc
     7141 tactcttgac caataagctt aaaaaactca tcctcgccaa ctattttaac tcccagcttt
     7201 gttgcctttt caagtttaga aggtccaata ttattgcctg caattaaaag ggaggtggaa
     7261 gccgaaacgc tgcttacatt tttcccgccg tgctggcgga taagttcttt taactcgtcg
     7321 cgcgaaactt ttgaaaatgt tccggaaatg actaccgaca ttcccttaag cgcatccgaa
     7381 agctggaggt tttcgatctg ctctacttga agttttaatc cc
//
LOCUS       DAOOQX010000013         7183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_12807_length_7183_cov_3.354658, whole genome shotgun sequence.
ACCESSION   DAOOQX010000013 DAOOQX010000000
VERSION     DAOOQX010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12807_length_7183_cov_3.354658"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2223)
                     /locus_tag="PLX53_00495"
     CDS             complement(<1..2223)
                     /locus_tag="PLX53_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOY71590.1"
                     /translation="MSEENSEQSYLNSYQLDVYDTSFDKLMQKRIYKVLIICSSYDAF
                     MLEEDGRIDEQIFNEYVSLNLRYPPVFIQANTAREALRVLQEEKIDLIISMLNVGEID
                     PFRLAKIFKGKYPNVPIVLLTPFSREVSLQIQKEDLSSIDYVFCWLGNADIMLAIIKL
                     IEDKMNADHDILEIGVQAIILVEDSIRYYSSFLPNIYKIVFRQSRSFMKEALNEHHRM
                     LRMRGRPKILMATNYNDAIALYNRYRNNVLGIISDISYKVDNKRDHLAKSGLRLCRVI
                     RENDPNIPFLLQSSDRNNKVYADEMGVGFLHKYSKTFSIDLRNYMINNFGFGDFVFRD
                     PDTLAEVARASDLSQLQQLILTVPDRVLVYHTSRNEISRWLYARAIFTIAELFKPMTL
                     KDFNSIDDVRSFLYKAISAYRLSKGRSLIANFDRNTFDEYKFFTRIGEGSIGGKARGL
                     AFINQVIRKHRLFNKYEGIIISIPRTVVVSTEIFDEFMESNNLYDTALQDISDENVLK
                     AFLNARLPERLKVDLRKVISVFTRPLAVRSSSKLEDSHYQPFAGIYSTYMVPRSSDSE
                     KMLYMVCQAIKAVYASVFYRSSKAYMNATQNVIDEEKMGIVIQEICGNEYGSWYLPTL
                     SGVARSINFYPVGSEQPFDGIASIGFGLGKLIVDGGVALRFSPKYPKKILQLASPETA
                     IRQTQKYFYALNTDPDTFEPMVDDGMNLLKLEIKDAYSIPDLRHTVSTYDMQSGMLRD
                     G"
     gene            complement(2455..2877)
                     /locus_tag="PLX53_00500"
     CDS             complement(2455..2877)
                     /locus_tag="PLX53_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOY71591.1"
                     /translation="MMRRRSTLPSIVDEFFGDDLWNRFFDDTESVTVPSVNIKEGKDE
                     YAIEVAAPGFDKKDFRIDLNNNVLEISSEKEVKEENKDEKVMRREFRYSSFKRTFTLP
                     DTVDTEKIKASYKDGILSISVPKKDEAKVKPTKQIAIS"
     gene            complement(3170..3850)
                     /locus_tag="PLX53_00505"
     CDS             complement(3170..3850)
                     /locus_tag="PLX53_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOY71592.1"
                     /translation="MKRLLFISLLLATAVVDVKAQYSRASLWTSDMERFAREDSTHGC
                     KANCVLFVGSSTFTRWTTAVADFPESNILNRAFGGSTLTDLIYYFDQIVLPYNPLQVV
                     IYEGDNDLAETSKSVGEFIDDVTTITRLVNIHFPRAQILLVSIKLSPSRKEYFSKYLK
                     ANELMKQMADSSEYIDFVDTCTPMLNPDGSPNEDFFGSDLLHLNEKGYLLWKSLLYPR
                     LMKAGQKE"
     gene            4187..>7183
                     /locus_tag="PLX53_00510"
     CDS             4187..>7183
                     /locus_tag="PLX53_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOY71593.1"
                     /translation="MVEKASKTKSIARDSALGEEQGVVEFDKLKSLVLQNESSIYVVQ
                     SFRVVFCNQSFSDLTGYTEQEVKEILFLDIVHPKDRKLIKILFQNDFQEIRQHKSNSF
                     TLRILTRDNELRLIKSHISLIAWEGKSALLCTSYDITQQKEAEENLADLEQNFGILAN
                     TFGDFIFVLNHNHNIIQANRAVIDRLGYQEHELLLESFVNLHAPSDRSTVRSLLLGVF
                     AGTHCSYSSFLLAKSGYRIPVEVRMVKGMWKRKASIFAIAQDITPRIEAEREVKASEE
                     KFSKAFNTGAVMMAICTMDGGTYLDVNEAFLQKTGLKRDEVIGQSLHDQKIYSDVNRL
                     NQLLAQIREKGKVNNVEVGISNQRGEELTLLLSAEVISIQENDCIMIAMNDITERKRM
                     EEEITNSRIQLKGILDNLPFIAWLADNKGRYLLVNKSFTSFFNTDEGRVLGKKHGDFW
                     AKAHSANLRKHEVEAAKAKKSLSWEAFEKNDLQEEWWEHFVTPVINAENEVIATTGLA
                     RIITDQKLIRLQIEQNLERQKFLTEISYLFNTGVKFETKLNQSLQKVSGLLKLKRAFI
                     VERIHSTTPSPKVFEWHDSTANLDQDALISSLDKYSPAIFDHLNQTSQLVADICSTEE
                     PSVNSLSKALSAKNLFIYPLISEGCCIGFIGVEYSSYNPQTTGNNREFMLTFSSILTA
                     ALEGQSKESKLLESEQRFRTFSELLPEMVFEANDRQVIVFTNNNLLEAFGFSKRKLPA
                     SLKIHDLFMASEHDKLTNHLAKSAQNGQQQSAELLAQRRDKTTFPTLTHVRRVKLSKS
                     KYRYIGVMVDITMQKQQEAELLKAKEQAEAAGRAKERFLSTMSHEIRTPMNAIIGMTN
                     ILLEENPATSQLVNLNALKFSAENLLTLLNDILDFSKIEAERVDLLINETDIHSLHAG
                     ILSSYAQLAAQKGIKLTGSISPLIPHKLLANRVRLNQILTNLVGNAIKFTSNGGVDLS
                     ISLQRQLKKSVWVKFTVTDTGIGIPKD"
BASE COUNT         2067 a         1634 c         1621 g         1861 t
ORIGIN      
        1 cccatcgcgt aacatgccgc tctgcatgtc gtaggttgag acagtgtgcc tgaggtcggg
       61 aattgagtag gcgtctttaa tttcgagctt gaggaggttc attccatcgt ccaccatggg
      121 ttcgaaggta tccggatcgg tgtttagggc gtaaaagtat ttttgggttt gcctaatagc
      181 tgtttcgggg gaggcaagct gaagaatttt tttggggtat ttcggtgaaa atcttagggc
      241 aactcctccg tcaactatga gcttccctag cccaaaacct atgcttgcaa ttccatcgaa
      301 cggctgctcc gacccaaccg ggtagaaatt gatgctgcgg gctactcccg aaagcgttgg
      361 gagataccag ctgccgtatt cgttgccaca aatttcctgg ataacaatgc ccatcttttc
      421 ctcgtcaatt acgttctgcg tggcgttcat gtatgcctta ctggagcggt aaaataccga
      481 ggcatacacc gctttaatgg cctggcaaac catgtaaagc attttttcgg aatcggagga
      541 tcgcggcacc atataggtgg agtaaatgcc agcaaagggt tggtagtggg aatcctcgag
      601 tttgctcgaa gatcttacag ccaatggccg tgtgaaaacc gatataacct ttcggaggtc
      661 aaccttaagc cgttcgggta gccgggcgtt cagaaaagct tttaaaacgt tttcgtccga
      721 gatatcctga agagctgtat cgtagagatt gttggactcc atgaactcgt cgaagatttc
      781 ggtgctaacc accactgtac ggggtatgct tatgataatc ccttcatact tgttaaaaag
      841 cctatgtttg cgaatgacct ggttaataaa tgcaagcccg cgagcctttc cgccaattga
      901 accctctcca attcgggtaa aaaatttgta ctcatcgaag gtgttcctgt cgaagttggc
      961 aatcaggctc cttcccttgc tgagtcggta ggctgaaatg gccttgtaaa gaaagctgcg
     1021 aacatcatca atagagttaa agtccttgag ggtcatcggt ttaaatagct cggcaatggt
     1081 aaaaatggca cgtgcgtaaa gccaccgcga tatttcgtta cggctggtgt ggtaaaccag
     1141 caccctgtcg ggaacggtta ggatgagctg ctggagctga cttaggtcgg aagcccgggc
     1201 cacctcggct agggtgtcgg ggtcacgaaa aacaaaatcc ccaaaaccga agttattaat
     1261 catgtagttc cgcaggtcta tgctgaaggt tttcgagtac ttatgcagga aaccaactcc
     1321 catttcatcg gcataaacct tattgttcct gtccgatgat tgcagcagga agggaatgtt
     1381 gggatcgttc tcacgaataa ccctgcataa ccttaatccc gacttggcca ggtggtcacg
     1441 cttgttgtca accttgtagc taatgtcgga aatgataccc agcacgttgt ttcggtaccg
     1501 gttgtaaagt gcaatagcat cgttatagtt ggtggccatc aggattttag gccgaccacg
     1561 cattcgcagc attcggtggt gctcatttaa cgcttccttc ataaagctgc gcgactgcct
     1621 gaacacaatt ttatagatgt ttggcaaaaa tgaggagtag tagcgaatgg aatcctccac
     1681 cagaattatt gcctgaacgc caatttcgag tatgtcgtgg tcggcattca ttttgtcctc
     1741 tataagctta attatggcca gcataatatc ggcattgccc agccagcaga aaacgtaatc
     1801 aatggagctt aaatcctcct tctggatttg aagggaaacc tcgcgcgaaa agggggtaag
     1861 gagaacaatg ggtacgttcg ggtatttccc cttaaatatt ttggccagcc taaagggatc
     1921 aatctcccca acgttgagca tggatatgat aaggtctatt ttctcttcct ggagtacccg
     1981 caaggcctcc ctggccgtat tggcctgtat gaacaccggc gggtacctaa ggttaagaga
     2041 aacgtactcg ttaaaaatct gctcgtctat tcgtccatcc tcctccagca taaaggcatc
     2101 gtagctgctg caaataatca gcaccttgta aattcgcttt tgcatcagct tatcgaacga
     2161 tgtgtcgtac acatcgagct ggtagctgtt caggtacgat tgctctgaat tttcctccga
     2221 catgtggtat tagctttcgg tcccgatgcg aatatagcaa ctttaggtga aattgttacc
     2281 ctactcattt atagcctttt gctatgcctt aatggctgga actataatgc atgaatagtg
     2341 ccgatggaag ggagtcgaag ctaaaacatg ctactataag ctggtaggaa aaagggtggg
     2401 taaataacaa aaaaacccgg ctctaagtcc gggttttttt gttgccaaca gaggttacga
     2461 aatggctatc tgcttggtgg gtttaacttt tgcttcatct ttcttaggaa cgcttatgct
     2521 taaaattcca tccttgtatg aagctttaat tttttcggta tcaaccgtat cgggcaaggt
     2581 gaaagtcctt ttgaacgagg agtacctaaa ctcccgacgc attacctttt cgtccttgtt
     2641 ttcctctttt acttcctttt cgctggaaat ttcaagaacg ttgttgttca ggtcaatacg
     2701 gaaatccttt ttgtcaaagc caggagcagc aacctctatg gcgtactcat ctttaccttc
     2761 cttaatattt acggaaggca cggtaacact ttcggtatcg tcaaagaacc tgttccacag
     2821 gtcgtcgcca aaaaactcat caacgatact tggcagtgtg cttctacgtc tcatcatcgg
     2881 catcatagct ttaccctcca tactttaagt tagacattca atttttaaac tctgatacct
     2941 aactatcaac ttgaatgcca agacgaaaaa gcgccatttt gaccgaatgg tgtcaacttt
     3001 gtctgccatg ttgacaccaa ggcgagaagg agaaagcgca caggtgaaaa atgtcagcac
     3061 cagcaacccc tattcaacaa aatcatgtcc ggttttaagc tattgacact ttgataatat
     3121 ggcaatctct gccaagcggc agatttttgg gaagcactgt aaacaggagc tattcttttt
     3181 ggccggcttt cataagccga gggtaaagca acgatttcca aagcaagtaa cccttttcgt
     3241 taagatggag caaatcggat ccaaaaaaat cctcgttggg agagccatca gggttcagca
     3301 tgggggtgca ggtatctaca aagtcaatgt attctgaaga gtcggccatt tgtttcatca
     3361 gctcgttagc cttaagatac ttactaaaat actccttacg cgaggggctt agcttaatag
     3421 ataccagcag tatttgtgcc cggggaaaat gaatatttac caagcgggtt attgtagtaa
     3481 catcatctat aaattcacca acactttttg aagtttcggc caaatcgttg tcgccttcat
     3541 agattaccac ctgcaaggga ttgtagggta aaactatctg gtcgaaatag taaatcaaat
     3601 cggtcaaagt agagccacca aatgcccggt taagaatgtt cgattcggga aaatcggcta
     3661 cagcggttgt ccatcgggta aaagtggagc tgcccacaaa tagcacacag ttggccttac
     3721 aaccatgggt agaatcttcc ctggcaaacc tctccatgtc gctagtccac aatgaagcac
     3781 ggctatactg cgccttaaca tctactacag ctgttgcaag cagcaaggaa atgaatagta
     3841 atcttttcat accaatcaaa ttcacctaaa ggttacacta ccaagtatca aaaagttgaa
     3901 tatttatagt taaactttca cattcgaaac atgttgcaaa cggggagaca aatcatttcg
     3961 ttgaaaaaac gtataattct gcaataggaa tccaatttga tgataagaac gcgggttgaa
     4021 atgtaacatt tccacattac aaatatacct ttttggaatt tcaacatacg aacgcaggaa
     4081 caagctattt tcctagtata tcaaaaaatt tcagatcatg taggaattgc tctttactat
     4141 tggcaaactt tttaatacct tttggcaatt ttttagtaac cactaaatgg ttgaaaaagc
     4201 ctcaaaaacc aaaagtatag ctagggactc cgctctcggc gaggagcaag gagtggttga
     4261 attcgacaag ctgaaaagct tggttcttca gaacgagagc agcatatacg tggttcagag
     4321 cttccgtgta gtcttttgta atcaaagttt ttccgactta acgggctaca ccgaacagga
     4381 ggtaaaggaa atactattcc tggacattgt ccatcccaag gatagaaagc taatcaaaat
     4441 attattccag aacgattttc aggaaatcag gcagcacaag tcgaacagct tcactcttag
     4501 aattctcacc cgcgacaacg agctgaggtt gataaagtcg catatctcgc taatagcgtg
     4561 ggaaggaaaa tcggcgctgc tatgcaccag ctacgacatc acccagcaaa aggaggccga
     4621 ggaaaacctt gccgatctgg agcagaattt cggcatactt gcaaacacat ttggcgattt
     4681 tatttttgtt ctcaaccaca accacaacat cattcaggca aatagggcgg tgattgacag
     4741 gcttggatac caggaacatg agctcttgct cgaatcattt gtaaaccttc atgctccaag
     4801 cgataggtca actgttagga gtttactttt aggagtattt gctggcaccc actgctccta
     4861 ctcatccttc ctgctggcta aaagcgggta tagaattcca gttgaggtta gaatggttaa
     4921 aggaatgtgg aaaagaaagg cttccatatt tgccattgcc caggatatca ccccccgtat
     4981 tgaggccgaa cgcgaggtga aagcatcgga ggaaaagttc tcgaaagctt tcaacaccgg
     5041 tgccgttatg atggctatat gcaccatgga tggcggaaca tacctggatg ttaatgaagc
     5101 tttcctccaa aaaacaggac ttaaacgcga tgaggtgatt ggccaatccc tacacgatca
     5161 aaaaatatac tctgatgtaa ataggcttaa ccagctcttg gcacagatca gggaaaaggg
     5221 taaggtaaac aatgtggaag ttggcattag taaccaaagg ggcgaggagt taaccctgct
     5281 gctttcggcc gaggttatca gcattcagga aaatgactgc atcatgattg ccatgaacga
     5341 catcaccgag cgcaagcgga tggaggagga aataaccaac agcaggatac agctaaaggg
     5401 aatactcgat aacctgccat tcattgcatg gctcgccgac aacaaaggtc gctacctcct
     5461 ggtaaacaaa tcttttacca gtttctttaa caccgacgag ggtagggttc tcgggaaaaa
     5521 acatggcgat ttctgggcta aagcccactc agccaatctc cgaaagcatg aggtggaagc
     5581 agctaaagct aaaaaatcat tatcgtggga ggcttttgaa aaaaacgacc tgcaggaaga
     5641 gtggtgggag cactttgtta cccccgttat taacgcggag aatgaagtga ttgccactac
     5701 tggcttagcc cgaataatta ccgatcaaaa gctgattagg ctgcaaattg agcaaaatct
     5761 ggaacgacaa aaattcctaa ccgaaatttc atacctgttc aacaccggcg taaagttcga
     5821 gaccaaatta aaccaatccc tgcaaaaggt ttccgggttg ctaaaactca aacgggcttt
     5881 tattgttgaa cgtattcatt caaccacgcc ttccccaaag gttttcgagt ggcacgacag
     5941 cacagccaac ttagaccagg atgcgctgat tagctcactt gataagtatt ctcctgcaat
     6001 tttcgaccat ttaaaccaaa ccagccaact ggtagctgac atttgctcca ccgaagaacc
     6061 gtcggtgaat tcgcttagca aagccctgtc tgcaaaaaat ctattcatct atccgctcat
     6121 cagcgagggt tgctgtatag gttttatagg agttgagtac tccagctaca accctcagac
     6181 taccggcaac aacagggagt tcatgcttac gttttcatca atactaaccg cagcacttga
     6241 ggggcaaagc aaggagagca agcttctgga gagcgaacag cgtttccgga cattctccga
     6301 gctgctgccc gaaatggttt tcgaagctaa cgacaggcag gtaatagttt ttaccaacaa
     6361 caaccttctt gaagcattcg gattcagcaa gcggaaactg ccggctagcc tcaaaattca
     6421 cgacctattt atggcctccg agcatgacaa gctaacaaac cacctggcca aatcggccca
     6481 aaatgggcag cagcagtcgg ccgagctgct tgctcaacgc cgtgataaaa ccaccttccc
     6541 cacactaacc catgttcgac gagtaaagct cagcaaaagc aagtacaggt atattggcgt
     6601 gatggttgac ataaccatgc aaaagcaaca ggaagccgaa ctgcttaagg ctaaggagca
     6661 agctgaagcg gcaggccgtg ccaaggagcg gtttctttcg accatgagtc atgagatacg
     6721 aacacccatg aacgccatta ttgggatgac caacatcctg ctggaggaaa atcctgcaac
     6781 cagccagctg gtaaacctga acgcccttaa attttctgcc gaaaacctcc ttaccctttt
     6841 aaacgacata ctggatttca gcaagataga ggcagaacgg gtagatctcc ttataaatga
     6901 aaccgacatt cactcgcttc acgccggaat attgagctct tatgcgcagc tggcggctca
     6961 aaagggcatt aagctaaccg gtagcatcag ccccttaata ccgcataagc ttttagccaa
     7021 tagggtacgc cttaaccaaa tactcaccaa cctggttggt aacgccatta agttcacctc
     7081 caatggaggc gttgacttaa gtatttcgct tcaacggcag cttaaaaaat cggtttgggt
     7141 taagtttaca gtaaccgata cgggcattgg aatacctaag gat
//
LOCUS       DAOOQX010000014         7155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_12875_length_7155_cov_2.992535, whole genome shotgun sequence.
ACCESSION   DAOOQX010000014 DAOOQX010000000
VERSION     DAOOQX010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12875_length_7155_cov_2.992535"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            428..646
                     /locus_tag="PLX53_00515"
                     /pseudo
     CDS             428..646
                     /locus_tag="PLX53_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617848.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
     gene            1002..2303
                     /locus_tag="PLX53_00520"
     CDS             1002..2303
                     /locus_tag="PLX53_00520"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY71594.1"
                     /translation="MNNKRLQPYAVYSDGKGNVYEDRSLFAVGRSGHEFYPLYLDEMI
                     PLPEGSDLFELPGRKTVGYSRNGKLTARQSGLAAAAFIAPAHTQLSVAAWENKVNAPV
                     LPLFAYTAVGWYANRFYVPAIRIDLDTRQDCENFDQQVVVERGKALLEQHEGNRLVNH
                     LVENCAFTYLCPAARNMVMGRWEAPIPVSPTCNARCIGCISKQDQQESPIPETQHRLT
                     FVPTVGEIAGYAVPHLENAPNAVVSFGQGCEGEPLMQWELIRDAIKLIRSKTQKGIVN
                     LNTNGSKPRAVEELFKAGLDSIRVSMNSAQPKWYESYFRPVGFSQNDAIESLTIASRY
                     NRWASINYFVMPGFTDTAEELTALKNLIEKTDLNMIQWRNLNIDPDWLFGKTNFTGNS
                     KPLGIRYVMDEIHRSHPSVYFGYFNPHYEVMHRWQQQTGNS"
     gene            complement(2405..2608)
                     /locus_tag="PLX53_00525"
     CDS             complement(2405..2608)
                     /locus_tag="PLX53_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71595.1"
                     /translation="MSSKDIRVIFRLHRKVWYAWCLSTFPGVELKINRVAINTAGSYQ
                     NPLYLKKSIFSTLSGDVVQMFYF"
     gene            2665..4161
                     /locus_tag="PLX53_00530"
     CDS             2665..4161
                     /locus_tag="PLX53_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOY71596.1"
                     /translation="MGSIQLELALLILSTLFFLSILAGKVSSKYGVPALLLFLIVGML
                     SGSDGLGIQFENIHISQAIGTIALCVILFSGGLDTQFSEIKPVLAQGLILATLGVLLT
                     AIFTGLIIWWILDLTIPGANIGLLTSLLLAATMSSTDSASVFSILRSKGLNLKKNLRP
                     MLELESGGNDPMAYVLVITLIDIIKMNSEPNFWLASGMIVLQLIIGGGVGYLLGKLAI
                     ITINHIKLGNDSLYPILVFTFCIFIFSITYFLRGNGFLAVYVGGLVIGNAKFVHKRSS
                     LNFFDGLAWLFQLVMFLTLGLLVNPHELIPIILPALAVSIIIIFITRPISVFICLLPF
                     RKLSFKDKSFVSWVGLRGAVPIIFAIYPLVENVPHARIIFNIVFFCTLISLLVQGTSI
                     PLVAKWIGVIEKPERFSKLKPFNMDFADNLKTVSTEIEITDKMLLRGNQIMNLELPDN
                     TLVVMVKRGDNYFLPSGSTTLKEKDKLLIITDNQEALVETYRNLGIDG"
     gene            4357..5949
                     /locus_tag="PLX53_00535"
     CDS             4357..5949
                     /locus_tag="PLX53_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOY71597.1"
                     /translation="MKRIITLLFVLAGLMAHSHAQSDSVIQKIIQIGTAENQTMKHLD
                     VLCNQIGGRPVGSSAYEDAVVWAARQFQAWGMDVFLDEVDELPVGFNRGPWFGRLLGD
                     HSMTLHFATPSYTAGTKGVQRGHVVAEPKTQREFERMKGKLKGAWVLISGESNGWPID
                     YSVRANHRRDSIIASNNEIEKKNDEIQRENWMNRSANQPPKELIPLTEEPGLFYRQMV
                     EAGILGIIQSAPNPIRVLYDRKNINSMVWDSLPQVPDIKLDENQYKIIERMARERQYF
                     QLEFDIRNHFRPGPIPYHTVVGVIKGTEFPNEYVIMGGHLDAFDVATGAADNASGVAP
                     AMEAARLIMKAGGKPKRTILVCLWAGEEFGLLGSTSWVIKNSDKLQSISAMFNRDGGP
                     TVASNLSVPKAMLNDFSGICSPLNSINPSFPFTLKEATPRPKPKNPGGTDTAPFLMAG
                     VPAFQFGTEDVDGLDFSYQEIWHTERDTYEKCPADYMNHTSIVTAIVVYGVANLDHLL
                     SRENLFAPDDKPKVNGKAKGKK"
     gene            6124..>7155
                     /locus_tag="PLX53_00540"
     CDS             6124..>7155
                     /locus_tag="PLX53_00540"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOY71598.1"
                     /translation="MAVGCTLNAAAQEFDSVIIKQHVYTLASDSLMGRGFGTTGGKMA
                     ADYITAQLEAAGVMPWQGSYLHPFISSGIMVKTEGANIIGWVEGTDPLLKDEYIVIGA
                     HYDHLAYKITDDGKRIVFNGADDNASGVAAIIEIGRWLAGSRNQLKRSVILAAFDGEE
                     AGLVGSSFMVQHNQVPPKKVKIMFSLDMVGMLSRYGGIDLNGDKTLENGDVMLHNLAS
                     KHGLTVKKDGKNVVPNTDTAPFGNQGIPAIHIFTSTVSPYHKPEDDAEKLDYPGIAAI
                     SGFVADAAVQLSNAETLEPSRRFVAQQNTKLPKFGLRLGFGTSGQNYSNEFYMAKNIF
                     AFQAGFYAKQ"
BASE COUNT         2082 a         1635 c         1574 g         1864 t
ORIGIN      
        1 tatatccttc acgtagcgcc ctgatagtga tggcacgaat ggcgggattt aatcccggga
       61 cgtctcctcc cccagtaaga attccaatga ccagcttaga ttttgattcg ttttccatga
      121 ttttcaattg gtttatgacg ggtcaattat caaataccta gcaaatatat gttttatatc
      181 ataaatttca ttggtttacc aatgttaaat tttcaccacc cacaacataa acccatttcc
      241 aaaataatcg ggatggtagt atattcaact acaaatgctt ctccacaaga aaaaattacc
      301 aaaacggtta gcaccggtca tacccatccc taatgttaat tagccagata ttacggcata
      361 acctactata gattgaatat caataactta taaataattg agtatgtagg gtgattattc
      421 cccgccctct cacacctccc ttttcagcct gattactttg cctgttgagt tcgatgcatc
      481 gcgtagggcg ctccagtggc taggcttaac cggaattcag tacggtcaaa atgtagttta
      541 cgccagtgat gcactaaaat gggcagccct cacgtatgtt gttgccgcct taggctcgtt
      601 ggcaaccctt ttcttctacc ttgggtttct aaacaataac gactaggaga agcaccaagg
      661 aaactcaact aaacatagtt ctaataaaaa aggtcggcat ataaccggcc ttttttattt
      721 atgatagata atcaggctta cattatggca taaaattgat ccttagccgt tccacaaagc
      781 tcgcaatttg ccttcacatc atttttatca tacaataatc ataatgcatt aagcaacaag
      841 cacaaacact ctcagctaca gctgaacaaa gtatgtgttg aaagcttttg tttgaggaag
      901 ttacttacct taaagcatgg aagcataaat aaatgatttt ttaaccaaaa ttttacggct
      961 tgcgaaaaga ggggtatttt tacaggcaat aaataaagaa aatgaacaac aagcgacttc
     1021 aaccctacgc tgtttacagt gatgggaagg gaaatgttta tgaagatagg agcctatttg
     1081 cagtaggacg ctctggccat gagttctacc ctctctacct cgacgaaatg atacctcttc
     1141 cagagggtag tgatttgttt gaactcccag gtcgtaaaac ggtcggatat agccgtaacg
     1201 gcaagctaac agccaggcaa agtggattgg ccgcggcagc ttttatagcg ccagcacaca
     1261 ctcagctttc tgtggccgca tgggaaaaca aggttaatgc acctgtacta ccgctattcg
     1321 cctatacggc agttggctgg tatgcaaaca ggttttacgt tcccgccatt cggatcgacc
     1381 tcgatacccg tcaggattgt gaaaacttcg accagcaagt agtggttgaa aggggtaagg
     1441 ctttgcttga gcaacacgag ggaaaccggc tggtgaatca cctggtagaa aactgcgctt
     1501 tcacctatct ctgtcctgct gcacgcaaca tggtgatggg acgttgggag gcaccaattc
     1561 cggtatctcc cacctgcaac gcccggtgca ttggctgcat atcaaagcag gaccaacagg
     1621 aatcgcctat tcccgaaaca cagcacaggc tcacctttgt tccaaccgtt ggcgaaattg
     1681 cgggctatgc tgttccccat ttggaaaatg ccccaaacgc cgttgtgagc tttggtcagg
     1741 gttgcgaagg cgaaccgctg atgcagtggg agctaatacg cgatgcgatt aagctcataa
     1801 gaagcaaaac ccaaaaaggc attgtgaacc tcaacaccaa cggcagcaaa ccccgggcag
     1861 tagaagaact tttcaaagca gggctggata gcatacgggt aagcatgaac tcagctcagc
     1921 ccaaatggta cgaaagctac ttccgtcctg ttggctttag ccagaatgat gccattgaat
     1981 cgctaacaat tgccagccgg tacaaccgtt gggcatccat caactacttt gtaatgcctg
     2041 gctttaccga taccgcagaa gagctaaccg ccctgaaaaa cctgattgaa aaaacggatc
     2101 tgaacatgat tcaatggcgc aacttaaaca ttgaccccga ctggctgttt ggtaaaacca
     2161 actttacagg caacagcaaa cccctcggaa ttcggtatgt aatggatgaa atccatcgta
     2221 gccacccctc ggtttacttc ggatacttca acccccatta cgaggtcatg catcggtggc
     2281 aacagcaaac tggtaatagt taagaacaat gcaggtaaac ttttaagtta atgggatgat
     2341 tgattttagt agtacttctg agtatgggta atttttgtat tgaagtttta agtaattcta
     2401 tttactagaa gtaaaacatt tgaaccacat caccggaaag ggtactaaaa atcgacttct
     2461 tcaagtataa cgggttttgg tatgaacctg cagtatttat ggcaaccctg ttaatcttca
     2521 actcaactcc ggggaatgtg ctcaagcacc aggcatacca tactttcctg tgcagcctga
     2581 aaataaccct gatatctttg ctgctcatct tacactgtgt aaaagttgct caaaaatgct
     2641 atctttatgg gttaaaataa caaaatggga agcattcaac ttgaactggc attacttatt
     2701 ctatccacac tattttttct cagtattctt gctggaaagg ttagctcaaa atacggagtt
     2761 ccagccctgc ttttattttt aatagttggg atgcttagcg gcagcgacgg gttgggtatt
     2821 caatttgaaa atatccacat ctctcaagca atagggacaa tagcgctatg cgtcattcta
     2881 ttctcaggag gattggacac gcaattttct gaaataaaac cggttcttgc ccagggatta
     2941 atacttgcca cacttggagt actgttaaca gcaatattta ctgggttaat tatttggtgg
     3001 atactcgatt taaccatacc cggggcaaac attggactac taacctctct cctgctagca
     3061 gcaacaatgt catcgactga ctccgcttct gttttctcca ttctgcgttc aaaagggctc
     3121 aaccttaaga aaaacctccg gcccatgctg gagttggaaa gtggtgggaa cgatcccatg
     3181 gcttatgttc tggtaattac cctgatagat ataatcaaaa tgaactccga gccaaatttt
     3241 tggctggctt caggtatgat agttttgcag ctgattattg gtggcggcgt gggatatttg
     3301 ctgggtaagc tggcaattat cacaattaac catatcaaat tgggtaatga ctcactatac
     3361 ccaattttag tttttacctt ttgcatattt atcttttcga taacctattt ccttcgtggc
     3421 aacgggttcc ttgccgttta cgttgggggg ctggtaattg gaaacgccaa attcgttcac
     3481 aagcgttctt ctctaaattt cttcgacggg ttggcatggc tattccaact tgtcatgttt
     3541 cttaccctgg gattgctggt aaatccgcat gagcttattc caatcatttt accagcttta
     3601 gccgtaagca tcatcataat tttcatcacc cggccaattt ccgtatttat ttgcctgctg
     3661 ccattccgaa aattgtcctt caaggataaa tcatttgtgt cgtgggtagg actccgagga
     3721 gccgttccca ttatttttgc catataccct ttagttgaaa atgtacccca tgcccgaatt
     3781 atattcaaca tcgtattctt ctgtactctg atttcgctac tggttcaggg tacatccatt
     3841 ccactagttg ccaagtggat aggcgttatc gaaaaaccag aaaggtttag caagctaaaa
     3901 ccattcaaca tggactttgc cgataacctg aaaaccgttt caaccgagat tgaaattacg
     3961 gacaaaatgc tcctgcgcgg aaatcagata atgaaccttg aacttcccga taacacgctg
     4021 gttgtaatgg tgaaacgagg agacaactat tttcttcctt caggcagtac aacattaaag
     4081 gaaaaagata agcttttgat tattacggat aatcaagaag cccttgttga aacgtataga
     4141 aatttgggta ttgatgggta aaaaatcacc agcaacccga gctcctaaac ccgtataatg
     4201 gcatagccaa tcgcggtaaa tagtgctacc tgattttccg aatatctatc ctggagaagc
     4261 agtggtaaat cggcaattaa gtaaataaat ggaatagccg ctcgttaaac tatcagggag
     4321 caaaaaattc taatgtgaaa ataaaatgtt gttgccatga aacgaataat tacattgctt
     4381 tttgttttag caggattgat ggctcattcc catgcccaga gtgattcggt tatacagaaa
     4441 atcatccaaa ttggaactgc tgaaaaccaa accatgaagc accttgacgt cctatgcaac
     4501 caaataggcg gaagaccggt tggatcttca gcgtatgagg atgccgttgt ttgggctgcc
     4561 cgacaatttc aggcatgggg tatggatgtt ttcctcgacg aagtagacga gcttcctgta
     4621 gggtttaacc gcgggccctg gtttggcagg ctgctcggag accactccat gacacttcat
     4681 ttcgctactc catcctacac tgcggggaca aagggtgttc aacgcggaca tgttgtagct
     4741 gaacctaaaa cccaacgtga gtttgaaagg atgaaaggga agctaaaagg tgcatgggtg
     4801 ctgatttcag gcgaaagcaa cggttggcca atagactact cggttagggc aaaccatagg
     4861 cgcgatagca taattgccag caacaacgag attgagaaga aaaacgatga gattcagcgg
     4921 gaaaactgga tgaaccgtag cgcaaaccaa ccacccaaag agctgattcc tctgaccgaa
     4981 gaaccgggtt tattttacag gcaaatggta gaggcaggaa tactcggaat aatccaatct
     5041 gctcctaatc caatcagggt gctttacgac agaaagaata ttaacagcat ggtttgggat
     5101 agcttgcccc aagttccaga catcaagctg gatgaaaatc agtataaaat tattgagcgg
     5161 atggccaggg aacgccagta cttccagctg gaatttgata tccgcaacca ttttcgccca
     5221 ggccctatcc cctaccacac cgttgtcggg gttattaagg gaaccgaatt ccctaacgag
     5281 tatgttataa tgggcggtca tctggatgct tttgatgtgg ccaccggcgc agccgataac
     5341 gcttcagggg tagcccctgc catggaagcc gcaaggctaa tcatgaaagc aggaggtaag
     5401 cccaaacgca ccatcctggt ttgcctctgg gcaggcgaag agtttggcct gctgggttca
     5461 accagctggg taatcaaaaa cagcgataag cttcagtcca tttcagccat gttcaaccgt
     5521 gatggcggac caaccgtggc aagcaacctt tcggttccaa aagccatgct caacgacttt
     5581 tccggtattt gtagcccatt gaacagcatt aatcctagct tcccattcac cctcaaggaa
     5641 gcaacacctc gcccaaagcc taaaaatcca ggaggaacag ataccgctcc attcctgatg
     5701 gccggagtgc ctgccttcca atttggaacg gaagatgttg atgggctcga cttcagctac
     5761 caggaaattt ggcacaccga acgcgacacc tacgaaaaat gccctgccga ctacatgaac
     5821 cacacttcaa ttgtaacagc aatagttgtg tatggcgtag caaatctcga ccacctgcta
     5881 tcacgtgaaa atctttttgc ccctgacgat aagccaaagg tcaacggcaa ggcaaagggg
     5941 aaaaaataga ctgcgcatag ccgaagcatg ttgttaaaat tacattaatc acctacattt
     6001 acaccaggat gtggtgcagg tatgctgtaa caaaaatgca ccattaccgt cttaacatca
     6061 aactttttag ctctaaccct aagctaccaa accaagatga aatcaaaatt gattttagcc
     6121 ttaatggcag taggctgcac ccttaacgct gccgctcagg agtttgattc ggtgataatt
     6181 aaacagcatg tttacaccct tgcttccgat tcgttaatgg ggaggggttt tggaacaacc
     6241 ggaggaaaaa tggcagccga ttacatcaca gcgcagctcg aggctgccgg agttatgccc
     6301 tggcagggga gctacctgca tcctttcatc agctcgggaa taatggtaaa aaccgaaggg
     6361 gctaatatta ttggctgggt tgagggcaca gacccgctgc ttaaagatga gtacattgtg
     6421 attggagccc actacgacca tctggcctac aaaataacag acgatggtaa aagaatagtc
     6481 ttcaacggag ccgatgacaa cgcctcgggc gtagcagcta taattgaaat tggaagatgg
     6541 cttgccggaa gcaggaacca gctgaaacga tcggtaattt tagctgcctt tgacggagag
     6601 gaggccggat tggttggatc gagttttatg gtacagcata atcaggtacc acccaaaaag
     6661 gtaaagatca tgttcagcct tgatatggta ggaatgcttt cccgatatgg aggcatagac
     6721 ctgaatgggg ataaaacgct tgagaatggg gatgtcatgc tccacaacct ggcctcaaaa
     6781 catggtttaa cggtaaaaaa ggacggtaag aatgtcgtgc caaacaccga cacggcacct
     6841 tttggtaacc aaggtattcc ggccatccac atattcacct caaccgtatc gccctaccat
     6901 aaacccgagg atgatgccga aaaattggac tatcccggaa ttgcagccat atcaggtttt
     6961 gtagccgacg ccgctgtcca gctgtcgaac gcagaaacgc ttgaaccatc acgcaggttt
     7021 gtagcccagc aaaataccaa gctgcccaag tttggcctgc ggcttggttt tggtacctcc
     7081 ggtcagaact acagcaacga attctacatg gcaaaaaaca tttttgcctt tcaagccgga
     7141 ttttacgcca agcaa
//
LOCUS       DAOOQX010000015         7040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_13136_length_7040_cov_3.517108, whole genome shotgun sequence.
ACCESSION   DAOOQX010000015 DAOOQX010000000
VERSION     DAOOQX010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13136_length_7040_cov_3.517108"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLX53_00545"
     CDS             complement(<1..278)
                     /locus_tag="PLX53_00545"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOY71599.1"
                     /translation="MSISKEIKDSFRFGSSLTQLIYINLAIFLIYSLVRVIMFIGGAQ
                     PGSSYAHWLAVPAGLHQLLSRPWTLLTYMFFHENFLHILFNLLWLYWFG"
     gene            complement(465..1226)
                     /locus_tag="PLX53_00550"
     CDS             complement(465..1226)
                     /locus_tag="PLX53_00550"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOY71600.1"
                     /translation="MINTVIWLVTWMLQRYGVDLNAMLGLYTPGSPHFKPYQVVTHMF
                     MHGGFTHLFFNMFALWMFGRILEQVWGGKRFLLYYMVTGLGAAAVHSLVNYLQFSSIH
                     ADSIAMLNTLSPDTFAAFVTKHFPEFYNSIYSDFLDKWFQNPSNPIMIDQAQQLSQQL
                     LSLQLNIPTVGASGAVYGVLLAFGMLFPNTVLMILFPPIPIKAKWMVILYGGLELFLG
                     LTQAGSNIAHFAHLGGMIFGFFLIKYWQSRKNVFY"
     gene            complement(1357..3216)
                     /gene="mutL"
                     /locus_tag="PLX53_00555"
     CDS             complement(1357..3216)
                     /gene="mutL"
                     /locus_tag="PLX53_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOY71601.1"
                     /translation="MVISQMADIIHLLPDSIANQIAAGEVVQRPASVVKELLENSIDA
                     GCKAIQLIVKDGGKTSIQVVDDGIGMSETDARLCFERHATSKIKEAADLYSIRTMGFR
                     GEALASIAAVAEVELRTRRLSDEVGSMVQVGGSEFVAQQPVSCPVGSTLTVKNLFYNV
                     PARRKFLKSNSVELKHIISEFQHVALAFPEISMSLSHNGSEIYKLAPSNLKQRIVNLF
                     GKSISSQLVDCVAETSIVTISGYIGKPESARKSPGEQFFFVNGRFMKSPFLHKAVVNA
                     YNRLLPANSIPTYFIYFRVDPGSIDVNIHPTKTEVKFEDDGAVWQIVHAAVREALGKF
                     AVAPSIDFSSDPGFSIPHFPKDSPVELPEIELNPNFNPFEDEGARFAQRTREHNFTPQ
                     QPVKGWEQLYQDVNPNFNFEPKQTAVQTFASKGFDDSVAENTGRFFQIKGKYIITAVK
                     SGMLVVDQKRAHERILYEQFYKNLNLEKNVAQRELYPQTIELNPQDFETIITNQENFL
                     RLGLDISNLNHNTISINSLPGNTRITDPALFLENLLAIINENEALPFDSAKEKIALSM
                     AKAAAVGYGKRLSESEMQEVVDQLFACREPNYTADGKKTIALIEMSEFEKWLK"
     gene            complement(3216..3440)
                     /locus_tag="PLX53_00560"
     CDS             complement(3216..3440)
                     /locus_tag="PLX53_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71602.1"
                     /translation="MVNNFFFGAQEHRDTIFWVKTHKGIVRGSVDRANSAFTPERMGA
                     MWIRHPDSYLPTRNILEVLTILLLVAHLIF"
     gene            3649..4296
                     /locus_tag="PLX53_00565"
     CDS             3649..4296
                     /locus_tag="PLX53_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71603.1"
                     /translation="MKPAGAVELIILTAASMLNGCTKNVEPFPTSATLSPSLSVPLGE
                     GGVSLTKTLQTLGIPVVNLSEDVPQWATYGFVYVADTIPLNLTEVYNRGDSITYLMVR
                     TNIWNQFPLGGRAQVFFLDANNQVIDQLYEDMVSVGPAEHGSHGEVVNTAFESNETSF
                     NTSRISLLSAAQKVVIYAGLEITDEGVSESNITYFDQYQLRVQVALQVDFIMNLN"
     gene            4306..5739
                     /locus_tag="PLX53_00570"
     CDS             4306..5739
                     /locus_tag="PLX53_00570"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOY71604.1"
                     /translation="MALRRIVNLLIFFSAYSTIVCAQQDNTLFLLHNIPQSNFTNPAV
                     QIQCPVYVGVPLLSSVHFNVNSTGFSYDDVTNESGITNLNALVAGMHRWDFVSEEVHF
                     TPVSFGFMYDRHQYISFAWTERVETKVFLPKKLLSLSVDGNTQYVGDELKTGNPGANA
                     VYYREFSVGYSKKVNSGLILGVHGKLLFGLAGAFTRRRPVNVDVDAITHNIDAQWNPK
                     VDVAYPLGVATDASGNVSNVTVGGFSPLNFFLNFGNQGIAADLGFIWQHDDVTWSGSL
                     LDLGFIWWHKQTTRFENSGHFVYRGATPADLDNPDDYINQLGDSLNNQIQVEYSRKGF
                     ITLLNPKIYLGASYPVSERFNVGAHVRAEWYPGRPVMGITLSGMFFTKKGGSAALSYS
                     VMNGSFINVGAGLGWGGQTFQFYVVSDNLMAALYPEKARNANLRFGFNLFFGCIEKKK
                     KVEVPHGNGCGCYWSWDDKTRRKLGRN"
     gene            6003..6539
                     /locus_tag="PLX53_00575"
     CDS             6003..6539
                     /locus_tag="PLX53_00575"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOY71605.1"
                     /translation="MKKICRIFAIAVWMAPLGLMAQPPEVGQVAPDILLSSPTGDSIR
                     LSSLRGKLVLVDFWASWCSPCRRENPNLVQTYNKFKDACFENGNGFTIYSVSLDQKQD
                     AWLKAIADDGLVWPYQVSDLKGWRSEAAKQYGVRAIPMNYLINANGVVVATNLRGDEL
                     DSTLRKLRKWRIFSNECI"
     gene            6571..>7040
                     /locus_tag="PLX53_00580"
     CDS             6571..>7040
                     /locus_tag="PLX53_00580"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOY71606.1"
                     /translation="MKVGHILWKTAKIILITLGSLILLVVILLAAAKLSENKIVGLVL
                     NKVSVELKAPFSVREVNLLPLRNFPNLTVELQDFSVGKSTDSLQSGQSSNPFDTLFSL
                     HKLYVAVKAKPLLSSKVVVDQVEIDGATLNYHVDSTGASNFDFLMTTTDTTQVP"
BASE COUNT         1852 a         1553 c         1683 g         1952 t
ORIGIN      
        1 ccaaaccagt acaaccacag caggttgaat agtatgtgca agaaattttc gtggaaaaac
       61 atgtaggtta gcagcgtcca cggtcgagaa agtagctggt gtaaacctgc gggaacggca
      121 agccagtggg cgtagctgct gcccggctgt gcgccaccta tgaacatgat taccctaaca
      181 aggctataga tcaggaagat tgccagatta atgtagatca gctgggtaag gctgctgcca
      241 aacctgaagg aatcttttat ttctttcgaa atgctcatgt tgtgtagctg gtagtgtaaa
      301 tttataagtt ttggtcgaaa tatccaagca gcaacttttg agagtggtaa aagctgttag
      361 catgttaggg ttgcaacaat ggcatatttg ctcataagca aatatttcca atttactaaa
      421 ctataagggt agcccattgg ttgttttcta caatttttca ataactagta gaaaacattt
      481 tttctcgatt gccagtattt tatcaggaaa aagccaaaaa tcattccacc aagatgggca
      541 aagtgggcaa tgttacttcc ggcttgcgta agtccaagga acagctcaag tccgccgtaa
      601 aggataacca tccatttggc tttaatgggg atggggggaa acagaatcat cagaacggtg
      661 ttggggaaaa gcattccgaa tgcaagcagc actccataaa cagcacccga tgcgccaacc
      721 gtaggaatat tcagctgcag ggaaagaagc tgttggctta gctgctgggc ctgatcaatc
      781 ataatcgggt tgcttggatt ctggaaccac ttatctagaa agtcggagta aatactgttg
      841 tagaactcgg gaaagtgctt ggtaacaaac gcggcgaagg tatcgggcga aagcgtattg
      901 agcatggcaa tagaatctgc atgaatagag gagaattgca ggtagttaac aagcgagtga
      961 acggcagcag ctccaagtcc ggtaaccatg tagtatagca aaaacctttt accgccccag
     1021 acctgttcca gtattctacc gaacatccac agggcaaaca tgttgaaaaa caggtgggta
     1081 aaaccgccgt gcatgaacat gtgggtaacc acctggtagg gtttaaagtg aggcgacccc
     1141 ggtgtgtaaa gccccagcat ggcattcaaa tcgaccccgt aacgttgcaa catccaggta
     1201 accagccaaa ttacagtatt aatcaaaata agatttttta ctaccggggg tatgctgctt
     1261 agaaatccgc ttctacttaa actcattgtc tttaaaaatt taaagggtta aaacccgtta
     1321 caagttaaac atgaaataat gattaaggtt tttgagctac ttaagccatt tttcaaactc
     1381 gctcatttca ataagagcaa tagttttttt gccgtcggcg gtatagtttg gttccctgca
     1441 ggcaaagagc tggtcaacca cctcctgcat ttccgattcc gagagccgct tgccgtagcc
     1501 cactgcagca gctttagcca tggatagagc aattttctcc tttgcgctgt cgaagggtag
     1561 ggcttcattt tcgttaataa ttgccagcag gttttcgagg aaaagtgcgg ggtcggttat
     1621 ccgcgtgtta ccgggtaagc tgttaatgct tatggtgtta tggtttaaat tgctaatatc
     1681 cagacccagg cgtaggaaat tttcctggtt agtgatgata gtctcaaagt cttgcgggtt
     1741 tagctcgatg gtttgagggt aaagctcgcg ctgggctaca tttttttcaa ggttaagatt
     1801 tttgtagaat tgctcgtaga ggatacgctc gtgtgctctt ttctgatcaa ccaccagcat
     1861 ccccgattta actgcagtga tgatgtactt tcccttaatt tgaaagaacc tgccagtatt
     1921 ttcggcaact gagtcgtcaa aaccctttga ggcgaatgtt tggacggctg tttgtttggg
     1981 ctcaaagtta aagttggggt tgacgtcctg ataaagttgc tcccatcctt taaccggttg
     2041 ctgcggggta aagttgtgtt cccttgtcct ctgggcaaac ctggctcctt catcctcaaa
     2101 cgggttaaaa ttagggttaa gctcaatttc agggagttca accggtgaat ctttggggaa
     2161 atggggaatg ctgaacccag ggtcggagct gaagtcaatg gagggggcca cagcaaattt
     2221 ccccagtgcc tctcttacag ctgcatggac aatctgccat actgcaccat catcctcaaa
     2281 cttaacctcc gttttggtag ggtgaatgtt tacatcaata gaacccgggt caaccctgaa
     2341 gtaaataaag taggttggaa tgctgtttgc tggtagcagg cggttgtaag catttaccac
     2401 tgccttgtgg aggaagggac ttttcatgaa cctcccgttg acaaagaaaa actgttcacc
     2461 cgggctcttc cgggcacttt ctggcttacc aatgtaaccg ctaattgtaa caatagaggt
     2521 ttccgcaacg caatcaacca gctgcgagct gatggacttt ccgaaaaggt tcacaatgcg
     2581 ctgcttcagg tttgatgggg caagcttgta aatctccgag ccattatgcg ataggctcat
     2641 ggaaatctcc ggaaaggcaa gggcaacatg ctgaaattca gatatgatat gcttcagctc
     2701 caccgagttg cttttaagga acttccggcg tgccggtacg ttgtagaaaa ggtttttaac
     2761 agtaagagtc gaaccaacag ggcagctcac cggttgttgg gcaacgaatt ccgatccgcc
     2821 tacctgaacc attgagccaa cctcgtcgga caacctgcgt gtacggagtt caacttcagc
     2881 tactgccgca attgaagcca gggcttcgcc cctgaagccc attgttcgaa tgctgtataa
     2941 atcggccgct tccttaattt ttgaagtggc atgacgctcg aagcaaaggc gcgcatcggt
     3001 ttcggacatc ccaataccat cgtccaccac ctgaatcgaa gtttttccac catcctttac
     3061 tatgagctga atggccttgc accccgcatc aatggagttc tccaacaact ccttaactac
     3121 cgaagcggga cgttgaacca cctcgcctgc agcaatctgg ttggcaattg aatcgggtaa
     3181 tagatgtatg atgtctgcca tctgggaaat taccattaaa aaatgaggtg ggctaccagt
     3241 aaaagaattg tcaaaacttc cagaatattc ctggttggta aatagctgtc ggggtgtctt
     3301 atccacatcg ctcccatgcg ctcgggggta aatgccgagt ttgccctatc aaccgaacct
     3361 cgaactattc ctttgtgggt tttaacccag aaaatggtgt ctctgtgctc ttgtgctcca
     3421 aaaaagaaat tatttaccat gccgacctgc cagcctaatc aaacctccaa aattactcat
     3481 tttaatttat tgttttgaga cggttagata ttcacttggg atttgaaagg aaactcaacg
     3541 agcaaaaata gcagatagtt gtttactagc ccgaaatctt taaattcacg aagtgtaatt
     3601 ttggttaaaa ttttgtagct tacacgtagt ttaaaaaatt tcgatattat gaagcccgct
     3661 ggtgctgttg agctgattat cttgactgct gcatccatgc ttaacggttg cacaaagaat
     3721 gttgagccgt ttcctacaag cgctactctt agcccatcgc tttcggttcc gcttggggaa
     3781 ggcggtgtgt cgcttactaa aacccttcaa actcttggaa taccagtggt caatctttca
     3841 gaagatgttc cacaatgggc tacctatgga tttgtttacg ttgccgatac cattcccctt
     3901 aacctgaccg aagtatacaa ccgtggcgat tctattacat acctgatggt tagaacaaat
     3961 atctggaatc aatttccgtt gggtggcagg gcacaggtgt ttttccttga tgctaacaac
     4021 caagttatcg atcagcttta tgaggatatg gtgagtgttg gcccagctga gcatggaagc
     4081 catggcgagg ttgtaaacac tgctttcgaa tcgaacgaaa ccagcttcaa taccagcaga
     4141 atcagcttat taagcgcagc ccaaaaggtg gtaatatatg ccggtttgga aattacagat
     4201 gaaggcgttt ccgaatcgaa tattacctac ttcgaccagt accagcttag ggttcaggtc
     4261 gctttacagg ttgattttat catgaaccta aactagcaaa ttatcgtggc attgcggcga
     4321 atagtcaacc tgctgatttt tttctctgcc tacagcacca ttgtttgtgc ccagcaggac
     4381 aacacgctgt tcctgcttca taatattccc caatcaaact tcaccaaccc ggcagtgcag
     4441 attcagtgcc cggtgtatgt gggagttccc ctgctgtcct ctgttcattt caacgtaaat
     4501 tccacgggat tctcgtatga cgatgttacc aatgaatcgg ggattacaaa cctcaacgcc
     4561 ctggttgccg ggatgcaccg gtgggatttt gtttcggagg aggtgcactt cactccagtt
     4621 tcgtttggtt ttatgtacga caggcaccag tacattagct ttgcatggac ggagcgggtt
     4681 gaaacaaagg tgttcctccc aaaaaagttg ctttcccttt cggttgatgg aaatacccag
     4741 tacgttggcg atgagctgaa aaccggcaac ccaggggcga atgccgtgta ctaccgcgaa
     4801 ttttcggttg gttactccaa aaaggtaaat tccgggttga ttctgggtgt tcatgggaaa
     4861 ttattgtttg gcctggccgg tgcgtttacc cgtcgtaggc ctgttaatgt tgatgttgat
     4921 gccattaccc ataacattga tgcccaatgg aatccgaagg tggatgttgc atacccgcta
     4981 ggtgtggcaa ccgatgcaag tggtaacgtt agcaatgtta ccgttggcgg tttttcaccc
     5041 ttgaattttt tcctgaattt tggaaatcag ggaattgctg ctgatttggg ttttatatgg
     5101 cagcatgatg atgttacatg gagcggtagc ttgctggatt tggggtttat ttggtggcac
     5161 aagcagacca cccggttcga gaattcaggt cattttgttt atcgcggcgc tacccctgcc
     5221 gatttggaca atcccgatga ctacatcaac cagctgggcg attccctaaa caaccaaatc
     5281 caggtggaat actcccgcaa aggattcatt acactgctga acccaaaaat ttaccttggt
     5341 gcaagctacc cggtatcgga aaggtttaat gttggagccc atgttcgtgc agagtggtac
     5401 cccggtcgcc ctgttatggg tattacctta tccggaatgt tttttacgaa aaagggtggt
     5461 tcggcagccc taagctactc ggtgatgaat ggttcgttca tcaacgttgg cgctggcctc
     5521 ggttggggtg gccaaacttt tcaattctac gtggtgtccg acaaccttat ggctgcgctc
     5581 tatcccgaaa aggcccgaaa cgccaacctt aggtttggtt ttaacctctt tttcggctgc
     5641 atcgaaaaaa agaagaaggt ggaggtgccc catggcaatg gatgcggctg ctactggagc
     5701 tgggatgata aaaccaggcg aaaacttggt agaaattagc tgctgtccct cttatttggg
     5761 gttgagcgaa ccattttcgt cccggttaca agtaaattga aatttttgcg ggttaatacg
     5821 atactgcaaa agatattttg cccattgctt tcttaaaaac tatttggact gttaaagctg
     5881 accggctaaa aattgtaggg ttctgccttt tgtcttttcc acattttcat tatttttact
     5941 tcgttgaaat ttttaatata acaagtttta aaattttggt tgaaaatcgt aataagctgc
     6001 tgatgaaaaa aatatgccga atatttgcaa ttgcagtatg gatggcccct ttaggtctta
     6061 tggcacagcc ccctgaagta ggtcaggttg ccccggatat cctgctttcc agcccaactg
     6121 gagattccat aagactttcg tcgctaaggg gcaagcttgt gctggttgat ttctgggcat
     6181 cctggtgttc accctgtcgc agggaaaacc ccaacctggt gcaaacctac aacaagttta
     6241 aagacgcctg ctttgagaat ggtaatggat ttaccattta tagcgtttcg ctcgaccaga
     6301 agcaggatgc atggttgaaa gccatagccg acgatggcct ggtttggccc taccaggtta
     6361 gcgatcttaa aggctggcgt agcgaggccg caaagcaata tggcgtgagg gcaattccca
     6421 tgaactacct tatcaacgcc aacggcgttg tggtggctac aaacctcagg ggcgacgagc
     6481 tcgacagcac cttgcggaag ctgcgtaagt ggaggatttt ctccaatgag tgtatttgat
     6541 aatcacatca actagctaac ttttatcgac atgaaggttg gtcatattct gtggaaaaca
     6601 gctaaaataa ttttaattac cctggggtcg ttaatcctgt tggtagttat actacttgca
     6661 gcagctaagc tgtcggaaaa taagatagtt gggctggtgc tcaacaaggt aagcgtggag
     6721 cttaaggcgc cattctccgt cagggaggtt aacctactcc ccttacggaa ttttccaaac
     6781 ctgactgttg aacttcagga ctttagcgtt ggtaaatcaa ccgattcgct tcaatcaggg
     6841 cagagcagca acccttttga tacacttttt agcctgcata agctctacgt tgccgtgaaa
     6901 gctaaaccac ttctgagcag caaggttgta gttgaccagg tggagattga cggggcaacc
     6961 ctgaactatc atgttgactc caccggtgca agcaacttcg atttcctgat gactaccacc
     7021 gataccaccc aagtacctga
//
LOCUS       DAOOQX010000016         6831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_13621_length_6831_cov_3.105815, whole genome shotgun sequence.
ACCESSION   DAOOQX010000016 DAOOQX010000000
VERSION     DAOOQX010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13621_length_6831_cov_3.105815"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLX53_00585"
     CDS             complement(<1..587)
                     /locus_tag="PLX53_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOY71607.1"
                     /translation="MYPDYHTHTNLSDGRDTHEEMFASAQIRDVSEIGFSDHICVKPV
                     SWAMDVRNIPAMVEAVQMLKQKEGVVRVKQGAEVDFIPGRETETRELLSFIPLDYCIG
                     SVHFIDDWNFDTDILPYDQISINAFYKTYFKLVCQSAKSGLFDIIGHCDLAKKFAYYP
                     TISLDRIFEETARVFSDYGVTVELNTSGKNKPCAE"
     gene            848..1645
                     /locus_tag="PLX53_00590"
     CDS             848..1645
                     /locus_tag="PLX53_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HOY71608.1"
                     /translation="MRTNLLGIIAISGVISLMVSCQPQAEKQEYNQDHMLYSTIWYQH
                     SPEMQALYYQAFNIAGERVRQQSSHKTSKPKAVVVDIDETMLDNSPFQAQEILDNREY
                     STEFWKEWTSLARAKALPGAVEFSKLCDSLKVTLFYITNRSADEAASTLKNLDSLGFA
                     FAKPEYLLPKENESSKKQRRERIAEQYDIILLVGDNLNDFSEIFENRGDDWGSGLVGK
                     NKSDFGSRFIILPNPMYGDWEKNIYHTRGLSPQQKDSLRRSLLNGFR"
     gene            complement(1872..1946)
                     /locus_tag="PLX53_00595"
     tRNA            complement(1872..1946)
                     /locus_tag="PLX53_00595"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1911..1913),aa:Arg,seq:cct)
     gene            2203..3480
                     /locus_tag="PLX53_00600"
     CDS             2203..3480
                     /locus_tag="PLX53_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOY71609.1"
                     /translation="MSKELMHETLNTLGLEEDDEPPGSGQLSPNYGLITATSHQHKSP
                     SCNWPFPISKRSLEFLIKYFPNATVEDWMSWKWQLKNSFTTSEKLKCFLDLSREECEA
                     IDHPKHSLPLRITPYYASLLNGKNSQDPLRRTMIPTVNELTTSAGESCDPLNEGHSSP
                     VPNLVHRYPDRVLFLSTGFCSAYCRYCTRSHMVAKDKCHVGVNAWLPAINYIREHTEV
                     RDVLISGGDPLTMSDAHIEYLLSQLRSIPHVEMIRIGTKVPVVLPHRITPKLIKILRK
                     FHPLYISIHFTHPDELTPETAEACRRLADAGIPLGSQTVLLKGINDNVDTMKSLFQGL
                     LKIRVRPYYLYQCDPIPGSSHFRTSVEKGLEIIKGLRGWTTGYAIPHFVIDAPGGGGK
                     IPLLPEYYQGRNEEGYVVLKNYEGKTFYYPDLH"
     gene            3655..4638
                     /locus_tag="PLX53_00605"
     CDS             3655..4638
                     /locus_tag="PLX53_00605"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOY71610.1"
                     /translation="MTVGLTYDLKSDYLKNGYSHEEVAEFDSDETIDSIESTLQSLGY
                     FTERVGNIINLTHALANGKRWDMVFNIAEGLHGIGREAQVPAILDAFQIPYVFSDPLV
                     LALTLHKGQAKRVVRDCGVPTADFAVITSLSEVEKIALPFPLFVKPAAEGTGKGINGQ
                     SKINSKEVLYATTARLLEEYRQPILVETYLPGREFTVGIVGTGDKARCLGVMEIVISN
                     RAKDGTYSLHTKENWKGIVDYYMATGEIESLCAETALMAWNCLGCRDGGRVDLKMDAS
                     GKPNFIEVNPLAGLNPFHSDFPMLAQKNGIDFTMLIQLIMDSAMERFNHTA"
     gene            4635..5621
                     /locus_tag="PLX53_00610"
     CDS             4635..5621
                     /locus_tag="PLX53_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOY71611.1"
                     /translation="MNRQTIHAVILYNELSESAGPDERDVLDQVALVAEMLNKLGIES
                     QRAEFSLNLQKVRDELSRLKPDFVFNLVESVNNRGNLIFLAPSLLNSMGIPFTGGSLE
                     TIFLTSSKTLTKQLMQDAGIPTPFWYQGNGSFIPDRDKRYIVKPIWEDGSLGLDEDSI
                     FEGSNPKLAEILNSIDYNTHFIEEFVDGREFNISILAGQNGPQLLPPAEIIFRNYAPD
                     KPHVVGYSAKWIEDSFEYINTNRTFDLSPNDQPLLSTMGDICLHCWKTFRVNGYARVD
                     FRVDNAGNPWVLEINVNPCISRDSGFYAACMQAGIPFDEAVRSIIADIPFQI"
     gene            5638..6120
                     /locus_tag="PLX53_00615"
     CDS             5638..6120
                     /locus_tag="PLX53_00615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY71612.1"
                     /translation="MDNVTFRSTPTPADKAGIREILSSSGFFHDFEIDVAIELVEEAL
                     AKGTESGYYFVFADHMGTTISYACFGPIPCTRHSFDLYWIGAHQDYRGKGLGKQVLQR
                     VENEIKQLNGRKIYIETSSKPMYEPTRQFYLSNAYENEAILKNFYDEGDDKVVYSKSL
                     "
     gene            6505..>6831
                     /locus_tag="PLX53_00620"
     CDS             6505..>6831
                     /locus_tag="PLX53_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOY71613.1"
                     /translation="MQEKFRVDVIDQKILSYLVKNARIPFLQIARECGISGAAIHQRV
                     KKMEDAGIIAGSRMIVKPKALGFDVCAFVGVQLVSTNLYSGVIEALKLMPEVVECHFI
                     TGEYALL"
BASE COUNT         1988 a         1585 c         1489 g         1769 t
ORIGIN      
        1 aactcggcac agggcttgtt tttcccgctg gtgttcagct caacggttac cccgtaatcc
       61 gaaaagactc ttgccgtttc ctcaaagatt cggtcaaggc tgatggtggg gtaataggca
      121 aatttcttgg ccaggtcgca gtggccaata atatcgaaaa gtcccgattt tgccgactgg
      181 caaaccagct tgaagtaggt tttataaaaa gcgtttatgc tgatctgatc gtagggcaga
      241 atatcggtat cgaaattcca atcgtctata aaatgaacag aaccaatgca gtagtcaagc
      301 ggaataaagc tgagcagctc ccgagtttcg gtttcccgcc cgggtatgaa atcgacctct
      361 gcaccctgtt taaccctaac cactccttct ttttgcttaa gcatttgaac cgcttcaacc
      421 atagcaggaa tatttctgac atccattgcc cagcttacgg gttttacgca gatgtgatcg
      481 gaaaatccaa tttcagaaac atctctaatc tgggccgaag cgaacatttc ctcgtgagtg
      541 tcgcgcccat cggataggtt ggtgtgagtg tggtaatcgg gatacatgat ataaatttca
      601 acaattatgg gtaaaaaatg ttgccaacta ccaaaagtat gcaatttttt accaaaattt
      661 ttccatgcaa aatccactac tggtaaaacg cttaacttta caggctgcaa ctgctctaaa
      721 gccagctgtg attcctttgc aaatatgtta aagtttgaac gattagatgg aaagcccatt
      781 tagttagcat aaccttaatt attaagtagt tcgctaccgt ataaccatta aattatcaag
      841 gtaaaccatg agaacaaatc tactagggat aatcgctatt tcaggcgtaa tatccttgat
      901 ggtgtcgtgc caaccccagg ctgaaaagca ggagtacaac caggatcaca tgctgtactc
      961 cacaatttgg taccagcatt cacccgaaat gcaggcgcta tactaccagg ctttcaacat
     1021 agccggcgaa cgtgttagac agcaatcgtc gcacaagacc tcaaagccaa aagcagtggt
     1081 ggttgatatt gatgaaacaa tgctggacaa cagcccattt caggcacagg aaatattaga
     1141 caatagggaa tactccactg aattctggaa ggaatggaca tcccttgcac gggcaaaagc
     1201 ccttccgggt gccgttgagt tcagcaaact ttgcgattcg ctcaaagtga cccttttcta
     1261 cataaccaac cgctctgctg acgaggcagc atcaacccta aaaaatctag actcgttagg
     1321 ttttgcattt gccaaacccg agtaccttct gccaaaggag aacgaaagca gtaagaagca
     1381 gcgccgtgaa aggattgcgg aacagtacga tattatactg ctggttggcg acaacctcaa
     1441 cgatttctcc gaaatatttg agaatcgcgg cgacgattgg gggtctgggt tggtaggcaa
     1501 gaataaaagt gattttggca gccggttcat catccttcca aacccaatgt atggcgattg
     1561 ggaaaaaaac atttaccata caagggggct ttctcctcag caaaaggaca gcttgcgtcg
     1621 ctcgctttta aatggattca ggtaacattc aacctttgtt tctacccaac tagctagtag
     1681 gatatttgca tcattctgtg tggctgctgg agtgctgtat ataaaattag ccatgctgca
     1741 aattctttag catgaaaatg ttgctgttag tgtgtaatgc gactaacaac agtatagtct
     1801 ttaagtttgt gtctccacaa tccggaggta ataaaaaaag cctttatgag cttaaaggct
     1861 tgttgttagg ttggcggcct taccaggact cgaacctgga tttaaagttt aggaaacttc
     1921 cgttctatcc cttgaactat aaggccataa ccagctgcaa agataggaat atttttatat
     1981 gttaaccaat aatccaaggg gttgcatctt tacgcctgtt gataactcat gcaaactgtt
     2041 aattactatc gccttcccca tgaataaaaa tgctgatagc aacttaattt cgtcgaaatt
     2101 tttaaaaaca ccggcattaa tgatttttta gctaaaaaaa ttaagaagcc tgaacaaggt
     2161 attgaataaa taacaacatt tgcgacgcta aattttaaag tcatgagcaa agaactaatg
     2221 catgaaacgc ttaacacgct aggcttagaa gaagatgatg aacctccggg ttcggggcag
     2281 ctaagcccaa attacggttt aattaccgca acttcccatc aacacaaatc accctcctgc
     2341 aattggccat ttccaatcag caaacgttcg ctggaattct tgataaagta cttccccaac
     2401 gccacggtgg aagactggat gagctggaaa tggcagctga aaaacagctt cacaacttcc
     2461 gaaaaactca aatgcttcct tgatctttcc agggaggagt gcgaggcaat tgaccacccc
     2521 aaacattcgc tgccattacg aataacaccc tactacgcca gcctgctgaa tgggaaaaac
     2581 tcccaggacc cgctacgtcg gaccatgata cccaccgtta acgagcttac cacctcggca
     2641 ggtgagtcgt gcgacccatt aaacgaggga cattccagcc ccgttccaaa cctggtacat
     2701 cgctacccgg acagggtgct gtttctttca accggattct gctcggccta ctgtcgttac
     2761 tgcacccgtt cgcacatggt ggccaaggat aagtgccatg ttggggtcaa cgcctggcta
     2821 ccagcaatta actatataag ggagcacacc gaggttaggg acgtcctcat ctcgggtggc
     2881 gacccgctta ccatgtccga tgcacacatc gagtacctcc tttcccagct tagaagcatt
     2941 ccacatgttg aaatgatacg gattggcacc aaggttccgg tcgttcttcc ccatagaatt
     3001 actcctaagc tcataaaaat tctccggaaa tttcatcccc tctacatcag catccatttt
     3061 acccatcccg atgagcttac cccggaaaca gccgaggcat gcaggcggct tgccgatgct
     3121 ggtattccgc ttggtagcca aactgttctt cttaaaggta ttaacgacaa tgtggacacc
     3181 atgaagtccc tatttcaagg actgctaaaa attagggtcc gaccttacta cctttaccag
     3241 tgcgatccca tacccggctc ttcccacttt agaacatctg ttgaaaaggg ccttgagata
     3301 attaaaggac ttaggggctg gaccaccgga tacgccattc cccactttgt tattgacgca
     3361 cctggtggcg gtggaaaaat tccccttctg cctgaatact accaggggag aaatgaggag
     3421 ggatacgttg tgcttaaaaa ctacgagggg aaaacctttt actaccctga tttacattaa
     3481 aacaatggca attgtatagt tcaaccacct gtccctttgc tttcacttac ttcggacagg
     3541 tggtttttac cataacgcta tttactgcac acctttttca aaaaaataaa cttgatgtgc
     3601 tcaaatggct ttcccaagag tttttaatgc tcacgctgat aaacacaaaa atagatgaca
     3661 gttggcttaa catacgacct gaaaagcgac tacctgaaaa atggctactc acatgaggaa
     3721 gtggccgagt ttgactctga cgaaacaatt gacagcattg agagtacact gcagtctctt
     3781 ggctacttca ccgagcgggt tggaaacatc atcaacctca cccatgcgct ggccaacggc
     3841 aaacgttggg acatggtttt taatattgcc gaagggctgc acggaattgg aagagaagca
     3901 caggttcctg ccattctgga tgcgtttcaa attccctacg tgttttccga ccccctggtg
     3961 ctggcactta ccctacacaa ggggcaggca aagcgtgtgg ttcgcgactg cggcgtgcct
     4021 acagccgatt tcgccgttat tacaagccta agcgaggttg agaaaatagc tctgcccttc
     4081 cccctgtttg taaagccagc tgcagaggga acaggaaaag ggattaatgg ccagtcgaag
     4141 ataaactcaa aagaagtgct ctatgcaacc acagcacggt tgctcgaaga ataccgtcaa
     4201 cctattcttg tcgagaccta ccttcccggg cgtgaattca ccgttgggat agttggtaca
     4261 ggcgataagg cacgctgcct aggggttatg gagatagtga tttccaacag agcaaaagat
     4321 ggtacctact ccctacacac caaggagaat tggaagggca ttgtggacta ctacatggca
     4381 accggcgaaa tagaatcgct ttgtgccgaa actgctctaa tggcatggaa ttgcctgggt
     4441 tgtcgcgatg gtggaagggt tgacttaaaa atggatgctt cgggcaaacc caacttcatt
     4501 gaggttaatc ctcttgcagg cctgaatcca ttccactctg atttccccat gctcgctcaa
     4561 aaaaatggca ttgattttac catgcttatt cagctgataa tggattcggc aatggaacgg
     4621 tttaatcata cggcatgaac aggcaaacaa tacatgcggt aatcctatac aacgagcttt
     4681 cggagagcgc cggtcccgac gaaagggatg ttctggacca ggttgccctt gttgccgaga
     4741 tgctcaacaa actgggtatt gagagccagc gcgcggaatt ttcccttaac cttcaaaagg
     4801 ttcgtgatga gctcagcagg ctaaagcccg actttgtttt caacctggtt gaatcggtga
     4861 acaacagggg aaacctgatt ttccttgccc cttccctgct taactctatg ggaattccat
     4921 tcaccggcgg ctcgctcgaa accatattcc tcacatcatc caaaacgcta accaagcagc
     4981 tgatgcagga tgcaggcatc ccaacgccct tttggtacca gggaaacggt tcatttattc
     5041 ccgatcggga taaacgttac attgtaaaac caatctggga ggatggatcg ctgggcctcg
     5101 atgaggattc aatttttgag ggaagcaacc cgaaactagc cgaaatttta aatagcattg
     5161 actacaacac ccattttata gaggagtttg tggacggccg cgagtttaac atatctattt
     5221 tggccggaca aaacggtcca caactattac cgccagccga aatcatattt cgtaactatg
     5281 ctcccgacaa gccgcatgtt gttggctact ccgccaaatg gattgaggat agctttgagt
     5341 atatcaacac aaacagaact tttgacctgt ctccaaatga ccaaccgttg ctcagcacca
     5401 tgggtgatat ttgccttcac tgctggaaaa cctttagggt gaatggctac gccagggtag
     5461 attttcgggt tgataacgca ggaaacccgt gggtgctgga aatcaacgtc aacccttgta
     5521 tttcgagaga tagcggattc tatgcggctt gtatgcaggc aggaattccg tttgatgaag
     5581 cggtaaggag cattatcgcc gatattccct ttcaaattta acataacgtt cagccttatg
     5641 gacaatgtaa cttttcgctc caccccaacg cccgccgaca aagccggaat cagagaaata
     5701 ctatcctcat ctggattttt tcacgatttt gaaattgatg tagccataga actggtggag
     5761 gaggctttgg caaagggaac ggaaagcggt tactactttg tttttgccga ccacatgggc
     5821 actacgatat cctacgcctg ttttggtcca attccttgca cacgccacag cttcgatctg
     5881 tactggattg gagcccacca ggattacagg gggaaaggcc ttggcaaaca ggtactgcaa
     5941 agggtagaaa atgagattaa acagctgaat gggcgaaaaa tatacattga aacctcatca
     6001 aaaccaatgt acgagcccac ccggcaattt taccttagca atgcctacga gaacgaggca
     6061 atacttaaaa acttttatga cgaaggcgac gataaggttg tatattcaaa aagcctttaa
     6121 acataaacca gcatattcta agcccttttt gactttttat ctttgcaaag caaaccagtc
     6181 aggaaatgaa tcagagagat gcatctacta aaaatcaacc ttcatttttt ctcaaacccg
     6241 acatggagaa aggagttgcc atgtcatcaa ttgtccttca tgtcatcatt taaaatagaa
     6301 gcaacttgtt ttaatactgc caagtaccgt tcgaaattaa gcaccgtttt ttttgcaaca
     6361 caacaaactc cctgaatatg taagcgtttt aggaaatcat tgcaaaaaaa ttaatcagat
     6421 aaggttaatt tttataattt attttgtctt tttaacaata catttttaat tttatcaccc
     6481 aaaacgtatc aattcaaaac aaccatgcag gaaaaattta gggtagatgt aatcgatcag
     6541 aagatacttt cgtacctggt aaaaaacgcc aggataccat ttcttcagat tgcaagggag
     6601 tgtgggatat cgggagcagc catacatcag cgagtaaaaa agatggaaga tgccggaata
     6661 attgccggat cgcgaatgat tgttaagccc aaagccttag ggtttgacgt atgtgcattt
     6721 gtaggcgtgc agcttgtttc caccaacctg tacagcggcg tcattgaagc cctgaaacta
     6781 atgcccgagg tggtagaatg ccattttata acaggagagt atgctttgct g
//
LOCUS       DAOOQX010000017         6696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_13979_length_6696_cov_4.223611, whole genome shotgun sequence.
ACCESSION   DAOOQX010000017 DAOOQX010000000
VERSION     DAOOQX010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13979_length_6696_cov_4.223611"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..145
                     /locus_tag="PLX53_00625"
     tRNA            69..145
                     /locus_tag="PLX53_00625"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:103..105,aa:Pro,seq:tgg)
     gene            404..1501
                     /gene="meaB"
                     /locus_tag="PLX53_00630"
     CDS             404..1501
                     /gene="meaB"
                     /locus_tag="PLX53_00630"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOY71614.1"
                     /translation="MKQRKNKDDESALFVAPGIDQPPVVNEQAVQKFRQQKRRQPSID
                     EFVGGILARNNTILSQAITLVESSLPRHYELAQQLIERCLPYSGNSIRVGITGIPGVG
                     KSTFIESLGTMLTSQGHRLAVLAIDPSSTRSKGSILGDKTRMESLSADANAFIRPSPS
                     AGSLGGVARKTRETIILCEAAGFDTVFIETVGVGQSEVSVHSMVDFFLLLMLAGAGDE
                     LQGIKRGIMEMADLIAITKADGANISRSKLAQAEFQNALHLFPMPESGWVPKVVTCSA
                     LNKVGIDEIWREMVAYADFTRKNGYFVSRRNRQAKYWMHETINEALKNKFYADRLIHE
                     QVRELEKEVVANRLSPFAAAQLLLDRYFSLK"
     gene            1640..2167
                     /locus_tag="PLX53_00635"
     CDS             1640..2167
                     /locus_tag="PLX53_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018451909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOY71615.1"
                     /translation="MDKKQFLSTILNRKSVRSFTGKPLGQDDMELLLRAGMAAPSAHN
                     LQPWKFIAIVDRQILNSLAEKLPYAKMLFEAPAAIVVCGDLTKTGSSPKDYWVQDCSA
                     ASENILLAAEALGLGAVWTGVYPRAERVETVQGILNLPSEIVPLNVIPVGTPKGEHQA
                     KDKFKSENIQWNRWP"
     gene            complement(2164..3540)
                     /locus_tag="PLX53_00640"
     CDS             complement(2164..3540)
                     /locus_tag="PLX53_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOY71616.1"
                     /translation="MVKTAKPKKEKKESRIQRLKKKFEQIYSFITHDIWVLQVDELSP
                     KLKAFYKYLRVGLLALRGFNEKKVSVKASALTYYTLMSIVPIFAMVFGIAKGFGLDKF
                     LEQQIRSSLEGQEEVMNKLITFSSSLLERTGGGIIAGIGVVLLFWSVVMVLSSIENAF
                     NDIWQIEKPRTYVRKFTDYLSIMVIAPILLLAASGIQIYLSTQVSTIAEKSQIIHIIS
                     PVLLSGFKLVPYLLIWILFSFLFITLPNTKVSIVSGIIAGILSGTAFLIVQWAYVYLQ
                     IGVSKYNAIYGSFAALPLFLVWVHTSWLIVLFGAEVSFAYQNVEMYEFEKETDQISHF
                     NRKVLSILILNTIIKRFNNGEEPLTSLDLSKQLKIPQRLMRNLLTQMVECGFLNEVVT
                     PIAKNYAYQPARHIDHLTLAYIEDKLDNHGLKIEPNSDDYKHIAEVYRNFNEKYKALS
                     KETLIGSL"
     gene            complement(3655..4779)
                     /gene="dprA"
                     /locus_tag="PLX53_00645"
     CDS             complement(3655..4779)
                     /gene="dprA"
                     /locus_tag="PLX53_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOY71617.1"
                     /translation="MSADPELVYKIGIELIPKVGSISAKKLIAYCGSAEAVFKQKKGS
                     LLKIPGIGEATAAEIVAQTVLSRAENELLFIEKFGIKVLYFTDAAYPYRLKQCEDGPV
                     VLYVKSNINIDFNTDKVLSIVGTRRATEYGKAMCEDLVSGLAEKGYRPIIISGLAYGI
                     DVTAHRAALKRNLPTVAVLGHGLNTIYPQSHRSVAKAILNQGALVSDFTSDAVFDRNN
                     FLRRNRIIAGMADATLVVESALEGGALVTADIANSYNRDVFAVPGNVNSVYSQGCNQL
                     IKQNKAALVESADDLEFLMGWTTSKVNSSRQYSINFDKFSADEQKILNFLKEKGEETV
                     DVIAMDTGLQVSKVLSLLLNLEFAGVVRSMPGKVFALAIG"
     gene            complement(4776..5258)
                     /locus_tag="PLX53_00650"
     CDS             complement(4776..5258)
                     /locus_tag="PLX53_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71618.1"
                     /translation="MKFDNDDTVTGIHSAKLIMAIIAIILIVLLIATGLSDFLLRTTG
                     IDRDWAIAIILLLYILFSGYFYLRRASYFSYNDEGKKIIIRYFLLRPFKAPKISIEVD
                     KSEFYRFTIEKKFLRRELSIFVRNGNKVSRYPAISISSLTGKQLEQLVAALNSYVKGK
                     "
     gene            complement(5430..6578)
                     /locus_tag="PLX53_00655"
     CDS             complement(5430..6578)
                     /locus_tag="PLX53_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOY71619.1"
                     /translation="MIALDNTGNAFQYLSNKELKRACRLFTLLSKLGGMNRSGKANSW
                     ASIPPVNFLLKNRIFDFFSGGLTLESCESVGDELWGYGVYSSPAPLVECNDKQLVFDE
                     YLRAIRIARDFPNHAFTLVKLSTLVEPRILRLVSQNAMLNHADKGIYTEFANRVRLLC
                     NEAFSSGKSIIIDAEHVDYQPAIDSIASEMMMLHNGDRATVYQTFRFDMVETFERLVE
                     QLRIASKNGYILGAKLLRGTLTHREVGESEKILFQNTQNDINHSLNEAIKFLLNNLAS
                     ASFIFGTHNHDTILYILSLMENLGVSPGDPQVFFAHHLGLADHLSFNLALSGYNVVKT
                     IPYGPKNIVEKGIVSSIIQHYSASHLREERHFLRKEVKRREKLVEGET"
BASE COUNT         1868 a         1503 c         1469 g         1856 t
ORIGIN      
        1 acggtttgct aaaccgtcgt acttcgcaag gggtaccggg ggttcgaatc cccccttctc
       61 cgcaccatcg gggtgtggcg cagttggtta gcgcacctgc tttgggagca gggggtcgtg
      121 cgttcgagtc gcaccacccc gaccaggagg cctaaacggc ctcctttttt ttgcttttat
      181 cgctgcatgt tccattgttt atgaaaacaa gcaactcgat gaatactagt tatttatgtg
      241 gatagccatt gccatacatc cagctggtgt cagagcctgg cttgcttcaa tctgataaaa
      301 aaacgatatt tgtcatgctc ttttagcgca acaccatgac tgctgttttt ttaacctgtt
      361 accattaacg gtaacagaac atgtgtcatt taagcaatgg ttcatgaaac aaaggaaaaa
      421 caaggatgat gaaagtgcgc tttttgtggc tccaggaatt gaccagccgc ctgttgtaaa
      481 cgagcaggcc gttcaaaagt ttaggcagca aaagcgccgg caaccgagta tagacgagtt
      541 tgttggcgga atacttgctc gcaacaacac catcctgagc caggcaatta cattagtcga
      601 gagttcacta ccacgacact acgagctggc ccagcagcta attgaacgtt gccttcccta
      661 ttcgggtaat tcgattcgtg ttggaattac aggaattcca ggggttggca aaagcacatt
      721 catcgaatcg cttggaacaa tgctaacatc gcagggacac aggttggctg ttcttgccat
      781 tgacccgagc agtacgcgaa gcaaggggag cattcttggc gataaaaccc ggatggaatc
      841 gctcagcgcc gatgccaatg ctttcattcg cccttcgcca tcagccggtt ctcttggagg
      901 cgtagcacga aagacaaggg aaaccattat cctttgcgag gctgccggat ttgatacggt
      961 gtttattgaa acggttggcg ttggtcaaag cgaggtttcg gttcactcca tggtggattt
     1021 cttcctgctg ctcatgcttg caggagcagg tgatgagctt cagggcatta aacgaggcat
     1081 aatggaaatg gccgacctga tagcaataac taaagccgat ggagcaaaca taagcaggtc
     1141 aaaattggcg caggctgaat ttcaaaatgc cctacaccta tttcccatgc ccgaatcggg
     1201 ttgggttcca aaagtggtta cctgctcggc tctgaataaa gttggaattg acgaaatttg
     1261 gcgtgagatg gtagcttatg ccgatttcac caggaaaaac ggctactttg ttagccgtag
     1321 aaaccgacag gcaaagtact ggatgcatga gactataaac gaagcgctta aaaacaagtt
     1381 ctacgccgac aggttaatac atgaacaagt aagagaatta gaaaaggagg tggttgccaa
     1441 caggttaagc ccatttgccg ccgcacaact ccttcttgac cggtatttca gccttaagta
     1501 gatactctgg caagcgggtc aacttgagct gctcaaacga tgaggaatgg ataggattat
     1561 tgcaaagttg attgacattg gctatccgta atagagtgag taaaaaaatt gcacaaccag
     1621 taaaacaaac tatgcgcaaa tggataagaa acagtttctc agcaccatac taaaccgaaa
     1681 gagtgttaga agttttacag gaaaaccgct cgggcaggat gatatggagc ttcttttacg
     1741 agccggtatg gctgctccct ctgcacataa cctgcaaccc tggaagttta tagcaatagt
     1801 tgacaggcag atattgaact cccttgccga aaaacttccc tacgccaaaa tgctttttga
     1861 agcccctgcc gctatagttg tatgtggcga tttaaccaaa acaggaagca gtcccaaaga
     1921 ttactgggtg caggattgtt ctgcggcaag cgaaaacatt cttcttgcag ctgaagcgct
     1981 aggcttgggt gctgtgtgga caggtgttta ccctcgtgca gaaagggttg aaaccgttca
     2041 gggaattctt aaccttcctt ccgaaattgt tccacttaat gttatccccg tcggaacccc
     2101 aaaaggagaa caccaggcta aggataagtt taaatcggaa aatattcagt ggaatcgctg
     2161 gccctaaagg ctcccaatca gggtttcctt tgaaagagct ttgtactttt cgttaaagtt
     2221 ccggtaaacc tctgctatat gcttgtagtc gtcgctgttc ggctcaattt ttagtccatg
     2281 gttgtccagc ttatcttcta tgtaggcaag tgttaggtga tcaatatgcc tggcaggctg
     2341 gtaggcgtag tttttggcta tgggcgttac cacttcgttc agaaagccgc actccaccat
     2401 ttgggtgaga agattcctca tcagcctctg aggaattttc aactgctttg aaaggtccag
     2461 gcttgtcaac ggctcttccc cattgttaaa ccttttgatg atggtattca gtattaaaat
     2521 ggaaagaact ttcctgttga agtggcttat ctggtcggtt tccttttcaa attcgtacat
     2581 ctcgacattc tggtaggcaa aagaaacctc ggcaccaaaa agtacaatta gccagctggt
     2641 atgcacccat accaggaaca ggggtagtgc agcaaaactc ccgtaaatgg cattatactt
     2701 tgatactcct atctgtaggt aaacataagc ccattgcaca ataagaaacg ctgtgcctga
     2761 aagtatgccg gcaattatcc ccgaaacaat cgagaccttt gtgttgggaa gcgtaataaa
     2821 caaaaagctg aaaagtatcc aaatcaacag gtagggtaca agcttaaacc ccgaaagtag
     2881 taccgggctt ataatgtgta tgatttggct tttttcggct attgtgctca cctgggtaga
     2941 aaggtaaatt tggattccgc tggcagcaag aagcagaatg ggcgctataa ccataatcga
     3001 aaggtaatcg gtaaattttc gcacgtaggt tcggggtttt tctatctgcc agatgtcgtt
     3061 aaaggcgttc tcgatgctcg atagcaccat caccactgac cagaaaagta acaccactcc
     3121 aatacccgca ataattccac cgcctgtgcg ctcaagcagc gaagacgaaa aggtaatgag
     3181 cttgttcata acctcctcct gtccctcaag gctgcttcta atttgctgct ccagaaactt
     3241 gtcaagccca aagcccttgg caattccaaa caccattgca aaaatgggga ctatggacat
     3301 aagggtgtag taggtaaggg ccgacgcttt aacgctaacc tttttctcgt tgaaaccacg
     3361 tagcgccagc agccccactc gaaggtactt gtagaaggcc ttaagcttag gcgaaagctc
     3421 atcaacctgt aaaacccaaa tatcatgggt gatgaatgag tatatttgct caaacttctt
     3481 tttaagccgc tggattctac tttccttttt ctctttcttc ggttttgcag tttttaccat
     3541 aaccttacaa aataaatttt ataaaggcgc ctagctccag gagcaggtgt taaggtactc
     3601 acgattttta ctgccaaata tttcggctcg aattattaca tcaaagtctc ataatcatcc
     3661 aattgctaag gcaaacactt ttcctggcat acttctaaca accccagcaa attcaagatt
     3721 cagcagcagc gataaaacct tagaaacctg caaacctgtg tccatagcaa ttacatcaac
     3781 cgtttcctca cctttttcct ttagaaagtt aagaattttt tgctcatctg cagaaaactt
     3841 atcaaagtta atagaatact ggcgtgatga atttaccttt gaggtggtcc agcccatcag
     3901 aaattctaaa tcgtcggcgg actcaaccag tgctgcctta ttttgcttaa tcagctgatt
     3961 acatccctgc gaatataccg agtttacatt accaggcact gcaaaaacat cacggttata
     4021 ggagttggca atatcggcag taaccagcgc accgccctcc agcgcgcttt caaccacaag
     4081 tgtggcatcg gccatgccag caataatccg attccgacgt aaaaagttat tcctgtcgaa
     4141 taccgcatcg gaggtgaaat cgctaaccag cgctccctgg tttagtatag ccttggccac
     4201 gcttcggtgc gattgcggat aaatggtgtt caggccatgc cccagcaccg caacagtagg
     4261 caggttgcgc tttagtgccg ccctgtgagc ggtaacgtca attccataag ccaacccact
     4321 tataattatt ggcctatacc ccttttctgc caatcccgag actaaatcct cacacatcgc
     4381 ctttccatat tctgtagccc gccgggttcc tacaatgctt agcactttat ctgtgttaaa
     4441 atcaatgtta atatttgatt ttacgtaaag aacaacagga ccatcctcac attgctttag
     4501 ccggtaaggg tatgccgcat cagtaaaata aagtactttg atgccgaatt tttcaataaa
     4561 cagtagctcg ttttcagctc gcgatagaac tgtttgggct acaatttcgg cggcagtggc
     4621 ctcgcctatt cccggtattt taagcaggct gccttttttc tgcttaaaaa cggcttcagc
     4681 acttccgcag tatgcaatga gttttttggc gctaatactt ccaacttttg gtattagttc
     4741 gatgccgatt ttatatacca gttcgggatc ggcgctcatt tacctttcac atagctgtta
     4801 agcgcagcaa ccagctgctc aagttgtttt ccggtaagcg aggaaattga aatagccgga
     4861 tacctagaaa ctttatttcc gttccggaca aaaatgctaa gttcgcgacg aaggaacttt
     4921 ttctcgatgg tgaagcggta aaactcgctc ttgtcaacct cgatagaaat tttgggtgct
     4981 ttaaatgggc gaagcagaaa atacctgatg atgatttttt tcccctcatc gttgtacgaa
     5041 aagtagctgg ctcgcctcag gtagaaatag cccgagaaaa ggatgtagag tagcagaatg
     5101 atggcaattg cccaatcccg gtctatccct gttgtcctta gtaaaaaatc gctaaggccg
     5161 gtggctatca atagaacaat taaaataatg gctatgatgg ccattatgag tttggccgaa
     5221 tggataccag ttacggtgtc gtcgttatca aatttcatcc gttagcctta tttttcaaat
     5281 tttatccgaa aaattaatat acatacggaa ctcaaccaag ttgttggcta tgtaaaaata
     5341 gaataatcat ccaacatgcc caacatttat atcattattt aataatgcca acgcctcggt
     5401 ttgtgagatt ttagttttta tggattcttt caggtttctc cttccaccaa tttttccctt
     5461 cgctttacct ctttccttaa aaaatgacgc tcttccctca ggtggctggc tgagtaatgc
     5521 tgaattatgc tgctaacgat tcccttctct actatatttt ttggcccata gggaattgtt
     5581 ttaacaacat tgtagcctga aagggcaagg ttaaagctta aatgatcggc taggccaagg
     5641 tgatgggcaa aaaatacttg agggtcgcct ggactaaccc cgaggttttc cattaaagat
     5701 agaatgtaga gaatggtgtc atggttatgg gtaccaaata taaatgaagc agaggcaagg
     5761 ttgttgagta gaaatttgat tgcctcgttt aagctatggt tgatgtcatt ttgagtattt
     5821 tgaaacaata tcttctcaga ttcgcccacc tcccggtgtg ttagtgtgcc tctaagaagt
     5881 tttgccccaa gtatgtaacc attcttggac gcaatccgaa gctgttcaac tagcctttca
     5941 aacgtctcca ccatgtcaaa cctaaacgtt tgatacacgg ttgccctgtc tccgttatgg
     6001 agcatcatca tttcgctggc aatactatca atagcgggtt gataatcaac atgttcggcg
     6061 tcaataataa tggattttcc ggaactgaaa gcttcgttgc aaagtagcct taccctattg
     6121 gcgaattctg tgtaaatacc tttgtctgca tggtttagca tggcgttttg agataccagc
     6181 cgtagtatcc tgggttcaac taaagtagaa agtttaacca gtgtaaaggc gtggttagga
     6241 aaatccctgg caattcttat agcccttagg tactcatcaa aaaccaactg cttatcattg
     6301 cattcaacaa ggggcgctgg tgaagagtaa actccgtagc cccatagttc gtctccaaca
     6361 ctttcgcagc tttccagcgt taagcctccg gaaaaaaagt cgaatattct atttttgagc
     6421 aggaagttta cgggaggaat tgatgcccac gaattggcct tgccagaacg attcattcca
     6481 cccaattttg atagcagggt gaataacctg catgctcttt tcaactcttt gtttgacaag
     6541 tactggaaag cattaccggt gttatcaagc gctatcatct gactgcaata tttaactcat
     6601 aaagttaaaa caattgtggg aatgtgtgaa aaacagaaaa aacacctata agaaaaagtg
     6661 ttgaaaatgg tttgccgctc cttggcagaa aagaaa
//
LOCUS       DAOOQX010000018         6662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_14067_length_6662_cov_3.636598, whole genome shotgun sequence.
ACCESSION   DAOOQX010000018 DAOOQX010000000
VERSION     DAOOQX010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14067_length_6662_cov_3.636598"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PLX53_00660"
     CDS             <1..475
                     /locus_tag="PLX53_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HOY71620.1"
                     /translation="IGIGGDDRALKAIKGFGLHIFLVPLTTIVGTLTGVAAVYLLLGQ
                     EPFTDMLAVGAGFGYYSLSSIIISEIRGPELGVVALLANVMREIITLLMAPIFARYFG
                     KLAPISSGGATSMDTTLPIITASSGKEYAIVSLFHGIVLTILVPFLVTFILSLQS"
     gene            complement(724..1971)
                     /locus_tag="PLX53_00665"
     CDS             complement(724..1971)
                     /locus_tag="PLX53_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY71621.1"
                     /translation="MKYAALINETFRISIRSITSTKLRSALTILIIALGIMSLVGIIT
                     AIKVIENSISTGFASMGANSFTIQSRGMHVNVNGNQYRTKNYSFISLQQAREFKDKFD
                     FPANVSVAVYATGASTIKYLSNKTNPNISVRGVDENFMMVSGLDVTQGRNYSVTEVND
                     GRNVAIIGNEVARKLFPKNEDPIDKFISVGSGKYRVIGVLKSKGAGFGGGPDRTVFLP
                     YTNVAAYFSRPNMNYSITVQPHDPKLMDAAVGEAEGIFRLVRRLSATDESDFNIEKSD
                     TLAKLLIDNLKFVSIAATLIGIITLVGAAVGLMNIMLVAVAERTREIGTRKAIGAKSP
                     TIKQQFLFEAILICEIGGLLGVFLGILVGNLVSVLAKTSFVVPWGWIFGGLAISFLVG
                     LASGYIPAVRASRLDPIEALRYE"
     gene            2290..3336
                     /locus_tag="PLX53_00670"
     CDS             2290..3336
                     /locus_tag="PLX53_00670"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HOY71622.1"
                     /translation="MKIEERLNAFARLGDKFTKTLSCNEESELGAAIEKAHEQNPWFT
                     PNNVRFALATIAESWLKPDVLNSWIARYPEACFNPQHPVNVGVIMAGNIPLVGFHDFL
                     CVLATGNRITCKLSARDGNLMRSVANTLIQLEPRFSNLIAITDGTLRDFDAVIATGSN
                     NSARYFDYYFGKYPSIIRGHRNGIAVLTGKETQSNLTKLGEDIFTYFGLGCRSVSKLL
                     VPSGYSFTPLLNSFCQWKDLINHHRYANNYEYQRAIFTINQTPHFDTGFLLVTPSEQL
                     DSPVGVLYYQEYNKLDDALKYIEQHDKVLQCVVGLPEIYPKAIPFGSAQNPDIAEYAD
                     EIDTIVFLSQLQAL"
     gene            3394..3912
                     /locus_tag="PLX53_00675"
     CDS             3394..3912
                     /locus_tag="PLX53_00675"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71623.1"
                     /translation="MIYKFRMISSEDRAFLRDFEVDANQTFLTFHEAIQGNLNFDPSQ
                     LVSFFLADEHWNKGMELTLIDMQNEGDMAAIPMDSATVGELIKNRKERLLYVFDIYTD
                     RSLFIELLDIYEPTPGVTYPICTAAVGDPPLQFAGSDIENFSPELADDAGFEDFSDEF
                     DSDEAGSDSIDD"
     gene            3990..4919
                     /gene="miaA"
                     /locus_tag="PLX53_00680"
     CDS             3990..4919
                     /gene="miaA"
                     /locus_tag="PLX53_00680"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOY71624.1"
                     /translation="MSYNGNFLVILLGPTGVGKTDLSISIAKHFEAPIISADSRQFYQ
                     EMRIGTAAPTPSQLAGANHFFIGNKSITERYSCGMFEVDVLNLLETVFTSSRIALLVG
                     GSGLYIDAVVNGIDDFPTPDPELRQALLSQLKNEGVASLRAQLKILDPEYYGKVDLRN
                     SQRVLKALEVCLQTGLTYTSFLTHRAKPRFFNSIFVGLNRPREELYRLINQRVESMMA
                     EGLLDEAKKLYPFREFNALNTVGYRELFSYLDGQTSLEEAIELIKRNTRRYAKRQLSW
                     WSRNKNITWFHPSQLTEITEFIEKTIASYSAKN"
     gene            4926..5642
                     /locus_tag="PLX53_00685"
     CDS             4926..5642
                     /locus_tag="PLX53_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOY71625.1"
                     /translation="MISNEKPNSEIFIELFTEIEQRLKTICNDEFTSNFSELLAKARG
                     KSGVVNRYASDLKEFAQLRNAIVHTRRQNFIIAEPHDDVVSEVIHIHKLLSSPPKVSS
                     VMRHNPFTVFPETPISVVLNTFAEKGFMRCPVVEQGRIVCLITAKTISRWITSIKQDR
                     IEVNNTPVRKLVPFADKSDYMVIAENDDIATLLDLFNESIRKGSYLQAALVTQSGEPQ
                     SKLVGIVTPSDLPLIMEKLK"
     gene            5796..>6662
                     /locus_tag="PLX53_00690"
     CDS             5796..>6662
                     /locus_tag="PLX53_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HOY71626.1"
                     /translation="MSDESVEKIKGLPKNAYTELKDGEEYIPIMPPHKTVPEVNLWSV
                     TWGLVMAIIFSAAAAYLGLKIGQVFEAAIPIAIIAVGLSTMGKRKGALGENVIIQSIG
                     ASSGVIVAGAIFTLPALYILNLEAQFYQIFLSSLFGGILGILFLIPFRKYFVSDMHGK
                     LPFPEATATTEVLVSGEKGGKQARLLVVSGLIGGIYDFIIATFGWWSEVITSRVIPWG
                     EALADKSKLVFKLNVGAAVTGIGYIIGLKYTAIIAAGSFVGWFVIIPIINYFAPGLTA
                     AVGSNITAVVGSM"
BASE COUNT         1852 a         1619 c         1481 g         1710 t
ORIGIN      
        1 aataggaata ggcggcgacg acagggcgct caaagccatc aagggattcg gcctgcatat
       61 ttttttggtt ccgctcacca ctattgtggg aacgctaacg ggggtagccg ctgtttacct
      121 tctgctggga caggaaccat ttaccgacat gcttgccgtt ggtgccggat tcggctacta
      181 tagcctttcg agcatcatca tcagcgagat aaggggtccc gagctgggag tagtagccct
      241 gctggccaac gtgatgcgcg aaatcataac gctgctcatg gcgcccatat ttgcccgata
      301 ctttggcaag ctagccccca tatcatcagg cggggcaacc agcatggata ccacccttcc
      361 aatcattacg gcctcatcgg gcaaggaata cgccatcgta tccctgttcc acggaattgt
      421 tctcaccatt ctggtcccct ttctggtaac gtttattctt tcgctgcaaa gctgaaattg
      481 acaccaaccc tacaaataag gaagtatatt ttggcttttg ttgtttcctg gaaataccta
      541 gctattaagg gaacaaatca aaaacagcaa tggaattgag gctaaatgtt tacggattac
      601 agagattagg gagatgcacg taggttctgt cagtacaaat ttccctaata ccctccgttt
      661 ttactgtggt taaattcccc tctaccataa cataaaggga aataagcaaa aagctagatg
      721 cccctactcg taacggagcg cttcgatggg atcaaggcgc gaagccctaa ccgcaggaat
      781 gtaacccgac gctaaaccaa ccaaaaagga aatggccagc cctccaaaaa tccatcccca
      841 gggaacaaca aaggaggttt tggcaagtac cgacaccagg tttcctacca ggatgcccag
      901 gaaaacacca agcaacccac caatttcgca tatcagaata gcctcaaaaa ggaactgctg
      961 cttgatggtg ggtgattttg cccctattgc cttgcgcgta ccaatctccc tggttctctc
     1021 ggcaaccgcc acaagcataa tgttcatcaa accaacggct gctccaacca gggttattat
     1081 cccaataagg gttgctgcaa tggaaacaaa cttgaggtta tctatcagca acttggccag
     1141 cgtgtcgctt ttttcaatgt tgaaatcgga ctcatcggtg gcggaaagcc tgcgaaccag
     1201 cctaaaaata ccttcggcct cgccaacagc tgcatccatc agcttagggt cgtggggctg
     1261 aacagttatg ctgtagttca tgttgggacg tgaaaaatac gccgcaacat tggtgtaggg
     1321 caggaaaact gttctatcgg gaccaccgcc aaaaccagcc cctttcgatt tcagaacccc
     1381 tatcacccgg tactttccgc tgcccacgct aatgaacttg tcaattggat cttcattttt
     1441 gggaaaaagc ttacgggcta cctcgttacc aattatggca acatttcttc catcgtttac
     1501 ctcggtaacc gaatagttcc gaccctgcgt aacatccagg cccgacacca tcataaagtt
     1561 ttcgtcaaca cccctgacgc ttatgttggg gttggttttg ttggataagt acttgattgt
     1621 gctagcccct gttgcgtaaa cagctacgct gacattggct ggaaaatcaa atttatcctt
     1681 gaactccctc gcctgttgaa ggctgataaa ggagtagttc ttggttcgat actggttacc
     1741 gtttacgttt acatgcatcc ccctgctctg aatggtaaac gagtttgccc ccatgctggc
     1801 aaatccagtg cttatgctgt tctcaatcac cttaatggca gtaattattc caaccagcga
     1861 catgataccc aacgcaatta tcagtatggt aagcgcagac cggagctttg tagaagtaat
     1921 agaccggatt gaaattcgaa atgtttcgtt gattagggcg gcgtacttca caacacttaa
     1981 attaggttaa acaaatagtc agcacctcat tttctcatga aaacataaaa caacatcgtc
     2041 ttaatgcgaa gctataattt gatgggtgtc ccacatcaaa tccttttctt ctaaagcatt
     2101 gctgtttttc atatgctgcc tgttccgttg aaaaactttc aaagttatta atttatacca
     2161 attttcgtcc gaaaagttaa ggtcaataca aaacccttgc agcaggatga agcctaccct
     2221 gcacagctca aacattgcgg tccgagccaa aataatacta gctttgcaac ataaagacgc
     2281 agtgttaaga tgaaaataga ggaaagacta aacgcgtttg ctcgtttggg tgataaattt
     2341 accaaaacgc tatcgtgcaa tgaagagtcg gaactgggtg ctgccattga aaaagcgcac
     2401 gagcaaaatc catggtttac ccccaataac gttcgatttg ctttagctac tattgccgaa
     2461 agctggctta aacccgatgt ccttaacagc tggatagcgc gctaccctga agcctgcttc
     2521 aacccgcagc atccagttaa cgttggtgta atcatggccg ggaatatacc tcttgttggg
     2581 tttcatgatt ttctttgcgt gctggcaacc ggtaaccgga ttacctgcaa gttgtccgca
     2641 agggatggca acctgatgag gtctgtggca aataccctga tccagcttga gccgcgcttt
     2701 tcaaacctta tagccatcac cgacggcaca ctaagggatt ttgatgcagt aattgctacg
     2761 ggtagcaaca actccgcccg atattttgat tactactttg ggaagtaccc atctattatc
     2821 aggggccacc gcaacggaat agccgtgcta acaggaaagg aaacccaaag caatttaaca
     2881 aagctaggag aggacatttt cacctacttt ggcctgggct gccgaagcgt ttccaagcta
     2941 ttggttccat cggggtatag ctttactccc ctgctcaaca gcttttgcca gtggaaagac
     3001 ctaatcaacc accaccgcta tgccaataac tacgagtacc agcgtgcaat cttcaccata
     3061 aaccaaacgc cccattttga cactgggttt ctgctggtaa ccccttcaga gcaactcgac
     3121 tctccggtgg gggtacttta ctaccaggag tataataaac ttgatgatgc tctgaagtac
     3181 attgaacagc acgataaagt attgcaatgc gttgtaggat tacccgagat ttacccaaaa
     3241 gccattccct tcggatcggc tcaaaaccca gatattgcgg aatatgcaga cgagatcgac
     3301 acaattgtat ttctttcaca actgcaggct ctttaacttt gaatatttgc atttattcac
     3361 taaaactgct tattttgtag caaagtataa agtatgatat acaaattcag aatgatatcg
     3421 tcggaggaca gggcattcct tcgggatttt gaggttgatg ccaatcaaac cttcctaacc
     3481 tttcatgagg caattcaggg aaacttgaat tttgacccat cgcagctggt atcattcttt
     3541 cttgccgatg aacactggaa taaaggcatg gaacttaccc taatagacat gcagaatgag
     3601 ggtgatatgg ctgctattcc catggattct gcaaccgttg gcgagctgat aaagaatcgc
     3661 aaggaacgcc tgctttacgt tttcgacata tacaccgatc gtagcctatt tatcgagctg
     3721 ctcgacattt acgagccaac tcccggagtt acctacccta tctgcactgc agcagtgggc
     3781 gatcctccac ttcaatttgc aggtagcgac atcgaaaatt tcagccccga gttggccgat
     3841 gatgctggat tcgaagattt ttccgatgaa ttcgattccg acgaggctgg tagcgactcc
     3901 attgacgatt aacccgcata aactcggtat aaaagcttca taaagcatag ctgaaacgag
     3961 ggcggtttta gccgcttcca tgaaaaataa tgagctataa cggcaatttt ttggtaatcc
     4021 tgcttggccc cacaggtgtt gggaaaacag acctgagcat ttccattgcc aagcactttg
     4081 aagcgcccat tatatcagct gattcacgtc agttttacca ggaaatgagg attggaactg
     4141 ctgcacccac ccccagccag ctggccggag ctaaccactt ctttattggc aacaagtcta
     4201 ttaccgagcg gtatagctgt ggaatgttcg aagttgatgt gctcaaccta cttgaaaccg
     4261 tttttacatc aagcagaatt gccctactgg taggaggttc ggggctttac atcgatgctg
     4321 tagttaacgg aattgacgat tttcccactc ctgaccccga gctacggcag gccttactca
     4381 gccagctaaa aaatgaaggg gtagccagcc tgcgagcgca gctaaaaatt ttggatccgg
     4441 agtactatgg aaaggttgac ctgaggaatt cccagcgcgt gctaaaagcc ttggaggttt
     4501 gcctgcaaac tggtctcacc tacacctcat tccttaccca tcgggcaaag cccagattct
     4561 tcaattcaat ttttgtgggg cttaaccgtc cacgggagga actctaccgg ctaataaacc
     4621 aaagggttga atccatgatg gctgaggggt tgttggacga agcaaaaaaa ctttaccctt
     4681 ttagagaatt caatgccctt aacaccgttg gctaccgcga gctattcagc tacctcgacg
     4741 ggcaaacaag cctcgaggag gctattgaac ttatcaaacg aaatacccga cgctacgcca
     4801 agcgtcagct aagctggtgg agccgaaata aaaatattac ctggttccat cccagccagc
     4861 tgactgaaat tacggaattc atcgaaaaaa ccattgccag ctattcagca aaaaattaaa
     4921 aaaatatgat ctcgaacgaa aaaccaaact ccgaaatttt tatcgagctg ttcaccgaga
     4981 ttgagcaaag gctcaaaacg atttgcaacg acgagttcac cagcaacttc tcggagctgt
     5041 tagcgaaagc acgtggcaag agtggtgtgg taaaccgtta tgcatctgat ttaaaagagt
     5101 ttgcccagct acgcaatgcc attgttcaca cccgccgcca aaacttcata atagccgaac
     5161 cccacgatga tgtggtgagc gaggttatcc atatccataa gctgctaagt agccccccaa
     5221 aagtatcgtc ggttatgagg cataatccat ttaccgtttt ccctgaaaca ccaattagcg
     5281 ttgttctaaa cacctttgct gaaaaaggat tcatgcgctg ccctgtagtg gagcagggta
     5341 gaattgtttg cctgatcacc gctaaaacca tttcgcgttg gatcacctcc attaagcagg
     5401 ataggattga agtgaataac accccggtta gaaagctcgt cccgtttgcc gacaaatcag
     5461 actatatggt tatagctgaa aatgacgaca ttgctactct tctcgacctc ttcaacgaat
     5521 ccatcaggaa gggaagctac cttcaggcag cgctggtaac ccaaagcggc gaacctcaaa
     5581 gtaagctcgt tgggattgtt acgcctagcg acctaccact tatcatggaa aaattaaagt
     5641 agaaccgggc agtagagttt cgagcgcttt gatgaggagt ttcctcagaa tcacgctcgt
     5701 aataagaagt tgaatcactg aaaagattta ctttttttct tctatttttg ccaactagct
     5761 aatcatccta cctaaaatct taaaaataaa aaaacatgag cgacgaatcg gttgaaaaaa
     5821 ttaaagggct ccctaagaat gcctacaccg agctaaagga tggcgaggag tacattccaa
     5881 taatgccgcc ccataaaact gttcccgagg ttaacctctg gagcgtaaca tggggactgg
     5941 taatggccat aatattctcg gcagcagcgg cctaccttgg actaaaaatc gggcaggttt
     6001 ttgaagcggc cattcccatt gccataattg ccgtaggcct gtcaaccatg ggaaaacgaa
     6061 agggtgctct tggtgaaaac gtcatcatac aatccattgg agccagctcg ggggtaattg
     6121 ttgcaggtgc catattcacc cttccggcgc tttacatcct aaacctcgag gcgcaatttt
     6181 accagatttt cctatcgtcg cttttcggag gaattcttgg catccttttc ctgataccgt
     6241 ttcgaaagta ctttgtatcc gacatgcacg gaaaacttcc gtttccggag gcaacagcca
     6301 ctaccgaggt gctggtatcg ggcgaaaagg gaggcaagca ggcgcgcttg cttgtggtta
     6361 gcggtctaat aggtggtata tacgatttta taattgccac attcggttgg tggagcgagg
     6421 tgattacctc cagggtaatc ccatggggcg aagccttggc cgacaagtca aaactcgtat
     6481 ttaagttgaa tgtaggtgct gccgttacgg ggattggata catcatcgga ctgaagtaca
     6541 ctgctatcat tgcagcaggc tcgttcgttg gttggtttgt tattatcccc atcatcaact
     6601 attttgcccc tgggttaacc gcagcagtag gaagcaacat aaccgcagtg gtgggaagca
     6661 tg
//
LOCUS       DAOOQX010000019         6615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_14179_length_6615_cov_3.270732, whole genome shotgun sequence.
ACCESSION   DAOOQX010000019 DAOOQX010000000
VERSION     DAOOQX010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14179_length_6615_cov_3.270732"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PLX53_00695"
     CDS             <1..589
                     /locus_tag="PLX53_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71627.1"
                     /translation="QADVEPYKVKVSYPKDSVWKVVAANDLFAYRELKNPSRIIVKSF
                     DRKHYEINRELKDPFSPTVNLVVDDSLMGVIDTLNAVAPHARYHSVFYGMGMGYHGTP
                     TFEGPLLRPLVENFLAKDGAKWMRTGIACVVGKDGFRNIFSVSELFNRVDQVEPILAI
                     PENPKKSGYYRLYHPSAFYADFSVRNLSEIYLFRE"
     gene            complement(656..1585)
                     /gene="miaA"
                     /locus_tag="PLX53_00700"
     CDS             complement(656..1585)
                     /gene="miaA"
                     /locus_tag="PLX53_00700"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOY71628.1"
                     /translation="MDNCPYDIIAIVGPTASGKTLLAANLASRVNGEIISADSRQVYR
                     GMNLGTGKDYEDYVVNGKTIPYHLVDLVDAGYHYNVYEYQSDFLKVFRSLKARKVLPI
                     LCGGSGLYIEAVLRGYRLLPVPQNATLRGELEAKSNDELAKILASYKTLHNTTDLDTR
                     KRTIRAIEIEEHYLNASTEIEPFPQLKSAIFGVRYNRATEMERIHQRLLQRLGNGMVD
                     EVRGLMESGLSFDDMAYYGLEYRYIAEHIMGKLTYDEMVSRLNIAIRQFAKRQMTWFR
                     GMERRGLSITWINGELPLEDKLNKIMEAIQNGF"
     gene            1705..1938
                     /locus_tag="PLX53_00705"
     CDS             1705..1938
                     /locus_tag="PLX53_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71629.1"
                     /translation="MAALRRNNRQSRHEELNRLIWMGIVIAVTFIFVIAVATDMFRII
                     PLKRTNIVMLVLALLIVAVYVYSWIKFYRKRKQ"
     gene            1951..2400
                     /gene="smpB"
                     /locus_tag="PLX53_00710"
     CDS             1951..2400
                     /gene="smpB"
                     /locus_tag="PLX53_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOY71630.1"
                     /translation="MSNEIVVKNKKATFQFEIVEKFTAGIVLKGTEIKSIRMGRASFV
                     DSYCFFIGDELWVKGLHIAEYAWGTYNNHDPKQDRKLLLTRKELTKLKRRSQEKGLTI
                     IALKLFINERGLAKVDIALARGKQLHDKRDDLREKDVRRELDRARKR"
     gene            2538..3137
                     /locus_tag="PLX53_00715"
     CDS             2538..3137
                     /locus_tag="PLX53_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOY71631.1"
                     /translation="MGKVKLTVLGISYSQTQSGAYALVLSEEDGNRRIPIIIGGYEAQ
                     AIAIQLEGLTPPRPLTHDLFMNFAKAYHIEILDVNIYKLEEGVFFSKLHCTNGDKEIF
                     VDSRTSDAVALALRFDCPIYTTEEIIEKAGLTLEIENIDEDAAKEDEGEKGNAPVDSF
                     MEQLAKLSVSELKTMLDEAVRNEDYEKASKIRDELKNRS"
     gene            complement(3220..4461)
                     /locus_tag="PLX53_00720"
     CDS             complement(3220..4461)
                     /locus_tag="PLX53_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HOY71632.1"
                     /translation="MKLKLRLTLMSFLQYAIWGSWLISLGAYLGGGLHFSGFQIGSFF
                     ATMGIASLFMPAIMGIIADRWIPAQKVLGICHILAAAFLIAAAPQTQYGTLYPLVLLS
                     VMFYMPTIALSNSVAYNALSLNGYDTVKAFPPIRVWGTVGFICAMILVDLLGFATTSY
                     QFYLSAGLGLILGIYSFTLPNCEVNKNVEKKSLVDSLGLRAFTLFKERKMAVFFIFAM
                     LLGMSLQITNAYANDYLTNYFGEFPEYQGTFGVKHANILISLSQISETLCILLIPFFL
                     SRFGIKKVMLISMFAWVLRFGLLGLGNPGSGVWMLILSMIVYGVAFDFFNISGSLFVE
                     NETDAKIRSSAQGVFMIMTNGFGAFIGSYIAGLVVDATDWPDAWFVFSAYSLVVAIVF
                     AFVFKYKHNPKNSSTVSKAAQ"
     gene            5003..6073
                     /gene="lpxK"
                     /locus_tag="PLX53_00725"
     CDS             5003..6073
                     /gene="lpxK"
                     /locus_tag="PLX53_00725"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOY71633.1"
                     /translation="MRQIRLLLLPIAWLYWCGVRIRNILFDAGILKGFSPDIPTICVG
                     NLAVGGTGKTPMTAYLSSLLHGNFNVAVLSRGYKRKTKGFKIVSTESTVHEVGDEPLL
                     LKLRQPSAIVAVDEDRMNGMVELFYAYPELDAIILDDAFQHRWIKPGLNLLLTEYENL
                     YTHDFPLPAGRLRDSLSERKRANLIIVTKCPENLTADRCREISAELKPNPTQIIYFTS
                     MEYGMPQPVFSESAGKLKLTDMQPVFAMTGIANPKPFVDYLQKHTALLAAKAFADHHY
                     YSEDEIEAIFTELARSTSQAVVVTTEKDAVRLLKLKLPEEIKKRIYYIPVSVKFLFNS
                     SEEFNNQILSYVRENKSDSSFH"
     gene            6036..>6615
                     /locus_tag="PLX53_00730"
     CDS             6036..>6615
                     /locus_tag="PLX53_00730"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOY71634.1"
                     /translation="MLEKINQTVAFIKGKVTIEPEVGIILGTGLGGLVKEIEKQQALD
                     YKDIPNFPISTVEGHTGRLIFGELGGKKVVAMQGRFHFYEGYDMKQVTFPVRVMKFLG
                     IQYLFVSNASGGMNPAFEIGDLMIINDHINLMPNPLLGPNISELGPRFPDMHRAYDEK
                     LIALAKKVAASNGPAIRKDATVGPTGETLKTPR"
BASE COUNT         1946 a         1529 c         1487 g         1653 t
ORIGIN      
        1 acaggccgac gttgaaccct acaaggttaa agtcagctac ccgaaggaca gtgtttggaa
       61 ggtagtggca gccaacgact tgtttgctta ccgcgagttg aaaaatcctt caagaataat
      121 tgtgaagtca ttcgacagga agcactatga aatcaacagg gagctgaaag atccatttag
      181 cccaacggtt aacttggtgg ttgacgactc cctgatggga gttatagaca ccctgaacgc
      241 agttgcacct catgcccgct accactcagt gttttacggt atgggcatgg gctaccacgg
      301 tacgccaacc tttgagggac cgctgcttcg acccttggtt gaaaatttcc tcgccaaaga
      361 cggggcaaaa tggatgcgga caggcatagc ctgcgtggtg ggaaaagatg gctttagaaa
      421 catatttagc gtaagcgagc tgttcaaccg ggttgatcag gttgaaccga ttctggccat
      481 acccgaaaat ccgaagaaaa gtggttacta ccgtctttac cacccgtcag ctttttacgc
      541 cgatttctcg gtaagaaacc tgtcggagat ttacctgttc cgggagtgag agggttgctg
      601 tgttgcccct cctgtttgga atgccgtttt atcgggaaaa gcatcattca aacagctaga
      661 aaccattttg aatggcctcc attatcttgt tcagcttatc ttccaaaggg agttcgccat
      721 taatccaggt aatgcttagc ccacggcgtt ccatgccacg aaaccaggtc atttgccgct
      781 tggcaaattg cctgatagcg atgttcaagc ggctaaccat ttcgtcgtag gttaacttgc
      841 ccatgatgtg ctcggcgatg tagcggtact ccaagccgta gtaagccata tcgtcaaaac
      901 ttagccccga ctccatcaac cccctcacct catctaccat accgttcccg agccgttgta
      961 gcagccgctg atggattcgc tccatttcgg tagcccggtt ataccttacg ccgaatatgg
     1021 cacttttaag ctgagggaat ggttcaattt cggtagaagc gttcaggtag tgctcctcaa
     1081 tttcgatagc cctgatagtt cgcttacggg tgtcgagatc ggtggtgttg tgaagagttt
     1141 tgtaggatgc aagaatttta gccagctcgt cgttcgactt agcctcaagc tccccacgga
     1201 gggttgcgtt ctggggaact ggcaaaagcc ggtaacccct taacacagcc tctatgtata
     1261 gcccgcttcc tccacaaagt ataggcaaaa ccttgcgcgc tttcaggcta cggaatacct
     1321 tcagaaaatc ggactggtac tcataaacat tgtagtgata gccggcatca accaagtcaa
     1381 ccagatggta gggtatcgtt ttgccattta ccacgtagtc ctcatagtct ttaccggttc
     1441 caaggttcat gccgcggtaa acctgtcggg agtcggcgct aataatttct ccatttaccc
     1501 gcgatgccag attagctgca agcagggttt taccactagc agttgggcct acaatcgcaa
     1561 taatatcgta cgggcaattg tccatttatc ttttgggtga taaagcgtaa atttagctat
     1621 ttttgcgccc tacaaacatt ctaacagcca agtcaccatc ctatcccaat ttataatttt
     1681 atccaccttt aaataaccag caccatggca gcgctacgaa ggaataaccg gcaaagcagg
     1741 catgaagagc taaacaggct aatctggatg ggaattgtaa ttgccgttac attcatattt
     1801 gttattgctg ttgcaacaga catgttcagg ataattccgc ttaaacgaac caacattgta
     1861 atgctagtgt tggcattgct aattgtggcg gtttatgttt acagctggat aaagttttac
     1921 agaaaaagga agcagtagcc acatactccc atgagtaacg aaattgttgt taaaaacaag
     1981 aaggcaacct ttcagttcga gattgtcgaa aagttcaccg ccggaattgt gcttaaagga
     2041 acggaaatta aatccatccg aatgggcagg gccagctttg tggattcgta ctgctttttc
     2101 attggtgatg aactatgggt taaggggctt catatagcgg agtatgcctg gggtacctac
     2161 aacaaccacg accccaaaca ggatagaaag ctgctgttaa ccagaaagga gctcactaag
     2221 ctgaaacggc ggtcacagga gaaaggactc accattatag ccctgaaact ttttatcaac
     2281 gaacggggtt tggccaaggt tgatattgct ttggcacggg gaaagcagct gcacgacaag
     2341 cgggacgacc tgagggaaaa ggacgttcgt cgtgagctcg atcgtgcccg taaaaggtag
     2401 ccatcatctt ttttcttacc ctgtttcttc taaaggattt tcattctcaa gctaattacc
     2461 agcgcataaa attcaaattt tgaaaaaatt ccattaggtt tgtaaagtgc aataaaaaag
     2521 gataaccagc aaagcaaatg ggtaaggtaa agttaaccgt tttaggtatt tcatacagtc
     2581 aaactcagtc tggtgcctac gccttagtct tgagcgaaga agacgggaac aggagaattc
     2641 cgataataat tggtggctac gaggcgcaag ccattgcaat tcaactcgag gggttaaccc
     2701 caccacgccc gttgacccac gacttattca tgaattttgc taaagcttac catatagaaa
     2761 ttctggatgt caacatctac aagctggagg agggtgtgtt tttttcaaag ctacactgca
     2821 ccaatggcga taaggagata tttgttgatt ccagaacctc cgatgctgta gcgttggcac
     2881 taaggtttga ttgccccatc tacaccaccg aggaaataat tgaaaaagcc gggcttaccc
     2941 tcgaaattga gaatattgac gaggatgccg ctaaggagga tgagggagaa aagggaaatg
     3001 cccccgttga cagcttcatg gagcaactcg ccaagctatc tgtttcggaa ctaaagacta
     3061 tgctcgacga agccgttcgg aacgaggact acgaaaaggc ttcaaaaatt cgcgacgagc
     3121 taaagaaccg ctcctgaaaa aaataaagtc aaacattacc tatgggcatc aagcagaagc
     3181 cccatcctgg caggatgggg cttctggcaa aatacttctc tattgtgcag ccttacttac
     3241 ggtggacgaa ttctttgggt tgtgcttgta cttaaaaaca aacgcaaaca caattgccac
     3301 taccaacgag taggccgaaa atacaaacca ggcatcaggc cagtctgttg catcaacaac
     3361 cagccctgca atataggagc ctataaatgc tccaaaacca ttggtcataa tcataaaaac
     3421 cccctgcgcg ctggaacgga ttttcgcatc ggtctcattt tccacaaaaa gtgaccccga
     3481 aatgttgaaa aagtcgaatg caacgccata cacaatcatg gaaagaatca gcatccaaac
     3541 tcctgaacct gggttcccca accccagcag accaaaacga agtacccatg caaacattga
     3601 tatcagcata acctttttaa taccaaaccg gctaaggaaa aacggtatca gcagaatgca
     3661 aagggtttct gaaatctgcg atagcgagat gagtatgttg gcatgtttaa caccaaatgt
     3721 tccttggtac tctggaaatt caccaaagta gttggtgagg tagtcgttgg cgtaggcgtt
     3781 ggttatctgc aacgacatgc ctagcagcat ggcaaaaata aagaatacgg ccatcttgcg
     3841 ctccttaaac aaggtaaagg cacgcagccc taaagagtca accagcgact ttttctctac
     3901 atttttattc acctcacagt tggggagcgt aaatgagtaa attcctaaaa ttaaccccag
     3961 ccccgccgaa aggtaaaact ggtaggaggt tgtggcaaaa cccagcaggt caaccaaaat
     4021 catagcgcaa ataaaaccca ccgttcccca tacccgaatg ggaggaaatg cctttaccgt
     4081 atcgtaccca ttgagcgata aggcattata ggcaaccgag tttgataggg ctatagtagg
     4141 catgtagaac ataacgctta gcagtaccag tggatacaag gtgccgtact gcgtttgggg
     4201 agcagcagca atcagaaagg cggctgcaag aatatggcaa atgcccagca ccttttgggc
     4261 aggaatccac ctatcggcaa tgatgcccat tattgcaggc atgaaaagcg aagcaatacc
     4321 catcgttgca aaaaaactcc caatctggaa acccgaaaaa tgtaagcctc ctcccaaata
     4381 tgcaccgagg gaaattaacc acgaacccca aatggcatac tgcagaaagc tcatcaacgt
     4441 taaacgcaac tttaacttca taacttttta tatttagcgt tatcgatttt tttaaagtcc
     4501 taaaaatagc attgttttat ttataaggtt ttcgataagg gtagaaatgt tttctgttaa
     4561 aagggcatat cacagcataa actaaacata tccaataaat attttatgaa atttaccgca
     4621 tttttccacc cttttgcata acaatgatgg ccatatttaa ttttaaccca caagcacatg
     4681 ctaaagcacc actagctcaa gcccaatttc taaaaatatc gtcggaatga atacggcaac
     4741 acctcagcag atactcacca aagcaaacag gctaaagctt aaatacccgg tttgcttttc
     4801 cattgctacc atgtaggctg gtagctgcag ccaatccgca ttgaacctgc aatatgtttt
     4861 ttttcttcta catcctgaat gtggaagatt ttccactaaa tttcgggcaa agcgctgtag
     4921 tgttgtcaat aaaatataat tttatcccaa atttattgct ggctttgatt gctagcctac
     4981 aataagaaag ttcaacatta acatgcggca aatcaggcta cttttacttc ccattgcatg
     5041 gctttactgg tgcggagtaa gaattcgcaa catcctgttc gatgcaggta ttctcaaggg
     5101 tttcagtccc gacattccaa ccatttgcgt gggtaacctg gctgtagggg gaaccggcaa
     5161 aacacccatg acggcctacc tttcatccct actgcatgga aattttaatg ttgcagtgct
     5221 aagccggggg tataaaagaa agaccaaggg tttcaaaata gtaagtaccg aatcaaccgt
     5281 tcacgaagta ggcgacgagc ctcttttgct aaaactccgc caaccgtcgg ctatcgtagc
     5341 cgttgacgag gaccgtatga atggcatggt ggagttgttt tacgcctatc ccgagctgga
     5401 tgcaattatc cttgacgatg cctttcagca ccgttggata aaaccaggat taaacctgct
     5461 tttgaccgaa tatgagaacc tttacaccca cgattttccc cttccggcag gcaggctacg
     5521 cgacagccta tcggagcgaa aacgtgcaaa tctcattatt gttaccaaat gccccgaaaa
     5581 cctcaccgcc gataggtgca gggagatttc cgcagaactt aagccaaacc caactcaaat
     5641 catttacttc accagcatgg agtacggtat gccccaacca gtatttagcg aaagcgcagg
     5701 gaaacttaag ctaaccgaca tgcaaccggt ttttgccatg acgggaattg ccaaccccaa
     5761 acctttcgtt gattacctgc aaaagcatac agcattgctt gccgccaaag catttgccga
     5821 tcaccattac tattccgaag atgagatcga ggctattttt acggagcttg ccaggtcaac
     5881 cagccaggct gtagtggtta caactgaaaa agacgcagtt cgcctgctga agttgaagtt
     5941 gccggaggaa attaaaaaac ggatatacta tattccggtg agcgtgaagt ttctattcaa
     6001 cagttcagaa gaatttaata accaaatact tagttatgtt agagaaaata aatcagacag
     6061 tagctttcat taaggggaag gttaccattg aacccgaagt tggaattatt cttggaaccg
     6121 ggcttggcgg cctggtaaag gagatagaaa agcaacaggc attggattac aaggatattc
     6181 caaactttcc gatatcaacg gttgaagggc acaccggacg cctgattttc ggtgagctag
     6241 gtggtaagaa agtggtggcc atgcagggac gcttccactt ctacgaaggt tatgatatga
     6301 agcaggttac cttccctgtg agggtgatga agtttctggg catccagtac ctttttgtat
     6361 cgaatgccag cggtggtatg aatcccgcct ttgaaattgg tgacctgatg atcatcaacg
     6421 accacatcaa cctgatgcct aatccccttc ttgggcctaa tataagtgag ctgggtccaa
     6481 gattcccgga tatgcatagg gcttacgatg agaagctgat tgccctggca aaaaaggttg
     6541 cagcatcaaa cggcccggca ataaggaagg atgctacggt tggacccacc ggcgaaacct
     6601 tgaaaacccc aagga
//
LOCUS       DAOOQX010000020         6539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_14386_length_6539_cov_3.638803, whole genome shotgun sequence.
ACCESSION   DAOOQX010000020 DAOOQX010000000
VERSION     DAOOQX010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14386_length_6539_cov_3.638803"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLX53_00735"
     CDS             complement(<1..586)
                     /locus_tag="PLX53_00735"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOY71635.1"
                     /translation="MELPFAESYKIKMVEPIHRSTRSERERWIKEAKYNMFNLKSEQV
                     FIDLLTDSGTGAMSDRQWSALMMGDESYAGASSYYNLKSAIQNILGFEHFLPTHQGRA
                     AENVLFSVMVKEGDYIPGNSQFDTTKGHIEFRKAEAVDCTIDEAENTEIYHPFKGNVD
                     PQKLDDFIRTHADRVPFVIITITNNSAGGQPVSMQ"
     gene            complement(845..1387)
                     /locus_tag="PLX53_00740"
     CDS             complement(845..1387)
                     /locus_tag="PLX53_00740"
                     /inference="COORDINATES: protein motif:HMM:NF027675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HOY71636.1"
                     /translation="MRKLLRVLHRDFGYFFTGMTLIYCVTGILLNHTRDWNPYYSIDI
                     RKVELNLPLDTSLINKELLTSKLEPIGVQGLRSFYYPKPGTVKLFVSDGTVTSDLGSG
                     SALVERSYRRPVLYQLNMLHKNLPKSWWTWFSDIFAIALMAITISGLFLVKGKNGITH
                     RGVILIIAGLALPLVAIILL"
     gene            complement(1504..1956)
                     /locus_tag="PLX53_00745"
     CDS             complement(1504..1956)
                     /locus_tag="PLX53_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71637.1"
                     /translation="MMKKVFLLAATAASILACSCGTKSSSDNQQAKADSTAASIVEVS
                     IDSLISNPQAFANQTVKFTAKVDHVCMHSGQKLTLMGSDSGSTFKVMATDAVPQFDQN
                     LNGTTVEVVGVVQGAAPEDVESCSEEEGGQQKAKKLDYAVFCQKVRAL"
     gene            complement(2017..2877)
                     /locus_tag="PLX53_00750"
     CDS             complement(2017..2877)
                     /locus_tag="PLX53_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOY71638.1"
                     /translation="MVKTIRNYLSLVKFSHTVFALPFAVIGFFLAYRQTPQNVDVWLF
                     VLVVLCMVFARNAAMSFNRLVDRRFDAINPRTQNREIPRGIITSRATAVFVTVNVLLF
                     IATTYFINRTVFFLSPIALAIVLLYSYTKRFTMFCHFVLGLGLALAPIGAYLSVTPRF
                     ELLPLLYSVIVLFWVSGFDIMYALQDEDFDKRENLKSLPAALGVKKSLLVANLLHFVA
                     GAVVVLIGILYHSDVWYWVGAALFIALLIYQHLIVKPTDISRVNMAFATTNGLASIIY
                     ATFNVISIFF"
     gene            3232..5475
                     /locus_tag="PLX53_00755"
     CDS             3232..5475
                     /locus_tag="PLX53_00755"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY71639.1"
                     /translation="MSRFGKHTTIVLAFWLFMQFPTLQAQVSPDSSLTSKSEEKVLAN
                     LISEGRDNVVKNPDKALDNGLQALVLAKKLGDTRFEIEALLILAESNFTLGQYENSSR
                     YYNRALNFYDQLGNSDAKYKILIGLGRVYDAEKNPELAISTLERGLASDENINNINLY
                     IDLLELLGELYQKQGNYRKSAEMYSKVLAIVDNPMGRKVLLKEKIKSDAFFKLGQVKK
                     NSGELVESLSNFKQAAEISINNFDSLSYSNALQEIALTYYLLQKPDSANFYFTASYAI
                     SNKISDTVGMIQGLQGIADIYLDSGNPTQALYYYNQQLTVAQKLKNIPAITESLVKIS
                     RCYLSSGDISNASKYLNRALAIARQNNLTQSVADVYQYLAILNEGQGRYRDALEFYKM
                     WVETRDSIYFEETGQKLAKLKILYEISQKEKENELLRQNSEIQNLQLNKTRYQTRILI
                     LIIIIFITLIVLLIILFRSKQKEIAKRLETEQRITDLNRELERRMIQEIKKQEKQQLL
                     LAQKSKLESLGTLAAGIAHEINQPLGGISMGLDNILLKVQDKSYNDEHMKEKIASLFE
                     NVERIEKIIDHIRYFSRAQKPITFTQININDVVNSSLFMVSTQYENHGVKMEVTLDEN
                     IGSITADKYKLEQVILNLLSNAKYAVDEKGKKHANHQYTKLIEIKTWQDNNQVYLSVR
                     DNGMGIPDKIIDKIFDPFFTTKSEERGTGLGLSVSYAFIKDLLGDIKVESIEGEYTRF
                     EISLPKM"
     gene            5482..>6539
                     /locus_tag="PLX53_00760"
     CDS             5482..>6539
                     /locus_tag="PLX53_00760"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOY71640.1"
                     /translation="MKEIRILVLDDEKVFRDEIKEFLENDGFTVLLSDRPSAAFEMLT
                     QQEVDIMILDIKLPEMDGFEVLRRVKEEYPDVEVIMITGHGDMEAVIQAMRLGAVEFF
                     PKPFRLLDMRAAIQRTKRYITISNQLKEINQTYSLISKELIESVGNEILGTSPAISHV
                     VNLMGRVAKTENTSVLITGESGAGKELVARGIHYLSNRKDSYFYAVNCSAIPESLFES
                     EFFGHKKGSYTGANEDKAGWFEVANGGTLFLDEIVEMLPAMQSKLLRVLEERKVRRIG
                     SSVDIPVDVRIIAATNQNVFTQLETGKLRSDLYYRLNTFEIHIPPLRERREDIPVLLD
                     YYVKFFARKLGKRISEIDP"
BASE COUNT         1998 a         1493 c         1429 g         1619 t
ORIGIN      
        1 tttgcattga tactggctgt cctcctgctg agttattggt aatggtgata atgacaaatg
       61 gaaccctgtc ggcgtgggta cggataaagt catcaagttt ctgggggtca acatttcctt
      121 taaaagggtg gtaaatctct gtattctctg cctcatcaat ggtgcagtca accgcctcgg
      181 cttttctaaa ttcaatatgc cccttggtgg tatcaaactg cgagttgcca ggaatgtagt
      241 cgccctcttt caccatcacg gagaaaagga cattctcggc agctctcccc tgatgggttg
      301 gcaaaaagtg ctcaaatccc agaatattct ggatggcgct tttaaggttg tagtatgagg
      361 aagctcccgc atagctctcg tcacccatca tgagcgcact ccactgcctg tcgctcatgg
      421 caccggttcc ggaatcggtg agcaaatcaa taaaaacctg ctcgctcttc aggttaaaca
      481 tgttgtactt ggcctcctta atccaacgct ccctttcgct tcgggtgctc cggtgtatag
      541 gctcaaccat cttaattttg tacgattctg caaatggtag ttccatatcg ataatttttt
      601 agcggaaaaa aattaaaact tagggataac ttaaacagtg ctgcatagcc gggggatggc
      661 tatgcaaatg acggcattag aaaaagttat ccctgttctt gatgttcagg aatacgtact
      721 tggcagaaaa tttaaaatga aggtcgcaat aattcattat gctgaatgcc gatatgatgc
      781 ttacttgtag aagcaaattt agtcaacatt agctaaatat tcctatcaac ccagctaaaa
      841 atgtttagag gagaattatg gccacaaggg gaagtgctaa gccagcaata atgagaataa
      901 caccacgatg ggtaatgcca ttcttccctt ttaccaggaa aagccccgat atagtaattg
      961 ccatcagggc tatggcaaaa atatcgctga accaagtcca ccatgatttg ggtagatttt
     1021 tatgcagcat gttaagctgg tagagaacag gcctgcggta gcttctttcc accagagcgc
     1081 taccgctccc tagatctgaa gtaaccgttc catcggaaac aaatagcttg actgttccgg
     1141 gtttagggta gtagaacgac cttagcccct gtacaccaat aggttccaac ttagaagtca
     1201 gcaactcctt gttaattaat gatgtgtcga gtgggagatt caactccacc ttacgaatat
     1261 caatggagta gtaggggttc cagtcgcggg tatggttgag caaaattccg gttacacagt
     1321 atataagagt cattcccgtg aaaaagtaac caaaatcacg gtgcagcacc ctgagcagct
     1381 tgcgcatgtt catgaacgat taacttatat attttccctg gtataaaaaa accggcaggt
     1441 atcctcctcc accataaggc gtgaaggatt gccaccggag tatagtttaa ttaaggcaga
     1501 atgctagagg gcccttactt tttggcagaa aactgcatag tcgagttttt ttgctttctg
     1561 ctgtccacct tcctcttcgc tgcatgattc cacatcttca ggtgctgcac cctgcaccac
     1621 gccaacaacc tcaacagtgg ttccgttgag attctgatcg aactggggaa cggcatcggt
     1681 tgccattacc ttgaaggtgc tgcccgaatc gctgcccatg agcgtaagct tttggccgct
     1741 atgcatgcaa acatgatcaa ccttggcagt aaactttacg gtttggttgg caaatgcctg
     1801 tgggtttgag atgagagaat caatgctaac ctcaacaatt gacgctgctg ttgaatcggc
     1861 ttttgcctgc tggttgtcag aactgctctt tgttccgcat gagcaggcaa gaatacttgc
     1921 tgctgtagct gcgagtaaaa atactttttt catcatactt ctgtgatttt gagtaaaatt
     1981 tagttgaaaa tgtcctttaa aactagcttg tgcaagttag aaaaaaatac tgataacatt
     2041 aaacgtagca taaataatgc ttgccagacc gtttgtggta gcgaaagcca tgttaaccct
     2101 cgatatatcg gtaggtttaa ctattagatg ctggtatatc agcagggcga taaaaagggc
     2161 tgcacccacc cagtaccata catcggagtg atataggata ccaatgagaa ctacgacagc
     2221 tcctgcaaca aaatgtagaa ggttggccac caaaagagat tttttaacgc ccagtgctgc
     2281 cggtaatgac tttagatttt cccgcttatc aaaatcttca tcctgcaggg catacatgat
     2341 atcaaaaccg gatacccaga atagcacgat aaccgagtaa agtaacggca acagctcgaa
     2401 cctgggggta actgacaagt aggcacctat aggcgccagg gccaatccaa gaccaagcac
     2461 aaaatggcaa aacatggtaa atcgcttggt gtagctgtac agaagcacga ttgccaaagc
     2521 tattggcgaa aggaaaaaca ccgtgcggtt gatgaagtag gtggttgcaa tgaagagcaa
     2581 cacattcacc gtaacaaaca cggctgtagc cctggaggta attatgccgc gaggaatttc
     2641 tctgttttgc gtccttggat taatggcgtc gaaacgacgg tcgactagcc ggttaaagct
     2701 catggcggca ttacgggcaa aaaccataca gagcaccacc agcacaaaca accaaacatc
     2761 aacattctgc ggggtttgcc tataggccaa aaaaaatcca attactgcaa acggcaatgc
     2821 aaatacagtg tggctaaact taaccagcga aagataattc ctaattgttt ttaccataga
     2881 cagaaagaat tgtactcatc agcaccaaat attaaacact ttaaatagta aatatcctac
     2941 aatctactcc tactcatttc ttaaaaaacg tattaacgcc gttgaacaca tgaagcatgc
     3001 tcacataaac aagccgtttc tggttgcctc actttaaacc tactgtgcgt aatgcacata
     3061 actcaacagg atgaataaaa aaacagtaaa acttcaccaa cagtggaaga tcaaaaacat
     3121 tagcaataga ccgatagtac tccgatgaat tcgtccctca ggcaaaaaat gcgatccaaa
     3181 agtagccatt tttttaaatt tgggtatctt tagtgctgca ataaccaccc tatgagccgg
     3241 ttcgggaagc atacgacgat tgttttggcc ttctggcttt ttatgcaatt ccccactctt
     3301 caagcccagg tcagcccgga ttcgagcctt acctccaaat cggaggaaaa agttctggcc
     3361 aacctgatat ccgagggaag ggacaatgtt gtcaaaaatc cagataaggc attggacaat
     3421 gggctacagg ctctagtgct ggcaaaaaag ctgggcgata cccgatttga aatcgaagct
     3481 ctcctgattt tagccgagtc gaactttacc cttggacagt atgaaaattc atcacgctac
     3541 tataataggg ctctcaactt ttatgaccaa cttggcaact ccgatgcaaa gtacaagata
     3601 ctcattgggc ttgggagggt gtatgatgct gagaaaaatc ccgagctggc catttccacc
     3661 cttgaaaggg ggcttgcctc tgacgaaaac atcaacaaca taaacctgta tatcgacctg
     3721 ctagaactgc tgggtgaact ttaccagaaa caaggcaact atagaaaatc agctgaaatg
     3781 tacagcaagg tgctggcaat agttgacaac ccgatgggcc gaaaggtgct tttgaaggaa
     3841 aaaatcaaat ctgacgcctt ctttaagctg ggacaggtaa aaaagaattc gggggaactg
     3901 gtagaaagcc taagcaactt taaacaagct gcagaaattt ctataaataa ttttgattca
     3961 cttagctact ccaatgcact tcaggaaata gcgctcacct actaccttct acaaaaaccc
     4021 gatagcgcca acttttactt tacagcctca tacgccatca gcaacaaaat ttccgacacg
     4081 gtaggcatga ttcaaggcct tcagggaatt gctgatattt acctcgattc aggaaatcct
     4141 actcaagccc tctactacta caaccagcaa ctcaccgtgg cacaaaagtt aaaaaatatt
     4201 cccgctatca ccgaatccct ggtaaaaatt tcgagatgct acctcagcag cggcgatatt
     4261 tccaacgctt ctaaatacct aaatagagcc ttggcaattg cccggcaaaa caacctcacc
     4321 cagtcggtag ccgacgttta ccagtacctg gccatcctaa acgagggtca gggcaggtat
     4381 agggatgccc tcgagttcta taaaatgtgg gttgaaacac gcgactccat ctacttcgag
     4441 gaaactggac agaagctggc taaacttaaa atcctctacg agatttccca gaaagagaag
     4501 gagaatgagc tgctgaggca gaattccgag attcagaacc tgcagcttaa caaaaccagg
     4561 taccaaacgc gaatactgat tctcatcatc atcattttca tcaccctaat tgttctttta
     4621 atcatactct ttcggtctaa gcagaaggaa attgccaaac gccttgaaac ggaacagcgc
     4681 atcaccgatt tgaaccgcga gcttgagcgc aggatgattc aggagataaa aaaacaggaa
     4741 aaacaacagc tactgcttgc ccaaaagtcg aaacttgagt ccctaggtac gcttgcagcg
     4801 ggcattgccc atgaaataaa ccaacccctg ggaggcattt caatggggct cgacaacatc
     4861 ctgctgaagg tgcaggataa aagctacaac gacgagcaca tgaaggaaaa aatagcctca
     4921 ctttttgaaa atgtggagcg tattgaaaaa attattgacc atattcgcta tttttcacga
     4981 gctcaaaagc ctattacctt cacacaaatc aacataaacg atgtggttaa cagctccctt
     5041 ttcatggtat ctacccagta cgagaaccac ggggtaaaaa tggaagttac ccttgacgaa
     5101 aacatcggta gcattaccgc cgacaagtac aagctggagc aggttatcct taacctactt
     5161 tccaacgcca agtatgctgt tgacgaaaaa gggaaaaaac acgccaacca ccagtacaca
     5221 aaactgattg agattaaaac atggcaggat aacaatcagg tttacctaag cgttcgggat
     5281 aacggcatgg gcattcccga caaaatcatc gacaagatat tcgacccatt ttttacaacc
     5341 aaaagcgagg aaaggggaac cgggcttggg ctttcggtat cctatgcatt cattaaggat
     5401 ttactaggtg acattaaagt tgaaagcatt gagggtgaat atacccgctt tgaaatctca
     5461 cttcccaaaa tgtagatagg catgaaggaa ataagaatct tagtgctgga cgacgaaaag
     5521 gtttttcggg atgaaataaa ggaattcctc gaaaacgatg gtttcacggt attgctctcc
     5581 gataggccat cagcagcatt cgagatgctc acccagcagg aagttgacat catgatcctg
     5641 gatataaaac ttcccgaaat ggatggcttt gaggtcctcc ggcgtgttaa ggaggaatac
     5701 cccgacgttg aagtaattat gattaccgga catggcgaca tggaggctgt aatacaggcc
     5761 atgcggctgg gtgcagtaga gtttttcccg aaacctttcc gattactcga catgcgagcg
     5821 gccatccagc gaaccaagcg ctacattacc atcagcaacc aacttaagga aatcaaccag
     5881 acctactcgc ttatttcgaa ggagctgatt gaaagtgtgg gtaacgagat tctgggaaca
     5941 tcacccgcca taagccatgt tgttaacctt atgggtaggg tggccaaaac agaaaatacc
     6001 tcagtgctga ttaccggtga aagcggagct gggaaagaac tggttgcccg tggaattcat
     6061 tatctcagca atagaaagga ctcatacttc tacgcagtaa actgctcggc cattcccgaa
     6121 tcgctttttg aaagtgaatt ttttgggcat aaaaaaggct cctacactgg cgccaacgag
     6181 gacaaagccg gttggttcga ggtagccaac gggggcaccc tctttctgga cgaaatagtt
     6241 gaaatgcttc ctgccatgca gagcaagcta ctcagggtgc tggaagaacg taaggttcgc
     6301 agaattggct caagcgttga tattcctgtt gacgtgcgca taattgctgc caccaaccag
     6361 aacgttttca cacagctcga aaccggcaag ctccgtagcg atctctacta ccggttaaat
     6421 acattcgaaa tccacattcc acctctgagg gaacgtaggg aggatattcc agtacttctg
     6481 gactactatg taaagttttt tgcccgtaag ctgggcaaaa ggatttccga aatagaccc
//
LOCUS       DAOOQX010000021         6430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_14705_length_6430_cov_3.421490, whole genome shotgun sequence.
ACCESSION   DAOOQX010000021 DAOOQX010000000
VERSION     DAOOQX010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14705_length_6430_cov_3.421490"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLX53_00765"
     CDS             <1..358
                     /locus_tag="PLX53_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOY71641.1"
                     /translation="KSQVLKIEHPQAVIPVRVGNRNISDEVVGSVTLFIVFYILILLA
                     STMLLAAMGVDLLTGFSASVTTMGNAGPGFGLVGSMSNFSTIPEPGLVVLSLDMLLGR
                     LEIFGFILLFLVRSWR"
     gene            707..1330
                     /locus_tag="PLX53_00770"
     CDS             707..1330
                     /locus_tag="PLX53_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOY71642.1"
                     /translation="MIVKEQIVNQAMLLFRQMGIRSVTMDMIAEQTGISKRTLYENFS
                     NKDELVEACVMQEIESRKEQAKRIIEGSSHIIETYILFMWDHINRLRGTSPLFIKDIQ
                     KLYPQSICKQTNEFNNRMRENMKQFIIMGIKDGVFRKEINPDIASILIFEQIKVLHVE
                     GGFETSPLSMFSPAEVFEHISINFIRGISTSKGLELVDFYYSKHKMV"
     gene            1357..2721
                     /locus_tag="PLX53_00775"
     CDS             1357..2721
                     /locus_tag="PLX53_00775"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOY71643.1"
                     /translation="MRKTIFHVLYGLGVAMAVSLTPLSGYGQTDTLKLTVKDAVNMAI
                     QQNLMQKVSELELKKKEEKVGEYYAALYPNIKATGSYSRYLELPVIFIPEGTFGPGSG
                     GAMKIGYDNSYSGGFSATLPIFMMNIYQSIRMGKTDVEISAEKMRENQINLTASVKNT
                     FYNLLLLEQSYQVIEMSYQNAQANLENIQKLNASGMVSDYDAIRIKVQVDNLYPTLMQ
                     TKNTYNNLLNIFKVLLNVNDTIPVTLDHSELSTVTLEEIASPTEVWQQDNTTLRQLEL
                     SRKMIGIQEKMVRSSSLPSLVAMGNYQYQAQANDFKFNDYRWVPTSLVGLQLNIPIFS
                     GFAVRKQLNQVKISAQQLDMQMEFTRSNLSAQVQNSVNAINTAIKKVESAQGNVTLAQ
                     KGYAIAQTRYNTGQATLLELNDADNAMMQARLNLIQAQFEYLNARNEYEKITGQSALP
                     TPAN"
     gene            2748..3782
                     /locus_tag="PLX53_00780"
     CDS             2748..3782
                     /locus_tag="PLX53_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOY71644.1"
                     /translation="MKTARTLLILSFSALIASCTGNKTEVTGETKQAEVRVSTQPVEI
                     QKVEHQLRLPATVQAYKENHLAPATAGRVEKVLVDVGDRVRAGQVVAILDRTSYQQAK
                     IQFDKLSIDMARMDSLLKVGAVPQQQYDQVKMQYDIAKTNLQFLDDNTTLKSPIDGVI
                     TGKYINDGEMFAMTPVAGIGKAAIVSIMQLDRLKLLVGVSSSYLSKIELSSSVLITTE
                     VFPGEKFNGKIEKIYPTIDNLTKTFTIEVVVPNRSNMLRPGMFATAFITIGSGNSILI
                     PGYVIQKQSGTNERYVFTYSDGKAVRKVIEVGEVFDDKIEITSGLNPGDELIVVGQAN
                     LMDGQAVTKI"
     gene            3821..>6430
                     /locus_tag="PLX53_00785"
     CDS             3821..>6430
                     /locus_tag="PLX53_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOY71645.1"
                     /translation="MSLYSTAVNKPVSTIMIFIAIIVLGIFSITYIPVDLMPDMDIPM
                     ISVITTYPGANAEDIEINVTKPMENQLNSLQNLKKLTSSSKDNVSYVACEFEYGINMD
                     EASNNVRDAVSLAENALPDDCEKPMILKFNSSMVPVLIYGIKADKSYAALEKIVDERI
                     VERLSRIDGVGTVTIMGKPIRIINVAVDPRKLDSYNLSLEQVGAAIGAENMMLPAGRI
                     EVGKMEFPVRTNGDFSSSDVVKDLVISNNAGKVVYLKDVATVKDTLKKILVDETIDGQ
                     KGVRLMVQKQSGANTVKIAKMVRSRMEEIKTELPSDVQIVKIMDSSEFIQGSINNLSE
                     TLIYAFIFVVLVVILSLGQWRSTFIILTTLPVSLISGFIYLYATGNSINIISLSAMSI
                     AIGMVVDDAIVVLENITKHIERGSTPREASIFATREVGLAVIASTLVIIAVFFPLTLV
                     GGLTGVFFRQLGWIVTITITVSVIAALSLTPMLSSRMLKSKSHYKHKRNSFVKIVNKF
                     WGFFDEIYGRTLKAILNHKTLTLTGAFIIFVGSLFLMKFIGTEFTPSQDNARISVYME
                     VPQGLRIDETKKLARDFEKKIKAKYPEIELISTSIGADEGGSLMSIFQKSASYIINYT
                     LSLSSIDNRTRTIFEIADSMRRDVAAYPQITTSYVDAGGTRNAGGSAMGMGGGNNVEV
                     KILGYDISQTNQIAEEIATTLKNEKGFKDVSISRDQDRMELQVIPDREKLAQLGLNTT
                     MLASAVRNRVYGFTATKYREEGTEYDVIVKYDDDNLTSISDLENITFKGPQGNLVRVS
                     DVAQVRQVYTTPNIDHENKTRIVKVSSSLTGIDLGKATGIINAKLSTMTVPQNVLVEI
                     GGAAQDMMDTFHDL"
BASE COUNT         1930 a         1489 c         1441 g         1570 t
ORIGIN      
        1 caaaagccaa gtgctaaaaa tagagcaccc ccaggctgtt atccccgtta gggtaggcaa
       61 cagaaatatt tctgacgagg tggttggctc agtaaccctg tttattgttt tctacatcct
      121 gattttgctg gcgagcacca tgctgctggc tgcaatgggg gttgacctgc ttacagggtt
      181 ttctgcttcg gtaaccacca tgggtaatgc cggcccaggt tttggactgg ttggatcaat
      241 gtccaacttt tctaccatcc ccgaaccagg ccttgtggtt ctttcacttg acatgctgct
      301 gggaaggctc gaaattttcg gcttcatcct gctttttctg gttaggtcgt ggcggtagcc
      361 tcatatttac cttctaagct ataatggcat tctacacatt ttcggtgcat gatagcaaga
      421 ttctagcagc actttaagga gtagttattc gcccttgtgt agcttacaag agaactaaca
      481 cccttacaac ttttctcgcc tatcggcttg ttgatgtagc tttgccttcc acccgttcga
      541 acaaatactt tatatttaaa ggaagtgtgt gtaattccgc ataaagcata gaggagctgc
      601 tacaaaaaat taaagaatat ttaacaaata ttaaaaaaac ttttattgtt taaaaagttt
      661 tttgtaataa cattgcggtc aaattaaagg agggtaaaac cgaaaaatga tagtaaagga
      721 acaaatagta aaccaggcta tgcttctatt ccggcaaatg ggcataagaa gtgtaaccat
      781 ggatatgatt gccgagcaaa caggaatatc gaaacgtacg ctttacgaga atttctcgaa
      841 caaggacgag ctggttgaag cctgcgtgat gcaggaaatt gaaagccgaa aggaacaagc
      901 caagcgaatt atagagggaa gttcacacat cattgaaacc tacatcctct ttatgtggga
      961 ccatatcaac cggctgcgtg ggacaagccc cttgtttatt aaggacatac aaaaacttta
     1021 ccctcaaagc atctgcaagc agacaaacga attcaacaac cgaatgaggg agaacatgaa
     1081 gcagttcatc atcatgggga ttaaggatgg ggtgttccgc aaggagataa accccgacat
     1141 tgcctccatc ctgatttttg aacagataaa agtgctgcat gttgaaggcg gatttgaaac
     1201 atcgccactt tccatgtttt ctcctgccga agttttcgaa catatttcta taaattttat
     1261 tcggggtatc tcaaccagca aggggcttga actggtagat ttttactact caaaacataa
     1321 aatggtctaa cacttaaaaa tcctaatatg taccttatgc gaaaaacaat ctttcacgtg
     1381 ttatatggat taggagtggc aatggcagta tcgctcactc cattgtccgg gtatgggcaa
     1441 accgataccc ttaagctcac cgtaaaggat gctgtaaaca tggccatcca gcaaaatctg
     1501 atgcaaaagg tgtcggagct ggagctaaag aaaaaggagg aaaaagttgg cgagtactat
     1561 gccgcccttt accccaacat aaaagctacc ggcagctaca gcaggtacct tgaactacct
     1621 gtaattttta ttcctgaggg tacgtttggc cctggaagcg gcggagccat gaaaattggc
     1681 tatgacaaca gctactcggg aggtttttcg gccacccttc ctattttcat gatgaatata
     1741 taccagtcca tccggatggg gaaaaccgat gtggaaatct ctgctgaaaa aatgcgggaa
     1801 aaccagatca acctgacagc tagtgtaaaa aatacgttct acaacctcct gctactggag
     1861 caaagctacc aggttatcga aatgagctac caaaatgccc aggccaacct ggaaaatatt
     1921 caaaaactaa atgccagcgg aatggtttcc gactacgatg ccattcgaat caaagttcag
     1981 gttgacaacc tatatcccac ccttatgcag acaaaaaaca cctataacaa cctgcttaac
     2041 atttttaagg tgctcctcaa cgttaacgac accatccctg taaccctcga ccatagcgag
     2101 ctaagcacgg ttacccttga ggaaattgct tccccaacgg aagtttggca gcaggacaac
     2161 accacactta ggcagctgga gctttcacgt aaaatgattg gcattcagga aaaaatggta
     2221 agaagcagca gcctgccctc gctggtggct atggggaact accagtacca ggcccaggcc
     2281 aacgatttta agtttaacga ttatcgctgg gtacccacat cgcttgttgg acttcagctt
     2341 aatatcccca tttttagcgg gtttgccgtt cgcaagcagc taaatcaggt taaaattagc
     2401 gcccagcagc tcgacatgca gatggagttt acccgcagca acctttccgc ccaggttcaa
     2461 aactccgtta atgcaattaa cactgccata aaaaaagtag aatcggctca ggggaatgtt
     2521 acccttgccc aaaagggtta cgctatagct caaacccgtt acaacaccgg acaggcaacc
     2581 ctgcttgaac tcaacgacgc cgataatgcc atgatgcagg ctaggctcaa ccttatacag
     2641 gctcaattcg agtatctaaa tgcccgaaac gagtacgaaa aaattacagg acaatccgct
     2701 ttaccaacac cagctaacta atcaacaaaa aaaataaact tcagactatg aaaacagcaa
     2761 gaacattact aatactttca ttttcagcgc ttatagcctc gtgcaccgga aacaaaaccg
     2821 aggttaccgg tgaaacaaaa caggccgagg taagagtatc aacacaacct gttgaaattc
     2881 agaaggtaga acaccagctg cgactcccgg caaccgttca agcctataag gaaaatcatc
     2941 tggcacccgc caccgccgga agggtggaaa aagttctggt tgacgtaggt gatagggtaa
     3001 gagccggaca ggtagtggcc attctcgata ggacaagcta ccagcaggct aaaatccagt
     3061 tcgacaagct tagcattgat atggcccgca tggactccct tctgaaggtt ggagcagttc
     3121 ctcaacagca gtacgatcag gttaagatgc agtacgacat cgccaaaacc aacctgcaat
     3181 ttcttgacga taacaccacc ctgaaatcgc ccatagacgg cgttatcacc gggaagtaca
     3241 tcaacgacgg ggaaatgttt gccatgactc cggtagccgg cataggaaag gcagctattg
     3301 tttccatcat gcagctcgac aggcttaagc tgctggttgg cgtttcgtca agctacctaa
     3361 gcaagataga gctaagctcc tcggtactga ttaccaccga ggttttccct ggcgaaaagt
     3421 tcaacggtaa aattgaaaag atatacccca ccattgataa cctcaccaaa acttttacca
     3481 ttgaggtggt tgtacccaac cggtcgaaca tgctccgtcc gggtatgttt gccacggcat
     3541 tcatcaccat tggaagcggc aactctatac taattcccgg atacgttata cagaaacaat
     3601 cgggaaccaa cgagcgctac gtattcacct actcagatgg aaaagctgtt cggaaagtta
     3661 ttgaagtggg tgaagttttc gacgataaaa ttgagattac cagcgggctt aacccaggcg
     3721 atgagctcat agtagttgga caggccaacc tgatggatgg acaagcggta acaaaaattt
     3781 agtaccaacc tttttttatc agctaattaa aacacgcatt atgagtttat atagcacagc
     3841 agttaataaa ccggttagta ctataatgat ttttattgcc attatagtgc tgggaatatt
     3901 ctctatcacc tacattcccg ttgacttgat gcccgatatg gacatcccga tgatcagcgt
     3961 tatcaccacc taccctggcg ctaacgccga agatatcgag atcaacgtta ccaaacccat
     4021 ggagaaccag cttaactctc tccagaatct caagaaacta acctcgtcga gcaaggacaa
     4081 cgtgtcgtat gttgcctgtg agttcgagta tggcataaat atggatgaag ccagcaacaa
     4141 cgttagggat gctgtcagcc ttgccgaaaa tgcgcttcct gacgattgtg aaaaacccat
     4201 gattctgaaa ttcaactcgt ccatggtgcc ggtgctcatc tacgggatta aagccgacaa
     4261 gagctacgca gcgctggaga aaatagttga cgaaagaata gttgaacggc taagccgaat
     4321 tgacggggtg ggaacagtaa ccattatggg taagcccata agaattatca atgttgcggt
     4381 tgatccccgt aagctcgact cctacaacct cagcctcgaa caggttggag cagccattgg
     4441 agccgaaaac atgatgcttc ctgccggacg tattgaagtt ggaaaaatgg agtttcctgt
     4501 taggaccaat ggcgatttca gcagcagcga tgtggtaaag gacctggtta tctccaacaa
     4561 tgccggaaag gttgtttacc ttaaagatgt ggccactgtt aaggataccc taaagaaaat
     4621 tctggttgac gaaacaatag acggccagaa gggtgtcagg ctaatggttc agaagcaaag
     4681 cggcgccaac acggtaaaaa ttgccaagat ggttcgcagc cgcatggagg agattaaaac
     4741 ggaactgcca tctgacgtcc agatagttaa aatcatggac tcatccgaat tcatccaggg
     4801 ctctattaac aacctttccg aaaccctaat ttatgccttt atatttgtag ttctcgttgt
     4861 aatcctgtcg cttgggcagt ggcgctcaac ctttattatt ctgaccacac tccccgtttc
     4921 gctgataagc ggtttcatat acctttacgc caccggcaac tccatcaaca taatctcact
     4981 atcagccatg tctattgcaa ttgggatggt agtggacgac gccattgtgg tgcttgaaaa
     5041 cattaccaag catattgaac ggggaagtac accgagggag gcatctatat ttgcaacacg
     5101 tgaagttgga cttgccgtta tcgcctccac cctggttatt atagcggttt tcttccctct
     5161 gacgctagtt ggcgggctta caggtgtttt ctttagacag ctgggatgga ttgtaactat
     5221 taccatcacc gtttcggtaa tcgctgcgct ttcccttacc ccaatgctct cgtcaagaat
     5281 gcttaagagc aaaagccact acaagcacaa acgcaacagc tttgtcaaaa ttgtaaacaa
     5341 attttgggga ttctttgacg agatatacgg tcgcaccctg aaagctattt tgaaccacaa
     5401 gactctaacc ctgactggtg ctttcataat atttgtgggt agtctattcc taatgaagtt
     5461 catcggcacc gagttcaccc cctcacagga taacgcccga ataagcgttt acatggaggt
     5521 tccccagggg ttgagaattg acgaaacaaa gaagctggca cgcgattttg agaaaaagat
     5581 aaaggcaaaa tatcctgaaa ttgagcttat ttcaaccagc ataggtgccg acgagggggg
     5641 atcgctgatg agcatcttcc agaaatcagc ctcgtacatc atcaactata cgttgagctt
     5701 gagcagcatt gataaccgaa cgcggacaat ctttgaaatt gccgatagca tgaggcgtga
     5761 tgttgccgcc tacccacaga ttaccacctc atacgtggat gcaggtggca cccgaaacgc
     5821 aggtggctca gcaatgggta tgggcggtgg caataatgtt gaggttaaaa tacttggcta
     5881 cgacattagc caaaccaacc agattgccga agaaattgcc actacgctta aaaatgagaa
     5941 ggggttcaag gatgtttcca taagccgtga ccaggatcgt atggagcttc aggttattcc
     6001 cgatcgagaa aaacttgccc agcttgggct gaacactaca atgctggcca gcgccgtccg
     6061 taacagggtt tatggtttta ccgcaaccaa ataccgtgag gagggaacag agtacgatgt
     6121 aattgttaag tacgacgatg ataacctaac ctcaataagc gacctggaaa acattacctt
     6181 taagggacct cagggaaacc tggtaagggt aagcgatgtg gcgcaagtta gacaggttta
     6241 cactaccccc aacattgacc acgagaacaa gacgagaatt gtaaaggttt ccagctcact
     6301 taccggaatt gacctgggga aagctacggg cattataaac gccaaacttt ccaccatgac
     6361 cgttccccaa aatgtgctgg ttgaaattgg cggagcagca caggacatga tggatacatt
     6421 ccacgattta
//
LOCUS       DAOOQX010000022         6268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_15164_length_6268_cov_4.001770, whole genome shotgun sequence.
ACCESSION   DAOOQX010000022 DAOOQX010000000
VERSION     DAOOQX010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15164_length_6268_cov_4.001770"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLX53_00790"
     CDS             <1..918
                     /locus_tag="PLX53_00790"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOY71646.1"
                     /translation="PTLWFRYVNFNLNQWATWNFGGQSTDKGGNINFNLQFTNYWTFN
                     AGINYNSESLSAHALRGGPMMKIPGSWNAWYNIGTDGRKKLVFYMGGSTLESNENYAY
                     YQSVYATLSYRPLKNLQLSLNPSYSVNSDKLEFVENVDYGNETRYIQGKLSQKTFALS
                     LRVEFSINPDLSVQYYGRPYLSSGKYTKFKRITNPMANSFDQRFVTFEPSQLVYNQDE
                     EEYYVDENNDGGSDYSFSNRNFNFMDLQSNLIVRWEYRPGSTLFLVWTLGKQAYESEY
                     GSSLNNNIETLYDAHPHNIFLLKFSYRFN"
     gene            1239..1787
                     /locus_tag="PLX53_00795"
     CDS             1239..1787
                     /locus_tag="PLX53_00795"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOY71647.1"
                     /translation="MKIYTKTGDKGMTSLIGGMRVPKYHPRIDTYGTVDELIAFIGLL
                     RDQPVDGHTRDMLVFVQDKLMSCAAVLAAEEPNSKTDLPTVSDADVRKLEADIDAMDA
                     LLEPLTSFVLPGGHPTVSLCHVCRTICRRAERLAVKLATETPLPVNLTIFLNRLSDYL
                     FTLSRKLAKDLKVDQIKWHPDK"
     gene            1873..2094
                     /locus_tag="PLX53_00800"
     CDS             1873..2094
                     /locus_tag="PLX53_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOY71648.1"
                     /translation="MYWTLELASKLEDAPWPATKEELIDYAIRSGAPLEVIENLQELE
                     EETEIYESIEDIWPDYPTKEDFFFNEDEY"
     gene            complement(2315..5416)
                     /locus_tag="PLX53_00805"
     CDS             complement(2315..5416)
                     /locus_tag="PLX53_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY71649.1"
                     /translation="MSESILKALMQLFAIISRPVAEEEMEANDYSDRRRVVEEFLRNQ
                     LNQELVAEYIKVFDNYYNLYQEKQSEKTKSKKRTSSSSVRVLKICAQINEELQQQQKI
                     VVLVRLLEFVKPEEGKVTEQEMAFISTVAETFYVSADEFNMLSDFVLSSFNQIPITPN
                     VLLIDGNKDFELPGVKHIYAEGLLDELRVVFIESANIFFLRYRGTHELYLNGHLIQNE
                     KIFVLSNGSSIRNPKIKPVYFSDIVGRFALDKLKERIVFEALDVEYRFRSGKIGIHQM
                     TFTEESGHLVGIMGASGAGKSTLLNVLNGSEKPTAGNVLINGIDIHRQPELVEGQIGF
                     VSQDDLLIEELTVYQNLYFNARLCFDNYTPQQLDEAVSRTLVNLGLNEIRDMRVGTPL
                     NKKISGGQRKRLNIALELIREPAILFLDEPTSGLSSRDSENILDLLKELTLKGKLVFV
                     VIHQPSSEIFKMLDKLLILDTGGYLIYNGDPIESIMYFKSRVQHANWNESECATCGNV
                     NPEQIFNIVEANVLDEYGNPTRARKISPVEWNNHYKKWFSAQPQLDGIPDFLPKIHFK
                     TPGWFKQLKIFVKRDVLSKLANSQYMIINVLETPLLAFLLSYIIKYFNIDVSNRVGYT
                     FMENSNIPVYLFMSVIVGVFVGLTVSAEEIIKDRKIQKRESFLNLSWSSYLMSKVAIL
                     LVLSAVQAFMFVIVGNSILEVRGMYFEYWLVLFSAWFSSNMLGLVISDSFKTVVTIYI
                     LIPFLVIPQIILSGVIVKYEKLNPKISSPSSIPFYGELIVARWAYEALAVEQFCNNPY
                     EKPFYVFDEAMSISDYKRNYWLKSLQNKVDFCSRNLGLPEKSEEVEDALTLLRNEIMH
                     EMTSPQGSKLTFSKYNQVSMAGVSPELLDELKSYLELVRKYYVKLYNKASSQKDAYIS
                     SFQAAPGGQEAFVNLKRQYYNESLTDFVRNTGEVERITEYKGRLIQKIDPIFLYPDTK
                     FVKAHFYSPVKPVFGKFAGTFWVNVAIIWISNILLYLILYYRLLKRFLEQFDRIGAEK
                     EAN"
     gene            complement(5439..5855)
                     /locus_tag="PLX53_00810"
     CDS             complement(5439..5855)
                     /locus_tag="PLX53_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71650.1"
                     /translation="MAKKILIPFILIGLFSVKATAQVENLRNTCALNFPPEYVSDGQE
                     YFASLKPDQVIEFRTTFFGENTYRITTCTSVKKGQVVLTVYDTDKNLLFSNRDYDYSP
                     YWNFRFTSTVICIIQVDVVSQQFTPGYVMLLIGFKQ"
     gene            complement(5868..>6268)
                     /locus_tag="PLX53_00815"
     CDS             complement(5868..>6268)
                     /locus_tag="PLX53_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71651.1"
                     /translation="YLKTNERSDIGALIVAGSWIESMYFLTQIALGNSNRDIINRFGE
                     QKHPLDNLIELLTPYYYKSSDFSELIDELINVAYEYDGIIYSYKYKEPIIDAANRQIT
                     INSTTQVVVSEYHIRIISSKIESIRNKLVE"
BASE COUNT         1746 a         1447 c         1387 g         1688 t
ORIGIN      
        1 cctaccctgt ggtttcgcta tgtaaacttt aaccttaacc agtgggctac atggaacttt
       61 ggagggcagt caaccgataa aggtggaaat attaacttca acctacagtt caccaactac
      121 tggacattta atgccgggat taactataac agcgagtctc tgagcgccca tgcccttcgt
      181 ggtggtccaa tgatgaagat accaggtagc tggaatgcat ggtataatat agggactgat
      241 gggagaaaaa agctggtttt ctacatgggt ggatcaacgc tggaaagcaa tgagaactac
      301 gcttactacc aatcggttta cgcaaccctt tcataccgcc cccttaaaaa tctgcaactt
      361 tctctaaacc cgtcatactc cgtaaacagt gataagctcg aatttgttga aaatgttgat
      421 tacggcaacg aaacccgata tatccaggga aaacttagcc agaaaacgtt tgccctttcg
      481 ttgagagtgg aatttagcat taaccccgac ctgtcggtgc agtactatgg ccgaccctac
      541 ctttcaagtg gcaagtacac caaatttaag cgtattacca atccaatggc caacagtttc
      601 gatcaacgat tcgtaacctt tgagcccagc cagcttgttt acaaccagga tgaggaggaa
      661 tactatgttg acgagaacaa cgatggcggt tccgactact cattctccaa ccgcaacttc
      721 aactttatgg acctacagtc gaacctgatt gtgcgctggg agtacagacc ggggtcaacc
      781 cttttcctgg tttggacgct gggtaagcaa gcctacgaat cggagtacgg cagctcgttg
      841 aacaacaaca tcgaaaccct ttacgacgct cacccccata atatattcct cctgaaattt
      901 tcgtacagat tcaactagcc ctaaacactc ggcattcgaa tgtaggcagg aagcacctgc
      961 aacgctccac ctgtagatgt taatcccaac ccgcattatt cccatcagca acttgaggaa
     1021 tcatctgaac acatgtcagc aattgcctac cgcaatactt catagaagtt cacagatccg
     1081 gccttcaaaa aacctgcagc atcacacccg tgaaaaaatt caaaaccgac atttaaaaat
     1141 ttataaaaat ttaatttcaa aatactaaca aatctaaacc aaaacatgta tgtttagggt
     1201 gaagtttgca ctacgattat ttcacagcta gccataccat gaaaatatat accaaaacgg
     1261 gcgacaaggg gatgacctcc ctgataggcg gaatgcgtgt tcccaagtac caccccagga
     1321 tagataccta cggaacagtt gatgagctaa ttgccttcat cgggttgctg cgcgatcaac
     1381 cagttgatgg ccacaccagg gatatgctgg tatttgtcca ggataaattg atgagctgtg
     1441 ccgcagtgct tgcagccgaa gaacccaact caaaaactga cttaccaaca gtttccgatg
     1501 ccgacgttag gaagttggaa gcagacattg atgccatgga tgcccttctt gaacccctga
     1561 catcgttcgt tcttcccggt ggacatccta cagtttcgct ttgccatgtt tgcagaacaa
     1621 tttgccgtag ggctgaacga ctggccgtta aacttgcaac cgaaacgccc ctacccgtca
     1681 acctgactat atttctgaat cggcttagcg attacctttt cacgctttcc cggaagctgg
     1741 caaaagattt aaaggtagat cagataaaat ggcatcctga taagtaattc caatttttta
     1801 tatatttgcg ggtatatggt tatttataat ctattaatta tcagtttatt aactaaaata
     1861 taaattctac ctatgtactg gacacttgaa ttagcatcaa agttggaaga tgcgccctgg
     1921 ccggcaacaa aggaggaact cattgactat gcaattcgct caggcgcacc acttgaggtg
     1981 attgaaaacc tgcaagaact tgaggaggaa actgaaatat atgaaagcat agaggatatt
     2041 tggcccgatt atcccaccaa ggaggatttc ttttttaacg aggatgaata ctaatcattc
     2101 taaatggtaa caaagcaacc agcaagcaac cattcaaaag aaattagaaa aagccacttc
     2161 ggaatccatg taggtgaact ctctgccccc gccatgaagg gttatttgcc ttacagccaa
     2221 aaagtttttg gtaggggtag catgtttttg cataacccac ctgtggaaaa ctgtggaata
     2281 ataggaaaga aggattgttg gtgttgaccc ggctttagtt cgcttccttc tcggcgccaa
     2341 ttctatcaaa ctgctctaaa aaacgtttaa gcaacctata gtataaaata aggtagagca
     2401 ggatgtttga aatccaaata attgccacat ttacccagaa agttccagca aattttccaa
     2461 ataccggctt aacaggtgag tagaagtgtg ccttcacaaa ctttgtatca ggataaagga
     2521 aaatgggatc aattttctga ataagccttc ctttatactc ggtaattctt tccacttcac
     2581 ccgtgtttct gacaaaatct gtaagactct cgttgtagta ttgccgttta aggtttacaa
     2641 atgcttcctg cccaccagga gcagcctgaa agctggatat gtaggcatcc ttctggctac
     2701 tggctttgtt ataaagcttc acgtagtact tgcgaaccag ctccaggtag cttttcagct
     2761 cgtcgagcaa ctcgggtgaa actccagcca tggaaacctg gttatactta ctaaaagtaa
     2821 gcttcgagcc ctgcggcgat gtcatttcat gcataatttc gttacgcagc agggttaggg
     2881 catcttccac ctcctcactt ttttcgggca agcccaggtt ccgagagcag aagtcaacct
     2941 tattctgaag cgattttaac cagtagttcc tcttgtagtc cgaaatgctc atggcctcat
     3001 caaacacgta gaagggtttc tcatacgggt tattacagaa ctgctcaact gccagtgcct
     3061 cgtaggccca tcgggcaaca atcagctccc cgtaaaatgg aatgctactt ggtgaggata
     3121 ttttgggatt aagcttttcg tactttacaa ttaccccgct caagatgatc tggggaataa
     3181 ccaaaaacgg gatgagaata taaattgtta caaccgtctt gaagctatcc gagatgacca
     3241 accccagcat gttcgacgag aaccatgcgc tgaatagaac cagccagtac tcgaagtaca
     3301 tcccgcgaac ctcaaggatt gaattcccaa caattacaaa catgaatgcc tgcactgccg
     3361 acaacaccag cagtatggca accttcgaca tcaggtagct ggaccagctg aggttcagga
     3421 atgattcgcg tttctgaatt tttctatcct taataatctc ctcggcgctt accgtgaggc
     3481 ctacgaaaac tcccacaatc accgacatga acaggtaaac aggaatgttg ctgttttcca
     3541 tgaaggtgta gccaaccctg tttgaaacat caatgttgaa gtacttgata atgtaggaca
     3601 gtaggaaggc caacagcggt gtttctagca cattgataat catgtactgc gagttggcca
     3661 gcttggacag cacgtcgcgc ttgacaaaaa ttttcagctg cttaaaccaa ccaggggtct
     3721 taaagtgtat tttgggaagg aaatcgggaa taccgtctag ctggggttga gctgaaaacc
     3781 actttttgta gtggttgttc cactctacgg ggcttatttt acgggcacgg gtaggattgc
     3841 cgtactcgtc gagcacgttg gcctccacaa tgttgaatat ttgttcgggg ttcacattgc
     3901 cacaggtggc gcattcgctc tcgttccagt tggcatgctg aacccttgac ttaaagtaca
     3961 tgatggactc gataggatcg ccattgtaga tgaggtagcc acccgtgtcg agtatcagga
     4021 gcttgtcgag catcttgaat atttcggacg agggttgatg gattaccacg aaaacaagtt
     4081 ttcccttcag ggtaagttcc ttgagcaggt cgaggatgtt ttccgaatcg cgtgagctga
     4141 gaccggaggt tggctcgtcg aggaaaagga tggcaggctc ccgaataagc tccagggcaa
     4201 tgttgagcct ttttcgctga cctccgctta tctttttgtt aaggggtgta cccacccgca
     4261 tatccctgat ttcattaagc ccaaggttta ccagtgttcg ggaaaccgcc tcatcgagct
     4321 gctggggcgt gtagttgtcg aaacacaagc gggcgttgaa atacagattt tggtaaacgg
     4381 taagctcctc aatgagcaga tcgtcctgtg aaacgaatcc aatttgtccc tccaccaact
     4441 cgggttgcct atgaatgtca atgccgttga tgagcacatt tccggctgtg ggcttttcgc
     4501 ttccgttcag cacgttcagc aaggtagatt ttcctgcacc cgaggctccc ataatgccta
     4561 ccaggtgtcc cgattcctca gtaaacgtca tttggtgaat accgattttt ccacttctga
     4621 atcggtactc aacgtctaaa gcctcgaata ctatcctctc cttgagctta tcgagggcaa
     4681 accgcccaac aatatcgctg aagtaaaccg gtttgatttt aggattgcga atggaagagc
     4741 cattgctcag aacaaaaatt ttctcgttct ggattaggtg gccgtttagg tagagctcat
     4801 gggtgcccct gtaacgcagg aaaaatatgt tggccgactc aataaaaaca acccgcagct
     4861 cgtctagcaa gccctcagcg taaatgtgct tcacaccagg cagctcaaaa tccttgttgc
     4921 cgtcgatgag taaaacattt ggggtaatag gaatttggtt gaagctggag agaacgaaat
     4981 cggaaagcat gttgaactca tcagcggaaa cgtagaatgt ttcagcaacg gtagaaataa
     5041 atgccatttc ctgctcggta accttgccct cttcgggctt aacaaactcc agcagcctaa
     5101 ccagcaccac tattttttgc tgctgctgta gctcttcgtt tatctgggca caaattttca
     5161 gcacccttac cgagcttgac gatgttcttt ttttggactt ggtcttctcg ctttgcttct
     5221 cctggtaaag attgtagtag ttgtcgaaaa ccttgatgta ctcggccacc agctcctggt
     5281 tcagctggtt acgaaggaat tcctccacta cccgacgacg gtcgctgtaa tcattggctt
     5341 ccatttcctc ctctgcaacc ggtcgggaaa tgatggcaaa aagctgcatt agagctttta
     5401 gaatcgattc gctcatggag tgctatataa ggttagaact actgtttaaa cccaataagc
     5461 agcataacat atccaggtgt aaattgctgg cttaccacat ccacctgaat aatacatata
     5521 accgttgaag tgaagcggaa attccagtat ggtgaatagt cataatccct gttggagaaa
     5581 agaaggtttt tatcggtgtc gtacacagtt agaacaacct gccccttttt aacgcttgtg
     5641 caggtggtaa tcctgtaggt attttctcca aagaaagtgg tgcggaactc gataacctgg
     5701 tcgggcttaa gggaggcaaa gtactcctga ccatcggaaa cgtattcggg aggaaagttc
     5761 agtgcacagg tgtttctgag attttctacc tgggcggtgg ctttaactga gaaaagtccg
     5821 attagtatga aggggattaa aattttttta gccatggcaa catattgtca ttcaactaat
     5881 ttattgcgga tactttcaat tttgctgctt atgatccgga tatggtactc cgaaaccacc
     5941 acctgcgtgg tgctatttat tgtgatttgc ctgttggcag catcaataat cggctccttg
     6001 tatttgtagg agtaaattat gccatcgtat tcgtaggcaa cattgataag ctcgtctatc
     6061 agctcggaaa aatcggacga tttgtagtag tagggagtta acagctcaat aagattatcc
     6121 agggggtgtt tctgctcgcc aaaccggtta atgatgtccc ggttgctgtt tccaagagca
     6181 atctgggtca gaaagtacat gctctcaatc cacgaaccgg caactattaa ggcgccaata
     6241 tcgctccgct cgttggtctt aaggtata
//
LOCUS       DAOOQX010000023         6200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_15403_length_6200_cov_3.497640, whole genome shotgun sequence.
ACCESSION   DAOOQX010000023 DAOOQX010000000
VERSION     DAOOQX010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15403_length_6200_cov_3.497640"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1879)
                     /locus_tag="PLX53_00820"
     CDS             complement(<1..1879)
                     /locus_tag="PLX53_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71652.1"
                     /translation="MNKRLVAIVVLAIASLALLLVSQYLSKSALDVRLYRPNVKHLER
                     IVERETTYSGKLAREFLGKVNSNASSRQIFESGVEFKRKLEGKWDGELFLFSHDTLCF
                     WSCKLDVSGVPFDSSQVGLVKIQSEYFFTQWFTAGKGLQLLVLNPIYSEYPYQNKYLK
                     NSFSPLFKEFSNYRISIDLVSNAIPVRPHNVAPFYLVPLDIYQNGHVNRLWPILQWIA
                     FAMAVCAVLLIFGQKFFRYKPLLKIAGATAGIFIIRYLSLYMEIPSRSGMKIFNPEIY
                     AHSFINPSLGDFFINAILVFMLVLFIRQSIRLSLRHIKNMHLYLLAASVLILFLALFP
                     ITDKLLTSLVTHSTLTLEPYRIFSLSVYSIIGYLSVSLWAASAVILTITWVSVVAEQL
                     SLKQRLILLVSGLLVVTLLSLALTSKPSGLGLVWMWLTVAYVLLVYRSSRTHLLLRHI
                     LVIVTIFSAYSVSTVFIKTSEKELNVRKVLAINLSSERDPLAEMLLPNLYQKMLTDSY
                     IRDCIRNIEQKNAELYSYLRKDYFKNYLNRYDLRATVCSPSSIINVDGKSTLLCDRFF
                     GMLIDDYGIMLPSSRFYYLNTQSGFISYVGEINYRIGDNIRNLYIELTSRPGWEMLGY
                     PE"
     gene            complement(1943..2416)
                     /gene="rlmH"
                     /locus_tag="PLX53_00825"
     CDS             complement(1943..2416)
                     /gene="rlmH"
                     /locus_tag="PLX53_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOY71653.1"
                     /translation="MKIHLILIGKTSVPYLSEAMNDYLKRIERYCRFEVKVLPELRNT
                     KSMPVEVQLQKEAEVLMPVMGNYQEVILLDEKGRELSSEEFAGFIQKKQNSGLRDIAF
                     VVGGPYGFSDGVKLAAAERLSLSRMTFSHQLVRLVFVEQLYRAFTIINGEPYHHA"
     gene            2874..3299
                     /locus_tag="PLX53_00830"
     CDS             2874..3299
                     /locus_tag="PLX53_00830"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOY71654.1"
                     /translation="MGKQKIKLLSLFLLIMLGSSASVLQQDSNEIISKSAEALQNGDA
                     VNLAKYFYKTIEIDMLGEENFYSQAQSVQLLKNFFEKNKPVKFAVSHQGAKETSAFAI
                     GQLQTKGNTFRISIFLKSDGSQFFIHQLRIEQDEEGNGI"
     gene            3300..4151
                     /gene="nadC"
                     /locus_tag="PLX53_00835"
     CDS             3300..4151
                     /gene="nadC"
                     /locus_tag="PLX53_00835"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOY71655.1"
                     /translation="MATNPELLVEALLNLAIAEDIGDGDHSTLSCIPPSATGKVQLMM
                     KQEGIIAGLSVALKIFKKIDSSVEVEFFRGDGDLLRYGDIAFKAEGKVHSLLQAERLV
                     LNVLQRMSGVATQTREYVNRCEGTKAKILDTRKTTPGMRILDKMAVVIGGGMNHRMGL
                     FDMIMLKDNHIDFAGGIENAVALAHKYLNETGRKLAVEVEARSLKDVQVILRVGGVNR
                     IMLDNFSVEATREAVGMIGGRVETESSGGITLENIRDYALCGVDFISVGALTHQIRSI
                     DMNLKKI"
     gene            4322..5209
                     /locus_tag="PLX53_00840"
     CDS             4322..5209
                     /locus_tag="PLX53_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71656.1"
                     /translation="MNTKRLLLISAILFTTILAWILPVEAQNRPPQNVYRVTVTSRYE
                     IKDGKRTTNSRAIRQEIKDSLGRTHTILNRDYESQRVTSHTWHTFSGKIITRTDEFEN
                     ERLTRFHLFTYTPDSLIASEAIYRVQPGDTSLYLNIRYGYTNRRPVQAIATSATGKKA
                     YTLKSEWDSQGTEIKRTVKSKKGFAPLDSIALLTCHPTYDSLGRKVAETISRKFLDGS
                     SKTQQFRYAYDSKGNRIMIEELNPSGSTIARTEMQYNDKGMLKFISRFDAQNNLIDYQ
                     AIRYELYPSRNRLNMIIEY"
     gene            complement(5350..>6200)
                     /locus_tag="PLX53_00845"
     CDS             complement(5350..>6200)
                     /locus_tag="PLX53_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOY71657.1"
                     /translation="IGLIIILLMFAAILFWANNQKQKANKLLSEKNAEIFAQKEEISR
                     QADELRELNALKDRLFSIIAHDLRSPLFSLITMLNIAKEGHFTEESFKEIISELSSNV
                     SHTTALLENLLTWAKNQMQGTKVKPERFFLNEVVTPRLQQLNEVALQKNIVITNHVDE
                     STSVYADRDMLDLIIRNLISNAIKFCNAGDKIQIWSSVENSHVKVCVEDTGVGIDAHS
                     LKKLFGSQVSSTPGTRNEKGTGLGLILCREFVEMNNGKIWAESTPGKGSQFFFTLPIS
                     GEQASR"
BASE COUNT         1857 a         1377 c         1347 g         1619 t
ORIGIN      
        1 actctggata gcccagcatc tcccatcccg gtctgctggt aagttcaatg tatagattac
       61 gaatgttgtc acctattcgg tagttaattt ccccaacata gcttataaac ccgctttgcg
      121 tgttcaggta gtaaaatcgt gatgacggta gcataatgcc gtaatcatca atcagcatcc
      181 caaaaaacct gtcgcaaagc aaggtggatt ttccatcaac atttataatt gatgatggcg
      241 aacaaacagt tgcccgcagg tcataccggt tcaggtagtt cttaaagtag tccttacgta
      301 ggtagctgta gagctcggca tttttctgct caatattcct aatgcaatcc cggatgtaag
      361 aatcggtaag cattttttgg tagaggttgg gtaaaagcat ttcggctagc ggatcacgct
      421 ccgagctaag gtttatggca agaactttac gcacattcag ctctttttcc gatgttttga
      481 taaatacggt tgatacggaa taagctgaaa atatggtaac aatcaccaaa atgtgtctta
      541 gtaagagatg ggttcttgag ctgcggtaaa cgaggagaac gtatgcgacc gtcaaccaca
      601 tccaaaccaa ccccaaaccg gatggtttgc tggttagtgc cagcgatagg agcgttacca
      661 ctaagagtcc tgataccagt aggattagcc tttgctttaa gctgagctgt tccgcaacta
      721 cgctaaccca ggtaatggta agtatgaccg cagaggcagc ccaaaggcta acagataggt
      781 atccaattat tgagtaaacc gaaaggctaa atatacggta gggttcaagg gttaaggttg
      841 aatgggtaac cagcgatgta agcagcttgt ccgttatggg gaatagagct aaaaaaagaa
      901 tcagcacaga agctgcaagc aggtataggt gcatgttctt gatatgcctc aatgacaatc
      961 gaatactttg tcttatgaag agaacaagca taaagactag aatagcattt ataaaaaaat
     1021 cgccaagaga tgggttgatg aatgaatggg cgtagatttc ggggttaaat attttcattc
     1081 ccgacctgga gggaatttcc atgtaaaggg acaggtatcg gattataaaa ataccggccg
     1141 ttgccccggc tatcttcaac aatggcttat acctaaaaaa cttttgtccg aagatgagca
     1201 gtactgcgca aactgccatt gcaaaagcta tccactggag aatgggccat aaacgattca
     1261 catggccatt ttggtaaatg tccagaggga caaggtaaaa gggtgcaacg ttatggggtc
     1321 ttacgggtat tgcgttggaa accaggtcaa tggaaattct ataattacta aattccttaa
     1381 acaacgggct aaaggagttt ttcaagtact tgttttggta gggatattcg ctataaattg
     1441 ggtttaacac aagcagctgc agccccttac ctgctgtaaa ccattgcgtg aagaagtact
     1501 cgctttgaat ttttaccaga cctacctgtg aggaatcaaa cggaacgcca gagacatcca
     1561 gcttgcacga ccaaaagcag agcgtatcgt gagaaaaaag gaatagctct ccatcccact
     1621 taccctcaag cttcctctta aattcaactc ccgattcgaa tatttgtctg gagctggcgt
     1681 tgctattaac cttacccaga aactctcggg caagcttgcc ggaataggtt gtttcccttt
     1741 caactattct ctccaaatgc ttaacgttgg gcctatacaa ccttacatca agcgcagact
     1801 tcgacaggta ttgggagacc agcaaaagtg caagcgaagc aatggcaagg acgacaatgg
     1861 caaccagcct cttgttcatt tcgatgagag ttcgggatgg attaggtatg tagctaaatt
     1921 catatcaaaa atcaggccta aatcaggcgt gatggtaagg ttcaccatta ataatggtaa
     1981 aagcacgata gagctgctcc acgaatacca accgaacaag ctggtgcgaa aatgtcattc
     2041 ttgatagcga taacctttcg gcagcagcca gcttcacccc atctgaaaaa ccgtaagggc
     2101 cgccaaccac aaaagcaata tcacgcaaac ccgaattttg cttcttttga ataaaaccgg
     2161 caaactcttc ggatgataac tctctaccct tctcatcaag cagaataacc tcctggtagt
     2221 tccccattac cggcataagc acttctgctt ccttttgtag ctgaacttcc accggcattg
     2281 atttggtatt acgtagctcg ggcaaaactt taacttcgaa gcggcagtac ctttctatcc
     2341 gctttaggta atcgttcatt gcttcggaaa gatacggtac gctggttttc cctataagaa
     2401 tcaagtgaat tttcacaaaa aaaatggttg aagtgattta tccagctttt aaatacgtaa
     2461 tgcttaactc ggtgaaattt ataaaaacat caaccttaat caagtactat caaaccttct
     2521 aaaaaatggg aacatcctgc cggtacttta acccagcaaa aacacctatt tcaaatttta
     2581 ctactaacgc aaaggtaaaa aatacttaca cccaaagctg caccatggag taaacaaaaa
     2641 attcaaaaaa ataatttacg gcttgaagag tgtcggacat tgactcataa aagcttccgt
     2701 actgatggag ataattggaa acaaacatgt tctacaacga cacttggttc aaaaaatgat
     2761 tataggtata tttggcctta ggtataaaga ataaattaat ctataggtta ttggctcaat
     2821 ttttgaggat ttttgtagtt tagccgtgtg gtaacaataa gaaaactgta gaaatgggga
     2881 aacaaaaaat taagctgctt tctctgttcc tgttgatcat gctgggttcg agtgcctctg
     2941 tgcttcaaca ggactccaac gaaattatta gtaaatcagc agaagccttg caaaatggcg
     3001 atgcggttaa tctggcaaaa tatttctaca agaccataga aattgacatg ctgggtgagg
     3061 aaaactttta cagtcaggcg caatccgttc agctgctcaa gaatttcttc gagaaaaaca
     3121 aacccgttaa gtttgccgtt agccatcaag gagcaaagga aacctcggct ttcgccattg
     3181 ggcagctgca gaccaaaggc aatacatttc gcatctccat cttcttaaaa tccgatggta
     3241 gccaattttt cattcatcag ctccgcattg agcaggacga ggaaggaaac ggaatataaa
     3301 tggctacaaa ccctgaactt ctcgttgaag cccttctcaa cctggcaatt gctgaggata
     3361 taggtgatgg cgatcactcc acgctttcgt gtattccccc gtcggccacc ggcaaagtac
     3421 agctaatgat gaaacaggag ggcattattg ccggactatc ggttgctctg aaaattttca
     3481 agaagattga cagctcggtt gaagtagaat tttttagagg cgatggtgat ttgctcagat
     3541 atggagatat cgcctttaag gcggaaggaa aagttcactc cctgttacag gccgaacgct
     3601 tggttctaaa cgtccttcag cgaatgagcg gtgttgctac ccaaacacgg gagtacgtta
     3661 accgctgcga gggaacaaaa gcaaaaatcc tcgacacacg caaaacaaca ccgggcatga
     3721 ggattttgga taaaatggca gtagtaattg gtggagggat gaaccaccgc atgggccttt
     3781 tcgatatgat catgctaaag gataaccata ttgattttgc tgggggaata gaaaatgccg
     3841 ttgcgttggc ccataaatac ctgaatgaaa ctggccgaaa gctggcagtt gaggttgaag
     3901 cgcgctccct caaggatgta caggtcattt tacgggtagg cggggtaaac cggattatgc
     3961 tcgataactt cagcgttgag gccacacgcg aagcagtagg gatgataggt ggcagggtgg
     4021 aaaccgaatc gtccggagga attacgctgg aaaatatcag agactacgcc ctttgcggcg
     4081 ttgactttat atctgttgga gcacttaccc accagataag gagtattgat atgaacctca
     4141 aaaaaatctg aatcgctgct tccgctttac gtgtaatagc tgagcaatct ttttcgcaac
     4201 cctccaaccc cacacgtttt ctacttaagc atcagctaaa ggaaaagctt gaacttgaac
     4261 atttttcgct tttaactcgt atttttacaa ggaaaatttc tactgcctaa cttctataga
     4321 tatgaataca aaacggctac tgctgatttc agccatatta tttacaacca ttctggcgtg
     4381 gatattacct gttgaagccc aaaatcgtcc cccgcaaaat gtgtacaggg ttaccgttac
     4441 cagccgttac gagattaaag atggtaagcg caccaccaat agcagggcta tacggcaaga
     4501 aataaaggac tcgcttggaa gaacccatac aattcttaat cgcgattacg aaagtcagcg
     4561 cgttaccagc catacatggc atacattttc cggaaaaata attacccgta ccgacgaatt
     4621 cgagaacgaa cggttaacca ggtttcacct ttttacctac actcccgact cgcttattgc
     4681 cagtgaggct atttacaggg ttcaacccgg cgatacctcg ctttacctca acatcaggta
     4741 tggctacacc aataggaggc ctgttcaggc cattgccacc tccgctaccg gtaaaaaagc
     4801 ctacacccta aaatcggaat gggacagtca aggaaccgag atcaagcgaa cggtaaaatc
     4861 gaagaaaggt tttgccccac tggattcaat cgcccttctc acctgccacc caacctatga
     4921 ttccctggga agaaaagtgg ccgaaacaat atccagaaaa tttctggatg ggagttctaa
     4981 aacccagcaa ttccgctacg cttatgactc caaaggtaat aggattatga ttgaggagct
     5041 taacccttca ggtagcacga ttgcccgcac cgaaatgcag tataacgaca agggtatgct
     5101 aaagttcata tcaagattcg atgcacaaaa caaccttatt gactaccagg ccattcgcta
     5161 cgagctttac ccctcacgca accggttaaa catgattatt gagtactgat tgccaaaagg
     5221 gttgaaccga atcagcttaa tgatttgccc tgcacttata gcaagcagcc gatcagcata
     5281 atcagctaca aaaacacacc cttcccgcca gcaagcatag ttgttggtaa aacagaacgg
     5341 ataatcgggc taacggctgg cttgctcccc tgatattgga agagtaaaga agaactggct
     5401 accctttccc ggagtacttt cggcccaaat tttaccatta ttcatttcaa caaactcccg
     5461 gcatagtatc aggccaagac ctgtcccctt ttcgttgcgg gttcccgggg ttgagctaac
     5521 ctggctgcca aacagttttt ttaggctatg ggcatcaatt ccaaccccag tgtcctcaac
     5581 gcataccttt acatgtgaat tttcaacgga gctccatatc tgaattttat ctccggcatt
     5641 gcagaacttg atggcgtttg atataaggtt tcggataatc aggtcgagca tatcgcgatc
     5701 ggcataaacc gatgttgatt catccacgtg gttggtaatg acaatattct tctgaagggc
     5761 aacctcgttt agctgctgaa gcctgggagt aaccacctcg ttcaggaaga atctttccgg
     5821 tttcaccttg gttccctgca tttggttttt ggcccaggta agtaggtttt cgagcagggc
     5881 ggttgtgtgg cttacgttgg acgacagctc gctgattatt tccttaaaac tttcctcggt
     5941 gaaatgtccc tccttggcaa tgttaagcat ggtgataagc gaaaacaatg gacttcggag
     6001 atcgtgcgca attatggaaa atagcctatc ctttaaagca ttcaactccc taagctcatc
     6061 cgcctgccgg ctgatttcct ccttctgggc gaaaatttca gcatttttct cgcttaaaag
     6121 cttgttggct ttttgcttct ggttattagc ccaaaacaaa atggcggcaa acataaggag
     6181 aataataatc aacccgatgt
//
LOCUS       DAOOQX010000024         6155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_15550_length_6155_cov_3.427049, whole genome shotgun sequence.
ACCESSION   DAOOQX010000024 DAOOQX010000000
VERSION     DAOOQX010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15550_length_6155_cov_3.427049"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLX53_00850"
     CDS             <1..203
                     /locus_tag="PLX53_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71658.1"
                     /translation="EPVELLTITFVELLRVQTCPPTGGFVCVCTLPLLMASPRFAATS
                     NTPAIRYHFIPTRRVQELASCR"
     gene            complement(475..1572)
                     /gene="ychF"
                     /locus_tag="PLX53_00855"
     CDS             complement(475..1572)
                     /gene="ychF"
                     /locus_tag="PLX53_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOY71659.1"
                     /translation="MGLQCGIIGITNVGKTTIFNCMSSTKAAASSFAFSASKSNISII
                     NVPDSRLYELEKFQPTERIVHTTVEIVDIPGLAKGSNKGEGVGNKFLADIRNCDALIH
                     VLRCFDDPNLPHVDGSVDPVRDIETVNFELQVRDLESIEKKLQRLEKAAKVGDKEAKQ
                     AAAVLGVYKNHLESFQNAATAPVDEQDKKYVEDLFLLSTKPVIYVCNVDDQSAVTGNH
                     YVDKVREFLKNEQTEILVIAGKLEAEIAELENEDDRLAFLSDVGLTEPGVNKLIRAAY
                     SLLNMETFFTVGPKEIRAWTIKKGMTAPQAAGVIHSDLERGFIRAEVMKYNDFITLGS
                     EQACKEKGKLFVEGKNYVVEDGDILHIRFNV"
     gene            complement(1682..2713)
                     /locus_tag="PLX53_00860"
     CDS             complement(1682..2713)
                     /locus_tag="PLX53_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY71660.1"
                     /translation="MFRISSLTSALFFSLAILAACVPAKEFQALQDKNAKCEEEREKL
                     STENEDLSSRSAELQAKNILLEAKVSTLASDSAICAVELSKTREQLATLNERYADLQS
                     IQDGVISGSKDETRKLLAQIQKNQEELQQKEDALKELERKLYQRKLGLDQVQANLDKM
                     SKDLEERNARLVELERMLNSKDSLMNALRKKVADALFGFEGKGLTVSIQNGKVYVSLD
                     EKLMFKSGRYEIDAKGAAAIKQLVPVLEQNTDINIMVEGHTDDVPYRGTGDLMDNWDL
                     SVKRATTIVRLLLNGSKIDPVRVTAAGRSHFLPVDRAKTPEARQKNRRTEIILTPKMD
                     EILKLLEAN"
     gene            complement(3215..4294)
                     /locus_tag="PLX53_00865"
     CDS             complement(3215..4294)
                     /locus_tag="PLX53_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04133 family radical SAM/SPASM protein"
                     /protein_id="HOY71661.1"
                     /translation="MLSSIKKYSFRKFRDSETKRHELNYLFWECTTRCNLNCLHCGSD
                     CSKDSLHRDMPAEDFFQAIDTIENIPQNFTVVFTGGEPLLRKDIELCGKELRKRGFKW
                     SIVSNGHLYDKQRHISLLNAGIGALTISLDGLKESHNWLRNNEKSFDKVINAIELASS
                     SNRINFDIVTCVNQKNINELEQIRELLISKNVKTWRLFTIIPIGRAAHNPDLSLADNQ
                     FVQLMDFISETRKSKGIDVKFSCEGYVGKYELLVRDSYFFCRAGINIGSILIDGSISA
                     CPNIDRSFSQGNIYTDNFYKVWENQFQPFRNREWTRIGQCELCKDFKDCQGNGFHNWH
                     GDKKNVLVCHYEKIEIQRNANILYK"
     gene            complement(4295..4660)
                     /locus_tag="PLX53_00870"
     CDS             complement(4295..4660)
                     /locus_tag="PLX53_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71662.1"
                     /translation="MKRNVTRKIIGGLSLTSAMFVFQACYGTPQDFGLDILIEGQVKS
                     KTSGLPIKGIKVSVADNVQYEVTDEEGKFSFYTEILEGLTLHFQDIDSTQNGLYIDKD
                     TVLTDLKENIYLDIALEEK"
     gene            complement(4856..5533)
                     /locus_tag="PLX53_00875"
     CDS             complement(4856..5533)
                     /locus_tag="PLX53_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71663.1"
                     /translation="MKHQTIFLILSCIILSSCTATKLLTSNVQPTEVTNLKLLEPYSY
                     ISMIKKGNRGQLDDSISSISKQLNINVLKSFNGQIPITGDIILIDSTINKDLEKECEN
                     LILTAERNKNISNLKITPTLDKILEANDTRFGLIIICTGFTRLKGNYGKEIAKGAALG
                     ILTLGMYYQIPVKAYSTIYAMIVDSKEDNVAFYRKSFKQDLEPLDQEVLTNQYKKIFE
                     GYFWTTK"
     gene            complement(5752..>6155)
                     /locus_tag="PLX53_00880"
     CDS             complement(5752..>6155)
                     /locus_tag="PLX53_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71664.1"
                     /translation="ILFNGLIFTFTFITGSPRLLVYDTSWIIQFGFPILFSIIWTSIN
                     RNGFLRISEFNDSTLLPAKIESIMTKSYNRFDSETGNIEYLKKTKLARFFNYFFRENI
                     KIEKTKEEIVIFAKKNILDTIELKIKNVRTN"
BASE COUNT         1675 a         1342 c         1150 g         1988 t
ORIGIN      
        1 gtgagccagt cgaactactc actataacat ttgtcgaact gctcagggtt caaacctgtc
       61 cgcctaccgg cggatttgtg tgcgtctgca cgctgccctt gcttatggct tctcctagat
      121 ttgcagccac cagtaacacc cctgccattc gctaccactt catcccaacc cggcgtgtgc
      181 aagaacttgc atcttgccgg tagtgtagta gtgtttgtcc gacatcccaa ataagggcca
      241 ggccaaaaag accggcgcaa aagcatggtg taattcaacc tttactttag cttaggaggt
      301 aattgttttg aaaagtgaac agaaaattta cctgtcgaat ggaagattta tgcggcaata
      361 aactacattt cgaggaaaca gggacaatct aaaacatcat tgaaagtatg atggtttagc
      421 accagattaa taagaagatt caggaaaaat attgctcagc aatgtagccg gctgctacac
      481 gttaaacctt atatgcagaa tgtcgccatc ctccacaacg tagttcttgc cctcaacaaa
      541 aagtttaccc ttctccttgc aggcctgctc ggaaccgagg gttatgaaat cgttatactt
      601 cataacctca gcacgaatga acccgcgctc caggtcggaa tgaattaccc ctgcagcctg
      661 aggagccgtc attcccttct tgatggtcca tgcacgaatt tcctttggtc caacggtaaa
      721 gaatgtctcc atgttgagca gggagtacgc ggccctgata agcttgttta ccccgggttc
      781 ggtaagtccc acatcggaaa ggaaggccaa ccgatcatcc tcattctcga gttcggcaat
      841 ttcagcttcc agctttccgg ctataaccag tatttcggtt tgctcgtttt taaggaattc
      901 cctcaccttg tcaacatagt ggttacctgt tactgccgat tggtcatcta cgttgcaaac
      961 gtatatgaca ggtttagttg acagcaagaa aaggtcttca acatactttt tgtcctgttc
     1021 gtcaacaggc gccgtagcag cattctggaa tgattccagg tggttcttgt acacgccaag
     1081 cacggcagca gcctgtttgg cctccttatc gccaaccttg gcagcctttt cgaggcgctg
     1141 aagcttcttc tcaattgact ccagatcgcg cacctgcagc tcaaagttca ccgtttcaat
     1201 atccctaact gggtcaaccg acccatcaac gtggggcagg ttggggtcgt cgaagcaacg
     1261 tagaacatgt atgagggcat cgcagttgcg aatatcggcc aggaacttgt tgccaactcc
     1321 ttcgcccttg ttggacccct tggctaaccc gggtatatca acaatctcaa ctgtagtgtg
     1381 aacaatgcgc tcggtgggct gaaacttttc cagctcgtag agccggctat cgggaacatt
     1441 aatgatgctt atgttcgact tgctggcact aaaggcaaaa ctgctggcag ccgccttggt
     1501 gctcgacatg cagttgaaaa tggtggtttt ccccacattg gttatcccaa taattccaca
     1561 ctgtaaaccc ataaacggtg atttaatggg tacaaattta tggcaattgc ccgattatgc
     1621 aaaaacaggc cgaaaaacct cctataacct cagggttgaa cggagtgtag gcaacttctg
     1681 tctagttggc ctcgagtagc tttaatattt catccatttt tggtgtaagg atgatttcag
     1741 ttcggcggtt tttctgcctg gcctcaggag ttttagccct atcaacaggt aaaaaatggc
     1801 tacgtccggc agcggttacg cgaacaggat caattttgct tccattgagc agcaacctaa
     1861 caatggttgt agcgcgttta acgctcaaat cccagttatc catcaggtcg cccgttcccc
     1921 ggtagggaac atcgtcggta tgaccttcaa ccataatgtt gatatccgtg ttctgctcga
     1981 gcaccggcac cagctgtttt atggcagcag cccccttggc atctatctcg taacgccccg
     2041 atttgaacat cagcttctca tccagcgaaa cgtaaacctt gccgttttga atggaaacag
     2101 ttagcccctt gccttcaaac ccaaaaagcg catcggcaac ctttttccta agagcgttca
     2161 ttagggagtc cttgctgtta agcattcgtt cgagctcgac cagacgggcg tttctttcct
     2221 caaggtcctt gctcatcttg tcgagatttg cctgaacctg gtcgagacca agcttgcgct
     2281 ggtaaagctt tcgctcgagc tccttcaggg catcctcctt ctgctgaagc tcctcctggt
     2341 tcttctgaat ttgagccagc agctttcttg tttcatcctt acttccggag attaccccat
     2401 cctgaatact ctgcaggtcg gcgtagcgct cgttaagggt tgccagctgc tcccttgttt
     2461 tgctcagctc aacggcgcat attgcgctat cggaggccag cgtgcttacc tttgcctcca
     2521 gcaaaatatt ctttgcctga agttcggccg atcgggagct taaatcctcg ttttcggtac
     2581 tcagcttttc gcgctcctcc tcacacttgg cattcttatc ctgtagcgct tgaaattcct
     2641 tggcaggcac acatgcagct aggatagcca agctaaaaaa taaagcactt gttaacgatg
     2701 aaattctaaa catctggtaa ggggttaatt tgaaagcaaa tgtacataaa ttttatacat
     2761 gtaaacggac ctataaaatt aaggttttca cctcaattca cttcgtttgg ttcggtgtgt
     2821 gaataaaaaa atacacgcct ttgctgccgc atggttgcct ttgccaagct ctccgccggc
     2881 gggcaaatcg gttttacctt tcggcgacct ttcaaataca ctttaacagc aggtaaagcg
     2941 ccaataggct attccttcta caccttacct ctatttttta gctacctcac gagggggctc
     3001 gcagggtagc gggggaaatc taacttatac catggatttg aagccatagg gtgccgtgtt
     3061 tttattcaca ttatccttag cggcggggtc gtcctgaaat gaccgcacaa cggttgcgtg
     3121 tatgaaacgt tggggattgc gggcttcttc actgtcaaac cgctacgcag tttgagcggg
     3181 ttacaacctt tgatacttga tacaactcct gccattactt atacaaaatg ttggcatttc
     3241 gttgtatttc tattttctca taatgacaga ctaatacgtt tttcttatct ccatgccagt
     3301 tatgaaatcc atttccctgg caatctttaa aatctttgca caattcacat tgaccgattc
     3361 ttgtccattc tctgtttcga aatggttgaa attggttttc ccaaacctta taaaaattgt
     3421 ccgtgtaaat atttccttgc gaaaaagacc tgtcaatatt gggacaggct gaaattgaac
     3481 cgtcaataag aatcgagcca atattaattc ctgctctgca aaagaagtac gaatccctta
     3541 ctaataattc gtattttcca acatagcctt cacaactaaa cttaacgtca attcctttgg
     3601 attttcgagt ttctgaaata aaatccatta attggacaaa ctgattgtca gctaacgaca
     3661 aatcgggatt atgagccgct ctaccgatag gaataattgt aaataatctc catgtcttaa
     3721 catttttcga aatcaataat tccctgattt gttcaagttc attgatattc ttttggttaa
     3781 cacaagtaac aatatcgaag ttgatacgat tggaagatga tgccaattca attgcattta
     3841 ttactttatc gaaactcttt tcattatttc tcaaccaatt atgagattcc ttaagcccgt
     3901 ctaaactaat tgtcaatgca cctattccag catttagtaa tgaaatgtgt ctttgtttat
     3961 cgtataaatg tccatttgaa actatcgacc atttaaaacc tcgttttcgt agttcttttc
     4021 cacaaagttc tatgtccttt cgtaaaagag gttcaccgcc tgtaaataca accgtaaagt
     4081 tttgtggtat attttcaatt gtgtcaattg cctgaaagaa atcctcagca ggcatatctc
     4141 gatgcaaact atcttttgag caatcacttc cacaatgcag gcaatttaaa ttacatcgtg
     4201 tagtgcactc ccaaaacaaa taattcagtt cgtgccgttt tgtctctgaa tctctaaatt
     4261 ttctgaaact gtattttttg attgatgaaa gcatttattt ttcttccaaa gctatatcta
     4321 agtaaatatt ctcttttaaa tctgtcaata cagtatcctt atcaatataa agtccgtttt
     4381 gagtggaatc aatatcttga aaatgtagtg ttaatccttc aagaatttct gtataaaatg
     4441 aaaacttgcc ttcttcatca gtcacctcat attgtacatt gtcggcaact gaaactttta
     4501 ttcctttaat tggcaaacct gatgtctttg attttacttg accttcaatt aatatatcca
     4561 gcccaaaatc ctgaggtgtg ccatagcatg cttgaaatac aaacattgca gatgtcaaac
     4621 ttaatccacc aattattttc ctagttacat ttcgtttcat gttaatcatt ttaatctatg
     4681 atgattttta taattagcag gttactttta caatgaatgt caacagttga cggtatgggt
     4741 cgtggcgact aaaagacatt ttcctgtcgt ggtatacaaa gtttaatttc gtttacaaag
     4801 ctccagtggt tacaaaagat agccatgacc tataccgttt gttagcagct ggcaatcatt
     4861 tagttgtcca gaaatatcct tcaaaaattt tcttatattg atttgtcaag acttcttgat
     4921 ctagtggttc aagatcttgc ttaaatgact ttctataaaa tgcaacgtta tcttcttttg
     4981 aatcgacaat catagcatat atagtcgaat aggcttttac tggaatttga taatacatcc
     5041 ctaaagttaa tatacctagt gcagctcctt ttgcaatttc tttaccatag tttcctttta
     5101 gtctggtaaa gcctgtgcag ataataatca aaccaaatct tgtgtcattt gcctctagta
     5161 ttttatcaag agtaggcgtt attttaagat ttgaaatatt cttgtttctt tcagcagtca
     5221 gaattaaatt ttcgcattct ttctccagat ctttgttaat tgtagagtca atcaggataa
     5281 tatcccctgt aattggaatc tgtccattga aacttttcaa aacattgata tttagttgtt
     5341 ttgaaattga tgatatactg tcatctaatt gtcccctatt accctttttt atcatcgaaa
     5401 tataggaata aggttcaagg agttttagat tagtaacttc agtaggttgg acatttgatg
     5461 ttaaaagttt ggttgctgtg caggatgata aaataatgca actcaaaatc agaaagatgg
     5521 tctggtgttt cattttttat attttgtttg atttattaag tggagtatga tgtaactatc
     5581 tatcttattg tgtagtatgc ttctacttgc tgccaactgt tgtggctaaa aaaagtgcgg
     5641 gttttcgccc tgtttcacta tccgccgtta ataatgttta aaattagtac aaaagtttga
     5701 gtattccttc accctgcatt ttttttgagc cactgttagc accaggtgtt tttaatttgt
     5761 tctaacattt ttgattttca attctatcgt gtcaagaata tttttctttg caaaaatgac
     5821 aatttcttct ttagtcttct caattttaat attttctcta aaaaagtaat tgaaaaatct
     5881 agccaatttt gtctttttca aatactctat attaccagtt tccgagtcaa atctattata
     5941 acttttggtc ataattgatt ctatttttgc aggcaatagc gttgagtcat taaattccga
     6001 aattcttaaa aatccattcc gatttattga tgtccaaata atcgagaaaa gaattggaaa
     6061 tccaaattgt ataatccaag atgtatcgta aaccaataat ctgggactac ctgttataaa
     6121 tgtaaacgtg aaaatcaaac cgttaaatag aataa
//
LOCUS       DAOOQX010000025         5915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_16335_length_5915_cov_4.016041, whole genome shotgun sequence.
ACCESSION   DAOOQX010000025 DAOOQX010000000
VERSION     DAOOQX010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16335_length_5915_cov_4.016041"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1246
                     /gene="atpB"
                     /locus_tag="PLX53_00885"
     CDS             128..1246
                     /gene="atpB"
                     /locus_tag="PLX53_00885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOY71665.1"
                     /translation="MTSFISRYIISAALVVGLVVGVEEVRADDIAGPVETDSTQTEKS
                     KGFNVADFIFEHVGDSYEWHILTVNGHHISIPLPIILYSRTSGLQVFLSNKFHNSTRT
                     YKGFKWEDEGKYAGKIVQLNSAGEVDDANPLPLNFSITKNVLTIFMLGIFLCWLFISI
                     AKQYKKRPNSAPTGIQNMLEPIVLFVRDEIALPSLGEKQYQRFMPFLLTLFFFIWFAN
                     MLGLIPIFPGGANVTGNIAVTMVLAVFTFVVITVNGNKHYWKDIFNAPGVPWFLKLPI
                     PIMPLVEIMGMFTKPIVLMVRLFANILAGHMVGIVFMSLIFIFAGMKFWLGYVAAPIS
                     LIFSIFMTALELLVALIQAYVFTLLSAIYIGMATTEHE"
     gene            1278..1535
                     /gene="atpE"
                     /locus_tag="PLX53_00890"
     CDS             1278..1535
                     /gene="atpE"
                     /locus_tag="PLX53_00890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOY71666.1"
                     /translation="MTTLLILLQAAQGAALGKIGAAIGAGLAVIGAGMGIGSIGKSAL
                     EAIARQPEAAGDIRSNMIVAAALIEGVAFFALIVCILVIFL"
     gene            1548..2042
                     /gene="atpF"
                     /locus_tag="PLX53_00895"
     CDS             1548..2042
                     /gene="atpF"
                     /locus_tag="PLX53_00895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOY71667.1"
                     /translation="MGLVTPDYGLLFWMLLSFSIILYILKKFAWKPIIHALKNREDSI
                     ALSLKQAEQARAEIVKLQEENKALAEKARLEREKEFQETKALRDKLLIEAKEQARLET
                     QKMLDKAREQILSEKEAAKKELHSTIVSMSVEISEKVLRKQLAEKDRQVEYINELLNE
                     MSRN"
     gene            2057..2593
                     /gene="atpH"
                     /locus_tag="PLX53_00900"
     CDS             2057..2593
                     /gene="atpH"
                     /locus_tag="PLX53_00900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOY71668.1"
                     /translation="MSSGRVATRYAKGLLKHGNQKGNSPEIFASVQFMIRILDEVKML
                     REFIDNPAFSTDEKCNKIKELVGDQKHTDIVNFIQLLLKKHREEYLYNSLHIFRNLYL
                     RENNILDVVIESVNELDDNVVSRIEAYISSQFGKKCEIKVEVNPALIAGIVILVDGKV
                     IDYSVKGQLEGFRKKLVG"
     gene            2630..4210
                     /gene="atpA"
                     /locus_tag="PLX53_00905"
     CDS             2630..4210
                     /gene="atpA"
                     /locus_tag="PLX53_00905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOY71669.1"
                     /translation="MADIKPSEISELIKDELADLKYKPKFEEVGFVLQVSDGIARVFG
                     LRNVKSNELVEFESGVKGIALNLEEDNVGVVLLGDTFKVKEGDKVKSLGEIASINVGE
                     GLLGRIINTLGEPIDGKGPIAGKTIQLTLERKAPGVIYRQPVSQPLQTGLKAIDSMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQRNNFLKGDPVYCIYVAIGQKGSTVASLART
                     LEEHGAMEYTTIVVATASDPAAMQFYAPFAGATIGEYFRDTGRHALVVYDDLSKQAVS
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKIINNDSIAANMNDLPEPLRPFVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESNLFNNGIRPAINVGISVSRVG
                     GNAQIRSMRKVAGTLKLDQAQFRELEAFAKFGADLDAATISVLEKGSRNVEILKQGQF
                     KPLPVEEQVAIIFCGTAGLLKNIPINRIADFEKDFLERCELTLKEEMKRIAAGEFTDD
                     IAKALTQLVSSVSKNYNA"
     gene            4349..5221
                     /gene="atpG"
                     /locus_tag="PLX53_00910"
     CDS             4349..5221
                     /gene="atpG"
                     /locus_tag="PLX53_00910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOY71670.1"
                     /translation="MANLKEIRTRISSVTSTRKITGAMKMVSAAKFKKAQNNLLRFRP
                     FYEKINHLAADLIKSSVSNESAYMIEREQVRNIGVVLITSNNSMCGAFNANIIKRALV
                     VLNELNRQYPQAKVIFYPLGKTGEEFLVKNKFNVVSCNNAIIEKPSVERTKDFFNLLN
                     SLYLGNELDRIVLVYNSFKNAATQIQKVETLLPLVTPDEQYGSEDLRIVEPDPATLLD
                     RLLPYYLLNSLHSSILESSAAEHGARMTAMHQATENATTIMNNLILEYNKARQASITN
                     EILEIVSGANALSH"
     gene            5375..>5915
                     /locus_tag="PLX53_00915"
     CDS             5375..>5915
                     /locus_tag="PLX53_00915"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 domain-containing protein"
                     /protein_id="HOY71671.1"
                     /translation="MKKTTMFISIFLVMIMGAVQSSCIGSFKLSKKVLTWNKSLGGKF
                     LNELVYILFWVLPVYEVSMLIDGLILNTIEFWTGSNPVAMNPGEVESKIVEQNGVRYQ
                     VVASQNRFDFTQLDGPQKGQSGALVYSPTTKTWSYEGETLSVELLRLNDDGTTSVLLP
                     EGATVAVDATSGLTASLRSF"
BASE COUNT         1734 a         1144 c         1353 g         1684 t
ORIGIN      
        1 taattcttct tgccggcttg ctgaaaccgg tagtattaat atcactatat ttgaccacat
       61 actttgtgct cttaacccat gaggtggtaa tgcttacaag gttgattaaa aaaaggtaga
      121 tttgaaaatg accagcttca tttcaagata tattattagc gcagctctgg tagttgggtt
      181 ggttgtcggg gttgaagaag ttcgtgcaga tgatattgca ggacctgttg aaacggattc
      241 tacccaaacg gagaaatcga agggttttaa tgtagccgat ttcatttttg agcatgttgg
      301 cgattcttac gagtggcata ttcttactgt caatggacat catataagca ttcctcttcc
      361 tataatactt tatagcagaa ctagcggatt gcaggttttc ttatcgaaca aattccataa
      421 ttccacccga acctataaag gctttaagtg ggaagacgag ggcaagtatg ctggaaaaat
      481 tgttcagctc aacagtgcag gtgaggtaga tgatgcaaac cctctgcccc ttaatttttc
      541 aattacaaaa aatgtgctca ccatcttcat gcttggcatt tttctctgct ggttatttat
      601 ttctatcgcc aaacagtata aaaaaaggcc taattcggct ccaacaggca tacaaaatat
      661 gctggaaccc attgtgctat ttgttcgtga cgaaatcgcc ttaccctcgt taggtgaaaa
      721 gcagtaccag cgattcatgc ccttcctgct aaccctattc tttttcattt ggtttgccaa
      781 catgctgggt cttattccaa tatttcccgg aggagctaac gtaacaggta atattgccgt
      841 taccatggtg ttggccgttt ttacctttgt ggtaattaca gtaaatggaa acaagcatta
      901 ctggaaagat atctttaatg caccaggagt tccgtggttc ttaaagcttc ccattcccat
      961 catgccgctt gttgaaatta tgggtatgtt taccaagcct attgtgctga tggtccgact
     1021 attcgccaac attttggcag gtcatatggt tggaattgtt tttatgagcc taatctttat
     1081 ctttgcagga atgaaatttt ggttagggta tgtagcagca cccatttcgc ttattttttc
     1141 tatatttatg actgctcttg agctgctggt cgctctaatc caagcgtatg tattcacctt
     1201 gctgtcagcc atatatatag gaatggccac aacagaacac gaatagctaa ctaacatttt
     1261 ttataaatct tataactatg acaacattac taattctttt acaagctgcc cagggtgcag
     1321 cattagggaa aattggagcc gctattgggg cagggcttgc tgttataggt gcaggcatgg
     1381 gaattggtag catcggaaaa tctgcgctgg aagcaattgc ccgccaaccc gaagctgccg
     1441 gagatatccg ttcaaacatg attgttgccg ctgcgcttat tgaaggggtg gcattttttg
     1501 ccctcattgt ttgtattctg gttatattcc tttaaaattt gtaagctatg ggcttggtaa
     1561 cccccgatta cggactgctt ttctggatgc ttctatcctt ttccataatt ctctacattc
     1621 ttaaaaagtt tgcatggaaa ccaataattc atgctcttaa gaatagggag gattccattg
     1681 ctctttcgct taagcaggca gagcaagcca gagcagaaat tgtaaaactt caggaagaaa
     1741 acaaggcttt ggcagaaaag gcaagattgg agcgggaaaa ggagtttcag gaaacaaaag
     1801 cccttcgcga caagttgctc atcgaagcca aagaacaggc tcggctcgaa acccagaaaa
     1861 tgttagataa agcccgcgaa caaatactta gtgaaaaaga agcagccaaa aaagagctcc
     1921 actccactat cgtctccatg tcggtggaga tttcggagaa agttcttaga aaacagcttg
     1981 ccgagaaaga ccggcaggtg gagtacatta atgagctgct taacgagatg tcgaggaatt
     2041 gattgctttg tagattatga gtagcggaag ggttgcaacc agatatgcca agggattgct
     2101 aaagcatggc aaccaaaagg gaaattcgcc cgaaatattt gccagcgtcc agttcatgat
     2161 taggattctg gatgaggtta aaatgctcag ggaatttata gacaaccctg cttttagcac
     2221 cgatgagaaa tgtaataaaa ttaaggagct ggttggggac caaaaacata cagatatagt
     2281 taactttatc cagctgctgc ttaaaaagca tcgtgaagaa tatctttaca actcgttaca
     2341 tatatttcga aacctttacc tccgcgaaaa caacatcctg gatgtggtta tagaatcggt
     2401 taatgagttg gacgataacg ttgtcagtag gattgaagct tacataagtt cgcaatttgg
     2461 caaaaagtgc gaaataaaag ttgaggttaa tccagcatta attgcgggca ttgtaatatt
     2521 ggttgatggt aaagtcattg actatagtgt gaaaggacaa cttgaagggt ttaggaaaaa
     2581 gttggttgga tagtgaggtt atactggatg aacacaaaca ttgattgcaa tggcagatat
     2641 taaaccttcg gaaatatcgg agttaatcaa agatgaactt gctgatttga agtataaacc
     2701 caagtttgaa gaggttggat ttgtgcttca agttagcgat ggaattgccc gcgtgtttgg
     2761 tttgaggaat gtaaaatcaa acgagctggt ggaatttgaa tcaggagtta agggtattgc
     2821 gcttaacctc gaagaggata acgtaggagt tgtgctgttg ggagacacct tcaaggtgaa
     2881 agaaggagat aaggtaaaga gtttaggaga aattgcctct attaacgtag gagaaggatt
     2941 gcttggccgc attattaaca cccttggtga gcctattgat ggtaagggcc ctattgctgg
     3001 gaaaacaatc caacttacac tagaaaggaa agcgccaggt gtcatttata gacaacccgt
     3061 ttctcagcca cttcaaacag gactaaaggc tattgattcg atgattccta ttggtagggg
     3121 acagcgagaa cttataattg gtgaccggca aacggggaag actgccattg ccattgacac
     3181 cattatcaac cagcgcaaca acttcctaaa gggcgatccg gtttactgca tttatgtggc
     3241 cattggccag aaggggtcaa ccgttgccag tctggccaga acgctcgaag agcatggtgc
     3301 aatggagtac accacaattg ttgtggcaac agcttctgac cctgcggcca tgcaattcta
     3361 tgcacctttt gccggtgcaa ccattggcga gtacttcaga gataccggaa gacacgccct
     3421 ggtggtgtat gacgacttgt ccaagcaagc tgtttcatac cgtgaagttt ctctacttct
     3481 tcgcagacca cccggtaggg aggcataccc tggtgatgtt ttttatcttc actcacggct
     3541 gcttgagcgc gctgcaaaaa ttatcaacaa cgactccata gctgcaaaca tgaatgactt
     3601 gccggaaccg ttaaggcctt ttgtgaaagg tgggggttcg ttaaccgctc ttccaattat
     3661 cgaaacccag gcaggcgacg tgtcggcata tattcctaca aatgttatat ccataacgga
     3721 cggtcagatt tttctggagt caaatttgtt taataatgga attagacctg cgattaatgt
     3781 gggtatttcg gtatcaaggg ttggtggaaa cgcacaaata aggtcaatga ggaaagttgc
     3841 cgggacactg aaacttgatc aggcgcaatt cagggagctg gaggcatttg ccaagttcgg
     3901 agccgatctt gatgcggcta ccatttccgt gttggaaaaa ggttcgagga atgtcgagat
     3961 ccttaagcag gggcagttta aacccctacc ggttgaggag caggttgcca ttattttttg
     4021 cggcacagcc ggtcttttga aaaatattcc cattaaccgg atagccgatt ttgagaagga
     4081 ttttctggaa cgctgtgagc tcaccctaaa ggaggaaatg aagagaattg ccgcagggga
     4141 gtttactgac gatattgcca aggcgcttac ccagctcgta agctcggttt caaaaaacta
     4201 taacgcctag ccaaatggac tacggtgcca cgtggagctg ttaattttca ttgtggggta
     4261 accatttaca gggttacccc agaatggcct tattacgaac cgctttacaa tgttatactg
     4321 ctggtcatta aatttagtgt aagtaaaaat ggctaacctt aaggagatac gaacaagaat
     4381 ttcatcggta acttcaacaa ggaaaattac cggtgctatg aaaatggtat cggctgctaa
     4441 atttaaaaaa gcccagaata acctgttgcg cttcaggccg ttttatgaaa aaatcaacca
     4501 tctggcggca gatttgataa aatcaagcgt ttcaaacgaa tctgcttata tgatagaaag
     4561 ggagcaagtc cgtaacatcg gagttgtgct tattacctcc aacaacagta tgtgcggagc
     4621 attcaatgcc aacattatta aaagggcttt ggttgtgctc aatgaactta accgtcaata
     4681 cccccaagca aaagtgattt tttaccctct tgggaaaaca ggtgaagaat ttttggtgaa
     4741 aaacaaattt aacgtagttt catgcaataa tgccataatt gaaaagccat cggttgaacg
     4801 aaccaaggat ttttttaatt tgcttaatag cctctaccta ggcaacgagc ttgataggat
     4861 tgtgctggtt tacaatagtt ttaagaatgc agcaactcaa attcaaaagg ttgaaaccct
     4921 tttaccctta gtgacacctg atgagcaata tggatctgaa gatcttcgaa ttgttgagcc
     4981 cgatccggct actctacttg acaggttgct gccgtactac ctgcttaact cattacattc
     5041 atccatactc gaaagttctg ccgctgagca tggtgcaaga atgacagcca tgcaccaggc
     5101 taccgaaaat gccactacca ttatgaataa tctcattctg gagtacaaca aggcaagaca
     5161 ggcttcaatt actaacgaaa ttctcgaaat tgtctccgga gcaaacgctt tgagccacta
     5221 acctctttgc tttattttgg tactgtttgg cttgtataac ttcatactct gtaatggggg
     5281 ttaaattttt tttatattct cgttacataa tgattgcatt ttatgtataa ataaactaaa
     5341 cttgttaaga tgtttaaacc taaatcttta tatcatgaaa aaaacaacaa tgtttattag
     5401 cattttcctg gtgatgatta tgggagcggt acaatcatcc tgcatcggga gttttaaact
     5461 atccaaaaaa gttttaacat ggaacaagag tttagggggt aaatttctaa acgaacttgt
     5521 ttacatactg ttttgggtgc tacctgttta tgaggtgtca atgctaatag acgggttaat
     5581 tttaaacacc atagaatttt ggaccggttc aaaccccgtt gccatgaatc cgggcgaggt
     5641 tgagtcgaaa attgtagagc aaaatggtgt aaggtaccaa gtggttgcaa gccagaaccg
     5701 atttgatttt acccagctgg atggcccaca aaaagggcaa tcgggagcgt tggtttacag
     5761 ccctactacc aaaacatgga gctatgaggg ggaaaccctc tccgtagagc tgttgaggct
     5821 aaatgatgat ggaacaacta gcgttttgct tcctgaaggt gctacggttg ctgttgatgc
     5881 tacatcagga cttacggcat cgcttaggtc gtttg
//
LOCUS       DAOOQX010000026         5831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_16636_length_5831_cov_2.709834, whole genome shotgun sequence.
ACCESSION   DAOOQX010000026 DAOOQX010000000
VERSION     DAOOQX010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16636_length_5831_cov_2.709834"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PLX53_00920"
     CDS             <1..475
                     /locus_tag="PLX53_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOY71672.1"
                     /translation="ATTFAHLDATTVLDRKIAELGIYPAVNPLDSTSRILTPDVVGEE
                     HYNTAQRVKNLLQRYQELQDIIAILGMDELSEEDKLIVHRARRVQRFLSQPFHVAEAF
                     TGTKGVMVNINDTIKGFNMIMDGEVDKYPEAAFNLVGTIEEAIEKGERLLKEAKA"
     gene            534..794
                     /gene="atpC"
                     /locus_tag="PLX53_00925"
     CDS             534..794
                     /gene="atpC"
                     /locus_tag="PLX53_00925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOY71673.1"
                     /translation="MVCDLISPERNIKVENVNYVKVPGTKGSFGILKKHAPIVSSLEP
                     GAVKVILDDKTKLLFAIPSGGFVEVTDDRVTIIAEEVLQQPE"
     gene            1147..1890
                     /locus_tag="PLX53_00930"
     CDS             1147..1890
                     /locus_tag="PLX53_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71674.1"
                     /translation="MKKILLPFPIIILVILTSTLMAKAQQTVVFQASDGLEVTADLYV
                     KGTDLPYIILLHQARYSRGEYREIAPKLVNLGYNCLAVDLRSGGEVNGVVNRTAMLAA
                     GKRIPAEYYNAMSDVEGAIGYVKSKSDKPIVLWGSSYSASLALIAGAKNLRVTAIVAF
                     SPGEYFDQPDFVSSKIGKLAIPVLTLSSKAECQPVRELLSGVNPKLVSYYCPTDSGQH
                     GSKALWQSNPSNGDYWMAITMFFSRLNKN"
     gene            complement(1952..>5831)
                     /locus_tag="PLX53_00935"
     CDS             complement(1952..>5831)
                     /locus_tag="PLX53_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOY71675.1"
                     /translation="TIDIYFSNGSVCVPEGGNLEIGIWVYLKPTGITDNQTITLYIDP
                     LNPLLTAYDNGTQFSSTFPSGIYSSTFTIRVHATHLKFDQTPARVGVNEAFPVVVKAA
                     DINGNTDTDYFGSLGLGLAAGDGHLDVSGGTEGSLQNGIANFIASYSKPGSFALLAME
                     PTLEDVASPFITCGDADGQVYPLFTGSDTLIFTAQNTNTSAALEIFRLKVSDSGTSDG
                     LPLKVKTICLTAFNPNSASSLEKIIGGFMLRLEEKVIIPIEFSQSEGVFYIQLSENQL
                     EIADGDTIEMGVELYLNPAFLKDNEKFQFYIPSSSHGWVAYDDGTAFCPNFLSYIYGL
                     PCSIEVEADRLAFNSYPFAATPNDCFDVEVISTDCFGNPDANQLGQVTISENSASGNI
                     EIPDSTEPLSDGIATWSNIKFLNDGRFTLKASHDTLLYAVTPQIFCGYSQMCLIEEAF
                     EGDIPGWGGVESWRVSTISPVAGGSSLVHAGDPGAEKSELVIPLNANAYGRVVEWNVV
                     VKTGSWDPSTDNYFYMVLGSTDSLFNNATTSGYAVGINPSEVDDFLSLWHFEGGKKTN
                     LITTRFDWSEKDEVEIRVTLSPTGILSLWYKPKDYGRMVNGGEVLIQEAIPMKYAGFV
                     YAYTPTRAGQLWLDNLKLCLTEFPPLVKSVRLSSLNSVKVSFSKNVSITSASIPQNYL
                     VRTAEGEPVAVKSVSIDQSKHDMAVLTTEKLSFGSYTLYISNIEDEQGHFTSDSATFG
                     LGSNGNFGRLVINEIMAKPIPSNGLPEYEYIELYNPSADTVFTKGWRIVLNDLQLNLP
                     LDSVLPRTFTLIGGSTAMSEMAVFGKTMGVTSFPSLLNDGMLIKLIDGEGNLISFASY
                     SSDWYNDDSRSDGGYSLECIDASNIAEGKRNWHACMDELGGTPCKENSVVAINPDITP
                     PKVIYLEVLSEDTIRIAYSEPMDSLTITDIENYSLSSENLGVKKIMLQGNLYDGVKLI
                     LNNPMEPGSFYQLAIGGEIVDFSGNPIEESTFKIGLPQIPAAGDLVVNEVLFNPYSGG
                     VDFVEIFNNSQKCIDLKNLLLANRNTDLSLNGVNPAADTSRLIFPAEYAVITVNPQQV
                     KQFYYCENEKAFVQVSSMASLNSDEGYVVLLNANMDVVDELHYTEKLHNPIITNVKGV
                     SLERINPKLPSADASTWHSAAQSVGFATPTFKNSQYAETSMEDGYFTISPEIISPDGD
                     GKDDFLQLCYSLPAEGYVSNIRIFTSAGVEVFRLANNLLLGREGCLRWDGVNYANRIV
                     PTGIYVVYVEYFNLSGEVKREKRVCVVAER"
BASE COUNT         1647 a         1301 c         1241 g         1642 t
ORIGIN      
        1 tgcgactacc tttgctcacc ttgatgctac taccgtttta gaccgcaaaa ttgccgagct
       61 gggaatttac cccgccgtga accctcttga ctcaacctcg agaatactta cccccgatgt
      121 tgtgggagaa gaacactaca acactgcgca acgggtaaaa aatcttcttc agcgctatca
      181 ggagctacag gatattattg caatcttggg tatggatgag ctgtcggagg aggataaact
      241 tattgtgcac cgagcccgta gggttcaaag gttcctctcc caaccatttc atgttgccga
      301 agcgtttaca ggaacaaagg gcgttatggt aaatattaac gacaccatca agggatttaa
      361 catgattatg gatggtgagg ttgataagta ccctgaagcg gcttttaacc ttgttggtac
      421 cattgaagag gctattgaga aaggagagcg cctgttgaaa gaggctaaag cataacacct
      481 ttaccgacta caataagaga gtattttaga cctgaactaa tatagaaggg caaatggttt
      541 gcgacttgat ttcacccgag aggaacatca aggttgaaaa tgtgaactac gtcaaggtac
      601 ctgggacaaa gggttcgttt ggcattctaa aaaagcatgc tccaattgtt tcatcactgg
      661 aacccggagc tgttaaagtt atccttgacg ataaaactaa acttcttttt gcaatacctt
      721 caggtggttt tgttgaagta actgacgatc gggttacaat cattgccgaa gaggtgctgc
      781 agcaacccga atagcctttc ttaagcacca gttgctggct tgccctactt tgcaatataa
      841 ggagttgttt ctgtattttt gcttttgccc cctctgacct actatttaat aaagttaggc
      901 tttaagctgg tagtttacgc tgaaaggtag aaaaagaggc acaattaatc aacaattgct
      961 atctgataat agtagctttt tcgctccacc tgaaggtgaa taaatttcca tcattttggt
     1021 ttaagtgttc aactctgcag gcatactatc attttagata cttaccaaat ttatccggtt
     1081 actctaacgt taatattttc gccttatacc cacaattatt ccttacagcc aagcaaacct
     1141 aaaggcatga aaaagatttt actaccattc ccgatcatca tactggtaat attaacgtca
     1201 acccttatgg caaaagcaca acaaactgtc gtttttcaag ccagcgatgg actagaggtt
     1261 actgccgacc tttacgtcaa gggtaccgac ttgccctata tcatcttgct ccaccaggca
     1321 aggtacagca ggggtgaata ccgagaaatt gctccaaaac tggttaactt gggctacaac
     1381 tgcctggcgg ttgaccttcg ttcaggtgga gaggtaaacg gggtcgtaaa taggacagcc
     1441 atgctggctg ctggtaaacg aatacctgcc gaatattata atgccatgag cgatgtagaa
     1501 ggggcaattg gctacgttaa gtcaaagtcc gacaagccta tcgtgctttg gggaagttca
     1561 tactcagcct cgctagcatt gatagccggt gcaaagaact taagggttac agctattgtg
     1621 gctttttcac cgggtgagta ttttgaccag cctgattttg ttagtagcaa gatcggcaaa
     1681 ctcgccattc ctgttttaac tctttcgtct aaagccgaat gccaaccagt cagagagctg
     1741 ctctcaggcg taaatccaaa acttgtttct tattattgcc ctaccgattc gggacagcat
     1801 gggtctaagg ctctttggca atcaaacccc tccaacggtg attactggat ggctatcacc
     1861 atgttcttca gccggctaaa caagaattaa ttcttgttta ccggataaaa gtcagaatgg
     1921 aaaagccata ccggcagaga tgcttttgaa gctatcgttc ggccaccacg cacactcttt
     1981 tttcccgttt aacttcacca gataggttga agtactcaac atacacaaca tatattcctg
     2041 ttggaacaat tctgtttgca tagtttacgc catcccatct aaggcatccc tccctcccta
     2101 aaagaaggtt atttgccagg cgaaaaactt ctactccggc agaggtgaat atgcggatgt
     2161 ttgaaacgta tccctcagcc ggcagactat agcaaagctg aagaaaatcg tccttcccat
     2221 ccccatctgg cgagataatt tccggcgaaa tggtaaaata gccatcttcc attgaagttt
     2281 cagcatactg cgaattttta aatgtaggtg tggcaaatcc tacgctttgt gctgccgaat
     2341 gccaagtact tgcatcggca gagggcaact tcgggttaat ccgttcaaga gaaacccctt
     2401 tgacgttggt tataattggg ttatgcaatt tttcggtata gtgcagctcg tcaaccacat
     2461 ccatgttagc attgagcaac accacatagc cctcatcact attcagcgaa gccatagatg
     2521 aaacctgcac aaaggccttt tcattctcac aatagtagaa ctgcttaacc tgttgagggt
     2581 taacagttat aaccgcatat tcagcaggaa aaatcagcct ggaggtatcg gctgcgggat
     2641 ttaccccatt tagcgataaa tctgtattcc tgttagctaa aagtaggttt tttaaatcga
     2701 tgcacttttg agaattattg aaaatttcaa caaagtcaac tcctccgcta tagggattaa
     2761 acagaacctc gtttactaca aggtctcctg cggccggaat ttgaggtaat ccaattttaa
     2821 aagtactttc ttcaataggg tttcccgaaa aatcaacaat ttcacctcca atagctaact
     2881 ggtaaaagct gcctggctcc atggggttgt ttagaataag cttaacacca tcgtaaagat
     2941 ttccttgcag cataatcttt ttcacgccta agttttcaga acttaacgaa tagttttcaa
     3001 tatcggtaat ggtaaggcta tccatcggct cgctataggc aattctaatt gtgtcctccg
     3061 aaagtacttc cagatagata acctttggag gagtaatatc gggattaatg gccactacgg
     3121 agttttcctt gcagggtgtc ccacctagtt catccatgca ggcatgccaa tttcttttgc
     3181 cttcggcaat gttagaggca tcgatgcact cgagcgagta gcccccatca ctcctactat
     3241 catcattata ccagtcactg ctatagcttg caaaagaaat caggttgcct tcgccatcaa
     3301 taagttttat cagcatacca tcgttgagca gcgagggaaa agaggttaca cccatagttt
     3361 tgccaaatac tgccatttca ctcatggctg ttgacccacc aatcaaggta aaagttcggg
     3421 gtaaaacaga atccaaggga aggtttagct ggaggtcatt aaggacaatt cgccacccct
     3481 tggtgaaaac tgtgtcggct gaagggttgt agagctcaat gtactcgtac tctggtaatc
     3541 cattcgaggg aattggctta gccatgattt cgttgataac caagcgtcca aaattcccat
     3601 tcgagcctaa accaaacgtt gcgctatcgg aagtaaaatg gccttgttca tcctcgatgt
     3661 tgcttatata aagcgtatag ctaccaaagg acaatttttc ggttgtaagt actgccatgt
     3721 cgtgtttcga ctgatcaata gacaccgatt ttacagcaac cggttcccct tcggcagttc
     3781 taaccaggta gttttgaggg attgaagcgc tggtaatgga tacattttta ctaaaagaaa
     3841 ccttcaccga gtttaggctg gatagcctta cagatttcac taaaggggga aactccgtta
     3901 ggcaaagttt tagattatcc aaccacagct gtcctgccct tgtgggtgta tatgcataaa
     3961 caaatcccgc atatttcatt ggaatagctt cttggataag cacttctcca ccatttacca
     4021 ttctaccata atccttgggt ttgtaccaaa gcgaaaggat tccggtaggc gaaagggtta
     4081 cacgaatttc aacctcatct ttttcgctcc agtcaaatcg tgttgtaatc aggtttgttt
     4141 tctttccccc ttcgaaatgc cataatgata gaaaatcgtc aacttccgaa gggttgatgc
     4201 caacagcgta accagatgtt gtggcgttgt taaacaaact atcggttgat cccagtacca
     4261 tgtaaaagta gttatcggtt gaagggtccc agctgcctgt ttttaccaca acattccact
     4321 caaccactct gccataagca ttggcattca gaggaataac caactctgat ttttctgcac
     4381 caggatcacc cgcatgtacc agtgaactgc cgccagctac cggcgaaatg gttgaaaccc
     4441 gccagctttc gacaccgccc caaccgggta tatcaccctc aaaagcctct tcaataaggc
     4501 acatttgtga atacccacaa aaaatttggg gcgttacggc ataaagcaat gtatcgtgag
     4561 atgctttaag ggtaaatctt ccatcattta gaaattttat gttgctccaa gtagcaattc
     4621 catcgcttaa aggttctgtt gaatcgggga tttcaatgtt gccacttgcg ctattttcgg
     4681 atatggttac ttgacccagc tggttggcgt ccggattacc aaaacaatcg gttgatataa
     4741 cttccacatc aaaacagtcg tttggggtag cagcaaatgg atagctgtta aaagcaagcc
     4801 tgtcggcttc gacctctatg ctacagggaa gcccataaat atacgacagg aaattgggac
     4861 aaaatgccgt tccatcatcg taagctaccc acccgtgtga ggatgacgga atataaaatt
     4921 ggaatttctc gttatccttt aaaaaggcgg gattaaggta cagctctact cccatttcga
     4981 tagtgtcgcc atcagcaatt tcgagctggt tttcgctgag ctgaatgtaa aaaactcctt
     5041 cagattggct gaattctatt ggaataatca ccttttcttc aagtctcagc atgaaaccac
     5101 caataatttt ttctaaagat gaagcgctat ttgggttaaa tgcagtaagg caaattgttt
     5161 ttacttttag gggtaaccca tctgaagtac cagaatcgga aacttttagg cgaaatattt
     5221 caagggcagc gctggtattg gtattttgtg ctgtaaatat taacgtgtcg ctaccggtaa
     5281 aaagcggata aacctgtccg tcggcgtcgc cacaagttat gaagggtgat gccacgtcct
     5341 caagtgttgg ctccatggca agcagcgcaa agctaccagg tttggagtag ctggctataa
     5401 agttggctat cccgttttga aggctgccct cagtacctcc actaacatcg agatggccgt
     5461 ctcctgctgc tagacctaga cctaagctac caaaataatc ggtatcagta ttgccattta
     5521 tatcagcagc ctttacaacc acaggaaaag cctcgttaac accaactctt gctggagttt
     5581 ggtcaaattt taggtgggta gcatggactc ttatggtaaa ggtgctcgag tatattcctg
     5641 agggaaaagt agaagaaaac tgtgtaccgt tgtcgtatgc agttaaaagc ggatttaatg
     5701 ggtctatata cagcgtgatt gtttggttat cggtaatgcc ggttggtttt agatataccc
     5761 aaatgccgat ttccagattt cccccctccg ggacgcatac agagccatta gaaaaataga
     5821 tgtcaatggt t
//
LOCUS       DAOOQX010000027         5745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_16962_length_5745_cov_3.188049, whole genome shotgun sequence.
ACCESSION   DAOOQX010000027 DAOOQX010000000
VERSION     DAOOQX010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16962_length_5745_cov_3.188049"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2428
                     /gene="infB"
                     /locus_tag="PLX53_00940"
     CDS             <1..2428
                     /gene="infB"
                     /locus_tag="PLX53_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOY71676.1"
                     /translation="RTKVKTPKVVGKIDLDKTSKKKAKEKPEKNSNQKTKEESAIVEN
                     EMQPIVEEKLETVPSVQEVIEKPIEVEREEIYRSTVEKLAGPTIKGKIDITAFETKKK
                     QPVASSDAVKNDKKKRRKRIKKDVGRVQLPEAEQQGTPRSNPPLLVKKADLPVNNKKS
                     NAGGSKKKRPVKQEVDEEAVQRQIKETLAKLTTKGKTKGSKYRREKREILNARQQEEN
                     QRLELEKKILKVTEFVSVNELASMMSVPVTDVIEVCMNLGLIVSINQRLDAETMTIVA
                     DEFGYKVEFVSIELQESVKDQEDDSPEMLKPRPPIVTVMGHVDHGKTSLLDYIRHSNV
                     IAGEAGGITQHIGAYGVELENGKKITFLDTPGHEAFTAMRARGARLTDVAIIVVAADD
                     AVMPQTIEAINHASAAGVPIVFAINKIDKPTADPEKIKGELANLNYLVEDWGGKYQCQ
                     EISAKKGTNVDKLLEKVLLEAEMLDLKANPDRKASGTVIESALDKGKGYVATVLVDSG
                     TLHHGDIILAGIYYGHVKAMFNERGVRIAEAGPSTPVQVVGLNGAPQAGDYFHVMESD
                     KEARELANKREQLVRMQGLKTKKHITLDEIGRRIAIGNFQELNIVVKGDVDGSVEALS
                     DSLIKLSTPQIQVNVIHKAVGQISESDILLAAASNAIVLGFQVRPSMGARKLAEKEEI
                     DIRLYSIIYDAINEIRDAMEGMLSPEIKEEITGTAEVLNVFKISKVGTVAGCIVREGK
                     ISRSNKVRLIRDGIVVYTGDLESLKRFKDDVKEVSAGFECGLNINNFNDIKVGDIVEG
                     FQEVEVKRKL"
     gene            2702..3223
                     /locus_tag="PLX53_00945"
     CDS             2702..3223
                     /locus_tag="PLX53_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOY71677.1"
                     /translation="MTLPTGSYSQEELSWFRAIFDDYYESIRSFAYYKTGSVDLADDV
                     VQEVFLKLWNIRSSVRNDTVKTLLYTIATNLIKNHFKHQRVVYNFQVSKSTQDEAESA
                     DANVRMDDMNRQLQDALASIPDAAREVFLMNRIDGFTYSEIAQRLNLSVKTVEKRMSE
                     AIRLLRNKFGYKL"
     gene            3220..4113
                     /locus_tag="PLX53_00950"
     CDS             3220..4113
                     /locus_tag="PLX53_00950"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOY71678.1"
                     /translation="MTTPKEHTKQVSETGHEELMDAIKALQVPPSTRSKEDAWNMIMQ
                     SIATTVQPKETTRINSLRVWLTTAAAAAIVVVSCFSYLWFSRVSVSSPRGVATDVILP
                     DGSTAKLNADSWLKYPKFYSLVGRKLKIEGEVFFTVTPGRSFVVHDDFKRQVEVVGTK
                     FNMATRGSTFKVACFSGSVMVSNPGSERVQLGKGEVAEAAGKTLKVSKDELHIADSPG
                     WTNGEFKFVGVPLSEVLTELERQFNIKVTAMDFNPNGRTFTGYFTKSSLEQALELVAQ
                     PMSLRYEVSSDSTVVKIYSNK"
     gene            4185..>5745
                     /locus_tag="PLX53_00955"
     CDS             4185..>5745
                     /locus_tag="PLX53_00955"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY71679.1"
                     /translation="MVNRFRIAGAVVVFSLLALMAIPHALMGQALITEKFSNAPLVES
                     LNLISKKYAVKVAFDNEALSGYNINARFNNEPVEKVMSRLLKDTNFEYIVIDNVYVIR
                     RKIAVTTDTPKVNPLKFNGVVRDRNSGESLPYASIKVVNTQLGTAANSDGFFSLAQTQ
                     TDSLAKADSLYIRVSYVGYKPIEMKVARFSHTNSPLVVELDRNDLEIKEVKVTDEYSS
                     IVNHGFRPGELVWSSRQVAQVPTFSGIDAIAPLMLLPGVDGTTESLSGLTVRHSAADK
                     NLVTFDGFTLYNIDHFFGSFSSLNSKVVKDIRILRGGFDAQWGGRASSVIEITGKTGN
                     EHNLVVDAGADELSADVSVEGPIGKKLSYVVAARRSFTDYYRSPLYNSLMGVTRPDFS
                     SVQALANTSDADVVKPTFYYYDANAKVSFKPNIRDIISVSGYTSGDKLTYNQNVQNPY
                     VDENADLSTTGVGFRWARQWGSSFYHNLTIGGSSFNLNYVHQDSIMRYRQASQRDTVL
                     KIILGNINHCHH"
BASE COUNT         1653 a         1199 c         1435 g         1458 t
ORIGIN      
        1 aaggactaag gttaaaacac ccaaggtggt aggaaaaatt gacctggaca aaaccagcaa
       61 aaagaaggct aaagaaaagc ctgaaaaaaa tagtaaccag aaaaccaaag aagaatcagc
      121 tatagttgaa aatgagatgc aacccatcgt tgaggagaag ttggaaacag tcccttcggt
      181 tcaagaggtg attgagaaac ccatagaagt tgagagggaa gaaatatacc gatcaacggt
      241 tgaaaagttg gccggtccta caataaaggg gaaaattgat attaccgcct ttgaaaccaa
      301 gaaaaagcag cctgttgcat cttctgatgc ggttaaaaac gataagaaaa aacgccgaaa
      361 acgaataaag aaagatgttg ggagggttca gcttcccgaa gcggagcagc agggaacccc
      421 aagaagtaac cccccattac tagtgaagaa agccgatttg cctgttaaca ataagaagag
      481 taatgctggt ggtagtaaga agaaacgccc ggtaaaacag gaggttgacg aggaagccgt
      541 tcagcgtcaa ataaaggaaa cccttgccaa acttactacc aaaggtaaaa ccaagggttc
      601 aaagtatcgc agggaaaaac gcgaaattct gaacgcccgt cagcaagagg aaaatcagcg
      661 gcttgagctg gagaagaaaa ttctgaaagt taccgagttc gtgtcggtca acgaactagc
      721 ctccatgatg agtgtaccgg ttaccgatgt aattgaagtg tgcatgaatc taggccttat
      781 tgtttccatc aaccagaggc ttgatgccga aaccatgact atcgttgccg atgaatttgg
      841 atataaggtg gagtttgtca gcattgaact tcaggaatct gtaaaagacc aggaggatga
      901 ttctcccgaa atgcttaaac cccgcccgcc catcgttacc gttatggggc atgttgacca
      961 tgggaaaacc tccctgctcg actatatccg tcattccaat gtaattgccg gcgaggcagg
     1021 tggaataacc caacatattg gtgcctacgg agttgagctg gagaacggca aaaaaatcac
     1081 ctttttggac actcccggtc atgaggcatt tacagccatg cgtgcccgcg gtgccaggct
     1141 cactgatgtg gccattatag ttgtagctgc cgacgatgcc gttatgcccc aaaccattga
     1201 ggctatcaat catgccagcg ctgctggggt tccaatagtt tttgccataa ataaaattga
     1261 taagcccacc gccgaccccg aaaagataaa gggggagctg gccaacctta actacctggt
     1321 tgaggactgg ggcggcaaat accagtgtca ggaaatttcg gccaagaagg gcaccaatgt
     1381 tgacaagctg ttggagaaag ttctgctcga ggccgaaatg ctcgatctaa aagctaaccc
     1441 tgacagaaag gcctcgggaa cggtgattga gtctgcattg gacaagggaa agggatatgt
     1501 agctaccgtg ctggtggatt caggaaccct gcaccatgga gacataatcc ttgccggaat
     1561 atactacggc catgttaaag ccatgttcaa cgaacgagga gttagaatag ccgaggccgg
     1621 cccatcaaca cccgttcagg tggttggact taacggtgct cctcaggcag gcgactactt
     1681 ccacgttatg gagtccgaca aggaagcaag ggagcttgcc aacaagcgcg agcagctggt
     1741 gcgtatgcag gggctaaaga ccaagaagca tataaccctt gatgaaattg gccgccgtat
     1801 tgccattggc aacttccagg agctgaacat cgtggtgaag ggcgacgtgg acggttcggt
     1861 cgaggccctc tccgattccc ttatcaaact ttcaacccct caaattcagg tcaacgtcat
     1921 ccacaaggct gtaggccaaa tttccgaatc ggatattctg cttgctgcag catccaacgc
     1981 cattgtactt ggattccagg ttcgtccttc gatgggggca agaaaacttg ccgaaaagga
     2041 agaaattgac attcgccttt actccattat ctacgatgcc attaatgaaa tcagggatgc
     2101 catggagggc atgctgtcgc ccgaaattaa ggaggaaatt accggcaccg ccgaggtgct
     2161 taatgttttt aagatatcca aggtgggaac cgtggccggc tgtatagttc gggaggggaa
     2221 aattagccgt agcaacaagg taaggttaat tcgtgatgga attgtggttt ataccggtga
     2281 tttagagtct cttaaacgct ttaaggacga tgtcaaagag gtttcggcag gttttgaatg
     2341 cgggttgaac atcaacaact tcaacgatat taaggtgggt gatatagtgg aaggtttcca
     2401 ggaggttgag gtgaaaagaa agttgtagca atactcacgt atgtagggag ataactattt
     2461 tggggtctcc ctttttttat tttgatgttt tgggagtttt atggaagctg taaatttttt
     2521 aagtagtact aaaagaggtg caatagcaca ccgattaacc caatagtgct ctgattaacg
     2581 caggttgaat actttgtgag atagtttgtt ccgttccttc agcgtgccat taaaacaatt
     2641 attattaggc tatgtttaat tgaaaatcat caattacatt tgtttcgtta actaatcgct
     2701 aatgacttta ccaacgggta gctattcaca ggaggagcta agctggtttc gggctatttt
     2761 cgacgactac tatgaaagta tccgtagttt tgcctactat aaaactggga gcgttgacct
     2821 ggctgatgat gtggtgcaag aagttttcct taaactctgg aacatccgtt cctccgttcg
     2881 caacgatacc gtgaaaacat tgctttacac cattgccaca aacctgatta aaaaccactt
     2941 caagcaccag cgggtagtgt ataactttca ggttagtaaa tccactcagg acgaggctga
     3001 atctgcagat gccaacgtaa ggatggacga tatgaaccgt cagcttcaag acgctttggc
     3061 ttccattccc gatgccgcca gggaggtatt cctgatgaac cgcattgacg ggtttactta
     3121 cagtgaaatt gcccagcgcc ttaatttaag cgtgaaaacc gttgagaaaa ggatgtcaga
     3181 agcaatacgt ttgcttagga ataaatttgg ctataagcta tgaccactcc aaaagaacat
     3241 actaagcagg tgagcgaaac agggcatgaa gagttgatgg atgccattaa agctttgcag
     3301 gtacccccgt ccactcggtc gaaagaggac gcatggaata tgattatgca gtcaatagct
     3361 acgactgtgc aacccaagga aaccacaagg attaatagcc tgagggtatg gcttacaacc
     3421 gcagctgcag ctgcgatagt ggttgtttcc tgttttagct acctatggtt ctcaagggtt
     3481 tcggttagct ctcccagagg agtagctacc gatgtgattc tacccgacgg ttccacggct
     3541 aagcttaatg ctgattcatg gttgaagtac ccaaagtttt atagcctggt gggtagaaag
     3601 ctgaaaattg aaggcgaggt tttctttacc gttacaccag gtagaagttt tgtggtgcat
     3661 gatgatttta aaaggcaggt tgaggtggtt ggcacgaagt tcaacatggc aaccaggggc
     3721 tccactttta aggtggcatg tttctcagga agcgtcatgg tgagtaatcc gggttcggaa
     3781 agggtacagc tgggaaaggg cgaggtggcc gaggctgctg gtaaaactct gaaggttagc
     3841 aaggatgagc ttcatatagc tgacagccct ggttggacaa atggagagtt taaatttgtg
     3901 ggtgtcccgc tttcagaagt gcttactgaa cttgaacgcc agttcaacat aaaagttacc
     3961 gcaatggatt ttaatcctaa tggtagaact tttacaggct actttactaa atcgagtctt
     4021 gaacaagccc ttgagctggt agcccaacca atgtcccttc gctacgaggt ttcctctgat
     4081 tcaaccgttg ttaagattta tagcaacaaa tagccgtagg gtttttattc attcggttgt
     4141 attattcaca gggtgaactt tttcgttagg ctaaggttaa atttatggtt aaccggtttc
     4201 gaatagcagg tgctgttgta gtgttttcac ttctggcatt aatggccatt ccccatgcgc
     4261 ttatggggca agcgcttatt accgagaaat tcagtaatgc gcccttggta gaatcactca
     4321 acctgattag caagaagtac gcggtaaaag ttgcctttga caacgaggct ttatcgggct
     4381 ataacataaa cgcaaggttt aataatgagc ctgttgagaa agtgatgtcc cgcttgctaa
     4441 aggacactaa ttttgagtac atcgtaattg acaatgttta cgtcattcgg agaaaaatag
     4501 ccgttaccac cgatactcca aaagtaaatc ccctgaaatt taatggtgtg gttcgcgatc
     4561 gaaacagtgg tgagtcccta ccctacgctt ccataaaggt tgtcaacacc cagctgggaa
     4621 cggccgccaa ttccgatggt tttttctcgc tggcgcaaac acaaaccgat tcgctggcta
     4681 aagctgattc cctgtatatc agggtgagct atgttggcta taagccaata gaaatgaaag
     4741 tagccaggtt tagccacact aattcacccc tggtggtgga actcgatagg aatgaccttg
     4801 aaattaaaga ggtgaaggta accgacgagt actcgtctat agttaaccat ggttttcgtc
     4861 cgggtgaact ggtgtggagt tcgagacagg tagcccaggt gccaaccttt tctggaattg
     4921 atgccattgc acctttaatg cttttacccg gagttgacgg aaccaccgaa tccctgtctg
     4981 gcttaaccgt aaggcattct gctgccgata aaaacctggt aacattcgac ggctttacac
     5041 tctacaacat agaccacttt tttggatcat tctccagcct taacagcaag gtagtaaagg
     5101 atatccgtat tttaagagga ggatttgatg cgcagtgggg tggtagggct tcttcggtaa
     5161 ttgaaataac cggcaaaacg ggaaatgagc ataacctggt ggttgatgca ggcgccgatg
     5221 agttgtctgc tgatgtttcc gtggagggac ctatagggaa aaagttaagc tatgtagtag
     5281 ccgccagacg ttcgtttacc gactactaca ggtcgccgct ctataactcc ctgatggggg
     5341 ttactcgtcc cgatttttct tcagttcagg ctctggccaa cacttctgat gcagatgtgg
     5401 taaagcccac attttactac tatgatgcca acgctaaagt ttcctttaag cctaacattc
     5461 gggacattat ttcggtaagc ggttatacat ctggcgataa gctaacctat aaccagaatg
     5521 tgcaaaatcc atacgttgac gaaaacgccg atttgagcac aacaggtgtt gggtttcgtt
     5581 gggccaggca gtggggaagc tcgttctacc acaaccttac aattggagga tcgtcattta
     5641 acctgaatta cgtgcatcag gatagcatta tgaggtatag gcaggcttcc cagcgcgata
     5701 ccgtattgaa aataattttg ggtaatataa atcactgcca tcatt
//
LOCUS       DAOOQX010000028         5698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_17111_length_5698_cov_3.566897, whole genome shotgun sequence.
ACCESSION   DAOOQX010000028 DAOOQX010000000
VERSION     DAOOQX010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17111_length_5698_cov_3.566897"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLX53_00960"
     CDS             <1..685
                     /locus_tag="PLX53_00960"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009227755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOY71680.1"
                     /translation="VAVMPYFGWARQDRKDKPRVAIGAKMVANMLRAAGVDRIMTMDL
                     HADQIQGFFDVPVDHLYASSVFVPYLKSLQIDNIAIASPDMGGAKRANAYARFMNASL
                     VLCYKNREKANQVDEMTIIGDVRGKNVVILDDMVDTAGTLTKASGLMKDQGALSVRAF
                     ATHPVMSGKSYELIRDSQIDQLVVTDTIPLRSDRDTSKIKVLSVAELFADVIEKVYNY
                     KSISEKFII"
     gene            817..1401
                     /locus_tag="PLX53_00965"
     CDS             817..1401
                     /locus_tag="PLX53_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOY71681.1"
                     /translation="MKTIQLSATLRSETGKKAAKATRKSELIPAIIYGGQKNIMISIG
                     EKDMKKVVYTPDVFVVKLNVDGENFDTIVKEIQFHPVSDAILHVDFYQVSNDKNITIA
                     LPVVLIGQAEGTKQGGKLIQVVRKLKVNGLVKNLPENISIDVTQLELGKSIKVGDLSF
                     KGYTLVDPMSTVIASVKSTRAARETQPTEAAPQQ"
     gene            1413..1976
                     /gene="pth"
                     /locus_tag="PLX53_00970"
     CDS             1413..1976
                     /gene="pth"
                     /locus_tag="PLX53_00970"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOY71682.1"
                     /translation="MKYLVAGFGNIGPEYSNTRHNVGFMVLDALARASNITFGSARYG
                     DVAELRHKGKTFILLKPSTYMNLSGKAVSYWMQKEKIALENLLVVVDDLALPLGVIRI
                     RSRGSDGGHNGLKSTNETLGTQDYARLRFGIGNGYPRGHQVDYVLGEWSDEELKILPE
                     RLAKAGEAILCFGTIGIERTMNLFNNQ"
     gene            1989..2408
                     /locus_tag="PLX53_00975"
     CDS             1989..2408
                     /locus_tag="PLX53_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOY71683.1"
                     /translation="MGAESERVDKWLWMVRVFKTRSLAIDCCRNERVTVNGVPVKASH
                     TVQLNDLVAVRKPPVTLTFRVKGFPGSRIGAKLVDEYAENLTPPEEYQKLDPSFRAFS
                     IYRNPGTGRPTKKERRSLDDFFDSEADDSPWDADDSN"
     gene            complement(2446..3696)
                     /locus_tag="PLX53_00980"
     CDS             complement(2446..3696)
                     /locus_tag="PLX53_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY71684.1"
                     /translation="MKKKTTLYIVIGIVVVAVAAHLLLRPRIAQNSYEKGIVLLDSLQ
                     YGQAYEQLNRAVRFSPKNPDYLFGRGMALAGLNRDSAAIADYTKAIAIDSMNFDYFLN
                     RGVSLRKIGNYTASLRDLNRCISLNDSNSRAFYQRGLLNAAMMEYDQAVKDYNRSIEL
                     DGSNLEAFYSRGESLANISDFKGAIADYDRMIEGKNATPESYKKRGIFKLKIQDFNGA
                     ISDLLVALNDLPNDKELLFSLGEAYSNLKDFDQAIGYLDRAASIDPNDPLIYGTRGGA
                     LLNKGDFKAAIQSFNKAINLKPDYLKAVYGRGIAKAFLKDYRGSIEDFNRAISIDKNF
                     TQAYYNRAISKAIMGNHKDALPDFDIAIKYDPKDAEAFFNRGISRMNVKDTKGGCEDF
                     RKALELGYQPAEQMVKIYCSGSGN"
     gene            complement(3967..4413)
                     /locus_tag="PLX53_00985"
     CDS             complement(3967..4413)
                     /locus_tag="PLX53_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71685.1"
                     /translation="MDGEMRLNEYGELVKTEWQKTGIIRPNIVIDAFVVMPNHLHGIL
                     IITDNYDCGGTLQRASTTKTTEQFGKPTKNSIPTIVRLFKSTTTKQINQLRGGVETRY
                     NVAPRVSTIPMLPLWQRNYYEHIIRDDTELTRIRQYIINNPKRWGG"
     gene            4594..>5698
                     /locus_tag="PLX53_00990"
     CDS             4594..>5698
                     /locus_tag="PLX53_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOY71686.1"
                     /translation="MSSYDTTQYKKFNNITGWAIFAIALVVYLCTIEPTASFWDCGEF
                     IASGYKLEVGHPPGAPFFMLLVRFFTMFAPSAQLVPVFANAMSALASAFTILFLFWSI
                     THLARKIIGSESGSFSASQLILIFSAGAVGALTYTFSDTFWFSAVEGEVYATSSLFTA
                     IVFWAILKWEEAADEPYANRWIVLIAYLMGLSIGVHLLNLLAIPAIVLVYYYKNYTYS
                     HKGFFTALLLSAAILLIVMYGIIQGAVAIASGFELFLVNGLGLPLMSGVVFFILTLIV
                     LLVLGLRYTYRRRKYLLNTVLLGFTMILMGYSSYAVIVIRSSANPPMDQNSPDNMFSL
                     KYYLNREQYGDRPLLYGQSFSAQPVDRTDGRLTY"
BASE COUNT         1385 a         1303 c         1414 g         1596 t
ORIGIN      
        1 tgttgcggtt atgccctatt ttggttgggc caggcaggac cgaaaggata aaccccgcgt
       61 ggccattggc gcaaaaatgg ttgccaacat gcttagggca gctggcgttg accgtattat
      121 gaccatggac cttcatgccg atcagattca aggtttcttc gatgtaccgg ttgaccacct
      181 ttacgcctct tccgtttttg ttccctacct taaaagcttg caaattgata atattgccat
      241 tgcctcaccc gatatgggtg gcgcaaaacg tgccaatgcc tatgcccgat ttatgaatgc
      301 ctcgctggtt ctatgctata agaaccgcga aaaggctaat caggttgacg agatgaccat
      361 tattggcgac gtacggggta aaaacgttgt aatactcgac gacatggttg acaccgccgg
      421 aacgctgacc aaggcttccg gtttaatgaa ggatcagggt gcgctatctg ttagggcatt
      481 tgccacacac cccgttatgt cgggcaagtc atacgagctc attcgcgatt cccagattga
      541 ccagctggtg gttaccgata ccataccgct gcgtagcgac agggacacct ccaagattaa
      601 ggttctctcc gttgccgaac tttttgccga tgttatcgag aaggtgtaca actacaagtc
      661 tatcagcgaa aaatttatta tttagcgttg tgtttttaaa gccttttcat atctttgcag
      721 ccctaaatat ccctcaaaat taacggtgtg atggggggct gaaaaatttt cgagggcagt
      781 cctgagtagc acctattggt taacaaaatt tctacgatga aaacaattca gcttagtgcc
      841 acgctgcgta gcgaaaccgg gaaaaaagca gccaaggcaa cacgtaaatc cgaactcatc
      901 ccggcaatta tttatggtgg ccaaaagaac attatgattt cgattggcga aaaggatatg
      961 aaaaaggttg tttacacccc cgatgttttt gtggttaagc taaacgttga tggtgagaat
     1021 ttcgacacta tcgttaagga aattcaattt cacccagtat ccgatgccat tctgcatgtt
     1081 gacttttacc aggtaagcaa cgataaaaat attaccattg ccctcccggt tgtgctaatc
     1141 gggcaggcag agggcaccaa gcagggtggt aagttgattc aggtagttag aaagctaaag
     1201 gtaaatggtc tagttaagaa tctgcccgaa aatatcagca ttgatgttac tcaactagaa
     1261 cttggcaaaa gtataaaggt tggtgatttg tcgttcaaag gctataccct ggttgatccc
     1321 atgagcactg tgattgccag cgttaagtca acccgtgctg cacgcgaaac tcaaccgact
     1381 gaggctgctc ctcagcagta aattgacatg gattgaagta cctggttgca gggtttggaa
     1441 acataggacc ggaatattcc aacacccgtc ataacgtagg atttatggtg ttggacgccc
     1501 tggccagggc gtccaacatt acttttggaa gtgcccgtta cggtgatgtt gccgagctac
     1561 ggcacaaagg caaaactttt atcctgctaa aaccgtccac ctacatgaat cttagcggta
     1621 aggcggtgag ctactggatg cagaaggaga aaattgccct tgaaaacctc ctggtggtcg
     1681 ttgacgatct tgccctgcct ttaggcgtta ttcgcatacg ctccagaggt agcgatggtg
     1741 ggcataatgg gctgaaaagt accaacgaaa cgcttggaac acaggactac gccaggttga
     1801 ggtttggtat cggcaatggt tatccccgcg gccatcaggt tgactacgtt ttgggtgagt
     1861 ggagcgatga ggagctgaaa atcctccccg aacgattggc taaggccgga gaggcaattt
     1921 tgtgctttgg cacaataggc atagagcgca ctatgaatct atttaacaac cagtaagcta
     1981 accaacaaat gggcgccgag agcgagcggg ttgacaagtg gctgtggatg gtgagggttt
     2041 ttaaaacccg ttccctggcc attgactgtt gccgtaacga aagggtaacg gtgaacgggg
     2101 tgcctgttaa agcctcccac accgttcagc ttaacgactt ggtcgcggtg cgcaaacccc
     2161 ctgttacgtt aacattcagg gtaaagggtt tccccggtag ccgaattggt gcaaagctgg
     2221 tggatgagta tgccgaaaac cttacccctc cagaggagta tcagaagctc gatccgtcct
     2281 tcagggcttt tagcatatac agaaatcccg gaacaggccg gcctaccaag aaggagcgtc
     2341 gttcgctcga cgactttttt gattcggaag ccgatgattc cccttgggat gcggacgatt
     2401 ccaactagat tactccactt tgaagcctaa gttagcgcgt ggcgtttagt tgcccgaacc
     2461 ggagcagtat atcttaacca tttgctctgc aggctggtag cccagctcaa gtgctttccg
     2521 gaaatcttcg cagccgccct tggtatcctt cacgttcatg cgcgaaattc ccctgttgaa
     2581 aaacgcttcg gcatccttag gatcgtattt aatggcgatg tcgaaatcgg gtagggcatc
     2641 cttatggttg cccataattg cttttgatat agcccggtta tagtaagcct gagtgaagtt
     2701 tttatcaata gagatggccc ggttgaagtc ctcgattgac ccgcggtaat ccttcaaaaa
     2761 agctttggca atcccacgcc cgtaaacagc tttcaggtag tcgggcttta ggttgatggc
     2821 cttgttgaac gactgaatag ctgctttaaa atcgcccttg ttgagcaggg ctcctccgcg
     2881 tgtgccatat atcagcggat cgttggggtc tatcgaggca gccctgtcga ggtagccaat
     2941 tgcctggtca aaatccttca ggttggagta ggcctcaccc aggctgaaga gcagctcctt
     3001 gtcgttggga aggtcgttga gggcaaccag caagtcggaa atcgctccat tgaagtcctg
     3061 aattttcagc ttgaagatgc cgcgcttttt gtacgattcg ggggtggcgt tctttccctc
     3121 aatcatcctg tcgtaatcag cgatggcgcc cttaaagtcg gaaatgttgg ccagcgattc
     3181 gcccctgctg tagaatgcct ccaggtttga gccgtctagc tcaatgctgc ggttatagtc
     3241 ctttaccgcc tggtcgtact ccatcatggc tgcgttgagc aggccgcgct ggtagaaagc
     3301 cctggagttg gagtcgttta atgaaataca ccggttaagg tctctcagcg aggcggtgta
     3361 gttgccaatt tttctaaggg aaacacccct gtttaggaag tagtcaaagt tcatggagtc
     3421 aatggcgata gctttggtgt agtcggcaat ggcggcagag tcgcggttaa ggccggcaag
     3481 cgccattccc cttccaaaaa ggtagtccgg atttttcggg ctaaacctga cagccctgtt
     3541 aagctgttcg taggcctgcc catactgcag cgagtcgagc aggacgattc ctttttcgta
     3601 gctattttgt gctattctgg gacggagcag taggtgagct gctaccgcta ccactactat
     3661 tccgattaca atatacaggg tagttttctt cttcatctcc tttgtgttgt ttagttctac
     3721 acgcactgac agcaagccta accactgcct aaccggccca aatttagttt atttgactgg
     3781 ttggaacaac cgcctgttaa attttttgat gtaataagct taacatgtaa ccaaaggagg
     3841 gtagggtgtg gtgtacactg gcgtccggaa agggaatgca atcggctggt tatgtgtgcg
     3901 ggaacgaatg tttatgtatt tatcgcaaaa atgtaccgtg gcaatattta cggcaattta
     3961 ttcaaattat cctccccatc gtttcggatt gttaatgata tattgccgta tgcgggttaa
     4021 ttcggtatca tcccggatga tgtgttcgta ataatttcgt tgccacaatg gtagcattgg
     4081 tattgtggag acgcgtggag ccacgttgta acgtgtctcc acgcctccac gcaattggtt
     4141 gatttgtttt gtggtggtcg atttgaacaa ccgtacaatg gtcggaattg aattttttgt
     4201 gggtttccca aattgttcgg ttgtttttgt tgtggaggca cgttgcaacg tgcctccaca
     4261 atcataatta tccgttataa ttaaaatgcc gtgtaaatga tttggcataa caacgaatgc
     4321 atcaataacg atatttgggc gaataatgcc ggttttctgc cattcggttt ttaccaattc
     4381 cccatattca ttcaaacgca tttcaccatc cacaatgttc ccgaataaac attgccggtt
     4441 gtgcgtaaca atggtaataa aatacgcacc cgtttgggaa taatcgtatc ctggtaatcg
     4501 aatagattca tgaccattag ttggctgtat gtttttattg gttaatcaat gccaaaaatt
     4561 ttacctttgg ggtcaaatca acaggcaaac accatgagca gctacgacac tacccaatac
     4621 aaaaaattca acaatattac aggctgggca atttttgcca tagcgcttgt ggtttaccta
     4681 tgtaccatag agcccacagc gagtttctgg gattgcgggg agttcatagc cagcggttac
     4741 aagctggagg taggacaccc ccccggtgca cccttcttca tgcttttagt gcgctttttt
     4801 accatgtttg caccttcggc acagctggtt cctgtatttg ccaacgccat gtcggcgcta
     4861 gcaagcgctt ttaccattct attcctattt tggagcatta cccatttggc aagaaaaatt
     4921 attggttccg aaagtggaag ttttagtgcc agccagctga ttcttatttt ttcggcagga
     4981 gcagtaggag cgctgaccta taccttttcc gatacctttt ggttttcggc tgttgagggg
     5041 gaggtttacg ccacctcatc gttgtttacc gccattgtat tttgggctat tctaaagtgg
     5101 gaggaggctg ccgatgagcc ctacgccaac cgatggattg ttctgatagc ctatcttatg
     5161 ggtttgtcta ttggcgtgca tctccttaac ctgctcgcaa ttcctgcaat tgtgctggtt
     5221 tactactaca agaactacac ctactcgcat aaaggttttt ttacagcgct gctgctatcg
     5281 gcggctattc tgctcattgt aatgtatggc atcattcagg gggcggtggc catcgcttca
     5341 ggatttgaac ttttccttgt taatgggctg ggactacccc tgatgtcggg agttgttttc
     5401 ttcattctaa ccttgattgt tctgctggtg cttgggctac gctacaccta ccgccgaagg
     5461 aagtacctgc tgaatactgt tttgctaggt ttcaccatga ttcttatggg ttacagctct
     5521 tatgcggtta ttgtaatccg gtcatcggct aaccctccaa tggaccagaa tagtccggat
     5581 aacatgttct cgttaaagta ctacctcaac cgcgaacagt acggcgatag gccgcttcta
     5641 tacggtcaat cgttcagcgc ccaaccggtt gatcgcaccg atgggaggtt aacctatg
//
LOCUS       DAOOQX010000029         5677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_17190_length_5677_cov_3.364283, whole genome shotgun sequence.
ACCESSION   DAOOQX010000029 DAOOQX010000000
VERSION     DAOOQX010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17190_length_5677_cov_3.364283"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2889)
                     /gene="secA"
                     /locus_tag="PLX53_00995"
     CDS             complement(<1..2889)
                     /gene="secA"
                     /locus_tag="PLX53_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOY71687.1"
                     /translation="MAGGSFLSKIFGTKADRDMRELMPMLNTIRETYKKYPSLSNDEL
                     RSESAKLKAKVHDYIAADEAEVERLKQMMESDEIGYDEKEKIYKDVEKLQQTIDDKIE
                     EILNIVLPDAFSIVKETARRFKENETIEVTATQFDKDLAAQKDFVTIQDDKAIYKNRW
                     MAGGNEIVWDMVHYDVQLIGGVVLHQGKIAEMATGEGKTLVATLPVFLNALAGKGVHV
                     VTVNDYLARRDSEWMGPIYEFHGFAVDCIDKHQPNSEARRKAYNADITFGTNNEFGFD
                     YLRDNMAIAPDDLVQRKHHFAIVDEVDSVLIDDARTPLIISGPVPKGDDQMFEEYKPK
                     VEKLVNAQRALITQLLADAKKLIAEGNTDEGGKLLLRAFKGMPKYKPLIKFLSEQGNK
                     ALLLKTENYYMQDNNRNMHLITDDLFFVIDEKLNSVELTDKGNDLITSSSEDSKFFIL
                     PDIGAEIAEIEKMPISHNEKLAQKDELLRDFSVKSERVHTVHQLLKAYTMFEKDVEYV
                     VMDNKVKIVDEQTGRILEGRRYSDGLHQAIEAKERVKVEAATQTFATITLQNYFRMYH
                     KLAGMTGTAETEAGEFWTIYKLDVVVIPTNQPVIRKDMEDLVYKTKREKYNAVIDEIV
                     KLTGENRPVLVGTTSVEVSELLSRMLKLKGIKHNVLNAKQHQREAEIVAQAGLPKTVT
                     IATNMAGRGTDIKLTPETRAAGGLAIIGTERHESRRVDRQLRGRSGRQGDPGTSQFYV
                     SLEDDLMRLFGSDRIARIMDRLGLKEGEVIQHPMISRSIERAQKKVEENNFGIRKRLL
                     EYDDVMNAQREVIYTRRRHALYGERLDIDISNMFWDVCEAMVSSFKGSESYEDFSLET
                     IKNFSLEAPVTEEEYENLKPDEIAERLYHQALESYDRKAASIAKQAYPVIKDVYEKQS
                     AIYENIVVPISDGQKTYQIVTNLKKAYESGGKELIRSFSKSIILYTIDE"
     gene            complement(3305..5035)
                     /locus_tag="PLX53_01000"
     CDS             complement(3305..5035)
                     /locus_tag="PLX53_01000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOY71688.1"
                     /translation="MENFIVSARKYRPSTFKSVVGQTSITTTLKNAIQRKQLAQAYLF
                     CGPRGVGKTTCARIFAKTINCLNLTDENEACNECESCKSFSTNRSFNIHELDAASNNS
                     VEGIRKLIEQVRIPPQIGKYSVYIIDEVHMLSASAFNAFLKTLEEPPAHAIFIMATTE
                     KHKILPTILSRCQIFDFNRIRVEDTVKYLEYIAKTEGIDYEVEALNIIAQKADGAMRD
                     ALSIFDQAVSFSENHITYEKVIENLNVLDYQYYFRLTDNFLSGNMTDSLKIFDELLSK
                     GFDAQQFITGLSQHLRNLLVCKDSDTIQLLEVGAAIAEKYTKMASTCPIDFLVNALQL
                     SNQYDVSFRQSPNQRLHVELLLIRLCGLTQEKKKSESPANIEEGDVVEQKPQKQTKPA
                     QKVKHETYEPAAVNEPSTPIIPKTTSLKGALKKNTVESYTASDIDQPEIPSVAVNLPN
                     DSEYKAITQDILGKAWSSFAESIKEEKPGQYSLLQLYGPELLSDGKAYIRFETQIQVD
                     MFREIKTDLLTFLKQSLKVNPPEILEEIAETDEKNKPRLFTADDKLKFLLQHNPALRK
                     MKQKLNLDLE"
     gene            complement(5162..>5677)
                     /locus_tag="PLX53_01005"
     CDS             complement(5162..>5677)
                     /locus_tag="PLX53_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71689.1"
                     /translation="KYGVFAVTGNHEFYGGFEQKIKYLQQHGVKLLRDSTVLVDSSFY
                     IIGRDDRQSSYMLKRERKSVEELISEVDLRKLTILLDHQPYNLDDAARCGVDLQLSGH
                     THNGQLWPFNYITKAMYEVSHGYYQKGGTHYYVSSGFGTWGPRLKIGNRSEVMVVEIT
                     GNPLNSTPEAS"
BASE COUNT         1315 a         1306 c         1325 g         1731 t
ORIGIN      
        1 ctcatcaatg gtgtagagga ttatggattt tgaaaacgat ctaatgagct ccttacctcc
       61 cgattcgtag gcctttttca ggttggtgac aatctggtag gttttttggc catcagaaat
      121 tggcaccacg atgttctcgt atattgccga ttgtttttcg taaacatcct taatgacggg
      181 ataggcctgc tttgcaatgc tggcagcttt tctatcgtac gattcaagcg cttgatggta
      241 tagccgttcg gcaatttcgt ctggtttaag gttttcgtat tcttcttctg taacaggagc
      301 ttcaagggag aaattcttga tggtttccag ggagaagtct tcgtagcttt cgcttccctt
      361 gaatgaggaa accattgcct cgcaaacatc ccagaacatg ttcgaaatat caatatccag
      421 cctttcgccg taaagtgcat ggcggcgacg ggtatagatc acctcgcgct gggcattcat
      481 aacgtcgtcg tattccagca accgcttacg aataccaaag ttgttctcct caactttttt
      541 ctgagcccgt tcaattgacc tggaaatcat ggggtgctgg ataacctcgc cttcctttag
      601 tcccagccta tccattattc tggcaattct gtcagatcca aaaagccgca tcaggtcatc
      661 ttccagggat acgtagaact gtgaggttcc ggggtcgccc tgacggccag aacgcccacg
      721 tagctgacgg tccacgcgcc tggactcatg ccgctcggta ccaatgattg ctagaccacc
      781 tgcagcacgg gtttcggggg ttagcttgat gtcggtacca cgaccagcca tattggtggc
      841 tatggtaacc gttttcggta gccctgcttg tgccacaatt tctgcttcac gctggtgctg
      901 ctttgcattc agcacgttgt gcttaattcc ttttaacttc agcattcggc tgagcagctc
      961 cgatacctca accgaggttg ttcccacaag taccggcctg ttctcaccgg tcagctttac
     1021 aatttcgtca ataaccgcat tatacttttc gcgcttagtc ttgtatacca aatcctccat
     1081 atccttacgg ataacgggtt ggttggtagg aataaccaca acatcgagct tgtagattgt
     1141 ccagaactcg cccgcttcgg tttcagcggt tccggtcata cctgccagct tatggtacat
     1201 cctgaagtag ttctgcaggg taatggtggc aaaggtttga gtggcagctt caaccttaac
     1261 cctttccttt gcttcaattg cctggtgcaa tccatcggag taccgacgcc cttcgagaat
     1321 acgacctgtt tgctcgtcaa caatttttac cttgttgtcc atcaccacgt actccacatc
     1381 cttttcgaac atggtgtagg ccttgagcag ctggtgaacg gtgtgcacgc gttcggactt
     1441 tacggagaaa tcgcgtagga gctcatcctt ttgtgctaac ttctcgttat ggctgatggg
     1501 cattttttca atttcggcaa tttcagcacc aatatcaggg aggatgaaaa acttggaatc
     1561 ctccgatgag ctggttatta ggtcgttgcc cttatcagta agctcaacgg agttaagctt
     1621 ttcgtcaata acaaagaaca ggtcgtcggt aatcaggtgc atgttccggt tgttatcctg
     1681 catgtagtag ttttcggttt taaggagcag cgccttgttt ccctgttcgc taaggaattt
     1741 gatgaggggt ttatacttgg gcataccctt aaatgccctg agcagtaatt ttccaccctc
     1801 atcggtgttg ccctcggcaa ttaacttttt agcatcggct aaaagctggg taatcagggc
     1861 tcgctgagca tttaccagct tttctacctt gggcttgtac tcctcaaaca tctggtcgtc
     1921 gcctttgggc actggtcccg aaatgatgag gggggttcgt gcgtcgtcaa tcagcaccga
     1981 gtcaacctca tccacaatgg caaagtggtg cttgcgctgc actaaatcat cgggggcaat
     2041 ggccatgttg tcgcgcaggt agtcgaagcc aaactcgttg ttggttccaa aggtaatgtc
     2101 ggcgttgtat gctttccggc gtgcttcaga gttaggttga tgcttatcga tgcagtcaac
     2161 cgcaaagccg tggaactcgt agattggccc catccactcc gagtcgcggc gagccaggta
     2221 atcgttaacg gttacaacgt gaactccttt gccggccaag gcattcagga atactggaag
     2281 ggttgccacc agggttttcc cttcaccggt tgccatttcg gcaattttgc cctgatgcag
     2341 aactactccg ccaatcagct gtacatcgta gtgaaccatg tcccaaacta tctcattacc
     2401 accggccatc catcgattct tgtaaatagc cttatcatcc tgaatggtaa caaagtcctt
     2461 ttgggcggct aagtccttat cgaattgggt ggctgtaacc tcgatggttt cattttcctt
     2521 aaacctgcgc gcggtttcct tcacaatgga gaaggcatcc ggaagcacta tatttaggat
     2581 ctcctcaatt ttatcgtcaa tggtttgctg aagtttctca acatccttgt aaatcttttc
     2641 cttttcgtca tagccaatct cgtctgattc catcatttgc ttcagacgtt caacttcggc
     2701 ttcatcagca gcaatgtaat cgtgcacttt agcctttagt ttggccgatt cgcttctgag
     2761 ctcatcattc gataacgagg ggtatttctt gtaggtttcc cgtattgtat tgagcattgg
     2821 cataagctcc cgcatgtcac ggtcagcctt tgtcccaaat atttttgata aaaaacttcc
     2881 gcctgccatt ttaatttaat tagtttgaag ttaacagtat ttaaaaccag caaatttaac
     2941 cgttttttcc gaataattct gaatgccggg gtttgattgc gttaaaaaat actagattgg
     3001 agagccaagt gtttttacta taattttttt gaaatgggaa agggtttggc caacaatcaa
     3061 aaatagtcaa catagcctag cattctgctt agcgtcgagc tcaaaattcg aacagcttgg
     3121 ttgagaataa aaaatttttt gtacagaagt gtcaaggcgt tccggtttat ttattactgt
     3181 agcccaagca ttaaagttga ttgttgcgca cctggtttga tgatggttgc tagcgttaat
     3241 cgtttgttaa ctgcaatagc ctttgatttt cctctaaata ttgcgcggta atgtgctgtt
     3301 tgggctattc gaggtcaagg tttaattttt gcttcatttt ccggagcgca gggttatgct
     3361 gtagcaaaaa tttaagctta tcgtcggcgg taaaaagccg tggtttattc ttttcgtctg
     3421 tttcagcaat ttcctctaaa atctcgggtg ggtttacttt tagggattgt ttcagaaagg
     3481 tgagcaggtc ggttttaatc tccctgaaca tgtcaacctg aatttgggtt tcaaacctga
     3541 tgtaagcttt gccatcgctg agaagttctg gaccgtaaag ttgaagcagg ctatactgtc
     3601 ccggtttctc ctccttaatc gactcggcaa agctactcca tgccttgccg agtatatcct
     3661 gggttatagc tttatactcg gaatcgttgg gcaggttaac agcaacactc ggaatttccg
     3721 gttggtcaat atcgctagcg gtgtagcttt ctactgtatt ctttttaagc gcacctttaa
     3781 gcgaagtggt tttgggaatt attggggttg aaggttcatt tactgcagct ggctcgtagg
     3841 tctcatgttt aactttctgt gctggttttg tctgtttttg tggtttttgt tcaaccacat
     3901 cgccttcctc tatgttggct gggctttcag attttttttt ttcttgagta agcccgcaga
     3961 gcctaatcag cagcaactca acgtgcagcc tttggttggg actttgtcgg aaagacacat
     4021 catactgatt tgacagctgc agtgcattaa cgagaaaatc gatagggcaa gtggaggcca
     4081 ttttggtgta tttctcagct atggcagcac ccacctccag tagctgaatg gtatcggaat
     4141 ccttgcacac caacaaattc cttaaatgct ggcttaatcc ggtgataaat tgctgggcat
     4201 cgaatccctt cgatagtagc tcgtcgaata tcttcagcga atcagtcatg ttcccactca
     4261 gaaagttatc ggtcaacctg aagtagtact gatagtccag cacgttgaga ttttctatta
     4321 ctttttcgta ggtaatatgg ttttcgctga agctaactgc ctgatcgaag atggagaggg
     4381 catcgcgcat ggcgccatca gctttttggg caatgatgtt caatgcttca acctcataat
     4441 caatgccttc agttttggca atgtactcga ggtacttaac ggtgtcctca acccttatcc
     4501 ggttgaagtc gaaaatttgg caacgcgaca ggatggtggg aagaatttta tgcttctcgg
     4561 tggtggccat gatgaaaatg gcatgggctg gcggttcctc gagtgttttc agaaaggcgt
     4621 tgaaagcgct tgcggacagc atgtgcacct catcaatgat gtaaacgctg tacttcccaa
     4681 tttggggagg tatgcgaacc tgctcaataa gcttacggat accctctacg gagttattgg
     4741 aggcagcgtc gagttcgtgg atgttaaatg acctgttggt gctaaacgac ttgcaacttt
     4801 cgcactcgtt gcatgcctca ttttcatcgg taagattcag gcagttgatg gtttttgcaa
     4861 aaatgcgggc acaggtagtt ttaccaactc cccgaggacc gcagaaaagg taagcttgtg
     4921 ccagctgttt gcgctgaatg gcgtttttta gggttgtggt tattgacgtc tgaccaacga
     4981 cacttttaaa tgttgaaggg cggtacttac gagccgatac aataaagttc tccatgcgtt
     5041 acttccagat tgatggtgcg gtttgccatc acaaagttaa tacatacacc gaaactttca
     5101 agccaaaaat tagctgttgg ccaatttggc tgataaccag caaagtatgc gctaagtcga
     5161 tctagcttgc ttccggagta gaattcagcg ggttaccggt gatttcgaca accattacct
     5221 cggatcgatt tccaatttta agccttggac cccatgtacc gaaacccgaa gaaacatagt
     5281 agtgggtgcc tcccttctgg tagtatccat ggctaacctc atacattgcc ttggtgatgt
     5341 agttgaaagg ccacagctgt ccgttatggg tgtgccccga tagctgcagg tcaacaccac
     5401 accgggctgc atcatccagg ttgtagggct ggtggtctaa aagtatggtt agtttccgaa
     5461 ggtcaacttc cgaaatcagc tcttcaaccg attttctctc ccttttcagc atgtagctgc
     5521 tttgccggtc atctcgacca atgatgtaga aagagctgtc aaccagcacg gttgaatcgc
     5581 gtagcagctt tacgccatgc tgctgaaggt attttatctt ttgctcgaat ccgccataaa
     5641 actcgtggtt cccggtaacg gcgaaaaccc cgtactt
//
LOCUS       DAOOQX010000030         5618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_17425_length_5618_cov_3.439691, whole genome shotgun sequence.
ACCESSION   DAOOQX010000030 DAOOQX010000000
VERSION     DAOOQX010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17425_length_5618_cov_3.439691"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            766..5373
                     /locus_tag="PLX53_01010"
     CDS             766..5373
                     /locus_tag="PLX53_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71690.1"
                     /translation="MSRRPARKPRIAATLGLILLALLSLRINALHQDEGKFSGNPLLF
                     PTEVKTIIPDLSKDDEAYLKAFSIFWLSDSIPEDQKGNIIETANLILPILPESKAIVL
                     DYIKLAATFYRDSYANKNYAPWNGALKQLLLTENLDYNKLLAFFRFTRNTVDSSFLRQ
                     TISYTWKVSPHHSYNFEFSSNQLLARFTNVNLVCITGTDSIYIEGTSGYYNPENDMWV
                     GSKGKVTWVRSGYPKDAIFAMLSSYRFPLSKNSYTVDSVYFTNKDYFDRPARGTLTDK
                     LVKDYKPTTIDYPEFKSYDNWFKIKNLFERVDYEGGYIMRGSRLLGVGSGEQLAKVSI
                     SRNGKPFLKVEGNIMIFERSILTADRASILFRFEEDSIYHNGLYFSYNNNTRTVTILP
                     TERITSQSPFYSSYHKLSIWTDQLSWNIDGDKLIFGASRGSSIGNASFESNNFFNEVY
                     FDQLMGPSDRHPLLEVWNFSVRNKSKTFLATDLAQKLRKPVEEVKIEMMRMAKLGYVL
                     YDFETDEVQVTEKLKHTVMAKFQKVDYDVIRFSSTCPQSQPNAELDIASMNMKINGVN
                     NISVSDSQNVFINPYNQQVIMQKNRNFEFAGEVQAGLFRFYGSKYRFNYDEFKIDLLQ
                     VDSLKLDYQTRYRDGYGRKILQSVANTFHIIGGDIVIDKPSNKSGLQSNPDYPIFHST
                     QKSYVYYDDPSIFNGIYKRDSFYFEIQPFTYVNMDNFEQSDLDFKGILYSRNILAPIE
                     ENLVLRPDNSLGFIKSTPPEGLALYRGKGKVFDKIDLSNRGLTVKGQVGYITSTTRSD
                     DIFLFPDSMVTSAASFTMEKQVAGVEFPNVTGDLHHVKWLPQNGKLFVTKGEKPFKMY
                     DSLGIFSGDLLLQPLGLTGIGSMSIPNAALSASLFEFNADDFTTPVSSLTLYSPANQN
                     VALTTKDVRSKISFKSQTGEFSKTNQSLFATLDELGYEAYLDRFSWNMVTNNLAMQTL
                     MQQQDVKDGKFRVSNMVEGDSIPKGSLFYSVKSSEDSLYFFSAKSTYNTATAELNADS
                     VSYIVVADATIMPKDKKIRVNKPNRMLPLSEATIKANNLTAYHRFFDADVSIISRNSY
                     SGKGHYYYADENDSLQTIYFTQIKVDSARNTVATGTITEPDSFTLSPQFAFVGQVHLS
                     APQKLLYFEGGAKPSYQCTGTTGQWIKFEALIDPRRVLIPITEQPKNLNLHPLISGSV
                     ISEDSIKVYGGLLRNRKNYDDKPLVNASGYLSFNNNNRRFTIAPLHKLSNPDTSGSSV
                     SLQKDFCMVFSDGLVKLPVNLGQVKLNAAGSTTHKLEGNDLTLDLVLQLNFHFNQKSL
                     EAIALELNQSFALEKVDLSRKLYRKALYQLVDSSELHAAISQLDLFGSFSQIPKGYES
                     TITFSDLKMRWNPLTKSFVSKGKLGIGTIGNIQVNRFVNGYIEIFKRRSGDLMTLYLD
                     FGEDRYYVFTYTKSVMQVSSNNPEFVQPIEQQKSSETRLKVKPGEPGYRYLIGTKKAL
                     DQAQQRYNQLMSGDEDKVAPPEDEVVEEGRDKVKEKGEKRN"
     gene            complement(5426..>5618)
                     /locus_tag="PLX53_01015"
     CDS             complement(5426..>5618)
                     /locus_tag="PLX53_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71691.1"
                     /translation="ALWKSHDLPRQHILSRPFISNLFRPFRACKKLFAPYKGLCPLLL
                     IPPLRGLNHQTSSEFGIRQ"
BASE COUNT         1643 a         1354 c         1252 g         1369 t
ORIGIN      
        1 cctacggaac ttgttttcac tagtttacct actttcaacc ataagtttgc tcctacggag
       61 cttcaatgcc gtaggcatgt tcttttggta aaagctgaaa tattgcatac ccccctaatt
      121 cgcttgacaa aaattggctt acttacttag tacggtgcac cagcaaaacc aagactaaaa
      181 gtaggcgaaa acttaagctg gatgacaaaa tccagattct tcgtaaaggg gcaacccacg
      241 gtgttgaggt atcctgcttt aagcaccaga cagcaagcag cctgtttaac ggcctaaaga
      301 gcaatttttc cccggtgata cctcgattta ctagcatctc atcaccttcc gatgttccct
      361 tgggttaatg gtctggagca agagaatgaa gtgctgcgta cgattacttt caggcatggg
      421 ctaaccggta ataaaaaatg aactcaaaaa aaactccatt acagctgaag cggtaaattg
      481 cataagcttt tcggggggaa gctccagggg aaatggcaag gaggggaatt tgtcagggac
      541 aacgatagca attaagaaat aaccaacaag tcatcgtccg tactaccctt gacaaattca
      601 gaacttgcgc tacctttgct taaacaggat gaaagggaaa atgtcccaaa ccgtgttgag
      661 taatgtgtcc aaagagcagg gagttaaacc tttagcagcc tgtaaaatag gtttattacc
      721 attcggaagc tgaatggcag tactaactta atctcatcct ccgtcatgag ccgcagacca
      781 gccagaaagc ctcgaatagc agctacactt gggttgatat tgctggcgct gctgtcgcta
      841 agaataaacg ccctgcatca ggacgaaggg aaattctcgg gcaacccgct cctcttcccc
      901 accgaggtaa aaaccataat tcccgatttg agtaaggatg acgaggccta cctgaaggct
      961 ttcagcattt tctggctttc cgattccatt cccgaagacc agaagggaaa tattatcgaa
     1021 acggccaacc taatactacc cattctaccc gagagcaagg ctatcgttct ggattacatc
     1081 aagttggctg ctacattcta tagggatagc tatgctaaca aaaactatgc accctggaat
     1141 ggcgcgctaa aacagttgct gcttaccgaa aaccttgact acaacaagtt gctggctttc
     1201 ttcaggttta cccgaaacac tgttgacagc tcgttcttaa ggcaaaccat aagctacacg
     1261 tggaaggtaa gtccccatca ttcctacaat tttgaattta gcagtaacca gctgctagcc
     1321 aggtttacca acgtcaacct agtatgcatt acaggtaccg atagcatata cattgaagga
     1381 accagtggtt actacaatcc cgaaaacgac atgtgggttg gaagtaaggg taaagttacc
     1441 tgggtacgct cgggctaccc gaaggatgcc atttttgcga tgctgtcgag ctaccggttt
     1501 cccctttcca aaaattccta cacggtggac tcggtttact tcaccaacaa ggactacttc
     1561 gaccgtccgg cacgcggcac cctcaccgac aagctggtaa aggattataa accgaccaca
     1621 attgattacc ccgagtttaa atcgtacgat aactggttta aaattaaaaa cctgttcgag
     1681 cgggttgact acgaaggagg ttacatcatg agggggtcgc ggctgcttgg cgtgggttct
     1741 ggtgagcagc tggcaaaggt atcaatcagc cgaaacggca agccgttcct gaaagtggaa
     1801 gggaatatta tgatttttga acggtcaatc ctcaccgccg acagggccag cattcttttc
     1861 aggtttgagg aggactcaat ttaccataac ggcctttact tcagctacaa caacaacacc
     1921 cgaacggtaa ccattttacc caccgaacgc atcacctcgc aaagcccttt ctatagctct
     1981 taccacaagc tgagcatttg gaccgaccaa ctttcatgga atattgatgg cgataagctg
     2041 atttttggcg cctcgagggg atcaagcata gggaatgcca gctttgaatc caataatttt
     2101 ttcaatgaag tttacttcga ccagcttatg ggccccagcg accgtcaccc tctactggag
     2161 gtttggaact tttccgtcag gaacaagagc aaaacctttc tggctaccga tctggcccaa
     2221 aagctgcgca agccggtgga ggaggttaaa attgaaatga tgcgaatggc caagctgggg
     2281 tacgtgctct acgattttga aaccgatgag gttcaggtta ccgaaaaact aaagcatacg
     2341 gtgatggcga agtttcaaaa ggttgattac gacgtgattc gcttctcatc aacctgtcct
     2401 caaagccaac ccaacgccga gcttgatatt gccagtatga acatgaagat taacggcgta
     2461 aataacattt cggtgagcga ttcgcaaaac gtattcatca acccatacaa ccagcaagtc
     2521 atcatgcaga aaaaccgtaa ctttgagttc gccggagaag tgcaagccgg cttattcagg
     2581 ttctacggct cgaaataccg cttcaactac gacgagttta agatcgacct cctacaggtt
     2641 gactcgctga aacttgacta ccaaacccgt taccgagatg gctatggccg taaaatcctg
     2701 caaagcgtag ccaacacctt tcacatcatt ggtggcgaca ttgtaattga taagcccagc
     2761 aacaagtccg gactccagag taatcctgac taccccattt ttcatagtac tcaaaaatca
     2821 tacgtttact atgacgaccc ctcaatcttt aacggcatat acaagcgtga ttcgttctac
     2881 ttcgaaatac aacccttcac ctacgtgaat atggataatt ttgagcagtc tgacctggac
     2941 tttaagggta ttctgtattc ccgcaacatc cttgccccca ttgaggaaaa cctggtgctt
     3001 cgacccgata actcgctggg tttcattaag tcaactccac ccgagggttt agcgctctac
     3061 cgtggaaaag ggaaagtttt tgataaaatt gacctgagca acagggggct tactgtaaaa
     3121 ggacaggttg gctacattac ctctaccacc cggtcggacg atattttcct tttccccgat
     3181 tccatggtta ccagtgctgc gtcattcaca atggaaaagc aggttgctgg agttgagttc
     3241 ccaaatgtta caggcgacct tcaccatgta aaatggctac cccaaaatgg taagcttttc
     3301 gttacaaagg gggaaaaacc ctttaaaatg tacgattccc tgggcatatt tagcggcgac
     3361 ttgctactcc aaccattagg ccttactgga ataggaagta tgagcattcc aaacgcagct
     3421 ctttcggcca gcctatttga gttcaatgcc gacgatttta ccacgccggt atcgagccta
     3481 accctgtact caccagctaa ccagaatgtt gcgcttacta caaaggatgt aaggtcgaaa
     3541 atcagcttta aaagtcaaac aggagagttc tccaaaacaa accaaagcct ttttgccacg
     3601 cttgatgagt tgggatacga ggcctacctt gaccgtttta gctggaatat ggttaccaac
     3661 aacctggcta tgcaaaccct gatgcaacag caggatgtga aggacggtaa atttagggtt
     3721 tccaacatgg ttgaaggtga ttccatccca aaggggtctc tcttctactc agtaaaaagc
     3781 agcgaagact cgctttactt tttttcggca aaatcaacgt ataatacagc aacagctgaa
     3841 cttaacgccg atagtgttag ctatatcgtg gtagccgatg ccaccattat gccaaaggat
     3901 aaaaaaatca gggtgaacaa gccgaaccgc atgcttcccc tttccgaagc caccatcaag
     3961 gccaataacc ttacggccta ccaccggttc ttcgatgccg atgtatctat tatcagccga
     4021 aacagctata gcggaaaagg tcactactac tacgctgacg aaaacgattc gcttcaaact
     4081 atttacttta cccagataaa agtggatagc gctaggaaca cagttgctac cggaactata
     4141 accgaaccag acagcttcac gcttagccca cagtttgcct ttgttggaca ggtacacctc
     4201 tccgcgcccc aaaagctact ctatttcgaa ggaggagcca aaccatcata ccaatgcacg
     4261 ggaacaacag gccagtggat aaaatttgaa gcgttgattg atcccaggag agttcttatc
     4321 cccataaccg aacagcccaa aaacctcaac ctccatccgt taatcagcgg gtcggtaatt
     4381 tcggaggatt cgataaaagt atatggcgga cttctgcgta acaggaaaaa ctacgatgac
     4441 aaaccgctgg ttaatgcttc gggatacctg agctttaaca acaataaccg tagatttacc
     4501 attgctccgc tacacaagct aagcaatccc gatacatcgg gtagtagcgt tagcctacaa
     4561 aaggatttct gcatggtttt tagcgatggc ctggttaagc tacctgtcaa cctcggtcag
     4621 gttaagctta atgctgcagg cagcactact cataagctag aaggaaacga tctaaccctt
     4681 gaccttgtgc tacagctaaa cttccacttt aatcagaaat cacttgaggc tattgcccta
     4741 gagctaaacc agtcgtttgc gctcgaaaag gttgatcttt caagaaaact ctaccgaaaa
     4801 gcgctttacc agctagttga ttcatccgag ctgcatgctg ccatcagcca gctggattta
     4861 tttggaagtt ttagccagat tcccaaggga tacgaaagta caatcacctt ttcagactta
     4921 aagatgagat ggaacccctt aaccaaatcg tttgtttcca agggaaagtt gggcatcggc
     4981 acaattggca atattcaggt taaccgtttt gtgaatggtt acatcgaaat ctttaaacgc
     5041 cggtcgggcg acctgatgac cctttacctg gactttggcg aagataggta ctacgttttt
     5101 acctatacaa aaagcgtgat gcaggtttca tcgaacaatc ccgagtttgt tcagcccatt
     5161 gaacagcaaa aatcgagcga gaccaggcta aaggttaaac ccggcgagcc agggtaccgt
     5221 tacctcatag gtacaaaaaa agctctggac caggcccaac agcgttacaa ccagctcatg
     5281 tccggtgatg aggacaaggt ggcacctccc gaggatgaag tagttgagga aggaagggat
     5341 aaggtaaagg agaaaggaga aaagagaaat taaacttatc cgcctaccgg cggagcttgt
     5401 ccggctcctg acggaacttg tccgcctact ggcggattcc aaattcagaa cttgtctggt
     5461 ggtttaagcc ccgaaggggc ggaataagta gcaaggggca cagccccttg tatggagcga
     5521 acaatttttt acaagccctg aaggggcgga ataggtttga aatgaagggc ctggagagaa
     5581 tgtgttggcg aggcaagtcg tggcttttcc aaagagcc
//
LOCUS       DAOOQX010000031         5573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_17594_length_5573_cov_3.611816, whole genome shotgun sequence.
ACCESSION   DAOOQX010000031 DAOOQX010000000
VERSION     DAOOQX010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17594_length_5573_cov_3.611816"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="PLX53_01020"
     CDS             <1..543
                     /locus_tag="PLX53_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOY71692.1"
                     /translation="VPGLFNFENSIAAAAAAITCGISHDELRSGLLSFSGVSRRFDIR
                     FKSSRTVYVDDYAHHPREIEAMIESARQVYPNRKITGIFQPHLYSRTRDFASEFARSL
                     DKLDMAILLDIYPAREQPIEGITSQTIADKMNNPNRLLLDKADLLEWLRKNDVDVLLT
                     MGAGDIDRLAPEIVKIFEAK"
     gene            546..1352
                     /locus_tag="PLX53_01025"
     CDS             546..1352
                     /locus_tag="PLX53_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71693.1"
                     /translation="MNRRTRNILIILGWLLLGSYLVYSLLFTAERAKKVLCRGIRVEV
                     VDSAQYQFVKPGTIRKMILSSTISPIGKPLNSINTHKLEKNISELVAVRDVQVYKSMD
                     GILRITVKQRRPIVRIFNARNQSYYIDDEGLVMPVSSSYAAYVLIASGNIYEPFRVKP
                     NIDITRWNDSLTNGSKPLVCQIFDFARYINHDAFWRAQINQIYVINARNVELIPVVGP
                     HVIAMGSLEGYDRKLKKLKLFYENALPTEGWNKYSRINLMYSNQIVCTKR"
     gene            1357..2673
                     /gene="ftsA"
                     /locus_tag="PLX53_01030"
     CDS             1357..2673
                     /gene="ftsA"
                     /locus_tag="PLX53_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOY71694.1"
                     /translation="MAHSNDYVVAIDLGTTKVVTMVGKMNSNGKLQVVGLSTAESTGI
                     KRGMIQNIEETVKAIQKTVDDVKKQVGLEFHSAYVGIAGQHIRSIKNSNYLNFDTDDR
                     EISQDDIDRLVKDMNLTPVDAGETILHVLPLEFFIDKEPVDNNRPVGMMGRRLDANFH
                     IVIGKTSSAKHIEKCVNRVGLSVADLILEPLASAEAVLTDDEREAGVALIDIGGGTTD
                     LAIYYEGNIRHTAVIPFGGNVITQDIKEGCSILLKQADQLKEIYGIALSDIAPDNKII
                     TIPGISGREPKEISVKNLAGIIQARMEEILDFVLYEINLSGYAEKLSAGVVITGGGAL
                     LKHLSHLIKFKSGYDVRLGFPSEKLSSDSLEKYNHPQLSTALGLALIGLERQSATPVQ
                     DDAKQQNATEPSVDKEFSEVHEKKGSKFNAIKQMISGLFEETDSEM"
     gene            2822..4297
                     /gene="ftsZ"
                     /locus_tag="PLX53_01035"
     CDS             2822..4297
                     /gene="ftsZ"
                     /locus_tag="PLX53_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOY71695.1"
                     /translation="MGEELINFDLPATTESIIKVIGVGGGGGNAVNYMHRMGIKDVDF
                     VLCNTDAQALFNSPVKMKLQLGASLTEGRGAGNKPEIGRQAAIESSQQISDLLLRNTK
                     MVFITAGMGGGTGTGAAPVIAQIAKELGILTVAIVTIPFRNEGKKRIAQAIEGIENIR
                     QHVDSLLVVNNEKILEIYGNLKVTEAFSKADDVLATAAKGIAEIITVHGYINVDFADV
                     ETVMANSGVALMGSGRAAGENRAITAVKAALESPLLNNNDISGARNILLNVTYGQDEI
                     TMEEIGLIIEYVQSAAGDDADLIWGNGCDPQLGDDLKVTVIATGFSSSSIPELYIRQK
                     KVDVLNLDGDIFANRVEKTPLVGGSRLQEANPGIPKVKRNEPEDGRQRVIEFDLSSDE
                     IQVKTIGTPKASTPEIPLPVSKTTGKPASQPQKPDSQQGKGKYGDIDELENVPAYKRK
                     NMPLDPVDHSKPDSISRLTLNEEDGDIFLRPDNSFLHDNVD"
     gene            complement(4397..>5573)
                     /gene="glmM"
                     /locus_tag="PLX53_01040"
     CDS             complement(4397..>5573)
                     /gene="glmM"
                     /locus_tag="PLX53_01040"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOY71696.1"
                     /translation="MGCGLDVVNLGLATTPTVEMAVIHKKTDGGVILTASHNPRHWNA
                     LKLLNEKGEFLSDADGHHVLDLAEGDTLNFAAVDDLGLVEEDSSFIDEHISQILKLRY
                     VDVDAIAGANFTVAIDCVNSVGGLAIPKLLNALGVERVIELYTEPTGNFPHNPEPLPE
                     HLGEISAVVRERKANVGFVVDPDVDRLAIVNEDGSMFGEEYTLVACADYVLSHNPGNT
                     VSNLSSTAALREITRRWGKEYYTSAVGEVNVVERMKEVNAVIGGEGNGGVILPELHYG
                     RDALVGIALFLSHLARSGKKCSSLRAAYPSYFISKNKINLKPGVNPDELLAKLLEQLK
                     QYPVNTTDGLRVDFDDSWVHMRKSNTEPIIRIYAEAQNPEKADELAKKMMEQIVKMF"
BASE COUNT         1505 a         1317 c         1370 g         1381 t
ORIGIN      
        1 gttccgggat tgttcaattt cgaaaattct attgccgctg cggcagcagc aattacctgt
       61 ggtattagcc atgatgagct acgaagcggt ctgctctcat tttctggcgt atcacgacgg
      121 ttcgatattc ggtttaaaag cagtagaacc gtatatgttg acgattacgc ccatcacccc
      181 cgcgaaattg aggccatgat agaatcggcc aggcaggttt acccaaaccg aaaaattacc
      241 ggaatttttc agccccacct ctacagccgt acccgtgatt ttgcttccga atttgcccgt
      301 tcgctcgata agctcgacat ggctattttg ctcgatatat accccgccag ggagcaacct
      361 attgaaggaa taacctcgca gaccattgct gataaaatga acaatccaaa caggctgctt
      421 ctggacaaag ccgacctgct cgagtggctt aggaaaaatg atgtggatgt gcttttgaca
      481 atgggtgctg gcgatattga ccggttggcg cccgaaattg taaaaatatt tgaggctaaa
      541 tagatatgaa ccgtcgaacc cgcaacatac ttattatctt gggatggctt ctgttaggct
      601 catacctcgt ttatagcctt ttatttactg ccgagcgtgc aaaaaaggtt ctttgcaggg
      661 gcatcagggt tgaagttgtt gatagcgccc agtaccagtt tgttaaaccc ggtacaataa
      721 gaaaaatgat cctttcgtcc actatttccc ccatcggtaa accgttgaat tcgattaaca
      781 cccataagct tgaaaaaaac atttccgagc tggttgccgt tcgtgatgtg caggtatata
      841 aatccatgga tggcatcctc agaattacgg tgaaacagcg cagacccatt gttcgcatat
      901 tcaacgcacg aaatcaaagc tactatattg atgacgaggg cttggtaatg cccgtttcga
      961 gtagctatgc agcctatgtt cttattgcga gcggaaatat atatgaacct ttcagggtta
     1021 aaccaaacat tgacattacc aggtggaacg atagccttac caatgggtca aaaccgttgg
     1081 tatgccaaat ctttgacttt gccaggtata tcaaccacga tgccttttgg agggctcaaa
     1141 tcaaccagat ctacgttatc aatgcccgaa acgttgagct gattcccgtt gttggtcctc
     1201 atgttattgc tatggggagc ctggagggct acgaccgtaa gttgaaaaag ttgaagcttt
     1261 tctacgagaa cgcactgcca accgagggtt ggaacaagta tagcagaata aaccttatgt
     1321 actctaatca aatagtttgc acaaaacgtt agtgatatgg cacattcaaa cgactatgta
     1381 gtggcaattg acctgggaac cacaaaggtg gtaaccatgg taggtaagat gaattccaac
     1441 gggaagctgc aggtggtggg ccttagcacc gccgaatcaa ctggcattaa acgagggatg
     1501 attcaaaata tcgaggaaac cgttaaggca atccagaaaa cagttgacga cgttaaaaag
     1561 caagttggac tcgaattcca ttcagcctat gttgggattg ccggtcagca catccgaagt
     1621 atcaagaaca gcaactacct gaactttgat accgacgaca gggaaatctc ccaggacgat
     1681 attgacaggc ttgtaaagga tatgaatctc actcctgttg atgctggtga aaccatactg
     1741 catgttttac ccctggagtt ttttattgac aaggagcccg ttgacaataa ccggcctgtt
     1801 gggatgatgg gccgaaggtt agatgccaac ttccacattg taatcggtaa aacttcatcg
     1861 gccaagcaca ttgagaagtg tgtaaaccgg gtgggcttga gtgtggccga tcttattctt
     1921 gagccgcttg cctctgccga ggccgtttta accgacgacg agcgtgaggc tggcgttgcc
     1981 cttattgata ttggcggtgg cactaccgac ctggcaatat actacgaggg gaacataagg
     2041 catactgctg ttattccttt tggtggaaac gttatcaccc aggacattaa ggagggttgc
     2101 tccattcttc tgaagcaggc cgaccagcta aaggaaatct acggtatagc cctctccgac
     2161 attgcacctg ataacaaaat cattaccatt cctggtatta gcgggcggga gcccaaggaa
     2221 atttcggtta aaaacctggc aggcatcatt caggcccgca tggaggaaat tctggatttt
     2281 gtcctctacg aaattaacct ttcgggctat gcagagaagc ttagcgccgg cgtggtgatt
     2341 accggaggcg gagcgctgct gaaacacctc tcgcacctca ttaagtttaa atcgggttac
     2401 gatgtccggt tggggttccc tagtgagaaa ctttcgtccg actcccttga aaaatacaac
     2461 catccccagc tatccaccgc attgggtttg gcgctaatcg gactcgaaag acagtcggcc
     2521 actcctgtgc aggatgatgc caaacaacaa aatgccaccg aaccatctgt tgacaaggag
     2581 ttctcagagg tgcatgaaaa aaagggcagc aagttcaacg caattaagca gatgatttca
     2641 ggactttttg aggaaaccga tagcgaaatg tgaaccgtgc accaggaacc gaaatgctga
     2701 atcccgagac tgtagaaggg agaatccgcc agtaggcgga caagttccgc cagtaggcgg
     2761 acaagttccg tcaaaagccg gactaaattt tgaattataa atattactaa atagttaagc
     2821 catgggcgaa gagctgatta acttcgattt acctgcaaca accgaatcca taattaaggt
     2881 tattggcgtg ggagggggcg gtggaaatgc tgtaaactac atgcaccgca tggggataaa
     2941 ggatgttgat ttcgtgctat gcaacaccga tgcccaggca cttttcaata gccccgttaa
     3001 gatgaagctt cagctgggcg cctctttaac cgagggacgc ggagccggca acaaacccga
     3061 aattggcagg caggccgcca ttgagagctc ccagcaaatt tccgatttgc ttttacgcaa
     3121 caccaaaatg gtttttatca ctgcagggat gggtggcgga accgggacag gtgctgcacc
     3181 cgtaattgca cagatagcga aggagctggg tattctaaca gtggctattg ttacaattcc
     3241 ttttagaaat gaggggaaaa agcgcatagc tcaagctatt gagggtatag aaaacatacg
     3301 tcagcacgtg gactcactgc tggtggtaaa caacgaaaaa attcttgaaa tttacgggaa
     3361 tcttaaggtt accgaggctt tctcaaaagc cgacgatgtg ctggcaacag ctgccaaggg
     3421 aatagctgag ataattacgg ttcacggcta catcaacgtt gattttgccg acgttgaaac
     3481 cgttatggcc aacagcgggg tagcgcttat gggttcggga agggccgccg gtgaaaacag
     3541 ggctatcaca gcggtaaaag ctgcgctgga atcgccacta cttaacaaca acgacatttc
     3601 aggtgcccgt aatatattgc tgaatgttac ctacggacag gatgaaatca ccatggagga
     3661 gattgggctt attattgagt acgttcaatc ggccgctgga gacgatgccg atttgatatg
     3721 gggtaatggc tgcgacccac agctgggcga tgacctaaag gttaccgtaa ttgctaccgg
     3781 tttcagctcc agcagcattc cggaactcta catacgccag aaaaaagttg atgtgcttaa
     3841 ccttgacggc gatatatttg ccaaccgggt tgaaaaaact cctttggttg gcggcagccg
     3901 gttgcaggag gctaatcctg gtattcctaa ggtaaaaaga aacgagccgg aggatggccg
     3961 tcagcgggtt attgagtttg acctctcatc ggatgagatt caggttaaaa cgataggcac
     4021 gcctaaggcc tccactcctg aaattccgct tccggtgtct aaaacaaccg gaaaacccgc
     4081 cagccagcct caaaaacccg attcacagca gggcaaaggg aaatacggtg atattgatga
     4141 gctggagaat gtgcctgcat acaagcgaaa aaacatgcca ctcgacccgg ttgaccactc
     4201 taaacccgat agcatatcca ggttaaccct taacgaggag gatggcgata tattccttcg
     4261 gcccgacaac tcgttcctgc acgataatgt tgactagaaa ttagcgccaa tgttgaatcg
     4321 ggttggctca gcgctgttgc cggttagctg ccttgccaac caaagccctg ctacctggtt
     4381 actgatggtt gcatggttaa aacatcttta cgatttgctc catcattttt ttagccagct
     4441 cgtcggcttt ttccgggttt tgggcttcgg cgtaaatcct gatgatgggt tcggtgttcg
     4501 acttgcgcat gtgaacccat gagtcgtcga agtcaaccct cagcccatcg gttgtgttta
     4561 cgggatattg ttttagctgt tccagcagct ttgccagcaa ctcgtcgggg ttgacgcctg
     4621 gcttcaggtt aattttattt tttgagatga agtagctggg ataggccgct cttagggagg
     4681 agcatttttt gcccgatcgg gcaagatgcg acaggaaaag ggcaataccc accagggcat
     4741 cccttccata atgcagctcc ggtagaataa ctccaccatt accctccccg ccaattacgg
     4801 cattcacctc tttcattcgc tccaccacat ttacctcgcc cactgccgaa gtgtagtatt
     4861 cctttcccca tctacgggtt atttcacgaa gcgctgcggt gctcgacagg tttgaaacgg
     4921 tattacccgg gttatggctg agcacgtaat cggcacaggc caccagggtg tactcctcac
     4981 caaacatgct tccgtcctcg ttaacaatgg ctagccggtc aacatcgggg tcaaccacaa
     5041 agcctacatt agccttgcgc tcacgtacaa cagctgaaat ttcgcccaga tgctctggaa
     5101 gaggttcggg gttatgtgga aaattaccgg ttggctcggt gtagagttca atcacccttt
     5161 caacacccaa tgcgttcaac agctttggaa tggctagtcc tccaaccgaa tttacacagt
     5221 ctatagccac tgtaaaatta gcaccggcaa tggcgtcaac atctacataa cgcagcttga
     5281 gtatttggct gatatgctca tcaatgaaag agctatcctc ctcaaccagc cccagatcgt
     5341 caactgctgc aaagttaagg gtatcgcctt cggcaaggtc aagcacatga tgcccgtcgg
     5401 catcggagag aaattcacct ttttcgttca gcagctttag ggcgttccaa tggcgggggt
     5461 tatggctggc tgttagaatt actccgccat cggttttctt atggattaca gccatttcaa
     5521 ccgtaggagt agtggccagg ccaaggttaa caacatccaa accgcacccc att
//
LOCUS       DAOOQX010000032         5570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_17609_length_5570_cov_3.012511, whole genome shotgun sequence.
ACCESSION   DAOOQX010000032 DAOOQX010000000
VERSION     DAOOQX010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17609_length_5570_cov_3.012511"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLX53_01045"
     CDS             complement(<1..698)
                     /locus_tag="PLX53_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71697.1"
                     /translation="MKSFRLVTFSLVGLTVVLASCFNSGKKPMPLARVYNSYLYPSDI
                     ANLIVGNVSKEDSALIVDAYIDKWIQTQLMLKIAENNLSDSQKNVTRQLEDYRTSLLI
                     FKYEQAYLNQKLDTVIKMADLKAYYDDNKDNFVLNETIVKALYIKLRREAPQIKKIRE
                     LYLSNSDEDIKQLDNIAYQAATKYDYFNDKWIPLSFILRELPFPIANPQREVFEKRYV
                     EMEDGDFVYLVRFM"
     gene            complement(707..2692)
                     /locus_tag="PLX53_01050"
     CDS             complement(707..2692)
                     /locus_tag="PLX53_01050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOY71698.1"
                     /translation="MNKIIRNTLAGFMVITCGIAAVAQPTEQKILLTINNEPVTAGEF
                     QGLYLKSTSIENAKPTREVLEDYLNLFINYKLKVKAAKDNGIDTTAAFKNEYQGYVEQ
                     LAQSYLIDNQVLNNLIEEAYNRMQYEVSASHILISIPENASPSDTLKCYQKAMEARSR
                     ILKGETFGSVAVAMSNDPSVSRNSGFLGYFTAFQMVYPFESAAYSTPVDSISMPVRTR
                     FGYHIIKVHDKRPARGQVKVAHIMIRVPPNPSPQAAEDARLKILGIYQRLTAGADFAQ
                     LAKDESQDPSTSKSGGELPWIRPGQVIPEFEAVAFSLQHDGEISQPFQSSAGWHIVKR
                     LAKKELGNREELIPEIRAMLMRDSRGMLPRTAFIENLKKEYSFKQDTSKLKLLIPLLD
                     SSLYKGTWKSPNLKSNSTLISFNGNELKLSDFCNFIEQNQGLIGKHSIPAFLMNAYTE
                     WVNLTLTSYEKSKLPDKYPEFAQLTREYYEGMLLFEISNREVWQKSSDSLGIANFYNE
                     HQDSYKWGKRIHFAVYTLTDPKIKKAFVSGIEKRSKKGLSPLDYANRFNSPAKQVATV
                     VEKSGDPTLPEVADYNSWPKKFSVRDTQNGEVVVVEVLDTTIGDVKPLTDCKGEVISD
                     YQNSLEREWLEKLKKHYTVIVNRSVFDEISAWQNNNK"
     gene            complement(2879..4360)
                     /gene="guaB"
                     /locus_tag="PLX53_01055"
     CDS             complement(2879..4360)
                     /gene="guaB"
                     /locus_tag="PLX53_01055"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOY71699.1"
                     /translation="MSFQSEKVIAEGLTFDDVLLVPAYSEVLPRNVNLSTKFSRNIDL
                     MAPIVSAAMDTVTESTLAIAIAREGGIGVIHKNMSIEAQARQVREVKRAENGMIFEPI
                     TISPDATIGDALTLMRENHIGGIPVIDSLGFLIGIVTNRDLRFHEDVSTPIRQVMTSD
                     NLITTSKSIDLENATEILKKYKIEKLPVVDENNKLVGLLTYKDITKVKDNPNACKDKM
                     GRLRVAAGVGVTRDTLERVRALCEANVDAIVIDTAHGHSKGVVDMLKLVKREFPNIDV
                     VVGNIATAEAAKMLAGAGADAVKVGIGPGSICTTRIIAGVGVPQLNAIWDVSHALEGS
                     GIPVIADGGIRYSGDIVKALAAGADSIMTGSLFAGVEESPGETIIYNGRKFKSYRGMG
                     SIDAMQQGSKDRYFQDVEDDIKKLVPEGIVARVPYKGTLSEVFYQLVGGLRAGMGYCG
                     TTSIEMLKKARFVKITHAGIIESHPHDVAITREAPNYSRDTDY"
     gene            4589..>5570
                     /locus_tag="PLX53_01060"
     CDS             4589..>5570
                     /locus_tag="PLX53_01060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOY71700.1"
                     /translation="MDRFEEILKQYWGYNIFRSIQRDIIESVYNGNDTLALMPTGGGK
                     SITFQVPALALEGTCLVITPLIALMKDQVENLKKRDIRAVAVHSGLTRHEVDVALDNC
                     AYGDVKFLYLSPERTTTELFRERLRKIKVSMVAVDESHCISQWGYDFRPSYLRIAELR
                     EIISEPVPFLALTATATPEVVEDIQQKLKFRNGKVFSMSFARENLVYLVRNVEDKPKH
                     LLKITHGIPGSGIVYVRSRSKTREIAQMLQKEGISADFYHAGLTMEVRNAKQNDWQVG
                     KTRVIVATNAFGMGIDKPDVRFVVHVDLPDSPEAYFQEAGRAGRDQKLAYA"
BASE COUNT         1383 a         1256 c         1279 g         1652 t
ORIGIN      
        1 tccataaacc ttaccaggta aacgaagtcg ccatcctcca tttcaacata tcttttctca
       61 aaaacctccc tttgcggatt tgctattgga aaaggaagtt ctcgaaggat gaatgagagg
      121 ggaatccact tatcattaaa gtagtcgtac tttgttgcag cctgataggc tatgttatcg
      181 agctgcttaa tatcttcgtc actgtttgac aggtagagct cccttatttt ctttatttgt
      241 ggagcctccc tgcgcagctt gatgtacaaa gccttgacaa ttgtctcgtt tagcacaaaa
      301 ttatccttgt tgtcatcgta gtaagccttc agatcggcca ttttaattac ggtatccagc
      361 ttctggttga ggtaggcctg ttcgtacttg aaaattagaa gtgaagtacg gtagtcctcc
      421 agctggcggg taacattctt ttgagaatcg gatagattgt tttcggcaat tttcagcata
      481 agctgggttt gaatccattt atcaatgtag gcatctacaa tcagggcgct gtcttccttc
      541 gatacgttgc ctacaattaa gttggcaatg tccgaagggt aaaggtagct gttgtaaacc
      601 cttgccaggg gcatgggctt tttacctgag ttgaagcacg atgccaaaac aactgtcaac
      661 ccaaccagcg aaaaagttac taaacgaaaa cttttcatgt tttatctcat ttattgttgt
      721 tttgccatgc agaaatctcg tcgaacacac tcctattaac tataacggtg tagtgttttt
      781 tcaacttttc gagccattcc ctttcaagcg agttttggta atcggatatc acctctcctt
      841 tgcagtcggt cagaggtttt acgtcaccaa ttgttgtgtc caacacctca acaacaacca
      901 cctcgccgtt ttgtgtatcc ctaacactga actttttagg ccaggaattg tagtccgcga
      961 cctcaggcaa ggtgggatca cctgattttt cgactacggt ggccacttgt ttagccggcg
     1021 agttgaaccg attcgcataa tccagaggcg aaagaccctt tttggaacgt ttttctatgc
     1081 cgctgacaaa cgccttttta attttcggat cggtaagagt ataaacagcg aagtgaatgc
     1141 gtttgcccca cttgtagcta tcctggtgtt cgttgtaaaa gtttgctatt cccaggctat
     1201 cagaactttt ctgccacacc tcccggttgg aaatttcgaa cagcagcata ccctcgtagt
     1261 actcgcgggt aagctgggca aattcggggt acttatcagg tagcttagac ttctcgtagc
     1321 tggtaagagt taggtttacc cattcggtgt aggcattcat gagaaaggca ggaatagaat
     1381 gcttgcctat taacccttgg ttttgttcaa tgaaattgca aaagtcagaa agttttagct
     1441 cgtttccatt gaagctaata agggtagagt ttgatttgag gttgggcgat ttccatgttc
     1501 ccttgtagag tgatgaatcc agtaacggaa tcagtagctt caactttgaa gtatcctgct
     1561 taaaggaata ctcctttttg agattctcta taaatgctgt tcggggaagc attcctcgtg
     1621 aatcgcgcat tagcatagct cttatttcag gaataagttc ttccctgttc cccagctcct
     1681 tttttgcaag tcttttcaca atgtgccaac cagctgaact ttggaagggt tgcgatattt
     1741 ccccatcatg ctgcagggag aaggcaacag cttcaaactc tggaattacc tgaccgggac
     1801 gtatccatgg caactctcct cccgattttg aggtgctggg gtcttgcgat tcgtctttgg
     1861 ctagctgggc aaaatcggca ccggcagtaa ggcgttggta aatacccaga atttttaacc
     1921 tggcatcctc ggctgcttga ggggaaggat tgggtggaac tcgtatcatg atatgggcaa
     1981 cctttacctg tccgcgagca ggtcgcttgt cgtgcacctt gatgatgtgg tatccaaagc
     2041 gggttctcac gggcatcgag atggaatcaa caggggtgga gtaggcagct gattcgaaag
     2101 ggtaaaccat ttgaaatgcg gtgaagtagc ctagaaagcc actgttcctc gaaaccgagg
     2161 gatcgttgct cattgcaact gctactgagc caaatgtttc tccctttaga attcttgaac
     2221 gagcctccat ggccttctgg tagcacttta acgtgtcgga gggtgaagca ttttcgggaa
     2281 ttgatatgag tatgtggctg gcgctaacct catactgcat ccggttgtat gcttcctcaa
     2341 taaggttgtt taatacctga ttgtcaatta ggtatgactg tgccagctgc tctacatatc
     2401 cctggtactc atttttaaaa gctgcagttg tatctatccc gttgtctttg gctgctttaa
     2461 cttttaactt atagttaatg aagaggttaa ggtaatcttc tagaacttcg cgggttggtt
     2521 ttgcgttttc tatggatgta cttttaaggt aaagtccctg aaattctcct gcagtaacag
     2581 gttcgttgtt tatagtaagt aaaatttttt gttcggttgg ttgggcaaca gcagctatac
     2641 cgcaggttat taccatgaaa cccgccagag tattcctaat aattttattc atacgaatac
     2701 ttgtttaaag tttactgcca acaaattgca gcagtttcgt tttcatctta gctattttac
     2761 accttcgcca tattccttaa attgatggta cagccaacaa cctttgcgta gttatttttg
     2821 atttttaatg cttctgctgg ggcatggctg cgggcaaaat aataaaacgt gcaagctact
     2881 agtagtccgt atcgcggctg tagttaggag cctcacgagt aatggcaaca tcgtggggat
     2941 ggctctcaat tataccggcg tgggtaatct tgacaaaccg ggcttttttt agcatctcaa
     3001 tgctggttgt accacagtag cccattccag cccttaatcc acccaccagc tggtagaata
     3061 cttcagagag cgttcccttg tagggaactc gggcaactat accctcggga accagttttt
     3121 ttatgtcgtc ctcaacatcc tgaaagtacc tgtcctttga accctgctgc atagcgtcaa
     3181 ttgagcccat gccacggtac gacttgaatt tacgcccgtt gtaaataatg gtttcgcctg
     3241 gagattcctc aacgccggca aagagcgagc cggtcattat ggaatcggcc ccagctgcta
     3301 atgccttaac aatatcgcct gagtagcgga taccaccatc ggcaatcacc ggaataccgc
     3361 ttccctcaag ggcgtgcgag acgtcccaaa tcgcattaag ctgtggaacg cccacgccgg
     3421 caataatacg ggttgtgcaa atggaacctg gtccaatacc taccttaacc gcatcggctc
     3481 ctgcaccggc tagcatcttg gctgcctcgg ccgttgcaat atttccaaca acaacgtcaa
     3541 tgtttggaaa ttctcgcttt accagcttga gcatatccac cacacctttc gagtgtccgt
     3601 gggctgtatc aatcactatg gcgtccacat ttgcttcgca gagtgccctt acgcgttcca
     3661 gcgtatctct ggttacacca accccagcag ccacccttaa ccttcccatt ttatccttgc
     3721 aggcatttgg gttgtcctta accttggtta tgtccttgta ggtaagcagt cctacaagct
     3781 tgttgttctc atcaacaacg ggcaacttct ctattttgta ctttttgagt atctcagttg
     3841 cattttcgag gtctattgat tttgaggtgg ttatgaggtt gtcggaggtc atcacctgcc
     3901 ggattggagt gcttacatct tcgtgaaagc gaagatctct gttggttaca atacctatta
     3961 aaaatcccag gctgtcaata accgggattc ctccaatgtg gttttcgcgc atgagcgtta
     4021 gtgcgtctcc aatagtagcg tctgggctaa tagttatggg ttcgaaaatc atgccatttt
     4081 ctgcgcgctt aacctcacgc acctgtctgg cctgtgcttc gatgctcata tttttatgga
     4141 taacacctat tcccccttcc ctggcaatag caatggcaag ggttgactcc gtaactgtat
     4201 ccattgcagc cgaaacaata ggagccatga ggtcaatatt cctggaaaac tttgttgaaa
     4261 ggttcacatt ccgtggaagc acctcggagt aggccggtac taaaagcaca tcatcgaatg
     4321 taagcccctc agctattact ttttccgatt gaaacgacat attcatagtt tggttaggtt
     4381 gtaaaaatgc ggcaagttac aaaaaaatga caaatagcca attaccatca acctgcatgc
     4441 agcccatggt gaacttttaa aatggcaacg ccgttgctcg aagtaagccc gttagcccaa
     4501 ttaatattta agctaaatga ccttaatttt tgatttgcag gttatagacc ggttatttgc
     4561 taaatttgct ccagcagatt ttacaacttt ggataggttt gaggaaatac taaagcagta
     4621 ctgggggtat aacattttcc gttcgattca gcgggacatt attgaatcgg tttacaatgg
     4681 aaatgataca ctagcgctta tgcccaccgg aggtggtaaa tctattactt ttcaagttcc
     4741 tgctcttgcc ctggagggaa cctgcctggt tattactccg cttattgcct taatgaagga
     4801 tcaggttgaa aatctgaaga aaagggatat tagggcggtt gccgtgcatt ctggacttac
     4861 ccggcatgag gttgatgtag ctcttgataa ctgtgcatat ggagatgtga agtttcttta
     4921 cctttcaccg gagagaacaa ccacggagct attccgcgaa aggctcagaa aaataaaggt
     4981 gagcatggtg gccgtagatg agtcgcactg catttcgcag tggggctacg attttcgtcc
     5041 ttcatacctg cgcattgccg agttgaggga gattatttct gagcctgttc cttttcttgc
     5101 tctcactgca acggctacac ccgaggtggt tgaggatatt cagcaaaagc tgaagtttcg
     5161 caatggcaag gtattcagca tgagttttgc cagggaaaat cttgtttacc tggttaggaa
     5221 tgtggaggat aagccaaagc acctgctcaa aataacgcat ggcattccgg gaagtggcat
     5281 tgtttacgtc cgtagccgga gtaaaacccg agaaattgcc cagatgcttc aaaaagaagg
     5341 catttcggcc gatttttatc atgcgggctt aaccatggag gtgcgtaatg ccaagcaaaa
     5401 cgactggcaa gttggcaaaa ccagggttat tgttgccacc aatgcttttg gcatgggcat
     5461 tgataagccc gacgtccggt ttgtagtcca tgttgatctt cccgattcgc ccgaagcata
     5521 cttccaggaa gccgggcgtg ccggccgaga tcaaaagttg gcctatgcgg
//
LOCUS       DAOOQX010000033         5568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_17621_length_5568_cov_3.169599, whole genome shotgun sequence.
ACCESSION   DAOOQX010000033 DAOOQX010000000
VERSION     DAOOQX010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17621_length_5568_cov_3.169599"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PLX53_01065"
     CDS             complement(<1..436)
                     /locus_tag="PLX53_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY71701.1"
                     /translation="MSKSGLRSFPRNFWTVITMEFFERGSYYSVMSILSVYLVLGVNE
                     GGLGFTKESVGVIKSVITPLLYILPIISGAIADRFGYRKILFFSFAVMSLGYLLTSFS
                     NTYATVFLSLILMVTGAGFFKPIISGTIARSTDSRNSSLGFGI"
     gene            complement(653..1189)
                     /locus_tag="PLX53_01070"
     CDS             complement(653..1189)
                     /locus_tag="PLX53_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71702.1"
                     /translation="MKKILLACLAAAVFLITCIAASAQEDLKLQKLEEKVKIAQEKLD
                     KADAKVNSADSLISAGKSIIQEAEVEVRALEAEKKQLDKAHFAELKPLEKQLRSKDKD
                     KANSAKEEIKASETKYKEDLKGWNASYKEALKKYEAGKKMVQKGEENMKKAKDARKEA
                     EATLKEAEQALSKAEKAK"
     gene            complement(1308..3287)
                     /locus_tag="PLX53_01075"
     CDS             complement(1308..3287)
                     /locus_tag="PLX53_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOY71703.1"
                     /translation="MKKFNLYMGAIVAGVLMFYSPARSQEFSKIVPKQLGEKITLEYA
                     ISGETPGQLFNITPYYSVDGGKTFKQPKSVKGNVGSNVPGGRNLIVVWDVLKDLEELN
                     SEVIFKLDAKTSTTKPLTDDFNQVHFSLESLNRTKDGNIELLLSITNNGPTRDLKIIN
                     GLVTITDFNKQKYDAQKGNIAEVQGNERYSTPTRTIKQGETVKSRFVFERIPAEMDRI
                     MRLDLGVELITDEKYGIDLKIGKLQFRDLPVSPAPTTGLKVKRDYSFQLTSQPARFDI
                     RKVEIADAIPPEIVVTSPADVPLAGKEATRGRPYAQSSIGMDDRRLRSLASGEEYATT
                     DSSMTVKGKASDESGIYQVLVNGFEAKVDSNGIFEASIPLEIGRNEIAIRAVDLRQNA
                     VEKKFFVLRKAPSGKVAKGETEELDLVFDQPKTAPKFYALIMGANDYQDNSITDLDNP
                     ISDATKLYNVLVNRYTFDPNNVVFLKDPTREQMINALDMLTRRIGKNDNLLIFYAGHG
                     YWDKETDFGYWIPTDSKGNSTANWLANSQIKDYVAAIKSKHTLLIADACFGGSIFHSR
                     KAFQEGSSSTKEAFDAPSRKAMTSGNLTEVPDRSVFLEQLVTRLDGNTKNYVTAEEIF
                     ASIKNVVMSSSPVTPVYGEIKDAGDEGGDFIFVRK"
     gene            complement(3355..4401)
                     /locus_tag="PLX53_01080"
     CDS             complement(3355..4401)
                     /locus_tag="PLX53_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71704.1"
                     /translation="MVKFYLSSSLVLIFNMVIPFSGRSILAQDWNKFEGLFIQDTTQK
                     VKDTSNCAFKNISTLSYYPDTLPGWFFSPPVLYQGSAGLGISDPDMPMEKAREQAYLR
                     AKSMALLNYNARVQYYRDIYSSANEVGRYTNLRQRFDTYFKISSLATVSNAMFEVVDT
                     HFTRYNEYVVLLSYNPGNHSDDSLYKLTSVGSAFLVEATFDDVVDEQAEYELLCNATF
                     GEDSAASSHFLFREKGKRFLTSSFMNNAEVGYPMFPYSYVSPQDSARQHPFISYSGLW
                     SSYLRQLVNYLTLNAQQSSIKLKSMDDQYSTKLTNLSREIASFRASLNINGIKFANDT
                     LKLDLQLNEVESNF"
     gene            4773..>5568
                     /locus_tag="PLX53_01085"
     CDS             4773..>5568
                     /locus_tag="PLX53_01085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY71705.1"
                     /translation="MISICIPVYNFDVTELVDELLRQANHLNYPVEVLIFDDHSQQYF
                     RRQNSLLASRSKTSYLEFDFNIGRSKIRNRLADFAHGDWLLFLDCDVMVDSPDYLKTY
                     IDNLGRAKVLCGGREYGPKPFRPELLLRWKYGVSRECKSAFVRQTDPYSSFISGNFVI
                     SADIFNAIRFNEALSGYGHEDTLLGIELKRHNIETLHLDNAARHLGLDPCGEFISKTE
                     QGIVNLVRILNISAGNRREIEKSIKLLRIFRFLRHIGMLYPLRWVFK"
BASE COUNT         1430 a         1215 c         1287 g         1636 t
ORIGIN      
        1 atattccaaa ccccaacgac gagttccggc tgtcggttga tcgagctatg gttcctgaaa
       61 tgataggctt aaaaaacccg gctccggtaa ccattagaat taagctaagg aaaacggtag
      121 cataagtatt gctgaagctg gtaagcaggt aaccaagact catcactgca aatgaaaaaa
      181 agagtatttt cctgtatcca aacctatcgg caatggcgcc cgagattatt ggcaggatgt
      241 agagcagggg ggtaataacg cttttaatga ctccaacgct ttccttggtg aaaccaagcc
      301 ctccctcgtt aaccccaaga acaaggtaaa cggaaagtat tgacatcacg ctgtagtagg
      361 agccccgctc aaaaaactcc atggtgatta ctgtccagaa attgcgtggg aatgaacgta
      421 atcctgattt actcataaaa tatggtatta aagaagaaaa atcgaaatgt tatgggggtg
      481 aaagtagtaa aaaaataact ggataagcta atcgtatggg cttattcccg gatttcaggc
      541 ataaaaaaaa gggaccctcc tacgggtccc tccactggtg tgtttataga cgaaacaaac
      601 tatagagcct ttataaatag gattaaaaat caaatacagg agttgtaaag ggctacttcg
      661 ctttttctgc cttggaaagg gcctgttctg cttcctttag ggttgcttca gcttcctttc
      721 gagcatcctt tgccttcttc atgttttctt cccccttctg aaccatcttc tttccagctt
      781 catacttttt taaagcttcc ttgtagctcg cattccagcc tttcaggtct tccttatact
      841 tggtttctga agctttaatt tcctccttgg cgctatttgc cttgtcctta tcctttgatc
      901 gaagctgttt ttcaaggggt ttaagctcgg caaaatgggc cttatcgagt tgtttctttt
      961 cagcttcaag agctctaacc tcaacctcgg cctcctggat gatgctctta cccgcgctaa
     1021 tcaggctgtc agcagagttg acctttgcat cggccttatc gagtttttcc tgggcaatct
     1081 tgactttttc ctccagcttc tggagtttta aatcttcctg tgcagatgct gcaatgcagg
     1141 ttattaagaa tactgcagca gccaaacaag cgagtagtat tttcttcata ggagtaaaat
     1201 tttgctagtg ttcggtaaat gtttaccagc tataactacg aaattttatg tttagagttt
     1261 cggtaaaaaa cattaagggc tacaccatgt tcgggtagcc ctgatgctta ctttcttaca
     1321 aatataaaat caccaccttc gtcacctgca tcctttattt ctccatatac cggtgttacc
     1381 ggactgctgc tcatgacgac attctttatg ctggcaaaaa tttcctcggc agttacgtag
     1441 ttcttggtgt ttccatcaag tcgggtaacc agctgttcga ggaacacgct cctgtcggga
     1501 acttcggtaa ggtttccgct ggtcatagcc ttacggctgg gcgcatcaaa agcttccttg
     1561 gttgagctag aaccctcctg aaacgccttg cggctatgga aaatgctacc accaaagcag
     1621 gcatctgcaa taaggagggt gtgtttcgat tttatagctg caacgtaatc ttttatttgg
     1681 ctgttggcca gccagttggc cgtgctattc ccctttgaat ccgtgggtat ccagtagcca
     1741 aaatcagttt ccttgtccca gtatccgtgt ccggcgtaga aaattagcag gttgtcattc
     1801 ttaccaattc gcctggtgag catatcaagg gcattaatca tctgctcacg cgttgggtct
     1861 ttaaggaaaa caacgttgtt gggatcgaag gtgtagcggt ttaccagcac gttgtaaagt
     1921 ttagtggcat cactaatagg attatccagg tcggtaatgc tgttgtcctg gtagtcgttt
     1981 gcgcccataa tcaaggcgta gaacttaggt gcagtctttg gttggtcaaa aaccaagtca
     2041 agttcttcgg tttctccctt ggctaccttt cctgaagggg ctttccgtag aacgaaaaat
     2101 ttcttttcaa cggcattctg tctgagatca accgcgcgta tggcaatttc gttgcggcca
     2161 atttccaggg gtatgctagc ctcgaaaatt ccgttggagt caacctttgc ctcaaaaccg
     2221 ttaaccagta cctggtaaat tccgctttca tccgatgcct tgccctttac ggtcatgctg
     2281 ctgtcggtgg tggcatactc ttcacctgaa gccaatgatc taagccgtcg gtcatccatt
     2341 ccaatggatg actgggcata cggacgaccc cgggtggctt ccttgccggc caacggaaca
     2401 tctgcggggg aggtaaccac aatttctgga ggaatagcat cggcaatttc aaccttacga
     2461 atatcgaaac gtgccggttg ggaggtcagc tgaaagctgt aatcccgctt aaccttaagg
     2521 cctgtggttg gggctggcga tacggggagg tcgcggaact ggagctttcc aatctttaag
     2581 tctattccat acttttcgtc ggtaataagc tcaacgccca ggtcgagtct cataatccta
     2641 tccatttcgg caggaatgcg ctcaaaaaca aaacgcgatt ttacagtttc gccctgtttg
     2701 attgtacggg ttggggttga gtaccgctcg ttgccctgta cctcagcaat gttccctttt
     2761 tgggcgtcgt acttttgctt gttgaagtcg gtaatggtaa caagcccatt gataatttta
     2821 aggtcgcggg ttggtccgtt gttggtaatt gagagtagta gctcgatgtt accatccttg
     2881 gtgcggttca gactttcaag cgagaagtgt acctgattga agtcgtcggt cagtggtttg
     2941 gtggtgctgg tttttgcatc aagcttaaaa attacttcgg agttgagctc ttccaaatcc
     3001 ttaagaacat cccaaaccac aatgaggttt cttccaccgg ggacattgct accgacgtta
     3061 cccttgacac ttttgggctg cttgaaggtt ttgcccccat caacggagta gtatggggta
     3121 atgttaaata gctgaccagg ggtttcgccg gaaatggcgt actccagggt tattttctca
     3181 cccagctgct ttgggacaat tttgctgaac tcctgcgagc gagccggcga gtagaacatg
     3241 agaactccgg ctacgatagc tcccatgtag aggttaaatt ttttcatcac aacaggtagt
     3301 taatatgata caatttactt taaaaatttt taatccgaaa ggtgaaaatc aaagctaaaa
     3361 atttgattcg acttcattaa gctgcaaatc aagttttagt gtatcattag caaatttaat
     3421 gccgtttatg ttgagcgaag ccctgaagga ggcaatctcc ctgctaaggt tggtaagctt
     3481 ggtgctgtac tggtcgtcca tgctttttag tttaatgctg ctctgctggg cgttaagggt
     3541 taaatagtta acaagttgcc tcaggtagct gctccaaagg ccagagtagg aaataaaggg
     3601 gtgttgccgg gcggagtcct gaggagatac ataagagtag ggaaacatgg ggtaacctac
     3661 ctctgcattg ttcatgaagg agctggttaa aaatcttttg cccttttccc ggaaaaggaa
     3721 atgggagcta gctgcggagt cctctccgaa ggttgcattg cagagaagct catactctgc
     3781 ctgttcgtca actacgtcat caaaggttgc ctcaaccagg aatgcagagc caacgcttgt
     3841 aagcttgtaa aggctatcat ccgaatggtt cccggggttg tagcttagca ataccacata
     3901 ctcgttgtaa cgggtgaaat gggtatcaac tacctcaaac atggcgttac taaccgtggc
     3961 caacgatgaa attttgaagt atgtatcgaa ccgttggcga agattggtgt acctgcccac
     4021 ctcgttggcc gatgagtata tatccctgta gtactgaacc ctggcgttgt aatttaacag
     4081 tgccattgat tttgccctga ggtaagcctg ctctcttgct ttctccattg gcatgtcggg
     4141 atcggatatt cccaaacccg cagatccttg gtacagaaca ggagggctaa aaaaccatcc
     4201 gggaagggtg tcggggtagt agctaagggt gctgatattt ttaaatgcac aatttgaagt
     4261 gtccttaacc ttctgcgtgg tgtcctgaat gaataatccc tcaaacttat tccagtcctg
     4321 ggcaagaata ctccttcctg aaaatggaat taccatgttg aaaatcagca ccaggctgct
     4381 tgataggtaa aatttaacca tactccacta taggttttaa ataaaacaca gaaatctagc
     4441 cctaaaaatt aagtatttaa gataattttt tacctaaaag gtgttttgtt ccgtgaacca
     4501 tcaatttaca ttgatatatc cgaattgctt tttgctccat tttgccattc attttgaagt
     4561 gctgtgccag tgacttttgt gcactgctct tttgatatgg atgatgcgac atttggtcaa
     4621 aacaagcata cataagctgt taacaataga atttactgtc cttaggtata gaaagaaatt
     4681 tagccgaatt attggaattg gtaccccaac tctccttatt tttttattaa ttgcgttcaa
     4741 aattatggtg ttgctggtta agctaaacga tcatgatttc catttgcatt ccggtatata
     4801 attttgatgt aaccgagctg gttgatgagc tgctccgaca ggcaaaccat ctcaactacc
     4861 cggtggaagt gttaattttc gacgaccatt cgcagcagta cttccgccgg cagaattcac
     4921 tgctggcctc tcgtagcaaa accagctatt tggaattcga tttcaacata ggcaggtcga
     4981 aaataaggaa tcggttggcg gacttcgccc acggagactg gctgttattt ctcgattgcg
     5041 atgttatggt tgatagcccc gattacctaa aaacctatat tgataacctt ggcagggcaa
     5101 aggttctttg tggtggtcgg gagtatggcc ccaagccatt cagaccggaa cttcttctcc
     5161 ggtggaagta tggcgtcagc agggagtgca aatcggcctt tgttcggcaa accgatccgt
     5221 acagctcatt tattagcgga aattttgtaa tatcagctga tatttttaac gccataaggt
     5281 tcaacgaggc gttgtcgggc tacggacatg aggacacgct gctgggcatt gagctcaaaa
     5341 ggcataacat tgaaactctt catctcgata acgctgctcg gcacctgggg ctcgatccat
     5401 gcggcgagtt tatcagcaaa acggaacagg gaatagttaa cctggttagg atacttaata
     5461 tttcagcagg gaaccgtagg gaaattgaaa aatccataaa gctgctcagg atatttaggt
     5521 ttttgcgcca cataggcatg ctttacccat taagatgggt ttttaagg
//
LOCUS       DAOOQX010000034         5559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_17655_length_5559_cov_3.346839, whole genome shotgun sequence.
ACCESSION   DAOOQX010000034 DAOOQX010000000
VERSION     DAOOQX010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17655_length_5559_cov_3.346839"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PLX53_01090"
     CDS             <1..773
                     /locus_tag="PLX53_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOY71706.1"
                     /translation="FYDEGLKLVTFAPILKHGIFPLAEIPTDLLSIGQKEMNNHIEIE
                     FALNMSPRGGTPKMFNFLQIRPIVDTDQSEKFNWEAVDFSKAIIFSNSALGHGLVKNI
                     QDFVYVKPDRFNPAKTIEIAREVEEINNKFIELQRNYVLVGPGRWGSSDPWLGIPIKW
                     SQISEARVIVESGLDSFRVDPSQGTHFFQNLTSFGVAYLTINPFLNDGIYDIEFLNAQ
                     PAFLETTFCRWVNFSKPLTIQVDAKNNKGIILKSNGNS"
     gene            942..2279
                     /gene="gdhA"
                     /locus_tag="PLX53_01095"
     CDS             942..2279
                     /gene="gdhA"
                     /locus_tag="PLX53_01095"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOY71707.1"
                     /translation="MEVSKLMAELEKKHPGEVEYLQAVREVLESIEEVYNQNPQFESA
                     KIIERLVEPDRIFTFKVPWTDDEGKIHVNLGYRVQFNNAIGPYKGGLRFHPSVNLSIL
                     KFLGFEQIFKNSLTTLPMGGGKGGSDFNPKGRSDAEIMRFCQSFMLELWKYIGPETDV
                     PAGDIGVGGREIGFLYGMYKKLAHENTGVLTGKGLNWGGSLIRPEATGFGAIYFVQEM
                     LATRNIKLNGKTVALSGFGNVAWGAALKATELGAKVVTISGPDGYIYDEAGVSGEKIN
                     YMLELRNSNQDIVAPYAKKFPGSKFVAGKKPWEVKVDIALPCATQNELNGDDAALIIK
                     NGSICVAEVSNMGCTPEAVEAFTKAKFLFAPGKAVNAGGVATSGLEMTQNSMKLNWPK
                     EEVDVRLHQIMKDIHNACVKNGTGKDGYVNYVKGANIAGFLKVANAMLDQGLV"
     gene            2379..2792
                     /locus_tag="PLX53_01100"
     CDS             2379..2792
                     /locus_tag="PLX53_01100"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOY71708.1"
                     /translation="MSTTSTIIYQGNLRTVATHTRSGEQISTDAPVDNQGKGEFFSPT
                     DLLATSLGACMVTIMGISAQTHGFNIDGTRLEVQKIMGTNPRRVVEVIIDLYFPSDYS
                     DKHKRIIEAASRECPVAQSLHPDLKQTIRFHYAEN"
     gene            complement(2949..3827)
                     /gene="fabD"
                     /locus_tag="PLX53_01105"
     CDS             complement(2949..3827)
                     /gene="fabD"
                     /locus_tag="PLX53_01105"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOY71709.1"
                     /translation="MKAYVFPGQGAQFVGMGKDLYENSPAAREMFEKANAILGFRITD
                     LMFSGTDEDLRQTKVTQPSIFLHSVILAKVLGDEFSPNMVAGHSLGEFSALVAAGALS
                     FEDGLRLVAARAMAMQKACEKEPSTMAAVLGLDDAKVEEICRSIDEVVVPANYNCPGQ
                     LVISGSVKGVEIACQKLTEAGAKRALKLAVGGAFHSPLMEPARQELEAAIMSTPFSKP
                     ICPVYQNVTAMPTTNPDEIRKNLIAQLTSPVRWTQTVQNMIADGATSFVELGPGNVLQ
                     GLIKKIDRSVVAESRQ"
     gene            4278..5048
                     /locus_tag="PLX53_01110"
     CDS             4278..5048
                     /locus_tag="PLX53_01110"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOY71710.1"
                     /translation="MKTKEEIVNNWLPRYTGQAVEDFGTHILLTNFRGYLDYFAKFMG
                     VSIINPDANMPCATAKGITMIDFGMGSSNAATIMDLLSAVKPKAVLFLGKCGGLKRKN
                     QVGDFILPIAAIRHEGTSDDYLPPEVPALPAFNLQRVVSSTIRNHNRDYWTGTVITTN
                     RRVWEYDDDFKDYLRKIRAMAIDMEAATIFTVGFYNEIPSGALLLVSDQPMISEGVKT
                     IESDRVVTDLYLEEQVQIGIESLTRLINNSESVKHLRF"
     gene            5062..>5559
                     /locus_tag="PLX53_01115"
     CDS             5062..>5559
                     /locus_tag="PLX53_01115"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOY71711.1"
                     /translation="MAKSTTLDSYTKILEEFKNKIYKPIYLLSGEEHYYIDKLSDYLA
                     DNVLSATEKDFNLTIVYGRDVTIADVINTARRFPMMSNYQVVIVKEAQNIKNLSDLEI
                     YLKAPQPSTILVICNKLKPDGKSSSAKLTKLIGLVKQKGVYFESKKLYDYQVADWIVQ
                     YLKPKG"
BASE COUNT         1511 a         1303 c         1288 g         1457 t
ORIGIN      
        1 ggttctacga cgagggcctt aagctggtta cctttgcccc cattctgaag catggaattt
       61 tcccccttgc cgagattcct accgacctgc tcagcatcgg gcaaaaggag atgaataacc
      121 acatcgaaat agagtttgcc ctcaacatga gcccccgagg aggcactcca aaaatgttca
      181 acttcctcca aattcgcccc atcgtggata ccgatcaatc ggagaaattc aactgggaag
      241 cggttgactt ttccaaggca ataatcttct caaattccgc tcttgggcat gggttggtga
      301 aaaatattca ggattttgtt tacgtaaaac ccgaccggtt taacccggct aaaactatag
      361 agattgcccg tgaggtggag gaaatcaaca ataaattcat cgagttgcaa cgaaactacg
      421 tgctggtggg cccgggcagg tggggcagca gcgacccttg gctgggtatt ccaataaaat
      481 ggtcacaaat atcggaagcc agggttattg ttgaatccgg cctcgacagc ttcagggttg
      541 atcccagcca gggaacacat ttcttccaga accttacctc ttttggtgtt gcctacctca
      601 ccattaaccc attcctcaat gatggtattt acgatataga gtttctcaac gcacagccag
      661 ccttccttga aactaccttc tgccggtggg tgaatttttc taaacccctt accatacagg
      721 ttgatgcaaa aaacaataag ggtatcatct taaaatcaaa tgggaattcg taagttttaa
      781 atgttaacga tttgggtaaa aatctgctta atatcatatt tttaaacccc tatttctttt
      841 gaaatattat ttttttacat catttttgaa aaacttttaa ggggatataa atgatgccct
      901 ttcatagatt gttttgagta gtaccttaaa aaaattaagc aatggaagtt agtaaactaa
      961 tggcagagct tgagaagaaa cacccgggtg aggtggagta cttgcaagca gttcgtgaag
     1021 ttctggaatc aattgaggag gtttacaacc agaaccccca atttgagtcc gctaaaatta
     1081 tcgagcgttt ggtagagccc gatcgtattt ttacctttaa ggttccctgg acagatgacg
     1141 aggggaagat tcatgtaaat ctcggttaca gggttcaatt caacaacgcc attggcccct
     1201 ataagggtgg attgcgcttt catccaagtg taaacctttc aattcttaag tttttaggat
     1261 tcgagcagat ttttaaaaat tctctcacca cgctgccaat gggcggtggt aagggaggta
     1321 gcgacttcaa ccctaaaggt cgaagcgatg ccgaaattat gcgtttttgc cagtctttca
     1381 tgcttgagct ctggaagtac ataggccccg aaaccgatgt tccagccgga gacataggtg
     1441 ttggcggtcg cgaaattgga ttcctctacg gaatgtataa aaaacttgcc catgagaata
     1501 ctggtgtgct cactggtaaa ggtctaaact ggggaggttc gctaatccgt cccgaagcta
     1561 ccgggtttgg tgcaatttac tttgttcagg aaatgcttgc caccaggaat atcaagttaa
     1621 atggtaaaac tgttgcgctt tcaggttttg gaaacgtagc ctggggggct gccctaaagg
     1681 caaccgagtt gggagctaag gtggttacca tttctggtcc cgatggatat atctacgatg
     1741 aggccggtgt tagcggtgaa aagattaact acatgctcga gctgcgcaac tctaaccagg
     1801 atattgttgc accctacgcc aagaagttcc ctggctccaa gttcgtggca ggtaagaagc
     1861 cctgggaggt aaaggttgac attgcccttc cttgtgctac tcagaatgag ctgaatggcg
     1921 atgatgcagc tttaataatc aagaatggct ccatttgcgt tgccgaggtt tccaacatgg
     1981 gttgcacacc cgaggcagtt gaagccttca ctaaggctaa gtttcttttt gcccccggta
     2041 aggctgttaa tgccggcggt gttgccacct ccggcctgga aatgacccag aacagcatga
     2101 agcttaactg gcccaaggag gaggttgatg tccgtttaca ccagattatg aaggatatcc
     2161 acaacgcatg tgtaaagaac ggaacaggca aggatgggta tgttaactat gtaaaaggcg
     2221 ccaacattgc aggtttcctc aaggttgcca atgcaatgct cgaccagggc ttggtttaaa
     2281 ccagcttcct gtttggatga ttttactata aaagggctca actttgagcc cttttatgcg
     2341 tttatagcta cgaaagggta atcacaatct tacataatat gagcacaact tcaaccatta
     2401 tttaccaggg caacctaaga accgttgcca cacatacacg ttcaggcgaa cagattagta
     2461 ccgatgcacc ggttgacaac caggggaaag gggagttctt ctctccaacc gacctgctgg
     2521 ccacgtcgct tggcgcctgc atggttacaa ttatggggat ttctgcacaa acccacggct
     2581 ttaacattga cgggacgcgg cttgaagtgc aaaagatcat gggaaccaat ccccgtaggg
     2641 ttgtcgaggt tattattgac ctctacttcc caagtgatta ttccgacaag cataaaagaa
     2701 taattgaggc tgcttcgagg gaatgcccgg ttgcccaaag cctacacccc gatctaaagc
     2761 aaaccattcg tttccattac gccgaaaatt aaagagcttt aaacctaaag ggtaaaagcc
     2821 accagggttt ggcttgcggg agggtaaagg atatcgtaaa agactactgg tcaagaagcc
     2881 aagtttaggg gccaccttta ggtagcccct agttattatt tttcaaataa accttggaca
     2941 gcaaaggatt actgcctgct ttcggccacc acgctgcggt caattttctt gatcaacccc
     3001 tgaagcacgt tgcctggtcc cagctcaacg aatgatgtag ccccatcggc aatcatgttc
     3061 tggacggttt gtgtccatct taccggggag gttagctggg caatcaggtt tttcctgatt
     3121 tcatcagggt tggttgtagg cattgctgta acattttggt aaacagggca tatcggtttg
     3181 ctaaacggag tgctcatgat ggccgcttcg agctcctgcc gggccggctc cataagggga
     3241 gaatggaatg caccacccac tgccagctta agtgcccgtt tagcacccgc ttcggtaagc
     3301 ttctggcagg caatttcaac cccctttacc gatccggaaa taaccagctg tccggggcag
     3361 ttgtagttag ccggcacaac aacctcgtca atagagcggc aaatttcttc caccttcgca
     3421 tcatccagcc caagcactgc cgccatggtc gagggttcct tttcgcaagc tttctgcatg
     3481 gccatggcgc gtgcagccac cagccttagc ccatcttcaa agctcagcgc accggcagct
     3541 accagggccg aaaattcgcc aagtgaatga ccggcaacca tgttggggct aaattcatcg
     3601 cctaaaactt tagccagaat taccgagtgt aggaaaatgg agggctgggt aaccttcgtt
     3661 tgtcttagat cctcgtcggt tccactaaac attaggtcgg taatgcgaaa gcctagtatg
     3721 gcgttggcct tctcgaacat ttcccttgct gctggagagt tctcgtagag atcttttccc
     3781 atccctacaa actgggcgcc ttgtcccgga aatacgtacg ctttcatccg tagaaaattt
     3841 taggtttgtg tttacacttt tacgttggtc aatacttagt cttttgagtt ttatgtgaag
     3901 ccaaaaccaa caaaaacgct tgtaatcggt aaattttatt ggttcgtttt gccgcttgtt
     3961 ttacattaat cctctaaaaa agttaagcgg aaggtattat gactgtattt ttgtcaagca
     4021 aaaataaatc cccctgggaa aattttcccg gctatgaccc atccatccgg ctgacaaaag
     4081 tagggcattt tttcagattg tcaagttagc cgcatgggta aacctgtaaa tggttaaaaa
     4141 acattgcttc atggtaataa tgccgttatg ctgccccaat aaaattttta aactactttt
     4201 gcatgaaagc cgaaatgaag aggcctaaat gagtagcgct ccatcaacat acttttaacc
     4261 ctaataacga ttacggaatg aagaccaagg aggaaattgt aaataactgg ttgccccgct
     4321 atactggcca ggcggttgag gattttggta cccatattct tttgaccaac ttcaggggtt
     4381 atttggatta ttttgccaaa tttatggggg tttcaataat aaatcctgat gccaacatgc
     4441 cctgtgccac cgcaaagggg ataaccatga ttgactttgg catggggagt tcaaacgcag
     4501 caaccatcat ggatctgctt agcgctgtta agccaaaagc cgttcttttc cttggaaaat
     4561 gcggcgggtt gaaaaggaag aaccaggttg gcgacttcat cctacctatt gccgccatca
     4621 ggcacgaggg cacatccgac gactacctgc cccccgaagt gcctgccctg cctgcattca
     4681 accttcagcg ggtggtttcc tcgaccattc gcaaccacaa ccgcgactac tggacaggca
     4741 cggttattac caccaaccgt agggtttggg agtacgacga tgattttaag gattaccttc
     4801 ggaaaattag agccatggcc atcgacatgg aggcagcaac catatttacg gtgggctttt
     4861 acaacgagat tccttcgggt gccctgctgc tcgtttcgga ccagcccatg atttccgagg
     4921 gggttaaaac tatcgagagc gacagggtgg ttaccgacct ttaccttgaa gagcaggttc
     4981 aaatcggcat tgaatcactt acccgcttaa tcaacaacag cgaatccgta aagcatcttc
     5041 gtttctaacc tttgtccggc aatggctaaa agcaccaccc tcgattccta cactaaaatt
     5101 ctggaggagt ttaaaaataa aatttataag cccatatacc tcctatcggg cgaagagcac
     5161 tactacatcg acaagttatc cgactacctt gccgacaacg tgctgtccgc aaccgaaaaa
     5221 gattttaacc ttaccattgt gtacggcagg gacgttacca ttgccgatgt catcaacacc
     5281 gccaggcgat ttccgatgat gtccaactat caggtggtga tagtgaagga agcccagaac
     5341 attaagaacc tgagcgatct ggaaatttac ctaaaagcac cccagccctc aactattctg
     5401 gtaatctgca acaagcttaa gcccgatggg aaaagcagct ctgccaagct aaccaaactc
     5461 attggattgg ttaagcagaa aggggtgtac tttgagtcga aaaaacttta cgattaccag
     5521 gtggccgact ggattgtcca gtacctgaaa cccaaaggc
//
LOCUS       DAOOQX010000035         5558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_17660_length_5558_cov_2.840269, whole genome shotgun sequence.
ACCESSION   DAOOQX010000035 DAOOQX010000000
VERSION     DAOOQX010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17660_length_5558_cov_2.840269"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2053)
                     /locus_tag="PLX53_01120"
     CDS             complement(<1..2053)
                     /locus_tag="PLX53_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY71712.1"
                     /translation="MKHDSTTTVWDKYSCSKGWQFQLTKAAKIVALASALMLFFPIIT
                     HAQNSITGTIKDKATGDPLAGVHVSVGQQVATTNSQGFYVLKNLKPGTHIVAASLVGY
                     KSEQQPAKIVNESITLNFFLEEDLIFLNEVTVSATRTESRIADIPGRIEMITPEKISL
                     ASYQSVDELLTLIPGVHTARSFGLFSYRSSVSMRGVSSKEQGRTLVLLDGIPMNKADG
                     GSVNWNLISTREIERIEVVKGPGSALYGGNAMGGIINVVTQKPTRPLEGSVAGSYGTY
                     NTKGVNAKLAGKLNNGFYWAADGLFRNSDGYITQSIADRLTNPYIVKSTFEEKVLHLR
                     TGYDNGQKLQADVDFTLFDDVRTTGEKVYQPQGNTREYDTYQLRANVKGVAGAVVWNV
                     SAFFLNEDYKRVSEWMKDDYTWYDVLSVRRDYGLLTSANYRAGRHTFTGGVDLRMGSV
                     DASDVYYTSTDRVDNNGKMNFYGFYLQAVVALFDSTVNVTAGLRYDYARFFDGQFQIL
                     SPSAETAFMSQYQFSQNGSESWGALSPRISAQFKPNGNYRIYASYSRGFRPSVLDDLC
                     RSGRVKGGFKVANPNLKPEYLDNFEVGGDYKPFDRLRLAASAYYSTGKDFLYYVSTGD
                     SIDMGFGPRPIMIPSNISDVEIYGMEAEFSATPWRFLTLSGGYAYALSKITGYKPIAA
                     ND"
     gene            complement(2187..3080)
                     /locus_tag="PLX53_01125"
     CDS             complement(2187..3080)
                     /locus_tag="PLX53_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71713.1"
                     /translation="MRKLTICLIAIFLLAACSEKESVDTPPSITFQTGSYTQDGEAVP
                     LGGKLTFGITANGGSEPLTNIRIQRVAGGNVITEVDKGLFVKNEDYPYVVNAVKSDAS
                     EEVWRFMAMNSKRDSVEITRTVLLGEGVAYGPIRHFPSVKIGMQNNTEYPHFLCLSTG
                     TTYSNEEVAGNEASIDLLAFVYQTSGIWSPTLNCPQYTTAPSYYPIVGSWTTRNSTLY
                     DYNAVDNNLVSLDEFNAATNDSLLVASFKPQSSSGNCKFCFTGKIVPFKTQLGKYGLI
                     RIIHADQVAEGYMELEIKIQE"
     gene            complement(3151..3921)
                     /locus_tag="PLX53_01130"
     CDS             complement(3151..3921)
                     /locus_tag="PLX53_01130"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71714.1"
                     /translation="MKTNLVKVALMAAAFVVGFSSCDDDDPTYTVTLAVNPSNAGTVT
                     GGGDYKKGDIIQLTATANENFYFVSWTEGTNELSTEATFDYTVGDKDVTLTANFNEKK
                     IVTLGAQSNTTFPGFLSVSEGATYNFNEASSHQAAVDIFCFYEVGNDIALASPGSNIT
                     GIFGGTETDPANWTTKNTTLFNQVTTLTVAQFDTIPNGDANIQTYYSADAARKKAKLL
                     IKDNIYAFKTQNGTYGLLKVIDVSVGADGFIKFEYKTK"
     gene            complement(4247..>5558)
                     /locus_tag="PLX53_01135"
     CDS             complement(4247..>5558)
                     /locus_tag="PLX53_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="decatenates newly replicated chromosomal DNA and
                     relaxes positive and negative DNA supercoiling; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOY71715.1"
                     /translation="IDELNEQIRGIDKEIKQVKYDLENLVDYAVAYYDNLLKKYGKGR
                     ERKTELRSFDSIEATSVVAANEKLYVNRSEGFVGTGLKKDEYICDCSDIDDIIVFLRN
                     GKYVVQKVQEKAFVGKDIIHVAVYRKNDRQTTYNVLYRDGLNGDIMMKRCTISGTTRD
                     KEYDITKGTAGSQILYFNANSNGETDILKVFLKPKPRLRNLILELDFSQLAVKGRDSQ
                     GNIFTRHAIHKIQVKEKLAGQVEAQKVWYDPETLRLYKEAKGNFLGEFMPGEKILIAN
                     SDGTYFVAGTEYSLRFNAPPLVVEKFNPNKIYSAVYFDGEQNYFYLKRFTFEPTEKIQ
                     PFIPDDGKSYLVELNSDLYPNLLVRFGGKHASHQPETIDVDAFIAAKSYRAKGKRIST
                     LNVKKVEFTEPLQKEASAFSTIAGNNNAQLEEEDDGGAAEAVGK"
BASE COUNT         1326 a         1267 c         1302 g         1663 t
ORIGIN      
        1 agtcgttggc tgcgatgggc ttatatcctg taattttgga taaggcgtag gcatagcctc
       61 ccgatagggt gagaaacctc cagggagtag ccgaaaattc tgcctccatg ccgtatattt
      121 ccacatccga aatgttggag ggtatcatta ttggtcgagg tccaaacccc atgtcaatgc
      181 tgtcgccggt ggaaacgtag tataggaaat ctttgccagt ggaatagtag gcgcttgcgg
      241 ctaagcgtaa gcgatcaaaa ggtttataat cgccccccac ttcgaagttg tcgaggtatt
      301 cgggtttaag gttggggttt gctaccttaa aaccaccctt aacgcgtccc gagcggcata
      361 gatcgtcaag gactgaaggt ctgaaacccc tgctgtagct ggcgtatatt ctgtagttgc
      421 cgttaggttt gaattgggca gaaattcgtg gacttaatgc cccccagctt tctgaaccgt
      481 tctgggaaaa ctggtactgg ctcatgaaag ctgtttcggc tgaaggtgaa agaatctgga
      541 attggccgtc gaagaatcgt gcgtagtcgt acctcaaacc ggcagttaca ttcacggtgc
      601 tgtcgaaaag agccactaca gcttgcaggt agaaaccgta aaagttcatc ttgccgttgt
      661 tgtcaacccg atcggttgag gtgtagtaaa catcggaggc atcaacgctg cccattctca
      721 ggtcaactcc accggtaaac gtatgccttc ctgctctgta gtttgcgctg gtgagcaatc
      781 cataatcgcg acgaacggaa agcacatcgt accaagtgta gtcatctttc atccattcgc
      841 ttacgcgctt gtagtcctca ttcagaaaga atgccgaaac gttccacaca acggctccgg
      901 caactccctt tacgtttgcc cgaagctggt aggtgtcgta ctcacgtgtg ttgccctgcg
      961 gctggtaaac cttttcgcca gttgtgcgaa catcgtcaaa cagcgtaaaa tctacatcgg
     1021 cctgcagctt ctgcccatta tcatacccgg ttctcagatg cagcaccttt tcctcaaagg
     1081 tggatttcac aatgtatggg tttgtgagcc tgtcggctat tgactgggta atgtagccgt
     1141 cgctgttgcg gaataaccca tcggccgccc agtagaatcc gttgttcagc ttgcctgcca
     1201 gtttagcgtt cacccctttg gtgttgtagg tgccatagct gcccgcaacg ctaccctcca
     1261 atggacgggt aggtttttgg gtaacaacat taataattcc acccatggca ttcccaccgt
     1321 aaagggcgga acctggccct ttaaccacct ctatgcgctc gatttctctt gttgatatca
     1381 ggttccagtt cacagagcca ccatcggcct tattcattgg gattccatcg agcagcacaa
     1441 gggttcgtcc ctgctcctta gagcttacgc ccctcatcga aacggatgag cggtaggaga
     1501 aaagtccgaa tgagcgtgct gtatgcaccc caggaataag cgtcagcaac tcgtcaacgc
     1561 tctggtagga ggcaagcgat attttctcgg gggttatcat ctcaatacgg ccaggaatat
     1621 ctgctattcg gctttcggtt cgggtagccg aaacggttac ctcgttgaga aatatcaaat
     1681 cttcctcaag aaaaaagttg agggtgattg attcgtttac aatcttggca ggttgttgtt
     1741 ctgacttata ccctaccagt gaggcagcaa ctatgtgggt tccgggtttt aggtttttca
     1801 atacataaaa accttgcgag ttagtggtgg caacctgttg tccaaccgac acatgaaccc
     1861 ctgcaagggg atcgccggtt gccttatcct taatggttcc cgtgatggag ttttgggcat
     1921 gggtgatgat ggggaagaac agcatcaatg ccgaagctaa agctactatt ttagcggcct
     1981 tagttagttg aaattgccag ccctttgaac agctgtattt atcccaaaca gtagtagtgc
     2041 tatcgtgttt catcactaat ttttcctgtg ttataatttc acctttgttg atttttactt
     2101 ctatttctat gctttcctat ggaaataatt ttaccttccc ttttttatac aggtgatgcc
     2161 gtttaattag cagcaaccat tctcaactac tcctgaattt taatttccag ctccatgtat
     2221 ccttctgcaa cctgatcggc atgtataatc ctaataagac cgtacttgcc cagctgggtt
     2281 ttgaaaggga caattttacc ggtgaagcag aacttgcaat ttccgcttga actttgaggt
     2341 ttaaatgaag ccaccagcag gctgtcgttg gtagcggcat taaattcatc aaggctaacc
     2401 aggttgttat ccactgcgtt gtagtcgtag agtgttgagt ttcgtgttgt ccagctgccc
     2461 acaatggggt agtagcttgg tgcggtggtg tactgcgggc agttaagggt tggcgaccag
     2521 atgccgctgg tttggtaaac aaatgccagc aaatcaatgg aagcctcgtt gccggcaacc
     2581 tcttcattgc tgtaggtggt gccagtgctt aggcagagaa agtgtgggta ctccgtgttg
     2641 ttctgcattc caattttcac ggaggggaaa tgccttattg gtccgtatgc tacaccttca
     2701 cctaaaagca cggttcgggt aatttcaaca ctgtcgcgct tggaattcat ggccataaat
     2761 cgccacacct cctccgaagc atcagattta actgcattaa ccacgtaagg gtagtcttcg
     2821 ttttttacga ataacccctt atctacctca gtaataacgt tgcctccggc tacccgttga
     2881 attctgatat ttgtaagtgg ctcacttcct ccatttgcag tgattccgaa cgttagctta
     2941 ccgcctagag gaactgcttc accatcctgc gtgtagctcc cggtttgaaa agtaattgat
     3001 ggaggggtat ccaccgactc tttttcactg caagctgcta gcaggaaaat tgctattagg
     3061 caaatagtta gctttcgcat agttttaaaa aaaatagtaa ccccgaaaag tttgcttcct
     3121 ttcagggtta atgagtttaa aattaactgg ttattttgtt ttgtattcaa attttataaa
     3181 accatcggct cctacgctta catctataac ctttagcagt ccgtaggttc cattctgagt
     3241 tttaaaggcg tagatgttgt cctttataag caattttgcc tttttgcgag ctgcatcggc
     3301 actatagtaa gtttgaatat ttgcatcgcc gttggggatg gtgtcaaact gggcgacagt
     3361 aagagtggtt acctggttaa aaagggtggt gtttttggtt gtccagttgg cagggtcggt
     3421 ttcggttccc ccaaagattc cggtgatgtt tgagccaggg ctggccagcg ctatatcatt
     3481 ccccacctca tagaagcaaa aaatatcaac agctgcctgg tggcttgaag cttcgttaaa
     3541 gttataggtg gcaccttcgg aaacggaaag aaatcctgga aaagttgtgt tggattgtgc
     3601 acctagtgta acaatttttt tctcgttgaa attggcggtt agcgtaacat ccttatcccc
     3661 aacagtgtaa tcgaaggtgg cttcggtgct tagctcgttg gttccttctg tccagcttac
     3721 aaagtagaaa ttctcgttgg ctgtggcagt aagctggata atgtcgccct ttttgtaatc
     3781 ccctccaccg gtaacggttc ctgcgttgga tggatttacg gcaagggtta cggtgtaggt
     3841 tggatcgtcg tcgtcacaac tcgaaaaacc aacgacaaat gcagctgcca taagggcaac
     3901 cttaacaaga tttgttttca tacttttaat gctgtttttg agtagtttaa catttggttt
     3961 aatatgcgta tcaaatgcac ttttcctcga gtttaaaatc gtatttctac agctatcacg
     4021 gcctccctct tcatttcaac gttgatgagt tatgttctcc ttccttgtta agaggctagg
     4081 tgtctttttg ttatgaaaat tattacatat cgattggcaa aggtacatca ttattaaaaa
     4141 tttcaaaata aattgaaaag ttgcagcttg gtgtagctgg ctgcagggct aattggatgc
     4201 cagggtaaga actttgccat gtgagctagc atgtagcgct actttgctac ttaccaactg
     4261 cttcagcagc tcctccgtca tcttcttctt cgagttgtgc attgttattg ccggctatag
     4321 tgctaaaggc tgaagcttcc ttttgcaggg gttcggtaaa ctctactttt ttaacgttga
     4381 gggtggaaat tcgtttcccc ttagcgcggt aacttttagc tgctataaat gcatccacat
     4441 caatggtttc gggttggtga ctggcatgct ttccaccgaa tcgtaccagt aggttggggt
     4501 ataaatcgct gttcagctcc acaaggtacg acttgccatc gtcgggaata aagggttgga
     4561 ttttctcggt gggttcaaag gtgaaccgct taaggtaaaa gtagttttgc tcaccatcga
     4621 agtaaacagc cgagtaaatc ttgttggggt tgaatttttc caccacaagg ggaggagcgt
     4681 taaatcgcag ggagtactcc gttccggcca cgaaataggt tccatcgcta tttgctatca
     4741 aaattttttc gccgggcata aactcaccta aaaaattacc ttttgcctcc ttgtagagcc
     4801 ggagtgtttc ggggtcgtac catacttttt gggcttctac ctggccggct aatttttcct
     4861 tcacctgaat tttatgaatg gcatggcggg taaaaatgtt gccctgcgag tcccttccct
     4921 tcacagccag ctggctaaaa tccagctcaa gaatcagatt tcgaaggcga ggtttgggtt
     4981 tgaggaaaac ctttaaaata tctgtttcgc cgttggagtt ggcgttaaag tagagaatct
     5041 gtgagccagc tgttcccttg gtgatgtcgt actccttatc gcgggtggtg ccgcttatgg
     5101 tgcagcgctt catcataata tcaccattta aaccatcacg gtacagcacg ttgtaggtag
     5161 tttgcctgtc atttttcctg taaactgcca catggatgat gtccttgcca acaaatgcct
     5221 tttcctgcac cttctgaaca acatactttc cgttgcgcag gaaaacgata atgtcatcaa
     5281 tgtcggagca atcgcagatg tactcgtctt ttttaaggcc ggttcccaca aagccctcgg
     5341 aacggttaac gtatagtttc tcgttggcag caacaacgct ggtggcctca atgctgtcga
     5401 agctgcgaag ctcggttttg cgttccctgc ctttaccgta cttctttaac aggttatcat
     5461 aatacgccac agcataatca accaggttct ccaggtcata tttcacctgc ttaatctcct
     5521 tgtctatgcc tcttatctgc tcattaagtt catcaata
//
LOCUS       DAOOQX010000036         5512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_17819_length_5512_cov_3.831959, whole genome shotgun sequence.
ACCESSION   DAOOQX010000036 DAOOQX010000000
VERSION     DAOOQX010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17819_length_5512_cov_3.831959"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(282..2756)
                     /gene="lon"
                     /locus_tag="PLX53_01140"
     CDS             complement(282..2756)
                     /gene="lon"
                     /locus_tag="PLX53_01140"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOY71716.1"
                     /translation="MERNSRNFYSKLLIDDSNESNVNFIPILADDGTPELKDADIPDV
                     LPIMALRDTVLFPGIVMPITVGREKSLKLIKSLPPRKRMIGVVAQVDSKIPDPTAEDL
                     WHIGTVAQVIKVLEMPDRNFSVILKGICRFEVKNYLSEEPFFMARVSTLADILPDESD
                     KDFAAIASSIRDITIRIINLTPTLPQEAAFAIKNLDDNQLLVNFVSANSDISPEEKQK
                     LLEKSMLKERATQLLQYLTREVQMLELKQDIQSKVKLDLEQQQREYFLQQQIKTLQEE
                     LGANPTEQEVNDLKERAKQKKWSKEIAEHFDRELSKLNRMNPMAAEYSVQLNYVELLL
                     DLPWNEFTQDNFDLAHAREILDEDHFGLEQVKERILEHLAVLKLKGDLKAPILCLYGP
                     PGVGKTSLGKSVARALGRKYVRISLGGMHDEAEIRGHRKTYIGAMPGRIIQSIKKVNS
                     SNPVIILDEIDKVGQDFHGDPASALLEALDPEQNFAFHDNFLEVEFDLSKVLFITTAN
                     NIATISPALRDRMEMIEVSGYCMEEKVQIAKRHLVPNQITEHGLNSSQLQLNEKTIEE
                     IIKNYTRESGVRNLNQRIAKVVRNVAKKVAFEETYNPRLTIKEVRSILGVPRYNSDSS
                     IGNELAGVVTGLAWTETGGEILFVETSLSKGKGNLTLTGNLGDVMKESAVIALEYIKA
                     HANLLGINPDIFERWNVHVHVPEGAIPKDGPSAGITMVTSLASAFTQRKVKSSIAMTG
                     EITLRGKVLPVGGIKEKILAAKRANIMDIILPFENKKDVLEINSDYIKGVKFHYVGNI
                     MEALTFALLPEKVTNPICLEEPPRNN"
     gene            complement(2860..3447)
                     /locus_tag="PLX53_01145"
     CDS             complement(2860..3447)
                     /locus_tag="PLX53_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71717.1"
                     /translation="MEGALIRGPFSLGEHSAVKMGAKIYGPTTIGTHCKVGGEINNSV
                     IFGYSNKAHDGFLGNSVIGEWCNIGADTNNSNLKNNYAEVKLWSYADNAFTSTGLQFC
                     GLFMGDHSKCGINTMFNTGTVVGVCANIYGSGFPRNFIPCFSWGGACGFKTHQPEKAL
                     ETARLMMQRRNLELTQTDIDILKKIFDLTSQYRNF"
     gene            complement(3393..4049)
                     /locus_tag="PLX53_01150"
     CDS             complement(3393..4049)
                     /locus_tag="PLX53_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HOY71718.1"
                     /translation="MAAGFILFDDDRREALLPFTFTRPVSEIRIGILTITQKWERYLG
                     SSTSFLTTGYLSKKYPQNTTGERFCLINGGVCPDKALVNAIMSLEAGEVLVHENVVLA
                     GVMDKLPAETPSLSDFRVRIPYPNEVLQVKHLWDIFRLNGEALTRDFDMLTLNRKSAP
                     ISSSNRTLNPSRIFIEEGARVECSTLNAKPAPYISPRMPRLWREPLSGVHFRLASIPP
                     "
     gene            4283..>5512
                     /locus_tag="PLX53_01155"
     CDS             4283..>5512
                     /locus_tag="PLX53_01155"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOY71719.1"
                     /translation="MKRILSIFTAVILLSSAVFAQQKVIPLKGSKSAISVKQSTLQQL
                     RVSVSFSSLISNEVVTESGEKYVNLAIPDGFSTGLVGTPKLPAYKKLIQVPLGAKPIV
                     KVHSFSHVDIALGDYGINHPVIPVQPPVRKDQNLDSLKFIMKAEVYRKNEMIEPQLVK
                     VDMLGTMRGVQVARLEVNPVAYNPVTGMLRIYNDIDVEVDFEGASQAKFDEMFAKTSS
                     PYFETSYSFLINDLTKSSFDDHPDLTTYPVKMLIVSNRMFESTLVPFVEWKTRKGFNV
                     EVAYTDVIGNTAEAIKTFVHSSYTNATPENPAPTFLILVGDVAQVPASGVGSASYKQT
                     DLYYASVDGDYFPEMYYGRMSASTNVQLQNIIDKILYYEQYGFEDPTYLNSATLIAGV
                     DATWNSKVGQPTIKYGTK"
BASE COUNT         1344 a         1264 c         1270 g         1634 t
ORIGIN      
        1 cagtgccgct tgggtattcc cacgctacgt tccgcccccg acccatttgc gggtcgggct
       61 tttatgccgt ttttacttat accggcaacg ttcaacgttt cccgacttta tgagcaacgc
      121 ggaggatacc ttttctcccg ttagggctgg agaaaaggtt aaaggataag gtagacttgt
      181 ccgactgttg gtgaaacccg atgcagagtt ggtggattcg ccaaaattat tccgtcttct
      241 aagggcttta aattgttgct tgggaagagg tttatttatg cttaattatt acgagggggc
      301 tcctccagac aaatagggtt tgtaactttt tcgggaagca gtgcaaatgt caatgcttcc
      361 attatgttgc ctacgtagtg gaacttaacc cctttaatgt agtcgctgtt tatctccaga
      421 acgtcctttt tattttcgaa ggggaggatt atatccatga tattagccct tttggcagcc
      481 aggatttttt cctttatgcc tccaactggc agcaccttgc cacgaagagt aatctcaccg
      541 gtcatggcta tgctgctttt aaccttacgc tgggtgaagg ctgatgccag ggaggtaacc
      601 atggttattc cagcagatgg tccatccttg ggaatggcac cttcgggtac gtgaacatgc
      661 acattccacc tttcaaaaat atcagggttt attcccagca ggttggcatg agcctttatg
      721 tactcaagtg cgataacggc cgactccttc atcacatcac ccagatttcc ggtaagggta
      781 agatttcctt tccccttgct aaggcttgtt tctacaaaaa ggatttcgcc accggtttcg
      841 gtccaggcaa gtccggttac tactccggcc aactcgtttc caatggatga gtcgctgttg
      901 taccgcggaa cacccagaat agaccttact tcctttatgg ttaagcgggg attgtatgtt
      961 tcttcaaacg ccaccttttt ggcaacgttt cgcacaacct tggcaattct ttggttaagg
     1021 ttgcgtacgc cactttctcg ggtgtagttt tttattattt cctcaattgt tttttcgttt
     1081 agctgcagct gggaggagtt gagaccatgc tcggttatct ggttgggaac caggtggcgt
     1141 ttggcaattt gaaccttttc ctccatgcag tagccgctaa cctcaatcat ttccattcgg
     1201 tcacgcaggg caggacttat ggtggctatg ttattggcag ttgtgatgaa cagcaccttg
     1261 gataggtcga attcgacctc aaggaagttg tcgtgaaatg cgaagttctg ctccggatcg
     1321 agcgcttcca aaagggcaga tgcgggatcg ccatgaaaat cctgtcccac cttgtcaatc
     1381 tcatcaagaa taataaccgg attggatgag ttaacctttt taatgctctg tattatcctg
     1441 cccggcatgg ctccgatgta ggttttgcga tgccccctaa tttcagcctc gtcgtgcata
     1501 cccccaagtg aaatgcgcac gtactttctg cctagtgctc gtgcaaccga ttttccaaga
     1561 gatgtttttc caactcccgg agggccatac aggcagagta ttggggcttt aaggtcgccc
     1621 ttcagcttca aaacggccag gtgttcaaga atgcgctcct taacctgttc gagtccaaaa
     1681 tggtcctcat ccagaatttc tctggcgtgc gcaaggtcaa agttatcctg agtaaactcg
     1741 ttccatggca gatcgaggag cagctccacg tagttgagct gaacggagta ctcagcagcc
     1801 atggggttca tccggtttag cttgctgagt tcccggtcga aatgctcggc tatctccttg
     1861 ctccattttt tctgctttgc cctttctttc agatcgttta cttcctgttc tgtggggttg
     1921 gccccaagtt cctcctgcag ggttttaatc tgctgctgta ggaagtactc ccgttgctgc
     1981 tgctcaagat cgagctttac tttcgactgt atgtcctgtt ttaactccag catttgaacc
     2041 tcgcgtgtaa ggtactggag gagctgagtg gctcgctcct ttagcatgga tttttcaagt
     2101 agcttttgct tttcttcagg gctgatgtcg ctgtttgcgc ttacaaagtt gacaagtagc
     2161 tgattgtcgt ccaggttttt aatggcaaag gctgcctctt ggggtagggt gggggtgagg
     2221 ttaatgatcc ggatggttat atcgcgaata gagctggcaa ttgcagcaaa atccttgtcg
     2281 ctttcatctg ggagaatgtc tgcaagcgtg cttaccctgg ccataaaaaa gggttcttcg
     2341 ctgaggtagt tcttaacttc aaatcggcaa attcccttta gtattaccga aaaattccta
     2401 tcgggcattt cgagcacttt aataacttgg gcaacggttc caatatgcca caaatcttct
     2461 gcagttgggt cgggaatttt ggagtcaacc tgggcaacca ctccaatcat ccttttacgc
     2521 gggggtaggc tttttattag ttttagcgat ttttccctgc caaccgttat aggcataaca
     2581 atacccggaa agagaaccgt atcgcgaagc gccataatcg gcaaaacatc cggaatatca
     2641 gcatccttga gttcgggtgt accatcgtcg gcgagaatgg gtataaaatt cacatttgac
     2701 tcgttggagt catcaataag aagcttgcta tagaaatttc tgctatttct ttccatactg
     2761 atacctcaat gcctaatgac aacatgtcat tatctcgatt tattttttcg agtattgata
     2821 tgggcaatac ctatgccaaa aagttttaca tggttgcact taaaagtttc ggtactggct
     2881 tgtcaggtca aaaattttct ttagaatgtc gatgtcagtt tgggttagct ccaaatttcg
     2941 acgctgcatc attaaacggg cagtttccaa agctttttca ggttgatgcg ttttaaatcc
     3001 acacgcccca ccccatgaga agcagggaat aaaatttcgg ggaaatccgc tgccataaat
     3061 attggcgcag acacccacca ccgttccggt gttaaacatg gtgttgatgc cacatttgga
     3121 atggtcgccc atgaacaacc cgcagaattg aagtccggtg ctggtaaatg cgttatccgc
     3181 ataactccag agtttaacct ctgcgtagtt gtttttcagg ttggagttgt tggtgtcggc
     3241 accaatgtta caccattcgc caatcaccga gttccccaga aagccatcgt gggccttatt
     3301 tgagtaacca aatattacgc tgttgtttat ttcacctcca accttacagt gcgtgccaat
     3361 agtggtgggg ccgtaaattt tagcacccat cttcacggcg gaatgctcgc caagcgaaaa
     3421 tggacccctg ataagggctc cctccataac ctcggcatcc gaggcgatat atatggggcc
     3481 ggcttggcat ttagagttga gcattcaacc cttgcaccct cctcaataaa tatcctggat
     3541 gggttaagag tccggttact tgacgaaatt ggtgccgatt ttctgtttaa tgttagcatg
     3601 tcgaagtctc tggtcaaggc ttcaccgttt agccggaaaa tatcccagag atgtttcacc
     3661 tgaagcactt cgttggggta gggaattctg acacgaaaat ccgacaggga tggtgtttcg
     3721 gcaggtagtt tatccattac acctgctagc acaacatttt catgaactag cacttcgcct
     3781 gcttctaatg acattatggc attaaccagc gctttatccg ggcaaacacc cccgtttatc
     3841 aagcaaaatc tttcaccagt ggtgttttgt ggatactttt tcgaaaggta gcctgtggta
     3901 agaaatgatg ttgaactacc aaggtaacgc tcccattttt gggtaattgt tagaatgcca
     3961 attcgaattt ctgaaaccgg ccgggtaaac gtgaagggga gaagggcttc ccttctatca
     4021 tcatcaaata ggataaaacc agctgccata accttaaatt taagacaaac gtagttaaaa
     4081 aatcggtcgc ttgttagagg ttttctggct actatgaatt ttaccaagct gccggtcttg
     4141 gctgaactgc ttgattcccc tagttgctaa tttgtaaaac ttttttaggc aggacatgaa
     4201 agctctaaat tggcattttt taacataata gcttaacttg cacaatgttt gttgttatgg
     4261 ttgattcaat atttttcctg tgatgaaaag gatactatcc atttttaccg ctgtaattct
     4321 cttgagttct gctgtttttg cccagcaaaa agttattccc ttaaagggta gtaaatcggc
     4381 cataagcgtt aagcagagca ctcttcagca gctaagggtt tcggttagtt tcagctcgct
     4441 tatatcaaat gaagtggtaa cggaatcggg cgaaaagtat gttaacctgg ctattccaga
     4501 tgggttttcg accggacttg ttggcactcc caaactgcct gcctacaaga agctcattca
     4561 agttccacta ggggccaagc caatagtaaa ggttcacagt ttcagccatg ttgatattgc
     4621 gctaggtgat tacggaatta accatcctgt tattcccgtt cagcctcctg ttcgtaagga
     4681 tcagaatctt gactcattaa aattcatcat gaaggctgag gtttaccgta agaacgagat
     4741 gatagaacct cagctggtta aggttgatat gctgggaacc atgcgtggag ttcaggtggc
     4801 aaggcttgaa gtgaaccctg tggcctataa tccggtaacc ggtatgctta gaatatacaa
     4861 cgatattgat gttgaggttg actttgaggg ggctagccag gccaagtttg atgaaatgtt
     4921 tgccaaaact tcttcaccat actttgagac atcctacagc tttctaatca acgaccttac
     4981 caaaagttcc tttgacgatc atcccgactt gaccacctat ccggttaaga tgctaattgt
     5041 ttcgaacagg atgtttgaat caacccttgt cccctttgtt gagtggaaaa cgaggaaagg
     5101 gttcaacgtt gaggtggcct ataccgatgt cattggtaac acggccgaag caatcaaaac
     5161 cttcgtccat agttcctaca caaacgctac tcccgaaaac cctgcaccaa catttcttat
     5221 ccttgtgggc gacgtggccc aggttcccgc ctcgggcgtg ggttccgcct cttacaagca
     5281 aaccgacctt tactatgcaa gcgttgatgg cgactacttt cccgagatgt actacgggcg
     5341 catgtcggct agtacaaatg ttcagcttca gaatattata gataagatac tttactacga
     5401 gcagtacggg tttgaggatc caacctatct taattcggcc actctgattg ccggtgtcga
     5461 tgccacttgg aattcgaagg ttggtcagcc aaccataaag tacggaacca ag
//
LOCUS       DAOOQX010000037         5484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_17928_length_5484_cov_2.857432, whole genome shotgun sequence.
ACCESSION   DAOOQX010000037 DAOOQX010000000
VERSION     DAOOQX010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17928_length_5484_cov_2.857432"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLX53_01160"
     CDS             <1..496
                     /locus_tag="PLX53_01160"
                     /inference="COORDINATES: protein motif:HMM:NF025668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HOY71720.1"
                     /translation="PRSVGQIPIYYSMRSTGRPFDANNKYTSKYLDESNDPLYPFGYG
                     LSYTTFDYGEVELSSSEITETDTLDISVSVTNTGKYYGREMVQLYIHDNVASVAPPVK
                     LLKQFQFVDLKPGESKKMVFKLTVNDLMFYRDDMSYGFEKGQFTVYIGGNSRDVRTAF
                     FSLN"
     gene            complement(644..1351)
                     /locus_tag="PLX53_01165"
     CDS             complement(644..1351)
                     /locus_tag="PLX53_01165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOY71721.1"
                     /translation="MATYIISDIHGCIQTLRMLIEGKLKVSLQDRLFFLGDYIDRGPD
                     SSAVVEYIIGLMRSGYTISCLIGNHEQMMLDSVKRESELCLWQANSGQATIKSYVKMF
                     GFDDDGHLGFLPKAHLDFFTSLKQYFIVDDKYILVHGGLNTTSANPLEDTEAMLWSRL
                     DDAPNDFMQDYRIIHGHTPEALDVIADKLKQPNRRIFGLDAGCVYRGMLFPGTGYLVA
                     LDLDKWELNFVECLDKW"
     gene            1634..2059
                     /locus_tag="PLX53_01170"
     CDS             1634..2059
                     /locus_tag="PLX53_01170"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOY71722.1"
                     /translation="MPSGNNVVEHLGRVDAITNNDIRVVITSQSACESCHARGACLAS
                     ESKEKFIVVTIPNHNFIVGEPVKVLLKESLGFRALFLGYVLPLLVVLTTLFTLSAAGM
                     SEKDAGLLSLAVLAPYYLGLYIFRKKIARKFEFGIEKIS"
     gene            2072..2938
                     /locus_tag="PLX53_01175"
     CDS             2072..2938
                     /locus_tag="PLX53_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HOY71723.1"
                     /translation="MSSVLTTSILMLTILGVVLAVILYVVAQKFKVEEDPRIDEVEAL
                     LPGANCGGCGYPGCRGLADALVKADDISAISCPVGGNETMRKVAAILGKAVEDKDPQI
                     AVVRCSGSPQNRTRVVEYNGISSCKFASTLYSGDTGCQFGCLGLGDCVAVCKFDAIYI
                     DPVSLLPVIDEEKCTACGACVKACPKLLIELRKKGPKGKRIFVSCMNTDKGGVARKYC
                     SVACIGCFKCVKVCPYEAITLENNLAYIDANKCKLCRKCVEECPTDSILEVNFPPRKP
                     KVEEQAQNAVTS"
     gene            2981..4312
                     /gene="rsxC"
                     /locus_tag="PLX53_01180"
     CDS             2981..4312
                     /gene="rsxC"
                     /locus_tag="PLX53_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOY71724.1"
                     /translation="MLKTFKKGGVHPPENKISAAQPVEVLPLPAKVIIPLSQHIGAPA
                     EAMVAKGDRVKTGQLIAKSAGFVSANVHSTVSGIVESLEPVMDSSGYKRPAITINVEG
                     DDWEPSVDRSPNLKKEITLSAQDIIKKIAEAGIVGLGGATFPTHVKLSVPAGKKAEML
                     VINGVECEPFLTSDHRLMLEKGHELMIGIQILMKALNVPKAMVGIEANKPDAVSHLQS
                     LAKSYSGIEVQPLKVKYPQGGEKQLIKALTGREVPSGGLPIDIGAVVHNVGTALAVYE
                     AVQKNKPLVERIVTVTGKNLAHPSNFLVRIGTPVSALIAAAGGLPDDTGKVVSGGPMM
                     GKAVSSIDVPVTKGTSGILVIPAAESKRKPEGVCIRCAKCVSVCPMGLEPYLLYRLGQ
                     RMQYSQMEVERVLDCMECGSCSFECPANLFLLDFIRLGKVEVGKIVRERKN"
     gene            4350..5354
                     /locus_tag="PLX53_01185"
     CDS             4350..5354
                     /locus_tag="PLX53_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOY71725.1"
                     /translation="MDNRFVVSPSPHVHSGKSTSQLMLNVVLALMPAFAVSIYYFGVG
                     AIIVTLISVLSCVLFEYLIEKFIFKVKPTISDCSAVVTGLLLAFNVPSNLPRYIIVLG
                     SLFAIGVAKMSFGGLGNNIFNPAIAARIFLLISFPVQMTSWPLPVTSRLQYIDAITGP
                     TPLAIIKEGVKNGQTISQMADSIPSHMQLFMGLMGGSLGEIAAFALLIGFAWLLITRV
                     ITWHIPVSIFTTIFVFTGILWLINPGIYADPVFHLLTGGVMLGAIFMATDYVTSPMHP
                     KGMWIYGIGIGALTVIIRVWGSYPEGVSFAILIMNAFVPLINRFVKPKRFGAAAISGK
                     "
BASE COUNT         1405 a         1160 c         1343 g         1576 t
ORIGIN      
        1 cccgcgttcg gttggacaaa tccccattta ctactccatg cgaagcaccg gtcgcccttt
       61 cgatgcaaac aataagtaca cctcaaagta cctcgacgaa agcaacgatc cgctctaccc
      121 ttttggctac ggattaagct acacaacctt cgattacggg gaggttgagt taagctccag
      181 tgaaattacc gagaccgata ctcttgacat atccgtaagt gttacgaata ccgggaagta
      241 ctatggtcga gagatggtgc agctctacat tcatgataat gtagcctcgg ttgccccacc
      301 cgtaaagctt cttaagcaat ttcaatttgt tgaccttaaa cccggtgaga gtaaaaaaat
      361 ggtattcaag cttaccgtaa acgacttaat gttctaccgg gatgatatgt cctacggatt
      421 tgaaaaggga caattcactg tatatattgg tggtaattcg cgtgatgtaa ggacagcgtt
      481 tttttcttta aattagagtt tgttttaggt ttggtttgga gaataggtgg aagtatattc
      541 cacctaatct tttattagtg aatggagtaa gtggggtgag ctattttact acattttttg
      601 agatggatta gttcgtggtg aatgcagctg ttgattgagt atttcaccat ttatccaggc
      661 actctacgaa gttaagctcc cacttgtcaa ggtcaagggc aaccaggtag ccggtaccgg
      721 ggaaaagcat ccccctgtac acacaaccgg catccaaccc aaaaattcgc ctgttgggct
      781 gcttgagttt gtcggctatc acatctagcg cttcgggggt atgcccatgt attatcctgt
      841 aatcctgcat aaaatcgttt ggggcatcgt caagcctgct ccagagcatg gcttctgtat
      901 cctccagggg attggctgag gtagtgttca gcccgccatg tacaagaatg tacttatcgt
      961 caacgatgaa gtattgcttt agcgaggtga aaaagtccaa atgtgcttta ggaagaaagc
     1021 ccaaatgacc atcatcatca aaaccaaaca ttttaacgta gcttttaatg gtggcttgac
     1081 ccgagttggc ctgccaaagg catagctccg actctctctt taccgagtca agcatcatct
     1141 gctcgtggtt tccaataagg cagctgatgg tgtagcccga tctcattaac ccaatgatgt
     1201 actccactac tgccgagcta tcggggcccc tatcaatgta atcgcccagg aagaaaagcc
     1261 tgtcctgtag cgataccttt agctttcctt caataagcat cctaagggtt tgtatgcaac
     1321 cgtgaatatc ggaaatgatg taggtggcca tggaagtaat attctattca aatgtacacg
     1381 acattttttt aacatgctga tgaattttta aattattgaa tgattttttg gtctgaagca
     1441 gataaggctt atttttaact agtttttacc agcttcaacc atgtttttag agtgatattt
     1501 tcatttttag gatttagaaa actttttagt cgggtgctat ttagaatcgt tttaaattgt
     1561 agcgctgtaa aacaaaaatg gataatggta tataaagaag tttatttttg tagctgttat
     1621 tatttattta gaaatgcctt caggaaataa tgtagttgag catctgggac gtgttgatgc
     1681 cataacaaat aacgacatcc gggttgttat aaccagccag tctgcatgcg agtcgtgcca
     1741 tgcccggggc gcatgtctgg cttcggaaag caaggaaaag tttattgtag ttactatacc
     1801 caaccataat tttattgtag gcgaacctgt taaggttcta cttaaggaat ccttaggatt
     1861 tcgtgccctt tttttgggtt atgttcttcc attgcttgtt gttctcacaa cccttttcac
     1921 gctgagcgca gccggtatgt ccgaaaagga cgccggttta ctttcgctgg cagtcttggc
     1981 tccctactac cttgggcttt atatttttag aaagaagatt gcaaggaagt ttgaatttgg
     2041 aattgaaaaa atttcataat aaaaacatcg tatgagttca gttcttacta cgtctatttt
     2101 aatgctcacg attttggggg ttgttttagc cgtaatcctt tatgttgttg cgcaaaagtt
     2161 taaggtagaa gaagatccca ggattgatga ggttgaagcc ctgctgccag gtgccaactg
     2221 cggcggatgt ggttatcccg ggtgcagggg gttggccgat gcgcttgtta aggcagacga
     2281 tatcagcgcg atttcttgcc ctgttggcgg taacgaaacc atgagaaagg tggctgccat
     2341 acttggtaaa gcagttgagg acaaggatcc gcaaattgcc gtagtgcgtt gttcgggaag
     2401 cccccaaaac aggacgcgag ttgtagagta taacggaata tcttcttgca aatttgcatc
     2461 cacactttac agcggcgata cgggttgcca attcggatgc ttaggtcttg gcgactgtgt
     2521 tgcggtttgc aagtttgatg ccatctatat tgaccccgtt tcccttcttc cggttataga
     2581 cgaggagaag tgcactgctt gtggggcatg cgtaaaggca tgtccaaaat tacttataga
     2641 gctgcggaaa aaggggccaa agggcaagcg aattttcgtg tcgtgcatga ataccgacaa
     2701 gggtggtgtt gcacgtaagt actgcagcgt tgcatgcatt gggtgtttca agtgcgtgaa
     2761 ggtttgccct tacgaggcca tcaccctgga gaataacctg gcctacattg atgccaacaa
     2821 gtgcaagctt tgcaggaagt gcgtggaaga atgcccaacc gattccattt tggaggtaaa
     2881 cttccctcca cgtaagccga aggttgagga acaagcccag aacgcagtaa ccagctagca
     2941 taagccatat caaacttaat tttaaaaaaa attgctaaac gtgttgaaaa catttaaaaa
     3001 ggggggagtt catccacctg aaaataagat ttcagcagct caaccggttg aggttttgcc
     3061 tcttccggct aaagtcatca ttcctctgtc gcagcatata ggtgccccgg cagaggcgat
     3121 ggtagccaaa ggcgataggg taaaaaccgg acaactcata gccaaaagcg ctggctttgt
     3181 gtcggccaat gtccattcaa ctgtttcagg aatagtagag tcccttgaac ccgtgatgga
     3241 tagttcaggc tataaacggc ctgccataac catcaacgtt gagggagatg attgggagcc
     3301 ttcagttgac cgttctccca acttaaagaa ggaaataacc ctttcggctc aggatattat
     3361 aaaaaaaata gcggaggccg gtattgtggg ccttggcggt gcaacatttc caacccatgt
     3421 aaagctttcc gttcccgcag gcaagaaggc tgaaatgctg gtgattaatg gcgttgagtg
     3481 tgaacccttt cttacctctg atcataggct gatgctcgag aaaggccacg agcttatgat
     3541 tggcattcag attttgatga aagctctgaa tgtgcctaag gctatggttg gtattgaggc
     3601 gaataagccc gatgctgtat cccatctcca atcactggcc aagtcataca gcggaataga
     3661 ggttcaacct ctaaaggtga agtaccctca gggtggcgaa aaacagctta ttaaggcgct
     3721 taccggccgt gaggttcctt cgggtggatt gccaattgat ataggtgcag ttgttcataa
     3781 cgtgggcaca gcccttgcag tttacgaggc ggtgcagaaa aataagccgc tggttgagcg
     3841 cattgttacc gttacgggta aaaatcttgc ccatccatca aactttttgg taagaattgg
     3901 aacgccagtt tcggcactga tcgccgctgc aggtggccta ccagatgata caggtaaggt
     3961 ggtgagcggt ggccccatga tgggaaaggc agttagctcc attgatgtgc ccgttacgaa
     4021 aggaacttcg ggaatattgg taattcctgc tgccgaatca aaacgtaaac ccgaaggtgt
     4081 ttgcattagg tgtgccaagt gcgtttctgt ttgccctatg gggttggagc cctacttgct
     4141 ttaccggctg gggcagcgaa tgcagtatag ccaaatggag gtagagcgtg tgctcgattg
     4201 catggagtgc ggttcttgca gctttgagtg tccggctaac ttgtttttgc tcgactttat
     4261 caggctaggc aaggttgagg taggcaaaat tgttagggag cggaaaaatt aacatttaaa
     4321 gtatatactg acagtaacct tttaaacgaa tggataatcg ttttgtagtt tcaccatcgc
     4381 cccatgtgca tagcggcaaa tcgaccagcc agctgatgct gaatgtggtt ttagcgctga
     4441 tgccggcgtt tgccgtttcc atttactact ttggcgtggg agccataatt gttactctaa
     4501 tatcggttct ttcgtgcgtg ctattcgagt acctgattga gaagtttatt ttcaaggtta
     4561 agccgacaat atccgattgc tctgcggttg taacgggttt gctgctggca tttaacgttc
     4621 cgagcaatct gccgcggtac attatagtgc tgggaagcct ctttgcaatt ggcgttgcca
     4681 agatgagctt cggagggctg ggcaataata ttttcaaccc ggccatagcc gcacgaattt
     4741 tcctgctgat atcctttccg gtgcaaatga ccagctggcc gctaccggtt acctctcgtt
     4801 tacagtatat tgatgctatt accggtccaa cccctttggc tattattaag gagggggtta
     4861 agaatggcca aaccatttcg caaatggccg acagcattcc ttcccacatg cagcttttca
     4921 tgggcttgat gggtggctcg ttgggggaga ttgctgcttt cgccttgctt attgggtttg
     4981 catggcttct cattacccgc gtaattacct ggcatattcc ggtatcaatt tttactacca
     5041 ttttcgtatt cacaggaatt ttatggctta tcaaccccgg tatctatgcc gacccggttt
     5101 tccacctcct taccggtggt gtgatgctgg gggctatatt catggccacc gactatgtta
     5161 cctcacccat gcatcccaaa ggcatgtgga tatacggaat aggtattggc gcgcttactg
     5221 tgataataag ggtttggggc tcgtaccctg agggagtttc gtttgccatt ctgattatga
     5281 acgctttcgt accccttatc aaccgattcg taaagccaaa acgttttggt gcagcagcaa
     5341 taagcggtaa atagcatgta aaacttaaac atcaaaatat gtccggtgta gaatcatcat
     5401 ttaagaatat gaccatttcc ctgctggcga ttacccttgt tgcatctgcc ggattggggc
     5461 tggtttataa atcaaccaag gatc
//
LOCUS       DAOOQX010000038         5478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_17954_length_5478_cov_3.221280, whole genome shotgun sequence.
ACCESSION   DAOOQX010000038 DAOOQX010000000
VERSION     DAOOQX010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17954_length_5478_cov_3.221280"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..488
                     /locus_tag="PLX53_01190"
     CDS             285..488
                     /locus_tag="PLX53_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOY71726.1"
                     /translation="MHAVVVNQEKCPRNHPCPVVRICPTGAISQKSWNSAPEISADDC
                     LACGKCVTSCGYGAFRFDNAGAK"
     gene            526..1047
                     /locus_tag="PLX53_01195"
     CDS             526..1047
                     /locus_tag="PLX53_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOY71727.1"
                     /translation="MKKLSGYFLSVLLVAAMLSACDGNNPAKGQQESAPAAPTKTGSK
                     ITGTYAVDITREEFLTKILDYTQEPTNVKYLGNKPAIVDFWASWCGPCRIASPILEEL
                     AKEYHGKILVYKVDTQKEQQISAELGIQSIPTFIFFPVDGKPFATSGIASTPEDTRKM
                     FVEIIEKELLGNR"
     gene            complement(1079..1525)
                     /locus_tag="PLX53_01200"
     CDS             complement(1079..1525)
                     /locus_tag="PLX53_01200"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HOY71728.1"
                     /translation="MRQTIPFRIVELEPDSYHMVVEASMSGKSVHLIVDTGASRSLID
                     ISSDSGLRVEGVDETLAVGFNTDKISIQLAIFPYIGVGGFTFSDFVAAVTDLDALRSL
                     YSKVAGMDIAGLLGCDFLVRYVSSINFRTKRIYLKQLTAEVKSSQE"
     gene            2236..3159
                     /locus_tag="PLX53_01205"
     CDS             2236..3159
                     /locus_tag="PLX53_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOY71729.1"
                     /translation="MKNSRKDLVRIGVFYDGNYFLHVSNYYNYFHPRKKRLNIAGLHE
                     FIRNLVASEEEADPRNCQVIEAHFFRGRISAQEASQRGNQLYNDRVFDDILMSEGVVT
                     HYLPIRPRGGRREDKGIDVWLALEAFEQTFYKRFNVLVLITSDGDYVPLIRKVNSLGT
                     KVMVLSWDFDYTDDQGREVTTRTSQELIREVTYPVAMHELINSGLKSKDRLTENLFTV
                     QDFGQAKPVEKPVSPEEIKQARVLSLNSGYGFIQYPPNNLFFYYLDVVDGDFDELIEG
                     DMVEFTISKNDRGQDVAKNVRKIPEYNANKW"
     gene            complement(3282..>5478)
                     /locus_tag="PLX53_01210"
     CDS             complement(3282..>5478)
                     /locus_tag="PLX53_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOY71730.1"
                     /translation="TSLETIRALLSFFKKFTDRYPAIWELAKNVAYYPSVVERINALL
                     DKFGRIKDNASPQLAQIRSAITSKQSSVSKLVQTILRSARDQGIIEQDASPSVREGRV
                     VIPVAAANKRKLKGIVHDESSTGKTVFIEPVEVVELNNEIRELEYDERREVVRILVEF
                     SDSIRPYLPELLGAYDFMGMVDFIRAKALFAIEYQGAKPIIKETTGTYLRQAKHPLLQ
                     LSLKREGKEIVPLDIELKSEERILLISGPNAGGKSVCLKTVGLLQYMLQCGFLPCVLE
                     NSEMGLYRKIFIDIGDEQSLENDLSTYSSHLLNMKYFLKNATPETLILIDEFGSGTEP
                     SAGGAIAEAILQGFANTGTYGVITTHYSNLKHFAASTRGIVNGAMLFDSSRIEPLFKL
                     EIGKPGSSYAFEIARKIGLPDSIINEATAKVGDDYVTFEKHLREISRDKRYWEKKREN
                     IRLASKKAEAEEAQLTRELEELASQRKEILAAAKKNAQELLAETNRKIENTIRIIKES
                     NAQKEETRKARQELEQFKQQVDEAAHNLNDEVERKMERIKQKQEKRALHRKAKEPAAS
                     SRTEPVASPPLTIGDRVKIIGSDLLGEVVGLSGQNVSVAVGNLISMVNLERVQRISTA
                     EYRNANKAVTQPQVGTGFDTYKRRLNFKSDIDIRGARADEALETVQNLVDDALMLGFG
                     RVRILHGKGNGILRSVIRDYLKSTPGVKAFSDEQEEFGGAGITVVDLDV"
BASE COUNT         1437 a         1230 c         1258 g         1553 t
ORIGIN      
        1 ggtggttagg atggattgcc gttggattgt ttcgggtact ttcaatgtaa ccgttaataa
       61 caatagcatg tctctcgact atggaaatgg agtttgcgat aacctggcta ttgcaaccat
      121 taacggaagg gcctacgagg ttaggctgag aaagtaatcc aaacaatgtg taaacctaaa
      181 gggaggcagt agcctccttt ttttgttaaa atatatgaat aaatatgtaa atatatacat
      241 acatgtatta aatttgcaat gttaaccatt aaaattcaaa caaaatgcat gctgtagttg
      301 ttaatcagga gaagtgccca agaaatcacc cctgcccggt agtaaggata tgcccaacgg
      361 gggcaatcag ccagaagagt tggaactcgg cacctgaaat tagcgctgat gattgcctgg
      421 cctgtggcaa gtgcgtaacg agttgtgggt atggtgcctt caggtttgat aatgcgggtg
      481 cgaaatagct actttacagt tcaactacat aaattgatta ctcaaatgaa aaaactatca
      541 ggatacttct tgtcagttct actcgttgct gccatgctga gcgcatgcga cgggaacaac
      601 cctgcaaagg gtcagcagga gtcagctcca gcagcgccta ccaaaacggg ttcaaaaatt
      661 accggtacct acgcggttga tattaccagg gaggaattcc tgactaaaat tctggattat
      721 actcaggagc ctacaaatgt caaatatctt ggcaataagc ctgccattgt tgatttttgg
      781 gctagctggt gcgggccatg ccgcatagca tcccccatac tcgaggagct cgcaaaggag
      841 tatcacggta aaattctggt ttacaaggtt gatacccaga aggagcagca aatttcagcc
      901 gagctgggca tccagagcat ccccacattc attttcttcc cagtggacgg caagcccttt
      961 gctacttctg gaattgccag cacccccgag gatacccgca aaatgttcgt cgaaattatt
     1021 gaaaaggaac tcctaggaaa ccgctagccc cataagcata gcttaaattg ggaattactt
     1081 actcttggct actttttacc tcagcagtca gctgcttgag gtaaattcgc tttgttcgga
     1141 agttaatgga cgagacgtaa cgaaccaaaa agtcgcaccc cagcaaacct gcaatatcca
     1201 ttcctgcaac ttttgagtag aggcttcgca gggcgtcaag gtcggtaacc gcagccacaa
     1261 aatcactaaa agtaaagcct cctactccga tgtatggaaa aattgcaagt tggatgctga
     1321 ttttatccgt attgaaacca acagccagcg tttcatcaac tccctcaacc cgcaagccgc
     1381 tatcagagga tatgtctata agcgagcgtg atgctccggt gtcaacgatg aggtgcaccg
     1441 atttaccact catggaggct tccaccacca tatgatagct gtctggctcc agctccacta
     1501 tacgaaaggg gattgtttgg cgcattcaat agcttgtatg aagaataact cctggttaac
     1561 ttctctattc tgctgcaaag ctagcaatct ttttatcatt tggcttttct cgtaatggtc
     1621 tgcttttgct ccccgagatt ttgaaatcaa aaatttagct aatttctatc aattttgaac
     1681 caattactcc ctatgatgtt cttgtaatta cgcctgaaaa ccgtattttt gcctatccaa
     1741 aagatatgtt taaaccacat aaattttaat ataaacattc atattggttt tacgatttaa
     1801 caatcaaata gacaggcagg ttgataggtc attttgcatt taaaaaataa gttaagttga
     1861 aatactcaca tggcgcttta tgattttata gcttgagccg tatgcaattg ctggtaagct
     1921 tgtctaggtt gaaaaaataa actctttagc gatataccag agcatgcaat attaaaagaa
     1981 tgagctaatc gcttagtatc aagagaaaaa ttgcgcaaag agttttaaat cttcatgccg
     2041 accggtatct gccttgatgc atattgtaaa gctaagcgat aaattagcgt tggaagtata
     2101 ggactttcta gtggagcaac catagtctct gacgttgtgc agagtaaatg ggagttgtgc
     2161 caacagggcg ggagtattac agagtaatca aaaccaaata gctactaatc aggcatataa
     2221 ctaaatttta ttcttatgaa aaattcaaga aaagaccttg tgcgaatagg agttttttac
     2281 gatggaaact actttttgca tgtaagcaac tactacaact actttcatcc aagaaaaaag
     2341 aggctgaaca ttgccgggtt gcacgagttt ataaggaatt tggtggcttc ggaggaggaa
     2401 gcagatcctc ggaactgcca ggtgatagag gcccattttt ttaggggcag gataagcgcc
     2461 caggaggcaa gccaacgggg taaccagctt tataacgacc gggttttcga cgatattttg
     2521 atgtccgagg gcgttgtaac ccactacctg ccaattaggc cacggggtgg acgccgtgag
     2581 gataagggta tagatgtttg gttggcactc gaggcctttg agcaaacctt ctacaagcgt
     2641 tttaacgttt tggtgcttat cacctccgat ggcgattatg ttccattaat tcgtaaggta
     2701 aactctttgg gaactaaggt gatggtgctc tcttgggatt ttgactacac cgatgaccag
     2761 ggtcgcgagg ttaccacccg gacctcgcag gagcttattc gcgaggttac ctacccggtg
     2821 gccatgcacg agctgataaa ctcggggttg aaaagtaagg acaggttaac cgaaaacctc
     2881 ttcactgttc aggactttgg tcaagctaaa ccagtggaaa agcccgttag ccccgaagag
     2941 atcaaacaag ccagagtcct tagcctgaat agcggctacg ggtttatcca gtatcctccc
     3001 aacaaccttt tcttctacta ccttgatgtg gtggacggcg atttcgacga gttaattgag
     3061 ggggacatgg tggagttcac catctctaag aacgacaggg gccaggatgt ggctaagaat
     3121 gttcgcaaaa ttcctgagta caacgccaat aaatggtagt tggtggcaaa ttaaaatggc
     3181 tagcgtaacg ggttgggcga gaattcgctc aaccctttta aactgaaatt gttttcctga
     3241 gaattttatg gagggaaaaa actaccgtat gtatgccgga cctaaacatc caaatcaaca
     3301 acggtaattc ctgctccgcc aaactcctcc tgttcatcgg agaaagcctt aacacccggt
     3361 gtagatttaa ggtaatcgcg aatcacactg cggagaatgc cgttaccttt cccatgaagt
     3421 attcttaccc ttccgaagcc aagcattaag gcatcgtcaa ctagattttg tactgtttca
     3481 agagcctcat cagctcgtgc tccacggatg tcaatatcgg atttgaagtt gaggcggcgc
     3541 ttgtaggtat cgaaacccgt tccaacctga ggctgggtta cagccttgtt ggcattccta
     3601 tactccgctg tggatatcct ttgcaccctt tctaggttta ccatgctgat aaggttacca
     3661 acagcaaccg atacattttg acccgacagg cctacaactt ctccaagtag gtcgcttccg
     3721 ataattttta ccctatcgcc aattgtcaat ggaggggatg ctacaggttc tgttctgctg
     3781 cttgctgccg gttcttttgc ttttctatgc aacgcccttt tttcttgttt ttgtttaatg
     3841 cgctccatct ttcgctctac ctcgtcgtta aggttatggg cagcttcgtc aacctgctgc
     3901 ttaaactgct ctagctcctg acgggctttg cgggtttcct ccttttgtgc attcgattct
     3961 ttaataatcc ttatggtgtt ttcaattttc ctattggtct cagctagcag ctcctgtgca
     4021 tttttttttg cagcagccag aatctcctta cgctggctgg ctagctcttc gagttccctg
     4081 gtcagctgtg cttcttctgc ttccgctttt ttgctggcca gacgaatgtt ttcccttttt
     4141 ttctcccagt accgcttatc gcgtgagatt tcacgtaaat gtttttcaaa ggtaacatag
     4201 tcatcgccta cctttgccgt agcctcgttt ataatgctgt ccggtaggcc aatctttcgt
     4261 gcaatttcaa atgcgtagct gctaccaggt ttgccaattt ccagcttaaa taggggttct
     4321 atacgggagc tgtcaaaaag catggcgcca tttacaattc cacgagtgct ggcggcaaaa
     4381 tgcttcaggt tggagtagtg ggtggtaatt acaccgtagg ttccggtatt ggcaaagccc
     4441 tgaaggattg cctcggctat tgctcctcct gccgacggct cggtgcccga cccaaactca
     4501 tcgatgagaa tgagtgtttc aggtgtggca tttttcagga aatacttcat gtttagcagg
     4561 tgggagctgt aggtgctgag gtcgttctcc agcgattgct catcgccaat atctatgaag
     4621 attttgcggt ataagcccat ttctgaattt tccaatacgc agggcagaaa accgcactgc
     4681 agcatgtact ggagtaggcc aacggttttg aggcatactg atttaccgcc cgcatttggg
     4741 ccggaaatca gaagtattct ctcttcggat ttaagttcaa tatccagcgg gacgatttcc
     4801 tttccttcgc gtttcaagct cagctgcagt agtgggtgct tggcttgtcg gaggtaggtg
     4861 ccggtggtct ccttgattat gggctttgca ccctgatact caatggcaaa tagggcttta
     4921 gctctaataa aatcaaccat tcccataaaa tcgtatgctc caagcagctc gggaagatag
     4981 gggcgaatac tatcggagaa ctcaaccagt attcgaacaa cctcgcggcg ttcatcgtac
     5041 tccagctccc ttatttcgtt gttcagctct accacctcaa ccggttcaat aaaaaccgtt
     5101 ttgcctgttg atgattcgtc gtgaactatt cccttaagct tacgcttatt agccgctgca
     5161 accggaataa ccaccctgcc ctccctaacc gagggtgagg cgtcctgttc aataatgccc
     5221 tggtcgcgag ctgatctcag aatggtttgc accagctttg aaacggatga ttgttttgat
     5281 gttatagccg agcgaatttg ggccagctgt ggagaagcgt tgtctttaat tcttccaaac
     5341 ttgtcgagta gggcgtttat gcgctccacc acggaggggt aataggctac gtttttggcc
     5401 aactcccaga tggcgggata cctgtcggta aattttttaa agaagctaag aagcgcccga
     5461 atggtttcca gcgaggtg
//
LOCUS       DAOOQX010000039         5461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_18010_length_5461_cov_3.050869, whole genome shotgun sequence.
ACCESSION   DAOOQX010000039 DAOOQX010000000
VERSION     DAOOQX010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18010_length_5461_cov_3.050869"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PLX53_01215"
     CDS             complement(<1..684)
                     /locus_tag="PLX53_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HOY71731.1"
                     /translation="MAGIYVHIPFCRRKCLYCDFYSVGERFITGDFDKLINHELLLRG
                     NFLNDKRISTVYLGGGTPSLLPIESVGRILEGIDTLSSLNGDAEITIEVNPDDVSLEL
                     FRSYKRLGVNRISIGVQSFNQAELSFLGRRHSVESSSVAVHNAQDAGFENISIDLIYG
                     IPGSTLQTWEHSLSVAENLNPQHLSCYHLTYEGNTPLTRRLRKGLFTALDEELSLGQF
                     ELLRSFAQSK"
     gene            814..1257
                     /gene="rpiB"
                     /locus_tag="PLX53_01220"
     CDS             814..1257
                     /gene="rpiB"
                     /locus_tag="PLX53_01220"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOY71732.1"
                     /translation="MEKEKLAIASDHAGYHLKEDLKQFLGELGYHVWDFGTHSEDSVD
                     YPDFAHPLAQAVENGEYRFGFLLCGSGNGVSIAANKHQGIRAALCWIPEIASLARMHN
                     DANVCSIPARFVSAQEAKLIAKAFLDARFEGGRHSRRVEKIPLSK"
     gene            1281..2258
                     /locus_tag="PLX53_01225"
     CDS             1281..2258
                     /locus_tag="PLX53_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOY71733.1"
                     /translation="MDQNKPISSFIEKHYLHFNAASLVDAAKAYKKHLADGKKMMITL
                     GGAMSTAELGISLAEMIRQDKVQIITCTGANLEEDLMNLVAHNHYKRVPDYRDFTPQQ
                     EWDLLQSGLNRVTDTCIPEEEAFRRLQSHLFDIWKSALDKGERFFPYEYMYKLIRSGV
                     LKQYYEIDPKNSWMLAAAEKNLPIIVPGWEDSTMGNIFAAYCIKNELKPSIMKTGIEY
                     MMFLADWYTKNAGKEGIGFFQIGGGISGDFPICVVPMLTQDLEREDTPFWSYFCQISD
                     STTSYGSYSGAVPNEKITWGKLGINTPKFIIESDATIVAPLIFAYVLGW"
     gene            2445..3671
                     /locus_tag="PLX53_01230"
     CDS             2445..3671
                     /locus_tag="PLX53_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOY71734.1"
                     /translation="MNIEEYLAGFSLSGSAGIKTQVLDLPVRGSTYPVFINEYWTSKQ
                     RQASSIHEISYRACFKPQLPHFFIERLTAEGETVYDPFLGRGTTIIEAALMGRNVAGN
                     DVNPLSLMLTRPRLFIPNLADVQQRLSSIPVNSSLRASSDLSMFFHPQTEAEIVSLRN
                     YLDTRKQQEKEDEVDQWIRMVATNRLTGHSVNFFSGYTLPPNQAISAEKQKMLNIKHG
                     KTPGYKNVKSIILKKSQDLLRNISEEETSRLRKAGEKAILLNTDARHANTLPDNSVSL
                     TITSPPFLNVVQYAADNWLRCWFNNINIQDVASRITMSKTIEEWENVMREVFVMLYSK
                     TKHKGYVAFEVGEVRNGKVKLENHVVPLGLEAGFDCVGILINQQKFTKTSNIWGVSNN
                     SRGTNTNRIVLFRKSK"
     gene            3943..>5461
                     /locus_tag="PLX53_01235"
     CDS             3943..>5461
                     /locus_tag="PLX53_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71735.1"
                     /translation="MNKILSKALPHVIAVLVFMAISLAYFYPQIQGKRLYQHDIAQWR
                     GSAKEIMDFKEKTGKETLWTNSMFGGMPAYLISANYSGNLMLKVNKAMNFLQRPASFI
                     FIAMLGFYILLLVFGLNPWLSLVGALGYGLSTYFFIIIGAGHNSKIQAIGYVAPMIAG
                     LVMACKGRFWGGMAIFGFFFGLNLASGHPQITYYSAFIMLALLVAFFINAQKTKTLKT
                     IYKAIGFLAIAGILAVGSNFSNLWFTYDYGKDSIRGKSELTDNQHNKTSGLDKDYVTQ
                     WSYGVAETFNMLIPNLMGGASSMDVGENSETFKFLRQNGVPFSQAQTIVKQLPTYWGP
                     QPSTSGPVYVGAIIFFLFILGMFLLRGPEKWALFAVTLLAILLAWGHHFNFLTNFFLN
                     YFPAYNKFRTVSMILVVAELTMPLIGFLGLKEIYEGKIAKPDFLRAFKWATGITAGIC
                     LFFLIFGRGIFNFSGAVDQQLGWPKELIDVLKRDRQHMLTNDSLRSLFFVLAGAGI"
BASE COUNT         1533 a         1307 c         1224 g         1397 t
ORIGIN      
        1 ctttgattgg gcaaaactac gaaggagttc gaactgccca aggctcaact cctcatcaag
       61 ggcggtaaac aatcccttcc ttaacctacg ggtaagcggg gtgttccctt catacgtaag
      121 gtggtagcac gaaaggtgtt ggggattcaa attttctgcc acacttagtg aatgctccca
      181 ggtttgaagc gttgagccgg gaatgccgta tatcaaatct atgctgatgt tttcaaaacc
      241 tgcatcctga gcattatgca cagcaaccga ggaggattca acgctatgcc gtcgccccag
      301 aaagctaagt tctgcctggt tgaacgactg cacgcctata cttatcctgt ttacacccag
      361 cctcttatag ctccgaaaaa gctccaacga aacgtcatct gggtttacct caatggtgat
      421 ttcagcatca ccattcaggc tgctaagagt atcaatgcct tcaagtatcc tcccgacact
      481 ttcaatggga agcagggagg gagttccccc gccaaggtaa acagtgctga tgcgcttgtc
      541 gtttaagaag tttcctcgta gcagcagttc atggttgata agcttgtcaa aatcaccggt
      601 aataaacctt tcccccaccg agtaaaaatc acagtagagg cacttgcgcc tgcagaaggg
      661 aatatgcacg tatattccag ccaccttgtc accaaatgat tacgacacaa aaacagatat
      721 aaaagccaaa aagccattat ctttgcaaag ttaacgttat acgaacacaa accatttcat
      781 ctattggcag aaaaggtaat tatacccggc aacatggaaa aagaaaagct agccatcgcc
      841 tccgaccatg caggttacca cctgaaagag gatttaaagc agtttcttgg ggaattggga
      901 tatcatgttt gggattttgg aacgcacagc gaggatagcg ttgactatcc cgattttgcc
      961 catccgctgg cacaagcggt cgagaatggc gaataccgtt ttggattctt gctttgcgga
     1021 agcgggaatg gcgtatcgat agctgccaac aagcaccagg gaattagggc tgcactttgc
     1081 tggatccctg aaattgccag ccttgcccgt atgcataacg atgccaacgt ttgctcaatt
     1141 ccggcccgat ttgtttcggc tcaggaagct aagctaattg caaaagcctt tctggatgcc
     1201 cggtttgaag gtggccggca ttcccgtaga gttgaaaaaa ttccactatc caaataacaa
     1261 cccaacaata ttctaagaaa atggatcaga acaaaccaat ctcctcgttt atcgagaagc
     1321 attacctgca tttcaacgct gcttcacttg ttgatgcagc taaggcctac aaaaaacacc
     1381 ttgccgatgg caagaagatg atgatcaccc ttggcggcgc catgagcact gccgagcttg
     1441 gtataagcct ggccgaaatg ataaggcagg ataaggttca gattataacc tgtaccgggg
     1501 caaaccttga ggaagacttg atgaacctgg tggcccacaa ccactacaaa cgagttcccg
     1561 attaccgcga ttttactcca caacaggaat gggatttgct tcaaagcggc cttaacaggg
     1621 ttacagatac ctgtatcccc gaggaggagg cattcaggag gctgcaatca cacctcttcg
     1681 acatttggaa gagcgccctc gacaaggggg aacgtttttt cccctatgag tacatgtaca
     1741 agctaattcg tagcggtgtg ctgaagcagt actatgagat tgatcccaaa aactcatgga
     1801 tgcttgctgc tgccgaaaaa aacctgccaa ttattgttcc aggctgggag gactctacca
     1861 tgggcaatat ttttgccgcc tactgtatta aaaacgagct taaacccagc atcatgaaaa
     1921 ctggtataga gtacatgatg ttcctggccg attggtacac caaaaatgcc gggaaagagg
     1981 gcattggctt cttccaaatt ggcggaggaa tttcaggtga tttcccaatt tgcgtggttc
     2041 ccatgcttac acaggacctg gaacgagagg atacaccttt ctggagctac ttctgccaga
     2101 ttagcgattc caccaccagc tacgggtcat actccggagc agttccaaat gaaaaaatca
     2161 cctggggaaa gctgggcatc aacaccccaa aattcatcat cgaatcggac gctactattg
     2221 ttgcccccct aatatttgcc tacgtgctgg gttggtagaa ggtgaactca gctccattgc
     2281 gcagcaatac aatagatgtt ggcttggccg gggagaactc tccggccttg cttttgaaca
     2341 taaccctggc aagccaaaaa gtcaaatatt cccccgatgc acccccatgc tttttcttgt
     2401 ggtcattcct tcctaaattt gtgcagttgt aaagtagcta aaccttgaac atagaagaat
     2461 atctggcagg attctccctt tccggcagtg ccggcataaa aacccaggtg ttagacttac
     2521 cggttagggg ctcaacatac cctgtattta tcaacgagta ttggacatca aaacagcggc
     2581 aggcaagctc catccacgaa atctcctacc gggcttgttt taaaccccaa cttccgcact
     2641 ttttcattga acgcctgacc gccgagggcg aaactgttta cgatccattt ctggggcggg
     2701 gaacaaccat tattgaagct gctctcatgg gccgtaacgt tgcaggcaac gatgtaaacc
     2761 cgttgagcct tatgctcacc cgacccaggt tgtttatccc aaaccttgcc gatgtgcagc
     2821 aacgactttc cagcataccc gttaattctt ccttaagagc aagttccgac ttaagtatgt
     2881 tttttcaccc ccagaccgaa gcagaaatag tgagcttgcg taattacctg gacacaagaa
     2941 agcaacaaga aaaggaggat gaggtggacc agtggatacg aatggtggca accaacaggc
     3001 ttacgggtca ttcggtcaac ttcttttcgg gctacacgct accgcccaac caggccataa
     3061 gtgccgaaaa acaaaaaatg ctgaacatta aacatggtaa aactcctggc tacaagaacg
     3121 ttaagtcaat cattctgaaa aaatcgcagg atttactgcg gaatatatcc gaggaagaaa
     3181 ccagccgcct gcgcaaggcg ggggaaaagg ccattctcct gaacaccgat gcacgccatg
     3241 ccaacaccct gcccgataac tcggttagcc ttaccataac atccccgcct ttccttaacg
     3301 tggtgcagta tgcagccgac aactggcttc gatgctggtt taacaacatc aatattcagg
     3361 atgttgcatc ccgaattacc atgtctaaaa ccattgagga gtgggaaaat gtgatgcgtg
     3421 aggtattcgt gatgctttac tccaaaacta aacacaaggg ctacgtggca tttgaggttg
     3481 gtgaagttag aaatggtaag gtaaagctgg agaaccatgt ggtaccctta ggcctcgaag
     3541 ccggttttga ctgcgtggga atactaatca accaacagaa atttactaaa acatcaaata
     3601 tttggggggt tagcaacaac tcaaggggca ctaacaccaa cagaattgtc cttttcagga
     3661 aatccaaata gcccacattg aaaaaatttt tagtaaagca tcatacctaa aagaactact
     3721 gttcagccat ttcaaatatg attttaaccc atcaccgtaa tttttgcgta tatttgaacg
     3781 ctaaactgtt gagctaccca tgggtaaatt ttgaactgct tcaaaattca ttttaattca
     3841 tagggaatat agctcggtat ggtaagcaag tagaaacata agttttctag gttcatttac
     3901 ttttacaacc agctgtagtg tcagtaacat aaacttaagc agatgaataa gatcctgagc
     3961 aaagccctac cccatgttat tgccgtgcta gtgtttatgg caatttcact tgcctatttt
     4021 tatcctcaaa ttcagggcaa aaggctctac cagcacgata ttgcccagtg gaggggatcg
     4081 gctaaggaaa ttatggactt caaggaaaaa acggggaagg aaaccctatg gaccaacagc
     4141 atgtttggag gaatgcccgc ctacctgatt tcagccaact attcaggcaa cctgatgctg
     4201 aaggttaaca aggcaatgaa ttttctacaa cgtcctgcca gcttcatttt tattgctatg
     4261 cttgggttct acatcctgct gcttgttttc ggattaaatc cttggctgag tttggttggt
     4321 gcgctaggct acggcctatc aacatatttt ttcatcataa ttggagcagg gcataactcc
     4381 aaaattcagg caattggcta tgttgcaccc atgattgcag gactggttat ggcctgtaag
     4441 ggaaggttct ggggcggaat ggccatattt ggctttttct ttgggcttaa cctggcaagt
     4501 gggcatccac aaattaccta ctacagcgca tttatcatgc tggccctgct tgttgcgttc
     4561 ttcataaatg cccaaaagac caaaaccctg aaaactatct ataaggccat cggatttctt
     4621 gcaattgcag gaattcttgc tgtgggatca aactttagca acctatggtt tacctacgat
     4681 tacggcaaag actccataag ggggaaatct gagctaaccg acaaccagca caacaaaacc
     4741 tcgggactcg acaaggatta cgttacccag tggagctacg gagttgcgga aaccttcaac
     4801 atgctaatcc ccaaccttat gggaggcgcc agctccatgg atgttggcga aaactccgaa
     4861 acattcaagt tcctgcgtca aaatggggtt ccctttagcc aggcgcaaac aattgtgaag
     4921 cagctgccaa cctactgggg gccacaaccc tcaacctcgg gacctgttta cgtaggcgcc
     4981 atcatttttt tcctcttcat actaggcatg tttcttctta gaggccccga gaagtgggcg
     5041 ttgtttgcag ttaccctgct ggcaattcta ctagcctggg ggcaccactt caacttcctt
     5101 acaaattttt ttctcaacta ctttccggcc tacaacaaat tccggacagt ctcgatgatt
     5161 ctggtggttg ccgagctaac catgccccta ataggttttc tgggcttaaa ggagatttat
     5221 gagggaaaaa ttgctaaacc agactttctc agggcattta aatgggcaac tggtattacc
     5281 gcaggaattt gcttgttttt cttgatattt ggaaggggca tttttaactt tagcggagct
     5341 gttgatcagc agcttggctg gccaaaggaa cttattgatg tcctaaaaag agaccggcag
     5401 cacatgctga ctaacgactc cctccgttcg ctattttttg ttcttgccgg agcgggcatt
     5461 g
//
LOCUS       DAOOQX010000040         5442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_18087_length_5442_cov_3.105625, whole genome shotgun sequence.
ACCESSION   DAOOQX010000040 DAOOQX010000000
VERSION     DAOOQX010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18087_length_5442_cov_3.105625"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PLX53_01240"
     CDS             complement(<1..720)
                     /locus_tag="PLX53_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOY71736.1"
                     /translation="MNDKLLKPDYLFEVSWEVCNKVGGIHTVMSTKASMIENELKNNY
                     ITVGPDLIKGIEHNAEFIEDTNLFAAWRSHAIENGMPIRVGRWNIPCKPVAILVNFSQ
                     LISKKDEIFKGFWEKFKLDSITGQWDYIEPALFGYAAGKVIESFTNFNLSLRHRIVAQ
                     FHEWMTGTGLLYLKANVPQVGTVFTTHATVLGRSIAGNNIPLYSNIEKIDPESKAREF
                     GVISKQSLERISAQQADCFTTV"
     gene            complement(1263..2435)
                     /locus_tag="PLX53_01245"
                     /pseudo
     CDS             complement(1263..2435)
                     /locus_tag="PLX53_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
     gene            complement(2532..3824)
                     /locus_tag="PLX53_01250"
     CDS             complement(2532..3824)
                     /locus_tag="PLX53_01250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOY71737.1"
                     /translation="MRVLMFGWEFPPHITGGLGTACFGLTRGLTKQKVDVIFVVPKAY
                     GDEDKSAVRLVAAEDVDVAYSAKNLEEFWRHLKYLEISSNIVPYVDPEEYAKIIEHNE
                     AETVEYESGKVKTRFKFSGKYNPNLMQEVTRYAVVAANLAAREKFDVIHSHDWLTFPA
                     GIAAKMVSGKPLVVHVHATEFDRSGENINQAVFDVEQRGMMVADKVITVSNYTRNIVI
                     TRYGIPEDKVVTVHNAVDFSDEQDMEFERGVSEQIVTFLGRVTFQKGPDYFVEAANKV
                     LKRTHNVRFVMAGSGDMLNRMIRRVAQLGIAHKFHFTGFLKGPDVNRMFHLSDVYVMP
                     SVSEPFGISPLEAMRSSVPVIISKQSGVSEVLKYAIKVDFWDIDALADSIYGLLNYNA
                     LSTFFIRHGQEEVNSLKWDNAASKVIEAYQDVLSRKNS"
     gene            complement(3835..>5442)
                     /locus_tag="PLX53_01255"
     CDS             complement(3835..>5442)
                     /locus_tag="PLX53_01255"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOY71738.1"
                     /translation="GVILKKELLFVHNEEQVMIRYTLLDAHSPTKLRMKPFMAFRNVH
                     SLSKANMWANTKYQECSNGVRYKLYTGFPFLYMQLNRQNEFVASPDWYYNVEYMEEIR
                     RGYEGHEDLFVPGYFETSIRKGESIIFSAATNEQSTPALLRKFNSEQTRRPPKDSFMN
                     CIANTARQFIIQRGKRYDIIAGYPWFSSWSRDSFISLPGLTLGLDDVKTCKALLDSLS
                     TDLKNGLFPNVRTGNSEAFNSADAPLWYFWAIQQYASYTQNYTEVWDDYGKKMKAILK
                     GYREGLPFSIKMQPNGLIWAGEKGYAITWMDAVIQAKPVTPRIGYPVEINALWYNALM
                     FTLELATKNKDLRFVKEWQPIADQVKKSFNELFWIPEKNYLADYVDENGQNWDIRPNQ
                     LFAVSLPYSPIDDEKKIAVLNVVSHELYTPKGLRTLSPNNPNYKGRYEGDQPTRDMAY
                     HQGTAWPWLLGAYVEAQFKLQGKGALGLAQEIVDSLQEDIALHGICSVAEVYDGDPPQ
                     RPNGCISQAWSVAELIRIVKMIEKYSD"
BASE COUNT         1285 a         1222 c         1259 g         1676 t
ORIGIN      
        1 aacagtggtg aagcaatcgg cttgctgggc actgattctt tctaacgatt gcttggagat
       61 aacaccaaat tctctggcct tgctttcagg gtcaattttt tcgatgttgc tatacagcgg
      121 tatgttgtta ccagcaatac ttcgtcctag caccgtagca tgggttgtga ataccgttcc
      181 tacttgtgga acatttgctt ttaggtagag caatcctgtt ccggtcatcc actcgtgaaa
      241 ctgggctact atgcgatgtc gtaagcttag gttgaagttg gtaaaacttt caatcacctt
      301 gcctgctgca tagccaaata gggcgggttc aatatagtcc cattgtccgg ttatggaatc
      361 gagcttgaat ttttcccaaa agcctttgaa aatctcatct ttttttgata taagttggga
      421 aaagttgacc agaatagcta ccggtttaca gggtatattc catcggccaa cccttatggg
      481 cattccgttt tcaatggcat gactacgcca ggcggcaaat aggttggtgt cttctatgaa
      541 ttcagcatta tgctcaatcc cctttattag gtcggggcca acagtaatgt agttgttctt
      601 cagctcattt tcaatcatcg atgcctttgt tgacataacg gtgtgtatcc caccaacctt
      661 gttgcacacc tcccagctta cttcaaaaag gtaatcgggt tttagcagct tgtcattcat
      721 tcgttcacaa tatttttatg gtagtatttt gattttttcc aagtttaatc caaaataaat
      781 aggttaacca tttcatttgt tgctacaggt ttgtcattat ttggcagttt ctaccgatta
      841 acctactctt ttttcttttt tgttccctta gtggttgact ttgaattact tgatttggta
      901 ctggatttgg cagttgttgg gctacttttg atactgttct tcttttttga agatggcttt
      961 agcgctattt cttcagtttt ggtgcttaca acaggcttgc ttttaggtgt agttttactc
     1021 tttggtttag caacagcctt tgtagacttg ctgcttgttt ttgccttatt acctggtgtg
     1081 ctggcagcgt caggattatc aactttagcg ttgccagttt tgccggtagg ctttgcttgt
     1141 tgctcattta gctgattctt tagctcgtta attttatcct catagagttt aatgagcttt
     1201 tgggggttgt ctagttcagc tgatgattca tcgaccaatg ttttttcgag tagcttatcc
     1261 ttaaccctaa gggtaaagtc gcttaggatg ttcatgtagt tgatgaatgc ctcgtagggt
     1321 gaaccgtagg ggttgaagta cttgtgcaca tcgccatcgc taaaccattt ggtgcacatg
     1381 tagtagaagt ggtcgctggt ttggaggtaa agccagtccc ttttgatctc ggggtcatca
     1441 attttttcaa ccttatcggt aagcgcatat agttggttga aagcgctatc ctgcagctcg
     1501 ttgcccaacc atgcggtaag gtctctttcc tcgtcggccc aggaaatagg gtagggaacg
     1561 tgaagcaggg caaccggttg gtgtttctct gcagcctccg atggggtaag gaattcaaaa
     1621 ttggagtgac cgaaaaccct agatggcagg gcacgaagga aatcgaatat tccggtttca
     1681 gcccactggt gttcaccaaa ggtttcataa tccataaaaa ggttaacaat ttcctccttg
     1741 ggatcaatgt tgttgagcca ccaaacaaac ttttcggtgg ttagcggcca ctcgttccag
     1801 tcgcggttgg aaaagcggaa ggcgatgtca tcgcttagct ggaaattttt cagcagcagc
     1861 ttcaacttag gatttatagc gttggcatac acgaagttgg aacttttcca tcccagaata
     1921 tgcttggcgc cttcggtgag catggtttta aaccccaaat cggcaaccat ggcgccaatt
     1981 tcatcggaat atataagctc ggtgtttctg aaggttttgg gcttgtatcc aaggagtttc
     2041 tcaattttat tctgttgggc ttcaacctgc cggtagaatt cttccttgct cttgagcgag
     2101 gagagggagt gggagtaggt ctcggccaaa aactccacac ttccggtttt ggccaacctg
     2161 cgaaagctgt ccagtacgtc cggtgcgtat tgctcaaact ggtcgagcgc agtccccgaa
     2221 atggagtagg aaattttaaa ccttgtgcca aactcgttga ttaggtcgag cataagctgg
     2281 ttggtcggta ggtagcactt ttcggctacc ttccgcatga tggagcggtt ggcaaaatcg
     2341 tcgaagtagc tatggttttt cccaatatcg aagaagcggt accttcggag cctatatggt
     2401 tgatgcacct gaaagtagaa gcaaattgtt ttcatcagct caaaattttt aactttatag
     2461 taataagttg cggtatctac actgttgttt atgaccagct ggatgattca tgcctactgg
     2521 agtatatgtt ttcaagaatt tttcctgctt agcacatctt gatatgcttc aattaccttg
     2581 ctggcggcat tatcccactt caagctattc acctcttcct gtccgtgcct gataaaaaac
     2641 gtagataatg cattatagtt cagcaggcca taaatgctat cggcgagtgc atcaatatcc
     2701 cagaaatcaa cttttatggc gtatttaagc acctcagata cacccgactg cttggaaatg
     2761 ataacaggta cgctggaacg cattgcttct aatgggctta ttccgaaagg ctctgaaacg
     2821 gagggcatga cgtaaacgtc ggagaggtgg aacatcctgt tcacatccgg gcctttaaga
     2881 aatccggtaa agtgaaattt gtgggcaatg ccaagctggg ccacccgacg aatcatccgg
     2941 tttagcatat caccgcttcc agccataaca aagcgtacgt tatgagttcg cttgagcacc
     3001 ttgttggcag cttcaacgaa gtagtcggga cccttctgaa aagttaccct gcccaggaag
     3061 gtaactattt gctcggagac cccgcgctcg aattccatgt cctgctcatc ggagaagtcc
     3121 acagcgttat gcactgtaac caccttatct tctggtattc catagcgggt aattactata
     3181 ttgcgggtat agttactcac ggtgataacc ttgtcggcaa ccatcattcc cctttgctcc
     3241 acatcgaata ctgcctggtt gatgttctcc ccactcctgt caaattcggt ggcgtgaaca
     3301 tgcactacca ggggtttgcc cgaaaccatc ttggcggcaa tacctgccgg aaaggttagc
     3361 cagtcgtgcg agtggataac gtcgaacttt tcccttgcag ccaggttggc tgctacaaca
     3421 gcataccggg taacctcctg catcaggttg gggttgtatt ttccagaaaa cttaaaccgc
     3481 gtttttacct tgccggattc atactcaaca gtttctgcct cgttatgctc gataatttta
     3541 gcgtactctt cggggtcaac ataggggaca atgttggagc tgatctctag atacttaagg
     3601 tgcctccaga actcctccag gttcttggcg gagtaggcca catcaacatc ttcggcagca
     3661 accaacctca cagccgattt atcctcatcg ccgtaagctt ttggaacaac aaagatgacg
     3721 tcaacttttt gcttggtaag tccacgcgtt aagccaaagc aggctgttcc taaacctccg
     3781 gttatatggg gagggaattc ccatccgaac attaaaactc tcatacgcgt ttggttaatc
     3841 gctatacttc tcaatcattt ttacaattct gattagctcg gcaacgctcc atgcctgcga
     3901 tatgcagcca ttgggccgtt gtggtggatc gccgtcgtaa acctctgcca cggagcaaat
     3961 tccgtgtagt gcaatatctt cctgcagcga atcaactatt tcctgagcca atcctaaagc
     4021 gcctttaccc tgaagtttaa attgtgcttc aacgtaggct cctaaaagcc aaggccacgc
     4081 tgttccctga tggtaggcca tatcgcgtgt tggttgatcg ccctcatacc ttcccttgta
     4141 gtttgggttg ttcggcgaaa gggttcttaa tccttttggg gtgtaaagct catggcttac
     4201 aacgttgagc acggcaattt ttttctcatc atcaattggc gagtagggta aagaaactgc
     4261 aaaaagctgg ttgggcctga tgtcccagtt ttgtccattt tcgtcaacgt agtcggccag
     4321 gtagtttttc tctggaatcc agaatagctc gttgaatgat ttttttacct gatctgcaat
     4381 gggttgccat tctttcacaa accttaaatc tttatttttg gtagccagtt caagggtaaa
     4441 catcagggcg ttgtaccata gagcatttat ttcaaccggg tagccaatgc ggggggtaac
     4501 cggttttgcc tgaataacgg catccatcca tgtgatagcg tagccctttt caccggccca
     4561 aatcaagcca tttggctgca ttttaatact aaagggtagc ccttcgcgat aacctttcag
     4621 gatagccttc atttttttgc cataatcgtc ccacacttcg gtgtagtttt gtgtataact
     4681 ggcgtattgt tgaatggccc agaaatacca aagtggtgca tcggcggagt taaacgcttc
     4741 ggaattgcct gttctgacgt tggggaataa cccgtttttt agatcggtgc ttagcgagtc
     4801 gagcagtgct ttgcaggttt taacatcatc gagtccaagg gttaatccgg gtagtgaaat
     4861 gaacgagtcg cggctccagc tgctaaacca cggatagcca gcaataatat cgtagcgttt
     4921 tccccgttgg atgataaact ggcgcgctgt gttggcaatg cagttcatga agctgtcctt
     4981 gggtggtcgc cgggtttgct cggagttgaa tttacgcagt agggcagggg ttgactgttc
     5041 gttggtggca gccgaaaaaa ttatactttc tccctttctg atggaggttt caaagtatcc
     5101 gggcacaaat aggtcttcat gtccctcata tcctcttctg atttcctcca tatactccac
     5161 attgtagtac cagtcggggg atgcaacaaa ctcattctgc ctgttcaact gcatgtagag
     5221 gaacggaaag ccggtgtaga gtttatacct tacgccattg ctgcattctt ggtatttagt
     5281 gttagcccac atattcgcct tgctgaggct atgaacattt ctgaaagcca tgaatggctt
     5341 catccttaac tttgttgggc tatgagcatc cagcagggtg taacgtatca tcacctgctc
     5401 ctcattgtga acaaaaagca gctctttttt tagtattaca cc
//
LOCUS       DAOOQX010000041         5427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_18138_length_5427_cov_3.513217, whole genome shotgun sequence.
ACCESSION   DAOOQX010000041 DAOOQX010000000
VERSION     DAOOQX010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18138_length_5427_cov_3.513217"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLX53_01260"
     CDS             complement(<1..201)
                     /locus_tag="PLX53_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOY71739.1"
                     /translation="MNPDFKKVRINEVNAPKSPLSYNPEWVTSEKIPVMPAFSEADLE
                     SMEHLDYAAGIPPFLRGPYSTMY"
     gene            complement(361..2244)
                     /locus_tag="PLX53_01265"
     CDS             complement(361..2244)
                     /locus_tag="PLX53_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOY71740.1"
                     /translation="MVKKSNNERLFAEFPPVSTTEWEAAIAKDLKGADYEKKLIWKTN
                     EGFNVKPYYRSEDLADIQTVHTKPGEFPFVRGNNAKKNPWLIRQDMDVCLDKPTEANG
                     KALEWLQRGVDSVGFKLCSDCEPTFDSISRLLKGIDLNCVEVNFTGGVPGAKALPYIL
                     QYLEKGKFDIRQVRLSFDYSPLSNLTLNGKFCCEKDSSYANLQELVENVKSFPNVKVI
                     GINGNIFHNSGSSSVQELGFSLAMANDYIQFLLQKGYGIDDISPRIRFNFAVGTNYFM
                     EIAKFRAARLLWAKMVEVYKPTIETSARMSIHAETSRWSMTVYDPYINMLRTTTESMS
                     AVLAGVDSLNVLPFNAPYSEPTSFSERIARNQQILLREEAYFGKVADPAAGSYYIERL
                     TLSIAEHAWNLFLKVNEMGGYRDAFIKGYVQERISEVAAKRDANIATRRETILGTNQY
                     PNFNEVADLNVVTAEVVTRQNKLKPADAIAQPLTPYRGAQAFEELRYRTDKSGRRPKV
                     FMLTLGNLTMRRARAQFSSNFFAVAGFEILDNIGFNSVDEGVKTALDSQSEIVVLCSS
                     DDEYSELAPIAFEKLAGKTIFVVAGEPQCKSELESKGISNFISIKSNVLETLKGYQKM
                     LSI"
     gene            2480..3553
                     /gene="amrB"
                     /locus_tag="PLX53_01270"
     CDS             2480..3553
                     /gene="amrB"
                     /locus_tag="PLX53_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOY71741.1"
                     /translation="MKKLSIFAIVLASMVACTSSKSPKSVRFFADTVGFASKATQMDS
                     VYARIFRFQGDSLNRKQALPGARLVICPHDDYAYVGWQYPATLRNITAKTVIILGVAH
                     KAKTFNIENKLVFDSFDCWHGPYGDVKVSPIRDELLRLMPADMVVVHDSLQLAEHSVE
                     PMIPFLQRSNPNIEIVSILVPYMPFARISELANSLAQNIYSISSKKHLEWGKDFAFII
                     STDAVHYGDEGWGGNNYAPFGADSAGYAKAVEHEYQIVDSCLAGKATPERIERFYRYT
                     VSDADYREYRWTWCGRYCVPLGLATAIRLSNLSKGSSLEGKLVSYSTSIDHQPLLVED
                     LGMGRTAPASIRHWVGYASLVYK"
     gene            3880..4966
                     /gene="prfB"
                     /locus_tag="PLX53_01275"
     CDS             join(3880..3942,3944..4966)
                     /gene="prfB"
                     /locus_tag="PLX53_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132894.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOY71742.1"
                     /translation="MITAEQVKDIVRRRDALRRHLDIDLKQVELDEEEQKTQYPGFWD
                     NPSEAESQLKKIAGIKIWTNGFSKVNSLVEDLLVLYDFAKQGDATDVELEEHFNSTLK
                     SIEELELKNMLRKEEDNLGAIMKINSGAGGTESMDWAAMLMRMYMRWGERNGYDVKVL
                     DIQEGEEAGIKSATLEFTGEMAYGYLKSENGVHRLVRISPFDSNARRHTSFASVFVSP
                     AIDDRIDVVVNQGDIKWDTYRSSGAGGQNVNKVETGVRLYHIPTGIVIENTESRSQFQ
                     NKENAMRLLKSQLYELELRKRREAQAEIEGSKKKIEWGSQVRNYVLHPYKLVKDLRSN
                     YETSDVNAILDGDLNECIKAYLMQYGE"
     gene            5010..>5427
                     /gene="rsgA"
                     /locus_tag="PLX53_01280"
     CDS             5010..>5427
                     /gene="rsgA"
                     /locus_tag="PLX53_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HOY71743.1"
                     /translation="MFLGVVVKSTGNLYRVKLEDGSTVECTVRGKLRLKGSKTTNPLT
                     VGDRVNVETSHEGTAVIVDILPRTNYIIRRSTNLSRESHIIAANIDQAILVVTIIQPE
                     TQTAFIDRYLVTSEAYSIPAILVFNKVDISFLAEAEI"
BASE COUNT         1524 a         1178 c         1230 g         1495 t
ORIGIN      
        1 gtacatggtg ctgtacgggc ctctcagaaa tggtggaata cctgccgcat agtccagatg
       61 ctccatactt tccaaatcgg cttctgaaaa agccggcata accggtattt tttccgatgt
      121 tacccactcc ggattgtagc ttaatgggga ttttggggca tttacctcat taatcctaac
      181 ttttttaaaa tctgggttca tccctgtagt gaatttactt tttaccaatt tcgttttaaa
      241 aacttttcaa ttattgatta tccaacctga aggctcaacc agaaattcct aatgtattat
      301 gatataaaaa taacgcactt taaacctaca acggatggct aatcgccgga aaagcagggt
      361 ttagattgac agcatcttct ggtagccttt caaggtttca agcacattac tttttatgct
      421 gatgaaattg ctaatccctt tagactcgag ttcggatttg cactgcggtt ctcccgccac
      481 tacgaaaatt gtctttccgg caagtttctc aaaggcaata ggggcaagtt cagagtactc
      541 atcatcggag ctgcatagca ccactatttc tgattgtgaa tcaagtgctg tcttaacacc
      601 ctcgtcaaca gaattaaagc caatgttgtc cagaatctca aatccggcaa ctgcaaaaaa
      661 gttagagctg aattgggctc ttgcccgccg catggtgagg ttgccaaggg tcaacataaa
      721 gactttgggc cgccttccgc ttttgtcggt tctgtagcgt agctcctcga aagcctgcgc
      781 tccccggtac ggagtaaggg gttgggctat tgcatctgca ggtttaagct tgttttgacg
      841 ggtaacaacc tccgctgtta ctacgttcaa atcggctacc tcgttgaagt tggggtactg
      901 gttggtgcct aaaattgttt cacgtcgggt ggctatattg gcgtcacgct tagccgcaac
      961 ttcgctaata cgctcctgaa catacccctt gataaaggca tcacggtagc cacccatctc
     1021 gttaacttta aggaaaaggt tccatgcatg ctcggcaatg gaaagggtta acctctcaat
     1081 gtagtaactc cccgccgcag ggtcagcaac tttcccaaaa taggcctctt cgcgtagtaa
     1141 aatttgctga ttccttgcaa ttctttcgga aaatgaggtt ggctcggaat agggggcgtt
     1201 gaatggtaaa acgttcagcg aatcaacacc tgcaagaacg gccgacatgc tttctgttgt
     1261 tgtgcggagc atatttatgt atgggtcgta aaccgtcatg ctccaccgac ttgtttcggc
     1321 atgaatactc atgcgagcag atgtctcaat agttggcttg taaacctcaa ccatttttgc
     1381 ccaaagcagc ctggccgctc taaatttggc aatctccatg aagtagttgg tgccaacagc
     1441 aaagttgaag cgaatacgtg gcgaaatatc atctatacca taaccttttt ggagtagaaa
     1501 ctgaatatag tcgttggcca ttgccagcga aaaacccaac tcctgaacgg aagacgagcc
     1561 tgaattgtgg aaaatatttc cattgatgcc tatcacctta acattgggga aacttttgac
     1621 gttttcaaca agctcctgca agttggcata gctggagtcc ttttcgcagc aaaacttacc
     1681 gttgagtgtt aagttggaaa ggggactgta atcgaatgag agtcgtacct gacgaatatc
     1741 aaacttccct ttttcgaggt actgcaaaat gtacggcagc gcttttgctc caggaactcc
     1801 ccctgtaaag ttaacctcaa cgcagttcag gtcaattccc tttagcaacc tgctaataga
     1861 gtcaaaggtt ggttcgcagt cgctgcaaag cttaaagcct accgagtcca cccctcgttg
     1921 tagccattcc aaggctttgc cgtttgcttc ggttggctta tcgaggcaaa catccatatc
     1981 ctgacgtatt agccagggat ttttcttagc attatttccc ctaacgaagg gaaattctcc
     2041 gggtttggta tgaacggttt gaatatccgc taaatcctct gaacggtagt agggcttaac
     2101 attaaacccc tcgtttgttt tccaaataag cttcttttcg tagtctgctc ctttcagatc
     2161 cttggctatt gccgcctccc attcagtggt tgaaacaggt ggaaattcgg caaaaagcct
     2221 ttcgttgttg ctctttttta ccataatcgg actagttgga ttattgattt gcaaatttag
     2281 caattactga attggtaaaa cgctgcatag taacatattt gcctttacca tgcttttaag
     2341 cataatttga aagttctttt tatccattgc tattgaggtt ttaatggaaa attatttact
     2401 tgcatcctgg tgaaaatgtt taaggtttaa ctattttctc tcatgaaatt ccccacaatt
     2461 ttacctttaa ggttgaagta tgaagaagct atcgatattt gctatcgtgc tagctagcat
     2521 ggtggcttgt accagcagca aaagtcctaa atcggttcgc ttttttgccg atacggttgg
     2581 ttttgcctca aaggcaactc agatggactc ggtatatgct aggattttca ggtttcaggg
     2641 tgatagtctg aatagaaagc aagcattgcc cggagccagg cttgtaattt gccctcacga
     2701 cgattacgcc tacgtaggct ggcagtaccc agcaacgtta agaaatatta ccgcaaaaac
     2761 agtaataatc cttggcgttg cccacaaagc taaaactttc aacattgaga ataagctggt
     2821 tttcgacagt ttcgactgct ggcatggccc ctacggagat gttaaagttt cgccaatcag
     2881 agatgaactt ctcaggctaa tgcctgccga tatggtggtg gttcacgata gccttcaact
     2941 cgctgagcat tcggtagagc ccatgattcc atttctgcag cgcagtaacc cgaacatcga
     3001 aattgtttcc attcttgttc cgtatatgcc cttcgcaaga atttccgagc tggcaaatag
     3061 cttagcccaa aacatttata gcatttcatc taaaaagcac ttggaatggg ggaaagactt
     3121 tgctttcatc atctcgaccg atgccgttca ttatggcgac gagggttggg gtggaaataa
     3181 ttatgcccca ttcggggcag attcagccgg ctatgccaaa gcggtagagc atgaatatca
     3241 aatagttgac agctgcttgg ccggaaaagc aacgcccgaa cgtattgaaa gattttatcg
     3301 ctacaccgta agcgatgccg attacaggga gtatcgctgg acctggtgcg gcaggtactg
     3361 cgttccactt ggactggcaa ctgccattag gctttccaac ttaagcaaag gttcaagcct
     3421 ggaggggaaa ttggtttcat actcaactag catagaccac cagccgctat tggtagaaga
     3481 tttgggaatg ggcagaacgg ctcctgcaag cataagacac tgggtaggtt atgcctcgtt
     3541 ggtttacaag tagccattgg aggtttgctt agccattctt ttgaataagc taataaacat
     3601 aggtttaggc aaaatacttg cttgattaag tgtgttattt caccgatgga agaattgcat
     3661 aaccctcatt tttttgcaga gatctagcct tactccaaaa ttcggctacc ctaacttttt
     3721 gaaatcggat ttaaccagct ccgctctatg gcatcataaa gaatatactg tatttactcg
     3781 agacttactc acagaaaaac tataggggaa atccttcttc aaattactaa tccattcaac
     3841 ctttgcttca atatatgcta tatttgcaga aaaaagtata tgattactgc cgaacaagtt
     3901 aaagatatag taaggcgacg ggatgcgcta aggaggcatc tttgacatcg atttgaagca
     3961 agtagagctg gacgaggaag agcagaaaac tcaatatccc ggtttttggg ataacccatc
     4021 agaggcggaa agccaactaa aaaaaattgc aggcatcaaa atatggacaa acggattcag
     4081 caaggttaac agccttgtgg aggatttgct tgttctctac gatttcgcca aacagggcga
     4141 cgctaccgac gtggagctgg aagagcactt caattctacc ctgaaaagca ttgaggagct
     4201 tgaactcaaa aatatgctac ggaaggagga agacaacctg ggggctataa tgaaaataaa
     4261 ttcaggagcc ggtggaaccg aaagcatgga ctgggctgcc atgctgatgc gaatgtacat
     4321 gcgatgggga gaacgaaatg gttatgatgt taaggtgctg gatatccagg aaggcgaaga
     4381 agccggaata aaatcggcta ctctggaatt tacaggtgaa atggcctatg gatacctgaa
     4441 aagtgaaaat ggcgttcacc ggttggttag gatttcccca ttcgacagca atgcccgtcg
     4501 ccacacatcg tttgcttctg tttttgtttc tcctgcgatt gacgatagga tagacgttgt
     4561 agttaaccag ggcgatataa aatgggatac ttaccgctcg tcgggtgcgg gtggtcagaa
     4621 tgtaaacaag gtagaaacag gtgttagact ttaccatatc cccacaggaa tagtgattga
     4681 aaacacggaa tcccgttccc agttccaaaa caaggaaaat gccatgcgct tgctcaaatc
     4741 ccagctttac gagcttgagc taaggaagcg tagggaagca caagcggaaa tcgaggggag
     4801 taagaaaaaa attgaatggg gttcgcaggt aagaaattac gttctacatc cctataagct
     4861 ggttaaagat ctccgctcaa actacgagac atccgatgta aatgccattc tggatggcga
     4921 tttaaacgaa tgcatcaaag cttacctgat gcagtacgga gaatagggag aaagtagtgc
     4981 ccatagtgaa ataatttatt gttcagacta tgttcttggg tgttgtagta aaatcaacag
     5041 gaaatttata ccgggtcaag ctcgaagatg gcagcacggt tgaatgtact gttaggggca
     5101 agctccggct aaaagggtct aaaacgacca acccgctaac tgtaggcgat agggtaaatg
     5161 tggaaacaag ccatgaaggt actgccgtaa ttgttgacat cctgcccaga acaaactaca
     5221 tcattcgccg ttcaaccaac ctttcaaggg agtcgcatat cattgctgca aacatcgacc
     5281 aggcaatcct ggttgtaacc attatacaac ccgaaacaca aaccgctttt attgatcgct
     5341 acctggttac atcagaggca tactcaatcc ccgcaattct agttttcaat aaagttgaca
     5401 ttagcttcct tgccgaggcg gagattc
//
LOCUS       DAOOQX010000042         5416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_18176_length_5416_cov_3.210035, whole genome shotgun sequence.
ACCESSION   DAOOQX010000042 DAOOQX010000000
VERSION     DAOOQX010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5416
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18176_length_5416_cov_3.210035"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLX53_01285"
     CDS             <1..226
                     /locus_tag="PLX53_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY71744.1"
                     /translation="NLYPNPASDYINISLTGASSEVTVRIFDVRGSMVKSQVLNASEN
                     RIDVSTLAKGIYMVSVGEGKEISTKRFIKQ"
     gene            complement(532..1710)
                     /locus_tag="PLX53_01290"
     CDS             complement(532..1710)
                     /locus_tag="PLX53_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOY71745.1"
                     /translation="MRRISLIISNLLLFVAIANAQVSIEKILGEVERNNTTLVALRQK
                     MEAEKIGSRTGLYPQNPSFEYSYQWGNPADIGSKTDISLIQSFDFPTAYLYRSKIAKA
                     EIELLATEYLRQSKQLLLDVRLVCIELVYQNALSSVLGKRVSNAKLIADAYSKKLDVG
                     EASALENNKARLTLLNATQELFSVKLKQNELLAKLASLNGGIPLELSDSVFNMPAIPA
                     SFDEWYAATEQSNPDLKAIEREIAVAQRNIQLSRALCLPQIKAGYASEKVAGEHFQGI
                     AAGISIPLWENSKRVKYAKARAQYLRSVEADRKVEIFSRQQAQYNTALELQKNIAEYR
                     NSMGTINSSRMLDEALQKGEISLVDYLLEESLYYSSAETLLQMERDFCRVVAELYQYQ
                     "
     gene            complement(1707..4808)
                     /locus_tag="PLX53_01295"
     CDS             complement(1707..4808)
                     /locus_tag="PLX53_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOY71746.1"
                     /translation="MLDRIIKFSLSNKYTILIAFFVLAVVGIRTARNMDVDVFPDLTA
                     PTVVVMTDAHGMASEEVERLVTFPIETAINGATDVRRVRSVSSQGFSFVWVEFDWGSD
                     IFKARQIVSEKLITVSSQLPIGVGQPVLAPQSSVMGEIFFVGLTADSTSMMDLRTIAE
                     WEVKPIILATNGVSQVTIIGGDFKQYQVLADPVKMMSYGVSMDELATVCRNISGNSTG
                     SNIRQFGNEYAVRGMARASDLETLGNSFIKFLNGHPVRVSDVAEVVIGSAAKMGYASE
                     NTKPAVIISISKQPNANTLKVTENIEKNLDNIYKTLPKDVRLDTGIFRQADFIQTSVG
                     NVQNALMEGAIFVVIVLFLFLGSFRTTIISLLAIPLSLLGTIVALKAFGLTINTMSLG
                     GMAIAIGSLVDDAIIDVENVFKRLRQNNLKPVDERQSSFTVVFEASREIRASILNATL
                     IIIVAFLPLFFLSGMEGRMLKPLGISFIVSLTVSLVVAMTLTPLLSKMLLTNESYLAK
                     NEREKWLSRNLQTYFKASIEWVLRHKKVVLGAVLSLVILSIVAFASMGRSFLPEFNEG
                     SLTLSVVTKPGTSLDETNKLGNLVEQQLLAIPEIYSTARRTGRGELDEHSQTVNSSEI
                     DVNFKLSERSRDEFVANVRQTLAGIPGIAATVGQPLGHRIDHMLSGTRANIAIKLFGT
                     DLNQMFAIGNQIKGEISGIEGLVDVNVDQQVDIPQIQLKANRDMLAAYGIPVGSFNEF
                     IDVAFGGEKLADIYEGQRRFDLVLKLNHRYTESIEGIGVALIDTYNGGKVPLSEVAQI
                     QSVSGPSSISRENAQRKVVVSANVAGRDMRSVVNDIQDRVNSHITLPEGYRIEYGGQF
                     ESEANASRTLMITSILAILVIFLLLFQEFRNFKLAAIILLNLPLALIGGIFSIYITGG
                     ILSIPAIIGFITLFGIATRNGILLVSKFQQLQAQGLPIHDTILKGSTDRLNAILMTAL
                     SAALALIPLAMRGHLPGNEIQSPMAKVILGGLITSTILNIYIVPIAYSLLAKRGLTKT
                     DKQ"
     gene            complement(4820..>5416)
                     /locus_tag="PLX53_01300"
     CDS             complement(4820..>5416)
                     /locus_tag="PLX53_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOY71747.1"
                     /translation="NVSSPIDGYVQDISVQNGQYVEAGQPVLTVFGSRFYVLNAGVQQ
                     KYFKSLEGLKTATVKQANGSYVGIDKFNGRIMSVGNSALAGNGYLIPVIIQVDATDRL
                     VVGSFVELNLIAKSDSMLLTVPTSAILEEQGNYYVMVQLNPETFEKRQVQLGVSDGLR
                     VEIRSGLLSTERVVTRGAVLVKLAQVSGKLDAHAGHVH"
BASE COUNT         1514 a         1310 c         1169 g         1423 t
ORIGIN      
        1 caacctttac cccaacccgg caagcgatta catcaacatc agtctaaccg gtgctagtag
       61 cgaggtaaca gttcgtatct tcgatgtacg cgggtcgatg gttaaatcgc aggttctcaa
      121 cgcaagcgaa aaccgaattg acgtaagtac cctggcaaaa ggtatttaca tggtgtcggt
      181 aggcgaaggt aaagagattt ctaccaaacg ctttattaag cagtagtagg aaactatcca
      241 gtcataaaaa gttgaaaaag ccgtggtagc ttaccacggc tttttttaat atcaacagca
      301 aacaaagctg caattgcctt agacagaaat tgctggcaag ttagaataat tccacatggg
      361 catactcccc ttgttccccc ggttatgaac atgagcaact ttcctgagaa tctgaggttc
      421 aaggttccaa tacatgaaaa ttgctacagc tgctaaattt gagggatttt accatttagt
      481 gttgaaaata gcaaaaattt aaagctacgg ttcgaaagtc aaatttttgg atcattggta
      541 ctggtatagc tcagcaacaa ctctgcaaaa atccctttcc atttgaagca atgtttcagc
      601 gctactatag taaagcgatt cctccagcag gtaatcaaca agcgaaattt cacccttttg
      661 aagcgcttcg tccagcatcc tactgctatt aattgtgccc atgctattcc tgtattctgc
      721 tatgtttttt tgcaactcca gagcagtatt gtattgcgct tgctgcctac taaaaatttc
      781 aaccttacgg tcggcttcca cactcctaag gtactgggca cgtgcctttg catactttac
      841 ccttttggaa ttttcccata agggtattga aattcctgca gcaataccct gaaaatgctc
      901 accggcaacc ttttcactag cgtatcccgc tttaatctga ggtaaacata acgccctgct
      961 tagctggata ttcctttgtg ccaccgcaat ctcccgttca attgccttaa gatccggatt
     1021 agactgctcg gtggcagcat accactcatc aaaacttgca ggtattgcgg gcatgttgaa
     1081 aacgctatcg cttaactcaa gcggtatgcc accatttagg ctagctagtt tagcaagtag
     1141 ctcgttttgc ttaagtttaa cgctaaacaa ctcttgtgtg gcattaagca gggtaagacg
     1201 agccttgttg ttctcaagtg cgctggcttc tccaacatcc aacttttttg aataggcatc
     1261 ggctattagc ttagcattgc tgacacgctt ccccagcact gatgaaaggg cattttggta
     1321 aaccaactct atacatacca gcctaacgtc cagcagaagt tgtttactct gacgaaggta
     1381 ctctgttgca agcaattcta tttcagcttt agcaattttt gaccggtaaa ggtatgctgt
     1441 agggaaatcg aacgattgaa tcaggctaat gtccgtttta ctgcctatat cagcaggatt
     1501 gccccactgg taggaatact caaagctggg gttttgaggg taaagtcccg ttctgctgcc
     1561 aattttttcg gcctccatct tctgcctgag agcaacaagg gtggtattat ttcgctctac
     1621 ttcacccaaa atcttctcga tggatacctg tgcgttagct attgcaacaa acaacaaaag
     1681 gttggaaatt atcaggctta ttcttctcat tgcttatcag ttttagtcaa accccgtttg
     1741 gccagcaagc tgtatgcaat aggcacaatg tagatattta agatggtgga ggtgattaat
     1801 cctcctagaa taaccttggc cattgggctt tgaatttcgt taccagggag atgccccctc
     1861 atggcaagag gaattaaagc caatgctgcc gacagggcgg tcatgagtat agcattcaac
     1921 ctgtcagttg aaccttttaa tatggtatcg tggattggta agccttgggc ttgcagctgc
     1981 tggaatttgg ataccagcag aataccatta cgggtggcaa tgccaaaaag tgttataaac
     2041 ccaataatgg cgggtatgct taaaattcca cctgttatat agattgagaa aattcctcca
     2101 atgagtgcca gtgggaggtt gaggaggatt atggcagcca acttaaagtt tcgaaactcc
     2161 tgaaaaagca acagaaaaat aacaagaatg gccagtatgg aggttatcat tagtgtccgc
     2221 gaagcattgg cttcgctttc aaattgaccg ccatactcaa tcctgtatcc ctcgggcaag
     2281 gtgatgtggc tattcacccg atcttgaata tcgttcacca cacttctcat atctcgtcct
     2341 gccacgttgg ccgacaccac caccttgcgc tgagcatttt cacgactaat ggaacttggg
     2401 cccgaaactg actgaatttg agccacctcc gataaaggca ctttacctcc attgtaggta
     2461 tcaattagcg caacgccaat accctcgata ctttccgtgt agcggtgatt caacttcaac
     2521 accaaatcga aacgtcgctg gccttcgtaa atatctgcca gcttttcgcc accaaaggca
     2581 acatctataa actcgttaaa gctgccaaca ggaatgccgt aagcggcaag catatcccgg
     2641 ttggctttca actgaatctg tggaatgtca acctgctgat caacattcac atcaaccagt
     2701 ccctctatcc cggaaatctc acctttaatc tggttgccaa tggcaaacat ttggttaagg
     2761 tcggtgccga aaagtttaat ggcaatgttg gcacgagttc ccgagagcat gtggtctatt
     2821 ctgtggccca aaggctgacc aactgtggct gcaatacccg gaatgccggc aagggtttgg
     2881 cgaacgttag caacaaactc atcgcgggag cgctctgaca gtttgaagtt tacatcaatc
     2941 tccgagctgt taaccgtttg ggagtgctcg tcgagttcac ccctacccgt tctgcgagcc
     3001 gtactgtaga tttcgggaat ggcaagcagc tgctgctcaa ccaggttgcc tagcttgttg
     3061 gtttcgtcaa gcgatgttcc gggtttggtt accaccgaca gggttagcga cccctcgtta
     3121 aactccggta ggaaacttcg gcccattgag gcaaaagcaa ctatggagag aataacaagc
     3181 gacagcacag ctcccaacac aactttttta tggcgaagaa cccattcgat cgatgcctta
     3241 aagtaggttt gcagattacg tgaaagccac ttctcccgtt cgtttttggc caggtaactc
     3301 tcgtttgtta gcagcatctt tgataaaaga ggggtaagtg tcatggcaac caccagggaa
     3361 acggtaagcg aaacaatgaa tgaaatgcca agaggtttaa gcattcgccc ttccataccc
     3421 gacaggaaaa acaatggcag aaaggcaacg ataatgataa gagtagcgtt tagaattgaa
     3481 gctcgtatct ccctggaagc ctcaaaaaca acggtaaagg atgattgtct ttcatcaacc
     3541 ggcttgagat tgttttggcg caggcgttta aatacgtttt caacatcaat tatggcatca
     3601 tcaaccagcg aaccaatggc aatagccata ccacccagac tcatggtgtt gatggtaagg
     3661 ccaaaagcct taagtgcgac aattgtacca agcagcgaaa ggggtatggc cagaagagaa
     3721 ataatagttg tgcggaagct gcccaggaaa aggaacagca caatcaccac aaaaatagca
     3781 ccttccataa gggcgttctg aacattgcca acagaggtct gaataaaatc ggcctgacgg
     3841 aaaattccgg tatcaagcct aacatcctta ggaagagttt tgtaaatgtt atcgagattt
     3901 ttctcaatgt tttcagttac cttaagggta ttggcattgg gctgcttgga tatggaaatg
     3961 atgactgcgg gctttgtatt ctccgaggca taacccattt tggcagcact tccaataaca
     4021 acctcagcaa catcacttac cctaacggga tgcccattca gaaacttgat aaaggagttt
     4081 cccaacgtct ccaagtcgga ggcacgagcc attcctctca ccgcatactc gttgccaaac
     4141 tgacggatat tactgccggt agagttaccg ctaatgtttc ggcaaactgt ggcaagctcg
     4201 tccatgctaa caccgtagct catcatttta acaggatcgg ctagcacttg gtactgctta
     4261 aaatctccac caataatggt tacctgcgac actccattag tagcaagaat aataggttta
     4321 acctcccatt cggcaatggt tcgtaaatcc atcatgcttg tgctatcggc tgttaaacca
     4381 acaaagaaaa tttcgcccat cacgctcgac tgtggggcaa gcaccggttg accaacgcct
     4441 attggtagct gggaactaac agtgataagc ttttcgctta caatttggcg agctttgaaa
     4501 atatcgctgc cccagtcaaa ttcaacccaa acaaacgaga atccctggga ggaaaccgag
     4561 cgaacgcggc gaacatccgt tgccccatta attgccgttt cgatgggaaa agttaccagc
     4621 cgttccacct cctccgaggc catgccatgg gcatcagtca tcaccactac ggttggtgct
     4681 gtaagatcgg gaaaaacatc cacatccatg ttgcgggcgg tgcgaattcc tactaccgcc
     4741 agcacgaaga aagcaataag tatggtgtac ttattgctca gcgagaactt aataatccta
     4801 tccagcatag cgcatcagct taatgaacat ggcccgcgtg ggcatcaagt tttcctgata
     4861 cctgtgccag ctttaccaaa accgccccgc gggttacaac acgttcagtg gaaagaaggc
     4921 cagacctaat ttctacccta agcccatcgc ttaccccaag ttgaacctgc cgtttttcaa
     4981 acgtttcggg gtttagctga accataacat agtagttgcc ctgttcttcg agaatggccg
     5041 atgtgggtac agtcagcagc atactatccg acttggcaat caggtttagc tcaacaaaac
     5101 ttcccacaac aagcctgtcg gtagcatcta cctgaataat caccggaatc aggtagccat
     5161 taccagccaa ggccgaattc cctaccgaca tgatcctgcc gttgaactta tctattccaa
     5221 catagctgcc attggcctgt tttaccgtag cggtttttaa accctccaaa cttttgaaat
     5281 atttttgctg aactccggca ttgagcacat agaatctgct gccaaaaact gttaaaacgg
     5341 gttgaccagc ctcaacatac tgtccatttt gaacggaaat gtcctgcaca tacccatcaa
     5401 taggcgaaga aacgtt
//
LOCUS       DAOOQX010000043         5345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_18477_length_5345_cov_4.244045, whole genome shotgun sequence.
ACCESSION   DAOOQX010000043 DAOOQX010000000
VERSION     DAOOQX010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18477_length_5345_cov_4.244045"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PLX53_01305"
     CDS             complement(<1..535)
                     /locus_tag="PLX53_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71748.1"
                     /translation="MLISIELFSQVKDPVDYVNPFIGTDFHGHTYPGATVPFGMVQLS
                     PDTRLTGWDGCSGYHYSDRVIYGFSHTHLSGTGASDYGDILIAPVSGKPVFENTRYSS
                     PFKKENEMAVAGYYSVFLDKYKVQAELTVTRRVGFHRYTFKNPNKAALVIDLEHRDRV
                     IESWLKVVGKNELHGFRR"
     gene            complement(771..1898)
                     /locus_tag="PLX53_01310"
     CDS             complement(771..1898)
                     /locus_tag="PLX53_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOY71749.1"
                     /translation="MSYFDDSRIVLTLDAGGTNFVFSALQGIKPIADTITLPSNGNNL
                     EKCLQGIVEGFTKLGAKLKGKPSAISFAFPGPADYPKGIIGDLGNLPGFRGGVALGPM
                     LQEKFGIPVFINNDGDLFAFGEAIAGFLPWVNSMLKNAGSPKNFYNLLGVTIGTGLGG
                     GLVTNGKLFTGDNSAGAEVWLLRDGVNPTCFAEEEISIRAVQRTYHAHTRKNESTLLS
                     PKEIAEIAYGKHEGDMVAAKLAYQSLGASLGDALANIITITDSIVVVGGGLSNAYHLF
                     APAMLAQLNGSISTVTGEQVNRLESKVFDLENEGQCNLFVKGENRKIKVPLSSKEVDY
                     DPLKRVGIGVSRLGTSHAVAIGAYVYAINMLDHNTLHSQAI"
     gene            complement(1895..3043)
                     /locus_tag="PLX53_01315"
     CDS             complement(1895..3043)
                     /locus_tag="PLX53_01315"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY71750.1"
                     /translation="MNRKTFTILLAFLCMGFGDAVGPFVGLAKEQFELSNFEAQLIAF
                     MGFIMFGFLSVPMGIFQDRRGKKVVLMLGLAVALAGLTIPLFGLGSYALFLLTILLLG
                     AGAAILQVAGNPIMRDVSEPGMYSRNLSFGQFIKAIGSLSGSIIPFAAAKWMGADWQV
                     LFPIYVSALLLTLVAVGFTKIEEEKSDKKPATLNSCFSLLKNRFVLLMVLGIFIYVGI
                     EICLSSGVPILLSTHFGIDLKTWGLLGNAFFFIAILIGRLLGSIILSWTKPAAFFRVS
                     CLIALMGILLIFSNNQITTLVGIFTTGLGFANIFPLIFSITVDAMPERANEISGLMVT
                     AIAGGAFIPPVMGLVADMSSVRTGFLVPLLGLGFIIVLSFLKPQPSRQ"
     gene            complement(3401..4447)
                     /locus_tag="PLX53_01320"
     CDS             complement(3401..4447)
                     /locus_tag="PLX53_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOY71751.1"
                     /translation="MKKVTLNDIAKKVGVSKTLVSLVVNGKARGYGISGQTCDKVMAA
                     VRAYGYNPNQTARSLRTGMSNTLGLIVSDISNNFYASLARQLEDLAWELGYNMIIGST
                     DESVGKELKLLEIFRNRQVDGIILSSSQQNPTELETLVKSGFPLVLIDRYFPTVPIPS
                     AVVNNKVGGELLAQHLLNQGFRNPVVLSLTPSYISSVAERVEGFVSVFENAAIHTEVH
                     NIPFLCLVDMVEDFFTTRLHDKNMPDAVFAVNNNLATATLRACHVHNIVIPKDIALVC
                     FDELPYFDFSKPSITSIRQPVAELCSVAFSLLLGQITNNGETKPGHVEMMPVELLVRE
                     SSTQISDNNGWRSP"
     gene            4754..>5345
                     /locus_tag="PLX53_01325"
     CDS             4754..>5345
                     /locus_tag="PLX53_01325"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY71752.1"
                     /translation="MRKLISAILAILPMFTTFGQRSDIDSLLKQLNEAPNTSVKAETA
                     IDLAQRYINIDTDSAMAFNQIAHQLATDLSDTALMIRCENTYGFIFNVNSSFEKARER
                     FSRVLEMAISAHDSSLIASAYGNIGNTYMFEQKYSMAVESFLKALDIFEKRNNQKGVA
                     TVYGVLGNLYLSLDKLDQSLEYYQKAQSMFHTLGWAD"
BASE COUNT         1517 a         1193 c         1197 g         1438 t
ORIGIN      
        1 atcggcgaaa cccatgcagc tcgttcttcc ctactacctt taaccaagat tcgatgaccc
       61 tatcgcgatg ctcgagatca ataaccagcg ccgctttatt gggattctta aaggtgtagc
      121 ggtggaagcc aaccctacgg gttacagtaa gttcagcctg aaccttgtac ttgtcgagaa
      181 aaaccgagta gtaaccggca accgccattt cattttcctt tttaaatgga gaagaatacc
      241 gcgtgttttc aaagacgggt ttgccagaaa caggggctat gagaatatcg ccataatcag
      301 aagccccggt accgctaagg tgagtatgag agaacccata gattacccta tcggagtaat
      361 gataccccga acagccatcc caaccggtta gccttgtatc gggactaagc tgaaccattc
      421 caaagggaac ggttgccccc ggataggtgt gtccgtgaaa atcggtacca ataaatggat
      481 taacgtaatc cacggggtct ttcacctgtg aaaacagctc aatgctgatg agcattacaa
      541 ctgtcaatag ttgcggcttt ttcattgttt ataggtattt ataagtggtg ttattttatt
      601 atggcctagt ttacttttat tccaaacttg ttggtgtttg cattttttgt ctaataaaac
      661 caacaccctg aaacatgggg tggggattac tcctattacc ttggatttgc tttcatacat
      721 tagcattaag cgttcgcctt aaatttatta gatgatggag gattaaagca ttaaatagcc
      781 tggctatgta atgtgttatg gtcaagcatg ttaatggcat aaacataggc tcctatagca
      841 acggcatggc tggtccctaa acgtgaaacg ccaatgccta cccgtttaag aggatcgtag
      901 tcaacctctt tgctgcttag gggaactttg attttccgat tttcaccctt aacaaaaagg
      961 ttgcactgcc cttcattctc aaggtcaaac acctttgact ccaagcggtt tacctgctct
     1021 ccggtaactg ttgaaatgct tccatttagc tgggcaagca tggcaggggc aaaaagatgg
     1081 taggcgtttg acaatccacc tccaacaact acaatcgaat cggttatagt tatgatattt
     1141 gccagagcat cgccgaggga ggcgccaagc gactggtagg ctaacttggc agcaaccata
     1201 tcgccctcgt gttttccgta ggctatttca gcaatttctt taggtgaaag cagtgtgctt
     1261 tcatttttcc tcgtatgtgc atggtatgtt cgttgcactg ccctaatgct gatttcttcc
     1321 tcggcaaagc aggttgggtt gacaccatcg cgcagaagcc aaacttctgc tccggcagag
     1381 ttatcgccgg taaaaagctt accattcgtc accagaccgc ctcctaaacc cgtgccaata
     1441 gttactccaa gtaggttgta gaaatttttt gggcttccgg catttttaag catgctgttt
     1501 acccagggca gaaaacctgc gatggcctct ccgaaggcaa agagatcgcc atcgttattg
     1561 ataaagacag gaattccgaa cttctcctgt agcattggtc ccagggcaac gccaccccgg
     1621 aatcctggaa gattccccaa atcgccaata attcctttgg gatagtctgc aggtccgggg
     1681 aaggcaaagc tgatggccga aggtttccct tttagcttag ctcctagttt ggtgaaccct
     1741 tccacgattc cctgaagaca cttttcgagg ttgttaccgt tgctgggtag tgtaattgta
     1801 tcggcaatgg gttttatccc ctgtagagcc gaaaatacga agttggttcc gcctgcgtca
     1861 agggttagca caattctgct atcgtcgaag tagctcattg tctggagggt tgaggtttta
     1921 aaaatgatag aacaataata aatccaagac ccagcaaagg aaccaaaaag cctgttctaa
     1981 cgcttgacat atcggccacc aagcccataa ctgggggaat aaatgcgcca cccgcgattg
     2041 cggtaaccat taaacccgat atttcgttag cccgctctgg catggcatcg accgtaattg
     2101 aaaaaatcaa agggaaaatg tttgcaaacc caagaccggt agtgaatatt cctaccaatg
     2161 tggtaatctg gttgttggaa aaaatgagca ggatacccat tagcgcaatg agacatgaaa
     2221 ccctgaaaaa tgctgccggt tttgtccagc tgagaataat ggaaccaaga agcctaccaa
     2281 taagaatggc gatgaagaaa aaggcattac caagcaaccc ccatgttttt aggtcgatcc
     2341 cgaaatgggt gctaagcagg ataggaacac ccgagctgag gcaaatttca attccgacgt
     2401 agataaaaat gcccagcacc attaaaagga caaagcgatt ttttaatagc gaaaagcaac
     2461 tgttgagtgt tgcaggtttc ttatcggact tctcctcctc tatcttcgtg aatcccacgg
     2521 cgaccaacgt taacagcagg gctgacacgt aaataggaaa taaaacttgc caatcggccc
     2581 ccatccattt tgcggcggca aatggaataa tggaacccga cagcgatcca atagctttaa
     2641 tgaactggcc gaagctaagg tttcgggagt acatcccggg ttcagaaaca tcacgcatga
     2701 tgggatttcc tgctacctgc agaattgcag cacctgcgcc cagaagcaga attgtcagga
     2761 ggaacaacgc atagctgccc agtccaaata acggaatggt taacccggct agggcaactg
     2821 ccagacccag catcagcaca actttcttcc cccgtcgatc ttggaatatt cccattggta
     2881 cagacaaaaa gccaaacatg atgaatccca tgaaggcaat tagctgcgct tcaaagtttg
     2941 aaagctcgaa ctgctctttt gccagaccca caaatggtcc aacggcatcg ccaaatccca
     3001 tacataggaa ggccaaaaga attgtaaatg tttttctatt cataactcaa ggtttaaact
     3061 gaaaaagttt tttaggtttt ttaattacaa gcacgctagc ccacagcttt tcgttatcgg
     3121 ggtagagcaa tggaaccccc atattttgac tttgccgaac ttgcaagtaa cgttcgctta
     3181 ttcatgcgtg tttgtgcagt ctgccttaca tgagtgttta aaattactga tttagcacac
     3241 cttaatatga aatcggctgg tgatttgtac tgcgacattg ccaaatgcga tttatgaaaa
     3301 agcaatgaaa cacctgatat aaagtgaatt atagtcattt tatgggctgg ttgtatcatg
     3361 gtttaagttg gtttacaatc ataggtttgt gtcctttgca ttaaggtgac cgccatccat
     3421 tattatcaga aatttgagtt gacgattccc gcaccaaaag ctcaacgggc atcatttcta
     3481 catggccggg ttttgtttcc ccgttatttg taatttgccc tagcaacaac gaaaatgcta
     3541 cgctgcacag ctcggcaaca ggttgcctta tagaggtgat ggatggtttg gagaaatcaa
     3601 agtaaggaag ttcgtcaaaa caaaccagcg caatatcctt aggaataaca atgttgtgaa
     3661 catgacaggc tcggagagtc gctgttgcca ggttattgtt aacggcaaaa actgcatcgg
     3721 gcatattctt atcatggaga cgagtcgtga aaaaatcctc aaccatgtcg accaggcaga
     3781 ggaagggaat attgtgcact tcagtatgaa tggctgcatt ctcaaaaaca cttacgaaac
     3841 cctcaacccg ttcggcaacg gatgaaatgt agcttggggt tagggagagt accactgggt
     3901 tgcgaaaacc ctgatttagc aggtgctgtg ccagtaattc tccaccaacc ttgttgttaa
     3961 caacagccga cggtataggt acagtaggaa agtacctgtc aatgagtacc agcgggaatc
     4021 cggattttac caaagtttct agctcggttg ggttttgctg cgaagaggag agaataattc
     4081 catccacctg ccggttacga aaaatttcaa ggagtttaag ctctttgccc acgctttcgt
     4141 cggtgctgcc tattatcatg ttgtagccaa gctcccaggc caaatcctcc agttgtcttg
     4201 ccaaagaggc ataaaagttg ttggagatat ccgaaacaat taggcccaaa gtgttgctca
     4261 tcccggtacg caggcttcgt gctgtttggt tgggattgta tccatacgcc ctaactgctg
     4321 ccataacttt gtcgcaggtt tgtccagaaa ttccgtaacc cctagccttc ccgtttacca
     4381 caagggatac cagagttttc gatacgccaa ccttttttgc aatgtcgtta agggttacct
     4441 tcttcaccgt gtttgagcct tttccacaaa tatactgtaa atatctaaaa cgatttagcc
     4501 gccaatggaa aatttaatgg tgtaggatca aagttttatt gcagctctga ttgctgccga
     4561 ttttttagtt tggcattgtt cctgaggagc attctacaag taggttttgt ttagaatagc
     4621 ttttcctggc ttggaatggc agtaaagcag caatctgcag tagaagattt attctgagtt
     4681 aggatgtaaa aatttatggt gaatatttac atcatatagg taaaaaggca taaatttatt
     4741 gaaaaatctg attatgagga aattaatttc agccatactc gctattctac ctatgtttac
     4801 gacttttggc caaaggtcgg acattgatag cctgctaaaa cagctgaatg aagcaccgaa
     4861 cacatcggta aaggccgaaa cagccataga tttggctcaa agatatataa acatcgatac
     4921 tgactcagct atggcgttca accaaattgc ccaccagcta gccacggatc tttccgacac
     4981 agccctaatg attcggtgcg aaaacactta tggctttatt tttaacgtga actcaagttt
     5041 tgaaaaggca agggagcgct ttagccgggt gcttgagatg gccataagcg ctcacgattc
     5101 aagccttatt gcctctgcct atggaaatat tggcaacacc tacatgttcg agcaaaaata
     5161 cagcatggcg gttgaaagtt tccttaaggc gctggatatc ttcgaaaaac gaaacaacca
     5221 aaagggagtt gcaaccgttt acggcgtgct gggaaacctg tacttaagcc ttgataagct
     5281 ggaccagtcg ttggagtact accaaaaggc gcaaagcatg ttccatacac tgggatgggc
     5341 cgatt
//
LOCUS       DAOOQX010000044         5310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_18633_length_5310_cov_3.176403, whole genome shotgun sequence.
ACCESSION   DAOOQX010000044 DAOOQX010000000
VERSION     DAOOQX010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18633_length_5310_cov_3.176403"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLX53_01330"
     CDS             complement(<1..321)
                     /locus_tag="PLX53_01330"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOY71753.1"
                     /translation="MMKRILISGFACAMLLIGIVDAQSQVARIYFKDSNHDYGVIKED
                     DGVAYYEFQFSNTGSTPLIIQRVETSCGCTTPDWPKEPIAPGKNGRIKVGYNPSGRPG
                     AFSKT"
     gene            782..1975
                     /locus_tag="PLX53_01335"
     CDS             782..1975
                     /locus_tag="PLX53_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOY71754.1"
                     /translation="MMASLNDKLKELYKTTPVVAGDIYRHIHQNPELSFRESGTSSYV
                     ASFLESLGIGIIKLDKSYSLLATIVGTGEGKTIGLRAELDALPIDEKTGESYASRTAG
                     VMHACGHDFHLASVLGAAIILNNLREFWSGKVVLVFESGEEELPGGARAILESDEFQE
                     VKPDYMVGMHVLPELTIGKVGFCPGRYMASGDEVYLTARGRGGHAALPHTLVDPIVIA
                     STIILSLQTLVSRKAPSNIPSVLSFGRVTANGSTNLIPDEVSISGTFRTMNEEWRAKA
                     HDLIVQMASDIARSTGGECSVEIRKGYPSVYNDPILTQTAVEVASSIFGSENVAPLEP
                     RMTTDDFAYFSQAIPSVFFRIGVGTPGAPTLQLHRPDFRIDEQAFQTSLPMLTGLCLK
                     LLTSS"
     gene            complement(2082..3848)
                     /gene="sppA"
                     /locus_tag="PLX53_01340"
     CDS             complement(2082..3848)
                     /gene="sppA"
                     /locus_tag="PLX53_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOY71755.1"
                     /translation="MKSFFKYLLATIVGVLLSFLLIFLIMMGIAGSMVSSADKAVEVK
                     DNSILYMDLKNEIVDRASSNPFETLDLATFEPQKNLGLNEILKAIAKAKDDPRIKGIL
                     LEFDVVNAGAATTGEIREALREFKESGKFVVSYSDTYSQKAYYLASVADSIYLNPEGM
                     VEWLGLRSEIMFYKKALEKLDVEPQIIRHGKFKSAVEPFMSERMSPENREQTLTYLSS
                     IWGSWVDEISKSRNIAVAELNRLADNMEVYDGKSCMKSKLVDSLIYKDQLLEKLKAFT
                     GIPENRNLNTVTLEKYFKVPPVKKTAFSKNKIAVIYAQGEIGMGDGSNTAIGSEGLSK
                     AIRQARRDSTIKAIVFRINSPGGSALASEVIWREVELAGQAKPLVVSMGDVAASGGYY
                     IASPATTILASPTTITGSIGVFGLFFNLQKTLDKKLGINVDVVKTNAHSDFFSMFRPM
                     TAEEKAVGQRFVEQTYQTFIGHVSQGRGIPVERVDEIGQGRVWSGANAKEIKLVDDFG
                     GLTAAIKLAAEKANLDTYRLVELPKQKSPFEELLKGFTTEAKAMLVKDDLGKGYKYYN
                     RVMQMVENQGVQARIPYDVEVY"
     gene            complement(4254..4859)
                     /locus_tag="PLX53_01345"
     CDS             complement(4254..4859)
                     /locus_tag="PLX53_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOY71756.1"
                     /translation="MNEKGIIKFRCSWVNADPLPYEAVADLNRWRCILYNKGLVGVDC
                     NGVGFGNLSKRYLHNQFVITGSGTGQLTELTTMHYTLVTDFSIGRNWVEAKGPVIASS
                     ESLTHAALYQCDKAIKAVFHIHNLELWKRIIGKVPTTLSNAEYGTPQMAMEIKRLYRQ
                     TDLRKTRVLVMAGHQEGIMAFGLDADEAGKRILSLMKEYNP"
     gene            complement(4856..>5310)
                     /locus_tag="PLX53_01350"
     CDS             complement(4856..>5310)
                     /locus_tag="PLX53_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOY71757.1"
                     /translation="GITHTVIADNTGGHLMQHGLVDLVLTGADRITTNGDAANKIGTY
                     LKALAAHDNGVPFYIAAPSSTFDWSLSDGLSQIPIEQRHPDEVRYVGGWLDGRLQNVL
                     VCPDESPAANFAFDVTPARLISGIITERGICKPLPAGIKKVFPEKFNY"
BASE COUNT         1434 a         1284 c         1188 g         1404 t
ORIGIN      
        1 ggttttggaa aaggcccccg gacgccccga tgggttatat cccaccttaa ttcttccatt
       61 tttacctggg gcaatgggtt cttttggcca atcgggtgtt gtgcagccgc acgaggtttc
      121 aacccgctga attattagcg gtgtacttcc tgtgttggaa aactgaaact cgtagtatgc
      181 aacgccatcg tcctccttta tcactccgta atcgtgattg gaatctttaa agtaaatcct
      241 agccacctgc gactgagcat caacaatacc tattagcagc attgcacagg caaaaccgga
      301 aatcaaaatc cttttcatca ttatctacat ttagtatgtt gtaacttgac tgcctcaaaa
      361 cctctcaaat tcaaaacaaa ccatagttat gctttaggct tatccatgta tacctggtaa
      421 gcagaactca cacaggttca agtccttcaa aacgaaaacc aggtaaaaat acttcagcaa
      481 tatcaatact caatattttc aaaagctaaa aaatactgca agcaaaacga ttttcacctg
      541 gcaggtagta aaccaaatca acatcaaagt tactaattac aggcaactac aacaattttt
      601 tcaaagtaaa aaagtgcgag cctcaaacag taaaccaaat tttgactaaa aggctttgag
      661 ggaaataatc taaacatctg gctttagtca gtaatgtaag gttttcgtcc gtaaaagtgc
      721 tgatgatgaa tattttttcg gaaattacat gtaagtttga atggtttaac caggttgcaa
      781 aatgatggcc agcctaaatg acaagttaaa ggaactctac aagactactc cggttgttgc
      841 tggtgatatt taccggcata tccaccaaaa tcctgaacta tcgttccgcg aatcaggcac
      901 atcaagctat gttgccagct ttttggaatc gttaggcata gggataataa aactagataa
      961 gagctacagc cttttggcaa ctattgtcgg cacaggtgaa ggcaaaacca ttggactcag
     1021 ggctgaactg gatgcactgc ccattgatga gaaaaccgga gaatcgtacg cctctcgaac
     1081 cgcaggagta atgcacgcct gtgggcacga ttttcacctt gcgtcggttc taggtgccgc
     1141 aataatcctc aacaacctac gcgaattctg gagtggaaaa gtggtgctgg ttttcgaatc
     1201 gggagaggag gaacttcccg gtggtgccag ggccattctc gaatcggatg aatttcagga
     1261 ggtaaaaccc gactatatgg ttggcatgca cgtgctaccc gaacttacca tcgggaaggt
     1321 tggcttttgc ccaggacgct acatggcatc gggcgatgag gtttatctaa cggctagagg
     1381 acgaggggga cacgccgcat taccccacac cctggttgat ccgatagtca ttgcctcgac
     1441 gattatttta tcccttcaga cactggtaag ccgcaaggca ccttccaaca tcccatccgt
     1501 tttgtcgttt ggccgggtaa cggcaaatgg gtcaactaat cttataccag acgaggtaag
     1561 catttccgga actttcagaa caatgaacga ggaatggagg gcaaaggcac acgatttaat
     1621 tgtccaaatg gcaagtgata ttgccagatc tacgggagga gaatgctctg tggaaattcg
     1681 caagggctac ccgtcggttt acaacgaccc aatcctcact caaacagccg ttgaggttgc
     1741 aagcagtatt ttcggttccg aaaacgttgc tcccctggag ccaaggatga ccaccgacga
     1801 ttttgcctac ttcagccagg caatcccatc ggtgtttttc agaattggcg ttggcacgcc
     1861 aggcgctcca accctgcagc tgcatcgtcc ggatttcagg attgacgagc aagcgttcca
     1921 aacgtcgtta cccatgctaa ccgggttgtg ccttaaactt ctaacatcca gctaacaaac
     1981 ttctgttggt agctatgctg ttgaaagcca aacggctcag ggtaaaccct aagccgctca
     2041 acacttttca tgcctatttt ccgtgcatga atggcaacat tctagtaaac ttcaacatca
     2101 taggggattc tagcctgcac tccctggttt tcaaccatct gcataaccct gttgtagtac
     2161 ttatatccct tgcccaaatc gtccttcacc agcatggctt tagcctcggt tgtaaacccc
     2221 ttaagtagct cctcgaaggg cgatttctgc ttaggcagct ccaccaaccg gtatgtgtcg
     2281 aggtttgcct tttcggcggc cagctttatg gctgcagtta gcccgccaaa atcatctacc
     2341 aacttaattt cttttgcatt tgcaccgctc cacacgcgac cctggccgat ctcgtcaact
     2401 ctttccacgg gaatgcctct tccctgagaa acatgcccga taaaggtctg gtaggtttgt
     2461 tcaacaaaac gctgcccaac ggccttttcc tcggcagtca tggggcgaaa catggagaaa
     2521 aagtcggaat gggcgttggt tttcaccaca tccacgttaa tacccaactt tttatctaaa
     2581 gttttttgaa ggttgaagaa taggccgaag accccaatag aacccgttat ggtggttgga
     2641 ctggcaagaa tggtggttgc aggtgaagca atatagtacc cacccgaagc agccacatcg
     2701 cccattgata ccacaagagg tttagcctgt ccggccagct caacttctcg ccatatcacc
     2761 tcggaggcaa gagcactacc accaggagag ttgatgcgga aaacaatggc cttgatagtg
     2821 ctatcgcgcc tagcctgacg aatggccttc gaaagacctt ccgagccaat tgccgtattg
     2881 ctaccatcac ccatgccaat ctctccctgg gcataaataa cagctatttt gtttttggag
     2941 aaagccgttt ttttaacggg aggcaccttg aagtatttct ccaaggtaac ggtattcagg
     3001 ttacgatttt cgggtattcc tgtaaaagcc ttaagcttct ctaacagctg atccttgtat
     3061 atcaggctgt caaccagctt cgacttcatg caggatttac catcgtacac ctccatgttg
     3121 tcggccaggc ggttaagctc tgcaacagct atgttacggg attttgaaat ttcatcaacc
     3181 caactgcccc aaatagagga aaggtaggta agagtttgct cgcgattctc ggggctcatc
     3241 ctttcactca tgaatggctc aacagcgctt ttaaacttgc catgacggat aatctgtggt
     3301 tcaacatcca gcttttcaag cgcttttttg tagaacataa tttcggaacg caggccgagc
     3361 cactccacca tcccctcggg gttaaggtag atgctgtcgg ctacagaggc caggtagtat
     3421 gctttttggc tataggtatc actatagcta acaacaaact tacccgactc cttaaattcc
     3481 ctcagggcct cacggatttc gccggttgtt gcagcaccag cattgaccac gtcaaactcc
     3541 agcaaaattc ccttaattct tgggtcatcc tttgccttgg caattgcttt tagtatctca
     3601 ttaaggccaa ggtttttctg gggttcgaaa gttgccaggt cgagtgtttc aaaggggttg
     3661 ctgcttgccc tatcaacaat ctcgtttttt aaatccatgt aaagaataga gttgtccttt
     3721 acctcaacag ctttatcggc tgatgaaacc atgctgccgg caatacccat cattatcaaa
     3781 aatatcagaa gaaacgacag cagcacgcca actatggtgg caagcaagta tttaaaaaaa
     3841 cttttcatga tacaggtttt ttagatgatt actactaaaa gttgaatttt cagatttgct
     3901 ctgcaaacag gcattccacc ttttaaaact caaacttata aaaagttatc aaaacaagca
     3961 taaaatatga agcaaaaaaa caaaagtgta gctgtagtgg gtttgtcaat gttgccgagc
     4021 tcgtgcggaa aaatcatctg ttgtttgaac taatcagcca ggcgtaaagc ctgtgtatgc
     4081 ctactcagca aatttttagt taatggaaaa attaaccaat cgcccctgag ctaaagtttg
     4141 gggtagctgc tatgcgacaa ctgaacttca ttgttttagt aggacggaaa gaatggaagg
     4201 taggtagtaa agatttatcc gcgaagttta tagcactgcc tcaaaaactt ggcttatggg
     4261 ttgtattcct tcatgagcga taaaatacgc ttccctgctt catcggcatc aaggccaaag
     4321 gccataattc cctcctggtg accagccatg accagcactc tggttttacg gagatcggtt
     4381 tgtctgtata gccttttgat ttccatggcc atttggggag tcccgtactc ggcattagac
     4441 agcgttgtgg gcactttccc gataatccgc ttccaaagtt caaggttgtg aatgtggaac
     4501 accgccttaa ttgccttatc gcactggtaa agtgcggcat gggtaagcga ttcggaagaa
     4561 gcaataaccg ggcctttggc ctccacccag ttacgcccaa tgctgaaatc tgtaaccagc
     4621 gtgtagtgca tggtggtaag ctcggtaagt tgtcctgttc ctgagcctgt aatgacgaac
     4681 tggttgtgaa ggtaacgctt gctcaggttt ccaaaaccaa ccccgttaca atccactcca
     4741 accagacctt tgttgtagag tatgcaacgc caacggttca agtcggcaac cgcttcgtag
     4801 ggaagagggt cggcgttaac ccagctgcac ctgaatttaa taatgccctt ctcgttcaat
     4861 agttgaattt ttcaggaaaa actttcttta tgccagcagg taagggcttg caaattcccc
     4921 gttcggtaat aatcccggaa attaatcggg cgggtgtaac atcgaaggca aagttggcgg
     4981 cagggctttc atcagggcaa accagcacgt tttgaagcct gccatccagc cagccaccaa
     5041 cgtaccgcac ctcatcggga tggcgctgct cgatgggtat ctgcgaaagc ccgtcgctga
     5101 gcgaccagtc gaaggtggat gaaggcgctg caatgtaaaa tggaaccccg ttgtcgtggg
     5161 cagcaagagc tttaaggtag gtaccaattt tgttggcagc atctccgttg gtggtgatac
     5221 ggtcagcacc ggttaacacc aaatctacca acccatgctg catgaggtgt cctccagtgt
     5281 tatcggctat caccgtgtgg gtaattccct
//
LOCUS       DAOOQX010000045         5309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_18640_length_5309_cov_3.788352, whole genome shotgun sequence.
ACCESSION   DAOOQX010000045 DAOOQX010000000
VERSION     DAOOQX010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18640_length_5309_cov_3.788352"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLX53_01355"
     CDS             <1..191
                     /locus_tag="PLX53_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71758.1"
                     /translation="QAKFHSLKPLYILLRVGIPPGVKKFSPLKPGTGTSALDSFVENG
                     GLPHPDQFSQVQRKFFYL"
     gene            242..2740
                     /locus_tag="PLX53_01360"
     CDS             242..2740
                     /locus_tag="PLX53_01360"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY71759.1"
                     /translation="MIKPKVLTGFIIILLFSASSIIVTYQGFRELNLTRQEIGQPGQK
                     LAVINRLISGVYGGEADVRMFIITNDTGYLESFEERKKKVTSVLNSLDGLMAETPGQQ
                     QNVKQIKALLSRKWAIVNELLALRKTYTDDRFYEEALKQIERAGTESRIRASVAKTTT
                     VTTTLHDTVVTLENKDENLFGKIKRFFVGPERVDTVSRSVNIETRLDTIDKGQYVSDS
                     IINNLVKLINGIRADQRKYQFDLGVKEAELLEQDRELMKKIESIVSSLERQELSSSIR
                     QSVLAQDLVNRSIIKILALGSFTFILLLVLLAVIFRDISRSNQYRTQLLEAKQYAERL
                     LRVKEQFLANISHEIRTPLSAVIGLTHQLGKTDLNARQQDYVSTLSSSADHLLSVIND
                     ILDYSKLESGQVRFETAMFDPVNVLESVTQTFNPKAEEKQLLVHTNIDPAVPHVLWGD
                     SFRLKQALMNLLSNAIKFTESGSIIVSLGVVRLTPEYVKLQFTVADTGVGIPPEQQQA
                     IFEEFTQADSSISRKYGGTGLGLTLVKKIVELQGGEISLESKINEGTIVKFSIPYALT
                     GVETKEETLERSSFAIPNNTAILVIDDDEVNLLIVTEMATTLGLQVDTLSKPEALYET
                     LSSKKYSAILTDIQMPGLNGYEVVKLVEQFNENIPVVAITANSMVDSPEHFTSHGFAG
                     YLIKPFVETDLQKVLSPLIGIPKPVKQLQVSTKSRRSSPAQIDLSDLYRFTGGDKNSL
                     RLILTSFLDNTYRNADELNRYIREKNLKQASEVAHRMKSAFSQLKIYHISSLLQKIET
                     LKPDKYRAANIYLDYLNKQIKPVVKEIRELLEKL"
     gene            complement(2786..>5309)
                     /locus_tag="PLX53_01365"
     CDS             complement(2786..>5309)
                     /locus_tag="PLX53_01365"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOY71760.1"
                     /translation="IPYHAIKLHNGNIGVGTVQSDWLSFFELNADSSSPSTIVNPQTG
                     LQAAQITYLYQNGESPVWATLFDGGISRIELHSPIRRFSTESGINELILDIIQFNGTL
                     YVATINGVYYQTAAANGMSVFKPVEGINGVVWSLLKFTPPGGSPMLLAGSYVDGVFEI
                     KGNRAISISKPLYRSFPNVQHQCLTLYQSVLHPNMLYVGGISRLTYIEWSKGEWVKTQ
                     NIALDEIRSEVWSIGEDARGNLWLGTNLKGVYMIDPHEKVYNFNSSSDFSGFKLLRIY
                     PDGDSLYVLTPKGIFYYSYSDSTFRKCGLLGKNSLENGFSRLLKVGNEIVAACYNDST
                     GKYWVCTMAKNLSGEWVTNDKPFKRLPNKGADALYVDDGGVLWIGIGKELFSFNTRVK
                     RSYNAPYHALVRSVYAKDSLLFNGSFSEVDQDGRKITLLQQPQSKIPQLKYRYNGLVF
                     SFSSPYFEEEAELLYSHYLEGSDETTWSAWDGRTEVTYTNLSEGKYTFHVKAVNIFGE
                     ESIPATFTFVIHPPWYRTLLAYFCYFIFFIGLVWGLVRLNTRRLIAEKERLEKIVEER
                     TAEVVAQKEEIERQKEKISIQNEEITSSIQYASRIQSALLTPSELVSRIFPEHFILYL
                     PRDIVSGDFYYVTQVESKKICIVADCTGHGVPGGFMSMLGISFITQILSEVKELQAAD
                     ILNRLRQLVISTLHQTSDVGGSKDGMDITIYIVDEQSMTMQFAGANNPLILIRNGEVT
                     QVKGDKMPIGIHLRGEVPFTNNVIEIQKGDVMYTFSDGYVDQFGGELGRKFMIKDFRE
                     LLLKIHTYPMAEQRQILLDTLISWQGDTPRIDDVVVMGVRIV"
BASE COUNT         1466 a         1309 c         1225 g         1309 t
ORIGIN      
        1 gccaggctaa atttcattct ctaaaacctt tatacatcct gttacgtgtg ggtattccac
       61 caggtgtaaa gaaattttca cctcttaaac caggcacagg cacgagtgct cttgatagct
      121 ttgtagaaaa tggagggcta ccccacccgg accaattcag ccaggtgcaa cgtaaatttt
      181 tctacctttg aagtgttgtt aaactgttat ggccgattcg cttgacaaac caaagggctt
      241 catgataaaa ccaaaagtgc taacgggttt tatcatcatt ctgctattct cggcctcatc
      301 cattatagta acttaccagg gtttcaggga gctgaacctt acccggcagg agattggaca
      361 acccggccaa aaactagccg tcatcaaccg gctgataagc ggggtgtacg gaggtgaagc
      421 cgacgtaaga atgttcatta ttaccaacga cacgggctac ctcgaaagtt ttgaggaaag
      481 aaagaaaaag gttacatcgg tgctcaactc gctggatggc ttaatggctg aaacgccagg
      541 gcagcaacag aatgttaagc aaataaaagc cttactttca cggaaatggg caattgtaaa
      601 tgagctcctt gcactcagaa aaacctatac tgacgatagg ttttacgagg aggcgctaaa
      661 acagattgaa agagccggaa ccgaaagccg tataagggca tctgtagcca aaacaaccac
      721 cgtaaccacc accctgcacg acacggtggt aaccctcgag aacaaggatg aaaacctttt
      781 tggaaaaata aagcgatttt tcgttggtcc cgagagggtt gacacggttt cgcgaagcgt
      841 taatatcgaa acccggctcg atactattga taaggggcag tacgtttccg attcgataat
      901 caacaacctg gttaagctca ttaatggcat cagagccgac cagcgtaagt accaattcga
      961 cctgggcgta aaggaagccg agctgctgga gcaggacagg gagctgatga aaaagataga
     1021 gagcattgtt tcatcactcg aaaggcagga gctttcttcg tccatacgcc aatcggtgct
     1081 tgcccaggac ctggtaaacc gctcaattat aaagattctg gcgctaggct ccttcacctt
     1141 tatcctgctg cttgtcctgc tggctgttat attcagggat atttcgcgaa gcaaccagta
     1201 ccgtacccag ctgctggaag ctaagcagta tgccgaacgt ctcctgagag taaaggagca
     1261 atttttggct aacatcagcc acgaaatacg aacgcccctt agtgcggtta ttggtctaac
     1321 gcaccagctc gggaaaaccg acttgaatgc caggcagcag gactacgtca gcacgctcag
     1381 tagctcagcc gatcacctgc tgtcggtaat caacgacatt ttggactact ccaagctcga
     1441 gtcagggcag gtgcgatttg aaacagcaat gttcgacccg gtgaatgtac tggaaagcgt
     1501 tactcagacc tttaatccga aagctgaaga aaagcagctg ctcgttcaca ccaacattga
     1561 ccctgccgtt ccgcatgttt tatggggtga ttcgtttcgc ctgaaacagg cgcttatgaa
     1621 cctgcttagc aatgccatta agttcaccga aagtggaagt attatagtca gccttggcgt
     1681 ggtaaggcta actcccgagt atgttaagct gcagttcaca gtggccgaca cgggagttgg
     1741 gattccccct gaacagcagc aggctatttt tgaggagttc acccaggccg acagcagcat
     1801 ttccaggaaa tacggaggaa caggacttgg gctcacgctg gtgaaaaaaa ttgttgagct
     1861 tcaggggggt gaaatttcgt tggaaagcaa aatcaacgaa ggaacaattg ttaagttctc
     1921 cattccctac gccttaacag gggttgaaac gaaggaggag actttagaaa gaagcagctt
     1981 tgccatacca aacaatacgg ctattctggt aattgacgat gacgaggtta acctgctaat
     2041 tgtaaccgaa atggctacta ctctcggatt gcaggttgat acgctgtcta agcccgaagc
     2101 actctacgaa actcttagca gcaagaagta tagcgccatt cttaccgaca tccagatgcc
     2161 ggggcttaac ggctacgagg tggtgaagct ggttgaacag ttcaacgaaa atattcctgt
     2221 tgtggccatc accgccaata gcatggttga cagcccggag cacttcacca gtcatgggtt
     2281 tgccgggtac ctgataaaac cctttgtgga aaccgacctg caaaaggttc tgtcgcccct
     2341 gattggcatt ccaaaaccgg tgaagcagct gcaggttagc acaaaaagca ggcgtagctc
     2401 tccggcacag attgatctgt cagatttata ccgatttacc ggcggtgata agaactccct
     2461 caggttgata ctaacctcct tcctggacaa cacctaccgt aatgccgacg agctgaaccg
     2521 gtatattcgt gaaaaaaatt taaagcaggc ctcggaagtt gcccatcgaa tgaagtcggc
     2581 atttagccag ctcaaaatct atcacatttc gtccctgctc caaaaaatag aaacgctcaa
     2641 gcccgacaag tacagggctg ccaacatcta cctcgactac cttaacaagc aaattaaacc
     2701 ggtggtaaaa gaaattcgtg agctgctgga gaagttgtag ccggggtaaa cccgaagttt
     2761 tcccaaacat cggattttaa tcaggtcaaa caatccttac acccattacc acaacatcgt
     2821 caatccgtgg agtatcgccc tgccacgaaa taagcgtgtc gagcagaatt tgcctttgct
     2881 cggccattgg gtaggtgtga attttcagaa gcagctccct gaagtcctta atcatgaatt
     2941 ttcgccccaa ctcaccgcca aactggtcaa catagccatc ggagaaggtg tacatcacat
     3001 cgcccttctg aatttcgatg acgttgttgg taaacggcac ttcaccacga agatgaattc
     3061 caatgggcat tttgtcgccc tttacctggg taacctctcc atttcttatc aggataagcg
     3121 ggttatttgc ccccgcaaac tgcatggtca tggattgctc atcaacaatg tagatggtga
     3181 tgtccatgcc gtccttgcta ccgccaacat cgctggtctg gtgcagcgtg cttataacca
     3241 gctgccgcag cctattcagg atgtcggctg cctgtagctc cttaacctcg cttaatattt
     3301 gggtaataaa ggagataccc agcatactca taaatccacc cggtacgcca tgacccgtgc
     3361 agtcggcaac tatacatatt tttttgcttt ccacctgggt tacgtagtaa aaatcgccac
     3421 tgacaatatc cctgggtaag tatagaatga agtgctcggg aaatatcctg ctgaccagct
     3481 cggatggtgt aagcagtgcg ctttgtatcc ggctggcata ctgaatactg ctggttatct
     3541 cttcattctg aattgaaatt ttctcctttt gcctttcaat ttcctccttt tgggccacaa
     3601 cctcagcagt gcgctcctca acaatctttt caagtcgttc cttttcagca atcagccttc
     3661 gggtgttcaa ccttaccaac ccccaaacca gccctataaa aaagataaag tagcaaaaat
     3721 atgccagaag ggttctgtac catggtggat ggatgacgaa ggtgaaggta gccggaatac
     3781 tttcctcacc aaaaatattg acagctttta catgaaaggt atatttccct tcactcaggt
     3841 tggtataggt aacctcggtt ctcccatccc atgcgctcca ggtagtttca tccgaaccct
     3901 ccaggtagtg gctgtagagc agctcggcct cctcctcaaa atagggcgaa gaaaagctaa
     3961 aaactaagcc attgtaccgg tactttagct ggggaatctt gctttgaggt tgctgcagaa
     4021 gggtaatctt tctaccatcc tggtccacct cggaaaaaga gccgttgaaa aggagggaat
     4081 ccttggcata aacgcttcga accaaagcat ggtatggagc gttgtagctc cgcttaaccc
     4141 tggtattgaa gctgaacagc tccttcccta tcccaatcca tagcaccccg ccatcatcaa
     4201 catacagagc atcggctcct ttgttgggaa gccttttaaa aggcttgtca ttggtaaccc
     4261 attcgccgga taaattcttg gccatcgtgc acacccagta cttgccagta gaatcgttgt
     4321 aacaggcggc aacaatttca ttccccacct tcaacaaacg tgaaaagccg ttttccaaag
     4381 aatttttacc caacaatcca catttcctga aggtgctatc gctgtagcta tagtaaaata
     4441 tgccctttgg agtaagaacg taaaggctgt cgccatcggg gtaaatacgt agcagcttaa
     4501 aaccggagaa atcgctgctg gagttaaagt tgtaaacttt ttcgtgggga tcaatcatgt
     4561 aaacgccctt aaggtttgtc cccagccaaa gatttcccct tgcgtcctcg ccaattgacc
     4621 atacttcgga gcgaatctca tcaagggcta tgttctgggt tttcacccat tcccccttgc
     4681 tccattcaat gtaggtaagc ctcgaaatac ccccaacgta tagcatgtta ggatgaagta
     4741 cggactggta aagggtaaga cactggtgct gcacgtttgg aaaagaacgg taaaggggtt
     4801 tgcttatcga aatagccctg ttgcccttaa tttcaaaaac cccatcaaca tacgaaccgg
     4861 cgagtagcat tggcgagcct cccggaggag tgaactttaa caatgaccac acaacgccat
     4921 tgataccctc aaccggctta aataccgaca ttccgttagc agccgccgtt tgatagtaaa
     4981 ccccgttgat ggttgcaacg taaagcgtgc cattaaactg aataatatcc agaattagct
     5041 cgttgattcc gctttcggtc gaaaaccgtc ggatgggcga atggagctct attcgggaaa
     5101 ttcctccgtc gaacagtgtg gcccacaccg gcgattcgcc attctggtaa agataggtaa
     5161 tttgagcggc ctgaagccct gtttgtgggt ttactatggt gctgggcgag gaggaatcgg
     5221 cgtttagctc aaagaagctc agccagtcgg actgcaccgt tccaacccca atattcccgt
     5281 tatgcagctt gatggcatga tagggaatt
//
LOCUS       DAOOQX010000046         5256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_18924_length_5256_cov_2.750817, whole genome shotgun sequence.
ACCESSION   DAOOQX010000046 DAOOQX010000000
VERSION     DAOOQX010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18924_length_5256_cov_2.750817"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2017)
                     /locus_tag="PLX53_01370"
     CDS             complement(<1..2017)
                     /locus_tag="PLX53_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71761.1"
                     /translation="MNAPLSNNPQGINIRIVGLLGLLFFLSAEGMAQYAPAGIGTSSE
                     NVLWLRSDQGTSTTTNGEPVSGWNDISGNANHAAQGTAGNQPIYISSGINGLPTIRFD
                     GTNDHLTVADADNLDNTNGVSVFVIAQPVSPDALARGLVSKRVSAGNEEAFYLFTYTG
                     RNLYFNASTYRINGNDPVTDQPQIFSAIYDGSVANPRSKIFYNGEESGNGNGPVSIAN
                     MASDLHIGILNPGYAQAFNGDISEIIVYRDNLNKAQRLIVEAYLANRYNIAITPTSYS
                     STTHTQDFIGIGHSGGDRYSQTRNVGSGLLLSERNETLDETNEFVFAGHDGTPHGFNT
                     NDLPTIPGVNLDQRWDRIYYVERVQNGVIDAGSTDVRITFDFTEAGLSLSTDRVYYLL
                     YRSGTSGTFSSVPGGTGVASNGKVSINLSSSNFASGYYTLARSDQQVKTWYSLNDGNW
                     NDYETWSLKPNEPDNPSHEIPGIMDRAVIQNNKTITVTTDDTQCGILDVIDGIVDFGT
                     TTGSTFSTITGQVNGKIRLAADNFPSGDAGEFADASSGGTVEYYGTGYNLSTSRTFRN
                     MVVNLTNTTNKLYLLSNYTLNGSLTITRGEFYFGDNVSTTSRSLTVYGDFVVEANGKV
                     HTGSANARHQLNLYGDFTNRGNIQLTNRAAADYTAEATDGIVDANFLS"
     gene            complement(2543..3925)
                     /gene="hydA"
                     /locus_tag="PLX53_01375"
     CDS             complement(2543..3925)
                     /gene="hydA"
                     /locus_tag="PLX53_01375"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HOY71762.1"
                     /translation="MAEKLEYIIKNGTVVTSTNTQKVDILIKNGKIAALANEFNDDMF
                     QTINANGMYILPGGVDPHVHLHLPTPAGYSSDDFHTGSIAALYGGTTTIIDFVTPHRG
                     QPLAEALAARIEEANECLTDYSFHVSPVDWRRESEDEIKRCIDLGFPSFKIYMAYLDT
                     NGLHDTDIQRVMGVVGRNGGMVTAHCELGEEVDRLRYSFYRQGKTQPIYHQLSRPPQV
                     EVKAVERAIAMAQQANCSLYVVHLSAGSSLEPVHRARAAGQKVYAETCPQYLLLNSSC
                     YTDDFWPAAKYVISPPLRSPSDQEQLWKGIQDEDIQTVGTDHCPFSSSQKAYGINDFR
                     KIPNGAGGIEHRLALLYTFGVLEGNISINRMVDLYSTQPAKIFGLYPQKGEIAVGSDA
                     DLVIWNPAPTSTIQASNHHSQADFNIYEGMKVKGGANFVICHGRVAFANGIEQPELPK
                     GNLLRRKAQG"
     gene            complement(3918..4556)
                     /locus_tag="PLX53_01380"
     CDS             complement(3918..4556)
                     /locus_tag="PLX53_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71763.1"
                     /translation="MNADILEWIGYTASVIIALSMALNSIVKFRWVNLTGALLFSIYG
                     FLIGALPVGILNGIIVVTDLYYLIRIYSQKDTFEILEIRPDNKYLLRFLDYHSKDIQK
                     YFPNFSYSPDENTISFFVLRNMVVAGVFVARKVADRTLEVQLDYVLPEYRDFKNGRFV
                     YSWLVQNFTEHGFARVWAKAETEKHRNYLLKVGFNITSDGHFYKTLNENQHG"
     gene            complement(4612..>5256)
                     /locus_tag="PLX53_01385"
     CDS             complement(4612..>5256)
                     /locus_tag="PLX53_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOY71764.1"
                     /translation="IHVAEGIYDQEHCHQNYGQSVIERLNSFGLLSSSKSILVHCLHL
                     SGNERDIIASSPCWVVQNTDSNLNNNVGYFNSKGLGNNLMLGTDGMHSDMLQSMKSAY
                     FVGQNFDPMSPMQAYSRLRNAHRYLSSNNFSGDDESNLLVIDYNSPTPVTPDNFAAHL
                     VFGINSSHIRDVISNGKLIVKNHRLTLVDEEALLIESRKIAAYLWKRMGDVGAE"
BASE COUNT         1322 a         1191 c         1271 g         1472 t
ORIGIN      
        1 cgctcaggaa gttggcatca acaatcccgt cggttgcctc ggctgtgtag tctgccgcag
       61 cacggttggt aagttgaatg ttaccacggt tggtaaaatc gccataaagg ttaagctggt
      121 gccgcgcatt tgcggaaccc gtatgaactt tcccattggc ctcaaccaca aaatccccgt
      181 aaacggttag cgagcgggag gttgtggaga cgttatcacc aaaataaaac tcacccctgg
      241 taatggtcaa cgagccgttt agggtgtagt tcgacagcaa gtagagctta ttggtggtgt
      301 tggtcaggtt tacaaccata ttccggaagg ttcgggaggt tgaaaggttg tatcctgtcc
      361 cgtagtactc cacggttccg cccgaggagg catcggcaaa ttcaccggca tcgcccgatg
      421 gaaaattatc ggctgccagc cttatctttc cgtttacctg gccggtaatg gttgagaacg
      481 tactgccggt tgtggtacca aaatctacaa tcccgtcaat cacatcaaga attccgcact
      541 gggtatcatc tgtagtaaca gtaatggttt tattgttctg tataacggct ctatccatga
      601 ttccaggaat ttcatgcgag gggttatcag gctcgttggg ttttaggctc catgtttcgt
      661 aatcgttcca gttcccgtcg tttagcgagt accatgtttt tacctgctgg tcgcttcttg
      721 ccagggtata gtatccgctg gcaaaattag agctgctcag attgatagaa acctttccgt
      781 tggaggcaac cccggttcca ccagggacag aggagaaggt tccggatgtt ccagatcggt
      841 agagcaggta gtaaaccctg tcggtcgaaa gggatagtcc agcctcggta aagtcgaagg
      901 taatccgtac atcggtgctt ccagcatcaa taactccatt ctggacccgc tcaacgtagt
      961 aaatcctatc ccaacgctga tcaaggttaa caccgggaat ggttggtagg tcgttggtat
     1021 tgaagccatg gggtgtgcca tcgtgtccgg caaaaacaaa ctcattggtt tcatcgagcg
     1081 tttcgttgcg ttcggatagg agcaagccgg agccaacatt tctggtttgg ctgtacctat
     1141 cgccacccga atgaccaatc ccaataaaat cttgcgtgtg cgtggttgaa gagtaggagg
     1201 ttggcgttat ggcaatgttg taacgattag ccaggtaagc ctctacaatt aacctttgcg
     1261 ccttattaag gttgtctctg tacacaataa tctccgaaat atcgccgtta aatgcctgag
     1321 catatccggg gttaaggatt ccaatgtgca ggtcggaggc catatttgca atgctaaccg
     1381 gcccgtttcc gttgccgctt tcctcaccat tgtaaaagat ttttgaacgt gggttagcca
     1441 ccgatccatc gtaaatggcc gaaaatatct ggggttggtc tgttacagga tcgttgccgt
     1501 tgattcggta agttgaggcg ttaaaatata ggttcctacc ggtgtacgtg aatagataga
     1561 aggcctcctc atttccagcg cttactcttt tggaaaccag tcctcttgcc aaagcatccg
     1621 gactaacggg ttgggcaata acaaaaacgc ttaccccatt ggtattgtca aggttgtcgg
     1681 catcagcaac ggtcaaatgg tcatttgttc catcaaatcg aattgtaggc aagccgttaa
     1741 ttcccgaaga aatgtaaatg ggctggttgc ccgctgttcc ctgagcggca tggtttgcat
     1801 tcccgctaat atcattccag ccactcactg gttctccgtt tgtggtggtt gaggttccct
     1861 ggtcggagcg cagccacagc acattttcgc tgcttgtgcc tatgcccgcc ggagcatact
     1921 gggccattcc ttcagctgag aggaaaaaca gtaagcctaa aaggcccact atccttatgt
     1981 tgatgccttg tggattattg gacagaggcg cattcatctt gtggctattt tgtagttatc
     2041 ataaaataaa attgcaatat ttactccaaa tttaataaaa ataagtatat cagttagtta
     2101 ttccagaaaa ggaaaaatag cagaccaatt agtgttcaaa aacgggcatt attgtgagct
     2161 gttatgtaaa aatatacaaa ctaaatgtga aaagttaaag ggaagaagat taaagaattg
     2221 acttttgatt aatttccgca ggtgaaggat tttatcttca cttcaaagca acttcatccc
     2281 tgtctttatc ccttttttgt cccagcgctt tggaaaattc cctgcgttgc gatgcaactg
     2341 gtcagggttg cgctttggaa gatttgctac ttgcatcgcc aattcttcac ctaccgcgtc
     2401 ataccccggg cttgcgcctg gggttaatca tgtttcgccc cttctgggct taaaacacat
     2461 cctgaaattg ttctgaatct tggatttttt tccctgagct tgtcgaagtt tgaacctata
     2521 acctttatcc ttgtattaac ctttaacctt gtgcttttct cctcaatagg tttcctttgg
     2581 ggagttccgg ttgctctatc ccgtttgcaa aggcaactcg tccatgacag ataacgaagt
     2641 tagctccgcc tttcaccttc atgccttcat atatgttgaa gtctgcctgc gaatgatggt
     2701 tgcttgcctg aatggttgag gtgggggccg gattccaaat aaccaggtcg gcatcggagc
     2761 caacagctat ttcgcccttt tggggataaa gtccgaatat ttttgccggt tgggtagagt
     2821 agaggtcaac catccggttg atgctaatgt tcccctccaa aacgccaaac gtgtagagca
     2881 aagctaaacg atgttcaatt ccccctgcgc cattgggtat ttttcggaaa tcgtttattc
     2941 cataggcctt ttgactagaa ctgaaggggc aatggtcggt tcccacggtc tggatgtctt
     3001 catcctgaat gcctttccaa agctgttcct gatcgctcgg tgagcggagc ggcgggctaa
     3061 taacatactt tgcagctggc caaaaatcgt cggtgtagca ggagctgttg agcagaaggt
     3121 actgcgggca ggtttcggca taaaccttct gtccggcagc tcttgcacga tgaacaggct
     3181 caagagagct gcctgccgac aggtgaacaa cgtagaggga acaatttgcc tgttgtgcca
     3241 tggcaatggc acgttcaaca gccttaacct ccacctgcgg tggacgtgaa agttggtggt
     3301 agataggctg agttttgccc tgccggtaaa aggagtagcg cagcctgtca acctcttcgc
     3361 caagctcgca gtgagcggta accattcctc cattccttcc gactacgccc attacccgtt
     3421 gaatatccgt atcgtgaagg ccgttggtat caaggtaagc catgtagatt ttaaaggatg
     3481 gaaatcctaa atctatgcac cgcttaattt catcctcact ttccctgcgc cagtcaaccg
     3541 gactaacatg aaaggagtaa tccgtgagac actcattagc ttcttctatt cttgcagcta
     3601 acgcctcggc aaggggttgc cccctatgcg gggtaacaaa atcaataata gtggttgtcc
     3661 ctccgtatag ggcagctatg ctacccgtgt gaaaatcgtc cgatgagtag ccagcaggag
     3721 ttggaagatg cagatggacg tgcgggtcaa cacctcctgg aagtatgtac ataccattgg
     3781 cattaatggt ctggaacata tcgtcattaa actcgttggc caatgctgca attttcccgt
     3841 ttttaataag aatatcaact ttttgggtat tagtagaagt aacaaccgtt ccatttttaa
     3901 taatgtattc caatttttca gccatgttgg ttctcgttta aagttttgta aaaatggcca
     3961 tcgctggtaa tgttaaaacc caccttaagt aggtagtttc tgtgcttttc ggtttcagcc
     4021 ttagcccaaa ccctggcaaa accatgctcg gtgaagtttt gcactagcca cgagtaaaca
     4081 aaccttccat ttttaaaatc cctatattcg ggcaaaacgt agtcaagctg aacctccagg
     4141 gttctatcag cgaccttccg ggcaacaaac accccagcca ctaccatgtt acgaagcaca
     4201 aagaagctaa tggtgttctc atcgggggag taggaaaagt tgggaaagta tttctgaata
     4261 tcctttgagt ggtagtctag aaatcgcagt aggtacttgt tgtcgggtct tatttcgaga
     4321 atctcgaatg tgtccttttg ggagtaaatt cttataaggt agtaaaggtc ggtaacaaca
     4381 ataattccat tgagaatacc cactggtagt gcacctatta gaaagccata tattgagaaa
     4441 agtaatgcac cggtgaggtt tacccagcgg aacttgacaa tggagttgag cgccattgaa
     4501 agggcaataa tcaccgaagc cgtgtagcca atccattcca aaatatcagc attcatagga
     4561 ggtagaattt gtttggcaaa ggtaggcaat taatacgtag tgtcagcaaa ttcattcagc
     4621 tcctacatcg cccatacgtt tccataggta ggctgcaatc tttctggatt cgattagtaa
     4681 ggcctcctcg tcaaccagtg ttaatcggtg gtttttaaca ataagcttac cgtttgaaat
     4741 tacatcccgt atgtgcgagg agttaatacc gaaaaccagg tgggcagcaa aattgtcagg
     4801 ggtaaccggg gtgggggagt tgtagtcaat taccaacagg ttcgactcgt catcgccgga
     4861 aaagttgttg gaggaaagat acctatgagc gtttctcaac cggctgtagg cctgcatggg
     4921 gctcatgggg tcgaagtttt gccctacgaa gtaggccgat ttcatactct gcagcatgtc
     4981 gctgtgcatt ccgtcggttc ccagcatgag gttattgccc aggccctttg agttgaagta
     5041 gcctacattg ttgttcaggt tgctatcggt gttctgcacc acccagcatg gggagctggc
     5101 aattatatct cgttcgttac ctgagaggtg gaggcagtga actagaatag acttggagga
     5161 gcttagcaac ccgaagctat ttagcctttc gattaccgat tggccataat tctggtggca
     5221 atgctcctga tcgtatatac cttctgccac atgaat
//
LOCUS       DAOOQX010000047         5253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_18939_length_5253_cov_3.230088, whole genome shotgun sequence.
ACCESSION   DAOOQX010000047 DAOOQX010000000
VERSION     DAOOQX010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18939_length_5253_cov_3.230088"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1387
                     /locus_tag="PLX53_01390"
                     /pseudo
     CDS             1..1387
                     /locus_tag="PLX53_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214983.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
     gene            complement(1457..2074)
                     /locus_tag="PLX53_01395"
     CDS             complement(1457..2074)
                     /locus_tag="PLX53_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOY71765.1"
                     /translation="MNSYIVNLICLVVGYLCGSVLFANIITRLVVGKEIRELGNNNPG
                     AYNVFNNAGKFWGVFTGILDGLKAFVPMLVAHRYFNVSNTGIGLIGVGAVIGHGYPLF
                     FRFKGGRAASSLMGMFLFFIPYELLAAFAIDLILLYTIVKKDFGFWGPFGIIAIAATL
                     CLFFNHPVEVKILIWVGGLLGVYFNRKFIPLRTKQLLNKAPEDDN"
     gene            complement(2087..2965)
                     /locus_tag="PLX53_01400"
     CDS             complement(2087..2965)
                     /locus_tag="PLX53_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOY71766.1"
                     /translation="MSQKIFVISADNLGLSRDQVDYPDLELLRFPVFVGDKEYRQDEV
                     HNAKWLLQKYANEQVVAKSSTLVKQEMVDIVERVRSSYDMIVQVVMGSGMSSASFVVA
                     ENVRKMYEGIIPIVNVDSSQVINGVGNVLLGVIDLIKSNPNLSPEEIAQKSKDVVQNT
                     FTYFIFPDLNYLYRGGRIGKAKSLVGTIMKIIPIIGMLGDNGEGVMVPVGKGRTFKQV
                     NTQLVELIKAKMAEKSVSTIKRMNLTHFGGNDEAVAELKEMVGKLPCNDFIIGTPNLV
                     EAVYAGPGAYCLSMSL"
     gene            complement(2978..4828)
                     /locus_tag="PLX53_01405"
     CDS             complement(2978..4828)
                     /locus_tag="PLX53_01405"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOY71767.1"
                     /translation="MDTELKINEVEVKPHELKVSVNVKEINKRIENIHRYLNSHKEYL
                     NRLNVFPVPDGDTGLNLTLTFQGAMASMQGMENESMLPGEYLKNFTEQMLLNSRGCSG
                     VIFSLFAQGVAQVLENNDFSNENLHRAIANGFKNAWDGTENPREGTMLTLMREFRDIF
                     GKYIQEEKNAAMVISKSIPYLMEVLDRTPEMLPVLKQAGVVDSGGAGFIIILQGIDRE
                     LKHNGSGFNSLSIATLLNISRTTRKLLRMRKSHVRKSSLTPLITNIDDSKIHNSRLRE
                     ILQNARKVINTWNGSRKLPGQEKVISDLEEMGNLWDSDIKLKYCTEFVLEANAINSKE
                     ELREIIGHYGDSLIIVNSGNRYKVHIHTNKPDAVFADAGRYGELVFTKVDDMKKQHRN
                     FVSDDVIEYERDKSILCIVSGSGFADILKNFGADDVLCYGKNKPSVNQLVKKIDGLKA
                     KNVIAAADDSDILMALKYAVSLCKSNVYIVESNNPISIIAMMLGISKEFDIATMFESA
                     MSNLNSITYCEIARSVRNVEADGFTVKKRDFFAVHNKKIIISRTNLEQLVVDVVEKLN
                     PGASLVTIYKGIPSRSDRSLLPILREKFPNLEFEEYYGGQSKFNYYISFE"
     gene            complement(4942..>5253)
                     /locus_tag="PLX53_01410"
     CDS             complement(4942..>5253)
                     /locus_tag="PLX53_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOY71768.1"
                     /translation="HRLRPCHNPDLSSVIHLVGRCGGKYGFISAHAANSFGVATLVSF
                     IFYKRWATIGMLVWATFVSYSRIYLGVHYPGDVLVGAIVGSAIGIAVWLVAERLINRR
                     N"
BASE COUNT         1458 a         1285 c         1136 g         1374 t
ORIGIN      
        1 cccaacgata gcactcccat atcctacggt ttaaactcta aactggtgaa aaaaaacgga
       61 aaaatagagg agcaggtttg gtatgcaaac ggcatgtacg gaaaggctat cggtaaaatc
      121 gttttctggc tggagaaggc cgccacagtg gctgaaaatg aatatcagca aaaaataatt
      181 gaaaccctgg tagattacta caaaacaggc gatttgagaa agtttgacga gtacaacata
      241 ctctggctaa gctatacaca gtctaaagtt gattttgtca acggtttcat cgagacttac
      301 ggcgatccgc tgggctacaa gggaagctgg gagtcggtgg tgaacttcag gaacgacgag
      361 gcaacccatc gtaccgaggt gataagcgcc aacgcacagt ggtttgaaga tcactcgccc
      421 attgacagca tattcaaaaa gaaggaagtg aaaggggtaa gcgccaaggt gataaccgtg
      481 gcccagctcg ggggcgattg ctacccgtca acccccattg gcataaacct gccaaatgca
      541 gactggataa gaaagcagca tggttcaaaa tcggttacca tggataatat cacctacgcc
      601 tacaaccaag cctcgttggg aaatggattc atggaggagt ttgcctggtc ggacgaggag
      661 gtagcactca acaagcagta tggttacctg gccgattgcc tgcacaccga tttgcacgaa
      721 tgccttgggc atggctctgg ccagctggct ccaggagtta aaggtgatga gctgaaaaac
      781 tacggctccc cattagagga ggctcgggct gacctatttg ccctttacta tatcatggat
      841 ccaaaaatgg ttgagctggg cctcatgccc aaccttgata gccagggttc attacagcaa
      901 ctacatccgc aacggattaa tgacgcagct aacccgggtg gaacctggga aaaacattga
      961 gcaggcacac atgcgcaacc gccagctgat tgcccaatgg tgctacgagt atggaaagga
     1021 tgaaaatgtt gttgagagga aaaaccgaaa tgggaaaacc tactttgtgg taaacgattt
     1081 ccagaagtta cggcagctat ttggcagcct tctgcgtgaa attcaacgga ttaagtcttc
     1141 gggcgattac gagtcgggca aaaaacttgt tgagagcttc ggggtaaaag ttgaccccgc
     1201 gctgcaccac gaagttctgg agcgctattc caagttgaat ttggccccct acgggggatt
     1261 tatcaaccca gaccttgtgc ctgtagttca aaacgacagc attgtggacg ttagggttga
     1321 ttatccagag gattatgcca ggcagatgct ggagtactcc gaacgctact cgttcctaac
     1381 gaagtaaacc atcaggggtc atcagcctac cggctgatga ccccatgcga ctgaccacag
     1441 caagtaccaa ttttagctaa ttgtcatcct cgggcgcctt attgagcaac tgcttggttc
     1501 ggagggggat gaatttcctg ttgaagtaaa cgcctagcaa accacccacc cagattaaaa
     1561 ttttcacctc aacgggatgg ttgaaaaaca ggcaaagggt tgccgcaatg gcgattattc
     1621 caaaaggtcc ccagaaccca aaatctttct taacaatggt gtagagcagt ataaggtcaa
     1681 tggcaaaggc tgccagcagc tcgtagggta taaaaaagag aaacatgccc atgagcgatg
     1741 aggctgccct acccccttta aacctgaaga aaagcgggta gccatgtcca atcaccgcgc
     1801 ctactcctat taaccctata ccagtattgg aaacgttaaa gtacctatgg gccaccagca
     1861 tgggaacaaa ggcctttagc ccatccagaa tgcccgtaaa aacgccccag aacttaccag
     1921 cattgttaaa aacattgtag gccccggggt tgttgttccc caactcccta atttcctttc
     1981 ctacaaccag cctggtgata atattggcaa acaacaccga accgcacagg taacccacaa
     2041 ccaggcaaat aaggttaact atatagctgt tcataacatt aaaaatttat agtgacatgg
     2101 acaagcagta agctccaggt ccggcatata ctgcctctac caggttcggg gtaccaataa
     2161 taaagtcgtt gcatggcaac ttcccaacca tttcctttag ctcggcaacc gcttcatcgt
     2221 ttccaccaaa atgggtaagg ttcatgcgct taatggttga tacagatttt tcggccattt
     2281 tggcctttat cagctcaacg agctgggtgt ttacctgctt gaatgttcta ccctttccaa
     2341 cgggaaccat aacaccttcg ccgttatcac ctagcatacc aataatgggt ataattttca
     2401 taatggttcc aaccagcgat ttagcttttc cgattcttcc tcccctatac aagtagttaa
     2461 ggtcagggaa aatgaagtag gtaaatgtat tctgcacaac atccttcgac ttctgtgcaa
     2521 tttcttcagg acttaagttg ggattgctct taattaaatc aataacgcca agtagaacat
     2581 taccaacacc atttatcacc tggctgctgt ccacattcac tatcgggata atgccctcgt
     2641 acatcttgcg cacgttttcg gcaaccacaa acgaagcgct tgacatgccg cttcccatca
     2701 ccacttgaac aatcatgtca tagcttgaac gaacccgctc cacaatatcc accatttcct
     2761 gcttcaccag cgtggatgat ttggcaacca cctgttcgtt ggcgtacttc tgcaaaagcc
     2821 atttggcatt gtgcacctca tcctgtcggt actctttatc ccctacaaat accgggaacc
     2881 tgagcagctc caaatcgggg tagtcaacct gatccctcga aagcccgagg ttatcggcac
     2941 taatcacaaa tatcttctgg ctcatagttt ttaaagttta ttcaaagcta atatagtagt
     3001 taaacttact ttgtcctccg tagtactcct caaattcgag gtttggaaat ttttccctaa
     3061 ggattggcag caggcttcta tcgctcctgg aaggaatccc tttgtatatc gtaaccagac
     3121 ttgctcctgg gtttagcttt tcaacaacgt caaccacaag ttgctccagg ttggttcggg
     3181 aaataataat tttcttgttg tgaacggcaa agaaatctcg ctttttaact gtaaaaccat
     3241 cggcctcaac atttctgaca gacctggcta tttcgcagta ggtaatagag ttcaggttgc
     3301 tcatggccga ttcgaacatg gtggcaatgt cgaactcctt cgaaattcca agcatcatgg
     3361 caataatgga aatagggtta ttcgactcca caatatacac gttcgattta cagagcgaaa
     3421 cggcgtactt aagcgccatc aggatgtcgc tatcatcggc agctgctata acgtttttgg
     3481 cctttagccc atcaatcttt tttaccagct ggttaaccga gggcttgttc tttccatagc
     3541 acagcacgtc atcggcacca aaattcttaa gaatatcagc aaacccgctg ccggagacga
     3601 tgcagagaat acttttatcc ctttcatatt caataacatc gtcgctcaca aagttgcggt
     3661 gctgcttttt catgtcgtcc accttggtga aaaccagctc gccatacctg ccagcatcgg
     3721 caaaaacagc atcgggctta ttggtatgga tatgcacctt ataccggtta ccgctgttaa
     3781 caataattag gctatcgcca tagtgaccaa taatttctct aagttcttcc ttagagttga
     3841 tggcattggc ctccagcaca aattcggtgc agtactttaa ttttatgtcg gagtcccaga
     3901 gatttcccat ttcttccaaa tcgcttatta ccttttcctg gccaggcaac tttcggctac
     3961 cattccaagt gtttataacc tttcgtgcat tctgaagaat ttcgcgcaac cgggagttgt
     4021 gaattttgct atcatcaata ttggtaataa gtggcgtaag ggaacttttg cgaacatgag
     4081 actttctcat ccgcaaaagc ttacgggtgg ttcggcttat gtttagcagg gtagctatgg
     4141 acaagctgtt aaaacccgaa ccgttgtgct ttagctcacg gtcgataccc tggaggatga
     4201 tgataaaacc tgcacctccg gagtcaacca cgcccgcttg tttaagcacg gggagcattt
     4261 cgggggttct gtcaagcacc tccatcaagt aggggatcga cttgctgatt accatagcag
     4321 cattcttctc ctcctgaatg tacttgccaa atatatccct aaattcccgc atcagggtaa
     4381 gcatggttcc ctcgcgggga ttttcggttc catcccaggc gtttttgaaa ccattggcaa
     4441 tagccctgtg gaggttttca tttgaaaaat cattattttc cagaacctgg gcaactccct
     4501 gggcaaacaa tgaaaaaatc acacctgaac aaccccggct attcagcagc atttgctcgg
     4561 taaaattttt aagatactcg ccgggaagca tggattcgtt ctccataccc tgcatgctgg
     4621 ccattgcacc ctgaaaggtt agcgttaggt ttaacccggt atcgccgtcg ggtaccggga
     4681 aaacatttag cctattcagg tactccttat ggctgttcag gtagcgatga atattttcaa
     4741 tccttttgtt tatctcctta acattaacgc ttaccttcaa ttcgtgaggt tttacctcta
     4801 cctcatttat ttttagttct gtatccataa caaccagcta attgtcaatt tggtttgctt
     4861 ttatgaatgt gcggataaac ttttaaaact tctaacgtaa gtcctccggc aaaaataaaa
     4921 atatttaaat tgaaaatatc cctagtttct tcggtttatg agcctttctg caaccagcca
     4981 cacggcaata cctattgctg aacctacaat agctcccacc agaacatcgc cgggataatg
     5041 aacgccgagg tatattcggc tgtaggagac aaaagttgcc caaaccaaca ttccaatagt
     5101 agcccagcgc ttatagaata tgaaacttac cagcgtggcc acaccaaacg aattggcggc
     5161 atgggctgag atgaatccat acttcccccc acaccgtcct accaggtgaa ttacactact
     5221 taggtcgggg ttatggcaag gacgcagcct atg
//
LOCUS       DAOOQX010000048         5174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_19316_length_5174_cov_3.299082, whole genome shotgun sequence.
ACCESSION   DAOOQX010000048 DAOOQX010000000
VERSION     DAOOQX010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19316_length_5174_cov_3.299082"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /gene="murI"
                     /locus_tag="PLX53_01415"
     CDS             complement(<1..631)
                     /gene="murI"
                     /locus_tag="PLX53_01415"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOY71769.1"
                     /translation="MLGSNPIGIFDSGSGGLTVLYELLKVLPRESFVYFADSANCPYG
                     PKPQSEVIELSDCITRFLLEQGCKLVVVACNTATAAAIEHLRSNFKIPFIGMEPAIKP
                     ASLNSKTKSIGVLATAGTFKGKLYIETSKKYASDVNVCYQVGEGLVELVEAGKADSPE
                     AAELLHRYVDPMVDCNIDHLVLGCTHYPFLMNSLSRILPRGVEIVNPAPA"
     gene            complement(808..1326)
                     /locus_tag="PLX53_01420"
     CDS             complement(808..1326)
                     /locus_tag="PLX53_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOY71770.1"
                     /translation="MRKISTIAFILFLSATGVKTYAQSYKFGHINSQEILSVMPERDS
                     AVAKLDKFTKTLQDQVEELQVEFNKKYQDYLQKRSTFTDAIREMKEKELTEMQQRAQE
                     FQQVAEQDYQRYQGEVMKPVIEKADSAIKKVAKANGFTYIFDTSTGVILYFSEQSVDI
                     GPLVKKELGITK"
     gene            complement(1365..1874)
                     /locus_tag="PLX53_01425"
     CDS             complement(1365..1874)
                     /locus_tag="PLX53_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOY71771.1"
                     /translation="MKKILLLPIVLLISTSLWAQKFAFVDSEYILNKIPSYKAAQEQL
                     DKLSEQYQKEIEGKYAEIDKMYKDYQTEKVLLTQEMQKKREDEIISRERQTKELQMKY
                     FGRDGLLFKKREELVKPIQDQVFNAVKEIAIEGGYAIIFDAAASPNMLYTNPRYDKSE
                     DVLLKLGYK"
     gene            complement(2061..4538)
                     /gene="bamA"
                     /locus_tag="PLX53_01430"
     CDS             complement(2061..4538)
                     /gene="bamA"
                     /locus_tag="PLX53_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOY71772.1"
                     /translation="MLKSKIFLLLLLLSSVSALAQEPSFSYEVPKKYCVKEVRVSGIK
                     FLNPDVLVSVSGISVGDSILVPGDAVTKAIKKLWSQGLFSDIKISATKIENSDIYLDI
                     FLQEQPRISSINFVGVRKGEVSDLKEKLKLRNGSQLTESILKNSVEIIRKHYRTKGFL
                     NVDVNPIQENDTALANMVKLTFDIRKNERVKIDQITFDGNTAFTDARLRKSLKKIHRR
                     DINIFKSAKFIQSDYDESKDNLIAFYNENGYRDSKILKDSVFTLNNKRIGINFLVHEG
                     PQYHIRSISWIGNTKLPSEALTAILGMKKGDVYDKTLLEKRLFTDDNSISTVYMDDGY
                     LFFNLDPVESQIQNDSVDLEMRIYEGDQATINKVLIVGNTKTNEHVIRRELWTKPGYL
                     FSKSEITRTIRELGQMGHFDPEKLDVQPMPNPAEGTVDLKYVVEEKANDQLEISGGWG
                     NKMFVGTVGIRFTNFSVRRLFDKEAWRPVPSGDSQSLSLRASTNGTYYKAFSFSFTEP
                     WLGGRKPTNFSFSVYHTIQNGGVDYFYQTSDKYFKVTGASVGIGTRLKWPDDYFSIYN
                     EISYQNYNLKDWTGYFLFSNGQSNNLSYKISLTRNSTDQVIYPRTGSNLSLSLQITPP
                     YSLFNGKNYSAFSGSEKYKWIEYHKWTGRIQWYVPLVEKLVLYSNFQFGIMGYFNSDI
                     GHSPFEGFDVGGDGMSGYNLYGKETIGLRGYKNGSLTPYTLIDGSYVGDGHVYDKYTV
                     EIRYPLTLNPQAAIYLLTFFEAGNAWRDASEFNPFNVHRSAGIGARMFLPMLGMLGID
                     WGYGFDKIPDNPDAHKGQFHFIIGMPF"
     gene            complement(4676..>5174)
                     /gene="uppS"
                     /locus_tag="PLX53_01435"
     CDS             complement(4676..>5174)
                     /gene="uppS"
                     /locus_tag="PLX53_01435"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOY71773.1"
                     /translation="MNLLIETIDNEIGNLNKNDVRLITIGNTSALPKNVQTKLKYAID
                     KTSQGKTLTTILALNYGARAEITEATRKILTEYRNGKLDEMSLSPEVFGGYLQTAGIP
                     DPELLIRTSGEIRLSNFLLWQLAYAELYFTPVLWPDFSKDDFFNAIVEYQHRERRFGM
                     TSEQL"
BASE COUNT         1389 a         1127 c         1147 g         1511 t
ORIGIN      
        1 cagcgggtgc aggattaaca atttcaaccc ctctaggcag tatccggctt agggagttca
       61 tcaaaaaagg gtaatgggtg caccctaaaa ccaaatggtc gatgttgcaa tctaccattg
      121 ggtcaacata cctatgaagg agctcggcag cttcgggcga atcggccttg ccggcctcaa
      181 ccagctcaac caaaccctct cccacctggt agcagacatt cacatcagag gcatattttt
      241 tactggtttc gatgtaaagt ttccccttaa aagttccggc agttgccaac acgccaatac
      301 tcttggtttt ggaattaagt gaggcgggtt ttatagccgg ctccatgcca ataaagggaa
      361 ttttaaagtt acttcgaagg tgctcaatag ctgcagcggt tgcggtgttg catgcaacaa
      421 ccaccagctt gcaaccctgt tccagcaaaa aacgggtaat gcagtcagaa agttcaatca
      481 cctccgactg gggtttgggc ccgtatgggc aatttgccga atcggcaaag taaacaaagg
      541 attcccgggg cagtactttt aaaagttcat acagcactgt taatccgcct gacccggagt
      601 caaaaattcc tataggatta ctaccaagca attaacgata accttaattt gggaaaaatg
      661 aaatattcat gacaatagcc caggcagatg ctaacgatta ctccatggat tacccttctg
      721 agccatagaa gaacaaaata taaataaatg aaaaaaggga taaaccgcat acgtcggcaa
      781 atccctagta taagccatca gggctgatta tttcgttatc cccagctcct ttttaaccaa
      841 tggacctata tcaacacttt gctccgaaaa gtaaaggatg accccggtgc tggtatcaaa
      901 tatgtaggta aacccgttag ccttggcaac ctttttaatt gcagaatctg ctttttcaat
      961 gaccggtttc ataacctcac cctggtagcg ttgatagtcc tgctcggcta cctgctgaaa
     1021 ctcctgagct ctttgctgca tctcggtaag ctccttctcc ttcatctcgc ggatggcgtc
     1081 agtaaaggtg ctgcgctttt gaaggtaatc ctgatacttc ttattaaatt caacctgaag
     1141 ctcctcaacc tgatcctgca acgtcttggt aaacttatcc agtttggcaa cagcgctatc
     1201 cctctcgggc ataacactta gaatctcctg gctgttgatg tgcccaaact tatagctttg
     1261 cgcataggtt ttcacaccag tggctgataa aaaaagaatg aaagcaatcg tggatatctt
     1321 tctcatgatt atcaattttt aagtaagcaa atataaaaat taaattattt ataccctaat
     1381 tttaggagca catcttcact tttatcgtag cggggattgg tgtaaagcat gttgggtgat
     1441 gctgcagcat cgaaaataat ggcatacccc ccttcgatgg ctatctcctt aacggcatta
     1501 aaaacctgat cctgaatggg tttaaccagc tcttcccttt tcttaaagag taacccatcg
     1561 cgaccaaagt acttcatctg cagctctttg gtttgccttt ccctggaaat tatctcatct
     1621 tcacgttttt tttgcatttc ctgggttaaa aggacctttt cggtttggta gtccttatac
     1681 atcttgtcaa tttcagcata cttgccctca atctcctttt ggtactgctc cgagagctta
     1741 tcaagctgct cctgggctgc cttataggaa ggaattttat tgaggatgta ctctgaatcg
     1801 acaaaagcaa atttttgtgc ccacaagctt gtgctaatca gcaagacaat tgggagtagg
     1861 agtatttttt tcattggtgt gtaatttatt taaacttaaa tgcgactact ggttgcgagt
     1921 aaaattaagc tggtcatttt caaatatcta aagtatcaaa agtaatcggc taagtatttc
     1981 aagttgaagc tgaattgtta aggatatatc aacatgcctg ccaattattg aaatagggtt
     2041 gatataaggg ttaagaattg ttaaaatggc attccgataa taaagtgaaa ctgcccttta
     2101 tgggcgtcgg gattgtcggg aattttatcg aatccgtatc cccagtcaat tccaagcatc
     2161 cccagcatgg gaaggaacat tcttgcgcca atgcctgccg agcggtgcac gttgaagggg
     2221 ttgaactcgc tggcatcacg ccatgcattt ccagcctcaa aaaaggttag taggtatatg
     2281 gcggcctgtg ggttaagggt taaagggtag cggatttcaa cggtgtactt gtcgtaaaca
     2341 tggccgtcgc caacgtagct accatctatc agggtgtacg gggtaagaga accattcttg
     2401 tacccacgca ggccaattgt ttccttgccg taaaggttgt agcccgacat accatcaccg
     2461 cctacatcaa atccctcaaa gggtgaatgg ccaatatcgc tgttaaagta acccataatt
     2521 ccgaactgga agttggagta gagcaccagc ttttcgacca ggggaacata ccattgaatt
     2581 cgcccggtcc atttatggta ttcaatccat ttgtactttt cgcttccgga aaatgccgag
     2641 tagttctttc cattaaaaag cgagtatggt ggggtaattt gaagtgacag gctaaggttg
     2701 gatcctgtac gggggtagat aacctgatcg gttgagttgc gcgtaaggga aatcttgtag
     2761 cttaggttat tcgactggcc gttgctgaaa agaaagtatc ccgtccaatc cttcaggtta
     2821 tagttctggt agctaatttc gttgtaaatg ctgaagtagt cgtcgggcca tttcaagcgg
     2881 gttccaatac ccacagaagc tccggttacc ttaaaatatt tatcactcgt ctggtagaag
     2941 tagtccactc cgccattctg gatggtgtgg taaaccgaga aggaaaagtt ggttggtttt
     3001 ctaccaccca gccagggttc ggtaaaggag aagctgaagg ctttgtagta ggttccgtta
     3061 gtagaagctc taagtgatag ggattggcta tcacccgaag gaacaggcct ccatgcctcc
     3121 ttgtcaaaga gtcttcgaac ggaaaagttg gtaaacctta tgccaaccgt tccaacaaac
     3181 attttgttac cccatcctcc agatatctcg agctggtcgt tggccttttc ctccaccacg
     3241 tatttaagat cgaccgttcc ctcagcggga tttggcatgg gctgcacgtc gagtttttcg
     3301 ggatcgaaat gtcccatctg gccaagttcc ctgatggtgc gtgttatttc cgatttgctg
     3361 aacaggtagc ccggtttggt ccaaagctca cggcgaatta cgtgttcatt tgttttggta
     3421 ttgccaacaa tcagcacttt gttaatggtg gcctggtcgc cctcgtatat tctcatctcc
     3481 aggtcaacgg agtcgttctg aatctggctt tcaaccggat cgaggttgaa aaacaggtag
     3541 ccatcatcca tgtaaacggt cgaaatggag ttgtcgtcgg tgaaaagcct cttttccaga
     3601 agggttttat cgtaaacgtc gcccttcttc attcccagta tggcagtaag ggcttcggag
     3661 ggaagcttgg tattaccaat ccaggaaatg ctgcgaatgt gatactgtgg cccctcgtga
     3721 acaagaaagt taatgccaat tcgcttgttg ttcaatgtaa aaacggaatc cttcaaaatt
     3781 ttggaatccc ggtaaccatt ctcgttgtaa aatgctatca ggttatcctt gctctcgtcg
     3841 tagtcggact gtatgaattt tgccgattta aaaatgttga tatcacggcg gtgaattttt
     3901 ttaagggatt tacgtaggcg ggcatcggta aatgctgtgt tgccgtcaaa tgtaatttgg
     3961 tcaattttca ccctttcatt ttttcttata tcaaaagtaa gcttaaccat gttggccaaa
     4021 gcggtatcat tttcctgaat gggatttaca tctacattca gaaatccttt agtccggtag
     4081 tgcttcctga taatctccac gctattctta aggatgctct cggttagctg gcttccgttg
     4141 cgtagcttca acttctcctt aaggtcgcta acctcacctt ttctcactcc aacaaagttg
     4201 atagacgaaa tacggggttg ctcctgaaga aaaatatcga ggtagatatc tgaattttca
     4261 atttttgtag ccgaaatttt aatatcggaa aatagaccct ggctccatag ctttttaata
     4321 gccttggtta cggcatcacc agggacaagg attgaatcgc caacgctaat acccgaaact
     4381 gataccagca catcgggatt gagaaacttg atacccgaca ccctaacctc tttgacacag
     4441 tattttttag gtacctcgta gctgaacgag ggctcctgag ccagagcgga aacgctgcta
     4501 agaagaagta gaagtaaaaa tatttttgat tttagcatac tgcaattttg ctaattaata
     4561 ctggggtgta aaaataatat attttcaatt gccaaagttc tatatacaaa taagaggctg
     4621 ggttcaaaag taggtaaatg accctcaggg aacaagctaa aaaatgcgaa ttgagctaaa
     4681 gctgctcgct ggtcattcca aaacgccttt ccctgtgctg gtactcaaca atggcattaa
     4741 aaaaatcgtc tttgctaaag tcgggccaga ggacaggggt aaagtagagc tcggcgtagg
     4801 ctagctgcca tagtagaaaa ttgctaaggc gaatttcgcc gctagttctg atgagtagtt
     4861 ccggatcggg tattcccgct gtttgcaggt acccgccaaa aacttcaggc gataggctca
     4921 tctcgtcaag ttttccattc cggtattcgg taaggatttt tcgtgtagcc tcggtaattt
     4981 cggctcttgc cccatagttt agcgctaaaa tggttgtcag ggttttcccc tgcgaggttt
     5041 tatctatggc atattttagc ttggtttgaa cgttttttgg aagggccgat gtgtttccta
     5101 tggtaatgag gcggacgtca ttcttattaa gattacctat ttcattatca atggtttcaa
     5161 tcaacaagtt catg
//
LOCUS       DAOOQX010000049         5128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_19553_length_5128_cov_3.184506, whole genome shotgun sequence.
ACCESSION   DAOOQX010000049 DAOOQX010000000
VERSION     DAOOQX010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19553_length_5128_cov_3.184506"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..938)
                     /gene="prmC"
                     /locus_tag="PLX53_01440"
     CDS             complement(78..938)
                     /gene="prmC"
                     /locus_tag="PLX53_01440"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOY71774.1"
                     /translation="MKSYTQRQLFDEVGLVLNQFFTSGEVNTLRRMLLEHVLKIPYYH
                     VHLTPNAQVSPSLSKNILDMTGQLVRGVPIQYLLGETTFMELNLTVNPSVLIPRPETE
                     ELVRWVIADCHLPKPKILDIGTGSGCIAIALAQNIPDSTLTAVDISPDALKVARHNAK
                     IYSAAICFVEADILSGSLIPNAPFDIVVSNPPYVRSSERRFMHKNVLDHEPPLALFVS
                     DDNPLQFYKAIVSKSSKLIKQGGRLFLEINEEFGNQMVELLEANHFGKVTLRKDINER
                     YRMISGTYTG"
     gene            982..2079
                     /gene="ribD"
                     /locus_tag="PLX53_01445"
     CDS             982..2079
                     /gene="ribD"
                     /locus_tag="PLX53_01445"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOY71775.1"
                     /translation="MDNLDVDKLYMSRCLELALNGMGSVAPNPMVGAVVVHNGKIIGE
                     GFHAQYGEAHAEVNAINSVRDKSLLQDSTLYVSLEPCSHYGKTPPCTDRIVEYGIPRV
                     VVATTDPNPRVSGRGLEQLRKHGVEVSLGILEDEALLLNRRFITFHTCKRPYIILKWA
                     QTIDGFIDLVRTAETPVGSVWITNELARSLVHRWRSEEQAVIVGTNTVERDNPRLNIR
                     SWSGRNPIRVIVDRKLRLSRDANVFDGSQPTILFIGNNSSALVRKSEFAGISNLEMVT
                     IDFAKGSEQQILKELSDRDVISLIIEGGATLLNNFIQKNLWDEARVFVGNRFFGEGVK
                     APLFKGDPVSYEEVGDSKLFIYRNQQGRSNR"
     gene            complement(2159..2734)
                     /locus_tag="PLX53_01450"
     CDS             complement(2159..2734)
                     /locus_tag="PLX53_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOY71776.1"
                     /translation="MRISVYCASSSKINQKYFDATRQLASELANRGDIIVYGGGSHGL
                     MGCLADTALMNGGMVVGILPRFMNKVEWGHKNLTELKLVKDMHERKKLLIKDVDAVVA
                     LPGGCGTLEELMEVITLKRLGKFTKPIIILNTDGFYDYLKLLLAKMIDEHFMRPEHAG
                     IWTFVETPQEVIPAIEKAPTWDSDAIKFAAL"
     gene            complement(2997..3317)
                     /gene="trxA"
                     /locus_tag="PLX53_01455"
     CDS             complement(2997..3317)
                     /gene="trxA"
                     /locus_tag="PLX53_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOY71777.1"
                     /translation="MAQEVNDSNFDEVVLNAGKPAVVDFWAEWCGPCRMIAPYIEQIA
                     DEYKDRAVVAKVDVDNNPGIATRFGIRNIPTVLFFKDGQLADKQVGAVPKSNLVSKLE
                     ALLR"
     gene            complement(3393..>5128)
                     /gene="dnaE"
                     /locus_tag="PLX53_01460"
     CDS             complement(3393..>5128)
                     /gene="dnaE"
                     /locus_tag="PLX53_01460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOY71778.1"
                     /translation="QLDIDNIPLDDSTTLELFSRGDTTAIFQFESAGMKKYLKALEPN
                     RFEDLIAMNALYRPGPMEYIPSFINRKHGREKIEYDIPEMEEFLKDTYGITVYQEQVM
                     LLSQKLAGFTRGEADSLRKAMGKKLRDVMDKMKGKFIHGCINNGHDQAKVEKIWTNWE
                     AFAEYAFNKSHSTCYAFLAYQTAYIKAHYPGQFMAAVLSRNLSDIKKITTFMDECRRM
                     GLQVLGPDVNESNYKFSVMPNGDIRFGLGAIKGMGENAAANIIETRNHEGKFKNIYDF
                     MERVNLQTVNKKNLESLVLAGAFDNISPISRGAYFTPDSKGVTFMETLMRYGAKVQLE
                     KNSKQMTLFGATNGFEVPQPEPPKTDDWPTIEKLNKEKEIIGIYLSAHPLDEFRFEIE
                     NLCNTNLAELKNLEDFNNREVTIAGMVVKAKIDTTKNGKPYARLTIEDYSDTMDIMLF
                     GKDFETYRNFCYTGYMVMIKGKVQPKAFKPEELELHIKSMSMLYDVREKQIKGISITL
                     PTTEINDILIDDFTRLTHENAKVSLRFKVVDVENRISVDLFSRSHRVSINTELIHFLA
                     DNELEYKLITS"
BASE COUNT         1312 a         1125 c         1220 g         1471 t
ORIGIN      
        1 ggttttggag ccgtgtttaa gctttatcaa tactcacttt ttctctgcta tccataatta
       61 aagtgggttg attatgttta gccggtatag gtgcccgaaa tcatccggta gcgttcattt
      121 atatcctttc gcaacgtaac cttgccaaaa tggtttgcct ccagcagctc taccatttgg
      181 ttgccaaact cctcattaat ttcgaggaat agccttcccc cttgcttaat caacttggag
      241 gacttgctta caatagcctt gtaaaattga agggggttat catcacttac gaacagggct
      301 agcgggggtt cgtggtcgag cacatttttg tgcataaacc tcctttcgga gctgcgcacg
      361 tagggtgggt tgctcaccac aatgtcgaag ggagcgtttg gaatcaggct gccggaaagg
      421 atgtcagcct ctacaaagca aatcgcggcg ctgtatattt tagcgttatg ccttgcaact
      481 ttaagcgcgt ccggggaaat atcaacagct gtaagggtag aatcggggat gttttgtgcc
      541 agggcaatgg caatgcaacc cgagccggtt ccaatatcca gaattttagg ttttggtaag
      601 tggcaatcag caatcaccca tcgaacaagt tcttcggttt ccgggcgggg aatgagcacc
      661 gatgggttaa cagttagatt taactccata aaagtggttt cgcccagcaa gtattgaatt
      721 ggaacgcctc gaaccagctg gcctgtcatg tctaatatat ttttggacag gctggggctg
      781 acttgggcgt ttggggttag gtgaacatgg tagtagggaa ttttaagcac atgttccagc
      841 agcatcctac gcaaggtgtt tacctcaccg gaagtaaaaa attggttcag gactaagccc
      901 acctcatcaa atagctgcct ttgagtgtaa ctcttcattt aaaaatttaa ctttgtcata
      961 gcacaaattt aggtaatatt tatggacaac ctcgatgtgg ataagcttta catgtctcgc
     1021 tgcctggagt tggccttaaa cgggatggga agtgttgctc caaaccccat ggtgggtgct
     1081 gttgttgtac acaacggtaa aatcatcggt gagggatttc atgcgcagta tggtgaggca
     1141 catgccgaag ttaatgccat taactcggtt agggataaat cgctgttgca ggattcaacg
     1201 ctttacgttt cgcttgagcc ctgctcgcat tatggtaaaa ctcctccatg taccgaccga
     1261 attgttgagt atggcattcc gcgggtagtg gtggccacaa ccgatccaaa ccccagggtc
     1321 tcgggccgcg ggttggagca gctgcgtaag catggagttg aggtttctct gggtattctt
     1381 gaggatgaag cattgctgct aaacaggagg tttatcacct ttcatacctg caaaagaccc
     1441 tatattattc ttaaatgggc tcaaactata gatggattta ttgatctggt tagaactgct
     1501 gaaacacctg ttggttctgt ttggataact aacgagctgg cacgctcgct ggtacataga
     1561 tggagaagcg aagagcaggc agtgatcgtc ggaaccaaca ccgttgaaag ggataaccca
     1621 cgcctgaaca tccgcagctg gagtggaaga aatcccatta gggtgattgt tgatcgaaag
     1681 ttgcgcctga gtagggatgc caatgttttc gatggaagtc agcctacaat tttgttcatt
     1741 gggaataaca gcagcgcgct ggtgcgtaaa tcggaatttg caggcatctc aaacctggag
     1801 atggtaacaa tcgactttgc caaaggctcc gagcagcaaa tcctaaaaga gctgagcgat
     1861 cgggatgtta tttcattgat aattgaaggc ggtgcaacgc tgctcaacaa cttcattcag
     1921 aaaaacttat gggacgaggc acgggtattt gttggcaacc gcttttttgg cgagggagtg
     1981 aaggcacctc tttttaaggg cgatcctgta tcctacgaag aggtagggga cagcaagcta
     2041 ttcatttatc ggaatcagca gggaagaagc aacaggtgaa atactaataa ccttcagcat
     2101 atcgtttcaa atttaccatt gtagttttgc agtgccaggc gtaaccgcgt tgcaaaagtt
     2161 acagtgctgc aaatttaata gcatccgaat cccatgttgg cgctttttct atggccggaa
     2221 taacctcctg gggggtttcc acaaatgtcc aaatacctgc atgttcaggc cgcatgaagt
     2281 gctcatcaat catcttggcc aaaagtaatt ttaggtaatc gtagaatcca tcggtattga
     2341 gaatgattat gggtttcgta aattttccca acctttttag ggtgattacc tccatcagct
     2401 cctcaagcgt tccacagccg ccaggcaggg cgactacggc atcaacatcc ttaatgagga
     2461 gctttttccg ctcatgcata tccttaacca gctttagctc ggtgagattc ttatgtcccc
     2521 actcaacttt attcataaat cggggcaata tacctaccac catgcctcca ttcataagag
     2581 cggtatcggc cagacatccc ataagcccgt gagaaccacc accatacact atgatgtcgc
     2641 ctctgttggc caattccgag gctagctgac gggttgcatc aaaatacttt tggttaattt
     2701 ttgagctgga cgcacagtaa accgaaattc tcataaggaa aagattaaat gggctctacg
     2761 tttgcaagaa catgcggtta gtcctttatt tcgggaatgc cgctgattgt aatgcacagc
     2821 tgatggagaa taccggttag aatctattga aaactccatt tgctgcaacg cgaatggtgg
     2881 aagggggatt ttgggtaaag ataataatga agatgtttag tagaaatagg taaaaccacc
     2941 acaacatcgg gttaataacc ctaaacatca tggtggtatg aaaattttta aaaaaactac
     3001 ctgagtaggg cttcaagttt ggaaaccagg ttgcttttag gaacagctcc aacctgcttg
     3061 tcggccagct gtccatcttt aaagaacagc acggtaggga tattcctgat gccgaaacgc
     3121 gtagcaatgc cagggttatt atccacatca acctttgcaa caacagctct atccttgtat
     3181 tcatcggcaa tttgttcgat gtagggggca atcattcggc agggtccaca ccattctgcc
     3241 caaaaatcaa ctacagcagg tttgccggca ttcagcacca cctcgtcaaa gttggaatcg
     3301 tttacttctt gtgccatgac aactattgtt ttaaattgat aatctaattc gtttaccagt
     3361 acaaaattaa aacgaaaatt aataccaaag gttcagcttg taattagttt atactccagc
     3421 tcattatctg ccaggaagtg aataagttcg gtgttgatgc taacccggtg cgatcgggaa
     3481 aacaggtcaa ccgaaatcct gttttccaca tctaccacct taaatcgaag cgataccttg
     3541 gcattctcgt gggttaacct ggtaaaatcg tcaataagta tgtcgttaat ttcagtggtg
     3601 ggaagggtaa tcgaaatacc ctttatttgt ttttcccgaa cgtcgtaaag catgctcata
     3661 cttttgatgt gaagctccaa ctcctctggt ttaaaggctt tgggctgaac ttttccctta
     3721 atcatcacca tgtaaccggt gtagcagaaa tttcgatagg tttcaaagtc ctttccaaac
     3781 agcatgatgt ccatggtgtc ggagtagtcc tcaatggtga gcctggcgta aggtttgcca
     3841 tttttggtag tatcaatctt agccttaacc accataccgg caatggttac ttccctgttg
     3901 ttgaaatctt cgaggttttt aagttcggcc aggttggtgt tgcataggtt ctcaatttca
     3961 aaccgaaact cgtcaagtgg atgagcagag aggtaaatgc caatgatttc cttttccttg
     4021 ttcagctttt cgatggttgg ccaatcgtcg gttttgggag gttcgggttg tggtacctca
     4081 aatccgttgg ttgcaccaaa aagggtcatc tgcttactgt ttttttccaa ttgaaccttt
     4141 gctccgtagc gcatcagggt ttccatgaag gttacaccct tggagtcggg agtaaaatag
     4201 gcgccccggc taatggggct aatgttgtcg aaagcgccag ccagcacgag cgattccaaa
     4261 tttttcttgt taactgtttg gaggttaacc cgctccatga aatcgtatat atttttaaat
     4321 ttaccctcat ggttgcgagt ttcgataatg ttagctgctg cattttcgcc cattcccttg
     4381 atagctccca agccgaatct gatgtcgccg ttgggcatca ctgagaactt gtagttgctc
     4441 tcattcacgt ccggtcccag cacttgcagg cccattcgac ggcactcgtc catgaaagtg
     4501 gttatttttt taatatccga aagattccgg cttagcacag ccgccatgaa ctgacccggg
     4561 taatgtgcct tgatgtatgc ggtttggtag gccagaaagg catagcaggt ggaatgtgac
     4621 ttgttgaagg catattcggc aaaggcttcc cagttggtcc agattttttc cactttagcc
     4681 tggtcgtgtc cgttgttgat gcatccatgg ataaattttc ccttcatttt gtccatgacg
     4741 tcccggagtt ttttccccat tgcttttcgc agagaatcag cctcacccct tgtaaatccg
     4801 gcaagtttct gcgagagtag catcacctgc tcctggtaaa cggttattcc ataggtatcc
     4861 tttaggaatt cctccatttc gggtatgtcg tactctattt tctcccgacc atgcttccgg
     4921 ttgatgaagc tggggatata ctccataggc cccgggcggt agagcgcatt catggcaatg
     4981 aggtcctcga atcggtttgg ctccaaggct ttaaggtatt ttttcattcc ggccgactca
     5041 aactggaaaa tggcggtggt atcgccccga gagaagagtt cgagtgttgt cgaatcatcc
     5101 aaggggatat tgtctatatc gagctgtt
//
LOCUS       DAOOQX010000050         5075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_19808_length_5075_cov_2.791235, whole genome shotgun sequence.
ACCESSION   DAOOQX010000050 DAOOQX010000000
VERSION     DAOOQX010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19808_length_5075_cov_2.791235"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(306..1010)
                     /locus_tag="PLX53_01465"
     CDS             complement(306..1010)
                     /locus_tag="PLX53_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71779.1"
                     /translation="MKRITAIMVVFLLAALGLADQAQAQRYRKKEISLISFKLYVDPD
                     YRAKLESYSNLFPEPQASSKVNKIDNLILYTSWDIIKRRLEGEVGMIILPIDAYGDKF
                     SYDMYGFPDISINKALSKGRSKYYIKLDLELVPEHSAKYTTQNHVDSARKIVQLKEDE
                     IKPKVVAKLTIYSDKGVIPVANCVGEATTKSIITLSSSFFDGVVNSSIDPSAETLYNL
                     IDKASQQLIISVYENK"
     gene            complement(1181..4405)
                     /locus_tag="PLX53_01470"
     CDS             complement(1181..4405)
                     /locus_tag="PLX53_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOY71780.1"
                     /translation="MSRLVVYRASAGSGKTFRLAAEYIRMVVTNPYAYRIILAVTFTN
                     KATTEMKERILSELNQLAGGASTPMLEVIISESKLSETTIRQNAKSVLHAILHDYSMF
                     SVSTIDSFVQRVIQNLLWEVGFHSLGELMLEHDAILDRAVDRLLDSTNGDSELFTHLK
                     SMIFYQLDQGKSPDIKKSLVELGNLLYSEQYRLLTDEERAKMGDKSLLVNITTQATER
                     LNAIVQTICSMGKALMQEISSNGFTVDDFKNKSGGVYGFIEKTANFSLGFNYIEVGER
                     ASVASQDYTGDQWMSREFVKANPGKSSELQHLVQQSLQPGLARLVEYIEDNRSMFNTT
                     TALRSNLNRLYIVNEIRRYIKEIQAEDNVMVLADSGPLLREFIGENDAPFVYEKIGTR
                     YDSFMLDEFQDTSVIQWQNFKPLVGNSLAQDGFSMVVGDVKQAIYRWRNSDWRILASQ
                     LEEDFEVEPRNLSVNFRSLPGIVNFNNQFFGKAATWLKGWVNRLFEDNPPEDLLKVVE
                     KLYGGAAQESKFHEAVGGLVEVSCFQNIKAGRYDEFLKRKFSMLIPDILKRGYRPGDV
                     AILVRKHSEGKKIADMLLSLKNENPEWADSFNLVSQDALLLDSSHAIRLFISALKLIL
                     NTNDGVSRGVFCKESMVVAKISTSWEHTFVGSDIDDEIARLTGLRNLPLSTIFEEVLL
                     RYNLQELKQELPYIALLNEQILNYSHGNLASVAGFLEWWDDRGHEVSLSLPDSADAIN
                     ILTFHKSKGLEFPVVIIPYASNSIFKNDKEKALWVSSATEPYCDYPKFLIGMADYLKD
                     SDFRDDYLAEKFQTMVDNLNLLYVAFTRPQNELYVMLSHQADDPKTVQDTGDIILSIV
                     KDMDAPEFTDDRFADISALAVYQFGLVAPIPKTSESKVENRPDVWLMEQYPVGKKIPS
                     LAGHLEASDFFKDEELTVTSGVEHGRIMHRLFSMIRGIGDVSMAVSRLVAEGLISLQQ
                     APALEQHVSEAIQNDAVADWFSEKWQELKVEASILTPTGQTYRPDRVMVSNGHAVVVD
                     FKFGEQQNAHKKQVAGYSELLSQMGYPNVTGYLWYFDSGSIVRV"
     gene            complement(4679..>5075)
                     /locus_tag="PLX53_01475"
     CDS             complement(4679..>5075)
                     /locus_tag="PLX53_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71781.1"
                     /translation="LEVEAINEKLATEGKNFILIGPGRWGTRDRWIGIPVTWPQISNA
                     KVIVETSLDDFPLDASSGSHFFHNVISMNVGYCSVPNNDSFARIAWSKLDELPAYNET
                     KYFRHVRFPKPLQVKMDGTQRLIVVTHEP"
BASE COUNT         1361 a         1212 c         1100 g         1402 t
ORIGIN      
        1 gtgaaacact cgatttgatt gaaagtcata aactgattat tgagagtcgt aggctttcac
       61 ctgaaatcaa agccatgttt gcttctatga gaaacagatg ttatttggtg gcgcaagctt
      121 ttcaatatca cttagcttga tttttacttc agcactataa gggacagtat tggctgcttc
      181 aatggtttag gcccacgaat ctgacttttt ttcgaaatga gctaggcaaa tatccaaacc
      241 atcgagctag gttccagaca ttacaccaac tacgctcaac ggatgtttgt tgaagagctt
      301 taccatcact tgttctcgta aacgctgatg atcagctgct gcgaggcctt atcaatcaag
      361 ttgtatagcg tttcagcaga aggatcgatg ctgctgttca ccacgccgtc gaaaaacgag
      421 ctgctaaggg taataatgct ttttgttgta gcctccccaa cacaatttgc caccggtata
      481 actcccttat cggagtatat ggtaagcttt gcaactactt ttggtttaat ctcatcctct
      541 ttaagctgaa ctattttcct agcagaatca acgtgatttt gggtggtgta cttagcagag
      601 tgctccggaa ctaattccaa atctagctta atatagtact ttgatcttcc ctttgaaagg
      661 gctttattta tactaatatc cggaaatccg tacatatcgt aggaaaactt atcaccatag
      721 gcatctatgg gaagtataat catacctacc tcaccctcga gccttctttt tattatatcc
      781 cacgaagtgt aaagtataag gttgtcaatc ttgtttacct tactgctagc ctgaggctcg
      841 gggaacaggt tgctataact ttcaagtttt gcccgatagt cggggtcaac gtatagcttg
      901 aatgagatta gcgaaatttc ctttttgcgg tatcgctgcg cttgcgcctg gtcagccaaa
      961 cctagagctg ccagtaaaaa cacgaccatt atagctgtaa ttcttttcat cccccaatca
     1021 atttataaat tccggaaaaa actctacgag caaagatagt caatttttca aacgagctcc
     1081 gggttcaata aattgtactt ttgctacaaa tttcacgttt gtagcggggt tctcaattgc
     1141 ttcagcataa aagtggcagt gagctgccag cagttagctt ttacacccgg actatggaac
     1201 cggaatcgaa gtaccaaagg taacctgtca cattggggta acccatttgc gacaggagtt
     1261 ccgaataccc tgccacctgc ttcttgtggg cattctgctg ctcgccaaat ttaaagtcaa
     1321 ctaccacggc atggccattg ctaaccatca cccggtcggg gcgataggtt tgcccggttg
     1381 gggtgaggat ggaagcctca actttcaact cttgccattt ttctgaaaac cagtcagcta
     1441 cagcatcatt ctgaatagct tcgctgacat gctgttccaa cgcaggagct tgctggagag
     1501 aaatcaggcc ttctgcaaca agtcggctga cggccatgct cacatcacct attcccctga
     1561 tcattgaaaa caagcggtgc ataattctcc cgtgctctac tcctgaggta acggttaatt
     1621 cttcatcctt aaaaaaatcg gatgcctcaa ggtgtcctgc cagggaagga attttttttc
     1681 caacagggta ctgctccatc agccaaacat cggggcgatt ctctacctta ctctcggatg
     1741 tttttggtat tggagcaacc agcccaaact ggtaaacagc taatgctgaa atatcggcaa
     1801 acctgtcatc ggtaaattca ggggcatcca tatctttaac aatggaaaga atgatgtctc
     1861 cggtatcctg caccgttttg gggtcatcag cctggtggct aagcatgacg taaagttcgt
     1921 tctgaggtct tgtaaaggct acgtagagca ggtttaggtt atccaccatg gtttgaaact
     1981 tctcggcaag gtagtcgtct ctgaaatcgc tatccttaag gtagtcggcc atgcctatca
     2041 ggaatttcgg gtaatcgcag tatggttcag tagcagacga cacccataat gctttctcct
     2101 tgtcgttctt aaagatgcta ttgctggcgt aggggataat cacaaccgga aactcaagcc
     2161 ccttggactt atgaaaagtt aaaatgttga ttgcatcagc cgagtcggga agcgagaggg
     2221 aaacctcgtg tccacggtca tcccaccact ccaggaagcc agcaactgaa gccaagttcc
     2281 cgtgagagta gttcaaaatc tgctcgttga gcagggctat atagggcaac tcctgcttca
     2341 actcctgcaa gttatagcga agtaaaacct cctcaaaaat ggttgaaagt ggaagatttc
     2401 taaggccagt aagcctggca atctcgtcat caatatcgct tccaacgaag gtatgctccc
     2461 agctggttga aattttagct accaccatgc tctccttgca gaaaacgccc cttgacacgc
     2521 catcgttggt gtttaatata agcttaaggg ccgaaataaa gagcctaata gcatgtgatg
     2581 agtcgagtag cagggcatcc tggcttacca ggttaaagga gtccgcccat tcaggatttt
     2641 cgtttttgag cgatagcaac atgtcggcaa tctttttgcc ctcgctgtgt ttcctaacca
     2701 ggatggctac atctccgggt ctgtacccac gtttcaagat gtcgggtatc agcattgaga
     2761 acttgcgttt tagaaactca tcatacctac ctgctttaat attctgaaag cacgatacct
     2821 ccacaagtcc acctacggct tcatgaaatt tgctttcctg tgctgcacct ccatacagct
     2881 tttcgactac tttcaaaagg tcttcgggag gattatcctc aaagagccga ttaacccaac
     2941 ccttcaacca tgttgcagct ttaccaaaga attggttgtt aaagttcaca atcccgggaa
     3001 gactgcgaaa gtttacggac aaattgcggg gttccacctc aaagtcttcc tcaagctgcg
     3061 aggccagtat tcgccaatcg ctgttacgcc agcggtaaat tgcctgcttc acatcgccaa
     3121 ccaccatgct aaaaccatcc tgagccaagc tattgccaac cagaggttta aagttttgcc
     3181 actgaataac cgaggtatcc tgaaactcat cgagcataaa gctatcgtag cgggtaccga
     3241 ttttctcata aacaaacggt gcgtcgttct ccccaataaa ctcacgaagc aatggtccgc
     3301 tatcggctag caccatgacg ttatcctctg cctgaatctc cttgatgtac cttcgtatct
     3361 cattgactat gtagagccta ttcagattgg aacgtagcgc cgtggttgtg ttgaacattg
     3421 agcggttgtc ctctatgtac tcaaccagcc tggccaaccc gggctgtaac gattgttgaa
     3481 ctaagtgttg caactccgat gatttgcctg gatttgcctt gacgaactcc ctgctcatcc
     3541 attgatcgcc agtgtaatct tgtgaggcta cacttgccct ttcaccaact tcaatgtaat
     3601 taaaccccaa actaaaattt gcggttttct ctataaatcc ataaacgccg cccgacttat
     3661 tcttaaaatc atcaacagta aagccgttgc tgctgatctc ctgcatcaag gccttgccca
     3721 tgctgcaaat tgtttgaact atggcattta acctctccgt ggcctgagtg gtaatgttga
     3781 ccagcaacga tttatcgccc attttggctc tttcctcgtc ggtaagcaac cggtactgct
     3841 cggagtaaag caggttgccc agctctacca aacttttctt aatatcaggg ctttttccct
     3901 gatcgagctg atagaagatc atggatttta agtgagtaaa tagctctgaa tctccgttag
     3961 tgctgtcgag cagcctgtca acagccctgt cgagaatagc atcatgctca agcatgagct
     4021 cgcccaagct atgaaagcct acctcccaga gcaggttttg aatgacacgc tgaacaaagc
     4081 tgtcgatggt gctaacgctg aacatggagt agtcgtggag gatagcatgc agaaccgatt
     4141 tggcattttg gcgtatggtg gtttcggaca atttagattc tgaaataata acctcgagca
     4201 tcggggtgct ggcaccacca gccagctggt tcaactccga aagtatgcgc tccttcattt
     4261 cggtggttgc cttgttggta aatgttaccg ctagtataat cctgtaggca taagggttag
     4321 ttaccaccat gcggatgtac tcggcagcca gcctgaaagt ctttccagaa ccagcggatg
     4381 ccctataaac aacaagccta ctcatagcaa cagctaaaaa tcaatcaact ctaaaaatag
     4441 gaaaaatctg aataaatcca cagggtgggc agaatgttgc tggaattata agtgggtgtt
     4501 aaaggataaa ggttaaagga taaaaaaatt caagcaggat tcaagaacct gtccgcctac
     4561 tggcggaact tgtccggctc ctgacggaac ttgtccgcct accggcggac aagttcctga
     4621 atctggaatt atttttttct ttaactgtag aagcttccag ctagacgctc cagcatactc
     4681 agggttcatg agtaaccaca atcaaacgtt gcgtgccatc cattttaacc tgcagaggtt
     4741 taggaaagcg gacatgcctg aaatatttag tttcgttgta agcaggtagc tcatcgagct
     4801 tactccatgc aattcgtgcg aagctatcgt tgttaggaac agagcagtat cccacgttca
     4861 tggaaataac attgtgaaaa aagtgggagc cggatgaggc atcgagggga aaatcatcca
     4921 agctagtctc gacaattact ttggcattgg aaatttgtgg ccaagtaacg ggaatgccaa
     4981 tccacctatc gcgggttccc caccttccgg ggccaatgag aatgaaattt tttccctcag
     5041 tagccagctt ttcgttaata gcctcaacct ctaaa
//
LOCUS       DAOOQX010000051         5040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_19964_length_5040_cov_4.518957, whole genome shotgun sequence.
ACCESSION   DAOOQX010000051 DAOOQX010000000
VERSION     DAOOQX010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19964_length_5040_cov_4.518957"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLX53_01480"
     CDS             <1..455
                     /locus_tag="PLX53_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOY71782.1"
                     /translation="VFRNEAISSRAHCIFHQVEGLYIDKTVSFADLKQTLEFFVKEMF
                     GSKTSIRLRPSYFPFTEPSAELDISCNLCGGKGCSVCKHTGWLEILGCGMVDPNVLEL
                     NNIDSKKYSGFAFGMGIERIAMLKFRVNDLRNYFENDIRFLNQFTNFY"
     gene            544..1185
                     /locus_tag="PLX53_01485"
     CDS             544..1185
                     /locus_tag="PLX53_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOY71783.1"
                     /translation="MTTEESEQLEANLECYEYKKGEIIYKEGSRITGFYCVKKGVFKI
                     YKTGIDSKPQIVAFAQKGDITGYRSVLSNEPACTTVESLEDSEVCYIPSSLIFDFIRK
                     NSNFALSLIQLTCKELNTANDLIKDIAQKTVRERLAETLLMLDQTFGKDEEGFINVNL
                     TREDISSIIGTATESVIRILSEFKTDGLISLKGKKIGIGNYSKLESISNSFLK"
     gene            complement(1177..2172)
                     /gene="ruvB"
                     /locus_tag="PLX53_01490"
     CDS             complement(1177..2172)
                     /gene="ruvB"
                     /locus_tag="PLX53_01490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOY71784.1"
                     /translation="MENQISDKEFENNIRPSEFKQFNGQDDIIKNLKIFVQAAKIRSE
                     SLDHVLLHGPPGLGKTTLAYIIANELGVSIKTTSGPVLEKPGDLAGILTNLDKFDILF
                     IDEIHRLSPIVEEYLYSAMEDFRIDILIDKGPSARSIQIELNPFTLVGATTRSGLLTS
                     PLRARFGINFHLEYYDASTIKSIIKRSSYILNIDIDDDASMEIALRSRGTPRIANSLL
                     RRVRDFAQVVGNGRINLDIAKSSLDALKIDSLGLDEMDNKILTTIINKFNGGPVGINT
                     IATAISEDPGTIEEVYEPFLIKEGLLKRTPRGRQTTDLAYSHLKIEKNFRQGTLF"
     gene            complement(2181..3161)
                     /gene="nadA"
                     /locus_tag="PLX53_01495"
     CDS             complement(2181..3161)
                     /gene="nadA"
                     /locus_tag="PLX53_01495"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOY71785.1"
                     /translation="MNYSIVDKLGYIDEDIDSNIDLKVAIRKIKEEKNAVILAHYYQR
                     PEVQDIADFVGDSLALSLKAASLNSSLILFAGVKFMAETVKILRPNSQVLIPDLNAGC
                     SLADSCQFSDFKNFIDLHPNHIVVTYVNSSAEVKTISDICCTSSNAIKVVKSLNNSTP
                     LIFAPDINLGSYVKSTLNRSNMVLWNGSCHIHNQFILQDIITLKTKHPKAKVIAHPEC
                     QNSILLISDFIGSTKSLLDFTYNSNNTDFIVVTEPGIIHQMKKLSPQKNFYFPYVQNE
                     ECYCNECSYMKLNNLKKIYLTLKYEYPIINLEPDVISNAAIPINRMLKIN"
     gene            complement(3693..>5040)
                     /locus_tag="PLX53_01500"
     CDS             complement(3693..>5040)
                     /locus_tag="PLX53_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOY71786.1"
                     /translation="RKKVKSGIVLILGNTESPINYQGNTYNFRQDSNFLYFFGLDTPG
                     LAGVIDIEEGTDCLYGDDFDIDDIIWMGPQPKLKDMGEKVGVLSTHPFRELSTTLNRA
                     IRQGRKIHFLPPYRADNTLLLEKLLGINQTIIKTYSSIELIKAIVSLRSVKEPCEVEE
                     IEKACSVGYKMHVTAMKMAKPGALEREIAGELEGIAHSFGYMTSFPTILSQHGETLHN
                     HDHSLTLEEGRLLLVDAGAEGISHYASDFTRTFPVGGHFSQKQKDIYSIVLAANNKTA
                     ELAKPGVTYFNVHKAAARVIVEGLSALGIMKGNSDEAVENGAHALFMPHGLGHMMGLD
                     VHDMEDLGEDFVGYDDEISRSEQFGTSSLRMARKLQKGFVLTNEPGIYFIPALIEKWK
                     SEKINQAFINYDVLSSYLTFGGIRLEDDLLITDNGCRILGHRIPITPEEVEKTMNS"
BASE COUNT         1651 a          853 c          962 g         1574 t
ORIGIN      
        1 gagtatttag aaatgaggca atttcatcaa gagcacactg catatttcat caggtagaag
       61 gtctgtatat agataaaacc gtatcgtttg ccgatttgaa acaaacacta gagttctttg
      121 ttaaagaaat gtttggaagt aaaaccagta taagattgag gccatcatat ttcccattta
      181 cagaaccttc agcagaatta gatataagct gcaatttatg tggtggtaaa ggatgttctg
      241 tatgtaaaca tacaggatgg ctcgaaattt tgggttgtgg aatggtcgat ccgaatgtgt
      301 tggaacttaa taacattgat agtaaaaaat attcaggttt tgcatttggt atgggaattg
      361 agcgaatagc catgttaaaa tttagggtta atgacttaag aaattatttt gaaaatgata
      421 taaggttctt aaatcaattt acgaattttt actgattttc tataggcaaa aatttggtag
      481 gtaatgaaaa gcatgaacgt ttgcaaaacc tgtagcctgt cgtcgaaaac ggtttttggt
      541 tctttgacta ccgaagaatc agaacaacta gaagcaaatt tggaatgtta cgaatacaaa
      601 aagggagaaa taatttataa agaaggtagc agaattacag ggttttactg tgttaagaaa
      661 ggagtattta aaatttataa gaccggaata gactcaaaac ctcaaatagt agcttttgct
      721 caaaaaggcg atataacggg ctacagatca gttttgagta atgaaccagc atgtactact
      781 gtggaatcac tagaagatag cgaagtatgc tacataccat ccagcttgat atttgatttt
      841 attaggaaaa attcaaattt tgccttatca ctaatccagc taacctgtaa ggaattgaat
      901 actgccaatg acctgataaa agatatagct caaaaaacag taagggaaag gctagcagaa
      961 accctactaa tgcttgatca aacatttggt aaggatgagg agggatttat aaatgttaac
     1021 ctcaccagag aggatatttc aagcataata ggaacggcaa cagaatcagt tataagaata
     1081 ctaagcgagt ttaaaacaga tgggctaata agcttaaagg gtaaaaaaat aggaattggt
     1141 aattattcta aactagaaag tatttctaat tcatttttaa aatagggttc cctgcctaaa
     1201 atttttttca attttcaaat gggaatatgc taaatctgtc gtctgtcttc ctcttggagt
     1261 acgctttagt aaaccttcct taatcagaaa aggttcataa acttcttcaa tagttccagg
     1321 gtcttcgcta atagcagttg cgatagtatt tattcctaca ggtccaccat taaatttatt
     1381 gattatggtt gttaatatct tattatccat ttcatcaaga cctaacgaat caatttttaa
     1441 agcatctaaa gaactttttg ctatatcaag attaattcgg ccgtttccta ccacctgagc
     1501 aaaatcgcga actctacgta gtaaactgtt ggctattcgg ggagttcctc tactcctaag
     1561 tgcaatttcc atagaagcat catcatctat gtcgatattc aaaatataag aggatcgttt
     1621 aatgatagat tttatagtac tagcgtcata gtactccaga tgaaaattaa ttccaaaccg
     1681 tgctctaaga ggactagtaa gtaaaccgct tcgagtagtg gcaccgacaa gagtaaatgg
     1741 attcaattca atttggatgc ttctggcact tggaccctta tctattaaaa tatctattct
     1801 aaaatcctcc atggcagagt aaaggtattc ttctactatt gggctcaaac gatgaatttc
     1861 atcaatgaaa agtatatcga atttatctaa atttgtaagt attccggcca aatcgccagg
     1921 cttttcaaga acaggtcctg atgtagtttt tatagaaact ccaagttcat tcgctataat
     1981 ataagccaga gtagtttttc cgagtccagg aggaccatgt agcagcacat ggtctaatga
     2041 ttcagaccta attttggcag cttgtacgaa aattttaagg ttttttatta tatcatcctg
     2101 accattaaat tgtttgaatt cggatggcct aatattgttt tcaaattctt tgtcggaaat
     2161 ttgattttcc atatgtttaa ctagttaatt tttaacattc gattaatagg aatagctgca
     2221 ttagaaataa catcgggttc taaatttata attggatatt catattttaa agttaagtaa
     2281 atttttttta agttatttaa tttcatatag ctacattcat tacaataaca ttcttcattt
     2341 tgaacatagg ggaaatagaa atttttttgt ggtgataatt tcttcatctg gtgaattata
     2401 cctggttcag taacaacaat aaaatcagta ttatttgaat tgtatgtaaa atcaaggagg
     2461 cttttagttg aaccaataaa atcagaaata agtaaaattg aattttggca ttccggatgc
     2521 gctataactt tagccttcgg atgtttagtt ttaagagtaa taatatcctg taatataaac
     2581 tgattatgaa tatggcaaga accgttccat agaaccatat tagatctgtt tagtgtagat
     2641 tttacgtagc tgcctaaatt tatatcaggt gcaaaaataa gcggggtaga gttatttaac
     2701 gatttaacaa cctttatagc attagatgaa gtgcaacaaa tgtcagaaat cgtttttacc
     2761 tcagctgaag agttaacata ggtaactaca atatgatttg ggtgtaaatc gataaaattt
     2821 ttaaaatcag aaaattgaca tgaatcagca agactacaac cagcatttag atcaggtatt
     2881 agaacttgtg aatttggtcg taaaattttt acagtctcag ccataaattt cactcctgca
     2941 aacaaaatta agctactatt aagacttgca gcttttaaag aaagggctag gctatcacca
     3001 acgaaatcgg caatatcctg aacttcgggt cgttggtagt agtgcgcaag aataaccgca
     3061 tttttttcct ccttgatttt gcgtatagca actttcaagt caatgttgct atcaatatct
     3121 tcatcaatat accctaactt atcaacaata gaataattca ttaatattta tttaagtatt
     3181 aaaaaaaaaa tttaaattat gattatttgt tgtttatatt gttggtaaaa aacggaaaac
     3241 aataatgcaa aattcggtat taaaaggcaa ataacaaatt attaacacaa agaaaagatg
     3301 gttattgctg gtaatcagca aataaaagaa gtaattaaca tcgggtaacg ccgggcaatg
     3361 ttaaaacatt ttccgaatta taccacccga tttagttagc taagctgttt aaaacttcag
     3421 gtatagattt gtaagatact cttgacacgt atcgagaatt tattaagtag ttaaattgga
     3481 atgcatgaca acaaaaaagc attaatagtt ttcaacagtc aataataaag aacttaacag
     3541 taaacggtaa gatgttaact ggatttttac cgtaatgagc attacggtag tggaggttac
     3601 atgcaaaact acgtccgcta tctatttatg aaacaaaaaa ggagcgtgga tgttgccaac
     3661 gctcctttcg aatcggctat gcagcaattt tgctatgaat tcattgtttt ttcaacctct
     3721 tcgggggtaa tagggattcg gtggcctaga atacggcatc cgttatcggt aattagtaaa
     3781 tcgtcttcca ggcgaatgcc tccaaaggtt agatagctgg acagtacatc gtagttgata
     3841 aatgcctgat taatcttttc ggatttccat ttttcgataa gggcaggaat aaagtagatg
     3901 ccgggttcgt tagtcaggac aaagcccttc tgaagcttgc gggccatgcg aagtgaagaa
     3961 gttccaaatt gctcggagcg tgaaatttcg tcatcgtagc caacaaagtc ttcgccgagg
     4021 tcctccatat catgaacatc aagccccatc atgtgtccta atccgtgggg cataaaaagt
     4081 gcgtgagcgc cattttctac agcctcgtcg gaattcccct tcataattcc aagagcagaa
     4141 agaccctcaa caataaccct tgctgcagcc ttatgcacgt taaagtaagt aacgccaggc
     4201 tttgctagtt ctgcagtttt attgttggca gccagcacta tagaataaat atccttttgc
     4261 ttttgtgaaa aatgcccgcc aaccggaaaa gttctggtga aatcggaagc gtaatggcta
     4321 atcccctcgg ctccggcatc aaccagtaat aatcgtcctt cctcaagggt aagtgagtga
     4381 tcgtggttgt gaagagtttc accatgctgg gagagaattg ttgggaaaga ggtcatatat
     4441 ccgaaagagt gagctattcc ttcaagttcg ccagcaattt cgcgctccaa agcgccgggc
     4501 tttgccattt tcatggcggt aacatgcatt ttgtaaccca cagagcacgc cttttctatt
     4561 tcttcaacct cgcagggttc ttttactgag cgaaggctaa caatggcttt tatgagttca
     4621 atggaggagt aggttttaat aatggtttgg ttaattccca ataatttttc caggaggagg
     4681 gtattatctg ccctgtatgg cgggaggaaa tgaatttttc ggccctgcct tatggctctg
     4741 ttcaaggtag tagagagttc tctaaatgga tgggtagaaa gaacgcctac cttttcaccc
     4801 atatccttca actttggctg gggtcccatc caaataatgt cgtcaatgtc aaaatcatcg
     4861 ccgtaaaggc aatcggtacc ctcttcaatg tcaattaccc cagcaagccc cggggtgtca
     4921 agtccaaaaa agtaaagaaa attactatcc tgccgaaagt tatacgtgtt accctggtag
     4981 ttgattgggc tttcggtatt tcccaaaata agaactatgc cagattttac cttttttcgc
//
LOCUS       DAOOQX010000052         5033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_20009_length_5033_cov_2.981720, whole genome shotgun sequence.
ACCESSION   DAOOQX010000052 DAOOQX010000000
VERSION     DAOOQX010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20009_length_5033_cov_2.981720"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            331..2832
                     /locus_tag="PLX53_01505"
     CDS             331..2832
                     /locus_tag="PLX53_01505"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY71787.1"
                     /translation="MPEKQDKTKVSAAFYFFKAGLLLLLMVTVMVVKGQTGQLLVVGF
                     VTDSVGSPVPFASVVVEGGPIGVSTDINGRFRITLPAAQSAVLTISCVGYKSTSVLIN
                     ADVNSPIVELRVKLDVNELLIDEIRVSSDRRWTGNIERVNARDISFMPNVSGNFESII
                     KLMPGVASTNELSSQYSVRGGNFDENLVYVNDIEIYRPFLVRSGQQEGLSFINSDLVS
                     AVEFSAGAFNAEFGDRMSSVLNVSYRMPTSYQSGVSLSLLGASAYTEGVLGNRKFSYI
                     TGVRYKTSSYLLNTLDVKGDYTPSFLDWQGLFSYRLNDKLAFSFLGNYATNSYQFAPD
                     VRETRFGVFSNALQLKIYYEGKELDRYISGMGAAYVEYAPSQNTSIKIYSANYLTHES
                     ETFDLLGEYFLNELDNSMGSGSYGDSLINVGIGGFLNHARNFLFARINNFGLVGSVRF
                     SNAKLKWGAQLQHEFFDDELNEWNLLDSSGYAIPYNGSAIGLSNVMVAGNSTSVNKLS
                     AFSQLDYSLSRGAFTLATNTGLRLLYWDYTGDVLVSPRVSLSLKNQRLKNFNFHLAGG
                     VYYQPPFYRELRMPNGSLNPNIKAQRSVQLLAGLEYYFTAWNRPFKLSVELYNKWLDN
                     LIPYKVDNVRIRYAGQNLAKGFARGVDIKLNGELLEDAESWVSISLLKTMEDILHDSY
                     VDANGQTVYPGYYPRPTDQTLGFNLFFQDYLPGNPTFRVHLSANYGTGLPVGVNGIDR
                     YDVTFRMKDYKRVDIGFTKILRDEISSNGISRKLKGFKGIWLGAEVFNLFNFNNTVSY
                     LWIQTVGNQQGESDTFAVPNYLTSRRLNIRLQLKF"
     gene            2964..>5033
                     /locus_tag="PLX53_01510"
     CDS             2964..>5033
                     /locus_tag="PLX53_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOY71788.1"
                     /translation="MNGNSLSSKESETHIYLQHIHRYLRAGFWLWNSETNHLLLGHKF
                     QEALGVKADELGIHLDDFFSVVHPDDRRQLSTILFRLLKQKLRYREFDVRVTVNDVWH
                     RLRIHALVGEIATDGSVQSLFGKFLPADPRQPISGPAIPASTFMGFDAFTYNPEEGQL
                     QWQFQPSSMFWGNTPKGKTWNFSEFRAILSPSSFKELSQSWQVFANGNEEFFTRNLTL
                     SSPQNAKRIITLLARKVEGSRYIQGVMIDTSKLCGIADKSSFLHFYSSIASSHRLLMI
                     ELSREGAIKGCNRITEKSFGYSLAELQNPQLLPSLFTNADSFKQLVTYANSGGSKPFV
                     CRVRNREGQVKDISWILLKLPEVVGMEGNTLIGQDVTRQVQVNFEVNLLQQRYQAIKL
                     ANAELQGAANFEGVFSCFGQQLERIFPSMVTVVLSYNAADGFITVEGIFGIQHKTWES
                     LIFDLGWNPVGRRFLLHDKDKHAIKSAAAYRVNESFTGIMEGYISMSASKIIERYINI
                     EAIYAEGLVSGNHMYGGVLLLKPEPADEPDLTLLEELASVAVNTIQRIDTAEGLQQQI
                     FTLKSKIEQKDELFSQLSHQLRTPLNAVLGFSQLLLELNQPDDLLKKYIGIINSKGKQ
                     LLRLVNDTIDYNLIEKGNLTLVKSTFNLNHLLQQLHRSFSQQFELYQNEGIKLKFIQP
                     GNAENIFL"
BASE COUNT         1372 a         1063 c         1167 g         1431 t
ORIGIN      
        1 ttaatgaata tgttggccat tccggtttcg ggtaacgccg gcaatttttt aacaaccaac
       61 tcggttatca ggtgaaaacc aattggaaat ttggggaggg caaattcaac ctgttgaatc
      121 atgctcaggc attttatgtt tcacaattag gtaaatcgaa agcgggagta aatttaaaaa
      181 tcaactaaag gtaaggaaat ttgatttgta aaccatgttg acagctattg tagcacaatt
      241 tttttaagca aatatggtta ttcttaggct tggtattgtt cagcatgcta cttttgaggg
      301 aatatgtaca gataaactca acaacttgta atgcccgaaa aacaggataa gactaaggtt
      361 tccgctgctt tctatttctt taaagcaggg ttgctcctgt tgttaatggt aaccgttatg
      421 gttgtaaaag gccagacggg tcagctcctg gttgtagggt ttgtaaccga ctcggttggc
      481 agccctgttc cttttgccag cgttgttgta gagggtgggc caataggggt ttcgaccgat
      541 atcaatggac ggtttcgcat tacccttccg gctgcgcaaa gtgctgttct caccatttcg
      601 tgtgtaggct acaaaagcac ttcggttctt attaatgcag acgtaaatag cccaattgtt
      661 gagcttaggg tgaagctaga tgttaacgag ttgctgattg atgagattcg tgtatcgtcc
      721 gaccgtcgct ggacgggtaa catcgaaagg gttaacgcca gggatattag ctttatgccc
      781 aatgtttctg gaaattttga atccattatc aagctgatgc cgggcgtggc atcaaccaac
      841 gagcttagct cgcagtactc ggttaggggg ggaaattttg atgagaactt ggtttacgtt
      901 aacgatattg aaatttacag gccattttta gtaagatcgg ggcagcagga ggggttaagc
      961 ttcataaatt ccgatttggt ctcggctgta gagttttctg ccggagcatt caacgccgag
     1021 tttggcgaca ggatgtcgtc ggttctgaat gtgagctacc gaatgccgac ctcctaccag
     1081 tctggtgtgt ctctaagtct gcttggtgcc tctgcataca ccgagggtgt tttgggtaac
     1141 cggaagtttt cgtacataac cggagtgcgc tacaaaactt caagctacct gctgaacacg
     1201 cttgacgtaa agggcgacta caccccttca ttcctcgatt ggcaggggtt gttttcgtat
     1261 aggttgaatg ataagcttgc gtttagcttt ctgggcaatt atgccaccaa cagctaccag
     1321 tttgctccag atgttagaga aaccaggttt ggcgtgttca gcaatgcgct tcagctgaaa
     1381 atatactacg agggtaagga gctggacagg tacatttcgg gaatgggagc cgcttatgtg
     1441 gagtatgccc caagccaaaa caccagcata aagatttact ctgcaaacta ccttactcac
     1501 gagtccgaaa cattcgacct gcttggagaa tacttcctga atgagcttga caactcaatg
     1561 ggatcgggtt cctatggcga cagtctgatt aacgtaggaa tcggtggatt tctgaatcat
     1621 gcccgcaact tcctctttgc ccgcatcaat aattttgggc ttgttggaag tgtgaggttt
     1681 tcaaatgcca agctaaaatg gggcgcccag cttcagcatg agttcttcga cgatgaactg
     1741 aacgagtgga atctgcttga ctcctcaggc tatgccattc cctataatgg ttcagccatt
     1801 ggcctttcaa atgtgatggt agcaggtaac tcaacctcgg tgaataagtt atcggctttt
     1861 tcacagctcg attacagctt aagcagaggg gcatttaccc ttgccacaaa taccggacta
     1921 cgtcttctct actgggatta taccggtgat gtgctggtaa gtccccgagt ctcgttatcg
     1981 cttaaaaatc aaagacttaa aaattttaac ttccatttag cgggtggcgt ttactaccaa
     2041 cctccttttt accgggagct gcgaatgccc aacggaagct taaatccaaa tatcaaagct
     2101 caacgttcgg tgcagctgct tgctggtttg gaatactact ttactgcctg gaaccgacct
     2161 tttaagctgt cggtagaact ttacaacaaa tggctcgaca accttatccc ctataaagtt
     2221 gacaacgtgc gaattcgtta tgctggacaa aacctggcta agggttttgc ccggggcgtt
     2281 gatattaagc ttaatggtga gctgcttgag gatgccgagt cgtgggttag catttcgcta
     2341 cttaaaacca tggaggatat tcttcacgat agctatgttg acgccaacgg acaaacggtt
     2401 tacccagggt actatccccg accaaccgat caaaccctgg ggtttaactt gttcttccag
     2461 gattacctac ccggtaatcc gactttcagg gtacacctta gcgctaacta cgggacaggc
     2521 ttgcctgttg gtgtgaacgg tatcgacagg tatgatgtta ccttcagaat gaaggattac
     2581 aaaagggttg acatcggttt taccaagata ctccgtgacg aaatctcttc taatggtata
     2641 tcccggaagt tgaaaggatt taaggggata tggttgggcg ccgaggtttt caacctgttt
     2701 aatttcaaca acacggtttc ctacctctgg attcaaaccg ttggcaacca gcagggtgaa
     2761 tcagatacct ttgctgttcc caactaccta acctcaaggc gcttaaatat caggttgcag
     2821 cttaaattct aaccaatagc ttttgttttc ctaaaaaaat gttgagagtt tagcctaact
     2881 ttgctatttt tataaattgt aaagtaaaaa gctaaattgg ttcatcattg tagcatactg
     2941 ttaaaggatt ttcctctgta aaaatgaatg gaaatagctt atcaagcaaa gagtctgaaa
     3001 cacatatcta cctacagcat atccatcgtt acctaagggc tggattctgg ctgtggaatt
     3061 cagaaaccaa ccacttgctg ctagggcata aattccaaga ggcacttgga gttaaggctg
     3121 atgagctggg catccatctt gacgacttct ttagcgtagt tcaccccgac gatagaaggc
     3181 aactttctac tatccttttc cgattgctga agcaaaagtt acgctacagg gaatttgatg
     3241 ttagggtaac ggtgaacgac gtatggcata ggttgagaat tcatgcttta gtaggagaaa
     3301 tagctaccga cgggagtgtg caaagcctgt ttggaaaatt tttacctgct gatcccaggc
     3361 aacccatatc cgggccagcc atacctgcat caacatttat gggctttgat gcttttacct
     3421 ataacccgga ggaaggccag ctgcaatggc aatttcaacc tagttctatg ttttggggaa
     3481 atacaccaaa aggtaaaacc tggaattttt cagaatttag ggcaattctt tcaccctcca
     3541 gcttcaagga gctctcgcaa agctggcagg tttttgccaa cggaaatgaa gaatttttta
     3601 cacggaactt aaccctttca agtcctcaga acgccaaacg aatcattacc ctgcttgcca
     3661 ggaaggttga aggaagcagg tatattcagg gggtgatgat tgatacatcc aaactttgtg
     3721 gaattgctga taaatcaagt tttctgcatt tttactcatc aattgcttcg tcacatcgtt
     3781 tgctgatgat tgagcttagc agggagggcg ctattaaagg atgcaaccga attaccgaaa
     3841 aatcttttgg ctacagcctg gccgaactcc agaacccaca gctattaccg tctcttttca
     3901 caaacgccga ttcttttaaa cagctggtta cctatgcaaa ttcaggaggt tctaaacctt
     3961 ttgtgtgtag agttagaaac cgcgaaggac aggttaaaga tatttcgtgg attctgctta
     4021 aacttccgga agtggttggt atggaaggca ataccctaat tgggcaggat gtaaccaggc
     4081 aggttcaggt aaattttgaa gttaacctac ttcaacaaag gtaccaggct attaagttgg
     4141 ctaatgccga gttgcagggt gctgctaatt tcgagggtgt tttttcgtgc tttgggcaac
     4201 agcttgagcg gatattcccc agcatggtaa cagtggtatt gtcgtataat gctgccgatg
     4261 gctttattac ggttgaaggc atttttggca ttcagcacaa gacctgggaa tcgttaattt
     4321 tcgacttggg atggaaccct gttggccgcc ggttcctact gcacgataag gataaacatg
     4381 ccattaagtc ggctgcagcc tacagggtta acgaatcgtt taccggtatt atggaaggat
     4441 atatttccat gtcggcatca aaaattattg aacgatacat caatattgag gcgatatatg
     4501 cagagggttt ggtttctggc aaccacatgt acgggggagt cctcctactt aaaccagaac
     4561 ctgccgatga acccgattta accttactcg aagagctggc ttctgttgct gttaatacca
     4621 ttcaacggat tgatacagcc gagggtttac agcaacaaat ttttacactt aaatcaaaaa
     4681 ttgagcagaa agacgagcta ttcagccagc taagccatca gctaagaacc cccctgaatg
     4741 cagttttggg tttcagccag ctgctgctgg agttgaatca acccgatgat ttgcttaaaa
     4801 aatacatcgg gataatcaac agcaagggga agcaacttct ccggctggtt aacgacacca
     4861 ttgattataa ccttattgaa aagggcaacc tgactctggt aaaatcaact tttaatctta
     4921 accacctgct tcaacagctc caccgctctt tttcacagca gttcgaactt taccagaatg
     4981 aggggataaa gctaaaattt atacaaccag gaaatgccga aaacatcttt ctt
//
LOCUS       DAOOQX010000053         5000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_20176_length_5000_cov_3.595349, whole genome shotgun sequence.
ACCESSION   DAOOQX010000053 DAOOQX010000000
VERSION     DAOOQX010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20176_length_5000_cov_3.595349"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..2471)
                     /locus_tag="PLX53_01515"
     CDS             complement(255..2471)
                     /locus_tag="PLX53_01515"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOY71789.1"
                     /translation="MIISNVFWVVPIASVLALLFAYYFFRQMMKEDEGTDIMKKIALH
                     VRKGAMSYLKQQYKVVGLVFVVLVLLFALLAYGLHVQNGWTPFAFLTGGFFSGLAGFL
                     GMKTATYASARTANAAQKSLNRGLKIAFRSGAVMGLVVVGLAVLDISLWYLILNYFID
                     EPDPSQKLIVITTTMLTFGMGASTQALFARVGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPATIADNVGDNVGDVAGMGADLYESYAGSILATAALGASAFALFEGNMQYKAILA
                     PMLIASVGIVLSLLGIFIVRTKENATMRDLLRSLGRGVNVSSLLIALSTFGVLYGLGL
                     HHWLGISFSVVSGIAAGVIIGQSTEYFTSHSYKPTQRVSESSKTGPATVVISGIGLGM
                     VSTAIPVITITLAILMAFLCAINFDVKHMLEAQNIQLGLYGIGIAAVGMLSTLGITLA
                     TDAYGPIADNAGGNAEMSHLGPEVRKRTDALDALGNTTAATGKGFAIGSAALTALALL
                     ASYIEEIKIGLVRIGENVLTFANGETVETAKATIVDFMSYYQINLMNPIVLAGIFIGA
                     MMAFLFSGLTMNAVGRAAQKMVEEVRRQFREIKGIMTGETEPDYERCVAISTRGAQIE
                     MILPSSLAIIVPILTGLLLGVAGVMGLLTGGLGAGFVLAIFMANSGGSWDNAKKYIEG
                     GNFGGKGSDCHKAAVVGDTVGDPFKDTSGPSLNILIKLMSMVSIVMAGVTVAFSIF"
     gene            complement(2696..4234)
                     /locus_tag="PLX53_01520"
     CDS             complement(2696..4234)
                     /locus_tag="PLX53_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOY71790.1"
                     /translation="MLVKTFGSAVHGIKATTITIEVSVSQGINFFLVGLPDNAVKESQ
                     QRITSALETNGYRFPKKRVVINMAPADIRKEGSAYDLPLALGILAASEQVNPQMLPDY
                     VVMGELSLDGSVQPVRGVLPIAIQAREEGFKGFIVPIQNAREAAVVNDLDVYGVSSIR
                     EVIDFFNGNESLKPTIINTREEFFANANSYDVDFADVKGQENVKRALEVAAAGGHNII
                     MIGPPGAGKTMLAKRIPTIIPPLTLREALETTKIHSVAGKIDRDTSLMTRRPFRSPHH
                     TISDVALVGGGQYPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPLEDRVITISRAK
                     FSVEYPASFMLVASMNPCPCGNYNNPFKECVCSPGMVQKYLNRISGPLLDRIDIHIEV
                     IPVPFEKLATTINSETSETIRKRVIKARQVQEERFVEWKEVYCNAQMTSKLIRKFCEL
                     DSAGSSLLKTAMERLNLSARAYDRILKVSRTIADLDDSPSIKPEHLAEAIQYRSLDRE
                     NWAG"
     gene            4645..>5000
                     /locus_tag="PLX53_01525"
     CDS             4645..>5000
                     /locus_tag="PLX53_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOY71791.1"
                     /translation="MKISQFNISNFKVDGGAMFGVVPKTMWGKVYPSDENNLIPLSLK
                     SLVVEANGRVVLIDNGIGSKQDAKFMSHVYAFGGEGLLPGLAARGYRPEDVTDVILTH
                     LHFDHCGGGIFRDSHG"
BASE COUNT         1299 a         1168 c         1205 g         1328 t
ORIGIN      
        1 tcggcaacgc cctgaacgga ttttatgcaa cgttcaatgt aatcctcctc gttgtaagct
       61 atgattacgg ctgaaatttg ggtcatgtta ttttgaaata attctgacaa atggcgtgta
      121 aaagtaagat tttttcaata acacggttgc tggtttaacg aatttaattg ccatcaggtt
      181 agaggggatg cagggggtag agagaaaaaa gcccagctgg atatgctggg cttttaaagg
      241 attcatggct attcctaaaa aatgctgaag gccactgtaa cccccgccat aactatggac
      301 accatgctca tcaacttgat gagaatgttg aggcttgggc ctgaggtatc cttgaatggg
      361 tcgcctacgg tatcaccaac cacggcagcc ttatggcaat cggaaccctt acccccgaag
      421 ttccccccct cgatgtattt ttttgcatta tcccaagaac ctcccgagtt ggccatgaaa
      481 atggccagca caaatcctgc cccaagtccg ccggtaagta agcccatcac gccagctaca
      541 cccagcagca aaccggtaag tattggtaca ataatagcca atgaagaggg aaggatcatt
      601 tcgatttgcg ctccccgggt agagatggcc acacaccgct cgtaatcggg ctcggtttcc
      661 cctgtcatta tccctttaat ttccctgaac tgcctgcgaa cctcctccac catcttctgg
      721 gcagcgcgac caaccgcatt catggtcaac cccgagaaga ggaatgccat catggctcct
      781 atgaaaattc cggcgagtac aatgggattc atcaggttta tctggtagta gctcataaaa
      841 tcaacaatgg ttgccttggc agtctcaacg gtttcaccat tggcaaaggt gagaacgttt
      901 tcacctattc gcacaaggcc aatcttaatt tcttcgatgt acgaagctag cagggccagc
      961 gctgttaggg cggccgaacc aatggcaaaa cctttaccag tggcagcggt agtgtttccc
     1021 aatgcatcaa gggcatcggt acgtttgcgg acttcagggc caaggtgact catctcggca
     1081 tttccccctg cattatcggc gattggcccg taggcatcgg ttgccagggt tattcctaaa
     1141 gttgacagca tccccacggc ggcaatgcca attccataga gccccagctg gatgttttga
     1201 gcttccagca tatgcttaac gtcaaagttg atggcgcata aaaatgccat caaaatggca
     1261 agggttatgg tgataaccgg tatggctgtt gataccattc ccaggccgat gcccgaaatg
     1321 acaacggtag ccggtccggt ttttgaactt tcggataccc tttgggtggg cttgtacgaa
     1381 tgggaggtga agtactcggt ggattgccca atgattacac ctgctgcaat tcccgataca
     1441 accgaaaatg aaattcctag ccagtggtga agcccaagtc cataaagaac tccaaaagtt
     1501 gagagggcaa ttagcagcga gcttacgtta acacccctgc caagcgaacg gagcaaatcc
     1561 ctcatggtgg cgttctcctt ggtgcggaca atgaaaatgc ccagcagcga tagcacaata
     1621 ccaaccgacg caataagcat gggagctaaa atagctttgt actgcatgtt accttcaaat
     1681 agtgcaaatg ctgatgcccc tagtgcagca gtagcaagga ttgaacctgc ataggattcg
     1741 tacaggtcgg cacccattcc ggctacatcg cccacgttgt cgcctacatt atcggcaatg
     1801 gtggcagggt tacggggatc atcctcggga atgccggctt caaccttacc aacaaggtcg
     1861 gcgccaacat cagcagcctt ggtgtaaatt ccacctccaa cacgggcaaa tagcgcctgg
     1921 gtggatgctc ccataccaaa ggtaagcata gtagtagtga tgacaattag cttctgggat
     1981 gggtcgggct cgtctataaa gtagttgagg ataagatacc atagcgaaat gtcgagaacc
     2041 gccagtccaa ccaccaccag ccccataacg gcacccgagc gaaaagcaat tttcagacct
     2101 cggttaagcg atttttgtgc agcatttgct gtgcgggccg atgcgtaagt ggcggttttc
     2161 atacccaaaa atccggccaa gcccgaaaag aagccgccgg taagaaatgc gaatggcgtc
     2221 catccatttt gaacgtgaag accataggcc agaagtgcga agaggagaac aagcaccaca
     2281 aaaacaagcc caacaacttt atactgctgt tttaggtacg acatggcgcc ctttcgcaca
     2341 tgaagggcaa tttttttcat gatatccgta ccctcatcct ccttcatcat ctgcctgaaa
     2401 aagtagtagg caaatagcag ggcaagcacc gatgcaatcg gaacaaccca gaatacattg
     2461 gaaattatca tactagttgt ttttaggttg aattatagtt aggttataaa tcagaaattc
     2521 aacaatatgc aagtatggtt tagcaccaaa tcccttttat ttttaaactc gccaatatac
     2581 aaagtgttga atggtaatga aagcatattt ggtttttttt cgggttgact cgaagtgttt
     2641 ttattcaact tttagtaatt gggagagttt aaaaaatagc ataagataaa agcctttacc
     2701 ctgcccagtt ttctctgtcg aggcttcggt actgtatggc ctcggccaga tgctcgggtt
     2761 tgatactggg tgaatcatcc aaatcggcaa tcgtacggga aacttttagt attctatcgt
     2821 aggcccttgc tgagaggttg agccgttcca tggcagtttt tagcagcgac gatccagcac
     2881 tgtcgagctc acagaatttt ctgatcagct tggaggtcat ctgggcattg cagtaaactt
     2941 ccttccactc tacaaatcgt tcttcctgaa cttggcgtgc tttaattacc cttttacgga
     3001 tggtttcgct ggtttcggag tttattgttg tggccagctt ctcgaacggt acgggaataa
     3061 cctctatgtg tatgtctatt ctatcgagga gggggccgga aattcggttt aggtactttt
     3121 gcaccatgcc tggtgagcaa acgcattctt taaaagggtt gttatagttt ccgcatggac
     3181 aggggttcat ggaggcaacc agcatgaagc tggcggggta ctccacggaa aattttgctc
     3241 tggatatggt gataacccta tcctcgagcg gttgacgcat gacctccagc acggttcgtt
     3301 taaattctgg cagctcgtcg aggaatagca cgccattatg agccagcgaa atttcgccgg
     3361 gttgggggta ctgtccacca ccaaccagcg ctacatctga gatggtgtgg tgagggctac
     3421 ggaaaggacg tcgggtcatt agagaggtgt cgcggtctat ctttccggca acagaatgaa
     3481 ttttcgtggt ttcgagcgct tcgcgcaacg taagaggggg aatgatggtt ggaatccgtt
     3541 tggccagcat ggttttaccg gcgccaggag gcccaatcat gatgatgttg tgcccaccgg
     3601 cagcggcaac ctccagcgca cgcttcacat tttcctgccc ctttacatcg gcaaagtcaa
     3661 catcgtagct gttggcgttg gcaaaaaatt cctcacgggt attgataatt gtgggcttta
     3721 ggctttcgtt tccgttgaaa aaatcaatga cttcccgaat tgatgatacc ccgtaaacat
     3781 ccaaatcgtt tactacggct gcttcccgtg cattttgtat gggcacgatg aaccctttga
     3841 agccctcctc acgagcctga atggcaatgg gaagcacccc ccttacgggc tgaacggagc
     3901 catcgagcga aagttcgccc ataacaacgt aatccggcag catttgaggg tttacctgtt
     3961 ccgatgctgc cagaatcccc aatgccagtg gtaggtcgta ggccgagccc tccttcctta
     4021 tatcggccgg agccatgttg ataacaaccc gtttctttgg gaacctatat ccatttgttt
     4081 ctaaagccga ggtgattcgt tgctggcttt ccttcaccgc attgtccgga agccctacaa
     4141 gaaagaagtt tatcccttgc gaaacactca cctcaatggt tatggtggtg gcctttatgc
     4201 catgaacagc acttccaaaa gtctttacaa gcatatatgc caaaatttat atcacttgca
     4261 atttactcga aattaatgaa tagcggaaat acgaagtacc tttatgctca taagtgtagc
     4321 caaaaattta ttggcgcctg ttgattttgc cttaagcaga ttttttatca aacagttttt
     4381 gtacagaaat gcacttaccc tttgaatttt tcccagattc cctaattcaa tggttgagtt
     4441 ttaaccaatc aacttgacaa taaatgctgc aagtagagta tgctttgccc atttacttgt
     4501 tggggagctc attttttatt tcagagtgaa cgggaatgaa ctatatttcg ccagctatgg
     4561 cattaagcgc tatgttgtgc ccgaggagtt tgtgttgcgt gttacaggaa ttgtaacttt
     4621 gtaaaatatt ttaactcaac atgtatgaag atttcgcagt ttaacatatc aaactttaag
     4681 gttgacggtg gggcaatgtt tggcgtagtt ccaaaaacta tgtggggtaa ggtttacccc
     4741 tctgatgaga ataacctgat acccctttcg cttaaatcgc tggttgttga ggctaacggg
     4801 agggttgtgc tgattgacaa tgggataggc agtaagcagg atgcaaagtt tatgagccat
     4861 gtttacgctt ttggcggcga gggcttgttg cccggtttag cagcaagggg ctacaggcca
     4921 gaagatgtta ccgatgtgat tctcacccac ctgcattttg accactgcgg tggagggatt
     4981 ttccgtgatt cccatgggaa
//
LOCUS       DAOOQX010000054         4938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_20467_length_4938_cov_3.609461, whole genome shotgun sequence.
ACCESSION   DAOOQX010000054 DAOOQX010000000
VERSION     DAOOQX010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20467_length_4938_cov_3.609461"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1924
                     /locus_tag="PLX53_01530"
     CDS             <1..1924
                     /locus_tag="PLX53_01530"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HOY71792.1"
                     /translation="LQKSMYKQAIEHLNEALALQEQVVDPLAEASTLTEIGNLNLQQG
                     NIAEALRHYLMALKLRQTYGQDEDIAKSLTSIGLTYRRLGMYKSALKYLEQAREIIQD
                     MKGSMNDAAYILQNLGHIYLDLNSPDKAIATYKEALTLKEKSGDSIGAAKILKNIAQA
                     QLKSSQPDQARTSISQALKLVTRTHSTSDIADLYNELGNVERQAKNPGMAIKHYHTAI
                     ENYKALNNNEGVALCLRKIGEIQVGQKQLDEARKNINSSIEIGKQSDNKYLLSYGYLA
                     QSELYRAQGLFDKALESYIKHIGIRDSLESVKRNEANLEAQIDLELDQTKTEIKVMEA
                     EVEALRQKTALDKAIIEKQRLFRNSLIAIILLVVTAASIAFYSLIQKRRYAKLLEEKI
                     EEIRLVNERLNQSESELRQTIKTKDKLFSIIAHDLRSPFTALVGLSEVMATNLESLSP
                     DEIREFSHHIHRSATEVLNLTENLLSWARSQTGKIELSPKPINLKDLINQVFSVAAIP
                     AQEKKIKLRESIPANLTVFADFDTLQTVIRNLVSNAIKFTPQGGSIGISAKNSSNSMV
                     EIAITDTGVGIDPENQAKLFRVDGFTTKGTNQENGTGLGLILCKEFVELNHGTIEVSS
                     TPGLGTTFTISLPSNN"
     gene            1929..2135
                     /gene="xseB"
                     /locus_tag="PLX53_01535"
     CDS             1929..2135
                     /gene="xseB"
                     /locus_tag="PLX53_01535"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOY71793.1"
                     /translation="MTKKPQRYIDAINEIEQIITEIENNELDVDDLTEKIRRASELLK
                     FCKTKLRATEEEIQKIISDFEEDS"
     gene            2344..3951
                     /locus_tag="PLX53_01540"
     CDS             2344..3951
                     /locus_tag="PLX53_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOY71794.1"
                     /translation="MAYSIVAICGSERLSREIKSIVGIAEFDFDLHFVTDDTNIITNL
                     TQLNPDIVLFDVSYSEYAAPDLLTIIKNDIKTESVPILAIADFLQPERIQALFIAGAD
                     DFISQPLAAAELIQRTMIGIQRGRMLKKLKEQSRQFNSITDAISKAGNSMIVINHLGK
                     ITWVNEGFKRLYECPLDYFVSIFGDNLFDSSTNPVTREALDKCRKNGEYVVYESKWVT
                     PQGKEKFIQTSLTPIFDSLQNLSQIIAIETDISDLKAIEKDLEEKHEHLLTITENLKS
                     VNAVLAEHQERIQKQALQLEEEKQKSENLLKNILPWEVAYALQKKGSYKPKKFKEVSV
                     MFTDFVGFSKISSNYGDIDEFLSVLGGFFEVFDEITSQRFIEKIKTIGDSYMCVGGLP
                     RVNRSHPFDTVLAAIEIQRFIHDKAVQAAMEGKPVWNLRIGIHTGSVIAGVIGKRKFA
                     YDIWGDTVNIASRMETASEPGKVNISQTTYSYIKDYFVCTSRGKVPAKNLGEIEMYFV
                     ERIKPEYSEDELGLIPNSSFRKIVASF"
     gene            complement(3967..4461)
                     /gene="cdd"
                     /locus_tag="PLX53_01545"
     CDS             complement(3967..4461)
                     /gene="cdd"
                     /locus_tag="PLX53_01545"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOY71795.1"
                     /translation="MSESNSFQFRYNRLRGKEELAPNERELVDRAVAALSSSYSPYSH
                     FRVGAAVLLENGEIITGSNQENGAYPSGLCAERVALFYAGARYPGIPVVSMAVVASSG
                     DGLTENPVTPCGACRQVMSEYRVVSGKPFAILMVGSNEIVRVEDVEQLLPFSFSLSNG
                     MKRK"
     gene            complement(4627..>4938)
                     /gene="aspA"
                     /locus_tag="PLX53_01550"
     CDS             complement(4627..>4938)
                     /gene="aspA"
                     /locus_tag="PLX53_01550"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245276.1"
                     /note="catalyzes the formation of fumarate from aspartate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOY71796.1"
                     /translation="IVHSIFESVEMLKNGMNTLKYRCIDGITANEERCRSMVHNSIGL
                     VTALNPYIGYEASTQLAKEALSSNRSVYQLVLEKGLLTREQLDRILAPENMIKPGKMK
                     L"
BASE COUNT         1561 a         1186 c         1031 g         1160 t
ORIGIN      
        1 cttgcaaaaa tcaatgtaca aacaggccat cgaacacctc aatgaagctt tggcccttca
       61 ggaacaagtt gttgacccgc tggcagaggc atcaacccta acagaaatag gtaaccttaa
      121 cctgcagcag ggcaatatag ccgaagcgct gcgtcactac ctaatggcgt taaaacttcg
      181 gcaaacctac gggcaggatg aggatattgc caaatcgctc accagcattg gccttaccta
      241 ccgaaggttg ggaatgtata aaagcgccct taaatacctt gaacaggcaa gggaaataat
      301 tcaggacatg aagggtagca tgaatgatgc tgcatacatc ctacaaaacc tggggcatat
      361 ctaccttgac ctcaactccc ccgacaaggc aattgccaca tataaggaag ccctgactct
      421 taaagaaaaa agcggcgata gcataggcgc tgctaaaatt ttaaagaaca ttgcccaggc
      481 ccagcttaaa agcagccaac ccgaccaagc tcgtacatca atttcacaag ccctcaagtt
      541 ggtaacccga acacatagca ccagcgatat tgcagatcta tacaacgagc tgggtaatgt
      601 tgaacgacag gctaaaaatc ctggcatggc aatcaaacat taccacacag ccattgaaaa
      661 ctataaagct cttaataaca acgagggagt tgccctttgc ctaagaaaaa tcggagaaat
      721 tcaggttggg caaaaacagc tggatgaggc aagaaaaaac atcaacagct caattgaaat
      781 tgggaaacaa tcagacaaca aataccttct atcatacggc tatctagctc aaagtgagct
      841 ataccgcgca caaggccttt tcgataaagc cctggaaagc tacataaagc atataggcat
      901 tcgcgacagc ctagagagcg ttaaaagaaa cgaggcgaac ctagaggctc agatagacct
      961 ggagctcgac caaacaaaaa ccgagattaa ggttatggaa gcagaggtgg aagcgctcag
     1021 acagaagact gctctggata aagccataat cgagaagcaa agattattcc gcaactcgct
     1081 aattgccata atcctacttg tcgtaactgc ggccagcata gctttctaca gccttattca
     1141 gaaacggcgt tatgccaagc tacttgagga gaaaattgag gaaatcaggc tggtaaacga
     1201 aaggctcaac cagtctgaga gcgagctgcg tcaaaccatt aaaactaagg ataagctatt
     1261 ttcaatcatt gcccatgact tgcggagccc atttaccgcc ttagttggac tatcggaggt
     1321 aatggcaaca aacctggaga gcctttcgcc cgatgagatt cgtgagttca gccaccacat
     1381 ccaccgctcg gcaaccgagg tgctcaacct gactgagaac cttctaagct gggcaagaag
     1441 ccaaaccgga aaaattgagc tctcgccaaa accaattaat ctcaaggacc ttatcaacca
     1501 ggttttttcg gtagctgcga ttcctgcaca ggaaaagaaa atcaaacttc gggaaagcat
     1561 tcccgccaac ctaacggttt ttgccgattt cgataccctc caaaccgtta tcaggaattt
     1621 ggtgagcaac gccattaaat tcaccccgca gggtggatca atcggaatat cagcaaaaaa
     1681 ttcaagtaac agcatggttg aaatcgctat taccgataca ggcgttggca ttgatcctga
     1741 gaatcaggcc aaacttttca gggttgacgg gttcaccacc aagggtacta atcaggaaaa
     1801 cggaacagga ttggggctaa tactctgtaa ggaattcgtt gagttgaacc atggaactat
     1861 tgaggtaagc agcacaccgg gacttggaac aacattcacc atttcattac cctcaaataa
     1921 ttagaactat gaccaaaaag ccacaaaggt atattgatgc cattaacgaa attgagcaaa
     1981 tcatcacaga aattgaaaac aacgagctgg acgtggacga cctgaccgaa aagataagaa
     2041 gggcctctga gctgctcaaa ttctgcaaaa ccaagctccg ggctaccgag gaggaaatac
     2101 aaaaaatcat aagcgatttt gaagaggata gctaaaagag ctgcccaagc ctcattcgcc
     2161 ctttatgact gttggacaat gtgtacccct tcatcaacat ctacaaatag ttctatcaaa
     2221 aaattgttaa tgatattttt acccttctct ctgtcctaaa ctactcctat aaacttccag
     2281 gcaaaaccct gaagaatatt tcaaaaattt aactttattt gcatataggt tgaatagagc
     2341 attatggctt acagcatagt tgcaatttgt gggtctgaaa ggctctcccg tgaaattaag
     2401 agcatcgtgg gaattgccga atttgatttt gatttgcact tcgttaccga cgacacgaat
     2461 atcatcacca acctaacgca gctcaacccc gatattgttc tttttgatgt ttcctattcg
     2521 gagtatgcag cgccagatct gctcaccatt attaaaaacg atataaaaac cgaatcggta
     2581 cccatactgg ccatagccga tttcttacaa cctgagagaa tccaagccct tttcattgcc
     2641 ggtgcagacg actttattag ccagcctctt gctgctgctg agcttattca acggaccatg
     2701 ataggcattc aacggggaag aatgctgaaa aaactaaaag agcaatcccg gcagttcaac
     2761 agcattactg atgccattag caaggctggt aactccatga ttgttatcaa ccacctgggt
     2821 aaaattacct gggttaacga agggtttaaa cggctatacg aatgcccgct cgactacttt
     2881 gtatccattt tcggtgacaa cctatttgat tcctcaacaa acccggttac acgagaggca
     2941 cttgacaaat gccggaaaaa tggcgagtat gttgtttatg agagcaaatg ggttacccct
     3001 cagggcaagg agaaattcat tcaaacatcg cttacgccga tttttgatag cctccagaac
     3061 ttaagccaaa taatcgccat cgaaaccgac atctccgacc tgaaagccat tgaaaaagac
     3121 ctggaggaaa agcatgaaca cctgcttacc atcaccgaaa acctgaagag cgttaacgcc
     3181 gtgctggcag agcatcagga aagaattcag aaacaagccc tacagctcga ggaggagaaa
     3241 caaaaatctg aaaatttact gaaaaacatc cttccatggg aggttgcata tgcccttcag
     3301 aaaaagggat catacaaacc taagaagttc aaggaggttt cggtaatgtt caccgatttt
     3361 gttgggttct caaaaatcag cagcaactat ggtgatatag acgagttcct cagtgttttg
     3421 ggtggtttct ttgaggtatt cgacgagata acctcccagc gtttcatcga gaaaattaaa
     3481 acaattggcg atagctatat gtgtgtagga ggactcccca gagtaaaccg tagccaccca
     3541 ttcgacactg tacttgctgc cattgagatt cagcgattta ttcatgataa ggctgtacag
     3601 gctgcaatgg aaggtaaacc ggtatggaac ctccgtattg gcattcacac cggaagtgta
     3661 attgcaggag taatcggaaa acgcaaattc gcctatgata tttggggcga cacggtgaac
     3721 atagccagcc gaatggagac tgccagcgaa cccggaaaag ttaatatttc gcaaaccaca
     3781 tattcctaca ttaaggatta cttcgtttgc acttcgcggg gaaaggttcc cgcaaaaaac
     3841 ctgggtgaaa ttgaaatgta ctttgtggag cgcataaaac ccgagtactc cgaggatgaa
     3901 cttgggctaa tacccaactc ctccttcagg aaaatagtgg cctccttcta accttcagca
     3961 aaggccttac ttccgcttca tcccattgga gagtgaaaaa ctaaagggaa gcagctgctc
     4021 tacatcctca accctgacaa tttcgttact cccaaccatt agaatggcaa acggtttccc
     4081 tgaaactacc ctgtactcgc tcataacctg ccggcatgct ccgcatgggg ttactggatt
     4141 ctccgtaagt ccatctcccg aacttgccac aacagccatt gaaactacag gaattcccgg
     4201 atatcgagca ccggcataga acaacgcaac cctctctgca catagccccg aagggtaagc
     4261 accattctcc tggttgctgc ccgtgataat ctccccattc tcaagtagca cggctgcccc
     4321 tacccgaaag tgtgagtagg gtgagtagct cgacgacagg gcagcaacag ccctgtcaac
     4381 cagttctctt tcgttgggag ctaactcttc tttgcccctc aaccggttat accgaaactg
     4441 aaagctatta ctttcgctca tggaatgcaa attattagaa aacctacatt taccccacaa
     4501 atgtagaata aaaaactatt gactatttac tttaaaagga aaactaaaca tgcctggaga
     4561 aaattgtaca caatcgcctt agcattaagc agtcaagtat caaaatcccc catcagctcc
     4621 aggaatttag agtttcattt tgcccggttt tatcatattc tcaggtgcta aaatccgatc
     4681 gagctgctcc cgtgtaagca accctttttc cagcaccagc tggtaaacac ttcggttaga
     4741 tgaaagcgct tcttttgcaa gctgggtaga ggcctcatag ccaatatagg ggttaagagc
     4801 agtaaccaac ccaatactgt tgtggaccat gctcctgcac cgttcctcgt tggcagtaat
     4861 cccatctata cagcgatatt ttagcgtatt cataccattt ttcagcattt cgaccgattc
     4921 aaaaatgctg tgcacgat
//
LOCUS       DAOOQX010000055         4923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_20542_length_4923_cov_5.022802, whole genome shotgun sequence.
ACCESSION   DAOOQX010000055 DAOOQX010000000
VERSION     DAOOQX010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20542_length_4923_cov_5.022802"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="PLX53_01555"
     CDS             complement(<1..1118)
                     /locus_tag="PLX53_01555"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOY71797.1"
                     /translation="MKKYYTLLVILLLFLNATQSQPLTGTGSVANKQLLHHVSILASD
                     SMGGRFPGTTYDRISAKYIRDQLQSYGLSLQQKNGYQFFAIQTFNPNRATSTFLVVNG
                     KRLQYGNDYTALATSGNDSLCATAVFVGYGRTGGSADPDDYKNVSVKKKWAVMLVPSL
                     KVQDASSGELQQVDLSCINGAVGHEAAGIILIASEAENRFISMGSQQQALNLPVFILN
                     GKSSELILKSIGPADEVIGILERGGQNLSQDLPLTICGKAGKPYNEIETQNVVGVLRG
                     SDPNLADEYIVVGAHYDHLGMGGKEANSRMPDTVAVHNGADDNASGVAVMLEIAREMA
                     SQKKQLKRSVVFAAFGAEEMGLVGSQRFLDSAVVPIPN"
     gene            1401..1760
                     /locus_tag="PLX53_01560"
     CDS             1401..1760
                     /locus_tag="PLX53_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71798.1"
                     /translation="MEAKRFSERKLILFTVVLMIAAGLVRLVSYKVGVVLFYLSFLPF
                     IIHRLNFYHNHRGKLTDVDRRRRVTLALLLLMMLLNMVGWQNVEFILLIILAVDYMLI
                     NNRRSNGEKADDFGVKH"
     gene            1964..2611
                     /locus_tag="PLX53_01565"
     CDS             1964..2611
                     /locus_tag="PLX53_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOY71799.1"
                     /translation="MNVNSFFQFLAPKENKFYPHFNAASDNLVEVSLLLKKMMETDTL
                     DERLSFISKIKELERKGDDITHKMFDLLNGSFITPFDREDINALISSIDDVVDYINGS
                     AQQFQLYKPVSIPAEYIKMAEHIIEGSKTIQKAIYHIKELKNPVEIMDACIHVNEIEN
                     QADETYHRGIFQLFEHETNAIELIKKKDILQLLEKATDKLEDVSDVIKSILIKQS"
     gene            2617..3606
                     /locus_tag="PLX53_01570"
     CDS             2617..3606
                     /locus_tag="PLX53_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOY71800.1"
                     /translation="MSTLVIVIVGLAFLYDFINGMNDAANSVATVVATRVLSPFVAVL
                     WAALFNFAAYFLFGLNVANTMGKGIIQPHVVEPWLVFSALLGASAWSYFCTHFGLPIS
                     VSHALIGGMVGPAWFAYGASSVVASGILNVVIFIFVSPLIGFVLGYLLMILIKHILKR
                     VKPANVDSWFRALQLLSSALFSLGHGSNDAQKTMGIIAVLLFSTGYLGSEFYVPEWVA
                     FSCYIAIALGTLTGGWKVIKTMGVNLTDLKPVHGFAAETAGAITLGMSAFMGIPVSTT
                     HTISGSIMGVGVTRRVSAVRWKVAQNIIGAWILTIPMTMIISGLLYLLIRVFV"
     gene            complement(3698..4552)
                     /locus_tag="PLX53_01575"
     CDS             complement(3698..4552)
                     /locus_tag="PLX53_01575"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOY71801.1"
                     /translation="MVAVRTGSRLRVGVGRVCACFGVTRDAYYKLAKRLTRRRAVADR
                     VVELVQEERRLQPRVGTRKLHVAMREQLGAEGIRMGRDKLFTLLRERGLLVAPRRASC
                     RTTNSYHRFHKYGNCIRGMQVTRPNQVWVSDITYIRTARGFCYLALVTDLYSRRVLGY
                     DVSDSLELAGCLRALKLALQVARPAPGLIHHSDRGVQYCSAAYIECLYKNGISPSMTQ
                     EHHCYENAVAERVNGILKDEFFLDQCFLDLKQARKAAADAVRIYNFKRLHVSLGYKTP
                     DVVFKSVA"
     gene            complement(4552..>4923)
                     /locus_tag="PLX53_01580"
     CDS             complement(4552..>4923)
                     /locus_tag="PLX53_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOY71802.1"
                     /translation="MTKNDYVVKRYSEGFRLKILSELSSGKYSKNELAKLYGLSVATI
                     SRWVAGHKRIDLMNKRVTVQNPDEASRLQAMQKELEQLKKLLLRKDLELLVSESYLEA
                     VARQHGYKDVEELKKNLRIQP"
BASE COUNT         1180 a         1188 c         1162 g         1393 t
ORIGIN      
        1 atgttcggga tgggaacaac cgccgagtca aggaagcgct gcgacccgac aagccccatc
       61 tcctcggctc caaaggcagc aaaaaccacg ctgcgtttaa gctgtttttt ttgtgaggcc
      121 atttctcgtg ctatttccag catgacagcc actcccgagg cattatcatc ggctccgttg
      181 tgcaccgcaa ccgtatcggg catccggctg ttggcctcct tgccccccat gcccaagtgg
      241 tcgtagtggg cacctaccac aatgtactcg tcagccaggt tggggtcgct tcctctcagg
      301 acacctacaa cgttttgggt ttctatctca ttgtagggtt tgcctgcttt tccgcaaatg
      361 gtaaggggca aatcctgcga caaattttgt ccaccccttt ccagtattcc gatcacctcg
      421 tcagctgggc caatactctt aagtatcaat tctgatgatt tcccatttag aataaaaacc
      481 ggcaggttga gagcttgctg ctgacttccc atggaaatga agcgattttc agcttcgctg
      541 gctatcagga ttatgcccgc tgcttcgtgc cctacagccc cattgatgca gctgaggtca
      601 acctgttgaa gttcaccgct ggaggcatct tgcaccttca agctggggac aagcataact
      661 gcccactttt tcttcaccga aacattttta tagtcatctg ggtcggcaga cccgcccgtg
      721 cggccgtagc ctacaaaaac tgccgtagca caaagcgaat cgtttccgct tgtggcaagg
      781 gcagtgtagt cgttgccgta ttgaagtctt ttgccattaa ccaccaggaa agtgcttgtt
      841 gccctatttg ggttaaaggt ttggatggca aagaattggt agccgttttt ttgttgaagg
      901 gagagaccgt agctctgaag ctggtcacgg atgtacttgg ccgaaatcct gtcgtaggtt
      961 gtgccaggaa accgtcctcc catgctgtct gaagccaaaa tacttacatg atgtaaaagt
     1021 tgcttatttg ctacactacc ggttcctgtt agaggttgcg attgggtagc gtttaaaaat
     1081 aaaagtaaga ttaccagcag cgtgtaatat tttttcatgt ttattgtttg gtagttaccc
     1141 cgttataaat tcaaccgtca aatatagtga tatatgaaat aaaatcagta agtgttcacg
     1201 gcctgcaatt taagtgccac ggtaactagt cgggattgcc aaaacaccgt tagccattcc
     1261 ggctggttga ccaaaaaata tctatttttg cttgctaaac atgttgacag gtggtgcgtg
     1321 ggcgggttca aaagttgttg gtttttcatc agccgggcag ctgcattgaa tcccaaattc
     1381 tgattacttc taattaggtt atggaggcta aaagattttc ggagcgtaag ctaatccttt
     1441 ttaccgtggt gctgatgatt gcagccggac tggttaggct ggttagctac aaggtgggtg
     1501 ttgttctgtt ctacctgtcc ttcctgcctt ttattattca cagattaaac ttttaccata
     1561 accacagggg taagcttacc gatgttgacc ggcgccggcg ggttacgtta gcccttctgc
     1621 tgctgatgat gcttcttaac atggttggat ggcaaaacgt agaattcatc ctgcttatca
     1681 tcctggcagt ggactatatg ctgattaata accgtaggag taatggtgaa aaggccgatg
     1741 attttggggt aaagcattaa acaacttgag ggttcattat ttgcttccag cacttggtta
     1801 accaaaaaaa ttatgtaata tttgattatc aacatatttg cttaatacca agcagttaaa
     1861 gttaacttaa caattactta acattggaga gattctcata gcttattttt gtgtttcttt
     1921 ataattgaaa atcaaacata taaacttaac atatttcaga aagatgaatg tgaacagttt
     1981 tttccaattt cttgccccca aggaaaacaa attctatcct cattttaatg cagcgtcgga
     2041 taacctggtt gaagtttcgc tgcttttaaa gaaaatgatg gagaccgata cattagatga
     2101 acgcttgtcg tttatcagta aaattaagga gcttgaaagg aaaggggacg atattacgca
     2161 taaaatgttc gatctactta acggatcgtt tatcacacca tttgaccggg aggatatcaa
     2221 cgcccttatc tcttccattg acgatgtggt agactacata aacggttctg cgcagcaatt
     2281 ccaactgtac aaacctgtat cgattcctgc ggaatacatc aagatggccg agcatataat
     2341 tgaagggagc aaaaccatac aaaaggcaat ttaccatatt aaggagctga aaaacccggt
     2401 agagattatg gatgcctgca ttcatgttaa cgagatagaa aatcaggccg acgaaacata
     2461 ccaccgggga atattccagc tttttgaaca cgaaaccaat gccattgagc tgattaagaa
     2521 gaaagatata ttgcagctgc tcgaaaaagc taccgataag ctagaggatg tttccgacgt
     2581 tatcaagtcc attttaatca agcaatcata gccagcatga gcaccctagt aattgttatc
     2641 gtcggattag cctttttgta tgattttata aacggcatga atgatgccgc aaactcagtt
     2701 gctaccgttg ttgccaccag ggtgttatcg ccttttgtgg ctgtgctttg ggcagcattg
     2761 tttaactttg ccgcttactt tttgtttggg ctcaacgttg ccaacaccat gggcaaaggt
     2821 attattcagc cccatgttgt tgaaccatgg ctcgttttct cggctctcct tggtgcatct
     2881 gcatggtcgt acttctgtac ccattttggc ctaccaataa gcgtttcaca cgccttaatt
     2941 ggggggatgg taggtccggc ttggtttgcc tatggggcta gtagcgttgt agcttcggga
     3001 atactcaatg tggttatctt catttttgtt tcacccctta taggttttgt tcttggatat
     3061 cttttgatga tcctgattaa gcatatcctc aaaagggtta aacctgcaaa tgtggactcc
     3121 tggttcaggg cgcttcagct tttatcgtct gccctcttca gccttgggca tggtagcaac
     3181 gatgcacaaa aaacaatggg tattattgca gtcctccttt tcagcacggg gtatctcggc
     3241 tccgaatttt atgttcccga atgggtggct ttttcatgtt acatcgccat tgcgctgggc
     3301 acattaactg gcggttggaa ggtgattaaa acgatggggg ttaacctaac cgatcttaag
     3361 ccggttcatg gctttgctgc agaaaccgcc ggtgcaataa cccttggaat gtctgccttt
     3421 atgggcattc ccgtgagcac aacccacaca atttccggct caatcatggg agttggggtt
     3481 acacgcaggg tctcggctgt ccggtggaag gtggcccaaa acatcatagg tgcttggata
     3541 cttaccattc caatgacgat gatcatttcc ggattgctct acctgctcat tcgggttttc
     3601 gtgtaacagg ctcttgaaag tttttatgaa agctattttg gtttgctcat tttcaaatgt
     3661 ctagtcctaa aatatgtcta caggttaata aataaaatta agcaacactt ttaaaaacaa
     3721 catcaggtgt tttatacccc aaagatacat gtaatctctt aaagttgtaa atcctcaccg
     3781 catcagcagc agcttttctg gcctgcttga ggtcaaggaa gcactggtcc aggaagaact
     3841 catccttcaa gatgccgttt acccgttcag ccacagcgtt ctcgtagcag tgatgctcct
     3901 gggtcatgct agggctaatg ccatttttat agaggcactc tatgtaggcg gcagagcagt
     3961 actgaacgcc tcggtcggag tggtgtatga gccctggagc agggcgggca acctgcagag
     4021 ctagctttag agctcgcagg cagcccgcca gctccaggga gtcgctgaca tcgtacccca
     4081 gcacccggcg ggagtacagg tcggtgacca gcgccaggta gcagaacccc ctagctgttc
     4141 ggatgtaggt gatgtctgag acccacacct ggtttggtcg ggttacctgc atgccccgga
     4201 tgcagttgcc gtacttgtgg aaccggtggt aggagttggt ggtcctgcac gaggcccgcc
     4261 ggggggcgac cagcaggcca cgctccctga gcagggtgaa gagtttgtcc ctgcccatcc
     4321 ggataccctc tgcgccaagc tgctccctca tggccacgtg gagcttgcgc gttcccaccc
     4381 ggggctgcag cctgcgctcc tcctggacga gctccaccac ccgatcggcc acggcacgcc
     4441 gacgcgtaag ccgcttggcc agcttgtagt aggcatcccg cgttacccca aagcaggcac
     4501 acacgcggcc tacgcccacc cgtagcctgg accctgtgcg aacggctacc atcagggctg
     4561 tatccttaag ttttttttta actcctccac gtccttgtac ccgtgctgcc tggcaaccgc
     4621 ctccaggtac gactcgctta ccagcagctc caggtccttc ctgagcagca gcttcttcag
     4681 ctgttccagc tccttctgca tcgcctgcag cctgctggcc tcatcggggt tctgtactgt
     4741 cacgcgcttg ttcatcaggt caatcctttt gtggccagcc acccatcggc ttatggtggc
     4801 aacgcttaag ccgtatagct tggccagctc gttcttggaa tacttcccgc tgctaagttc
     4861 ggataaaatt tttaacctga aaccctcgct gtagcgttta accacataat cgttctttgt
     4921 cat
//
LOCUS       DAOOQX010000056         4908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_20623_length_4908_cov_3.016691, whole genome shotgun sequence.
ACCESSION   DAOOQX010000056 DAOOQX010000000
VERSION     DAOOQX010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20623_length_4908_cov_3.016691"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="PLX53_01585"
     CDS             complement(<1..147)
                     /locus_tag="PLX53_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOY71803.1"
                     /translation="MKGIVLAGGAGTRLYPITKGVSKQMLPIYDKPMIYYPISVLMLA
                     GIRDI"
     gene            complement(154..1206)
                     /locus_tag="PLX53_01590"
     CDS             complement(154..1206)
                     /locus_tag="PLX53_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HOY71804.1"
                     /translation="MKHLLTGTAGFIGFHLAKRLLERGDEVVGLDCINDYYDVNLKYA
                     RLEVTGISRHKIEYGKLIQSSRYPNYRFIQLKLEDRETILKLFATEKFDRVCNLAAQA
                     GVRYSLQNPFSYIDSNIVGFMNILEGCRHNGVNHLVYASSSSVYGLNEEMPFSVHHNV
                     DHPVSLYAASKKSNELMAHTYSYLYQIPTTGLRFFTVYGPWGRPDMALFLFTKAILEG
                     KPIDVFNNGNMQRDFTYVDDIVEGVVRVIDSPPAGNPDWTGQSPDPGTSSAPYRVYNI
                     GNSKPVKLLDFIEAIEDALGKRAVKNFLPMQPGDVPATWADVADLAADFGYQPSTPVE
                     EGIRRFVEWYKKYYKI"
     gene            complement(1470..1859)
                     /locus_tag="PLX53_01595"
     CDS             complement(1470..1859)
                     /locus_tag="PLX53_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOY71805.1"
                     /translation="MIIERFEDIDIWQDSRELCIIIRGLCSKDGFAKDFTLKNQILSS
                     CGSIMDNIAEGFERGGNKELIQFLFISKGSCGETRSQIYRAFDFGYISKQECDCVVEM
                     LLSISKKLSGFISYLKKSDYKGIKYKS"
     assembly_gap    2201..2300
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2301..3595)
                     /locus_tag="PLX53_01600"
     CDS             complement(<2301..3595)
                     /locus_tag="PLX53_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOY71806.1"
                     /translation="MVKNICCIGAGYVGGPTMAVIALKNPDIRVNVVDINAERIAAWN
                     TDQLPVYEPGLLEVVKQARGRNLFFGTDVESAIREADMVFISVNTPTKTYGVGKGRAA
                     DLRFVELCARQIARVATSDKIVVEKSTLPVRTAQAIKTILAETGNSVKFQVLSNPEFL
                     AEGTAIGDLLEPDRVLIGGDQTPEGMAAIEALTQVYAKWVPRERIIQTRLWSSELTKL
                     TANAFLAQRISSINSISALCEVTGADVDEVAFAIGADTRIGPKFLKASVGFGGSCFQK
                     DILNLVYLCEYFNLPEVARYWEQVIILNDYQKRRFAQNIIASLFNTVSGKKIAMLGWA
                     FKKDTNDTRETASMYVADILMEDRAEIHVYDPRVSHEQMFADVDHLNTRPDSENRKLL
                     HPTTNPYEACLNAHAVALMTEWDEFKTYDWQRIYDGMMKP"
     gene            complement(4277..>4908)
                     /locus_tag="PLX53_01605"
     CDS             complement(4277..>4908)
                     /locus_tag="PLX53_01605"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY71807.1"
                     /translation="RYRISSIEPNLITDEIARFVAGSKKFLPHFHIPLQSGSNRILGL
                     MRRRYNRELFAEKIAMIRRLMPDTFFGVDVIVGFPGETEADFMDGYSFIREVNPAFLH
                     VFPYSERPNTAAALLPAKVDAREIKVRADRLGALSSELHRQFYISRTGQVHHVLLEAS
                     QKSGLMFGFTENYIRVAVPYNRNLAGKVVDVRLAALNPNGFMEGELMQP"
BASE COUNT         1279 a         1140 c         1094 g         1295 t
ORIGIN      
        1 aatatcccta atccccgcca gcattaaaac cgagatgggg tagtatatca ttggcttatc
       61 atagatggga agcatttgct ttgaaacccc tttggtgatg ggatacaatc gggttccagc
      121 acctccggcc aatacaattc ctttcatagt cttctaaatt ttatagtatt ttttgtacca
      181 ctccacaaac ctcctgatgc cctcctctac gggtgtggag ggttggtagc cgaagtcggc
      241 cgcgaggtcg gccacatcag cccaggtagc cggtacatcg ccgggctgca tgggcaaaaa
      301 gtttttcacg gccctttttc ccagggcatc ctcaatggcc tcaatgaaat cgagcagctt
      361 tacgggtttg ctgttgccga tgttgtaaac cctatagggg gcgctggatg tacccggatc
      421 gggtgattgg ccggtccagt caggattgcc tgccggcggg ctgtctataa cccgcaccac
      481 accctccaca atatcatcaa cgtaggtgaa gtcgcgctgc atgttcccgt tgttgaacac
      541 gtcaatgggt ttcccctcaa gaatggcctt ggtgaatagg aaaagcgcca tatcggggcg
      601 tccccagggg ccataaacgg tgaaaaagcg taaacctgtg gttggtattt ggtataggta
      661 gctgtaggtg tgggccatca gctcgttgct cttcttgctg gcagcgtaga ggctcaccgg
      721 gtggtcaacg ttgtgatgaa cggagaaggg catttcctcg tttagcccgt acacgctgga
      781 gctgctggca taaaccaaat ggttaacccc gttgtgcctg caaccctcaa gtatgttcat
      841 gaaacctaca atattgctat caatatagga gaatggattc tggaggctgt agcgcactcc
      901 ggcctgtgcc gccaggttgc aaaccctatc gaatttttca gtagcaaata attttagtat
      961 ggtttcccta tcctccagct ttagctggat aaacctgtag tttgggtatc ttgaactctg
     1021 aattaattta ccgtactcaa ttttatgcct gcttataccg gtaacctcta gccgtgcata
     1081 cttcaggttt acatcatagt agtcattaat gcaatccaaa cctaccacct catcaccacg
     1141 ttctagtaaa cgcttggcaa ggtgaaagcc aataaaacct gcagtaccgg tgaggagatg
     1201 tttcatgcgt tcaagattca agattccaaa ttcaagattc aaaattccag atttcagatt
     1261 taagaacttg tctgcctact ggcggattca aaaacttgtc cgcctactgg cggaacttgt
     1321 ccggctcctg acggattcca gattcaaaat tccaaattca agaacatgtc ctcctgctgg
     1381 cggaacttgt ccggctcctg acggattcaa aatttaagat tcaaaaacct gtccgcatac
     1441 tggcggaact tgtccgccta ctggcggatt caagatttgt attttattcc tttataatca
     1501 gattttttta ggtatgatat aaagccggat agttttttgc taattgataa aagcatttcg
     1561 actacgcaat cacattcctg ctttgaaata taaccaaaat caaatgctct ataaatttga
     1621 gatcttgttt ccccacaaga gcctttagat ataaataaaa attgaattaa ttctttattt
     1681 ccaccacgtt caaatccctc agcaatatta tccattatgg aaccacatga tgaaagtatt
     1741 tgatttttta gagtaaaatc tttagcaaat ccatctttac tgcataatcc tctgataatt
     1801 atacacaact ctctagaatc ctgccaaata tctatatctt caaatcgctc aattatcact
     1861 gagaagtcgg aaatttaaat ctggaatctg aaatctgaaa tctggaatct ggaatctgaa
     1921 atctgaaatt ttgaatctga aatctgaaat ctggaatctg gaattttgaa tctggaatct
     1981 gaaatctgga atctgaaatc tggaatctga aatctggaat tttgaatctg gaatctggaa
     2041 tctgaaatct gaaactaata actcttccca atcgccttaa ccttaaatcc tatctctatg
     2101 agttttttat ggtcgaggat gttacggcca tcgaagataa aggcgggttt catcataccg
     2161 tcatagattc gttgccaatc gtaggtttta aactcgtccc nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn ggtttcatca taccgtcata gattcgttgc caatcgtagg
     2341 ttttaaactc gtcccattcg gtcatcaggg caacggcatg ggcgttcagg caagcctcgt
     2401 aggggttggt ggtgggatgc aggagcttcc tgttctcgct atcgggtctc gtattcaggt
     2461 ggtcaacgtc ggcaaacatc tgctcatggg acaccctggg gtcgtaaacg tgaatttcag
     2521 ccctatcctc cattagaata tcggcaacgt acatcgaggc ggtttcgcgg gtgtcgttgg
     2581 tatccttctt aaatgcccag cccagcatgg cgattttctt tcccgagacc gtattgaaaa
     2641 gggaggcaat aatgttctgt gcaaacctac gtttctggta gtcgttgagg atgataacct
     2701 gttcccagta cctggctacc tcgggaaggt taaagtactc gcataggtaa accaggttga
     2761 gaatgtcctt ttgaaagcat gaacccccga aaccaaccga agctttaagg aacttaggtc
     2821 cgatacgggt atcggctcca atggcaaagg ccacctcgtc aacatcggca ccggtaacct
     2881 cacaaagagc tgatatgctg ttgattgacg atatacgctg ggcaagaaag gcgtttgcag
     2941 ttagcttggt cagctccgac gaccataagc gcgtttgaat gatgcgctca cggggtaccc
     3001 atttggcgta aacctgcgta agtgcctcaa tagcagccat gccctccggg gtttgatcgc
     3061 cgccaataag caccctgtcg ggttcgagca aatcgccgat ggcagtgccc tcggccagga
     3121 attcggggtt ggagagcacc tgaaatttaa cgctattacc cgtttcggca agaatggtct
     3181 tgatggcctg tgccgttcga acgggcaggg ttgatttttc aacaactatc ttatcggaag
     3241 tagccacccg ggcaatttgc cttgcacaca gctcgacaaa acggaggtct gcagcacgtc
     3301 ccttgcccac tccataggtt ttggttgggg tgttaacgct tataaatacc atgtcggcct
     3361 cacgaatggc actttcaaca tcggtgccaa aaaaaagatt tcggcccctg gcctgcttca
     3421 ccacctccag cagccccggt tcgtaaaccg gcagctggtc ggtgttccat gctgcaattc
     3481 gctcagcatt aatatccact acgtttaccc tgatatcagg attttttagc gcaataaccg
     3541 ccatggtagg gccacccaca tatccagccc cgatgcaaca gatgtttttc accataatga
     3601 aatgaatgaa taatcaaata tgtttaaatc atggataaag gacattcaag gataaagggt
     3661 aaaggataag ataattcaag attcaaaatt caagattcaa gatttcagat tcaagattca
     3721 agaacttgta agcctactgg cgaaacctgt cctcctacag gcggaacttg tccggctcct
     3781 gacggattca agaacttgtc cgactaccgg cggattcaag atttcagaac ttataagccc
     3841 actggcggaa cctgtccgcc tcccggcgga acttgtccgg ctcagaagga cttacaatta
     3901 aacggggatg gaagccaaaa caacgaagca attttaaaaa atagctatag ccaactatac
     3961 agcttcgccc tgtcagccac atctttaaat gatgaggcct gcggggaatt aatccacgaa
     4021 aataacactt ttcggtaaat gttagtaaaa aaaatgggtt ggtagcgttt cgggtaaatt
     4081 cggattatgc cccaattagc tagcaccaag caatagtatg ccattccatc cgtaataaat
     4141 ttattaaatt ctgattttgc ccataccttt taggtatctg ggttgagcgc gctgaacact
     4201 catttttggc ttcgccgggc tcgcaaatat cgtccggttg ttcatacgcg tctgtgcaaa
     4261 cccctacatg gtttattcat ggttgcataa gctcaccctc catgaaccca ttaggattta
     4321 gagcagctaa ccgaacatca accacttttc cagccaaatt acggttatat ggaacggcta
     4381 cccgtatata gttttcggtg aagccaaaca ttagccccga tttttgggag gcctcaagca
     4441 aaacatggtg aacctggccg gttcgggaaa tgtagaattg ccggtgcagc tcgctggata
     4501 gcgcgccaag gcggtcagcc cgaaccttaa tttcccttgc gtcaacctta gctggcagca
     4561 gagcggcagc tgtgttgggc cgttcggagt agggaaaaac gtgcaggaat gcagggttaa
     4621 cctcccgtat gaagctgtag ccgtccatga agtcagcttc ggtttcgccg ggaaatccca
     4681 caatcacatc aaccccaaaa aaagtatcgg gcataagccg gcgaatcatg gcaatttttt
     4741 cggcgaagag ctcccggttg tagcggcggc gcatcagccc gagtattctg ttcgaccccg
     4801 attgaagagg aatatggaaa tggggcagaa acttttttga accggccaca aaacgggcta
     4861 tttcatcggt tataaggttt ggctcgatgg aggagatcct gtagcgtt
//
LOCUS       DAOOQX010000057         4904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_20649_length_4904_cov_3.923283, whole genome shotgun sequence.
ACCESSION   DAOOQX010000057 DAOOQX010000000
VERSION     DAOOQX010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20649_length_4904_cov_3.923283"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="PLX53_01610"
     CDS             complement(<1..1351)
                     /locus_tag="PLX53_01610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOY71808.1"
                     /translation="MNWKNPEVIRKFKWWFWGIISFLLLFFLVIFILINLEFFGPMPT
                     FEELENPKSNIASEIISEDNVVLGNFYVQNRTFVGYDEISPNVIHALIATEDNRFYSH
                     SGIDAKGLGRVMVKTILFGKKTAGGGSTISQQLAKNLFPRDTSGRKNALVRTTKLVLS
                     KFKEWVTAVKLERNYTKEEILAMYLNVVEFGSNSYGIKSAAKTFFNTTPDSLHVEQAA
                     LLVGLVNAPTRYSPLRNPDKSLQRRNLVLQQMAKYGYIDRNQLDSLVAIPITLNYMEQ
                     DHNAGLATYFREMLRQFMTARKPERRFYYSQDMYSQDSLLWETNALFGWCSKNLKPDG
                     TPHNIYRDGLKIYTTINSKMQEYAEKSLTDHLKFELQPQLDREIKSRGGVLFSSQITR
                     EQANQIMYSAMRQTERYRLLRNAGASNESIIENFNTPVPMRVFSWRGERDTVLSPLDS
                     "
     gene            1533..1715
                     /locus_tag="PLX53_01615"
                     /pseudo
     CDS             1533..1715
                     /locus_tag="PLX53_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1971..3008)
                     /locus_tag="PLX53_01620"
     CDS             complement(1971..3008)
                     /locus_tag="PLX53_01620"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOY71809.1"
                     /translation="MRKLLIILPILLACAASTKVKAQNIPATRHRVMFYNVENLFDPF
                     DDSLTLDEEFTPFGDRHWTWKKMEQKLNGIYKVILAVGEYDPPVLVGLCEVENGFVLH
                     QLVSQTPLSKFEYRVVHRESPDRRGIDVALLYRPDRFTLQEKEFVTVRFPDEPWRTTR
                     EILYVRGLLGGDTLHVFVNHWPSKYGGELESEGGRYAAAYALKNKVDSIKIFYPDARI
                     LIMGDMNDEPESDPVARGFGACLTAETACPYDFVNLSAILKQRGQYSYKYQGVWGIID
                     QLIASRSMLDSRHVLYTFTGNAGVFSAPFLLEDDERYAGKRPFRTFIGFKYHGGYSDH
                     LPVYLDLFSNK"
     gene            complement(3010..4455)
                     /locus_tag="PLX53_01625"
     CDS             complement(3010..4455)
                     /locus_tag="PLX53_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71810.1"
                     /translation="MAKLPTACLLAVASIFALCHSACVRDDFSESSALKLSFSADTVL
                     FDTVFTTIGSSTQYFKVYNRSGHDLHISSIRLAGGSTSPYRINVDGRSGVTFSNVSLR
                     SRDSLFVFVEVTVDPTSLNSPLLVADSIMFSTNGNIQDVKLVAWGQDVHLLRSEIISN
                     DTVLATDKPYLIYSYLLVDTATTLTIPAGVQMYFHNQAMLVVAGTLKVEGEQGSPVVM
                     EGDRLEDFYRDKAGQWGGIWLTAGSRYSAISWAEIRNAITGIMVDTCFTSDAPTLTLS
                     HTRIENCSYVGLLARGAKVKADNCLVSNAAQVCVALTMGGEYRFYHCTVANYWGDYIY
                     RKGPALLLNNYYLYRLEENGPLYVEPRDLVEASFFNSIVYGSMSSEFQVDNIVNGAPV
                     AAQMNYHFEDCILKVPQNFDLTDTEKFTRVFTLDPKFKDSNAHVFLLDTLSPAMGIAN
                     PAVATDYPIDLNGFNRLTDGKPDLGAYERQE"
     gene            4590..>4904
                     /locus_tag="PLX53_01630"
     CDS             4590..>4904
                     /locus_tag="PLX53_01630"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOY71811.1"
                     /translation="MAKNWYVYLLLTVTLMACSKREKADLIVTNAKIYTVDSTFSTHS
                     SMAVVNGIVKELGDSVYIMSKYTAKKVVDAGGMYVYPGFIDSHCHFLGYGLSLNHAVL
                     SGA"
BASE COUNT         1306 a         1174 c         1180 g         1244 t
ORIGIN      
        1 tggagtcgag aggtgataaa accgtatcgc gctcaccccg ccatgagaaa accctcatcg
       61 gaacgggggt gttgaagttt tcgataatac tctcgtttga agctccggca ttacgaagca
      121 gcctataacg ctcggtctgc ctcatggctg agtacataat ttggttggcc tgctcacggg
      181 taatctgtga ggaaaacaga accccacccc tactttttat ttcacgatcg agctggggtt
      241 gcagctcaaa cttcaggtgg tcggtaagcg acttttctgc gtactcctgc attttggagt
      301 tgatggtggt gtaaatttta aggccatcac ggtagatgtt gtggggtgtt ccatcgggct
      361 taaggttttt ggagcaccat ccgaaaaggg cattggtttc ccaaagcagg gaatcctggc
      421 tgtacatatc ctgagagtag taaaatcgcc tttcgggttt acgggcggtc ataaactgcc
      481 tgagcatttc cctgaagtac gtcgccaaac ccgcattgtg gtcctgctcc atgtagttca
      541 aggtgattgg aattgcaacc agggaatcga gctggttgcg atcaatgtac ccatactttg
      601 ccatctgctg cagcaccagg tttcgtcgct gtaacgactt atcgggattc cgcagggggc
      661 tataccgggt tggagcattt accaatccca ccagcagggc tgcttgctca acatgaagag
      721 aatcaggagt ggtgttgaaa aatgttttag ctgccgattt aatcccatag gagttgctgc
      781 caaactcaac cacgttcagg tacatggcca gaatttcctc cttggtgtag tttcgctcca
      841 gcttaacggc cgtaacccac tccttaaact tcgacaatac cagcttggta gttcttacca
      901 gcgcattttt tcgccccgag gtgtcgcggg ggaacaagtt tttggcaagc tgctggctaa
      961 tagtgcttcc ccctccggcg gtctttttcc caaatagaat ggttttaacc atcacccttc
     1021 ccaaaccctt tgcatctatt ccgctatggg agtagaaacg gttatcctcg gttgcaatca
     1081 gcgcatggat tacattaggt gatatttcgt cgtagccgac aaacgtcctg ttttgaacgt
     1141 agaaattacc cagcaccacg ttatcctcgg atatgatttc cgaggcaatg ttgcttttcg
     1201 gattctccag ctcctcgaat gtgggcatgg gaccaaaaaa ttcgaggttg atgagtatga
     1261 aaatcaccag gaaaaaaagt agcaggaatg agataattcc ccaaaaccac catttaaact
     1321 ttcggattac ctccggattt ttccaattca tgggcttaaa aatttagcat cacgcgctcc
     1381 ctataaacat aaactttaac cattcgcccc ttaggtcaac tttcctttgt gccagcaagt
     1441 gaaaacgtgc aacgttcacc tggtagtaaa acgaacaaag ttaaaaacta ttttggacat
     1501 agctcacaac caggcaaccg gtggtaagga ttgataggct gatttcgaaa acaagagtgg
     1561 agcatgtttt cggttttatg gaacaaagta tgaatggtat aacggtacgt tctgttggta
     1621 ttgtcagagc gacaggcatt atcgggctaa taaatttgac atataattta ttccgatacg
     1681 aacaagttgt tcggttaaat attttacaaa gttaagtagt tgatagatag atagaaaaaa
     1741 gtttgcgaaa aaacgaaccg agcttgcgag ctcaacgaag ttaattttta gaacccaccc
     1801 taaaatactg cattcaacaa agtgttaatt ttatttgggc tgattccgtt tgcaaggttc
     1861 cccactttta ctaactttat tccgggtgat gacaacgctg gaggtaaccc ccaaacaaaa
     1921 tcaacgctcg gccgttatgg acaacggagg ctctccacat gaaaaacgcg ctacttatta
     1981 ctgaacaaat cgaggtaaac cggtaaatgg tcgctgtagc caccgtggta cttaaaccca
     2041 ataaaagttc gaaatggtcg tttgccggca taccgctcgt cgtcctcaag caaaaagggt
     2101 gcgctgaaaa ctcccgcatt gccggtaaag gtgtacagca catgccggct atccagcatg
     2161 gagcgtgacg caattagctg gtctattatg ccccacactc cctggtactt gtagctgtac
     2221 tgtccacgct gtttcagaat tgccgacagg ttaacaaaat cgtaaggaca ggcagtttcg
     2281 gctgtgaggc aagcgccaaa tccccttgcc acgggatccg attcgggctc atcgttcatg
     2341 tcgcccatga tgaggatacg ggcatcgggg tagaaaattt ttatggagtc aaccttattt
     2401 ttaagggcat aggcagcagc atagcgaccc ccctctgact ccagctcccc gccgtacttc
     2461 gatggccagt ggttaacgaa aacatgtagc gtgtcgcctc ctagaagccc acggacgtaa
     2521 agaatttccc gggttgtgcg ccagggttca tccggaaacc tgaccgttac aaactccttc
     2581 tcctgaaggg tgaacctatc gggacggtag agcagcgcta catcaattcc ccgacggtcg
     2641 ggcgactcgc gatgcaccac ccggtactcg aattttgaaa gcggcgtttg gcttaccagc
     2701 tggtgcagca caaagccatt ctccacctcg caaagaccta ccaaaacagg cggatcgtac
     2761 tcgccaacag ccaaaatcac cttgtaaatc ccgtttagct tctgctccat cttcttccat
     2821 gtccagtgcc tatcgccgaa tggtgtaaac tcttcgtcga gggtaagcga gtcatcgaat
     2881 ggatcgaaga ggttttcgac gttgtagaac atcacgcgat ggcgcgtggc agggatattc
     2941 tgagctttaa cctttgttga ggcagcacaa gcaagcaaaa tgggcagaat tatcagaagc
     3001 ttcctcatac tactcctgtc tttcgtatgc accaaggtcg ggtttaccat cggtaagccg
     3061 gttgaaccca tttaggtcaa tagggtagtc ggtagcaaca gcagggttgg caattcccat
     3121 ggcggggcta agcgtgtcga gcagaaaaac atgggcattt gaatccttga acttggggtc
     3181 gagcgtaaat acccgggtaa atttttcggt atcggtgagg tcgaagtttt ggggaacctt
     3241 caggatgcaa tcttcaaaat ggtagttcat ttgtgcggca accggtgcac cgttaactat
     3301 gttgtcaacc tgaaattcac tgctcatcga cccatacaca atgctgttga aaaatgaagc
     3361 ctccaccaaa tcacgcggtt caacgtaaag cgggccgttc tcctcaagtc ggtaaaggta
     3421 gtagttgttt agcagcagcg caggcccttt gcggtagatg taatcgcccc agtagttggc
     3481 aacagtgcag tggtagaagc ggtattctcc gcccatggta agggccacac aaacttgcgc
     3541 agcgttcgac accagacagt tatcggcctt tacttttgcc ccacgtgcca gcaggcccac
     3601 atagctgcaa ttttcaattc tggtgtggct aagagttaaa gttggcgcat cgctggtaaa
     3661 acaggtatca accataatac ccgttatggc atttctaatc tctgcccagc tgatagcgct
     3721 ataccggctt ccggccgtaa gccaaatccc tccccactgg ccggccttat cgcggtaaaa
     3781 atcctcaagc ctgtcgccct ccattacaac gggactacct tgctcgccct caacttttag
     3841 tgttccggcc acaaccagca tggcctggtt atggaagtac atctgtaccc cggcgggaat
     3901 ggtgagggtg gtagcggtat ccaccaaaag gtagctatag atcaggtagg gcttgtcggt
     3961 agccagcacc gtatcgttgc taatgatctc tgacctgagt aggtggacat cctgacccca
     4021 cgccaccagc ttcacatcct gaatattgcc attggtagaa aacatgatgg agtcggcaac
     4081 aagcaacggc gagttcaggg acgtagggtc aaccgttacc tccacaaaaa cgaaaaggct
     4141 gtcgcgggag cgtagcgaaa cattgctgaa ggttacgccc gaacgaccgt caacgttaat
     4201 ccggtagggc gaagtgctgc ctccggcaag gcgaatagac gaaatatgaa ggtcgtgccc
     4261 cgaccggttg tacaccttaa aatactgcgt tgaagagcct atagtggtaa atacggtgtc
     4321 gaatagaacc gtatcggccg agaagctgag tttcaaggct gagctttccg aaaaatcgtc
     4381 ccgcacacat gccgaatggc ataaggcgaa aatagatgct actgctaaaa ggcaggctgt
     4441 tggtaacttt gccattacta atgtctattt caacattcag agattacaat gtaacaaatt
     4501 tacaaaagct gttgagggta ttaaaacaaa gactaaattt gatagctgtg gctggttaaa
     4561 taatggccta aaaatcaata catacaaaga tggcaaaaaa ttggtatgtt tacctattac
     4621 taacggttac cctcatggca tgtagcaaac gtgaaaaggc cgacttaatt gttaccaacg
     4681 ctaaaattta caccgttgat tcaactttca gcacccatag cagcatggca gtggttaatg
     4741 gaatagtaaa ggagcttggc gattccgttt acatcatgtc gaagtacact gccaaaaaag
     4801 tagttgatgc cggaggaatg tatgtttacc ctggctttat tgactcgcac tgccattttc
     4861 tgggttacgg gttaagcctg aaccatgcag tgctttcggg ggct
//
LOCUS       DAOOQX010000058         4856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_20878_length_4856_cov_3.335972, whole genome shotgun sequence.
ACCESSION   DAOOQX010000058 DAOOQX010000000
VERSION     DAOOQX010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20878_length_4856_cov_3.335972"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PLX53_01635"
     CDS             complement(<1..828)
                     /locus_tag="PLX53_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOY71812.1"
                     /translation="MRHIGYFLLAATAALALPSSSNAVGLKNCHSGNGSAEKKIQETW
                     ADSVLSSLTLRERIGQLFMVAAYSNRDDMHVREIADLVRKDGVGGLIFFQGGPVRQAN
                     LTNYYQSLAEVPLLVGMDAEWGLGMRLDSTFSYPRQMMLGAAQDSALVYEMAADIARQ
                     LKRMGVHINFAPVVDINSNPQNPVINTRSFGENVNLVSRYGKAYVSGLQDNGVMACAK
                     HFPGHGDTFEDSHFSLPKVPHSKQQLDSVELYPFKQLISYGVQSVMVGHLRVPAMEND
                     "
     gene            1127..4396
                     /locus_tag="PLX53_01640"
     CDS             1127..4396
                     /locus_tag="PLX53_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOY71813.1"
                     /translation="MKKVCSMMLLLGLLSVGLAGAQEARLMRFPAVHGNQVVFTYAGD
                     LYTVPLNGGVARKLTSHIGYEMFARFSPDGKTIAFTGQYDGNTEVFTIPAEGGTPKRL
                     TYTATLSRDDISDRMGPNNIVMTWTPDGKNIVYRSRKQSYNDFVGSLLMVPIDGGLSS
                     ELPLTGGGFCSYSPDGKQLAFNWVFREFRTWKYYKGGMADDIRIFDFDTKQITNITNT
                     NTQEIFPMWIGGKIFFLSDRDRTMNLFCYDLKTQETKKVTNFTDYDIKFPSFSGNTIV
                     FEKGGYLYSLDASSLAANKIDVTIADDQLYSRSELVDAGKSIRSMDLSPNGERVVFSA
                     RGDVFTLPAESGITRNLTQSSDAHDRNAAWSPDGKYIAYISDKSGEYEIYLQAQDGAS
                     APVQLTTNTNNYIFNIEWSPDSKKILFTDRLSRLQCVDVETKAVSQLAKGKYGSIYSY
                     SWSPDSKWVAYSTEDENRFSVVFLYNTETASTYNVTGNWYTSTNPVFSNDGLYLLFVS
                     DRDFNPIYSSTEWNHAYTDMSKIFMVMLSKNTPSPFAPENNEVKIEPKPVTDAKKKNA
                     DDKKATEAQPTVKPVQIDIDGIVDRTVSLPIDAANYRVAACIDGKVYYSYYGSGRGSQ
                     VARVYDLKKKTETDLGESLQFAISANGKKMLVSKAGKYSVIDLPSSKVNMEKTISLNS
                     MKVWVDYRQEWKQIFDESWRQMRDFFYVENMHGLDWKAIHDKYAVLVPYVNHRNDLTY
                     IIGEMIGELSIGHAYVNSGVKPEPERTNTGLLGAKVSKDASGYFKIDRILEGASWDKS
                     LRSPLADIGVNVKTGDYILAVDGADLKNTPDIYAKLIDMADKTVELTVNSKPELNGSR
                     KVLVVPVANEANLYYYSWVQNNIRKVSEATNGQVGYIHIPDMGVEGLNEFVKYFYPQL
                     TKKALIIDDRGNGGGNVSPMILERLSRVAYRQTMRRGISYASPIPSETHVGPKVLLID
                     RYSASDGDLFPYGFKKLKLGPVIGVRTWGGVVGISGSLPFVDGADLRKPEFASFSSDA
                     SEWIIEGHGVDPDIVVDNDPYQEYMGVDTQLNKAIEVALDKLKDFKELPPIPPAPDKS
                     K"
     gene            4643..>4856
                     /locus_tag="PLX53_01645"
     CDS             4643..>4856
                     /locus_tag="PLX53_01645"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133345.1"
                     /note="catalyzes the release of the N-terminal amino acid
                     from a tripeptide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOY71814.1"
                     /translation="MINKQSLIDRFLRYVAIDTQSDDTVSDRFPSTEKQLVLSNLLVK
                     ELKELGVSDATIDEFGYVMGTIPANST"
BASE COUNT         1354 a         1199 c         1122 g         1181 t
ORIGIN      
        1 gtcattctcc atagcgggta cccttaggtg cccaaccata acgctttgta ctccgtagct
       61 gataagctgt ttgaatgggt aaagttcaac tgagtcgagc tgctgcttgc tgtgaggaac
      121 ctttggcagg gaaaagtgtg aatcttcaaa ggtatctcca tgcccaggga aatgcttggc
      181 acatgccata accccattat cctgtaatcc ggacacgtag gctttgccat agcggctaac
      241 caggttaacg ttttctccaa acgaacgggt attgataacc ggattttgtg gattgctgtt
      301 gatatcaaca accggtgcaa agttgatgtg aacacccatt cgtttcagct gccgggcaat
      361 atcggcagcc atttcgtaaa ccaaagcgga atcctgagca gccccaagca tcatctggcg
      421 gggataggag aaggtgctat cgagccgcat gccaagcccc cattcagcat ccatgcccac
      481 aagtagcggc acctcggcta atgactggta gtagttagta agattggcct gccttaccgg
      541 ccctccctga aaaaaaatca acccgccaac tccatccttc ctaaccaaat cggcaatttc
      601 cctaacatgc atgtcgtcgc ggttggagta tgcagccacc ataaatagct ggcctattcg
      661 ctccctcagc gtaagcgatg acaaaaccga atcagcccat gtctcctgta tttttttttc
      721 tgcagaccca ttgcctgaat ggcaattttt caaaccaacg gcattactac tgctcggcaa
      781 tgccaacgct gcggttgctg ctaaaagaaa ataaccgata tgcctcatca cctagtattt
      841 atgatgcaac aaaagtagca ggttttacgt cattttcaca gggacaagca gtgcatgaaa
      901 acattttgct ttagggtttt gaaatctgca aaaattttag gtacttgtaa agtaattcct
      961 gctgagagat ttacatttct aaatttaaag gtttcgcact gcccgacgtt agttagtcat
     1021 actatcaaag ctttgagatg agctgattaa accaaacaat cattccccta tcgaacatga
     1081 gtaattacag gaacacaacc ataacttcaa ctaaaataat ttcaagatga aaaaagtttg
     1141 cagcatgatg cttcttttgg gtttgctgtc cgttggatta gcaggtgccc aggaggccag
     1201 gttaatgcgt tttccggcag tccacggaaa tcaggtggtg ttcacctatg ctggcgattt
     1261 atatactgtg ccactcaatg gcggtgtggc tcgcaagcta accagccaca ttggctacga
     1321 gatgtttgca cgtttctcgc ccgatggaaa aacaattgct ttcaccgggc agtacgatgg
     1381 taacaccgag gtatttacca ttcctgccga aggtggaaca cctaaaaggc ttacttatac
     1441 ggcaaccctt tcgcgtgatg acatttcaga ccgtatgggc cccaacaaca tcgtaatgac
     1501 atggacaccc gacggtaaaa acattgtgta ccgttcccga aaacaatcct acaacgattt
     1561 tgtcggttcg ctattaatgg ttcccattga tggcggtttg tccagtgagc tacccctcac
     1621 cggaggggga ttctgctcct actcgcccga cggaaaacaa cttgccttta actgggtttt
     1681 tcgcgaattc cgaacatgga agtactacaa aggtggaatg gccgacgata tccgaatttt
     1741 tgatttcgat accaagcaaa tcaccaacat caccaacacc aacacccagg agattttccc
     1801 catgtggatt ggcggtaaaa ttttcttcct ctccgacagg gacagaacca tgaacctgtt
     1861 ctgctacgac ctcaaaaccc aagagaccaa aaaggtaacc aactttaccg actacgatat
     1921 caaatttccg tcattctcag gcaacaccat cgtttttgaa aaggggggat acctttactc
     1981 ccttgatgcc agttccctgg cagccaataa gattgacgtg actattgccg acgatcagct
     2041 ttacagccgc agcgagctgg tggatgccgg caagagcatt cgcagcatgg atttgtcgcc
     2101 aaacggcgag cgcgtggtat tctcggccag aggtgatgtg ttcacacttc ccgccgagag
     2161 cggaataacc cgcaacctta ctcaaagctc cgacgcccac gaccgcaatg cagcttggtc
     2221 gcccgacgga aagtatattg cctacatctc cgataaaagc ggtgagtacg aaatctatct
     2281 ccaggcacag gatggagcct cggctccggt gcagcttaca accaacacca ataactacat
     2341 attcaacatt gagtggtcgc ccgatagcaa aaaaattctt ttcaccgaca ggctctcaag
     2401 gttgcagtgc gttgatgttg aaaccaaggc agttagccaa cttgctaagg ggaaatatgg
     2461 ctcaatatac agctactcgt ggtcgcccga cagcaaatgg gtagcctact ccaccgagga
     2521 tgaaaatcgc ttcagcgtgg tgttcctcta taacaccgaa acggcttcaa cctacaacgt
     2581 tacgggcaac tggtacacct caaccaatcc tgtcttctcc aacgatgggc tttacctcct
     2641 ttttgtttcg gacagggact tcaacccaat atatagctcc acggaatgga accacgccta
     2701 caccgacatg agcaaaattt tcatggtcat gctctcaaag aacacaccct caccctttgc
     2761 tcccgaaaac aacgaggtga agattgagcc aaaaccggtt accgatgcaa agaaaaaaaa
     2821 tgccgacgat aaaaaggcta ccgaagcaca gcccacggta aaacctgttc aaattgatat
     2881 agacgggata gttgaccgaa ccgtatcact ccctattgat gctgcaaact acagagttgc
     2941 cgcatgtatt gacgggaagg tttactacag ctactacggt tcgggcaggg gttcgcaggt
     3001 ggcgagggta tatgacttaa agaagaaaac cgaaaccgac ctgggcgaat cgcttcagtt
     3061 tgccatatcg gccaatggaa aaaagatgct ggtatccaag gccggaaagt acagcgttat
     3121 cgatttaccc tcttcgaagg tgaatatgga gaaaaccata agcctcaaca gcatgaaggt
     3181 gtgggttgac taccgtcagg agtggaagca gattttcgac gagagctggc gtcagatgcg
     3241 cgatttcttc tacgttgaaa acatgcacgg gctggattgg aaagccatac acgataagta
     3301 tgccgtgctg gttccatatg taaaccatcg caacgactta acctacatca ttggcgagat
     3361 gattggggag ctgagcattg gccatgccta tgtaaatagc ggggtaaaac ctgagcccga
     3421 gcgcaccaat accgggttgc ttggcgccaa agtgagcaag gatgccagcg ggtacttcaa
     3481 aatagataga attcttgagg gtgccagctg ggacaagtcc cttcgttcgc ccctggccga
     3541 tatcggcgtt aatgttaaaa caggcgacta cattctggca gttgacggag ccgacctgaa
     3601 gaataccccc gacatctacg caaaactgat tgacatggcc gataaaacgg ttgagctgac
     3661 ggtgaacagc aaacccgagt tgaacggaag ccggaaggtg ttggttgttc ccgtggcaaa
     3721 cgaggccaac ctgtactact acagctgggt tcagaacaat attcgcaagg tatccgaggc
     3781 caccaatggc caggttggct acatccacat tcccgacatg ggcgttgagg ggcttaacga
     3841 gtttgtaaag tacttctacc cccagctaac caagaaagcg ctgattattg acgacagggg
     3901 taacggcgga ggtaatgttt cgcccatgat tcttgaacgt cttagccgtg ttgcctaccg
     3961 gcaaaccatg cggcggggta taagctacgc ctcgcccatc ccatccgaaa cacatgttgg
     4021 gcctaaggtg ctgctcatcg accgctactc agcttccgat ggcgatttat tcccatacgg
     4081 atttaaaaag ctgaaactcg gcccggttat tggggttcga acctggggcg gagtggttgg
     4141 tatttcaggc tcacttccat ttgttgacgg cgccgacctg cgtaaaccgg aatttgcaag
     4201 ctttagcagc gatgctagcg agtggattat cgagggacat ggtgttgacc ccgatattgt
     4261 ggttgacaac gacccctacc aggagtacat gggcgttgat acccagctga ataaggccat
     4321 tgaggttgca cttgataagt tgaaggactt taaggagctg ccaccaattc caccagcacc
     4381 cgataaatcg aagtagccgg aacaacaaga tacaaaacta cagagcggcc atggcttaac
     4441 atgagccgct ttgttttttt aactactatc aacttccagc atggctataa ccttctattt
     4501 atcaacctac tagcataaac aagtaaatta ttttcatttt tttatgcata gttgtcccaa
     4561 agaacctaaa tttaaaggct attttttcta agacgtcatt atttttctaa aaaacaaatt
     4621 tgctttttaa catactcaag ccatgataaa taagcagtcc ctgatagacc gttttttaag
     4681 gtacgtagct attgatacgc aaagcgacga tacggtatcc gatcggtttc caagcactga
     4741 aaagcagctg gttctatcaa atttgctggt gaaggagtta aaggagctgg gcgtatccga
     4801 tgcaacaatc gacgagtttg gctacgttat gggcaccatt cctgccaatt caacaa
//
LOCUS       DAOOQX010000059         4841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_20960_length_4841_cov_3.585875, whole genome shotgun sequence.
ACCESSION   DAOOQX010000059 DAOOQX010000000
VERSION     DAOOQX010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20960_length_4841_cov_3.585875"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="PLX53_01650"
     CDS             complement(<1..1724)
                     /locus_tag="PLX53_01650"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOY71815.1"
                     /translation="MYKLLPPFCIAILLFAVQPAQAFLDEDKKICQAVRVNPKPPVVD
                     GSLDDEVWEKGEWSGNFIQHEPYNGNEPSQKSYFKILYDSDYLYAAFKMSDSEPDKVV
                     SRITRRDDADGDRVTLCIDSYFDQRTAFLFTTNAAGVKSDEVMTENGDKEDMNWDPIW
                     WAKAVKTPDGWTAEMKIPLNQLRFANSSDMKWGVQVMRYIYRNQETDLWQHVPKDAPG
                     FVHLFGELHGLNGLAPKRQVEVAPYMVTKTERLEAESGNPFMTGKNNGFNAGVDAKIG
                     ITNNFTLDLTVNPDFGQVEADPAVVNLSAFETYFEEKRPFFIEGSGMMNFSLMFGDGD
                     LATQNLFYSRRIGRAPQLQPDLDDNEYAKIPSNTAILGAAKVTGRTGKGMSVGILETI
                     TNSTYATIDSMGVRTHEIAEPLTNYTVASVRQEFSGGNTIISGIATSTNRQLNDLSSD
                     YFHQNAFTGGMDLQRFWSNKKYLFCGKLYFSHVNGSKEAIARTQTSPARYFQRPDNNY
                     ARLDSTRTSLTGTGGLLQFGRVGEGHLQYMGAVTWHSPQLEINDIGYIQNVDDIFQVM
                     WVGLRFWKP"
     gene            complement(1980..2561)
                     /locus_tag="PLX53_01655"
     CDS             complement(1980..2561)
                     /locus_tag="PLX53_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOY71816.1"
                     /translation="MNNDIILAGVGGQGILSIAAAIGLAAVNSNLHIKQSEVHGMSQR
                     GGDVQSHLRISDAPIASDLIPAGKAHLIISVEPMEGLRYMPFLSKNGWLVTNITPFDN
                     IPDYPKVDEILKRVKAVKNHIALDADSIAKECGSTKASNIVMLGAASPFIDIPAAGIE
                     EGIRQLFNRKGEDIVNLNLKAFRAGREYSEKNR"
     gene            complement(2671..4281)
                     /locus_tag="PLX53_01660"
     CDS             complement(2671..4281)
                     /locus_tag="PLX53_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOY71817.1"
                     /translation="MEKLLLLGDEAIAQGAIDGGLSGIYAYPGTPSTEIMEYVQGSKM
                     AAERNIHRQWSANEKTAMEAAIGMSYAGKRAMVCFKHVGLNVAADAFINSAITGVNGG
                     LVVVSADDPSMHSSQNEQDSRFYGKFAMIPILEPSNQQEAYDMAYQSFELSERLNVPV
                     LFRITTRMAHSRADISRHEVKDQNKLRMPLDPRQYVLLPAIARKNYKKLLSTQPAIEL
                     EAEQSKFNAFIPGENHDMGIVATGIAYNYLVENYPNHQCPFPVVRIGHYPIPRKKITE
                     LYESCKSLLVIEEGYPMVEEMLRGFFNVGKPIHGRLDGTLPRDGELNPNIVAKALGFP
                     AAGEEPLAEVVKNRPPSLCMGCGHRDVYTDLNEAIKDYSAGRVFSDIGCYTLGALPPF
                     NAINSCVDMGASITMAKGAADAGLVPAVAVIGDSTFTHSGMTGLLDAVNENSPITIII
                     SDNSTAGMTGGQDSSATGKIVDICRGMGVDPSHIRVVVPLKKNHEENVKIIREELAYN
                     GVSVIIPQRECIQTFARRKKTEKKESND"
     gene            complement(4580..>4841)
                     /locus_tag="PLX53_01665"
     CDS             complement(4580..>4841)
                     /locus_tag="PLX53_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOY71818.1"
                     /translation="NENADYTVRKDFQKFISTLVPFSNTYEHTLEGEDDMTAHIKSSL
                     LGQSVWIPISNGKLNLGTWQGIYLCEFRNHGGNRKLVITIIS"
BASE COUNT         1187 a         1105 c         1170 g         1379 t
ORIGIN      
        1 ggtttccaga acctaagtcc aacccacatc acctgaaaga tatcatcaac gttctggatg
       61 tatcctatat cgttaatttc gagttggggg gagtgccagg taaccgctcc catgtactgc
      121 aggtgtcctt cgccaaccct tccaaactgt agcaatcccc cagtaccggt aagcgacgtg
      181 cgagtagagt cgagccgggc atagttgttg tctggccttt gaaagtagcg agctggagaa
      241 gtttgagttc tggctatagc ctccttgctt ccgttaacat ggctaaaata gagttttcca
      301 caaaacaggt atttcttatt cgaccaaaat cgctgtaaat ccatgccgcc ggtaaaggca
      361 ttttgatgaa agtagtcgct ggagagatcg ttcagctggc ggttggttga agtagctatg
      421 cccgaaatga tggtgtttcc tccgctaaac tcctgccgca cgctggcaac tgtgtagttg
      481 gtgaggggtt ccgctatttc atgtgtgcga acacccatac tgtcgatggt ggcgtaggtg
      541 ctgttggtaa tagtttcaag aataccaacc gacatgcctt ttccggttcg tccggtaacc
      601 ttggctgcgc cgaggattgc agtgtttgaa ggaattttag catactcgtt atcgtccaaa
      661 tcgggttgaa gctggggagc gcgaccaatt ctccgggagt aaaacaggtt ctgggttgcc
      721 aggtcgccat cgccaaacat caacgaaaag ttcatcatgc cgcttccctc aataaaaaat
      781 ggccttttct cctcaaagta ggtttcgaaa gccgaaagat ttaccacggc agggtcggct
      841 tcaacctgcc caaaatcggg gttaacggtt aggtcgagtg tgaagttatt ggttattcct
      901 atttttgcgt caacgccagc attgaaacca ttatttttac cggtcataaa gggatttcca
      961 gattcagcct caagtcgttc ggttttggta accatgtagg gtgctacctc cacctgccgc
     1021 ttgggggcaa gtccgtttag cccgtgcagc tcgccgaaaa ggtgaacaaa tccaggtgca
     1081 tcctttggaa catgctgcca gaggtcagtt tcctggttgc ggtaaatgta ccgcatcacc
     1141 tgaacgcccc atttcatgtc ggatgagtta gcgaaacgaa gctggtttaa cggtattttc
     1201 atttcggctg tccagccatc gggagtttta accgctttgg cccaccagat gggatcccag
     1261 ttcatatctt ccttatcacc attttcggtc atcacctcat cgcttttcac tcctgcagcg
     1321 ttggttgtaa agaggaatgc agtacgttgg tcaaagtagg agtcgatgca gagtgtaacc
     1381 ctatccccgt cggcatcatc gcgccgggtt atcctcgata caaccttatc gggctcgcta
     1441 tcgctcatct taaaggcagc atacaggtag tcgctatcgt atagaatttt gaagtaggat
     1501 ttctggctgg gctcattccc gttgtagggc tcatgttgga taaaatttcc gctccattca
     1561 cccttttccc atacttcatc gtcaaggctt ccgtcaacaa cagggggttt tggatttacc
     1621 cggacggcct ggcaaatttt cttatcctca tcaaggaatg cctgggcagg ttgaacagca
     1681 aatagcagta ttgcaatgca gaatgggggc agcaacttat acatacttat gttttaaggt
     1741 tttcaataca acaacataac gacgacatgt tgtgagtttt gctgcaagcg ggatgaaaat
     1801 ttgctgtagg acgcaaaaat aatgacctgc ttacctttac caatgcgtaa aaaaaaaact
     1861 ccagccgtcg ctggagtttt tcgataatgt gtaatgtagc cgacctattt tctgagaaaa
     1921 cctgtaaccc tcatttccag tgggttaaac aacgtatact cttgcagggt aatagaggat
     1981 tagcggttct tttcgctgta ttctctgcca gccctgaagg ctttaaggtt aaggttgacg
     2041 atatcctctc ccttacggtt aaatagctgc cgtattccct cctctatgcc agcggcagga
     2101 atatcaataa aaggtgaggc tgcccctagc atgacgatgt ttgaagcctt tgttgacccg
     2161 cactccttgg caatggagtc ggcatcgagg gcaatatggt ttttaacggc tttcaccctc
     2221 tttagtatct cgtccacctt tgggtagtcg ggtatgttgt caaagggggt tatgttggta
     2281 accagccatc cgtttttcga cagaaatggc atgtagcgta agccttccat gggttcaacc
     2341 gagataatca ggtgggcttt tccggcggga attaagtcgg aggcaatggg tgcatcggaa
     2401 atgcgaaggt gcgattgcac atcacccccg cgttgactca taccgtgaac ctccgattgc
     2461 ttgatatgca ggttgctgtt aacagcggcc aggccaattg ccgcagcaat tgaaagaatt
     2521 ccctgtccgc caacacctgc taaaattata tcgttattca ttataaattg tttaactagt
     2581 tgatatataa tacgttgtta gaaatattga ttaattgctg agctacgttc atccgaaaaa
     2641 gatgcatggt aaactttctc cagctcattg ctaatcattt gattctttct tttctgtttt
     2701 tttcctacgg gcgaaagtct gaatgcactc acgctggggg attattacgg aaacgccatt
     2761 ataggctagc tcctcgcgaa ttatcttgac gttttcctcg tggtttttct ttaacggtac
     2821 aaccaccctg atgtggctgg ggtcaacgcc catacccctg caaatgtcaa caattttacc
     2881 tgttgccgaa gaatcctggc ctccggtcat accggcagtt gaattatcgg agatgatgat
     2941 ggttataggg gagttttcgt ttacagcatc gagtagccct gtcataccgc tgtgggtgaa
     3001 ggtggagtcg ccaatgacag caacagctgg aacaagtccg gcatcagctg cccctttggc
     3061 cattgtaatg gaggctccca tgtcaacgca tgaatttatg gcgttgaatg gtggcaaagc
     3121 tcccagggtg tagcacccaa tgtcggaaaa aacccggccg gcgctgtagt ccttaattgc
     3181 ctcgttcaaa tccgtgtaaa catcacggtg tccacatccc atgcagagcg atggagggcg
     3241 gttttttaca acttctgcca aaggctcttc accagcagcc ggaaaaccta atgccttggc
     3301 aactatgttt ggatttaact cgccgtcgcg gggcagggtc ccgtcgagcc gtccatgaat
     3361 tggcttgcca acattgaaaa atcccctaag catctcttcc accatggggt atccctcttc
     3421 aataaccagc agcgatttgc agctttcgta gagctcggta atttttttgc ggggaatagg
     3481 gtagtgtcca atcctgacaa ccggaaaggg acactggtgg ttggggtagt tttcaaccag
     3541 gtagttgtag gcaattccgg tagctacaat acccatgtcg tggttttctc ctggaatgaa
     3601 ggcgttaaat ttagattgtt cagcctccag ctcaatagca ggttgggtgg aaagaagctt
     3661 tttatagttt ttacgtgcta ttgcgggaag taaaacgtac tgtcttgggt cgagcggcat
     3721 tctaagcttg ttttgatcct taacttcatg cctgctaata tcggcgcggg agtgtgccat
     3781 ccgggttgta atcctgaaca gcacggggac gttaagcctt tcggagagtt caaaactttg
     3841 gtaggccatg tcgtaggcct cctgctggtt tgatggctca agaatgggaa tcatggcaaa
     3901 tttaccatag aaccttgagt cctgctcgtt ttgcgatgag tgcatggagg gatcgtcggc
     3961 cgatactacc accagccctc cattaacgcc tgttatggca gaattaataa aggcgtcggc
     4021 agccacgttg agccctacgt gcttaaaaca caccattgca cgcttgccag cataggacat
     4081 gccgatagca gcttccatgg ctgtcttttc gttggccgac cattgcctgt gaatgttccg
     4141 ttcggcagcc atttttgaac cctgaacata ctccatgatt tctgttgatg gggtgccagg
     4201 gtaggcgtaa atgcccgata gccctccgtc tattgctccc tgggcaatgg cttcatcgcc
     4261 tagaagtagt aacttttcca tttcgtttgg tttattcttc aaaattgtta tttggctttt
     4321 gctttggcgt ggttagctat taaggtaatg gtataagcta aacaagttct aaaggccacc
     4381 aaaacgcttc aggtaaccta cgttctgggc aacgttatat aagtaatacg ccgaatacat
     4441 ggtaaaatca tttcagtttt aaaccattca gttcaaaatt gggttctgat tgttagcaaa
     4501 agtaggaatt attttagaat gattaagcta aaagcccttt tttgatgaaa agttaaaatt
     4561 taaggtaggt aatgttacgt caggaaatga tagtaataac cagcttacgg tttcccccat
     4621 gatttcgaaa ctcgcatagg taaattccct gccaggtgcc aaggttcaac tttccgttag
     4681 agatgggaat ccaaaccgat tggcctagga gagacgattt aatatgggca gtcatatcgt
     4741 cttcaccttc cagggtatgc tcgtaggtgt tggaaaaagg gacaagggtg gatataaatt
     4801 tctgaaaatc ttttcgtacg gtgtaatcgg cattctcatt a
//
LOCUS       DAOOQX010000060         4797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_21188_length_4797_cov_2.823492, whole genome shotgun sequence.
ACCESSION   DAOOQX010000060 DAOOQX010000000
VERSION     DAOOQX010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21188_length_4797_cov_2.823492"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..1292
                     /locus_tag="PLX53_01670"
     CDS             240..1292
                     /locus_tag="PLX53_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71819.1"
                     /translation="MRGKLLMLLVASGVILSVSAYGQNNLPAYLGVSYIPELRWKVYF
                     INHKSIGEPLSLNFDMTSMSSYEGNFGIRRLGVRMGVSAQTENNLIGKFYRWGGYLGF
                     KNMLLRLQSSKMSGDLEWSGALPPGYYANREFSNKYFNIDLLKTAKGGSAFNEVGGYW
                     GVGYTSMGYPVEISTLVTEGGRENQKFGMPAYDTLYSIKSYNICFGFDLLRQLCLTGG
                     LEGFAPGRRAMRFAMYASTQDKVGFGPAKISDYGVSMAEALNPGRTAVTSKFFNVMVH
                     YSLSLGLRYYIHPGPTVVIFALGYDFEGAMNAPFKGAADNAEDLGFDFLNYYLNHGVS
                     FKIYLAWNRDWQQKNQ"
     gene            complement(1301..2335)
                     /locus_tag="PLX53_01675"
     CDS             complement(1301..2335)
                     /locus_tag="PLX53_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY71820.1"
                     /translation="MKKLLLLSPQLVLTLSLFGQTIITSQNHGFIPNDNNPMLLTSYV
                     EPGPAGKNVVWNFSMLETSENFQGSLKEVYTLKDYPSNQGGNVVLEEFGNRFIFNMTA
                     NSLEQVGFQSSTGSTEITYSKPFVKMRYPFGFNSSFSGDFKGDYLVNKVPVGTISGTY
                     RVEGDGYGKLILPGGRELSGVLRVKEVKLTTQTINNSLINIEDVTYRWYVANHRFPVL
                     SLIKSTYTPANGKPSTQTKAAFNGNVVSQPSNVDKVSDNLLRFTTFPNPYKGKVAITY
                     TLPESCKVNLAVYDITGKLVSVLVNGIEDAGQKTHYFSAAEVGMPAATYIVKLTAGKV
                     EFTGKVLEVR"
     gene            complement(2688..>4797)
                     /gene="recQ"
                     /locus_tag="PLX53_01680"
     CDS             complement(2688..>4797)
                     /gene="recQ"
                     /locus_tag="PLX53_01680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOY71821.1"
                     /translation="IHNVLTGNDTFVLMPTGGGKSLCYQLPALILDGTAIIISPLIAL
                     MKNQVDSMRAFVLEEGIAHFMNSSLNKTQLQKVRQDVTNGVTKMLYVAPESLTKEENI
                     QFLKQIKISFYAIDEAHCISEWGHDFRPEYRRIRPIINEIGTAPIIALTATATPKVQH
                     DIQKNLGMLNASVFKSSFNRPNLYYEVRPKVNAVKEIIKYIKSNQGKSGIIYCLSRKK
                     VEEMAEILKINGIKALPYHAGMDAATRSENQDKFLMEEVDVIVATIAFGMGIDKPDVR
                     FVIHYDIPKSLEGYYQETGRAGRDGGEGQCITFYSYKDIQKLEKFMQGKPVSEQEIGK
                     QLLLETVSYAESSMCRRKTLLNYFGEEYDEENCNSCDNCLHPKTQFEGKEELQMVLET
                     VLAVNQKFKADHIVNILLGNVTAAVKSYKHNQLDTFGEGAEKDSRFWNSIIRQALVMK
                     FLDKDIENYGLLFITPKGQEFIDKPTDVLFTLDHEYEEADDDESIGMPMDRSGAVDDE
                     LLAMLKDLRKKMAKKKNLPPFVIFQDPSLEDMAIQYPITIEELHSITGVGAGKAKRFG
                     QEFVELIKAYVEDKDIIRPQDMVVKSVATKSLLKVYIIQSIDRKMDLEDIANAKNLEM
                     DELLNEIEAIVNSGTKINLDYYINQVIDEDKQEEIFDYFRNEAETESLEEALVALGDE
                     DYTEEEIRLMRIKFLSEMGN"
BASE COUNT         1125 a         1074 c         1177 g         1421 t
ORIGIN      
        1 aatcttggga ttcaaatgaa tgaacttagc cattaaccct acctgttatc gtgcgaattt
       61 accaaaatat ggggtaggta aaatggatgt tgataactat tgaattttct tttcgcattc
      121 aggtttggtt acttcaattt ggctgtttgc cacattttta ctgtagtttg taaaaggagt
      181 tatggaatat atgaatctta cttctatttt tatagcttag catattaaat ttttatagga
      241 tgcgcggaaa actactaatg ctattggtgg ctagcggagt gatattatct gttagcgcat
      301 acgggcagaa taacctacct gcctatctag gtgtctcata cattccggag ctgagatgga
      361 aggtgtactt catcaaccat aaatctatcg gcgaacctct ttccctgaat tttgacatga
      421 ccagcatgtc gtcctacgag ggcaattttg gtattcgtag gctaggggtc aggatggggg
      481 tgtcggccca gacggagaac aacctaatag gtaagtttta ccgctggggc gggtacttgg
      541 gatttaagaa catgctacta cggctacaga gttccaagat gtcgggcgac ctggaatggt
      601 cgggagcgct gcctcctggt tactatgcca atagggaatt ctccaacaag tacttcaaca
      661 tagatctttt aaagactgct aaagggggtt cggcgtttaa cgaagttgga gggtactggg
      721 gtgttggcta tacctcaatg ggttatccgg tagagattag caccctggtt accgaaggcg
      781 gaagggagaa ccagaagttt ggaatgcctg cttatgatac gctctactcc attaaatcgt
      841 ataatatttg tttcggattt gacttgcttc gacagctttg ccttacagga ggacttgaag
      901 gttttgctcc gggaagacgc gccatgcgtt ttgccatgta tgcctccacg caggataagg
      961 ttggttttgg gccggccaaa atatccgatt acggagtttc tatggccgaa gcgcttaacc
     1021 cgggtagaac tgcagttacc agtaagtttt tcaatgtaat ggttcattac tccctctcct
     1081 tggggttgcg ctactacatt catcccgggc caacggtagt aatttttgct ctgggatacg
     1141 actttgaagg ggccatgaat gcgcccttta aaggagcagc cgataacgcc gaggatttag
     1201 gtttcgactt tttgaactac tacctgaacc atggtgtttc gtttaagata tacctggcat
     1261 ggaacaggga ctggcagcag aaaaatcagt aactacctgt ttacctcacc tcaagcacct
     1321 tgccagtgaa ttctaccttt cctgctgtaa gcttaacgat gtaggttgca gcaggcattc
     1381 ctacttctgc tgcagaaaag tagtgggttt tttgtcccgc atcctcaata ccgttcacca
     1441 aaacgctaac aagcttgccc gtaatgtcat acaccgcaag gtttactttg cagctttcgg
     1501 ggagggtgta ggtaatggca accttgccct tatagggatt tggaaatgtt gtaaacctca
     1561 gcaggttatc cgacaccttg tccacattgc ttggctgact tacaacattg ccgttgaagg
     1621 ctgccttggt ttgtgttgaa ggtttcccgt ttgccggagt gtaggtgctt tttattagcg
     1681 atagtaccgg aaaacggtgg ttggccacat accagcgata ggtaacatcc tcaatgttaa
     1741 tcaggctgtt gttgatggtt tgcgtggtga gcttcacctc cttaacccgt aaaactccac
     1801 ttagctctct tccaccgggt aaaatcagct taccgtagcc atcgccctcc acccggtagg
     1861 ttcctgaaat ggtgccaaca ggcactttgt taacaaggta atcaccctta aaatctccgg
     1921 aaaagctgct gttaaaacca aacgggtagc gcattttgac aaatggcttg ctgtatgtta
     1981 tttcggtgct tcctgtagat gactgaaaac caacttgctc cagtgagttg gctgtcatat
     2041 tgaagataaa cctattgccg aactcttcaa gcaccacgtt gccaccctgg tttgaggggt
     2101 aatccttgag ggtgtaaacc tcttttaggc tgccctggaa gttctccgaa gtttccagca
     2161 tgctgaagtt ccacaccacg tttttaccag ctgggccggg ttctacgtag cttgtaagca
     2221 gcattgggtt attgtcatta ggaatgaatc cgtggttttg gctggtaata atagtttgcc
     2281 cgaataaact tagggtaaga accagctgag ggctgagcag taataatttc ttcatgacgg
     2341 cctcctttta ggtttagcta tgcccaaata tagctcaact tcggaatgaa atcaatacgt
     2401 tgtgcggtta attttttgtt aacttcttta agggcagctc cataagcaaa gaggttgggt
     2461 ttagtgagtt gaaaagttaa cagaatgctt ttctcaggct ggtagaagag tcttggatgt
     2521 gaagcaatgt ggcgtttggt gatggtatgg caaaaaaagt tgtactttgg tgtgggtggt
     2581 cagcagcaac ggcaaacagc tatatctgcc cctgttacct ttggagcatg ccgcttacat
     2641 tgtgatttac aggtaaaatg aggttgtggg cgggaagttc gggccaatta gtttcccatt
     2701 tcggaaagga acttaatccg cataagcctg atttcctcct cggtgtaatc ctcatcgcca
     2761 agggcaacaa gagcttcctc gaggctttcg gtttcggcct cattgcggaa gtagtcgaat
     2821 atttcctcct gcttatcctc gtcaataacc tggttgatgt agtagtcaag gttgattttg
     2881 gtacccgagt tgacaattgc ctcaatttcg ttcagcagct catccatttc gagatttttt
     2941 gcgttggcta tgtcttcgag gtccattttc cggtcgatgc tctgaatgat gtataccttg
     3001 agaagcgatt tggtggcaac cgatttaacc accatgtcct ggggtcggat aatatcctta
     3061 tcctcaacgt atgccttaat cagctcaaca aactcctgtc cgaagcgctt ggcttttccg
     3121 gcgccaactc cggtaatact atgcagctcc tcaatggtaa tggggtactg gatggccata
     3181 tcctccagcg atgggtcttg aaagattaca aaggggggaa ggtttttctt tttggccatt
     3241 ttctttcgca ggtctttcag cattgcaagc agctcgtcat caacagcgcc cgacctgtcc
     3301 atgggcattc caatagattc atcgtcatcg gcttcctcgt actcgtggtc cagggtaaag
     3361 agcacgtcgg tgggtttgtc gataaattcc tgacccttag gcgtaataaa tagcagcccg
     3421 tagttttcga tgtccttgtc caggaacttc atcaccaggg cctgacggat tatggagttc
     3481 cagaaccggg agtccttttc ggcgccctcg ccgaaggtat cgagctggtt atgcttgtac
     3541 gatttaaccg ctgcggtaac gttgccaagc aggatgttga ctatgtggtc agctttaaac
     3601 ttttggttga ctgccagcac cgtttccagc accatttgaa gctcctcttt cccctcaaac
     3661 tgtgttttgg ggtgtaggca gttgtcgcac gagttgcagt tttcctcatc atactcctct
     3721 ccgaagtagt tcagaagagt tttccgacgg cacatggatg actcggcgta cgacacggtt
     3781 tcgagcagca gctgcttgcc aatttcctgt tccgacaccg gtttcccctg cataaacttt
     3841 tcaagttttt gaatgtcctt gtagctgtaa aaggttatgc attgaccttc gcccccatcc
     3901 cggccagccc gaccggtttc ctggtagtat ccctcaaggc tcttgggaat gtcgtagtgg
     3961 ataacaaacc taacgtcggg cttgtcgata cccattccaa aggctattgt tgccacaatt
     4021 acatcaacct cctccatcag gaatttatcc tgattttccg agcgggtggc ggcatccatc
     4081 cccgcatggt agggcaacgc cttaattccg ttgattttga gaatttcggc catctcctca
     4141 accttcttcc ggctaaggca gtaaatgatt cccgacttgc cctggttgga ctttatgtac
     4201 ttgatgattt ccttaactgc attcaccttg gggcgtacct cgtagtacag gttgggcctg
     4261 ttgaacgacg atttgaaaac actggcgttg agcatgccga ggtttttctg gatgtcgtgc
     4321 tgaaccttgg gggttgccgt agcagtcagg gcaattatag gagctgtgcc tatctcgtta
     4381 attatggggc gaattcttcg gtactccgga cggaagtcgt gcccccattc cgaaatacag
     4441 tgcgcctcgt caattgcgta gaatgaaatt ttgatttgct ttagaaactg tatgttttcc
     4501 tctttggtaa gcgattcggg tgctacatac agcattttgg taacaccgtt ggtaacatcc
     4561 tgtcttacct tttgaagttg ggttttatta agcgaggagt tcataaagtg ggcaatgccc
     4621 tcctccagaa caaatgcgcg cattgagtca acctggttct tcataagggc gatgaggggc
     4681 gatatgatga tggcggttcc atcaagtatt agggcgggca gctggtagca tagcgatttg
     4741 cctccaccgg tgggcataag aacaaacgtg tcgttccctg taagaacatt gtgaata
//
LOCUS       DAOOQX010000061         4730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_21570_length_4730_cov_4.155294, whole genome shotgun sequence.
ACCESSION   DAOOQX010000061 DAOOQX010000000
VERSION     DAOOQX010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21570_length_4730_cov_4.155294"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(63..153)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(404..1777)
                     /locus_tag="PLX53_01685"
     CDS             complement(404..1777)
                     /locus_tag="PLX53_01685"
                     /inference="COORDINATES: protein motif:HMM:NF018583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HOY71822.1"
                     /translation="MITVIIPTLNEQNTISQVIELVEKSANVSEILVVDDKSLDSTVK
                     IARAKNVKIYTSTKLGKGSSMYEGMLLATNGIIVYLDADITTYPENIVNLLTDPIINN
                     TADFVKATFNRQAGRVTELVAKPLLSLLFPELAHFSQPLSGMIAAKKSILEKATFEND
                     YGVDVGLLIDMFQEKARIVEVNIGYIENRMQSWEQLAKMSKEVSRAILKRAELIKHSN
                     LETLGNFAMIRDQMDFAIKESLLGLNKMLIFDMDNTILTKSFILTLATNYKFKEKLIE
                     IVAENSSPYVRTKLIAQLLKGLKINELLETLDGIPTVNDFERIVKEFKQKGYICGIIS
                     DSYDCITNHLVNKFDLDFSIANELEFSNGMATGEVKIPSAFLRTKDSTCNHDFCKSNA
                     MREVAHRYHIDLHNIIAVGDDENDICMIKDCGIGVAFCSSNSTLNFIADRVIQERTFA
                     PLIELLK"
     gene            complement(2275..2673)
                     /locus_tag="PLX53_01690"
     CDS             complement(2275..2673)
                     /locus_tag="PLX53_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71823.1"
                     /translation="MNNDFICPKCEGYLVVGGSVILSISMQSGLRGLILLSPELGNYA
                     KVSSPHVKIENGEMLDYYCPICHAKLAASDVHPRLVRLIMIDDKSKRHEIFFSGIVGE
                     HCTYKISETVYEKFGESSATYDQYFKTRRI"
     gene            complement(2868..>4730)
                     /locus_tag="PLX53_01695"
     CDS             complement(2868..>4730)
                     /locus_tag="PLX53_01695"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOY71824.1"
                     /translation="TIIDTRRTTDYINTFLSDYRFGGRLPVWALAANETECMIGYHSV
                     PVIADAWIKGIRNFDPDLALKAMLHSANLNHFGLKEYRQYGFIPGDMEHESVSKTLEY
                     AYDDWCIAQFAKSIADSSTYQKFIMRAQSYKNLFDPSTHFMRPRINGGWKDNFNPSEV
                     DQNFTEANSWQYSFYVPHDVSGLIELHGGKKNFIGRLDELFSTSSQLMGKNQVDITGL
                     IGQYAHGNEPSHHMAYLYCYAGQPWKTQKLVRKIMDEMYTSKPDGLCGNEDCGQMSAW
                     YILSAAGFYPVCPGSTQLVIGSPLFSKITFNLENGKYFSVVAENNSPENIYINSASLN
                     GKPITRCWIDYNEIMAGGELRLAMSPTPNSGWGSTDADMPTSSIDEHFIATAPAFNTS
                     TKTYMDSLQLTLTTVQPQASTFYSVAEINHPEAIPIWTQGNTLNLKSSKRVKAYSVLP
                     GGESSAIVEGSFIRIEGIKNVELKSIPSPLYDGGGKLALVDGQRGSTNFRLGGWQGYQ
                     NQDFEAIVDLGHERELHYLGAEFLQDASPWIMMPRQVTFSVSSDGTSYSNIGVVNNDI
                     PDTLEKPIVKELGVAVNVEARYVKVFAKSYGKLPSSHESAGESSWLFIDEIVIR"
BASE COUNT         1347 a          966 c          974 g         1443 t
ORIGIN      
        1 accccggttg atgctcttga gtagcctttt aaaaaagggg gaagtaaaaa aacttgagat
       61 tgatacctaa aactcttttt cccttcgccg gtattacccg gatcaggttc aaagggtgta
      121 atctcagcct taaaggcacc cccaaaagtc gggggtaaag gtaaccattt taaatgatgt
      181 gaaaaatttt ttaagggttt ggctaggcat gttatcagca gccgaactca aatgttccat
      241 tttgaggcta ttccatcggg attcggacta tgttgaaacg gagtgggtat attttgaaaa
      301 aggacatctt ttcaatatag gtagcaaaat gtctaaaagc ttatcctcct ttttagaatt
      361 tactttatta aattgataaa aagcaaagct taatatttat tttttatttc agaagctcta
      421 tcaaaggggc aaatgttctt tcttggatta ctctatctgc tataaaattc aatgttgaat
      481 ttgaagaaca aaacgcaacg ccgattccac aatcctttat catgcaaata tcattttcat
      541 cgtcacccac agcaattata ttgtgtaaat caatatgata gcggtgtgca acttctcgca
      601 tggcgttgga tttacaaaaa tcatgattac atgtactatc tttagtacgt aaaaacgcag
      661 aaggaatttt gacttcccct gttgccatgc cgtttgaaaa ttctaattcg tttgcgatag
      721 aaaagtccaa atcaaattta tttacaagat gatttgttat gcaatcgtag ctatcactta
      781 ttataccgca tatataacct ttttgtttaa actccttaac aattctttca aaatcattaa
      841 cagtaggaat gccatctaat gtttccagca attcgtttat ttttaaacct ttaagtaatt
      901 gtgcaattaa ttttgttcgg acataagggc tactattttc agctacaatt tcaattaatt
      961 tttctttaaa tttataattt gtagccagtg ttaaaatgaa actttttgta agaatggtgt
     1021 tatccatgtc gaaaattaac attttgttta aaccaagcag actttcctta atagcaaaat
     1081 ccatttggtc tctaatcata gcaaaattac caagtgtctc caaattagag tgttttatta
     1141 gttctgcacg ttttaagatt gcccttgaaa cttctttaga cattttagca agttgttccc
     1201 acgactgcat cctattttct atatatccta tatttacttc tacaattcgt gctttttcct
     1261 ggaacatatc aataagcagc ccaacgtcga caccataatc gttttcgaaa gtagcctttt
     1321 ccagaatact tttttttgct gcaatcattc cacttaaggg ttgagaaaaa tgagcaagtt
     1381 cagggaaaag aaggcttaat aaaggttttg ccactaattc agtaaccctg ccggcttgcc
     1441 tattaaatgt tgcttttaca aaatctgcag tattgtttat tattgggtcg gttagtaaat
     1501 taacaatatt ttcaggatag gtagttatgt cagcgtctaa atatacaatt ataccatttg
     1561 tggcgagtag cattccctca tacattgaag aacccttacc caattttgtg cttgtataaa
     1621 tctttacgtt ttttgccctg gcaattttta ctgtgctatc caaagattta tcatcaacta
     1681 ccaaaatttc tgatacgttt gcagattttt ccacaagttc aattacctgc gagattgtat
     1741 tttgttcgtt gagtgtgggt ataattactg ttatcatttt gtaaagggtt aaatgagcgg
     1801 aatcggattt tgtaaaaatg cttattgtgg ttgcaagcaa cagaatgcta gttaaaacgg
     1861 cagggaaaac gtcgtgatac tagccaaaat acaaatataa taaatttatt cgaggtatta
     1921 atttctccat ttgaccgagg aggagcgcct caactgaata catcgcttag ggcgcaaccc
     1981 ggccgatgtt gctttcagtt aaaaatgcag gcagggcatc ttcggataga taggcaagcc
     2041 cggaattgcc tagttaccag ctttacatat ttcattttta atgataatcc ccctataact
     2101 ccggcgcaac agggaatagc aataggttta ggtatggaag tttagtatac caacacaaca
     2161 tgccccatca aaaatttgcc ccataggagg tctcgcgtga aagataaacc acgaagcata
     2221 acccgagtgc aaccgccctg gctaggctat atttttctac acatggatgt cagcctatat
     2281 tctacgggtt ttgaagtact gatcgtaagt ggcagatgat tcgccaaatt tctcatatac
     2341 agtctccgaa atcttgtagg tgcagtgttc ccccacaatt cccgagaaga aaatctcatg
     2401 ccgcttggat ttgtcgtcaa tcatgataag ccttaccaga cgagggtgta catctgaagc
     2461 agccagcttg gcatggcaaa tgggacagta gtagtccagc atttcgccat tttcaatttt
     2521 aacatgaggg ctactcacct tggcatagtt tccaagttca ggactaagca ggataagacc
     2581 tcgtaatccg ctttgcatgc taatagaaag aatcacgctg cctcctacaa ccaggtatcc
     2641 ctcacatttt ggacagataa aatcattatt cattttacat ggtgttggat tacagctata
     2701 agatacaaca aaaatttcct gaagtaaagc aaaagccaaa attattacaa aaaaatagga
     2761 gaaggaccag ccttaaatga ccatgatttt gtggttcagc aggttggcgg ctaaaatgtg
     2821 tgttggtatg tttatgcgtc ttgtgggttc ataagctgaa catttagcta acggattact
     2881 atttcgtcaa taaatagcca ggatgattcg ccggcacttt catgcgacga tggcagtttt
     2941 ccatacgatt ttgcaaatac ctttacgtag cgagcctcga cattaacagc tactccgagt
     3001 tcttttacta tgggcttttc taaagtatcg gggatgtcgt tgttgaccac tccaatgttg
     3061 gagtagctag taccatcgga cgaaacagag aaggtaactt gccggggcat cataatccat
     3121 ggcgaggcat cctgaaggaa ctctgctcca agatagtgaa gttcacgttc atggcccagg
     3181 tcaacaattg cctcaaaatc ctgattttgg tagccctgcc agcctcccaa cctgaaattt
     3241 gtagagcctc tttgcccgtc aaccagcgca agtttcccac caccatcgta aagagggcta
     3301 gggatgcttt tcagctcaac atttttaatt ccctcgattc ggataaagga accctcaact
     3361 atggcgctgg attcgccacc cggaagaacg gagtaggctt taacccgttt ggagctctta
     3421 agattcagcg tattcccttg tgtccatatt ggaatggctt cggggtggtt tatttctgcc
     3481 accgaataaa aagttgaggc ttggggctgg acagttgtga gggttagctg caagctgtcc
     3541 atgtatgttt tagttgaggt gttgaaggcc ggagcagtag ctataaaatg ctcatcaatt
     3601 gatgaggttg gcatatcagc atcggtgctg ccccagccgc tattaggagt aggcgacata
     3661 gctaaacgga gttcgccccc tgccattatc tcgttgtagt caatccagca gcgggtaatg
     3721 ggtttaccat taagcgaagc agagttgatg taaatatttt caggcgagtt gttctcggct
     3781 accaccgaaa aatactttcc gttttccagg ttaaaggtaa ttttactgaa gagcggagaa
     3841 ccaatgacga gttgtgtact tcccgggcag accggataaa atcctgctgc acttaaaatg
     3901 taccatgcgc tcatttgtcc gcaatcctcg ttaccacaaa ggccatcggg ttttgaggtg
     3961 tacatctcgt ccataatctt gcgtacaagc ttttgtgttt tccacggttg cccggcatag
     4021 cagtaaaggt atgccatatg gtggctgggt tcgtttccat gagcatactg cccaataagg
     4081 ccggttatat caacttgatt tttccccatc agctgggatg aggttgaaaa tagctcatcc
     4141 aacctaccga tgaaattttt ctttccgccg tggagttcaa taaggccgga aacatcatga
     4201 ggtacataga agctgtactg ccagctgttt gcctcggtga agttttggtc aacctcggat
     4261 gggttaaagt tatctttcca accgccgttt atccgtggtc gcatgaagtg agtggatggg
     4321 tcgaagagat tcttgtatga ctgtgcccgc ataatgaact tttggtaggt agagctatcg
     4381 gcaatggatt tcgcaaattg agcaatacac cagtcgtcat aggcatactc cagtgttttg
     4441 gaaaccgact catgctccat atcgccagga ataaaaccgt actgcctgta ctcctttagc
     4501 ccaaaatggt tgagatttgc gctatggagc attgccttca gggctagatc gggatcaaaa
     4561 tttctgattc cttttatcca tgcgtcggct ataacgggaa ccgaatggta cccaatcatg
     4621 cactcggttt cgttggcagc cagcgcccac acgggcaagc gaccaccgaa gcggtaatca
     4681 gacaggaaag tgttaatata gtcggtagta cgtcgagtat caataattgt
//
LOCUS       DAOOQX010000062         4729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_21575_length_4729_cov_3.697689, whole genome shotgun sequence.
ACCESSION   DAOOQX010000062 DAOOQX010000000
VERSION     DAOOQX010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21575_length_4729_cov_3.697689"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLX53_01700"
     CDS             <1..325
                     /locus_tag="PLX53_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOY71825.1"
                     /translation="KDPLLIYKLEAYELFRTMLENLNRDIVSILAKAFIPLRSANEVQ
                     EARQRSHTDMSRYQTSRTDNLQAGAPTQQPNREPVKVGIKVGRNDPCPCGSGKKYKNC
                     HGRDE"
     gene            464..2260
                     /gene="argS"
                     /locus_tag="PLX53_01705"
     CDS             464..2260
                     /gene="argS"
                     /locus_tag="PLX53_01705"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOY71826.1"
                     /translation="MSTEQYIQDIVAETVLKLYGVKPEVNQLQIQKTRKEFEGDYTLV
                     AFPLLKLTRKSPEETCRQIGEMLLQSYPEVSAINVIKGFLNITLSKTYWSGVINEIAS
                     TEDYGFAPTPTENANPTIVEFSSPNTNKPLHLGHIRNNLLGFSISRILEKSGKRVIKV
                     NLVNDRGIHICKSMVAWLKYGNGETPQTSGKKGDHLVGDYYVRFEKEYKLQVEQLTSS
                     GMDKDEALTKAPIIIEAQEMLRKWEAKDAETIRLWKMMNGWVYEGFEKTYQNLGITFD
                     KIYYESQTYLVGKKVVNEGLDRGIFFRKSDGSVWVDLTADGLDQKLLLRSDGTSVYIT
                     QDIGTAIERYNEFHFNEHIYVVGNEQDYHFQVLKLVMKKLGYTWYEALYHLSYGMVLL
                     PEGKMKSREGTVVDADDLISEMLQTAGDVSRELGKLDQMSDEEASETIRMVGLGALKY
                     FILKVDPKKNMTFNPGESIDFNGNTGPFIQYTHARIRSVLRKAGNVNDLLNQKVTPES
                     ISFQKELELIQLLHLFPDTIKIAAQTHNPAEIANYTYELAKQYNQFYQDLPILREPNV
                     EVKHFRLLLSQAVARVIKQAMWLLGIDVPEVM"
     gene            2379..3713
                     /gene="ffh"
                     /locus_tag="PLX53_01710"
     CDS             2379..3713
                     /gene="ffh"
                     /locus_tag="PLX53_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOY71827.1"
                     /translation="MFENLTEKLERSFKILKGEGKITEINVAETLKEVRKALLDADVN
                     YKVAKSFTDEVKRKALGMNVLNAIRPGQLMVKVVHDELAELMGGKAANINLNGNPAII
                     LIAGLQGSGKTTFSAKLASYLKTKKGKSPLLVAGDVYRPAAIEQLKVLGDQVGVPIFT
                     VPGSSNPVDIAKLSIKQAKTLGRDTIIIDTAGRLAVDEEMMNEVAAIKKVVSPQEILF
                     VVDSMTGQDAVNTAKEFNDRLNFDGVVLTKLDGDTRGGAALSIRAVVDKPIKFISTGE
                     KLDALDVFHPERMADRILGMGDIVSLVEKAQEQFDADKARELNKKILKNRFNFNDFLE
                     QIGQIKKMGNLKDLAGMIPGVGKAIKDVDLNDDAFKSTEAIIKSMTPAEREEPSLING
                     SRRKRIADGSGTSIVEVNRLIKQFEDMRKMMKMASGGGAKNLARMMSQQPRR"
     gene            3849..>4729
                     /gene="folD"
                     /locus_tag="PLX53_01715"
     CDS             3849..>4729
                     /gene="folD"
                     /locus_tag="PLX53_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOY71828.1"
                     /translation="MELLDGKKISEEIRAEIASEVEKIKSQGGKIPYLVAMLVGHDGA
                     SETYVHNKELACAQVGFRSQVLRFEETISESGLLAEIQKLNANPEVDGFIVQMPLPKH
                     IDEQKIIEAIDPKKDVDGFHPVNVGRMVIGLPSYISATPDGIVELLRRYKVETAGKHC
                     VVLGRSNIVGRPVANLLLHKGYPGDCTVTICHSRTPNLKELTLQADILIVAMGKPEFV
                     TADMVKDGAVVVDVGITRVPSQKTKSGWKLVGDVKFDEVAPKCSFITPVPGGVGPLTI
                     ASLLKNTLKAARKEVYG"
BASE COUNT         1461 a         1048 c         1155 g         1065 t
ORIGIN      
        1 gaaggatccc ctgttgatat ataaacttga agcctacgag ctgttccgca ccatgctcga
       61 aaacctgaac cgcgacattg tgtccatact tgccaaggcc tttatcccgt tacggagtgc
      121 aaacgaggtt caggaggcaa gacaacgtag ccacaccgac atgagtaggt atcaaacctc
      181 acgaacggac aaccttcagg caggtgcacc aacacaacag cccaacaggg aacctgttaa
      241 ggtaggcatc aaggttggta gaaacgaccc ctgcccatgc ggtagcggga aaaaatataa
      301 aaactgccac ggaagagacg agtagaattt tacgctcgcc tgtcgggcta aagctaccta
      361 tcaacatcgg gtataattct ggctgtggag cagtggcttt tgcaaacaca gccattttat
      421 tacttcagca tcaggaagca tggatttcaa acaaaaccaa ataatgtcaa ccgagcagta
      481 cattcaggat attgtagcgg aaaccgttct aaaactttac ggagtaaaac cggaagtaaa
      541 ccagctccaa attcaaaaaa cacgtaagga gtttgaaggc gactacactc tggtcgcctt
      601 tccactgttg aagcttacgc gaaaatcgcc cgaagaaacc tgccgccaga ttggggaaat
      661 gctacttcaa tcgtatccag aggtttcagc aattaacgta ataaaggggt ttctgaatat
      721 caccctatct aaaacgtact ggtcaggcgt aatcaacgag attgcctcaa ccgaagacta
      781 tggatttgcc cctaccccaa cagaaaatgc taaccctacc attgtagaat tttcgtcgcc
      841 caacaccaat aagccccttc acctgggaca tatccgcaac aaccttctgg ggttttccat
      901 ttcgaggata ctggagaaaa gcgggaaaag ggttattaag gtaaacctgg tgaacgatag
      961 gggaatccac atttgcaaat cgatggtagc ttggcttaag tacggaaatg gcgaaacacc
     1021 ccaaacgtca ggtaaaaagg gcgatcacct ggtaggtgac tactacgtca ggtttgaaaa
     1081 ggagtataag ctgcaagtag agcagctcac gtcaagcggc atggacaaag acgaggcatt
     1141 aaccaaggca cctataatca tagaagctca ggaaatgctc agaaaatggg aagctaaaga
     1201 tgccgaaacc atcaggcttt ggaaaatgat gaatggttgg gtttatgaag gtttcgaaaa
     1261 aacctaccaa aatctaggaa taaccttcga caaaatatac tacgaatcgc aaacctacct
     1321 ggttgggaaa aaggtagtga acgaggggtt ggaccgggga attttcttta ggaagagtga
     1381 tggttcggtt tgggtcgatc tcaccgccga cggcctcgac caaaagctac tgcttcgctc
     1441 cgacggaact tcagtttaca tcacccagga tattggcaca gcgatagagc gctacaacga
     1501 gttccatttt aacgagcata tttacgtggt aggcaatgag caggactacc attttcaggt
     1561 gcttaagctg gttatgaaaa aactcggcta cacatggtac gaggctctat accacctctc
     1621 ctacggaatg gtgctgctgc ccgaaggaaa gatgaaatcg cgcgagggaa ccgtggttga
     1681 tgccgacgac ctcatatccg aaatgcttca aactgccggt gatgtatcgc gagaacttgg
     1741 caagctagac cagatgagcg atgaggaggc ttcagaaaca atcagaatgg ttggcctggg
     1801 agcacttaag tacttcatcc tgaaggttga ccccaaaaaa aatatgacct tcaacccggg
     1861 agagtctatt gacttcaacg gcaacaccgg cccattcatc cagtacaccc atgcacgcat
     1921 acgctccgtt cttcgtaagg cgggcaatgt gaatgattta ctgaaccaaa aagtaacacc
     1981 cgaaagcatt tcatttcaaa aggaactgga actcattcaa ctcctacatc ttttccccga
     2041 tacaattaaa atagctgctc aaacgcataa cccagcagaa attgccaact atacctacga
     2101 gctggcaaag cagtacaacc agttttacca agacctgccc atactgcgag aacccaacgt
     2161 cgaggtaaaa cactttaggc ttctgctttc gcaagcagta gcccgtgtta ttaaacaagc
     2221 aatgtggctt ctgggaattg atgtgccgga agttatgtag atataacaga agctttatct
     2281 aaacatagcc ttttagctgc acttccaaaa tggctttatg ttgaaaaagc catcattttt
     2341 gataactttg cagagaataa taaatagtaa aacaatttat gtttgaaaat ttaaccgaaa
     2401 agctcgaacg atcctttaaa atcctcaagg gagaggggaa aataaccgaa ataaacgtag
     2461 ccgaaaccct taaggaagtt cgtaaagctc ttctcgatgc cgatgtcaac tataaagttg
     2521 cgaagagttt taccgatgag gtaaagcgaa aagccttggg aatgaatgtg cttaatgcca
     2581 tcaggcctgg acagctaatg gtaaaagtag ttcatgacga gctagctgag ctaatgggtg
     2641 gtaaggcagc caatatcaac cttaacggaa acccagcgat tattctgatt gccggccttc
     2701 aaggctccgg taaaacaact ttctctgcca agctggcaag ctacctcaaa accaaaaagg
     2761 gaaaatcgcc gttgctggtt gccggtgacg tttacagacc ggcagctatt gagcagctta
     2821 aggtgcttgg cgaccaggtt ggcgttccaa ttttcaccgt tccaggtagc agtaatcccg
     2881 ttgacatagc taaactctct attaaacagg ccaaaacgct cggaagagat acgattataa
     2941 ttgatactgc cggtcgtctg gctgttgatg aggagatgat gaacgaggtg gccgccatca
     3001 aaaaggtcgt ttcgcctcag gaaattctat tcgtagttga ctccatgact ggtcaggatg
     3061 cggttaacac agcaaaagaa ttcaacgaca ggcttaactt tgacggcgtg gtgctcacca
     3121 agctcgatgg cgatacccgc ggtggtgcag ctctatccat aagggccgtt gttgacaagc
     3181 ccattaagtt tatcagcaca ggcgagaagc tcgatgccct cgatgttttt catcccgaac
     3241 gtatggccga ccgcatactg ggaatgggcg atattgtttc gctggttgaa aaagcgcagg
     3301 aacagtttga tgcggataaa gcccgggaac ttaacaagaa gatacttaaa aacaggttca
     3361 actttaacga ttttcttgaa cagataggcc agataaagaa aatgggaaac ctgaaggacc
     3421 ttgccggaat gatacccgga gtgggtaagg ccataaaaga tgttgatcta aacgatgatg
     3481 cctttaaaag caccgaggcc attattaaat ccatgacccc ggctgaacgt gaagaaccgt
     3541 cgttgattaa tggtagccgc cgcaagcgaa ttgccgatgg cagcggaacc tccattgtgg
     3601 aggtaaaccg gttgattaag cagtttgagg atatgcgtaa aatgatgaag atggcatccg
     3661 gaggtggtgc gaaaaatctt gcacggatga tgtcacaaca gccaagaagg tagagaaaag
     3721 tagagtgaga tattaaataa tacaaggata aaagcgaaat cgtaaaccct gaaaagttaa
     3781 ggggaggaag atggatgcct taaaccttaa accttaaacc ttgaatcttg aatcttgaat
     3841 cttgaataat ggaactacta gatgggaaaa aaatatctga agaaattcga gccgaaatcg
     3901 cctcggaggt tgaaaagata aaaagccaag ggggaaaaat cccctacctg gtagccatgc
     3961 tggttggcca tgatggggca agcgagacct acgttcacaa caaggagctg gcctgcgccc
     4021 aggtaggatt caggtcgcag gtgctacgct ttgaggagac aattagtgag agcgggctac
     4081 tggctgaaat acaaaagctg aatgccaacc ccgaggtgga tggcttcata gtgcagatgc
     4141 cacttcccaa gcatattgat gagcaaaaaa ttattgaggc cattgacccc aaaaaggatg
     4201 ttgatggctt tcacccggta aacgtgggtc ggatggttat tgggcttcct tcctacattt
     4261 cggcaacacc cgatggaatt gtggagctac tccgaaggta taaggttgaa actgccggaa
     4321 aacactgtgt ggtgctgggt aggagcaaca ttgtaggtcg ccctgtagcc aacctgctgc
     4381 ttcacaaggg ctaccctggc gattgcacgg ttactatctg ccacagccga acacccaact
     4441 taaaagaact taccctgcag gccgatatac taattgtagc catgggaaag cctgaatttg
     4501 ttacagccga catggtaaag gatggggcgg tggtggttga cgtggggata accagagttc
     4561 ccagccaaaa aaccaaatcg ggatggaagc tggtgggcga tgttaaattt gatgaggtgg
     4621 cgcctaaatg cagctttatt accccggtac ccggtggtgt tggtcccctc accattgcct
     4681 cgttgctcaa aaataccctc aaggcagcca ggaaggaggt ttacggata
//
LOCUS       DAOOQX010000063         4685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_21821_length_4685_cov_3.898488, whole genome shotgun sequence.
ACCESSION   DAOOQX010000063 DAOOQX010000000
VERSION     DAOOQX010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21821_length_4685_cov_3.898488"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLX53_01720"
     CDS             <1..333
                     /locus_tag="PLX53_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOY71829.1"
                     /translation="FEEYNEVPYEKYFEIDKVKDTITMNKFLSEKPIFRKHDLVTDGN
                     IFFAKFDLILCRNVIIYFNYSLQNKVFELFHSNLYPKGVLLLGMHETILGPWATKFEK
                     LGQSYVKK"
     gene            509..2866
                     /locus_tag="PLX53_01725"
     CDS             509..2866
                     /locus_tag="PLX53_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOY71830.1"
                     /translation="MKISQRIQLIIVPLMILLFSASGVTLNYLSKNRVRESIRIQEGV
                     YIDKLIQIVDMVEAKTGSGLSSDDIFELRTYFLSPAYYSTDYPAIISTNGVYLLHTTL
                     QGQRLPSAATAQIRKSKSTDGYIYYDDYSGNKPEKRLLCYKFYKPYNAYIAISVSMNE
                     ALAPMKSNRMAIIVFIILASLLTAFAIPLLLKPIIRIINLVNIKLRQLAKGDIPNPIK
                     ITSKDELGEMVTSLNQLMEGLKSTTSFAHQIGQNQLEHTFQPLSSKDELGNALLDIQS
                     NLKHALEEEQRRKEEDYIRGWVNEGLAKFGDILRQNNNNLTLLADSIIQNLVNYLEAN
                     QGGIFIYNDSENDKHLEMLSAFAYNRKKFISKTIPLGEGLVGACALEKKTIYLKEIPA
                     DYIAITSGLGEATPTALLIVPLKLEDEIFGVVEVASFREFKPYEIEFVEKIGESIAST
                     LFAVKNSIRTNMLLEQSQQQREEMAAQEEEMRQNMEEMQATQEEMLRKNIELELITNT
                     INQSLLSLTLSEEGYIADSNQNFVETLGYSKTELEGKMLADIVNPEQRTDFQISWKKV
                     RAGQTITETIHVLNRNGNSHYLMVTVSPELDPAGAITKVLLIGQDITEMKQLEIKAQR
                     QAEEIEQNLKQLEKEQELSAQRQKDMSTLLEALDQNCLVSIIEPDGLITYINNKNVEV
                     LGDKKEDIEGKFLHDIDYSAKHRPAEYKKFWDNILNGKKQQREFSLTVKGEVKWVLEN
                     FTPVMDESGKLYKIINIGFDITENKNKEAELTKIIEQMKNKPKNL"
     gene            3013..3846
                     /locus_tag="PLX53_01730"
     CDS             3013..3846
                     /locus_tag="PLX53_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOY71831.1"
                     /translation="MMQYEIGIVETRNVIKTLLDDYGYDFRDYALTSFKRRLEQIISN
                     NGLKDADGLIGKLKGNPTFFDQFLNEVNIDTTEMFRDPSFWRTLRDDILPEIIRNSTT
                     KPRIWIASFDSGEELYSLTIILKEMGALDKVQIYSNVFTNATIQKITSGQLDPKDIEI
                     NEANYVRLNGKSQYSSYYKVEGNKYFFDSTLVKDVNFIKQTPIIDKDPGAVKLVLFRN
                     QALYYNQLLQERVLQKIAESLVAGGFLALGIKETLENTNANNKFTVFNETERIYKRKN
                     V"
     gene            3852..4412
                     /locus_tag="PLX53_01735"
     CDS             3852..4412
                     /locus_tag="PLX53_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOY71832.1"
                     /translation="MYKAVIIGGSAGSFQVITRILHSLPASFPLPVLLCLHRLKHVRS
                     GFVEALSLKSGIPIIEPDDKEQIKPGKAYLAPANYHMFIELGNKIALSTEDPVNHSRP
                     SIDLSFLTAAQVYRDKLIGVILSGANRDGAYGLKKIKDLGGLAIVQDPNECQVRTMTE
                     ASLKMTPVDHVYNTSEIIRFLQTIKT"
     gene            4417..>4685
                     /locus_tag="PLX53_01740"
     CDS             4417..>4685
                     /locus_tag="PLX53_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71833.1"
                     /translation="MKAFSSKSKLLPLLLLVLLIAGVVITFGLIHNTLQNTGGSLPAI
                     GYVLLFLPAIISIGLYAMLNNLQVKSAKLEAEIAELKAAFALLQQV"
BASE COUNT         1493 a         1118 c          954 g         1120 t
ORIGIN      
        1 tttgaagagt acaacgaggt tccctatgaa aagtactttg aaattgacaa ggtaaaggat
       61 accataacca tgaacaaatt cctgtcggaa aaaccaattt tccgtaagca cgacctggta
      121 accgacggga atattttctt tgccaagttc gacctcatcc tttgcagaaa tgtgattatc
      181 tacttcaact actcccttca gaacaaggta tttgaactat ttcactcaaa cctttacccc
      241 aagggagtgc tgctgcttgg aatgcacgag accatacttg gcccctgggc cactaagttt
      301 gaaaaacttg gacaaagcta tgtgaaaaaa tagttatgaa gcaacctttt gataccgaca
      361 atttcaacac cgctgattgc acaattggca acgtactatg tagaatactt ttttttggtc
      421 agaggcaagc tggtggaaaa cttatcatca cgttaagtaa gcttttaggg aaaaatgtaa
      481 cgaattgtaa ctttaaattc tacatgaaat gaaaatttca caacgtattc agctcattat
      541 cgttccactg atgatcctgt tgttttccgc ttccggagta acgctgaact acctgtcaaa
      601 aaatagggtt agggaaagca tacgaattca ggaaggagtg tacatagaca agctgataca
      661 aattgtcgat atggtggaag caaaaacagg aagcggccta tcctccgatg atattttcga
      721 gctcaggact tacttcctct cgccagccta ctatagtacc gattaccccg ccattatctc
      781 aaccaatggc gtttacctgc ttcacacaac acttcaaggg caaagacttc cctctgctgc
      841 aactgcccag attagaaaga gcaaaagcac cgacggctac atatattatg acgattattc
      901 aggaaacaaa cccgagaagc ggttgctatg ctacaagttc tataagccct acaatgccta
      961 cattgccatc tcggtcagta tgaacgaagc cttggctcca atgaaatcca accgcatggc
     1021 aattatagta tttatcattc tggcgtccct tctaacagcc tttgcaattc ctttactcct
     1081 taaacccatc atacgcatta taaacctggt gaacataaag ctccgccagc tggcaaaagg
     1141 cgacattcca aaccccatca aaataacatc aaaggatgag cttggtgaaa tggttacctc
     1201 gctaaaccag cttatggaag ggctaaagtc aacaaccagc tttgcacatc aaattggaca
     1261 gaaccagctg gagcacacct tccaacctct gagcagcaag gatgaacttg gcaacgccct
     1321 gcttgatatc caatcaaacc tgaagcatgc cctggaggaa gaacagcgca gaaaggagga
     1381 agactacatc cggggctggg taaacgaagg tcttgcaaag tttggagata tcctgcgtca
     1441 aaacaacaac aacctaaccc tattggccga cagcatcatt caaaatctgg tcaactacct
     1501 cgaggcaaac caggggggca ttttcattta caacgattca gaaaacgaca agcacctcga
     1561 gatgctttct gcatttgcct ataaccggaa aaaattcatc agtaaaacca tcccccttgg
     1621 cgaaggactt gttggtgcct gcgctcttga gaaaaagacc atctacctca aggagatacc
     1681 tgctgactac attgccataa cttccggcct aggagaggcc acccctaccg cactgctaat
     1741 cgttccactt aaacttgagg atgaaatttt tggagtagtt gaggtggcct cattcaggga
     1801 atttaaacct tacgaaattg agtttgttga aaaaataggc gagagcatcg cttcaaccct
     1861 ctttgccgtt aaaaacagca tacgaaccaa catgcttctc gagcaatcgc aacagcaaag
     1921 ggaggagatg gccgcacaag aggaagagat gcgccaaaac atggaggaaa tgcaggccac
     1981 ccaggaggaa atgctacgga aaaacattga gttggaactc attaccaata ccattaacca
     2041 gtcgctactt tctcttaccc tttccgaaga aggctacata gcagactcca accaaaattt
     2101 tgtggaaacc cttgggtact ccaaaaccga gcttgaagga aaaatgcttg ccgacatcgt
     2161 taaccccgaa caacgcaccg attttcaaat atcatggaaa aaagttaggg ctggacaaac
     2221 cattacagaa actattcatg tgctcaaccg aaacggcaac agccactacc ttatggttac
     2281 cgtatcgccc gagttggacc ccgctggagc tataaccaaa gttctgctta ttggtcagga
     2341 catcaccgaa atgaagcagc tggagattaa agcccaacgc caagccgaag agattgaaca
     2401 aaacctgaaa cagctggaga aggaacagga gctatcggcc cagcgacaga aggatatgag
     2461 caccctgctg gaggcgctag accaaaactg cctggtttca attatcgaac ccgacggatt
     2521 gataacctac atcaacaata aaaacgtcga ggtgctgggc gataagaagg aagatatcga
     2581 gggaaaattc ctccatgaca ttgactactc ggccaagcat cgaccggctg agtataaaaa
     2641 attctgggac aacatcctga atgggaaaaa gcagcagcgg gagttttccc ttaccgtcaa
     2701 gggggaagtc aaatgggtgc ttgaaaactt tacccccgta atggacgaaa gtgggaaact
     2761 ttacaagata atcaacatcg ggttcgatat aaccgaaaac aagaataagg aagcagagct
     2821 gacaaaaata attgaacaga tgaagaacaa acccaaaaac ctatgacata acacattcat
     2881 gattaatcga gttatccatt cgcctctgga tttcaacatt ttttaattca cttttaattt
     2941 ggcacactct aaatgatgaa caagctaaac tggaatagag ttaatttttc acttttgaaa
     3001 tgacgcttga caatgatgca gtatgaaatt ggcatagtag aaacccgtaa cgttattaaa
     3061 acgcttctgg atgactatgg ctacgacttt agagattacg ctctaacctc gtttaaacga
     3121 aggcttgagc agataatctc aaacaacggc ctaaaggatg ccgacgggct aattggcaag
     3181 ctaaagggca acccaacctt ttttgaccag ttcctaaatg aggtcaatat cgacaccacc
     3241 gaaatgtttc gcgatccttc cttttggcga accctacgcg acgatattct acccgaaatc
     3301 atccgcaact ccaccaccaa accccgaatt tggattgcct cgtttgattc cggcgaggag
     3361 ctttactcat tgaccattat cctgaaggaa atgggagcac tcgataaggt tcagatttac
     3421 agcaacgttt tcaccaatgc caccatccag aaaattacca gcggccagct tgaccctaag
     3481 gatattgaaa taaacgaagc taactacgtt aggctgaacg gtaaaagtca gtactcctcc
     3541 tactacaaag tagaggggaa caagtacttt ttcgatagca cactggttaa agatgtgaac
     3601 tttattaagc aaacccccat aatagacaag gatcccggag cggtaaaact tgtccttttc
     3661 cgcaatcaag ccttatacta caaccagctg ctgcaagaac gggttttaca aaaaattgca
     3721 gaaagtttgg tggctggtgg gttccttgcc ttgggaataa aagaaacttt ggaaaataca
     3781 aacgcaaaca ataaatttac cgtatttaat gaaaccgaaa gaatttacaa gcgcaaaaat
     3841 gtttaatgac tatgtataag gcagtaatta taggcggttc agcaggtagt tttcaggtaa
     3901 ttacccgaat actgcattcc ctacctgcat cttttcccct tcccgtcctt ctgtgcctac
     3961 ataggctgaa acacgtccgc agcggctttg ttgaagcgct ctcgctaaag tctggtattc
     4021 ccataattga gcccgatgat aaggaacaga taaagcccgg caaggcatac ctggccccgg
     4081 caaactacca catgttcatt gaacttggta ataaaatagc cctttccact gaggaccctg
     4141 ttaaccactc gcgcccaagc atcgaccttt catttttaac tgccgcccag gtgtaccgcg
     4201 ataaactcat tggtgttatc ctatcggggg ctaaccggga tggtgcttac gggctgaaaa
     4261 aaataaaaga tctgggcggt ctggcaatag ttcaggaccc caacgagtgc caggttagaa
     4321 ccatgacaga ggcctcccta aaaatgacac ccgttgacca tgtgtacaac accagcgaaa
     4381 taatcagatt tttgcaaacc attaaaactt gaagaaatga aggcctttag ctcaaaatca
     4441 aaactccttc cattgctcct gctggttttg cttattgccg gtgtagttat cacctttgga
     4501 ttaatccata acacactcca aaacacagga ggcagccttc ccgctatagg ttacgttctt
     4561 ctattcctcc ccgccattat tagcatcggt ctgtacgcca tgctcaacaa ccttcaggtt
     4621 aaatccgcaa aactcgaagc agaaatagcg gagctaaagg cagcctttgc gctccttcaa
     4681 caggt
//
LOCUS       DAOOQX010000064         4623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_22192_length_4623_cov_3.229422, whole genome shotgun sequence.
ACCESSION   DAOOQX010000064 DAOOQX010000000
VERSION     DAOOQX010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22192_length_4623_cov_3.229422"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="PLX53_01745"
     CDS             complement(<1..1240)
                     /locus_tag="PLX53_01745"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOY71834.1"
                     /translation="MKSDIEIAHEVKMKPVTEIAGKLGIDADDLVLYGKYKAKVPLKY
                     IDKNKYQKNKLVLVSAISPTPAGEGKTTVSIGLAQALNRLGKQTTVVLREPSLGPVFG
                     VKGGATGGGYSQVLPMEDINLHFTGDFAAIERAHNLLAALIDNNIQSKKRSLGIDPRT
                     VRWKRIMDMNDRSLRHIIVGLGGTSHGVPRETGFDITAASEVMAILCLAENIDDLKKR
                     LGNIFIGFTYDKKPIYARDLNAQGAMAALLRDAIMPNLVQTIEGTPAIIHGGPFANIA
                     QGTNSVLATKLGLSLSDFVVTEAGFGFDLGAEKFIDIKCEYAGLNPNTVVLVATVRAL
                     KYHGNKPLKEVSQPDLEALKRGIGNLQKHVENMKQFGICPVVAINRFTSDTDEEVEYI
                     KQVCRDMSVQVAAVDVWGKGG"
     gene            complement(1529..2539)
                     /locus_tag="PLX53_01750"
     CDS             complement(1529..2539)
                     /locus_tag="PLX53_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71835.1"
                     /translation="MNRVILFSLTALVLLISCKPNRTERIDPTNVPASIQLERFEQDL
                     FSASPDSLQTLVPELRKKYGRFVDLFSEGIIHIGKTDSPEYCNYLSQFLSDTMVQNAY
                     RLSQQKFQNISSIKVCLNEAFNRFHCYFPDKPVPKIYTFDSGYNLSLAVDDSILAIGL
                     DRYLGSNTVQYKLLGIPRYQVRNMRPEKIPSDAIRAWMYGSFSFHDSTDNLLSNLIYE
                     GQVMYLTKQMLPQESDSLIFGFTPSQHRWCLKNEKAMWTNLIENKLLFSTNTLDINKF
                     INDAPFTSGFPQESPGRAAVWIGYRIVESLMKNNDTLTLSEMMGISDYQKFLAMSRYK
                     PR"
     gene            2797..4275
                     /gene="dnaB"
                     /locus_tag="PLX53_01755"
     CDS             2797..4275
                     /gene="dnaB"
                     /locus_tag="PLX53_01755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOY71836.1"
                     /translation="MGKVPPQAIELEEAVLGAIMVEKDAVVIVSEILKPECFYKETHQ
                     EIFKAIQDLSSRLEPIDLFTVTEELRRQNKLEEIGGAAFLAQLTTKVGSAAHLEYHAK
                     IIAQKFIQRELIRVSTDIQNRSFDEDVDVDELLDYSEMELFKVAEGNIKSDTQPITTI
                     IKKAIKQIEEAGKREDGLSGVPSGFTALDRLTSGWQNSDLIIVAARPSMGKTAFVLSM
                     ARNMAVDHDVPVAFFSLEMSNLQLVNRLIVSESGIASEKIRNGRIDQSEWTQLTVKIK
                     NLSEAKIFIDDTPALSLFEFRAKCRRLKAQYDVGVIIIDYLQLMTGPSETKGNREQEV
                     STISRSLKAIAKELNVPIIALSQLNRSVETRGGYKRPQLSDLRESGAIEQDADVVIFI
                     HRPEYYGFMEDEDGNSTQGIAEIIVAKHRNGPVDDIKLNFRKEMAKFSDFDDVHYGDI
                     QPSHSPFTSSVPVTFSSKMNEDTHGDYDFLSGQGVDSSDIPF"
     gene            complement(4396..>4623)
                     /locus_tag="PLX53_01760"
     CDS             complement(4396..>4623)
                     /locus_tag="PLX53_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOY71837.1"
                     /translation="LSGYHKPDPRAFGYALSSANIRKNQGIMVGDDLEVDIVGARKFG
                     MDQIFFNPERKPHGEKVTYEVETLKEITQIL"
BASE COUNT         1166 a         1042 c         1126 g         1289 t
ORIGIN      
        1 ctccaccttt tccccaaaca tcaacagcgg ctacttgaac ggacatatca cggcacacct
       61 gtttgatata ctcaacctcc tcatcggtat cggaggtaaa gcggttgatg gctaccaccg
      121 ggcatatacc gaattgcttc atgttttcta catgtttctg caggttgcca attcctcttt
      181 tgagcgcctc caaatcgggc tgcgatactt ccttcaacgg tttattgcca tggtacttta
      241 acgctcttac agttgcaacc agaaccacgg tgttggggtt taagccggca tactcgcact
      301 tgatatcgat aaacttttcg gcgccaaggt caaacccaaa tccagcctcg gtaactacaa
      361 agtcgctgag cgataggccc agcttggtgg ctagaaccga gttggttccc tgggctatgt
      421 tggcgaatgg gcctccatgg ataatggccg gggtaccctc aatagtttga accaggtttg
      481 gcatgatggc atcgcgcagt agggcagcca ttgctccctg ggcgtttagg tcgcgggcgt
      541 agataggctt tttatcatat gtgaaaccaa taaagatgtt gcccagcctt tttttcaggt
      601 cgtcgatatt ctcggccagg caaagaatgg ccataacctc cgatgctgcg gtaatgtcaa
      661 atcccgtttc gcgagggacg ccatgtgatg ttcctcccag cccaacgatg atgtgtcgga
      721 gcgagcggtc gttcatgtcc ataatccttt tccaccttac ggttcggggg tctataccga
      781 gtgagcgttt tttgctttgg atgttgttgt caatcagggc tgccaacagg ttatgggcac
      841 gttcgatggc ggcaaaatcg ccggtgaagt gcaggttgat gtcctccata ggaagcacct
      901 gggagtatcc gccaccggtg gcaccaccct ttactccaaa taccgggcct aatgagggct
      961 cacgaagaac caccgtagtc tgctttccca accggttaag cgcttgcgcc aggcctattg
     1021 aaaccgttgt tttcccttca cctgctgggg tgggtgaaat ggccgaaacc agcaccagct
     1081 tgtttttctg gtacttgttt ttgtcgatat actttagcgg caccttagcc ttgtacttcc
     1141 cgtaaagcac caggtcgtcc gcatcaattc ccagtttgcc cgctatttcg gtaactggct
     1201 tcattttaac ctcatgggca atttcaatgt ctgatttcat tataatagat atttaaaata
     1261 gtttgtattt atactttgta ggttccaaag ggttgaatat gaaaatttaa aagacaggta
     1321 agttgaaaat taaatgatgt tgttgcgcta ttacttactg atattaatga ttagcaacaa
     1381 gctgtttcat tttttgttta tgctttgatg gttttttggt ccattttgtt ggtgccttca
     1441 gctttttaaa aatgcaattg gggttcatgc taatgctatt tctacttcgt tttgaaaatc
     1501 tttaaaagtg aaatttgcaa gtagcttact atctaggttt atacctcgac attgccagaa
     1561 acttttggta gtcggatatg cccatcattt cgcttagggt aagggtatcg ttatttttca
     1621 tcaacgattc cacaattctg tagcctatcc aaacggcagc cctgcctggc gactcctgtg
     1681 gaaatcccga ggtaaagggt gcatcattaa taaacttgtt aatatccagc gtgttggtgc
     1741 taaaaagaag cttattctca attaaattag tccacatcgc tttctcgttt ttcaggcacc
     1801 agcggtgctg gctgggtgtg aaaccaaaaa tcagcgaatc ggactcctgc ggtagcattt
     1861 gcttggtaag gtacatcacc tgtccctcat atattaggtt gcttagcagg ttgtcagtag
     1921 agtcgtggaa gctgaacgaa ccatacatcc aggcgcgaat ggcatccgaa ggtatctttt
     1981 ctggacgcat gtttctcacc tggtagcgag gaatgcccag cagcttatac tgaacggtat
     2041 tgctgcccag atatcggtca agcccaatag ccagaatgct gtcgtcaacg gccaacgaaa
     2101 ggttgtaccc cgagtcaaac gtgtaaattt ttggaactgg cttatcgggg aagtagcagt
     2161 gaaatctatt gaatgcttca ttaaggcaga ccttaatact gcttatgttt tggaactttt
     2221 gctgcgataa tcggtaggcg ttttgcacca tggtatcgct tagaaattgc gacaggtaat
     2281 tgcagtactc ggggctgtcg gtttttccaa tatggataat tccctcggag aataaatcga
     2341 caaaacgccc gtacttcttt cttaactcgg gtactagggt ctgcaggctg tcgggcgaag
     2401 cagaaaataa atcctgctca aacctctcca gctgaatgct agctggaaca ttggtcgggt
     2461 caatcctctc agtcctgttg ggtttgcagg aaatgaggag cacaagagca gtaagtgaaa
     2521 agagtatgac ccggttcatg caggttgaca tttgccccca aagatagcaa agcgttatgg
     2581 aacaagagta ttaaaattag ctaagcatca accaatattt tagtgtggtt aataacttgt
     2641 ttgtaagcct gtctcttttg ttaaaaaaca ctttgccacc cattggggtt aagagctggg
     2701 ctattcttac ctttgtgggc taagaaaaat gatatggcag aaaagaaccc tacaggcagc
     2761 aggaagacca gggtggctaa acccctcgcg gcagaaatgg gcaaggtgcc cccgcaggca
     2821 attgaacttg aagaagcagt gctgggcgct attatggtgg aaaaggatgc tgttgtcata
     2881 gtcagcgaaa ttcttaaacc cgagtgcttc tataaggaga cccaccagga aatttttaag
     2941 gccatacagg atttatcatc caggttggag cccatcgacc tgtttacggt taccgaggag
     3001 cttaggaggc agaacaagtt ggaggagatt ggaggagccg cttttctggc acaacttacc
     3061 accaaggtgg ggtcagcagc ccatctggaa taccatgcta agattattgc ccaaaagttt
     3121 attcagcgcg agctgatacg ggtttcaacc gatattcaaa acaggtcgtt cgatgaagat
     3181 gtggacgttg atgagctgct cgattactcc gaaatggagc tgtttaaagt tgcagaggga
     3241 aacattaaaa gcgatacgca acccattacc accatcataa aaaaggcaat taagcagatt
     3301 gaggaagcgg ggaagaggga agacgggtta agcggtgtgc cttcgggctt cacagcgctc
     3361 gacaggctta catcgggatg gcagaattcc gacctcatta tcgtagccgc ccgtccatcc
     3421 atgggtaaaa ctgccttcgt tctttccatg gccaggaaca tggcggttga ccacgacgtt
     3481 ccggttgcgt tcttctcgct tgagatgtcg aacctgcagc tggtgaacag gcttattgtt
     3541 agcgagtcgg gaattgcgtc cgaaaaaatc cgcaatggcc ggatcgacca aagcgagtgg
     3601 actcagctta ccgttaaaat taaaaatctt tcggaagcca aaatctttat tgacgataca
     3661 cccgcacttt cccttttcga gtttagggca aagtgccgac gactcaaggc acagtatgat
     3721 gtgggcgtga taattattga ctacctgcag cttatgaccg gcccttcaga aaccaagggc
     3781 aatcgtgagc aggaggtaag caccatttcc cgctcattga aggcaattgc caaggagcta
     3841 aacgttccca ttattgccct ttcgcagctc aaccgctcgg ttgaaacacg agggggatat
     3901 aaacgaccgc agctctccga tcttagggag tcaggtgcta ttgagcagga tgccgacgtg
     3961 gtgattttca ttcaccggcc ggagtactat ggttttatgg aggatgagga tggaaattcc
     4021 acccagggga ttgccgaaat tattgtggcc aagcatcgta atggccctgt tgatgatatt
     4081 aagcttaact tccgtaagga gatggcgaaa ttttccgatt tcgacgacgt gcactatggc
     4141 gatattcagc cctcgcattc acctttcacc tcgtcggtgc ccgtaacctt cagctctaaa
     4201 atgaacgagg atacccacgg ggattatgat tttctctccg ggcaaggggt tgatagcagc
     4261 gatattcctt tttagttgaa ctagtagcgt atcaagagag gatgtaggtg tagagttttt
     4321 ttgacgatta attgatatgc cccagatttg caactcattt tttctgcaaa agaaaatttg
     4381 ctgctttttc ccgctttaga gtatttgggt gatttcctta agggtttcca cctcgtaggt
     4441 tactttttcc ccgtgcggtt tcctttctgg gttgaagaag atttggtcca tcccaaactt
     4501 cctggcccca actatatcta cctctaaatc gtctcccacc ataatgccct gattttttct
     4561 gatatttgca ctggaaagtg cataaccgaa ggcgcgtggg tcgggtttat ggtagcccga
     4621 aag
//
LOCUS       DAOOQX010000065         4608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_22277_length_4608_cov_2.894575, whole genome shotgun sequence.
ACCESSION   DAOOQX010000065 DAOOQX010000000
VERSION     DAOOQX010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22277_length_4608_cov_2.894575"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..575
                     /locus_tag="PLX53_01765"
     CDS             204..575
                     /locus_tag="PLX53_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOY71838.1"
                     /translation="MSMEITGKLLQKLNRQTGEGKNGAWVKQEFIIETLEQYPRKICI
                     AAWGTRVDDLDRLQPGNILKISVNVESREFNGRWYTDVKAWRIEKQDDEAPQSDGGKP
                     GKEDMNLPPESSKEYFDDLPF"
     gene            732..2027
                     /locus_tag="PLX53_01770"
     CDS             732..2027
                     /locus_tag="PLX53_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOY71839.1"
                     /translation="MEVLQVEKIEEQIARKYDFFENLEAWLANQGFSENFAQLLKVGI
                     SVGVIILLALLTDYIAKRIFLSTMQRVAKRTTSEWDDVLVEKKFFQHLAHFAPAAVVY
                     FTVGIALYDYTPKITLIIQALAKIYMVIVGVLVFNSFFDSVNTIYQKLPFAKSRPIKG
                     YLQVVKIILYVFAGIIVIGIIINRNPGTILVGLGASTAVLMLVFKDTIVGLVASIQLS
                     ANNMLKIGDWIEMPGRNIDGTVTDVSLTTIKVRNFDNTISTVPPYALVTESFRNWRGM
                     EESDGRRMIRSIFIDAKTIKFCSPQLLEKLKEVSYCHDWVVEQGEDELKRANEDIAML
                     NKGIPTNLYLYRRYLEGYIKSHPEVNQELPLVVRLKQQVEYGIPVEMYCFLKDKSWVP
                     YETTQSDIVDHALAALPVFELRTFQRMTSDDIRPTAGRS"
     gene            2611..>4608
                     /locus_tag="PLX53_01775"
     CDS             2611..>4608
                     /locus_tag="PLX53_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY71840.1"
                     /translation="MNCKFLVVLLLAVSLLGLRNLAAQTKNQPAGGRYDLSSFSGSIS
                     GQVLDSLTREGVEFASIAIFRQKDSSLVNGTVTDKNGSFKIDKLSPGQYYVDVKFIGY
                     NARRFQGIAVSPRTPEVNIGKIMIVQAYENLSDVVITGEKRMLQYNLDKKVINVEKDI
                     ASQGGTAIDVLQNVPSVNVDTEGNISLRGSENVNVLIDGRPSNLTSLDELPAQMIESV
                     EVITNPSARYDPDGLSGIINIVLKKKKDPGYNGMASIMLGTGGQYNGTLNFNWNRNKW
                     NIFANYDFRVMDRNFNSIAKRTSFLDSDGSMDSLSFLAQNTSGVRNGAFHSLRGGFDY
                     SLGPKATLSFTGNLNIRDFDSKNLTASNSSNSYSSYSSLEDRNNIGNSGGLGQMYSLN
                     FKKTYDAPGKEWTADLYFSRFGNDDKSNISNNAFINSVPEVSSERSNTDSWSNTLTFQ
                     TDYVRPVGNGGRLEAGVKSTIRKSDSDYQYDIFNTGLNTWDFDSTRSNHFIYDEQIYA
                     AYGIYSNSFGQGKFSYQLGVRVEDQIAKSDQQTTGEVADTNRLNFFPSAHIRWEPNSS
                     NSFQVSYSRRVNRPMTWILNPFLNTADKYNWSMGNPYLEPEFTTSLDFNYGLNFPSTK
                     VSASVYYRDTQNGFSRKLALVDSVTTLSTFINLSHNKSYGVE"
BASE COUNT         1332 a          983 c         1023 g         1270 t
ORIGIN      
        1 atgctttatg ttcctgccga tttggcctat ggtgttaatg ttcgtcccgg tggacctatt
       61 ggacccaacc aggcgcttat ttttgaagtt gagctccttg acattgttaa ggaagaaccc
      121 gctaaaaaat agtatattgt taaacctcgc agcttaccag ccgggtgtgg ttcatccggc
      181 tgtttttcta aattttaagt aacatgagca tggagataac aggaaaactc cttcaaaagc
      241 tgaaccggca aaccggtgaa ggtaaaaatg gagcttgggt aaagcaggag ttcattattg
      301 aaaccctcga gcagtacccc cgaaagattt gtatagccgc atggggaacc cgggttgatg
      361 atcttgaccg gctacagcct ggaaacatcc ttaaaatttc tgttaatgtt gagtcgaggg
      421 agtttaacgg gcgatggtac accgatgtga aggcttggcg catagaaaaa caggacgatg
      481 aagcacctca atcggatggg ggaaaacccg gtaaagagga catgaaccta cctcccgaat
      541 catcaaaaga atatttcgac gatttgcctt tttagcaagc tgcatgctgg tttggcttgc
      601 agctgaagtt cataacgtgc gaataaagca ttgccttaga tttctatagc ctaaccaaca
      661 tcttcattta aaaatatctt ataaccagct acacaacaag caagtagtaa accattaaat
      721 caacttacta ggtggaagtg ttacaagtag aaaaaattga ggagcaaatt gcccgaaagt
      781 acgatttctt cgaaaacctg gaggcatggc ttgcaaacca aggatttagt gaaaactttg
      841 cccagctgct aaaagttggc atttcggttg gggtaatcat tctattggct ctactcaccg
      901 actacatagc caagcgtatc tttttgtcca ccatgcagcg ggttgccaag cgcaccacca
      961 gcgagtggga cgacgtgctg gttgagaaaa agtttttcca gcacctggca cattttgctc
     1021 cggctgctgt agtttacttt accgttggaa ttgccctcta cgattacact ccaaaaatca
     1081 ccctgattat tcaggcgcta gctaaaattt acatggttat agttggcgtt ctggttttta
     1141 actcattttt tgattcggta aacaccatat accagaagtt gccttttgct aaaagtcgcc
     1201 ccattaaggg ctacctacag gttgtgaaaa ttattctata cgtttttgcc ggaataatcg
     1261 ttataggtat catcataaac cgcaaccccg gaactattct ggtaggatta ggtgcctcaa
     1321 cggctgtgct gatgctggtg tttaaggata ctatagttgg gcttgtagct tccatacaac
     1381 tttcggccaa caacatgctt aaaattggtg attggattga aatgccaggc cgcaacattg
     1441 atggaacagt tacagacgtt agtctaacca ccataaaggt aagaaacttt gacaacacca
     1501 tttcaactgt tccaccctat gcgcttgtta ccgagagttt ccgaaactgg aggggtatgg
     1561 aggaatcgga tggtcgaagg atgattcgtt ccatttttat tgatgccaaa accattaagt
     1621 tttgttctcc ccagctgctt gaaaagctta aagaagttag ctactgtcat gattgggttg
     1681 ttgagcaggg tgaagatgag cttaaaagag ctaatgaaga tattgccatg ctaaataaag
     1741 gtatcccaac aaacctgtac ctttatcgta ggtacttgga gggttacata aaatcacatc
     1801 ccgaagtcaa ccaagagcta ccactggttg ttcgcctaaa gcaacaggtg gagtacggta
     1861 tccctgttga gatgtactgt tttctgaagg ataagagttg ggttccctac gaaactaccc
     1921 agtccgatat tgttgaccac gccctggcag cactacctgt ttttgagctc cgaacttttc
     1981 agcgcatgac gagcgatgac atccgcccta ctgctggtag gagctaatca aggctatagc
     2041 taagcttttc tattcctgtt ataggagcaa attttgaacc tctccttatc agccttaccc
     2101 gtttgattgc tatggttgca atgccagagg tagattgctg cgctttttct acaagcaaat
     2161 attatatttt gatgacagta agcagcgatt tagtattttt ctacctctta cattcggatg
     2221 aaccccaaaa ggcttgccgt aggctgacac tcataggaag atttcgccga acccacaaat
     2281 aacatccggt tgtttatccg tttttgtgat agctgtccta cattggcgct tatactgtat
     2341 gaaaccaacg ccactctcca ttgtagctat taccagccgt agcatctaac cctgttttaa
     2401 agaatatttt actacaccgc aagtttaacc aaacaatggt gtcaaattaa tgttttgttg
     2461 gagcatatta aaacaaaggg caattatcaa cacccggcaa atttcaaaat gtgaaactat
     2521 attaaaattg cctggcgatg ctaaactcct gagaggggat atgctcaaat gagttgcaaa
     2581 tgtttcatat tttactaaat taattaacac atgaattgta aatttttagt agttcttcta
     2641 ctcgcagtct cattgttggg tttgcgaaat ttagctgctc aaacaaaaaa tcaaccggca
     2701 ggtggccgat atgatttgag tagtttttca ggatcaataa gcgggcaggt gcttgattct
     2761 ctaacccgtg aaggggttga atttgccagt attgccatat ttcgccaaaa ggactcttcg
     2821 ttggtgaacg gaactgtaac cgataaaaac gggagtttta agattgacaa gctgagtccg
     2881 ggtcagtact atgtggatgt aaaatttatc gggtacaatg cccgcagatt tcagggtata
     2941 gctgttagcc cacgtactcc tgaggttaac attgggaaaa ttatgatagt gcaggcatac
     3001 gaaaacctta gcgatgtagt gattacaggc gaaaagcgta tgcttcagta taaccttgat
     3061 aagaaggtaa ttaatgttga aaaggatatt gccagccagg ggggaactgc cattgatgtt
     3121 ttacaaaatg ttccatcggt taatgtggat acagaaggaa atatcagcct acggggtagc
     3181 gaaaatgtta acgttcttat tgatggtcgc ccttctaacc taaccagcct cgatgaactg
     3241 ccggctcaga tgattgagag cgttgaggta attaccaacc catctgcccg atacgacccc
     3301 gatgggctct cgggtataat taacatcgtg ctcaagaaaa agaaggatcc ggggtacaac
     3361 ggtatggcaa gcataatgct aggtactggt ggacagtata atggaacctt gaactttaac
     3421 tggaacagga acaagtggaa catttttgca aattacgatt tcagggtaat ggacagaaat
     3481 tttaactcca ttgccaaacg aacatccttt cttgacagcg acgggagcat ggattcgctt
     3541 tcttttcttg ctcaaaatac tagtggagta aggaatggag catttcatag tcttaggggt
     3601 ggattcgact actccttggg tccgaaagcc accctttcgt tcacagggaa cctcaatatc
     3661 agggattttg acagcaaaaa cctcactgcc tcaaatagta gcaatagcta cagctcatac
     3721 tctagtttag aggacaggaa taacattgga aactcaggtg gtttagggca aatgtactcc
     3781 ctcaacttca agaaaactta tgatgcgcct ggtaaagaat ggactgctga cttatatttc
     3841 tccaggtttg gtaacgacga caaaagcaac atcagcaaca acgcctttat aaacagcgta
     3901 cctgaagttt cgagcgaaag gtcaaacacc gacagctggt ctaatacgct aaccttccaa
     3961 accgactatg ttcgccctgt tggaaatggc ggcaggctgg aggctggagt aaagagcacc
     4021 attcggaaaa gcgattccga ttaccagtac gatattttca atacagggtt aaatacctgg
     4081 gattttgatt ctacacgttc caaccatttt atttacgatg agcagatata tgcagcctac
     4141 ggcatttact ccaacagctt tggccagggc aaattcagct accagcttgg tgttcgcgtg
     4201 gaggatcaaa ttgccaagtc cgaccagcaa actacaggcg aggtggctga taccaaccgg
     4261 ttaaactttt tccctagcgc ccatattagg tgggagccta attccagcaa cagctttcag
     4321 gtgtcgtaca gccgaagggt taaccggcca atgacatgga tactcaaccc gttccttaac
     4381 actgcggata agtacaactg gtcaatgggt aacccctacc ttgaacccga gttcaccaca
     4441 tcgctcgatt ttaactacgg acttaacttt ccttcaacca aggtaagcgc ttcggtttac
     4501 taccgcgaca cccaaaacgg attttcccgg aagctggcct tggttgactc ggttaccacc
     4561 ctttctactt ttattaacct gtcgcataat aaaagttacg gggttgag
//
LOCUS       DAOOQX010000066         4559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_22578_length_4559_cov_2.851465, whole genome shotgun sequence.
ACCESSION   DAOOQX010000066 DAOOQX010000000
VERSION     DAOOQX010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22578_length_4559_cov_2.851465"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PLX53_01780"
     CDS             <1..718
                     /locus_tag="PLX53_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOY71841.1"
                     /translation="NGDIEAVWKYFNNPTGYLYSQQEIEKLATIVKKHDLYLFSDEVY
                     REFCYDGNTHFSVMRLTGLDNNIVLIDSVSKRYSMCGVRIGALISRNKEVIATAMKFA
                     QARLSPPSFGQIAAEAALNTPPEYFKEVYDEYINRRNFMVDALNKMEGVYCPKPKGAF
                     YSVVKLPIDDSDKFAQWLLESFEYKNQTVMVAPATGFYSTKGLGKNEVRIAYVLNTED
                     LRNAMECLEVALKQYPGRKL"
     gene            946..2121
                     /locus_tag="PLX53_01785"
     CDS             946..2121
                     /locus_tag="PLX53_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOY71842.1"
                     /translation="MKFFPTILLIFNFLAATSQDKTTVEAGSEQVPEYLPEILGRNVA
                     VVANHTSLVKGVHLVDTLLSSGVKVRRIFSPEHGFRGNFEAGIPVASSTDYQTGLPVV
                     SLYGGSKQPTKEQLRDIEVVLFDLQDVGVRFYTYLSTLHYMMEACAINNVRLIVLDRP
                     NPNGFYVDGPVLDTANRSFVGLHPVPLVYGMTVGEFALMINGEGWLSKSRKCNLSVIP
                     CKGYSHRITMPLPVKPSPNLPNHLAVLLYPSLGLFEGTAVSVGRGTDYPFQMFGYPAF
                     PLRNFWFLPVEKKGASTNPPYKGKKCYGIDLRDYPVEYFLGKKSINIEWLVYAYNTYP
                     TKSKFFNSFFRLLAGTDELQRQIEGGMSTDEIRRSWSDDLAKYRLIRKKYLLYSDFD"
     gene            2559..3191
                     /locus_tag="PLX53_01790"
     CDS             2559..3191
                     /locus_tag="PLX53_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HOY71843.1"
                     /translation="MKSKNSYYVVWAGYNPGIYGSWEECQQQIKGFPNAKYKGFPTKA
                     GAEKAFSISYEKYMAMNPAQEAIFQIDENVPSPVYPSLAVDAAWNTATLEMEYRGVDA
                     KTGKEIFHQGPFPDGTNNVGEFLAIVHALALLNQKGSTIPIYTDSMNAIAWVRAKTAR
                     TKLEKTPRNQILFDLIQRAEKWLRTNQWSNPLLKWETHVWGEIPADFGRK"
     gene            3234..4475
                     /locus_tag="PLX53_01795"
     CDS             3234..4475
                     /locus_tag="PLX53_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY71844.1"
                     /translation="MRTENYIARRFFKGTNGMSLSRPFIRITITSVALSIAVMIVAVA
                     VITGFKQEIKAKVAGFGSHIQITNYDSNQSLETLPLPSNLKCVEDLKAIKGVRHIQVF
                     GIKAGIIKTPSDMQGVALKGVSSDFDWSFFRQNLVEGSIISVSDSTPTNQTLISKTLA
                     NLLRLKVGDSYDVFFVQEPPRFRRFVVEGIYDTKMVEFDKLFIICDIGHIRRLNGWSS
                     NQVSGYEVLVDNIEKVDDIAIRVDDAVGYDFLDDGSRLKVQSLTERYSSIFDWLELQN
                     INAAVLIILMLLVAGINMISGLLIIILDKTGTIGLLKALGTNNESIRKIFLLQSAYIM
                     IRGLAIGTIAGIAICLLQKYLGIVKLDEEYYFLSVVPINMVWWHIVLLNLASFIVGLA
                     MMVIPSMVVSRISPDKTLKFD"
BASE COUNT         1277 a         1084 c         1051 g         1147 t
ORIGIN      
        1 caatggtgac atcgaagccg tttggaaata tttcaacaac cccaccggat acctttactc
       61 ccaacaggaa attgagaagc tggcaaccat tgttaagaag cacgacctct acctattctc
      121 cgatgaggtg taccgtgaat tttgctacga cgggaatacc catttttcgg tgatgaggct
      181 caccgggctt gataacaaca ttgtcctaat tgattccgtt tccaaacgct acagcatgtg
      241 tggcgttcgg attggtgcct taatttcacg taacaaggaa gtgattgcaa ccgccatgaa
      301 atttgcacag gcacggcttt caccgccatc cttcgggcaa attgctgccg aagctgccct
      361 caacacgcca ccagagtatt ttaaggaggt atatgacgag tatatcaacc gtcgtaactt
      421 catggtggat gcccttaaca agatggaggg ggtttactgc cccaaaccaa aaggagcctt
      481 ctacagcgtt gtgaagctgc caattgacga cagcgataag tttgcccagt ggctcctcga
      541 aagttttgag tataagaacc aaaccgtcat ggtagctcct gcaaccggtt tttactccac
      601 aaaaggattg ggtaaaaacg aggtgcgtat tgcctacgtg ctgaacaccg aggatttgag
      661 aaatgccatg gaatgtctcg aggtagccct taagcagtat cccggtcgta agctttagca
      721 gaacttgaaa taagtgcaaa ataaagggtg gcctgcggaa ccaccctttt tgtgctttat
      781 aaaccatgta cttcagcttc actaggcgga atcaaaaaat atttctactt tagcacaagg
      841 gttttaaact tggcatttaa ggtatcctat ccaaatgcat atctacgcta ccctaagcat
      901 tccggactat ttaaactatc agaatcagca acaactcgcc atcctatgaa gttttttcca
      961 accatattgc ttattttcaa ctttttggcg gcaacttcac aggacaaaac taccgttgaa
     1021 gccggatcgg agcaggtgcc ggagtacctt cccgaaatct tggggcgaaa cgttgctgtg
     1081 gtggccaacc acacctcgct ggtgaaagga gtgcacctgg ttgacaccct gctgtcatca
     1141 ggtgtaaagg tgaggcgaat tttcagcccg gagcacggtt tccgtggtaa ctttgaagcg
     1201 ggaatacctg ttgcaagcag taccgactac caaacaggac tgcctgttgt ttcgctctat
     1261 ggtggtagca agcagcccac caaggagcaa cttcgcgata ttgaggtggt gctcttcgac
     1321 ctgcaggatg ttggcgtgag gttctacacc tacctatcaa ccctacacta catgatggaa
     1381 gcatgtgcca tcaacaacgt caggttaata gtgcttgacc ggcctaatcc caatggattt
     1441 tatgttgatg gccctgtgct tgataccgcc aaccgttcgt ttgtagggct tcatcctgtt
     1501 ccgctggttt atggaatgac tgttggtgag tttgccctga tgataaacgg cgagggatgg
     1561 ctttccaaga gtcgtaaatg caacctgagt gttatcccct gcaaaggata cagccaccga
     1621 ataaccatgc cccttccggt aaaaccatca ccaaatttac ccaaccactt ggcggtgctg
     1681 ctatacccat cgctaggtct ttttgaagga actgccgtca gcgtgggcag gggaaccgat
     1741 tacccatttc aaatgtttgg atacccggct ttccccctca ggaacttctg gtttttaccg
     1801 gttgagaaaa agggggccag cactaatcca ccctacaagg gtaaaaagtg ttacggcatc
     1861 gacctcagag actatcctgt ggagtacttt cttggtaaaa aatcaataaa tatcgagtgg
     1921 ctcgtttacg cctacaatac ctatccaacc aagtcgaagt tttttaacag cttcttcagg
     1981 ctgcttgccg gcaccgacga gctgcaaagg cagattgaag gcggtatgag caccgacgaa
     2041 atccgcaggt catggtcgga cgacctggct aagtacaggt tgatcagaaa aaaatactta
     2101 ctctactccg attttgacta gctttttcaa tcaaactttt aaaatatggg aatatagcat
     2161 actgcttata tagatattgc cgaattttac tgataaactc tgtcctattc tgtatcatcc
     2221 ctcatatccg tgatacatta aaagttttta ttcatcagcg tattaatcag aaatcatcat
     2281 gtacctttga aaggtaaaac cgaaagaaat aagtcaacct tgataaaagc cacttttcaa
     2341 attatcatgt tgtttcccaa tggtttgttt catggcataa gcatcaagct aaaaaaccaa
     2401 tgaaacacca atgcaatgca gcttgcacaa acgtgcatga gcaagcggca gttacctgcg
     2461 gattcggcga ggccaaaata tgggtgttcc atccgctcgg ccgcttggca caaaggtatg
     2521 ggcaagtatt tgtaatctta acctaaaaat tatgcactat gaaaagcaaa aatagctact
     2581 acgtggtgtg ggctggttac aacccgggca tttacgggag ctgggaggag tgccagcaac
     2641 aaattaaagg gttcccgaat gcaaaatata aaggttttcc tactaaagct ggtgcagaga
     2701 aggcttttag cataagctac gagaagtaca tggcaatgaa tccggcacag gaggccatat
     2761 tccaaattga tgaaaacgta ccaagccctg tttacccttc gctggcggtt gatgcggcat
     2821 ggaataccgc aacccttgaa atggagtata ggggcgtgga tgccaaaacc ggaaaggaaa
     2881 tattccatca gggacccttc ccggacggga caaacaacgt gggggagttc ctggcaattg
     2941 tccatgcgct ggccctgttg aaccagaaag gcagcaccat cccaatctac accgattcca
     3001 tgaacgccat tgcctgggta cgggctaaga cggccagaac aaagctggag aaaactcccc
     3061 gtaaccagat actattcgac ttgatacaaa gggccgaaaa gtggctaaga acaaaccagt
     3121 ggagcaaccc cctgctgaaa tgggaaaccc atgtgtgggg tgagatacct gccgatttcg
     3181 gcagaaaata gcacaaatcc tacccaactt attccataaa gaaatttcaa cctgtgcgca
     3241 ccgaaaacta catagcccga cgattcttta aaggcacaaa cggcatgagc ctgtccaggc
     3301 cttttatccg tataaccatt acctctgttg cccttagcat tgcagtgatg atagtagcag
     3361 tagccgtaat aaccgggttc aagcaggaga taaaggccaa agtagccgga tttggtagcc
     3421 atatccagat aaccaactac gacagcaacc agtcgcttga gaccctcccg ttaccatcga
     3481 atctaaagtg cgtggaggac cttaaagcga ttaagggggt taggcatatc caggtttttg
     3541 gtattaaggc cggtattatt aaaacaccct ccgatatgca gggagtggcg cttaaaggag
     3601 tttcctccga ttttgactgg tcgtttttcc gtcaaaacct ggtggagggc agcattatta
     3661 gcgttagcga ctccaccccc accaaccaaa ccctcatatc caaaacactg gccaacctat
     3721 taaggctaaa ggttggcgac tcctacgacg ttttctttgt tcaggaaccc ccacgtttcc
     3781 gacgtttcgt agtagaggga atctacgata ccaaaatggt ggagttcgac aagctattta
     3841 tcatctgcga cattgggcat atccgccgtc ttaacggatg gagcagcaac caggtaagcg
     3901 gttacgaggt gctggttgac aacattgaaa aggtggatga catagccatc cgggttgacg
     3961 atgccgtggg atacgatttt ctcgacgatg ggtcacgatt aaaggtacaa agcctgaccg
     4021 aacgctactc cagcatattt gactggctgg aactgcaaaa cataaatgca gcagtgctca
     4081 tcatactcat gcttctggtt gccggcatca acatgatttc gggtcttctc atcatcatcc
     4141 tcgacaaaac gggaactatt ggcctgctaa aagcgctcgg aacaaacaac gagagcattc
     4201 gcaaaatttt cctcctacag tcggcctaca tcatgattcg cggtttggct ataggcacaa
     4261 ttgccggaat tgccatttgt ttgttacaaa agtatttagg aattgttaag ctcgacgagg
     4321 agtactactt cctgtcggta gtgcccatca acatggtgtg gtggcacatc gttttactca
     4381 atctggcttc atttattgtt gggcttgcca tgatggttat accctccatg gtggtttcgc
     4441 gtataagccc cgacaaaacg cttaagttcg actagcatgt cgtaaccatt acaaaggtgg
     4501 cctaacccgc ataattccct cttaaccgaa gattggaaaa tgttgttggc ggaataggc
//
LOCUS       DAOOQX010000067         4552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_22626_length_4552_cov_3.263287, whole genome shotgun sequence.
ACCESSION   DAOOQX010000067 DAOOQX010000000
VERSION     DAOOQX010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22626_length_4552_cov_3.263287"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLX53_01800"
     CDS             complement(<1..195)
                     /locus_tag="PLX53_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71845.1"
                     /translation="MLRIAELICLFVLVPLAFYFDFIPLPKFVPLLVVFIYALVFLLR
                     SKTFSRKEFGLNGYRSWPGLI"
     gene            complement(400..1617)
                     /locus_tag="PLX53_01805"
     CDS             complement(400..1617)
                     /locus_tag="PLX53_01805"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY71846.1"
                     /translation="MVQRYKIGIVRETKTPPDRRVALPPNQARELTTRYSALEIAIQP
                     SSIRCFSDEEYARIGIKLQEDLSDCDLLIGIKETKIPTIIPGKTYMEFAHVAKKQPHN
                     RPLIQAFADQGNTLIDYEYLTDDKGNRLIAFGHWAGIVGAYNAIRALYIKEDLPELKP
                     AHQMHDYKEMQEVLKGLKVIPKKFVITGGGRVAGGAMEVLAQAGVKEVTPDEFLKGQF
                     DYPVYCRLDPWHYAKRKDGKPFDWSYWLSNPADHDTAFIPFTSATDILIACHYWDYRS
                     PHFFSREDMLNNSFRISIIADVSCDIPGPIPSTIKASTIADPFFDYNPKTDMEEPPFS
                     SKGNVTVMSIDNLPGELPRDASEFFGNILIEKIIPHIVTGDKEGIIERATILKNGKLT
                     GKFSYLSDYLKGE"
     gene            1926..>4552
                     /gene="nifJ"
                     /locus_tag="PLX53_01810"
     CDS             1926..>4552
                     /gene="nifJ"
                     /locus_tag="PLX53_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOY71847.1"
                     /translation="MAKSKKFITCDGNYAAAHIAYMFSEVAAIYPITPSSTMAEYVDE
                     WAAFGRKNIFGDTVRVVEMQSEAGAAGAVHGSLQSGALTSTFTASQGLLLMIPNMYKI
                     SGELLPGVFHVSARSIAAHALSIFGDHSDVMSTRQCGFAMLATSSVQEVMDLAGVAHL
                     SAIKSRIPFLHFFDGFRTSHEIQKIEVLENDDLAPLLDQKSLQEFRDRALNPEHPVTR
                     GTAQNPDIYFQAREASNKFYMDVPDIVEGYMQEITKLTGREYHPFSFYGDPNAENIII
                     AMGSVTDTIRETIDYLMAKGEKVGMISVHLYRPFSAKYFFKVLPSAVKRIAVLDRTKE
                     PGANGDPLYLDVKELFYGREKAPIVVGGRYGLSSKDTTPAQIIAVFDNLKLSEPKNQF
                     TIGIIDDVTFKSLPTGKEVNLAPKGTFQAKFFGLGSDGTVGANKNSIKIIGDNTPKYC
                     QAYFAYDSKKSGGITVSHLRFGDQPIRSPYLVITPDFVACHVQSYLGKYDVLKGLQPG
                     GTFLLNTIWSKDELNEKLPNSIKRYMAENSIKFYIINATQIAEEIGLGNRTNTIMQSA
                     FFKISGVIPYDVAVKEMKKAIEKSYGRKGEEVLRMNFAAVDRGGDAIQVVVPMEWAKL
                     QPENKLEIEGAPEFINKVVLPINLQMGDDLPVSAFVGREDGTFPAGTTRYEKRGIAVN
                     VPEWISENCTQCNQCAYVCPHAAIRPFLLTEEELKNAPAGTKTVRGNGGIKDFNFRVQ
                     VSVLDCTGCGNCVEVCPSKEKSLVMRPIESQMAEAARFDYMHEKVGYKDILGKEKSVK
                     NSQFAQPLFEFSGACAGCGETPYIKAITQLYGDRMIVANATGCSSIYGGSAPSTPYSA
                     GPDGRGVAWSNSLFEDNAE"
BASE COUNT         1226 a         1004 c         1063 g         1259 t
ORIGIN      
        1 tatcaaccca ggccagctac ggtacccatt aagcccaaac tcctttcggc tgaaggtttt
       61 agacctaagc aggaatacta gagcataaat aaagactacc agcaggggaa caaattttgg
      121 cagtgggata aaatcgaagt agaatgccag tgggaccagc acaaacaaac atataagctc
      181 agcaatacga agcataggaa aaactatcca acttgaggtt taaatttaac agctcagcct
      241 gccaaaagtt tattttaggc ttagtgttgt tcaattatgc tagtttagct ctaagtaaca
      301 ccccacaccg aactaccatt ccttggccca aaagcccgaa tatgccctgt cttaataaag
      361 ataaactgtc caacagcttt gagtaccctt ttttagaggt tattctcctt tcaggtaatc
      421 ggataggtag gaaaattttc cggttagttt tccattttta agaatggtag cacgttctat
      481 tattccttcc ttgtcaccgg taacgatatg tggaataatt ttttctatca ggatattccc
      541 gaaaaattcg ctggcatccc tgggtagctc tccgggaagg ttgtcgatcg acataaccgt
      601 tacattgcct ttggacgaga agggaggctc ctccatgtcg gttttagggt tgtaatcaaa
      661 aaagggatcg gcaatcgttg aggctttaat ggttgatggg atcggcccgg gaatatcgca
      721 gcttacatcg gcgataatcg aaatgcgaaa gctattgttt agcatatcct cacgcgaaaa
      781 aaaatgggga gaacggtagt cccagtagtg gcaggcaatc aatatgtcgg ttgcacttgt
      841 aaagggtata aaagccgtgt catggtcggc cgggttactc aaccagtagc tccagtcgaa
      901 aggctttcca tccttacgtt ttgcatagtg ccatgggtct aaccgacagt aaacagggta
      961 gtcgaactga cccttcagaa attcatcggg ggtaacttct tttaccccgg cttgtgccag
     1021 tacctccata gcgccgccag caactcgccc cccgccggtt atgacaaatt ttttggggat
     1081 gaccttcaac cccttcagca cttcttgcat ctccttgtag tcgtgcatct ggtgggctgg
     1141 ctttagttcc gggagatctt ctttaatgta gagcgctcta atggcgttat aggctccaac
     1201 aataccggcc cagtgcccaa acgcaataag cctgtttccc ttatcatcgg ttaaatactc
     1261 atagtcaatt agtgtattgc cctgatcggc aaacgcttgg attaacgggc ggttatgggg
     1321 ctgctttttg gctacatggg caaactccat gtaggtttta cccggaataa tggtgggtat
     1381 ttttgtctct tttatcccaa tcagcaggtc gcagtccgat aaatcttcct gaagttttat
     1441 gcctatcctg gcatactcct catcggagaa gcaccttatg ctacttggtt ggatagcaat
     1501 ttcaagggct gagtacctgg tggtaagctc cctggcctga tttggtggca gcgctactct
     1561 tctgtcaggt ggcgttttgg tttctcttac aatacctatt ttatatcgtt gaaccatagc
     1621 atttgattta aggtgaaaaa atgttaaaaa aaatcatgaa cctgcgaatc taacaaactg
     1681 taattaaatt tccaataata gcttactaaa ttctgaagta tttctcgttc tgtaaacaag
     1741 tgtaaggtaa tcgtgctatt cgattttaaa ttttacttta tatgaaacgg agtttgttgg
     1801 taatgctgac tttcaacggg catcatattt aaggtgtgtt ataaaacctg gttatagttt
     1861 tttaattgta ttacctttgc aaaaaatttc agaaaagcac taattttctt aataccttaa
     1921 ataaaatggc aaaatcaaag aaatttatta catgcgatgg gaactacgct gcagcccata
     1981 tagcctacat gtttagcgag gttgccgcca tttatcccat tactccttca tctaccatgg
     2041 ccgagtacgt tgatgaatgg gccgccttcg ggaggaaaaa catttttggt gatacggtta
     2101 gggttgttga gatgcaaagc gaagctggag ctgctggtgc tgttcacgga tcgcttcagt
     2161 ccggagcatt aacttcgaca tttacggcat cccaagggct tcttttgatg atacccaaca
     2221 tgtataagat ttcgggagag ctgcttccag gggtatttca cgtttctgcc cgcagcattg
     2281 cagcacacgc cctttcaatt ttcggagacc atagcgatgt tatgagcacc cgtcagtgtg
     2341 ggtttgccat gttggccacc agcagcgttc aggaggtaat ggacctggcc ggagtagccc
     2401 atctctcggc aatcaagtcg agaattccat tcctgcattt cttcgatggg tttagaacct
     2461 cccatgaaat tcagaaaata gaggtgctgg agaacgatga cctagctcca ttgctcgacc
     2521 aaaaatcact tcaggagttt cgcgacaggg cattgaaccc cgaacatccc gtaacccgtg
     2581 gcacggctca aaaccccgat atatactttc aggccaggga ggcttccaac aaattttaca
     2641 tggatgttcc cgatatcgtt gagggctaca tgcaggagat taccaaactt accgggcggg
     2701 agtaccaccc attttcattc tatggcgatc ctaatgccga aaatatcatt attgccatgg
     2761 gttcggttac cgacaccatt cgtgaaacta ttgactacct catggctaaa ggtgaaaagg
     2821 ttgggatgat aagcgttcat ttgtacagac ctttctctgc gaaatacttc tttaaggtgc
     2881 tgccttcagc tgtgaaacgc atagcagtgc ttgatagaac caaggaaccc ggggccaacg
     2941 gcgatccgtt atacctcgat gtaaaagagc tcttctatgg gagggagaaa gctccgatag
     3001 ttgtgggtgg ccgttacggg ctcagctcaa aggatactac accagcccaa atcattgcag
     3061 tttttgataa tctcaagctt agcgagccta aaaatcaatt taccattggc atcattgatg
     3121 atgttacctt taagtcgtta ccaaccggta aagaggtgaa ccttgcacct aaaggaacct
     3181 ttcaggccaa atttttcgga cttgggtcgg atggaaccgt tggggctaac aagaactcca
     3241 ttaaaataat tggtgataat accccaaagt actgtcaggc atactttgcc tacgattcaa
     3301 agaagtctgg aggcatcacc gtttcccacc tgcgttttgg cgatcagcca atccgttcgc
     3361 cttacctggt aattactccc gattttgttg cctgccacgt tcaatcatac cttggtaagt
     3421 atgatgtgct taagggtttg cagcccggtg gaacattcct gctcaacact atctggagta
     3481 aggatgagct gaacgaaaag ctaccaaact ctattaagcg ttacatggct gaaaatagta
     3541 ttaagttcta cataattaat gctactcaga ttgccgagga gattggtttg ggtaaccgca
     3601 ccaacaccat aatgcagagc gcatttttta aaatttcggg tgtaatcccc tacgatgtgg
     3661 ctgttaagga gatgaaaaag gccattgaga aatcatacgg acgtaaggga gaagaggttt
     3721 tgaggatgaa ttttgccgcc gttgatcgtg ggggagatgc tattcaggtt gtagtgccta
     3781 tggaatgggc aaaacttcag cctgagaata aacttgaaat tgagggtgcc cctgagttta
     3841 tcaataaggt ggtgctccca ataaaccttc aaatgggtga tgatttgccc gtgagcgcat
     3901 ttgttggtcg tgaggatgga actttccctg ccgggacaac ccgttacgaa aagaggggaa
     3961 ttgcagttaa tgttcctgag tggatttcgg aaaactgcac ccagtgcaac cagtgcgctt
     4021 atgtttgccc ccatgcagcc attcgtccct tccttttaac cgaggaagag ttgaaaaacg
     4081 cacctgcagg aaccaaaact gttaggggaa atggcggaat taaggatttc aacttcaggg
     4141 tacaggtgag cgttctcgac tgcaccggct gcggcaactg tgttgaggtt tgcccctcca
     4201 aggaaaagtc gctggttatg cgaccaattg aatcacaaat ggccgaggca gccaggttcg
     4261 attacatgca cgaaaaggtg ggctacaagg atattcttgg taaagaaaaa tcggtaaaga
     4321 acagccaatt tgcccagcca ttattcgagt ttagcggagc ttgcgctggt tgtggcgaaa
     4381 ctccttacat aaaggctatt acccagctct atggcgaccg catgattgtg gcaaatgcta
     4441 caggttgttc ctccatttac ggtggctcag ctccatctac cccatactct gccgggcctg
     4501 atggcagagg tgtggcttgg tcaaactcat tgtttgagga caatgccgag ta
//
LOCUS       DAOOQX010000068         4541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_22696_length_4541_cov_3.067766, whole genome shotgun sequence.
ACCESSION   DAOOQX010000068 DAOOQX010000000
VERSION     DAOOQX010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22696_length_4541_cov_3.067766"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLX53_01815"
     CDS             <1..321
                     /locus_tag="PLX53_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71848.1"
                     /translation="IVDAGMNALIRPALYGAYHRIVNTSTSSSKMERYEVVGPLCEST
                     DILGTNVLMPVTQRNDVLEVLSCGAYAEAMSSRINLRDIPDYCFTNQLQEKTITKTGA
                     PLQG"
     gene            530..673
                     /locus_tag="PLX53_01820"
     CDS             530..673
                     /locus_tag="PLX53_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71849.1"
                     /translation="MRKPLILLFVVVTLSLASCYKNNATFTVQVNDYAPTYTVAYTAQ
                     NGV"
     gene            1205..2290
                     /gene="carA"
                     /locus_tag="PLX53_01825"
     CDS             1205..2290
                     /gene="carA"
                     /locus_tag="PLX53_01825"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOY71850.1"
                     /translation="MNKQKVKLVLADGSEYSGYSFGYFGSTAGEAVFNTAMNGYPESL
                     TDPSYRGQVLIATFPLVGNYGVPEGITEGNLHRFWESDRVQISGLVVSVYNEDFSHWN
                     AAKSLGDWLAEYRIPAIAGIDTRALTRKIREKGSMLAKILALNDVEWHDPNSENLVAQ
                     VTVTQKEIYGNGEYRVVLVDCGVKNNIIRCLTQRNTTVVRVPYDYDYTDEDYDGLFIS
                     NGPGNPKMNTATIAVLQKAMKSNRPIMGICLGNQLLALASGADTYKLPFGHRSHNQPV
                     ILKGTNRCFITSQNHGFAIDSSTLSDDWDVLFENANDGTCEGIRHKSKPIFAVQFHPE
                     ASGGPNDTEFLFDEFLQLMVKHKQINS"
     gene            2295..>4541
                     /gene="carB"
                     /locus_tag="PLX53_01830"
     CDS             2295..>4541
                     /gene="carB"
                     /locus_tag="PLX53_01830"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HOY71851.1"
                     /translation="MNSKYHKVLVLGSGALKIGEAGEFDYSGSQALKALREEGVKSIL
                     INPNIATVQTSWGVADRVYFLPVTPYFVERVVEKERPDGIFLSFGGQTALNCGIELFK
                     TGFLAKHNVQVLGTPVETIINTEDRELFARQLSQINVSTPRSQSARCLDDALQAAEKI
                     GFPLIVRSAFTLGGQGSGFCSNAEELRELSINAFSYSPQILVEESLWGWKEVEYEVVR
                     DRFDNCITVCNMENLDPLGIHTGESIVVAPSQTLTNAEYHKLRSLSIKIIRHLGIIGE
                     CNVQFALDPKSEDYRVIEVNARLSRSSALASKATGYPLAFIAAKLGLGYGLHQLKNPV
                     TAITTVCFEPAMDYIVVKIPRWDLNKFSGVSHEIGSSMKSVGEIMAIGRSFEEAIQKG
                     LRMVGQGMHGLVANTKLNFPNLEEALVHPTDMRIFAIAEALELGWTVEKVHELTNIDR
                     WFLGRMQGIITLEKKLMQVASVEMLDRETLLEVKQFGFSDFQLARLIYNPGPDDIETR
                     QQVVRYHRIQLGIVPVAKQIDTLAAEYPSETDYLYLTYHGIENDTSPNQSNRTIVVLG
                     SGAYRIGSSVEFDWCTVSAAGKIRKLGYRAVMINFNPETVSTDYDMCDKLYFDELTFE
                     RVMDIIELEKPIGVMVSMGGQIPNNLAMRLHNAGVKVLGTSPESIDRAEDRQKFSEML
                     DMLHIDQPRWKQLSTLEEVERFVDEVGFPVLIRPSYVLSGSAMNVVSNADELRHYLVL
                     AANVSKTHP"
BASE COUNT         1292 a         1027 c         1082 g         1140 t
ORIGIN      
        1 attgtggatg ctggaatgaa cgcccttatc cgccctgcgc tttacggcgc ctaccacaga
       61 atagtgaaca ccagcaccag cagctccaaa atggaacgct acgaggtggt tggcccgttg
      121 tgtgagtcaa ccgatattct tggaactaac gttttgatgc ctgtaaccca gcgaaacgac
      181 gtgctggagg tactttcgtg cggcgcctat gccgaggcca tgagctcacg catcaacctt
      241 agggatattc ccgattactg ctttaccaac cagctgcagg aaaaaacaat tacaaaaaca
      301 ggagctcctc tgcagggctg atatcaactc aattagctac agttgttgcc tatattctca
      361 aaacccatgt tagtagctat tctataaggt gaatgttgct gtatgttcag tagaatgaac
      421 cgatgtggca tgccactgcg taaggacttg atttggtggt taatattttt gagaaaattc
      481 caagaaattt acctgctatt atcgtcgact aactaaatag gttagcgcca tgagaaagcc
      541 attgatatta ctttttgtgg ttgttacctt gagtttggca tcatgctaca aaaacaacgc
      601 tacatttacc gttcaggtaa acgactatgc gccaacctac acggttgctt acacggctca
      661 aaatggggta tgattgggca gcctttaagt tccgaaaaac tatgagtttt tcaacgagta
      721 ctcctatggc tggcagctaa acctcaaggt taaggcggaa gacagcatat ttgtatatgt
      781 gcaagaacga gggaacctga aagaaccaat tataagtgta aggattcact agaagttcat
      841 aaaatccatg ccattctgaa ccaattaatt catgtgaaat tggatttgaa ttgcatcatt
      901 ttcttagaaa ggtttaattg atccatgtag ctgtttacca gtattgtagc aaaatatgag
      961 tttgcaattt aaggggtggc tatatatttc aaagatttct tttactttta ctccgtaaaa
     1021 cgcagctata tgtttcaggt tgatagaata atactcagca gtaagcacac aggatttgaa
     1081 aattcaacat gcttttgtaa tgaatgtctg cccattccgg cagacttttt ttttacccaa
     1141 cagttggcgg tagctgcagc tgcaaaaacc acatcgcaaa ataactaaac agctaaaact
     1201 atctatgaat aagcaaaaag taaagctggt gctggcagat gggagcgagt attccggcta
     1261 ctcctttggg tatttcggca gcactgcagg tgaggccgta ttcaacaccg ccatgaacgg
     1321 ttatcccgaa agtcttaccg atccctccta ccgtggacaa gtactaattg ccaccttccc
     1381 gttggttgga aactacggcg ttccggaagg aataaccgag ggaaatctac ataggttctg
     1441 ggaatcagat agagttcaaa tatctgggct ggttgtatcg gtatataatg aggatttcag
     1501 ccactggaat gccgccaaaa gtcttggcga ttggcttgct gaataccgaa ttcctgcaat
     1561 tgccggcatc gacacaagag ctttaacccg taaaatacgg gagaagggta gcatgctggc
     1621 taaaatattg gctttaaacg atgtggaatg gcacgaccca aactccgaaa acctggtggc
     1681 ccaagtcacg gtaactcaaa aagaaatcta tggaaatggg gagtacaggg tagttctggt
     1741 ggattgcggg gtaaaaaaca acattatccg atgtttaacg caacgcaaca caacggtggt
     1801 aagagttccc tacgattacg attacaccga tgaagattac gatggcctgt tcatatccaa
     1861 cggaccggga aatccaaaaa tgaacactgc cacaattgcg gttctgcaaa aggcaatgaa
     1921 aagtaatagg ccaattatgg gtatatgcct ggggaatcag ctgctggctt tggcatccgg
     1981 cgctgacacc tataaacttc cctttggaca cagaagccac aaccaacctg taattcttaa
     2041 gggaacaaac aggtgcttca ttacctcgca aaatcatggc tttgcaattg actcctcaac
     2101 cctttcggac gactgggacg tgctttttga gaatgccaac gacggaactt gtgagggtat
     2161 caggcacaaa tctaaaccaa tatttgcagt gcagtttcat cccgaagcgt cgggaggccc
     2221 aaacgatact gaattcttat ttgacgaatt tcttcagttg atggtaaagc ataaacaaat
     2281 caacagctaa ccgtatgaac agcaaatatc ataaagtact ggtgcttggc tcgggagccc
     2341 ttaaaatcgg cgaagccgga gagtttgact acagcggatc gcaggcacta aaagccctga
     2401 gggaggaggg ggttaaaagc atactgataa atcctaatat tgccaccgtt caaaccagct
     2461 ggggagttgc cgatagggtt tactttctac cggtaactcc gtactttgta gagcgggtgg
     2521 ttgaaaagga gcgccctgac ggaatatttt tatcgtttgg aggtcaaaca gcattgaact
     2581 gtggaattga gctcttcaaa acaggatttc ttgctaagca caacgttcag gtgctaggaa
     2641 cgccagttga aaccatcatc aacacggaag acagggagct gtttgccaga caactctcac
     2701 aaattaatgt tagcacgccc cgcagccaat cagcaagatg ccttgatgat gcactacagg
     2761 ccgccgaaaa aattggtttt ccgcttatag ttcggtcggc attcacattg ggaggtcagg
     2821 gaagcggttt ctgctcaaac gctgaagaac tccgggagct ttccattaat gccttttcat
     2881 actcccctca gattcttgtt gaggaatcgc tatggggatg gaaggaggtg gagtatgagg
     2941 tagttcgcga ccgcttcgac aactgcatca ccgtttgcaa catggagaac ctcgaccccc
     3001 tgggtatcca tactggtgaa agtattgtag ttgcaccctc gcaaaccctt accaacgctg
     3061 aataccacaa gttacgctca ctttccatta aaatcataag acatctgggc attattgggg
     3121 agtgcaacgt tcagtttgcc ctcgatccga aaagtgagga ctaccgtgta attgaggtaa
     3181 acgcccgtct gtcgcgctcg tcagcacttg cctcaaaggc aacaggctac ccacttgcct
     3241 ttattgctgc aaaactcggg ttgggctacg gccttcatca gcttaaaaac cctgtaacag
     3301 ccattaccac tgtctgtttt gaacctgcaa tggattacat cgtcgtaaaa attccccgat
     3361 gggatttgaa taagttttcg ggcgtcagcc acgaaatagg ctcatccatg aaaagtgtag
     3421 gagagataat ggccattggc cgctcctttg aggaggccat tcagaagggt ctcaggatgg
     3481 ttggacaggg catgcacggg ctggtggcca acacaaagct caactttccc aacctggaag
     3541 aggccctggt ccaccctacc gatatgcgaa tttttgccat tgccgaagca ttggagctgg
     3601 ggtggacggt tgaaaaagtt cacgagctga caaatattga caggtggttc ctcggaagaa
     3661 tgcagggaat cattaccctt gagaaaaagc tgatgcaggt tgcatcagtt gaaatgctgg
     3721 atagggaaac cctgcttgag gtgaagcagt ttggcttttc cgactttcag ctggccaggc
     3781 tgatatacaa tcctggtcca gatgatatcg aaacccgcca gcaagtggta cgataccaca
     3841 ggatacaact tggtattgtt cctgtggcca agcaaataga cacgcttgca gccgaatatc
     3901 cttccgaaac ggattacctc tacctgacct atcatggtat tgaaaacgac acatcaccta
     3961 accaaagcaa ccgaaccata gttgtacttg gttcgggagc ctaccggatt ggcagctctg
     4021 ttgagtttga ctggtgcacg gtaagtgcag ccggcaaaat aaggaagctt ggctacagag
     4081 cagtaatgat caacttcaat cccgaaacgg ttagcaccga ctacgacatg tgcgacaagc
     4141 tgtactttga tgagctaacg tttgaacggg ttatggacat cattgagtta gaaaaaccta
     4201 ttggtgtaat ggtctccatg ggaggccaaa tccccaataa cctggccatg aggcttcata
     4261 atgcaggagt aaaagtattg ggaacttcgc ccgaatccat agatagggct gaagatcggc
     4321 aaaagttctc cgaaatgctc gacatgctgc acatcgacca gccccgatgg aagcagctat
     4381 ccaccctcga ggaggttgaa cgttttgttg acgaggtggg cttcccggtt ctcatacgtc
     4441 catcctacgt gctgtcgggc tcggcgatga atgtagtttc caacgccgat gagcttcgtc
     4501 actacctcgt tctggcggct aatgtatcca aaacccaccc g
//
LOCUS       DAOOQX010000069         4532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_22754_length_4532_cov_2.862184, whole genome shotgun sequence.
ACCESSION   DAOOQX010000069 DAOOQX010000000
VERSION     DAOOQX010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22754_length_4532_cov_2.862184"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLX53_01835"
     CDS             complement(<1..401)
                     /locus_tag="PLX53_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71852.1"
                     /translation="MKPFQMFVFITIALGVYLLVNLFIYYQTRVVFSIPRWGILLKII
                     FLALVLAYPVGRGLEAIFDGNTFTPLVRIGSMWLGAMLYLFMAFLVVWIVVVSTHTFG
                     SANPIHARQVGVALVYALVSILLIAGYTNAIT"
     gene            complement(699..1109)
                     /gene="rnk"
                     /locus_tag="PLX53_01840"
     CDS             complement(699..1109)
                     /gene="rnk"
                     /locus_tag="PLX53_01840"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HOY71853.1"
                     /translation="MSRITLNRLDYERIKRHLEVARQLKSITNTEADKLLGELESAQV
                     VNPESIPSDVVTMNSIVKISFLNTGKEIQFRIVYPHEANLKENKISIFSPIATALIGY
                     RVGDEIEWLVPAGPTKIRIEEIVYQPEASGNFTL"
     assembly_gap    1504..1603
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1643..1716)
                     /locus_tag="PLX53_01845"
     tRNA            complement(1643..1716)
                     /locus_tag="PLX53_01845"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1680..1682),aa:Arg,seq:tct)
     gene            complement(2201..2659)
                     /locus_tag="PLX53_01850"
     CDS             complement(2201..2659)
                     /locus_tag="PLX53_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71854.1"
                     /translation="MTTKNKIDKMFGPVGTLAGIFLFVAGSIITYFSIAGLVVVLIGA
                     FVGFTSTITSIDFGKKRLRFSIYIFGLFPIGKWILIQSDMKIGIKKSTKLWRTYSQSN
                     RTLDIADNDYRLILYDSNGKKIMPIQKSGNLNSAKFNLDKLSKQLEIGVI"
     gene            complement(2843..3175)
                     /locus_tag="PLX53_01855"
     CDS             complement(2843..3175)
                     /locus_tag="PLX53_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71855.1"
                     /translation="MDISEQYNDLTARITDSLPLKAFPIRELVQIFRKNGHPITLKTE
                     LKITGVYNSGDTSGIMCTVEIADGYKMACALTHLIFLRSDKLYSDIMDYQRERVKRIK
                     QLNLTGLN"
     gene            complement(3187..3501)
                     /locus_tag="PLX53_01860"
     CDS             complement(3187..3501)
                     /locus_tag="PLX53_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71856.1"
                     /translation="MDTNDLSRETHTAIIDTAERFHHDLTLQFGVLAGDCNTDNEFLD
                     ESELMIKEWLTDWDLEEVIMDIFFDDTPRKQDFKKVLDKILTNIEKVRKIPIEQRKFD
                     IW"
     gene            complement(3676..4227)
                     /locus_tag="PLX53_01865"
     CDS             complement(3676..4227)
                     /locus_tag="PLX53_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71857.1"
                     /translation="MPHIMKMRLFYMIIPILLSCSTAVKIKKSNIENISKTKYKSTCG
                     IVLEKDYVYLFPDEFNGTRFTPNEEDILKAEIILKNKIKETNTPNMMNQGDNCPIIHK
                     NLKKYFRQYSGELNTKNEKIIYINMLWNRFPFWHKVLGYEDQRDKYETGYYFVLDGCS
                     YYWQISINISTEKIELFTVNGVA"
BASE COUNT         1259 a          869 c          850 g         1454 t
ORIGIN      
        1 gttatggcgt tggtgtaacc agctattagc agaattgaaa ccaaagcgta aaccaaggcc
       61 acacctacct gccgggcgtg aatcggatta gccgatccaa aggtatgggt tgaaaccact
      121 actatccaaa caacaaggaa agccatgaac aggtatagca tggcacctag ccacatggag
      181 cctatacgaa caagcggggt aaatgtattt ccatcaaaaa tggcttctag ccctctccct
      241 acggggtagg ctagcaccag ggcaagaaag ataattttta gcaaaatacc ccatcggggt
      301 atggagaaaa ccacccgggt ttgatagtaa atgaaaaggt ttaccagcag gtaaactcca
      361 agggcgatgg taatgaatac gaacatctga aatggcttca tccgtttaaa ttatttactg
      421 caagtgtgag ctaatctact gttctgcccg cgaaaatacg ttgcataaac atataattgc
      481 attcaatgtt caccgtattc ataaaacatg ctgaatcata gctatttgta atcaagtaga
      541 tttatcggca acctaaggca cagtaggcat aaacaggctc cagggttcca cccggagcga
      601 aattttacgg gcagctgaat catacatagc aagccctgcc tgatactgga atgaatttgc
      661 ttacaggcag ggagtacaaa gtttacattc aatttcgctt aaagggtaaa gttgcccgag
      721 gcttcaggtt gatagactat ctcctcaatt cgtattttgg taggaccggc gggcaccagc
      781 cattcaatct catcgcctac cctatagcca attagcgctg tggcaatggg tgaaaatata
      841 gagattttgt tctcctttaa gttagcttcg tgggggtaca caattctaaa ttgaatctcc
      901 ttgccggtgt tcaaaaagct gattttgaca attgagttca tggtaacaac atctgaggga
      961 atgctttctg ggttaactac ttgggccgac tccagctccc caagcagctt atcggcttcg
     1021 gtgttggtga ttgatttgag ctgcctggca acttcaagat gcctcttaat tcgttcatag
     1081 tcaagtcggt tcaatgttat cctgctcatg gtttcatgat tttagtataa tttgaaacgt
     1141 gataaagata ggcaatgttt acgtgtaaat gaaatcttga ggggaggtgt tttaggtggc
     1201 acgctagctt cagctggatg atttttctgt accattctga gctactttac ccataaccta
     1261 catagggtta gcaggttata atctggcttt gcaaaaacct gttgttaaac tagcgattcc
     1321 gccctgtggg ctggtgggcg aggaaggttt tgatcaggtg atccgggcgg gattagctcg
     1381 cctgcggctc gtgatcccgc cctgccgtaa cgcaaaaagt aaaggcaatt caagttcaga
     1441 attgccttgg atacgcgcag tgtttctcag gtgatccggg cgggattagc tcgcctgcgg
     1501 ctcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccctgc ctgaattgag
     1621 gaggggagtg gcttccccat cgtgatccgg gcgggattcg aacccgcgac ccacagctta
     1681 gaaggctgtt gctctatcca gctgagctac cagaccttaa cttttacggg tgcaaattta
     1741 gacaatttat cgaaaaaaca ggtacaaccg ctcaactagc tataaatatt aaccagacac
     1801 gctgcaggat gtagtatagg tgcttaacag atttcttctc ctgggcatgg tctgagcctt
     1861 tactttactg gctatttatt ctaaatggtc tatttgactt tcagggattt ctttaagctg
     1921 aaatttcatg ttgaaatatt taccagagta tggatatggg tcgttttcat ctattctatg
     1981 ttctcaacca ggttaatttg gactacagac aattttgagt aaagtttata gcatttgcca
     2041 aacgtttgtt ttatgcgccg tgcgggatta cgatgttgcg aacctatgtg ccgtattaaa
     2101 cttcgaatca cactgtttgc aaattaaact acctgctgcg attattgcat ctgttgactg
     2161 ctgttttgat tattttgctt gtcatacagc ctaaaaaaag tcaaattacc cctatctcaa
     2221 gctgtttact aagtttatct aaattgaatt ttgcggaatt taaattacct gatttttgaa
     2281 ttggcataat ttttttccca ttagaatcat aaagaatcaa tctatagtca ttatcggcta
     2341 tatcaagagt tcgattactc tggctgtatg ttcgccacaa tttagtagat ttttttatgc
     2401 caattttcat gtcggattga ataagaatcc attttccaat tggaaataac ccgaaaatgt
     2461 aaattgaaaa tctgagtctt tttttcccaa aatctatcga ggtaattgtt gacgtaaatc
     2521 caacgaaagc acctatcaga actacaacta atccagctat tgaaaaatag gttataattg
     2581 agccagcaac aaacagaaat atacctgcca gggttccaac cggaccaaac attttgtcaa
     2641 ttttattttt tgttgtcatt ttcaatctat tgtaagatat tagcaggttc tacaaattgc
     2701 taccaacagt ggtggtattg ccagtaaggt attgtggtgc gttttcctat ccagctacac
     2761 aactgattaa gcgggcttaa aactttcgta aaaccactta aacccttaat ggcaattaag
     2821 cgtgttgtag gctgggtgtt tttcaattca atcctgtcaa atttaattgt ttaatccgtt
     2881 tcactcgctc tctttgataa tccattatat cagagtatag tttatctgac cttaaaaaaa
     2941 tcaaatgggt caatgcacaa gccattttat aaccatcagc tatttcaacg gtgcacatta
     3001 tcccagaagt atctccactg ttataaacac ccgtaatttt caattctgtt tttaatgtta
     3061 ttgggtgtcc atttttacga aaaatctgta ccaactctcg aatagggaat gcttttagag
     3121 gcagactatc tgtaatcctt gctgtcaaat cattatattg ttctgaaata tccataattt
     3181 atcagtttac caaatatcaa atttacgttg ttcaattggt atctttctca ctttctcgat
     3241 gtttgtcaat attttatcaa gtaccttttt aaagtcttgt tttctcggtg tgtcgtcaaa
     3301 aaaaatatcc attattactt cttctaaatc ccaatctgtc agccattctt taatcattaa
     3361 ttctgattca tcaagaaatt cgttgtctgt gttgcaatct ccagctaata ctccaaattg
     3421 caaagttaaa tcatgatgga atctttctgc cgtatcaata atcgcagtat gtgtttctcg
     3481 tgataaatca ttcgtgtcca tatgctgtgt cgttttttct gccttgccaa taacggttgg
     3541 cgatttctta cgctggggag ttaaaagcca gcggcgtcat cccacgttaa aactagacta
     3601 aaatactaaa aaatttcgta tttgcatcat caaccccagt aggctaaacc acatgttatg
     3661 ccctgtattt tattgtcagg caactccatt tacggtgaat agttcaatct tttctgtact
     3721 aatgttaatg ctaatttgcc aataatagct acatccgtca agtacaaagt aataccccgt
     3781 ttcgtattta tctctttggt cttcatatcc tagaacttta tgccaaaaag gaaatctatt
     3841 ccaaagcata tttatgtaaa taatcttttc atttttagtg ttaagttctc cagaatattg
     3901 gcgaaagtat ttttttaggt ttttatgaat aattggacaa ttatctccct gattcatcat
     3961 gttaggagta tttgtttctt tgattttatt tttaaggatt atttctgctt tcaaaatgtc
     4021 ttcttcattg ggagtaaatc tcgttccatt aaattcatct ggaaacaagt agacataatc
     4081 tttttcgaga acaataccac aagtgctttt gtattttgtt ttcgaaatgt tttcaatatt
     4141 tgatttttta atctttactg ctgtcgagca agacagtaag attggtatta tcatgtaaaa
     4201 tagtcgcatc ttcataatat ggggcataac ggctgcgtgt ttgttttgtt tgggggatta
     4261 gaacgatgct ctagccaccc gatataaagc taccaaaaag cgacaaactt cccaaactca
     4321 tctcagccca aataaaataa acacgctgtt ggcagaggtt tctacttttt cactaatatg
     4381 taacttcgag gttcaagcaa tattccattt ttagatagta cgaaataggc agctctggta
     4441 ccttctaagt ttgtaaagtc gtatgttagt tttcccgaac tgttttgaga ttttttttga
     4501 gcatttgaaa gttggtaact tccggtaagg gt
//
LOCUS       DAOOQX010000070         4497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_22977_length_4497_cov_2.661414, whole genome shotgun sequence.
ACCESSION   DAOOQX010000070 DAOOQX010000000
VERSION     DAOOQX010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22977_length_4497_cov_2.661414"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2229)
                     /locus_tag="PLX53_01870"
     CDS             complement(<1..2229)
                     /locus_tag="PLX53_01870"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOY71858.1"
                     /translation="MPLQDTVPATDTIKPAAGKSVLEDPVISNAEDSITYSIDGQTAY
                     LYGNAVVTYQNLELKAKYIEFNMATKQVYACGLPDSTGKVVGRPVFKEGGQTYEMDEI
                     HYNFDTKRAKITGVVTEQSGGFMHSKVTKMLEDQVVNLQDGKYTTCDLDHPHFYIAIT
                     KGKMIPDKKIIAGPAYLVIEDVPLPIGIPFGFFPNTRKRQAGVILPEYGEENIRGIFL
                     RGGGVYFGMGDYMDEKLTGDYYSKGSWGLRSQTTYKVRYRFSGTISLEASSYVTSERG
                     LPDYSKDNTYWIRWNHTQDAKANPNSTFQANVNFGSSNHNRYNARNINNALANTFNSS
                     ISYSRIWPGSPFSMSASLNHSQNSIDKSVDLGFPKVSLNMSRIYPFKRKLSVGKPSWY
                     EKIGLSLNTNLENRVQVKEDNLFKSSVVDSMQNGVRHDIPISTSFNLLKFITISPSAS
                     YNEFWYLQTINKHWDPDSQVVVVDTIKGFKRGYQYSTAVSMSTKIYGTFQFGKKSKVQ
                     AIRHVITPGISLSYRPDFAKESFGFYKSVQVDSTGRMAQYSIFERGIYGGPGGGKSGV
                     VNFSLGNMVEMKVLSPKDTANPVKKVKILEGFSINTSYNMLADSLNWSDISVSGRTNL
                     FNNININFSGAFSPYAIDAKGSRYNRFELDRSGKLARFTRGSVGLDFALNSSKGSASS
                     GGDQSRSQGMGGSNPPLGTAPDGSSFGESLGTQYGMDYVDFSVPWNLRVSYNLSYSKP
                     AYV"
     gene            2387..3565
                     /locus_tag="PLX53_01875"
     CDS             2387..3565
                     /locus_tag="PLX53_01875"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOY71859.1"
                     /translation="MEGLRLKHFLPIPLLVVLSLLLAVGEKALSQTSSSYQLTRVVVD
                     AGHGGKDPGTVGRVSKEKDIALSIALKVGSYIEKNFPEVKVIYTRNDDTFIPLDERSR
                     IANEAKADLFISIHCNSAPNRTSFGTETYVMGLHRSADNLDVAMRENAVISYEDDYST
                     RYEGYDPSSSESYIIFSMLQNAFLDQSLSLANLIQTDFRDRASRKDRGVKQAGFLVLW
                     KTSMPSVLVEVGYLSNASEEKYLNSQEGQDYIASSIYRAFKEYKKNVDSYSNQLAQIQ
                     LTKPADSSVEQKGNTQVQPPSSTSQSHDFPIFKVQLMASNKPIPLDSPNFQNIKTVEE
                     LIINDSYKYCVGSLSSYDEALKLCREVRKQFPDAFVIATKNGVPVALQQAIKDNKTKN
                     "
     gene            3767..>4497
                     /locus_tag="PLX53_01880"
     CDS             3767..>4497
                     /locus_tag="PLX53_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOY71860.1"
                     /translation="MKIKLTKEAKIGIFMVACLFAMFWGINFLKGKNVFSSNNTYYVV
                     FTSVDGLKPTNDVLINGFKVGLIEEIEFNDIKSGKFKVNLLISKKYKLPRNTIAKLIS
                     ADLMGGKAIKLEISSDTAYYQPGDTLPGVIEVGLIDQLGHQMTPVKEKAEQLMVQLNN
                     TLLVVEKVFNEENRDQLSQGIANLNASLNNISQISAALDTMLNSPKGSLKTTFSNIES
                     VSSNLKKSNKELTNIIQNVSSVSDS"
BASE COUNT         1257 a         1074 c         1003 g         1163 t
ORIGIN      
        1 tacataggca ggtttggagt agctcaggtt gtagcttacc ctgaggttcc atggaacgct
       61 aaaatcgaca taatccatgc cgtactgtgt gcccagagac tcaccaaacg acgaaccatc
      121 gggtgcggtt cctaaggggg gattgcttcc acccattccc tgcgacctgg actgatcccc
      181 acctgaagag gcgctaccct tgctgctatt caacgcaaag tctagcccaa cgctgcccct
      241 ggtaaaacgc gccagcttgc cgctcctatc aagttcaaac ctgttgtacc tgcttccctt
      301 agcatcaatt gcatagggac taaatgcacc ggaaaagtta atgttgatgt tgttgaaaag
      361 attggtgcgg ccgctaacgg agatatccga ccagttcaga gagtcggcca gcatgttata
      421 ggaggtattg atgctgaacc cttccagaat ttttaccttt ttaaccgggt tagcagtatc
      481 ctttggcgag agcaccttca tttcgaccat gttacctaaa gaaaagttca cgaccccaga
      541 ttttccacct cccggcccac cgtaaattcc cctttcaaaa atggagtact gtgccatcct
      601 tccggttgaa tccacctgaa cagacttgta gaatccaaaa ctctccttgg caaagtcggg
      661 acggtagctc agcgaaatac cgggtgtaat cacatgcctg attgcctgca cctttgattt
      721 ctttccgaac tggaatgtcc cataaatctt tgtactcatg gacacggcgg tgctgtactg
      781 gtatccccgc ttgaagcctt ttatggtatc aaccacaact acctgtgaat cgggatccca
      841 gtgcttgttg attgtttgca agtaccagaa ctcgttgtag ctagcacttg ggctaatggt
      901 tataaacttt agcaggttaa atgaggttga aataggtata tcgtgcctca cgccgttttg
      961 catgctgtca accaccgaac ttttaaacag gttgtcctcc ttcacctgca ccctgttttc
     1021 aaggtttgtg tttaggctca acccaatctt ttcgtaccac gagggtttcc caacgctcaa
     1081 tttacgcttg aatgggtaaa tacggctcat gttgagcgaa actttcggaa aacctagatc
     1141 aacagacttg tctatgctat tttgcgaatg gttaagcgaa gccgacatgc tgaagggtga
     1201 acccggccat attcttgagt acgaaatgct ggagttgaaa gtgtttgcca gggcattatt
     1261 tatgtttcgg gcattgtacc ggttatggtt ggatgagcca aagttgacgt tggcctgaaa
     1321 ggtggagttg gggtttgcct tagcatcctg tgtatggttc caccttatcc agtaggtgtt
     1381 atccttacta tagtccggca agccacgttc actggttaca tatgatgatg cttctaggct
     1441 tatagtgccg gaaaaccggt agcgaacctt gtaggtggtt tggctgcgta acccccatga
     1501 gccctttgag tagtaatcgc ccgtaagctt ctcgtccatg tagtcgccca tgccaaagta
     1561 aacaccccca cccctcagga aaattcccct gatattttcc tcgccatact ctgggagaat
     1621 tacccctgct tgccgttttc gggtgttggg aaagaaccca aagggaatgc caattggtag
     1681 cggtacatcc tcgataacca ggtatgcagg cccggcaata atttttttat cgggaatcat
     1741 ctttcccttg gttatggcta tgtaaaaatg tggatgatcc aaatcacagg tggtgtactt
     1801 accatcctgc aggtttacta cctggtcttc aagcatcttg gttaccttgc tgtgcatgaa
     1861 accaccagac tgctcggtaa ccacgccagt aatcttagcc cgcttagtat cgaagttata
     1921 gtgtatttca tccatctcgt aggtttggcc accctcttta aacaccggac gaccaaccac
     1981 tttccccgtg ctatcgggca aaccgcaggc gtaaacctgc ttggttgcca tgttaaactc
     2041 gatgtacttt gcctttagct ccagattttg gtaggttacc acagcgttgc catagaggta
     2101 ggccgtttgc ccgtcaatag agtaagtaat agaatcctca gcattactta tcacagggtc
     2161 ttccagcacg cttttgccag ctgcaggttt aattgtatcg gtagcgggca ccgtatcctg
     2221 aagtggcacc tgcaatacaa aattgggcga aaaaggtatg gcctgcctag caaaccctaa
     2281 tgagctatat aaaacaataa aaagcgttgt taccctgaat ttcaatttta gtcgtatttt
     2341 tgtaacaaaa ttgagctcaa aactactaaa aaatttttag ggaataatgg aggggctgcg
     2401 cttaaaacat tttttaccaa tacccttgct ggtggttcta tccctcttgc tggcggttgg
     2461 agaaaaagct ttgagccaaa ccagcagctc ctatcagctc accagggtgg tagttgatgc
     2521 ggggcatgga ggtaaggacc caggaacagt tgggcgggta agcaaggaaa aggacattgc
     2581 ccttagcatt gctctaaagg taggaagcta cattgaaaag aacttccctg aggtgaaggt
     2641 aatctacacc agaaacgacg acacctttat accgttagac gagaggagta gaattgccaa
     2701 tgaagcaaaa gccgacctct ttatctccat tcattgtaat tccgccccaa acagaacttc
     2761 ttttggcacc gaaacatacg tgatggggtt gcaccgatcg gccgacaacc tggacgttgc
     2821 catgcgtgaa aacgctgtaa tttcgtatga agatgactac tcgacaaggt atgagggtta
     2881 cgacccaagt tcttccgagt cgtacataat cttctccatg cttcagaatg cctttcttga
     2941 tcaaagcctc tcgcttgcca acctgattca aactgacttt agagacaggg ctagccgaaa
     3001 ggacagaggg gtaaagcagg ccgggttcct tgtgctatgg aaaacctcta tgccaagcgt
     3061 cctggttgaa gtgggttacc tcagcaacgc cagcgaagag aagtacctga atagccagga
     3121 gggtcaggac tacattgcct cctcaattta ccgagccttt aaggaataca agaaaaacgt
     3181 ggatagctac agcaaccagc tggcacaaat acagctcact aagccagccg acagcagcgt
     3241 tgaacagaaa ggcaacaccc aggttcaacc cccatccagc acaagccaaa gccatgattt
     3301 tccaattttt aaggtgcaac taatggcttc aaacaagcca atccccctcg attcacctaa
     3361 ttttcagaat attaaaacag ttgaagagct tatcataaac gattcctata agtactgcgt
     3421 aggcagcctg agctcctacg acgaagccct taaactctgt agggaagtgc gtaagcaatt
     3481 tcccgatgca tttgtaattg ccacaaaaaa tggagttcct gtagcgctac agcaagccat
     3541 taaggataac aaaaccaaaa attgataggt ggaaatttta cttaccctta ttcaggtata
     3601 aactgactac acgtacagaa ccagctaaca ctaatcacca ttacaaacaa tagcaataaa
     3661 tattttaaat agccgcgggc aacgtgaaga gaaaattacc ccagattgat gtggttgtgt
     3721 atctttgcca tctgccagct agttaaatgt taatactact ttagaaatga aaataaaact
     3781 taccaaagaa gcaaaaatag ggatattcat ggtggcctgc ttgtttgcca tgttttgggg
     3841 tatcaacttt ttaaagggga aaaacgtgtt cagctccaac aatacatact atgtagtttt
     3901 tacatcggtt gatgggctaa aacccaccaa tgatgttctc atcaacgggt ttaaggttgg
     3961 cctgatagaa gaaattgagt tcaacgacat caaatcgggg aagtttaagg ttaacctttt
     4021 gattagcaag aagtacaagt tgcctcgaaa caccattgcc aagctaatca gcgccgattt
     4081 aatgggcggt aaggctatta agctggaaat ttcgtccgac acggcctact accaacccgg
     4141 cgatacccta cctggagtca ttgaggtagg cctgattgac cagctgggcc accagatgac
     4201 cccagttaaa gaaaaagctg agcagctcat ggttcagctg aacaatacgc tgctggtagt
     4261 tgaaaaagtt ttcaacgagg agaatcgcga ccagctatca cagggtatag ctaacctgaa
     4321 tgccagcctc aacaacatta gccagataag cgcggcactc gacaccatgc tgaactcccc
     4381 taagggctcg ctcaaaacca cctttagcaa catcgaatcg gttagcagta acctcaagaa
     4441 aagcaacaag gagcttacca acattattca aaacgtgtcg tcggtgagcg actcatt
//
LOCUS       DAOOQX010000071         4489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_23020_length_4489_cov_3.751691, whole genome shotgun sequence.
ACCESSION   DAOOQX010000071 DAOOQX010000000
VERSION     DAOOQX010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23020_length_4489_cov_3.751691"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLX53_01885"
     CDS             <1..212
                     /locus_tag="PLX53_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOY71861.1"
                     /translation="EAINSNEVNLLVCGHSHILLVKFDSKHNLLFINPGAAGNNGFHK
                     IKTVVKLDIEGKQMKNLQVWEKTRF"
     gene            complement(407..1678)
                     /locus_tag="PLX53_01890"
     CDS             complement(407..1678)
                     /locus_tag="PLX53_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOY71862.1"
                     /translation="MGTTIIIILISLFFSFFFAGMEIAYLSANKLKLELDKKKGGFTS
                     HVIAVLAGNKEQYIATMLVGNNIALVIYGIEIAVLLTPALGKLIQIPSLLLLVQTLIS
                     TIIVLVTAEFLPKSIFRLHPNAFLRFFALPVYILYIIFYPISKFTSWLSYWIIRLVFR
                     IKVKHDGSKPIFNKVDLDFLVDQASSADDGEGDHTQELKIFQNALDFSEVRLRDCMIP
                     RTDIEAIDVNATVDELRAKFAETNYSRLPVYSNSIDNIVGYVNSKVLFINPQSIKSKL
                     INIDFVPETMPANKMLTRFIKEHKSIAIVVDEFGGTAGLVTIEDIMEEIFGEIDDEHD
                     RNEFVEKKLSDNEYIFSGRLEVKHLNENYQLDIPESDDYETLAGFILFKHQNIPKLNE
                     TIEFEGFIIKILRVNETRIELVRLTRIKGKG"
     gene            complement(2214..2780)
                     /gene="lptC"
                     /locus_tag="PLX53_01895"
     CDS             complement(2214..2780)
                     /gene="lptC"
                     /locus_tag="PLX53_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOY71863.1"
                     /translation="MRITLFKGYKYILPGIAIVGTTFFFGCKTDTEAIQYFSDKKSIP
                     GVTAYNSELEYTENGVVKIMVKAPITIYYQFSEEPYTEFPEGITVYTYDDTLGVESNL
                     TAKYAIYYDKKMLWHAKNNVVARNRKGEVLNTEELYWDQGKHLIYTDVNVKINSVNGN
                     IYGKGLVSDEKFDSWEVKEPYDGTILLE"
     gene            complement(2799..4067)
                     /locus_tag="PLX53_01900"
     CDS             complement(2799..4067)
                     /locus_tag="PLX53_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71864.1"
                     /translation="MGFTLAALEVFSQNIYTYSPYSRFGIGIIPTHGFSQTRAMGGVS
                     QSVRSPYMINYLNPASYAAQDSMSFTFDFGLESNNSSFSGYDQYLNKVSASSSAGGIH
                     HIAIAFPVSKKIGMAAGIAPYSSVGYRLIRFETDPYILSTIGRIKYEHAGKGGINQLF
                     VGAGYAPFKNFSIGANFIYYFGSLDYNNNIIFPSSSYSYTNASVGSSIMVSDVSFNVG
                     FQYRLFLDEDKNNSLGFGVTVDAGSDINMKYKWLTKLEGSTYVDSLIPHPQSKSKFKL
                     PTGVNSGISYTFKDKLFATAEYYYQDWTKTEAFNTTLQMGKMEAFRLGVEFTPNRSDL
                     KSYLKKISYRAGGFYNKTNLIINSRQIEEYGISLGFGLPFRNNTSFNLSAELGRRGTN
                     ENYLIKESFFVLNLGITFHDSPWFFKRKYY"
     gene            complement(4087..>4489)
                     /locus_tag="PLX53_01905"
     CDS             complement(4087..>4489)
                     /locus_tag="PLX53_01905"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOY71865.1"
                     /translation="NQNVLVVDCGTAITYDVVTSDEIYLGGGISPGISLRFKSLNDYT
                     AKLPLLSISNTFSLIGRNTNDCILSGVLNGVVAEVDAYIEKFNLIYSNSRVVLTGGDS
                     SFFVDKLKNSIFVNPNLIYTGLNRILELNAE"
BASE COUNT         1295 a          941 c          968 g         1285 t
ORIGIN      
        1 atgaagccat taatagcaat gaggtcaact tgctggtttg tgggcatagc catatactcc
       61 tggtaaagtt tgatagcaag cataacttgt tgtttattaa cccgggcgca gccggcaaca
      121 atggatttca caagattaaa acggtagtta agctggatat tgaaggtaaa cagatgaaaa
      181 acctgcaggt ttgggaaaaa acacgctttt agtccagcag ttgtcctaaa ttagccagcg
      241 ccagatcttc ttgaatttgt caagtagcgg ataagttttg aaatcctcca ggaggcggac
      301 tagttctgaa atctttaatc gccggtaggc ggacaagttc ttgaatctgg aatctgaaat
      361 ctggaatctg aaatccgccg gtaggcgggc aatttcttga actatcttac cctttaccct
      421 ttatccttgt caacctcacc agctcaatgc gggtttcgtt taccctgagg attttaatga
      481 taaaaccctc aaactcgatg gtttcgttta gtttgggtat gttctggtgc ttaaacagga
      541 taaagccggc aagtgtttcg taatcgtcgg actcaggaat atcaagctgg tagttttcgt
      601 tgagatgttt cacctcaagc cgtcctgaaa aaatatactc gttgtcgcta agcttcttct
      661 cgacaaactc gttgcggtcg tgctcatcat caatttcacc gaatatttcc tccatgatat
      721 cctcaatagt aaccaggcca gctgttccgc caaattcatc aacaacaatg gcaatacttt
      781 tatgctcctt tataaaacga gttagcattt tattggccgg catggtttcg gggacaaagt
      841 cgatgttgat gagctttgat tttatgcttt ggggattaat gaagagaact ttagaattaa
      901 cgtagccgac aatattatca attgagttgc tgtaaacggg taaccgggag tagttggttt
      961 cggcaaactt ggccctgagc tcatcaacag tggcattcac atcaatggct tcaatatcgg
     1021 ttctgggtat catacaatcg cggagacgta cttcggaaaa atcgagtgcg ttttgaaata
     1081 ttttcagctc ctgggtgtgg tctccttccc catcgtcggc cgatgaggcc tggtcaacca
     1141 gaaaatcgag gtcaaccttg ttaaagatag gcttagagcc atcgtgcttt acctttatcc
     1201 taaataccaa cctaataatc cagtaggaaa gccaggaagt gaacttgctg atggggtaaa
     1261 aaataatgta caggatgtaa acaggcaaag caaaaaacct aaggaaggcg ttagggtgca
     1321 acctaaaaat tgacttgggt agaaactctg cggtaaccaa aactattatg gtgcttatca
     1381 gggtttgcac caggagtaaa agggaaggaa tctgaatcaa cttacccaat gcaggggtaa
     1441 gcagaacggc gatttcaata ccgtagataa ccagcgcaat gttgttgcca accagcatgg
     1501 tggcaatgta ctgttcctta ttacctgcta aaaccgctat aacgtgcgat gtaaatccac
     1561 cttttttttt atcgagctcc aacttaagct tattggccga aaggtaggct atttccattc
     1621 cagcaaaaaa gaatgagaaa aaaagtgaaa tgagtatgat gattattgtg gtacccatgt
     1681 tttacctgtt tactcttccg gcttttccct tcgtcttcgt cttataaaat acataaggcc
     1741 ggctagtagg gctagtaaaa aaagtatata gctggaggca aaaccgtttc gaacggtttc
     1801 ataaatcccc agcgcaacta ggaaaacagt gagcagtagc caaatcattt caagtatgta
     1861 ggcaaatcct ttcacagcta aagagataat tcaccaactt agtttctagg atgctatgta
     1921 tttttttctg cataaagtta caaatttaag cttaaaaagc attgcccgat gttggacaaa
     1981 attacccatc gcctttctag tctaaactga atgataaatt gttccagtat gcctccttga
     2041 aaaatatggc agccgaatgt ttagttttcc gagttctact gggtaattcc cgggaaaaaa
     2101 attacatagg tacaacaggc aacttgatac tggcattgac gaggataaac aatgctgggt
     2161 gaaagaatat ttgaggagaa atcctaataa ccatggggta atccagctat ctgctattca
     2221 agcagaatag ttccatcata gggttcttta acctcccagc tgtcaaactt ttcgtcggat
     2281 acaagcccct tcccgtaaat gttgccattt acgctgttaa tttttacatt cacatccgta
     2341 taaatcagat gtttgccttg gtcccagtag agctcctcgg tgttaagcac ctcccctttc
     2401 cggtttcgtg ccacaacgtt attctttgca tgccaaagca tttttttatc gtagtaaatg
     2461 gcgtatttag cagtcagatt cgactcaaca cccagggtat catcataggt atatacggta
     2521 ataccctcgg gaaattcggt gtagggttct tcggaaaact ggtagtagat ggtaataggg
     2581 gccttcacca taattttaac caccccattt tcggtgtact caagttccga gttgtaggcg
     2641 gtaacgccag gtatgctttt cttatccgaa aagtactgga tggcttcagt atcggtcttg
     2701 cagccgaaaa agaaagtagt acccactatg gcaatgcctg gcagtatgta cttgtaaccc
     2761 ttaaatagtg tgatgcgcat agctaaagag tttacctgct agtagtactt acgcttaaag
     2821 aaccatggtg aatcgtgaaa ggtaatgccc aggttcagga caaagaacga ctccttaata
     2881 agatagtttt catttgttcc ccgcctacca agttcggccg acaggttaaa gctggtatta
     2941 ttcctaaagg gtaagccaaa cccaagggaa attccatatt cttcaatctg cctagagttg
     3001 ataataaggt tagtcttatt gtaaaatccc cctgcccgat agctgatttt tttaaggtag
     3061 ctcttcaaat cgctacggtt aggggtgaac tcaaccccca aacggaaggc ttccattttt
     3121 cccatctgaa gggtggtgtt aaaagcttcc gttttagtcc aatcctgata gtaatattcg
     3181 gctgttgcaa agagcttatc cttaaacgtg taggaaatac ctgagttaac acctgtagga
     3241 agtttgaatt tacttttaga ttggggatgt ggtatcaggg aatcaacgta ggttgaccct
     3301 tcgagcttgg ttagccactt atacttcatg ttgatatccg aacccgcatc cacggtaacg
     3361 ccaaacccca acgagttgtt tttatcctcg tctaggaaaa gcctgtactg gaaccccacg
     3421 ttaaacgata catcgctgac cataatgctt gagcccacac tggcattagt gtaggaatag
     3481 gacgaggacg gaaagattat gttgttgttg tagtcgaggc ttccaaagta gtagatgaaa
     3541 ttagcgccaa ttgagaagtt tttaaaagga gcgtaacctg caccaacaaa cagctggtta
     3601 atgcctcctt tgccggcatg ctcgtactta attcttccaa tagtgctaag aatatatgga
     3661 tcggtttcaa aacgaattag cctgtagccc acgctgctat atggggcaat gccagcagcc
     3721 attccaattt ttttcgacac cggaaaagca atggcaatat ggtgaatgcc ccctgcgctg
     3781 cttgaggccg ataccttatt caggtactgg tcataaccgc tgaacgatga attgttgctc
     3841 tccaaaccaa aatcaaacgt gaacgacatg gaatcctgag cagcataaga ggctgggtta
     3901 aggtagttta tcatgtaggg gcttcttacc gattgcgaaa cgcctcccat tgccctggtt
     3961 tggctaaacc catgagttgg tataatgcca attccaaacc tggagtaggg cgaataggta
     4021 tagatatttt gtgaaaaaac ttctagagct gctagcgtaa agcccactaa aagtaaaatt
     4081 tttctactat tctgcattga gttccaaaat tcggtttaac ccggtataga ttaaatttgg
     4141 gtttacaaat atgctatttt ttaacttatc tacaaagaag ctggaatccc cacctgttaa
     4201 aactaccctg ctgttgctat aaattaggtt aaatttctcg atatacgcgt caacctcagc
     4261 aaccacgcca tttagcacac cggaaagtat acagtcgttt gtgttccgac caatcaggct
     4321 gaaggtattg gaaatgctta gtaggggtag ctttgcggtg tagtcattca acgatttgaa
     4381 gcgtagcgat attccgggtg aaataccacc acccaggtag atttcgtcgc tagtcaccac
     4441 atcgtaggta atggccgttc cacagtcaac aaccagcacg ttttgattt
//
LOCUS       DAOOQX010000072         4466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_23172_length_4466_cov_3.283382, whole genome shotgun sequence.
ACCESSION   DAOOQX010000072 DAOOQX010000000
VERSION     DAOOQX010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23172_length_4466_cov_3.283382"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PLX53_01910"
     CDS             <1..556
                     /locus_tag="PLX53_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HOY71866.1"
                     /translation="TGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGKDSSKV
                     DRSGAYAARHIAKNMVAAGIADEVLIQVAYAIGVARPINLYVNTYGTAKVSLSDGEIA
                     QKILTIFDLRPAAIVKRLGLKNPIFEETAAYGHFGRDHYIRNVDFIENSNCNGEKIVK
                     REVEFFTWEKLDYVDRLKSEFGIK"
     gene            617..1540
                     /locus_tag="PLX53_01915"
     CDS             617..1540
                     /locus_tag="PLX53_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY71867.1"
                     /translation="MSIGISHITKQFGEQKALNDVSFNISTGQVVGLLGPNGAGKSTL
                     MRIITGYLFQDSGSVDINGHNTQDTRFDFRREIGYLPENNPLYTDMYVKEYLSWVASI
                     YKLGKEKRRRLSELIGITGIGDEQGKKIGSLSKGYRQRVGLAQALIHNPSVLVLDEPT
                     SGLDPNQIVEIRNVIEEIGRDKTVIFSTHILQEVEAICSRIIIINKGLVVADGTNTEV
                     KALSSGLRQNIVVEFAEKVSREAIMAIEGVETATLIDTNCWWITSGRLEDLRGKIFNF
                     AKNAGFTLLTLQKRDLQLEEVFQNLTLGNRN"
     gene            1580..2467
                     /locus_tag="PLX53_01920"
     CDS             1580..2467
                     /locus_tag="PLX53_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71868.1"
                     /translation="MENNEKSAKTIKTLRVIMVMLLLIIAGLTVVYFQQKQQAKDTQE
                     ILQAQKDSISNNLLRLMNDYSELETTNDTLNQKLQKEQEHAKKLYAELQSVKQISYAK
                     IKEYQKELGTLRSIMKDMLHDIDSLNALNKELIAENVKVKEEAATAKQTVIELEQKTE
                     ELNTQVAKGSIVKARDIVAMGVNQKGNEVTRARRVNKIRACFTLSENSIAKPGTRNVY
                     MRIVGPDDFVLTKSEADLFDFEGEKIVFSASREVDYQNSDLDVCIFFDNNGELLTGVY
                     KVTLYMDGYMIGYSEFSLK"
     gene            2715..4298
                     /locus_tag="PLX53_01925"
     CDS             2715..4298
                     /locus_tag="PLX53_01925"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOY71869.1"
                     /translation="MEKLTKIAACLAGLFILIAVATGCHEREQAKVGETEYLEEITIK
                     QLQQGYREGKFTVAQVVNDYLTRIEELDKNGPVLNSIIAINPEAMRIAQELDSELAAG
                     KSRGPLHGIPVILKDNIESKDSMPTTAGAVVLKNSYVWRDSHVAKKLREAGAVIIAKA
                     NLSEWANFRASLSSSGWSGVGGQTRNPYDTERNPCGSSSGSGVAVAANLCAFAIGTET
                     NGSIVCPSNNNGIVGLKPTVGLISRTGIIPISFTQDTPGPMARTVEDVAVCLGIMVGV
                     DSTDAKTLNPDAVFYSDYSKFLKRDGLKGKRIGLLTNVSGFHFKVDTLMKQSVDAMKQ
                     AGAEVVAIDFPVSREVDNASFEVLLYEFKDGLNRYFASLGESARVKNISELIKFNLND
                     SVELMYFDQQLLEMAQSKGDLNSPEYKKNLEMMQTGMRKQGIDRIMNEYKLDALVAPT
                     GSPAWKTDLVDGDHFLGGSSSYAAIAGYPSISVPMGFIDGLPLNISFIGKAWSEAELL
                     SIAYSYEQATHQRKKPMLE"
BASE COUNT         1396 a          869 c         1087 g         1114 t
ORIGIN      
        1 aacgggcaag tttgttattg gcgggcctca tggcgatacc gggctaaccg gcagaaaaat
       61 cattgtagat acttacggtg gaaaaggagc gcatggcggt ggagcttttt caggaaaaga
      121 ttcttcaaaa gttgacaggt cgggagctta tgccgctcgt cacattgcga agaatatggt
      181 ggcagctggt attgccgacg aagtgctaat acaggtagcg tacgctatcg gagtagccag
      241 gcctatcaat ctatatgtta atacatatgg cacagctaaa gtaagcctca gtgatggtga
      301 aattgcccaa aaaatattga ccattttcga tctgcgacca gcagcaatag ttaaacggct
      361 gggacttaaa aatcccatat ttgaagaaac tgccgcctac gggcattttg ggcgcgacca
      421 ttatatacgt aatgttgatt ttattgagaa tagcaactgc aacggagaaa aaattgttaa
      481 gcgggaagtg gaatttttta cgtgggaaaa gctcgactat gttgataggc ttaaaagtga
      541 gtttggaata aaataaaccc attcccaatt ccgtttattt gaacgaagaa ctagcattta
      601 cgggtttagt ataacgatgt caataggaat aagccacata accaagcagt ttggagagca
      661 gaaagcgctc aacgatgttt cgttcaatat ttcaacaggt caggtggttg gacttttagg
      721 gccgaacggc gccggaaagt caacgttgat gagaattatc acgggctacc ttttccaaga
      781 cagcggaagt gttgatatta atggtcataa cacgcaggat acccgatttg attttagaag
      841 ggagattggg tatttgcccg aaaataatcc tctttatacg gatatgtacg tgaaggaata
      901 cctatcgtgg gtggcctcca tctacaagtt gggaaaggaa aagcgccggc gcttatcgga
      961 gcttattggc attacgggta taggcgatga gcagggtaaa aagatcggca gcctctccaa
     1021 ggggtacagg caacgggtag gcttggcgca agcattaatt cacaacccct ccgtgctggt
     1081 gctcgatgag ccaaccagcg gtttggatcc taatcagatt gtcgaaattc gtaacgttat
     1141 tgaagaaata ggcagagaca agactgttat tttctcaacc catatcctac aagaagttga
     1201 agcaatctgc agcagaatca tcatcattaa caagggttta gtagttgccg atggaaccaa
     1261 caccgaagtt aaagctttgt cgtcggggct taggcaaaac atagttgtag agtttgccga
     1321 aaaggttagt cgtgaagcta tcatggctat tgaaggggtg gaaacggcaa ccctcattga
     1381 tactaactgc tggtggatta cctctggcag gctcgaagac ttacgaggca aaatatttaa
     1441 ttttgccaaa aatgcaggat tcacgctact tacccttcaa aaaagagacc ttcaacttga
     1501 ggaagtattt caaaatctta cccttggtaa cagaaactag cactaaacct aacagtttta
     1561 actaaataat aaaagacaaa tggagaataa tgaaaaatca gccaaaacca ttaaaactct
     1621 aagagtaatt atggttatgc ttctgttgat tatagctgga ttaactgttg tgtacttcca
     1681 gcaaaagcag caagcaaaag atacacagga aatactccag gcacaaaagg actcaatttc
     1741 taataatttg ctgaggttga tgaatgacta cagcgagctg gagactacca acgacacgct
     1801 gaatcaaaaa cttcaaaagg agcaggaaca tgccaaaaag ttgtatgccg agcttcagag
     1861 tgtgaagcag attagctatg ccaaaattaa ggagtaccaa aaggagctag gcaccctacg
     1921 ttcaattatg aaggatatgc tccatgatat agattccttg aatgctttga acaaggagtt
     1981 aattgcagag aatgttaagg tgaaagagga ggcagccacc gccaaacaaa cagtaataga
     2041 gcttgagcaa aaaacggaag agctcaatac ccaggttgca aagggttcaa tagttaaggc
     2101 acgcgatatt gtagccatgg gtgtaaacca gaaaggcaac gaggtaaccc gagcacgcag
     2161 ggtgaataaa attcgtgcat gctttaccct aagcgaaaat tctattgcta aaccgggaac
     2221 ccggaatgtt tacatgcgga ttgttggacc cgatgacttt gtgctgacca agtcggaagc
     2281 cgatttgttc gattttgagg gcgaaaaaat tgttttctcg gctagtaggg aggtcgatta
     2341 ccagaacagc gatcttgatg tgtgcatatt ttttgataac aatggagaac ttttgacggg
     2401 cgtttataag gttacactgt atatggatgg ctatatgatt ggctactctg aattttcttt
     2461 aaagtaaagc gtttaacctg ggataattcg gaaaaggcgg ttcagcaaac cgccttttcc
     2521 tattaccccc accctggata tgtatttgat tttaaaccta gttgagctaa cttggtcaaa
     2581 attaaagcat acttatagca tatgacatag ttatgccata cgaaaaaggg tgccgcagca
     2641 acactcagca cagcattgtt tgtgtaaagt agatatgaaa agcaagtcct aacactatta
     2701 aaaaatttgc aaaaatggaa aagttgacaa aaattgccgc atgtctagcc gggctgttca
     2761 tacttatagc ggtagctacg ggttgtcacg agcgggaaca ggcaaaagta ggggaaaccg
     2821 aataccttga ggaaattact ataaaacagc ttcaacaggg ataccgggag ggaaagttca
     2881 cggtggccca agtggtaaac gattacctga caagaataga ggagctggat aaaaatggtc
     2941 ccgttcttaa ctcaataatt gctattaatc ccgaggccat gagaatagcc caggagctcg
     3001 acagcgagct agccgcaggg aaaagccgtg gtcccttgca tggcataccg gtaatactga
     3061 aggataatat agaatcaaaa gattcaatgc cgactactgc tggtgcagtt gtcttaaaga
     3121 attcatacgt ttggcgggat agccatgttg ccaaaaaact tcgcgaagcc ggggcggtta
     3181 ttattgccaa ggcaaatctt agcgaatggg caaactttag agccagtctc tcatcaagtg
     3241 gttggagcgg agttggtggg caaacccgca atccttacga taccgaaaga aacccatgcg
     3301 ggtcaagttc cggttcaggg gttgccgtag cagccaacct ttgtgctttt gcaattggaa
     3361 ccgaaacaaa cgggtctata gtgtgcccgt ccaataacaa cggcattgtt gggctgaagc
     3421 ccacagtagg cctgattagc cggacgggaa ttataccaat ttcatttacc caggacacac
     3481 caggacccat ggcgcgtaca gttgaggatg tggcagtatg tttaggaatt atggtgggtg
     3541 tcgattctac cgatgcaaaa accctaaacc cggatgcggt attctattct gactattcaa
     3601 aatttttaaa aagagatggg ctgaaaggta aacgaatagg tttgcttacc aacgtatcag
     3661 gatttcattt taaggttgac acccttatga aacaaagtgt tgacgccatg aagcaggctg
     3721 gagccgaagt ggtagctatt gattttccgg taagcaggga agttgataac gcttcgttcg
     3781 aggttctact gtatgagttt aaagatgggt tgaaccgcta ctttgccagc ctgggggaga
     3841 gcgctcgggt aaagaatatt agcgaactga ttaagtttaa cctgaacgat tctgtggagt
     3901 taatgtactt cgaccagcag ctactggaaa tggcacaatc aaaaggagat cttaacagcc
     3961 ccgagtacaa aaaaaatctt gagatgatgc aaactgggat gcgcaagcaa ggaatcgacc
     4021 gtataatgaa cgaatacaag ctggatgcac tagttgcacc tactggttca ccagcctgga
     4081 aaaccgattt ggttgatggc gatcactttt tagggggaag ttcgtcgtat gcagcgattg
     4141 ctggttatcc aagcataagc gttcctatgg gatttataga tggcctcccc ctaaatattt
     4201 cgtttatcgg gaaagcctgg agcgaggcgg aactattaag cattgcctac tcctacgagc
     4261 aagctaccca tcaaaggaaa aaacctatgc tggaataacc accataaaag aggcccgcca
     4321 aacaagcatt agccatttgc gttagctggt tattttcagt caaaagttca tcgtgtgttg
     4381 ctaaattact gtcggtaaac aatcgcattg atgttcatcc cggcaccaac cgaagcaaac
     4441 attatgatat cgcctttggc tatggt
//
LOCUS       DAOOQX010000073         4462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_23209_length_4462_cov_3.881552, whole genome shotgun sequence.
ACCESSION   DAOOQX010000073 DAOOQX010000000
VERSION     DAOOQX010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23209_length_4462_cov_3.881552"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLX53_01930"
     CDS             <1..334
                     /locus_tag="PLX53_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HOY71870.1"
                     /translation="VRQWNPYSLERWEDGIKNNSPNIEMESLSAVSKYNELLITRLRT
                     IWGVNTDDVSRMFGESFASFLNKSAQKYIDDGRMSRTKGNTLLISPSAYFVSDSIVEN
                     LMLIAEPD"
     gene            431..3553
                     /locus_tag="PLX53_01935"
     CDS             431..3553
                     /locus_tag="PLX53_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOY71871.1"
                     /translation="MKLVILLVFVIIHRNTFRFWIILTVAFIAVTALTIVPLVLAKKH
                     YAELIRREFNANIQEIHNRSTGYFQTIENGIANNLTLETYNDPLVSLKFTTDSYVRVQ
                     NVRREGKTVYSSPSDDNSEFYLFSESKVFPFEVATAVKRSDGFWGMKSFGFESYNVDY
                     LFRFDNLLSYLQGLTACNICMTLGDIQINPRTGSNSAAFRWNPYVLESGKWPAVVAQN
                     KQYFYVVNLAEVVDVSGNKAGMTFLAVDVSEIHRLFVSLVGLVSILGALSILLLGLFA
                     VKRYRFLKTAAKHEDNIKTNEEDLLRRASLVNLFDAMENGVVMINTDFEVVYINESYA
                     DFTGLQHSTTVGEKCYSALQGMYCHTAECPLSRILAGEPRVETTEFRFKSNGEKARFA
                     VATNPITDDSGKILGIIENFKDITEKLEVEQTLKQTEEQFEIFMNSFPLGVFIEDGIT
                     HEIIYQNAYLNDISNGACIKDFLPKEKSVKPVKSYRESTEEVMLVDSSGHWRYFLLHK
                     FKFIGVGNNLRIGGIMIDTTERREVERYRDVLSEAIDNSPVSIVILSPATEIEFVNPY
                     FLELTGYRIEEVYSKDINSLDLEYNSVKVLGKAVAAALGGRVWQGEVHLKHRNIGHSW
                     VGASFIPITDSTGSLQNIVGVMENITQHKEYEKEILLAKSKAEESDNLKSAFLSHLSH
                     EIRTPLNAIIGFSSLLSDSDLSLTERRNLSEIIYRNSNDLLRLIENLIEISEIETGTL
                     DIKKSQFSVNALMHSLYLQILEEDKKATNVRFSLRREVHHDDMVIYSDEAHIRQVLFH
                     LLVNSCKFTNHGFIEFGYSIVDEFTIMFYVLDSGVGIETDKQALIFNPFRQVDESSTR
                     QFGGMGLGLAISKHIVEKLGGKIWFNSTPGSGTNFYFTVPYIPVNSKFEVDNVGSRLQ
                     KFNWSSKTILVADDIDVNFVYLKAALKRTNAQVLWAKNGKEAVEIVRQGPESINLVLM
                     DLVMPDMDGFEATRLIKDFDSSIPVIGQTAYPNPGNAEAVNQYGLDSLLEKPIKINSL
                     LNELNKYLDN"
     gene            complement(3567..>4462)
                     /locus_tag="PLX53_01940"
     CDS             complement(3567..>4462)
                     /locus_tag="PLX53_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOY71872.1"
                     /translation="VLPASCNINKLISDILHQLTENREYMSRQVALKLALSLDDEQAI
                     MVTDADKLKKTLLHILGKLSKLAESDTLEIGYRVDSNASITFHVREVKQPFTDTSSNE
                     IILRQSVRSNGKHFTRQQGEAEIGMIIANGYAKALGGELDFGSTPDAGNVVYLRLPLN
                     ISKKKQSNELFCSPENWSGKTILVVEDDLMNFLYIEALLKRTNVRLIHAKNGEDAVEI
                     TLLHPEISLILMDLRLPFMDGYRVVREIRKQNHDIPIVATTASSMANEEQRSLDAGCN
                     AFITKPLDSDELYKTIRRYIR"
BASE COUNT         1243 a          941 c         1041 g         1237 t
ORIGIN      
        1 tgttaggcag tggaatcctt actcgctaga gcgctgggaa gacggaataa agaataattc
       61 gccaaatatt gaaatggaga gccttagtgc tgtttcaaag tataatgagc tgctcattac
      121 caggttacga accatttggg gtgtgaatac ggatgatgtt tcccgcatgt ttggagaaag
      181 tttcgccagc tttttaaaca agtctgccca aaagtatata gatgacggga ggatgagccg
      241 aaccaaaggc aatacactcc tgataagccc gtcggcctac tttgtaagtg atagcattgt
      301 ggagaattta atgttgatag cggagccaga ttaggttttg ctccacgcct gcaacccgaa
      361 gaggtggcga acaatctttt cgtaaaagta ttaggcagtg atgtggctaa tgaaataaat
      421 attcatattt ttgaagctag taatattgct ggtttttgtg attattcata gaaatacatt
      481 ccgattctgg attatactta cggttgcctt tattgctgta accgccctga caattgtccc
      541 gcttgttttg gccaaaaagc actacgcaga gctaatacgt agagagttta acgccaatat
      601 tcaggaaatc cacaaccgtt ctactggtta ctttcaaacc attgagaacg gcattgccaa
      661 taatttaacg cttgaaacgt ataatgaccc gctggtatcg ctaaaattta caaccgattc
      721 ctatgttcgg gtacaaaatg ttaggcgtga aggcaaaaca gtgtatagta gcccatcaga
      781 tgacaattcg gaattctacc tgttttctga aagcaaagta tttccatttg aggttgccac
      841 agcggttaag cgttccgatg gtttttgggg gatgaagtcg ttcggcttcg agagctacaa
      901 tgttgactat ttattccgat tcgacaacct tctctcatac ctgcaggggt taaccgcatg
      961 caatatctgc atgactttag gagatattca aatcaaccca agaaccggaa gtaattcagc
     1021 tgcatttaga tggaatcctt atgttctgga gtctggcaaa tggcctgctg ttgtagcgca
     1081 gaataaacaa tacttctacg tggtcaatct tgctgaagta gttgatgtat cagggaacaa
     1141 agccggaatg acatttttag cagttgatgt tagcgaaatt cacaggcttt tcgtgtcgct
     1201 ggttggcttg gtgtcaatat tgggagcgtt atctatcctg ctgttagggc tgtttgcagt
     1261 aaaacgatac cgttttctaa aaaccgcagc caaacacgag gacaatatca aaacaaatga
     1321 agaagattta ctccgcaggg catcgttggt gaaccttttc gacgcaatgg aaaatggggt
     1381 ggtaatgatt aacaccgatt ttgaggttgt ttacattaac gagagctatg ctgattttac
     1441 gggtttacaa cacagcacaa cggtagggga aaaatgctac agtgcacttc aggggatgta
     1501 ctgtcatact gccgaatgcc cgttgtcgcg aatactggcc ggtgagccaa gggttgaaac
     1561 aaccgagttc cggtttaagt caaatggcga aaaggcacga tttgctgtag ccactaatcc
     1621 aattaccgat gatagcggca aaattctagg gattattgag aattttaagg acatcaccga
     1681 aaagttggag gtggaacaga ccctaaagca aaccgaggag cagtttgaaa ttttcatgaa
     1741 ctcgtttccc ttaggcgttt ttattgagga tggaattacc catgaaatca tataccagaa
     1801 tgcctacctg aatgacatat caaatggggc atgcattaag gatttcttgc caaaggaaaa
     1861 aagtgtaaaa ccggttaaat cgtacaggga aagtaccgag gaggttatgc tggttgattc
     1921 atcggggcac tggcggtatt tccttcttca taagtttaaa tttattggcg tgggcaacaa
     1981 tcttcgtatt ggggggataa tgattgatac aactgagagg cgagaggtgg agaggtaccg
     2041 agatgtgctc tcggaagcga ttgacaattc gcctgtaagc attgtaatcc tttcaccagc
     2101 taccgaaata gagtttgtaa acccatactt cctggaacta accggatata ggatagagga
     2161 ggtttacagc aaggacatca acagcctcga cttggagtat aactctgtta aggtgctcgg
     2221 caaagctgtt gccgctgctt taggaggcag ggtatggcag ggcgaagttc acctcaagca
     2281 tcgaaatatt ggccatagtt gggttggcgc ttcatttatc cccataaccg attctacagg
     2341 cagcctgcaa aatattgttg gtgttatgga gaatatcacc cagcacaagg agtacgaaaa
     2401 ggaaatactg ctggcaaaaa gtaaagccga ggaatccgat aaccttaaat cggcttttct
     2461 ttcccatctt tcacatgaaa taagaacacc cctgaatgcc atcatcggtt tttcatccct
     2521 gctttccgac agcgacctca gcctaaccga acgacgaaac ctttcggaaa taatctaccg
     2581 taactccaac gatttgctca ggttgattga aaatctcatt gaaatttccg aaatcgaaac
     2641 cggaacgctt gatattaaaa agtcccagtt ctcggttaat gccctcatgc actcgctgta
     2701 cctccagatt ctggaggagg ataaaaaagc caccaacgtt cgttttagcc ttcgtcggga
     2761 ggtgcaccac gacgatatgg ttatatacag cgatgaggcg catatacgac aggtcctttt
     2821 tcacctgctg gtcaactcct gcaagttcac caaccatggt ttcatcgagt tcggatactc
     2881 cattgtggat gagtttacca tcatgttcta cgtcctggat agcggcgttg ggattgaaac
     2941 cgataaacaa gctctcatct tcaacccctt tcgccaggta gatgagtcga gcacccggca
     3001 attcggtggt atgggcttag gcctggccat ttccaagcat attgttgaaa agctgggcgg
     3061 taaaatatgg ttcaacagca ccccgggatc gggaacaaac ttttacttca ccgtgcccta
     3121 cattccggtt aactccaagt ttgaggtgga caacgtgggc agccgcctgc aaaaattcaa
     3181 ctggagctct aaaactattc tggtagccga cgatattgat gttaactttg tttacctcaa
     3241 ggcagcgctt aaaaggacaa acgcccaggt tttatgggca aaaaacggta aggaagccgt
     3301 agaaatagta agacagggtc cggagagcat aaacctggtg ttgatggatc tcgtaatgcc
     3361 cgatatggat gggtttgaag ccacgcggct tatcaaggat tttgacagca gcattccggt
     3421 aatcggacaa acggcatacc ccaacccggg gaatgctgag gcggtaaacc agtatggact
     3481 tgattcgctg ctcgagaagc caatcaaaat caactccctg ctcaacgagc tcaacaagta
     3541 tctggataac taaccctggt tgtaagctac cggatgtacc tccttattgt tttatatagc
     3601 tcgtcggaat cgagaggctt tgtgatgaat gcgttgcatc cggcatctaa gctacgctgc
     3661 tcttcattag ccatgctgct ggctgttgtg gcaactatcg gaatgtcatg attttgtttt
     3721 ctaatttccc ggacaaccct atatccatcc ataaatggga gtcgaagatc catgagtata
     3781 aggcttattt ccggatgaag tagggtaatt tcaactgcat cctccccgtt cttagcatgt
     3841 atcaacctca cgtttgtgcg tttcagcagg gcttcgatgt acagaaaatt catgaggtca
     3901 tcctcaacaa ccagaatggt tttaccactc caattctcgg ggctacaaaa caattcgttt
     3961 gactgtttct tttttgaaat attcaggggt agtcgtaggt aaaccacatt tcccgcatca
     4021 ggcgttgagc caaagtctaa ctctccaccc aacgccttgg cgtaaccatt ggcaattatc
     4081 attcctattt cggcttcacc ctgttgtctt gtgaaatgtt tgccattaga ccttactgat
     4141 tgtctaagaa ttatttcgtt tgatgatgtg tcggtaaagg gctgctttac ttcccgaaca
     4201 tggaaggtaa tgcttgcatt ggaatcaact ctgtagccaa tttcaagggt gtcggattcg
     4261 gcaagttttg ataattttcc cagaatgtgc agcagcgttt tcttgagctt atcggcatcg
     4321 gtaaccataa tggcctgctc gtcatcaagc gatagcgcaa gttttagggc tacctgacgg
     4381 ctcatgtact ccctattttc tgtaagttgg tgtaaaatat cacttattag cttattaatg
     4441 ttgcagctgg cggggagaac tt
//
LOCUS       DAOOQX010000074         4422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_23476_length_4422_cov_3.370048, whole genome shotgun sequence.
ACCESSION   DAOOQX010000074 DAOOQX010000000
VERSION     DAOOQX010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23476_length_4422_cov_3.370048"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PLX53_01945"
     CDS             complement(<1..804)
                     /locus_tag="PLX53_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOY71873.1"
                     /translation="MNSFGRIFRVSLFGESHGRAIGVVVDGVKPGLPLSTDDFADDIR
                     RRGPGLPGTTPRTENDLPEMVSGWFNGYTTGAPLAIIFRNTNVRSDDYERIKDLPRPG
                     HADFVAYKKFSGYNDYRGGGHFSGRITLALVAAGVIAKKMIDPVEVHAKLVTVGGSTD
                     IENTINKAMSANDSVGGLVECRVSGIPIGWGEPFFDSVESVISHLAFAIPGVKGIEFG
                     SGFAAASMFGSAHNDAFIDASGATSSNNAGGILGGISNGNELQFRVAVKP"
     gene            complement(820..2076)
                     /gene="aroA"
                     /locus_tag="PLX53_01950"
     CDS             complement(820..2076)
                     /gene="aroA"
                     /locus_tag="PLX53_01950"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOY71874.1"
                     /translation="MRRLVKPSLVSGAVQAPPSKSVAQRAIAMATLANGVTEIYNPGN
                     SNDSKTAVEICRALGASIAEYPGKLVVTGGLQQPSQILNCGESGLGIRMFSAIASTFS
                     AGVTLTGTGTLLKRPMQPIADALVQLGAACSTSSGFLPITVKGPINGGVANIDGSQGS
                     QVLTGLLVASPLAKHDVTIRATKLKSVPYVHLTIQMMHDFGVEVDVGSDIDFHVRAKQ
                     RYAPCTYNVEGDWSGTAFMLVAGATAGEVEVKNLNPSSLQADRAIVDALVMAGATVDV
                     EKNGVYVKRNKLSHFEFDATNCPDLFPPLVALASHCDGESKILGVSRLRVKESDRAAT
                     LVEEFAKLGIDIKIKDDLMVVQGGKVKSAWVHSHGDHRIAMACAVAALSGEGNLTIEG
                     AESVSKSYPRFFDDLNSIMTNSSLNV"
     gene            complement(2045..2980)
                     /locus_tag="PLX53_01955"
     CDS             complement(2045..2980)
                     /locus_tag="PLX53_01955"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY71875.1"
                     /translation="MTRKVYAVFGNPVLHSCSPQLFNSVFSEIPVEAYYTRIRPANAA
                     NIPELIRHLNLAGANITTPFKTEVMTHLDWLSDEAEIIGGVNTIINNQGYLKGYNTDY
                     LGVSRSLQEAGLHLQGSRCLVLGAGPAACAAVYALINEGADVTVVNRTNHKAKEIANR
                     FGCSSADMDKLDSAILECDILVSALLPEANPLANIKVPEWITAIDANYRASTLSQHLA
                     LSGCRTITGKRWLLHQAAASFNLFLNFNPKIELMAKGLEAQPQREAARYQMIGKDTLR
                     SIHTEDFDMLVSVPDSSTMTFRTILDEEISKAFVG"
     gene            complement(2973..3629)
                     /locus_tag="PLX53_01960"
     CDS             complement(2973..3629)
                     /locus_tag="PLX53_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOY71876.1"
                     /translation="MKILHPDICLSIGNMRYEDIFPYLKVAALAEIRLDLLNLTDEQI
                     RGLFTAHGNLIATYRCNDSAKMFQTLTNTLDSGAAFVDVDCLTENSIRDAIVAKAKGL
                     NRRVIISYHNFECTPNKEELERIIYSMKSQGADICKVACMANEPLDCSRVMALYEHIP
                     DLVAFCMGEAGKITRLAAPILGAPFTYASVAGASTAPGQLDYLKMEEYLNYFLPLNHD
                     "
     gene            complement(3705..>4422)
                     /locus_tag="PLX53_01965"
     CDS             complement(3705..>4422)
                     /locus_tag="PLX53_01965"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HOY71877.1"
                     /translation="MRGVRFGFVATSLLAQVDASIGGKNGVNLDGYKNIVGTFNQPQF
                     VICDTSLLKTLPETELSNGFAEMVKHALIADAPQFTILESNTNRLNTCDLELLDSLVA
                     SSVGVKASIVTADERESGERKKLNFGHTWGHAVEKVTGIPHGQAVSIGMAFASQLSAE
                     KGLLTPDECHRITSLLIALNLPVSIKANTNNIFEALQRDKKRCSNAIDFILLEGIGKA
                     VIKSLSLVEIKEFIERKYCY"
BASE COUNT         1092 a         1109 c         1025 g         1196 t
ORIGIN      
        1 gggcttaact gccaccctaa actggagctc gtttccgttg cttatgccgc ctaaaatgcc
       61 tccagcattg ttggatgagg ttgctcccga ggcatcaata aacgcatcgt tatgggcaga
      121 accaaacatg gaggcggctg caaaccctga gccaaactca atacccttaa cccccggtat
      181 ggcgaatgcc aaatggctga ttaccgattc caccgaatca aagaatggct cgccccaacc
      241 gataggtatg ccggaaaccc tgcactctac aagcccccca accgaatcgt tagccgacat
      301 ggccttgttt atggtatttt cgatatcggt gcttccgcca actgtgacca atttggcatg
      361 aacctccacc gggtcaatca ttttttttgc aataacgcct gccgcaacca gcgcaagagt
      421 gattctacca gagaaatgac cacctccacg atagtcattg tacccactaa attttttgta
      481 ggcaacaaaa tcggcatgac ccgggcgagg taagtcctta atgcgttcgt agtcgtctga
      541 ccgtacgttg gtatttctga aaataattgc cagcggtgct cctgtggtat agccgttgaa
      601 ccaacctgac accatttcgg gtaaatcatt ctcggttctg ggggttgtcc ctggtagtcc
      661 cggcccacgc cgacgtatgt cgtcagcaaa atcatcggtt gataggggca gccctggctt
      721 taccccatca actactacgc caattgccct tccgtgcgat tcgccaaata gcgatactct
      781 gaatatccta ccaaatgaat tcactggatt ataggttagt taaacattaa gcgagctatt
      841 agtcattatg ctgttcagat cgtcgaaaaa acgggggtag ctcttcgata ctgactcggc
      901 tccctcgatg gtcagatttc cttcacccga aagtgcagca actgcacagg ccattgcaat
      961 gcgatgatcg ccgtgcgagt gaacccatgc tgatttcacc tttcctccct gaacaaccat
     1021 caaatcgtcc ttaatcttga tgtcaatacc cagcttggca aattcctcca ccagggttgc
     1081 agccctgtcg ctttctttta cccttaatcg gctgacgcct aaaattttag attcgccatc
     1141 gcagtgtgag gcaagtgcaa ccagtggggg aaacaggtca ggacagttgg tggcatcgaa
     1201 ttcaaaatgg ctgagcttgt tacgtttaac ataaacgcca tttttttcaa catccacagt
     1261 agcgccggcc atcacaaggg catcaacaat tgccctatcg gcctgcagcg acgaggggtt
     1321 aagatttttc acctctactt cgcctgccgt agcaccggca acaagcatga atgcagttcc
     1381 gctccaatca ccctcaacgt tgtaggtgca gggagcataa cgctgttttg cccttacgtg
     1441 aaaatcaata tccgatccaa catctacctc aaccccaaaa tcgtgcatca tttgaatggt
     1501 gaggtgcaca tagggaacac ttttgagttt cgttgccctt atggtaacat cgtgtttggc
     1561 cagtggtgaa gccacgagta gcccggtgag cacctgcgag ccttgcgagc catctatgtt
     1621 ggctactccg ccattaattg ggcctttcac ggttatgggt agaaatccgg atgaggtgct
     1681 gcaggcagct ccaagttgaa ccagcgcatc agcaatgggt tgcataggcc tttttaggag
     1741 cgttccggtt ccggttaagg taacgccggc actaaatgtt gaggcaattg ccgaaaacat
     1801 tctaataccc aaaccggatt cgccacaatt aaggatttgg gagggttgct gcaatccgcc
     1861 cgttaccacc agtttaccgg ggtattcagc tatgctggcg ccaagcgccc ggcaaatctc
     1921 aactgctgtc ttagaatcgt tactgttgcc aggattgtat atttccgtaa cgccattggc
     1981 taaagttgcc atggctattg ccctttgggc aacgcttttc gagggggggg cctgcacagc
     2041 tccgctaacc aacgaaggct ttactaatct cctcatctaa aattgttctg aatgtcatgg
     2101 ttgagctgtc gggaaccgat acgagcatgt cgaaatcttc cgtatgaatc gaacggagtg
     2161 tgtcttttcc tatcatttgg tacctagcag cttccctttg gggttgtgcc tcaagtcctt
     2221 ttgccattag ctcaattttc gggttgaaat ttagaaaaag gttgaatgag gctgctgcct
     2281 ggtgtagcag ccagcgttta cccgtaatcg ttctgcagcc gcttaatgcc aggtgttgtg
     2341 atagcgttga ggcacggtag ttggcatcaa ttgccgtaat ccactccgga accttaatgt
     2401 ttgcaagtgg atttgcctcg ggaagaagcg ccgaaaccag aatatcgcat tcgagaatgg
     2461 cgctgtccag tttatccata tcagctgagc tacagccaaa ccgatttgct atttcttttg
     2521 ctttgtggtt tgttctgtta acaaccgtta cgtctgcgcc ctcattaatc aaggcataaa
     2581 ctgcagcaca tgcagcaggc cctgcgccca aaacaaggca tcggctaccc tgaagatgaa
     2641 ggcctgcttc ttgaagcgaa cgactcacac ccaggtagtc ggtgttgtac cccttcaggt
     2701 aaccctggtt gttaattatg gtatttacac cacctatgat ttctgcttcg tccgaaagcc
     2761 aatcgaggtg cgtcattact tcggttttaa aaggcgtggt gatgtttgca ccagccagat
     2821 tcaagtgcct tatcaactcg ggaatgttgg ctgcatttgc aggtcggata cgggtgtagt
     2881 aggcttcaac gggtatttcg ctgaaaaccg agttgaaaag ctgggggctg cagctgtgga
     2941 gcacaggatt cccaaaaacg gcatacacct tcctagtcat ggtttaacgg taaaaaataa
     3001 ttcaggtatt cctccatttt caagtagtcg agttgcccgg gagcagttga tgcgccggca
     3061 accgaagcgt aggtaaatgg tgcgcccaga atcggagcgg ctagccgggt aatttttcct
     3121 gcttcaccca tacaaaaagc tactaaatcc ggtatgtgct catacaaagc catcaccctt
     3181 gagcaatcaa gcggttcatt ggccatgcat gcaaccttac aaatatcggc accctggctt
     3241 ttcatgctgt aaattatcct ttcgagttct tccttgttgg gtgtgcattc aaagttgtgg
     3301 tatgagataa taacccttcg gtttagccct ttagccttgg caacaatggc atctctgatg
     3361 gaattttctg taaggcagtc aacatcaaca aaggccgcac ctgaatcgag tgtattggta
     3421 agtgtttgaa acattttagc gctgtcattg cacctataag ttgcaatcag gttcccatgt
     3481 gcagtaaaaa gccctcgtat ctgttcatct gtaagattca ggaggtcgag ccgaatttcg
     3541 gcaagggcag ctaccttcag gtaggggaaa atatcctcat acctcatgtt tcctatgctc
     3601 aagcaaatat cggggtgcaa aatcttcata gccgttttct catcagtaat atgctgtcgc
     3661 aacttgtata aaaagcagga atagtcaact tttaactttt cttttcagta acaatacttc
     3721 ctttctataa actccttaat ctcaacaagg cttaggcttt tgataacggc ctttccaatg
     3781 ccctcaagca ggatgaagtc tatagcgttg gagcatcgtt ttttatccct ctgcaatgcc
     3841 tcaaaaatat tgttggtgtt ggcttttatg gaaacaggta gattaagcgc aataagcaag
     3901 ctggttattc gatggcattc atctggtgtg agcaaccctt tttcggccga gagctgcgag
     3961 gcgaatgcca ttcctatgct aacggcttgt ccgtggggga tccctgttac cttttcaact
     4021 gcatggcccc atgtgtggcc aaagttaagc ttctttcgtt cgccgctctc tctctcatcg
     4081 gcggttacaa ttgatgcctt aaccccaacc gatgaggcaa ccaacgagtc gagtaattct
     4141 aaatcacagg tgttaagcct attagtgtta ctttccaaga tggtaaattg gggagcatcg
     4201 gcaatcaggg catgttttac catttcggca aaaccgttgg acagctcggt ttcgggtaac
     4261 gttttaagaa gcgaggtatc acaaatcacg aattgcggct ggttgaatgt tcccacaatg
     4321 ttcttatagc catcgagatt cactccattc ttaccaccaa tgcttgcgtc aacctgggcg
     4381 agcaggctgg tggccacgaa gccaaaccta accccccgca tg
//
LOCUS       DAOOQX010000075         4374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_23785_length_4374_cov_3.436907, whole genome shotgun sequence.
ACCESSION   DAOOQX010000075 DAOOQX010000000
VERSION     DAOOQX010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23785_length_4374_cov_3.436907"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="PLX53_01970"
     CDS             <1..1292
                     /locus_tag="PLX53_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71878.1"
                     /translation="LDVRTNNIKVYEVFPGADNLNPMFSGNGHTIYFLSNSDGYRNLF
                     KYDLNTGETYRLTRILTGISGMTPFAPAMSVAHENDEICYSHFYNSKYSIYTADTTDF
                     NPVKVDPYHVDFSASILPPLNRATEGLVDQNLTSNDLPEHLVDSFKTVKFRPQFKLDY
                     ISNIGMGISTSPYGTGMAGGVEMLFSDITGAKMLYTGLSLNGEVYDFGGQVSYINQRK
                     YITWGVTLSHIPYSFGAYGYDTLHRVDNGNPIIIEDFQVLQYRMFETAGGGMAYFPLS
                     QTQRFELTASVARYYYRVDIYSNYYVDGFYIGADRHKGDAPPGFWMQQTNLAYVYDNS
                     DFGIASPMRGNRVRLQVDKTLGDYNYWGSLVDLRKYIFAKPFSFAFRGMYYGRYGTSI
                     ENSVLYPLYIGYPWYVRGYDSNVLLTTGSREPPALTS"
     gene            1217..1618
                     /locus_tag="PLX53_01975"
     CDS             1217..1618
                     /locus_tag="PLX53_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOY71879.1"
                     /translation="MVREGLRQQCFIDYGQSGTTSIDQLTGDQMVVTNFEVRVPFTGP
                     ERLTLIKSGAFYTELALFADAGIAWSKGYPPSLKWKPTSLNDRTPFVSAGLSIRINLF
                     GMMVLEPYYAIPFQLGGFKSANWGINFLPGW"
     gene            1881..3089
                     /locus_tag="PLX53_01980"
     CDS             1881..3089
                     /locus_tag="PLX53_01980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOY71880.1"
                     /translation="MVRRIKKLPITIFTSETAPAFAGDGLNALLLAESLIKKGYSAQL
                     ICLNPNKLLPKSEVINGVTIRRIAYHYKNKRGRLWLRVNMLLYILGAARKQGYWLIYG
                     AMPANRLLVLTGWILGKKVFFRSTLYSFDDASTLISSKKNPVKAIYRFIYGRVAGYHA
                     LNSVFAEAWRKTYNEIIPIFVSPQGTTIPYPAPNIQNRKNARSELGLPPDPIIVLMVG
                     HLITRKGFPDIFEWLAKANCSFLLLHVGTIHAPESNIISIRNNEMRQNIVAGQNLLGE
                     RVKFMECSQRPSLYYYAADILLLASAAEGYPPNTVNEAMAAGLPILSNRIAGSTDYIS
                     DNHNGFLYTGFDEFISRFSQLVSNQDIRQQLGNNARLYAEKYLDVNPIAENLLKFMDE
                     SAARHSRHSH"
     gene            complement(3046..4215)
                     /locus_tag="PLX53_01985"
     CDS             complement(3046..4215)
                     /locus_tag="PLX53_01985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOY71881.1"
                     /translation="MQANSYSKEKYPGELKKKGILFIVQLPPPVHGASMMNSMVYSRL
                     LGDLRYRCRLVRLNFARNLNDLQKFRFRKLGRSFTVFLEICWKALTFQPSIIYFSMVP
                     LGTTLLRDSAYLLAVRLLLPKSRKIIHLHRPGLYEFNQKYRLSWFYRFIFKGCEIIHL
                     TESLVEHEITPLQLTNVKVYAVPNSIEVDAAQHDAGVARMRYNILFLSNLFPHKGFIP
                     LVKAHALLKNNFPGLTLTIAGEIPNSDVEGRLRETIRELCLGDSVKVLGKVSGEQKSL
                     LYRQAGIFVLPSEQEYFPLVILEAMAARTPVITSGRHNLESLFTDMHDLLFVDDLSPE
                     GIAKTIQKLLDNPALASSIGENGYHRFCELQVQSQRMMEDLFNGCADYALLPTRP"
     gene            complement(4170..>4374)
                     /locus_tag="PLX53_01990"
     CDS             complement(4170..>4374)
                     /locus_tag="PLX53_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71882.1"
                     /translation="YSRRVSPKKVTKFVIDNVKPGSIVVFHDSLKAERNLRYALPRVL
                     DAITATGMVCKPIVIPRKNIRES"
BASE COUNT         1248 a         1090 c          938 g         1098 t
ORIGIN      
        1 cacttgatgt gagaactaac aacattaagg tttacgaggt atttccagga gccgacaacc
       61 taaatcccat gttctcgggt aacggccaca ccatctactt cctctccaac agcgacggct
      121 accgcaacct cttcaagtat gacctcaata cgggcgaaac ctaccgcctg acgagaatcc
      181 taactggaat atcggggatg accccctttg cacctgccat gagcgtggcc cacgaaaacg
      241 atgaaatctg ctactcccat ttttacaaca gcaagtactc catctacacc gccgatacca
      301 ccgactttaa cccggtgaag gttgatccgt accatgttga cttcagcgcc tcaattctac
      361 caccacttaa ccgggccacc gaagggcttg ttgaccaaaa cctaacaagc aatgatcttc
      421 ctgaacacct ggttgactca tttaaaacgg taaagtttag accccagttc aagctcgact
      481 acatctccaa cattggaatg ggcattagca cttcgcccta tggaacagga atggcgggtg
      541 gcgtggaaat gctcttcagc gatatcaccg gcgccaagat gctctacacc ggcctatcat
      601 taaatggcga ggtttatgac ttcggtggac aggtgtcata catcaaccaa agaaaataca
      661 taacctgggg tgttaccctt tcgcatatcc cctactcctt tggagcttat ggctacgaca
      721 ccctgcatcg agttgataat ggtaacccga taatcataga ggattttcag gtgttacagt
      781 accgcatgtt tgaaacggca ggaggaggta tggcctactt cccgctttcg caaactcaaa
      841 gatttgaact cactgcttcg gtagcacgtt actactacag ggttgacatc tactcaaact
      901 actatgttga tggcttctac ataggagccg acaggcacaa gggcgatgct cctccgggtt
      961 tctggatgca acaaaccaac ctggcttacg tttacgacaa ctccgatttt ggcatagcct
     1021 cgcccatgag gggaaaccgt gtcaggcttc aggttgacaa aaccctgggc gattacaact
     1081 attggggtag cctggttgac ctgaggaaat acatatttgc caaacccttc agctttgcat
     1141 tcagagggat gtactacgga agatacggaa catctattga aaacagcgtt ctatacccgc
     1201 tttacattgg ctacccatgg tacgtgaggg gttacgacag caatgtttta ttgactacgg
     1261 gcagtcggga accaccagca ttgaccagct aaccggcgac caaatggtgg ttacaaattt
     1321 cgaggtaaga gtgccgttta ccggtcccga aaggttaacc cttattaaat ccggggcatt
     1381 ttataccgaa cttgccctat ttgccgatgc cggcatagcg tggagcaaag ggtacccacc
     1441 cagcctaaag tggaagccta cctccctcaa cgaccgcact ccctttgtta gcgccggcct
     1501 aagcatacga ataaacctgt ttgggatgat ggtgcttgag ccctactacg ccattccatt
     1561 tcagctgggg ggattcaaat cggccaactg gggtatcaac ttcctgccgg gatggtagcg
     1621 attctttttt tctcataaat aaaaaagtat gactcggatg acgcggatgc gacggaaaat
     1681 cacagattaa tcagttcaaa tcagtttaat ctgtgttttc cgtgtaccat taaattcgat
     1741 aggacacgaa cgatactgat agtatgaaaa aattgttgta ttagaataaa tatttaacgc
     1801 attcaacaag taaggtatcc acttttattg cctacacttt tttcaaacca aaaaatctac
     1861 cttaacacca gcctctagca atggtgcgaa gaataaagaa gttgcccatt accatcttta
     1921 cctccgaaac tgcacccgca tttgccggtg atgggctgaa tgccctactt ctggctgaat
     1981 ccctgataaa aaaagggtac agtgcgcagc taatctgcct aaaccctaac aagcttctac
     2041 ctaaatcaga ggttatcaat ggggttacca ttagaagaat agcctaccat tacaaaaaca
     2101 aaagaggtag attatggcta agagtgaaca tgcttcttta cattcttggt gctgccagaa
     2161 agcaaggcta ctggcttata tacggagcaa tgccggcaaa ccgtctgcta gtactcaccg
     2221 gttggatact tgggaaaaag gtttttttcc ggtcaaccct ctactctttt gacgatgcct
     2281 ccacgctaat ttcgagcaaa aaaaatcctg tcaaagccat ctacaggttc atttatggta
     2341 gggtagcagg ataccatgcg ctaaacagcg tctttgcaga ggcatggaga aaaacctaca
     2401 acgaaatcat tccgattttt gtatcgcccc agggaaccac cataccatat cctgcaccaa
     2461 acattcaaaa cagaaagaat gcccggagtg agctgggctt accccctgat ccgataattg
     2521 tactaatggt cggacacctg ataacccgaa agggttttcc cgacatattc gaatggttgg
     2581 caaaggcgaa ttgtagcttt ctgctcctac acgtgggaac cattcatgcc ccggagtcga
     2641 acattatctc aatccgcaac aacgaaatga ggcagaacat agtagcaggg caaaacctgc
     2701 tgggtgaaag ggttaagttt atggaatgca gccagcgccc ctcactctac tactatgctg
     2761 cggatatatt acttctagcc tcggctgcag aaggctaccc cccaaatacg gtaaacgaag
     2821 ccatggcagc aggattgcca attctctcaa acaggattgc aggctcaacc gactacattt
     2881 ccgataacca caatggtttt ctgtacacgg ggtttgatga attcatttcc aggttcagcc
     2941 agctggtctc caatcaggat attcgccagc agctcggcaa taacgcccgg ctgtatgccg
     3001 aaaagtatct tgatgtcaat cccatagccg aaaacctact taaattcatg gacgagtcgg
     3061 cagcaaggca tagtcggcac agccattgaa taaatcttcc atcattcttt ggctctgcac
     3121 ttggagctca caaaaacggt ggtaaccatt ttcgccaata ctactggcaa gtgctgggtt
     3181 gtccaacagc ttttgaattg ttttagcaat gccttcgggt gaaagatcat caacaaacag
     3241 caggtcgtgc atatcggtaa acagcgattc caggttatgc ctaccacttg taatcactgg
     3301 ggttcgggca gccatagcct caagaattac cagcgggaaa tactcctgtt cggagggcag
     3361 cacaaaaatg cctgcctgac ggtaaagtaa tgatttttgc tcaccggaaa cctttcccag
     3421 aactttcacg ctatcgccaa ggcaaagctc acggattgtt tcccgcaatc taccctcaac
     3481 gtcggaattg ggaatctctc cggcaatagt aagcgtcagc cccggaaaat tgttcttcaa
     3541 caaggcatgg gccttcacca gcggaataaa acccttatga ggaaaaagat ttgagagaaa
     3601 caggatatta tacctcatcc tggcaacgcc ggcatcatgc tgtgctgcat caacctcaat
     3661 ggagttgggc acagcataca cctttacatt tgttagttga agtggcgtaa tctcatgttc
     3721 taccagtgat tcggtaaggt gtattatttc acatccttta aatataaacc tatagaacca
     3781 tgaaagcctg tatttctgat taaactcgta gagtccggga cggtgaaggt gaataatttt
     3841 tctcgactta ggaagtaaaa gcctaacagc cagcaagtat gcgctatcgc gaagaagtgt
     3901 agttccgagc ggaaccatcg agaagtatat aatggaaggt tgaaaggtta atgccttcca
     3961 gcatatctcc agaaaaacag taaaagaacg acctaacttc cgaaaccgaa acttctgcag
     4021 atcgttcaga tttctagcaa aatttaacct gaccaaccta cagcgatacc ttaggtcacc
     4081 caacaacctg ctgtacacca tgctgttcat catgcttgct ccgtgaacag gtggtggcag
     4141 ctgaacgatg aatagtattc cctttttttt caactctccc ggatattttt ccttggaata
     4201 actattggct tgcataccat cccggttgca gtaatggcat ccaaaacccg tggcaaggca
     4261 tacctcagat tcctttcagc cttaagagaa tcgtgaaaaa ccactattga acctggcttc
     4321 acattgtcta tgacaaattt tgtaaccttt tttggcgaaa ccctccggct gtag
//
LOCUS       DAOOQX010000076         4365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_23851_length_4365_cov_3.316009, whole genome shotgun sequence.
ACCESSION   DAOOQX010000076 DAOOQX010000000
VERSION     DAOOQX010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23851_length_4365_cov_3.316009"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PLX53_01995"
     CDS             <1..649
                     /locus_tag="PLX53_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cell envelope biogenesis protein OmpA"
                     /protein_id="HOY71883.1"
                     /translation="GHASYRIEYGGMFATTISLAYRGFQQGRWSYTYSNDLNNDGISS
                     DLIYVPKDKDEALAMFVPYTPTGGVEMTAQQQADAFWAYVNGNDYLKDRKGKYAERFG
                     DVQPWLHRFDVKVLQDIFSNFGTSRRYTLQISLDLLNVGNMLNDKWGAYTYNPLASYD
                     NVRPLTRVALPTATTAPTFRLNANTIEEFETKTTLSKNISTTSTWGALLGIRLIF"
     gene            1238..3796
                     /locus_tag="PLX53_02000"
     CDS             1238..3796
                     /locus_tag="PLX53_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013760737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY71884.1"
                     /translation="MMKVIKKILLTVCIVGTAGVAFSQGIVKGVVVDSNSGEALVGAA
                     VAVENTTIGTLTSLDGSFVLKVPVEKGTIKVTFVGYSDKTIEVTIPSGTEKDLGQIQL
                     TASSIGLEEVLVVSSFVRDRTTPVAVSTIEPKIIAEKLGNKEFPEILKSTPSIYATKS
                     GGGFGDSRVYVRGFDSNNVGVLINGIPVNGMEDGKVYWSNWAGLSDVTSNQQVQRGLG
                     ASKLALSSVGGTINIITRSTDAEKGGSVYAGIGNDGYMKQTFTVSTGMLDNGWAMTLS
                     GGHTYGNGYVQATNFEGWSYFVNVSKKINSKHSIVFNAFGAPQWHNQRSNKHTIQEFR
                     DHPMEAKWNSDFGYRNGDIYNIGYAYNYYHKPQISLNHYWKISDNAMLSTQAYASIAT
                     GGGRRVYGPNSTWLSLNYPSGTPYDNSALTPEGYFDFDWVIAQNAASTTGSKAIIANA
                     INDHQWYGVLSTFTTSISDFKITAGFDGRYYSGVHAYKIDDLLGGVYYLDNKNINRPA
                     SQPLHKGDYVNYNYRGQVLWLGLYAQAEYIKNSYSGFLSVAAASNNYRRVDFFQYAPG
                     NQKSPWHDFLPWNIKGGFNYKISESRNVFINGGYIKKAPIFSNSFLNNTNDFNKGVKY
                     EKIITAEVGYGFNLRNFNAKLNVFRTSWLDKGLVRSLNGQTANILGINALHQGVELEC
                     YYKPINTLNVKGMFSYGDYRWTDDVNFTLYNEDQSVAGTYNAYIADVHVGNSAQITAA
                     VGVDYEVLPKLKVGVDFNWFGKNYADFDPTARTTIESKVDAWEMPDYYLLDIDANYRF
                     KMGSFNATIYGNVSNLLDTEYIADATDGTYHDEFSSYVFYGFGRTWSAGLRINF"
     gene            3920..>4365
                     /locus_tag="PLX53_02005"
     CDS             3920..>4365
                     /locus_tag="PLX53_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71885.1"
                     /translation="MKRILNLTMLLGLLWACNPMEDTYNNLDEARKPYKENITYTLDA
                     TDYSNASKYALADAQNHADSASAQLIKDQQAFNQRFTAKNYVGKVLANRFVALNLGST
                     ANVTYNVNLENTGYLTAYSSPTLYTLTSDNYQSVGGAVANTGYFIPS"
BASE COUNT         1300 a          914 c          939 g         1212 t
ORIGIN      
        1 tggccatgcc tcctacagaa ttgagtatgg tggaatgttt gccactacta tttcgttagc
       61 ctatagagga tttcagcaag gacgctggtc ctatacctac tccaacgact taaataacga
      121 tggcatatcc tccgacctga tctatgttcc aaaggacaag gacgaagctc tggccatgtt
      181 tgttccctat accccaacag gaggtgttga aatgactgca caacaacagg ctgatgcttt
      241 ttgggcttac gttaacggta atgattacct gaaagatcgt aaaggcaagt atgcagaacg
      301 ttttggtgat gttcaaccat ggcttcacag gttcgatgta aaggtgctgc aggatatttt
      361 ctccaacttt ggaaccagca ggagatatac actgcaaata agccttgact tgctcaacgt
      421 tggtaacatg ctaaacgaca agtggggcgc ctatacctac aacccgctgg ctagctacga
      481 caatgtccgt cctttaacca gggttgccct gccaaccgca actaccgctc ctacattcag
      541 gcttaatgcc aacacaattg aagaatttga gactaaaaca accctcagca aaaacatcag
      601 cacaaccagt acatggggtg ctctacttgg tataaggttg attttctaaa agccatgttt
      661 acttaataaa taatccgtcc gggttttctg gacggatttt tttatcattt tacccaatca
      721 tttatccggc ttatcctata tgctcacacc aatgattgct ttaaccttat gagttaaacg
      781 taacctctta aatttttgca ttgtgcatta agtactaata gactcatata tgttgtttta
      841 attatttgaa tctaggcgtg ttagcattta ccctcatagt ttaaatgttt tcaaaacgcc
      901 atgttctaag ggcttgacct agaaaataaa tcaaccatat tggaattact gttaaagttt
      961 attccaaatt ttagttgatg gtaaagaaag gttagccgta taatacactt tgaaattcat
     1021 gtttgtaatt tggtgcacaa aaattcagct taaacactaa aggaacaacg ctttcggtta
     1081 aacgggggtc ggtttcctgc aagcgagaag tggaggttag tattttatta atattcaccc
     1141 ttaagccatc aggttatttt tcacaacttc aatacgcaaa caaaattgta gatcacaatc
     1201 aatagctaca cttaacccta accaaattca taacagtatg atgaaagtta ttaaaaaaat
     1261 cctacttacc gtttgtatag taggaacagc aggagtagcc ttttcacagg gtattgtgaa
     1321 aggtgttgtt gttgacagca acagtggcga agcgttagtt ggcgctgcag tagcagtcga
     1381 aaacacaaca attggaacgt taacctcgtt ggatggttct tttgtattaa aagtacctgt
     1441 agagaaagga accatcaagg ttacatttgt tggttattcc gataaaacaa ttgaggtaac
     1501 cattccctcg ggaacagaaa aggatttagg gcaaatacag cttacggcca gctcaatagg
     1561 gctggaagag gtgctggtgg tttcgtcgtt tgttcgtgac cgaaccacac cggttgccgt
     1621 ttcaaccatt gagcccaaaa tcattgccga aaagcttggg aataaagaat ttcccgaaat
     1681 tcttaaatca acgccttcaa tttatgcaac caaaagcggt ggaggttttg gcgattcccg
     1741 cgtttacgtt aggggttttg actccaacaa cgtgggcgtg ctaattaatg gaattccggt
     1801 aaatggcatg gaggatggca aggtgtactg gagtaactgg gctggtcttt cggatgttac
     1861 ttccaaccag caggttcaac gtggtctggg tgcatccaaa cttgcccttt cctcggttgg
     1921 aggaacaatc aacattatca ccaggtcaac agacgctgaa aagggcgggt cggtatatgc
     1981 gggcattggg aacgatgggt atatgaagca aacttttacg gtatccaccg gcatgttgga
     2041 taacggttgg gctatgactc tttcgggggg acatacatac ggcaatggct atgttcaggc
     2101 tactaatttt gaaggttggt catattttgt taatgtttcc aaaaaaatta acagtaagca
     2161 tagcatagtt ttcaatgcat ttggagcacc tcagtggcat aaccagcgca gcaacaagca
     2221 tactattcag gaattccgcg atcaccccat ggaggctaag tggaattcgg atttcgggta
     2281 ccgcaatggc gatatttata acattgggta tgcctacaac tactaccaca aaccgcaaat
     2341 ttcattaaac cactactgga aaattagtga taacgccatg ctatctaccc aggcttatgc
     2401 ctcaatagca accggaggcg gaagaagagt ctacggtccg aattctacct ggttgagctt
     2461 aaactacccc agcggaacac cttatgataa ctctgcgctc actcccgagg ggtattttga
     2521 ttttgactgg gttatagcac aaaatgccgc ttcaacaacc ggttcaaaag ccataatagc
     2581 caatgcaatt aatgaccatc agtggtatgg agtgctttca acttttacca catctatttc
     2641 agattttaag attaccgccg gttttgacgg acggtactac agcggtgtac atgcatataa
     2701 aattgacgat ttgcttggtg gggtttacta ccttgataat aagaacataa atcgtcctgc
     2761 atcacagccg cttcataagg gcgattatgt taactataac tataggggac aggtgctgtg
     2821 gttaggactt tatgcacagg cagagtatat aaagaatagc tactcgggat tcctttctgt
     2881 tgctgctgct agcaacaact accgtagggt tgattttttc cagtatgctc ctggaaatca
     2941 aaaatcccca tggcacgact ttctgccctg gaatattaaa ggaggtttta actacaagat
     3001 aagtgaaagt cgaaatgtgt ttattaacgg tgggtacatt aaaaaagccc caattttttc
     3061 caactccttc ctgaataata cgaacgattt caacaagggc gttaagtacg aaaagattat
     3121 tactgccgag gtaggctatg ggtttaactt acgaaatttc aatgccaagc tgaacgtgtt
     3181 ccgtaccagc tggttggata aagggcttgt aagatcattg aatggccaaa ccgccaacat
     3241 tctgggaatt aatgcgcttc atcagggagt cgagttggaa tgctactaca aaccaattaa
     3301 taccctgaat gttaagggaa tgttctccta tggagattac cgttggacag atgatgttaa
     3361 ctttaccctt tacaacgaag accaatcggt agccggaaca tataacgcat acattgctga
     3421 tgttcatgtg gggaactctg cccaaataac agcagctgtt ggggttgact atgaggtgct
     3481 gccaaagcta aaggtgggcg ttgactttaa ctggtttggt aaaaactatg ccgattttga
     3541 tccaactgcc agaacgacta ttgaaagcaa ggtggatgca tgggaaatgc ccgactacta
     3601 ccttcttgat atagacgcca actaccgttt caaaatggga tccttcaatg ctaccatcta
     3661 tggcaacgtg tcaaacttgc tggacaccga gtatattgcc gatgcaacgg atggaacata
     3721 tcatgatgag ttttcatcgt atgttttcta tggctttggt agaacgtggt ctgccggact
     3781 tagaatcaac ttttaacacg atggatagtg cacgcgcaaa ttttaggagt tgagttgata
     3841 tgttgaagat ttgcaagccg ttgcataacc gtaataattt aaccaaacga atttaaataa
     3901 acaataaatg ataacaccga tgaaaaggat tctcaatctt accatgctgt tgggcctttt
     3961 gtgggcatgt aaccccatgg aggacaccta caacaatctt gacgaagcca ggaaacccta
     4021 taaggaaaac atcacctata ctcttgatgc aaccgattac tctaatgcaa gcaagtacgc
     4081 tttggctgat gcccaaaatc atgccgacag cgcatcggca cagctgatta aggaccagca
     4141 ggcattcaac caaaggttta cagccaaaaa ctatgtcggg aaagtgcttg ccaacagatt
     4201 tgttgcgcta aacctaggct ctactgcaaa tgtgacctac aacgttaatc ttgaaaacac
     4261 cggttacctt actgcctaca gcagcccaac actttacacc cttacctccg acaactatca
     4321 atcggtagga ggtgctgttg caaataccgg ttatttcata ccatc
//
LOCUS       DAOOQX010000077         4358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_23901_length_4358_cov_3.391820, whole genome shotgun sequence.
ACCESSION   DAOOQX010000077 DAOOQX010000000
VERSION     DAOOQX010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23901_length_4358_cov_3.391820"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLX53_02010"
     CDS             complement(<1..440)
                     /locus_tag="PLX53_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71886.1"
                     /translation="MKSFTTTILILGLLLLQPHSTQAQCKNFAKKICKLELAPYVHDG
                     NYNAAILTEGEDAELFKTFYAGQEYRISICGSESLAKIEFLVMDVDRRVLYSNAKNNY
                     ATSWNFKVEATQQLIILVRVPSSGNSGSELASGCVAIMFGLKAE"
     gene            complement(457..1374)
                     /locus_tag="PLX53_02015"
     CDS             complement(457..1374)
                     /locus_tag="PLX53_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71887.1"
                     /translation="MATGFVKRIVKLLGLLLIVGGVACKSEKPSDKSLKVTEDELAAD
                     SVISQSIQSVKQIFYSLPSPLETALILKRSGAVYNEDLLNPVENVAHYNTSKSMALNL
                     GIYSTDLSYASLFDQTQATINLMNASKKMADGLGILNAIDNSVIQRLEENINNRDVIM
                     ETISETFLNTNSILEEDDRVAIGSIILVGGWLEGLYLATSLVSDEKKMDNELVDRIAD
                     QKFSLETVLSLLKQNSDNADVMEIYSDMQKLSEIFYDIQISASRVEVVPASNNGATIL
                     RSKNVITVNPDVFNKLKTKVKELRTKYTA"
     gene            complement(1594..2109)
                     /locus_tag="PLX53_02020"
     CDS             complement(1594..2109)
                     /locus_tag="PLX53_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71888.1"
                     /translation="MLAEILKIVLPALMVFLAGYLAISAMIKSETNRRKSELMFNNIK
                     ITTPVRLQAYERLVLFLERISPESLLPRTTKKGMLASDLHTALLGTIRAEWEHNLSQQ
                     LYVSEEAWEMVKNAKENIIKLINVCADRINPNETSMALSQKIIETYVELDTNPTTLAI
                     DFLKKEVAEIF"
     gene            2198..3391
                     /locus_tag="PLX53_02025"
     CDS             2198..3391
                     /locus_tag="PLX53_02025"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOY71889.1"
                     /translation="MEKVSSRIAALEESQTIAMSQKSRELAAQGIDVVNLSLGEPDFF
                     TPDFIKEAAKRAIDENFSFYPPVSGYKDLLEAICGKLKRENDLTFTPDQVVVSAGAKH
                     SLSNVLMCVVEKDDEVVVPAPYWVSYVELIKLAEGRSVIVPTTIENDFKITPQQLEAA
                     ITPKTRAFLFCSPSNPTGSVYSKAELEAIARVIEQHPNIIVISDEIYEHINFVGKHES
                     IAQFDSIRDRVVIINGVSKGYAMTGWRIGFSASAKWIAKAAAKLQGQQTSGVCTIAQK
                     AATAAFKSDGQTTRQMCEAFKRRRDLFVKGISGINGFKINVPQGAFYLFPDITHFLGL
                     HFNEQVVATADDFAMYLLNNAHVAVVSGSAFGSPYNIRLSYATSDEKLSMAVERIAQA
                     VKLLR"
     gene            3430..>4358
                     /gene="mltG"
                     /locus_tag="PLX53_02030"
     CDS             3430..>4358
                     /gene="mltG"
                     /locus_tag="PLX53_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOY71890.1"
                     /translation="MSQPQLKTFSRRWLPIVLALAGLFVGFLVLYRYVTTFRVDNVAL
                     THDTYLYIPTGSDFDEVVRDMEGKGLLKNVGSFIKVSRKMGYDLHVKPGRYRVTPDMS
                     NQKLVNKLLMGAQDPVRVTFHNIRTKQQLSGRISKQLEADSLSILTMLRSQVDAMAFG
                     FDTATIMAMFIPNTYEFYWNTDANDFFTRMKKEYESFWTPEREQKAYRLGITRIDVSI
                     LASIVEEETRMVTEMPVIAGVYINRLHRGIPLQADPTVRFALGDFSIRRILSKHLQLD
                     SPYNTYKYKGLPPGPIRCPSIRAIDAVLNAEKH"
BASE COUNT         1133 a          955 c          987 g         1283 t
ORIGIN      
        1 tactcggcct tcaacccgaa catgattgct acgcaaccac ttgccaactc cgagccagaa
       61 tttcccgatg aaggcactct caccaaaata atcagctgct gggttgcctc aaccttgaag
      121 ttccatgatg tggcatagtt atttttggca ttggagtata gcaccctgcg gtcaacgtcc
      181 atcaccagaa attctatttt agccagggat tcggagccac aaattgatat tctgtattcc
      241 tggcctgcat aaaaggtttt aaatagctcg gcgtcctcac cctcggttag aattgctgcg
      301 ttgtaattcc catcatgaac gtacggagcc aactccaact tgcaaatttt ttttgcaaag
      361 tttttacatt gggcttgtgt gctgtggggt tgaagtagca gtagccctag aataagaatt
      421 gtagtggtaa atgatttcat accggaatgt gttatgctat gctgtgtact ttgtacgcaa
      481 ttccttaact tttgttttaa gcttgttgaa aacatcaggg ttaacggtaa tgacattttt
      541 tgaacgcagg atggttgccc cgttgttgct agcgggaaca acttcaaccc gagatgcaga
      601 aatttgaatg tcataaaata tttcgcttag cttttgcatg tcggagtaaa tttccataac
      661 atcagcgtta tcggaattct gctttaaaag gcttaaaacg gtttcaaggg aaaacttctg
      721 atcggcaatt ctatccacca gctcattatc catcttcttt tcatcgctaa ccagcgaggt
      781 ggccaggtaa agcccctcca accatccgcc taccagaatt atagatccaa tagcaacccg
      841 gtcatcttcc tccaggatgg agttggtgtt aaggaatgtt tcggatatgg tttccattat
      901 tacatcgcgg ttgttgatgt tttcttcaag ccgttgaatt accgagttat caatggcatt
      961 cagaatgcct agcccatctg ccattttctt tgatgcattc atcaggttaa tcgtggcctg
     1021 ggtttgatcg aacaggctgg cgtagcttaa gtcggtgctg tagattccca ggtttaacgc
     1081 catgctttta ctggtgttgt aatgcgcaac gttttcaacc gggtttagca ggtcttcatt
     1141 gtatactgcg cctgaccgtt ttagtattag ggccgtttcg agtggggagg gcagggagta
     1201 gaatatttgc ttaaccgatt ggatgctttg agaaatcacg ctatcagcgg caagttcatc
     1261 ctccgtgact tttaacgatt tgtccgaggg tttctccgat ttgcaggcta ccccgcctac
     1321 tatcagcagc agtcctaata actttactat tcgcttgacg aaacctgtag ccatatcacc
     1381 aacttttaat tgtcaatttt acccacattt tcagtaagta aagataaaat aattttttac
     1441 tatattccta tagaaattca ttgttgaatt caaataattg caaaaaagtt gataaccagg
     1501 gttgattttc aaccattttg ttgtttggat ggaatcaagc tatgtcggtc cagtaaatgc
     1561 aacatgcaga aaaataattc ctctgcttaa ggcttaaaaa atttcggcta cctccttttt
     1621 taggaaatca atagcaagcg tagtggggtt agtatcaagc tccacgtagg tttcaattat
     1681 tttttggctc aaggccatgc tggtttcgtt ggggtttatc ctgtcggcac aaacgttgat
     1741 aagcttaata atgttctcct tggcattttt aaccatctcc caggcttcct cgcttacgta
     1801 gagctgctgc gataggttgt gctcccattc agcccttatg gtgcccagca atgccgtgtg
     1861 caggtcggaa gccagcatcc ctttttttgt agttcttggc agcaacgatt cgggtgatat
     1921 tcgttcgagg aaaagcacaa gcctctcgta ggcttgcagc ctcactgggg tggttatctt
     1981 tatgttatta aacatcagct cagatttcct gcggttggtt tcgctcttaa tcatggccga
     2041 tatggcaagg tatcccgcca gaaaaaccat tagagcgggc agcactatct tcagaatttc
     2101 tgctagcatg ggttaggatt ttaagggaaa gtttgactat ttttgagttc ttaaaattac
     2161 aaaaagaatt ttgaatttat tttaaatcta tacggttatg gagaaagtat cttctcgaat
     2221 agcagcattg gaggaatctc aaaccattgc catgtcgcaa aaatcacggg agttggcggc
     2281 acagggaatt gatgtggtta acctcagctt gggtgaaccc gattttttta ctcccgattt
     2341 tattaaggag gccgcaaagc gcgctattga tgagaacttt tcgttttacc ccccggtttc
     2401 ggggtacaag gacttgctgg aggcgatatg cggaaagctg aagcgggaga atgatcttac
     2461 ctttaccccc gaccaggttg ttgtttcggc tggtgctaag catagcctgt caaatgtgct
     2521 aatgtgcgtt gttgaaaagg atgatgaagt ggttgtccca gcaccctact gggttagcta
     2581 cgttgagctt attaagctgg ccgaagggcg gagcgttata gttccaacca ccattgagaa
     2641 tgattttaag attacccctc agcagcttga ggcagcaata actccaaaaa ccagggcatt
     2701 tctcttttgc tccccttcaa accccacagg aagcgtttac tccaaggctg aacttgaggc
     2761 cattgccagg gttattgagc agcacccaaa tatcatagta atttccgatg aaatttacga
     2821 gcatatcaac ttcgtgggaa aacatgagag cattgcccag tttgatagca ttcgcgacag
     2881 ggtggtcatc attaacgggg tgtcgaaagg ctacgccatg accggttggc ggattggatt
     2941 ttctgcgtcg gctaaatgga tagccaaggc tgctgccaag ctacagggac aacaaacttc
     3001 gggagtatgc accattgccc aaaaagctgc cactgctgcc tttaaatccg atggtcaaac
     3061 cacccgccaa atgtgcgagg cgtttaaaag acgccgcgac ctgtttgtaa agggaatttc
     3121 gggtatcaac ggtttcaaaa taaatgttcc tcagggcgca ttctacctct tccccgacat
     3181 tacgcacttc ctgggtttgc actttaatga gcaggttgtt gctactgctg acgattttgc
     3241 catgtatctg ctaaacaacg ctcatgttgc tgttgtatcg ggctcggctt ttggttcgcc
     3301 ctacaatatt cgtttgtcct atgctacgtc tgacgaaaaa cttagcatgg ctgttgagcg
     3361 cattgcccag gctgttaagc tactacgcta gcaacttgtt gagcttattc aacctaaaac
     3421 tgatgtaact tgagccaacc ccagctaaag actttttcca gaagatggct gcctatagtt
     3481 ttggcattag caggattgtt tgtgggcttt ttggtgcttt accgctatgt tactactttc
     3541 cgtgtcgaca atgttgcgtt aacgcacgat acatacttgt acatacccac cggatccgat
     3601 tttgatgaag ttgttaggga catggaaggt aagggtctcc taaaaaacgt tggtagcttt
     3661 ataaaagtct ccaggaagat gggttacgac ctgcatgtta agccaggacg gtaccgtgta
     3721 actcctgaca tgagcaacca aaagctggtg aacaagcttt taatgggagc gcaggaccca
     3781 gtgagggtta catttcacaa tatcagaacc aagcagcagc tctcgggacg aatttccaaa
     3841 cagctggagg ctgattcgct ttccattttg accatgctga ggtcgcaggt tgatgccatg
     3901 gcattcggat ttgatactgc aaccataatg gccatgttta ttccaaacac ctatgaattc
     3961 tactggaata ccgatgccaa cgactttttt acaaggatga aaaaggagta tgaaagtttc
     4021 tggacacctg aacgcgagca gaaagcctat cggcttggga taacaaggat agatgtttct
     4081 atattggcct caattgttga ggaggaaacc agaatggtaa ctgaaatgcc tgttattgca
     4141 ggagtttaca ttaataggct gcatagggga atcccccttc aagccgatcc aaccgttagg
     4201 tttgcccttg gcgatttttc catcagaaga attctttcca aacaccttca actcgattcg
     4261 ccctataaca cgtacaagta caagggactt ccaccaggcc caattagatg tccctccatt
     4321 agagctattg acgctgtgct aaatgccgag aaacatag
//
LOCUS       DAOOQX010000078         4332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_24095_length_4332_cov_2.907178, whole genome shotgun sequence.
ACCESSION   DAOOQX010000078 DAOOQX010000000
VERSION     DAOOQX010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24095_length_4332_cov_2.907178"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLX53_02035"
     CDS             complement(<1..510)
                     /locus_tag="PLX53_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743650.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOY71891.1"
                     /translation="MKHGKVLLMILDGWGIGNKDKSDVIYSTPTPNIDTLTAAYPNAQ
                     LLACGENVGLPDGQMGNSEVGHLNIGAGRIVYQDLVRINKDIKENKLSKMPVLVEALS
                     YAKSNNVSVHFIGLVSDGGVHSLDKHLYNLLDITKEYGLSRVFIHALTDGRDTDPQSG
                     LGFIRNLENH"
     gene            953..3757
                     /locus_tag="PLX53_02040"
     CDS             953..3757
                     /locus_tag="PLX53_02040"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HOY71892.1"
                     /translation="MKHFRKTDYRAGTQNWGISQDKQGFMYFANNNGLLRFDGNSWDL
                     FPIPGNSLVRSVLCINDTIYVGAFEEFGYYTYSNGNDLTYTSLTQKIAGKAWGIDEVW
                     RIYKYNNTILFQTFTYIMVLEKGEISAFPTPEVLQFSFLIGNDFFVQGKNGKIYKFLQ
                     NRFEPIDDGGKFAGKQIWAVLKQENGSTLLATINHGVWEYNNGTVKAWNNAANSYLIK
                     NQIFTASTLRGGYIAFGTIQAGLVVTDEKGEIVLLIDKKRGLQNNTILSLYTDLHNNL
                     WLGLDNGIDYMELNSPISIIDEGLGLFGSCYSTILYNNKLFVGTNQGLYVCDWPIRKN
                     DIGGSFPFQRVEKTNGQVWCLEVIDGKLYCGHNFGTFEIDSKTNVEHTSTEEGSWTFL
                     KLKNHPNKMLVGKYKGIHLYVKENGSWRFLKKVKGFDESCRFIAEDENENIWMAHGYK
                     GVYRLVLNEEKDSVKYSYLFDNTKGFPVKTGINMEKLRNQIIFITQQGVFKYNALIDR
                     MEPYSEFNRKLDDTINIRRLTEDSEGNIWILKSNDVKILKQQPDGKTSLEPTPLVLFG
                     NSFVSSYENIYIPDISNVFLGTENGLIHYNPSYPTADSIKFKCYIKSMQTLSRKGEKI
                     FTDYKINRTQPISIKYSNNSIGLTFTSPTYNAGEIAYSYKLEGYDTGWSEWSSNPIKE
                     FTNLPYGNYTFRLKALDAKGRESDEVELKFTISNPWYLSLWAFIAYGITCVLITLILR
                     AAVKAKIRNATTKVKEQKNLEMKVKEEEHKREVLEAEQEIIRLQNENLKVQVEHKNSE
                     LASIALQIAHKNEFLNQLKCKLEALTKNTNPALQKEVLELINKIDFDLRMDDEWKRFE
                     FHFDEVHVGFLKRLKDLYPTLSPNELRLCAYLRLNMTTKDIAQILNISIRGVEISRYR
                     LRKKLGIEGDTNLIDFMINL"
     gene            complement(3780..>4332)
                     /locus_tag="PLX53_02045"
     CDS             complement(3780..>4332)
                     /locus_tag="PLX53_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY71893.1"
                     /translation="FSNSVVLRADLFDTDNRLTDVSPVFKFSPVEPGGQFHVYSFDFK
                     NKWGSNSPEYGQVVNQHQVKGVRFYVDYGIYGKVAADSLWLSDIAISTHPLSASYIQQ
                     KSQFLSVYPNPCNDYICLYFDSQVVTTYQVSIYTIEGSLAGYFESSQNPCFISTKQLP
                     SGVYVVTTLNSEGFSARVKLVKL"
BASE COUNT         1402 a          873 c          903 g         1154 t
ORIGIN      
        1 atggttctcg agatttctga taaagcccag accgctttgt gggtctgtat cgcggccatc
       61 ggtcagagca tgaatgaata ctcttgacag gccatactcc ttagtaatat caagcaggtt
      121 atacaaatgc ttatcgagtg agtgaactcc gccatcggaa accagaccaa taaagtgaac
      181 gctaacatta ttactcttgg cgtagctaag agcctcaacc agcacgggca ttttgctcag
      241 cttgttttcc ttaatatcct tgtttatccg caccagatcc tggtaaacta ttctaccagc
      301 gccaatgttc aggtgtccaa cctccgaatt tcccatttgg ccatcaggta ggccaacgtt
      361 ttcaccacat gccaggagct gggcgtttgg gtaagctgcc gttagggtat caatatttgg
      421 cgttggggtg ctgtatatta cgtccgattt atccttattt ccaatacccc agccatcaag
      481 aatcatcagt aaaacctttc cgtgtttcat atcctgtaag ttatattttc atttaaaacc
      541 acacaccagg aacgttttca gtaaccttta tgttcgtaca cgactacaaa gttacccttt
      601 tttaattgcc ttcggctaat gttggcttat tattgaccgg ttgtggcgta aacgtttgaa
      661 aattgtactg acacctatca gcttaccctg ctatttgttg aaaaattaac cgttcgtagt
      721 aattttttaa taattttatc ccaaatgatg aaaggttgtt attgccagat ttaaaaatcg
      781 aacggagcat ataaaccaaa gtccattaga aacatagccc ataaaaatga acttttctag
      841 gagtattaaa tcaaccagca taaccaaaag gagcatccct ctgcttacca cattattaat
      901 attcactttt actctaggcc attcacagat aattaccaca ggattgcctg taatgaagca
      961 tttccgaaaa actgactaca gggcaggcac gcaaaactgg ggtatttcgc aggataaaca
     1021 aggttttatg tattttgcca ataacaatgg cctgctccgg tttgacggga atagctggga
     1081 tcttttcccc atccccggaa acagcctggt tagatcggtg ctgtgtataa acgacaccat
     1141 ttatgtaggg gcattcgagg aatttggcta ctacacctac agtaatggca acgaccttac
     1201 ctatacttcg ctcacccaaa aaattgcggg aaaggcgtgg ggaatagatg aagtttggag
     1261 aatttacaag tacaacaaca ccattctttt tcaaacattt acctatatca tggtactcga
     1321 aaagggtgag ataagcgctt ttcccactcc cgaagtgctc cagttttcat tcctcatcgg
     1381 gaacgatttt tttgttcagg ggaagaatgg taaaatctat aaattcctgc aaaatagatt
     1441 tgagccaata gacgatggtg gaaaatttgc cggaaagcag atttgggcag tactaaaaca
     1501 agaaaatggt tcaaccttgc tggcaacaat aaaccacggc gtatgggagt ataacaatgg
     1561 gactgttaag gcatggaaca atgctgcaaa tagctattta attaaaaatc aaattttcac
     1621 agcttcaacg ctgcgcgggg gatacatagc ttttggaacc atacaggcgg gtttggtggt
     1681 aaccgacgaa aagggagaga ttgttctact aattgacaag aagagagggc ttcaaaataa
     1741 caccatttta agtctttaca ccgacctgca taacaactta tggctaggac tagataatgg
     1801 cattgactac atggagctga actcgcccat atctattatt gacgaaggtt tggggttgtt
     1861 tggttcttgc tactcaacaa tactgtataa taataagctg tttgtaggaa ccaaccaggg
     1921 gctctatgtt tgcgactggc caattaggaa aaatgatata ggtggaagtt ttccttttca
     1981 aagagtagag aaaactaacg gacaagtgtg gtgtcttgag gtgattgatg ggaaactata
     2041 ctgtggccat aactttggga ctttcgaaat tgattccaaa acaaatgtag agcatacttc
     2101 caccgaagag gggtcgtgga ccttcctgaa gctaaaaaat catccgaata aaatgctggt
     2161 tggaaagtac aagggaatac atctatatgt aaaggagaat ggttcgtggc gatttcttaa
     2221 aaaggttaaa ggttttgatg agtcatgccg ttttattgct gaggatgaaa atgaaaatat
     2281 atggatggct catggttaca agggtgtgta tcggcttgtg ctgaatgaag aaaaggatag
     2341 cgtaaaatac tcctaccttt tcgataacac caaagggttt ccggttaaaa cagggattaa
     2401 catggaaaag cttcgaaacc aaataatctt cattacccaa cagggagtat tcaagtataa
     2461 cgcactaata gacaggatgg agccatattc cgaatttaac cggaaactgg acgatacaat
     2521 taacataaga cgattaaccg aggatagcga gggcaatata tggatactaa aatcaaacga
     2581 tgtaaaaatc ctaaaacagc aaccggatgg caagacatcc ctcgagccta cccctcttgt
     2641 actttttggc aattcctttg ttagctctta cgaaaatatt tacattcctg atatttcaaa
     2701 tgttttcctt ggaactgaaa atggattaat tcactataac ccaagctacc ccactgccga
     2761 cagcattaaa tttaaatgct acatcaaaag catgcaaact ctttcccgca agggtgaaaa
     2821 gatctttacg gactataaaa ttaacaggac gcaacctatc agcataaaat actccaacaa
     2881 ctccattggg cttacgttta cctcgcccac atacaacgca ggtgaaattg catatagcta
     2941 caagctcgaa ggttacgaca ccggatggtc cgagtggagc agcaatccca taaaagaatt
     3001 taccaaccta ccttacggaa actacacctt taggctaaaa gcattggatg ccaagggtag
     3061 agagagcgat gaagttgagc ttaagtttac catcagcaac ccatggtacc tttcgctttg
     3121 ggcatttata gcatacggaa taacctgcgt tctgataacc ctcatactta gagcagcagt
     3181 taaggctaaa attagaaatg ctaccactaa ggttaaggaa caaaaaaacc ttgaaatgaa
     3241 agttaaggag gaggagcata aaagggaggt tctggaagca gagcaggaaa taataaggct
     3301 acaaaatgaa aatttaaagg ttcaggttga acataaaaac agcgagttgg ctagcattgc
     3361 gctccaaatt gcacacaaaa acgaattttt aaatcagcta aaatgcaagc tggaggctct
     3421 tacaaaaaat acaaacccgg ccttacaaaa ggaagtgctg gagctgataa ataagattga
     3481 ttttgactta aggatggatg acgagtggaa gcgctttgaa tttcacttcg atgaagtcca
     3541 tgttggattt ttgaaacgcc tgaaagacct ataccctacc ctttcaccca acgaacttag
     3601 gctttgcgcc tatctcaggc taaacatgac tactaaggac attgcccaaa tactgaacat
     3661 cagcataaga ggagtggaaa ttagccgcta ccggttgcgc aaaaagttgg gcatcgaagg
     3721 cgatactaac ctgatagact ttatgataaa cctttaggga aaagaggatt cagcgagtac
     3781 tatagcttca cgagtttaac tcttgctgaa aaaccttcac tattgagagt ggtaacaaca
     3841 taaacccctg aaggaagttg ctttgtggaa ataaagcatg ggttctgact tgattcaaaa
     3901 tagcctgcca aagatccctc aatagtgtaa atgctgacct ggtaggttgt aactacctga
     3961 ctatcaaaat aaagacaaat gtaatcattg catgggttag gataaacgct taaaaattga
     4021 gacttctgct gaatatagct tgctgaaaga ggatgggtgc tgatagctat atcgcttagc
     4081 catagggaat cagctgccac cttgccataa atgccatagt caacgtaaaa cctcacgcct
     4141 ttaacctggt gctggttaac tacctgaccg tattccggtg agttggaccc ccacttgttt
     4201 ttaaaatcaa aggagtaaac atgaaattga ccaccaggct caaccggaga aaacttaaag
     4261 acaggcgaaa catcggttag cctgttatcc gtatcaaata aatcggctct tagcacaacc
     4321 gagttgctaa ag
//
LOCUS       DAOOQX010000079         4332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_24096_length_4332_cov_2.626140, whole genome shotgun sequence.
ACCESSION   DAOOQX010000079 DAOOQX010000000
VERSION     DAOOQX010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24096_length_4332_cov_2.626140"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PLX53_02050"
     CDS             <1..897
                     /locus_tag="PLX53_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOY71894.1"
                     /translation="DIPVYHPDCEVYEVQEADGRLLGILYLDFFPRDSKSGGAWCTEY
                     RSYKVKPDGTISTPVVSIVTNFTKPAGDTPALLSFDEAETFFHEFGHALHSLFSKVQY
                     PGLANVPQDFVELPSQIMEHWASHPKYLKEYALHYKTREPMPDKLISKIEASGQFNQG
                     FATVEYLAASILDMDYHTLTAEKAKIEDVLAFEKASMDRIGLIPEILPRYRSTYFGHI
                     FSDVLGYSSGYYSYIWAEVLDSDAFEAFVESGDIFNKEVAGKFRTCVLEKGGSKEALE
                     LYKDFRGREASIEPLLKNRGLK"
     gene            1200..1955
                     /gene="pssA"
                     /locus_tag="PLX53_02055"
     CDS             1200..1955
                     /gene="pssA"
                     /locus_tag="PLX53_02055"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOY71895.1"
                     /translation="MPTGKSITKQLPNVITLLNLVSGCLSIVAALDCDLQLAGLFIIL
                     AAVFDFLDGFTARLIGAYTMLGKELDSLSDVVSFGVAPSMIIYELMRQSLELPKNEGL
                     LNGHWLLLIPFVMAAFSSLRLGMFNLDERQSSSFIGLPTPANALMIVGLTFGLNSTWA
                     DVYGIFTSSVWALTAIVALQSFLLVCELPMFSFKIKSLKLSVAYKQLLFLSGAAVLVV
                     IFLKAALSLIIVWYVFLSLVFYLTGKMGKKQHV"
     gene            2059..2316
                     /gene="purS"
                     /locus_tag="PLX53_02060"
     CDS             2059..2316
                     /gene="purS"
                     /locus_tag="PLX53_02060"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOY71896.1"
                     /translation="MKFTAEINVMPLKALLDPQGKAVNSTLHGIGHTEVSNVRIGKHI
                     TMEVESASREEAIEKVTEICSKVLSNPVMEGYDFTLNEIKS"
     gene            complement(2318..3430)
                     /locus_tag="PLX53_02065"
     CDS             complement(2318..3430)
                     /locus_tag="PLX53_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOY71897.1"
                     /translation="MDTPLNITPISSWFEGLSLPLIIAGPCSAESEEQVMDAALQLAG
                     IERVKVFRAGIWKPRTRPGSFEGAGEKGLRWLQRVQRETGLLVTTEVATPAHVQMALD
                     YGIDIVWIGARTTASPFTTEQLAVALSHSNIPVLVKNPITPDLELWIGALERFNRMGI
                     TKLAAVHRGFYPYEHSRLRNIPKWELAIDLKSRFPDLPILCDPSHIAGRADMVAQVAQ
                     HGLNLSLDGLMVEVHPNPSAALSDARQQVSPHFFSQLLQKLIFRDSSSACSEYVDFIE
                     QTRNKIDSIDQQIIELLANRMKLVEQIGEYKRDNNVTVFQLRRWERILESRVEYGERL
                     GLSEEYIKNLLRMVHKESIRRQAEILGSKSQPNSNE"
     gene            complement(3506..>4332)
                     /locus_tag="PLX53_02070"
     CDS             complement(3506..>4332)
                     /locus_tag="PLX53_02070"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY71898.1"
                     /translation="KFSPDELATIAGKLFGNALQVVVGCGAEPTMYAHFVDIVKKASE
                     YSVPHISLVTNGQLLTRDHLNELAESGLNELVVSVHGASQQTYEHLMVGASWEKLHKL
                     LNSVNEMRAVNRYPNFTLRVNYTVNPLNLHELETFFDAFGQYQINTLQIRPVMKIGGE
                     YEDGFSQSDIHTYSKIIDKVRNYCREQGITLLANTDDPTYQRSSKVSWVLNETYKYVS
                     PAMVVSSDFKWKEETLSAYLRRTGWYATMLKRIFWFQRAEKPDDLAQKYSARYDLL"
BASE COUNT         1105 a         1074 c         1003 g         1150 t
ORIGIN      
        1 gatatccccg tttaccatcc cgactgcgag gtttatgagg tgcaggaggc cgatggccgg
       61 cttttaggaa tcctctacct cgatttcttc cccagggata gcaagtcggg tggggcctgg
      121 tgcaccgagt accgttccta taaggtcaaa cccgatggca ccatttctac ccccgtggtg
      181 tccattgtaa ccaactttac caagccagca ggtgatactc ctgccctgtt gagcttcgac
      241 gaagccgaaa ctttctttca tgaatttggt catgccctgc acagcctgtt ctctaaagtg
      301 caataccccg gactggctaa tgttccgcag gatttcgttg agctacctag ccaaatcatg
      361 gagcattggg ccagccatcc gaaatacctg aaggagtacg cccttcacta caagacaagg
      421 gaacccatgc ccgataagct gatttcaaaa attgaagcca gcggccaatt caaccagggc
      481 tttgcaacgg ttgagtatct ggctgcatcc attcttgata tggactacca cacccttacc
      541 gctgaaaagg caaaaattga agatgtgctg gcctttgaaa aagcctcgat ggatagaatt
      601 gggttaatac ccgaaattct accccgctac cgcagcactt actttgggca tattttcagc
      661 gatgtgctgg gctactcaag cggatactac tcctacattt gggccgaagt gctcgatagc
      721 gatgcctttg aagccttcgt ggagagcggc gacatattca acaaggaggt agcaggaaaa
      781 ttccgcacct gtgttctcga aaagggtggt tccaaggagg ccctggagtt atacaaagat
      841 tttcgaggtc gtgaagccag cattgaaccg cttctaaaga acagaggatt gaaatagcac
      901 gccattacac ataacaccaa aaggggctca aaccgagccc cttttttagt tgcaagtcat
      961 actaacggtt gctgcctggt aaagctcatc aatctccggt gttagcttaa tcccgacata
     1021 ttcaagtcaa cgtaatgagc attcatcggc atcccgccta atgaaaaatt tctgaaatgc
     1081 tttctgcaac agttttacct ttttaccatt taacaaaccc atgaattttc attcatttag
     1141 aattttattt ttgaacttga cgaatttgta ctctaatttt agccccaaat tatagcgcca
     1201 tgccaacagg taaaagtatt actaaacagt tgcccaacgt aatcaccctg cttaacctgg
     1261 tttcgggatg cctttcgata gtggcagcgc ttgattgtga tctccagctg gcagggctgt
     1321 ttatcatttt ggcagcggtg ttcgactttc tcgatgggtt tacggccagg ctgataggtg
     1381 cgtacaccat gctggggaag gagctggact cgctctcgga cgtggttagc tttggcgttg
     1441 caccctctat gatcatctac gagctgatgc ggcaaagcct ggagctgcca aaaaacgaag
     1501 gtctcctgaa tggccattgg cttctcctaa tcccatttgt aatggctgcc ttctcgtcgt
     1561 tacgattggg aatgtttaac cttgatgagc ggcagtcatc atcattcatt gggttaccca
     1621 cccctgccaa tgcgctgatg atagtaggcc ttacctttgg cctcaacagc acatgggctg
     1681 atgtgtacgg aattttcaca agttcggttt gggcattaac cgccattgta gccctccagt
     1741 cgtttctctt ggtttgcgag ttgcccatgt tctcgttcaa gataaaatcg cttaagctga
     1801 gcgttgccta caagcagcta ctttttcttt ccggcgctgc cgttctggtt gtgatatttc
     1861 tgaaggccgc actttcgctc attatcgttt ggtacgtatt tctatcgctg gttttctacc
     1921 ttaccgggaa aatggggaaa aagcaacatg tttgagctaa attcaaccgg tgattctaaa
     1981 aatcccctat ttttgtcatc ataaatcaag tagtcctgct aaattttatt gtatagccgg
     2041 ctaaataagt tcaaacgcat gaaatttact gccgaaatca acgttatgcc gctaaaggcg
     2101 ctactcgacc cacagggaaa agctgttaac tcaactcttc atggtattgg gcataccgag
     2161 gtgtcgaatg ttagaatagg taagcatatt accatggagg tggagtcggc tagccgggag
     2221 gaggccattg aaaaggtaac ggagatttgc tcaaaggtgc tctcgaaccc ggtgatggag
     2281 ggttacgatt tcaccctaaa cgagattaag agctagccta ctcgttgctg ttgggctgag
     2341 attttgaacc taaaatttca gcttgccttc tgatggactc cttatggacc atgcggagga
     2401 ggtttttgat gtactcttcg ctcaacccta acctttcccc atactccacc ctcgattcga
     2461 gtatgcgttc ccaccgacgc agctgaaaaa ccgtaacgtt gttatcacgc ttgtactcac
     2521 caatttgctc caccagcttc atcctgtttg caagcagctc gataatctgc tgatcaatgg
     2581 agtcaatttt attacgggtt tgctcaatga agtcaacata ctcagagcat gccgacgagg
     2641 aatccctgaa aatcaacttt tgcagtagtt gcgaaaaaaa atggggactt acctgctgac
     2701 gggcatcgct aagggcagcc gatggattgg gatgaacctc caccataagc ccatcaagcg
     2761 aaagattcag gccatgctgg gccacctgtg caaccatatc ggcacgaccc gcaatatggc
     2821 tgggatcgca taaaataggt aaatcgggga atcggctttt caaatctatg gccagctccc
     2881 atttaggaat gttccgtagg cgtgaatgct cgtagggata aaaaccgcga tgcacggcag
     2941 ccagcttggt aattcccatg cggttgaagc gttcaagcgc acctatccac agctccaggt
     3001 ccggcgtaat gggatttttc accagcaccg gaatgttact atggctaagg gctacagcaa
     3061 gctgctcagt tgtgaacggg ctggctgttg ttcgggcgcc aatccaaact atgtcaattc
     3121 cataatcgag ggccatctga acatgagcag gagtggccac ctcagtagtt accagtaagc
     3181 ccgtttcgcg ttgaaccctc tgaagccatc gtaaaccctt ttcgccggca ccctcaaagc
     3241 tgccagggcg ggtgcgaggc ttccatatac ctgccctgaa aacctttacc ctttcaattc
     3301 cggcaagctg aagggctgca tccataacct gctcctcgct ttcggcacta cagggtccgg
     3361 caataattaa cggcagcgat aacccctcaa accaactcga aattggcgtt atgttaagtg
     3421 gtgtatccaa actttaaccc aataaatatt tgacaaagtt agtgagaaat aatcaactgg
     3481 taagggtaaa actaacaacc agctgctaaa gcaggtcgta acgggcgcta tacttttggg
     3541 ccagatcatc aggtttttca gcacgctgaa accaaaaaat tctcttaagc atggtggcat
     3601 accatccggt tcttcgcagg taggccgaaa gtgtctcttc cttccactta aaatcgctac
     3661 taaccaccat tgccggagaa acgtacttgt aggtttcgtt cagaacccag ctgactttag
     3721 acgaccgctg gtaggtggga tcatcggtgt tggccagtag ggtgatgccc tgctcccgac
     3781 aataattccg gactttgtca ataattttgc tgtaggtgtg aatgtcgctt tgggagaacc
     3841 catcttcata ctcgcctccg attttcatca ccggacgaat ctgaagggtg ttaatttggt
     3901 attgtccaaa agcatcgaaa aaagtttcca gctcatgcag gtttaacggg ttaactgtat
     3961 agttaactcg aagggtaaag ttggggtagc gatttaccgc tctcatctcg ttaaccgagt
     4021 tcaaaagctt gtgtagcttt tcccagctag ctccaaccat aagatgctcg taggtttgct
     4081 ggctagcccc atgaaccgac acaaccagct cgttgaggcc cgattcggca agctcgttca
     4141 aatggtcccg ggtaagtagt tggccgttag ttaccaagct gatgtggggc actgagtact
     4201 cactggcctt tttaacaata tccacaaaat gtgcatacat cgttggctcg gcaccacaac
     4261 ccaccacaac ctgcagcgca ttgccaaata gcttccctgc aatagtagcc agctcatcgg
     4321 gtgaaaattt tt
//
LOCUS       DAOOQX010000080         4299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_24322_length_4299_cov_2.821631, whole genome shotgun sequence.
ACCESSION   DAOOQX010000080 DAOOQX010000000
VERSION     DAOOQX010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24322_length_4299_cov_2.821631"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..633
                     /gene="rplS"
                     /locus_tag="PLX53_02075"
     CDS             268..633
                     /gene="rplS"
                     /locus_tag="PLX53_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOY71899.1"
                     /translation="MDLIKVAEESFASKKEHPSFKSGDTVVVHYKIVEGSKERIQNYR
                     GVVIQRRGVGATETFTVRKISGGVGVERIFPLNSPFIEKIEVAKEGKVRRARIYYLRN
                     LTGKKARIKEKKRKLDIGE"
     gene            937..1010
                     /locus_tag="PLX53_02080"
     tRNA            937..1010
                     /locus_tag="PLX53_02080"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:971..973,aa:Arg,seq:acg)
     gene            complement(1073..3961)
                     /locus_tag="PLX53_02085"
     CDS             complement(1073..3961)
                     /locus_tag="PLX53_02085"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOY71900.1"
                     /translation="MLKAKILMGSLLIAASLAGISQEVQMDNSRVKQLTLNNGLTVIL
                     DENHAQPKVFGLVMVKAGGKNDPADATGMAHYQEHMLFKGTQTLGTINWKEEKPYIDK
                     IFQLYDSLGKTSDEAKRKQIQQEINEASLKANSFAIPNEFSNLVNEIGGSMLNAGTGP
                     DYTVFYNMFPSSQIERWLDLYAHRFVEPVFRGFQAELEVVYEEKNLYNDQFFTKLLEE
                     FQKQFFKNHPYGQQTLIGSIEDLKNPSLIKMYDFFKTYYVPNNMALVLSGDFNSDEII
                     PVIEEKFGKWKEHELPESKIWTEKPFNGREFYQAKLSPVKMGLLGFRAPSAKDDGFLA
                     AEVATRILNNSYSTGLLDKLTLDGKLLAAQALQMPYLDHGAVIVVMLPKILGQSLEDA
                     ESLVNNELGKLKRGEFDDWMLEAVKLDLYRNHVTTMEKIDGRALYLGEAFAKGQSAQE
                     ALDYPNKIMRLTKDDVVAAANQIFGDSYLAFYSKMGFPKKEKIEKPDFKPLLANTEAK
                     SEYAKHFFNLPSKKPDFNPIVFDRDVETVEIGNGHKLMCVQNPANDIFSLTINFMIGE
                     NTMPLLRLASQGMGLSGAGSYNVTELKSEFARIGASYNIASNDYSVSIELQGVESELD
                     RALELVGMLVGDPSLEQSKIKTIVDGETTARKVERSQPDNVAQALLEYTLYGDSSNYL
                     NRLSSKQLKNLKAEELVSTFKKATEYNTQIYYCGKTGASNVAAEVSKHVPLAANPKHD
                     TNSKDRLAKHYNENTIYLVDKPKARQSKVFVFINGQSFTPADAAVIDAFNDYFGGGFS
                     GLILQEIREYRSLAYSAGGKFKLPRQKGNPADFIGYVGTQSDKTLTAMETFNGLMRQM
                     PEKPERMGLIRSHLELLAQTDRPNFRELASRVDEWRDQGFEADPAFVKLPAYQLLKWQ
                     AIEQFYADRMKGKPAVYMIVGDKKQINLKELSSFGKVIELKEDKLFNK"
     gene            complement(4055..>4299)
                     /gene="rmuC"
                     /locus_tag="PLX53_02090"
     CDS             complement(4055..>4299)
                     /gene="rmuC"
                     /locus_tag="PLX53_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOY71901.1"
                     /translation="QVLGAVKREFENFGGLMEKAQKNIQTGLNQLDDVMGKRTRAIQK
                     KLKSVEALSEAEAKSILPEISESAIVDDEDDDLGEV"
BASE COUNT         1031 a          956 c         1001 g         1311 t
ORIGIN      
        1 tgtttttgat gttgactatc tttcggatga gcctggttga aatcgttgag ggcaagcgtt
       61 tactcccctt atcatccccg ctagcggttt gcctactcag gggtgtttca acatatggtt
      121 catgaaaaaa atcaaattat tggctaattt acctgtttat taatattaat tttctaaatt
      181 tgcgcccaat ttgaagtgta atctcttacc aggacatggg tgccggtaat attgcatttc
      241 ccagttatac ttaaaaatca aactaaaatg gacctcatta aagttgcaga agagtcattt
      301 gcctctaaaa aggagcatcc ttcctttaag agtggtgata ccgttgtagt tcactacaaa
      361 attgttgagg gtagcaagga gcgaattcag aattacaggg gcgtcgtaat tcagagaaga
      421 ggcgtggggg caaccgaaac ctttaccgta agaaagattt caggtggtgt aggtgttgaa
      481 aggatttttc cgcttaactc cccatttatc gaaaaaattg aagtggctaa ggagggaaaa
      541 gttcgcagag cccgaatata ctacctgcgc aacctcacgg gtaaaaaggc acgcattaag
      601 gagaaaaaac gcaagctcga tattggagag taagctgata aaatcccctt taagttgggg
      661 attttttatt aaccacccat gcttaaacca atttaatagc tcccatatcc cttctgaata
      721 tcagtttggg tttaatgcgc aatttgttgt aggcaggcgt ttgcaagtgg ttgcctttat
      781 tgttttgata attgcctgat ttgtagttaa atcagccggt taacgctttg gtaaggagta
      841 aattttactg gttgaaatga cggtttttta ttttttttca gcaggaaatg gttgcatggt
      901 atggaaatat tgaataattt tgcacttgaa acacaaggcc ccgtagctta actgaataga
      961 gcatctgact acggatcaga aggttggggg tttgaatccc ttcggggtca caacaaaaaa
     1021 agaccaatac gcgtattggt ctttttttac ttttcagtaa tctctcccaa agttatttgt
     1081 tgaacagctt atcctccttg agttcaatca cttttccaaa cgatgacagc tccttaaggt
     1141 tgatttgctt tttgtcgcca acaatcatgt aaacagccgg cttacccttc atcctgtcgg
     1201 catagaattg ctctatagcc tgccatttaa gcagctggta ggcaggtagc ttaacaaagg
     1261 cagggtcggc ctcgaaaccc tggtcacgcc actcgtcaac ccttgaagcc agctccctga
     1321 agtttggtcg gtcggtttgc gccaaaagtt caaggtggct tcttataagg cccatacgtt
     1381 cgggcttttc gggcatctgg cgcatcagcc cgttgaatgt ttccatggcc gtcagggttt
     1441 tatcggattg tgttccaacg tagcctataa aatcagctgg atttcctttt tggcggggca
     1501 acttaaattt tcctccggca gagtaggcca acgagcggta ttcccttatt tcctgaagaa
     1561 taagtccaga gaaccctcct ccgaagtagt cgttaaaagc atctattaca gctgcatctg
     1621 caggtgtaaa gctttggccg ttgataaata caaacacctt gctttgtctg gcctttggct
     1681 tatcaaccag gtagatggtg ttttcattgt aatgcttggc cagcctgtcc tttgagttgg
     1741 tatcgtgttt tgggttggca gccaatggga catgcttgct cacctctgcg gctacatttg
     1801 atgcgcctgt ctttccgcag tagtaaattt gagtgttgta ctccgttgct tttttaaagg
     1861 ttgataccag ttcttcggcc ttcaagttct taagctgctt cgacgatagg cggtttaggt
     1921 agtttgaact atcgccataa agggtgtact ccagtagcgc ctgagctaca ttgtcgggct
     1981 gtgaacgttc aacttttcga gcggtagtct ccccgtcaac tattgtttta attttcgact
     2041 gctcaaggct gggatcgcct accagcattc ctacaagctc gagcgctcgg tcaagttcac
     2101 tttccactcc ctgtagttca atagacacgc tgtaatcatt agaggcgatg ttgtacgatg
     2161 cgccaattcg ggcaaactcc gatttcaact cggttacgtt atagctgccg gcgccgctaa
     2221 ggcccattcc ctgtgaggcc aggcgaagca ggggcatggt gttttcccct atcatgaagt
     2281 ttattgttag ggaaaaaata tcgttggcgg gattctgaac gcacatcagc ttatgcccat
     2341 tgcctatttc aactgtttca acatccctat caaatacaat ggggttgaaa tcaggctttt
     2401 tggatggtag gttgaagaaa tgcttagcgt actcgctttt agcttcggtg ttagccaaca
     2461 ggggtttaaa gtctggtttt tctatttttt cctttttggg gaagcccatt ttagagtaga
     2521 aggccagata gctatctccg aagatttggt tagctgcagc aaccacatca tccttggtaa
     2581 gcctcattat tttattaggg tagtcgagcg cttcctgagc ggattgccct ttggcaaaag
     2641 cttctcccag gtagagcgca cgcccatcaa ttttctccat ggtggtaacg tggttgcggt
     2701 agaggtcgag cttaacagcc tccagcatcc agtcgtcaaa ttcgcctcgt ttaagtttac
     2761 caagttcatt attaaccagt gattcagcat cctcaaggct ttggccaagt atctttggaa
     2821 gcataaccac aataacggcg ccatggtcga ggtagggcat ttgcaaagct tgagcggcca
     2881 gcagctttcc atcgagcgtt agcttgtcga gcaatccggt ggagtagctg ttgttgagaa
     2941 tgcgggtagc cacctctgct gccaggaaac catcgtcttt agccgacgga gctctgaatc
     3001 ccagaagccc cattttcacc ggggaaagct tggcctggta gaactccctt ccgttaaatg
     3061 gtttttctgt ccaaatctta ctttctggta gctcatgctc cttccatttg ccaaattttt
     3121 cttcgataac gggaataatc tcgtcggagt tgaaatcgcc ggagagaaca agtgccatgt
     3181 tgttggggac atagtaggtt ttaaaaaaat cgtacatctt gatgagcgag gggttcttca
     3241 aatcctcaat ggagccaatg agcgtttgct gtccgtaggg gtgattctta aagaactgct
     3301 tttggaactc ctccagcagc ttggtgaaaa actggtcgtt gtatagattc ttttcctcgt
     3361 aaaccacctc cagctctgcc tgaaaacccc tgaaaacagg ttcaacaaaa cgatgggcgt
     3421 aaaggtcgag ccagcgttca atctggcttg aagggaacat gttgtagaaa acggtgtagt
     3481 cggggccggt gccggcattg agcatcgaac cgcctatttc gttcaccagg ttgctaaact
     3541 cgttgggaat tgcgaagctg tttgccttga gcgatgcctc gtttatttcc tgctgaattt
     3601 gcttgcgctt agcttcatcg gaagttttac ccagggagtc gtagagttga aaaattttat
     3661 caatgtaagg tttttcctct ttccagttga tggtgccaag ggtttgagtg cccttaaaga
     3721 gcatatgctc ctggtagtgt gccattccgg tggcatcggc agggtcattc ttaccgcccg
     3781 cctttaccat aaccaatcca aaaaccttgg gttgagcgtg attctcgtca agtattacgg
     3841 ttaaaccgtt gttcagcgtg agctgcttta ctctgctatt gtccatttga acctcctgtg
     3901 aaattccagc cagagaagct gcaatcagca ggcttcccat caaaattttt gcttttagca
     3961 tggcttgtta ttttaggttg ttgcattgtt gttgttcaat gttgcaccct ggattatttg
     4021 gtttttttcg aattaaggtt aaataagagc ttagctatac ctcacccaaa tcgtcatcct
     4081 catcatcaac aatagccgat tcggatattt cgggaagtat gcttttggct tcggcctcgc
     4141 ttagcgcctc cacacttttc agttttttct ggatggcgcg ggtgcgcttg cccatcacgt
     4201 cgtccagctg gttcagaccg gtttggatgt tcttttgggc tttctccatc agcccgccga
     4261 aattctcaaa ttccctctta acagcgccca gcacctgcc
//
LOCUS       DAOOQX010000081         4287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_24406_length_4287_cov_3.461011, whole genome shotgun sequence.
ACCESSION   DAOOQX010000081 DAOOQX010000000
VERSION     DAOOQX010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24406_length_4287_cov_3.461011"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PLX53_02095"
     CDS             complement(<1..300)
                     /locus_tag="PLX53_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71902.1"
                     /translation="MKTKLLCFILLLAVIPVLGQVTPQAPQKFSYQAVLRNADGTVIG
                     NQSVEVQVKLHAQAADGAVVYTQTQTVETNQFGLVAFAVGGSSGFETIPWTDPIFM"
     gene            769..2571
                     /gene="typA"
                     /locus_tag="PLX53_02100"
     CDS             769..2571
                     /gene="typA"
                     /locus_tag="PLX53_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOY71903.1"
                     /translation="MQKIRNIAIIAHVDHGKTTLVDKMIFQGKLFKDYERPGELILDS
                     NDLERERGITILAKNLSVNYKGYKINIIDTPGHSDFGGEVERVLNMADGVLLLVDAFE
                     GTMPQTRFVLQKALAIGLKPIVVINKVDKPNCRPAEVQEQVFELMYNLDATEEQLNFP
                     TIYGSAKQGWMSNDWQKPSKDITPLLDCIIDYVPAPKVTEGTTQLLVTSLDYSSYVGR
                     IAIGKLHRGSLQAGQPISLVKRNGEVVRTKIKDLLVFEGLEKNSVERVECGDICALVG
                     IEGFDIGDTIADYENPEPLPTIAIDEPTMSMLFTINNSPFFGRDGKFVTSRHLKERLD
                     RELEKNLALRVEKTDSADSFMVFGRGVLHLSVLIETMRREGYELQVGQPKVIIKMVGG
                     EKCEPIEYLTIDLPEDVSGKAIEQVTRRKGALLLMERRGDRIHLEFEIPSRGIIGLRS
                     ILLTATAGEAVIAHRVKGFEPYRGDFETRTNGSLVAMESGISYAYALNKLQDRGRFFI
                     DDHEEVYAGQVVGEHNRSGDLVVNVTKSKKLTNMRAAGSDEKIFLAPPIRFSLEEALE
                     YIQEDEYVEVTPKAMRLRKIILDETQRKRIARVA"
     gene            2686..3210
                     /locus_tag="PLX53_02105"
     CDS             2686..3210
                     /locus_tag="PLX53_02105"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOY71904.1"
                     /translation="MANVLDAYSINFKGLKLGKHLFSFQLGRSFFSAFEEGEIHNGEL
                     VARVSLNKQSRLLEFSTHIEGMVEIACDRCLEQFNFPVEFDGKLIVKIDASQESDSDE
                     IIYLSENDFEVNLAQYLYESIYLSMPIERYHGMNGTSASDCDSDMVSLLGSHGHENQE
                     VDPRWDKLKSLTSN"
     gene            3244..3429
                     /gene="rpmF"
                     /locus_tag="PLX53_02110"
     CDS             3244..3429
                     /gene="rpmF"
                     /locus_tag="PLX53_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOY71905.1"
                     /translation="MAHPKSKISKQRRNKRRTHYKAELPTLSTCSNCGAAVEYHRVCP
                     ECGYYRGKLAIEKSVSA"
     gene            3462..>4287
                     /locus_tag="PLX53_02115"
     CDS             3462..>4287
                     /locus_tag="PLX53_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HOY71906.1"
                     /translation="MRIGVDAMGGDFAPEAVVRGSILACKQLKADERIVLFGDKAKIE
                     ELINSLEGGSVNCFDIEPCSEVIAMGDNPSKSFSKKTDSSIVRGFSALAAGAIDGFAS
                     AGSTGAMMVGAMLVVKQIPGVIRPAIAGFIPVSDNSVNLLLDVGLNPDCRPDVLYQYG
                     ILGSIYSKQVLGVENPRIALLNVGEEDEKGNLVTKSAFEQMKDSADFNFVGNIEGHNL
                     FDADKADVVVCDGFVGNIVLKEAEAFYHLLKKLSPNSSFTERFNSENYGGTPVLGIN"
BASE COUNT         1197 a          903 c         1065 g         1122 t
ORIGIN      
        1 catgaatatt ggatcggtcc aaggaatggt ttcaaatccg ctgctaccgc caactgcaaa
       61 agctaccagg ccaaactggt tggtctcaac ggtttgagtt tgagtgtaca ccactgcccc
      121 gtcggcagct tgcgcatgaa gcttcacctg cacctcaacg ctttgattgc caataaccgt
      181 accatcggca tttcgcagaa cagcctggta ggaaaatttc tgcggcgctt gaggtgttac
      241 ctgtccaagg acaggaatta cggctaacaa aaggataaag cataaaagtt ttgttttcat
      301 ctgcttaaat ttatttgtct gggtttaatt agggttagga attagccatg agcagaacct
      361 gcctatacca aaaggttact aaaagttata ctcatagctt ctgtttagtg cgttatttgg
      421 taaaatatgt aaggcctgat actaacaaat tgtctatata tatcaagaaa caagaaagta
      481 gaaatcttcc agccacatct ctttttagtg caggttgata cttaaagatt taaaaaaata
      541 caagttacgt tacgctagat taattgtcaa attatgtttc aaaataaaat cttatttttt
      601 ccttttattt atcgatacac ttaaccattc agaaaaaatg aactatttga caaataacca
      661 aaattagtgg gtaaaacttt caaaacaact acatgcctta ctttttatat tcagcgtggg
      721 ggaaaaatta aataaataga attaattttg ccggttaaaa ttttttagat gcagaagata
      781 cgaaacattg cgataattgc gcatgtggat catgggaaga ccacgctggt ggacaaaatg
      841 atttttcagg ggaaactttt taaggattac gaaaggccag gcgagcttat actggacagc
      901 aacgatctgg agcgggagcg gggcatcacc attctggcaa aaaatctttc ggtaaactac
      961 aagggttaca agataaacat cattgatacg cctgggcact ccgattttgg gggtgaggtg
     1021 gagcgggtgc tcaacatggc cgatggggtt ttgctgctgg ttgatgcttt tgagggaacc
     1081 atgccccaaa cccgttttgt gcttcagaaa gcgctggcca tagggcttaa gccaatagtg
     1141 gtgataaaca aggttgacaa acccaactgc cgtccggcag aggtacagga acaggttttt
     1201 gagctgatgt acaaccttga tgccaccgag gagcagctca actttccaac tatttacggg
     1261 agcgccaagc agggatggat gagcaacgac tggcaaaaac cctcgaaaga tattaccccg
     1321 ctgctcgact gcattattga ctacgtgcct gcgccaaaag tgaccgaagg aacaacccag
     1381 cttctggtta catcgcttga ctactctagc tatgtgggcc gaattgccat agggaagcta
     1441 caccggggca gcctgcaggc agggcagcct atatcgctgg ttaagaggaa cggtgaggta
     1501 gttcgaacta aaatcaagga cctgttggtt tttgagggac ttgaaaaaaa tagtgtcgaa
     1561 cgggtggaat gcggcgacat ctgtgctctt gtaggcattg agggctttga tattggcgat
     1621 accattgccg attatgagaa tcctgagcca cttccaacca ttgccattga cgagccaacc
     1681 atgagcatgc tcttcaccat caacaactct cctttctttg gcagggatgg caagtttgta
     1741 acctcccgcc atttgaaaga gcggctcgac agggagctgg aaaaaaatct cgccctaagg
     1801 gttgagaaaa ccgactcggc cgattcattc atggtgtttg gacggggagt gctacaccta
     1861 tcggtgctta ttgagaccat gcgccgggaa ggatacgagc ttcaggttgg tcagccgaag
     1921 gttatcatca aaatggttgg tggcgagaag tgcgaaccca ttgagtacct gactatcgac
     1981 ctaccggagg atgtgtcggg caaggcaata gaacaggtta cccgcaggaa gggtgccctg
     2041 ttgcttatgg agcgacgagg cgacaggatt caccttgagt ttgaaattcc ttcacgtggc
     2101 atcattggac tacgaagcat cctgcttacc gctactgccg gtgaggctgt tattgcccac
     2161 cgggtgaaag ggtttgaacc ctaccggggt gatttcgaga cccgtactaa cggctccctg
     2221 gtggcaatgg agagcggaat ttcatacgcc tatgccctta acaagctaca ggataggggt
     2281 aggtttttca ttgatgacca tgaggaggtt tatgccggac aggtagttgg agagcacaac
     2341 cgctctggcg atttggttgt gaatgttacc aagtcgaaga agctcaccaa catgcgagct
     2401 gccggttcgg acgaaaaaat cttcctggct ccccccattc ggtttagcct tgaggaagca
     2461 ttagaataca ttcaggaaga tgagtacgtt gaggtaaccc cgaaagccat gcggctacgc
     2521 aaaattattc ttgacgaaac ccagcgcaag cgtatcgcca gggttgcata atttctgata
     2581 acccgaactt tccctttagg tgaagccacc aacctaattt tcagccatta tttatacgtt
     2641 tgatatttaa ttttataaat ttgcaaccta attttattgg gtaaggtggc aaacgtgctt
     2701 gatgcatatt caattaattt taagggactt aagctgggaa agcatctgtt tagtttccag
     2761 ctgggaagga gttttttcag cgcatttgag gagggggaaa tccataacgg cgagctggtt
     2821 gccagggtaa gcctcaacaa gcaatcgaga ttacttgagt ttagcaccca cattgagggt
     2881 atggtggaga ttgcctgcga taggtgcctt gaacagttta attttcctgt tgaatttgac
     2941 ggcaagctaa ttgtaaagat tgacgccagc caggaatcag attccgatga gattatctac
     3001 ctttccgaaa atgactttga ggttaacctg gcacaatatc tttacgagag tatatacctg
     3061 agtatgccaa tagaacgata ccacgggatg aacggaacaa gcgcttccga ctgcgatagc
     3121 gatatggtat cattgcttgg ttctcatggg cacgaaaatc aggaggttga tcctcgttgg
     3181 gacaagctca aaagcttaac aagtaactaa gtatcaacaa cagtaactta aatatttcac
     3241 aaaatggcac atcctaaatc aaaaatttcc aaacagagga ggaataagcg tagaacccac
     3301 tacaaggcag aattgccaac tctttctact tgttcaaact gtggtgcagc tgtggagtac
     3361 cacagggtat gcccggagtg tggatactac cgtggcaagc tggcaattga aaaatctgtc
     3421 agcgcctagt agtcatctcg tattcaacca aaaccacaac gatgagaatt ggcgttgatg
     3481 cgatgggcgg tgattttgcc cccgaagctg ttgtaagggg ttctattcta gcctgcaaac
     3541 agcttaaggc tgatgaaagg attgttttgt ttggcgacaa ggcaaaaatt gaggagctga
     3601 ttaattcgct ggagggtggt agcgtcaact gctttgatat agagccgtgc tccgaggtta
     3661 ttgccatggg cgataatcca tcaaaatctt tttctaaaaa gaccgattcc agcatcgtga
     3721 gagggttctc ggctcttgct gcaggtgcaa tcgatggttt tgcaagtgcc ggcagcaccg
     3781 gcgccatgat ggtcggcgcc atgcttgtgg ttaaacaaat ccccggtgtt attcgtcccg
     3841 ctattgccgg gtttatcccc gtttcggaca acagcgtaaa cctgttgctt gatgtgggac
     3901 tgaaccctga ctgccggcct gatgtccttt accagtacgg tatccttggg tcaatttact
     3961 ccaagcaggt gctgggtgtc gaaaatccac gcattgcact ccttaacgta ggagaggagg
     4021 atgaaaaggg taacctggtt actaaaagtg ctttcgagca aatgaaggat tcggcggatt
     4081 tcaactttgt tggcaacatt gaggggcata acctcttcga tgccgataag gccgatgtgg
     4141 ttgtttgcga tgggtttgtt ggaaatatag tgctaaaaga agccgaggct ttttaccatc
     4201 ttttaaaaaa actcagcccc aacagctcat ttaccgagcg cttcaactca gagaattacg
     4261 gtggcacacc tgttctggga attaaca
//
LOCUS       DAOOQX010000082         4256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_24657_length_4256_cov_2.760533, whole genome shotgun sequence.
ACCESSION   DAOOQX010000082 DAOOQX010000000
VERSION     DAOOQX010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24657_length_4256_cov_2.760533"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..2049)
                     /gene="ispG"
                     /locus_tag="PLX53_02120"
     CDS             complement(160..2049)
                     /gene="ispG"
                     /locus_tag="PLX53_02120"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOY71907.1"
                     /translation="MNYCKNLYSYSRRETQEVNIGGVPIGGSNPIRIQTMTTTNTNDI
                     LATVEQCIKAAKAGADYVRITTQGVKEATSLSSIKQELLNRGFTTPIIADIHFNPNAA
                     MEAAKYADKVRINPGNFVTSHAKPTGGSGKQQEEEDNTALIEAKLVPLLELCRERGVA
                     LRIGVNHGSLSERIMAKYGDTPEGMVESALEFIKICARHNFRDIVVSMKASNVRVMVQ
                     AVRLLAARMLNEGMEYPIHLGVTEAGNGEDGRIKSAVGIGTLLADGIGDTVRVSLTED
                     PENEIPVAKKLVSYFENRSSFGRIPSMDPLPYNPFSYTRRKTIAVGSIGADNPPVVVA
                     NLSLATKITGKLLSAWGWTYNHAANQWEHSELAADYFYIGSTTIDEEVITDGLPLLST
                     ANSSSQILRQGNKPASFNISAQPICQMVTDADVLSGHLFSTVMVSPNTVLILRTGYSN
                     GYAGQRAAFIRLMNNHENIPVIISREYDDDDRESFQLKASADIGGLLVDGLGDGVMVT
                     APAIADEDICSTVFSVLQAARTRITKTDYIACPGCGRTLFNLQQTLEKVKAHTGHLRG
                     LKIAVMGCIVNGPGEMADADYGYVGAGPGRVTLYKGKQVMKKNIPEEDALDELIGLIN
                     ADRGL"
     gene            complement(2517..3017)
                     /locus_tag="PLX53_02125"
     CDS             complement(2517..3017)
                     /locus_tag="PLX53_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71908.1"
                     /translation="MNTGIAIIGVLIVVISIIPFILVSRAKRKKEKRLRSEIAGMAKS
                     HGCSISMLDVLKDYAVGADENKHFIFFYKHQNGNETKLAINLADIQSCRVINSTRTIG
                     GRDSQYVITDKVALCLTPVEKSNPEIHLELYSADDGTQLVGELELVEKWSKAINNQLK
                     SMKPIK"
     gene            complement(3133..3495)
                     /locus_tag="PLX53_02130"
     CDS             complement(3133..3495)
                     /locus_tag="PLX53_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71909.1"
                     /translation="MKREVRIVGTIFLVALYCFAASVVCIPKLVPLEEPHNGPGYFFN
                     ASSNLLSLTLPSKNPAGSIGLSPIWVLKNQLKDFSYTIKSVELFLSTIFTQYRNVSIS
                     YPIRLRKTDLLYPFQYFW"
     gene            complement(3620..>4256)
                     /locus_tag="PLX53_02135"
     CDS             complement(3620..>4256)
                     /locus_tag="PLX53_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOY71910.1"
                     /translation="LAKENDVELIDATCPVVLKLQERVRKAWEQMKIAGGTVIIYGKK
                     GHAEVIGLVGQTDGNAVVVESPNDLTGIDFSKPVEIFSQTTKDAEMFKQIVDVIKEKM
                     QSCFLSSNIPIKVNHTFCGQVANRKKELIGFAATHDVIIFVGGQTSSNGKMLFQTCRS
                     SNPKSYYVSSANELRPEWFAGANSVGVSGATSTPLWLMEQVAQEIERITMP"
BASE COUNT         1095 a          944 c          967 g         1250 t
ORIGIN      
        1 accccattca cggttgcttt tcccatgata tttggcaatt tcaacagctt acttaggctt
       61 atagcttctc gtacaaagcc gtacaaaacc cgaatgggct taaacaagaa aatctggcca
      121 tttgtgagtt gccctggggt tgagtaaagc cgaaaaaagt tacaaacccc tgtcggcatt
      181 aatcaggccg attagctcat caagggcatc ctcctcgggt atatttttct tcataacctg
      241 tttacccttg tagagtgtaa ctctaccggg accagctcca acatatccat agtcggcatc
      301 ggccatttcg cccggtccat tcactatgca gcccattact gcaattttaa gtcccctaag
      361 gtggccggtg tgagccttta ctttttcgag cgtttgctgc aggttgaaga gagttcgtcc
      421 acaaccaggg caggctatat aatcggtttt ggttattcgt gtacgggcag cttgaagcac
      481 ggaaaaaacg gttgagcaaa tatcctcatc ggcaattgcc ggtgcggtaa ccataacccc
      541 atcgcccaac ccatcaacca gcagtccgcc gatatctgct gaggctttaa gttgaaagga
      601 ttccctatcg tcatcatcgt actcccgact aattatcacc ggaatgttct catggttgtt
      661 cattaacctt atgaaagctg ccctttgtcc tgcataacca ttggagtagc cagttctcag
      721 aattaataca gtattgggac taaccataac agtggagaat aggtggcctg aaagcacatc
      781 ggcatcggta accatttggc atatgggttg cgccgaaatg ttaaaggagg cgggtttgtt
      841 tccctgacga agtatttggc tactagagtt ggcagtggaa agtaacggta agccatcagt
      901 aatgacttct tcgtcaatgg ttgttgagcc aatgtagaag tagtcggcag caagttctga
      961 atgttcccat tggtttgctg catggttgta tgtccatccc catgccgata aaagtttacc
     1021 ggttattttt gtggctaacg agaggttggc cactacaacg ggaggattat cggccccgat
     1081 gctaccaaca gctatggttt tccttcgggt gtagctgaat gggttgtagg ggagggggtc
     1141 catgctggga attctgccaa aggaggatcg attttcgaag taggaaacaa gctttttagc
     1201 cacgggaatc tcgttttcag gatcctcggt aagcgatact ctaacggtgt cgccaattcc
     1261 atcggcaaga agggtcccaa tgccaacagc cgatttaata cggccatctt caccattacc
     1321 ggcctcggtt acgccaaggt gaatggggta ctccatcccc tcgttgagca tcctggctgc
     1381 cagtaacctt acggcctgaa ccatgaccct cacgttgctt gccttcatgg aaaccactat
     1441 gtctcgaaaa ttgtgtctgg cgcatatttt gatgaactcc aaggctgatt ccaccattcc
     1501 ttcaggggta tcaccgtact tggccataat tctttcggat agagaaccat ggttgacccc
     1561 tattcttagt gcaacacctc gttctcggca aagctccaac aacgggacca gcttagcttc
     1621 tatcaaggca gtattgtctt cttcttcttg ctgtttacca ctccctccag ttggctttgc
     1681 atgggaagta acaaagttac ccgggtttat cctaaccttg tcggcatact tggccgcctc
     1741 cattgccgca tttgggttga agtgtatatc ggcaattata ggggttgtga aaccacggtt
     1801 tagcagttcc tgctttatgc tgctaaggct ggtggcttcc ttaaccccct gagttgtaat
     1861 cctgacataa tcagcgcctg ccttggcggc tttaatgcac tgctcaacgg ttgccaagat
     1921 gtcgttggtg ttggtggtgg tcatggtttg aatacgaatg ggattcgaac cacctattgg
     1981 caccccgcca atattaactt cctgagtttc gcgcctggag taactataga gatttttaca
     2041 gtagttcatt tgcttctatc tggctgttgt ttttcgttta caaattaaca aacttcggta
     2101 acaaatggct tatgcaagta agtaaaatcc gaatattttt ttactgtgaa aatttattcc
     2161 agtttcatat ttgcataatt ccgtagttcg tataatcggc acgtaaccag ccaaggtgca
     2221 tgctgattta agattctatt gaaaatggtt aggtaaagaa accaataccc acaatgggct
     2281 aaatatcgca agtctagtgg tgccatagaa aaatgtccat cctaattgca tgattttcaa
     2341 cggataatta caagtagatt gatgagtttt gagaaaataa ccctaacgct gattcagtaa
     2401 acattagata agctcatttc aaggtcaatt taaaaagtgg ttgaggtagg tcgaaaaaag
     2461 cccttcaatc gtgcatgaag ggcttttcaa tggtaaggtg taaagaagtt attggtttac
     2521 ttgatgggtt tcatgctttt aagctggttg ttgatagctt tcgaccattt ttcaaccagc
     2581 tccagctctc ctaccagctg ggtgccgtcg tcggcactat agagttcaag atgaatctcg
     2641 gggttggatt tctccaccgg cgtaagacat aaagccacct tgtcggtaat gacgtactgg
     2701 ctgtcccttc caccaattgt gcgcgttgaa tttattaccc ggcagctctg aatgtcggcc
     2761 aggttaatag ccagcttggt ttcatttcca ttctggtgct tgtagaagaa tatgaaatgc
     2821 ttgttttcgt cagctccaac agcatagtct ttcagaacat ctagcatgga aatgctgcag
     2881 ccatggcttt tggccatccc ggcaatctca ctgcgcagcc ttttttcctt tttccttttc
     2941 gccctgctta cgagaataaa tggtatgatg ctgataacca ctattaaaac acctataata
     3001 gctattcctg tattcatttt tgtagattta gataagttga aaaatagtta ttcaggtttt
     3061 gttgaggcac aatacctgca aaaaaccttt aggagttgcc gaaagcggca atgtggttaa
     3121 gttggaatgc gcttaccaga agtattgaaa tgggtaaagc agatcggttt ttcggagcct
     3181 tattgggtag ctaatggaaa catttcggta ctgtgtaaat atggtggaca ggaacagctc
     3241 aacggatttt atagtatagg aaaagtcttt tagctgattt tttaaaaccc agatggggct
     3301 aaggccaatg ctgcctgccg gattttttga aggtagagtt agggaaagca gatttgatga
     3361 ggcgttaaaa aagtagcctg gcccattatg tggctcttca aggggaacaa gtttagggat
     3421 gcaaaccaca cttgctgcaa agcagtaaag agcaacaaga aaaatggtgc cgactatgcg
     3481 aacttccctt ttcatgttgc aaacgtaagc aaaaaaatta ttgccagagc atcataataa
     3541 gctcttttgt aggttattga gcggaaaatt atctgccata tgatttactt gaaggaatgg
     3601 taggcagaat atgaggaatt tagggcatgg tgatgcgttc aatttcctgt gccacctgct
     3661 ccatcagcca taagggagta gatgttgcac cgctaacgcc cacgctgttt gctccagcaa
     3721 accattcggg ccgaagctcg ttggcggagg aaacatagta tgattttgga ttgcttgacc
     3781 tacaggtttg aaaaagcatt ttaccattgg agctcgtttg acctccaaca aagatgataa
     3841 catcgtgagt tgcggcaaat ccgattagct ccttttttcg gttggctacc tgtccgcaaa
     3901 acgtatggtt aacctttatg ggtatgtttg aggacagaaa gcaggattgc attttttcct
     3961 taattacgtc cactatctgc ttaaacattt ccgcatcctt agtggtctgc gagaatatct
     4021 ctaccggttt ggagaaatca attccggtta aatcgtttgg cgattccacc actacggcat
     4081 tcccatcggt ttggcccact agcccgataa cctcggcatg gccttttttc ccatagataa
     4141 ttaccgtacc gccggctatt ttcatttgct cccacgcttt ccttacacgt tcctgcagct
     4201 tgagaacaac cggacaggta gcatcaatga gctcaacgtc gttttctttt gccagc
//
LOCUS       DAOOQX010000083         4241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_24772_length_4241_cov_3.321070, whole genome shotgun sequence.
ACCESSION   DAOOQX010000083 DAOOQX010000000
VERSION     DAOOQX010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24772_length_4241_cov_3.321070"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PLX53_02140"
     CDS             complement(<1..324)
                     /locus_tag="PLX53_02140"
                     /inference="COORDINATES: protein motif:HMM:NF025807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71911.1"
                     /translation="MVKKFSKHIELRFYGGLNHFLPASKRQVPFNLPYMGTPTVKDLI
                     ESVGVPHVEVFLIVADSISVGFDYKPEPSSQISCYPRFYNLSIDYRVLRPPEPSPPVF
                     ILDVHL"
     gene            460..978
                     /locus_tag="PLX53_02145"
     CDS             460..978
                     /locus_tag="PLX53_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOY71912.1"
                     /translation="MAIIKPLRGFTPKIGENCFIAETAVIIGDVEIGDDCSIWYGVVV
                     RGDVNSIRIGNRVNIQDNAVLHTTYQKTTIRLGNDVSVGHGAIVHGAEVRDGALLGMG
                     CTVMDNAVIGEGAIVAANALVLENTTVEPGTIYGGVPAKFIKKVDAEVSREMNERIAR
                     NYLFYAGWYKQG"
     gene            1135..3189
                     /gene="metG"
                     /locus_tag="PLX53_02150"
     CDS             1135..3189
                     /gene="metG"
                     /locus_tag="PLX53_02150"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOY71913.1"
                     /translation="MYKPFHPMSNYKRYLITSALPYANGPVHIGHLAGVYIPSDIYTR
                     YLRAKGADVISVCGSDEHGVPITIKARHENTTPQAVVDKYHAIIKESFKQFGIDFDIY
                     SRTTSPVHYETASEFFKKLYEKGVFVEKVTDQYYDEEVGQFLADRYITGTCPHCGNPN
                     AYGDQCEKCGTSLNATDLIDPRSTLSGSKPILKATRHWFLPLDKFQPELEKWILEDHK
                     EWKANVYGQCKSWLDQGLQPRAVSRDLDWGVPVPLDGAEGKVLYVWFDAPIGYISATK
                     ELTPNWEKYWKDSDTRLIHFIGKDNIVFHCIIFPSMLKAYGDYILPDNVPANEFLNLE
                     GDKISTSRNWAVWLNEYLVEFPGKQDVLRYVLTANMPETKDNDFTWKDFQTRNNSELV
                     AILGNFVNRAMVLTHKYFDGKLPARNTLTDYDNSVLHELPRIKERLEHNLEEFKFREA
                     LKEAMNIARLGNKYLADTEPWKLMEKDPRQVANILNIAMQITANLSIILEPFLPFTAE
                     KIRKMINFQSKEWKLAGLDSLLAPGHILDKPQLLFDKIEDDVIAVQLNKLQATKLVNQ
                     NSVKNVPEQKGSISYDDFSKMDIRVATVLDAERVPKTQKLLKLKVDTGIDVRTLVSGI
                     AEYFTPEEMVGRQVLVLANLEPRKIRGIDSHGMILMAEDTSGKLQLVSPAAKVSNGST
                     VK"
     gene            complement(3272..>4241)
                     /locus_tag="PLX53_02155"
     CDS             complement(3272..>4241)
                     /locus_tag="PLX53_02155"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOY71914.1"
                     /translation="IFDTSRTFDDEAITNYLCNTLGIDHKEAELRVTQLVERVNNALS
                     SRKPFEFPGVGTLLKDDNNNITLEFDASAAATESYGLSAIEVLPKQKADSKLTSPKEN
                     KNAAVAETQASSPLSTEQTTKIPTPKPAVAKKSRVRLWVPITACVVVVAVAVCWFTPA
                     LHFWEQSQTSVSPSTTDSTSSMASVGNLPLASAIPDSIVEEEDSQVPDSISASGQPNA
                     TDEGGAEKILEQTTSKKVALSYTEPPVVETGKSFYIIAGSFTSEVNAQKFLSELAEKG
                     YKPTLIPRNKLYSVSLYTFSNRDRALRELERLKSEQTFEKLWLLSQ"
BASE COUNT         1128 a          911 c         1008 g         1194 t
ORIGIN      
        1 caggtgaacg tcgaggatga atacaggtgg cgagggttct ggcggccgca gcaccctgta
       61 atctatgcta aggttgtaaa atcgcgggta gcatgaaatt tgggagctgg gttcaggctt
      121 gtagtcaaaa ccaaccgata tgctgtcagc cacaattaaa aatacctcta catgcggtac
      181 tccaaccgat tcgataaggt ccttaactgt gggtgttccc atgtagggta ggttaaatgg
      241 aacctgccgt ttcgacgccg gtaaaaaatg gtttaaaccg ccatagaagc gcagctcgat
      301 atgctttgag aattttttta ccattttaaa tttattcggt gcaacctggc agattttaat
      361 ggatttgtaa accaatttcc ccattccatc ctgttaaaca ggtgaatttt tacaacaata
      421 gttttcattg ctatttttgt gaaaaaaata cactaaagta tggcaataat caaaccactt
      481 aggggattta ctccaaaaat tggagaaaac tgttttattg ccgaaacggc agtaatcata
      541 ggagatgtag aaattggcga tgactgtagc atttggtatg gcgttgtggt tagaggggat
      601 gtgaactcca tccggattgg caaccgggtt aacattcagg ataacgcggt tttgcatacc
      661 acctaccaaa aaacaacaat caggctgggc aacgatgtat cggttggcca tggtgcaatt
      721 gttcatggtg ccgaagttcg cgatggtgcg ctgctaggaa tgggatgcac ggttatggat
      781 aatgcggtta ttggcgaggg tgcaattgtt gctgctaatg cgctggttct ggaaaatact
      841 actgttgaac cggggacaat ctacggaggt gttccagcca agtttatcaa aaaggttgat
      901 gcggaggtta gccgtgagat gaacgagcga attgcccgta actacctgtt ttatgcagga
      961 tggtataagc aaggataaag gattcaaaaa cttgtttgcc taccggcgga acttgtccgg
     1021 ctccggctgt atcaatttaa attcccttat tcttctttct tcttgccgcc tagcggtaac
     1081 aactttactt ttcccgctct acgtttggcc ataaatatgt agatttgcaa caatttgtat
     1141 aaaccttttc acccaatgag taactataaa agatatctta ttacttctgc gttgccctat
     1201 gccaatgggc ctgtgcatat tggacatctg gcgggtgttt acatcccttc cgatatttac
     1261 acccgctatc ttagagcaaa aggcgcagat gttatatctg tttgcggctc cgatgagcat
     1321 ggggttccca taaccatcaa ggccaggcat gagaatacaa ctccacaggc tgttgtggat
     1381 aagtaccatg ccataataaa agaatcattc aaacagttcg gaatcgattt cgatatatac
     1441 tcccgcacca cttcgcctgt tcactacgaa actgccagcg aattttttaa gaagctctac
     1501 gaaaagggtg tttttgttga aaaggttacc gatcagtact acgatgagga ggtgggtcaa
     1561 tttctggccg acaggtatat taccggaact tgcccgcatt gtgggaatcc caacgcttat
     1621 ggtgatcagt gcgaaaagtg cggaactagc cttaatgcta ccgatctgat tgacccaagg
     1681 tcaaccctaa gcggttccaa gcctatactg aaagcaacca ggcactggtt tttgccgcta
     1741 gacaaatttc aacccgagct tgaaaagtgg atacttgaag accacaagga gtggaaagcc
     1801 aatgtttacg gacagtgtaa aagttggctc gaccagggtt tacaaccccg ggcggtaagt
     1861 cgcgacctcg attggggtgt tccggttccg ctggatggtg ccgagggaaa ggtgctttac
     1921 gtttggttcg atgctcctat tggctatata tcggcaacca aggagttgac cccaaactgg
     1981 gagaaatact ggaaggatag cgatacccgt ttaatccatt ttatcggtaa ggataacatt
     2041 gtttttcact gcattatttt cccctccatg cttaaagcat acggagatta cattctaccc
     2101 gataatgtcc cggccaatga gtttcttaac ctggaaggcg ataaaatttc cacctctcgc
     2161 aactgggctg tttggctgaa cgagtacctg gttgagttcc ccggcaagca ggatgtgctg
     2221 aggtatgtac ttaccgccaa catgcccgag accaaggata acgatttcac atggaaagat
     2281 tttcaaaccc gtaacaacag cgagctggtt gccatcctgg gaaattttgt taacagggct
     2341 atggttctta cccataagta cttcgatggc aaactgcctg ccagaaacac tcttacggat
     2401 tacgataatt cagttttgca cgaattgccc cgtataaagg aaaggttgga gcacaacctt
     2461 gaagagttta aatttcgtga agcgcttaaa gaagccatga atattgcccg tttgggcaac
     2521 aagtatctag ctgatactga accatggaag cttatggaga aggatccccg gcaggttgcc
     2581 aacattctta acattgccat gcagattaca gcgaacctct ccattatact tgaacctttt
     2641 ctgccattta ctgcagagaa gatacgcaag atgattaatt ttcagagtaa ggaatggaag
     2701 cttgccggtt tagatagcct gctggccccg gggcatattc ttgacaaacc ccagctgcta
     2761 ttcgataaaa ttgaggatga tgttattgct gtccagctaa ataagcttca agccaccaag
     2821 ctggtaaacc agaattctgt taaaaatgtt ccggaacaaa agggttctat aagctacgac
     2881 gatttttcta aaatggatat tcgggtggca accgttctcg atgctgaaag agttcctaaa
     2941 actcaaaagc tgcttaaact taaagttgat acgggaattg acgtacgcac cttggtttcc
     3001 ggaattgccg agtacttcac ccccgaggag atggttggac gacaagtgct agttttagca
     3061 aatcttgaac cgcgaaaaat taggggaata gattcgcacg gcatgattct gatggcggag
     3121 gatacaagtg gaaagctaca gctggtttcg ccggcagcca aggtttctaa tggctcaact
     3181 gtaaagtaga aatactattc tttaggaata ggtgctggct actttttgtt gtttagtagt
     3241 agcgcctatt ttcttacaac cggttcaccc tctactgact taaaagccat aatttttcaa
     3301 atgtttgctc tgattttaat ctttccagct cccgcaaagc cctgtcgcgg ttgctaaaag
     3361 tatagagcga aacgctgtag agcttgttac gtggtataag ggttggtttg tagccctttt
     3421 ctgccagctc actaaggaac ttctgggcat taacctcgct tgtaaagctg ccggcaatta
     3481 tgtagaaaga tttacccgtt tcaaccaccg gtggctctgt gtagctaagc gctactttct
     3541 tactggtggt ttgttccaga atcttttcgg ctccaccctc atcggtggca ttaggttggc
     3601 ctgaggccga tatgctatcg ggcacttgcg agtcctcttc ttctacgatg gaatccggaa
     3661 tggcagaagc taaaggaagg ttacctacgg atgccatgct ggacgtgcta tcggttgttg
     3721 atggagaaac cgaggtttgc gattgctccc aaaaatgcaa tgctggagta aaccaacaca
     3781 ctgctactgc taccaccact acgcaggctg taatggggac ccataaccta acccgcgatt
     3841 ttttagcaac agctggcttt ggagtcggaa ttttggtggt ttgctctgtt gatagtggac
     3901 tggacgcctg ggtttcggct acagctgcat ttttgttttc cttcggactg gttagtttgg
     3961 agtctgcttt ttgcttaggg agcacttcta ttgctgaaag tccatacgac tcggttgcag
     4021 ctgcgctggc atcaaattca agagtgatat tgttgttgtc atcctttaac aaagttccaa
     4081 caccggggaa ctcgaaaggt tttctactgc taagtgcatt gtttaccctc tccacaagtt
     4141 gcgttaccct taattctgct tccttatgat caattcccag tgtgttgcac aggtagtttg
     4201 taatagcctc gtcgtcaaac gtgcgcgatg tgtcaaaaat g
//
LOCUS       DAOOQX010000084         4211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_24991_length_4211_cov_3.644610, whole genome shotgun sequence.
ACCESSION   DAOOQX010000084 DAOOQX010000000
VERSION     DAOOQX010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24991_length_4211_cov_3.644610"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2351
                     /locus_tag="PLX53_02160"
     CDS             <1..2351
                     /locus_tag="PLX53_02160"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOY71915.1"
                     /translation="QTREPIAYVNIAFRGLPIGTITSEKGEFFLETRTTGDSLTFSYV
                     GYQSKTVAVKKGYFQEINVELQPEAIELASIVVKPGEYQANRIVKNIIRNKDKNNPAN
                     LSYFCHSYNKIQVDLNNVNLEASQRKFLNQFKFIWDYVDTNAITGKPYLPIFITESLS
                     DYYYRSSPKMEKEIIKATKISGVNNESVAQFTGMIYQNVNIYDNYINIFDQGLVSPIA
                     NAGLIYYKYILVDSAFVGNRWCYQISFKPRRKMEPTFTGDFWVNDTTWAVVKAQFRLS
                     DMVNLNFVNDMVTTSEYVPINDTLWFPKQLTMFVDFNLTDKEAGFFGHKTVTYSDVTL
                     NPDFPSEITEQPTSLKVMEGALNQDKEYWNKTRPFELTPKEANIYAMVDSVQQVPLYK
                     TFVDIINMIVNYYYVVGYVEIGPYYQTYSFNEIEGNRFKVSGRTSNEFSTKVMLSGFL
                     AYGDKDNRFKWGVGSMYMIGKNPRQVLSLDYKSDIEQMGQSPNALTEDNILTSFLRRN
                     PNYKLTMVKDFSVSYEKEWFTGLSNTIYFSNRIMYPTEYIPFLPVGGGLQLKNITTSA
                     VTLNTRWVKDETFVQGEFTRVSMGSKWPEVNFDITKGIKGVLGSDYSYWKLHLNYYHK
                     FNVNPFGYARVIADAGKTFGEVPYPLLQLHEGNETYAFDRYAFNMMNYYEFASDQYAS
                     LYLEHHFQGFFLNRFPLLRRLKLREVATAKGLIGSISNSNMNVLQFPEGLGSVSKPYV
                     EVGVGVENILKVIRIDAIWRLTHLDNPDISPFGIRVGLQIIF"
     gene            2400..3125
                     /gene="lptB"
                     /locus_tag="PLX53_02165"
     CDS             2400..3125
                     /gene="lptB"
                     /locus_tag="PLX53_02165"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOY71916.1"
                     /translation="MRLFTENLVKRYKTRTVVKGVSVEVSQGEIVGLLGPNGAGKTTT
                     FYMIVGLVKPNEGKIFVDDVEITGEPVYKRAQRGISYLAQEASVFRHLSVEDNLKAVL
                     EMTKFSKEYQMEKVESLLTEFGLQKIRKSKGIQLSGGERRRTEIARALALSPKFILLD
                     EPFAGVDPIAVEDIQNIVAHLKDKNIGILITDHNVHETLSITDRAYLLYDGEILKSGT
                     SEELADDEQVRKLYLGQNFELRK"
     gene            3192..3273
                     /locus_tag="PLX53_02170"
     tRNA            3192..3273
                     /locus_tag="PLX53_02170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3226..3228,aa:Leu,seq:tag)
     gene            3505..3978
                     /gene="coaD"
                     /locus_tag="PLX53_02175"
     CDS             3505..3978
                     /gene="coaD"
                     /locus_tag="PLX53_02175"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOY71917.1"
                     /translation="MSKVALFPGSFDPITVGHESLVLRALSIFDRIVVAIGNNSSKKS
                     YFDIDLRKAMIEKVFANEPRVEIICYDGLTVDICNKLNINHILRGLRTAADFEFERAI
                     AQVNRAMYPHIDTIFLLTAPEHTFVNSSVIRDILQHHGDATKFIPASINIYDYMG"
BASE COUNT         1154 a          955 c          965 g         1137 t
ORIGIN      
        1 ctcaaaccag ggagcctata gcctacgtga acattgcttt taggggactt cccattggaa
       61 ctattacctc cgaaaaaggt gagtttttcc tcgaaacaag aactacgggc gactcactta
      121 ccttttctta tgttgggtac cagtcgaaaa ccgtagcggt taaaaagggg tattttcagg
      181 agattaacgt ggaacttcag cccgaggcta ttgagcttgc cagcattgta gtaaaaccag
      241 gcgaatatca ggcaaaccgt attgtgaaaa acattatcag gaataaggat aaaaacaacc
      301 cggccaacct cagctacttc tgccatagct acaataaaat tcaggttgac ctgaacaatg
      361 taaaccttga agcttcgcaa cgtaagtttt tgaatcagtt taaatttatt tgggactacg
      421 tggacaccaa tgccataacc ggaaaaccct accttccgat tttcatcacc gagtcgcttt
      481 ccgactacta ctaccgttca agcccaaaaa tggagaagga aattattaag gctaccaaaa
      541 tttcaggtgt taacaacgag agcgttgcgc agtttacggg tatgatatac cagaacgtga
      601 acatttacga taactacatc aatattttcg atcagggttt ggttagcccg atagccaatg
      661 ccggcttaat ctactacaag tacattctgg ttgatagcgc ctttgtggga aatcgctggt
      721 gctaccagat ttcgtttaag ccccgtcgga aaatggagcc tacatttacc ggcgattttt
      781 gggtgaacga caccacctgg gctgtggtga aagcccagtt ccgcctttcc gatatggtta
      841 accttaactt tgtaaacgac atggttacca cctcggagta cgtcccaatt aacgataccc
      901 tctggttccc gaagcagcta accatgtttg ttgattttaa ccttaccgat aaggaggccg
      961 gtttttttgg gcacaaaaca gttacctaca gcgatgttac ccttaatccc gacttcccaa
     1021 gcgagattac cgagcagcca accagcctta aggttatgga gggtgcgcta aatcaggata
     1081 aagagtactg gaataagacg cgaccttttg agctgacgcc caaggaggcc aacatttacg
     1141 ccatggtgga ctcggtgcag caggttcctc tatacaaaac ttttgttgac ataatcaaca
     1201 tgattgtgaa ctactactac gttgtggggt acgttgagat tggcccctac taccaaacat
     1261 acagcttcaa cgaaattgag ggtaaccgct ttaaggtgtc gggacgaaca agtaacgagt
     1321 ttagcactaa agttatgcta tcgggttttc tggcctatgg cgacaaggac aaccgattca
     1381 agtggggtgt tggttccatg tacatgatag gaaagaatcc gcgccaggtg ttaagccttg
     1441 actacaaaag cgacatcgag caaatgggac aaagcccaaa tgcccttacc gaggacaaca
     1501 tcctcacctc gtttcttcgc cgaaacccta actacaagct aaccatggtg aaggatttta
     1561 gcgtgtcgta tgaaaaggag tggttcaccg gattgtccaa tacaatttat ttcagcaaca
     1621 ggataatgta ccccacggag tacatcccat ttctcccggt tggcggaggt ttgcagctga
     1681 aaaatatcac cacctcggca gtaaccctta acacccgctg ggtaaaagat gaaacttttg
     1741 ttcagggtga attcacccgg gtaagcatgg gcagtaaatg gcctgaggtg aacttcgaca
     1801 ttaccaaggg tataaaaggc gtgctgggta gcgattacag ctactggaag ctgcacctga
     1861 actactacca caagtttaac gttaatcctt ttggctacgc ccgggttatt gccgatgccg
     1921 gtaaaacatt tggagaagta ccctacccgt tacttcagct tcacgagggt aacgaaacct
     1981 atgctttcga caggtatgca ttcaacatga tgaactacta cgaatttgcc tccgatcagt
     2041 atgccagctt gtaccttgag caccacttcc agggtttttt cctgaaccga tttcccctgt
     2101 tgcgcaggct taagcttcgc gaggtggcca ccgccaaggg actcatcggc tccatcagca
     2161 attccaacat gaatgtgctt cagtttccag aggggctggg ctccgtatca aaaccctacg
     2221 ttgaggttgg ggtgggagtg gagaatattc tcaaggttat taggattgat gccatttggc
     2281 gccttaccca tctggataat cctgatattt cgccttttgg aatacgggtg gggctacaaa
     2341 ttattttctg agtagcaaca atttgtttac ttttggagct taaataatgc aagctagcta
     2401 tgcgtttatt tactgaaaac ctggtaaaac ggtataaaac ccgaactgtt gttaagggcg
     2461 taagcgttga ggtgagccag ggtgaaattg tcgggctgct tgggcccaat ggcgccggaa
     2521 aaacaacaac cttctacatg attgttgggc tggttaaacc caacgagggg aaaatttttg
     2581 ttgatgatgt tgaaatcacc ggcgaaccgg tttacaagcg cgcccagcgc ggtataagct
     2641 acctggccca ggaggcttcg gttttcaggc accttagcgt ggaggataac ctaaaagctg
     2701 ttctggagat gaccaagttc agcaaggagt accagatgga aaaggtggag tcgcttctca
     2761 ctgaatttgg gctgcagaaa ataagaaaga gtaagggaat tcaactttcc ggcggtgaac
     2821 ggagaagaac cgaaattgca agggcactgg cgctcagtcc taagtttatt cttctcgacg
     2881 agccatttgc cggggttgac cctattgccg tagaggatat tcagaatatt gttgcccacc
     2941 ttaaggataa gaacatcggt attcttatta ccgatcacaa cgttcatgaa acgctttcaa
     3001 ttaccgacag agcatatttg ctatacgacg gggaaatact taaatcggga acatccgagg
     3061 agctggccga cgacgaacaa gttcgaaagc tctaccttgg ccagaacttc gagcttcgta
     3121 aataattttt ccttttaagg ctactcccgg ttggctgctt aaaaaaatca gctaactttg
     3181 ccaaaaatta tgcggatatg gcgtaattgg tagccgcgct agacttagga tctagtgccg
     3241 taaggcgtgt gggttcgagt ccctctatcc gcacttgacc cggaaaagtt ccgggttttt
     3301 tgttttgcta ccagccttaa tagttcctct cccctcaaat ccgctatttg tagtcataat
     3361 agcccttaac tccattgcca ttgtgggggg attggtggtg aaggtgtaag caattcattt
     3421 tcccgattta ctggcattca aaatttttac ctactattgt acgattaaag ttttgctact
     3481 acttacagct aaatctgcca tcacatgagc aaggttgcgc ttttcccggg ttcgttcgac
     3541 cctattacag tgggacatga atcgcttgtg ctgcgcgcac tatccatttt cgacaggata
     3601 gttgttgcca ttggcaacaa ttccagcaaa aaatcgtact tcgatattga tttacgcaag
     3661 gccatgattg aaaaggtttt tgcaaacgaa cccagggttg aaattatttg ctatgacggt
     3721 ttaacggttg atatctgcaa caagctgaat ataaaccaca tccttcgtgg gcttagaact
     3781 gcagctgatt ttgaatttga acgggccata gcccaggtaa acagggccat gtatccccat
     3841 attgatacta ttttcctact taccgctcct gagcatactt ttgtaaattc ctcagttatc
     3901 agggatatac ttcagcacca cggcgatgcc actaagttta ttcctgcctc catcaacata
     3961 tacgactata tgggctaaaa ccctgcaaaa acccaagggt ggtttacctg cttaggcagg
     4021 atatttgttt actccaccgg ttaaagcaat attacctaac attctagcaa ggctgaaaat
     4081 gagaaatttg gtgctttccc tgttggtgtt tattactctt ggagttacgg gagtattggc
     4141 tcaacaagcc ttagtttacg gcactgcggc cgattatgcc ggtactgagc tggtttttta
     4201 cacctactcc g
//
LOCUS       DAOOQX010000085         4203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_25064_length_4203_cov_3.600289, whole genome shotgun sequence.
ACCESSION   DAOOQX010000085 DAOOQX010000000
VERSION     DAOOQX010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25064_length_4203_cov_3.600289"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="PLX53_02180"
     CDS             <1..1174
                     /locus_tag="PLX53_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOY71918.1"
                     /translation="PKPLISLNDSIHGWERFGEYNDNTFLPPFIEGAWMNKHNGHYYL
                     QYAAPGTEFSGYADGVYIGSNPLGPFRYQEFNPFSYKPGGFARGAGHGATFCDYNQQW
                     WHISTIAIGVKNNFERRIGMWPAYFDADGTLYSQTAFGDYPHYLNGKFTEWMLLNYQK
                     PVTVSSTLYGYYPNFAVDEDIRTYWSASTGNAGEWICTDLLDTCRIYAFQVNYADQDV
                     QLVGKQMGIYHQYRVWGSLDGKSWSLLIDKSENKTDVPHDYVELSTPVEFRFLKLENL
                     HVPAGKFAISGFRVFGKANLPLPESVEFFSVLRGNSEPRNAWLKWKLQDNATGYLINF
                     GVRPDKLYGSIMVYGHNEYYFSGMDRDKTYYFTITPFNESGCGNRSAVVKSEIPLR"
     gene            1270..2025
                     /locus_tag="PLX53_02185"
     CDS             1270..2025
                     /locus_tag="PLX53_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOY71919.1"
                     /translation="MNSWFKSLSASIAKHRVGILTTIIIHLLVIASFLVFKIEDRTEE
                     YASTIEMEFEEPKAQEELLVEEKPEQLLPTDVTVPSREMEAIKNFAVDATQKELNAGL
                     SDEKNTDADNLYSEANRIREQMQENKERYEQAMKEADIAIPNTPEKNIPEEKKNMYKG
                     PTVISYNLAGRKAYRLPVPSYKCEMGGQVVVNIEVLSDGSVSNATIDWANSVNDECIN
                     NSAIMAALASRFSSTQQSTGKEKGSITYLFVAQ"
     gene            complement(2040..3260)
                     /locus_tag="PLX53_02190"
     CDS             complement(2040..3260)
                     /locus_tag="PLX53_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOY71920.1"
                     /translation="MKRTASNLPEFPHAKGLLVQEERLEFVKKNKQLVIGIPKEADST
                     ESRIPLTPEAVEMLVEGGHEIIIEKDAGKQANYNDLDYSEKGGRIVTSSQEVYRSDIV
                     LKVAPLIPAEFELVRDNQIIMSSLHLNSSTGDYLRKLIQKKVTAIAFESICDSDGSFP
                     FIRAMSSIAGSTSVLVAAEYLSNVNKGKGVMLGGITGISPTEVVILGAGTAAEFAARA
                     ALGLGATVKVFDNSIKRLMELQNLLGQRLYTSIFHPQVLARVLKTADVVIGTLTPEES
                     NLRFVVSEEMVMNMKHGSVIVDLAIDKGGCFETSEIRDHNNPSFEKYGVVHYCVPNIT
                     SRVARTASIAMSNVFAPLLLSIGEAGGFQHQLKEDSGLRHGVYVFNGILTNDYLGRLY
                     NLPSRDINLLMAAF"
     gene            complement(3566..3994)
                     /gene="tsaE"
                     /locus_tag="PLX53_02195"
     CDS             complement(3566..3994)
                     /gene="tsaE"
                     /locus_tag="PLX53_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004379084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOY71921.1"
                     /translation="MKSLLIPTIEALDSVASEVAEIVKQYHVVCFYGEMGAGKTTLIK
                     AICHALGVKDIVASPTFALINEYQTRSGQPVYHFDFYRINRIEEVYDFGYEEYFFSTT
                     GICLIEWPELVEDILPSDQTVKLFITVNPDFSRRLDIMVD"
BASE COUNT         1185 a          968 c          952 g         1098 t
ORIGIN      
        1 tccaaagccc ctgattagcc ttaacgacag tattcatgga tgggaacgct ttggcgagta
       61 caacgacaat acctttttac ccccattcat agaaggggca tggatgaaca agcataacgg
      121 acattactac cttcagtatg cggcgcctgg aaccgaattt agcgggtatg ctgatggcgt
      181 ttacattggc agcaatcctc ttggaccatt tcgctatcag gagttcaacc cctttagcta
      241 caaaccaggt ggttttgccc gtggggccgg acatggtgca actttttgcg attacaacca
      301 gcaatggtgg catatctcaa caattgccat tggagtaaaa aataattttg aacgtagaat
      361 aggaatgtgg ccggcatact ttgatgccga tggtacacta tactctcaaa ctgctttcgg
      421 cgactatccc cactacctta atggaaaatt taccgaatgg atgctgctca actatcaaaa
      481 acccgttact gtttcaagca ccctctacgg ttactatccc aattttgcgg ttgatgagga
      541 tattcgcaca tactggagcg cttctacagg caacgctggt gagtggattt gtaccgattt
      601 gctcgacacc tgccgcatat acgcctttca ggtaaactac gcagatcagg atgttcagct
      661 tgttggcaag caaatgggaa tttaccacca gtacagagtc tggggcagcc tcgatggtaa
      721 gagctggtcg ctactgattg acaagtcgga gaataaaacc gatgttcctc atgactatgt
      781 tgagctaagt acgccagtag aatttcgttt ccttaagctc gaaaacctcc atgtcccggc
      841 agggaagttt gccataagcg gctttcgtgt ttttggaaaa gccaacctgc cacttcctga
      901 atcagttgag ttcttctccg tacttagggg caactccgaa ccccgcaatg cctggctgaa
      961 atggaaactt caggataacg caacgggata ccttattaat tttggagttc gtcccgacaa
     1021 actatacgga agcataatgg tttatggcca taatgaatac tattttagcg gaatggatag
     1081 ggataaaacg tactatttta ccataacccc attcaatgag agtggttgtg ggaacagatc
     1141 ggctgttgtt aaatcggaaa tccctttacg ttaaacattt tcaaagcagc taggagttaa
     1201 actaaaattt ttgtaaagca aaacagtatt agccacctga tgaattctaa aaaattgttt
     1261 ggtagtagaa tgaatagttg gtttaagagt ctctcggctt caatagctaa gcatagagtc
     1321 ggcatactca ccaccataat tattcacctg ttggtgattg cgtcattctt ggttttcaag
     1381 atagaggata gaacggagga atatgcatca accattgaaa tggagtttga ggaacccaag
     1441 gcgcaggagg aattgctagt tgaagaaaag ccagagcagc tcctcccaac cgatgtaacc
     1501 gttcccagtc gtgaaatgga ggctataaag aattttgcgg tagatgccac ccaaaaggag
     1561 ctaaacgcag gattgtcgga cgaaaaaaac acggatgccg acaacctgta cagcgaagct
     1621 aaccgcataa gggagcaaat gcaggaaaac aaggagcggt atgagcaggc tatgaaagaa
     1681 gctgacatag ccattcccaa tacgcctgaa aaaaatatac cggaggagaa aaaaaatatg
     1741 tacaaggggc caacggtaat ctcgtataac ctggcaggcc ggaaagcata caggctacca
     1801 gttccctcct ataaatgcga aatgggcggg caggtggttg taaacattga ggtgctgtcc
     1861 gatggcagcg tttcaaatgc cacaattgac tgggcgaact cggtgaatga cgagtgtatt
     1921 aacaactcgg ccattatggc cgcgttggct agtcgttttt cctcaaccca acaatcaaca
     1981 ggaaaggaga aagggagcat tacgtaccta tttgtggccc agtagattgg caaaaccgtt
     2041 tagaatgcag ccattagcag gttgatgtcc cgcgacggca agttgtacag ccgaccaagg
     2101 taatcgttgg taaggattcc gttaaaaacg taaaccccat gccgcaaacc cgaatcctcc
     2161 ttcagctggt gctggaaacc tccagcctca ccaatagata gcagcaacgg tgcaaaaaca
     2221 ttactcatgg caattgaggc tgttcgggca acccgcgagg taatgtttgg cacgcagtag
     2281 tgaaccacgc catacttttc gaacgagggg ttgttgtggt ctcgaatctc agatgtttca
     2341 aaacaaccac ccttatcaat ggccaggtca acaataacgg atccatgctt catgttcatc
     2401 accatctcct cgctaacaac gaagcgcaag ttcgactcct ccggggtaag agttcctatc
     2461 accacatcgg ccgtcttcag cacccgggcc agcacttgcg gatggaatat ggaggtgtaa
     2521 agacgttgcc ccagtaaatt ctgaagctcc attagccgct taatggagtt atcgaacacc
     2581 ttgacagtag cgccaagccc caatgccgca cgagcagcaa actcggcagc tgttccggcg
     2641 cccagtatta ctacctcagt agggctaatc ccggtgatac cgccgagcat aacaccctta
     2701 cccttattta cattacttag atactcagct gccacaagca ccgacgtgct tccagcaatg
     2761 gagctcatcg cccgtataaa aggaaaggag ccgtcggaat cgcatatgct ctcaaaggcg
     2821 atagcggtaa cctttttttg aattagctta cggaggtagt caccagtgga gctgtttaga
     2881 tggagcgacg acatgattat ctggttatcc ctaacaagct cgaactcggc gggaataagc
     2941 ggagccacct taagaactat atcgctgcgg taaacctcct gtgaagaggt aacaatgcgc
     3001 ccaccctttt cgctatagtc taagtcgttg tagtttgcct gctttcctgc atccttctca
     3061 atgatgattt cgtgcccgcc ctcaaccaac atctctacgg cttcaggggt taaaggtatc
     3121 ctgctttctg ttgaatcggc ctctttggga atccctatca ccagctgctt gtttttcttt
     3181 acgaattcca gcctttcctc ctgaactaaa agtcccttag catgtggaaa ttcaggtaaa
     3241 ttcgatgcag tacgtttcat ccgtacaaaa tttttggttt ctgattgcaa ttatcttacc
     3301 ccataccttt taaccttcgg ggttgaactt attgaaatcc catattctgg tttcgccgaa
     3361 ctcgtaagta acgctcggtg gttgaaccgt atttgaggaa gctcccttac atgggcattt
     3421 catgatggtt gttctaaagc tgagaggata tcctaaaaga taaacattcg taatatttgc
     3481 tctcaatccc gccagacttg ggctattata tacgaatctg cgtgaagtaa agttatagta
     3541 atatcgattt ttacacaaaa aaaggttaat caaccattat gtcaagtctc cgggaaaagt
     3601 cgggattaac ggtaatgaat aactttacag tttggtcgga aggtaaaata tcctcaacca
     3661 gctcgggcca ttcgatgagg cagattcctg tggtggaaaa aaaatactcc tcatacccaa
     3721 aatcgtaaac ctcctcaata cggttgattc ggtaaaagtc aaaatggtaa acaggctgcc
     3781 cgcttctggt ctgatactcg ttaatcagcg caaatgttgg gcttgcaaca atgtccttaa
     3841 cgccaagcgc atggcagatt gcctttatga gggtagtttt ccctgccccc atttcgccgt
     3901 aaaaacaaac aacatggtat tgcttaacaa tttcagccac ctcggaggcc acgctatcaa
     3961 gcgcctcaat tgtcggaatt aacaagctct tcattctgaa atgggtttcc tttattgttt
     4021 tgctatagcc agcgtggtag ttgtattcat agagcgggat atgaaataac tgtacgaaat
     4081 ttacacgtat agcaagctag ctggcagtgc atagattact ttggtttata gtgatgttgt
     4141 cgggtatgga tgaatcgtcg gtcaattgag ttttttaacc atctacttcc gctgcatttc
     4201 tcg
//
LOCUS       DAOOQX010000086         4187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_25190_length_4187_cov_4.538722, whole genome shotgun sequence.
ACCESSION   DAOOQX010000086 DAOOQX010000000
VERSION     DAOOQX010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25190_length_4187_cov_4.538722"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLX53_02200"
     CDS             complement(<1..378)
                     /locus_tag="PLX53_02200"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOY71922.1"
                     /translation="METIKIMGTDDTPTVILDAENEIFEISGRSLPEDVTAFYDPVLS
                     WLDGYASSPNPKTIFTFKLVYFNTASSKLLLDVLMKLEQMHEAGHDVLVRWYFPEDDE
                     DMQEAGEEYADIVDVPFEQVSYTL"
     gene            complement(390..965)
                     /locus_tag="PLX53_02205"
     CDS             complement(390..965)
                     /locus_tag="PLX53_02205"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOY71923.1"
                     /translation="MDTKNVKGFLEFVYDFYKSMKAHEITLVYEGEITHQITKAFTSL
                     TESNMAKEEEANSVQKKVFHVMVECLQNISKHADSFGSDDFLFAGRGIFMVSKGDAEY
                     HVTTGNIIENSKIEELSQILDNINNLDKEGLKELYKTQMREGRLSEKGGAGLGFIDIA
                     RKTGRKLDYHFLPIDEDTHFFVLTSTISRTE"
     gene            complement(1005..3617)
                     /locus_tag="PLX53_02210"
     CDS             complement(1005..3617)
                     /locus_tag="PLX53_02210"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOY71924.1"
                     /translation="MTSFRLTSVRETKNLTRNKIALAFLVGLLFSFAAWIADFSKSGL
                     TFSLQNIFRQHAANPTLWITDLIPFAFAYITWLQSTRFMRKTLRLEKELKQRDEDIKK
                     NADFAKKIGEGNYNAPFEVASEDDILGKSLLIMRDNLLTNQKKEWERSWIAKGKEEIS
                     YILRLHHNIDELSYDVLVKLIKYINIIQGALYIYDEEKQLLINMTTYAYNRRKYINQE
                     FRIGQGLIGECAYEKDFIYRTEIPDDYITITSGILGDKKPKSLLLIPLITDEKLEGVL
                     EFASLENEIPELTIQFLKEVGEIIARTIFNLRINQKTERLLQEAQQMTQELRENEEEL
                     RQNAEEMRATHDELEKSNQQLEAKIKEVENAQKRLHSLLENASEIISIYNSEKKLTYI
                     SPSVTKILGYSPQEMMSGKDFDRLTRKGESDFNEMFQQLIENPHIAITIQYTFMKKDG
                     EKIFLEVTGRNLLDDPAIGGIIINSRDITERKRAEKEERMRSKMQSLSENSPDIIMRI
                     NTQGQFFYANPMVEQYLGISYRDLVNQTLTALDISPTLSGFIKDAIKNIKVNKEKVEA
                     EITFASQMGETIMHIAAIPEFNENELETILFVGHDITEAKRIQMEIHDKNRKITESIN
                     YAQRIQTSILPNNRIIRQYLPKSFIYYHPRDVVSGDFPWFFTKDDYIYIAAVDCTGHG
                     VPGALLSFIGYFTLNNVVDHDSSYTAGEILDRLHYGVRKTLKQDRPDADARDGMDIAF
                     CKINPKTKELQYSGAHRPLYLMRNGELIEYKGNRKAIGGIPHPKKAEEDFVNHLIQIQ
                     PGDRIFFFSDGIVDQVGGPDKRKYSSQRIRELITSNPQTPIQDMAKVFSQDFDTYKGD
                     LKQVDDILLIGIEF"
     gene            complement(3660..>4187)
                     /locus_tag="PLX53_02215"
     CDS             complement(3660..>4187)
                     /locus_tag="PLX53_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOY71925.1"
                     /translation="NATNQEESVDYDKLLSRLIPTLPTDNLEKFGEALLSNIARNYEI
                     VQAVLYTRDGDTNLFTFKAGYAYFSESEPVTYSEGETLSGQVAKNRQILNLDQVPENY
                     ITILSGLGKGSPKHLLIAPIVSPDNNSIGVFELAAFKPFDNMQVELFDRLGRKLGELL
                     WSSRPETYLKHSAWT"
BASE COUNT          987 a          878 c         1032 g         1290 t
ORIGIN      
        1 cagtgtgtag ctaacctgtt cgaaaggaac atcaacaata tcggcatatt cttcgcctgc
       61 ttcctgcatg tcctcatcat cctccgggaa gtaccatcta accagcacgt catggccagc
      121 ttcgtgcatc tgctcaagtt tcatcagcac gtcaagaaga agcttggagc tggcggtgtt
      181 gaagtaaacc agcttaaagg tgaaaatggt ttttgggttg ggggacgaag catagccatc
      241 gagccaagat aggacagggt cgtagaaggc ggtaacatct tcgggaagtg agcgaccgga
      301 aatttcaaaa atttcgtttt cggcatccag tataacggtt ggggtgtcgt cagtacccat
      361 tattttgatt gtttccatag gtttagtatt tactcagttc tcgaaatggt tgatgttaaa
      421 acgaagaaat gggtgtcctc atctatgggc aggaaatggt agtcgagctt ccggcctgtt
      481 ttacgggcaa tgtcaataaa tcctagccct gctccacctt tttcggagag ccttccttcc
      541 ctcatttggg ttttgtaaag ttccttaagt ccttccttgt ccagattgtt gatgttgtcg
      601 aggatttggg acaactcctc aattttgctg ttctcaataa tgttccctgt ggtaacatgg
      661 tactcggcat cgcccttgct gaccataaaa atgccgcgac ctgcaaaaag gaagtcatcc
      721 gaaccaaagc tgtcggcatg cttgctgata ttttgaaggc actccaccat cacatggaat
      781 acctttttct gcaccgagtt ggcttcttcc tccttggcca tattcgattc tgtgagggag
      841 gtaaaggctt tggttatttg gtgggtaatt tcaccctcgt aaaccagggt gatttcatga
      901 gctttcatgg acttgtagaa gtcgtaaaca aactcaagaa accctttgac atttttagta
      961 tccatagccc gtaaaattat ataatttact atcaaatagc aatgttagaa ttcgattcca
     1021 attaaaagaa tatcatcaac ttgctttaaa tcacccttgt atgtatcaaa atcctgagag
     1081 aaaaccttgg ccatatcctg aatgggggtt tgtgggttgc tggttatcaa ctccctgatt
     1141 ctctgcgagc tgtacttccg cttgtctggt ccaccaacct ggtcaactat tccgtcggaa
     1201 aagaagaata tcctatcgcc cggctgaatt tggatgaggt ggtttacgaa atcctcttca
     1261 gcctttttgg ggtgaggaat cccacctata gcttttcggt tgcccttgta ctcaattagc
     1321 tccccgtttc gcattaggta gagtgggcga tgggctccag aatactgtag ctccttggtt
     1381 ttagggttga ttttgcagaa tgcgatgtcc ataccatctc gggcatcagc atcggggcga
     1441 tcctgtttca gagttttacg aacaccatag tgaagtcggt caagaatttc gccggcggtg
     1501 taggacgagt cgtggtccac cacgttattc agcgtaaagt atccgatgaa ggagagaaga
     1561 gctccgggaa ccccatggcc tgtgcagtcc acggcggcaa tgtatatgta gtcatccttg
     1621 gtaaagaacc atggaaaatc gccacttacc acatcgcggg ggtggtagta aatgaaagac
     1681 ttgggaaggt actgcctgat aatcctgttg tttggcagta tggaggtttg aatgcgttgt
     1741 gcgtagttta tgctttcggt aatcttacga ttcttatcgt ggatctccat ctggatgcgc
     1801 ttggcctcgg tgatgtcgtg gccaacaaag agaatggttt ccagttcgtt ctcgttgaac
     1861 tctggaattg cagcgatgtg cataatggtt tcgcccattt gggaggcaaa agtgatttcg
     1921 gcctccacct tctccttatt tacctttata tttttaatgg catccttgat aaatcctgat
     1981 agggtagggc taatatccag ggcggtaagg gtctggttaa ccaggtccct gtagcttata
     2041 cccaggtact gctccaccat ggggttggca tagaaaaact ggccttgcgt gttgatgcgc
     2101 atgatgatat cgggggagtt ttcggagagc gactgcatct tgctacgcat tcgctcctcc
     2161 ttttcagctc tttttcgctc ggtaatatcc ctggagttga tgatgatgcc accaatagct
     2221 ggatcgtcga gcaggttgcg cccggtaacc tccagaaaaa ttttctcccc atcctttttc
     2281 ataaaggtgt actggatggt gatggcgatg tggggatttt cgataagctg ctgaaacatt
     2341 tcgttgaaat cgctttcccc cttacgggtt agccggtcga agtccttacc gctcatcatt
     2401 tcttgtgggg aatacccgag tatttttgta accgatgggc ttatataggt cagctttttt
     2461 tcgctgttgt agatggaaat gatttcggag gcattctcga gcagggagtg caaccgtttt
     2521 tgggcgtttt caacctcttt tattttagct tcaagctgtt ggttcgattt ttcgagttca
     2581 tcgtgcgttg cacgcatctc ctcggcgttt tgtcgaagtt cctcctcgtt ttcccgaagc
     2641 tcttgggtca tttgctgagc ttcctggagc aaccgttctg ttttttgatt aatacgaagg
     2701 ttgaagattg ttcgggcaat tatttctcca acttccttga ggaattggat ggtgagttcg
     2761 ggtatctcat tttcgaggga tgcaaattcc agaacgcctt caagtttctc atcggttata
     2821 agtggaatta aaagaaggct cttgggcttt ttatcgccca ggatacccga ggtaattgta
     2881 atgtaatcgt caggaatttc ggtgcggtaa ataaaatcct tttcgtaggc gcattcccca
     2941 ataagccctt gtccgatacg gaattcctga ttgatgtact tacgccggtt ataggcgtaa
     3001 gtggtcatgt tgatgaggag ttgtttttcc tcatcataaa tgtagagggc tccctgaatt
     3061 atgttaatgt acttgataag cttaaccagc acatcgtagg atagctcgtc aatgttgtgg
     3121 tgaaggcgga ggatgtagga aatttcttct ttccccttgg caatccagct acgctcccat
     3181 tccttctttt ggttggtaag gaggttgtcg cgcatgataa gcagcgactt gcccaaaata
     3241 tcgtcttcgc tggcaacttc aaatggtgcg ttgtagttgc cctctccgat tttttttgca
     3301 aaatcggcat tctttttaat gtcttcgtcg cgctgtttta actccttttc cagcctaagg
     3361 gtcttacgca tgaagcgggt tgattgaagc catgtaatgt aggcaaatgc gaaaggaatt
     3421 aggtcggtaa tccacagcgt tgggttggca gcatgttgac ggaatatgtt ttgaaggcta
     3481 aaagtcagcc ccgatttgga gaaatcggct atccatgcag caaaagaaaa gagtaggcct
     3541 acaagaaagg ctaacgctat cttgtttcgg gttagatttt ttgtttctct tactgaggtt
     3601 aatctaaaag atgtcatgct cctgcaaata ttaattattt tttgcggatt atatattttt
     3661 tatgtccaag cgctgtgttt aaggtaggtt tccggacggc tgctccaaag caactctcca
     3721 agtttacggc caagcctatc aaaaagttct acctgcatgt tgtcgaaagg tttaaaggcg
     3781 gccaactcaa aaacgccgat ggaattgttg tcggggctta caatgggagc aatcagcagg
     3841 tgtttgggcg aaccctttcc tagtccggag agtattgtga tgtagttttc gggcacctga
     3901 tcgaggttaa ggatttggcg gttttttgcc acctgtccgg aaagggtttc gccttccgaa
     3961 taggttacgg gctcggattc ggaaaagtag gcatagccag ctttaaaggt gaaaaggttg
     4021 gtatcgccgt cacgggtata gagtacggct tgaactattt cgtagtttcg ggcgatgttg
     4081 gatagaagtg cttccccaaa tttttccagg ttatcagtag gcagggtagg aataagacgt
     4141 gacagaagtt tatcataatc aacagattcc tcttggtttg tagcatt
//
LOCUS       DAOOQX010000087         4179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_25263_length_4179_cov_3.763822, whole genome shotgun sequence.
ACCESSION   DAOOQX010000087 DAOOQX010000000
VERSION     DAOOQX010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25263_length_4179_cov_3.763822"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /gene="queA"
                     /locus_tag="PLX53_02220"
     CDS             complement(<1..972)
                     /gene="queA"
                     /locus_tag="PLX53_02220"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOY71926.1"
                     /translation="MKLSQYKYNLPQDLIAKFPTENRDESRLMVLNRKNHKIEHRIFK
                     DVIDYIDENDVVVFNNTKVFPARLYGNKEKTGAEIEVFLLRELNREQRLWDVLVDPAR
                     KIRIGNKLYFGDDDILVAEVIDNTTSRGRTLRFLFDGPYEKFKETLYKLGETPLPKFI
                     RREVVPEDRERYQTVYAKHEGAVAAPTAGLHFSKQLLKRMEIKGINFAEITLHVGLGN
                     FRSVDVEDLTKHKMDSEQINIPEEAAEIINRAKQNHHRVCAVGTTVLRTLESSVTTSG
                     QLKPFSGWTNKFIFPPYEFSIPDMLITNFHLPQSTLMMMVSAFSGYDF"
     gene            complement(1103..1816)
                     /gene="truB"
                     /locus_tag="PLX53_02225"
     CDS             complement(1103..1816)
                     /gene="truB"
                     /locus_tag="PLX53_02225"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOY71927.1"
                     /translation="MDVNNFNATKEGTAILVDKPLGWTSFNVVGKIRTLIHRATGDRT
                     IKVGHAGTLDPLATGLLVICTGRATKQIDSLMNGKKEYLATLKLGETTPSFDLETPPD
                     GQFPTEHITPTLIAETLKNFMGIQHQTPPLFSAKYINGKRAYKYARKGIEVALEPVPI
                     EIFEMEMLSFEPPLLTLRICCSKGTYIRALARDIGVALGSGAHLVALRRTVSGDFRIS
                     EALSIQELEKIFSSPMQQT"
     gene            complement(2144..2413)
                     /locus_tag="PLX53_02230"
     CDS             complement(2144..2413)
                     /locus_tag="PLX53_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOY71928.1"
                     /translation="MAKNANKPADSSQVEGKFALGKENYKLLLIGFGIIILGFILMAG
                     GGSKDPNVFDPRIFSFRRITLAPIVVLGGFVFEIYAIMKKPKHKS"
     gene            complement(2450..3328)
                     /locus_tag="PLX53_02235"
     CDS             complement(2450..3328)
                     /locus_tag="PLX53_02235"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOY71929.1"
                     /translation="MKNQTKKSAKGRLRNSYISSIISISLVLFTLGILGLLVISSQKL
                     SDYMKENIGFTIYLNDSVSNADANYLKKLLDASTYVRSSQYYSKEDAASLMEKELGED
                     FISYLGYNPLSAFIDVRLKADYANNDSIPKIESWLKQFQQVKEVDYQKSLVNLVNENV
                     SRLGLVVLAFGALMLFIALVLINNTIRLSVYSRRFLINTMKQVGATWGFIRKPFLLRS
                     ILHGLYASIIAIGLLSGLIYGIRNEVADILQIVEPYYIMGIFAAVTLVGIAINLTATL
                     LAVNKFLRLNADDLYY"
     gene            complement(3346..>4179)
                     /locus_tag="PLX53_02240"
     CDS             complement(3346..>4179)
                     /locus_tag="PLX53_02240"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOY71930.1"
                     /translation="ITIDMWVVNKRYMNNDGFVNKRMVEAPFTPTDADKQILADKSPD
                     FRVFNLTVSPFNDASTSFYHKSIGGYHGAKLRRYQDLIDYHLSKGNMAVLNMLNTKYF
                     IQPGQNGPIALPNPDALGNVWFVDSIKLVNNPDEEIQALYNFNPKTTAIVDKKFESLL
                     ADFKPDNSGAGNIELVEYAPNMLKYHSISNQPKLAVFSEIYYPKGWKASIDGKPAPHF
                     RANYVLRAMIVPAGEHRIEFTFDPPMWRVGKSIDLTSSLLLILIFVGWIGWSAFSKKS
                     I"
BASE COUNT         1071 a          921 c          995 g         1192 t
ORIGIN      
        1 gaagtcatag cccgagaatg cagagaccat catcatgagg gttgactgcg gcaggtggaa
       61 gttggtgatg agcatgtcgg ggatgctgaa ctcgtaggga gggaaaatga acttgttggt
      121 ccatccgcta aatggtttca gctgtccgct ggtggtaacg gaactttcaa gcgtgcgtag
      181 cacagtagtt ccaaccgcgc aaaccctgtg gtggttttgc tttgccctgt ttataatctc
      241 ggcggcttcc tcgggaatgt tgatctgttc ggagtccatt ttgtgcttgg tgaggtcttc
      301 cacatcaacc gagcggaagt tgcccagccc tacatgaagg gtgatttcgg cgaaattaat
      361 tccctttatc tccatacgct tgagcagctg cttgctgaag tgcaggcctg cagtaggagc
      421 agctactgct ccctcatgct tggcgtaaac agtttgatag cgttccctgt cttcggggac
      481 aacctccctt cttataaact tgggtaaagg tgtttcgcct agcttataga gagtttcctt
      541 gaatttttcg taggggccgt cgaagaggaa ccgcagcgtt ctccccctgc tggtggtgtt
      601 gtcaataacc tcagctacca aaatatcatc atcaccgaag tacagcttat ttccaatacg
      661 gatttttcgg gcaggatcaa ccagtacatc ccatagccgc tgctcgcggt tcaactcccg
      721 caggaggaat acctcaatct ccgctccagt tttttcctta ttgccataaa gccgtgccgg
      781 gaaaaccttg gtattgttga acaccaccac gtcgttctcg tcaatgtaat caatcacatc
      841 cttaaaaatg cggtgctcaa ttttatgatt tttccggtta agcaccatta aacgtgactc
      901 gtcgcgattt tcggtaggaa acttggcgat caaatcctgt ggcaggttgt acttgtactg
      961 tgatagcttc atattgttgg acggttaatt cttaggacgt ttttccccga atctcatgcc
     1021 ttgagcagct gctaaagctt aaacttttat tgtaggggaa aataattatg caacaaacag
     1081 tcccacgtta tacgtgccat ttttaagttt gttgcatggg gcttgaaaat attttttcca
     1141 gctcctgaat ggacagggct tcggatattc taaaatcacc gcttacagtt cgccgtaggg
     1201 ccacaagatg cgccccggag ccaagcgcaa caccaatgtc acgggccagt gctctaatat
     1261 atgttccctt gctacaacat attcttaggg ttagcaatgg gggctcaaag cttaacatct
     1321 ccatttcaaa tatttcgatg ggtacgggtt ccagggctac ttcaattccc ttacgggcgt
     1381 acttatacgc tctcttccca tttatgtact ttgctgaaaa cagaggagga gtctggtgct
     1441 gaattcccat aaagttcttt agggtttctg caatcagggt tggagtaata tgttctgtcg
     1501 gaaattgccc atcggggggt gtttccaagt caaaagaagg ggtggtttct cccaatttta
     1561 gcgttgccaa gtactccttt ttcccgttca tcaggctgtc aatttgcttg gttgccctac
     1621 ctgtgcaaat aaccagtagg ccggtggcca agggatcaag ggttccagca tggcctacct
     1681 tgatggttct atcaccggta gcccgatgga tgagcgtcct aattttacct accacgttaa
     1741 atgacgtcca tccaagaggc ttatcaacca gaatggctgt tccctccttg gttgcgttaa
     1801 agttatttac gtccataaat tgaaaatgct ggtggccaat aattgtaacg atttatgctg
     1861 tatcatttga aggagagcaa agaagagtct attctaattg gggaaagcat ttaatatttt
     1921 gcatcatgcg atgttggcgg tgtaaatggc cacaaaaaag tagttttgca agatgaatta
     1981 cccaagcagc cttaacggac ataagccggc aagaaacttg atgcgtgcct ttaccgtgga
     2041 gttgtccagc gcaatactac gcgtataaag atgttagcat tgccatcttt acaataccca
     2101 atagcgtaaa attggatagg gtggctattc ctgttgtcgg cgactaactc ttgtgttttg
     2161 gttttttcat aatagcatag atttcaaaca caaagccgcc cagcacaaca ataggggcta
     2221 gtgttatacg cctgaagcta aagattcgcg ggtcaaaaac attggggtcc tttgaaccgc
     2281 caccggccat gagaataaat cccagaatga tgatgccgaa tcctatcagc aacaacttgt
     2341 aattttcctt tccaagcgcg aacttcccct caacctgaga gctatcggcc ggcttgtttg
     2401 catttttggc catatcagcg tagtattaaa tagtttgaaa tgttagctgt tagtagtata
     2461 ggtcatcggc gtttagcctc aggaacttgt ttactgctaa gagtgtagct gttaagttga
     2521 tagctattcc caccaatgta accgctgcaa atatacccat aatatagtag ggttcaacaa
     2581 tctgaagaat atctgcaact tcgtttctta ttccgtaaat aagtccggaa agcaggccga
     2641 ttgcaataat agaggcatag aggccatgca gaatgcttcg gagaaggaat ggcttacgga
     2701 taaaacccca ggtggccccc acctgcttca tagtatttat gaggaaccta cgggagtaaa
     2761 ccgaaagccg gatggtgttg ttaataagca ccagtgcgat gaaaagcatc agggcgccaa
     2821 aagccagcac taccaaaccc aaccggctta cattttcgtt gaccaggttg accagcgatt
     2881 tttggtagtc aacctccttg acttgctgga actgcttcag ccaactttca atttttggaa
     2941 tgctgtcgtt gttggcatag tcagccttca gcctgacgtc gataaaggca gaaagcgggt
     3001 tgtatccaag ataggaaatg aagtcctctc ccagctcctt ttccataagg ctggctgcat
     3061 cttccttgct gtagtactgc gaagatctga cgtaggttga ggcatcaaga agttttttca
     3121 agtagtttgc atcggcgtta ctaacgctgt cgttaaggta aatggtaaag ccaatattct
     3181 ccttcatgta gtcagagagt ttttgggagc taataacgag caagcccagt atgcccaagg
     3241 tgaaaagaac cagagaaatg ctaatgatag acgagatgta ggaatttcgc aaccgcccct
     3301 tagccgattt tttggtttgg ttcttcatgg gtaggatggc ttcaattaga tagatttctt
     3361 actaaatgcg ctccacccta tccagcctac gaatattaaa atcaggagca gggaggaggt
     3421 caggtcaata ctttttccaa ccctccacat tggggggtcg aaggtgaact cgatacggtg
     3481 ttccccagcc ggtactatca ttgcccggag aacatagttt gccctgaagt gcggcgcagg
     3541 ttttccatca atgctggcct tccacccttt aggatagtag atttcagaga aaactgccaa
     3601 ttttggctgg ttggatattg agtggtattt cagcatattt ggtgcgtatt caaccagctc
     3661 aatgtttcct gcaccggagt tgtcgggttt aaaatcggct agcagcgatt caaacttttt
     3721 atcaacaata gcggtagttt tagggttaaa attgtagagt gcctgaattt cctcgtcggg
     3781 gttgttaaca agtttgatag aatcgacaaa ccaaacattt cctagtgcat cggggttggg
     3841 tagggctata ggaccattct gtccaggctg aatgaagtac ttggtattca gcatgttcaa
     3901 aacagccatg ttaccctttg aaaggtggta gtcaataagg tcctggtagc ggcggagctt
     3961 agctccatgg tagccgccta tagacttgtg gtagaaggat gtagaggcat cgttgaaagg
     4021 gcttaccgta aggttgaaca ccctgaagtc cgggcttttg tcggctaaaa tctgcttatc
     4081 ggcatccgtg ggggtaaaag gggcttctac cattcgttta ttaacaaagc catcgttgtt
     4141 catgtagcgc ttgttaacca cccacatatc tattgtaat
//
LOCUS       DAOOQX010000088         4179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_25267_length_4179_cov_2.883608, whole genome shotgun sequence.
ACCESSION   DAOOQX010000088 DAOOQX010000000
VERSION     DAOOQX010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25267_length_4179_cov_2.883608"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..756)
                     /locus_tag="PLX53_02245"
     CDS             complement(25..756)
                     /locus_tag="PLX53_02245"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOY71931.1"
                     /translation="MNGQLKSLLRKALNRLHLDLTKNLRYDRLSKRIISLHLKPDSTC
                     IDVGAHRGEILDLFLQFAPKGHHLAFEPIPSLYNALEKKYSGRCRVYPFVLGDRIGKT
                     PFQLVRNAPAYSGILKRSYSIRHPEVEEIEVEMATLDSVVNEEENIDFMKIDVEGAEL
                     QVLLGARSTLCRCRPNIIFECGLGASDYYGTNPDDLYDFLTGIVGLKISTLKGWLTDS
                     GNPLTRDEFRRHFERNSEYYFFAHP"
     gene            1144..2526
                     /gene="hisS"
                     /locus_tag="PLX53_02250"
     CDS             1144..2526
                     /gene="hisS"
                     /locus_tag="PLX53_02250"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOY71932.1"
                     /translation="MAMASSKPSIPKGTRDFNPEQMVRRNYIFDTIRSVFSIYGYQPI
                     ETPAMENLSTLLGKYGEEGDKLLFKILNSGDFLERVDPSTARPGNSNAVSLAICEKGL
                     RYDLTVPFARFVVQYQNDIVFPFKRYQIQPVWRADRPQKGRYREFYQCDVDVIGSTSL
                     LNEQELVLIINEVFQRLGVRVVVKLNNRKILSGIAEVIGEAEKLTDITVAIDKIDKIG
                     IDGVNSELRERGVSEVAIEKLQPVILLKGNNTEKLGLLKNTLAPSEVGMRGIGEMEEL
                     LELIRQSGELLSEVELDLSLARGLNYYTGTIFEVKAADVEIGSICGGGRYDDLAGIFG
                     MKNLSGVGVSFGADRIFDVMLQLNLFPDSLLSSTQVMFTNFGKSEAAYCLKALRQLRE
                     QGVSAEIYPDPAKMKKQLDYANRRRIKLVVIAGESEMQQNQLSVKNMETGEQATISAE
                     DVVSYIKENI"
     gene            2531..4033
                     /gene="hutH"
                     /locus_tag="PLX53_02255"
     CDS             2531..4033
                     /gene="hutH"
                     /locus_tag="PLX53_02255"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOY71933.1"
                     /translation="MTRIHRISPQPLTWKVLEDITTGDFRLSLSDESKVLIEKCRRYL
                     DVKMEESSVPIYGITTGFGSLCNKTISRNELSQLQKNLVMSHACSTGAEVSSEIVRLM
                     LFLKAHALSLGNSGVQVATVERIIDFYNNDVLPVVYDRGSLGASGDLAPLANLFLPLL
                     GMGDVRYKGKVVEALDVLKEFGWQPIELKSKEGLALLNGTQFMSAHGVSAMLKAFRLI
                     NLSDLIGAISLEAFDGRVEPFHENIQQARPHRGQVETARHIRAILEGSELINRPKKHV
                     QDPYSFRCIPQVHGASRDAIAYVASVILTEVNSVTDNPTIWPDEDLVISGGNFHGQPL
                     ALAYDFLAIALAELGSISERRTAQLILGLRGLPEFLVANPGLNSGLMIPQYAAAAMVS
                     QNKQLCTPASVDSIVSSNGQEDHVSMGANAATKLLRVVDNVERILAIELLNAAQAMEF
                     RRPAKSSPAIESILASYRRQVKFFGEDRIMFKEIDKTLEFIRNIKPGEWL"
BASE COUNT         1093 a          951 c         1074 g         1061 t
ORIGIN      
        1 taaaaattaa tatttaaagc tattctaagg gtgagcaaaa aagtagtact ctgaattacg
       61 ctcaaaatgg cggcggaact catccctggt tagcgggttt ccgctatcgg taagccaacc
      121 cttaagggtt gaaattttca gacctacgat gccggttagg aaatcgtaaa gatcatcggg
      181 gttagtcccg tagtagtcgc ttgcacccag cccgcattca aaaataatat tcggcctgca
      241 acggcaaaga gtgctacggg caccaagtag cacttggagt tcagcaccct cgacatcgat
      301 tttcataaaa tctatatttt cctcctcatt gacaacggag tccagcgtgg ccatttcaac
      361 ctcaatctcc tcaacctcgg gatgtcgtat gctgtagctt cgttttaaaa ttccgctgta
      421 ggcaggcgca tttctaacca gctggaaggg ggttttccct atcctgtctc ccagcacaaa
      481 cggatatacc cggcaccttc ccgagtattt tttttcgagg gcattgtaga gcgaaggaat
      541 aggctcaaag gccaggtgat ggccttttgg ggcaaattgt aggaacaggt caagaatttc
      601 tcccctatgg gcacccacgt caatgcaggt ggagtcgggc tttaggtgta aggagataat
      661 ccttttacta agcctgtcgt acctcaaatt ttttgtgaga tcgaggtgaa gccggttcag
      721 ggctttccta agcaggcttt taagctggcc gttcatatcc aaactgcatg aggttgattt
      781 aagcaggttt tcaaaattag gaatagttgt ggagcaaggg caagaggtgt aaaagtagga
      841 agaatttgcc gataatttat atgattacct atcaacctta aggttatggt agcagcacag
      901 ccgtggccaa ccagctaaat acggcgtaac atgctatatg ctaaaattgt cggacaaccc
      961 cttcaacggg ttgcaaaaaa atagtaggtt tgcagtcgct tactaaatat ccaatacctg
     1021 gatacgccaa ttgccgaaaa atttcagctt gggtattaac aggtaagcgc tttaagtgac
     1081 agcgggtagc taacgttgga gcattaaccc ggttactgat cattaaaact tgaaaaacac
     1141 tagatggcaa tggcaagcag caagccttca attccaaagg ggaccagaga ttttaatccg
     1201 gaacagatgg tccggcggaa ctacattttc gacaccatcc gttcggtgtt cagtatttac
     1261 ggctaccaac ccatcgaaac gcctgccatg gagaacctct ccaccctgct ggggaagtat
     1321 ggcgaggagg gcgataagct actttttaag attcttaact ctggcgattt tcttgaaagg
     1381 gttgacccca gcacagccag acctggcaac tccaatgccg tttcgcttgc catttgcgaa
     1441 aagggactac gctacgacct aacagttccc tttgcccgct ttgtagttca gtatcaaaac
     1501 gatattgtgt ttccctttaa gcgctaccag atacagcctg tttggagggc cgacagaccg
     1561 caaaaaggac gctacaggga gttctatcag tgcgatgtgg atgtaattgg atccacctcg
     1621 cttctcaacg agcaggagct ggttctcatc atcaacgagg tgtttcaacg gttaggtgtt
     1681 cgcgtggttg tgaagcttaa caaccgcaaa atactaagcg gtattgccga ggttattggt
     1741 gaagccgaaa agttaaccga tatcacggtg gccattgata agattgacaa gattggtatt
     1801 gatggggtga acagcgagct cagggaacgg ggtgtatccg aagtcgccat agaaaagttg
     1861 caaccagtta ttttactgaa gggcaacaac actgaaaaac ttgggctgtt gaaaaataca
     1921 ctagcacctt ccgaggtggg gatgagggga attggcgaga tggaagagct gcttgagctg
     1981 attcgacaga gcggtgaact tttatcggaa gttgagctgg acctctcgct ggcacgtggg
     2041 ctaaactact acaccggaac catattcgag gtaaaggctg ccgatgtcga aattggcagt
     2101 atttgtggcg gtggtcgcta cgacgatctt gccggtattt ttggaatgaa aaacctaagc
     2161 ggggttggag tgtcgttcgg tgccgaccgc atattcgacg taatgctgca gctcaacctc
     2221 tttcccgata gcctgctatc ctccacgcag gtaatgttta ccaatttcgg gaaaagcgag
     2281 gctgcatact gccttaaagc tcttcggcag cttagggagc agggtgtaag tgcagaaatt
     2341 tatcccgacc ctgccaagat gaaaaagcag ctcgactatg caaaccggcg taggattaag
     2401 ctggttgtca ttgccggcga gagcgagatg cagcaaaacc agctatcagt gaagaatatg
     2461 gaaacaggag agcaggctac tatttctgct gaagatgttg taagctatat caaagaaaat
     2521 atttaaagcc atgaccagaa ttcataggat ttcaccccaa cccctcacat ggaaggtttt
     2581 agaggatatt accaccgggg atttcaggct atcgctttcg gatgagtcaa aagttctgat
     2641 tgaaaagtgc cgccgttacc tggatgtcaa gatggaggaa agcagcgttc ccatttacgg
     2701 catcaccact gggttcggct cgctttgcaa caaaacaata tcccgaaatg agctgtcgca
     2761 gctccagaaa aatttggtca tgtcccatgc ctgttcaacg ggcgccgagg taagttcgga
     2821 aattgttaga ctaatgcttt tccttaaagc acatgccctg tcgttgggaa actcgggtgt
     2881 tcaggttgca acggtggagc gtatcattga tttctacaat aacgacgtac tgcctgttgt
     2941 ttacgatcgt ggctcgcttg gagcttccgg cgacttggct ccgctggcca atcttttttt
     3001 gcctctgctt ggcatgggtg atgtaaggta taagggaaag gttgtagagg ctctggatgt
     3061 gttaaaggag tttgggtggc agcccattga gctgaagtcg aaggaggggt tggctttgct
     3121 gaatggaacc cagtttatga gcgcccatgg tgtttccgca atgctcaagg catttaggtt
     3181 gataaacctt tccgacctga ttggcgcaat ctccctagaa gctttcgatg gacgggtaga
     3241 gccgttccac gagaatattc aacaggccag gccacatcgg ggacaggttg aaaccgccag
     3301 gcacattagg gcaattctgg agggtagcga gctgattaac cgccccaaaa agcatgttca
     3361 ggatccctac tcattccgct gtatccctca ggttcatggg gcatcaaggg atgccattgc
     3421 gtatgttgcc agcgtaattc tcaccgaggt taactccgtt accgataacc ccaccatttg
     3481 gcccgatgag gatttggtca tttcgggggg caacttccac gggcaaccgc tagccctggc
     3541 ctacgatttt ctggctattg cccttgccga gctgggaagc atttccgaac gccgcacagc
     3601 tcagctcatt ttagggttaa gggggctacc tgaatttctg gtggctaacc ccgggctaaa
     3661 ttccggttta atgatcccac agtacgctgc tgccgccatg gtaagccaga ataagcagct
     3721 ttgtacacct gcgtcggttg attccatcgt ttcatccaac ggccaggagg accacgtgag
     3781 catgggggct aacgctgcaa ccaagctgct gcgcgtggta gataatgttg aacgcatttt
     3841 ggccattgaa ctcctgaacg cagcccaggc aatggagttt cgccgtccag ccaaatcctc
     3901 gccggctatc gaaagcatac tggcaagcta ccgccggcag gttaagtttt ttggtgagga
     3961 tagaattatg ttcaaggaaa ttgataaaac tcttgagttt attagaaata ttaaacccgg
     4021 ggaatggcta taaaagatgt gcggtagagg aaaactgcta gtttatctca accatatcaa
     4081 ccggaatttt gaggactagt cgtaggtcgc ggccccaatc gcgcatgtcg aagtcgtcgc
     4141 tttcgtagtg gcagtttatt cttacttcaa cacccctgc
//
LOCUS       DAOOQX010000089         4177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_25276_length_4177_cov_3.827268, whole genome shotgun sequence.
ACCESSION   DAOOQX010000089 DAOOQX010000000
VERSION     DAOOQX010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25276_length_4177_cov_3.827268"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..258)
                     /locus_tag="PLX53_02260"
     tRNA            complement(185..258)
                     /locus_tag="PLX53_02260"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(222..224),aa:Ala,seq:ggc)
     gene            577..1803
                     /gene="hflX"
                     /locus_tag="PLX53_02265"
     CDS             577..1803
                     /gene="hflX"
                     /locus_tag="PLX53_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOY71934.1"
                     /translation="MGKIFSTEKKREKAILVGVIKQGQEETEIKENLVELDFLAETAG
                     ALSIKQFIQRIESPNPSTYIGKGKVIEVLAFISDNEVDMVIFDDELSPTQLRNLEAEL
                     GIKVLDRTNLILDVFARRAKTAYAKTQVELAQYQYLLPRLAGRWTHLERQRGGIGLRG
                     PGETEIETDRRVIRNRIARLKEELGRIDKQMAIQRSNRGELVRVALVGYTNVGKSTIM
                     NLISKSDVFAENKLFATLDTTVRKVVIGNLPFLLSDTVGFIRKLPHQLVEAFKSTLDE
                     VREADLLLHVIDISHPGFEEQINVVNQTLVEIGASDKPTILVFNKIDAYSWNEKGEDD
                     LLPPDKFNTSLSSLQNSWMAKANVQTIFISARLKTNLDQFKKLLYSSVRNIHVGRYPF
                     DNFLYPGDITFDGPKS"
     gene            1845..2465
                     /locus_tag="PLX53_02270"
     CDS             1845..2465
                     /locus_tag="PLX53_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOY71935.1"
                     /translation="MAELVARITAWYMGNINYTTITILMAIESSFIPFPSEIVVPPAA
                     FKAAQGELNIYLVILSSTFGAIIGAIFNYFISIWIGRKLIYAFAETKFAHLLMIEPKS
                     IQKAESYFVKHGNSSTFIGRLVPGIRQLISIPAGLARMHFGKFILYTTLGAGLWNIVL
                     ALLGYYIPADLVDRYYKELSWAMIILGAAFVLRLGYKAFGKKRQPT"
     gene            complement(2533..3525)
                     /locus_tag="PLX53_02275"
     CDS             complement(2533..3525)
                     /locus_tag="PLX53_02275"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOY71936.1"
                     /translation="MKVAVVGATGLVGQVMLRVLEQRNFPVTELIPVASEKSVGKTLK
                     FKNEALKVSSLEDAVRQKPSLALFSAGSAVSGEWAPRFAEEGTTVIDNSSCWRMHNEI
                     PLVVPEVNASVLSSSSKIIANPNCSTIQLVVAVNPLHQHFRVKRIVVSTYQSVTGTGV
                     KAVNQLFAERQGIATDMAYPHPIDLNCIPHGGSFLDGGYTTEEQKLIDETRKIIGDPH
                     IMVSPTVVRVPVVGGHSESVNIEFEKDFLLEQVYDILKNSPGVSVQDVPSENTYPMPR
                     MAQGRDDVFVGRIRRDVSKPHCLNLWVVSDNLRKGAATNAVQIAEYLLLQNWLK"
     gene            3605..>4177
                     /locus_tag="PLX53_02280"
     CDS             3605..>4177
                     /locus_tag="PLX53_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71937.1"
                     /translation="MKRLVVSIFSLLIFYEVSGQIFYDFENADISGWIQKPAGRWQAS
                     TINPLSGEFSLKHTFDNTSSSTDTIMVSLPSWNVNNGDVTWRMLVRHGYNPSPSNSWW
                     IYLMTGDEIATGTPASGYAVGVNLSGSDDMLKVWRVDNGIPQALLSSTLNWETTIGTA
                     NAGAIEVKRLSNGTFNLKASAGGTFDNLNDY"
BASE COUNT         1178 a          867 c          919 g         1213 t
ORIGIN      
        1 gtccgccagc cggcggatcc caagctgagg agcgcagtcc gcccaccggc ggatcacggg
       61 ttcgagtccc gtttcccgct caggagttca ggctacaggg tattccaccc gtagtttttt
      121 tgctgtaaag ggcatcaata aaaaaacccg tacttcttgg atgtacgggc ttttcttaat
      181 gaggtggagg taaggggagt cgaacccctg acctcttgca tgccatgcaa gcgctctagc
      241 caactgagct atacccccat ttcttgcgca aatatagttt ttttttaaaa tactatcacc
      301 ttataaaatt tccaacttgc taaatgtttg atgaaatcca ctattttaga gttaccttta
      361 acattaatta gtctttttgc cgatattcaa acattaaaga tgcactccga tacttccgat
      421 tttctgaaaa tttagcttaa tgtttggttc taaaacacaa cggttttttg caataactcc
      481 ctcggttaga tatttcatat tatttttgtg cgactatata gtagaatctg ggtaaaaaaa
      541 aactaaagcc agccgttaac aaggctaact ttaaatatgg ggaaaatttt ttcaactgag
      601 aaaaaaaggg agaaggccat tcttgtaggt gtaattaagc aggggcagga ggagacagag
      661 ataaaggaaa acttggttga gttggatttt ttagctgaaa cagctggtgc gttgtccata
      721 aaacagttta ttcaacgcat cgaatcacca aatcccagca cgtacattgg taagggaaaa
      781 gttattgaag tgctcgcatt catttcggat aacgaagtgg atatggtgat ttttgacgac
      841 gagttatccc caacacagct caggaacctg gaggcagaac ttggtattaa ggttcttgat
      901 agaacgaatc ttatacttga tgtttttgca cgacgggcta aaacagccta tgcgaaaacc
      961 caggttgaac ttgcacagta tcaataccta ttacctagat tggcagggcg ctggacccac
     1021 ctcgagcggc agcgtggcgg tattgggcta agggggccgg gtgaaactga aattgaaacc
     1081 gataggcgtg ttattcgcaa tcgtattgct cgtcttaagg aagagctggg ccggattgat
     1141 aaacagatgg ccattcaacg gagcaacagg ggagagttgg tgcgtgttgc gcttgttgga
     1201 tataccaacg tgggcaaatc aacaatcatg aaccttatca gcaaatccga tgtttttgct
     1261 gaaaataagc tcttcgccac gcttgatact actgttcgta aggttgttat cggaaatctc
     1321 ccattcttgt tgtccgatac ggtaggtttt atacggaaat taccccatca gctggttgag
     1381 gcgtttaagt caactctcga tgaggtgagg gaggccgatt tgctgctaca cgttatagac
     1441 atctcccatc cgggctttga ggaacaaatt aatgtggtta accaaacatt ggtagaaatt
     1501 ggggcaagcg acaaaccaac cattctggta tttaacaaaa ttgatgcgta tagctggaac
     1561 gagaagggtg aagacgattt actacctcct gataaattta atactagtct ttccagcctt
     1621 cagaacagct ggatggccaa agccaatgtt cagactattt tcatttcagc acggttgaaa
     1681 accaacctcg atcaatttaa aaagcttcta tacagttcgg taaggaacat tcatgttgga
     1741 cgatacccct tcgataactt tttatatcct ggggatatta cctttgacgg gccgaaaagt
     1801 tgaacattgc actagaaatg taagctaatt cacaaaaaaa aactatggca gaacttgttg
     1861 ctagaattac agcttggtat atggggaata taaactatac cacaattacc atacttatgg
     1921 ctattgaaag ttcttttatt ccctttcctt ccgagatagt tgtacctcct gcggccttca
     1981 aggcggccca gggagagctt aatatatacc tggttatttt atcctcaaca tttggggcaa
     2041 ttattggcgc tatttttaac tattttatat ctatctggat aggacgtaag cttatctatg
     2101 cgttcgctga aaccaagttt gcccacttgt tgatgattga acctaaatct attcaaaagg
     2161 ctgaatcata ttttgttaag catggcaaca gctctacgtt tattggacgg ttagttcccg
     2221 ggattcgaca gcttatctcc attcctgcag ggcttgccag aatgcatttt ggaaagttca
     2281 tcctttacac aactctgggt gccggactct ggaacattgt acttgccctt cttggttact
     2341 atatccccgc cgatttggtt gacaggtact ataaggagct tagctgggcc atgataatac
     2401 tgggggctgc atttgtgttg cgcttagggt acaaggcatt cggaaaaaaa aggcaaccaa
     2461 cctaattttt tgtattaacc tattggtaca gaacaaatat tgatacggtt tgcggagtac
     2521 ctattttgag aactatttta accagttttg tagcaatagg tattccgcaa tttgaacagc
     2581 atttgttgct gcccccttac gtaggttatc tgaaaccacc cataggttca aacaatgggg
     2641 ttttgataca tccctgcgaa ttctgccaac gaaaacatca tccctgccct gggccattcg
     2701 aggcatagga taggtattct ccgatgggac atcctgaacg cttacccccg gtgaattttt
     2761 aagaatgtca tacacctgtt caagcaaaaa atctttttca aactcaatat taaccgattc
     2821 ggaatgtcct cctactacag gaacgcgaac aactgtagga gaaaccatta tatgaggatc
     2881 tccaataatt tttcgtgttt catctatcaa cttctgctcc tcagtggtgt agcctccatc
     2941 aagaaaagat cctccgtgtg gaatgcagtt caggtcgatg ggatgcggat aggccatgtc
     3001 tgtagcaatg ccctgtcgct cagcaaagag ctggttaaca gctttaacac cagttccagt
     3061 aacagattgg taggttgaca caactattcg ttttactcta aaatgttgat gaagcgggtt
     3121 caccgccact accagctgaa tggtggaaca gtttgggttg gcaataattt tagatgaaga
     3181 gctcagcact gaagcgttaa cctcgggaac aacaagtgga atttcattgt gcatacgcca
     3241 gcatgaggag ttgtcaatta ctgttgttcc ttcctcggca aaccttggcg cccattcacc
     3301 cgaaacagct gagccggcag aaaatagggc caaggagggc ttttgcctta cagcatcttc
     3361 caatgaactt acctttaagg cttcattttt aaactttaaa gttttaccta cagatttttc
     3421 ggatgctacg ggaattaact ctgttacagg aaaatttctt tgttccaaaa ctctaagcat
     3481 aacctgacca accagtccgg ttgcgcccac tacggctacc ttcataggtg tgatttttaa
     3541 tactgattga attactgctg tttttttcgt aaattaatcc gcataaaaat agctaaatag
     3601 tacaatgaag cggttggtgg tatcaatttt tagcctgttg attttttatg aggttagtgg
     3661 tcaaattttc tatgactttg agaatgctga tatttcgggt tggatacaaa aaccagctgg
     3721 acgatggcag gcttctacca taaatccgtt atcaggagaa ttttccttaa agcatacatt
     3781 tgacaatacc agttcgtcca ccgataccat catggtcagt cttccaagct ggaatgttaa
     3841 caatggagat gtaacgtgga ggatgcttgt gcggcatggt tataatccct ccccctctaa
     3901 cagctggtgg atttacctga tgacgggaga tgaaattgca acaggcactc ctgcttcagg
     3961 ctatgctgtt ggtgttaacc tatcggggag tgatgacatg ctaaaggtgt ggagggttga
     4021 taacggcatt ccccaggcgc tgttgtcatc taccttaaat tgggaaacaa caataggaac
     4081 agcaaatgct ggggctatcg aggtaaaaag actttccaac ggaacattta atcttaaagc
     4141 ctcggcgggc ggaaccttcg acaatcttaa cgattac
//
LOCUS       DAOOQX010000090         4152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_25481_length_4152_cov_2.192092, whole genome shotgun sequence.
ACCESSION   DAOOQX010000090 DAOOQX010000000
VERSION     DAOOQX010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25481_length_4152_cov_2.192092"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PLX53_02285"
     CDS             complement(<1..785)
                     /locus_tag="PLX53_02285"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HOY71938.1"
                     /translation="MKRIRYILSLAVLAVLASCNPNEMPEFNDNDAFVAFDNASMSVE
                     ENAQTLSIPVTLASIKGISASVTYTLLDGGTAVEGSNFTFADANKTLTFDATNRTQYI
                     VVNIINKPGVFTGDLRFKLQLSDDGTIKPNAENVCTITITDLDHPLAAILGTWTATGT
                     SYFNGSESWDMELTKDPSDVTVVWFYPFVNGGSGYKIYGVVNDAKTEIRVPVGQEIAP
                     PGSYGFIELKGFYGPSGEEEIPDGGYITITIEPNKLSIKDEIGS"
     gene            complement(802..2226)
                     /locus_tag="PLX53_02290"
     CDS             complement(802..2226)
                     /locus_tag="PLX53_02290"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOY71939.1"
                     /translation="MKKSFIILLLTSLVLAGCENFLDEPPRLEQSSDLTLSTYSGLNN
                     SIAGAYGPLASSVWYGAFYVLDAEMRAGNAAIPTNTDFTSGRMLEAKNLNYSPTNTSG
                     LWGYAYYVISAANNVIDNLEGKDVGGVTQQDLNNLKAEALFLRALSHFDLVRLYATSM
                     AKGPDEPGVPYIYHTDQSGTAMPARETVRTVYQNIINDLLEAEAIIDPAYVGAGVDPK
                     ASVSLPAIQALLSRAYLYNQEWQKAADYATKVISNPAYSLWTASQLPNVWNKEIAGPG
                     GEVIFEVYEKKSNSYDAWWEGPSHMTNPIGYADCATHSDLLALYTSTDVRKTLFRTDP
                     DGVSDSFWTTKYKGKGDGDALGTPDANNVIVLRLSEMYLNRAEALVNGATIPGATALS
                     DINAIVTKRGLAPYSSVGADVVFLERRLELAFEGHLWFDYIRTNRPSTRSDKPLAADD
                     YRWLMPIPQREMDVNDNLVQNPEY"
     gene            complement(2245..>4152)
                     /locus_tag="PLX53_02295"
     CDS             complement(2245..>4152)
                     /locus_tag="PLX53_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOY71940.1"
                     /translation="RVNADFKLTKTLETGARMNVAYTESNDVAMQKLYYVNPAFAGMT
                     IQPWTPAYNADGSYNINIPENSNANPRATAEYDDQWEKQYRVQGTYFLTWKPIEKLEI
                     KTNNAAEGTFGEGRRYWSPEAGGGTTGTLQSSTLQYVQLTTSNTVTYNNVFAADHNVR
                     FLVGQEAMRRTYWSYYIYSPDVDPQIPYPTTSTPLVDEGSYDYNARTLMSFFGILDYN
                     YASKYYFQGSLRYDGSSLFGSDKQWGLFWSVGGSWNINKEAFMENVSFLNILKLRASY
                     GVNGNNNIAPYQAYGVYSPSVYNGITGMLPSTPPNSKLSWETNETWNLGLDFGFFKGK
                     LNGSIDAYSRKTKDMLLSKQVPQTSGFSSNFMNIGSLLNEGVEFQLDGEILKTNNLVW
                     NAGFNISYNRTELLNLGDVKEMNYSGDSRLKHTVGKSMYTFYLKDYYGVNPSTGDALW
                     RTSDGTLTKDYNKAAFVYAGSPEPKFIGGFNTSLNWKGFNLSVFLEFKQGNKVLVIER
                     RYINSDGNQMSMNQNVSALNYWKNPGDVGVNPKPVAGNSTSSYVVGSTRWLEDGSYCR
                     IKDITLSYSLPENIVKNLKLGSVMVYVSAVNPYTFHDVDFWDPERGVTGMGAGIYPMT
                     KSFTGGIELSF"
BASE COUNT         1190 a          913 c          844 g         1205 t
ORIGIN      
        1 gagccaattt catccttaat agacagctta ttgggctcaa tagttatggt gatgtagcca
       61 ccatcaggaa tttcttcctc tcccgatggg ccataaaatc ccttaagctc aataaaacca
      121 tagctgcctg gaggtgctat ttcctgtcca acgggtactc ttatctcggt tttggcatcg
      181 tttaccacgc cataaatctt atacccagaa cctccattga caaatgggta gaaccaaaca
      241 accgtaacat cggaaggatc ttttgtaagc tccatatccc aagattcaga cccgttaaag
      301 tagctagtcc cggtagctgt ccaagtgcct aaaatagccg ctagtggatg gtccagatct
      361 gtaatagtta tagtgcatac gttttcggca ttaggcttga tggttccgtc gtcggaaagc
      421 tgaagcttaa acctaaggtc gccggtgaac acaccaggct tattaataat gttaaccaca
      481 atatactgtg tacggttggt ggcatcaaat gttaatgttt tgttggcatc agcaaaggta
      541 aagttactgc cttcaactgc tgttccacca tcaagtaacg tatatgttac ggaggcagat
      601 atgcccttaa tagacgcaag ggtaacaggt atacttaacg tttgggcatt ttcttcaaca
      661 gacatggatg cattgtcaaa agccacaaaa gcgtcattgt cgttaaattc cggcatttca
      721 tttgggttac acgaagcaag aactgccaga actgcgagtg atagaatata acgtatcctt
      781 ttcattttta tcacttattt attagtattc agggttttga actagattgt cattaacgtc
      841 catctctctt tgagggatag gcatcaacca tctgtaatca tcagcagcca gaggtttatc
      901 ggaacgagta gatggcctat ttgttctgat ataatcaaac cataagtggc cctcaaaagc
      961 gagttctaac cgacgttcaa gaaaaacaac atcggcacct actgatgaat atggcgctaa
     1021 tcctctttta gtaacaattg catttatgtc gcttagtgca gttgcaccag ggatagttgc
     1081 accgttaacc aacgcctcgg ctctattaag gtacatttcc gaaaggcgaa gaacaataac
     1141 gttgttagca tcgggcgttc ccaaagcatc accatcgccc ttacctttat acttagtagt
     1201 ccaaaacgaa tctgaaacgc catccgggtc tgttctaaaa agggtttttc taacgtcagt
     1261 tgaggtatat agtgcaagta aatcgctatg cgtagcacaa tcggcatatc caattgggtt
     1321 ggtcatatgc gaaggacctt cccaccatgc atcgtaagaa tttgatttct tttcgtaaac
     1381 ctcaaaaatt acctcaccac cgggaccggc aatttcctta ttccaaacat taggcaactg
     1441 agatgcagtc cacaatgaat atgcaggatt actgataacc ttagtagcat aatcagctgc
     1501 tttctgccac tcctggttgt atagataagc tctgctcagt aatgcctgaa tagcaggaag
     1561 gctaacgcta gctttaggat cgacacccgc cccaacgtat gcagggtcaa taatggcttc
     1621 ggcctcaagc aggtcattga taatattttg gtaaacagtt ctgaccgttt cccttgcggg
     1681 cattgctgtg ccggactgat cggtgtggta tatatatgga actcccggtt catcgggccc
     1741 ctttgccatg gatgtggcgt aaagtcgtac caggtcaaaa tgtgaaagcg ctctcaggaa
     1801 taaagcttct gccttcaggt tgtttaggtc ttgctgcgtt actccaccaa catcttttcc
     1861 ctcaaggttg tcaataacat tatttgcagc ggagataaca tagtaggcat atccccataa
     1921 acctgaagtg ttggtgggac tatagttaag gttttttgct tcaagcattc taccgctagt
     1981 aaaatcggta ttagtaggaa tggcagcatt tcctgcacgc atttctgcgt ccaaaacata
     2041 gaaagccccg taccatacag atgaagctaa cgggccatat gcaccggcaa tggaattatt
     2101 caaacctgaa taggttgaaa gcgttaggtc actactttgc tcaagccttg ggggttcgtc
     2161 taggaaattt tcacaccccg caaggaccaa ggaagtcaac agtaatatga tgaatgattt
     2221 tttcattgct ttaatgttta taagttaaaa tgaaagttca ataccaccgg tgaatgactt
     2281 agtcatagga tatataccgg cacccatacc agttacacca cgttcaggat cccaaaaatc
     2341 aacatcgtgg aaagtgtatg gatttacagc acttacgtaa accatcacgc tacccaactt
     2401 taaattctta acaatatttt cgggaaggga atatgaaaga gttatatcct taatacggca
     2461 atagctgcca tcctccagcc aacgagtgct gccaaccaca taagaactag tagagtttcc
     2521 ggcaacaggt tttggattta cacccacatc gccgggattt ttccagtagt ttagtgcaga
     2581 aacattctgg ttcatggaca tttggttgcc atctgaattt atataacgtc tttcgataac
     2641 caatacctta ttaccctgct tgaattcaag gaatacgctc aggttaaatc ctttccagtt
     2701 cagcgaagtg ttaaatcctc caataaactt aggctcaggg ctaccagcgt aaacaaatgc
     2761 agctttgttg taatcttttg taagtgtgcc atcactggtt ctccataatg catctccggt
     2821 tgaggggtta actccatagt aatctttgag gtagaatgtg tacatgctct taccaacagt
     2881 atgcttcagc cttgagtctc ctgagtagtt catctcttta acatctccta aattcaagag
     2941 ttcggtacgg ttatacgaga tattaaatcc ggcattccat actaagttat ttgtctttaa
     3001 aatttcacca tccagctgaa actcaacacc ttcgtttagc aacgaaccaa tattcataaa
     3061 atttgaagag aacccggatg tttgaggaac ctgtttgcta agcagcatat ccttagtttt
     3121 cctactgtac gcatcaatgc ttccatttaa ctttcctttg aaaaagccaa aatcaagccc
     3181 gaggttccag gtttcgttgg tttcccatga tagcttgcta ttaggtggcg tagagggaag
     3241 cataccggtt atgccgttat aaacactggg tgaatataca ccataggcct ggtaaggtgc
     3301 aatgttatta ttaccattaa caccgtagct ggctctaagc ttaagaatat tcaaaaagga
     3361 aacattctcc atgaaagctt ccttgttaat gttccatgaa ccacctactg accagaataa
     3421 accccactgc ttatcggaac caaaaagcga gctgccatcg tatctgagcg atccctgaaa
     3481 gtagtacttt gaggcatagt tatagtcaag tattccaaag aatgacatca aggttcttgc
     3541 attgtaatca taactacctt catcaactaa tggtgtagag gttgtaggat agggtatttg
     3601 tgggtcaaca tcgggtgagt atatgtaata agaccagtaa gtacgcctca tagcttcctg
     3661 cccaactaga aatctaacat tatgatcggc agcaaaaacg ttgttataag ttacggtatt
     3721 tgaagtagtc agctgaacat attggagtgt agatgattgt aatgttccgg tagttcctcc
     3781 tccggcttcg ggcgaccaat acctacgccc ctcaccaaat gtaccttcag ctgcattgtt
     3841 agtcttgatt tccaactttt cgataggctt ccaggttaaa aaataggtac cctgtacgcg
     3901 atactgtttc tcccactgat catcatactc agcagttgcc cggggatttg cattcgaatt
     3961 ttcaggaata ttaatgttgt aagaaccgtc agcattatat gcaggtgtcc atggctgaat
     4021 tgtcatgcca gcaaaagcgg gattaacata gtataatttc tgcatggcaa cgtcattgga
     4081 ttcagtatag gcaacgttca tacgagcccc tgtttccagt gttttggtaa gtttaaaatc
     4141 ggcatttaca cg
//
LOCUS       DAOOQX010000091         4149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_25504_length_4149_cov_2.801905, whole genome shotgun sequence.
ACCESSION   DAOOQX010000091 DAOOQX010000000
VERSION     DAOOQX010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25504_length_4149_cov_2.801905"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1536)
                     /locus_tag="PLX53_02300"
     CDS             complement(79..1536)
                     /locus_tag="PLX53_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOY71941.1"
                     /translation="MKRLLYIFVLLVLADGVSNAQQLLTVEQCRQMALEHNQRMLIAG
                     EHVQAATSLKSSAKTQFFPSVSANGLYMRTNKKFSLFENDLFLPVIPYSAIDPSTGSF
                     NPNLDPANTFVINPSTLQPVTDANGNPIFQNYTWLPKDQAEVGYKNVYTAGVSLTQPI
                     FTGGKIRETYRIAKYGENLARANEAAERSDIIYKTEEAYWRVVAVGEKVKLVNASIQL
                     LEKLSVDIENFYEEGMVIQNDLLKVKVKLNEAQLNLVKVQNGLALSKMALCQQVGLPL
                     NQEILLADSLVASMDTAVIMQSFTDTALAKRPEIEMLNQTINIAKSGVNLMRSRFMPN
                     IGLSANYMLFNPNPYNGFSENFGGDWNVGVVLNIPIFHWNDRGHTLKAAHSEQRVAEL
                     KMDEAKELISLQVQQAVYQLNESVKKVQMAEENLKQAEENLKVTRNAFDEGKLKTSDV
                     LEAQTLWQNAYSDLIDARMEYRLNITNLKKVTGTL"
     gene            complement(1739..2329)
                     /locus_tag="PLX53_02305"
     CDS             complement(1739..2329)
                     /locus_tag="PLX53_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOY71942.1"
                     /translation="MIGSPHDEMRNSIINAATKAFSRFGFRRTSMDDIARSLRMGKSS
                     IYYYFKGKEEIFRAVVAREASQQRENVKKILESPDDAVSKMRSYVKLRMEFIRHLSSF
                     VEQRLNDDLKSLQLTEKFRKKYDDEEISIITQILEEGSRSGAFKVKDFYMSALAIVTA
                     MKGLELPLVNSTNDENAMNPVIDDLLDILFFGIVKR"
     gene            2726..3874
                     /locus_tag="PLX53_02310"
     CDS             2726..3874
                     /locus_tag="PLX53_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HOY71943.1"
                     /translation="MLFSDIVGQEKVKERLIQLVRENRVPHANLFVGPPGAGKLPLAV
                     AFAQYIMCENRGKSDSCGVCPSCRKVAKLVHPDLHFVFPTKTKEKSDEGADENSEKPD
                     DTISTWRSAIIANPYFGENDWYEMIGMENKQGIISTATASEIIRKLGLKSFENEFKVI
                     IIWLPERMNNQAANKLLKLIEEPPEKTIFLLVSENPTHILKTILSRTQQVMVPPINRE
                     SVALALHDKYNLSPELAHDISRIANGNFYEAIRLKDQESNYFFTSYRKLMRLCYSKNV
                     LGLLDWVDETASLGREDLKHLLVYSLRLTRETLVLNMGVEEVSYLMGEEEAFCHDFSP
                     FITTNNVFSITDELVTAFECITQNGNPTVVLTHMTMRMVKLIPNKKAA"
     gene            3928..>4149
                     /locus_tag="PLX53_02315"
     CDS             3928..>4149
                     /locus_tag="PLX53_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71944.1"
                     /translation="MEKNIGSRGGVITVNEASEHSVCSSKGCTKLKVHDWMADLPMPL
                     QQSDIIEVQFKNTRKGFYRNANQLVLRQGD"
BASE COUNT         1102 a          934 c          913 g         1200 t
ORIGIN      
        1 aatgtcatgt gtcacgcgat tacgcagccg ttctttataa atttcttttg ttattctgtt
       61 aattcctttt gtttgcagct aaagggtacc ggtaaccttt ttaaggttgg taatgttaag
      121 ccggtactcc attcgggcat ctataagatc ggagtaggca ttttgccaaa gggtttgtgc
      181 ctcaagaaca tcggaggttt taagtttgcc ttcatcaaaa gcattacggg taacctttag
      241 gttttcctcc gcctgtttta ggttttcctc ggccatttga acctttttca ccgattcgtt
      301 aagctggtaa actgcctgct gtacctggag agaaatgagc tcttttgcct catccatttt
      361 gagttcggca acgcgttgct cgctatgagc cgctttaagc gtgtgccccc tgtcgttcca
      421 gtgaaaaatt ggtatattca gcactacacc tacgttccag tcgccaccaa aattttcgga
      481 aaatccatta taggggtttg ggttgaaaag catgtagttt gccgaaagcc caatgttggg
      541 catgaagcgc gaacgcatca ggttgacacc cgatttggca atgttgatgg tttggtttag
      601 catttctatt tctggtcgtt tcgccagagc cgtatcggta aaactttgca tgataacggc
      661 tgtatccatg gaggctacca acgagtcggc caaaaggatt tcctggttca ggggaaggcc
      721 tacctgctgg caaagggcca ttttagaaag ggcaaggccg ttctgcacct ttaccaggtt
      781 tagctgagct tcatttagct tcacctttac cttgagcagg tcgttttgaa taaccattcc
      841 ctcctcgtaa aagttttcaa tatcaacgct aagcttctct agaagctgaa tggaggcatt
      901 taccagcttt accttctccc caactgctac cacccgccag taggcctctt cagttttgta
      961 gatgatatcg gagcgctcgg ctgcctcgtt ggcacgtgcc agattttctc cgtacttggc
     1021 aatccggtat gtttccctaa tttttccacc ggtaaatatg ggttgggtca aacttactcc
     1081 ggcggtgtaa acattcttat agcctacttc ggcctgatct tttggaagcc atgtgtagtt
     1141 ttgaaaaatc ggattgccat tggcatcggt taccggctga agcgttgagg ggtttattac
     1201 aaaagtattc gccggatcga gatttgggtt aaagctgccc gtactcgggt cgattgcgct
     1261 gtaggggatt accggaagga acaagtcgtt ctcaaacaag ctgaactttt tgttggtgcg
     1321 catgtacaaa ccatttgcac ttacactcgg aaagaactgg gttttggccg aacttttcag
     1381 cgaggttgct gcctgtacat gctcaccagc aatgagcatc cgctggttat gctccaatgc
     1441 catttggcgg cattgctcaa ccgttagcag ctgctgggcg ttggacactc cgtccgctag
     1501 caccagcaac acaaaaatat acagaagccg tttcatccat tcaggtattt atagtattaa
     1561 taaacagttc gttgtaatat tgcacattta tagctgaagt cgttcacaca aaaagtgatc
     1621 aaccatttcg ggtctgctgt tcattccttt ttgtttgtta ttgctacgaa cgtcacaaca
     1681 gtttacattt tccaaccata catgaatacg tcacgttaaa tgccatcaac acacccggtt
     1741 aacgtttaac gatgccgaag aaaagaatat ccaggaggtc gtcaataacc gggttcatgg
     1801 cattctcatc gtttgtagag ttaacgaggg gtagctccaa acctttcata gccgttacaa
     1861 ttgccagtgc tgacatgtag aaatctttta ccttaaaagc cccgctacgg ctaccctcct
     1921 caagaatttg ggtgatgatg gaaatttcct catcatcgta ttttttccga aatttctcgg
     1981 ttagctgcag gctttttagg tcgtcgttca gcctctgttc cacgaaactc gacaggtgcc
     2041 gtataaactc catacgtagc ttaacgtacg agcgcatttt tgatactgca tcatcggggc
     2101 tttcgagtat tttctttaca ttttctcttt gttggcttgc ttcgcgtgca acaaccgccc
     2161 tgaaaatctc ctcctttccc ttaaagtagt agtagattga acttttgccc attctcaagc
     2221 tacgggcaat atcgtccatg gaggttcttc taaatccaaa gcggctgaac gcctttgtgg
     2281 cggcattgat aatgctattc cgcatttcat catgcgggct gccaatcact tcctgatgca
     2341 tggttaaata ttcgactgtt ttgttaaaac gttccaaaat tactacgtaa ttattttttg
     2401 tcaaggaata ttcgactaaa aagtgttaaa attcgaataa atgttttatg ttgctgataa
     2461 cctttgagtt attcgatagt tttagaaatg tttattggaa agcgaataga aggaagcatg
     2521 aagcgataac tgctaaccgg gtatagctta attgcagcgt ttaacctgta gagtagggaa
     2581 tccgcgggca ggcaaacgag tcttggaatt actttatcct ttaaccctaa acctttatcc
     2641 tttattttcc ctccttctct ctttcagaaa gatttgctaa aaattgtaaa tttgagggaa
     2701 ttttaaatag ttaaattgat actaaatgct ttttagcgat atagttggac aggaaaaggt
     2761 aaaagaacgg ctgattcagc tggtaaggga gaaccgtgtg ccccatgcca acctttttgt
     2821 aggcccaccc ggtgcaggaa aactccccct ggcagttgcc tttgcacagt atatcatgtg
     2881 tgaaaaccgt ggcaagagcg attcgtgcgg agtttgcccc tcatgcagga aagtagccaa
     2941 gttggttcac cccgacctgc atttcgtttt ccccaccaag acaaaggaaa aatcggatga
     3001 gggtgccgat gaaaattccg aaaaacctga cgataccatt tcaacatggc gctcggctat
     3061 tatcgccaat ccctactttg gcgagaacga ctggtacgaa atgatcggaa tggagaataa
     3121 gcagggaatt ataagcaccg ccactgccag tgaaataatc agaaaactag gcttgaaaag
     3181 ctttgaaaac gagtttaagg ttataataat atggctgccc gaacgtatga ataatcaggc
     3241 agctaataag ctactgaaac tgattgaaga accgcccgaa aagactatct ttttgctggt
     3301 gagcgaaaac cccacccata tcctaaaaac catactttca agaacccagc aggtgatggt
     3361 acctcccatt aatcgtgaaa gcgtagccct tgccctgcat gacaagtata acctttctcc
     3421 tgaattggct cacgacatca gccggatagc caatggaaat ttttatgagg ccatcaggtt
     3481 gaaggaccag gaatcaaact actttttcac cagctaccgg aagctaatgc gactttgcta
     3541 ctccaaaaac gtgctcggac tcctcgactg ggttgatgaa acagcttcgc tgggcaggga
     3601 ggacttaaag cacctgcttg tttactccct taggctaacc cgcgaaaccc tcgtgctcaa
     3661 catgggagtt gaggaggttt catacctgat gggggaagag gaagcatttt gtcacgattt
     3721 ctcaccattc attacaacaa ataatgtctt tagcattact gacgagctgg ttactgcctt
     3781 cgagtgtatc acccaaaatg gaaacccaac ggtagtttta acccacatga ccatgaggat
     3841 ggttaagcta atccccaata aaaaagcggc ctagaaggtt cataattttt tgctaaattt
     3901 gacgactttt aattggctaa cctgacgatg gaaaaaaata tagggtcgag gggcggggtt
     3961 ataaccgtga acgaagcttc cgagcattcg gtttgcagct caaagggttg caccaagcta
     4021 aaagttcacg actggatggc cgaccttccc atgccgctcc agcaatcgga tattattgaa
     4081 gttcaattta aaaatacccg aaagggtttt taccgaaatg ccaaccagct cgttcttcgg
     4141 caaggtgat
//
LOCUS       DAOOQX010000092         4139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_25561_length_4139_cov_4.394711, whole genome shotgun sequence.
ACCESSION   DAOOQX010000092 DAOOQX010000000
VERSION     DAOOQX010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25561_length_4139_cov_4.394711"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..617)
                     /locus_tag="PLX53_02320"
     CDS             complement(171..617)
                     /locus_tag="PLX53_02320"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOY71945.1"
                     /translation="MPAIRLTKEFRFEMAHVLEGYDGPCRNIHGHSYKLKVTVIGNPI
                     SQPGHPKLGMVIDFGDLKQVVETLITSRFDHSIAVLENSNIHRKLAGSDLEKVEALPF
                     QPTCENMVEYFARLLAPSLPEGVSLHHLLLHETATSYAEWYADDNP"
     gene            867..1715
                     /gene="nfo"
                     /locus_tag="PLX53_02325"
     CDS             867..1715
                     /gene="nfo"
                     /locus_tag="PLX53_02325"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOY71946.1"
                     /translation="MKFVGAHVSAAGGVENAPVNANAIGATAFALFTKNQRQWVAPPL
                     SPKSIDLFRRRCEEFSYSPAQVLPHDSYLINLGNPDRDALEKSRNAFTDEMARCELLG
                     LDRLNFHPGSHLNKTTPEECLNLIAESINMALDKTHGVTAVIENTSGQGSNLGFTFEQ
                     LRYIIDRVDDKTRIGVCIDTCHAFTAGYDLLTPEAFTKTFSEFDRIIGFGYLRGVHLN
                     DSKKGLGSRVDRHENIGLGLLGAEPFRMLMGDPRFDGIPLILETPDESKWPEEIALLK
                     GFDREI"
     gene            complement(1778..1848)
                     /locus_tag="PLX53_02330"
     tRNA            complement(1778..1848)
                     /locus_tag="PLX53_02330"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1814..1816),aa:Gln,seq:ttg)
     gene            complement(2038..3603)
                     /locus_tag="PLX53_02335"
     CDS             complement(2038..3603)
                     /locus_tag="PLX53_02335"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOY71947.1"
                     /translation="MKKTVRVSLSGFSYNLEEDAYRLLDHYLEEIRVRLGSNADAEET
                     LRDIEERISELLTARMGQRETVPADMVQDIINQIGMPEEVTGNAEYSKDQWETQPPKK
                     RLYRDTENPVIAGVCSGVAAFFGVDPIVIRIIFIALLFARGFGLLLYIILWIVVPRAL
                     TPKQKLEMRGQPVTLSNMGKNISEEMSSVKSSVSQKVPRNFFERLGKFLGQVGFWLLK
                     FIMVLVKVVAIAIGIVLIVSMLFAFIVLVGAFFFSGITLPWLFPGTVVSSLNEFITSM
                     IDISSGLWITIPTFLILAIPIVALIYIGIRVLFRFKARDGKIAIIATGIWVASVLTLA
                     ISIFIQLRSLSFSGADRQVVQLSSQLPRNQQTIYLKAFPQYDEATLPSVYKFFDYSIT
                     TVQGEKVISGQPKLVIEKSDSDSISLILSKNARGFSSTNAAKNAADIIYPYSVKDSTI
                     FVDSRFTLPASVTWKGQTLTLSLLLPEGYSIYLDSSICGILDTDQPYSSHWPDEMVGQ
                     TWTMTRNGLRVKR"
     gene            complement(3838..>4139)
                     /locus_tag="PLX53_02340"
     CDS             complement(3838..>4139)
                     /locus_tag="PLX53_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOY71948.1"
                     /translation="QMRKGILELCILSVLSKGDAYANDLITKLKEAKMIVVEGTLYPL
                     LTRQKNLGLLSYRWEESPQGPPRKYYSLTDKGRTYLNELHRSWDELVETVNTLRN"
BASE COUNT         1088 a          945 c          996 g         1110 t
ORIGIN      
        1 gaggcactgc ctgaaaaact ggacgcaggt gtgcttcgca atactacgcc caacctcgtc
       61 gggcgaggaa tttagttata gcgttaacct aataggacac ttggagcaag cttgctgctt
      121 gccctaagaa cagatgaaac aaacgaaatg cttttttcac cggaagcggt tcaagggtta
      181 tcatcggcat accattcggc gtaagaggta gccgtttcgt gcagcaagag gtggtgaagg
      241 gaaacgcctt cgggtaggct gggtgccaga aggcgggcaa aatactccac catgttctcg
      301 caggtgggct gaaaaggtaa agcctcaact ttctcgaggt cggagccagc gagcttccta
      361 tgaatgttgg aattttccaa aaccgcaatt gaatggtcga aacggctggt aatgagggtt
      421 tcaacaactt gcttcagatc gccaaagtct ataaccatcc ccagcttggg gtgtccgggt
      481 tggctgatgg ggttgccgat tactgttact tttagcttat aggagtgccc atgtatgttc
      541 cggcatggtc catcgtaacc ctcaagaaca tgggccatct caaagcgaaa ctctttggtt
      601 agacgaattg ctggcattgt agaaaagtta gccgttaccg cattacggta atcattttta
      661 caaatatagt gaacgttcgt cgacgaagtt tgttaaattg tcagatattt tcgacttttg
      721 atgatcagga tgttctggcc tttagtccga tgacagcaag cttcccatgg aaatggtaat
      781 caacctgagg tttttagggt gggggagaaa acaggtattt ggtaaaggca tttcttcaga
      841 ttgcttatgt taaaatccat caggatatga aatttgtagg agcacacgtt agcgcagcag
      901 gcggggtgga aaatgcaccg gtaaacgcca acgccattgg agctactgcc tttgcgctgt
      961 ttaccaaaaa ccaaaggcaa tgggttgccc cccccttatc gcccaagagc atagatttgt
     1021 ttcgtaggcg ttgtgaggag tttagctaca gccctgcaca ggttttaccc cacgatagct
     1081 acttgataaa tttaggaaac cccgacaggg atgcgctaga gaaatccaga aatgccttta
     1141 ctgacgagat ggcaaggtgt gagctgctgg gtcttgacag gctcaacttc cacccaggaa
     1201 gccacctaaa taagactacc ccggaggaat gcctcaacct gattgccgag tctatcaaca
     1261 tggcactgga taaaacgcac ggcgttacgg cggttatcga gaatacatcg gggcagggga
     1321 gcaacctggg gttcaccttc gaacagctgc ggtacatcat cgacagggtt gatgacaaaa
     1381 cccgcattgg cgtttgtatt gatacctgcc acgcatttac ggcaggatac gacctgctta
     1441 cccccgaggc atttaccaaa acattttctg aatttgatag gattatcggg tttggctact
     1501 tgaggggagt tcacctaaac gattccaaaa aggggcttgg aagcagggtt gaccgccatg
     1561 agaatattgg tcttggtttg ctaggtgctg aaccattccg aatgctaatg ggcgatcccc
     1621 gtttcgacgg tattccgctt atcctcgaaa ctcccgacga atcgaagtgg cccgaggaaa
     1681 tagccctgct caagggcttt gacagggaaa tttgaggatt gcctccagct cggcttaaaa
     1741 ataaaagcat tccgttttca taggaatgct tttaaagttg acctaccagg attcgaacct
     1801 agactaacag aaccaaaatc tgctgtgcta ccattacacc ataggtcaat acccctagtt
     1861 atagtggggc aaaagtaatg gattttttat cacccgccaa aaaattttgg ctattcattc
     1921 gccatctcat tatgggcaaa ggtagtcttt gcgcatagca taacattgtt gtatctaata
     1981 cacgcagcct tcaccaggag caattaagca acaactcatc gtatgggcaa ttgctaccta
     2041 tctttttaca cgtaatccat tgcgggtcat agtccaggtt tgcccgacca tttcatcggg
     2101 ccagtggctg ctgtagggtt ggtcggtgtc caggatgccg caaatgctag agtcaaggta
     2161 aatgctgtat ccttccggaa gtaaaaggga taaagttagt gtttgaccct tccacgtaac
     2221 cgaagctggt agagtaaacc ggctgtcaac aaatatggtg gaatctttca ccgagtatgg
     2281 gtaaatgata tccgctgcat ttttagctgc atttgtgctg ctgaatccgc gtgcattttt
     2341 tgataaaatt aggctgatac tatcggaatc cgatttttca ataaccagct tcggttgccc
     2401 ggaaattacc ttttcccctt gtactgttgt tatggagtag tcgaagaatt tatataccga
     2461 gggtagcgtt gcttcatcgt attgcgggaa tgctttcagg tagatggttt gttggtttct
     2521 gggcagctgg ctgctcagct gcacaacctg cctgtctgct ccggaaaaac ttaggcttcg
     2581 tagttgaatg aaaatgctga tggcaagggt tagcacggag gcgacccaga tccctgttgc
     2641 aataatcgca attttcccgt cacgtgcctt gaacctgaac aaaacccgaa tgcctatgta
     2701 gattagggcg acaatgggaa tggctagaat aaggaaggtg ggaatagtga tccataggcc
     2761 gctacttatg tcaatcatcg aggtgataaa ctcgtttagc gagcttacaa ctgttccggg
     2821 gaacagccag ggcagggtaa ttccgctaaa gaagaatgct cccaccagta caatgaaagc
     2881 aaagagcatg gaaactatca gcacaatgcc tatggcaata gccacaacct ttacgagaac
     2941 catgataaat ttcaacagcc agaagcctac ctgacccaga aactttccaa gacgttcaaa
     3001 aaagttcctt ggaacctttt ggctaacgga gctcttaacg ctgctcatct cctcagaaat
     3061 atttttgccc atattcgaca gcgtaaccgg ttgtcccctc atttcgagtt tctgcttagg
     3121 tgttagcgct ctgggaacaa caatccagag aatgatgtac aggagcagcc caaaccctct
     3181 ggcaaacagt agcgcaataa aaatgattct gattacaatt ggatcaaccc cgaagaaggc
     3241 agcaacgccc gagcaaacac ctgcaattac agggttttca gtatcacggt aaagcctctt
     3301 tttgggtggc tgagtctccc actgatcttt actgtattca gcatttccgg taacttcctc
     3361 gggcatacca atctggttga taatatcctg caccatgtcg gccggaacag tctcacgctg
     3421 gcccattcgg gcagtaagca gctcggaaat ccgctcctca atatccctta aggtttcttc
     3481 ggcgtcagca tttgaaccca acctcaccct gatttcttca agataatggt cgagcagcct
     3541 gtatgcatct tcttccaggt tatacgaaaa gcccgaaagg ctcactctta ctgttttttt
     3601 catttgcttt aatttacttt acggttggca acaaagtagt gtttttatgg taaaatggtt
     3661 ggttaaacca tccttgggct aaacttctgc ctcacctcac ctaaacgaaa gctgaattac
     3721 ggctactttt tatcgtcggt atgacatgct ggcgaaagta tggacgatta agtgtcgtgt
     3781 tgaagttcag catcataggt taaggttaaa agttacggca ggtatttctt gttattatta
     3841 gtttcgaagt gtattcactg tctccaccag ctcgtcccag ctgcgatgca gctcgtttag
     3901 gtaagttctc cccttgtcgg tgagggagta gtacttgcgc ggtggcccct gtggcgattc
     3961 ctcccagcgg tagctaagta atcccaagtt tttctgccgg gtaagcagtg gatagagggt
     4021 tccctcaacc acaatcattt tggcttcttt taacttggtt ataagatcgt tggcgtaggc
     4081 atcgcctttt gaaagcaccg agagtatgca taactccagt attcctttgc gcatctgcg
//
LOCUS       DAOOQX010000093         4113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_25800_length_4113_cov_2.695170, whole genome shotgun sequence.
ACCESSION   DAOOQX010000093 DAOOQX010000000
VERSION     DAOOQX010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25800_length_4113_cov_2.695170"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1568)
                     /locus_tag="PLX53_02345"
     CDS             complement(84..1568)
                     /locus_tag="PLX53_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOY71949.1"
                     /translation="MNRLSTLLFGILSAMPVMSCIAQQPMNRLTYPEVLQLAKEQSPQ
                     AILAKHRFRAAYWEYRTFVAEYRPTLSMNGTIPEFSRSLIRYQNPDGSYSYIEENANT
                     TSLGLSLSQNIGFTGGQIFVKSNLERTDLLGDSSSTSYLSVPVSIGFSQPLFAMNRLK
                     WNKRIEPIKYEEAKKSFIQSMEDISIEASRLFFNLAMAQQNLETARLNYSNTDTLYKI
                     AQGRYNIGTIAENELLQMELSFLNAGTQVNEAEVDLQLKKIQLKSFLGLNDQYDLEII
                     LPTAIPQFELIYDKVMMLARENNPQILSYERQLIEADRDVAQARSERGFNASLYATYG
                     LTQRALNVSDAYKDPQDQQNVRVGITIPILDWGLGKGKVKMAQSNREVIKTTVQQAMT
                     DFEQDIFLKVMQFNRQDDQVMIAEKADYISQNRYEVTKQRFLIGKIDVLDLNVAQSER
                     DQAKQKYISTLGNYWQFYYQMRRLTLFDLQNNQPLQADFEMLLK"
     gene            complement(1590..2975)
                     /locus_tag="PLX53_02350"
     CDS             complement(1590..2975)
                     /locus_tag="PLX53_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY71950.1"
                     /translation="MSMLRVDKLYKRYLHDINIAIEAIMANKLKSALTALGIIFGVAA
                     VISMLAIGNGAQQEILEQMKMVGVNNIIVNSIFEAPDTQTSDEENGTKQKQKFSPGLT
                     LKDVEAIQQIIPSVKRISPEISLNSFVQYDGIRMPAKIMGVSNDYFSLFNLPLEDGVI
                     FDSYHEEHGVPVCVIGSNIKTKFFSTQNPIGQYIKFGSVWLRVIGVLAKSDIKVTGFE
                     SSGINVYNDNIYVPVKSILLRYQNRALVNTRKISESGTSVFVSGGFVSYGGSTQSQAA
                     SNYHQLDRIILQVNQTEDITSTSEVLSRMMLRRHSEVKDFEITVPELLLKQQQRTKDI
                     FNIVLGAIASISLIVGGIGIMNIMFASVMERIKEIGTRLAIGAKKADIIAQFLSEAVL
                     ISVSGGIIGVILGFVLSFLIRHFAGILTIVSPLSILVAFGVSAAVGVIFGLSPAKRAA
                     EKDPIESLRYE"
     gene            complement(2975..>4113)
                     /locus_tag="PLX53_02355"
     CDS             complement(2975..>4113)
                     /locus_tag="PLX53_02355"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOY71951.1"
                     /translation="EKSLQAFLKVQLDTTLQLRELRNNLINLKFDMEEKQLVLEQSKY
                     EPPATQRQAQINLDKAQRAYEQEVHNYTLKKEQAEASMKEVAINLQRQKRERQDMLDV
                     LDKFEIRAPKPGMLIYYREWNGQKRKVGSSVSPWDLIVATLPDLSVMNSSTYVNEIDI
                     SKIKTGQPVRITVDAFPEKKFTGEVIEVANIGEQLPNTDAKVFEVTIRVNEYDPILRP
                     SMTTGNQIITNVYDSVTYIPLEALHANDSISYVYTRKGKMQVVVPGEMNENFIIIEKG
                     LEANDEVYLSMPEKPEKFSLAGKELISVIKKKKEQKIQDEMRLREVQDSAMKSRSQSQ
                     KMMFQMNGQQMRKNGSAPKINSELKGKGNSGSNQANPKPTAPKS"
BASE COUNT          944 a         1001 c          987 g         1181 t
ORIGIN      
        1 tactcttcgt gcagattatg attttagctg aattggtgca gcttatcagc gctcgtgctg
       61 taactcactg aaaaaggcaa atcttactta agcagcattt caaaatcggc ctgaaggggt
      121 tggttattct gaaggtcgaa gagcgttaac ctgcgcatct ggtagtaaaa ctgccagtag
      181 tttcccaggg tgctgatgta cttttgcttg gcctgatcgc gctcggactg ggctacgttt
      241 aaatcgagca catcaatctt gccgatgagg aatcgttgct tggtaacctc gtacctgttt
      301 tgtgaaatgt agtcggcttt ttcggcaatc ataacctggt cgtcctgacg gttgaactgc
      361 atcaccttta aaaagatatc ctgctcaaaa tcagtcatgg cctgctgtac ggtagtttta
      421 attacctcgc gattcgattg ggccattttc acctttccct tgcccaatcc ccagtcgaga
      481 ataggaatgg taatacccac cctaacattc tgctgatcct gcgggtcctt gtaggcatca
      541 gaaacattga gtgccctctg ggtcaaccca taggtagcgt ataggctagc gttaaatccc
      601 ctttcggaac gggcttgagc cacgtcacgg tccgcttcga tgagctgccg ttcgtaggaa
      661 agaatttgcg gattgttctc cctggccagc atcatcacct tgtcgtaaat aagttcaaat
      721 tggggaattg ctgtgggtag tattatttcc agatcgtact ggtcgtttag ccccaggaac
      781 gacttaagct gaattttctt aagctgaagg tccacctcag cctcgttaac ctgagttccg
      841 gcattaagga agctgagctc catctgtagc agctcgtttt cggcaattgt accgatgttg
      901 tagcgccctt gggcaatttt ataaagggtg tcggtgttgg agtagttaag cctggccgtt
      961 tcgaggtttt gctgggccat ggcaaggtta aaaaacaacc tgctggcctc aatggaaata
     1021 tcctccatgg actggataaa gcttttcttg gcctcctcgt acttaatagg ctcaattcgc
     1081 ttattccact ttaggcggtt catggcaaac aggggctggg agaaaccaat ggaaacaggc
     1141 acgctaaggt aggaagtgga gctgctgtcg cccagcaggt ctgttcgttc caggttgctt
     1201 ttaacgaaaa tctggcctcc ggtaaaccca atattttgcg ataacgatag gccgagtgag
     1261 gtggtgttgg cattctcctc aatgtagcta tacgacccgt caggattttg gtagcgaatt
     1321 agacttctgc tgaattcggg aattgttccg ttcatgctca gcgttggcct gtattctgcc
     1381 acaaatgtac ggtactccca gtatgccgct ctaaacctat gcttggcaag gatggcctgg
     1441 ggcgattgtt ctttggccag ctggagcacc tccgggtagg ttagccggtt catgggctgc
     1501 tgggcaatac agctcattac cggcattgct gatagtatcc cgaatagtag ggttgacaac
     1561 ctgttcatct caacaaatta ttagcgtttt tactcgtagc gtaacgactc aatagggtct
     1621 ttttcggcag cacgcttggc aggcgacaag ccaaaaatga cgcccacggc agccgatacc
     1681 ccaaatgcaa ccaaaattga aagtgggctg actatggtaa gtattccggc aaagtgcctg
     1741 attagaaacg atagcacaaa gcccagtatc actccaataa ttccgcccga aacgctgatg
     1801 agcactgctt cggaaaggaa ttgcgcaatt atgtcagctt tcttggcccc aatggccagg
     1861 cgtgttccta tctcctttat acgctccata accgaggcaa acatgatgtt cataattcca
     1921 atcccgccaa caatgagcga gatgctggca atggccccca gcacaatgtt gaatatgtcc
     1981 ttggtgcgct gttgctgctt gagcagcagc tctggaacgg ttatctcaaa atccttcacc
     2041 tcggaatggc gcctgagcat catccggctg agcacctcgg aggtggaggt aatatcctcg
     2101 gtttggttca cctgcaggat aatccggtcg agctggtggt agttggaggc cgcttgtgat
     2161 tgcgtgctac ctccatagga gacaaaccca ccgctgacga atacgctagt tcccgattca
     2221 ctaatcttgc gggtgtttac cagcgccctg ttctggtagc gaagcagtat gcttttcacc
     2281 ggcacgtaga tattatcgtt gtacacgtta ataccgctgc tttcaaatcc ggtaacctta
     2341 atgtccgatt tagccagcac gcctatcacc cttagccata cactgccaaa cttgatgtac
     2401 tgcccaatgg gattctgggt gctaaaaaac ttggtcttta tatttgaacc tataacacaa
     2461 accggaacac catgctcctc gtggtagctg tcgaagatta ccccatcctc caggggaagg
     2521 ttaaagaggg aaaagtagtc gttggatact cccataattt tagcgggcat acgaattccg
     2581 tcgtactgga caaacgagtt aaggctgatt tcggggctaa tccttttaac cgaggggatt
     2641 atctgctgaa tggcctccac atcctttagt gtcagcccgg gcgaaaattt ctgtttttgc
     2701 ttggtgccat tctcctcatc cgaggtttgg gtgtcgggtg cctcgaatat ggagttaacg
     2761 atgatgttgt taacgccaac catcttcatc tgctccaata tctcctgctg ggcaccattc
     2821 cctatggcca acatgctgat aactgcagcc acaccaaaga ttatgcccag cgctgttagg
     2881 gccgatttta gcttattggc cataattgcc tcaatggcaa tgttgatgtc gtgcaggtag
     2941 cgcttgtaga gcttgtcaac tcgcagcata ctcatcagga tttaggagcg gttggtttag
     3001 ggtttgcttg gttgctacca gagtttccct tccctttaag ctcactgttg attttcggag
     3061 ctgagccatt tttcctcatc tgttgcccat tcatctgaaa catcattttc tgggactgag
     3121 acctgctttt cattgcagaa tcctgcacct cgcgaagcct catttcatcc tgaatttttt
     3181 gctccttttt cttctttata accgaaatga gctccttccc cgcaagcgaa aacttttcgg
     3241 gtttctcggg catcgaaagg taaacctcat cgtttgcctc cagccctttt tcaataatga
     3301 tgaaattctc attcatttcg ccaggaacca ccacctgcat tttcccctta cgggtataga
     3361 cgtagctgat ggagtcgttc gcatggagcg cctcgagagg aatgtaggtt accgagtcgt
     3421 aaacattggt aattatctgg ttaccggttg tcatggaggg tcgaagtatc gggtcatact
     3481 catttaccct gatggttact tcaaaaacct tggcatcagt gtttgggagt tgctcgccaa
     3541 tattggctac ttcaataacc tcaccggtga attttttttc aggaaaggca tcaacggtaa
     3601 tacgaacggg ttgtccggtt ttaattttac tgatgtctat ttcgttaacg taggtgctgg
     3661 agttcattac cgaaaggtcg ggaagggtgg caacaatcaa atcccagggt gaaacgcttg
     3721 accccacctt gcgtttttgc ccattccact cgcggtagta gataagcata cccggcttgg
     3781 gtgcacgtat ttcaaactta tcaagcacat caagcatatc ctgcctttct cgtttttggc
     3841 gttgaaggtt gatggccacc tccttcattg aggcttcggc ctgctccttt ttcagggtat
     3901 agttgtgaac ctcctgctcg tatgcacgct gagccttatc aaggtttatc tgagcctgcc
     3961 gttgggtagc cggtggttca tacttcgact gctccagcac cagctgtttc tcctccatgt
     4021 caaacttcag gttgataagg ttgttcctta gctcgcgcag ctgcagggtg gtgtcgagtt
     4081 gaactttcag gaaagcctga aggcttttct cca
//
LOCUS       DAOOQX010000094         4104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_25865_length_4104_cov_3.440109, whole genome shotgun sequence.
ACCESSION   DAOOQX010000094 DAOOQX010000000
VERSION     DAOOQX010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25865_length_4104_cov_3.440109"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="PLX53_02360"
     CDS             complement(<1..1669)
                     /locus_tag="PLX53_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71952.1"
                     /translation="MDKRNRLVERSDEMRYAKYLILKLAMMKQNAVINPRMPMLKRFL
                     GLLLLTLSGYMVAAQRDIEPRLVFSDTSRFNFTGEWQYLSTDIFLLNGDRFSNLINEL
                     NFPNKGNGKIRRKSKLEEERLEYLFITAKLKNVKFFGENDITYPLYNFQISRDKDNRY
                     QTLVSDNIDHVRIIDNLPLYSAHDYIDAEISVKAITNNDRDQMLSLVATQLQNLSKIK
                     TPTDAVMGIIGEFGSFIEANTRKKEYRFSSTIRLFEQKNFDTRVHSLKLYVLTTSNSK
                     PIEVNTALIQNFMDTVRHGQVGRQTLKELLAYHNYPVIAVVNYKSLYRMEPIGGDEVT
                     FASIETRKLRVENDYRQGLINAETYRQEKDFIAFLSVYANFKSYLDAYNLNYRTGNSD
                     AVSISLFKVMQYYRQLVKTFEEMRFKYRGNNTFANIFRREYESVLGFASLYLDDDHNL
                     KATKELVNSLVKLDTIPMLAAADMEAYIASLRFSGAFKPDLMRQTPEGQLITNHLASL
                     EEQLYRSTYEDEVRKLNDVEASASTRNATEKLLAMIKNSSCGLCRERA"
     gene            complement(1672..2844)
                     /locus_tag="PLX53_02365"
     CDS             complement(1672..2844)
                     /locus_tag="PLX53_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71953.1"
                     /translation="MSFVLKEVDNRKTRKDFLNLPVELYRDYPSWIRPLNNDIESVFN
                     PKTNKQFRHGEAIRWVLYSDNKPVGRIAAFIDYEIARNNEQPTGGVGFFECINSPEAA
                     TQLFDTAKEWLQQRGMEAMDGPINFGDRDRWWGLLVEGDYPPNYCMDYHPPYYRELFE
                     GYGFKPYFHQFTYYRPVNPEGLDPIIREKAERIARNPAYHVTIISKRNLKKFAEDFRT
                     IYNNAWGRYTGVKKMSHIHAMALMKTLKPIIDPELMYFAYYNNEPIGFFIMVPDLNQV
                     VRHLNGQFGLVHKLKFLYLLKVKKVCTKAIGLIFGIVSQHQGKGVEGALVTEFANRAL
                     RPDFQYKELELNWIGDFNPTMRRVASQIGGKIRKIHITYRYMFDRTKEVVPPRRVS"
     gene            complement(2869..3555)
                     /locus_tag="PLX53_02370"
     CDS             complement(2869..3555)
                     /locus_tag="PLX53_02370"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF001186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOY71954.1"
                     /translation="MQPKRFALVVAAGSGLRMGSDTPKQFMELDGLPVLMRTISVFLT
                     LRDIPEVVLVLPEAQITTWQRLCKKHHFETNLQIVPGGSTRFESVKNGLELLPADDSL
                     VAIHDGVRPLVTHEVVERCYAMAARGVCAIPVVNPVESVMIASGGKFTPFPRNNVLLV
                     QTPQTFRSATIKHCYNQPYSELFTDDASVAAACGVELHTVEGNRENIKITTPADLLTA
                     RVLLQAMKTD"
BASE COUNT          981 a          967 c         1057 g         1099 t
ORIGIN      
        1 tggcccgctc acggcatagc ccgcatgagg aattctttat catcgccaaa agtttctcgg
       61 tggcgttacg ggtgctagcg ctggcttcaa catcattcag ctttctaacc tcatcctcgt
      121 atgtgctcct gtagagttgt tcttcaaggc tggcaaggtg gttggtgatc agctgaccct
      181 cgggtgtttg cctcataagg tcaggcttga atgcccccga gaagcggagt gaggcaatgt
      241 atgcctccat gtcggcagca gcaagcattg ggatggtgtc gagttttacc agggagttca
      301 ccagctcctt ggttgctttc aggttgtggt catcgtcgag gtatagcgag gcaaatccta
      361 gcacagactc atactctcgt ctaaagatat ttgcaaaggt gttgttcccc ctgtacttaa
      421 atcgcatctc ctcaaaggtc ttaaccagct gccggtagta ctgcatcacc ttgaaaaggc
      481 ttatggaaac ggcgtcgctg ttaccggttc ggtagttgag gttataggca tcgaggtaac
      541 tcttgaagtt ggcgtaaacg gacaggaagg cgatgaaatc cttctcctgc cggtaggttt
      601 cggcgtttat cagcccctgc cgatagtcgt tttcaaccct cagcttccgc gtttcgatgc
      661 tggcaaaggt tacctcatcg ccaccgattg gctccatgcg gtagagcgac ttgtagttaa
      721 ccaccgctat taccgggtag ttgtggtatg caagtagctc tttcagcgtt tggcgcccaa
      781 cctggccatg ccttacggtg tccataaagt tttgtataag agctgtgtta acttcaatgg
      841 gcttgctgtt ggaagtggtt aaaacgtata gcttcaagga atgaacgcgg gtatcaaaat
      901 ttttctgctc aaatagcctg atggtggagc taaacctata ctcctttttc ctggtattag
      961 cctcaataaa gctgccgaat tctcctataa tgcccatcac cgcatcggta ggggttttta
     1021 ttttggatag attttgcagc tgggtggcaa cgagcgagag catctggtcg cgatcgttgt
     1081 tggtaatggc tttaacgcta atctccgcat caatgtaatc gtgtgcggag tagaggggta
     1141 ggttgtctat tatccgcaca tggtcgatgt tgtcgctcac cagggtttgg taacggttgt
     1201 ccttgtctct cgatatctgg aagttgtaga gtgggtaggt gatgtcgttc tccccgaaaa
     1261 atttcacatt tttcagcttc gccgtgatga acaggtactc caggcgttcc tcctcgagct
     1321 tcgattttcg gcgtattttg ccatttccct tattaggaaa gttgagctcg ttgattaggt
     1381 tgctaaacct gtcgccgttg agcaggaaga tatcggttga caggtactgc cattcgcctg
     1441 taaagttgaa cctggaggtg tcggaaaata ctagccgagg ttctatatcg cgctgggcag
     1501 caaccatata gcccgataat gttaaaagaa gcaacccgag aaatcgtttt aacatcggca
     1561 tacgtgggtt tataacagca ttctgcttca tcatagctag cttcaaaatt aaatatttag
     1621 catagcgcat ttcatcggag cgttcaacca gcctgtttct tttgtccacc ctcagctcac
     1681 ccttcgggga gggaccacct ccttggttct gtcgaacatg tagcggtagg tgatatggat
     1741 tttacgtatt ttccctccta tctggctggc tacccttcgc atggtagggt taaagtcgcc
     1801 aatccagttc agctccagct ccttgtactg aaagtcaggt cttagtgccc tgttggcaaa
     1861 ctctgttacc agagcacctt ctacaccctt gccctgatgt tgggacacaa ttccaaaaat
     1921 caggccaatg gccttggtgc aaaccttctt aaccttcagc aggtataaaa atttgagctt
     1981 atgaaccaga ccaaactgcc cgttcaaatg cctaaccacc tggtttaaat cgggcaccat
     2041 gataaagaac ccgatgggtt cattgttgta gtaggcaaag tacatcagct cggggtctat
     2101 aattggtttc agggttttca tcagggccat ggcatgaatg tgcgacattt tttttacccc
     2161 ggtgtacctt ccccaggcgt tgttgtaaat tgtgcggaaa tcctcggcaa actttttaag
     2221 gttgcgtttg gaaatgatgg taacatggta ggcggggtta cgggctatgc gttccgcctt
     2281 ttcgcgtata attgggtcaa gcccttcggg attcaccgga cggtagtagg tgaactggtg
     2341 aaagtaaggc ttaaaaccgt agccctcaaa aagctcgcgg tagtagggag gatggtagtc
     2401 catgcagtag tttggtgggt agtcgccctc aaccagcagc ccccaccaac ggtcgcgatc
     2461 gccaaagttg atagggccgt ccatggcctc cattcctctt tgctgcagcc actcctttgc
     2521 ggtgtcgaat agctgtgtgg cagcttcggg gctgttgatg cactcgaaaa atccaacccc
     2581 gccagttggc tgctcgttgt tgcgggctat ctcgtagtca ataaaagcgg caatccgtcc
     2641 aacaggcttg ttatcggagt atagcaccca tcgaatggct tcgccatgcc tgaactgctt
     2701 gttggttttt gggttgaaaa cgctttcaat atcgttgttg agcggcctga tccagctggg
     2761 ataatcgcgg taaagctcta ccggaaggtt taaaaaatct ttacgggttt ttcggttgtc
     2821 aacctctttc aatacaaaac tcatggcaca atcggttagc tatggctatt aatcggtttt
     2881 catggcttgt agcaggaccc gggcggtaag caaatcggcc ggtgtggtta ttttgatatt
     2941 ttccctgttt ccctccacgg tatggagctc aaccccgcag gcagcagcaa ccgaggcatc
     3001 gtcggtaaac aactcgctgt aaggctggtt gtagcaatgc tttatggttg ctgagcgaaa
     3061 agtctggggt gtttgcacca gcaaaacgtt gttgcggggg aatggggtaa acttgccgcc
     3121 gcttgcaatc attaccgatt caacaggatt cacaaccgga atggcgcaca cccccctggc
     3181 agccatggcg tagcaccgct caaccacctc atgtgtaacc agcggacgaa cgccgtcgtg
     3241 aatggccacc agcgagtcgt ctgcgggcag cagctccaaa ccgtttttta ccgactcaaa
     3301 gcgggtgcta ccccccggaa caatttgcag gttggtttca aaatggtgct ttttgcaaag
     3361 cctttgccat gttgtgattt gagcttcggg aagcaccagc acaacctcgg gtatatccct
     3421 cagggtgagg aaaacgctaa tagtgcgcat cagcaccggc aacccatcca gctccatgaa
     3481 ctgctttggg gtgtcggagc ccatacgtaa gccggagcca gcggcaacaa ccagggcaaa
     3541 acgcttgggc tgcattttta tggcaatttg atggcgaaag ttagccattt ttgactaaat
     3601 cataccagcc tgggagattt ttggttcaaa gggctacagg aacggatcca gctgggctga
     3661 agagttgagc ccatttccga ccagccggcc ggaatgctga agggagcata gtgcgagctt
     3721 agaaaggcca cgatggaaac ccgaaaatca accctgccac cctgcgctgg ttgctccggg
     3781 catttggttg aagcgggaac aggggtgggc tacatccaag agccacccgg gctcagcagt
     3841 ttaagaatca cgtagattga tgcatgcagc agctggaaat gcttacagaa ggttagcagc
     3901 tcatttgagg aaacgtaaaa aagttcataa gtttggttta tcaaatgaaa agcttttgta
     3961 attttgaata gaaagataaa acgcaacatt atagcgccta tattttcaaa tatggaaata
     4021 aggcgctatg gagtggcggg tataaactag ttgccgccaa gtgtaaaaag acaatcgaca
     4081 gaaccaaaat agaaagtaaa ccat
//
LOCUS       DAOOQX010000095         4094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_25960_length_4094_cov_2.963110, whole genome shotgun sequence.
ACCESSION   DAOOQX010000095 DAOOQX010000000
VERSION     DAOOQX010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25960_length_4094_cov_2.963110"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..1731
                     /locus_tag="PLX53_02375"
     CDS             205..1731
                     /locus_tag="PLX53_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71955.1"
                     /translation="MIKPFKTLLFLLSVFIVFLGISFFFPSGKKNIAKITLYVPSTDS
                     LLGAITSKPAMAEQPEANKKPPIMLNAEVDSNKTVLPTPENGEFKKSMMKTDTLTRNT
                     SLAYSTNSTATDKIVPIEFTTEALGSFFAALKNGEAGSSQVRVLHFGDSQVEGDRISD
                     YLRSVLQEKFGGYGPGILPAYPQNYQPYGIRQSASKGWNYSNLLQPNEMVRKYGILGG
                     LSETNDKDATEYIRFKKNARDARNALFDRIRVFYGKNDGPCMLQLLKDGEVADTEILQ
                     QAQTLAQHSFTISPDAKTVELQLANTGHILIYGLSLESERGIYVDNIPLRGSSGEIFT
                     GFDHELARQLFGMLNVKLVILQFGLNIAPGNLTSYDYYEERLYKQVMAIKSFKPDVSV
                     LIIGISDMLRRVDGRLETYPSIEKIRDAQRSAAFRSGAAFWDCYRAMGGKNSMLTWVN
                     SNPPLGSMDYAHFTYRGARVIAGKLTKSLLEAFDRYSFPESSAIPTEAIARPDSSQGG
                     "
     gene            1818..3347
                     /locus_tag="PLX53_02380"
     CDS             1818..3347
                     /locus_tag="PLX53_02380"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOY71956.1"
                     /translation="MGSWTNILSSIFSYTEGNPLLFTEMSFWVFFAVLLTGYSFIYNR
                     PLARSAYLLAFSLYFYYKSSGLFFFLLIFSTVCDYTIGLLIPRYSSKTARKLLVALSV
                     IINLAVLSYFKYAYFITETVNQLFGLNLEVVNYLSGWANGIFGSHFDTSTIILPAGIS
                     FYTFQTISYTVDVYRRKVAPVKNIIDFGFYVSFFPQLVAGPIVRAAEFIPQLYRKFSL
                     TYREFGHALFLIIAGLVKKMVISDYLSVNFVDRVFDNPLSFSGFENLMATYGYALQIY
                     CDFSGYTDIAIGVALLLGFRLPPNFNSPYKAVSIADFWHRWHISLSTWLRDYLYIPLG
                     GSRKGKVRTHLNLMVTMLLGGLWHGANLKFVVWGGIHGVSLVLHKLWTSHSPRVKRAD
                     SWWRRAVGAFFTFQLVSFSWIFFRSSTLSSAFDMLGQIFTRFGTPSIPQMLVSYAKPF
                     ALMLAGFAIHWLPFRVSEGVRGWFIETTVLVKLLITVMAVLLILQLNSAELQPFIYFR
                     F"
     gene            complement(3722..>4094)
                     /locus_tag="PLX53_02385"
     CDS             complement(3722..>4094)
                     /locus_tag="PLX53_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOY71957.1"
                     /translation="ANIDLKSFSNDIYLKLSAGTLQPVLNTLKTLNEIGVWLEITNLV
                     IPSWTDDMDMIRRMCGWLVENGFSENPLHFSRFFPLYKLTQLPQTPVSTLIRARDIAL
                     KAGLKHVYIGNVPSMDASNTT"
BASE COUNT         1059 a         1032 c          913 g         1090 t
ORIGIN      
        1 actcaaatac tctaacccct ttctgctctt tgcacatcga gggttatggt atattttcag
       61 cataaaatat tttttttgaa agttacaaaa aaatggcgat ggttttactc gctataattc
      121 ttatatgtaa aattagcaat tttacaaaat taccttactt ttatacgggt tttacagaaa
      181 aaataccact aaaagctacg ataaatgatt aagcctttta aaaccctttt gtttctgcta
      241 tcggttttca tcgtgtttct aggaatttcg tttttcttcc caagcggaaa gaaaaacatt
      301 gccaaaatca ccctgtatgt tccttccacc gacagcctgc ttggggccat cacctcaaaa
      361 ccagcaatgg cagaacaacc agaagcaaac aagaaaccac ccataatgct caatgctgaa
      421 gtagattcaa ataaaaccgt gctacccacc cctgaaaatg gcgaattcaa aaaatcaatg
      481 atgaaaaccg acacgttgac aaggaatacg tcgttggcat actccacaaa cagcactgct
      541 accgacaaaa ttgttcctat tgaatttact accgaggccc tgggtagctt cttcgccgct
      601 ttaaaaaatg gggaggccgg cagctcccag gttagggttc tacactttgg cgactcacag
      661 gttgagggcg atcggatttc tgactattta cgttcggtgt tacaggaaaa gtttggtggt
      721 tacggcccag gcattcttcc tgcctaccca caaaactacc aaccttacgg aatcaggcaa
      781 tcggcgtcaa aagggtggaa ttattccaac cttctccaac ccaacgagat ggtgaggaaa
      841 tatggtatcc tgggtggatt atcggagact aacgataagg atgctaccga gtacattcgc
      901 ttcaagaaaa atgcaaggga tgccagaaat gccctgttcg ataggataag agtattctat
      961 ggaaaaaatg atggtccctg tatgctgcag cttctgaaag atggagaggt ggctgatacc
     1021 gaaatacttc aacaagctca aacactggca cagcattcct tcacaattag ccccgatgcc
     1081 aaaacagttg aacttcagct cgccaacacg ggtcatatcc tgatatatgg tctatcgctt
     1141 gaatcggagc ggggtattta cgttgacaat attcccctaa ggggaagctc gggcgaaatt
     1201 tttacgggat tcgaccatga actggccagg cagctgttcg gtatgcttaa cgtgaagctg
     1261 gttatcctgc agttcggttt aaatattgcc ccgggcaacc taaccagcta cgactactac
     1321 gaagagcgtc tctacaagca ggtaatggcc atcaaatcgt ttaaacccga tgtttccgtt
     1381 ctcatcatcg gcatttcgga tatgctgcgc agggttgatg gccggctcga aacctatccc
     1441 agcatcgaga agatcaggga tgctcaacgg agtgcggctt tcaggtcggg tgcggctttc
     1501 tgggattgct accgggcaat gggagggaaa aactccatgc tcacatgggt caattccaat
     1561 cctccgcttg ggtcaatgga ttacgctcac ttcacctaca ggggggccag ggtgattgcc
     1621 gggaaactta caaaatcgct gcttgaagcc tttgataggt atagtttccc cgaaagctcc
     1681 gctataccaa ccgaagcaat tgccagaccc gacagtagcc agggtggttg agctgaataa
     1741 tctgtcagca ttttgccata agaaaaaaat caccacgggt ggaagatatc aaaaacgatt
     1801 agacctaaaa gaaaagaatg ggatcatgga ccaacatact gtcaagcatc ttcagctaca
     1861 ctgagggcaa tcccctgcta tttaccgaga tgtcgttctg ggtgtttttt gccgtactgc
     1921 ttacgggtta ctctttcatc tacaaccggc cgttggcccg cagcgcatac ctgcttgcgt
     1981 tcagccttta cttctactac aaatcgagtg gattgttttt cttcctgctc atcttctcaa
     2041 cagtatgcga ctacaccatt gggctactca ttccccgcta cagctcaaaa accgcccgta
     2101 agctgctggt ggcgctaagc gttatcatca accttgccgt gctctcctac ttcaagtatg
     2161 cctacttcat caccgaaacg gttaaccagc tgtttggctt aaaccttgaa gtggtcaact
     2221 acctttcggg gtgggcaaac ggaatttttg gttcccactt cgacacctca accatcatcc
     2281 tgccagccgg catttcgttc tacacattcc aaaccatcag ctacacggtt gacgtttacc
     2341 gccgcaaggt ggctcctgtt aagaacatca ttgactttgg cttttatgtt tcgttctttc
     2401 cccagctggt ggccggcccc attgtgaggg cagccgagtt tatcccccag ctctacagaa
     2461 agtttagcct gacctacagg gagtttggtc atgcgctttt cctaattatt gcagggttgg
     2521 tgaaaaaaat ggtcatatcg gactaccttt cggtcaactt tgtggatagg gttttcgata
     2581 acccgcttag cttctcggga tttgaaaatc tgatggccac ctacggctat gccctgcaga
     2641 tatactgcga tttctcggga tacaccgata ttgccatcgg ggttgccctt ttactaggct
     2701 tcagactccc gccaaacttc aactcgccct ataaagcagt cagcattgcc gacttctggc
     2761 ataggtggca catctcgctg tccacatggc ttcgcgacta cctctacatt ccgcttggcg
     2821 gtagccgtaa gggaaaggtc agaacacacc tcaacctgat ggttaccatg ctgctgggtg
     2881 ggttatggca tggtgccaac ctgaagtttg tggtttgggg cggcatccac ggggttagcc
     2941 ttgttctgca taagctttgg acttcacatt cgcccagagt caaacgtgct gacagctggt
     3001 ggagaagggc ggttggcgca ttttttacct tccagctggt atcattctcc tggatattct
     3061 tccgctcttc aacgcttagc tccgccttcg acatgctagg ccaaatattc acccgatttg
     3121 gcaccccctc cattccgcag atgctggtat cctatgccaa accttttgcc ctgatgcttg
     3181 caggctttgc cattcattgg ctgccgttca gggtaagcga aggggtaagg ggttggttca
     3241 tcgaaactac agttctcgta aaactactga ttactgttat ggctgtgctg ctcatcctcc
     3301 agctcaattc agcagagctt cagccgttca tctacttcag gttttaaaat gatggtatct
     3361 tgttatatat cgatgataaa tggatgaatg aaaacataag ctgctcccct caacaggatc
     3421 aaaatgcatc cccaagtaca cttaggacga tttagaaaaa ttccgccagc ttaacaccat
     3481 actggcactc agcctcaagt ttggacccat gctccagctc aaggctgttt ataacaaacc
     3541 ttccggcaac tatcaataaa ccaccgctca aaggcagcta cttccgattg ggaaatgcgc
     3601 tggatgttca ctcccacaca cctttcacca ttgagccgca gtatgcgcac ttcccgtttt
     3661 taaggttgat gctgtaaatg gaaaaccccc tgcgctccaa aacaaccttg ccgcaatttg
     3721 gtcaggtagt gtttgaggca tccatgctgg gcacattgcc aatgtaaaca tgctttaacc
     3781 ccgccttcag ggcaatatcc ctggccctta tcagcgttga aacaggggtt tgaggaagct
     3841 gggtgagctt gtaaagcgga aaaaaccggc tgaaatgaag cgggttttcc gaaaatccat
     3901 tttcaacgag ccagccgcac atccgcctga tcatgtccat atcgtcggtc cacgacggga
     3961 tcaccaaatt ggtaatttcg agccagacgc caatttcgtt aagagttttc aaggtgttca
     4021 gcaccggttg aagcgttccc gccgaaagct tcaggtaaat gtcgttgctg aacgatttca
     4081 ggtcgatgtt ggca
//
LOCUS       DAOOQX010000096         4077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_26119_length_4077_cov_2.843113, whole genome shotgun sequence.
ACCESSION   DAOOQX010000096 DAOOQX010000000
VERSION     DAOOQX010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26119_length_4077_cov_2.843113"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="PLX53_02390"
     CDS             <1..1237
                     /locus_tag="PLX53_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOY71958.1"
                     /translation="IVIPIVALEELDYFKKGNEMVNYNAREFVRELDKITGDNLFNGG
                     VSLGKNLGKLRIETGKPMPKAMLDSFAEATPDHRILAITYHLASVNPDRPVILVTKDI
                     NLRMKAKSLGIVAQDYLTDKVQDIDSLKKEIEVVDGVDDELIQKLYDNPSELSIDQLK
                     VKPYANQYLILKGNKSSALAHFDKATNMLNRVEKTRAYGIEPRNSEQTFALDALLRSE
                     VKLVALTGKAGTGKTLLALAAALQQEENFNQILLARPIVALSNKDIGFLPGDINEKIS
                     PYMQPLFDNLSVIKNRFKPASKEYMHIEEMLRTEKLVITPLAYIRGRSLSNVYFIVDE
                     AQNLTPHEIKTIITRAGEGTKFVFTGDIFQIDHPYLDMKSNGLTYLSDKMHGQELFAH
                     INLVKGERSYLAELASNLL"
     gene            1345..1830
                     /locus_tag="PLX53_02395"
     CDS             1345..1830
                     /locus_tag="PLX53_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOY71959.1"
                     /translation="MKRDLYLLRHAKSVERGSEMRDADRALKPRGIEDVLNLAGRVHA
                     KAKSIERILSSPANRTIHTAILFAEATGIPMDRIAICSDIYLADDDDLALIVSEADNQ
                     LTSLMIVGHNPSVTNLANRFLNEPVFDVPTSGLVVLQFDADSWMNISKSTLTDSFTVF
                     P"
     gene            1946..4045
                     /gene="ppk1"
                     /locus_tag="PLX53_02400"
     CDS             1946..4045
                     /gene="ppk1"
                     /locus_tag="PLX53_02400"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOY71960.1"
                     /translation="MGRKNLPLVNREISWLSFNGRVLQEAEDKNVPLIERLKFLGIFS
                     NNLDEFFRIRVATLRRLLVVEKGAKKVMGDNPRKILEKIQRLVVSYQEKFDSIYSDVK
                     LQLRAEGIYIMDETELDPDQVDFVRSLFKRKISMSLGPIMISRLDRFPQLVDQSIYLA
                     VKLSSSENRQAKEYSLIEIPTRNISRFIRLPRKDNQEYIILLDDVIRLCLDMVYSILP
                     YDRFEAYTIKITRDAELDVDNDISESLLDKISKGVKNRKRGEPVRLVYDEKIAPDLLN
                     FIVKGLSLDEKDYILPGGRYHNFKDFMDFPNFGRSDLVNAPMKPVQVAELENASSILG
                     VMAKHDFILHYPFHDFAYYVKMLREAAIDPKVTAIYITLYRMAAESRVVNALMNAAQN
                     GKKVTAVIELRARFDEHANIYWAKQMQEAGVNVVFGMPGIKVHSKMTLIIRHEGRKDV
                     RYAVVSTGNFHEGNANLYTDVNLFTVNQGIAREVEKVFTFLEFNYKTFSYKHLLVSPV
                     NMRRKLYSLIENEMDNAAKKLPAYIHCKINNLVDTEMIAKLYQASQAGVEIKLVVRGI
                     CSLVPGVKGVSDNIEVFSIVDRLLEHSRIFIFANSGKETCYISSADWMTRNLDYRVEI
                     ATPIYDKHLISELKTVINMALKDNVKSRVIDQKQSNSFRNPEAGEEPFRSQLELYNYY
                     KSKENLYNSISEDNNLT"
BASE COUNT         1156 a          918 c          935 g         1068 t
ORIGIN      
        1 cattgtaata cccatagttg cgctggagga gctggactac tttaaaaagg ggaatgaaat
       61 ggttaactac aacgcccgtg agtttgttcg tgagctggat aaaatcacag gtgataacct
      121 attcaacgga ggagtttccc tggggaagaa tctcggcaag ctacggattg aaacagggaa
      181 acccatgcct aaagccatgc tcgactcctt tgccgaggct actcccgacc accgaatact
      241 ggccattacc taccatttgg catctgttaa tccagacagg ccggttattc tggttaccaa
      301 ggatattaac ctgaggatga aggcaaaatc gttgggaatt gttgctcagg attacttaac
      361 cgataaggtt caggatattg actccctgaa aaaggagatt gaagtagttg acggggttga
      421 cgatgagctg attcaaaagc tatacgataa ccccagtgag cttagcatag accagcttaa
      481 ggtaaaaccc tacgccaacc agtaccttat tcttaagggt aataaatcga gcgcactagc
      541 gcatttcgac aaggccacca acatgctgaa cagggttgaa aaaacgcgtg cttacggcat
      601 tgaaccccgc aactccgagc aaacttttgc cctagatgcc ttgttacgtt cggaagttaa
      661 gctggtggcc cttacaggaa aagccggcac aggaaaaacc ctgctagccc tggctgcagc
      721 tcttcagcag gaggaaaact tcaaccaaat acttctggcc aggcccatag ttgccctctc
      781 gaataaagat atcggtttcc tgcctggcga catcaacgag aaaatcagcc catacatgca
      841 gccacttttc gataaccttt cggtgattaa aaataggttc aaacctgcca gcaaggagta
      901 catgcatatt gaggaaatgc ttcgcaccga gaaacttgtc atcaccccgt tggcctacat
      961 taggggacga agcctgtcaa acgtgtactt catcgttgac gaggcgcaaa accttactcc
     1021 ccacgagatt aaaaccataa ttacccgggc aggggaggga actaaatttg ttttcaccgg
     1081 cgatatattt cagattgacc acccctacct ggacatgaag tcgaacgggc taacctacct
     1141 ttccgacaaa atgcacgggc aggagctgtt tgcccacatc aacctggtta agggcgagcg
     1201 aagctacctt gccgagctgg ccagcaacct gttgtaatag atttgcccac acttcagaag
     1261 caactttccg ggtatcaact tctgaaatca gctgggcatc cttacatgta attgagagta
     1321 ggtttattgt gaaagtaatg aggtatgaaa agggatttgt acttgcttcg acacgccaag
     1381 tcggtagaaa gagggtccga aatgagggac gctgatagag cactgaaacc taggggaatt
     1441 gaagatgttc ttaaccttgc aggaagagtt catgcaaagg ccaagagcat tgagcgcata
     1501 ctttcgagcc ctgctaaccg taccattcac acggctatac tatttgccga agcaacgggc
     1561 atacccatgg acaggattgc tatctgttcc gatatctatc ttgccgacga tgatgatctg
     1621 gcgcttattg tttctgaagc tgataaccag cttacctcat tgatgattgt ggggcataac
     1681 ccttcggtta ccaacctggc caaccgattt ttaaatgaac ctgtttttga tgttccaaca
     1741 tccggccttg tcgttcttca gtttgatgca gatagctgga tgaatatttc gaaatcgaca
     1801 ctaaccgata gtttcaccgt atttccctga tacagggtta atttgctacg atatcagcat
     1861 aaaccttaac atttagataa catttcgttt acgcattgtt cgaaaaatac ttcttaaaat
     1921 tgaacttttt aacctaaact aagctatggg ccgaaaaaac ttgcccttgg taaacaggga
     1981 gattagctgg ctttcattca acggacgggt gctccaggag gcagaggata aaaatgtgcc
     2041 attgatagaa agattgaaat ttctaggcat cttctccaat aacctcgatg agttttttcg
     2101 aatccgcgtg gccacgcttc ggcgtttgct ggtcgttgaa aagggtgcaa aaaaagttat
     2161 gggtgataac cccagaaaaa tactagaaaa gatacaacgc ctggtggttt cgtatcagga
     2221 aaagtttgat tctatctata gcgatgttaa gctacagctg agggctgagg gtatttacat
     2281 tatggatgag acggagctcg accccgatca ggttgatttt gttcgctccc tgtttaaacg
     2341 aaaaatctcc atgtcgcttg gtccaatcat gataagccgg cttgaccgtt ttccccagct
     2401 ggttgatcag tctatttacc ttgctgttaa gctctctagt agcgaaaacc gtcaggccaa
     2461 ggagtactcc cttattgaga ttcctacccg taacatttct aggttcattc gcctaccccg
     2521 caaggataac caggagtaca ttatcctgct cgacgacgta attaggttgt gcctggatat
     2581 ggtttacagc atcctgccct atgataggtt tgaagcctac accatcaaaa ttacccgcga
     2641 tgctgagctg gatgttgaca atgacatatc ggagagcttg ctggataaga tttctaaagg
     2701 tgttaagaat aggaagcgtg gcgaaccggt gaggctggtt tacgacgaaa agattgcccc
     2761 tgatttgctc aatttcatcg tgaagggttt atcattggac gagaaggact acattttgcc
     2821 cggtggtcgc taccacaact ttaaggactt tatggatttt ccaaattttg gacgctccga
     2881 cctcgtcaat gcccctatga aaccagtgca ggttgccgaa cttgaaaatg ccagcagcat
     2941 tcttggagta atggccaagc acgactttat cctgcactac cccttccatg attttgccta
     3001 ctacgttaaa atgctccgtg aggctgctat tgatcccaag gtaactgcta tttatattac
     3061 cctataccgc atggctgccg aatcgcgggt ggttaacgct ttgatgaacg ctgcccaaaa
     3121 tggcaagaag gttaccgctg ttatcgagct tcgcgcccgt tttgacgaac atgccaacat
     3181 ttactgggct aagcaaatgc aggaggccgg cgttaacgtg gtgtttggaa tgccgggcat
     3241 taaggttcac tctaaaatga ccctgattat ccggcacgaa ggtcgaaagg atgttaggta
     3301 tgctgttgtt agcacgggta actttcatga gggtaacgcc aacctctaca ccgacgttaa
     3361 cctttttact gttaaccagg gcatcgccag ggaggttgaa aaggttttca cctttctgga
     3421 atttaactac aagacattta gctacaaaca cctgcttgtt tcacctgtaa atatgcgtcg
     3481 gaagctgtac tcccttatcg aaaacgagat ggataatgct gccaaaaaac taccggccta
     3541 catccactgc aaaatcaaca acctggttga taccgaaatg attgccaagc tctaccaagc
     3601 tagtcaggcc ggggttgaaa ttaagctcgt ggttaggggg atttgctccc ttgttccggg
     3661 ggtaaaaggg gtgagcgata atattgaggt tttcagcatt gttgacaggc tgctggagca
     3721 ttcccgaatt tttatttttg ctaatagtgg aaaggagacc tgctacattt catctgccga
     3781 ctggatgaca cgtaatctcg actaccgggt tgaaatagca acacctattt acgacaaaca
     3841 tttaatttcc gagcttaaaa ccgtaataaa catggcgtta aaggataatg ttaagtcaag
     3901 ggttatcgat cagaaacaaa gcaactcctt tagaaaccct gaggcgggag aagaaccatt
     3961 tcgctcacag cttgaacttt acaactatta caagtcgaag gaaaatctgt ataactccat
     4021 ttcggaagat aataacctga cataagaatg gtaattcctg gtaaatattt caataag
//
LOCUS       DAOOQX010000097         4053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_26327_length_4053_cov_3.723612, whole genome shotgun sequence.
ACCESSION   DAOOQX010000097 DAOOQX010000000
VERSION     DAOOQX010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26327_length_4053_cov_3.723612"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLX53_02405"
     CDS             complement(<1..243)
                     /locus_tag="PLX53_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71961.1"
                     /translation="MEKGAKGNITGNQLEVAVKTVLTGKGFELINYRVWDKNKEKYGE
                     ELLLENVPFTTIYNHKGNTEFLLISKRFNLQIRIECK"
     gene            complement(244..1233)
                     /locus_tag="PLX53_02410"
     CDS             complement(244..1233)
                     /locus_tag="PLX53_02410"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HOY71962.1"
                     /translation="MTQAIKYKIKDDNVSTSEDLFNHFNVGAKPFLKWAGGKGQLLDK
                     FQEFYPKLLKQKEIKTFYEPFLGSGAVFFDIAQHYDIEDAYLYDINEELILTYKVIQK
                     DVTKLLDFLYQYQKKYIKLDKKQRQVFFYDQRANFNLQRSNINFDKYSEQWFPRAAQL
                     IFLNRTCYNGLYRVNSKGEFNSPAGDYDNPTICDEQNLLAVHQVLKIAVIKKADFKEI
                     INDIKAKSFVYFDPPYRPISKTASFKAYSKQDFAENEQIQLAKLFRQLDNKGTHLMLS
                     NSDPKNYDPSDNFFDDIYRGYNITRIPAKRMINSDPTKRGAVNELVVTNYQIA"
     gene            complement(1527..2366)
                     /gene="prmA"
                     /locus_tag="PLX53_02415"
     CDS             complement(1527..2366)
                     /gene="prmA"
                     /locus_tag="PLX53_02415"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOY71963.1"
                     /translation="MAAFTELRIKTSPFSEETNEIIIAQLAELGFDSFMESESELLAY
                     SSIHSVSAAINTLHSIAIPAGVSIDYSAGETGERDWNEEWETGFEPVTVDGRCHIRAS
                     HHPVNPAAEYNITINPRMAFGTGHNPTTYMMLEALMDMNVEDCRVLDMGCGTGVLAIM
                     AELRKARSVTAIDTDEWAYRNTLENIRINGCSRVEVLLGDAQLLANREFDLVLANITL
                     NVLLANLPAYAQSLVCKGHMLLSGILTTDVDRLLQYAKSIGLEPVATRTREGWAMVQL
                     HRS"
     gene            complement(2366..3121)
                     /gene="tpiA"
                     /locus_tag="PLX53_02420"
     CDS             complement(2366..3121)
                     /gene="tpiA"
                     /locus_tag="PLX53_02420"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOY71964.1"
                     /translation="MRKKIVAGNWKMNTSLAEGFSLAKEVIAGSSRVDQGVELVVIPP
                     FTHLSEIVKLARGSRVRVGAQNCAQWPKGAYTGEVSASMLQSAGVEYVILGHSERREY
                     FNETNRSLFEKLQLALQENLTPIFCCGEKLGEREAGQHFAVVEKQVAESLFGLETRQV
                     QQVVIAYEPVWAIGTGKTATADQAQEMHAFIRGLLAGKYGSTLADSISILYGGSCKPS
                     NTAELFAKPDVDGGLIGGASLVAADFIAIAGSF"
     gene            complement(3185..>4053)
                     /locus_tag="PLX53_02425"
     CDS             complement(3185..>4053)
                     /locus_tag="PLX53_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY71965.1"
                     /translation="SPLSVHNPNNPKSYIYLDTLLYSGIVNLVKLSDNRQLVLKLPYL
                     RRSYQSRKPVTSIIAETLPNTFILALVAIIFATIVGIAMGIIAAIRKDTAVDRMLLIT
                     SSFGMSLPSFFAAILVGWVFAYLLGSYTGLNLTGNLWEVDDLGEGVHLQLKNLILPAF
                     TLGIRPLAEITQLMRSAMLDTLNQDYIRTARAKGLKFRHIIMRHALKNSLNPVLTAIS
                     GWFASLMAGVIFVEYIFGWKGLGFVMVTALNSYDVPLVIGCVISISLIFGVVNLIMDI
                     IYSLLDPRVRMG"
BASE COUNT         1028 a          917 c          931 g         1177 t
ORIGIN      
        1 cttgcactca atacgaattt gcaaattgaa tctctttgaa atgagtagaa attctgtatt
       61 gcctttgtga ttgtaaattg ttgtaaatgg aacattctca agtagcagct cttctccgta
      121 tttctctttg tttttatccc aaacacgata gttgataagt tcaaatcctt ttcctgtaag
      181 aacagtttta actgccactt caagttggtt tccagtaata ttacccttag ctcctttttc
      241 catttatgca atttgatagt ttgtaacaac gagttcatta actgctccac gctttgtcgg
      301 gtcagaattt atcattcgtt tagcaggaat tctagtaatg ttgtagccac ggtatatatc
      361 gtcaaaaaaa ttgtcagaag ggtcgtagtt tttgggatct gagttgctta acattagatg
      421 cgttccttta ttgtcaagtt gcctaaatag ttttgcaagt tgaatttgct cattttctgc
      481 aaagtcttgt ttactgtatg ctttgaaact agctgtttta cttattggcc tgtaaggcgg
      541 atcaaaataa acgaatgatt ttgctttaat gtcgtttata atttctttga agtcagcttt
      601 cttgattaca gcaattttca aaacttgatg aactgctaat aaattttgct cgtcacaaat
      661 tgtagggtta tcatagtcac ctgctggaga attgaactct ccttttgagt ttactctgta
      721 aagaccgttg tagcaggttc tgtttaaaaa tattagttgt gctgctctag gaaaccattg
      781 ttctgaatac ttatcaaagt tgatattgga gcgttgtaaa ttgaaatttg ccctttggtc
      841 atagaaaaat acttgacgtt gcttcttatc gagtttgatg tatttctttt gatattgata
      901 aagaaagtca agaagttttg ttacatcctt ttgaatgact ttgtaagtca aaatcaattc
      961 ttcgttgatg tcatataggt aagcatcttc aatatcataa tgctgtgcaa tgtcaaaaaa
     1021 aactgcaccg ctgcccaaaa aaggttcata aaaagtttta atctcttttt gtttaagtag
     1081 ctttgggtag aattcttgga atttgtcgag tagctgtccc tttcctccag cccattttag
     1141 aaaaggcttt gccccaacat tgaaatggtt gaaaaggtct tcagatgtcg aaacgttgtc
     1201 gtccttaatt ttatatttta tagcttgcgt catagtgttt aaaaccattg tttaaattta
     1261 gtcagaaaaa agagtaaagt aaaaaaattg agattatagt ttcgaccaag cattttgcac
     1321 tactcgttta tccccgccct tcatatggca ttacttccaa cttgggaagt atagacgcta
     1381 cgacattaca ttatttggta aacaaattta tggacttttc ttaaatcaca aaaaatctaa
     1441 aatatttcga cagatacctt ctccgatggc ctgagcatgt gtctcgaacc tgtagctgag
     1501 gttaatgggt ggttggtgta gttaacctac gaccggtgca gctgcaccat tgcccagccc
     1561 tcgcgggttc gggttgcaac gggctctagt cctatgcttt tagcatattg cagaagccta
     1621 tctacgtcgg tggtgagtat gccgctaagg agcatatgtc ccttgcagac caggctctga
     1681 gcgtaggccg gcaggttggc aagcagcacg ttcagggtta tgttggccaa aaccaggtcg
     1741 aattcccggt tggccagcag ctgcgcatcg ccgagcagca cctctactct ggagcagcca
     1801 ttaattctga tgttctccag ggtgttacgg taggcccact cgtcggtgtc gatggctgtg
     1861 acggaacggg cttttctcag ttcggccatt atggccagca cccctgtacc gcaccccata
     1921 tctagcaccc ggcaatcctc tacattcatg tccatgagcg cttcaagcat catgtaggtg
     1981 gtggggttgt gccccgtgcc gaatgccatc ctggggttaa tggttatgtt gtattcagct
     2041 gcggggttta ccgggtgatg tgaagccctg atgtggcacc ggccatcaac agttaccggt
     2101 tcgaagcccg tctcccattc ctcgttccag tccctttcgc cggtttcacc tgcggagtag
     2161 tcaatgctca cccctgccgg aatcgctatg gagtgaaggg tgttaatagc agcgctaaca
     2221 ctatgaatac tagaatacgc tagtagttca ctttcgctct ccataaagct gtcaaagccc
     2281 agctcggcca gctgggcgat gattatctca ttggtttcct ccgaaaaggg agaggtttta
     2341 atgcggagct ccgtgaaggc agccatcaga acgagccagc tatggctata aaatcagcag
     2401 caacaagcga tgcgcctcca atcagcccgc catccacatc gggcttggca aaaagctcgg
     2461 cggtgttcga gggcttgcag ctacccccgt acagtatgct aatgctatcg gccagtgttg
     2521 aaccgtattt gccggccagc aggccacgta tgaatgcatg catttcctgc gcctggtcag
     2581 ccgttgcggt ttttcctgtg ccaatggccc aaacgggctc gtaggcaatt accacctgct
     2641 gaacctgcct ggtttccagc ccgaaaagcg attcggcaac ctgcttttcg acaactgcaa
     2701 agtgctgtcc ggcttcacgt tctcccagct tttcgccgca gcagaagata ggggtgaggt
     2761 tttcctgaag cgctagctgc aacttttcaa aaagcgagcg gttggtctcg ttaaagtact
     2821 ccctgcgttc ggaatgccca agaataacat attcaacccc agccgattgc agcatggagg
     2881 ccgatacctc accggtgtag gcaccctttg gccattgggc acagttttga gcacccaccc
     2941 ttacacggct gcccctggcc agctttacaa tctcgctcag gtgggtgaac ggaggtatca
     3001 ccaccagctc aaccccctgg tcaaccctcg atgagcctgc aatcacctcc ttggccaggc
     3061 tgaatccttc tgccaggctg gtgttcattt tccagtttcc cgctacaatt ttttttctca
     3121 tggcatatta tttttaaggt ttcccgtcaa cagaatttcc agacaaacat agcaaaaatt
     3181 cctgtcagcc cattcttacc cttgggtcaa gcagggagta gataatatcc atgatgaggt
     3241 tgaccacacc gaaaattaag gagatggata ttacgcatcc gatgaccagt ggaacgtcgt
     3301 agctgtttaa ggctgtaacc ataacaaatc caagtccttt ccacccaaaa atatattcca
     3361 caaagatgac acctgccata agggaggcaa accatcccga aattgccgtt aaaacagggt
     3421 taagggagtt ttttagtgca tgtcgcatga tgatgtgccg gaactttagc cccttggccc
     3481 tggccgtgcg gatgtagtcc tggtttaggg tatcgagcat ggcgctacgc ataagctggg
     3541 tgatttctgc tagtggtcgg atacccagcg taaatgcagg tagaatgagg tttttaagct
     3601 gaagatgaac cccctcgccc aggtcatcaa cctcccaaag gttaccggta agattaagcc
     3661 cggtgtagct gccaagcagg taagcaaaaa cccaacctac cagtattgca gcaaaaaatg
     3721 agggcaacga catgccaaac gatgatgtta tcagcagcat cctgtcaacg gccgtatcct
     3781 tacgaatggc agcaataatt cccatggcaa tgcccacgat ggttgcaaat attatggcta
     3841 ccagtgccag tataaaagtg ttgggaaggg tttcggcaat aatggaggtt accggtttgc
     3901 ggctttggta agaacggcgc aggtagggga gtttgagtac gagctgtcgg ttgtcggaga
     3961 gttttaccag gttaacaatg ccgctgtaga gaagtgtatc gaggtatatg tagcttttgg
     4021 ggttgttggg attgtgaacc gacaggggcg aca
//
LOCUS       DAOOQX010000098         3999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_26852_length_3999_cov_2.654412, whole genome shotgun sequence.
ACCESSION   DAOOQX010000098 DAOOQX010000000
VERSION     DAOOQX010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26852_length_3999_cov_2.654412"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="PLX53_02430"
     CDS             <1..1270
                     /locus_tag="PLX53_02430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOY71966.1"
                     /translation="LLADDPNQYVTLNSDSPLDSKYYKESELTDEMGKWAFSAKVGES
                     YGPTFDGNSYKIIRLVNVKMLPDSVKARHILISPKAQNQEAVAKAKATADSLLNVIKR
                     GGSWDDLASHFSDDPGSKDKGGDLGWFPGGVMVKNFDEAAFNLSKNEVKVVETNYGFH
                     ILQVTDRGKESRKVQLATLERKVVPSSTTSQRIYSEASQFALSNRTYEQFKTAADAKK
                     LTRRVASNLLANDQNIAGLESPREVIRWAFKAEEGDVSNVFELANMYVVASLTAARSE
                     GVAPLKQVVADVRTKVIRDKKAEKLAARVSDALKGASDIFSVAGKLNGKVEHAENVTF
                     SSFTLPYAGFEPAVIGVASVSSEGKLQGPVNGNAGVYVLAVTSIVTDQGDAGMEKLRL
                     ANTYQSRAYYEAFEALKSKIGIVDKRYNFY"
     gene            1428..1802
                     /locus_tag="PLX53_02435"
     CDS             1428..1802
                     /locus_tag="PLX53_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOY71967.1"
                     /translation="MKVAVPTRNGVVDEHFGHCEAYTVFTIDSGNKIENSEILPSPQG
                     CGCHSNIAATLKEKGVEVMLAGNMGDGALNMLAGHGIQVFRGCSGDARRLVENFLSGN
                     VEDSGEGCHHHHEHEEGHTCNH"
     gene            complement(1966..3627)
                     /locus_tag="PLX53_02440"
     CDS             complement(1966..3627)
                     /locus_tag="PLX53_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOY71968.1"
                     /translation="MIKYTFLRFVKKLKHEKYLLFSIFIVLCTLLLFINVGTYGVVES
                     SDARYAEISREMLESGDYIHPTLLNIQHYHKPPLTYQLTALGYQIFGVNELGARFFLQ
                     LSLLIQLLLVYALSNILFGNRGTALWAAIIYFSFPLTLISTRNLTTDSFLATFVLLSI
                     YSWVKYRKYGKFRYLYLTAISLALGFCTKGPVVLLVPLVFIIAYNRIEKSKNRFGVHH
                     LLSLVVFLGISLWWFVFLIRENGAFADYFLGRQTVERFGKDVFNRSEPFWYFVAFAPL
                     VGLPWLLILPYLMVQNRKSILKKGINSVLTLSFVIPLVFFSISTSKRILYILPFYSIL
                     AMLTANLMANLISSKEKVIDRSVTIFSMIVLLVATATMFIHTNYVIPKGVGIASILLI
                     VVILAIYRYLQGGRKFKPVLVSFLVSGFLLITSGLLMQKNELEFNISRPISNFIKSKN
                     LQDRNILIYNTMKPSIAFDMRKPVISLYDGSNTLAREVQFEENSAWKSYFYDLTDSID
                     TNRLKGLMERPSVLVSYKHPLPENRQWLAKRFSTRKDIGKWHIYY"
BASE COUNT         1170 a          779 c          956 g         1094 t
ORIGIN      
        1 tctactcgct gacgatccaa accagtatgt taccctcaac tccgatagtc cactcgactc
       61 gaagtactac aaggaaagtg agctgactga tgagatggga aaatgggctt tttctgccaa
      121 ggtaggcgaa agctacggcc ctacatttga cggaaattcc tataaaatta tccggctggt
      181 gaatgtcaag atgctacctg actcggtgaa ggcgcgtcat atactgatta gccctaaggc
      241 tcaaaatcag gaggcagttg ctaaagccaa ggcaacagca gatagcctgc tgaatgtaat
      301 taaacgtgga gggagctggg atgatcttgc ctcacatttt tccgatgacc caggatctaa
      361 ggataagggt ggcgacctag ggtggtttcc cggcggtgtc atggtgaaaa attttgatga
      421 ggctgcattc aacctttcaa aaaatgaggt taaggtagtt gaaaccaatt acggatttca
      481 tattcttcag gtaaccgata gggggaagga atccaggaag gtgcagctgg ctactctgga
      541 gcgaaaagta gtaccaagta gcaccaccag ccagaggata tacagcgaag cttcgcaatt
      601 tgccttatct aacagaacct acgagcagtt taaaaccgct gctgatgcca aaaaacttac
      661 ccgacgggtt gcctccaacc tgctggccaa cgaccaaaat attgccgggc tggaatcacc
      721 ccgtgaagtt attcgctggg catttaaggc tgaagagggc gatgtttcaa acgtgtttga
      781 gttggccaac atgtatgtgg ttgcctctct taccgctgcc cgtagtgagg gagttgcacc
      841 cttaaagcag gttgttgccg atgtaagaac caaggtaatc agggataaga aggccgaaaa
      901 gttggcagca cgtgtgagtg atgctttaaa gggcgccagc gatatttttt ccgttgcggg
      961 taagcttaac ggcaaggtag agcacgccga aaatgttaca tttagctcgt ttactttgcc
     1021 ctatgcaggt tttgagccag ctgttattgg agtggcttcg gttagctccg agggtaagct
     1081 tcagggacct gtcaatggta acgccggggt ttacgttttg gctgttacat ctatagttac
     1141 cgatcaggga gatgccggaa tggaaaagtt aagattggcc aacacctacc agtccagggc
     1201 atactacgag gcttttgagg cgttgaaatc aaagattggc attgttgata agcgctacaa
     1261 cttctactag acagcatcga acctgaaagt tgttcactac tgaggggaaa taaaatttcc
     1321 ccttttttgc atcactgttt tttcgaacta aaagtttgca aaatggttat aattgaacag
     1381 gcaaaacgaa aattatcgga attattaacc aaataaaatg aaatgttatg aaggttgctg
     1441 taccaacaag aaacggtgtt gttgacgaac actttggcca ttgtgaggct tacaccgtat
     1501 tcacgattga ctcgggtaat aagattgaaa actcagaaat tcttccatcg cctcaggggt
     1561 gcggatgcca tagcaatatt gctgccactc tgaaggaaaa aggtgtagaa gttatgctcg
     1621 cagggaacat gggcgatggt gcattgaaca tgttagctgg tcatggaata caggtatttc
     1681 gtggctgttc gggcgatgct cgccggttgg ttgaaaattt cctgagcgga aatgttgagg
     1741 attcaggcga ggggtgtcat catcaccacg aacatgaaga gggtcataca tgcaatcatt
     1801 agcaagtata ttggctattc ggagctacat taagtgaggc ttttttaccc tgggctatga
     1861 ataatcgttg agaaacatta ccagtggtaa ttttttgtgg cgataaatcc ggttctgcac
     1921 cgaaaccgcc tttataattt tatctgcctt cactcccttt cagggttagt agtagatgtg
     1981 ccattttcct atgtcttttc ttgtgctgaa ccttttcgcc agccattgcc tgttttcagg
     2041 taggggatgc ttgtaggaaa ccagaactga cggcctttcc atcaggcctt taagccgatt
     2101 ggtatcaatg ctgtcagtga ggtcgtaaaa gtagcttttc catgccgaat tttcctcaaa
     2161 ctgcacctcc cgggcaagag tgttcgaacc atcatatagc gaaatcaccg gctttcgcat
     2221 gtcgaaagca atggagggtt tcatggtgtt gtagatgagt atattcctgt cctgtagatt
     2281 tttgctttta ataaagttgg agattggcct ggaaatgttg aattccaact catttttttg
     2341 catcagcagg ccgcttgtta taagaagaaa cccggaaacc aaaaacgaga cgagcacagg
     2401 tttgaatttt ctacccccct gtaaataacg ataaattgcc aaaatgacta caattagtag
     2461 aatgctggca atgcctactc cttttggtat aacgtaattg gtgtgtataa acatggttgc
     2521 cgttgcaact agtaggacaa tcattgagaa tatcgttacg cttcggtcga taaccttctc
     2581 ttttgagcta attagatttg ccatcaggtt ggcggtgagc attgccaaaa tgctgtagaa
     2641 cggcagtatg tacagaattc tttttgaggt agatattgaa aagaacacca gtggaattac
     2701 gaagcttagc gtaagcacag aatttatacc cttcttcagg atgctttttc tgttctgcac
     2761 cattaagtag ggtagaatga gtagccatgg aagacctaca agcggtgcaa aagctacgaa
     2821 gtaccagaat ggctccgaac ggttgaaaac atcctttcca aaccgttcca ccgtttgtct
     2881 gcctaaaaag tagtcggcaa acgctccgtt ctctctaatc aggaaaacaa accaccatag
     2941 cgaaataccc agaaaaacca ccagggaaag tagatggtga actccaaacc gatttttact
     3001 tttttcaatg cggttgtagg ctataataaa taccaatggt accagaagta ccaccggacc
     3061 tttagtacag aatcccaaag ccaggctaat tgcagttaag tagaggtacc gaaatttgcc
     3121 gtattttcga tacttaaccc aggagtatat tgacaacaga acgaaagttg ctaaaaacga
     3181 atcggttgtt agatttcgtg tagatatgag agttaatggg aagctgaagt atattatagc
     3241 tgcccataat gcggtaccac ggttgccaaa gagaatgttg gatagggcat acactaaaag
     3301 cagctgaata aggagggaga gctgcaggaa aaaacgggcg ccaagctcgt ttacgccaaa
     3361 aatttggtaa cctaaagcgg taagctggta ggttaacggg ggcttatggt agtgttgaat
     3421 attcaaaagg gtgggatgaa tgtagtcgcc cgactctagc atctcacgcg aaatttcagc
     3481 gtagcgggca tcgctacttt caacaacccc gtaggtacca acgttgatga acagaagcaa
     3541 ggtgcataga acaataaaaa tcgaaaatag caggtacttt tcatgcttta actttttaac
     3601 aaaccgcagg aacgtatatt taatcattga tagcaacgtt aatccggaat cagtgcgtcc
     3661 agactttgcc tagaaactaa cttcaaaata tcataacact atgcttatgg tgaaggttca
     3721 cccatgttat ttttataagt gtaaaggagc attacaattt tctattgata cactgtagga
     3781 cagttaactt tgctgccagc tggacagaat ggcatggatt attactgtgt taataggtaa
     3841 tttaagggct atttgttaat attttgatat tttacaccta gaagtaagca atctttttta
     3901 attccgtttg ctgtaggtaa tggttttgag tgggcagtag gggttgagaa ctcattatgg
     3961 gtttagtaga aaaaatgcta attttgaaat taaaatagc
//
LOCUS       DAOOQX010000099         3983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_26977_length_3983_cov_2.926935, whole genome shotgun sequence.
ACCESSION   DAOOQX010000099 DAOOQX010000000
VERSION     DAOOQX010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26977_length_3983_cov_2.926935"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="PLX53_02445"
     CDS             <1..1274
                     /locus_tag="PLX53_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOY71969.1"
                     /translation="AIAQIQDDMMRQVEHFRSIGHMVEAKRIQQRVEYDLEMIKELGY
                     CPGIENYSRYFDGRQAGMRPFCLLDYFPNDFLTIIDESHVTLSQVRAMYGGDNSRKQT
                     LVEYGFRLPAAIDNRPLRFDEFEGLIGQTIFVSATPSDYELRLCQGVVVDQVVRPTGL
                     LDPPIIVRPSLNQIDDLIGEINASAKRDERVLVTTLTKRMAEELVKYLVKFDIRCRYI
                     HSDVDTLERVQIMEDLRKGLFDVLVGVNLLREGLDLPEVSLVAILDADKEGFLRSARS
                     LTQTAGRAARNLNGRVIMYADKITGSMQLTMDETNRRREKQLKYNELHGITPTQIVKP
                     KKSIMAGKLSTGEVVKPYVEPDTVDFAADPVVQYMTKPELAEAIERARKEMEREAKDL
                     NFFEAARYRDEMFALQRLFETKFGAKKSKST"
     gene            1311..2267
                     /locus_tag="PLX53_02450"
     CDS             1311..2267
                     /locus_tag="PLX53_02450"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOY71970.1"
                     /translation="MSTKKDFLLVIPFLMISNLITAQISETVVKELKTQSEDSILGWK
                     KGFVTGINFSQTSLTNWAAGGQNSLSLATMLSGFVSFKNEKVAWDNTLDIGYGFLNQD
                     GVKYNKKTDDRVDLSTKYGYRASKNIYYALLLNFKTQMAKGYDYPNDSTRNMISNFFA
                     PAYLVGALGMDIKPSSYLSLFVAPFTGKFTFVTDSILSSTGAFGVKPGKEYRSEFGGY
                     VRISFSKADFKPEVLKNLSFTTKVDFFSNYADKPQNIVVNWEALVAIKVNKYLNMNLS
                     TMLIYDDKIKINIDKNGDGTFDSKSPRVQFKEILGVGLSVKL"
     gene            2655..3503
                     /locus_tag="PLX53_02455"
     CDS             2655..3503
                     /locus_tag="PLX53_02455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOY71971.1"
                     /translation="MKRAITLSIFAIAIAALISCTGNKSNKAGETSTPPTMQPVVKGT
                     EITYYDGSTPLKGYIAYDESLTGKRPGILVVHEWWGNNDYSRMRANMLAELGYVAMAV
                     DMYGNGLILDNPKDAQNTANQFYTNPALMKERMLVAYNTLIADSLVNAERIAAIGYCF
                     GGAVVLNSASMGVPLDAVVSIHGGLTGFKATPEMKNVNVLICHGGADPFVPQADIDNF
                     KSEMQNMGVNYEFKVYEGATHAFSNKASTEAGKKFNMPISYNAAADSASWSDMVTFFN
                     KYFPAK"
     gene            3525..>3983
                     /locus_tag="PLX53_02460"
     CDS             3525..>3983
                     /locus_tag="PLX53_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOY71972.1"
                     /translation="MQVEFFHDVLCAWCFALSPRMRRLVAEFPEIEVIHRSFALASDA
                     DRLVEMFGSKEAAKREILNHWRNANENDDEHRIHTEDMALRTFDYPYSMPGLLACKSA
                     ELQGGQLAHWNMFDRIQKAHLVETQNIADFNVLLQCADEVGLDTERFKNDF"
BASE COUNT         1141 a          927 c          939 g          976 t
ORIGIN      
        1 aggccattgc ccaaattcag gacgatatga tgaggcaggt ggagcacttc agaagcatag
       61 gccacatggt tgaggccaaa aggattcagc aacgggtgga gtacgacctg gaaatgataa
      121 aggagttggg ctactgccct ggtatagaaa actactcccg ctacttcgat ggccggcagg
      181 ccgggatgag gcctttctgt cttctcgact actttccaaa tgatttcctg acaataattg
      241 acgaaagcca cgtaaccctt tcgcaggtca gggcgatgta tggaggtgat aactcgcgca
      301 agcaaacgct cgtggagtat ggctttaggc tacctgccgc tattgacaac cgtccacttc
      361 gattcgacga attcgagggc ttaatcgggc aaaccatttt tgtaagcgca acaccatcag
      421 actatgagct tcggctgtgc cagggggtgg tggttgacca ggtggtcagg ccaaccggcc
      481 tgctggatcc acctattatt gtcaggccta gccttaacca aattgacgac ttgattggtg
      541 aaataaatgc cagtgcaaaa cgcgacgaaa gggttttggt aaccaccctc accaagcgta
      601 tggctgaaga gctggtaaag taccttgtga agttcgacat tcgttgccgg tatattcatt
      661 ccgatgtgga taccctggaa cgggttcaaa taatggagga tttgcgaaaa gggctatttg
      721 acgtgctggt aggcgtaaac ctacttcggg agggactcga cctgcccgag gtttcgctgg
      781 tagccatact ggatgccgat aaggagggtt tcctgcgctc agcacgctcg cttactcaaa
      841 ccgcaggtcg ggctgcccgt aaccttaacg gacgggtaat catgtatgcc gataaaatca
      901 caggctccat gcagcttacc atggatgaga ccaacaggcg tcgcgaaaag cagctgaaat
      961 acaacgagct gcatggaatt accccgactc aaattgtcaa gcccaaaaaa tcaatcatgg
     1021 ccggaaaact ttcaaccggc gaggtggtta agccctacgt tgagcccgac acggtggact
     1081 ttgccgccga tcccgttgtt cagtacatga caaaacccga attggctgaa gccattgaac
     1141 gcgcaagaaa ggaaatggaa agggaagcca aagacctcaa cttttttgaa gccgcccgat
     1201 accgcgatga aatgtttgcc ctgcaaaggc tattcgaaac aaaatttgga gcaaaaaaat
     1261 ccaaatcaac ctaaaaaatc acccacaatg tcattgtaat aaccgaaacc atgagcacta
     1321 aaaaagattt tctgcttgtc atcccgtttc taatgattag caacctaatt acagcacaaa
     1381 tttccgaaac cgtagtaaag gagttgaaaa ctcaaagtga ggattcaatc cttggctgga
     1441 agaagggatt tgtaaccgga atcaacttct cacaaacctc gctgacaaac tgggcggcag
     1501 gaggacaaaa ttccctatcg cttgccacca tgcttagcgg gttcgtcagc tttaagaatg
     1561 aaaaggtggc atgggataac accctcgata taggctatgg gttcctcaac caggatgggg
     1621 taaagtacaa taaaaaaacc gacgatagag ttgacctttc caccaagtac gggtatagag
     1681 cctcaaaaaa tatttactac gccctgctgc tgaactttaa aacccaaatg gcgaaagggt
     1741 atgactaccc caacgatagc acccgaaaca tgatttcgaa cttttttgcc cccgcatacc
     1801 tggtgggtgc gcttggcatg gatataaaac ccagcagcta cctaagcctt ttcgttgctc
     1861 cgtttacagg aaaatttaca tttgttaccg attccatcct gtcgagcact ggtgcctttg
     1921 gggtaaaacc gggcaaggag tatagatcgg aatttggtgg ctacgtgcgg ataagcttct
     1981 caaaggctga ctttaagcct gaggttttaa aaaatctatc gttcaccacc aaggttgatt
     2041 ttttctcaaa ctatgccgac aaaccccaaa acatagtggt aaactgggaa gcgctggttg
     2101 caattaaggt gaacaagtac ctcaacatga acctgagcac catgctcatc tacgacgaca
     2161 agattaaaat taacattgat aaaaacggag atggtacctt cgactcaaag tccccacgag
     2221 ttcaattcaa agaaatttta ggagttggtc tatctgttaa gctataggtt ttcgggctaa
     2281 tacaaacatt ttcctgtagt cataatttta gcagggttgc aggtggttcg tcccgacgtg
     2341 caaggtgctt tgcagatcct catcattggt gcatattccc tgagaagtac gaaaaaatct
     2401 aaccctggct aaaacttctc taaatcacat ttttgtcagc tagccttcca taacttttgg
     2461 tgaggcggcc gttcgtgcat gcttggcctg atattctcct gaatttcaat aacctgaaag
     2521 agctgcgttt gcaaaaattc gcctgatgac taaaaaatac cacctaattc cagctattgt
     2581 aactccctgc cataagcata gttgaacttt ttgactgtac ccgttgttat atgtttgaaa
     2641 aaaataatta taccatgaag agagcaatta ccttatcaat ttttgcaata gcaattgcag
     2701 cgctgatttc ctgcaccggg aataaaagta acaaagcagg tgaaacttct actcctccaa
     2761 ccatgcaacc tgtagtaaag ggaacagaga ttacctacta cgacggctca accccactta
     2821 agggctacat tgcctacgac gaaagtttaa ccggaaagcg tccaggcata ctggtggtcc
     2881 acgagtggtg gggcaacaac gattactcca gaatgcgcgc caacatgctg gccgagcttg
     2941 gttatgtagc catggcagtt gacatgtacg gcaacgggtt gatactcgac aaccccaagg
     3001 atgcccaaaa cactgccaat cagttttaca caaatccagc cttgatgaag gagcgcatgt
     3061 tggtggccta caacacacta attgccgaca gcctggttaa tgccgagcgt attgctgcaa
     3121 taggctactg ttttggtggt gctgttgtgc ttaactcagc tagcatgggt gttcccctgg
     3181 atgcagtggt aagcatacat ggtggattaa ctggatttaa agctaccccc gagatgaaga
     3241 acgtcaacgt gctgatttgc catggcggcg ccgatccctt tgttccacag gctgatattg
     3301 acaactttaa gagtgaaatg cagaatatgg gggttaacta cgagtttaag gtttacgagg
     3361 gtgccaccca tgccttctcc aataaagcct caaccgaagc cggcaaaaag ttcaacatgc
     3421 ccatttccta caatgcagcc gccgattcgg cttcgtggag cgatatggta acattcttca
     3481 ataagtactt ccccgcaaaa taacttacat gaaaagttgc cactatgcag gttgaatttt
     3541 ttcatgatgt tctttgtgcc tggtgctttg cactttcccc acgtatgcgg aggctggtgg
     3601 cagaattccc cgaaatcgag gttatacatc gctcttttgc ccttgcatca gatgctgata
     3661 ggctggtaga aatgtttggc agtaaggaag ctgccaagag ggaaattctc aaccactggc
     3721 gaaatgccaa cgagaatgat gatgagcata gaatacacac cgaggacatg gcgctaagaa
     3781 catttgacta cccctactcc atgccaggcc tgctcgcctg caagtcagcc gagttgcagg
     3841 gtgggcaatt ggcgcattgg aatatgttcg acaggataca aaaggctcac ctggttgaaa
     3901 cacaaaacat agccgatttt aatgtgctgc ttcaatgtgc tgacgaagtg gggcttgata
     3961 cggaacgttt taagaacgac ttt
//
LOCUS       DAOOQX010000100         3967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_27122_length_3967_cov_4.477249, whole genome shotgun sequence.
ACCESSION   DAOOQX010000100 DAOOQX010000000
VERSION     DAOOQX010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27122_length_3967_cov_4.477249"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2031
                     /gene="polA"
                     /locus_tag="PLX53_02465"
     CDS             <1..2031
                     /gene="polA"
                     /locus_tag="PLX53_02465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOY71973.1"
                     /translation="IVTDLDLKVDIDKDLVSREPNTNELLKLFQELEFRSLIREFDRQ
                     PVAAKKEYIQGSLFGEDPEQKVTVIQNLTTIKDIKADYTAVTTAEERKLVVEEISGCT
                     EFAFDTETTGLDPISDSLVGLSVATKPLKAWWIPVPDDRESVSKILAEFKPLFENPQV
                     AKIGQNIKFDMQMLANYGISVKGELYDTMLAHYLLDPDSRHNLNHLSEIYLGYKPIEI
                     EELIGKKGSKQANMRNVPLEKITPYACEDADIALQLKLRLFAELESQGLTKLYFLLEA
                     PLVEVLAQMERNGVCLDVQSLQEYGKILNSELLKLDAEIKAMAGVPDLNISSPKQLGE
                     VLFDKLRIATDVKMTKTKQYSTSEEELLKYQSAHPIVGKILEFRSIKKLLSTYIETLP
                     GLVNTKTGRIHTSFNQAVAATGRLSSNNPNLQNIPIREENGREIRRAFIPSPGSYSML
                     SADYSQIELRLMAHMSGDSAMMEAFRNGEDIHAATAAKIFKVPLEQVSREQRSRAKTA
                     NFGMIYGISAFGLSQRLGISRTEAKELIDEYFRTYSGVKRYMDSCIAQARERGWVETL
                     FGRKRYLNDIKSSNQTVRGMAERNAINAPIQGSAADIIKLAMIGIHSEFKRLKLQSKM
                     IIQVHDELVFDVYQPELEQVKEIARSQMEGAAALQIPLVVDIGVGKNWLEAH"
     gene            complement(2060..3088)
                     /gene="dusB"
                     /locus_tag="PLX53_02470"
     CDS             complement(2060..3088)
                     /gene="dusB"
                     /locus_tag="PLX53_02470"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOY71974.1"
                     /translation="MRIGEVELGEKPLFLAPMEDVTDPSFRYVCKQFGADVMYTEFIN
                     ADGLIRDAGSSVAKLNIYDYERPIGIQLYGHIIESMVEAARIATACRPDIIDINFGCP
                     VKKIANRGAGAGMLRNINAMVEMTKRIVEATSIPVTVKTRLGWDDESKNIVEIAERLQ
                     DVGIKAITIHGRTRAQLYKGKADWTLIGAVKANPRITIPVIGNGDIDGPVIAGEMFEK
                     HGVDGIMVGRASFGRPWIFREIKHYLSTGQLMEPLTASQKVEIAKLHFQKSLEVKGHK
                     TGILEMRRHFSAYFKGLPNFKEIRLKLLTSTEPEVIEGLLELIRSTYGDFVADGSINP
                     SPWADSAD"
     gene            3342..>3967
                     /locus_tag="PLX53_02475"
     CDS             3342..>3967
                     /locus_tag="PLX53_02475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOY71975.1"
                     /translation="MRAGILSNLSDAKKIVALIILVLLGSFLFTLLGYLVIMAVYGTD
                     SLGWLMNVTISNDTIGALKFLQIVQTIGMFVVPGFTAAWLFSSHPFGYLGFKPSTVQN
                     FITVAVMMVISIPAINLLASINELIPLSGWMVEMERNAEILTEAFLKTDSVGTFLLNL
                     LMIAILPAVGEEIIFRGILQKHLVSATRSRWLGILITAALFSAFHLQF"
BASE COUNT         1059 a          986 c          924 g          998 t
ORIGIN      
        1 attgttaccg atctcgacct aaaggttgat attgacaagg acttggtgag cagggaaccc
       61 aacaccaacg aactcctgaa gctatttcag gaactggaat tcaggtcgct aatcagggaa
      121 ttcgaccggc aaccggtagc cgcaaaaaag gagtatattc agggtagtct gtttggggaa
      181 gatccagagc aaaaggttac cgtaatccaa aaccttacaa ccataaaaga tataaaggca
      241 gattacacag ccgttacaac agcagaggag aggaagcttg tggtagagga aatttcgggg
      301 tgtaccgaat ttgccttcga caccgaaaca accgggttag atcctatctc cgatagcctg
      361 gttggcctgt cggttgccac aaaacccctg aaggcatggt ggatacccgt tcctgacgat
      421 cgtgagtcgg ttagcaaaat tttagccgag ttcaaacccc tgtttgaaaa tccccaagtg
      481 gcaaaaattg ggcagaatat aaaatttgac atgcaaatgc tggccaacta tggaatttcc
      541 gtcaagggag aactctacga caccatgctg gcccactacc tgctcgatcc tgactcgcgg
      601 cataacctga accacctgtc ggaaatctac ctcggttaca agcctattga aattgaggag
      661 cttatcggga aaaaaggctc aaagcaggca aacatgcgaa acgtccccct ggagaaaatc
      721 accccatacg cctgcgagga tgccgacatt gccctacagc tgaaattgag gcttttcgcc
      781 gaacttgagt cgcagggtct tacgaaactc tacttcctac tcgaggcacc attggttgaa
      841 gtgcttgctc aaatggagcg caatggtgtt tgcctagacg tgcaaagcct acaggagtat
      901 ggaaagattc ttaactccga attgcttaag ctggatgccg aaattaaggc catggcgggt
      961 gtccccgacc tcaacatctc ctcgcccaaa cagctaggcg aggtgctttt cgacaaactt
     1021 cggattgcta ctgatgtgaa aatgaccaag accaagcagt actccaccag cgaggaggag
     1081 ctgctaaagt accagagcgc ccatccaatt gtcggaaaaa tactggagtt tcgctccatc
     1141 aaaaagctcc tgtccactta catcgaaacg cttcccgggt tggtgaacac gaaaaccggg
     1201 agaattcata cctcgttcaa ccaggcagtt gccgcaaccg gacgcctaag ctccaacaac
     1261 cccaacctgc aaaacatacc catacgcgag gagaatggcc gtgagatacg gagagccttc
     1321 atcccatcac cgggcagcta ttccatgctc tcggccgact attcccaaat tgagcttagg
     1381 cttatggccc acatgagcgg cgattcggcc atgatggagg cttttcgaaa cggcgaggat
     1441 attcatgccg caacggctgc caaaatattc aaggttcccc ttgaacaggt gagccgtgag
     1501 caacggagcc gggcaaaaac agccaacttc ggaatgattt acggcatctc ggcctttggt
     1561 ctctcccagc ggctcggcat ctcgcgcacc gaagcaaagg agctgattga cgagtacttt
     1621 cgaacctact ccggggttaa gcgctacatg gacagctgca ttgcccaggc acgcgaacgg
     1681 ggctgggttg aaaccctttt tggccgcaag cgctatctaa acgacattaa atcgagcaac
     1741 cagacagttc gcggaatggc cgaacgtaac gctattaacg ccccaataca gggttctgca
     1801 gccgatataa tcaagctggc catgattggc attcactccg agtttaaacg gttgaagctg
     1861 cagtcgaaaa tgattataca ggttcacgac gaactggtgt ttgacgttta ccaaccggag
     1921 ctggagcagg ttaaggaaat tgccaggtcg caaatggagg gtgctgccgc cttacaaatt
     1981 cccctggtgg tggatattgg ggtaggcaaa aattggctgg aggcccacta gctgcatttc
     2041 tcgaaacgga gaaaaatgtc taatcggccg aatctgccca gggtgagggg tttatactgc
     2101 catctgccac aaaatcacca taagttgacc tgatcagttc aagtaagcct tctatcacct
     2161 ctggctcggt agaggtaagc agctttagcc tgatttcttt aaaattggga agtcccttga
     2221 agtaggctga aaaatgacgc cgcatttcga ggattccggt tttatgacct tttacctcga
     2281 gcgatttttg aaaatgcagc ttggcaattt ccactttttg gctggctgta aggggctcca
     2341 tgagttggcc ggtagaaagg tagtgcttaa tctccctgaa tatccatggc cttccaaatg
     2401 atgccctgcc aaccatgatg ccatctacac cgtgtttttc gaacatctca ccggctatca
     2461 ccggaccgtc aatatcgccg ttacctatca ccggaatggt tattcggggg ttggctttca
     2521 ccgcaccaat aagagtccaa tcggctttgc ctttgtagag ctgagctcgg gtgcgcccat
     2581 gaatggttat agccttgatt ccaacatcct gcagccgctc ggcaatctca acaatgtttt
     2641 ttgattcgtc gtcccaccct agacgggtct tcacggtaac tggaatgcta gtggcctcca
     2701 caattcgttt ggtcatctca accatggcat tgatattcct aagcattccg gctccagcac
     2761 cccgattggc tattttttta accggacagc caaagttaat gtcgataata tcgggacgac
     2821 aggctgtagc aatccttgcg gcctctacca tactttcaat aatatgaccg taaagctgga
     2881 tgcctattgg tctttcgtag tcgtaaatat tcaactttgc cacgctgctg ccggcgtcac
     2941 gtataagccc atcggcatta ataaactcgg tgtacatcac atcagcgccg aactgcttgc
     3001 atacataccg aaacgacgga tcggtaacat cctccattgg tgctaaaaaa agaggctttt
     3061 cgcctagctc tacttcgcca atacgcattt gtcatgcagt tgagctgcaa aagtaaaaaa
     3121 tgtttttccc tctgcataac caacgagccg aaagcgctgg tctgcaaatg agtaaaaata
     3181 gattttatcg gcttctgatt atctggcatt ttgacaagta aatccacagt ttaccgaaaa
     3241 cttctacctt tgcagctgca ctttaaccag ttgggttttg cttgagtttt ttttcgctat
     3301 ggtatccatt ttttccctgc actaattcta acaaccatcg tatgagagcc ggaattctat
     3361 cgaatctttc tgatgctaaa aaaattgttg cgctgattat ccttgttcta ttagggtcgt
     3421 ttctttttac cctactaggc tacctggtta taatggcagt gtatggaacc gattcgcttg
     3481 gttggctaat gaacgtaaca atatcgaacg atacaattgg tgcgttaaag tttttgcaaa
     3541 ttgtacaaac aataggcatg ttcgttgtgc ccggattcac agcagcatgg ctattttcca
     3601 gtcatccatt tggctacctg ggttttaaac catccaccgt tcagaatttt ataactgtag
     3661 ccgtaatgat ggttatctca ataccggcca tcaacctgct ggcctccatt aacgagctga
     3721 tacccctatc gggctggatg gtagaaatgg agagaaacgc cgagattcta actgaggcat
     3781 ttctgaaaac cgacagcgtt ggaacgtttc tgctaaacct gctgatgatt gccattcttc
     3841 ctgccgttgg ggaggagata atttttcgag gaattttgca aaaacacctg gtatcagcta
     3901 cccgcagcag gtggctcggg atactgataa cagcagcgct attcagcgca ttccacctcc
     3961 aatttag
//
LOCUS       DAOOQX010000101         3956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_27224_length_3956_cov_3.183286, whole genome shotgun sequence.
ACCESSION   DAOOQX010000101 DAOOQX010000000
VERSION     DAOOQX010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27224_length_3956_cov_3.183286"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..774)
                     /gene="radC"
                     /locus_tag="PLX53_02480"
     CDS             complement(76..774)
                     /gene="radC"
                     /locus_tag="PLX53_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOY71976.1"
                     /translation="MEQEYKRLTIKDWAVEDRPREKMLHQGIGSLSDTELIAILLRSG
                     NPEETAVELAQRILNMAQNNLNELGKFTIHQLTQVKGVGEAKAITLLAALELGRRRKS
                     VEAINREQITSSRNVMEIFQPLLADLPHEEFWVLLLSRANKVLDRVRVSQGGVTGTVT
                     DVKLIIRAAVEKLASAIIVVHNHPSGNPKPSEKDIAITEKLKQAVDFFDIALLDHLII
                     TDSKCYSFADEGTL"
     gene            complement(1054..2037)
                     /locus_tag="PLX53_02485"
     CDS             complement(1054..2037)
                     /locus_tag="PLX53_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOY71977.1"
                     /translation="MIPSSSIFSLKPDGDFEKIALEVFRFQSQHVRVYHDYIQQLGVN
                     PDAVASTDEIPFLPIQLFKTQDVIAEACQPEATFTSSGTTGNEVSRHRVADLSLYRES
                     FTRTFSIFYGSPSDYCILALLPSYLERSGSSLVFMVDELIRQTGNPDSGFYLNQLYAL
                     TEKLSALDRSGQKTILIGVSFALLDLAERFTFNLQNTIIMETGGMKGRRKEVVREELH
                     STLCSAFGVNVIHSEYGMTELLSQAYSTGKGMFRCPPWMRVVIGSTDDPFCLVGDGRT
                     GIIKIIDLANLYSCSFIQTQDMGKLHSDGCFEVLGRMDDAPIRGCNLLVYD"
     gene            complement(2147..3409)
                     /locus_tag="PLX53_02490"
     CDS             complement(2147..3409)
                     /locus_tag="PLX53_02490"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOY71978.1"
                     /translation="MITIDSYSFKGKKAIVRVDFNVPLDKKTLEVTDDTRIRGAVPTI
                     KKILGDGGAVILMSHLGRPDGTPQDKYSLRHVVPAIEKNLGQKIKFAADCMGEETKKM
                     AAGLKPGEVLLLENLRFYEEEEGKPYSLPETASEEEKKEAKARVKESQKNFVKQLASY
                     ADCYVNDAFGTAHRAHASTALIAAHFPGNSMFGYLIESELKAMDKVLKSPTKPFTAIM
                     GGAKVSDKILIIENLLDRVDNLIIGGGMTYTFICAQGGKVGKSICETDKLELANQLLE
                     KAKAKGVKVYLPVDAVNADAFDANANTQITPIDSTPDGWMGLDIGPKTIAKFREVILE
                     SKTILWNGPMGVFEMDKFAAGTSEIAKAIAEATQRGAFSLVGGGDSVAAVNKNHLADK
                     ISFVSTGGGAMLEYMEGKELPGIKAIRG"
BASE COUNT          952 a          917 c          947 g         1140 t
ORIGIN      
        1 tggttaaaca tggtataagt tgttagctat tagtattttc tgcatgtcag gttttatggc
       61 tattcaagtg gttgattaca gagttccctc gtctgcaaat gagtagcatt tgctatcggt
      121 aatgataagg tgatcgagta gcgcaatatc gaaaaaatct acagcctgct tgagcttttc
      181 cgtaatggca atatcctttt cgctaggttt ggggtttccc gacgggtggt tatgaaccac
      241 aataatagca gaggccaact tttcaacggc tgcccttatg attagtttta catcggtaac
      301 cgtgccggtt accccaccct ggctaaccct aaccctatcc agcaccttgt ttgcccggct
      361 caggagcagc acccagaact cctcgtgggg caggtcggcc agtaagggct gaaaaatttc
      421 catcacattc cggctagatg taatctgctc gcggtttatg gcctccacag actttcgcct
      481 acggcccaac tccaacgctg ccaaaagggt tatggctttg gcttcgccta ctcctttaac
      541 ctgtgtaagc tgatggatgg taaacttacc cagctcgttg aggttattct gggccatatt
      601 taatatccgt tgggcaagct caactgctgt ttcttcaggg tttccggaac gcagcaggat
      661 ggcaatcagc tccgtatcac ttaggcttcc aattccctgg tgaagcattt tctcccgggg
      721 ccggtcctcc acagcccaat cctttatggt tagcctttta tactcctgct ccattggttt
      781 gcctttttca actgggtatg aacggcaaga aacagcttct gtatcaagaa ttagttttag
      841 acatcaaacc tccaaaaaag gctaatttag ctcctttatt gcatagttca tacaggtgaa
      901 tgcaactttt taacagggaa aaagggttgc atgccatgct acatgcaatg gtgtggcctg
      961 atgttgggga tgtgtaaaat ttaaaacaag cgtagaaagt agatgctatg ggttgtgcac
     1021 ataggttcac tttggcaatg cttccaggtc tattcaatcg taaaccagta agttgcaacc
     1081 ccttatgggt gcatcatcca tacgaccaag cacctcgaaa caaccatcgg aatggagctt
     1141 tcccatatcc tgggtttgaa tgaatgagca ggaataaagg ttggccaggt caataatttt
     1201 gataattcct gttctcccat cccctacaag gcaaaagggg tcatcggtgc tacctataac
     1261 cacccgcatc cagggtggac accgaaacat ccctttaccg gtagaatatg cctgtgaaag
     1321 caattccgtc atgccgtact cggagtgaat gacgtttacc ccaaatgcgc tgcaaagcgt
     1381 tgagtgcagc tcctccctga caacctcctt acgacgcccc ttcatacctc cggtttccat
     1441 gatgatggta ttctgaaggt tgaaggtaaa cctttcggcc aggtcgagca gagcaaacga
     1501 gaccccgata aggattgtct tttgaccgga gcggtcaagg gcgctgagtt tttccgttag
     1561 ggcatagagc tggttgaggt agaaaccgct gtcaggattg ccggtttgcc tgataagctc
     1621 atcaaccatg aacaccagcg atgagcccga acgttcaagg tatgagggca gaagtgcaag
     1681 aatgcagtag tccgagggag aaccgtaaaa tattgaaaac gtgcgggtga agctttcacg
     1741 gtagagcgat aaatcggcca cccgatggcg gctaacctcg ttacccgtag ttccactgct
     1801 ggtgaaggtt gcctccggtt ggcatgcttc ggctataaca tcctgcgttt tgaagagttg
     1861 aatggggaga aacggaattt cgtcagtgga agcaactgca tcggggttta cgccaagctg
     1921 ttgaatgtag tcgtggtaaa cccttacatg ctgggactga aacctgaaaa cctccagggc
     1981 tattttttca aaatcaccat ccggtttaag gctgaatatg ctggagctgg gaatcatttg
     2041 attttgcctt tacctgcttt tacgggttat gagtagctga aaatacaaag tcagggcttt
     2101 ttttaaaacc ctgacggtat gcttaaatgg cgtgattaga gggttgttat cctctaattg
     2161 ccttaattcc gggcagttct tttccttcca tgtactccag catggcgccc ccaccggtgg
     2221 agacgaagga aattttatcg gccaggtggt tcttgttgac agcagctacg gagtcgcccc
     2281 cgccaaccag gctaaacgct cccctttggg ttgcttcggc aatggccttg gctatttctg
     2341 atgttccggc agcaaatttg tccatttcaa aaacgcccat ggggccgttc cagaggatgg
     2401 tttttgattc gaggataacc tcacggaact tagctatggt tttggggcca atatccagac
     2461 ccatccagcc gtcgggagtg ctgtcgatag gggtaatctg ggtgttggca tttgcatcaa
     2521 atgcatcggc attcactgcg tcaacaggca ggtaaacctt tactcctttg gcctttgctt
     2581 tttcgagcag ttggttagcc agctcgagct tatcggtttc gcagatagac ttaccaactt
     2641 ttcctccctg ggcgcagatg aaggtgtaag tcataccgcc accaattatc aggttatcaa
     2701 ccctatcgag caggttttcg attatcagga ttttgtcgga caccttggcg cctcccatga
     2761 tggctgtaaa gggcttggtg ggcgacttca gcaccttgtc cattgccttg agttcgcttt
     2821 caataaggta gccaaacatg ctgttgccgg gaaagtgggc tgcaataagc gccgttgaag
     2881 cgtgtgcccg gtgggcagtt ccaaaagcat cgttaacgta gcagtcggca tagctggcca
     2941 gctgcttaac aaaattcttc tggctctcct taacccttgc cttggcttct ttcttttcct
     3001 cctcgctggc ggtttcggga aggctgtaag gtttaccttc ttcttcctcg tagaatctaa
     3061 gattttcgag cagcaacacc tcgccgggtt ttaggccagc tgccattttt ttggtttcct
     3121 cgcccatgca gtcggcggca aattttatct tctgccccag atttttttca atagccggta
     3181 ccacgtggcg caacgagtac ttatcctgtg gtgtgccgtc gggccggcca aggtgcgaca
     3241 tgaggatgac agctcctcca tcacccaaaa ttttttttat tgttggaacg gcacccctta
     3301 tccgggtgtc atcggttacc tcaagggttt tcttgtcgag ggggacgtta aagtcaaccc
     3361 ttacaatggc ttttttccct ttaaagctat agctgtcaat ggttatcatg gcttaacggg
     3421 tatttgattt caaagcaaag ttagcatagc gttgcaaaaa ataggctatt tcaagcgaat
     3481 atatttacgt aatcgtttga aaaatatcat tttaaggtat gttttaatag gaaaggctac
     3541 ttcttacagc agtagtgaga tttcctgaat ttctaagtaa attttcagga gcatgaggtc
     3601 gtaaaaataa ataatcaact cagcagctcg tcgtctccgg ggtatgctgg ggctgaatcg
     3661 caaacgtgaa atgatttgaa gtcaagcccg ggtagtctgg cgtaaataat ttagaaaatt
     3721 ttcagctgca tgtggggtaa agctgccaat ggggatttgt tttatctgca ttaaatgtta
     3781 aagtcttcct ggttaatatg ctactttcct ctgtgttttg cttcatcaag ctaatgatgt
     3841 gtcaaagcag cttttcaaaa attatacttc caatcttttc ggaaggtggg gatagtgtcc
     3901 cctttctttc aaatcccctt ttctccaaaa gtgctgccag attgctgtcg tatagt
//
LOCUS       DAOOQX010000102         3947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_27317_length_3947_cov_3.178828, whole genome shotgun sequence.
ACCESSION   DAOOQX010000102 DAOOQX010000000
VERSION     DAOOQX010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27317_length_3947_cov_3.178828"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLX53_02495"
     CDS             <1..772
                     /locus_tag="PLX53_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOY71979.1"
                     /translation="TIRYRLSGQGPVVILVHGYLENLEIWEDIVPDLEKNYTVLRVDL
                     PGHGKSACNHQTISMDLMADSISAVMTTLDIKKAFMVGHSMGGYTTLAFAERYPNWLN
                     GFCLLHSSANADSSEKRQSRMNDIETVKNGGKRDLVYLSVPRLFANQNLEKMDDIVDK
                     FIKIGLSTPDQGIIGALNGMAKRPDRNYVIENANIPVFFIFGKHDNLISMEVAEALME
                     RHKKARTVILNNSGHMGFVEEKDQTTAAITSFLTQVYS"
     gene            complement(763..2436)
                     /locus_tag="PLX53_02500"
     CDS             complement(763..2436)
                     /locus_tag="PLX53_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOY71980.1"
                     /translation="MAYQNSKKDIYYPIVIALVLAAGILIGQRFNGKSQKPGTLVYNR
                     TDKLSSVISYIEEEYVDSISVSTLVENTIPAILKNLDPHSIYIPASELQSVSEPLEGG
                     FDGIGIQFNMSNDTAVVVNTIKGGPSERLGILAGDRIVKVNGKLIAGVKFPMDSIPIL
                     LKGPTGTKVKVEIKRKDVDELIDFEITRDKIPISSVDIAYMVTPEIGYIKITTFAKTT
                     YKEFLDAMAKLESQGMKKLILDLRSNGGGYMDAATKIANEFLKPNQLIVYTQGKSRTR
                     QDVYSTGTGKYQDIPLQVLIDEFSASASEIFAGAIQDNDRGQIVGRRSFGKGLVQEQI
                     FFSDGSALRLTIARYYTPSGRCIQKPYTPGDDNYFNDITSRYLHGEFEVADSIQFADS
                     LKYYTVGGRTVYGGGGIMPDIFVPLDTNGITPYLQQVSRRNLIYRFAFNFTDQHRKET
                     RGLKDFPSVDRFLNKFDLLSDFIKFAEQNGVPRNEKEISVSKKILDAQVKATIARNII
                     DNEGFYPYIRNIDETLLKAIGLMEQSVSQPTTSALHRSVNSRVLNQAQL"
     gene            complement(2518..2967)
                     /locus_tag="PLX53_02505"
     CDS             complement(2518..2967)
                     /locus_tag="PLX53_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOY71981.1"
                     /translation="MNKTTSFNDPKQLQFDRRYLEMAKIWAKNSYCKRRQVGALIVKN
                     RMIISDGYNGTPSGFENICEDENGLTKPYVLHAEANAITKVAKSGNSSEGATLYVTSS
                     PCMECAKLIIQAGIKRVVFCDEYHNPDGLSLLSRAGIDVVKLDIEEL"
     gene            complement(3075..3158)
                     /locus_tag="PLX53_02510"
     tRNA            complement(3075..3158)
                     /locus_tag="PLX53_02510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3122..3124),aa:Leu,seq:caa)
     gene            complement(3337..>3947)
                     /locus_tag="PLX53_02515"
     CDS             complement(3337..>3947)
                     /locus_tag="PLX53_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOY71982.1"
                     /translation="ILSSVFIDTLHMIYGYHPLVDDMLLSSIFGGVLIGFGLGLIFKS
                     KATSGGSDIIAMIIAKYTRLPLGQLMIYVDSTIVLIALVAFKDWKIPLYSWVVIYITG
                     KVIDMTIEGASYDKALFIISDRYEEVRSKIINDLERGGTLISVKGMYSGEEKKLIFTN
                     VSRREVFMLKDYIREIDPKAFITVIDANEILGEGFKPISEAL"
BASE COUNT         1071 a          867 c          815 g         1194 t
ORIGIN      
        1 caccattcgc tacaggttaa gcggacaagg cccggtggtt attcttgtcc acggctatct
       61 ggaaaacctt gagatttggg aagatattgt tcccgatttg gagaaaaact ataccgttct
      121 acgggtcgat ttgcctggtc acggaaagtc ggcttgtaat catcaaacca ttagtatgga
      181 tcttatggcc gatagtatta gcgctgttat gaccaccctc gacattaaaa aagcctttat
      241 ggttggacac tcaatgggtg gttataccac gcttgccttt gcagaacgat accctaactg
      301 gttaaacggc ttttgcttgc ttcactcatc ggctaacgca gactcgtcgg aaaagcgtca
      361 gagcaggatg aatgacattg aaaccgttaa aaatggtgga aagcgcgacc ttgtttacct
      421 aagtgttcct cgccttttcg ccaaccagaa tttagagaaa atggacgaca ttgttgataa
      481 attcatcaaa ataggactaa gtacacctga ccagggaata atcggggcgc tgaacggaat
      541 ggcaaaacgc cccgatagga attatgttat tgagaatgca aacattccag ttttcttcat
      601 ctttggaaaa cacgacaacc tgatttctat ggaggttgca gaggccttaa tggaaaggca
      661 caagaaagcc cgaaccgtta ttctaaacaa ctcgggccat atgggttttg ttgaggagaa
      721 ggatcaaaca accgctgcca ttacatcgtt tcttacccag gtttatagct gagcctgatt
      781 aagcacacgt gaattaacac tcctatgaag agctgaggtt gtaggttgac taacgctttg
      841 ctccattagc ccaattgctt ttagcaatgt ttcgtctata ttcctaatgt atggataaaa
      901 tccttcatta tcaataatat ttcgggcgat agttgctttt acttgtgcat ccagaatctt
      961 ttttgaaact gatatttcct tttcgtttct gggcacaccg ttttgctcgg caaactttat
     1021 aaagtcggag agcaggtcaa atttattcag gaacctatca accgagggaa agtcctttaa
     1081 acctcgagtt tctttccgat gctgatcggt gaagttgaag gcaaaccggt aaatgagatt
     1141 tcttcgggat acttgctgca ggtagggggt aattccgttg gtatcgagtg ggacaaaaat
     1201 atcgggcata atgcctcctc ctccataaac agtccttccg cctactgtgt agtattttag
     1261 cgagtcggcg aactgtatgc tatctgcaac ctcaaattct ccatgcaggt atcggcttgt
     1321 gatatcgttg aagtagttat cgtcgccagg tgtgtagggt ttttgaatgc atctacccga
     1381 tggggtgtag tagcgggcaa tggtgagcct tagggcagat ccatcggaaa aaaatatctg
     1441 ctcctgaacg aggcctttcc caaatgaacg ccttccaact atctgtcccc tgtcgttatc
     1501 ctgtatcgct ccggcaaaaa tttcacttgc cgaagcggaa aattcgtcaa ttaaaacctg
     1561 taaaggaata tcctggtact ttcctgttcc agtagagtaa acatcctgac gggttcggct
     1621 tttaccctgt gtgtaaacaa tcagctggtt tggctttagg aactcgttgg caattttagt
     1681 ggcagcatcc atgtaccctc caccattgct tcgcaaatca agtataagtt ttttcattcc
     1741 ctggctttcg agcttagcca ttgcatctag aaattcctta taggttgttt tggcaaaggt
     1801 tgtaatttta atatacccta tctcgggtgt taccatatag gcaatgtcca cgctacttat
     1861 tggaatttta tcccttgtta tctcgaaatc aataagctcg tcaacgtctt ttcttttgat
     1921 ttcaacctta acctttgtcc cggtagggcc tttcaagaga atggggatgc tgtccatcgg
     1981 aaattttact cccgcaatca gctttccgtt gacctttaca atcctgtcac cggccaaaat
     2041 tccgagcctt tccgaggggc ctccctttat ggtattaaca actactgctg tgtcattcga
     2101 catgttgaat tgaattccaa ttccgtcaaa gcccccttcg agaggttcgc ttacagattg
     2161 cagctccgaa gctggtatgt aaatggagtg tgggtctagg tttttaagta tagcaggtat
     2221 agtgttttcg accagggtag atacagaaat tgaatctaca tactcctctt ctatgtagct
     2281 tatcacgctg ctcagcttat cagttcggtt ataaaccagc gttccgggct tttggctttt
     2341 cccattaaat cgttgaccaa taagtattcc tgccgccagc actagggcta tgactatagg
     2401 atagtaaata tcctttttgc tattttggta tgccattcta ttcaagtaat ttattctgaa
     2461 gttattggta ttaaaaccat caggttgtac tcttttagaa atataacttt aaaaagccta
     2521 aagttcctct atgtcgagtt ttaccacatc aattcctgct ctactcaaaa gcgaaaggcc
     2581 atcgggattg tggtattcgt cgcagaaaac tacccgtttt atgcccgcct gaatgattaa
     2641 cttggcgcat tccatgcacg gcgacgaggt tacgtaaagg gttgctcctt ctgagctatt
     2701 tcctgattta gccaccttgg taattgcatt tgcctcggca tgaagaacat aaggcttagt
     2761 aagaccattc tcatcctcac agatgttttc aaaacctgag ggtgttccat tgtacccatc
     2821 agatattatc attctatttt tgacaataag cgctcccacc tgcctacgct tacagtaaga
     2881 gttttttgcc caaattttag ccatttccag ataacgtctg tcaaactgaa gctgtttggg
     2941 gtcattaaaa ctggtcgtct tattcatgaa gggctaacgt ttgcagcgtt tttgctacta
     3001 tagatttgaa ttatagctgt acagtaaacg gagaaaataa agcaactttt aagtaaaact
     3061 ggtggttgga taagtacccg gggccgggat cgaaccggca tggtattgct accactggtg
     3121 tttgagacca gcgcgtctac caattccgcc acccgggcaa taccgtttca aagaatttga
     3181 ggcttcaaag atataaagtt tatttcacag cacaataaca gggcttattc tgtagccatt
     3241 cttcatttaa agtaattgat tcactgttgc tgacctttaa attatgtgat ggattataaa
     3301 ccaactggtt gaattgttta acatttgcct gcagaactaa agagcttcgc tgattggttt
     3361 aaacccctcg ccaagaatct cgttggcatc aataactgta ataaaagcct taggatctat
     3421 ttcacgaatg taatccttaa gcataaaaac ctctctgcgg gaaacgttcg tgaaaatcaa
     3481 ctttttttcc tcaccgctat acattccctt aacgctaata agcgttccgc ctctttcgag
     3541 atcgttgatg atttttgacc tcacctcctc gtaacggtcg ctaatgataa acagggcttt
     3601 atcgtagctg gctccctcta tggtcatatc tatcaccttc cccgttatgt agatcaccac
     3661 ccatgagtag agagggattt tccaatcctt aaatgctaca agtgctatta gaacgatggt
     3721 agagtcaaca taaatcatca gctgtcctaa tggtaacctg gtgtactttg caattatcat
     3781 agcaataatg tcggaaccgc cactggttgc ctttgattta aaaatcagcc caagcccgaa
     3841 gccaattaga acccctccaa aaattgacga aagtagcata tcgtccacaa gaggatggta
     3901 gccgtaaatc atgtgaaggg tatctataaa aaccgacgac aaaataa
//
LOCUS       DAOOQX010000103         3946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_27325_length_3946_cov_2.840658, whole genome shotgun sequence.
ACCESSION   DAOOQX010000103 DAOOQX010000000
VERSION     DAOOQX010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27325_length_3946_cov_2.840658"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PLX53_02520"
     CDS             complement(<1..499)
                     /locus_tag="PLX53_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOY71983.1"
                     /translation="MRIETDYLGPMPIPDDALYGIHSQRAKENFPDITPFHKEWYQAM
                     GLVKQACYLTYVDFAKAVKNEPELSERLIDLIPDDILHAMISAAVEIQNGNYFEHFIV
                     PAFQGGAGTSINMNVNEIIANVALLKVGRKPGDYQLIDPIEHANIYQSTNDVVPTALR
                     VAATRL"
     gene            complement(647..1729)
                     /locus_tag="PLX53_02525"
     CDS             complement(647..1729)
                     /locus_tag="PLX53_02525"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOY71984.1"
                     /translation="MKAIEFATILTILAGCTNGQKNQPRVVNSQPPIADLRLIRIEEP
                     QDGAIFPLGDNIHISIKQTGNVAPDSVVLSVNGARVERLGGLTYELSTGGLKMGTQSI
                     SLAAWKSGSRQIAGVSVKLKSNFKPEKLTYRVVRSYPHDGRAYTQGLFYHDGYLYEGT
                     GQEGESSLRKVELETGKVLRSINLGREYFGEGIALLKGMIYQLTWTSGIGFIYDFNTF
                     NNLGSFTYATQGWGLTTNGKELIMSNGSNIISFMEPSGFSEVSRIEVFDDVGPVKMLN
                     ELEYINGKIYANVYLTDRIVIINAETGAVTADVDLKGILSPSDKTGSEDVLNGIAYDF
                     QGNRLFVTGKYWPKLFEVKLVPVGKR"
     gene            complement(1827..2246)
                     /locus_tag="PLX53_02530"
     CDS             complement(1827..2246)
                     /locus_tag="PLX53_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71985.1"
                     /translation="MFRIAVDFDGTIVENAYPAIGKPITFAFETLRELQKRGFAIILW
                     TVRSGKLLDEAVEFCRQNGVEFYAVNRNYPEESFDGEQPRKIEADVFIDDRNLGGFPG
                     WGKAFEMLCPEELSHELEKKALSQYKKKGFLNRLLGH"
     gene            complement(2394..2660)
                     /locus_tag="PLX53_02535"
     CDS             complement(2394..2660)
                     /locus_tag="PLX53_02535"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOY71986.1"
                     /translation="MKRNGSSTSISNGTFNGLIVNTPASTNVYMLGPSTPMCDVMFSY
                     PQVKGLFGSVFEPHDQKTIQIIAAGGGTRQFKTCMRKVYRLRHE"
     gene            complement(2812..3591)
                     /locus_tag="PLX53_02540"
     CDS             complement(2812..3591)
                     /locus_tag="PLX53_02540"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HOY71987.1"
                     /translation="MAINNQKKEEIKNIIKESIRSKFKHYKPETLSMPFHYRLLGKDR
                     MALYSFIQSLNTTFGTSIFEPVAVALAQDKFTKVRAQYSVGNTISQGCQQAIQTIMNS
                     LSTEKSPCKPEELEAIRNAALAGNLVKIKTVKVDLFLEDRNGEQYLFDLKTAKPNISN
                     FKDFKRTLLEWAGIIYTSNSNADVNTLIAIPYNPYEPKPYERWTIKGMLDDKYELMVG
                     EEFWNFLGGDDAYNDLLDCFEIAGIELRPEIDGYFSNFTRQ"
     gene            complement(3591..>3946)
                     /locus_tag="PLX53_02545"
     CDS             complement(3591..>3946)
                     /locus_tag="PLX53_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOY71988.1"
                     /translation="PPYVGLIDYHEQHAYAYDLFGFKRRDELEIGPLYKGQGKDAKDN
                     YVSGISEVLNNAKRFMVDDFNVFLVANDKYNLYPQIAQNAGMRIVNTYKRPVLNRTEK
                     DKNPYSETIFHLKKI"
BASE COUNT         1048 a          854 c          835 g         1209 t
ORIGIN      
        1 gcagcctggt tgctgctacc cgtaaagcgg ttggaactac atcgttggtt gattggtaga
       61 tgttggcatg ttcaatggga tcgataagct ggtaatcacc aggctttcta ccaactttta
      121 gaagggcaac gttggcaata atctcgttca cattcatgtt tatgctggtg cctgcaccac
      181 cctgaaatgc gggaacaatg aaatgctcaa agtagttgcc gttttgtatt tctacggctg
      241 cagaaatcat tgcatgtagg atatcatcgg ggataaggtc aataagcctt tcggaaagtt
      301 ccggttcgtt cttaacggct ttggcaaaat caacataggt taagtaacag gcctgcttca
      361 caagccccat tgcctggtac cattccttat ggaatggcgt aatatcggga aaattctcct
      421 ttgcccgctg cgagtgtatt ccatataggg catcgtcggg aattggcatt gggccaaggt
      481 aatctgtttc tatgcgcatc tacctgaatg ttagattttt ttgatggtta catggattct
      541 gcctgtaaaa aaacttagta cgcataaacc atcttaaatg ctgaaatcaa aaaaaataat
      601 ttagatgaaa taatagtagc taaaagaatg tggcttaaag ctgctgctag cgttttccaa
      661 ccggaacaag cttcacctcg aaaagttttg gccagtactt acccgtaacg aatagtcgat
      721 ttccttgaaa atcgtaggcg attccattta gcacgtcttc acttccggtt ttgtccgacg
      781 ggctgagaat ccccttaaga tcaacgtcgg ctgtaactgc gcctgtttcg gcgttaataa
      841 taactatcct atcggtaagg taaacgttgg cgtaaatttt tccattaata tactccagct
      901 cgttgagcat ttttacaggt cctacatcgt cgaaaacctc aattctactc acttccgaaa
      961 atcccgaagg ttccatgaat gagatgatgt ttgagccgtt gctcatgatt agctctttac
     1021 cgttggttgt taacccccat ccctgggtgg cataggtaaa tgagccgagg ttattaaacg
     1081 tattaaaatc gtaaataaaa ccaataccgc tagtccatgt aagctggtaa atcatgccct
     1141 ttaggagggc tattccctca ccaaagtact ccctgccaag gttgatgctt cttaacacct
     1201 tgccggtttc cagctcaacc ttacgcaagc tcgattctcc ttcctgtcct gtaccctcgt
     1261 aaagataccc atcgtggtaa aatagaccct gagtgtaagc cctgccgtcg tgggggtaag
     1321 agcgaaccac cctgtaggtt agcttctcgg gtttaaagtt cgacttcagc ttaacggaaa
     1381 caccagctat ttggcgtgag ccgctcttcc atgcagcgag tgatatgctt tgtgtcccca
     1441 tctttaagcc accggttgaa agttcatagg ttaaacctcc caaccgttca accctagcgc
     1501 cgttgaccga tagcaccacc gaatcggggg caacattgcc ggtttgtttg atggagatat
     1561 ggatattatc gcccagggga aatatagcac catcctgggg ttcttcaatc cggataagcc
     1621 ggaggtctgc tatgggcggt tggctgttta ctacccgtgg ctgattcttt tggccattgg
     1681 tgcatccagc caaaatagtg agaatagtgg caaattcgat cgctttcatg aacttataca
     1741 ttttagctgt cgggaaatta tgtcgttatg aagtgatttg ctttaccgta tgccaaactg
     1801 ttaggtgtta atctggttaa cttgggttaa tgtccgagta acctgtttag aaagcctttc
     1861 tttttgtatt ggcttaatgc ttttttttca agctcatggc ttaattcttc cgggcaaagc
     1921 atctcaaaag ccttacccca tccaggaaat ccaccaaggt ttctatcatc aataaacaca
     1981 tcggcctcaa ttttgcgggg ctgctcgccg tcaaacgatt cttcggggta gttacggttt
     2041 accgcataaa attccacacc gttttgccta caaaattcca cggcttcatc gaggagttta
     2101 cctgaacgca ctgtccagag tatgatggca aagcctcttt tttgcaactc gcgtagagtt
     2161 tcaaaggcaa aagtaatggg cttaccaata gctgggtagg cgttctccac aattgttccg
     2221 tcaaagtcaa ctgcaatcct gaacatttca attcgtttga taggccaaat ctacgttaaa
     2281 tttagaaaag aagatgcccg gcacttagaa aatcaaacct gttgaagcga cagctaaagg
     2341 tctgctggtc gaacccctaa gctgtgcgct tattgtaaaa taatctggct ggcttactca
     2401 tgcctcaacc tgtaaacctt cctcatgcag gttttgaact ggcgagtgcc gcctccggca
     2461 gcaataattt gaatggtttt ttggtcatgg ggctcaaaaa ccgacccaaa aagcccctta
     2521 acctgagggt agctgaacat tacatcgcac atgggtgttg aaggccccag catgtaaaca
     2581 ttggtgcttg ctggcgtatt gacgatgagg ccgttaaaag ttccgttaga aatgctggtt
     2641 gagcttccat tccttttcat ggagcaaggg ggtcaattat ccgaaatacc taagtagcat
     2701 gcccgagaat atccctaaat ttaagtatta aaatttgctt tgaaaatcgt aagttgggtt
     2761 cagtttcaac caagcaatta ctgtggaaga gtgcatggcg catccaaatt gttattgtct
     2821 ggtaaaattg gaaaaatacc catcaatctc tggtcttaat tcaattccag caatttcaaa
     2881 gcaatctaat aggtcgttat aagcgtcatc tcctccgaga aagttccaaa attcctcacc
     2941 taccattaac tcgtacttat cgtcgagcat gcccttaata gtccatcgct cgtagggttt
     3001 aggctcgtat gggttgtagg gaattgcaat tagcgtgttt acatctgcat tgctattgct
     3061 agtatagata atgccagccc attccaaaag agttctttta aaatctttaa aattactgat
     3121 attcggctta gcagttttta ggtcgaatag gtattgttct ccgtttcggt cctcaaggaa
     3181 taagtcaacc tttacagtct taatttttac aagattacca gccaaagcag cattccttat
     3241 ggcttcaagt tcttcgggct tgcaagggga tttctccgtt gacaagctat tcattatggt
     3301 ttggattgcc tgctgacaac cctggctaat agtgtttcca acagagtatt gtgcacgaac
     3361 cttagtaaat ttatcctgtg ctaaagcaac agcaaccggt tcaaaaattg aggttccgaa
     3421 agttgtgttg agtgattgga taaaagagta aagcgccatt ctatccttac ctaatagcct
     3481 gtaatgaaag ggcatactta gggtttcggg tttgtagtgc ttaaatttac tacggatgct
     3541 ttctttaata atattcttaa tttcttcttt tttctgatta ttgatggcca ttatattttt
     3601 tttaagtgaa aaattgtttc agagtatggg ttcttatcct tttcagttct atttaaaact
     3661 ggacgtttat aggtgtttac aattctcatt cctgcgtttt gtgcaatttg tgggtagagg
     3721 ttatacttgt cattggcaac caggaaaacg ttaaagtcat caaccatgaa tcgttttgca
     3781 ttgttgagaa cttccgaaat tccactaacg tagttatcct tggcatcttt gccttggcct
     3841 ttatacaatg gaccaatttc caactcgtct ctacgcttaa aaccaaacaa atcgtatgca
     3901 taggcatgct gctcatggta atcaattaat cccacgtagg gtggtg
//
LOCUS       DAOOQX010000104         3907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_27658_length_3907_cov_3.195483, whole genome shotgun sequence.
ACCESSION   DAOOQX010000104 DAOOQX010000000
VERSION     DAOOQX010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27658_length_3907_cov_3.195483"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="PLX53_02550"
     CDS             <1..1545
                     /locus_tag="PLX53_02550"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOY71989.1"
                     /translation="MPGIEFEFMLANGLVPTITLEEINAYIASMVTDKNVALTLMAPK
                     KDDIQLPSEEAILAAVKEVKSETLQAYVDNVVMKPLVESIPTPGQIKKVNDKNIFGTT
                     ELTLSNGVKVILKPTDFKEDEVKLDAFSKGGLSLADLATLPSATLATDMVVNAGVGEF
                     SSVDLEKLLAGKMVRVNPVFGEDFEGFQGSASPKDFETMLQLVYLYMTAPRQDQENFN
                     TYLDRLKAFFANAAADPRMAFRDSVNTAMANHNPRVMPINTDFLSKVDFNKCYSFYRD
                     RFADASDFTFIFTGNINVDEAKALTAKYLGALPVVKRKDIPRDNGVRPPKGKVQNYFT
                     KPMQTAKSSVFVGFTGNAEYNLKNHVMAEYLTSILRTRYLEEIREKEGGTYGVGVNIS
                     IDNFPTQSFTAGIQFDTDPKLRDKLIGIVYAEIEKIKQNGPTPDDLNKTKEFLMKEYE
                     QNQRENSYWSDAIRMKYEDGVDFNTEYLNVVKSTSVESLKDFAQKLFNQNNVVEVVMT
                     SEEAQK"
     gene            complement(1705..>3907)
                     /locus_tag="PLX53_02555"
     CDS             complement(1705..>3907)
                     /locus_tag="PLX53_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOY71990.1"
                     /translation="SPAIISGNLISEMGFKGLIFTDALNMKGVADYFSPEDINLKALL
                     AGNDVLLFPSEVSRSIDKIEKMVRRGQIPEEVINIKCRKVLMAKYAAGLSSYKPIEVL
                     NLYSDLNRVSSEVIRRKIISLALTVVHNNNSFIPVKRLDTLRIAYLEIGCNRGDAFRE
                     QLELYTAIHTFSIDPASPAEEFDSLLNMLEPYNLVIAGYHATDSRFSMNYGVTCQAAN
                     FLFDLAFSRAVVLDIFGSPYAISKFYNPESFRAIIVSYDNSFDAQNLSAQLIFGGRFA
                     TGRLPVTASAWYPAGYGVTTINPIRLGYALPEEVGINSLLLKEVDSLANSAIELAVAP
                     GMQILAAKDGVVFYHKSFGKPTYLSSDSVDVRMLYDVASVTKVVATVPSVMRLYDEQA
                     LELDNNLGAYLDLKEFPDKSAIRLSDLLRHQSGLKSWIPFNLKTLTTMIPNIPLWQEK
                     LSTDYPFQLGSKQFFSRYTVPNPVFYCDKESRDFPNRCAEGLYSAYFMADTIFSAINR
                     SELSQPGKYVYSDLGFMYLQRVVEGITQTGIDRYCEDKFYSRLGMSYTLFKPLTRFDR
                     DRIVPTEVDLIFRRQLVWGDVHDPAAAMLGGVSGHAGIFSTANDLAKLMQMFLQDGTY
                     GGERFFRPGTVEQFTTAPIEGNSSDNRRSLGFDKPASTGPSPAGSLASSQSFGHTGFT
                     GTTVWADPENGLVYIFLSNRVYPDASNTKLAEMNIRTRIHDVFYRAIGVAK"
BASE COUNT         1080 a          913 c          924 g          990 t
ORIGIN      
        1 atgccgggca ttgaatttga attcatgctt gcaaacgggc ttgttcctac cattaccctt
       61 gaagagatca acgcctacat tgccagtatg gttaccgata agaatgttgc gttaaccctt
      121 atggcaccga aaaaggatga tattcagctg ccaagcgagg aggccattct ggctgctgtt
      181 aaagaggtaa aatccgagac gttgcaggca tacgttgata acgtggtgat gaaacctctg
      241 gttgaaagca ttcctactcc aggtcaaata aagaaggtaa acgacaaaaa catctttggc
      301 accacggagc ttacgctctc caacggagtt aaagtaattc ttaaacctac cgacttcaaa
      361 gaagacgagg ttaaactgga tgccttcagc aagggaggct tgtcgctggc cgatttagca
      421 acgctaccat ctgctaccct ggctaccgat atggttgtaa atgctggcgt gggtgagttt
      481 tcgagcgttg acctcgaaaa gctgctagca ggaaaaatgg ttcgtgttaa ccctgtattt
      541 ggagaggatt tcgagggatt tcagggaagc gcatctccaa aggattttga aaccatgctc
      601 cagctggttt acctttacat gacggctccc cgtcaggacc aggagaactt caacacgtat
      661 ttagacaggc taaaggcatt ttttgcaaat gccgctgccg atcccagaat ggctttccgt
      721 gatagtgtta acactgccat ggctaaccat aaccctaggg ttatgccgat aaatactgat
      781 tttctgagta aggttgattt caacaagtgc tacagcttct accgcgaccg gtttgccgat
      841 gcttccgatt tcacctttat ttttaccggc aacatcaacg tggatgaagc taaagcgctc
      901 acagctaagt atcttggtgc actacctgta gttaagcgaa aggatattcc cagggacaac
      961 ggtgttagac cgcccaaggg aaaggtgcaa aactacttca ccaaacccat gcaaactgct
     1021 aaatcatcag tgtttgtagg atttactggt aatgccgagt ataacctcaa aaaccatgtg
     1081 atggccgaat accttacctc tattcttcgc acacgctacc ttgaagaaat tcgtgaaaag
     1141 gaaggcggca cctacggcgt aggcgtaaat atcagcattg ataactttcc aacccagtca
     1201 tttactgccg gcatccagtt tgacaccgat cccaagcttc gcgataagct cattggaatt
     1261 gtgtatgccg aaattgaaaa gattaagcag aatggcccta ctcccgacga ccttaataaa
     1321 acaaaggaat tcctgatgaa ggagtacgag caaaaccaaa gagaaaacag ctactggtcc
     1381 gatgccatta ggatgaagta tgaggatggc gttgatttta atacggaata cctcaacgtg
     1441 gtgaaatcaa ccagcgtgga gagtctaaag gactttgcac agaagctatt caaccaaaac
     1501 aatgtggttg aggttgttat gacatccgaa gaggcacaaa aatagcaaca gctagtactt
     1561 gaaatggaga aaagacgctt aaacaggcgt cttttctttt tagtcattta ttattccata
     1621 aagaacagat aatttaaggg gctactttga gcgtacacca tgcttcgcaa tctttcattt
     1681 ttcagattgt gagttggcta cccctcattt tgctacccca atagccctgt aaaaaacatc
     1741 gtgaattctg gtgcggatat tcatttcggc caacttggta ttcgaagcat cggggtaaac
     1801 cctgttcgaa aggaaaatat aaaccaaccc attctccgga tcggcccaga cggttgttcc
     1861 ggtaaatccg gtgtgtccaa aactttgtga agaggccaaa cttccggctg gtgatggacc
     1921 cgttgatgcg ggtttgtcaa atccaagaga gcgtctatta tcgctgctgt tgccctcaat
     1981 tggggctgtg gtgaattgct ccacagtgcc cggacggaaa aagcgttcac ccccataagt
     2041 tccgtcctgc aggaacatct gcattagctt agccaggtca ttggcagtgg agaagatgcc
     2101 ggcatgtccc gataccccgc ccagcatggc tgctgccgga tcgtgcacat cgccccatac
     2161 cagctgacgt cggaaaatca aatccacctc cgttggaaca attctgtccc ggtcaaaacg
     2221 ggttaaaggc ttgaagagcg tgtagctcat tcccaaccgg ctgtagaatt tatcctcgca
     2281 gtatcggtca atacccgtct gggttatgcc ctcaaccacc cgctgcaggt acataaaccc
     2341 taagtcgcta taaacgtact tgccgggttg ggagagttct gagcggttaa tggcgctaaa
     2401 aatggtatcg gccatgaagt aggcagagta tagtccttcg gcacagcggt ttggaaaatc
     2461 cctcgattcc ttatcgcagt aaaaaaccgg atttggcact gtgtagcggc tgaaaaattg
     2521 cttgctccca agctgaaacg gataatcggt gctaagcttc tcctgccata gtggtatatt
     2581 gggaatcatt gtggtaaggg ttttcaggtt gaaaggtatc catgatttaa gtcccgactg
     2641 atgcctgagc aggtcggata gccttattgc tgacttgtcg gggaattcct ttaaatcgag
     2701 gtaggctccg aggttattat caagttcaag tgcctgctcg tcgtagagcc tcatgaccga
     2761 tggaactgtt gccaccacct tggttaccga tgccacatcg tagagcatac gaacatcaac
     2821 cgaatcgctg gaaagatagg tgggctttcc aaaactttta tggtagaaca ccacgccatc
     2881 cttagctgcc agaatttgca ttcccggggc aacagcaagc tcgatagctg aattggccaa
     2941 ggagtcaacc tccttaagca gcagtgagtt tatgcctacc tcctcaggca aggcgtaacc
     3001 taagcgaatg gggttgatag ttgttactcc atatccggca ggataccagg cacttgcggt
     3061 aacaggcagc ctcccagtag caaaccggcc tccaaaaatc aactgggctg aaagattctg
     3121 agcatcaaac gagttgtcat acgaaacaat aatggcacga aatgattcgg gattatagaa
     3181 ttttgaaatg gcatatgggc ttccaaatat gtcgaggaca actgccctgc tgaaggccaa
     3241 atcgaagaga aaattagcag cctggcaagt tactccgtag ttcatagaaa atcggctatc
     3301 agtggcatgg tagccggcta ttaccaggtt gtatggctcg agcatgttca gcagcgaatc
     3361 gaactcttcg gcaggcgaag caggatcgat actaaaggta tgaattgcgg tgtaaagctc
     3421 cagctgttcc ctaaatgcat caccccggtt acaccctatc tccaggtagg caatcctgag
     3481 cgtatcgagc ctttttacag ggataaagct attgttatta tgaaccactg ttagcgcaag
     3541 gcttatgatt ttcctacgta taacctccga ggacaccctg ttgagatcgc tgtaaagatt
     3601 tagtacctca atgggtttgt agctgctaag tcccgcagcg tactttgcca taaggacttt
     3661 tcggcatttg atgttgatga cttcctcggg tatctgtcct ctacgcacca tcttctcaat
     3721 tttatcaatt gaccggctca cctcggatgg aaaaagcagc acatcgttac ctgccagcag
     3781 agctttcagg ttaatatcct cagggctaaa gtaatcggct acccccttca tgttcagggc
     3841 gtcggtgaag atcagcccct taaatcccat ttcggaaata aggttaccgg aaataatggc
     3901 aggtgat
//
LOCUS       DAOOQX010000105         3906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_27672_length_3906_cov_2.768632, whole genome shotgun sequence.
ACCESSION   DAOOQX010000105 DAOOQX010000000
VERSION     DAOOQX010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27672_length_3906_cov_2.768632"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLX53_02560"
     CDS             complement(<1..213)
                     /locus_tag="PLX53_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71991.1"
                     /translation="MKAGIAPNGLMSAASVMKIFANSIRFAPAGRGPPAVIPRRADYD
                     AGATVGKSTWAQKPWRLDRIKDSFNMF"
     gene            143..484
                     /locus_tag="PLX53_02565"
                     /pseudo
     CDS             143..484
                     /locus_tag="PLX53_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
     gene            563..1642
                     /gene="ribA"
                     /locus_tag="PLX53_02570"
                     /pseudo
     CDS             563..1642
                     /gene="ribA"
                     /locus_tag="PLX53_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
     gene            complement(1692..2282)
                     /locus_tag="PLX53_02575"
     CDS             complement(1692..2282)
                     /locus_tag="PLX53_02575"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOY71992.1"
                     /translation="MRQGLPGVEAQLLMSPSIRKNVNGNSSSNGQSRQSAVLILFFPD
                     EDETRLVLIKRQTYEGLHSGQVSLPGGKWEEVDGSLEQTALRETFEEVGVNPERVELV
                     GRLTPLFIPVSNMMVQPVVGFTPEKPDFHPNLQEVDYIITVPVEQLFDQKNKSVRVIS
                     VNGSPITAPYYNVNGEFVWGATAMILAELEAVASRK"
     gene            2494..3873
                     /locus_tag="PLX53_02580"
     CDS             2494..3873
                     /locus_tag="PLX53_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71993.1"
                     /translation="MKKIILISTLALLVASTGCVSKKYLKRGIKYEEAGLWELAAESY
                     IQSLTSKRDNLDAIVGLKRTGQKVIDEKCYKSIAAYESDNLKEAVYDYLDAVDFSKRT
                     SSLGVELMVNSRATDYFKDAKPRYIETLYAQAQQQMDAEKFQQAEALLDEIKKLEPDY
                     GNVKEMLKVSKCEPLYRQGKQLMESGYYRKSYGNFNQIVTEFGSYKDAAMLMGDALQK
                     AIITIKVNNFTGGYCTKALTQRIQSSVVASVNTLNNPFVKIVDSDNTIQIIEEQQRSL
                     TQGSDIEVGKILAAKAILTGEVLECKQDAGRLNKSDKRGYLKEVTKVKNPVTGETENK
                     VEYKKVSYEVYSIQNRVTFSLKFQLTSTETGAVMVSDIARSQQADETTYAVFNGKPEK
                     LVPGYWENINKDSPKDFVSDNPADVSALQKMLQARREISSVDQLTDKAIAALSKQVSE
                     RINRYNPEQ"
BASE COUNT         1083 a          999 c          918 g          906 t
ORIGIN      
        1 aaacatatta aatgaatctt ttattcggtc aagccgccat ggcttttgcg cccaggtcga
       61 tttgccgacg gtcgcgccgg catcgtagtc tgcgcgtcgc gggatcacgg cggggggccc
      121 ccggcccgca ggagcaaatc gaatggaatt cgcgaagatc ttcatcacgc tggcggcgct
      181 catcaacccg ttcggcgcga tccccgcctt catcggcctg acccaggggc aatcgtccct
      241 cgagcgccgc cgcaccgcgc gcaccgctgc gctcgcgacc gtcctcgtca ttaccgtgac
      301 cgcgctcgcc ggtcagtacc tgctggcggg cttcggcatc accgtgccct cgctgcaggt
      361 cggcggtggc gtgctgctct tcatcgtcgc gatctcgatg ttcaacgccc agccggcgcc
      421 gagccgcacc acgccggagg aaaccgacga ggccgccgag cgcgccaaca tcgcggtggt
      481 gccgctgacc atcccgctac tgaccgggcc gggcacggtc agtacggtga tcatctatgc
      541 cgaccgctgc ctactgtcgg cggacttcat ggccaagcac gggaggggtt taatttgtgc
      601 tcctctacct gagaaacgat gcgatgagct ggaactcgac cttatggttg ataggaacac
      661 tgccccccac aacaccgctt tcaccgtttc tgttgacttg cttggacatg gttgcaccac
      721 agggatatcg gcatccgata gggcaaaaac cgtcaaggct cttgttaacc ccagcacaac
      781 ccctgaagat ttgggacgcc caggccacat atttcccctc agggctaagg acaaaggtgt
      841 acttagaagg ccaggacata ccgaggcggc cgttgacctc acccgtttgg cagggttaac
      901 accgggagga gtgctggtgg agattatgaa tgacgatgga actatggcca gactcccgca
      961 gctgctcgag attgcaaaaa aatttgacct aaaaataatt tccataaaag accttattgc
     1021 cttcctgctc cattccgaaa ccatcatcga gagaggtttc aaggttaaac ttcctaccga
     1081 gttcggctac ttcgacctta ttcccttccg ccagaaatca aatggacttg aacatgttgc
     1141 cctaatcaag ggggaatggg aggccgacga gcctatactg gtaagagtcc actcctcctg
     1201 tgttactggt gatatatttg gctcctacag atgcgactgt ggaccacagc tacaccgcgc
     1261 catgcagatg attgaacatg agggaaaagg agttttggtt tacctaaacc aggagggcag
     1321 aggaattggc ctgttcaaca aaattcatgc ctacaagcta caggaagaag ggctggacac
     1381 cgttgaagca aatatcgagc tgggctttca acccgatgaa cgcgattatg gcgtaggagc
     1441 cagtatcctc cacgaggttg gtgttagcaa catgaagctc attaccaata atccgaaaaa
     1501 gatgaggggt ctcgagggtt acggtttaaa aattgttgaa aatattcccc tggttatccc
     1561 atcaaacccc ttcaacgagg tgtacttaaa aaccaaggtg gagaaaatgg gccatatcaa
     1621 cctttttgga cctaagcagt agcctctcca aattaatcta ccactacctc caagccaaaa
     1681 gccccataga cctactttcg ggaagcaacc gcttcaagtt ctgccagaat catggctgtt
     1741 gcaccccaca caaactcgcc attcacatta tagtagggag ctgttatggg tgaaccatta
     1801 accgatataa ccctcaccga tttatttttt tggtcaaaaa gctgctccac cggtacggtt
     1861 atgatgtagt caacttcctg taggttggga tgaaaatcgg gtttctcggg cgtaaacccc
     1921 accactggct gcaccatcat gttgctaacc ggaatgaaca aaggcgttag cctgccaacc
     1981 agctccaccc tttcaggatt cacacctacc tcctcaaatg tctcccgcag agcggtttgt
     2041 tccagcgatc cgtcaacttc ctcccatttg ccacccggaa ggctcacttg tccgctgtga
     2101 agcccttcgt aggtttgcct tttgatgaga accaaccttg tctcgtcttc gtccggaaag
     2161 aataggatta gcactgcact ttgacgggat tgcccatttg agctgctatt tccattcacg
     2221 tttttacgaa tggagggcga catcaacagc tgtgcctcta caccaggcaa accttggcgc
     2281 attttaccct tcaaactgct tataaaatga gaaaacgcac atgcagaaaa actatacata
     2341 gcctgctaag ctccttaaaa tatcattcca ctacaaccta tcaaaggtaa aaaaattgaa
     2401 tttatcatta cccaacattg cgggtatatc atatttcctt aactttacgc tagaaaggtt
     2461 tttccatatt ggttcatatt agtgcagcta aatatgaaaa agataatttt aatttcgact
     2521 ctggccctgt tggttgccag cacagggtgc gtatctaaaa aatacctcaa gcgaggcatt
     2581 aagtatgagg aagctggact ttgggagcta gcagccgaat cgtacatcca atcgctaacc
     2641 tcgaaacgtg acaacctgga tgcaattgtt gggcttaaac gaacaggtca gaaagtgatt
     2701 gatgaaaagt gctacaaatc aatagccgct tacgaatcgg acaatttgaa ggaagctgtg
     2761 tacgactacc ttgatgcggt ggattttagt aaacgaacat cctcgctagg cgttgagcta
     2821 atggttaata gtagggctac cgattatttc aaggatgcca aacctagata tattgaaacg
     2881 ttgtatgctc aagcccagca gcagatggat gctgaaaagt ttcagcaagc tgaagccctg
     2941 cttgatgaga ttaagaagct ggaacccgac tatggaaacg tgaaggaaat gctgaaggta
     3001 tctaaatgtg agcccttata ccgacagggg aaacagctga tggaaagtgg ttactaccga
     3061 aaatcctacg gaaattttaa ccagatagta accgaatttg gcagctacaa ggatgccgcc
     3121 atgctgatgg gtgatgccct ccaaaaagcc attataacca taaaggtcaa caattttaca
     3181 gggggttact gcactaaagc cctgacccaa cgcatacaaa gttcggtggt tgcttcagta
     3241 aataccctta acaacccctt cgttaaaatt gttgactccg ataataccat acaaatcatc
     3301 gaggaacaac aacgtagcct tacacaaggc tcggacattg aagttggtaa aatactggct
     3361 gcaaaagcaa ttctaacagg cgaggtgctt gaatgtaagc aggatgctgg ccgtctgaat
     3421 aaatccgaca agcgaggcta ccttaaagag gtaaccaagg tgaaaaatcc cgtaacaggt
     3481 gaaacggaga acaaggtgga gtataaaaag gtttcctacg aggtttacag cattcagaac
     3541 cgggtaactt tctcgctgaa atttcagctt acatcaaccg agaccggtgc cgtgatggtt
     3601 tccgatattg cccgaagcca gcaagcagat gaaaccacct atgctgtatt caacggcaag
     3661 cccgaaaagc ttgtccccgg atattgggaa aatattaata aggattcacc aaaagatttt
     3721 gtatccgaca acccggccga tgtttctgca cttcaaaaaa tgctacaggc ccgcagggaa
     3781 atatcctcag ttgaccagct aaccgacaag gccattgctg ccctgtccaa acaggtttcc
     3841 gaacgaataa accggtataa tcccgaacag taaaaactat ggttaaaaac agctactcga
     3901 cgattg
//
LOCUS       DAOOQX010000106         3890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_27817_length_3890_cov_3.507953, whole genome shotgun sequence.
ACCESSION   DAOOQX010000106 DAOOQX010000000
VERSION     DAOOQX010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27817_length_3890_cov_3.507953"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PLX53_02585"
     CDS             complement(<1..856)
                     /locus_tag="PLX53_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOY71994.1"
                     /translation="MSNKKFLVYIAVTVAMAFWALSFIWYKDAYVYYRPITLVFFRLV
                     ISAAFLYVFSLAMRKLERVKKGDLKLFFLLAFFEPLLYFIGESFGMTLISSTMAAVIV
                     STIPLLSPVGEFFMFHRRISTTNLMGIIASMLGVCLVIFHNGFGQVDVNFTGVALMFL
                     AVFSTVGYSLIVGKLTVKYNVYSIITYQNTIGALYFMPLFLIFDLNHFQTVGISWQAM
                     VPVIKLGIFASTFAFMLFTYSIKHLGITRANTFSNAIPVLTAIFAYFLLDEALTPVMI
                     AGILVVVSG"
     gene            complement(1150..>1955)
                     /locus_tag="PLX53_02590"
     CDS             complement(1150..>1955)
                     /locus_tag="PLX53_02590"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOY71995.1"
                     /translation="ITDSIRYASRIQSAVMPRTDTLATFLTDYFIYFQPKNIVSGDFF
                     WVTRLNDGRIAIAVADCTGHGVPGAFMSILGITLLKEIIAYQQVNTAGDLLDKLRQMV
                     IAALNQSGQVQDSHDGMDMGLLVVDAKGRKAEYAGAYMPLLVSRNSELPEVSAGVQPE
                     TANGWSIYEIKGDKMPVGYHLAGERPFTTHRFDLLSNDTFYLMSDGYTDQFGGALNQK
                     LMMVNFKKNLLGLQTLSLSRQNEELQKQLIEFQGTQKQVDDILVMGFRL"
     assembly_gap    1956..1965
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1966..3454)
                     /locus_tag="PLX53_02595"
     CDS             complement(<1966..3454)
                     /locus_tag="PLX53_02595"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY71996.1"
                     /translation="MNSTEFHRNILLVLILLTFSLSYSQAQSDTVRIGEMLQRAEVLR
                     TTNPDSAIGLAIQALNIIDGSDNKELMVRVEHTLGNCYYSRNDFELALSHLKKSLQLA
                     TEEGRKFWVAMNLNRIGNVYQLKSDYGSALQAYTEALNINRSIDNKSQLARTLVNIGT
                     VYSITGSYKEAIENMLKALETYEQAKDYEGIAWASLSISRLFSRIDLYEKALQYAESS
                     LTHYRQLSNINGVTLAKTELANIYYHLGMYPKAKEMATDVLATNQQNQNIYGQAANHL
                     LLGIINFELGDYTSAKSNLLTSLELKRQVNDSIDLAKLSRYLGEVNLKSGNVAAGLES
                     LEQAMRIAQRQRVLTDQRDIFLSLSQAYRLRGDYRRSLDYFTRYSAVKDSINASQIAR
                     MEMQYDFDKREREQELISRQREAIQQAKLERQRMLTLFISIALLLAVALAGVIFYFYR
                     NKKKINALLVAQNQEILRQKEEIEAQKEEIESQRDLAERQRDQIAG"
     gene            3661..>3890
                     /locus_tag="PLX53_02600"
     CDS             3661..>3890
                     /locus_tag="PLX53_02600"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HOY71997.1"
                     /translation="MNVVKENTNEQTISIRAKVEQADYVEKVEKKLKDYRKNSNIKGF
                     RPGMAPAALIHRMYYKYVLVDEVTHIAFDAVM"
BASE COUNT          989 a          879 c          919 g         1093 t
ORIGIN      
        1 ggccagatac caccaccaga ataccggcaa tcatcacagg tgtaagggct tcatcgagca
       61 ggaaataggc gaaaatggcc gttagcaccg gaatggcgtt actgaaagtg tttgccctgg
      121 ttattcccaa gtgcttgatg gaataggtga aaagcataaa tgcaaaagtt gaagcaaata
      181 ttccaagttt tataaccggg accatagcct gccagctaat acctacagtt tggaagtggt
      241 tgaggtcaaa aataaggaat aaaggcatga agtaaagtgc cccaatggta ttctggtagg
      301 ttattatgct gtaaacgttg tactttacgg ttagcttacc tacaatcaac gagtacccaa
      361 ctgttgaaaa aaccgccaga aacatcaggg caaccccggt gaaattaaca tccacctgcc
      421 caaatccgtt atgaaagatt accaggcata cgcccagcat ggaggcaata attcccatga
      481 ggttggtggt ggaaatcctg cgatggaaca tgaaaaattc tccaactggg ctaagtaggg
      541 gaatggttga aacaattacg gcagccatgg tggaggagat aagggtcata ccaaaactct
      601 cgccaataaa gtatagaagc ggctcaaaga aggccagcag gaaaaacagc ttcaggtcgc
      661 ctttttttac ccgttctagc ttcctcatag ccagtgaaaa aacgtaaagg aatgccgctg
      721 aaattaccag acggaagaac actagcgtaa taggcctgta gtaaacgtag gcatctttgt
      781 accagataaa agaaagcgcc caaaaagcca ttgcaactgt gacggcaatg taaaccaaaa
      841 actttttatt agacattccc atacccttat gttatctatt gtttatccat tacgcaattt
      901 tctccacatt aacccattat gtccggctag cgtggtccgg ttagtttttt tacttgatgt
      961 acctcatgga tattcccctg ccattatttg agggtgaaag taaccaatgg tttatcgggt
     1021 tgcaaatgta gcagatttgg ctcacctaaa gctatcggat taatgcgatt ttggcattaa
     1081 actagggaat aggtaaaaac attgctgcgc tcgctttagc ggtagctatt ttatgggtat
     1141 gtttaccagc tataacctaa aacccatcac caggatatca tccacttgct tttgtgtgcc
     1201 ctggaattct attaattgtt tctgtagctc ttcgttttgc cttgataggc tgagcgtctg
     1261 gagaccaagc agattttttt tgaagttgac catcatcaac ttttggttca atgctccgcc
     1321 aaactggtct gtgtaaccat cggacataag gtagaatgtg tcgttggaga ggaggtcgaa
     1381 cctgtgagtt gtaaaggggc gttctcctgc cagatggtaa cctacgggca ttttgtcacc
     1441 cttaatctca taaattgacc atccgttggc tgtttcgggt tgaacaccag cgctaacctc
     1501 aggcaattcg gagtttctgg aaacaagtaa cggcatgtag gctccggcgt attctgcttt
     1561 ccttcccttg gcatcaacaa ccagcagccc catatccatt ccgtcatggg aatcctgcac
     1621 ctgtcccgac tggttcagag cggcgattac catctgccta agtttatcaa gcaaatcgcc
     1681 agcagtgtta acttgttggt aggctataat ctcctttagc agggtaatgc ccaatatgct
     1741 cataaaggcc cctggcaccc catgaccggt gcagtcggct acagcaattg ctatacgtcc
     1801 gtcgttcaaa cgggtaaccc agaaaaagtc accgctgaca atgtttttgg gttggaagta
     1861 aataaagtag tcggtcagga aggttgccag ggtatcggtt cggggcataa ctgccgactg
     1921 tatccggctg gcatacctga tgctgtcggt aatttnnnnn nnnnnggccg gctatttggt
     1981 cccgttggcg ttcagccagg tcgcgctggc tttcaatttc ctccttttga gcctcaattt
     2041 cctctttttg cctgagtatt tcctggttct gggccacgag tagggcgtta atttttttct
     2101 tgttacggta gaagtagaag attaccccgg ccagcgcaac ggcaaggagc agggcgatgg
     2161 agatgaagag cgtcaacata cgctgacgtt cgagtttggc ttgctgaatg gcctcacgct
     2221 ggcgggatat aagctcctgc tcccgctccc gcttgtcaaa atcgtactgc atctccatgc
     2281 gggcaatctg gctggcgtta atgctatcct tcactgctga gtagcgggtg aagtagtcta
     2341 gcgaccttct gtagtcgccc ctcaaccggt aggcctggga taggctaagg aatatgtcac
     2401 gctggtcggt gagcactctt tgcctttggg caatccgcat ggcttgttcc aatgattcca
     2461 gacctgctgc cacattgcca cttttcaggt tcacctctcc aaggtagcgg cttagcttgg
     2521 ccaggtcaat gctgtcgtta acctgtctct ttagctccag cgaggtcaac aggttgcttt
     2581 ttgctgaggt gtaatcgccc agctcaaagt tgataattcc cagtaaaagg tggtttgcag
     2641 cctgcccgta gatgttttga ttttgctgat ttgtcgccag cacatcggta gccatctcct
     2701 tagcttttgg gtacatgccc aagtggtagt aaatattggc cagctctgtt ttggccagag
     2761 taactccgtt tatgttgctt agctggcggt agtgggttag ggagctttcg gcgtactgca
     2821 gggctttttc gtaaagatcg attcgggaaa aaagcctcga tatgctcagc gacgcccatg
     2881 ctattccctc gtagtccttt gcctgctcgt aagtttccag cgccttcagc atgttctcaa
     2941 tggcctcctt gtagctaccc gtgatgctgt aaactgttcc aatgttcacc agcgttcggg
     3001 ccagctggct cttattgtcg atactcctgt taatattcag agcctccgtg tatgcttgca
     3061 gggcgctgcc gtaatcgctt ttaagctggt aaacattacc aatcctgttc aagttcatgg
     3121 caacccagaa ctttcgccct tcttctgtgg ctagctgcag ggatttctta agatgcgaaa
     3181 gagccagttc aaaatcgttt cgactgtagt agcaatttcc aagagtgtgt tctaccctta
     3241 ccatgagttc cttgttatcg ctgccatcga tgatgttaag tgcctggata gccaatccta
     3301 tggccgaatc cggattggtg gttcgtaaga cttcggcacg ctgaagcatt tctcctattc
     3361 tgaccgtatc ggattgagct tgggagtagc tcaaggaaaa tgtgaggaga ataagcacca
     3421 gcaaaatatt tcgatggaat tctgtggagt tcatagggca ttttttattt gataaatata
     3481 ccactttgac ggttttcatc aacatgtaca gcgtatttta taagcaactt ttagccccgg
     3541 ctaccagctt attacggtaa ccattttgtt gttacggggt gtttttctaa ttttgcgccc
     3601 tctgtatgga ggttaatttt ttaaatgtcc aaccattaaa tggttgtaat acaaaatatt
     3661 atgaatgtag ttaaggaaaa taccaacgaa caaaccattt ccattcgggc aaaggttgag
     3721 caggccgact atgtcgaaaa ggttgaaaag aaacttaagg actaccgtaa aaactcaaac
     3781 attaaaggtt ttaggccagg catggcacca gcagccctta tacataggat gtactacaag
     3841 tacgtgctgg ttgacgaggt tacgcatatt gcctttgatg ccgtaatgga
//
LOCUS       DAOOQX010000107         3887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_27840_length_3887_cov_3.427714, whole genome shotgun sequence.
ACCESSION   DAOOQX010000107 DAOOQX010000000
VERSION     DAOOQX010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27840_length_3887_cov_3.427714"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PLX53_02605"
     CDS             complement(<1..1001)
                     /locus_tag="PLX53_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY71998.1"
                     /translation="MKLSTLIKNTEDFFSERRLLYIILTGILIIDLTILVFSSGYYGG
                     ADTAGHYLSSHWAFAHPAYLLNHWSKPIFTLLSAPFAAVGFKTFQLFNILFGIATGYL
                     SYLVAKELKMKSPLLAIVICCFTPIFMISLFSGLTEILFAFVAILASYLLIKERYAWG
                     AVVVSFLPLIRYEAVVLLPVYAIFLINKKHYREILLMLTGMVIYSLLGALAGKNILWL
                     FTEFPHQNTVGVYGKGKFFHYILRSPGFFGIPNEIFFVTGLVAGLSLYLREKMEYSKE
                     FLLVVLPFLTYFFAHTVSWWLGIGGSQGQNRYMAAIVPFMAVMATRGLYLFAKMFLIL
                     "
     gene            complement(1277..2899)
                     /locus_tag="PLX53_02610"
     CDS             complement(1277..2899)
                     /locus_tag="PLX53_02610"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOY71999.1"
                     /translation="MNRAIPFLALLFFSCQDGNIKRAINSITSQEMGQDIMALSSDSL
                     LGRAPFTVGEERTVAYLQKRMAELKLTPAFEGSYIQSVPMVSITTMVPNTVDFTTPKG
                     ALKLKTDEEICLWSPTIKPNIKISSADLVFAGFGIDAPEYEWNDFAGLNVSGKIIVVL
                     VNDPGFYTHDSLLFTGNAMTYYGRWRYKFEEAERKGALGCLIVHEDAAAGYPWAVAGS
                     KSNTPILYMDSPELNNSRCVLNGWITLDAVRKLMGKCGYNYDSLKQLAVNRGFKPVGL
                     KACLSLTMENRQTKSASKNVAGYIAGSKNPDEFIVYTAHWDHLGIGKAVDGDSIYNGA
                     SDNAAGIAWMLSIAKAFQNSGERPERSVLFLSPTAEESGLFGSTYFVQHPPQEVGRMV
                     ACFNYDVALFLGKFSDVTITGPGHSNLDSLLRLAAKSQNRYVISDPNPEIGMIYRSDQ
                     LPFLKAGIPSMFAKGCIHQTELGKEKTQELINKYWLNTYHKPSDEFDPTRDNLDGLVE
                     DARLFYDMGNRLTSWNQYPKMSRTSEFYKRSR"
     gene            3137..>3887
                     /locus_tag="PLX53_02615"
     CDS             3137..>3887
                     /locus_tag="PLX53_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S9"
                     /protein_id="HOY72000.1"
                     /translation="MKYSKTILYLCSMTLLISNSGCNKANKPAAPEPLSINVSLSDSE
                     KAAGLLTPEILWKFGRVGEYALSPDGTMLVYAVTFYNLQENKGVTNLYVVSATGGEPL
                     KLTDETGSESAPQWSSDGQTILFLSTRGGSNQLWQVRPDGSELKQISGIDGDINGFSF
                     SPDNTKLVFAVDVKLDQQADDIYPDMPKAKVRIIDNLMYRHWNYWEDGRYSHLFVADF
                     ADGKISNARDINAGEKWDTPMAVDFDMTEVQW"
BASE COUNT         1062 a          857 c          873 g         1095 t
ORIGIN      
        1 agaataagaa acattttggc aaaaaggtag aggccacgag tagccataac tgccatgaag
       61 ggaacaattg cagccatata cctgttttgt ccctgagatc caccgattcc aagccaccat
      121 gataccgtgt gggcaaaaaa gtaggtaaga aagggaagaa ctaccagcag aaattccttg
      181 gagtactcca tcttctcgcg caggtaaagt gaaaggcctg ccactagccc cgttacaaag
      241 aaaatctcgt taggaatacc aaaaaaacct ggacttctaa gaatataatg aaagaattta
      301 ccttttccat atacccctac ggtattctga tgtggaaatt cagtgaaaag ccataaaatg
      361 tttttgccgg caagcgctcc caggaggctg taaataacca tgccggtaag cattagcaat
      421 atttcacggt agtgcttctt atttatcagg aaaatggcgt aaaccggaag tagaactacc
      481 gcctcatatc taatcagcgg aagaaatgaa actacaactg caccccaggc ataccgctct
      541 tttattagaa ggtacgaggc aagaatagca acaaaggcaa aaagaatttc ggttaatccc
      601 gaaaacaagc ttatcatgaa aattggggta aagcagcata tcacaatggc cagcaaggga
      661 cttttcattt tcaactcctt ggctaccagg tacgataagt agcccgtggc aatgccaaaa
      721 aggatgttga acagctgaaa ggttttgaac ccaactgctg caaacggggc gcttagaagt
      781 gtaaaaatcg gttttgacca gtggttaagc aggtaggccg gatgtgcaaa tgcccagtga
      841 gaagaaaggt agtgtccggc agtatcggca cctccgtaat acccactact aaaaaccaga
      901 atggttaagt caattatcag aatgccggta agaattatgt agagcaatcg cctctctgaa
      961 aagaaatcct cagtattttt gataagagtt gaaagtttca tgtcggttaa tttgattgtg
     1021 attgccggtt tttatgctat gtattttagc agttgagcaa gattgaagtt atgctcaatg
     1081 cgcccgtaaa aatagcataa tctacacttt ctttgccaac aaacaggtta ccattttatt
     1141 aatacaagat taaaactatt cctttttcag caatgtggcc tgtatggttc aactttacga
     1201 ctataatcca gcttctgctt tgagccgatt tttttaagat taccagatga gcggcaattg
     1261 ccaaggttag ctttggctag cggcttctct tgtagaattc tgatgttcga ctcattttgg
     1321 ggtattggtt ccagctagtt agccggtttc ccatatcgta aaacagcctt gcatcttcaa
     1381 ccaatccatc cagattatcc cttgtggggt caaactcatc agatggttta tggtaggtgt
     1441 taagccagta cttgttgata agttcctgtg ttttttcttt tccaagttcg gtttgatgaa
     1501 tgcaaccttt cgcaaacatt gatgggatac ctgcttttag aaatggtagc tggtcggatc
     1561 ggtaaatcat cccaatttcc ggatttgggt cggaaatcac atagcggttt tggctttttg
     1621 ccgccagcct caacaagctg tcgagattgg aatgcccggg accggtaatg gtaacatcac
     1681 tgaattttcc cagaaacaag gctacgtcgt agttgaaaca ggcaaccatt cgtccaacct
     1741 cttggggtgg gtgttgaaca aagtaggttg aaccaaaaag cccagactcc tcggcggttg
     1801 gtgacaaaaa taacaccgac cgttcgggcc tttcacctga attctgaaac gccttcgcaa
     1861 tggagagcat ccatgcaatg ccggcagcat tgtccgatgc gccgttgtat atcgaatcgc
     1921 cgtcaacagc cttgccaatt ccaaggtggt cccagtgggc agtgtaaact ataaattcat
     1981 ctggattttt gcttcctgct atgtagccag ccacgttttt agaggcagac tttgtttgcc
     2041 tgttttccat ggttaagctt aaacaagctt ttaggcctac aggcttgaat cccctgttta
     2101 ctgccagctg cttgagcgag tcgtagttgt agccgcactt ccccattagc ttcctgactg
     2161 catccagggt aatccatccg ttcaacacac acctagagtt attaagttcg ggtgaatcca
     2221 tgtagagaat tggagtgttg ctcttgcttc cggcaacagc ccatggatag cctgcagccg
     2281 catcctcgtg aacaatcaaa cagcctaggg ctccctttct ttcggcttcc tcaaatttat
     2341 agcgccaacg accgtagtag gtcatggcgt tgcctgtaaa cagtagcgaa tcgtgggtgt
     2401 aaaatcccgg gtcgtttacc agcacaacta ttatttttcc gctcacattc aatcctgcaa
     2461 agtcgttcca ctcatactca ggggcatcaa taccaaagcc ggcaaaaact aaatcggcac
     2521 tgcttatttt tatattgggc tttatggttg ggctccacag acatatttcc tcatcggttt
     2581 taagtttcag cgctcctttt ggagtggtaa agtcaacagt gttgggtacc atggtggtta
     2641 ttgaaaccat aggaacgctt tgaatatatg agccctcgaa tgctggcgtc agctttagct
     2701 ctgccatcct tttttgcagg taggccaccg ttcgctcttc cccgactgtg aatggtgccc
     2761 ttcccagaag gctatcggag gatagtgcca ttatgtcctg gcccatctcc tggctggtaa
     2821 tggagtttat cgccctttta atattgccat cttggcagga gaaaaacagt agggctaaaa
     2881 acggaatcgc tctattcatg atattaagtt tatatggtcg aaagcaaatg taatcaattt
     2941 atcagcattt atctgtagcg gatatttcga taccacaagt agattttggt ttaacttaac
     3001 tggtgggtta accccttgtg gtttgtaggt tgtttgaata tgtgcctatt tttttctaac
     3061 aacacctttt agctgtaggt gaacttttgc taattttagc agtccaaacc aaagttcaac
     3121 tctaacgcac tttaccatga agtattcaaa aacaattctt tatctctgtt ccatgacgct
     3181 gttaatatcc aattccggat gcaataaggc caacaaacca gctgctccgg aaccgttgag
     3241 catcaatgtt tctctttccg actctgaaaa agcagccgga ttgctaactc ccgaaatact
     3301 atggaagttc gggcgggtgg gcgagtatgc cctttctccg gacggtacaa tgcttgttta
     3361 cgcggttacc ttttacaatc tacaggaaaa caagggagtt accaacttgt atgttgtttc
     3421 tgctaccggt ggtgagcctc taaagttaac cgatgaaacc ggttctgagt ctgcaccaca
     3481 gtggagcagc gatggccaaa caatcctatt cctttcaacc cgtggtgggt cgaaccagct
     3541 atggcaggtg aggcctgatg gttcggagtt gaaacagatt tctggcattg atggcgatat
     3601 aaatggtttt tccttttcgc ccgataacac aaagctggtg tttgccgtcg atgttaagct
     3661 cgaccaacag gccgatgata tttaccccga tatgccgaag gctaaggtga ggattattga
     3721 caatctgatg taccgccatt ggaattactg ggaggacgga aggtatagcc acctttttgt
     3781 tgctgacttt gccgatggca aaatctcgaa tgcacgcgat ataaatgctg gagaaaagtg
     3841 ggacacccct atggctgttg attttgacat gaccgaagta cagtgga
//
LOCUS       DAOOQX010000108         3887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_27843_length_3887_cov_2.735125, whole genome shotgun sequence.
ACCESSION   DAOOQX010000108 DAOOQX010000000
VERSION     DAOOQX010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27843_length_3887_cov_2.735125"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PLX53_02620"
     CDS             <1..773
                     /locus_tag="PLX53_02620"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOY72001.1"
                     /translation="TTYHRNGNVDTRSVYRNGRLNGLTEVFAEDGKIKLRSEYLNDTL
                     NGVWQTYYESGELNYSGSKSKGKYDNVLLAYHKNGSLQRAARYHDGVLDGEVVSYYEN
                     GKPQVTSHFSMGKQTGEFVEYYPNGYVRERGMYKENQKEGMWMSFYPNGQLHGKGNFK
                     QGSLIGEWIFFHDNGNLKQRGSYCNGKACGLWQYFNPQGNLILCGVYVDDLQNDIWTT
                     FYPNGSVAGRILFVNNKPAGIRWVYQQNGSMEKRGGQL"
     gene            complement(862..2838)
                     /gene="gyrB"
                     /locus_tag="PLX53_02625"
     CDS             complement(862..2838)
                     /gene="gyrB"
                     /locus_tag="PLX53_02625"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOY72002.1"
                     /translation="MSSEGLKGEELISNGYSADSIQVLEGLEAVRMRPSMYIGDTSIR
                     GLHHLVYEVVDNSIDEALAGFCNRIDVTINEDGSITVVDNGRGIPVDYHEKEKKSALE
                     VVLTVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSIKLVAEVMRDGKLYRQEFSMGKP
                     VTSLEVVGESPSTGTIITFMPDSQIFTQSISFNFEIIASRLRELAFLNKGIRLTLTDK
                     RELVENGEAHYRHEEFYSVEGLKEFIKFLDGNRESLTEKIIYIESDKGDVPVEVAMQY
                     NTSFSENVHSYVNDINTVEGGTHLTGFRRALTRTLKKYADDSGMLSKLKFDINGDDFR
                     EGLTAVISVKVAEPQFEGQTKTKLGNPEVSLAVDQAVSEALANYLEENPRDARNIVQK
                     VILAAQARHAARKARELVQRKTVLSGSGLPGKLADCSDKDPLKTELFLVEGDSAGGTA
                     KQGRDRSFQAILPLRGKILNVEKAMQHKIFENEEIRNIFTALGVSIGTEEDSKALNLS
                     GLRYNKVIIMTDADVDGSHISTLIMTFFFRYMTDLINEGHLYIATPPLYLVKKGKEDR
                     YCWNEEERLKAVQELGKGRDSSVHVQRYKGLGEMNAEQLWITTMCPDTRFLRQVTIES
                     AAEADRIFSMLMGDEVPPRREFIEAHAKYANIDA"
     gene            3162..>3887
                     /locus_tag="PLX53_02630"
     CDS             3162..>3887
                     /locus_tag="PLX53_02630"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOY72003.1"
                     /translation="MTQYLELLEHILKNGVQKTDRTGTGTISVFGYQMRFDLSRGFPL
                     LTTKKLHLKSIIYELLWFLRGDTNIKYLNQNGVTIWDEWANREGELGPIYGYQWRSWP
                     ASDGRHIDQIAQVVGSITQNPDSRRHLVCSWNVGDIGKMALPPCHVLFQFYVAGGKLS
                     CQLYQRSADVFLGVPFNIASYALLTTMVARVTGLEPGEFIHTLGDAHIYLNHVEQVKL
                     QLTRDPRPLPTMHINPQVKSIFDF"
BASE COUNT         1032 a          930 c          891 g         1034 t
ORIGIN      
        1 ttaccaccta ccataggaat ggcaatgttg acacccggtc ggtgtaccgc aatggcaggc
       61 taaacggatt aaccgaggta tttgccgaag atggaaaaat aaagcttcgc tcggagtacc
      121 taaacgatac cctaaacgga gtgtggcaga cctactatga atcgggagag ctaaactata
      181 gcggaagcaa aagtaaaggt aagtacgaca atgtgctgct ggcttaccat aaaaatggat
      241 cgcttcagcg cgctgcacga taccacgatg gggttctgga cggcgaggta gtttcgtact
      301 atgaaaacgg taagcctcag gtaacctcgc acttctcaat gggaaagcaa acaggcgagt
      361 ttgttgagta ctaccccaac ggctatgtta gggaaagggg aatgtacaaa gagaaccaaa
      421 aggagggcat gtggatgagc ttttacccta acgggcagct tcatggaaag ggaaatttta
      481 aacagggtag ccttatcggc gaatggatat tttttcatga taacggaaac ctcaagcaac
      541 gtggaagcta ctgcaatggt aaggcttgtg gcttatggca gtacttcaac cctcaaggaa
      601 atttaatttt gtgcggagtt tacgtcgatg atttacaaaa tgacatttgg actacctttt
      661 acccaaatgg ttcggtagct ggcagaatac tttttgtaaa caacaaacct gctggaataa
      721 gatgggttta ccagcaaaat ggatcaatgg agaagagagg cggacagctt taggttcttc
      781 aactcaaccc cacaataaca aataagaggc tgaatgtcag cctcttattt gttatataac
      841 gtttatactc caacaggcat tttaggcatc aatgttggcg tacttggcat gtgcttcaat
      901 aaactcgcgc ctaggcggaa cctcatcgcc catcagcatg gagaatatcc tgtcggcctc
      961 agctgcactt tcgatggtaa cctggcggag gaatcgggta tcgggacaca tggtggtaat
     1021 ccaaagctgt tcggcattca tctcaccaag ccctttgtag cgctgaacgt gaacagagct
     1081 atcacgtccc ttcccgagct cctgaactgc ctttagcctc tcctcctcat tccagcagta
     1141 gcgatcctcc ttaccttttt taaccaggta aagcggggga gttgcaatgt agaggtgtcc
     1201 ctcgttaata agatcggtca tgtagcggaa gaagaaagtc atgataagtg ttgaaatgtg
     1261 gcttccgtcc acgtcggcat cggtcataat gattacttta ttgtaacgaa ggcccgacag
     1321 gtttaaggcc ttgctatcct cttctgtgcc aatacttaca ccgagtgcag taaaaatatt
     1381 ccgaatttcc tcgttctcaa atattttgtg ctgcatggcc ttttcgacgt tcaaaatttt
     1441 tcccctcaag ggcaggatgg cctggaagct cctgtcgcgt ccctgcttgg cagtgccgcc
     1501 agccgaatca ccctctacca gaaaaagttc tgttttcagg ggatccttat cggagcaatc
     1561 ggctagctta cctggaagtc ctgagccgga aagcacggtt tttctttgca ccagttccct
     1621 tgccttgcgg gcagcatgtc gtgcctgtgc ggcaagtatg accttttgaa caatgttgcg
     1681 ggcgtcgcgg gggttttctt ccaggtagtt tgccagcgct tcgcttactg cctgatcaac
     1741 agccaggcta acctcggggt tgccaagctt ggttttggtt tgaccctcaa actggggttc
     1801 ggccacctta acggatataa cagccgttaa tccctcccgg aagtcatcac cattgatgtc
     1861 gaatttcagc ttggaaagca tccctgaatc atcggcatac tttttcagcg tccgggtaag
     1921 cgctcttcga aaaccagtta ggtgtgtgcc cccctcaacg gtattgatat cgtttacgta
     1981 ggagtggaca ttttcggaaa acgaggtgtt gtactgcatg gccacctcaa ccggaacatc
     2041 ccctttatcg gactcgatgt aaatgatttt ctcggtcagg ctctcccggt taccatcgag
     2101 gaacttgata aactccttta acccctcaac cgagtaaaat tcctcgtggc ggtagtgggc
     2161 ttcgccgttt tcaaccaatt ctcgcttgtc agtgagtgtt aggcgaatgc ccttattgag
     2221 gaatgccaac tcgcgtaaac gcgaggcaat aatttcaaag ttgaagctaa tggattgggt
     2281 gaatatctgc gaatcgggca tgaaggtaat gatggtgcct gtgctgggtg actctccaac
     2341 cacctcgagc gaagtaacag gctttcccat tgaaaattcc tgacggtata gctttccatc
     2401 gcgcataacc tcggcaacaa gctttatgga gagggcgtta acacaagaaa ctccaacccc
     2461 atgcagccct cctgatactt tataactgcc cttgtcaaac tttccgccag catgtagaac
     2521 ggttagcacc acctcaagtg ccgatttctt ctccttttca tggtagtcaa ccggaattcc
     2581 cctcccatta tccactacgg tgattgatcc atcctcgttg atggtaacat caatcctatt
     2641 gcagaaaccg gctagcgctt cgtcaatgga attatcaact acctcgtaaa caaggtggtg
     2701 taatcccctg atgctggtgt cgccaatgta cattgatggt ctcatgcgaa cggcctccag
     2761 cccctcaaga acctgtatgc tatcggcgct ataaccgttg gaaatcaact cttctccctt
     2821 caaaccttcg ctgctcatac tacttataaa tatttggaat cagttgcaca ctttagtatt
     2881 gtaattaata agctaatttt taaaagccat caaaagtagt aaaaattttg gttaaacagc
     2941 acttcaaaaa ttactttcca ataatcatat agtgctatta tacagacaca tacgtatttg
     3001 ttgataaaat atttcaaaaa gtatgcccgg gttacagcag ctgtttgttg ttctgttttt
     3061 aaaagaagaa aagcttgaga gaaaaagcta acttcgcagc aaaacctgag gtagcccatt
     3121 tggcatagtt gaattttatt acctcaaata gaacttacac catgacccaa tatcttgaac
     3181 tactagaaca tatattgaag aacggagtgc agaagaccga caggacgggc acaggaacaa
     3241 tcagtgtttt tgggtaccag atgcgctttg atcttagcag agggtttcct ctgctcacca
     3301 caaaaaaact acacctgaag tcgatcattt acgagctgct gtggttttta agaggagata
     3361 ccaatattaa gtatctaaac caaaacggcg taaccatttg ggacgagtgg gccaaccgcg
     3421 aaggggagtt ggggccaata tacggctacc agtggcgctc gtggccagct tcagatggcc
     3481 ggcatattga ccagatagca caagttgtcg gctcaataac ccaaaatccc gattcgcgca
     3541 ggcatttggt ttgctcgtgg aatgtaggcg atataggtaa aatggcgctt cctccttgcc
     3601 atgtgctatt ccaattctac gttgctggcg gtaagctctc ctgccagctt taccagcgca
     3661 gcgccgatgt ttttcttgga gttcccttca acattgcttc gtatgctctc ctaaccacta
     3721 tggtagcccg tgttaccgga ttagaacccg gtgaatttat ccatacgctg ggtgatgccc
     3781 acatctacct caatcatgtt gaacaggtta agctgcagct gacgcgcgac ccgcgcccgt
     3841 tacccaccat gcacattaac ccgcaggtga aatctatttt cgatttc
//
LOCUS       DAOOQX010000109         3883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_27881_length_3883_cov_3.829676, whole genome shotgun sequence.
ACCESSION   DAOOQX010000109 DAOOQX010000000
VERSION     DAOOQX010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27881_length_3883_cov_3.829676"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="PLX53_02635"
     CDS             <1..1375
                     /locus_tag="PLX53_02635"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOY72004.1"
                     /translation="SFGVFAQNHNGYRIEIKIDGLKDTTLLLGYHFGDKKFVSDTARI
                     DSNGVAIFEGDSLLKGGIYLVILPDKTYFEVLLSNNQRFGVQTTAKNMVENLKFTGSK
                     ENEEFIHFQQFMMGVQAQSNELSAKMKDALTDSTRSNQLRKEMNELDEKVKAYWLEVE
                     NNNRGTFLASIIKAMRSVEVPEFTIPANTPAYDSLKWVHSYNFNRDHYFDNIDFSDSR
                     LLRTPILENKINIYFDRVLIPHPDSIAAAAVNLVEKSKANREMLQYILSYLINKFQTS
                     ERMGMDGVFVVLAEKYYLGGQAWWADQKLLDKISERVNALKPNLIGKVAPDLWLPDPN
                     GKYFRLSNINSKITVIFFWDPECSHCKKEMPKLKNVYDNYKKYGLEVYAVYTQGDQPK
                     WMESISKNDLTWINVWDPTFSSNFRNLYDIYSTPVIYVLDSGKKIIAKRISVETLESI
                     LKQELKL"
     gene            1659..3137
                     /locus_tag="PLX53_02640"
     CDS             1659..3137
                     /locus_tag="PLX53_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOY72005.1"
                     /translation="MESLLTNTYFWVVLAYFAVVLFISFFTKKLASRSSADFLVAGRN
                     LGLLVCSMVVAAEWLGGLSTVGVSEKAFDTLTLQPILYNFSTAIGMVILGFTLAKAYR
                     ERKVNTVSEMVESIFGKRARSISAIAFLIAFITLAYVELTTFATVLSPIFNINWVWAV
                     IIAATITTIYTYMGGMHALTIVAVFNLVVRYVGLGIALIIGLVAIGDFSNLTNRLVEI
                     GAPQNLYNPFSIGWNAALSLILGGIFGGMASQASIQPIFAAKNPITAKRAAILSAVFI
                     APFGIITGILGLIARSGLFFEVTPGFNPKMALPRLLMTPEFVHPVIGGIAIAAILAAI
                     LSTIGPVSFSIVTIATKDIYHGLINKKASDEKILQFAKRLVILVNLVTVPLALFMKGA
                     ILDAGYFSYAIRTIGAIVILLGIYKLKWISTLSVQLSFIGGTLAIIISIVAKKFGWFD
                     IDKTYASIIATVFFIIIGNIYTKYFTGKQKSSELNINESATK"
     gene            3118..>3883
                     /locus_tag="PLX53_02645"
     CDS             3118..>3883
                     /locus_tag="PLX53_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOY72006.1"
                     /translation="MNQLLNDIRVLELANVLAGPSVGMALAELGARVVKVENLLTLGD
                     VTRTWKLPSEDPDTNISGYFSCVNWGKKSLAIDLNKPEGLDIVYRLTEVSDIVLVSYK
                     PGDAEKLKVDYNTLKQYNKSLIYAHLTGYGLKNERAGYDAIIQAESGFTYMNGEQGGK
                     PVKMPVALMDILAGHQLKEAILLALLNRFRKGEGTYIEASLIKSGVAALANQATNWLV
                     GNSIPQRMGSDHPNIVPYGRIFYTRDEKPVVLAVGSD"
BASE COUNT         1087 a          794 c          870 g         1132 t
ORIGIN      
        1 ttcgtttggt gtatttgccc aaaaccacaa cgggtataga attgagatta aaattgatgg
       61 gctgaaggat accaccctgc ttttggggta ccactttggt gataagaaat ttgtgagcga
      121 tacagcacgt atagattcta acggtgtagc catttttgag ggtgatagct tgctaaaagg
      181 tggaatttac ctggtaatcc taccggacaa aacctacttt gaagttcttt taagcaacaa
      241 ccagcgtttt ggagttcaaa caacggctaa aaacatggtt gaaaatctga agtttacagg
      301 ctcaaaggag aatgaagaat ttattcattt tcaacaattt atgatgggtg ttcaggctca
      361 aagcaatgag ctatcggcca aaatgaagga tgcccttacc gattcaacca ggtcaaacca
      421 gcttcggaaa gaaatgaatg agctggacga gaaggtgaag gcctactggc tggaggtcga
      481 gaacaacaac aggggcacat ttctggcatc aattatcaaa gccatgagaa gtgttgaggt
      541 tcctgaattt acgattcctg ctaacacgcc tgcttacgat tccttaaagt gggttcacag
      601 ctataatttt aaccgcgatc actacttcga caatattgat ttcagcgatt cacggcttct
      661 tcgcacccca attctggaga ataagataaa catttacttc gatagggtgc ttatacccca
      721 tcccgatagc attgcagcgg cagcagttaa cctggttgaa aaatcgaagg caaacaggga
      781 gatgttgcag tacattcttt catacctaat taacaagttt caaacctcag aaaggatggg
      841 catggatggc gtgtttgttg tattagccga aaagtactat cttggaggcc aagcttggtg
      901 ggccgaccag aaacttctcg ataaaatttc cgaaagggta aatgcactaa agcccaacct
      961 tattggtaaa gttgcccccg acttatggct acccgatccg aatggcaagt actttaggct
     1021 tagcaatatt aattctaaaa ttactgtaat tttcttttgg gatcctgagt gcagccattg
     1081 taaaaaggag atgccgaagc tcaaaaacgt ttacgacaac tacaaaaagt atggccttga
     1141 ggtgtatgcc gtttacaccc agggcgacca gcccaaatgg atggaaagca tttctaagaa
     1201 cgacttaacc tggattaatg tttgggaccc tacattcagc tctaatttcc gaaacctcta
     1261 cgacatttac tccacgcctg ttatttacgt cctcgattcc ggtaaaaaaa ttatagccaa
     1321 acggattagc gttgaaaccc tcgaatcgat tctaaagcag gaactcaagc tgtagtgatt
     1381 ttttcctcag taaaatttca gcttaagggt tgaaaaatcc tgaacattcc cgtaaatgat
     1441 tttgaccaag attagcagac ccttcactgt tgtgtctggt tattggtcat ggttgtcacg
     1501 gtatatttgt tgataaatcc gaacagaacg gattgtttta ctttcatatt gttagccaat
     1561 atccgaaatt tataaaaatt ataagctgtt ttaggctaga ggatggaatt tttactatct
     1621 tggccgcaaa actctaatca acttaacatt taagatttat ggagagctta ctgaccaata
     1681 cctacttttg ggtagtgtta gcctattttg ccgtggtttt gtttatctcg ttttttacaa
     1741 aaaagcttgc tagccgatcg tcagccgact tcttagttgc cggtcgaaac ctggggcttc
     1801 tggtgtgttc aatggtggta gccgccgaat ggctgggtgg gttgagcaca gttggcgtta
     1861 gcgaaaaggc attcgacacc cttaccctgc aacctattct ctataatttt tccacagcta
     1921 ttggaatggt gatactcggc tttactttag ctaaagctta ccgcgaacgg aaggtgaata
     1981 cggtgagcga aatggtagag agcatttttg gaaaacgtgc ccgaagcata tcggctattg
     2041 cctttctaat tgcttttata acgcttgcct acgtggagct aaccactttt gcaactgttc
     2101 tttcgccaat ttttaacata aattgggttt gggccgtcat aattgcagct actataacca
     2161 caatttacac ttacatgggc ggaatgcacg ccctcactat tgttgctgtg ttcaaccttg
     2221 ttgttcggta tgttgggttg ggaattgccc taattattgg gcttgttgca attggcgatt
     2281 tttcaaattt aaccaacagg cttgttgaga ttggcgctcc tcaaaatttg tacaatcctt
     2341 ttagcatagg atggaatgct gcgttgagcc ttatactggg tggaattttt ggtggaatgg
     2401 cttcccaagc cagcattcaa cctatttttg cagctaaaaa tcctattacc gcaaaaaggg
     2461 ctgccatttt atcggcagtt tttatagctc cttttggaat tatcacagga attcttggtc
     2521 ttattgcccg aagtggtttg ttttttgaag ttacccccgg gtttaacccc aaaatggccc
     2581 ttccacgctt gctaatgacc cccgaatttg ttcatcccgt gattggggga attgcaattg
     2641 cggcaattct tgcggcaatt ttatctacca tcgggccggt gagcttttca attgttacca
     2701 ttgctactaa ggatatatac catgggttga taaacaaaaa ggcatccgat gagaaaatcc
     2761 tccagtttgc taaaaggctc gtaatactgg taaatctggt aaccgttcct ttagccctgt
     2821 ttatgaaggg cgcaattctt gatgcagggt atttttccta tgccattcga acaattggtg
     2881 caatagtgat acttttgggt atttacaaat tgaagtggat ttctaccctg tctgttcagc
     2941 tttcctttat tggcggcacg ttggccatca ttattagcat tgtagccaaa aagtttggtt
     3001 ggtttgacat tgataaaacc tatgccagca tcattgcaac agtatttttc attattatag
     3061 gcaacatcta cacgaagtac tttacaggta aacaaaaatc atcggagtta aatatcaatg
     3121 aatcagctac taaatgacat aagggttctt gagctggcca acgtgctggc cgggccaagt
     3181 gttggaatgg cgttggctga gctgggcgcc agagtggtaa aggttgagaa cttacttacc
     3241 ttgggtgatg taacccgaac ctggaaatta ccatccgaag atcccgatac aaatatttcg
     3301 ggatacttca gctgtgttaa ctgggggaaa aagtcgttgg ccatcgatct caacaaaccc
     3361 gaggggcttg acattgttta caggctaacc gaggtttcgg acatcgtgct ggttagctat
     3421 aaacccggcg atgccgaaaa actgaaggtg gactacaata cccttaaaca atacaacaaa
     3481 tcgctgatat acgcccatct aaccgggtat ggattaaaga atgaacgcgc tggatacgac
     3541 gcaatcattc aggccgaaag tggctttacc tacatgaatg gtgaacaagg agggaaacct
     3601 gtgaaaatgc ccgttgcact gatggatata cttgccggac atcagctaaa agaggcaatt
     3661 ttgctggctc tgctgaatcg ctttagaaaa ggtgaaggga cttatattga ggcgagcctt
     3721 attaaatcag gagttgccgc tttggcaaac caagccacca actggttggt gggaaattct
     3781 atcccccagc gcatgggttc cgatcatccc aacattgtgc cctatggaag aattttttat
     3841 acccgcgacg agaaacctgt agtgctggct gttggcagcg ata
//
LOCUS       DAOOQX010000110         3871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_28013_length_3871_cov_2.360325, whole genome shotgun sequence.
ACCESSION   DAOOQX010000110 DAOOQX010000000
VERSION     DAOOQX010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28013_length_3871_cov_2.360325"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PLX53_02650"
     CDS             <1..938
                     /locus_tag="PLX53_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HOY72007.1"
                     /translation="RSLIKKWETATEKLKTVSFPGQLASELKRTSAILRDMLNGTFNS
                     IHVNDEIVFNDIHEYIETIAPEKEKIVKLYTGNVPIFDQFGIEKQIKSLFGKTVSIKS
                     GAYLIIEHTEALHVIDVNSGNRSKSGNDQETNALDVNLAAADEIVRQLRLRDMGGIIV
                     VDFIDMHQNENRNMLFERMKELMSKDRAKHNILPLSKFGLMQITRQRVRPEMHINTTE
                     KCPTCKGTGEVSPTVLLDEQIENQLSTLVTSHKLKSITLKLHPYIAAFLNKGFVSKRM
                     RWSFRYHCRINVTPMSSYHFLEYHFFDSKDTEIGI"
     gene            complement(1057..2088)
                     /gene="recA"
                     /locus_tag="PLX53_02655"
     CDS             complement(1057..2088)
                     /gene="recA"
                     /locus_tag="PLX53_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005636551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOY72008.1"
                     /translation="MEKVEKEKEKKERKEISQDKLKALQSTLDKIEKDFGKGSIMKMG
                     DKAVADVASISSGSIALDIALGIGGYPRGRVVEIFGPESSGKTTLAIHAIAEAQKLGG
                     IAAIIDAEHAFDRSYAEKLGVDVETLLISQPDNGEQALEIADNLIRSGAIDIIVIDSV
                     AALTPKAEIEGEMGDSKMGLQARLMSQALRKLTGTISKTNTCCIFINQLREKIGVMFG
                     NPETTTGGNALKFYASVRVDIRKLNQLKDGEESTGNRVRVKIVKNKLAPPFKKAEFDI
                     IFGEGISRTGEIVDLGVECNIIKKSGSWFSYGETKLGQGRDSVRQLLQDNPELASEIE
                     AKIRETLKG"
     gene            complement(2101..2577)
                     /gene="bcp"
                     /locus_tag="PLX53_02660"
     CDS             complement(2101..2577)
                     /gene="bcp"
                     /locus_tag="PLX53_02660"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOY72009.1"
                     /translation="MKTTFLKPGDTAPDFTAVDQDGVEFSLSEYQGRAVVLYFYPKDN
                     TTGCTEEACSLRDGYTELKQLGLDVIGVSPDSTRSHRSFIQKHSLPFRLAADTDHRIA
                     ELYGVWAEKSMYGKTYMGIVRTTFIINGDRKITHVFDKVNTKAHAMQILEELKKTL"
     gene            complement(2820..>3871)
                     /locus_tag="PLX53_02665"
     CDS             complement(2820..>3871)
                     /locus_tag="PLX53_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HOY72010.1"
                     /translation="SSFQTEFWQVGITAEYGFGHLFGLVKRFKPFIAVGVSTMFFTPK
                     GNVRYGPTGDFYYAWSDGSLRDFPENGPNAYLAQIIKLDNSYETDLSSADLYRIGSYS
                     QNTFTIPLDLGFDFYLSDRVNFRVGATVNYAFSDLLDNYSSKTASTMGYPNKNSYNDI
                     FAFTYFSMNLDLFSDPKTMLVERVFAELSGTFDYGVFFADQDGDNVLDRFDACPDTPP
                     GVAVDSLGCPPDYDNDGVFDYMDDESSTPEGAIVNERGVQLSADVLSDMFSKNSMAVP
                     RSEVKVIPLANVWTRNATFTPGAIPDKFRIVDADADGYISFQELLKAINDFFDEKNTF
                     TPDEIYELNEYFFSQ"
BASE COUNT         1049 a          908 c          861 g         1053 t
ORIGIN      
        1 ttcgcagcct tatcaagaag tgggaaaccg caaccgaaaa gttaaaaaca gtatccttcc
       61 ccggccagct ggccagcgag ctaaagcgaa cctcggccat ccttcgggat atgctgaacg
      121 gaacgttcaa cagcattcat gtgaacgacg agattgtgtt caacgatata catgagtata
      181 ttgaaaccat tgcccctgaa aaggagaaaa ttgtcaaact ttacacggga aatgttccca
      241 tattcgacca gtttgggatt gaaaagcaga taaaatcgct attcggcaaa actgtctcaa
      301 taaaaagcgg ggcatacctc atcatagagc acaccgaggc tttacatgta attgatgtta
      361 acagcggaaa tcgttcaaaa tctggtaacg atcaggaaac caacgcactg gatgtaaacc
      421 tggcagcggc cgatgagatt gtccgtcagc tcaggcttcg cgatatgggc ggcatcattg
      481 tggttgattt tatagatatg catcagaacg agaaccgcaa catgctgttc gagcggatga
      541 aggagctgat gtcaaaagac agggctaagc ataacatttt accgttgagc aagtttgggc
      601 taatgcagat aacccgccag cgagttcgtc ccgaaatgca tatcaacact accgagaaat
      661 gccccacgtg caaggggaca ggagaggtta gccccactgt tctgctcgac gagcagatcg
      721 aaaatcaact ttccacgctg gttaccagcc acaagttaaa aagtattacc cttaagctgc
      781 acccctacat tgcagctttc ctaaacaagg gttttgtttc aaagcgtatg cggtggtcgt
      841 ttaggtacca ctgcaggata aatgtaactc ccatgtcgtc ctaccacttt ctggagtacc
      901 atttttttga ctccaaggat accgagatag gtatatagcc aacaaaaaaa ccccgctgcc
      961 aggcagcggg gtttaacttt atgcagtaac aaagttctgc atcaatagga gtggtaaaaa
     1021 tatcagattt ttgttgccaa acgctctacc tgaccgctaa ccctttaacg tttcgcgaat
     1081 tttggcctca atttcgctgg ccaactcggg attgtcctgt aggagctggc gaacgctatc
     1141 gcgaccttgt cccagctttg tttcaccgta gctgaaccac gatccacttt ttttaataat
     1201 gttacactcc acaccaagat caacaatttc accggttcgt gagattcctt ctccaaaaat
     1261 gatgtcgaac tcggctttct tgaacggagg agccagctta ttctttacaa ttttcaccct
     1321 cacccggtta ccggttgatt cctccccatc cttaagctgg ttgagcttac gaatatcaac
     1381 cctaaccgaa gcgtaaaact taagggcatt ccccccggta gtggtttcgg gatttccgaa
     1441 cataacgccg attttttcac gcagctggtt gataaaaatg cagcaggtgt tagttttgct
     1501 aattgttccg gttagctttc gaagggcctg tgacataagc cttgcctgaa ggcccatttt
     1561 gctatcaccc atttccccct caatttctgc cttaggggta agggctgcca ccgagtcgat
     1621 aacaattata tcaattgccc ctgagcggat aagattatcg gctatttcaa gcgcctgttc
     1681 tccgttgtcg ggttgcgaaa tcaacagtgt ttccacatca actcccagtt tctcggcgta
     1741 tgagcggtca aaagcatgct cagcatcaat tatggcggca atgccaccaa gtttttgagc
     1801 ttcggcaatg gcgtgaatgg ccagggttgt ttttcccgac gattcgggtc cgaatatttc
     1861 aaccaccctg cctctagggt atcctccaat gccaagcgct atatccagag caattgaccc
     1921 cgaggatatt gaggcaacat cagcaactgc cttatcaccc attttcatga tggacccctt
     1981 cccaaaatct ttttcaattt tgtcgagggt tgactgaaga gccttcagct tatcctgact
     2041 tatctccttg cgctctttct tttctttttc tttctctacc ttttccatgt ggttggtatg
     2101 ttaaagcgtt tttttcaact cctctaaaat ttgcatagca tgggctttag tattgacctt
     2161 gtcaaaaaca tgggtaattt tccggtcgcc attaattatg aaggtggtgc gaacaattcc
     2221 catgtaagtt ttgccataca ttgatttctc agcccaaacc ccatacaact cagcaatccg
     2281 atggtcggtg tcagcagcca acctgaaggg aaggctgtgc ttctggataa atgacctatg
     2341 cgacctggtg ctgtcggggc ttaccccgat aacgtcaagc cccagctgtt taagttccgt
     2401 gtacccatcc cttaggctgc aagcctcttc ggtgcagcca gttgtgttat ctttgggata
     2461 aaagtaaagc actactgctc tgccctggta ctcggaaagg ctgaattcaa cgccatcctg
     2521 atccactgct gtaaagtcag gagctgtatc gccaggtttt agaaaggtcg ttttcatcac
     2581 tttgattttg taaagttatg aagaatatac aaaatagctc tcgggttgtt caaaaaatct
     2641 accaaagact tatcaacaga aaataccgta accttcattt gcttgttcct gacaaaattt
     2701 atcaccctga agttaaaaaa aaccctacag gaattctgta gggcaggtag taaaagggtt
     2761 ttaggttagg ttatataggg ttgtttgggt ttataggtta attggtttta ggtttttgct
     2821 tactgtgaga aaaagtattc gttgagctca taaatttcat cgggggtaaa ggtgtttttc
     2881 tcatcaaaga agtcgttaat tgccttgagc agctcctgga aagagatata tccatcagca
     2941 tctgcatcaa cgatgcggaa tttatcggga attgcaccag gggtaaaggt tgcgtttcgt
     3001 gtccagacat ttgcgagggg aataacctta acctcacttc tgggcactgc catggagttt
     3061 ttgctaaaca tatcggaaag cacatcagcc gagagctgca ctcccctctc gttgacaatt
     3121 gctccctcag gggtactgga ctcatcgtcc atatagtcga atactccgtc gttgtcataa
     3181 tcgggtgggc aaccaagcga atccacggcc actcctggag gtgtatcggg acatgcatca
     3241 aacctatcca acacattatc gccatcctga tcggcaaaga aaaccccgta gtcaaatgta
     3301 ccactaagtt cagcaaacac cctctcgacc agcatggtct ttgggtcgga gaaaaggtcg
     3361 aggttcatgc tgaaataggt gaatgcgaat atatcgttgt agctgttttt attggggtag
     3421 cccatggtgc tggcagtttt gctgctatag ttgtccagca aatctgaaaa ggcatagtta
     3481 acggtagcac ctaccctaaa gttaaccctg tcggacaggt aaaaatcgaa acccaggtca
     3541 agtggaattg taaaggtatt ctggctgtag gaaccaattc tgtacaaatc ggcgctggaa
     3601 aggtctgttt cgtagctatt atcgagtttg attatttgag ctaagtaggc attaggacca
     3661 ttttcgggga aatcacgaag cgaaccgtcg gaccaagcat agtagaagtc accggtaggc
     3721 ccatacctga catttccctt aggcgtaaaa aacatagttg aaacgccaac tgcgataaat
     3781 ggtttgaagc gcttaaccag cccaaataga tgaccgaaac catactcagc agttatgcca
     3841 acctgccaga actcggtttg gaatgaggag t
//
LOCUS       DAOOQX010000111         3854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_28179_length_3854_cov_3.028955, whole genome shotgun sequence.
ACCESSION   DAOOQX010000111 DAOOQX010000000
VERSION     DAOOQX010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28179_length_3854_cov_3.028955"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLX53_02670"
     CDS             <1..184
                     /locus_tag="PLX53_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72011.1"
                     /translation="GTTRPAFGVAQTVPLGNSHAMFRPRPICGSGFYAFFLIPATFNV
                     SRLSGHCGEYLFPRWG"
     gene            complement(466..645)
                     /locus_tag="PLX53_02675"
     CDS             complement(466..645)
                     /locus_tag="PLX53_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72012.1"
                     /translation="MVSVVGRMEKMEKRLAIVKTELFDGKGILCSTGQITYFTYSAEV
                     AKQQFGYPGLAEFLS"
     gene            complement(673..936)
                     /locus_tag="PLX53_02680"
     CDS             complement(673..936)
                     /locus_tag="PLX53_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72013.1"
                     /translation="MRNPYQNQPSHGCFCCSSHNPIGLKLEFWFDDERGTVGTQWLPS
                     ENFQGYHDVLHGGIQSTLMDEVASWCIYILAGTAGVTSKMEVA"
     gene            1068..1670
                     /locus_tag="PLX53_02685"
     CDS             1068..1670
                     /locus_tag="PLX53_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOY72014.1"
                     /translation="MHGNATKMKLMETARELFSAKGVDRVGVREIAAKAGVNLSLMNY
                     YFESKENLLEQIFEFSVRENGHKLRQILSSGLPLDEKIRQYVYMYIDILIDDPLLVPF
                     VLSTIHRKADKSPYLKSIHTLYSTESFSKQLKEEADKGHIRPIDTEQFFVSMISLIIF
                     PFAVEPLVKYRMSLNNDQMNIFLAARKEHVFDMLMAVLKA"
     gene            complement(1795..3162)
                     /gene="lpdA"
                     /locus_tag="PLX53_02690"
     CDS             complement(1795..3162)
                     /gene="lpdA"
                     /locus_tag="PLX53_02690"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOY72015.1"
                     /translation="MDYDVVVIGAGPAGYVAAIRAGQVGLKTAIVEKKLIGGMCLNWG
                     CIPSKAIIESGRLYRRIKDDARFFGIDGIDEQNITFSWEQARKRALQVAKQLTSGVEL
                     MLKKNGVEIINGEARITSENSVTVANRNITASNIIIATGSHHLPLKAKLPSEKVVELE
                     QFYSLKELPEHIAVVGEGSIAIELAQFFNLIDRNVTILVPGERMMPLADEYLASWMLS
                     KLKRDGIRVIFNANDMADSSHFEGNQLVVGKDKFPCDIIINSKLRQGIIPPSDIPLET
                     ANGFIATNDFFQTSFPNIFAVGDVNGEMMFANAASTQGFHTINFIKGIKEPFDKSRYP
                     LNMYSSPEVAQIGKIESQLKADGVNYRVSELPLSANGKALSEGNTEGFVRILSEVKYG
                     QVLGVQIIASNATDMISEAAAFMEAEATIYDVAKTVHAHPTISEVFMEAGFVAVDSAK
                     EENRQ"
     gene            complement(3179..>3854)
                     /locus_tag="PLX53_02695"
     CDS             complement(3179..>3854)
                     /locus_tag="PLX53_02695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOY72016.1"
                     /translation="TQIRKTIARNMIQSKQNAAHMSVFDEVEVSELVRIRAKYKDAFA
                     EKGVKLSYLPFILKALAVALSHHKTLNSQLDLENNRIIYKKYFNIGIAVDTEEGLVVP
                     VIRGVDKLSVFEIAERVNEIAEKARNRKLTLDDMKDGTFTVTNYGSIGGQFAVPVINY
                     PQVGILGIGRLVEKPVISHGELAKGMVLPLSLSVDHRIVDGGEVARFLNRLIAYLADP
                     VSLIME"
BASE COUNT         1004 a          953 c          829 g         1068 t
ORIGIN      
        1 cggcacaact cgccccgcct tcggcgtggc tcaaacagtg ccgcttggga attcccacgc
       61 tatgttccgc ccccgaccca tttgcgggtc tggcttttat gccttttttc taataccagc
      121 aacgttcaac gtttcccgcc tttccgggca ctgcggagaa taccttttcc cccgttgggg
      181 ctgaagaaaa gggtaaaggt taatacaagg ataaaggtta aaggataaag gataaaaaaa
      241 ttcaagcaag attcaagaac ctgtccgcct actggcggaa cttgtccggc tcctgacgga
      301 ttcacccttc gacaggctca gggttcaaac ctgtccgcct actggcggat ttaagaacct
      361 gtccggcttt tgacggaagg ttgagtggtt ctgacggtta tattgttcaa tttctattta
      421 tcaactttca acctctaccc tgtttcctgt atcccttccc tacacctagc ttagaaattc
      481 agccagtcca ggataaccaa attgctgctt agctacttcg gctgaatagg taaagtaggt
      541 tatttgaccg gttgagcaaa gaatgccttt accatcgaat agctctgttt taactattgc
      601 caaacgtttc tccatttttt ccatcctacc cacaacgcta accatgccat tcgagatttg
      661 aactggtttt ttttaggcaa cctccatctt tgaagttact cctgcagtcc cggctagtat
      721 gtatatgcac cagctggcaa cctcgtccat cagggttgac tggattccgc catgaagcac
      781 atcatggtat ccctgaaaat tctcagaagg aagccactgt gtccctacag ttccccgttc
      841 atcgtcaaac caaaattcca acttcaaccc aatggggttg tgggacgagc agcagaagca
      901 tccatgacta ggctggttct ggtatggatt tctcaacttt ataaccatgt ttatcgagta
      961 gtattattgc acacaatttt agcattattt cacaaaactt taagcctagt agctctttgt
     1021 tttttagcga aagagttaaa tttggtaaac ctataaatct ggactctatg catgggaatg
     1081 ccacaaaaat gaagctgatg gaaaccgccc gcgagctatt tagcgcaaag ggcgtagata
     1141 gagtcggagt tcgtgaaatt gcagctaaag caggggttaa cctttcactc atgaactact
     1201 atttcgagag caaggagaac ctgctagaac aaattttcga attttcggtt agggaaaatg
     1261 ggcataagct taggcagatt ctgagctctg gccttccgct ggatgagaaa ataaggcaat
     1321 acgtttacat gtatattgac attttaattg atgaccctct tctggttccc tttgttctgt
     1381 caaccatcca cagaaaggca gataaatcgc cctacctaaa atccattcac accctataca
     1441 gcaccgaaag tttctccaag cagctaaagg aagaggcaga taagggacac atcagaccta
     1501 ttgacaccga acaattcttt gtgagcatga tctccctaat tattttccct tttgcagttg
     1561 agcccttggt gaagtatagg atgagcctta acaacgatca aatgaatatc tttctggctg
     1621 cccgcaagga gcatgttttc gacatgctga tggcagtgtt aaaagcatag gttgatgaga
     1681 gccggcgcta gggtaatcgt gcaaaacaaa gtggaagcat ggggcaatat agctgaaaag
     1741 gccacggctt acctagtata ggctccgtga cctcagcagt tcatcctgaa aaggttactg
     1801 tctgttttcc tccttagcgc tatcaaccgc tacaaatcct gcttccataa aaacctccga
     1861 aatggtggga tgggcatgaa cggtcttggc cacgtcgtag atggtagcct cggcctccat
     1921 gaatgcagca gcttccgata tcatatcagt tgcgttgctt gctataattt gcactcctaa
     1981 aacctgccca tattttactt cggataggat tcttacaaac ccttccgtat tgccctccga
     2041 aagggctttc ccattagcag aaagtggaag ttcgctcacc ctgtagttca ccccatcggc
     2101 ttttagctgg gattcaatct tgccaatctg cgcaacttcg ggtgaagaat acatgttaag
     2161 cgggtacctg gacttatcaa aaggctcctt tattcctttg ataaagttaa tagtgtggaa
     2221 gccttgagtt gacgctgcat tggcaaacat catttcacca ttcacatcgc ctaccgcaaa
     2281 aatgttggga aaactggttt gaaagaagtc attggtagca atgaaaccat ttgccgtttc
     2341 aaggggaatg tccgagggcg gaataatgcc ctgccgcagc ttagagttga ttataatatc
     2401 acaggggaat ttatctttcc caacaaccag ctggttccct tcaaaatggc tactatcggc
     2461 catatcgttg gcattaaaaa ttaccctgat tccatccctt ttcagcttac tgagcatcca
     2521 gctggcaagg tactcatcgg caagcggcat catacgttcg ccgggaacga gaatggtaac
     2581 gttgcggtca atgaggttga agaattgagc cagctcaatg gcaatggatc cttccccaac
     2641 cacagctata tgctccggga gctccttaag actataaaat tgctccagct caaccacctt
     2701 ttctgatggg agcttggcct ttaaaggcag atggtgcgag ccagttgcaa taataatgtt
     2761 gcttgctgta atgtttctat tcgcaacggt aacagaattt tcggaagtaa tccgagcctc
     2821 cccgttgatg atttcaaccc cattcttttt cagcattaat tcaaccccgg aggtgagctg
     2881 tttggcgact tgcagggctc ttttgcgcgc ttgttcccag ctgaaagtga tgttttgctc
     2941 gtcaatacca tcaatgccaa aaaatcgggc atcgtcttta attcgcctgt acagcctgcc
     3001 gctttcgatg attgccttag agggaatgca gccccagtta aggcacattc ctccaatcag
     3061 cttcttttcc acaatggcgg tttttaaacc cacctgtccg gcacgtatgg cagccacata
     3121 tcctgccggg ccagctccaa taactactac atcgtaatcc atatcaatat tttaaagttt
     3181 actccatgat tagcgaaaca ggatcggcaa gatacgcaat aagcctgttg aggaatcgcg
     3241 ccacctctcc accatccaca attctgtggt caaccgaaag tgaaagtggg agtaccattc
     3301 ccttggcgag ctcgccatga cttataaccg gcttctccac cagccttcct atacccagta
     3361 ttcctacctg cgggtagtta atgacgggaa cggcaaattg ccctccaatg gaaccatagt
     3421 tggtaactgt aaaagttcca tccttcatat cgtcgagggt tagcttacgg ttacgggctt
     3481 tctcggcaat ttcgtttaca cgctcggcaa tttcaaaaac gctgagctta tcaacacctc
     3541 tgattacagg aacaaccagt ccttcctcgg tatcaaccgc tataccaatg ttgaagtact
     3601 tcttgtagat tatccggttg ttctccaaat cgagctggga gtttaacgtt ttgtggtggc
     3661 taagagccac ggccagggct ttcaggataa atggcaggta gctaagctta acaccctttt
     3721 cggcaaatgc atccttatac tttgcccgaa tgcgaaccaa ctcgctgact tcgacctcgt
     3781 cgaacacgct catgtgtgcg gcattctgct tcgactgaat catgttccgg gcaatggttt
     3841 tccttatctg ggtg
//
LOCUS       DAOOQX010000112         3847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_28249_length_3847_cov_3.456224, whole genome shotgun sequence.
ACCESSION   DAOOQX010000112 DAOOQX010000000
VERSION     DAOOQX010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28249_length_3847_cov_3.456224"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..434
                     /locus_tag="PLX53_02700"
     CDS             36..434
                     /locus_tag="PLX53_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY72017.1"
                     /translation="MLKLELESDKPVVLADEVHITNVVNNLLDNAVKYKKGNPEITIF
                     TKNSTDGIVVTFQDNGIGISRENLRKIFDQFYRVPSGNIHNVKGFGLGLSYVKKIVEA
                     HGGRIWVESTPGEGSLFSIFLPWNGPSEKK"
     gene            462..1154
                     /locus_tag="PLX53_02705"
     CDS             462..1154
                     /locus_tag="PLX53_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY72018.1"
                     /translation="MEKRTIRVLLAEDDENLGFLLKEYLQAKEYDVDLYKDGEKAYRG
                     FQNNYYDICILDVMMPIKDGFTLAKEIRMANPAMPILFLTAKSLKEDVIEGFSIGADD
                     YMTKPFSIEELLMRIEAILRRTRKDSPDSEQSSFQIGRYLFDATKQTLQLDDEVRKLT
                     TKESELLKYLCINKNSLLDRNFALKTIWVDDSYFNARSMDVYITKLRKYLADDPTIEI
                     INVRGKGFKLIF"
     gene            1278..1658
                     /locus_tag="PLX53_02710"
     CDS             1278..1658
                     /locus_tag="PLX53_02710"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOY72019.1"
                     /translation="MDKIIIEPTLETPRVVLDRDSSIIEFSGNSLPEDVNTFYAPILE
                     WIDNYVDNPNQQTEVSMNFDYYNTSSSKMILRILERFRDIHRKGYPVVVNWLYMEDDE
                     DMVEAGEDFADHLKIPFKFIPIQH"
     gene            1682..3154
                     /locus_tag="PLX53_02715"
     CDS             1682..3154
                     /locus_tag="PLX53_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOY72020.1"
                     /translation="MATPSKLKVFSNRLFGRFKSTEEVESNRKALEVEYYEWVEFAQS
                     DDYKRFEELKSWEATKEYLTVKKEVEAIKYSESPEQREEQELKKLSRSHSIRNFLKIA
                     KSETPDFYQKMEHSQLVKELKELQQFIATPDYQAKKNQYRKDNSAEYQKELRLKELLS
                     NPDIKKYLKLEKENDLKEYFQTENSEQLKRYNELSEKVSTEDFKNRKAYLLSKNKFEQ
                     TDAYKNLQEFKQLESSEKIKWFFRLNRSNKFDDLKAWNLAFYDDFDSKNIDPHKWLTK
                     FFWGEALVGKPYSFTTDNQNYTEKGNIEIGNSTLKIITRKEASEGLAWDKKFGFTPKA
                     FAYTSGIINTGHTFRLKEGRIEAKVRMSSVQGISHAFYLVGNEMLPEVDVFLKNNAKP
                     SGISAAYFWPNGKSKVAKSVSSIGGLKLGENFYIFGVEWDSNNIVWTINGIPYKVEPN
                     RHPGISYYMVFTSAVKGSVDDSLLPATLELDWVKCWEKKE"
     gene            complement(3178..>3847)
                     /locus_tag="PLX53_02720"
     CDS             complement(3178..>3847)
                     /locus_tag="PLX53_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72021.1"
                     /translation="WIALLENSRQIIIESLLRQKAKTVRILGSGNLLDVPVDEILQHG
                     STLLLTDIIHPPQVVNRFYGASSVSFETVDLTGGLVDFIAQRHARKLTFFELENFIRN
                     ITLPTFDEDLVVSLNLLSQLNDIPIAYLRHKGKLSNEQLHELSEQIQQRHVDQLPVGR
                     TLLVSDYEEEYYDEDGNLVGANPTVFVRIPGCTIREWSWQFDTQMTYHEDFKTVLRVT
                     ARQW"
BASE COUNT         1188 a          883 c          855 g          921 t
ORIGIN      
        1 acaactttgc cctctagctt caatccgaaa acggggtgct taagcttgag ctcgaatccg
       61 ataaaccggt ggtgttagcc gatgaggtgc atataaccaa cgtggtaaac aacctcctcg
      121 acaatgccgt gaagtacaaa aagggcaatc ccgaaataac tatttttacc aagaatagca
      181 ctgacgggat agtggtaaca tttcaggaca acggaattgg cattagccgg gaaaacctgc
      241 gaaaaatatt cgatcagttt taccgcgtac ctagtggtaa cattcacaac gtgaagggct
      301 ttggacttgg actgagctat gtgaaaaaaa ttgttgaggc acatgggggt cgaatttggg
      361 ttgaaagcac ccctggcgag ggtagcctgt tctcaatatt tttgccatgg aatggccctt
      421 cagaaaaaaa gtaaaaccat aaaccaaaat ccattacaat catggaaaaa agaacaattc
      481 gagtgctgct ggccgaagat gatgaaaacc tggggtttct gcttaaagag tacctccagg
      541 ccaaggaata cgatgtagat ctttacaagg atggcgaaaa agcctaccgg ggttttcaga
      601 ataactacta cgatatctgc atacttgacg taatgatgcc cattaaggat ggatttacac
      661 tggccaaaga aattaggatg gctaatcccg ccatgcccat ccttttcctt acggccaaat
      721 cgctcaaaga agatgtaatt gaaggattct caataggtgc cgacgactac atgaccaagc
      781 ccttcagcat tgaagagctg ctgatgcgta tcgaagccat actccgccgc acccgtaagg
      841 attcgcccga cagcgagcaa agctccttcc agattggccg ctacctattc gacgctacca
      901 agcaaaccct acagctcgac gacgaggtac gcaagcttac caccaaggag agcgagctgc
      961 taaaatacct ttgcatcaac aaaaactcgc tgctcgaccg taattttgcg ctgaaaacca
     1021 tctgggtgga cgacagctac tttaacgccc gcagcatgga tgtgtacatc acaaaactcc
     1081 gaaaatacct ggccgatgat ccaaccatcg aaattatcaa cgttagagga aaggggttca
     1141 agcttatttt ttagcctggg tactgcaacc aaaaatctat ttacgcatat atttgtagcg
     1201 taaaatgctt ttttggctgt tttttgcatt gtaaaaaaaa tattcttacc tttaggtagc
     1261 tgaaattgaa tgccggtatg gataaaatta ttattgaacc cacccttgaa acacctcgtg
     1321 ttgtgctgga cagagacagc tccataattg agttttcagg aaattctcta ccggaggatg
     1381 ttaacacttt ttacgctcct attcttgaat ggatcgacaa ctacgttgac aatcccaacc
     1441 agcaaaccga ggtcagcatg aactttgact actacaacac ctcctcgtca aaaatgatac
     1501 tccgaatact cgaaagattt cgcgacatcc accgtaaagg ctatcccgtg gttgtcaact
     1561 ggctttacat ggaggatgat gaggatatgg tggaagccgg tgaggatttt gccgaccacc
     1621 tgaaaatccc cttcaagttt atcccaattc agcactagca gctaaaccaa aaaccgactc
     1681 aatggctacc ccgtcaaaat taaaagtatt ctccaaccgc ctttttggca ggttcaagag
     1741 cacagaagag gttgaatcga atcgcaaggc gcttgaggtc gaatactacg aatgggttga
     1801 gtttgcccaa tcggatgatt ataaacgctt tgaagagctt aaaagttggg aagcaaccaa
     1861 ggagtacctc accgtaaaaa aggaggttga ggccattaaa tactccgaat cgcctgaaca
     1921 aagggaggag caggagctaa aaaaactttc ccgcagccac tccatcagaa acttcctcaa
     1981 aatagccaaa tccgaaaccc ccgattttta ccagaaaatg gagcattccc agctggtaaa
     2041 ggaactaaag gaattgcagc aattcattgc aactcctgat tatcaagcca aaaaaaatca
     2101 gtatagaaaa gataactcag cagagtacca gaaggaactt agactcaagg agttgctaag
     2161 taatcccgat attaaaaagt atttaaaact tgaaaaggag aatgatttaa aagagtactt
     2221 tcaaactgaa aattccgaac aactcaagcg gtacaacgag ctatccgaaa aggttagcac
     2281 cgaagatttt aaaaaccgta aggcctacct gcttagtaag aataagtttg agcaaaccga
     2341 tgcctacaag aaccttcagg aatttaagca gctggagtcg tcggaaaaaa taaaatggtt
     2401 cttccggctg aaccgcagca ataaatttga tgacctgaaa gcatggaacc tagcttttta
     2461 cgatgatttt gactcaaaaa atattgaccc ccacaagtgg ctcacgaagt tcttttgggg
     2521 cgaagccctt gttggcaaac cgtactcttt tactaccgac aaccagaact acaccgaaaa
     2581 gggaaatatt gaaattggca acagcaccct aaagattatt acccgcaagg aggccagcga
     2641 ggggctggca tgggataaaa agtttggatt tactccaaaa gcatttgcct atacctcggg
     2701 tataatcaac accggccaca ctttcaggct taaggaagga agaattgagg caaaggttcg
     2761 tatgagcagt gtgcagggta ttagccacgc cttttacctg gtgggcaacg agatgcttcc
     2821 agaggtggat gtattcctga aaaataacgc taagccgagt ggcatttcag cagcttactt
     2881 ctggcctaac ggcaagagca aagtagccaa atcggtatcc tccattggtg ggttaaagct
     2941 aggcgagaac ttctacatct ttggtgttga atgggattcg aacaacattg tatggaccat
     3001 caacggcatc ccgtataagg ttgagcccaa ccgccacccc ggtatctcct actacatggt
     3061 atttacttct gcagttaaag gaagcgttga cgactccctg cttcccgcca cccttgaact
     3121 cgactgggta aagtgctggg agaaaaagga ataacgttaa actctctacg caaatagcta
     3181 ccactgccta gccgttaccc gaagcacggt tttaaagtcc tcatggtagg tcatctgggt
     3241 atcaaactgc cagctccact ccctgatggt gcaaccgggt attctgacaa aaacagttgg
     3301 attagcgcca accaggtttc catcttcatc gtagtactcc tcctcgtagt cggaaaccag
     3361 caacgtgcgc cccactggaa gctggtcaac atgcctttgc tgaatctgtt ccgaaagctc
     3421 gtggagctgc tcgttactca gcttcccctt atgcctgaga taggctatag gaatatcgtt
     3481 tagctggctg agaaggttga gcgaaacaac caaatcctca tcaaacgtgg gcagggtgat
     3541 gttcctaata aagttctcga gttcaaaaaa tgtcagcttt cgggcatggc gttgggctat
     3601 aaaatcgacc aggccaccgg taaggtcaac cgtttcgaaa gatacggagc tagcaccgta
     3661 aaacctatta actacctggg gtgggtggat aatatcggta agaaggagcg tgctgccatg
     3721 ttgtagaatc tcatcaacag gaacatcgag caggttgccg cttccgagta tcctcacagt
     3781 tttggctttt tgccgcagca aactttcaat gattatctgc ctggaatttt ccagaagagc
     3841 tatccag
//
LOCUS       DAOOQX010000113         3842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_28300_length_3842_cov_3.945603, whole genome shotgun sequence.
ACCESSION   DAOOQX010000113 DAOOQX010000000
VERSION     DAOOQX010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28300_length_3842_cov_3.945603"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1375
                     /locus_tag="PLX53_02725"
     CDS             203..1375
                     /locus_tag="PLX53_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOY72022.1"
                     /translation="MKRISITLMLLAPVIAGNAQQVTEAVSMFAFDLYAKTAAGNSSN
                     FVFSPLSITPAFGMVALGARGETQKQLDEVFHFQPTTNFHSELGKLQQKVLKSAGDSV
                     EICMANRAWLEKSYRVLGKYSKALRRSYSAKIATADFVGNFEASRLLINKTVEADTRS
                     YIKNLLPEGSLDEMTRLVLTNAIYFKGKWEVPFDPKRTSARNFNLSSGETVKCPTMLA
                     DDKFGYYVGTNYQALELNYRGNKLSLLILLPNEDTPMADFERNLSLQLYQQATKGLNP
                     EKVTVLLPKFTIETSLSLKQTLSDMGMPIAFSNAANFSGISGKDDLKISDAFHKAYIE
                     VSEEGTTAAAATAVVVAVKSMLPANTFVANRPFIYILRHKDSGAILFMGKVENPLR"
     gene            complement(1496..2197)
                     /locus_tag="PLX53_02730"
     CDS             complement(1496..2197)
                     /locus_tag="PLX53_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOY72023.1"
                     /translation="MKIGTLVNDFINLFYPNICAVCHDGLARGEEVICTQCLYHIPRT
                     RYWLETDNPVARLFWGRVNIENACSLFLFAKGSKYRKLIHQLKYNGREDIGVFLGREF
                     GKELCRAELYKSVNFIIPVPLHPRRQKERGYNQAEVIANGLSKSMGIPVIPDVLVRRV
                     YTQTQTKKSRTERVQNVSGAFDLQNPEKISKHHVLLVDDVITTGSTLEVYAQTLLRVP
                     EIKVSVGTLAYASSW"
     gene            complement(2300..3055)
                     /locus_tag="PLX53_02735"
     CDS             complement(2300..3055)
                     /locus_tag="PLX53_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOY72024.1"
                     /translation="MSMRGMLAFLFTSLLIVRVGYAQVGFTQEGVASFYGDEFEGRVT
                     ASGERYSHNKATCAHLSIPFGSLVKITNLDNGQTAIVRVNDRGPFVPDRVVDVSRSVA
                     ERLGMIPTGVARVKVEVVDANAPLPANQNAIEEVPAKPKVGTVKVAGSSSTSKTSTAQ
                     GEGEFYQLKVTPVKPDQYGIQIGSYKEPVNVFKIASDFQKLLKQSMMINVVNVNGEKV
                     YRLLVGGFETRKEAEEVKNKIQNDYPGCFVVGF"
     gene            complement(3066..3734)
                     /locus_tag="PLX53_02740"
     CDS             complement(3066..3734)
                     /locus_tag="PLX53_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOY72025.1"
                     /translation="MGTCIEASTLNFLADLQGNNNREWFQQNRNRYELAFSNFYQLVL
                     SLIAGIGSFDKNLGSPDPKDCIFRIYRDVRFSPNKMPYKQNFGAYIAKGGKKSNLAGY
                     YIHIEPHESFAAGGIYMAPSDIMRRVREDIYDYPEDFLSIVNSKEFRGKFSFEDAESL
                     GKVPRGFEAGTAVDEYLKLKNLIPSISFSDSSIQKETFVDEVLQVYKALQPLVGFINQ
                     AINQ"
BASE COUNT         1039 a          967 c          787 g         1049 t
ORIGIN      
        1 aaagcattta acgataacga gctggttaca gcctgcatgt ggactattga ggattacttt
       61 ttcgagtaaa agcatacagc aaattcatta actttggcac ttttacggcg aacatgcggg
      121 aagcacagag aaaaaacaat tcctgcaagg caggcttatg acttgcataa cttttgttgt
      181 aacttggtaa aaaattacta ccatgaagcg gatatccatt actttgatgt tgctggcccc
      241 ggtaatagcc ggtaatgccc agcaggttac tgaggctgtc tcaatgtttg ccttcgacct
      301 ttatgcaaaa acagctgccg ggaacagcag caactttgtt ttttctcccc taagcattac
      361 tcctgctttt ggaatggttg cgctaggcgc aaggggcgaa acccaaaaac agctggatga
      421 ggttttccac ttccagccaa caaccaactt tcattccgag ttgggtaaac tccagcaaaa
      481 ggttttaaaa tcggccggcg actcggtgga aatttgcatg gccaaccgcg cctggctcga
      541 aaaatcatac agggtgcttg gcaagtacag caaggcgcta cgacgctcat acagcgctaa
      601 aattgccact gccgattttg ttggaaattt tgaagcctca cggttgctga tcaacaaaac
      661 tgttgaagcc gacacaagga gctacattaa aaatcttctc ccggaaggta gcctcgacga
      721 gatgacccgc ctggtgctaa ccaatgctat ctacttcaag ggtaaatggg aagtaccgtt
      781 cgatcccaaa cgaacttcgg caagaaactt caacctttca tcgggtgaaa cggtgaaatg
      841 tcctaccatg cttgccgatg acaagtttgg atactacgtg ggaaccaact accaggcact
      901 ggagctaaac taccgcggga acaaactttc gctgctaatc cttctgccca acgaggatac
      961 acccatggcc gattttgaga gaaatctttc actacaactc taccaacagg ctacaaaggg
     1021 gctgaaccct gaaaaagtta ccgtgctcct gcctaagttc accattgaaa cctccctcag
     1081 cctaaagcaa acgcttagcg acatgggtat gcccatagcc tttagcaatg ctgccaactt
     1141 ttcgggaatt tcaggcaagg atgacctgaa gatttccgat gcgtttcaca aggcttacat
     1201 cgaggtttca gaggagggta caactgctgc tgctgccact gctgttgtag tagccgttaa
     1261 atcgatgcta ccggccaaca cctttgttgc taacaggcct tttatttaca tccttcgcca
     1321 taaggattcc ggtgccattc tctttatggg caaggtggaa aatccactta ggtagaattt
     1381 caacctccaa gcggccagtt gagccgcaaa agttactgtt gccggttgaa ttggctgtat
     1441 gaagtatgca gggcgatttt tacccctgca tttttcaaat taccctatat ttagtttacc
     1501 atgacgaagc gtaggctagt gttccaacgc tcaccttgat ttcaggaact cgtagaagtg
     1561 tctgagcgta cacctccagc gttgaccctg ttgtaattac atcgtcaacc agcaaaacgt
     1621 gatgcttcga aatcttttca ggattctgaa ggtcaaaagc tcccgaaacg ttctgaactc
     1681 tttcggttct cgactttttg gtctgggttt gggtgtaaac ccttcgcacc agcacatccg
     1741 gaataaccgg tattcccatt gactttgaaa gcccgttggc aatcacctct gcctggttat
     1801 agcccctttc cttctgcctc ctgggatgaa gcggtacggg tattatgaaa tttaccgatt
     1861 tataaagctc tgccctgcag agctcctttc caaactccct gcctagaaaa acaccgatgt
     1921 cttccctccc gttgtatttt agctgatgaa taagctttcg gtacttactc ccctttgcga
     1981 ataggaatag cgaacaggca ttttcaatat ttaccctccc ccagaatagc cgcgccacag
     2041 gattatcggt ttccagccag tatcttgttc tgggaatatg gtataaacat tgagtgcaga
     2101 taacttcctc accccttgcc aacccgtcat ggcacacggc gcagatattc gggtaaaaca
     2161 ggtttataaa atcgtttacc aacgtaccta ttttcattcc cttaagcttt gcaataaccc
     2221 aacctactcc tgaggtagga aattaactct taaaatgccg aataccctaa ttacttacta
     2281 cccactgcaa tttttaatgc taaaacccaa ccacaaagca gcccgggtaa tcgttttgaa
     2341 ttttattctt tacctcctcg gcctccttcc gggtttcgaa tccacctact agcaggcggt
     2401 aaaccttttc gccattaaca ttaaccacat taatcatcat gctctgcttc agcaatttct
     2461 ggaaatcgct tgcaatctta aaaacgttaa ccggctcctt gtagctgcca atttgaatgc
     2521 catattggtc aggcttcact ggtgtaacct taagttggta aaactccccc tcgccttgag
     2581 ccgtggaagt tttggacgtg ctggatgagc ctgccacctt aacagtacca acctttggtt
     2641 ttgcgggtac ttcctcaatc gcattctggt tggctggcag gggcgcattt gcatcaacca
     2701 cttcaacttt aacccgggct acaccggttg gaatcatacc cagcctttcg gcaaccgaac
     2761 ggctaacgtc aaccaccctg tcgggcacaa aaggccccct gtcgttaacc ctaacaattg
     2821 ctgtttgtcc attatccaaa ttggtaattt tcaccagcga gccaaacgga atggacaggt
     2881 gcgcacaggt agccttattg tggctgtacc tttcgcccga agcggttacc ctgccttcaa
     2941 actcatcacc gtaaaaagat gcaactccct cctgggtgaa gccaacctgg gcatatccaa
     3001 cccttactat taaaagtgag gtaaaaagaa aagcaagcat tcccctcata ctcatatttt
     3061 atactttact ggtttatggc ttgatttatg aaccccacaa gtggctgcag cgctttatat
     3121 acctgcagca cctcgtccac aaaagtctcc ttctgtatgc tgctatcgct gaatgaaatg
     3181 gagggtataa ggtttttaag ctttaagtac tcgtcaactg ctgttccggc ttcaaaacct
     3241 ctgggaactt ttccaaggct ttccgcatcc tcaaaggaaa atttccctct aaactccttt
     3301 gaatttacaa ttgacaaaaa atcttccggg taatcataaa tatcctccct aactctccgc
     3361 atgatgtcgg aaggcgccat gtatattcca ccggcggcaa atgattcgtg tggctcaata
     3421 tgaatatagt agccggccag gttactcttt ttccctcctt tggcaatata tgccccaaaa
     3481 ttttgcttgt agggcatctt gttaggtgaa aaacgtacgt cgcggtatat tctgaaaatg
     3541 caatccttag gatcgggcga tcccaagttt ttatcaaaag agccaatacc ggcaatcaac
     3601 gacagcacca gctggtaaaa attactgaag gccaattcgt aacggtttcg attttgctga
     3661 aaccactcac gattgttatt tccttgtaaa tcagccaaga agtttaaggt tgaagcttcg
     3721 atgcaggtac ccatacagca taatttttac caaaattaat aaaatttgca aactgtagcg
     3781 aatggagctc cctgcaattg gttggtgaaa atggtttaaa acaaaaagcc accttaaggt
     3841 gg
//
LOCUS       DAOOQX010000114         3825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_28478_length_3825_cov_3.476127, whole genome shotgun sequence.
ACCESSION   DAOOQX010000114 DAOOQX010000000
VERSION     DAOOQX010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28478_length_3825_cov_3.476127"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PLX53_02745"
     CDS             complement(<1..794)
                     /locus_tag="PLX53_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72026.1"
                     /translation="MMKSKPNARKLNLQFFSSLSLKSKILILFTGSTILILIAALIFI
                     SNQLKRQSVENAIAQIGITADQQALMIQNALEKNLYIARTLSGTAKSCSQLPPDRLNS
                     YLTSAYRDVLQSEPNILSVWDSWELKFLDPAFSNSYGRIRNMAWRSNGTILTKSETIL
                     TPDSPQYARIKKELTESVEEPYFFSFDNGSPVLMTSLVVPNIIDGKFAGVSGVDIPLT
                     GFNELIRGIDRYEGSYAFVLSTTLKFIAHPDKNLAGDDAMASYEEL"
     gene            complement(815..1651)
                     /locus_tag="PLX53_02750"
     CDS             complement(815..1651)
                     /locus_tag="PLX53_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOY72027.1"
                     /translation="MDSIRKVVLVPWDFTQSSEYALQHAIELAKAGDNEIMLLHIIPH
                     SPLFDIFYKPNFKKIEAINKQFEKVAQDVQSRFGIIPQTLVMEGSPRKIIRGLILTAN
                     VNLIVAEHGYTIKDKTYRTSDFIKNLTFKDITLPFIIVSKPPRHDHYIEIVIPVDYDR
                     KFKEAVHWIIYLAKYYKCNINLIKPFLTDDGQKKQLANNIYFTKKMLDGVGIIYGIKT
                     AKKKSFFKDEVFRFANTIEADLILVMADKYNYFVDAKREANLDIPVMCVNPRIRKYQS
                     FY"
     gene            complement(1784..3127)
                     /locus_tag="PLX53_02755"
     CDS             complement(1784..3127)
                     /locus_tag="PLX53_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOY72028.1"
                     /translation="METLLFQRTNELLKEREKSENLLARVLPQETADELKQFGKIKAQ
                     KFQMVTVLFSDIQGFTKITDDTDSEELIDQLDRFVYEFDSVVQKYHIEKIKTVGDAYM
                     CAGGLPKKNRTNPIEVVVVALEMMSWMKETNRRHQKGDVWELRMGIDTGPVIAGVVGQ
                     SKISYDIWGATVNTASRMESAGEAGKINISENTYLLVRDFFICTYRGQIPVKNRGNIQ
                     MYFVEGILPNLSQNLEGTLPNQDFFIELQLIRLSDLEELVLEKLEQGLPKNLYYHNVK
                     HTVDVYTQVELIGRAEGVSKEDLLLLRTAALFHDAGHLVDYATHEQMGVKLAREILPN
                     YRYSSDQIDEICKLIMSTKLPPNPKNLLEEIICDADLDYLGRTDFIPVSNMLYKEMHE
                     NGMIGSLHEWNILQMNFIQNHQYFTQTARRLRNVNKDMQLQNIKSWINQMEKSNL"
     gene            3574..>3825
                     /locus_tag="PLX53_02760"
     CDS             3574..>3825
                     /locus_tag="PLX53_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOY72029.1"
                     /translation="MKGESKTILVPTDFSQVAEYALQHAVRVSGVVKEPITILHIVKA
                     EADKAEAEARLEKIVRGTKEKYGVDITPVVRVGNIFTDIA"
BASE COUNT         1013 a          807 c          867 g         1138 t
ORIGIN      
        1 aacaactcct catagctggc catggcgtca tcacctgcca agttcttatc gggatgggca
       61 ataaacttca gggtggttga gagcacaaag gcatagctcc cttcatagcg gtcaatgcct
      121 ctgatgagct cgttaaaacc agtgaggggg atgtcgacac ccgacactcc ggcaaacttc
      181 ccatcaatga tgttgggcac aaccaggctg gtcatcagca caggggagcc gttatcgaaa
      241 gaaaaaaaat agggctcctc aaccgattcg gttagctctt ttttgattcg ggcgtactgg
      301 ggtgaatcag gagtaagaat ggtctccgat ttggtgagta ttgttccgtt gctcctccat
      361 gccatgttcc ttattctacc gtagctgttg ctgaaagccg ggtcaagaaa tttgagctcc
      421 cagctatccc aaacggatag gatattcggt tccgattgca gcacgtcccg gtaagcagaa
      481 gtaaggtagc tgttgagcct atcgggtggc agttgactgc aggatttggc agttcccgat
      541 agggttctgg caatatacag attcttttct agggcatttt gaatcatcaa ggcttgctga
      601 tcggcagtaa tgcctatctg ggcaatggcg ttttcaacag attgtctttt tagctgattg
      661 ctgatgaaaa taagagcagc tataaggata agaatagtgg acccggtgaa caggataaga
      721 attttacttt taagtgaaag cgatgaaaaa aactgaaggt tgagtttcct tgcgtttggt
      781 ttactcttca tcataacccg atgtgtggta gagattagta aaaactttga tacttacgaa
      841 tcctggggtt aacgcacata acggggatgt ccaggttggc ctcccttttg gcatcaacaa
      901 aatagttgta cttatcggcc ataaccagta tcaaatcggc ctcaatggta ttggcaaagc
      961 ggaatacctc gtccttaaaa aaacttttct ttttagcggt tttaatgccg taaataattc
     1021 ccacaccatc gagcatcttt ttggtaaagt agatgttgtt ggccagctgc ttcttttgtc
     1081 cgtcatcggt aagaaacggt ttaataaggt tgatgttgca cttgtagtat ttggccaggt
     1141 agatgatcca gtgtacagcc tccttgaact tgcggtcgta atcaaccggg attacaattt
     1201 cgatatagtg gtcgtgcctt gggggcttgc ttacaataat aaagggcagg gtaatatctt
     1261 taaaggttaa attcttaatg aaatcggagg tgcggtaggt tttatcctta atggtgtagc
     1321 catgttcggc aacaatcagg ttcacgtttg ccgtgagtat taaccctctg attatcttac
     1381 ggggacttcc ctccattacc aaggtttgcg ggataattcc aaacctgctc tgcacatctt
     1441 gtgcaacctt ctcgaattgc ttgttgattg cctcaatttt tttgaagttc ggtttataga
     1501 agatgtcaaa gagtggggaa tggggaatga tatgcagcag cataatttcg ttatctccag
     1561 ccttcgccag ctcaatggca tgttgaagag cgtattcgga actttgggtg aaatcccaag
     1621 gaacaagaac tacctttctt atgctatcca taccgagatg ttttgtttat aaaatggcat
     1681 actcattcat gctcaacaat tgcgagtaaa agccaaagtg attaattccc attacatttg
     1741 aataaattca gtaaaagtaa aatattttgt gtaaaaatca tagctaaagg ttggattttt
     1801 ccatttggtt tatccaactc ttgatgttct gaagctgcat gtccttgttt acattcctta
     1861 gccttcgtgc ggtttgggtg aagtactggt ggttttgaat aaaattcatt tgcagaatat
     1921 tccattcgtg aagggaacca atcattccat tttcgtgcat ctccttgtaa agcatgttgg
     1981 aaacggggat aaagtccgtt cgtccgaggt aatccaggtc ggcatcacat ataatttcct
     2041 caagaaggtt tttcggattg gggggtagct tggttgacat gattagcttg cagatttcat
     2101 ctatctggtc ggaagagtac cggtagttgg gtagaatttc cctggctagc tttaccccca
     2161 tttgctcgtg ggtggcgtag tcaaccaggt ggcctgcgtc atgaaacagg gcagcggttc
     2221 ggagtaggag cagatcctcc ttgctaaccc cttctgcacg cccaatcagc tcaacctggg
     2281 tgtacacatc caccgtatgc ttgacgttgt ggtagtatag attttttgga aggccctgct
     2341 caagtttctc cagcaccagc tcttcaaggt cgcttagccg gattagctgg agctcgatga
     2401 aaaagtcctg gttaggaagt gtgccttcga ggttttggga taggttgggc agaataccct
     2461 ccacgaagta catctgtatg ttccccctat tctttacggg gatttgccca cggtaggtgc
     2521 aaatgaaaaa atcccgcacc agcaggtagg tattttccga aatgtttatc ttaccagctt
     2581 cgccggccga ttccatcctg ctggcagtgt taacggttgc tccccaaata tcatacgaaa
     2641 ttttactttg ccctactact ccagctataa ccggcccggt gtctatgccc atacgtagct
     2701 cccacacatc acctttctgg tgccttcggt tagtttcctt catccagctc atcatttcaa
     2761 gcgcaacaac caccacttca atggggttgg ttcggttttt cttcggaagc cctccggcac
     2821 acatgtaggc gtccccaaca gttttaatct tttcaatatg gtacttttgt acaaccgaat
     2881 caaactcgta aacaaacctg tcgagctgat cgataagctc ttcggaatcc gtgtcgtcgg
     2941 ttatcttggt aaaaccttga atatccgaga atagcacggt taccatctga aacttttgcg
     3001 ctttaatctt tccaaattgc ttaagctcat cggctgtttc ctgaggtagt acacgggcca
     3061 gaagattttc cgatttttcc ctctccttca aaagctcatt agtacgttga aatagtaggg
     3121 tctccaagcc atgcatcgcc tttgttcgcg agtagcttat caagctatag agcaaaaaaa
     3181 gggttaacaa cactagtatt gaaccaagcc aaatcattat tcagtaatat gcaggaagat
     3241 ttgccatcta agctacaata tttttaaatg atttgaaatt aatcaggtaa tatttattcg
     3301 tctcgttggc atattcaggt atgtaaaagg ttaaaggata taagccatta aaggaaatct
     3361 ggtttggact gattttttct cttctctcta acttataaca cattgcccct ccagattcat
     3421 ggttcagtaa tttatgccgc aaccccgggg tttaaaataa ccacgaatcc gttaaaagaa
     3481 acccaagttt taaaggtttt attatttttg tatgaaaatt ttataaaact ttggaagtcc
     3541 ggctaaagcc ttgaacattt taaaaatgta agtatgaagg gagaaagtaa aaccattctg
     3601 gtccctaccg acttttcaca ggtggcagag tatgccctac agcatgccgt tagggtttcc
     3661 ggcgtagtga aagaacctat cactattttg catattgtta aagcagaggc cgataaagca
     3721 gaggcagagg ccaggctgga gaaaatagtt cggggcacaa aggagaagta cggagttgac
     3781 atcaccccgg tggttcgggt aggcaatatt ttcaccgata tagcc
//
LOCUS       DAOOQX010000115         3819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_28530_length_3819_cov_3.183316, whole genome shotgun sequence.
ACCESSION   DAOOQX010000115 DAOOQX010000000
VERSION     DAOOQX010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28530_length_3819_cov_3.183316"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="PLX53_02765"
     CDS             <1..1070
                     /locus_tag="PLX53_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOY72030.1"
                     /translation="STEFYNGVIVPGFVNAHCHLELSHLRGKIPKNLGIAGFIKAITE
                     QRGTDEEVQNHGELVERAIAGAIHEMEQTGTVAVGDISNSADTLLAKLQSDIYFHNFI
                     EVFGLNPNEAQSIFEGYLEVYEVFKQKFCGSTSLTPHSPYSISQPLWEKISEFIGKSK
                     ELVSIHYAESAAEYQFLQDGTGPLSDRYQSAGIPFCKPQGLTPRGVVSRYVNRDCKVM
                     LIHNTFAPAEELSWLAAIYPQTTVVTCPESNLLIEGKLPNLPEFETSGLNIAIGTDSL
                     ASSSSLSMLSQINIILQHFPQLTFSNVLQWATLNGAEALGISSTYGSLEPGKNPGLVL
                     ITGFDFRNMCATPQSLAKRLI"
     gene            complement(1144..3090)
                     /locus_tag="PLX53_02770"
     CDS             complement(1144..3090)
                     /locus_tag="PLX53_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOY72031.1"
                     /translation="MRNDKAGKISKLSFVGLIITMGIVYGDIGTSPLYVMRAIASSVT
                     TIDNDFILGALSCIIWTLTLQTTVKYVLITLRADNKGEGGIYSLFALVRRKMPKAYII
                     AIIGGSTLLADGIITPAITVTSAIEGLQIVQKQLPTIPIVLIIITGLFVIQQFGTKKI
                     GSSFGPIMTLWFLTLAILGVNQIVQLPVVFKAFNPYYALQLIVEHPSAMLVLGAVFLC
                     ITGVEALYSDLGHCGLKNIQISWIFVKSALILNYLGQGAYLLKNGFNGNNPFFSIMPQ
                     WFVLPGIILATAAAIIASQALISGSFTLISVASSLNLWPNIKRKYPTNIKGQIYIPSV
                     NWFLFLGVIFVVLFFQESSNMEAAYGLSVNLTLISTTILVAFYLMSKKVNPVLITLFS
                     LSFLLIEGVFLYANLLKFKHGGWLTLTLASLFFIVKYGWYNARKIRNRHITFTSINEY
                     LPIICDLSKDKEVPKYATNLVYMTRANNPDEIESKIIYSILNKQPKRADTYWFLHVDK
                     LDEPDTFAYEVTEMVPGIIRRIDFRLGFKIEPRVNLYFHQVLEEMTALKEIDLVSHYK
                     SLRKYNITADFLFIMIDRVPNYDIDFSPFEKFMMVLYSIFRRLGISEVKSLGLDSTNI
                     IVEKVPLSTHVSYKRKAIKRKIFS"
BASE COUNT         1079 a          819 c          827 g         1094 t
ORIGIN      
        1 attccacgga gttttacaac ggggtaattg ttccgggttt tgtaaatgcc cactgccatt
       61 tggaactctc gcatcttcgt ggtaagatac ccaagaactt gggcattgcg ggttttatta
      121 aggccattac cgagcaaaga ggaacggatg aagaggttca aaaccatggt gagttggttg
      181 aacgggcgat agcgggagct attcacgaaa tggaacaaac cggaactgtt gccgttggcg
      241 atatatcaaa ctccgccgac accctcctgg ctaaacttca atcggatata tacttccaca
      301 actttattga agtatttggg ttaaacccca acgaagccca atctatattt gaaggttact
      361 tggaagttta cgaagtgttt aagcaaaaat tttgtggttc tacctcgtta accccccatt
      421 cgccctactc aatttcacaa cctttatggg agaagatttc agaatttatc gggaaaagta
      481 aagagctggt tagcattcac tatgccgaaa gcgctgccga ataccagttc cttcaagacg
      541 ggactggtcc cctcagcgac aggtaccagt cggcaggaat tcctttttgt aagccccaag
      601 ggctaactcc ccgtggagtt gtgtcccgct atgttaatcg ggactgcaag gtgatgctta
      661 ttcacaatac ctttgctcca gctgaggagc ttagctggct tgctgctata tacccacaaa
      721 caacagtagt tacgtgtcct gaatcgaacc tgctaattga agggaaacta cccaacctcc
      781 ccgaattcga aaccagcggc ctcaacattg ccattggcac ggacagcttg gcttcatcat
      841 cttcgctttc catgcttagc cagatcaata tcatccttca gcacttccca cagcttactt
      901 tctcaaatgt tttgcaatgg gctaccctta acggagccga agcactgggt atttcgagca
      961 catacggaag ccttgagccg ggtaaaaatc ccgggttggt tctcataaca ggttttgatt
     1021 ttcgaaatat gtgtgctacc ccccaaagcc ttgccaaaag gttgatttag cccatgtggt
     1081 tggtgaaagg ataagtttat acactgcttg ctacccaaaa gtatgggggc aggattcaac
     1141 cccttatgag aagattttac gcttaatagc ttttcgtttg tagcttacgt gggttgatag
     1201 cggaaccttc tctacgatta tgttcgtgct gtctagtcca agcgacttaa cctcgctgat
     1261 gcccagcctg cggaatatcg agtaaagcac catcataaac ttttcgaagg gtgaaaaatc
     1321 gatgtcgtag tttggaacac ggtcaatcat gatgaagaga aagtctgctg ttatgttata
     1381 cttccttagc gatttataat ggctaaccag gtctatctcc tttagcgcag tcatttcttc
     1441 gaggacctgg tgaaagtaaa ggttaactct tggctcaatt ttaaaaccca gcctgaaatc
     1501 tatccttctg ataattccag gcaccatttc cgtaacctcg tatgcaaaag tatcaggttc
     1561 gtcgagcttg tcaacatgta aaaaccagta ggtgtcggca cgcttaggtt gcttgttcag
     1621 aatggagtaa ataatttttg attcgatttc atctgggttg ttagcacggg tcatatacac
     1681 caaatttgtt gcgtatttag gaacctcttt atccttactc aggtcgcaaa ttataggtag
     1741 gtactcgttg atactagtga aggtgatatg gcgatttctg attttacggg cgttatacca
     1801 gccgtatttt acgatgaaga aaaggctggc aagggttagc gtcaaccagc ctccgtgctt
     1861 aaacttcagg aggtttgcat acaggaatac cccttcaatc aaaaggaatg acaggctgaa
     1921 taaggtaatc aggactgggt ttaccttctt cgacatcagg tagaaagcaa ccagaatggt
     1981 tgtggaaatc agcgtaaggt taaccgatag tccgtaggca gcttccatat tggacgattc
     2041 ctgaaaaaag agcaccacga agattacccc gagaaacaga aaccagttta ccgagggaat
     2101 gtatatctgt cccttaatat tggtagggta ttttctcttg atgttgggcc ataggtttag
     2161 ggagcttgca acgctgatca gggtgaatga accggatatt aaggcctgac ttgcaataat
     2221 ggcagctgcg gttgcaagaa taatacctgg aagcacgaac cattggggca taatcgaaaa
     2281 aaatggatta ttgccgttaa aaccattttt taacaggtag gcaccttgcc ccaggtagtt
     2341 aagaattaaa gccgacttta cgaatatcca gctaatttga atgtttttca acccacaatg
     2401 ccccaaatcg gagtagagcg cttctacccc tgttatacaa agaaatactg cacccaatac
     2461 cagcatggcc gatggatgct ctacaattaa ttgcaaggcg tagtagggat taaacgcttt
     2521 aaacaccacg ggtagctgaa caatctgatt tactcccagt atcgccagcg ttaaaaacca
     2581 tagtgtcatt atggggccaa atgagctacc gattttttta gtgccaaact gctggataac
     2641 aaaaagtcct gtaattatga tgagaacaat gggaatagta ggaagttgct tttgaacgat
     2701 ttgtaaacct tcgatggccg atgttacggt aattgcaggt gtaatgatac cgtcagccag
     2761 gagggtgctc cccccgatga tggcaataat atacgccttt ggcatcttcc tccttaccag
     2821 cgcaaacagg gagtagatgc ctccctcacc tttgttgtcg gctctaaggg tgatcagaac
     2881 atacttaacg gtggtttgaa gggttagcgt ccagataatg caggacaacg cacccagtat
     2941 gaagtcgttg tctatggtag ttacgctgct ggcgattgcc cgcatcacgt aaagcgggga
     3001 ggtgccaata tctccataca caatacccat ggttatgatt agcccgacaa agcttaattt
     3061 cgatattttc cctgccttgt catttctcat aagtcaacct ttaattttta tacctaacat
     3121 ttatactatt gctatcataa aaatgtaatc cggcggtcag cttcacgttg aaccttagaa
     3181 tttcatctac tcgctaaatc ttgcaaccca acgctttact agtgcacaaa tatcaagatt
     3241 attagggtta atcatgattt tatttggagt agcgatgacc ttttgggtat ctatctatac
     3301 tgatgggtga attgccaggt taaaccagta cggttgttta aagattacaa gactttaact
     3361 ggtaaaacag cctcattaac aacagcatga acttatttta agctaaaatg ggtaattttt
     3421 tgaacccgca aaagtataaa agaaggccat aggatgtcat caatttataa actttttatt
     3481 agaacaaatt tttaaaggta taagcctgct ataatgctat ttatgagatt acgcccgatg
     3541 aactaagacg gctattattc ctgcactgtt ccggaagcca tgactttaag ttgttgccat
     3601 gttagctcat accattgggg cgacccataa cggattaatt ctacatttgc cagcctatct
     3661 gacctgtatt ccggcgattt acattcaaat ttccttgtaa attagctttc agccaaatat
     3721 ctttgcagga gcaacactct tctgattcag ccaccgattg gagttgtttt atgctacgat
     3781 taaacctatg tttgatgggt aatatagaag ttgtggcgt
//
LOCUS       DAOOQX010000116         3815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_28565_length_3815_cov_3.693617, whole genome shotgun sequence.
ACCESSION   DAOOQX010000116 DAOOQX010000000
VERSION     DAOOQX010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28565_length_3815_cov_3.693617"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLX53_02775"
     CDS             <1..192
                     /locus_tag="PLX53_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72032.1"
                     /translation="LNDDNGIVNLRSRDFKLRLLDRSKASQSRLAWQVVNTVLPSLVL
                     VLGGLIYFFIRRNHYSTPK"
     gene            483..1613
                     /gene="dnaN"
                     /locus_tag="PLX53_02780"
     CDS             483..1613
                     /gene="dnaN"
                     /locus_tag="PLX53_02780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOY72033.1"
                     /translation="MKFVVSSTELLSHLSVVSRVISSKNTLPILDNFLFNLEGNELEI
                     TASDLETTLVTRFSLENVSDEGTVAIPYKILIDTLKEFSEQPLTFDINPGTQNITINS
                     ENGQFHIVGQPADDFPQRAMLKPEDSHSVKLSAEILLNGINKTIFATADDEMRPMMNG
                     IFTELNEQGITFVSSDAHRLVRYRRKDVQVEADASFILPKKPAQLLKNMLVKETGFVN
                     VQFDDKNAVFTMPGYTMVCRLVEGNYPNYNAVIPVDNPNRMIVDRVDLYNSVRRVSIY
                     SNAASNMVKLSLTGNQCTVSAEDLDFSVSAFERLSCEYEGTEMEIGFKSIFLSEILAN
                     ISSTEVVLSFSEPSLASLVFPHINENPDEDILMLLMPMMIGD"
     gene            1789..2556
                     /locus_tag="PLX53_02785"
     CDS             1789..2556
                     /locus_tag="PLX53_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOY72034.1"
                     /translation="MKLNIKNPLAVIDLETTGIDIVHDRVVEIAVLKILPDGSENMRV
                     RRVNPGMPIPPEATAIHGITDEDVRNEPTFKEIARSLANHLEGCDFAGFNSNKFDLPL
                     LAEEFLRAGVDIDLKRRKFIDVQTIFHKMEKRTLGAAYKFYMDKELTDAHTAGADTMA
                     TYEILMAQLDRYPELKNDVNYLSEFSSFNRNVDFAGRIILDDRGVEVFNFGKHKGLPV
                     VEVLKKEPSYYSWMMNGDFPLYTKRVLTAIYLKMKQQ"
     gene            complement(2579..3526)
                     /locus_tag="PLX53_02790"
     CDS             complement(2579..3526)
                     /locus_tag="PLX53_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOY72035.1"
                     /translation="MERLTEVEKEKILFIINPISGIRPKGNVEKNILKYLDNVKYRAE
                     FHVTTCKGDATAAVQAKLAEGYTKFVAVGGDGTVNEVASAIVGTEATLGIIPTGSGNG
                     LARHLRIPLNLKRAMAIINCGAADRIDYGMVNKIPFFCTCGVGFDAQIGYRFASNGKR
                     GFFNYIKIVISDFLRYKSKKYKLKIDGDGKFKIRAFLITVANSSQYGNNAYIAPLADI
                     RDGKLNLSIISPFKIFEAPVIGIRLFTKRIDKSTMEHSGAISEVVIKRKHKDVVHYDG
                     EPIIMGKKLKISVVPRGLRVIVPATKERIKHPFASRHEP"
BASE COUNT         1027 a          858 c          823 g         1107 t
ORIGIN      
        1 ctgaatgatg ataatgggat agttaacctt cgctcacgcg acttcaagct caggctgctt
       61 gacaggagca aggcttccca atcgcgcttg gcatggcaag ttgttaacac cgttttgcca
      121 tcgctagtgc tggtgctagg tggcctaatc tattttttta tccgaagaaa tcattattct
      181 accccaaagt aatgccaacc ctataacgta gttagggcgc ggctaatatc cgtccctact
      241 tctaaaatgt ggcaaaccat tagaataagc cacatttcaa gaaaaaatat taaataggta
      301 ggctttttaa actttaaacg gacaaaaatt cgtaatattg aacctgaaaa tttaagtaga
      361 tcattctggt aatcagtaat ttttaaattt tggcgcctaa cttctttaag tatggcaagg
      421 gtgtttttac tctcaaaaga taagtaaaag tagcattgat aataacttaa aacttacgaa
      481 gaatgaaatt tgtagtatca agcactgagc tattaagtca cctttcggtg gtaagcaggg
      541 tgattagcag caagaatacg ctaccgattc tggacaactt tctgttcaac cttgagggga
      601 acgagttgga aattactgcg tccgatttgg aaaccacgct ggtaacccgc ttttccctcg
      661 aaaatgtttc cgatgagggc actgtggcta ttccctacaa aattctgata gatacgctaa
      721 aagagttttc cgaacaaccg ttgacttttg atataaatcc cggtacccaa aacatcacca
      781 ttaactctga aaacggtcaa tttcacattg ttggacagcc tgccgatgat tttcctcaaa
      841 gggcaatgct gaaacctgaa gattcgcata gcgttaagct ttctgccgaa attctactca
      901 atggcattaa caaaaccata tttgccactg ccgatgacga aatgcggcct atgatgaacg
      961 gcatatttac cgagctcaac gaacagggaa taaccttcgt atcatccgat gcacatcgct
     1021 tggttcgcta ccgcagaaag gacgttcagg ttgaagcgga tgcctcgttc attctaccca
     1081 agaaacctgc ccagctactc aagaatatgc tggtaaagga aaccggtttt gttaacgtgc
     1141 agttcgatga taaaaatgcg gtgtttacca tgcctggcta caccatggtt tgtcgcctgg
     1201 tcgaaggtaa ctatcccaac tataatgccg ttatccccgt tgacaaccct aatagaatga
     1261 tagttgacag ggttgacctc tacaactcgg ttcgccgggt ctccatctac tccaacgctg
     1321 ccagcaacat ggttaaactt agcctcaccg gaaaccagtg cactgtttcg gccgaagacc
     1381 tcgatttttc tgtttcggca ttcgaaaggc taagctgtga gtacgaggga actgaaatgg
     1441 aaattggatt taagtcaatt ttcctatccg aaatattggc caacatttcc tctaccgagg
     1501 tggttctttc tttctcagaa ccatctcttg cctcacttgt ttttccccac atcaacgaaa
     1561 accccgacga ggatatattg atgctgctta tgccaatgat gattggcgat taagctagag
     1621 cttaccaggc accccggagc ttttaattcc ggggaatttt ttcgggttgc atggtaggct
     1681 tgtcgcatat tcgacccact taagggtttg atttaccttt aatgcaaggt taaccattta
     1741 tatttgatta agattagcgt ttattgcacc atttcaacag ctattgccat gaagcttaat
     1801 ataaagaatc cgctcgccgt tattgatttg gaaacaacag gaattgatat agtgcacgac
     1861 agggtggttg agattgcagt tcttaaaatt ttacccgatg gctcggagaa tatgagggtt
     1921 cgtcgggtga accccggaat gcctattccg cccgaggcta ctgccattca tggcattacc
     1981 gatgaagatg taaggaatga accaacattt aaggaaatag ctcgctcgct ggctaaccat
     2041 ctggagggtt gcgattttgc aggattcaac tctaacaagt tcgatcttcc actgctggcc
     2101 gaggagttcc tgcgtgctgg tgttgatatc gacctcaagc gtcgaaagtt tattgatgtg
     2161 caaaccattt tccacaagat ggaaaagcgc accctgggcg cagcctataa gttttacatg
     2221 gataaggagt taactgatgc ccataccgcc ggagccgaca ccatggctac ctacgaaata
     2281 ctcatggctc agcttgaccg ttaccccgag cttaaaaacg acgtgaacta cctgtccgag
     2341 ttcagctcgt ttaaccgcaa tgttgatttc gccggccgaa tcattttgga tgacagagga
     2401 gttgaagtat tcaactttgg taagcacaaa gggttgcctg ttgttgaggt gttaaaaaag
     2461 gagccaagct actactcgtg gatgatgaac ggcgatttcc ccctttacac taaaagggta
     2521 cttacggcca tttacctgaa gatgaagcag cagtgatagt gtaaagggct aatatccctt
     2581 agggttcatg gcgtgaggcg aatggatgtt taatacgttc tttagttgca ggaactatta
     2641 cccttagccc acgaggtact acgcttattt tcaacttctt gcccattatt attggctcac
     2701 catcgtaatg caccacatcc ttatgcttac gcttaattac cacctctgaa atggcaccgc
     2761 tgtgctccat agtactttta tctatccgct ttgtaaagag ccttatgccg ataaccggag
     2821 cctcaaaaat cttgaaaggg gagatgatgc ttaggtttag cttcccgtct cttatgtctg
     2881 ccaagggtgc aatgtaggca ttatttccgt actgtgatga gttggcaaca gtgataagga
     2941 atgccctaat cttaaacttc ccatcaccat caatctttag cttatacttt ttcgatttgt
     3001 agcgtagaaa atcggagata acaattttta tgtagttgaa gaatccgcgt ttgccattac
     3061 tggcgaacct gtagcctatt tgagcatcaa acccaacccc gcaggtgcag aagaatggaa
     3121 ttttattcac catcccgtag tcaatcctat cagctgctcc gcagttgatg atagccattg
     3181 cccttttcaa gttcaacggg attctcaaat gacgggcaag cccgtttcca gaacccgttg
     3241 gaattatgcc cagtgtagcc tcggtgccaa caattgcgct ggctacctcg ttcactgttc
     3301 cgtcgccccc aacagcaacg aatttcgtat atccttcggc aagtttagct tgaacagccg
     3361 ccgttgcgtc tcctttgcat gtggtaacat gaaactcagc ccggtatttt acattgtcga
     3421 gatacttgag tatattcttt tccacgttgc ccttgggcct tatgccggaa atcggattga
     3481 tgataaataa aattttttcc ttttctacct ccgttaaacg ctccatgggt ttgttgtttg
     3541 ctgggttgtt gcggttggtg caaatgtaag ttcaaaagag gaatagtgca acagatgatg
     3601 attttgcatg gtttcattgg cttcattttt attgctgctc ttgcctcaca caaataattt
     3661 tacttttttt gtcggctaat tagttttcct atgagcacaa ccgatagcag caggcgagta
     3721 gagctgattt ctaacaggat gggtataaag cccaggcagg tttttaacgt tatccacctg
     3781 ctggaggagg gcgcaaccat tccgtttatt agccg
//
LOCUS       DAOOQX010000117         3810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_28607_length_3810_cov_3.355792, whole genome shotgun sequence.
ACCESSION   DAOOQX010000117 DAOOQX010000000
VERSION     DAOOQX010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28607_length_3810_cov_3.355792"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PLX53_02795"
     CDS             complement(<1..438)
                     /locus_tag="PLX53_02795"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOY72036.1"
                     /translation="MKLTKYFAATVVLIAMVTLSLPGCVDFSFDDPQPSIDSTSMSAN
                     TTIAELKSVYPGSLFQLSDTTFYLRDSIIIEGTVISDDKEGNFYKSIFIEDSTGGIEV
                     KLNKTTLYNDYKRGQKVVVLCNDLYLGDYGGQIQLGSTYKNNGV"
     gene            complement(470..3193)
                     /locus_tag="PLX53_02800"
     CDS             complement(470..3193)
                     /locus_tag="PLX53_02800"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY72037.1"
                     /translation="MKRLLTTVLSLFLLGMGSLYAQNTLRGVVKDSDTDTPVQDVRVR
                     LLGTTFETLSNAGGEFTLEGVPDGKFTLEFSIVGYQTITQEVSLPENSQVPVVSLRRE
                     VVADNGSQEFSEVVISADDLDSDNMDQAISGLLQSSQDAFNSAVSYTFSPASFKIRGY
                     DSDYSLLYMNGVPVNDPEIGFASWSSWGGLNDVVRSRESRNGFLPTNFSFGGLGGATN
                     LNVRASQQRVGTRLSYAATNRTYNNRIMFTHSTGVMENGVSFTISGSRRWAEEGYVDG
                     TNYDAWAYFIGIEKKFNSQHSVAFTTYNAPTKRGMQGVSTEEAKNLAGSSYYNPNWGY
                     QNGEKRNSRVRFQQEPTFILNHYWNMLPNLALTTTAGYSFGTYQTTALNWYDAQDPRP
                     DYYRKLPSYWIDSDPDVSQAIADAFSNDPAISQIDWGYLYQVNYNSLDTASNLLRSKY
                     IVENRITDSRQFSLTSVASWNPTQEMKVNGGIEMTLYKGRNYKQIDDLLGGNYWLDID
                     QFVERDYFDNANLAQNDLDNPNRMVAKGDVFGYDYTANVNNGNLWAVANYTLNRFEYY
                     LGGSYTYTEFWRTGDMRNGRFPSNSKGDSPKQQFNDFGVKGGATWKISGRHYLDANMA
                     YLTRAPFFRNSYISPRTSNFVIPGLGSEKIASGDINHNLRTPYIKARITAYYTRFYDQ
                     TELKSFYDDYYRTFINYTMRNIDKEHKGFEIGAEIKASPTLTVSTAAALGSYQYKSRP
                     LATITQDNLGSVLAVDQVVYIKNFFVPNTPQTAAMVGLRYASPKYWFLGATFSYFDDI
                     YIDFAPGKRTAEALVGLEPNDPRRYDLTHQESVSSAYLLDANIGKSWRIKNYYINLNF
                     QVANILDNTDFKTGGFEQARYSIAEQTSDKFPPKYFYAYGRTYYLIFGFRF"
     gene            3493..>3810
                     /locus_tag="PLX53_02805"
     CDS             3493..>3810
                     /locus_tag="PLX53_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOY72038.1"
                     /translation="MYRLFLRNVLAVFLMLVALGYSEAQEKKTYKVSCIGFYNLENLF
                     DTLPGPNDVEFTPDGPNKWNSERYYHKLNNMAEVIAQIGDEMFPGGPAVLGVSEIENR
                     SVLD"
BASE COUNT          988 a          847 c          936 g         1039 t
ORIGIN      
        1 taccccattg tttttgtagg ttgagcctag ctgaatttga ccaccgtagt cacccaggta
       61 caggtcgttg cagagcacca caactttttg accgcgcttg tagtcgttat acagggttgt
      121 tttattgagc ttaacctcaa ttccacccgt actatcctca ataaaaatgc tcttgtagaa
      181 gttaccctcc ttgtcatccg aaatcaccgt tccctcgatg attatagaat cgcgaaggta
      241 gaaggtggtg tcggagagct ggaaaagaga gccaggataa accgatttaa gctcggcaat
      301 ggtggtgtta gccgacatgg aggttgaatc aatgctgggc tggggatcgt cgaaggagaa
      361 gtcaacacaa ccgggcagcg atagcgttac catggcaatc aggactaccg ttgctgcaaa
      421 gtatttagtc aatttcattg ttctcatcat aattattata caagttgagt taaaatctaa
      481 agccaaaaat caggtagtat gtgcgaccgt aggcgtagaa gtatttagga ggaaatttat
      541 cggatgtttg ctcggcaatg gagtagcgcg cctgttcgaa tcctcctgtt ttaaagtcgg
      601 tgttgtcgag gatatttgcc acctgaaagt tcaggttaat gtagtaattc ttaatcctcc
      661 acgattttcc aatgttggca tcaagcaggt aggccgacga aacgctttcc tgatgggtaa
      721 ggtcgtaacg acgggggtcg ttgggctcaa ggccaaccag ggcttcggct gtacgtttac
      781 ccggagcaaa atcaatgtag atatcatcga agtagctaaa ggttgctccc aagaaccagt
      841 attttggcga agcatacctt aagcctacca tggcagccgt ttgtggggtg ttggggacaa
      901 aaaagttttt gatatacacc acctggtcaa cggccagcac cgaacccagg ttgtcctgtg
      961 taatggtggc aaggggtctt gatttgtact ggtatgaacc cagcgctgct gcagtgctta
     1021 cagtaagggt aggcgatgct ttaatttcag ccccaatttc aaaaccctta tgctccttgt
     1081 caatgttccg catggtgtag ttaatgaagg tgcggtagta gtcgtcgtaa aacgatttaa
     1141 gctccgtttg gtcatagaaa cgggtgtagt aggcggttat tctagcttta atgtagggtg
     1201 ttcgcaagtt atggttgata tcgccgctgg caatcttctc gctgcctaat cctggtatta
     1261 caaagtttga tgtgcgcgga gagatgtagg aatttctgaa aaatggggca cgggtaaggt
     1321 aagccatgtt ggcatcaagg taatgacggc ctgaaatttt ccaggttgcg ccacccttta
     1381 ccccaaaatc gttaaactgt tgcttgggag aatcgccctt tgagttagag gggaatcggc
     1441 cattgcgcat atcccccgta cgccagaact cggtgtaggt gtagctaccg ccaaggtagt
     1501 actcgaaacg gttcagggtg tagttggcca cagcccaaag gtttccatta tttacattgg
     1561 ccgtatagtc gtacccaaaa acatcaccct tggcaaccat acggttgggg ttgtccaaat
     1621 cgttttgggc caggttggcg ttatcgaaat agtcgcgctc aacaaactgg tcgatatcga
     1681 gccagtagtt tccacccagc aggtcgtcaa tttgtttgta gtttcggcct ttataaaggg
     1741 tcatttcaat tccgccgttc accttcatct cctgcgttgg attccagctg gccaccgagg
     1801 taagcgagaa ctgacgggaa tcggtaattc tattttctac gatgtacttt gagcggagaa
     1861 ggttggaggc agtgtccagg ctgttgtagt tcacctggta aaggtagccc cagtcaatct
     1921 ggctgatagc aggatcgttg ctaaatgcat cggcaatggc ttgagacacg tcgggatcgg
     1981 aatcgatcca gtagcttgga agcttgcggt agtaatcggg tcgaggatcc tgggcatcgt
     2041 accagtttag cgcagttgtt tggtaggtac caaaggagta gcctgccgta gtggtaagcg
     2101 ccaggttggg caacatgttc cagtaatggt taagaataaa agtaggctcc tgctgaaagc
     2161 gaactcgtga gttgcgcttc tccccgtttt ggtatcccca gtttgggttg tagtagctgg
     2221 aacctgctaa attttttgct tcctcggtcg aaacaccctg catacccctc ttggttggag
     2281 cgttgtaggt agtaaaggct acggagtgct gactgttaaa ctttttttca atcccaataa
     2341 agtacgccca ggcatcgtag ttggttccat ctacatagcc ctcttctgcc caccgacgag
     2401 aaccgctaat ggtgaacgat acgccattct ccattactcc ggtggagtgg gtaaacataa
     2461 tgcggttgtt gtaggttcgg ttggtggcgg cataggaaag ccttgttcca actcgctgct
     2521 gagaggcacg aacattcagg tttgttgctc cgcccagccc gccaaacgaa aagttggtag
     2581 gaagaaaacc attgcgggac tccctgctcc ggacaacatc gttcaaccct ccccatgaac
     2641 tccacgaggc aaaaccaatc tccgggtcgt tcaccggaac tccgttcatg tagagcaagc
     2701 tgtagtccga atcatacccc cggattttga agctggccgg gctaaatgtg tacgaaacag
     2761 ccgagttaaa ggcatcctgc gacgactgta gcaaccccga aatcgcctgg tccatgttgt
     2821 ccgaatccag atcgtcggca gatatcacca cctcgctaaa ttcctgagat ccgttatcgg
     2881 caactacctc gcgtctcagt gaaacaacgg gcacttgcga attctcggga aggcttacct
     2941 cctgcgtaat ggtttgatag cctacaatgg aaaactccaa ggtgaatttg ccatcgggaa
     3001 caccctccaa cgtaaattca ccgccagcat tgcttaacgt ttcaaatgta gtgcctaata
     3061 agcgcaccct cacatcctga acaggagtat ccgtgtcgga atccttcaca actcctctta
     3121 gggtattctg cgcatacaag ctgcccatac ccagcaaaaa caaggacagc acagttgtta
     3181 ataatcgctt catttacaat ttgttattta tttggttgtt gattgatatg tatgaattgt
     3241 ttcacttatc tacattttat ttttagaaat attaacaaca aaaaaatagg tggcggtaca
     3301 ctgacacagc aattatcggg gtgaacaaca aaattaataa gccaacaaat ttcagaaatt
     3361 ttaacctaac caacgggttg gaattatatg atgcaaaaaa agaacatttt tttatacagt
     3421 tgccaaatat tatatctttt tttacggata tagctatctt tgttcggtcg tttatcctaa
     3481 aatcacttga ttatgtatag attatttcta cggaatgtgc tggcagtatt tctcatgctt
     3541 gtggcgttgg gttattccga ggctcaggaa aaaaaaacct acaaggtaag ctgcataggt
     3601 ttttacaacc tggagaatct ttttgatacg ttgcctggtc ctaatgatgt tgaattcacc
     3661 cctgatggcc ctaacaagtg gaattccgaa cgttactacc ataagcttaa caacatggca
     3721 gaggttatag cacaaatagg tgacgagatg ttccccggag ggccagcggt tctcggggtc
     3781 agcgaaattg aaaaccggtc ggtgctggac
//
LOCUS       DAOOQX010000118         3799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_28707_length_3799_cov_3.401442, whole genome shotgun sequence.
ACCESSION   DAOOQX010000118 DAOOQX010000000
VERSION     DAOOQX010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28707_length_3799_cov_3.401442"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..1110
                     /locus_tag="PLX53_02810"
     CDS             400..1110
                     /locus_tag="PLX53_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOY72039.1"
                     /translation="MSYKCVLVDDEPLAVGVLEGFLRHIPDIEVVATFNEAIPLFDFI
                     QHNKVDIIFLDIEMPNLSGIDFLKCLKDPPFVIITSANRDYAVDGYELNVVDYILKPL
                     TMERLVRAVSRAAEALELKARNVTAAPDAEYIFLKEGKKFIRVKVDEIQYIESIKDYV
                     KVVAEGKTVVTRMNISAVEKLLDPAEFIRVHRSFIISKKHIDAYTATSIEIGSVEIPV
                     GRIYRDEAVRRLGYDFEV"
     gene            1302..>3799
                     /locus_tag="PLX53_02815"
     CDS             1302..>3799
                     /locus_tag="PLX53_02815"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HOY72040.1"
                     /translation="MSSRFTLHFNVTMSIYTRYILAVVLALIGYPKMPQAQERGLFLS
                     RYFPASEYNGSTQNWCAVEDNRGVMYFANGQGVLEYDGATWNLIRVDNESAIRSLAAD
                     SSGRIYVGAFGEMGYLYPNRSGQLRYMSLMSLLDKNHSRFSQIWNIYTFGDTAFFLSD
                     SCLYRYCNGKFSYWTSQEGCFYFAFKFNNHLYIQETGVGLQQFDAESLVLVEEGNLLA
                     SQRIHAILPFGNEMLVCTRSNGLFLFRNEGVKNRLTPVSHLSGRALAVDRYFKEYSFY
                     HGVAINDNLLALSSINGNLLMVDTTWLVREVIDYRTKGVKSPVNYLYLQKNNLLWLAL
                     DNGICVVEINSPFRYWTDDMGVNGVVTDVAKLGGFLYVSTQVGNFFLDEKKEENFMPD
                     MFKKVEGDFEQSWEYLYFKVSSNLFPQGHSRPADTLLLVATRTGVYRINGSRSSRISR
                     YEPVFCFHQSKANPAALLVGLSSGIAIVEYRDGFWVDKGFVPGIKENIEQIAEDLDGN
                     VWLKASFSGVYRLKNLFFGSSFKGSLVVEKYDTSSGLPDLKSLYMTSHADSLFFYSQN
                     GYYTFNERLRQFQAYNPANNRRFSAENVDTLAWRRVWNNIISGFYVAHTSDSIIWFNT
                     PKGLFRYTPSSYNRCFDAPATFIRKVVNLDSTLFYGTNFESSKVDSAAGKPFFLPSHN
                     PLVDLGTVLEYKRNSLIFNYASPYFEGDDPVQFCYQLVGYDKLWSAWTTSNRKEYTNL
                     PEGFYTFKVKAKNYMGIEGAPAEFRFRVKPPWYRTALAYFGYMIIAFLLIAGIIRAYT
                     YHLVLEKNKLEQLVAERTQEILQQKEEIQVQAEHL"
BASE COUNT         1009 a          773 c          902 g         1115 t
ORIGIN      
        1 ccccttgcta cttattacgc cccttcgggg cttaaaccac cagacaagtt ccgccagtaa
       61 gcggacaagt tccgccggta ggcggacaag ttctggaatt tgaaatccgc cggtaggcgg
      121 acaggttctg aaatctgaaa tccgccggta ggcggacaag ttccgaaatc cgtcggtagc
      181 cggacagtta ccgtaatttt aaattagcca attccgccca atacttcatt caacatttat
      241 tttttcattc ttaaacaggc ttaagattag tagctatcgt cccaagggat acctctacct
      301 aaactacctt tatgtatttc atttttatgt tgagttttct taacattttt ttaaattgcc
      361 acccaattat ttttcagaac ttactataga aagtgcagga tgagctataa gtgtgttttg
      421 gttgacgatg agccgctggc cgttggtgtc ctggaaggct tcttaaggca cattcctgac
      481 attgaagttg tagccacttt taacgaggca ataccacttt ttgacttcat tcagcacaac
      541 aaggttgaca tcatttttct cgatattgaa atgcccaacc tttcgggaat agacttccta
      601 aaatgcctaa aggatcctcc ttttgtcata attacctccg cgaaccgcga ttatgccgtt
      661 gatggttacg agcttaacgt tgttgactac attcttaaac cccttaccat ggagcgattg
      721 gtaagggcgg ttagtagagc tgctgaggcc ctggaattga aggctaggaa tgtaacggct
      781 gctccagatg cagagtatat ctttttaaag gagggtaaaa aattcattcg ggttaaagtt
      841 gatgaaattc aatacattga aagtattaag gattatgtga aagtggtagc tgaaggaaaa
      901 acagtagtta cacgaatgaa tattagtgcg gttgaaaagt tgcttgatcc tgccgagttt
      961 ataagggtgc atcggtcatt tattatttca aagaaacata ttgacgccta cacagcaacc
     1021 tctatcgaaa ttggttcggt tgaaattcct gttggaagga tttatcggga tgaagcagtc
     1081 aggcggcttg gctatgattt tgaagtttag caagctacca ggcgatggct tttgatgtac
     1141 cgcgtttggc attgccattt gttggcttta gccctacacc agagttcttc ccgcaaggca
     1201 ggggtgcgcc ttggggttta aatacctaga tttggggaga ggggaaccca taatgatgtt
     1261 gtcggggagc gaatccctgc tatcaacaca aaaaaaatgt tatgagtagc cgttttactt
     1321 tacactttaa cgttaccatg agcatttaca cccggtatat tctggcagtc gttttggcac
     1381 tcattggtta tcctaaaatg ccgcaagcgc aggagagagg cctgttttta tcgaggtact
     1441 ttccagcaag cgagtacaat ggctcaacgc agaattggtg tgctgttgag gacaacaggg
     1501 gggtgatgta ttttgcaaat ggtcagggcg ttctcgagta tgacggtgca acttggaatc
     1561 tgattagggt tgataacgaa tctgcaatac ggagcctggc agccgattcc tccggcagga
     1621 tatacgtggg agcctttgga gaaatgggat atctttaccc caaccggagt ggccagctgc
     1681 ggtatatgtc gctaatgtcc ttgctggata aaaatcattc gaggtttagc cagatttgga
     1741 atatctacac ttttggggat acagcatttt tcctttccga cagctgtctg tacaggtact
     1801 gtaatggcaa gttcagctac tggactagcc aggaaggttg cttctatttt gcttttaaat
     1861 tcaacaacca cctttacatt caggaaactg gagttgggct tcagcaattt gatgctgagt
     1921 ctcttgtgct tgttgaagag ggcaatctgc ttgcatcgca acggatacac gctattttac
     1981 cctttgggaa cgaaatgctg gtttgcacac ggagtaatgg cttgtttctc tttagaaatg
     2041 aaggggtaaa aaatcggctc accccagtct cgcacctatc gggcagagct ttagcagttg
     2101 accgttattt taaggagtac tcattctacc atggagtggc aataaacgat aatctcctcg
     2161 ccttgagctc cattaatggc aatttactta tggttgacac aacatggctg gttagggagg
     2221 ttattgatta caggacaaag ggagtaaaga gccccgttaa ctacctgtac ctccaaaaaa
     2281 acaacctgct ctggttggct ctcgacaatg gaatttgtgt ggttgagatt aattcgccat
     2341 ttcgctactg gactgatgac atgggggtta acggtgtggt taccgatgta gctaagcttg
     2401 gcggttttct gtatgtctca acccaagttg gaaatttttt tcttgatgaa aaaaaggaag
     2461 aaaattttat gcccgacatg ttcaaaaagg ttgaaggtga ttttgagcag tcgtgggaat
     2521 acctttactt taaggtttct agcaatttat tccctcaggg tcatagccgc ccagctgaca
     2581 cccttttgct tgtggcaacc cgaacgggtg tatatcgaat taatggcagc cggtcgagca
     2641 gaatatcaag gtatgaaccg gttttctgct ttcatcaaag taaagcaaat cctgctgctc
     2701 tcctggtggg cctttctagt gggatagcta ttgttgagta ccgtgatgga ttttgggtag
     2761 ataaagggtt tgtgcctggg attaaggaga atattgagca gatagccgag gatttagatg
     2821 gaaacgtatg gttgaaggct agctttagcg gagtttacag gcttaaaaac cttttctttg
     2881 gcagtagctt taagggtagc ttggtggttg aaaagtatga tacctcgagt ggtttgcccg
     2941 acctgaaatc cttatacatg actagccatg cagattcact ctttttttac tcccaaaacg
     3001 ggtactacac ctttaacgag aggttgaggc aatttcaagc gtacaatcca gcaaacaacc
     3061 ggcgattttc cgccgagaat gttgatactc tggcttggag aagggtatgg aataatatca
     3121 tttctggctt ctatgttgct cacacctccg attccatcat atggtttaac accccaaagg
     3181 ggttatttcg gtacactcca agcagctaca accgttgttt tgatgctcct gctactttta
     3241 tacgaaaggt agtaaacctg gattccacgc tattctatgg aaccaacttt gaaagttcga
     3301 aagtggacag tgctgccgga aaaccatttt ttttacctag ccataatccc cttgttgatc
     3361 tggggacagt gttggagtat aaacgtaact ctctgatatt caactacgca tcaccatatt
     3421 tcgagggaga cgaccccgtg cagttctgct atcagctagt tggttacgat aagctctggt
     3481 cggcttggac aaccagtaac cgcaaggaat ataccaacct accagagggc ttctacactt
     3541 ttaaagttaa ggctaaaaat tacatgggaa ttgaaggcgc tcctgccgaa tttcggttta
     3601 gagttaaacc tccttggtac cgaactgcct tggcatactt tggctatatg attatagctt
     3661 ttctactcat tgccggaatc attcgggcat atacatacca tctggttctt gaaaagaata
     3721 agctggagca gctggttgct gaaagaaccc aggaaatttt gcagcagaag gaggaaattc
     3781 aggtgcaggc cgaacacct
//
LOCUS       DAOOQX010000119         3796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_28748_length_3796_cov_2.770115, whole genome shotgun sequence.
ACCESSION   DAOOQX010000119 DAOOQX010000000
VERSION     DAOOQX010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28748_length_3796_cov_2.770115"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PLX53_02820"
     CDS             complement(<1..803)
                     /locus_tag="PLX53_02820"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOY72041.1"
                     /translation="MTKSSGILLAFLVVATVGCSNRSKLFPGFSVTPTGIHYKLISLG
                     NGTSTAKPSNYITASISYRTAKDSVFFQGTRQFQLAQSSYEGSIDECFMMLAEGDSGA
                     FYIKALPFFTQTLETKLPTFIGVNDYMRVDIRLHSIKTQEEFKKDMEAFLSWIEDFGD
                     YEKVIIRQYIDGQKLSVEPTPSGLYIIPQIRTERAPVEKGDTITIHYEGRFFDGRIFD
                     STRKRNEPFQLVYGDKWQVIPGIDEALGTMREGEKSMLIVPSHLAFGQQG"
     gene            complement(850..1374)
                     /locus_tag="PLX53_02825"
     CDS             complement(850..1374)
                     /locus_tag="PLX53_02825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOY72042.1"
                     /translation="MKILTIIPLAVILVSCGGNERQPDRQPNVAETREMLQRINKVLV
                     QQDREQILDYIKRNNLVEMKESPTGLFYYISGTSNGPKVRKGDVVEYSYRVSLLDGTV
                     CYQSNAEAPATFEVGHGGVVSGLEEGILLMRQGQSAKFIMPPHLAYGLIGDSKQVPAR
                     SIVIYDVELLAVYR"
     gene            complement(1409..2443)
                     /locus_tag="PLX53_02830"
     CDS             complement(1409..2443)
                     /locus_tag="PLX53_02830"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOY72043.1"
                     /translation="MKLNLDEAWAGALRELLVPTNRIVLTTHHNPDGDAVGSVLGLWH
                     ILEQVGVHATAVTPNDFPDFLSWMPGSDTILRFSSSRQKVREVMAGADIIICLDYNGF
                     SRTEDMESLFSASSAKKILIDHHPQPENTFDLSISVVEVSSTSELVFELATEIYGLKA
                     LNADAATCLYAGIMTDTGSFSYACSRGRTLEIAGMLVDSGAVVERIQSLVYNNFSADR
                     MKLLGYCLADKMMVFPEFHAAYIWLTREELKRFNYQMGDTEGLVNYPLSIKGIIFSVI
                     FVENDNHIKVSLRSRGNFSVNEFARKYYNGGGHLNAAGGKSFQPIDATLADFEQLLRQ
                     HLQELNAAAI"
     gene            2742..3161
                     /gene="ndk"
                     /locus_tag="PLX53_02835"
     CDS             2742..3161
                     /gene="ndk"
                     /locus_tag="PLX53_02835"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOY72044.1"
                     /translation="MNTRFTFCMIKPDAFKNDCVSEILCEILKAGFKVRGIKITMLTK
                     AKAEQFYDIHRGKDFFDKLTTYMSSGPVMAMVLEKPEAVEALRKLVGKTNPEEADEGT
                     IRRRFGVNTTQNAVHASDSYENAEHEWGFFFSKREIY"
     gene            3601..>3796
                     /locus_tag="PLX53_02840"
     CDS             3601..>3796
                     /locus_tag="PLX53_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72045.1"
                     /translation="MKKTLLSFLGVCLMAAAMVTNQAVAVPAYPGLIKYEQANGKIIN
                     IYLKGDEKVKWVLSEDGYTLM"
BASE COUNT         1130 a          878 c          795 g          993 t
ORIGIN      
        1 ccctgctgac caaaagcaag atgcgatggg acaattagca ttgatttctc gccctcgcgc
       61 atggttccca gcgcttcgtc aatacctgga attacctgcc acttgtcacc gtaaacaagc
      121 tgaaagggtt cgttacgctt gcgggtggag tcgaatattc gcccatcaaa aaagcgacct
      181 tcgtagtgaa tggtgatggt gtcccctttt tcaaccggag ccctttccgt tcgtatctgg
      241 ggaataatgt acaggcctga aggtgtaggc tcaactgata gcttctgtcc atcaatatac
      301 tgccttatga taaccttttc gtaatcgcca aaatcttcaa tccacgataa aaaggcctcc
      361 atatcctttt tgaactcctc ctgggtttta atgctgtgca aacgaatgtc aacccgcatg
      421 tagtcgttaa caccaataaa tgtgggcagc ttggtttcga gggtttgagt aaaaaacggc
      481 agtgccttta tgtaaaaagc accgctatcg ccctcagcca gcatcatgaa gcactcgtca
      541 atagaacctt cgtagcttga ctgggcaagc tggaactgcc gggttccttg aaagaaaacg
      601 gagtccttag cagtacggta ggaaatacta gcggtaatgt aattagaggg ctttgccgta
      661 gaagttccgt tgccaagcga aataagcttg tagtgaattc cggtgggggt tacagaaaat
      721 ccaggaaaaa gtttcgaccg gttggagcag ccaaccgtag ccacaaccag aaatgctaac
      781 agaatacctg aactttttgt catgcagcta aggaattaat gcgtgtaaag ttagccaaaa
      841 tatcaaaaat caacgataaa cagccaacaa ttcaacatcg taaataacta ttgacctagc
      901 gggtacttgt ttgctatcgc caatcaggcc gtaggccaaa tgggggggca tgataaattt
      961 agcgctttgt ccctgcctca tcagcagaat gccttcttct aaacctgaaa caacaccccc
     1021 atggccaacc tcaaaggtgg ctggggcttc tgcattggat tggtagcaaa cggttccatc
     1081 gagcagcgaa accctgtagc tatactcaac aacatcgccc tttctaacct tggggccatt
     1141 agatgtccct gaaatatagt aaaataaacc tgtagggctt tccttcatct ccaccaagtt
     1201 gttccgctta atgtagtcca gtatttgctc cctatcctgc tgaaccaaca ccttgttgat
     1261 acgctgaagc atctccctag tttcagccac attgggctgc ctgtcgggct ggcgctcatt
     1321 tcctccacac gataccagga taacggctaa agggatgatg gttagtattt tcatcagcaa
     1381 tctatctcaa cgtcttcttc acctatattt atattgctgc agcattcaac tcctgtaaat
     1441 gctggcggag aagctgctcg aaatcagcca atgtagcgtc aataggctga aatgatttcc
     1501 ctccggcagc attaagatgg ccgcctccgt tgtagtactt acgggcaaac tcgttaacag
     1561 aaaaattacc cctcgaccga agcgaaacct taatatggtt atcgttctcg acgaatatca
     1621 ccgaaaaaat tatccccttg atggaaaggg ggtagttgac taacccctcg gtatcgccca
     1681 tttgatagtt aaaccgcttg agttcctccc tggtcaacca gatatacgca gcatgaaact
     1741 cagggaaaac catcatttta tctgccaggc agtaacccag cagcttcatt ctatcggccg
     1801 aaaagttatt gtaaaccaac gattgaatgc gctcaacaac ggctccactg tcaaccagca
     1861 ttccggcaat ttcgagggtt ctaccccgag aacaggcgta gctaaacgat ccggtatcgg
     1921 tcattatgcc tgcatacagg caggtagctg catcggcgtt aagtgctttg agcccgtaaa
     1981 tttcagtagc cagctcgaaa accagctcgg aggtggagct cacttccaca accgaaatgg
     2041 ataaatcgaa ggtattttcc ggttggggat ggtggtcaat aagaattttt ttggctgaag
     2101 aggccgaaaa caaggattcc atatcttctg tccggctaaa cccattgtaa tcaagacaaa
     2161 ttataatatc ggcaccagcc atcacctccc ttaccttttg cctactgctg gaaaatcgca
     2221 gaatcgtatc gcttcccggc atccatgaaa gaaaatcggg gaaatcgtta ggggtaacgg
     2281 cagtggcatg aaccccaacc tgctcaagta tatgccatag cccaagcaca gagccaacag
     2341 catcaccatc agggttatga tgggttgtta gcactattcg gttggtagga accagcaact
     2401 cccttaatgc ccctgcccat gcctcatcaa gattcaactt catcgagctt ttattttact
     2461 atgatgcacc catgtttttg gagccacaag ctccattgta aaataaccaa catgggtgtt
     2521 tcaccatacc tttaaaatca aattttatta tgatgaaatt tacaaagata gattttgccg
     2581 acaagaaagt tttgcctatt tttaaaaaat tccggtaaag aggggtgccg cttcaccgga
     2641 agtattttaa acatgaattt caaaatggaa gcatttgccc gctagagctg ctgttgtatt
     2701 aatgccagac atgttaaaca ggttaacaaa aatcttaagg tatgaacacc cgattcacat
     2761 tttgcatgat caaacccgat gcttttaaga acgactgcgt aagcgaaata ctttgtgaaa
     2821 ttcttaaagc cggctttaaa gtaaggggca taaaaattac catgctaacc aaggctaagg
     2881 ccgagcaatt ctacgacatc caccggggta aggatttttt tgacaagctt acaacctaca
     2941 tgagttcagg ccctgtgatg gcaatggttt tagaaaaacc tgaagccgtt gaagcgcttc
     3001 gaaagctggt gggaaaaacc aacccggagg aagccgacga gggaacaatt cgcagacgat
     3061 ttggtgtgaa taccactcaa aatgccgtac atgcctctga cagctacgag aatgccgagc
     3121 atgaatgggg attcttcttt tcgaaacggg aaatctacta gacaaaagaa ttttcttacc
     3181 ctgacctagc gctcgtcatc tcgtatattt aaaatgatgg gaaaaatagc tgtaaaagaa
     3241 aatcagcatt attgagccca ttttccacta tggagactac ctataatgca ggtagataaa
     3301 gaaatcatct tatagccaac aacaacccta gcttacaaca aaattgctcg cccctatccc
     3361 taagaaagat tagcaggtaa cttatcccac aaatccgtta ttattaaaaa gttatagaat
     3421 cataacgact tataatgctg atattaagcc attttgtcgt ttgtgcaaag gaacatgtag
     3481 tttgtactta gaaagctgct ttatgtcgca aaataaaatt ttgcattatt cagcaactta
     3541 aattacgtcc cgttaagtaa accttttttt taaccaacct aaaattaatt ctattaagct
     3601 atgaagaaaa ctttactaag tttcttgggt gtatgcttaa tggcagctgc aatggtaacc
     3661 aaccaagccg ttgctgtgcc ggcataccct ggcctgataa agtacgagca ggccaacgga
     3721 aagatcatca acatctattt aaagggtgat gaaaaagtta agtgggtgct aagcgaggat
     3781 ggctacaccc ttatgc
//
LOCUS       DAOOQX010000120         3789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_28820_length_3789_cov_3.335833, whole genome shotgun sequence.
ACCESSION   DAOOQX010000120 DAOOQX010000000
VERSION     DAOOQX010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28820_length_3789_cov_3.335833"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1374
                     /locus_tag="PLX53_02845"
     CDS             211..1374
                     /locus_tag="PLX53_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOY72046.1"
                     /translation="MSGIGEQWVYVGGWNGLGSDITDSFKVSTRGRVNFSLQTTTPEM
                     LALSFDRVHFPILLCVEPGSNVEVTGTASSYQVKGSFESARINTFQQRYNSFLSEVAH
                     LSGLIPDSTATPESDSLASVLIHKRDSLISSFRTYTLSEVRANPYCLSSVPFILTTFD
                     GGQELLPYELFGNTYQKVDSCLGLAYPDKPLVQTFHQHIQMQEKKLAQLHKADRYSTG
                     DVLPAISFETVDSQAISIPGLWAKAILIEFWADWCPQSVSTADQLRILYEKHSNQGFK
                     IIRVGMGLEPEALYQRVKRDSIPWINVAIPDSEKDYWVDSIGVVYLPFNFLIDRSGRV
                     VAKNLFGDTLVRKLDDLLGANSGRLKPDVHPKTGITSKPEVKVNTITSNPETK"
     gene            1382..2167
                     /gene="rsmA"
                     /locus_tag="PLX53_02850"
     CDS             1382..2167
                     /gene="rsmA"
                     /locus_tag="PLX53_02850"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOY72047.1"
                     /translation="MPVVRPKKSLGQHFLHDRRIAGLIVSSLQAEGVDYVVEVGPGMG
                     VLTEDLLQRFGPVFYAVEVDGESVQYLRQRLPELGGNLIEADFLDVDIENCFNGRVAI
                     IGNLPYNISSQIFFKILAMRQRVVEAVVMVQREVAQRIAEKPGSRTYGILSVLLQAYF
                     NIEYLHTVSEGAFTPPPRVKSSVIRLTRNHVAELDCNPELFVRVVKASFNQRRKMLRN
                     AIHSAFPGVEAGKPFSERRAEQLSVADFVELTNMVEQQLTAVS"
     gene            complement(2169..3434)
                     /locus_tag="PLX53_02855"
     CDS             complement(2169..3434)
                     /locus_tag="PLX53_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY72048.1"
                     /translation="MNALKPFIILIRLARESMQFAYQSIKANKLRTFLSLFGITIGIF
                     AIISVFTMVDSLEKNIRNSLESLGNNVVYVQKWPWGGNEEYPWWKYLNRPEPTLNDMS
                     EIRRRSSLAQASCFNVSFNKQVKFQNKSLSNVTIYGTTNGYDEIRSFELGSGRFFNLF
                     ELNSGKPYAVIGATICEELFQNADPIGQSIQIGGKKATVIGVFAKEGKSAIGETSSDD
                     MVVIPLGMAKSMVDLRNSGPWIMVKARDNVVPEDLIDELRGILRSYHRLKPLDSDDFS
                     LNQIDMMKESLDAIFSSISFAGGFIAFFSIIVGGFGIANIMFVSVKERTSQIGIQKAL
                     GAKRYFILIQFLYEAMLLAILGGIIGLILIFAGTLIVTYGLDFTISLTLENIIWGLAI
                     SAIIGIASGFIPAWMASRLSPVEAINTKS"
BASE COUNT          991 a          840 c          881 g         1077 t
ORIGIN      
        1 taaatttaca aatgatatat taaaacaatg taatttttga atttatcgaa aatttcgttt
       61 gcacccagtg ttaattttta taaaagtttt atgcaccctt caaaatatta ccattccaca
      121 acttttttta ggttcctttt tgtcctgcca gctgcaattg caattagctc ctgcactaat
      181 tcctataaac gagcaagcat tagccttaaa ttgagtggca taggagagca atgggtttat
      241 gttgggggat ggaacggatt aggcagcgat ataaccgatt cttttaaggt aagcacacgc
      301 ggaagggtta acttttctct acaaaccaca acacccgaaa tgcttgcact ttcgttcgac
      361 agggttcatt ttccaatcct gttatgtgtt gaaccaggtt caaatgtgga ggtaaccggt
      421 actgcaagtt cataccaggt gaagggttca tttgaaagcg ccaggataaa cacttttcag
      481 caacgctata atagtttttt atctgaggta gctcatctca gcggcttaat tccagacagc
      541 acagccactc ccgagagcga ctcgttagca tcggtactta ttcataaaag agactcactt
      601 atttccagct ttaggacata caccctttca gaggtgaggg ctaaccccta ctgcctgtcc
      661 tcagttccct tcattttaac tacttttgac ggagggcagg agcttctacc ttacgaactc
      721 ttcggcaata cttaccagaa agttgattcc tgcttgggct tagcatatcc cgataaacct
      781 ctggtgcaaa cttttcacca gcatattcaa atgcaggaaa aaaaacttgc acaattgcat
      841 aaggctgata ggtattccac tggagatgtt ttgccagcta taagttttga aactgttgat
      901 agccaggcga tatctattcc cggactttgg gctaaagcta ttcttattga gttttgggct
      961 gactggtgcc cacagtcggt gagcacagcc gaccagctgc gcatactcta cgaaaagcac
     1021 tccaaccaag ggttcaaaat cattcgtgtt ggtatggggt tagagcctga ggctctctac
     1081 cagagggtta agagggactc catcccgtgg ataaatgtgg ccatacccga ctcggagaaa
     1141 gactactggg ttgattcgat tggtgtcgtt taccttccat tcaacttcct tatcgatcga
     1201 tccggtaggg tggtggctaa aaatttattt ggcgataccc ttgtgcgcaa gctggatgat
     1261 ttgctcggcg cgaattctgg tcggctaaag cccgatgttc atccaaaaac cgggatcact
     1321 tctaagcctg aggtaaaggt aaacacaatt actagcaatc ccgaaactaa atagcaagtt
     1381 aatgcctgtt gttcggccta aaaagagttt ggggcagcat ttcctacacg atcgtaggat
     1441 tgcagggttg attgtttcga gccttcaggc ggaaggtgtt gactacgttg ttgaggtagg
     1501 gccgggtatg ggcgtgctta ctgaggactt gctccagcga ttcggccctg ttttttatgc
     1561 ggtggaggtt gatggcgagt cggtgcagta ccttaggcag cgcttaccgg agttgggcgg
     1621 caatcttata gaagccgatt ttctggatgt tgatattgaa aattgcttca atggacgagt
     1681 tgccattatc ggtaacctgc cctacaacat ttcgtcccaa atatttttta aaattcttgc
     1741 catgcgccaa agggtggtgg aggcagtggt gatggtgcaa cgcgaggtgg cgcaacgcat
     1801 tgccgaaaag cccggttcca gaacctacgg cattcttagc gtgctgcttc aggcatactt
     1861 caacattgag tatttgcata cagtgagcga gggcgcgttt accccaccac ccagggtgaa
     1921 atcgtcggtc atcaggctaa cccgtaacca tgtggcagag ctagactgca acccggaact
     1981 ctttgtacgg gtagttaaag cttcgtttaa ccaacgtcga aagatgctgc gaaatgcgat
     2041 tcactcagcg tttcccgggg ttgaagctgg taagcccttt tcggagagga gggctgaaca
     2101 gctttcggta gccgattttg ttgagttgac caacatggtg gagcagcagc tcacagcggt
     2161 gagttagcct acgattttgt gttgattgcc tcaaccggac ttaacctgga ggccatccac
     2221 gcagggataa aaccagatgc aattcctata atagccgaaa tggcaagccc ccagatgatg
     2281 ttctcgagtg taagcgatat ggtaaaatca agcccgtagg taactatcag cgttccagca
     2341 aatataagga ttagtccaat aatgccaccc agaattgcca gaagcatggc ctcgtagagg
     2401 aattggatga gtatgaagta gcgctttgcc ccaagcgcct tttgaatgcc aatctggctg
     2461 gttcgctcct taaccgaaac aaacatgatg ttggcaattc caaatccacc aacaattatt
     2521 gagaagaagg ctataaaccc accagcaaag ctaatggagc tgaaaattgc atcgaggctc
     2581 tccttcatca tgtctatctg gtttagagag aagtcgtcgc tgtcaagcgg ttttaggcga
     2641 tggtatgacc ggagtattcc cctcaactcg tcaatcaagt cttcggggac aacgttatcg
     2701 cgagctttga ccattatcca tgggccagaa tttcgcaggt caaccataga cttggccatg
     2761 cctagaggga ttaccaccat atcgtctgag ctggtttcgc caatggccga tttgccttcc
     2821 ttggcaaaaa cgcctataac cgtggctttt ttcccaccaa tttgtatgct ttgaccaata
     2881 ggatcagcat tttggaatag ctcctcacaa attgtagcac ctatcacagc gtatggcttg
     2941 ccggagttta gctcgaaaag gttgaaaaac ctgccgctgc caagctcaaa cgatcttatc
     3001 tcgtcgtatc cgttggtagt cccgtaaata gttacgtttg acaggctttt gttctgaaac
     3061 ttgacttgct tgttaaacga cacgttgaaa cagctggctt gagccaggct actccggcga
     3121 cgaatttcgc tcatgtcatt tagtgtgggc tctggtcggt ttagatactt ccaccagggg
     3181 tattcctcat tgccacccca cggccacttt tgcacgtaaa ccacgttatt tcctaacgat
     3241 tccaagctgt tccggatatt tttttccaga ctgtcaacca tagtaaatac cgagatgatg
     3301 gcaaaaattc caatggttat gccaaacagc gagaggaatg tccgtagttt gttggccttt
     3361 atggactggt aggcaaactg catgctttcc cgggcaagcc tgatgagtat gatgaagggt
     3421 tttagggcat tcattctgat acatgttaaa caaagcaaag gtatttaaaa aattatggtc
     3481 tagccaaatg gatttgttaa ttactctgaa tgcgctggtg gaaacagaat ttttgaagga
     3541 gtatatttgt ttaaccacta tgaattctgg cgtgcacttg cttttaataa ttttatccgt
     3601 acaaaatgtt taggttttct attacaaaaa ccttggccca taccttttaa gtttggggtt
     3661 gaggggatgg atctcccata ttttggcttc gccgaactcg caactaacaa ccgattgttg
     3721 atgcgtgctc acgctggctg cacaacatgg gtgttttata aagttttaca acttactttt
     3781 tttattact
//
LOCUS       DAOOQX010000121         3781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_28909_length_3781_cov_3.086957, whole genome shotgun sequence.
ACCESSION   DAOOQX010000121 DAOOQX010000000
VERSION     DAOOQX010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28909_length_3781_cov_3.086957"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            752..1249
                     /gene="folK"
                     /locus_tag="PLX53_02860"
     CDS             752..1249
                     /gene="folK"
                     /locus_tag="PLX53_02860"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOY72049.1"
                     /translation="MARVYFTIGGNLGAREKNLEEARNLISARIGTVSKQSAIFESEP
                     WGFSSNDNFLGQVLMVETSLTPAALMLEISYIESKLGREHQGIMYSSRTIDIDVLFYD
                     QLVLFTPELIVPHKHLHKRRFVLEPLNEIAPNFIHPLFSMTVHELLSSCPDECKVWRY
                     QPVGA"
     gene            1242..1364
                     /locus_tag="PLX53_02865"
     CDS             1242..1364
                     /locus_tag="PLX53_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72050.1"
                     /translation="MPSYKHNKLKLGFKLVVAVITKVKNPFTLIAIVGAFLRFE"
     gene            complement(1525..2148)
                     /locus_tag="PLX53_02870"
     CDS             complement(1525..2148)
                     /locus_tag="PLX53_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72051.1"
                     /translation="MKLTHNSLRLMVLLSSFVIVGNANALSLGSSDTCKVDYLSFQSG
                     MIIDRYNSLGVRIFFEYQRDIKKKWQWGISYENSRHLGFAATDQPYELETNLNLFSLN
                     GYYKLNVVKNRLFWTAGLGLGMVHAYWEKPLSYTFSEMEHHNIFGPVANVSLTLNVRI
                     TKRVYIEASPLVVVMPTNRVYFSTMDEEHFNNFFAFTFFPFGVKFRL"
     gene            2440..>3781
                     /locus_tag="PLX53_02875"
     CDS             2440..>3781
                     /locus_tag="PLX53_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72052.1"
                     /translation="MKKVGILITVAALVVGTIAVVVYNWLSNERKKDIEATAALPADA
                     PLVVKIDSYTQISQSLKASNEWKSLLTREDVALVDRMVAITDSMVSVNPVAAGLLKPS
                     AIYAAILPQGDKSNQLLVSLKVPENISISDIRQLIEPLAFGSYTSHVKTYNRTEMLVL
                     TSTSNPQKQAALAIKHGILMVGSREIMVEETINRLESEYTLQSNQKFATINQMAGQGV
                     DASLFINFSKLPVLLTSWLNDNFQANANFVADVGDWGEVDLQLTKSRLTTSGIITTSD
                     SATSFLGLIADQKPVQPEIPGILPAQTSFYFWLGISDLGRYLEDYRTYLDRSGRLFAY
                     TQALAKIGGKVAMEPEELYKRILLNELCLVCLPSTPSGNVDEWYVVAQNQSSSTTGHL
                     LGSLSGKGGSLPAASINVDDKHSYGVFRSPVPGIHRALFGDLFSVVSDEYYCLID"
BASE COUNT         1087 a          828 c          792 g         1074 t
ORIGIN      
        1 agcattgtgc ccaactcatt tatacacgcc actctgttgc gccttatttc catatttgga
       61 tggatagacg ccataaagtt gcgttctatt tctattcaaa agtagaaaaa ttatttattt
      121 gataaaccaa acttttgaac ttccttacta caaaccatca aaaggcggtt taattttttg
      181 tagttttttt cagtaacgtg catgtaaatt tctgtggttt tagaatgttt gtgcccgaaa
      241 agtttctgaa taaagtttaa atctattccc tcgcttggta taggtcacct tcggcgagct
      301 ctcccctagc tggcggatcg gtcagcgtag cgtaaccctc gattggtgtt actttgggcg
      361 cctgtatttg agcatggggt tgctacatcg ttgcaaaaga gcagcccttt aacagccttg
      421 cagtaaggga taatttgctg tttagttgca ggcaggaggc catacttagc tacggctatg
      481 tcgtaaactt tgccggtaaa aggttaaatt gaccataacc aacttttaag gcagtagatg
      541 tgttaggtga acattacacc gaatttgcct gtttaacctt tgcagctggt attctttttg
      601 ctgttgtaat aatgggttct tgtaaattaa aaatatagct attcgacgaa aaccaaacat
      661 accattaaaa ttgttcgtaa aaattaaccg gtctaagaac attttagtaa aatttcaaat
      721 aagtttgtac catataaact ggtaaaggga aatggcaagg gtttacttca caattggcgg
      781 gaatctaggt gccagggaga aaaatttgga agaagcacgc aaccttattt ctgcacgaat
      841 cgggacagtt agcaagcaat cggcaatttt tgagtctgaa ccttggggtt tctcaagcaa
      901 cgataatttt ctagggcaag tgctcatggt cgaaaccagc ttaaccccag ctgcgctcat
      961 gctcgagata tcctacatag agtcgaagct gggacgtgag catcagggaa tcatgtattc
     1021 ctccagaaca atagatattg atgtcctttt ctacgaccag cttgtgcttt tcacacctga
     1081 acttattgtt ccccacaagc acctgcacaa gcgtcgcttt gtgcttgaac cgctcaacga
     1141 gattgctccg aatttcattc atccgctctt ttccatgaca gtgcacgagc ttctttcatc
     1201 ctgccccgac gaatgcaagg tctggcgcta ccaacctgta ggtgcctagc tacaagcata
     1261 ataagttaaa gttagggttt aagctggttg tagcagtaat taccaaggta aaaaatccct
     1321 ttacgttaat cgccattgtt ggcgcattcc tccgttttga ataacctttg gtgttggccc
     1381 aagaggtaaa accatatttt aaactttcca ccccgaacta ttcattttgt cagctttgag
     1441 gcagctgccc aacctggttt ctgtttacgg ttggagatac cctgacctgc ccttttcaac
     1501 cttatgacat tattaaaagt ttctctatag cctgaacttt accccaaagg gaaaaaatgt
     1561 aaaggcaaaa aagttgttga aatgctcctc gtccattgtc gaaaagtaaa ccctattggt
     1621 aggcattaca acaaccagag gggacgcttc gatatatact ctctttgtaa tcctaacatt
     1681 cagcgtaaga cttacattgg caaccggtcc aaaaatatta tgatgctcca tttcgctaaa
     1741 tgtatagctt agtggctttt cccagtatgc atgaaccatt ccaagcccaa gccctgctgt
     1801 ccagaaaagc ctgtttttga caacgttcag cttatagtag ccgttcaggc tgaaaaggtt
     1861 gaggtttgtt tccaactcgt aaggttgatc ggttgcggca aaaccaaggt gcctggagtt
     1921 ctcgtatgaa attccccatt gccatttttt tttaatatca cgttggtact caaaaaaaat
     1981 ccttaccccc agcgaattgt agcggtcaat tatcattcca gactgaaagg ataaatagtc
     2041 aaccttacag gtgtcgctgc tcccaagaga taaagcgtta gcgttgccaa ctatcacaaa
     2101 tgatgagagc agcaccatca gccttaaact attatgcgtc aacttcatat ggcatcatcg
     2161 tgtaagttaa aaaaatcaat caaccggata agttttgctc ttgctgctac aaattagtat
     2221 aatccgcaac aggtataaat gtaagaaaaa tgttcatgca gctaattcca tgtaataaga
     2281 aatccagctg ggacgagggg ttaacaggat tgagtaaatc agccgcagca aaatgagctg
     2341 cattttctaa gaaaccaagt atctttttac gattaatcct gactaattta gcacctttat
     2401 gttaccatta gtagctacat taaaaaaagc agctgacaga tgaaaaaggt tggcatttta
     2461 attacagttg cggcgttagt ggttggaaca attgccgttg tagtttataa ttggctgtcg
     2521 aacgagcgta aaaaagatat agaggccacc gctgctttac ctgcggatgc ccctctagtt
     2581 gtaaagattg acagctacac gcagattagc caatcgctta aggcaagcaa tgaatggaag
     2641 tcgctactga caagagagga tgttgccctt gttgatagga tggtggccat taccgacagc
     2701 atggttagtg ttaacccggt tgcagcaggt ctgctaaaac caagcgcgat ttatgcagcc
     2761 atcctgcctc aaggcgacaa gtcaaatcag cttcttgttt cattaaaagt acccgaaaac
     2821 atctctataa gcgacatacg ccaacttata gagccattgg cgtttggcag ctataccagc
     2881 catgttaaaa cctacaatag gaccgaaatg ctggtattaa catcaacctc gaatccacag
     2941 aagcaagcgg ctttagcaat caagcacggc atcctaatgg ttggcagccg ggaaatcatg
     3001 gttgaagaaa cgattaaccg gcttgaaagc gaatatacgc tacaaagcaa ccaaaaattt
     3061 gctactataa accaaatggc cggacagggg gtagatgcct ccctttttat caacttttca
     3121 aaattacctg ttctacttac atcctggctg aacgacaatt ttcaggcaaa tgcaaatttt
     3181 gttgccgatg ttggggattg gggtgaggtt gacctccagc ttacaaaaag taggctaaca
     3241 accagtggta taatcacaac atccgactct gcaacctcat ttctgggact aatagccgat
     3301 caaaaacccg ttcagcccga aattccgggt attctacccg cacaaaccag cttctacttc
     3361 tggctgggca tttcagacct tggtaggtat cttgaggatt accgcaccta ccttgaccga
     3421 tcaggaaggc tgtttgcata tacgcaggca cttgccaaaa ttggtggtaa agttgcgatg
     3481 gaacccgaag agctatacaa gcgtattctg ctcaatgaac tttgtttggt ttgcctcccc
     3541 tctactccat ccggcaatgt tgatgaatgg tatgttgtag cacaaaacca aagctccagc
     3601 acaacagggc acctgcttgg cagtctttcg gggaaaggcg gctcactgcc tgctgcgagc
     3661 attaatgttg acgacaagca ttcgtatgga gttttccggt ctcccgtgcc tggaatacac
     3721 cgggctctat ttggagacct ttttagtgtt gtatcggatg aatactactg cttaatagac
     3781 c
//
LOCUS       DAOOQX010000122         3770 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_29017_length_3770_cov_5.570659, whole genome shotgun sequence.
ACCESSION   DAOOQX010000122 DAOOQX010000000
VERSION     DAOOQX010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29017_length_3770_cov_5.570659"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..801
                     /locus_tag="PLX53_02880"
     CDS             130..801
                     /locus_tag="PLX53_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOY72053.1"
                     /translation="MMGIFSSSIGKKLIMSITGIFLMIFLLVHLSVNLTLLVGDGETF
                     NHAAHFMGTNPLVKIIEPILAAGFILHIFFATYITLKNRGTRPVGYTQTSRNGVSTWP
                     SKNMYILGGLILVFLVIHLSNFFWKMRYGEMPTITYNGLVMDDAYSLVADLFIKYWWY
                     DLLYLAAAVLLGLHLSHGFWAAFQTVGWNGTKWLKRLEVIAYIYAVIIGIGFSVIPLY
                     FLIFK"
     gene            824..2743
                     /locus_tag="PLX53_02885"
     CDS             824..2743
                     /locus_tag="PLX53_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOY72054.1"
                     /translation="MTKLDAKIPEGPLADKWTNHKAKLRLVNPANRKKIDIIVVGTGL
                     AGSSAASTLGELGYNVKVFCYQDSPRRAHSVAAQGGINAAKNYKNDNDSVYRLFYDMI
                     KGGDYRAREANVYRAAEVSNLVIDHYTALGVPFARDYGGHIDNRSFGGVQVSRTFYAK
                     GQSGQQCLIGAYSDLSRQISMGTVTMYPRREMMDLVLVDGKARGIVVRNLITGEIERH
                     SAHAVVLATGGYGTIYYLSTLALNSNGSAAWKAYKKGAYFANPSFVQIHPTSIPVLND
                     YQCKLTLMSESLRNDGRVWVPKQKGDTRKPNDIPEEDRDYYLERRYPAFGNLVPRDVA
                     SRAAKERCDAGYGVGPTGLSVYLDFRDAISRKGKKYIEDNYGNLFEMYEKISGESPYS
                     VPMRIYPAGHYTMGGLWVDYNLMTTVPGLFAIGESNFSDHGANRLGASSLMQTSGDGY
                     FILPYTISDYLADEIRTPKISINIPEFDEAENTVRERINKLLGINGTETSTSFHKRLG
                     KIMWDYCGMVRNEEGLKKALALIDELKDEFWHNLKLPDSSNEVNHELEKACRVADFLE
                     LSKLLVYDALNRRESCGAHFREESQTETGEALRDDEHFSYVAAWEFAGDSKEPILHKE
                     ELKFEYVKLQERSYK"
     gene            2777..3520
                     /locus_tag="PLX53_02890"
     CDS             2777..3520
                     /locus_tag="PLX53_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOY72055.1"
                     /translation="MNIKLKIWRQKNAQSKGGFETYLLDGVSPEMSFLEMLDYLNSRL
                     IIEGKSPVAFDFDCREGICGSCGIYINGRPHGSDRKTTTCELRMRKFKDGETITLEPW
                     RAGPFPVIKDLMVDRSAFDKIIQAGGYITVNAGAAPEANSILVPKKNADTAFDTASCI
                     GCGACVAACKNASAMLFVAAKITHLAQFPQGRIEAPERVKRMVAKMEELGFGGCTNTR
                     ACEAECPKGISITAIAKLNRDYISAKMKD"
BASE COUNT         1028 a          779 c          913 g         1050 t
ORIGIN      
        1 cgctggccat tgggaagggt aaatcggtga tatctgaaat ttcaaattta taagtttctg
       61 cgtacggcca tgtattttat tatttaagaa ctttttctaa cttgttcacc aaaattaaac
      121 tttaatgcca tgatgggaat tttcagctcg tccattggga aaaagttgat aatgagtatt
      181 acagggattt tcctgatgat attcctgctt gttcacctct ccgtaaacct gacgctactt
      241 gtaggagacg gagaaacatt taaccatgct gcccacttta tgggcaccaa ccctttagtc
      301 aaaattatcg agccaattct ggccgcaggt tttattctcc acatcttctt tgctacttac
      361 atcacattaa agaacagggg cacccgtccg gttggttata cccaaacttc aaggaatgga
      421 gtttccacat ggccatcaaa aaatatgtac attttgggtg ggttgatttt agtattcctg
      481 gttattcacc tttccaactt tttctggaag atgcgatatg gcgaaatgcc cacaattacc
      541 tataacggtc tggtaatgga tgatgcttac tcccttgtgg ccgatctttt tataaaatac
      601 tggtggtacg acttgcttta cttagcggca gcagtactcc tagggttgca ccttagccac
      661 ggtttttggg ctgctttcca aaccgtaggc tggaatggaa ctaaatggct taagcggtta
      721 gaggtgattg cctatatcta tgcggttata atcggtatag gttttagcgt aattcctctc
      781 tacttcttaa ttttcaagta atcacagcta acatatacaa tatatgacta agctcgatgc
      841 aaaaatcccc gaaggtccat tggccgataa atggacaaac cataaggcaa agttgcgcct
      901 tgtgaacccc gcaaaccgta aaaagattga tattattgtt gttggaactg gtttggctgg
      961 ctcgtcggca gcatcaactc tgggcgagtt gggctacaac gttaaagttt tctgctatca
     1021 ggatagccca cgcagagcac actctgtggc tgctcagggt ggtataaacg ctgcaaaaaa
     1081 ttacaagaac gataacgata gcgtttatcg tcttttctac gatatgatta agggaggcga
     1141 ttaccgtgcc cgcgaggcca atgtttaccg tgccgcagag gttagtaacc tggtaattga
     1201 ccattatact gcgctgggag ttccgtttgc aagggactat ggtggacata tagataatcg
     1261 ttcatttggg ggcgtccagg tgtccagaac attctacgcc aaagggcaaa gtggtcaaca
     1321 atgtttgatt ggcgcttact ctgacctatc gcggcagatt agcatgggca cagttacaat
     1381 gtatcctcgc cgcgagatga tggatttggt tttagttgat ggcaaggcgc gaggtattgt
     1441 tgttcgcaat cttatcaccg gcgaaattga gcgccattcc gctcatgccg ttgttttggc
     1501 caccggtgga tatggcacaa tctactacct atcaacatta gcgctaaatt caaatggttc
     1561 tgccgcttgg aaagcttaca aaaagggtgc gtattttgct aaccccagtt ttgttcaaat
     1621 tcatccaacc agcattcctg tgctgaacga ctaccagtgc aagctaacgt taatgtcgga
     1681 atcgctccgc aacgatggta gagtttgggt tccgaagcaa aaaggcgaca cccgcaaacc
     1741 taacgatatt cctgaggaag atagggatta ctacctggaa cgtcggtatc cggcttttgg
     1801 caatctggtt cctagggatg tagcttcgcg tgccgcaaag gagcgctgcg atgcggggta
     1861 tggtgttggt cctaccggtt tgtctgttta ccttgatttt cgtgatgcca ttagcaggaa
     1921 agggaagaag tacatcgagg ataactatgg taacctcttc gaaatgtatg aaaaaatttc
     1981 aggagagtcg ccatactctg ttcccatgcg aatttacccg gcaggccact acaccatggg
     2041 ggggctttgg gttgattata acctgatgac aaccgtgccc ggcttgttcg ccattggcga
     2101 atccaacttt tccgatcacg gagcaaaccg cctcggtgca agttccttga tgcagacctc
     2161 tggcgacggg tactttattc ttccttatac catttccgac taccttgccg atgaaattcg
     2221 tactcccaaa atttcaatta acattcctga gttcgacgag gcggaaaata ctgttcgtga
     2281 acgcattaac aagctgcttg gtataaatgg aaccgaaaca tccacttcgt tccataagcg
     2341 cctgggtaag attatgtggg actactgtgg aatggttcgt aatgaggagg gtttaaaaaa
     2401 agccttagca ttaattgatg agctaaaaga tgaattttgg cataacctta aactacctga
     2461 ttcttccaat gaggtcaacc acgaacttga aaaggcatgt agggtggccg attttctgga
     2521 gttgtcaaaa ctgctagttt acgatgcgct taaccgtagg gaaagttgtg gtgcacactt
     2581 cagggaggaa agtcaaaccg aaacgggcga ggccttgcgc gatgatgagc atttttcgta
     2641 tgtcgctgct tgggagttcg cgggcgacag taaagaacca attctccaca aggaggagtt
     2701 gaaattcgag tacgttaaac ttcaggaacg aagctataag tagtaaatat ttacgttgtt
     2761 ttaactatta agacaaatga atatcaagct taaaatatgg aggcaaaaaa atgcccaaag
     2821 caagggaggt tttgaaacgt acctgcttga cggtgtttct cctgaaatgt catttttgga
     2881 gatgttagac tacttgaata gccgcttaat tatagagggt aaaagccctg ttgcattcga
     2941 ttttgactgt agggagggca tttgtggttc ttgcggaata tatattaacg gaaggcccca
     3001 cgggagcgac agaaaaacca caacttgcga gttgcgtatg cgaaaattca aggatggtga
     3061 aacaataacc cttgaacctt ggcgtgcagg accttttccg gttatcaaag acctgatggt
     3121 tgacaggtca gcattcgaca aaattattca ggccggcggg tatataaccg ttaacgctgg
     3181 tgctgcgcca gaggcaaaca gcatactagt tcccaaaaag aacgcagata ctgcttttga
     3241 taccgcctca tgtattggat gcggggcttg tgttgcagcc tgtaagaatg cttcagccat
     3301 gctgtttgtt gctgctaaga ttactcattt agcccagttt cctcagggta ggattgaggc
     3361 tcctgaaagg gtaaagcgaa tggttgcaaa aatggaagag ctgggctttg gcggctgcac
     3421 aaatactcgt gcatgtgaag cagaatgccc caaaggaatc tctattactg ccattgccaa
     3481 gctaaacagg gattacattt cagccaaaat gaaagattga ttttattggt attttgggct
     3541 tatggtaaac tattaagaaa aaataggttg atgaaatgcg ataaaaaatt acctttgcgc
     3601 agaatttcag taattattaa gacaaattat caggctttaa actttcatat ttgcgggatt
     3661 agctcagctg gtagtccgcc agccggcgga tcgcaagctg aggagcgcag ttcgttgcgg
     3721 gagtagctca gacggtagtc cgccagccgg cggatcgcaa gctgaggagc
//
LOCUS       DAOOQX010000123         3755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_29158_length_3755_cov_2.951892, whole genome shotgun sequence.
ACCESSION   DAOOQX010000123 DAOOQX010000000
VERSION     DAOOQX010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29158_length_3755_cov_2.951892"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1723)
                     /locus_tag="PLX53_02895"
     CDS             complement(<1..1723)
                     /locus_tag="PLX53_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HOY72056.1"
                     /translation="MEITRTFDILEAGKETYENRPVLASRQNGTWEEYSIDKYSQIAH
                     NLSYGLMALGLRPGDRVATVSGNRPEWNFVDMALSQAGMVHVPIYPTISLDEYRYILK
                     HSGVKMIFAGDKSIYGKLQPLTGSLENFIGMYCFQNNNELPSWKEIAEKGKSVANSYI
                     DELFRIKKSISADDVVSLIYTSGTTGISKGVMLTHNNFLSNVRACTGLFPLEPGDKAV
                     SFLPLSHVFERMVNYYYQSIGLTVYYIDNLANLGNVISEVKPQIFMTVPRMLEQTYNK
                     IIAVGKDLSGLKKQVFFWAVNLGYHYNENGNSQYYNMKLRLARKLVFEKWQEAMGGKI
                     KLIVSGGAPLQPRLMRIFWAAGMPVYEGYGLTETSPVIAVNHSATPGNVRFGSVGPVL
                     SNVEVKISNDGEILMRGPSLMKGYYNAPELTSQAIDSDGWFHTGDIGHLDEGKFLTIT
                     DRKKEIFKLSNGKYVAPQLIENKLKESFFIEQCMVVGENEKFVSVLISPNFSFLHDWC
                     SHHKIHFQNNQELITLPEVISRFQKEINGINKQLSDYEQIKRFRLVPEQWSQETGELS
                     PTLKLRRK"
     gene            1818..2693
                     /locus_tag="PLX53_02900"
     CDS             1818..2693
                     /locus_tag="PLX53_02900"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HOY72057.1"
                     /translation="MRKVIFCIIVCLTAGFSFAQDKNAYTIFDSKGNVASYSDLVNRS
                     AATDVVFFGELHDNPIAHWLELELAKDLFRAKNGSLIMGAEMFEADNQLILDEYMSGL
                     ISMKRFEDEAKLWNNYQTDYKPLVEFARESGVRFVATNIPRRYASAVAGGGFEVLNSF
                     PDEARKFFAPLPVAFDPELPGYKAMMGMDNMHGAGMQASQNLPKSQALKDATMAHFIM
                     ANFKEGSTFIHYNGSYHSDNKEGIVWYINRTNPNLRVVTVATVMQTDVDKLDAANLGI
                     ADFIIMVPESMTRTY"
     gene            complement(2747..>3755)
                     /locus_tag="PLX53_02905"
     CDS             complement(2747..>3755)
                     /locus_tag="PLX53_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HOY72058.1"
                     /translation="NVSKTQPVVVSEFIEGAKEIEFDAVANNGEVMFDAISEHIEFAG
                     VHSGDATLVFPPQKLYFETIRRIRKIARAIAKELMITGPFNMQLLALNNEVKVIECNL
                     RASRSFPFVSKVLGFNFVEAATEYMLGRTPGYLPPSMFELDHVGIKASQFSFSRLSKA
                     DPVLGVEMASTGEVGCLGTDFDDALLKSMLSVGYRVPQKSILVSSGPLRSKLALVEPL
                     RLIERCGITIYATRGTAKFLAENDVNTIPVAWPDEDSQPSSIELIGSKQVELVINIPK
                     NLSREELNNDYDIRRATVDYNIPLITNARLAAAFLMAICNKNINTLDLRSWDEFFETK
                     Q"
BASE COUNT          939 a          873 c          879 g         1064 t
ORIGIN      
        1 acttccgacg gagtttgagc gttggtgaaa gttcgcctgt ttcttgcgac cattgctccg
       61 gaaccagcct aaagcgtttg atctgctcgt agtcgctcag ctgcttgttt atgccattta
      121 tttctttttg aaatcgggaa atcacctcgg gtagggtgat aagttcctgg ttgttctgga
      181 agtgtatctt gtggtggctg caccaatcgt ggagaaagct gaagttgggt gaaatcagaa
      241 cgctgacaaa cttctcgttt tcaccaacta ccatgcactg ctcaatgaaa aatgattctt
      301 tcagcttgtt ttcaatcagc tgaggggcaa catacttgcc gttggaaagc ttaaagattt
      361 cttttttcct gtcggtaatg gttaggaatt ttccctcatc aaggtgtcca atgtcaccgg
      421 tatgaaacca tccatcggag tcaattgctt ggcttgtaag ctcaggagcg ttatagtagc
      481 ccttcatcaa gctggggccg cgcatgagta tttctccatc gttggatatt ttcacctcaa
      541 cattagaaag gacagggcca accgacccaa atcgaacatt cccaggggta gcgctgtggt
      601 tgacggcaat cactggtgag gtttccgtta gcccgtagcc ctcgtaaacc ggcatgccgg
      661 ctgcccagaa aattctcatc aaacggggtt ggagcggtgc accgccgctt acaatcagct
      721 ttatctttcc tcccatcgct tcctgccatt tctcaaaaac cagcttacgg gccagtctca
      781 acttcatgtt gtagtactgg ctattgccat tctcgttgta gtggtacccc agattaacag
      841 cccagaagaa aacctgtttc ttcagaccgg aaagatcctt tcctaccgct ataattttat
      901 tgtatgtttg ctcaagcatt cgcggtacgg tcatgaatat ctgtggcttt acctcgctga
      961 tgacattccc caggttggcc aggttgtcga tgtagtaaac tgtaaggcca atgctttggt
     1021 agtagtagtt caccatacgc tcaaatacgt ggctaagtgg aagaaagcta actgccttat
     1081 ctcctggttc taacggaaaa agtccggtgc aagcccttac attgctgaga aaattattat
     1141 gggtaagcat tacacctttt gatatgcccg ttgttcccga agtgtagatt agtgaaacaa
     1201 catcatcggc actaattgat ttttttattc tgaaaagctc atcaatatag ctgttggcaa
     1261 cacttttccc tttttcagca atttccttcc agctgggaag ttcgttatta ttctgaaagc
     1321 agtacatgcc gataaaattc tcaaggctac cggtgagggg ttgaagtttg ccgtaaatac
     1381 tcttatcgcc tgcaaaaatc atttttaccc ccgaatgctt aaggatgtac ctatactcat
     1441 caaggctgat ggtggggtag atgggtacat gcaccatgcc tgcctgcgaa agggccatgt
     1501 caacgaagtt ccattcgggt cggttacccg acacggtggc caccctatcg ccaggtctta
     1561 gacctagggc catcaatccg tagctcaggt tgtgcgcaat ttgtgagtac ttatctatgg
     1621 agtactcctc ccaggttccg ttttgcctgg atgccagaac tggacgattc tcataggttt
     1681 cctttccggc ttccagaatg tcgaatgttc gtgtgatttc catatcgcca tgcttagaaa
     1741 aaattgttaa aaatgtttac tttagctcgt tgtttagcta aaggtgtaaa tcccaggttt
     1801 ggcaaatata aaaaaatatg agaaaagtca ttttttgcat aatcgtttgt ttaacagcag
     1861 ggttttcctt tgcccaggat aaaaatgcct acaccatttt cgattcaaag ggaaatgtgg
     1921 cttcgtactc tgatttagtt aatagatcgg cagctaccga tgttgtgttt tttggagagc
     1981 tccacgataa ccccatagcc cactggctgg agctggagct ggcaaaagac ctttttaggg
     2041 cgaaaaacgg cagcctgatt atgggagccg agatgtttga ggccgataac cagctcatac
     2101 tcgatgagta catgtctggc cttatctcca tgaaacgctt tgaggatgag gcaaaacttt
     2161 ggaacaacta ccagaccgac tataaaccgt tggttgagtt tgccagggaa agtggggtta
     2221 ggttcgttgc caccaatatt ccccgcaggt acgcctctgc agtggctgga ggaggctttg
     2281 aggtgctgaa tagttttcct gatgaagccc gaaaattttt tgcaccctta ccggttgcct
     2341 tcgatcctga acttccgggc tataaggcta tgatgggtat ggacaacatg cacggagctg
     2401 gcatgcaggc ctctcagaac ttgcccaaat cgcaggcatt aaaggatgcc accatggctc
     2461 atttcataat ggccaacttt aaagaaggtt ccacctttat ccactacaat ggttcctatc
     2521 attccgataa caaggaaggt atagtctggt atatcaaccg gaccaacccc aacctaaggg
     2581 tggtaaccgt ggctactgtg atgcagaccg atgttgacaa gttggatgct gcaaatttgg
     2641 gcattgccga tttcatcata atggttcccg aaagcatgac ccgaacctac tagtcggtgc
     2701 ttaaacgcag gaaaatataa ttaacgttca gaaatacatg catttattat tgtttggttt
     2761 caaaaaattc atcccagctt cttaggtcga gagtgtttat gtttttattg cagatggcca
     2821 ttaggaatgc tgctgccaac cgggcgttgg taataagcgg aatgttgtag tcaaccgttg
     2881 cgcgccttat gtcgtaatcg ttgttcagct cctctcgtga taggttttta ggtatgttga
     2941 tcaccagctc aacctgttta ctaccaatta gctcgatact gctaggctgg ctgtcctcat
     3001 cgggccatgc caccggaata gtgtttacgt cattttcggc aaggaatttg gcagtgccac
     3061 gagtggcata gatagtaatg ccgcaacgct caattagtcg taaaggttct actaaggcca
     3121 gcttcgaccg taatggtccc gacgaaacga gaatggattt ttgcggtact cggtagccca
     3181 ccgaaagcat cgatttcaga agggcatcgt caaaatcggt tcccaaacag ccaacctctc
     3241 cggttgaggc catctcaacg cccagtaccg gatcggcctt tgaaagacgg ctaaatgaga
     3301 actgcgacgc ttttatgccc acatggtcaa gttcaaacat ggaaggaggt aggtagccgg
     3361 gtgtccgacc cagcatgtac tccgttgcag cctcaacaaa gttgaagccc aaaacttttg
     3421 agacaaatgg gaagctcctg cttgctctca ggttgcactc aatcaccttg acctcgttgt
     3481 tcagtgcaag cagctgcatg ttgaatgggc ctgtaatcat gagctcctta gctattgccc
     3541 tggcaatttt tcggattcga cggatggttt caaagtatag cttctgcggt ggaaaaacca
     3601 gggttgcgtc accggagtgc acacctgcaa attcaatatg ctccgaaatg gcgtcgaaca
     3661 tcacctctcc gttgttggcc acggcatcga attctatttc cttggcaccc tcgatgaatt
     3721 cgcttacgac aaccggctgg gttttggata catta
//
LOCUS       DAOOQX010000124         3748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_29227_length_3748_cov_3.480097, whole genome shotgun sequence.
ACCESSION   DAOOQX010000124 DAOOQX010000000
VERSION     DAOOQX010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29227_length_3748_cov_3.480097"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLX53_02910"
     CDS             complement(<1..218)
                     /locus_tag="PLX53_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY72059.1"
                     /translation="MKVVLITGVSSGFGRAIVLRLANKEFKVYGTVRRKVDPIPGVSY
                     LNMDVTSRESVEKAVGEVYGKEGRIDVL"
     gene            complement(230..1438)
                     /locus_tag="PLX53_02915"
     CDS             complement(230..1438)
                     /locus_tag="PLX53_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOY72060.1"
                     /translation="MGKLQERYSQYTLPQQIKAEGIYPYFRAIESEQSTVVTMKGKQV
                     LMFGSNSYLGLANHPRIKEAAKKAIDKYGTSCSGSRFLNGTLDIHLELEEKLAKLVGK
                     ESAICYSTGFQVNLGVVSALCSRGDYLLLDCLDHASIIEGSRLSFSKILKFDHNDMSS
                     LEAKLKLCEPDRIKLIVVDGIFSMEGDIANLPVIVQLAEKYDASIMVDDAHSLGVIGD
                     GGRGTASHFNLTPKVDLIMGTFSKSFASLGGFIAGDESVINYLRHNSRSLIFSASMTP
                     ASVASVSTAIDIMLEEPERMKHLWDLTRYAHEAFKSHGIYIGKSETPIIPLYVRDDIK
                     TLQLTKMLLDEGIFINPVITPAVPKEDSMIRFSLMATHTFEQLDIAIDKIVGSFKKLG
                     LLTTSTVKNP"
     gene            complement(1450..1911)
                     /locus_tag="PLX53_02920"
     CDS             complement(1450..1911)
                     /locus_tag="PLX53_02920"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOY72061.1"
                     /translation="MPKYFKFIASRLIGTIVDTLVLWLLTSFIFSSYVGAYIIAPTIS
                     FEFAVFSNFLFSYFWIWSKRVAVKNNRSFFSHFLSFNISSFLGFIVKMGFLLLFSKLL
                     KWSVVYCNFLALLISGVVNFGLSELVVFKKSPVVDSVDEKILVRRNKSGKF"
     gene            complement(1918..2622)
                     /locus_tag="PLX53_02925"
     CDS             complement(1918..2622)
                     /locus_tag="PLX53_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOY72062.1"
                     /translation="MNGKTEVRIQTSILNKMEKRILVAMANRMPSWITSDNLTVVGVI
                     GALTTGVGYILSSWNLNFLWLASFGLILNWFGDSLDGTLARVRNAQRPIYGFYIDHNI
                     DGLTITIMCIGAGLSPMIKFSTAMLILVGYLLLSIFTYINAYLKGEFRISYGNLGPTE
                     FRLIVIIINTMFIYFPFKDMYLHIMKYEVGVYDAIGFGIAILLFFIFLISFFVFLRKY
                     KQVDPPRWVDNHNNSN"
     gene            complement(2971..>3748)
                     /locus_tag="PLX53_02930"
     CDS             complement(2971..>3748)
                     /locus_tag="PLX53_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOY72063.1"
                     /translation="TARMFDLKVSSYVDERMDPDKSTEAACRYLEYLYRTFNSWHLAI
                     AAYNVGPEPIRKAIARNHGETNFWKLYDDLPESAQNYVPAFIAAAYVMQHYGDHGIFP
                     ARPTIAFAQTDTVHVAFAVNFSTLSSALNIPVELLRYLNPMYRIDYIPKDSQRQVLRL
                     PSDKAVLFTANKESILFNSPKTTHQQSITSGSASGSSKKSKMLIYTVKDGDSINKIAI
                     NYYCTLDLIRQWNPGLDTLLNVGQTLTLWVSEDELQKINS"
BASE COUNT         1109 a          807 c          811 g         1021 t
ORIGIN      
        1 atcagcacgt ctatacgccc ctcctttcca taaacctccc caacagcctt ttcaaccgat
       61 tcccggctcg ttacgtccat gttgaggtag ctaacacctg ggattggatc gaccttccgg
      121 cgcacggtgc cataaacctt aaattcctta ttggcaagcc gcagtacaat agcgcgacca
      181 aatccagagg aaacacccgt tatgagaaca accttcattt gcttgtaggc tagggatttt
      241 tgacggttga ggtggtaagc aacccgagct tcttaaatga acccacaatt ttatctatgg
      301 caatgtccag ctgttcgaag gtatgggtag ccatgagtga gaagcgtatc atggagtctt
      361 ccttcggaac agcaggtgta ataacagggt tgatgaatat tccctcatct aaaagcattt
      421 tagtaagctg aagggtttta atatcatcgc gaacgtaaag aggaataatg ggggtttcgc
      481 ttttgccaat gtaaatgcca tgactcttaa aagcctcatg ggcatatcgg gttaaatccc
      541 aaagatgctt cattcgttcc ggttcttcga gcattatatc aatggcagtg ctaacgctgg
      601 ccaccgaggc aggagtcatg ctggcgctaa aaatcagcga gcgggagtta tgcctaaggt
      661 agttgataac cgactcgtct ccggctataa aacctcctag tgaggcaaag gattttgaaa
      721 aagttcccat aatcaggtcc accttagggg taaggttgaa gtgcgaggcg gttcccctgc
      781 ccccatcccc aattacaccc agcgaatggg catcgtcaac cattatgctt gcatcgtact
      841 tctcggctag ctgaacaatg acgggcaagt tggctatatc gccctccatg gagaatattc
      901 cgtcaaccac aataagctta atccggtcgg gctcacaaag cttaagcttg gcttccagcg
      961 agctcatatc attatggtcg aacttcagaa ttttggaaaa tgacagccgg cttccttcta
     1021 taatagaggc atggtcgagg caatcgagca ggaggtagtc accacggcta caaagagccg
     1081 aaacaactcc cagattaacc tggaagcctg tgctgtaaca gattgccgat tccttaccaa
     1141 ccagcttagc cagcttttct tcgagctcaa ggtgaatgtc gagcgttcca ttcaggaatc
     1201 ttgaacccga acagctggtg ccgtacttgt ctatcgcctt ctttgccgcc tccttaatcc
     1261 ggggatggtt ggccaaaccc agatagctgt tagagccaaa cattaaaacc tgcttgccct
     1321 tcatggtaac cacggtgctt tgctcggatt caatcgccct aaagtaaggg taaattccct
     1381 cagcctttat ttgctggggc agggtgtact gcgagtaacg ttcttgaagt tttcccatat
     1441 ccattcaagt taaaacttac cagatttatt tctacgaacc aatatctttt cgtccaccga
     1501 gtccactacg ggggactttt taaatacaac cagctccgaa agtccgaagt tgacaactcc
     1561 ggaaatgagc agtgctaaaa aattacagta aaccacactc cacttcaaca gcttgctaaa
     1621 tagtagcaaa aatcccattt taacaataaa tcccagaaaa gaagatatgt tgaatgataa
     1681 aaagtgacta aaaaaagatc tgttgttttt tacagctacg cgcttactcc atatccaaaa
     1741 gtatgaaaat aaaaaatttg aaaaaaccgc aaattcgaat gagattgtag gtgctatgat
     1801 gtatgctcca acataggagg aaaagataaa gctcgtgagc agccacagca ctagcgtatc
     1861 gacaattgtc ccaattagcc ggctggcaat aaatttgaag tacttgggca tcatgggcta
     1921 attcgaattg ttatggttat cgacccaacg gggagggtca acctgtttgt acttccttaa
     1981 aaagacaaag aagctgataa gaaaaataaa gaaaagcaga atggcaattc caaacccaat
     2041 ggcatcgtaa actccaacct catacttcat aatgtgaagg tacatatctt taaatggaaa
     2101 gtatataaac attgtgttga taatgattac gataagcctg aattcggtgg gtcccaggtt
     2161 gccgtatgat attctaaact caccttttag gtaggcatta atataggtga aaattgacag
     2221 gagcaggtaa ccaaccagta tcagcatggc ggtggaaaac tttatcatgg gcgaaaggcc
     2281 ggcgccaata cacataatgg taatggtgag cccatcaatg ttatgatcta tataaaatcc
     2341 gtaaattggc ctttgagcat ttcttaccct tgccaatgtt ccatcaaggc tatcgccaaa
     2401 ccaatttaga ataagcccaa acgaggcaag ccataaaaaa ttaaggttcc agctggaaag
     2461 tatgtatcct accccggtcg ttaatgcgcc gatcacaccc actacggtga ggttatcaga
     2521 ggttatccag ctgggcattc gattggccat cgccaccaaa atcctttttt ccatcttatt
     2581 cagaatggaa gtctgtattc ttacttcggt tttaccattc attttaacat atttttatta
     2641 caatttccac tgcatggttt tgaaccaggg tgctatatta ataaattgtt aagaaaaatc
     2701 atattgtcct ataaccataa ttgcattact cgtaagccga aataaatagc aatattctaa
     2761 ttaccaataa tttaatactg atactgacta tttgtattaa atagtcaaaa ataaaaaata
     2821 tttatacccg ttaagatttt gttataaaat tcttaacaag acaaaaatct ctgtggctga
     2881 aacgcaagaa actaatactt tgctagccca tagaggagac ttggtgcata tacagctgaa
     2941 agtgatcaac aagcgcagcc gaagttaggg ctagctgttg attttttgga gttcgtcctc
     3001 gctaacccaa agcgtgaggg tttggccaac gtttaggagt gtgtcaaggc caggattcca
     3061 ttgcctaatc aggtcaagcg tgcagtagta gttgatggca atcttgttta tactatcccc
     3121 atccttaacc gtatagatta gcatttttga tttttttgaa cttccacttg ctgacccgga
     3181 agttatggac tgctgatggg ttgttttggg tgagttgaaa agtatggatt ccttattagc
     3241 tgtaaataaa acagctttat cggatggcag ccgtaagacc tgccgttggc tgtctttggg
     3301 gatatagtcg atccggtaca tggggttcag gtaccggaga agttccactg ggatgttaag
     3361 cgccgaagat agtgtggaga agttgacggc aaaggctaca tgaacggtat cagtttgggc
     3421 aaaggcaatt gtagggcgag caggaaaaat gccgtggtcg ccataatgct gcattacgta
     3481 tgcggcagca ataaaggcag gaacatagtt ttgagctgat tcaggcagat catcgtaaag
     3541 cttccaaaag ttggtttcac catgatttcg tgcaatagct tttcttatag gttctggtcc
     3601 aacattatag gctgcaatgg ctaaatgcca gctattaaat gtcctgtaaa ggtactccag
     3661 gtaacggcat gctgcttcgg ttgatttatc cggatccatc cgctcatcaa cgtaggatga
     3721 aacctttaaa tcgaacattc gcgctgta
//
LOCUS       DAOOQX010000125         3730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_29425_length_3730_cov_3.284082, whole genome shotgun sequence.
ACCESSION   DAOOQX010000125 DAOOQX010000000
VERSION     DAOOQX010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29425_length_3730_cov_3.284082"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLX53_02935"
     CDS             complement(<1..662)
                     /locus_tag="PLX53_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOY72064.1"
                     /translation="MIYYLVQYIQKQFDFPGAGLFRYISFRSALAFILSLLFALFIGK
                     RIIRYLQRKQIGEEIRNLGLEGQLQKKGTPTMGGIIVIAATLISVLLFADLLNIYIII
                     MLVTTVWMGAVGFTDDYIKVFLKNKDGLSGKFKILGQVLLGLFVGLTMWLSPEIMIRD
                     HVRPNVQSEVVVTSEYSGSEPVSVSKGKKTTQTTIPFFKNNEFDYAYLNPFKGKWRDA
                     IT"
     gene            complement(659..2125)
                     /locus_tag="PLX53_02940"
     CDS             complement(659..2125)
                     /locus_tag="PLX53_02940"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOY72065.1"
                     /translation="MAVGLTKILPKALVNEVVGNPDIEVEQLTFNSRVAGANSCFFAI
                     RGTQADGHRFIPQAVENGASAIVCEAMPVSFAPNVTYVRVSDSSEALGLMASAFYGNP
                     SSNLKLVGVTGTNGKTTTATLLYRIVGMLGFKAGLLSTVVNQVVDEKIPTTHTTPDPI
                     ELNRLLRRMVDAGCSYCFMEVSSHAVVQKRIAGLTFAGGIFTNITHDHLDYHKTFDEY
                     IRAKKMFFDQLPSESFALVNADDRNGMVMVQNTKAKVSTYALHSIADFRCRVVESHFD
                     GMLLNMDGLEVWTRLIGEFNAYNLLAIYASLLLLGFDKTDVLLQLSMVNSVSGRFEYV
                     SSSGGITAVVDYAHTPDALVNVIETIKKIKSSGQRLITVVGAGGNRDKTKRPVMARVA
                     VQNSDLVILTSDNPRFEEPEGIINDMKAGVDEEFTKKLVTIVDRHEAIRTACLLANKE
                     DIILVAGKGHENYQEVKGVKHHFDDKEVLAEIFNELHI"
     gene            complement(2138..>3730)
                     /locus_tag="PLX53_02945"
     CDS             complement(2138..>3730)
                     /locus_tag="PLX53_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOY72066.1"
                     /translation="ELAQVLAHCSGGVAVGIEGAFDYALKGKAGLKVHERIPGNSWVE
                     VKSMNQIDPEDGCDVVTTIDVALQDVAEAALRKHLGNHDADHGCAVLMEVSTGDIKAI
                     ANLKRNEDGTYSEVYNYAIGESTEPGSTIKIATLINLLEDGFVELNDTVNTGNGKLRM
                     YDRIITDSKEGGHGKISVKEVFEVSSNVGVAKLVTKYYRGKESHYVDRLYSMKLNQPL
                     GIGIPGEASPQIKYPTDRYWSGMSLPMMSIGYELKLTPLQILTFYNAIANDGRMVKPR
                     LVKSLERHGMVVQTFSPEVINPSICSRSTLRKVNEVLEGVVESGTAQNLKSTPYKIAG
                     KTGTAQLAKGRRGYKIGGSVSYQASFVGYFPSDKPRYSCIVVVNAPSQNIYYGNLVAG
                     PIFKEISDKVYATTPDWFREIDNGGSLKELPESKSTRLVMLKEILKQLDVPYQIANSK
                     AEWVSTKRDSNEIALNTLSFSSKAVPSVVGMGLRDAIFLLENTGLHVSFNGRGTVRSQ
                     SIAPGTAIKRGSNINLELSMSN"
BASE COUNT          939 a          951 c          840 g         1000 t
ORIGIN      
        1 caggtgatgg catcgcgcca cttccccttg aaagggttta ggtacgcgta gtcaaactcg
       61 ttgttcttaa agaagggtat ggtggtttgg gttgtttttt tgcccttact aacgctaacg
      121 ggttccgaac cggagtactc cgaggtaacc accacttcgc tttgaacatt tggtcttaca
      181 tggtcgcgaa tcataatttc ggggctgagc cacatagtga gaccaacaaa tagccctagt
      241 agaacttgtc ctaaaatttt gaacttaccc gaaaggccat ccttgttttt caggaatact
      301 ttgatatagt cgtcggtaaa gccaactgca cccatccaaa cggtggtcac cagcattatg
      361 atgatgtaaa tgttaaggag atcggcaaaa agcagaaccg agatcagggt ggcagcaatg
      421 acgatgattc cccccatggt gggtgttccc tttttctgca gctgcccctc aagcccaagg
      481 ttgcgtatct cctcaccaat ctgctttcgt tgcaggtacc taatgattcg cttgccaatg
      541 aaaagggcaa aaagaaggga caggataaaa gccagggccg agcggaacga aatgtagcgg
      601 aaaagaccgg ctccggggaa atcgaattgc ttttgaatgt actgtaccag gtagtaaatc
      661 atatatgcag ctcgttaaat atttcagcta aaacttcctt atcgtcaaag tggtgcttta
      721 cccctttaac ctcctgatag ttttcatggc ctttgcctgc aaccagaatg atgtcttcct
      781 tgttggccag caggcatgcc gtcctgatgg cttcatgtct atctacaatg gtaactagct
      841 tcttggtaaa ctcctcatca acaccggcct tcatatcatt aataataccc tcgggctcct
      901 caaaacgggg attatcggaa gtaagaatga ccaagtcgct gttctgaaca gccactcttg
      961 ccatgacagg tctcttagtc ttatcgcggt ttccaccagc cccgaccacc gttataagcc
     1021 gttgtccgct actcttaatc ttttttatag tttcaattac gttaaccaga gcatcggggg
     1081 tgtgggcata gtccaccact gcggtgatac cgccacttga gctaacatac tcaaagcggc
     1141 cgctaaccga attaaccatg cttagctgga gcaatacatc ggttttgtca aacccaagta
     1201 gcaaaagcga ggcataaata gccagcaggt tataggcgtt aaactcaccg ataagccggg
     1261 tccaaacctc cagcccatcc atattcagca gcattccatc gaagtggctt tcaaccaccc
     1321 tgcagcggaa atcggcaatg gagtgaaggg cgtaagtaga gactttggct ttggtgttct
     1381 gaaccatgac cattccattc cgatcgtcgg cattaaccag ggcaaatgat tcggaaggca
     1441 gctggtcgaa gaacattttt ttagccctga tgtactcgtc aaatgtttta tggtagtcga
     1501 gatgatcgtg ggtaatgttg gtgaatattc cgccggcaaa agtaaggccg gcaatccttt
     1561 tctgcacaac ggcatgtgag ctcacctcca tgaagcagta gctacacccc gcatcaacca
     1621 tacggcgtag taacctgttc aattcaattg ggtcgggagt tgtatgcgtt gtgggtattt
     1681 tctcgtctac cacttggttc accactgttg agagcaagcc tgccttaaat cccagcatcc
     1741 caacaattcg gtaaagtaga gttgccgtgg tggttttacc attggttccg gtaacaccta
     1801 ccagcttaag gttcgacgac gggttaccgt agaatgcaga agccattagc cccagggctt
     1861 ccgaggaatc cgaaacccta acgtatgtta catttggggc aaagctaacg ggcattgctt
     1921 cgcacacaat agctgaagcc ccgttttcga ctgcttgagg gatgaaccgg tgcccatcag
     1981 cctgagttcc ccttatggca aaaaagcaag agttggcccc ggcaaccctc gagttgaagg
     2041 ttagctgttc cacctcgata tcggggtttc caactacttc gttgacaagc gcttttggta
     2101 atattttagt taagcctact gccatgatat ataaattcta attgctcata ctaagttcaa
     2161 ggttgatgtt gcttccccgc ttaatggctg ttcccggggc aatggattgc gaacgaacag
     2221 tacccctccc gttaaagcta acatgcaacc cggtgttttc gagaagaaaa attgcatccc
     2281 taagccccat cccaacaacc gacggaacag cttttgatga aaaactaagc gtgttaagcg
     2341 caatctcgtt gctatcgcgt ttggttgaaa cccattcagc tttactgtta gcaatttgat
     2401 aaggcacatc gagctgcttc aatatttcct ttagcattac caatcgggtt gatttagatt
     2461 cggggagctc ctttaggctt ccaccgttat caatctccct gaaccaatcg ggcgtggtag
     2521 catacacctt gtcggaaatt tctttaaata ttggcccggc aaccaggttt ccatagtaga
     2581 tattctgcga aggtgcgtta accaccacta tgcaggaata tctaggcttg tccgaaggaa
     2641 aataacccac aaacgatgcc tgatagctaa cgctaccgcc aattttgtaa ccccttcgcc
     2701 ccttggcaag ctgtgctgtt cccgttttgc ctgcaatttt atagggagta cttttaagat
     2761 tctgggcagt gccgctctca actacccctt ccagcacctc gttcaccttt cggagggttg
     2821 acctgctgca aatggagggg ttgataactt cgggcgagaa ggtttgtaca accattccgt
     2881 gtcgctctag cgacttaacc agccttggct taaccattct cccgtcgttg gcaatagcat
     2941 tgtaaaaggt aagaatttga agcggtgtaa gcttgagctc gtaccctatg ctcatcattg
     3001 gtaacgacat ccccgaccag tacctgtcgg tggggtactt gatctgtggg ctagcctcgc
     3061 ccggaatgcc aattcctaag ggttggttga gcttcatgga gtagagcctg tcaacataat
     3121 gactttcctt tcccctatag tacttggtaa ccagcttggc cactccaacg tttgatgaaa
     3181 cttcaaacac ctccttaacc gaaattttac catgcccccc ctccttgctg tcggtaatta
     3241 tgcggtcgta catccgcaac ttgccgttgc cggtgttaac cgtatcgttc aattcaacaa
     3301 agccatcttc cagcaggtta atgagggtag ctattttaat ggtggagccg ggctcagtac
     3361 tttcgccaat ggcatagttg taaacctcag agtaggtacc atcctcgttg cgcttaagat
     3421 ttgcaattgc cttaatatcg ccggttgata cctccatgag aacagcgcaa ccatggtcgg
     3481 catcgtgatt tccaaggtgt ttacgaagtg ccgcttcagc aacatcctgt agggcaacat
     3541 caatggtggt aaccacatca caaccatcct ccgggtctat ctggttcata cttttcacct
     3601 caacccaact attcccagga atacgttcat gcaccttaag ccctgcctta cccttaagtg
     3661 catagtcgaa tgctccctca atgcctacgg caacaccgcc gctgcaatgg gcaaggactt
     3721 gagccagctc
//
LOCUS       DAOOQX010000126         3716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_29590_length_3716_cov_2.898662, whole genome shotgun sequence.
ACCESSION   DAOOQX010000126 DAOOQX010000000
VERSION     DAOOQX010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29590_length_3716_cov_2.898662"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1070
                     /gene="murB"
                     /locus_tag="PLX53_02950"
     CDS             57..1070
                     /gene="murB"
                     /locus_tag="PLX53_02950"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOY72067.1"
                     /translation="MPVIKKNVSLKPYNTFGVDVSASYFVKANTLEKILYAINFASYN
                     QLPIFIIGGGSNMLLTKNLNGVMVNPFIQGLTLTEETDSNAIVKVGAGMTWDALVNIA
                     VDHGWGGIENLSNIPGSVGAAPIQNIGAYGVEAKDAIFKVEAIEIKTRKSLEFSNSEC
                     RFGYRDSIFKQELRGKVIVTHVWFKLSKKPEYQLAYGNLKEEVEKLGGVSLKNIRQAV
                     INIRSAKLPDPAVIGNAGSFFKNPVVEDVPFQKIQQDYPDVPHFSVSESHVKIPAGWL
                     IEQCGWKGKRVGSCGVHDKQALVLVNYGGATGEEILTLARSIQQSVYDRFHINLDMEV
                     NIC"
     gene            complement(1109..2890)
                     /locus_tag="PLX53_02955"
     CDS             complement(1109..2890)
                     /locus_tag="PLX53_02955"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOY72068.1"
                     /translation="MDTKINERLQLLRRQMKSRGFDAYIIHHSDPHISEYIPDYWKVR
                     EWASGFSGSAGTLVLTEKKAALWTDSRYFLQAEQQLAGTGIELCKQGLPDTPDIPGWI
                     KLNLEAGSTVGCNGGIISVSAARYLSKSLEQLGFKCSFDDDIASTVWLDRPPLPTAKA
                     YIHAAEFAGISAGDKLESIRRNLLNINATACLMCSPDEVCWTLNLRGSDIQFNPVVLC
                     YAYVDLERAILFVDGRKLDDEISNFLTSAGVSTMGYEQVGNFISNISRHTVLVVDSDK
                     TNYKLYNQIPQTVKIVEKIGIVTEIKAKKQKPEVEGIHQAMVQDGVALVNFFYWLEEV
                     LGRERLTELTVAKKLLDFRSKQQHFVSESFETIAGYRDHGAIVHYSATPESDYTIEQD
                     GFLLIDSGGQYLNGTTDITRTVHLGKPNPDEMKHYTLVLKGMISLAKARFPFGTRGSQ
                     LDTLAKMALWSNHLTYGHGTGHGVGYFLNVHEGPQQIRPENHLPIEPGMVMSDEPGIY
                     ISGCYGIRIENLILCTDDISNDFGRFLTFETLTLCPIDTKPIAVSMLSDDERDWLNHY
                     HATVYEKLSPYLDEEHKTWLKKKTLPL"
     gene            complement(2934..>3716)
                     /locus_tag="PLX53_02960"
     CDS             complement(2934..>3716)
                     /locus_tag="PLX53_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOY72069.1"
                     /translation="NAENIFLKTDEGRLEQALANLLDGIFKTVERGTIELSCRKENSK
                     VMIFLRISGAGAESKVNESLLDQFRQTENIESPMQSLLGIRMARQIVTLLGGEMRFDI
                     GSNDNAEVEVLLPLTDSSTSGSNNNIEDNAPVYPDFKNKVILIVEDEEVNYLLFRELL
                     VSCGATTLWAKNGREAVNLVSTINQTIHLVVMDIQLPVMDGYAATMEIKQINPKIPVI
                     AETAYISADDRKKAEAAGCDGYITKPIEPKVFYEVLEIFLGT"
BASE COUNT         1020 a          866 c          799 g         1031 t
ORIGIN      
        1 ttccatgtta ccaattattg attacttttg ttgttgatga tgaaaatacc acaagcatgc
       61 cggttattaa aaaaaatgtt tcgttaaaac cctataatac atttggtgtt gatgtttcag
      121 catcctactt tgtaaaggcc aacacgctgg agaaaatcct ttacgcaata aattttgcat
      181 cgtacaacca gcttccaatc tttatcatcg gagggggtag caacatgctg ctcaccaaaa
      241 acctaaacgg cgtaatggtt aatcctttta ttcaaggttt aacccttacc gaggaaaccg
      301 actccaatgc aatcgtaaag gttggtgccg gaatgacctg ggatgcattg gtaaacatag
      361 ctgtcgatca cggatggggt ggaatcgaaa acctatcaaa tattcccgga agcgtaggtg
      421 ctgcacccat ccaaaatatt ggtgcctacg gggtagaggc caaggatgct attttcaagg
      481 tcgaggcaat tgagataaaa acacgtaaat cactggaatt ttcaaatagc gaatgccgtt
      541 ttggctaccg tgatagcata ttcaaacagg agctcagggg caaggttatt gttacccacg
      601 tttggtttaa actatcaaaa aaacctgaat accagctagc ttacgggaat cttaaggagg
      661 aggttgaaaa gcttggtggc gtttcgttaa aaaatatccg ccaggcagtt ataaacatta
      721 gaagcgccaa gctccccgat ccggctgtta tcggaaatgc cggcagcttt ttcaaaaatc
      781 ccgttgttga ggatgtgccg tttcaaaaaa ttcaacagga ctaccccgat gtaccccatt
      841 tcagcgtgtc ggaatcccat gtaaaaattc cagccggatg gcttattgag caatgcggtt
      901 ggaaggggaa acgggtaggc agctgcggcg tgcacgacaa gcaagcgctg gtgctggtta
      961 actatggggg tgcaacaggt gaggaaatat taaccctagc acgctcaatt cagcaatctg
     1021 tttacgacag gttccacatc aacctcgaca tggaggttaa tatttgctag ccacgttaga
     1081 atagacaaat aagctgatgt tgaatagctt acaaaggtaa agttttcttc ttcaaccaag
     1141 ttttgtgttc ctcatcaaga tagggcgaca atttttcgta aaccgttgca tggtagtgat
     1201 tcagccagtc ccgttcatcg tcgctaagca tacttaccgc aattggcttg gtatcaatgg
     1261 ggcaaagggt taacgtttcg aaagttaaaa aacggccgaa gtcgttggaa atatcgtcgg
     1321 tgcaaagtat aaggttttcg attcgaatgc catagcagcc agaaatatat attccaggtt
     1381 catcggacat caccatgccg ggctcaatgg gaagatggtt ttcgggacga atctgctggg
     1441 ggccctcgtg aacgttcaga aagtacccaa ctccatgccc tgttccatgc ccataggtaa
     1501 gatggtttga ccaaagggcc attttggcca gcgtatcgag ctgcgaacca cgtgttccga
     1561 aggggaacct ggccttggca aggctaatca tcccttttaa aaccagcgtg taatgcttca
     1621 tctcgtcggg attaggctta cccaggtgaa cagttcgggt aatatcagta gtcccgttca
     1681 ggtattgccc tcccgagtca ataagcagaa atccgtcctg ttctatggta tagtcagact
     1741 cgggagttgc gctatagtga actattgccc catgatccct ataaccagca atggtttcaa
     1801 aactttcgct tacaaaatgc tgttgtttgc tcctgaaatc aagcaacttt ttggctaccg
     1861 taagctctgt taacctctcc ctccccaaaa cctcctccaa ccagtagaaa aaattaacca
     1921 gggcaacccc atcctgcacc attgcctggt gaatgccttc aacctctggt ttctgctttt
     1981 ttgccttaat ttcagttaca attccaattt tctcgacaat ctttaccgtt tggggtatct
     2041 ggttgtacaa cttgtagtta gttttatcgg aatcaaccac caacaccgtg tgcctgctga
     2101 tgttagagat aaaatttcca acctgctcat agcccattgt agaaacgcct gcactggtaa
     2161 ggaaattaga aatttcatcg tcgagttttc tcccgtcaac aaaaagaata gcccgttcaa
     2221 ggtcaacata ggcatagcaa agcactaccg gattgaactg gatgtcgctt ccacggaggt
     2281 tgagagtcca gcaaacctca tcgggggaac acattagaca ggccgtagca ttaatgttaa
     2341 gcaagttacg acggatactt tctagcttat caccagcgga gattccggca aattcagctg
     2401 catgaatata ggctttagca gtaggaagtg gtggcctgtc taaccatact gtgcttgcaa
     2461 tatcgtcatc aaaactacat ttaaagccaa gctgttcaag gcttttcgaa agatatcgtg
     2521 cagcagaaac cgaaataatg ccaccattac acccaactgt tgacccagct tctagattca
     2581 actttatcca gcctggaata tccggtgtat cgggaagccc ctgcttacaa agttcaatgc
     2641 ctgtacctgc aagctgctgc tcagcttgaa ggaagtagcg cgagtcggtc caaagtgctg
     2701 cttttttttc ggttagcacc agagttccag ccgatccgga aaatccggaa gcccattccc
     2761 taaccttcca gtaatcggga atgtactcgc tgatatgggg gtcgctatga tgaataatat
     2821 aggcatcaaa acccctgctt ttcatctgcc ggcgcagcag ctgcaatctt tcatttattt
     2881 tagtatccat tcaatctttt tattaacgaa tcaacatcat gtgttatgta aaatcaggta
     2941 cccagaaaaa tttccaaaac ttcgtagaat actttaggtt caattggctt agtaatatac
     3001 ccatcgcatc cggcggcctc ggcctttttt cgatcgtcag ctgaaatgta tgcggtttca
     3061 gcaatgactg ggattttagg attgatctgc tttatctcca tggttgcagc atagccatcc
     3121 attaccggaa gttgaatatc cattacaacc aggtgtatgg tttgattgat agtgctaacc
     3181 aggttaacag cttccctgcc gttttttgcc cacagggttg ttgccccaca cgaaaccagc
     3241 aactccctaa acagcaggta gtttacctcc tcgtcctcaa caatcaggat aaccttgttt
     3301 ttaaaatcgg gataaacggg agcattgtcc tcgatgttgt tattactacc cgaagtactg
     3361 ctatccgtta ggggtaatag aacttcaacc tccgcattgt cgttggagcc gatatcgaac
     3421 cgcatctccc caccaagcaa tgtaacaatc tgcctagcca tcctaatgcc cagtagagat
     3481 tgcattggcg actctatgtt ttcggtctgt ctgaactgat cgagtaagct ttcgttaacc
     3541 tttgattcgg caccagctcc gcttattctt aaaaagatca tcaccttgct gttttctttt
     3601 ctgcagctca gctcaattgt gccccgttca acagttttaa agataccgtc caaaaggttg
     3661 gctaatgcct gttccaacct tccctcatcg gttttaagaa atatgttttc ggcatt
//
LOCUS       DAOOQX010000127         3703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_29711_length_3703_cov_3.182292, whole genome shotgun sequence.
ACCESSION   DAOOQX010000127 DAOOQX010000000
VERSION     DAOOQX010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29711_length_3703_cov_3.182292"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(327..1442)
                     /locus_tag="PLX53_02965"
     CDS             complement(327..1442)
                     /locus_tag="PLX53_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72070.1"
                     /translation="MVSKRIGHKPKIAAALALVQLLAIQQFCYGQDSTSTLGLNLYSS
                     AFFDNKEFTGDIKKGYTLPGFFIQPVINFSTDNYSVEAGFHLLYMAGTDSLEHLVPVL
                     TLARKLSKSSTILVGTLDARNGHFLPEPLFKSERVFTGQPETGVQFKHKTNNTFSDLW
                     INWEQFIKTGSPFQEQFTVGFTFQYLESNSKTPVFFTKTMALATHQGGQIDSTNLPVT
                     TIVNIGETAGILIPLKPGNANLSIEVSGFLSADKSPNPHLKYDQGYAFYPRIQMEWPF
                     TRFSFGYWHARSFINPRGEELFGSLSTVNPTLDSKIRKLVTCSLEYSKTLQQGFTFGV
                     GVNGYYDTRQGLLDYSYTFRMIFDGELVRFRRTGASR"
     gene            1698..2879
                     /locus_tag="PLX53_02970"
     CDS             1698..2879
                     /locus_tag="PLX53_02970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOY72071.1"
                     /translation="MKRKQKIQLIALAVLAFLAIFLSLPQNRYIVKALIYQQVNIDDY
                     KIFHNRTVRMGQPQPWKISPQYNHYQLSDKQLEYFERYKTVAYLVVKDTAILYESYWD
                     GYGSDSYSNSFSMAKSIVSLLVGCALQDGYIRSIDQPVADFLPEFRQDGRDKITIKHL
                     LTMSSGLSWDESYSTLFSLTTKGYYGNNLAGLVLGQHVVEEPGKRFMYRSGDTQLLSL
                     VVEKASGETLSDYASKRLWSKIGAEHDALWCLDRQNGVEKAFCCFNSNARDFARFGQL
                     VLNQGTWNGEQLIPKDYLAEALQPATYLIDAETGKPCDFYGYQWWRINMDGHEVNYAR
                     GLLGQYIFVIPSLNAVVVRLGHLRGDETIEGQPEDVYQYLRCGVEIIKQLQIPSEYER
                     K"
     gene            2866..>3703
                     /locus_tag="PLX53_02975"
     CDS             2866..>3703
                     /locus_tag="PLX53_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72072.1"
                     /translation="MSENDNLFLTLERLRVKLLAFPAADVTLFEKAKKKHISLFNPEQ
                     IEFVNDKPDILMFLTGGSEHIAIESVQEYRFYLMLASREANAWASATEVKAWMNMHNV
                     SSLLINTDDPLASTYIQEFYKASNGIRHLHGQRLGVVGKPSAWLVASTISPFLLQSRL
                     GIEQVDISWNEIMFDEIQQVSPDFTTLFGMADDKEELMASGRIFEGLASLIPFYRLNA
                     LSVECFPLVNQTSHTACLALAKLNLDGFPTACEADSCSAAGMMLANEVCGVIPWMANT
                     ILV"
BASE COUNT          977 a          797 c          896 g         1033 t
ORIGIN      
        1 aggataaggg ttaaaggata aaaaaaatgt ttgggtaacg atgaagcgtg tccgcctccc
       61 ggcggaactt gtccgcctcc cggcggattc atggaagcta ttccgcccct tcagggctta
      121 acgatgttgg aaacgtcctt atcaaggggc tttgcccctt gctgcttatt gcaccccgtt
      181 ggggctgaaa aatgaaaaag caaagaatat tttcaagttt tgacggactt aaaacaatga
      241 caggctttcg tggaatatta agttcaacgc taaatttcga ctgttaacaa ttaacaggtt
      301 cagttctaac gggtcttttc gagttgtcaa cgagatgccc cagttctcct gaaccgtact
      361 agctctccgt cgaaaatcat tctaaaggtg tagctgtagt ccagcaatcc ttgcctggta
      421 tcgtagtagc catttactcc aactccaaag gtaaaaccct gttggagggt cttactgtac
      481 tccagcgaac aggttacgag cttgcgaatc tttgaatcga gtgtagggtt aacagttgac
      541 agggaaccaa aaagttcttc acctctggga ttaataaatg atctggcatg ccagtaacca
      601 aatgaaaagc gggtaaaagg ccattccatt tgaatccttg ggtaaaacgc gtagccttga
      661 tcatatttaa ggtggggatt tggcgatttg tcagccgaaa gaaaacctga aacctcgatg
      721 ctaagattgg cattgcccgg tttgagagga attagtatac ctgccgtttc tccaatgtta
      781 acaattgtgg taaccggaag attggtgctg tctatctgac caccctggtg ggtagccaat
      841 gccattgttt tagtaaaaaa gacgggggtc ttactgtttg attccagata ctgaaaggta
      901 aatcccaccg taaactgctc ctgaaaaggg ctacccgttt ttatgaactg ctcccagtta
      961 atccacaagt cgctgaaggt attgttcgtt ttatgcttaa actgaacccc ggtttcgggt
     1021 tggccggtaa acacccgttc ggacttaaaa agtggttcag gaaggaaatg gccatttcgg
     1081 gcatccagtg tgccaaccag gatggtagag cttttggaaa gctttcgtgc aagtgtcagg
     1141 acagggacta aatgctccag gctatcggta ccggccatat atagaaggtg aaaacccgct
     1201 tcaacgctat agttatcggt actgaagtta ataacgggtt gtataaagaa tcccggcagt
     1261 gtgtagccct tttttatatc acccgtgaac tccttgttgt caaaaaaggc tgaggagtaa
     1321 aggtttaagc ctagtgtaga ggtgctatcc tgtccgtagc aaaactgttg aattgcaagt
     1381 agctgcacga gagctaacgc agctgcaatt tttggcttat gaccaattcg tttgcttacc
     1441 atgatgaata aaaagtttat ctaattgaaa atcgggtaaa ggtatgctct tttttgctag
     1501 tttttcctag ttttattgct aatttgatgt aatccgattt tgactatctt taaggtttac
     1561 aactgtttag atgatgatgc ttataggttg aggcagtttg gttcttctag ctgttagggt
     1621 ttatcatctt attataggta aactcctaag ggacggacga tttttttaac attgaccata
     1681 acactaaaag caaacccatg aagcgcaagc aaaaaattca acttattgcg ttggcggtgc
     1741 tggccttttt ggccattttt cttagcctac cccaaaatcg ctacattgtt aaagctttaa
     1801 tttaccagca ggtaaatatt gacgattata aaattttcca taaccggaca gtccgtatgg
     1861 ggcaaccgca accatggaaa atatctcctc agtacaacca ctatcagcta tcggataagc
     1921 agctggagta ctttgagagg tataaaactg ttgcctacct tgtggtgaaa gataccgcca
     1981 tactttatga gtcttactgg gatggttacg gctcagattc ctactccaac tcattttcta
     2041 tggccaagag cattgttagc ttgctggtag gttgcgccct tcaggatggc tacataagga
     2101 gtattgacca gccggttgcc gattttcttc ccgaattccg tcaggatggc cgggataaga
     2161 ttaccattaa gcatctactt accatgagct cggggctaag ctgggacgag tcgtacagca
     2221 ccttattttc tttaaccaca aagggttact atggaaacaa ccttgcagga ctggttcttg
     2281 gacagcacgt ggttgaggag cccggtaaac ggtttatgta ccgtagcggc gacacccagc
     2341 tgctatcgtt ggttgtagaa aaggcttcag gggaaactct ttctgactat gcttcaaaac
     2401 gtttgtggtc gaaaatcggt gcagagcacg atgcactctg gtgcctcgac aggcaaaacg
     2461 gtgttgagaa ggccttttgc tgtttcaact cgaatgcccg cgattttgcc cgctttggcc
     2521 aactcgtgct caaccagggg acttggaatg gagagcagct aatcccaaag gactatctag
     2581 cagaagcgct acaacccgcc acctatctga ttgatgccga aaccggaaaa ccctgcgatt
     2641 tttacggcta ccagtggtgg cgtattaaca tggatgggca tgaggtaaac tacgcccggg
     2701 gtttgctggg tcagtacata tttgtaatcc ccagccttaa tgcggtggtt gttcgcctag
     2761 gtcacctccg aggcgacgaa acaatcgaag ggcaacccga ggatgtttac caataccttc
     2821 gttgcggagt tgaaatcatc aagcaattac aaataccaag cgagtatgag cgaaaatgac
     2881 aacctgttcc tgactttgga acggctaagg gttaagcttc tggcctttcc tgcagctgac
     2941 gtaacgctat ttgaaaaggc taagaaaaag catatatccc tttttaaccc cgagcaaatt
     3001 gagtttgtga atgacaaacc cgatatttta atgtttctta ccggaggttc tgagcatatt
     3061 gctattgaat cggttcagga gtaccggttt tacctcatgc tggcttcacg ggaggccaac
     3121 gcctgggcgt cggcaacgga ggttaaggcg tggatgaaca tgcataatgt ttcatccttg
     3181 cttataaaca ccgatgaccc gctagcttca acctacatcc aggagttcta caaggcatca
     3241 aatggaatac gtcatttaca cgggcagcgt cttggagtag ttggcaagcc gtcagcatgg
     3301 cttgttgctt ctacgataag tcctttttta cttcagagca ggctgggtat agagcaggtt
     3361 gacatttcgt ggaatgaaat catgttcgat gaaattcagc aggtgtcgcc ggattttaca
     3421 actctgtttg gcatggctga tgacaaggag gagctcatgg ccagcggaag aatattcgag
     3481 gggttggctt cccttatccc cttttatagg cttaatgccc tttcggttga gtgctttccg
     3541 ctggtaaacc aaaccagcca cacagcatgt ttagcccttg ccaagcttaa tcttgatgga
     3601 tttcctacgg catgtgaggc cgatagctgt agtgctgctg gaatgatgct ggctaacgag
     3661 gtgtgcgggg taatcccctg gatggccaat accattttgg tcg
//
LOCUS       DAOOQX010000128         3679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_29959_length_3679_cov_3.400662, whole genome shotgun sequence.
ACCESSION   DAOOQX010000128 DAOOQX010000000
VERSION     DAOOQX010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29959_length_3679_cov_3.400662"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PLX53_02980"
     CDS             <1..614
                     /locus_tag="PLX53_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72073.1"
                     /translation="WSGYFLFIDYPILPQISQKSVDLFEQKIPTMNTAVKIACYTVLL
                     LVAGNSCNSVSKVTTTNQGISKQEFLKAPFGHDESIQAFTTQLPARTKITRFARSNAH
                     EPSRTDTIYKFSYKKSELFVYKTYFNREMLMAGKIVNPEIKLVNGISVGLTRDQLLKA
                     ISNINESSSDTLVISNEYYGRTFNFIFNNKGKVREIVFSSYAD"
     gene            complement(620..1267)
                     /locus_tag="PLX53_02985"
     CDS             complement(620..1267)
                     /locus_tag="PLX53_02985"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOY72074.1"
                     /translation="MKIHREGLVFLAVSLIILAALNVAVGLILPEVTYFLAPLSLLIL
                     LFLVRFFRIPMRGFSPNNEYIYSPADGTVVAIEEVEENEYLKTRCIQVSVFMSIWNVH
                     INWFPTTGTIKYYKYHPGDYLVAWHPKSSSHNERTTVVVERSDGTKILIRQIAGALAR
                     RIVCYAKPGQSIEQGSELGFIKFGSRVDLFLPLNANVEVKLNDRVVGKQTVIARL"
     gene            complement(1280..2107)
                     /locus_tag="PLX53_02990"
     CDS             complement(1280..2107)
                     /locus_tag="PLX53_02990"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOY72075.1"
                     /translation="MTEFLKRTISGLIFVVTITGALVIGHVAFFIVFLGLMVATQYEF
                     YKMTFKTHLRPQILLGLFIGIALFSWSFLYSLGKVEQITIFGFIPLLGGIFVVELYRN
                     QHKPIQNIAVTLLGIIYIAVPLSLVNFIVINGSSTRISYSPNILLGLLFLIWSNDTGA
                     YLLGVSMGKHKLFPRISPKKTWEGFVGGLLLTMIVSWVDAIIFPEVAFKHWLVIGVIT
                     AIVGAFGDLVESMFKRSVGVKDSGKFLPSHGGFLDRFDAFLLVIPFVYAYLEVMMLI"
     gene            complement(2104..2307)
                     /locus_tag="PLX53_02995"
     CDS             complement(2104..2307)
                     /locus_tag="PLX53_02995"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOY72076.1"
                     /translation="MDDSWIKVFSTSLPWQAEMAKQVLEENGIEAVVINQQDSSFFFG
                     EAMVYVEGKWEDQAKELLKGIGS"
     gene            complement(2300..>3679)
                     /gene="ftsH"
                     /locus_tag="PLX53_03000"
     CDS             complement(2300..>3679)
                     /gene="ftsH"
                     /locus_tag="PLX53_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOY72077.1"
                     /translation="KVEVMEIVDFLKNPKKYTELGGKIPKGVLLVGPPGTGKTLLAKA
                     VAGEANVPFFSMSGSDFVEMFVGVGASRVRDLFKQAKEKSPCIIFIDEIDAIGRARGR
                     NANFSGNDERENTLNQLLTEMDGFASNQGVIIIAATNRADVLDKALLRAGRFDRQIHV
                     ELPDYKERLDIFKVHLRPLKLEGNVDITFLAKQTPGFSGADIANVCNEAALIAARKNK
                     KQVERQDFLDAIDRIIGGLEKKNKIISKDEKRAIAYHEAGHATVSWLLEYANPLVKVS
                     IIPRGISLGAAWYLPEERQITTTEQLLDEMCATLGGRAAEELVFGKISTGALNDLERV
                     TKQAYAMITYFGMGDNFRNISYYDSTGQSEFTFSKPYSEKTAEQIDEEVKAIIDKAYN
                     RAKEILRENSDGHVKLAELLLEREVIFSEDLEKIFGPRKSLSREEELAKEFSDELKKT
                     QSPNESDNG"
BASE COUNT         1042 a          905 c          793 g          939 t
ORIGIN      
        1 gttggagtgg ttactttctt tttatagatt acccaatttt gccacaaatt tcacaaaaaa
       61 gtgtagattt gtttgagcaa aaaattccaa ccatgaatac cgcagtaaaa atagcgtgct
      121 acacagtttt actcctcgtt gcaggcaact catgtaacag cgtgagcaag gtaaccacaa
      181 ccaatcaggg cattagcaag caggaattcc tcaaggcacc atttggccac gacgaaagta
      241 ttcaagcatt caccacccag cttccagcgc gaacaaaaat aacccgattt gctcgtagca
      301 atgctcatga gcctagcaga acagacacca tttacaagtt tagctacaag aaaagtgagc
      361 tgtttgttta caaaacctac tttaatcggg aaatgctcat ggccgggaaa attgtaaatc
      421 cggaaattaa gctggtaaac ggaatctcgg ttgggcttac ccgcgaccag ctgcttaagg
      481 caatcagcaa cattaatgag tcaagcagcg ataccctggt gatttcgaac gagtactatg
      541 gtagaacgtt caactttatc tttaacaaca agggaaaggt aagggagata gttttctcga
      601 gctatgccga ttaaacctgc taaagtcggg caattaccgt ttgcttgccc acaactctat
      661 cgttcaactt cacctcaaca tttgcattta agggtaggaa aaggtcaacc cttgagccaa
      721 acttgataaa ccccaactcc gagccctgct ctattgattg gcccggtttt gcgtagcaca
      781 caatccgacg tgctaacgcc cctgcaatct gcctgatgag aattttggtt ccatcgctac
      841 gttcaactac tacggtagtt cgttcgttgt gagacgatga ttttggatgc catgccacca
      901 ggtagtcgcc gggatggtac ttatagtatt taatggtgcc agtggtgggg aaccagttga
      961 tgtgaacatt ccaaattgac atgaataccg aaacctgaat gcaacgggtt ttaaggtact
     1021 cattctcttc aacctcctca atagccacta cagttccatc agctggggag taaatatact
     1081 cgttgttggg cgaaaagcca cgcatgggaa tcctgaaaaa tctgaccaga aacagcagta
     1141 tcagtaacga aaggggagct aaaaagtatg taacttcagg taaaataagg ccaacagcta
     1201 cgtttaacgc cgccaaaata ataaggctta cagccagaaa aacgagtccc tcccggtgta
     1261 tcttcatggg taagcgtaat taaattaaca tcattacctc aagataggcg taaacaaaag
     1321 gaataaccag cagaaaggca tcaaacctat cgagaaatcc gccatggctt ggaagaaact
     1381 taccagaatc cttcactcca acacttcgct tgaacattga ttccaccaaa tcgccaaaag
     1441 caccaactat agctgtgatg accccaatca caagccaatg tttaaaggct acctctggga
     1501 aaattattgc atctacccac gacacaatca tggtgaggag cagcccgccg acaaaaccct
     1561 cccaagtttt tttaggcgaa atacgaggaa atagtttgtg ttttcccatg ctcacgccca
     1621 gcaggtaggc tccggtatcg ttgctccaaa taagaaaaag taatcccagc aaaatgtttg
     1681 ggctgtagga aattcttgtg gaggaaccat ttattacaat aaagtttacc aggctaagcg
     1741 ggacagcaat ataaataata cccagcaggg ttacggcaat attctgaatt ggtttatgct
     1801 ggttacggta gagctccact acaaagatgc cgcccagtag cggaatgaaa ccaaaaatgg
     1861 taatttgttc aacctttccg agggagtaca gaaatgacca tgaaaacagg gcgatgccta
     1921 taaaaagtcc aagaagtatc tgaggccgca gatgggtttt gaaagtcatt ttatagaatt
     1981 cgtactgagt agctaccatt agcccgagga aaacaataaa aaaagcaaca tgacctatta
     2041 ccaatgctcc ggtaatagta acaacaaaga ttaacccgct gatggtgcgt tttaagaatt
     2101 cagtcaacta ccaatacctt ttaacagttc ttttgcttga tcctcccatt ttccctcaac
     2161 gtaaaccatg gcctcaccaa aaaaaaagct ggaatcctgc tggtttataa caaccgcttc
     2221 aattccattc tcctcaagca cctgcttggc catttcggct tgccagggta gagaagttga
     2281 aaaaaccttt atccagctat catccattat cgctctcgtt tggcgattgg gttttcttta
     2341 gctcatcgct aaattctttt gccagctcct cctcgcggga gaggctttta cgggggccaa
     2401 aaattttttc caaatcttcg ctgaatatca cctcacgttc aagaagcagc tcggcaagct
     2461 tcacgtgccc atcgctattc tccctaagta tttcctttgc cctgttgtag gctttatcaa
     2521 ttatcgcctt cacctcctca tcaatttgct cggcggtttt ttcgctgtag ggtttactaa
     2581 aggtgaattc ggactgaccg gtagagtcgt agtagctgat attacggaag ttatcaccca
     2641 taccgaagta ggtaatcatg gcgtaggcct gcttggtaac acgctcaagg tcgttgagcg
     2701 ctccggtaga gattttccca aaaacaagct cctctgcagc tcttccgccc agcgttgcgc
     2761 acatttcatc cagcagctgc tcggtggtag taatttgtcg ttcctcgggc aagtaccatg
     2821 cagcccccag cgaaatacca cggggtatga tggaaacctt gaccagcggg ttggcatact
     2881 ccagcagcca gctaacggtg gcatgaccag cctcatggta ggcaatggcg cgcttttcat
     2941 ccttagaaat aatcttattc tttttctcca acccaccaat tatacggtcg atagcatcga
     3001 ggaaatcctg ccgctccacc tgctttttgt tttttcgggc agcaatcagg gccgcttcgt
     3061 tacatacgtt ggcaatatct gcccctgaaa atccgggtgt ctgcttggcc aaaaaggtga
     3121 tatccacgtt gccctctagc ttgaggggac ggaggtgcac cttgaaaata tcgaggcgct
     3181 ccttgtagtc gggcagctca acgtgaattt ggcggtcaaa ccgtccagct ctgagtaaag
     3241 ctttgtccag cacgtcggcg cgattggtgg ccgcaatgat gattacaccc tggttagagg
     3301 caaaaccatc catctcggtt agcagctggt tgagggtatt ctcgcgctcg tcgttgcccg
     3361 aaaagttggc atttcggcca cgtgcacggc caatggcatc aatttcatca ataaagatga
     3421 tacagggtga tttttccttg gcctgcttga agaggtcgcg cacccttgaa gcgcccaccc
     3481 caacaaacat ctccacaaaa tcggaacccg acatgctgaa aaaggggaca ttagcctcgc
     3541 cggctaccgc tttggcaagg agggttttac cggttcccgg agggccaacc agcagaactc
     3601 ccttgggtat cttgcctccc aactcggtgt acttctttgg attttttaaa aaatccacaa
     3661 tctccatcac ctcaacctt
//
LOCUS       DAOOQX010000129         3670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_30047_length_3670_cov_4.134440, whole genome shotgun sequence.
ACCESSION   DAOOQX010000129 DAOOQX010000000
VERSION     DAOOQX010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30047_length_3670_cov_4.134440"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..391)
                     /locus_tag="PLX53_03005"
     CDS             complement(86..391)
                     /locus_tag="PLX53_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72078.1"
                     /translation="MKMIYCTCNVAVLESLLTMLDENNVRDFQVVEQVTAKNKKGNHR
                     FNNPVWPGYNSTVFIQIAEDEKAKMVMQKIREFNQKAFNDNELVTACMWTIEDYFFE"
     gene            complement(432..3560)
                     /locus_tag="PLX53_03010"
     CDS             complement(432..3560)
                     /locus_tag="PLX53_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOY72079.1"
                     /translation="MKLPEVAVKRPVAITMLFIAVLLFGVVSLTRLPLDIMPDMELPT
                     LTVITVYPGASASEVEQQITKELEMILSGTENLKNISSQSKENVSFVQLQFDWGADIT
                     SAANNVRDLIELVKNKLPREAQNPTIFKVNSSLMPVLAYSIGAKESYGALNKLVNDEI
                     ASKLRKVSGVGSVIIIGEPEREIKINVNPLKLNAYNLSMSQIANILQASNLSIPGGNI
                     KIGSSDYAVQIPGDVASIDELAEMPLTSFNGKVIKLSDVADIHDEFKEKDEYPRTSSG
                     KGLIMMIQKQSGENTLNVANAIRLKVDELKKTLPADVTMSEVMKSDELVSESISNLSE
                     TLWYALFFVILVVLAFLRNWNQSFIIFITIPFSLIVAFIVMFAAGWTINIFSLMSLIV
                     VSGMVVDDAIVVLENITRHVENGEKPKQAAIFGTSEMGLAISASTFTTIVVFLPMVFM
                     GGIVGIMFKQLAILTSVTLLASLFTALTLTPMASSLLVKGIKPGEVHRHNRFFTASEK
                     LLTGLEGWYKKALAWVVYHKAITVIIVTIILGLSVWMGRNMGTDYIPEFDAGDVVAVV
                     ETEVGTAAAKTDSVANVVLKIFEEEIPEMAPGSIFSIAGQTEDGILSSVGFSEGKNVA
                     TIMCHLRKPNERSASAREIANRLRPRISEIPEIENFTLLGGSVLTTALTGNKKPIEIE
                     ISGNNFSTINQTAEALTAKLKQSDYFTNVDNDVDKGKLEVQVKIDKQKASAMGLNAAM
                     IGLQVRQSIYGTEAGEYKEDGDKFDITLRYSPSYRNDINQIRNIQLKNLLNQDIPLYA
                     VAQIETGTSPLQINRKEQQRLVKVMAELNGVSLGDGQMEAQRIVSSTSFPNDVDVKIA
                     GQTSDQGESFADLYLILILGILLVFMVMAAQFESFKDPFIIMFAIPVTFVGVVWAFKL
                     TGLTLSIVTFVGMIMLVGIVVKNGIVLVDYTNLLRKRGYSLYEAIQEAGRSRLRPVLM
                     TTLTTILGMVPMALSKGMGREAYSPLGVTMIGGLLISTLITLFLVPAIYGIFHNSERK
                     SNGVAKKIDYSR"
BASE COUNT          925 a          874 c          838 g         1033 t
ORIGIN      
        1 gtctgccttg caggaattgt tttttctctg tgcttcccgc atgttcgccg tagaagtgcc
       61 aaagttaatg gattgctgta tgcttttact cgaaaaagta atcctcaata gtccacatgc
      121 aggcagtaac cagctcgtta tcgttaaatg ctttttggtt gaactcccgt attttttgca
      181 tcaccatttt tgccttttca tcctctgcaa tctggataaa aactgtagag ttatagccag
      241 gccaaacggg gttgttgaac cggtggttac cctttttgtt ttttgccgta acctgttcca
      301 ccacctggaa gtcgcgcacg ttgttttcgt ccagcatggt tagtagcgat tccaaaacgg
      361 caacgttaca ggtgcagtaa atcattttca tgcgtttgca gctattagtg aaattaacat
      421 gaagctataa actatctgga atagtcaatt tttttagcta ctccatttga ttttctttct
      481 gaattgtgga aaattccata gatagccgga acaaggaaca gggtaatcag cgttgaaatc
      541 agcagaccgc caatcatggt aacgccaagg ggtgagtaag cctcgcgccc catacccttg
      601 ctcaaggcca tgggcaccat tccaaggatt gtggttaagg tagtcatcag cacgggtcgc
      661 aacctgctac ggccggcttc ctgtatggcc tcgtagaggc tatagccacg cttacgcagg
      721 aggtttgtgt agtcaaccag cacaatacca ttcttaacca caatccctac cagcataatc
      781 atgccaacaa aagtaacaat actaagggtt aagcctgtaa gcttgaaagc ccacacaaca
      841 ccaacaaagg taacaggaat ggcgaacata atgatgaatg gatccttgaa gctttcaaat
      901 tgggcggcca ttaccatgaa aaccagcagg atgccaagga tgagtatcag gtaaaggtcg
      961 gcaaacgact cgccctggtc ggaggtttgt ccggctatct ttacatccac atcattcggg
     1021 aatgaggttg atgaaacaat acgctgtgcc tccatctgcc catctccaag cgaaacccca
     1081 tttagctcgg ccatcacctt aaccagccgc tgctgctcct tacggttaat ctgcagcgga
     1141 cttgttccgg tttcaatctg ggcaacagca taaagcggaa tgtcctgatt aagcaggttt
     1201 ttcagctgga tattcctgat ttggttgatg tcgttccggt agctgggcga gtagcgaagc
     1261 gtaatgtcga acttatcgcc atcctcctta tattcacctg cttcggttcc gtaaatgctt
     1321 tgacgtacct gaagcccaat catagctgcg ttaagcccca tggccgaggc cttctgtttg
     1381 tctattttaa cttgaacttc gagtttcccc ttatccacat cgttgtccac attggtaaag
     1441 tagtccgatt gcttcagctt ggctgttaac gcctcggcag tttggttgat ggttgaaaag
     1501 ttgttacccg agatttcgat ttcgatgggc tttttgttgc ctgtcaaggc tgtggttagc
     1561 acacttccgc ccaggagggt aaaattctca atttcaggaa tttccgaaat tcttggacgc
     1621 agacggttgg caatttcgcg agctgatgcg ctcctttcgt tcggtttacg caggtgacac
     1681 atgatagtgg ctacgttttt cccctcgcta aatcctactg acgagagaat tccatcctcg
     1741 gtttggccgg caatagaaaa aattgagcct ggagccatct cgggaatttc ctcctcaaag
     1801 atttttaaaa ccacgttggc tacactatcc gtttttgctg cagcggttcc tacctcagtt
     1861 tcaaccacgg ccactacgtc gccggcatca aattcgggaa tatagtcggt tcccatattt
     1921 ctgcccatcc aaacggaaag accaagaatg atggtaacaa tgataacggt aatggccttg
     1981 tgataaacta cccacgctaa agcctttttg taccagcctt ccagcccggt tagaagtttc
     2041 tcgctggcag taaagaacct gttgtgccta tgtacctctc ccggtttaat gccttttacc
     2101 aagagcgatg aggccatagg ggtgagcgta agagccgtga agagggaagc caaaagggtt
     2161 accgaggtga gaatggccag ctgcttgaac atgattccta caattcctcc catgaaaacc
     2221 atcggcagaa aaacaacaat ggtggtaaaa gttgaggctg atatggccaa acccatttcg
     2281 ctggtaccaa atatggccgc ctgtttaggc ttttcgccgt tttcaacatg tctggtaatg
     2341 ttttcaagca ctacaatggc atcgtcaacc accattcctg aaacaactat aagcgacatc
     2401 aggctaaaaa tgttgattgt ccatcccgcg gcaaacatta cgatgaaggc tacaattagc
     2461 gaaaagggaa tggtaataaa aatgatgaag ctctggttcc agtttcgaag gaacgccagc
     2521 acaaccaaaa tgacgaagaa aagggcgtac caaagcgttt cggataggtt gctaatagac
     2581 tccgagacca gctcatcgct cttcatcacc tcgctcatgg taacatctgc aggcagtgtc
     2641 ttttttagct catctacctt aagcctgata gcgttggcca cattaagcgt attttcgccg
     2701 ctctgctttt gaatcatcat aataagccct ttaccgctgg aggttcgagg gtactcgtcc
     2761 ttctccttaa actcatcgtg gatgtctgcc acgtcgctta gcttgataac cttaccgttg
     2821 aagctggtca ggggcatttc cgccaactcg tcaatgctgg caacatcgcc cggaatctga
     2881 acggcataat cgctgctgcc aatcttgatg tttcctccgg gtattgagag attggaagcc
     2941 tgaaggatgt tggcgatttg cgacatggag aggttatagg cgttgagttt aagggggttt
     3001 acgttgatct tgatttcgcg ttcaggttcc ccaatgataa ttactgatcc aacacccgaa
     3061 actttgcgca gcttgctggc tatttcgtcg tttaccaact tgttgagtgc accatagctt
     3121 tcctttgccc caatgctgta agccaataca ggcattagcg agctgtttac cttgaaaata
     3181 gttggattct gggcttcgcg gggcaacttg tttttaacca gctctatcag gtcgcgaaca
     3241 ttattggctg ccgaggtaat atctgcaccc cagtcgaatt ggagctggac aaacgaaacg
     3301 ttctccttgc tttgcgagga gatgtttttc aggttctctg tgcctgaaag tatcatctcc
     3361 agctctttgg taatctgctg ctcaacttcg ctagcgctag cgcccgggta aactgtgatt
     3421 actgttaggg tgggaagttc catatcgggc atgatgtcga gcggtagccg ggtgagggat
     3481 accactccaa acagcaaaac agctataaaa agcattgtaa tggctactgg ccttttgacc
     3541 gctacttctg gcaatttcat ccgtgcaaaa ttttaatatt gtgagttggc aataaactac
     3601 aatttggcat tttatactcg ttaacgttcg tgtagaacat ccattgcttt gaaatccttt
     3661 gtgctacatg
//
LOCUS       DAOOQX010000130         3650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_30278_length_3650_cov_3.176078, whole genome shotgun sequence.
ACCESSION   DAOOQX010000130 DAOOQX010000000
VERSION     DAOOQX010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30278_length_3650_cov_3.176078"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1760)
                     /locus_tag="PLX53_03015"
     CDS             complement(<1..1760)
                     /locus_tag="PLX53_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72080.1"
                     /translation="MTRRKYIINWLIVVFDIWLITNSFPIYATSTANPDDSRLQQFSF
                     NDTIARIDLRISEFEKNGNRAELAKALTEKAGIYFRVARYDDALMTANQALLVANEEG
                     REQLLEPIYHLLSLIHIENELVFEAAEYLYAGYQKSLDMKDTANIGWYLVNLSEVEEY
                     LGRVSNAMEINLTAIDFFKNTSDSLSLAKIYRAQGVAHAELKNFLTAEDYFKKAFRIF
                     NSCGDSLNMGLVLMDQASLNLKELNLYEAQSLIGQAMKLLEKQSQKFYLRSRCIYGEL
                     LVQRRSFTEAIEMLKETLMIQLSINDLYGYTATLSALGRAYLGSGNRQMAIDSYTRCA
                     KMADKANLINFKRDSYRNLASILGSEGNSSKAYYYLSSYVTLTDSLFNLQKISEANRL
                     ENQAMLRMKEKEIAQQKEQIILNSSQLRQESVKQKFLYIIIILSFGVTAAVVYSYRQK
                     KTANALLTEQNNRIEKQRDMLEFRSREIMDSLNYAQRIQRAILHSSLQPEDFFPDSFL
                     IFIPKEVVSGDFYWFKAVDNLKLFAIADCTGHGAPGAFMSIIGMFGLNQIITEYGQTH
                     PGDVLNQLNELFYHSFEQREG"
     gene            complement(1757..1990)
                     /locus_tag="PLX53_03020"
     CDS             complement(1757..1990)
                     /locus_tag="PLX53_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72081.1"
                     /translation="MGMAFIFSAKVAEYAHIHQPVVKNYIQKVGSSSKKLNDKLKRCH
                     HVEMAQKRILGKSKNIPDKKLPSHIENLISNLG"
     gene            1974..3035
                     /locus_tag="PLX53_03025"
     CDS             1974..3035
                     /locus_tag="PLX53_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOY72082.1"
                     /translation="MKAIPIYLSLLLALFSCGNRAESRGGSILEMNANYPPKYAEGFT
                     IFNDSTGTKIRVFNPWQGASNVSLEYKLVPRGDISKFSSNPGAIPVPLERVVCMSSSH
                     LAFIEALGMGQRIVGVSGAGFAFSSALRAMVDEGKIFEVGYDQGINYEQIARLNPDVI
                     LCYGIGSESMAYLKKFSEMGMRIMFIGDYLESDPLGKAEWIKVLGCLFNCYGKADSIF
                     SSIESSYLKTKKQVERCSSKPTIFMNMPFRGVWYFPGNDSYMVKLVHDAGGSYVFENL
                     EGSRSFPLGIEKAFEAGMGSDIWLNPGSASSLTDITSFDTRLAQLPPVVSGKVYNNNL
                     RMSRGGVTTFGNREQFIPS"
     gene            3168..>3650
                     /locus_tag="PLX53_03030"
     CDS             3168..>3650
                     /locus_tag="PLX53_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOY72083.1"
                     /translation="MERSSKNYGKSKERKPTGKVGMMVVLLAVLVFLLVFIDLLLGSV
                     RIPMSDVLKFIFTGSAGSEVSQNILFNFRLPRVLAAVLSGSALAVSGLLMQTIFRNPL
                     ADPSVLGISAGASLGVALVVMGSSALGLSFLGNQFSMVLAALAGSMLVLMLVMAVSVR
                     V"
BASE COUNT          889 a          775 c          914 g         1072 t
ORIGIN      
        1 ccctcacgct gttcaaacga gtggtaaaag agctcgttta gctggttgag cacgtctccc
       61 ggatgtgttt gcccgtactc ggtaataatc tggttgagcc caaacatccc aataatactc
      121 ataaatgccc ctggcgctcc atgtccagta cagtctgcaa tggcaaacag cttcaagtta
      181 tcaaccgctt taaaccagta aaaatcgcca ctaaccacct cctttggaat aaaaatcagg
      241 aacgaatcgg gaaaaaaatc ttcgggttga agcgatgaat ggagtatagc ccgctgaata
      301 cgctgggcat agttgaggct atccataatt tcgcgtgacc tgaattccag catatccctt
      361 tgcttttcaa tcctgttgtt ttgctcggtt aacagggcat ttgcagtctt tttttgtcga
      421 tagctgtaaa caacggcagc tgttactcca aacgaaagga taatgatgat gtaaagaaat
      481 ttttgcttca cgctttcctg ccttagctgc gagctattca aaatgatttg ctccttttgc
      541 tgggcaatct ccttctcctt cattctcagc atcgcttggt tctccaaccg gtttgcttcg
      601 ctaatctttt gtagattgaa aaggctatcg gttagggtta cgtagctcga taggtagtag
      661 tatgccttac tgctgttccc ctcggagcca agtatggacg caaggttgcg gtatgagtct
      721 cttttaaagt taatgaggtt ggccttgtcg gccatcttag cgcagcgggt atagctgtca
      781 atagccattt ggcgattgcc cgaacccagg tatgctcttc cgagtgccga tagggtggcg
      841 gtgtacccgt aaaggtcgtt tattgagagc tgaatcatga gagtctcctt cagcatctca
      901 atggcttcgg tgaagctccg tcgttgaaca agcagctcgc cgtagatgca cctgctgcgg
      961 aggtagaatt tttgactttg cttttcaagc agcttcattg cctgcccaat aaggctttga
     1021 gcctcgtaaa ggtttagctc tttgaggttg aggcttgcct gatccatcag aaccaacccc
     1081 atgttaagcg aatcgccaca gctgttgaat attcgaaagg cctttttaaa gtaatcctcg
     1141 gcggttagga agtttttcag ctcggcatgt gctactcctt gagcacggta tattttggcc
     1201 aggcttagcg agtcactggt gtttttaaag aaatcaattg cagtcaggtt aatctccata
     1261 gcgttagaaa cccggcccag gtactcctca acttcactta ggttaaccaa gtaccagcca
     1321 atatttgcgg tatccttcat gtccaggctt ttctggtagc cagcataaag atattcggct
     1381 gcttcaaaca ccagctcatt ttcaatgtgg ataagggata acagatggta tattggttca
     1441 aggagttgtt cacgtccttc ctcgttggcc acaagcaagg cctggttggc cgtcattagt
     1501 gcatcgtcat agcgggctac tctaaaatag atgcccgcct tttccgttag tgccttggca
     1561 agttcggccc tgttaccgtt tttttcgaat tcgcttatcc gcaaatcaat cctggcgata
     1621 gtatcattaa aagaaaactg ttgcaatcgg ctatcgtcag ggttggcggt tgacgtagca
     1681 taaatgggaa atgagttggt aatcaaccat atatcgaaaa caactataag ccaatttatt
     1741 atgtatttcc gcctagtcat cctaaatttg aaatcaaatt ttcaatgtgt gaaggtagct
     1801 tcttatcagg aatattctta ctcttaccca gaattctttt ttgtgccatt tcaacatgat
     1861 ggcagcgctt tagcttatca ttcagctttt ttgagctgct gcctactttc tggatgtaat
     1921 ttttcacaac cggttggtgg atatgggcat attcagctac tttagcggaa aaaatgaagg
     1981 ccatacccat atacctatcc ctgttgctcg ccctgttttc atgtggcaat cgtgcggaga
     2041 gcagaggtgg ctcaatcttg gagatgaacg ccaattatcc gccaaagtat gccgagggtt
     2101 ttactatttt caacgatagc accggcacta aaatacgggt tttcaacccc tggcaaggtg
     2161 ccagcaatgt tagcctggag tataagctgg ttccaagggg ggatattagt aaatttagct
     2221 ccaaccctgg tgcaattccg gttccgctgg aacgtgttgt ttgcatgtcg tccagccatt
     2281 tggcatttat tgaggcgttg ggaatgggac agcgaattgt gggagtttcc ggtgcggggt
     2341 ttgctttcag ctccgcgctg agggcaatgg ttgatgaggg aaaaatattc gaggttggct
     2401 atgatcaggg gataaactac gagcagattg cccggttgaa tccagatgta attctttgct
     2461 acggaattgg ttccgaatca atggcatacc ttaaaaagtt tagcgaaatg gggatgcgca
     2521 ttatgtttat aggcgactac ctggagtccg atccgcttgg taaggctgaa tggattaagg
     2581 ttttaggctg tctttttaat tgctacggca aagccgattc gatttttagt agcattgaaa
     2641 gtagctacct gaaaaccaaa aagcaggttg agcgttgcag ctcaaaacca accattttta
     2701 tgaacatgcc ctttcggggt gtgtggtatt tcccgggtaa tgacagctac atggtgaagc
     2761 tggtgcatga tgccggaggc agctatgttt ttgagaacct ggagggtagc cgaagcttcc
     2821 cactgggaat agaaaaagca tttgaggccg gaatgggcag tgatatctgg ctaaacccgg
     2881 gttcagcaag cagcttgacg gatatcacct cgtttgatac gcgcttagcc cagctgccac
     2941 cagtagtttc tggcaaggtg tataacaata acctccggat gagccggggg ggggtaacga
     3001 cttttgggaa tcgggaacaa ttcatcccga gttgatcctg cacgacctta tttccatttt
     3061 ccaccccgaa attgcgaacg attcactttt ggtttactac cagaagttgg agtagcttaa
     3121 caactaacga gattaccatt gattttggcg taagctgagt aataataatg gagcggagca
     3181 gtaaaaacta cggtaaaagt aaggaaagaa agcctacagg gaaggtaggc atgatggtag
     3241 ttttgcttgc tgtgctggtt tttttactgg tttttattga tttactgttg gggtcggtta
     3301 gaattcccat gagtgacgtg ctaaagttta tatttaccgg aagcgcaggc agcgaggtaa
     3361 gccaaaatat actgtttaac ttcaggttgc ccagagttct ggcagctgtg ctgtcgggtt
     3421 cggctctggc ggtaagcggc ttgctgatgc aaaccatatt tcgaaatccc ttggccgacc
     3481 cttcggtgct gggaattagt gctggtgcca gcttgggggt tgctttggtg gtgatgggtt
     3541 catcggcgtt agggctttca tttttgggaa atcaatttag tatggtgctg gccgcattgg
     3601 caggttcaat gcttgtgctc atgctggtaa tggccgtttc ggtgagggtg
//
LOCUS       DAOOQX010000131         3625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_30563_length_3625_cov_2.672269, whole genome shotgun sequence.
ACCESSION   DAOOQX010000131 DAOOQX010000000
VERSION     DAOOQX010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30563_length_3625_cov_2.672269"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..421)
                     /locus_tag="PLX53_03035"
     CDS             complement(125..421)
                     /locus_tag="PLX53_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72084.1"
                     /translation="MPSWQGRRYFSPAMVNHGIPKEDSPIAVMLNEHQLGRGHIKSLS
                     SAFEEFKDGNKLAINNIISSSMNYVELVCRLAPTVGFEVNNQVAINPSAFDTVH"
     gene            complement(789..2246)
                     /locus_tag="PLX53_03040"
     CDS             complement(789..2246)
                     /locus_tag="PLX53_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72085.1"
                     /translation="MKTKRELNYLGLIFILIATLSSIYSCQKNDSSEITADVIISSSS
                     VNNPVIIDTIVQQDYPLHIITGQKEFDWENAEFIPLPSTQNPIPVPWSDRARRNFSED
                     IRYDYKKEDGWLMYYCSFSDELNPPVKTFMLYNKYRGILRFYYFFTGTGIENVLDYNI
                     FSNDIMSMGSHASMSPILNFAHQNIIDVNKNSTHCVTIEPEILSDSTWFAWEYELAFD
                     KDIYNQDPNTFLLNIGYGMLKKIALSINGIQLDDLNARIRFTDGTYQYGDSYNGNANI
                     IIYGKNDINQMSNILSGSDLTLINQIYDQQSFNNILNGSVNYNYLGEIQWNANLAITT
                     QPNGVGLPGGGQSFVISGANNYVMQGLGSFYNKPLGIFYLNRKPKVSYSKINSGNHQF
                     QYELNVNSVEYIINPSVLDFADIKNISQNLVATENKELIENNSRAKLYIGQKLLSNIE
                     LTIQGVRVSFDVVPKNGSKPVHIVKVFQADIETSK"
     gene            complement(2626..>3625)
                     /locus_tag="PLX53_03045"
     CDS             complement(2626..>3625)
                     /locus_tag="PLX53_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72086.1"
                     /translation="WGNVGVVKDNIQWGSGYNGSNILGGRTPSFAMIKLNITPARWVE
                     LNYLHGWLVSEVIDSSRTYTSGDEVRKVYHPKYIAANLIAFKPFRGLSLSVGNSIIYS
                     DINVQPAYLIPFLFYKSIDHHLNGMSNSVGQNSQMFFDISSRNIRHLHLYATLFLDEI
                     SMKNMFNPDMHSNFYSLKTGVRISDFPFSNLIVTGEYTRTNPLTYRHIINTTTYESNN
                     FNLGHYLQDNADEIYVSLLFRPIRGLQVSMSYTVSRKGPDYSTITTTRLGLPFMESVE
                     WELREWAVGAKYEVINDGYIYLSGGVSDITDRVGNYTPDFMVGRQAQLRAGVCFGF"
BASE COUNT         1032 a          746 c          677 g         1170 t
ORIGIN      
        1 gccctcaccg tcgcgctaat ggccacaccg ggctccccac gagcgggcgg attggttgca
       61 aatctgggcg agtgtggctt cttaagatac ataatccttc cgacgcgtag gaattaacgt
      121 agtgtcaatg aacagtatcg aatgccgacg ggtttatggc aacctggtta ttgacctcaa
      181 atcctactgt tggagcaagc ctacatacaa gctcaacata attcatcgaa cttgagataa
      241 tgttattgat tgcaagtttg ttcccatctt tgaattcttc gaatgcgcta ctcaatgatt
      301 ttatatgacc tctgcccaac tgatgctcat taagcattac tgctattggg ctgtcttctt
      361 ttggaatgcc atgatttacc atagcagggg aaaaatatct tcttccttgc catgatggca
      421 tgaatcagca aaacctttta taaactcaat gattttagca taatgctcct tattcatatc
      481 cttgcctctt tcaaaattat ctgaaatttt tccatgatag aaagcataat ttttatacct
      541 tcatgttcat cttttaattg ttgtgtagct ttcatcttaa tttctccttt tagtatcatt
      601 gattttcttt atcatcctta ataattgtca gttttcttag catgaccgct aacggcggcg
      661 atatgaaacg tagccgattg cgttgctaca atcctatcaa ccgataagaa ccttgccagc
      721 gataaatgcg cttagcgtac cccttaaacc ccaatgtttt atgaccgctt gttgctcaca
      781 ttttttattt actttgaagt ttcaatgtct gcctgaaaaa ctttaactat gtgtacaggt
      841 ttgctaccat ttttaggaac tacatcaaag ctcacccgaa ctccctgaat agttaattcg
      901 atattggata aaagtttctg cccaatatat aattttgctc tgctgttatt ttcaattaat
      961 tccttatttt ctgtggctac taaattttga gatatatttt taatatctgc aaaatccagt
     1021 acagatggat taataatata ctcaacagaa tttacattca gttcatactg aaactgatgg
     1081 tttcctgaat taatctttga ataagatact ttaggcttac gattcaggta aaaaatacct
     1141 aagggcttat tataaaatga ccccaacccc tgcattacat aattattagc accactaatt
     1201 acaaatgatt ggcctccacc gggcagtccc actccatttg gctgagtagt tattgctaaa
     1261 ttggcattcc attgaatttc gcctaaataa ttataattta ctgaaccgtt caaaatatta
     1321 ttaaaagatt gctgatcata tatctgatta attaatgtta aatcagaacc agataaaata
     1381 ttggacattt ggttgatatc gttctttcca taaattatga tgttcgcatt tccattatat
     1441 gaatctccat attggtaagt cccatcagta aaacggattc gcgcatttaa atcatccagc
     1501 tgaattccat ttattgacaa tgctatcttt tttaacattc cataacctat gttaagaaga
     1561 aatgtattag gatcctggtt atatatgtct ttatcgaatg ctaattcata ttcccatgca
     1621 aaccaagtcg aatcggataa aatctcgggc tcaattgtaa cgcaatgtgt tgaattttta
     1681 ttaacatcaa tgatattttg gtgagcaaag tttaatatcg gagacatgct tgcatgagaa
     1741 cccatcgaca ttatatcatt gctgaatata ttataatcca aaacattctc tatgcctgtt
     1801 ccagtaaaaa aataatagaa tctcagaatg cctctgtatt tattgtatag cataaatgtt
     1861 ttaacaggcg gattcaactc atcagaaaaa gaacaataat acatcagcca accatcttct
     1921 tttttataat cataacggat atcctcgcta aaatttcttc ttgccctatc gctccatggt
     1981 acaggtatag gattttgtgt agagggtaat ggtataaatt ctgcattttc ccaatcaaat
     2041 tctttttgcc ccgtaattat atgaagtgga taatcctgct gaactatagt atcaataata
     2101 acaggattat taactgatga actactaatt attacatctg cagttatttc agatgaatcg
     2161 ttcttttgac aactataaat agacgataga gttgctatta agataaagat taatcctaga
     2221 taattcaatt ctctttttgt tttcatgatc gttggttttg ttgcgttaat tattcattat
     2281 ctccttaaaa ttaaggggtt catttaactt caattgtggg taactcattt ataggcgcca
     2341 ctctgttgcg cattatttcc atttttggat gtaaaggcgc tatgatgttg cgttctattt
     2401 ctataaacaa atgtagtgta atgttacaac tcatcaaatg ggttcctaat tttctgcaaa
     2461 tttttctgtg aagcatgggt ataaatttca gctgttgttg aactcttatg ccctcaccgt
     2521 cgcaccaatg gccacaccgg gctctctacg agcgggcgtt taggtacggg tagtatcagc
     2581 aggcaaacag cataattctc aaataatacc atagcagcca ggtgcttaaa acccaaagca
     2641 aacaccagcc ctaagctgtg cctgcctgcc caccatgaaa tcgggggtgt agtttccaac
     2701 cctgtcggta atatccgaaa caccgcctga aaggtagata tagccgtcat taatcacctc
     2761 atactttgcc cctactgccc actcccgtag ctcccattcc acgctttcca taaatggtaa
     2821 cccaagcctg gtggttgtta ttgtgctgta gtcgggcccc ttacggctga cggtgtaact
     2881 catggaaact tgaaggcctc tgataggcct aaatagcagc gatacgtaaa tttcatcggc
     2941 attgtcctgc aggtagtgcc ccaggttaaa gttgttgctt tcgtaagttg ttgtattaat
     3001 tatatgcctg tacgtcaacg ggtttgtgcg tgtatattca cctgttacga tgaggttgct
     3061 aaatggaaaa tccgaaattc tgacaccggt tttcaggctg tagaagttac tgtgcatatc
     3121 ggggttgaac atgttcttca tcgaaatttc atcgaggaaa agggtggcgt acaggtgcag
     3181 gtgtcttatg tttcgcgacg aaatgtcaaa gaacatctgg gaattttgtc ccaccgagtt
     3241 gctcatcccg ttcaggtggt ggtcaatgga cttgtagaac agaaagggga tgaggtacgc
     3301 aggctgaacg ttaatatcgg agtagataat ggagttgcca accgacaggc ttagccccct
     3361 gaagggttta aaggcaatta ggttagcggc aatatacttg gggtggtaca cctttcgcac
     3421 ctcatcacca gaggtgtagg tgcggctgga gtcgataacc tcggaaacaa gccacccgtg
     3481 caggtagttc agctcaaccc aacgtgcagg agtgatgttt agcttgatca tggcaaagga
     3541 aggtgtccgc ccgccgagaa tgttggaacc gttgtagccc gatccccact gaatgttgtc
     3601 ctttacaacg ccaacgttac cccac
//
LOCUS       DAOOQX010000132         3619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_30637_length_3619_cov_3.372615, whole genome shotgun sequence.
ACCESSION   DAOOQX010000132 DAOOQX010000000
VERSION     DAOOQX010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30637_length_3619_cov_3.372615"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3619
                     /gene="sprA"
                     /locus_tag="PLX53_03050"
     CDS             <1..>3619
                     /gene="sprA"
                     /locus_tag="PLX53_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOY72087.1"
                     /translation="RFMRMFLRGFQDTMILRFATLELVKSEWRKYNYSLIQGQEGIPS
                     GDLPTTGFAISSVNIEENDKRTPVNYVLPPGVDRVIDPSQPQLTELNEQAMEYRVIDL
                     ADGDARAAFKNVNIDIRQYKKLKMDVHAEMVEGYPLSNGDVSAFIRIGTDYTDNYYEY
                     EIPLSLTPYGYYFNNRESDREMVWPRENLINIDLEKLVEVKVARNDAMRTPGSMVTQR
                     TLYTVDDGDAQMKVVGNPNLSNVKVIMIGIRNPASGKGPTDDGMPKSVVAWFNEFRLT
                     NFNDQGGWAANARVSAKLADLGMINVAGSTVTPGFGSIEKKVNERSKETIYQYDINST
                     LELGKFFPEKLNVRIPMFLGFSESISNPQYNPLDPDVTLSSSLNSYNPSERDSVKRIV
                     QDYSRHRSINFTNVKIDKTTEKPHFWNISNFSVSYSYSELFSRNIRTDHKIQRDIRGA
                     LTYNFNKQPKYIVPFKNVKWLNSKYFRLIKDFNFNLTPAQLSFRTDLDRSYFEQQFRN
                     VSNPSAVLLPTYSKDFLWNRNYALNWDITQSLKFDFTASNVARIDEPEGMVDRNRDPK
                     GYEHWRDSVWQNIKNFGRNTMYNHQFNLSYSLPLSKIPFLDWTNASARYTGIYRWQAG
                     PIMADTSRFDPGNTVQNSNTIQLNGQININTLFNKVGYLKRINQEFDSRGRGAAPKTK
                     TVKYEEGGVNIRAKYAKNINHKLKTDKVTVRVYDEKGNEVKVKSEVKSDTRVAIRADA
                     DYSNCRVVVEGKVPDRPNPLKFAWEGTLRLMMSVKSLSLSYSETNGTLLPGYKPGTRY
                     LGIDSYNGFTAPGWEFIAGWQDPEIAWKAATNGWLTHDTTFNTPVVFTHNENLNFRSS
                     IEPVPGFRIELTANRTYSRNHNETYRANALGSYSSLSPLTTGNFSISVLTLGTTFEGK
                     NRDAKSRAFERMKSNRLVIAERLRDQRVANPGQGYDPMAPPDSLGFPVGYGPLSQDVL
                     RASFLAAYTNTSPQKVTLRDFPVIPMPNWQITYDGLAKIRPFKNLFKTFTLSHGYRSA
                     YTIGAFTTNLNYLPEDDGFSYVRNINGDFVPKREITNVSINEQFSPLLGLDMGWQNNL
                     TTRLEFKTSRSLAMSFANNQLTEIYGNEYIIGAGYRFENLPLVFQSEDGGKSAVKSDL
                     RITVDLSIRENTTILRKLVEDYSEIIAGQLVNTIKTSADYRINEQVTVRLFFDRTLNA
                     PK"
BASE COUNT          999 a          912 c          860 g          848 t
ORIGIN      
        1 cgcttcatga gaatgttcct gcggggcttt caggacacca tgatcctgag atttgccacc
       61 cttgaactcg tgaaaagcga gtggcgtaag tataactact cgcttattca ggggcaggag
      121 ggtattcctt cgggcgattt acccaccacg gggtttgcca tctcttcggt caacattgag
      181 gagaacgaca aacgcacgcc ggttaactac gtgctgcccc cgggcgttga cagggttatt
      241 gaccccagcc agcctcagct caccgagctc aacgagcagg ccatggagta cagggtgatt
      301 gacctggccg atggcgatgc cagagcggct ttcaaaaatg tcaatataga tatccggcag
      361 tacaaaaaac ttaaaatgga tgtgcacgcc gagatggttg agggttatcc gttgtcaaat
      421 ggcgatgttt cggcatttat ccgaatagga accgattata ccgacaacta ctacgagtac
      481 gaaattccct tgtccctgac accctacggc tactacttta acaaccggga gtcggatagg
      541 gaaatggttt ggcctcgtga gaatctaatc aatattgatc tggaaaagct ggtggaggtt
      601 aaggtggccc gtaacgatgc catgcgaacc ccaggctcaa tggtaaccca acgaaccctc
      661 tacacggttg atgatggtga tgcccagatg aaggtggtgg gaaatcccaa cctcagcaac
      721 gttaaggtga ttatgatagg tattagaaac ccggctagcg gaaagggtcc aaccgatgac
      781 ggaatgccta aatcagtggt ggcctggttc aacgagtttc gacttaccaa ctttaacgat
      841 caggggggat gggccgctaa tgcccgagtt tcggccaagc tggccgattt agggatgata
      901 aatgtggccg gttcaaccgt tactcccgga tttggctcta tcgagaaaaa ggttaacgag
      961 cgcagcaagg aaactattta ccagtacgat atcaactcca ccctggagct gggtaagttt
     1021 ttccccgaaa agttaaacgt aagaataccc atgttcctgg ggttcagcga gtccatatcc
     1081 aatccccagt ataaccccct tgaccccgat gtaaccctta gcagctccct gaattcctac
     1141 aacccttcgg aaagagattc cgtaaagcgg attgtgcagg attactcccg acatcgaagc
     1201 atcaacttta caaatgttaa aattgacaag actactgaaa aacctcattt ttggaacatt
     1261 tccaacttct ccgtgagcta ctcctacagc gagctgttct caaggaatat ccgcaccgat
     1321 cataagattc aacgggatat tagaggcgcc ctaacctaca acttcaacaa gcagccaaag
     1381 tacattgtgc cctttaaaaa cgtgaagtgg ttgaactcaa aatatttcag gttgattaag
     1441 gacttcaact ttaaccttac cccagctcag ctctccttcc gaaccgattt ggatcgaagt
     1501 tactttgaac agcagtttcg caacgtaagc aaccccagcg ccgtgttgct tccaacctac
     1561 tccaaggact ttctgtggaa tcgcaactat gctctgaact gggacattac ccaatcgttg
     1621 aaatttgatt ttactgccag caacgtagcc cgtattgatg aacccgaggg catggttgat
     1681 cgaaaccgcg accctaaagg atacgagcac tggcgcgact cggtgtggca gaacattaag
     1741 aattttggcc gtaataccat gtataaccac cagtttaacc tttcctactc gcttccacta
     1801 agtaagatcc catttcttga ctggactaat gcctcagcac gctacactgg aatttaccgg
     1861 tggcaggcag gccccattat ggccgataca tcgcgattcg acccaggtaa taccgttcaa
     1921 aactccaaca ccatccagct taacggtcaa atcaacatta atactctttt caacaaggtt
     1981 ggctacctta agcgaattaa ccaggagttt gatagtaggg ggcgaggggc agcacctaaa
     2041 accaaaaccg ttaagtacga ggaaggcggg gtgaacatta gagcgaagta cgcaaaaaat
     2101 atcaaccata agctcaaaac cgataaggta actgtaaggg tgtacgacga gaagggcaac
     2161 gaggtaaagg ttaaatcgga ggtcaaaagt gatacacgtg ttgccatcag ggcagatgcc
     2221 gattacagca actgcagggt ggtggttgaa ggcaaggtgc ccgataggcc caatccgctg
     2281 aagtttgcgt gggagggcac acttcgcttg atgatgagcg ttaaaagcct ttcgctttcc
     2341 tattccgaaa ccaacggaac gcttctgcct ggttacaaac ccggtacacg gtacttagga
     2401 attgacagct acaacggctt tacggctccc ggatgggagt tcattgccgg ttggcaggat
     2461 ccggaaattg catggaaagc agctaccaac ggctggctca cacacgatac cacctttaat
     2521 acgccggtgg tgtttaccca caacgaaaat ttgaacttcc gcagcagcat tgagcctgtg
     2581 cccggttttc gaattgagct aacggccaac cgaacctact cgcgcaacca taacgaaacc
     2641 tacagggcaa atgcactagg gtcgtatagc tcgcttagcc cgctgacaac cggtaatttc
     2701 agcatcagcg tactaacgct tggaactacc tttgagggaa agaaccgcga tgcaaaatcc
     2761 agggcttttg agaggatgaa gagcaaccgg ctggtaattg ccgagaggct tcgcgatcaa
     2821 cgagtggcta atcccggtca gggttacgac ccaatggctc caccagactc tttgggattc
     2881 ccggtgggtt acggtcctct atcgcaggat gtgctcaggg cttcattcct ggctgcctat
     2941 accaatacct cgccgcagaa ggttaccctt agagattttc cggttattcc aatgcccaac
     3001 tggcagatta cctacgatgg acttgctaaa attcgaccat ttaagaatct ttttaaaacc
     3061 tttacactat ctcatggcta ccgatcggcc tatactattg gcgcctttac aaccaacctg
     3121 aactatctgc ccgaggacga tggttttagc tacgtgcgca acataaatgg cgattttgtg
     3181 ccaaaacgtg aaattaccaa cgtttccata aacgagcaat tttctccatt gcttgggttg
     3241 gatatgggct ggcagaacaa cctcaccacc cgacttgagt ttaaaaccag ccgttcgctg
     3301 gcaatgagtt ttgccaacaa ccagcttacc gaaatttacg gaaacgagta tattatcggg
     3361 gcaggctaca ggttcgagaa tttgcccctt gtgtttcagt cggaggatgg cggtaagagc
     3421 gccgttaaga gcgacctgcg gattactgtt gatctctcca tacgcgaaaa cacaaccatt
     3481 ttgcgcaagc tggtggagga ttactccgaa attattgccg gacaactcgt taataccatc
     3541 aaaacctccg ccgactaccg catcaacgag caggttacgg taaggctgtt tttcgaccga
     3601 accctcaacg ccccaaaag
//
LOCUS       DAOOQX010000133         3551 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_31428_length_3551_cov_3.054920, whole genome shotgun sequence.
ACCESSION   DAOOQX010000133 DAOOQX010000000
VERSION     DAOOQX010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31428_length_3551_cov_3.054920"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2461)
                     /gene="uvrA"
                     /locus_tag="PLX53_03055"
     CDS             complement(<1..2461)
                     /gene="uvrA"
                     /locus_tag="PLX53_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOY72088.1"
                     /translation="MEFLKGEEEEVVVEGARVHNLKNIDVAIPRGKLTVITGLSGSGK
                     SSLAFDTIYAEGQRRYMETMSAYARQFLGTLERPDVDKISGLSPVISIEQKTTSHNPR
                     STVGTTTEVYDFLRLLYARASTAYSYNTGEEMVRYTDEQIISLIVEKFTGSRTLLLAP
                     LIRGRKGHYKELFEQLRHKGYLQVRVDDEIIEMRPNMKLDRYKTHHIELVVDKMVVEP
                     DNSKRIAQSVATAMNMGKGTIMVLNADDGSWRYFSRNLMCPTTGISYEEPEPYTFSFN
                     SPKGACPHCNGLGYISEADISRIIPDPSVSIKKGGILPLGPYKNSLIFWQIEAIASKY
                     GFSISDPISTIPEEALNVILYGSEEQFKLKHTGLGLTSSYFLSFEGVVSFVQNHSSND
                     ELNGKGDKWANQFIRQVTCPHCCGSRLKNESLWFKIDGKSIADVASMDIVSLSKWLQG
                     LEDRMNSRQRAIAAEILKEIRERVGFLLDVGLGYLSLSRGTVTLSGGESQRIRLATQI
                     GSKLVNVLYILDEPSIGLHQRDNVRLINSLKQLRDSGNSVIVVEHDEETILSADYVVD
                     IGPLAGRHGGRVMATGTPAEVMKTNSLTAQYLRGEREIAVPTKRRKGNGKIVRIRGAR
                     GNNLKNIDVEFPLGTMICVTGVSGSGKSTLVNETLQPALSQQFYNSYKQPLDFDGIDG
                     IEHIDKVIEVDQSPIGRTPRSNPATYTGVFTDIRKLFELTPDAKIRGFKAGRFSFNVK
                     GGRCETCKGAGVRVIEMNFLPDVYIKCPDCYGQRYNRETLEVKYKGKNISEVLDMTIN
                     QAVEFFGSIPQIHQKLKTLQEV"
     gene            complement(2483..3241)
                     /locus_tag="PLX53_03060"
     CDS             complement(2483..3241)
                     /locus_tag="PLX53_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOY72089.1"
                     /translation="MRESLDRELQVNTFWHSQTVMLLKRANRYFPVIEPILQKNGIPD
                     DFKYLAVAESGLMQVVSPSKAAGIWQILEGTGKDYGLEVNSEVDERYHVEKSTEVACR
                     YLNKSYRKYGSWTMAAASYNYGQNGMDKQIDRQQGDSYYNMVLGEETGRYVYRILAIK
                     VIFENPRKYGFFLDSADLYPPFRYREVSVDTAISSISNFARRFDTNYKVIKLFNPWLR
                     ESYLNNKAGKHYRIKIPDINFRVEAYAAGKKDEQ"
     gene            3343..>3551
                     /locus_tag="PLX53_03065"
     CDS             3343..>3551
                     /locus_tag="PLX53_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72090.1"
                     /translation="MMQPFQNAAAGAVFSLFCAAAPSLALAEGEASPEIVVTGAALPE
                     AQSQPNYSVVTIDADRLRSSASGRI"
BASE COUNT          791 a          902 c          894 g          964 t
ORIGIN      
        1 caacctcctg tagggttttc agcttctggt gaatctgggg gatgctgcca aagaactcca
       61 ccgcctggtt gatggtcata tccagcacct cgctgatgtt cttgccctta tacttcacct
      121 caagggtttc gcggttgtaa cgttgcccat agcagtcggg gcatttgatg taaacatcgg
      181 gcagaaagtt catctcaatt accctaacgc cagcaccttt gcacgtttca caacgacctc
      241 ctttcacgtt aaacgaaaac cggcctgcct taaatccacg aatcttggca tcgggtgtca
      301 gctcgaacag cttgcggata tcggtgaata ctccggtgta ggtagccgga tttgatcggg
      361 gtgttctgcc tatgggcgat tggtcaacct caattacctt gtcgatatgc tcaattccat
      421 ctataccatc gaaatcgagt ggttgcttgt aggagttgta aaactgttgg ctcagcgctg
      481 gttgaagggt ttcgttaacc agggttgatt ttccgctgcc cgaaacgccc gtaacgcaaa
      541 tcatggttcc aagcgggaac tccacatcaa tatttttaag gttgttaccc ctggcacccc
      601 ttatgcggac gattttccca tttcccttac ggcgttttgt agggacagca atctcccttt
      661 cgcctctgag gtactgggcg gtaagggagt tggttttcat tacctctgca ggtgttcctg
      721 ttgccatcac cctgcctcca tgacgaccgg ctaatgggcc aatatccacc acgtaatcgg
      781 ccgagaggat tgtttcctca tcatgttcta ccactataac cgagtttccg gaatcgcgca
      841 gctgcttcag ggagttaatc aaccttacgt tatcgcgctg gtgtagccca atacttggct
      901 cgtcgagtat gtataggacg tttaccagct tcgacccaat ttgggtggcc aggcgaatgc
      961 gttggctttc gccacccgaa agggtaaccg ttccacggct caacgacagg tatcctaggc
     1021 caacatcaag caggaagcct acccgttcgc ggatttcctt cagtatttct gctgcaatag
     1081 ctcgttgccg gctgttcatc ctgtcctcca acccctgtag ccacttcgag aggcttacga
     1141 tgtccatgga ggcaacatcg gctatgcttt taccgtctat tttaaaccaa agcgactcgt
     1201 ttttaagtcg ggagccgcag caatgcgggc aggtaacctg acggataaac tggtttgccc
     1261 acttgtcacc ctttccgttt agctcatcgt ttgagctgtg gttctgcaca aagctaacta
     1321 ccccttcaaa acttagaaag tatgaagaag taagccctaa ccccgtatgc ttcagcttga
     1381 attgctcctc ggaaccataa aggatgacgt tgagggcttc ctcggggatg gtagaaattg
     1441 gatcagaaat tgaaaaccca tacttagaag caattgcctc tatttgccag aatatcaacg
     1501 agtttttata gggtccaagg ggaagaattc cgcctttttt aatgcttaca gaagggtcgg
     1561 gtattatgcg ggatatatcg gcttcggaaa tgtaacccag gccgttgcag tgggggcaag
     1621 cgcctttcgg ggagttgaac gagaatgtgt atggttcggg ttcttcgtag gatattccgg
     1681 tagtagggca catcaggttg cggctgaagt atcgccacga accatcatcg gcgttgagca
     1741 ccatgattgt ccctttgccc atgttcatgg ctgtggccac cgattgagca atacgcttgg
     1801 agttgtccgg ttcaaccacc atcttgtcca ccaccagctc tatgtggtgg gttttatagc
     1861 ggtcgagctt catgtttggc cgcatctcga taatctcatc gtcaaccctt acctgcaggt
     1921 agcccttgtg ccgaagctgt tcgaatagct ccttgtagtg gccttttcgt cctcgaatga
     1981 gtggagcaag caaaagggtt cggctgccgg taaatttttc aacaattagg ctgataattt
     2041 gctcgtcggt gtagcgaacc atctcctcgc cggtattgta ggagtaggcg gttgaagcgc
     2101 gagcgtagag caaccgtagg aagtcataaa cctcggtagt cgtgcccacc gtggaacgtg
     2161 ggttgtggct ggtggttttt tgctcgatgg aaattacggg gctaaggccg gaaattttat
     2221 ccacgtcggg acgttcgagc gttcccagaa actgacgtgc ataagccgac atggtttcca
     2281 tgtaccttcg ttgcccctcg gcgtatatgg tatcgaaggc cagcgatgac tttccgctac
     2341 cgctcaaacc ggtaataacc gttagttttc cacgaggtat ggcaacatca atatttttca
     2401 ggttatgcac ccgggcgccc tcaaccacaa cttcttcttc ttcacccttc aaaaattcca
     2461 tttctaactc caaattagtc ccctattgct cgtccttctt ccctgctgcg taagcttcaa
     2521 ccctgaaatt tatatccgga attttaattc tgtagtgttt ccctgccttg ttgttcaggt
     2581 agctttcgcg tagccatggg ttgaaaagtt taattacctt gtagttggta tcaaaccggc
     2641 gggcgaaatt agaaatgctg gagattgccg tatcaacgct aacctctcta tacctaaacg
     2701 gggggtaaag gtcagccgaa tcaaggaaaa aaccatactt acggggattt tcaaaaatta
     2761 ccttgatggc caaaattcta taaacatagc gaccggtttc ctcacccagc accatgttgt
     2821 agtatgaatc gccttgctgt cggtcaattt gtttatccat accattttgg ccgtagttgt
     2881 aggaggcagc agccatggtc cagctgccgt attttcggta gcttttgttc aaataccggc
     2941 aggccacctc ggttgatttt tcaacgtggt agcgctcgtc aacctcactg tttacctcaa
     3001 gaccgtaatc tttgcccgtg ccctcgagaa tttgccaaat tccggcagcc tttgatgggg
     3061 aaacaacctg catgagaccg ctttcggcaa cggcaaggta tttaaaatca tcaggaattc
     3121 catttttttg gagaataggc tcgataaccg ggaagtaccg gttggctctt ttgagcaaca
     3181 taacggtttg ggagtgccaa aaggtattta cctgcagctc cctgtccagg ctttcgcgca
     3241 catcgaagta atcgagtgga acattttcgc cggcaaagga gaggttttgg gggattggga
     3301 tggggagcgt gcagcgccct gcctgggaaa tgttcgaggg gattgatgca gccgtttcaa
     3361 aacgccgccg cgggcgcagt attcagcctg ttttgcgccg ccgcgccgtc tttggcgctg
     3421 gccgagggcg aggcgtcgcc cgaaatcgtc gtcaccggcg ctgctttgcc cgaggcgcag
     3481 agccagccga actacagcgt tgtgacgatc gacgcggatc gcttgcgttc atccgcctcc
     3541 gggcggatcg a
//
LOCUS       DAOOQX010000134         3541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_31528_length_3541_cov_3.274527, whole genome shotgun sequence.
ACCESSION   DAOOQX010000134 DAOOQX010000000
VERSION     DAOOQX010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31528_length_3541_cov_3.274527"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..944)
                     /locus_tag="PLX53_03070"
     CDS             complement(24..944)
                     /locus_tag="PLX53_03070"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOY72091.1"
                     /translation="MKKILLLLLAAIITLTSVGQGTVKKGKASTGPYPRGWVLVEKGG
                     KYGFISAEGDEIIKTQYTRISPFDEYLSGWALVEKDGFLGFISNSGDEVVKPQYTKIY
                     PFDEMQRGWAMVEISGLFGFISSEGREVVKPKYSKIYHFGEMYKNLAMVEKDGLVGFI
                     NLDGSEVLKPQYQKVHPFGEYKKDWAMVERDGFFGFINGEGKEVVAPKYSKVSPFGEY
                     QKNWALVEKDRYLGFIDMDGREVIKPQYSQILPFGQLQKDWAMVERDGLWGFISADGR
                     EVVKPKYEKINLDDVSGIKKTNEVVKDENS"
     gene            1348..2727
                     /locus_tag="PLX53_03075"
     CDS             1348..2727
                     /locus_tag="PLX53_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOY72092.1"
                     /translation="MKRIKILHLAMQVAIAALLLSVSACEKEYPTINSNEQTAVVQDD
                     VQKATATFAENFESGTKTSYTGASVTLATGSWYLNDALLGNSSSDRKNGTKSVRIRNT
                     GKLTMQFDKTTGAGTVQIKHAKYGSDGSSTWELYISTNSGSTWSKVGSTVTTSSTTLT
                     AASFTVNVSGNVRFEVRKISGGSYRINIDDFTITDYGSTPPPSSDPTRDNNMALGNPS
                     GAVTSTAYANNYLMNKTQYSLSYNNSKLTCNWVSWHLSTAWLGSTPRQDDFRADATLP
                     SGWVQVGGSDYSGSGFDRGHMCPSADRTGSVTDNSATFLMTNMVPQAPNNNQITWANL
                     ENYCRTLVNAGNELYIISGPWGQGGTGSNGTKSTISSKNIVVPSYVWKIIVVLPVGSN
                     DVTRITSSTRVIAVWMPNTQTVSSQSWGYYRTTVDYLESKTGYNFLSNVSTTIQSAIE
                     SKVDNGPIN"
     gene            complement(2802..>3541)
                     /gene="serS"
                     /locus_tag="PLX53_03080"
     CDS             complement(2802..>3541)
                     /gene="serS"
                     /locus_tag="PLX53_03080"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOY72093.1"
                     /translation="LINFFLDCNTAAGYQEVEPPHMVNEDSAMGTGQLPDKDSQMYHI
                     GLDGFYMIPTAEVPLTNMFRDVILTSSMLPVKVTAYTPCFRREAGSYGKDVRGLNRLH
                     QFDKVEIVQVQHPDKSYQSLEEMVLHVEGILKKLELPYRIVKLCGGDMSFTSALTYDF
                     EVFSAAQKKWLEVSSVSNFESFQANRMMLRFKDDGDKRTQLAHTLNGSSLALPRIVAA
                     LLENNQSPNGIRIPAAIKNYCGFDLIS"
BASE COUNT         1012 a          916 c          712 g          901 t
ORIGIN      
        1 tgcgatcctg accgaatctt cgctcagcta ttttcgtcct ttacaacctc gttggttttt
       61 ttgatgcccg aaacatcatc aaggttgatt ttctcgtact tcggcttcac cacctcccgg
      121 ccatcggctg aaatgaaacc ccaaagcccg tcccgctcaa ccatagccca atccttttgt
      181 agctgaccaa acggtagtat ctggctatac tgtggcttaa tcacctccct gccatccata
      241 tcaataaaac ctaagtagcg gtctttctca acaagcgccc aattcttttg gtactccccg
      301 aatggagaaa ctttactgta ctttggagca acaacctctt tcccctcccc atttataaag
      361 ccaaaaaatc catcgcgctc caccatggcc caatcctttt tatactcgcc aaatggatgc
      421 actttttggt actggggctt aagcacctcg cttccatcta aatttataaa acctaccaac
      481 ccatccttct ccaccatggc gaggttctta tacatttccc caaaatggta aatcttgctg
      541 tactttggtt tcacgacttc cctgccttcg cttgatatga acccaaacaa cccgctgatt
      601 tcgaccatag cccagccacg ctgcatctca tcaaaaggat aaattttggt gtactggggt
      661 ttaacaacct catcacccga gttggaaata aaccctaaaa agccatcttt ctctaccagc
      721 gcccatcccg acaggtactc gtcgaaggga gatattctgg tgtactgggt tttaataatc
      781 tcatctcctt cggccgatat aaacccgtac ttgcctccct tttcaaccag cacccatcca
      841 cgggggtatg gacctgttga agctttaccc tttttcacgg ttccctgccc aaccgaggta
      901 agggtgataa tggcagcaag caaaaggagt agaatttttt tcatatcaag aggtttatgc
      961 gtaaatatga atctcggttt cccaatactt aacagataca aacctactga aattttttat
     1021 gatacaccaa agaggtaagg gtaattctgc cccacaacct taaaaagtca tgtccaaaag
     1081 ttaatgcggc taataaattt taccgcattc ccgggatgac tcgttaaaaa agtcaataat
     1141 taaacggaga acccgtaatt ccaactaaat agggcattta taagacattg gaataaaggc
     1201 atgtagaaaa ttcaaacggc taaacctaat tttccagcat ttataacata ttgttaacac
     1261 tatccaaata aaataggtgc atttttgcta cttaagcaaa caaaactatt tcataatttt
     1321 attgcaccaa cctaaaccta tctaaccatg aaacgaatta aaattttaca tctcgccatg
     1381 caagttgcaa tagctgcact gctcctgtcg gttagtgcct gcgagaagga gtatccgacc
     1441 attaattcta atgaacaaac ggctgttgtg caggatgacg ttcagaaggc taccgctact
     1501 ttcgccgaaa attttgaaag tggaacaaaa acctcctata caggtgcatc ggttaccctt
     1561 gctacaggaa gctggtacct gaatgacgcc ctgctaggaa attcatccag cgacaggaag
     1621 aacggaacaa aatctgtaag gattaggaac accggcaagc tcaccatgca gtttgataaa
     1681 acaactggag ccggaacggt gcagattaaa catgccaagt acggcagcga tggcagcagc
     1741 acttgggagc tttacatctc aaccaacagc ggtagcacat ggagtaaggt gggctcaacg
     1801 gtaaccacct catctaccac tctaactgct gcttccttta cagttaacgt atcgggaaat
     1861 gtacgtttcg aagtaagaaa aatatccggc ggttcgtacc gaataaacat tgacgatttc
     1921 acaattactg actacggctc tactcctcct ccatcgtccg atcctacccg ggataacaac
     1981 atggctttag gaaacccgtc gggtgcagta accagtaccg cctacgccaa caactacctg
     2041 atgaacaaaa cgcagtactc cctatcatac aacaacagca agctaacctg caactgggtg
     2101 tcgtggcatc tgagcacggc atggctgggt agtacccctc gtcaggatga tttcagggcc
     2161 gatgccaccc ttccctcggg atgggttcag gtgggaggaa gcgactacag cggctccggc
     2221 tttgaccgcg gccacatgtg ccccagcgct gaccgcacag ggtcggttac cgacaactcc
     2281 gccaccttcc ttatgaccaa catggtaccc caagccccaa ataacaacca gattacctgg
     2341 gcaaatttgg aaaactactg ccgtacactg gttaatgcag gtaacgagct ctacatcata
     2401 tccggcccct ggggtcaggg tggaacaggc tctaacggca ccaagtcaac catctcatct
     2461 aaaaatatcg ttgttccctc atacgtttgg aaaattattg ttgtgctccc tgttgggtca
     2521 aatgatgtta cgcgtatcac ctcatcaaca agggttattg ctgtttggat gcccaacacc
     2581 caaaccgtaa gcagccagtc atggggctac taccgcacta cagttgacta ccttgaaagt
     2641 aaaacaggat acaatttcct ttcgaatgta tcaactacaa ttcaaagcgc catagagtca
     2701 aaagttgaca atggcccgat aaactagaga ggagtaattc tcccttttac aaatgaaaaa
     2761 gccctgcaac ccagggcttt tatcatattt ctaaacaaac cttagctaat caaatcaaac
     2821 ccacagtaat tcttaattgc agcggggatt cttattccgt taggcgattg gttattttcc
     2881 agcagagctg caacaattct gggtagagcc aacgagctgc cattgagggt gtgggctagc
     2941 tgggttcttt tatcaccatc atccttaaag cggagcatca tgcgatttgc ctgaaacgat
     3001 tcgaagttgg aaaccgagct cacctcaagc cacttttttt gtgcggctga aaacacctca
     3061 aaatcatagg ttaacgccga agtaaagctc atatcacccc cgcaaagctt aacaatacga
     3121 tatggaagct ccagcttttt aagaattccc tcaacatgca aaaccatttc ctctagcgac
     3181 tggtaagatt tgtcgggatg ctgtacctga acaatttcta ccttgtcgaa ctggtgcagg
     3241 cggttaagcc ccctaacatc ttttccatag gagccggcct cacgtctgaa gcatggagtg
     3301 taggctgtta ccttaaccgg aagcatggaa gaagtcagga taacatccct gaacatattg
     3361 gtaagcggta cctccgcagt aggaatcatg tagaaaccat ccaggccgat gtggtacatc
     3421 tggctatcct tgtcgggtag ctggccagtg cccatggccg aatcctcgtt gaccatgtgg
     3481 ggtggttcaa cttcctggta gcctgcagcg gtgttgcaat caagaaaaaa gttgataagc
     3541 g
//
LOCUS       DAOOQX010000135         3511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_31915_length_3511_cov_3.126736, whole genome shotgun sequence.
ACCESSION   DAOOQX010000135 DAOOQX010000000
VERSION     DAOOQX010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31915_length_3511_cov_3.126736"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PLX53_03085"
     CDS             <1..599
                     /locus_tag="PLX53_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HOY72094.1"
                     /translation="GNVVVSQPIKGTAPRMALPDMDSQMVEKLKSDAKEISENVMIVD
                     LVRNDLSRVAVPGSVKVNELCGIYTFRQVHQMISTVSCIVPEKLNLVEPIKATFPMGS
                     MTGAPKVRAMQLIEEFEKSRRGIFSGAVGYITPNKNFDFNVVIRSLLYCGQRKYLSFS
                     VGGAITAKSDPENEYNECLLKAKAILQTLNARIENVAG"
     gene            586..1905
                     /gene="tilS"
                     /locus_tag="PLX53_03090"
     CDS             586..1905
                     /gene="tilS"
                     /locus_tag="PLX53_03090"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOY72095.1"
                     /translation="MLQDKFINFIRVHRLADENDSILLGVSGGIDSMVMLHLFQKTGF
                     KFAVAHCNFGLRGEESDGDEEFTRTYCREKGVVFHTTKFSTVQYATDKSVSIQVAARE
                     LRYDYFNELCREHGYNKIAIAHNMNDSAETVLLNLIRGAGIRGLTGIRPVHGNIIRPL
                     LFATRPEIENYSKQLGISYREDSSNATLKYKRNFIRHRVIPLLQELNPSVVETIYDSA
                     NHITDAIGLVDRQLDEIRRKVVSFRKDAVLFSVEELKREPAARIFLVEELRHYGFSGN
                     MATQVFDLLESTPGKYVDSPTHTLLRDRKYLILSAKISTSNVCIEVGEDCSRIEKPFP
                     MRFERIVEVKIDSSPQVAILNYSKLRFPLTLRCWQPGDRFIPLGMKGYKKVSDYLVDC
                     KVPLNQKNEVYVLLSNNEIAWVVGYRIDDRYKISTETAEALKISVLL"
     gene            complement(1997..2452)
                     /locus_tag="PLX53_03095"
     CDS             complement(1997..2452)
                     /locus_tag="PLX53_03095"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72096.1"
                     /translation="MKRLTLLLLLVPFLSGAQNNVTEKNGLYLDSNGKPFTGTYQEYF
                     ENGQVKSQMEIVHGLLNGQVKVYFPSGELNEIRSYIKGEKSGKWETWNAQGVKVAEAN
                     FLNGKKNGKWYVWDEKGTLRYDMTYRMGEKSGTWIIYNDKGLETSRKEY"
     gene            complement(2780..>3511)
                     /locus_tag="PLX53_03100"
     CDS             complement(2780..>3511)
                     /locus_tag="PLX53_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72097.1"
                     /translation="FRVVFGTVNAKYLVSYRGGDDDFDTDNGFSGNVQYGLILRGPEE
                     ADKSDASNGFESDNDANGSSNTPLTSAKFSNITILGPYATKTQPNVNPNHKHALRLRR
                     NTRLSLYNSILAGFATGPRIEGPSMTQYNTTGGPEIKNCIMAGMIKDFQVNTDGTTLT
                     QEQYDAMWTAGNNQTFAENADIKLTNAWSWGNVNPMPQSGSPVLTGASFSGLSGFETV
                     AFIGGFGTTNWTSGWCSWDPQNARY"
BASE COUNT          968 a          741 c          819 g          983 t
ORIGIN      
        1 acgggaatgt agtggtaagc cagccaatta aaggcactgc gccaagaatg gctttgccag
       61 acatggattc gcagatggtg gaaaagctaa aatcggacgc caaggagata tccgagaatg
      121 tgatgatagt tgatttggtc cgaaatgacc tgtcgagggt tgcggttccc ggttcagtta
      181 aggttaacga gctctgcgga atctacacct tccggcaggt tcaccagatg atttccaccg
      241 tttcgtgcat cgtgcctgag aagctaaacc tggtagaacc cataaaagcc acattcccta
      301 tgggatctat gaccggtgcc ccaaaggtaa gggcaatgca gctgattgaa gagtttgaaa
      361 aatccaggag gggaattttt tcaggcgcag tgggctacat cactcctaac aaaaattttg
      421 atttcaacgt ggttatccgt agcttgcttt actgcgggca aaggaagtac ctttcctttt
      481 cggtgggagg tgcaattacc gccaagtccg accctgaaaa tgaatacaac gaatgcttgc
      541 taaaagccaa agccatactt caaacgttaa acgccaggat tgaaaatgtt gcaggataaa
      601 tttatcaatt tcattcgggt acaccggttg gccgacgaga acgactccat cctgctgggt
      661 gttagcggtg gcattgattc catggtaatg ctgcatcttt ttcaaaagac tggtttcaaa
      721 tttgccgtgg cccactgcaa ttttgggtta aggggtgagg agtctgatgg cgacgaggag
      781 tttacccgaa cctactgccg tgaaaaagga gtggtgttcc acactactaa atttagtacg
      841 gttcaatacg ctactgataa gagtgtttcc attcaggtgg ctgccaggga gttgaggtat
      901 gactacttca acgagctttg tcgggagcac ggctacaaca aaattgccat tgcccataac
      961 atgaacgaca gcgcagaaac ggttttgctc aacctaatcc gaggggcagg tattagaggg
     1021 ttaacaggta tcaggcctgt tcatggaaat ataattcgac cgttgctctt tgccactcgc
     1081 cctgagattg aaaactactc aaagcagctg ggaattagct accgtgagga ttccagcaac
     1141 gcaaccctaa agtacaaacg aaatttcata aggcatagag tcattccgct gctacaagag
     1201 ttaaacccat cggtggtgga aacaatttac gattcggcca atcatatcac tgatgctata
     1261 ggtttggttg acaggcagct ggacgagata agaaggaaag ttgtttcatt cagaaaggat
     1321 gctgtgctat tttcagttga agaactaaag cgtgaaccgg cagcccgtat atttctggtt
     1381 gaggagctac ggcattacgg gttttcggga aatatggcaa cacaagtctt tgacctgctg
     1441 gagtcaactc caggaaagta tgttgactcg ccgactcata ccctgctaag ggatagaaaa
     1501 tacctgattc tgtcggctaa aatttctaca agtaatgtct gcatagaggt tggcgaagat
     1561 tgcagccgga ttgagaaacc ctttcccatg aggtttgagc gtattgttga ggttaagatt
     1621 gattcaagtc cgcaagtagc catccttaac tactctaagc taaggtttcc gcttacactt
     1681 cgatgctggc aacctggcga taggtttata cctttaggca tgaaaggcta taaaaaggta
     1741 agcgattacc ttgttgactg taaggttcct ttaaaccaaa aaaacgaggt gtatgttctg
     1801 ctttccaata atgaaattgc ctgggtggtt ggctacagga ttgacgacag gtataaaata
     1861 tccactgaaa cagctgaggc cttgaaaatt tcggtgcttt tgtagcaata caacggcaga
     1921 aatgcaaaca caaccacccc cctttaaggc agcggtacct gattacgtga agtttaacct
     1981 tttgaagagt gggagactag tattcttttc gggaagtttc taagccttta tcgttgtaaa
     2041 ttatccatgt tccggatttc tcccccatcc ggtaggtcat atcgtagcgt aaagtcccct
     2101 tttcgtccca cacataccat ttaccatttt tctttccgtt gagaaagttg gcttcagcaa
     2161 cttttacccc ctgagcattc catgtttccc attttccgct tttttcccct tttatgtacg
     2221 accgaatttc attcagctca cccgacggaa aatagacttt tacctgcccg ttgagtaagc
     2281 cgtggacgat ttccatttga gattttacct gcccgttttc aaaatactcc tgatatgtgc
     2341 cagtgaaggg tttaccattg ctgtcaaggt atagcccatt cttttctgta acattatttt
     2401 gggcaccgga cagaaaagga actaaaagta aaagtaacgt aagtcttttc atattatggt
     2461 gtttagcagg ttgatagtta atgtgtaaaa tatttttatg ctataaaaca gcttagtaga
     2521 atactcttaa tttttacttg aaacaggggc tggaggtttc atctgcaaag atttttttgg
     2581 tttcttatta aaaatactaa ggtccatacc gtttaagtat cggtgttaaa gctgatggaa
     2641 catccatatt ttggcttcac caatcccgca agaagtgaac ggttgttcat gtgcagtata
     2701 aacgattaga aatgtagtag tatattcaaa aaaaacttct cccggattaa gggagaagct
     2761 tttaaggttg gttataacat tagtatctag cgttctgtgg atcccagctg caccacccgc
     2821 tggtccagtt tgtagttcca aaaccaccaa tgaaggcaac tgtttcgaag ccagacaacc
     2881 cgctaaagga agcgcctgtg agaacaggag aaccactttg gggcatagga ttaacatttc
     2941 cccagctcca tgcattggta agttttatat ctgcattttc agcaaaggtc tgattgttac
     3001 ctgctgtcca catggcgtca tactgctcct gggtaagggt agtcccatcg gtgttaacct
     3061 gaaaatcctt tatcatgccg gccattatgc agttcttaat ttcaggaccc cctgtagtgt
     3121 tgtattgcgt catgctagga ccctcaatac gaggaccggt ggcaaaacct gccaggattg
     3181 agttatagag gctaaggcgg gtattccgac gcaacctaag agcatgcttg tggttggggt
     3241 taacatttgg ttgggtctta gtggcatagg ggccaagaat agtgatgttg ctgaactttg
     3301 cagaggttaa tggagtattt gatgagccgt ttgcatcgtt atcggattca aacccgttgg
     3361 atgcatcgga cttgtcagct tcctctggac cacgaagtat taaaccatac tgaacatttc
     3421 cgctaaagcc attgtcggtg tcaaaatcgt catcaccacc acggtacgaa accaggtact
     3481 tggcatttac ggttccgaaa accactcgaa a
//
LOCUS       DAOOQX010000136         3501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_32039_length_3501_cov_2.666570, whole genome shotgun sequence.
ACCESSION   DAOOQX010000136 DAOOQX010000000
VERSION     DAOOQX010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32039_length_3501_cov_2.666570"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLX53_03105"
     CDS             complement(<1..372)
                     /locus_tag="PLX53_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72098.1"
                     /translation="MSLLTSTKGLAFAAILLGLGMASCNDDEDEPKPPTGQTVTVSGD
                     ITANTTWSANDTIMLEGFVYVNNGVTLTIEPGALIKGLSDTKAALIVERGGKIMANGT
                     QDRPVVFTSDKPAGQRKPGDWA"
     gene            complement(574..939)
                     /locus_tag="PLX53_03110"
     CDS             complement(574..939)
                     /locus_tag="PLX53_03110"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY72099.1"
                     /translation="MKRILVLLIAALTLNVFGATTDDKTKGESTTSLPVKSITINGKV
                     TDKLTGEALAGVAVKTDGSTTCYSDFDGNFSIQVNPEKLENLSAELISYSTSVVKVDI
                     NKTINPTLKIKIELEPIAE"
     gene            complement(1049..>3501)
                     /locus_tag="PLX53_03115"
     CDS             complement(1049..>3501)
                     /locus_tag="PLX53_03115"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOY72100.1"
                     /translation="RKTSTNLAMISSIKASLMVATGISAQQINNSQDKDASEVIRRVP
                     GASIIDDRFVVVRGLNQRYNSVWINNASTPSSETDQKAFSFDVIPSAMIDNMMVSKSM
                     SPELPADFTGGFIKIFTKNMPESNFMSISYTTGYNTRAHYGDFQLITTGKTDWLGVDG
                     GTRNLPSGFPNSLKGYPDAELADLGKRLNTSWAPKGVSAFPEQKLNFSFGRKIQKGNR
                     LLGNITSISYSHTYSSDELVNKGYQVQLNPGEIPPYSYNYTDSTYSRSVRLGILHNWS
                     LFPGKGTKIEFRNLFNLMGNNTAVIRNGLNGYEGYTIRSYQDKYMSRITYSGQLGGEH
                     KLGSNSDNTLDWVLGFAYSGRNEPDLKQLRTTLQSEPLLPHYNEYYASVGLTPSVSDA
                     GRLFMNLHEYIATAGTNYEAKIKIGSWKPVIKTGLYSEYKNRTFDARILGFAKNVSYT
                     ESVWLPVETIFSLENINTTPSGFILKEMTSRSDSYDATNLLLAGYLTINTNITQKLRF
                     YGGARFEMNNMRLNSYDMYNKPVDVKLDHIDIFPSANLTYNFNEKNLLRLAGGLSVNR
                     PEFREIAPFYFYNFDEEADYVGNVNLKNAYVWNFDFRYELYPNAGEIISIGIFYKHFT
                     SSIESVFRPAGNRLTFTYDNADNAQSAGAEVELRKSLEFIPLLKDFSAVLNASYIYSR
                     VNFPANSIFRDRPMQGQSPYLVNAAVFYSNTKHKLNLSLQYNLIGDRILAVGLPFQNS
                     NQDIPDITEKHSHLVDFTASKGIGSKMEIKAGIKNLLNQKSIKYQAFKQADGGDLNLS
                     SREMQPGITFSLGFSLNF"
BASE COUNT          831 a          733 c          821 g         1116 t
ORIGIN      
        1 tgcccagtcg ccaggttttc tctgaccggc cggtttatcg ctagtaaata caacaggtct
       61 atcttgtgtt ccattggcca taattttacc gccacgctca actatcagcg ctgccttggt
      121 gtcgcttaaa cccttaatta gcgctcccgg ctcaatggta agcgtaaccc cattgttcac
      181 gtaaacaaac ccttccagca tgatagtatc gtttgccgac caggttgtgt tggctgtgat
      241 atcgccagaa accgttaccg tttgtcctgt gggaggcttg ggttcgtctt catcatcatt
      301 acatgatgcc atgcccagac caagtaggat tgccgcaaag gctaaaccct ttgtgcttgt
      361 taagagactc atttttttca ttttgattga ttttagaatt atctggcaca aaagtagggc
      421 tgagcagcta aagcgcagct aagcaaaaat taccctttca ttagctgttt gttaggtatc
      481 tgttaacagg gaagtggtag tgtttctaga atgaaaaagc cacacctggg tgaaggtgtg
      541 gcttagtggt gaacttagct acgatgagtt actctactca gcaatgggtt ccagctcaat
      601 ctttatcttc agtgtagggt tgatagtttt atttatgtca acctttacaa ctgaagtaga
      661 ataggagata agttcggcac taaggttttc gagtttctcc ggattgactt gtatgctgaa
      721 gttcccatca aaatcggagt agcaagttgt gcttccatca gttttgacgg caacccctgc
      781 cagtgcttcc ccggtaagtt tgtcggtaac tttcccgtta atggtgattg acttaacggg
      841 caagctggtt gtgctttcac cttttgtttt atcatcagtg gttgctccaa aaacattcaa
      901 ggttaaagca gctattaaca gcactaaaat tcttttcatg ggtatttttt ttacttttgt
      961 ttctgaagca aaggtagtat ggtggtattt ccttaccatt atatcaatat gattaaaatg
     1021 ttaagttgat gttaaacagg ttttgatgct aaaaattcag tgaaaagccc aggctaaatg
     1081 ttatcccggg ctgcatttcc ctactgctca agtttaaatc acctccatca gcctgtttaa
     1141 acgcttggta cttgatgctc ttttgattca agagattttt gatacctgct tttatttcca
     1201 ttttgctgcc aattcccttg ctggcggtga agtcaaccag gtggctatgt ttttcggtga
     1261 tatcggggat gtcctggttg gagttttgga atggtagccc cacagctaga atcctatcac
     1321 ctataagatt atactgcaac gatagattta gcttatgctt ggtgttgctg taaaacactg
     1381 ctgcattaac caggtagggc gattgtccct gcatgggtct atcacgaaat atagagtttg
     1441 caggaaagtt tacccggctg tatatgtatg aggcattaag cacagcgctg aaatccttta
     1501 gcaggggaat aaactccagt gattttcgta gctcaacttc tgctcctgcg ctttgggcat
     1561 tatctgcatt atcgtaggta aatgttaagc ggtttccggc gggtctgaac accgattcaa
     1621 ttgaggatgt aaagtgctta tagaagattc ctatagaaat aatttcaccg gcattcgggt
     1681 atagctcgta gcggaaatcg aagttccaca catatgcgtt ttttagattt acatttccca
     1741 cgtaatcggc ttcctcgtca aagttgtaga agtaaaatgg tgcaatttcc ctaaattctg
     1801 ggcgattaac ggatagcccg ccggctagac gcagcaaatt tttctcattg aagttgtagg
     1861 taaggttcgc cgatgggaaa atatctatgt ggtcgagttt aacgtcaacg ggcttgttgt
     1921 acatgtcgta gctgttgagg cgcatgttgt tcatttcgaa tctcgctcct ccgtaaaatc
     1981 tcagcttttg ggttatattc gtgttgatgg tcaggtatcc tgcaaggagc aggttggtgg
     2041 cgtcgtatga gtctgatctg gaagtcatct cctttagtat aaaaccgctt ggggtagtgt
     2101 tgatgttttc caggctaaaa attgtttcaa ccggaagcca gaccgattcg gtgtaggaga
     2161 catttttggc gaaacctagt attctggcgt caaatgttct atttttatat tcagagtaaa
     2221 gtccggtttt aataacaggt ttccagctcc caatttttat tttagcttcg tagtttgtcc
     2281 ctgcagtggc tatgtattcg tgaaggttca tgaatagcct gccggcgtcg ctcactgaag
     2341 gtgttaaccc aactgaggca tagtattcgt tgtagtgggg cagtagtggc tcagactgta
     2401 gggtggttcg aagttgtttt aagtcgggtt cgttacgtcc cgagtaggca aatcctaaaa
     2461 cccagtcaag ggtattatcc gagttgctgc ccagcttatg ctctccaccc agctgtccgg
     2521 agtaggttat cctgctcatg tatttatcct ggtatgaccg gatggtataa ccttcatagc
     2581 catttagccc attccgaata acggcagtat tgttacccat caggttgaag agatttctaa
     2641 actcgatttt tgttcctttt ccagggaaca gcgaccagtt atgtagaatc cccagcctga
     2701 ccgagcggga gtatgtgctg tcggtatagt tatagctgta tggaggaatt tcgcctgggt
     2761 taagctgcac ctggtaacct ttgtttacca gctcatcgct actgtaggta tggctgtagc
     2821 ttatggatgt tatgttgccc agcaaacggt tacccttttg aatttttctg ccaaagctga
     2881 agttgagctt ttgctcagga aaagcgctta caccttttgg tgcccagctg gtgtttagcc
     2941 tttttcccaa atcggccagc tcagcatcag ggtaaccttt tagggaattt ggaaatcctg
     3001 agggtaggtt tcgggttccg ccatccaccc ctaaccaatc tgttttcccg gtggttatga
     3061 gctgaaaatc gccatagtgt gcccgggtat tgtaacccgt tgtgtagctt atgctcatga
     3121 agttgctttc gggcatattt ttggtaaaaa ttttaatgaa tccaccggta aaatcggccg
     3181 gtagctcagg cgacatgctc ttggatacca tcatgttatc aatcatggcg ctgggaataa
     3241 catcaaacga gaaggctttt tgatcggtct cgctcgaagg cgttgatgcg ttgtttatcc
     3301 agacgctgtt gtatcgctgg ttcagccccc taactacaac aaatctatca tcaattattg
     3361 aagctcccgg gacacgacgg ataacctcgg aggcatcctt gtcctgtgaa ttgtttattt
     3421 gctgcgccga tatgcctgtt gccaccatta ggctcgcctt tatagagcta atcatggcta
     3481 gattggtgct ggtttttcgg g
//
LOCUS       DAOOQX010000137         3501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_32040_length_3501_cov_2.464887, whole genome shotgun sequence.
ACCESSION   DAOOQX010000137 DAOOQX010000000
VERSION     DAOOQX010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32040_length_3501_cov_2.464887"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..686)
                     /locus_tag="PLX53_03120"
     CDS             complement(210..686)
                     /locus_tag="PLX53_03120"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOY72101.1"
                     /translation="MDKPRSFEIKSQSKGIQLPDDVIPFDLKHVSDLFVSGRQQRYIE
                     IQYFRGSKPENFYARVLFRKEAQGAPDIVHGGAIAAVFDETMGAICLVLGQPAVTATL
                     TVDYRIPVPVEKKFLVEARLEKTERRKFFITARMLDEQQELFAESKAIYVKTKNDG"
     gene            complement(706..1440)
                     /locus_tag="PLX53_03125"
     CDS             complement(706..1440)
                     /locus_tag="PLX53_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HOY72102.1"
                     /translation="METHSTTSNNIKVEIWSDVVCPFCYIGKHRFESALEKFQHSNIV
                     EVEWKSFQLDPYAVTNSNLSVMDNLAREKGLSAAQVQESVGYIADMAKGVGLDFHFEK
                     AIVANSFKAHRILHLAKTHGKQNELEEKLFAAYFTQGMNIDDDDVLLNLATSVGLSGD
                     EVRKVLGSDAYSDEVKHDIAHARQLYVRGVPYFLFDGKYAVSGAQEVDFFLQTLNKAY
                     ADHKGQEVIEIPGNNPGVCGPDGCSI"
     gene            complement(1463..2428)
                     /locus_tag="PLX53_03130"
     CDS             complement(1463..2428)
                     /locus_tag="PLX53_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOY72103.1"
                     /translation="MKIAVAFRTDPDQRKYLVDELGGETELIFLNDLAEDKKMEVLSE
                     VEIILSWNPEKEGITNSKNLGSNLKFIQLMSAGYDHVNPDDFPSNVKIAANQGAYAEP
                     MAEHALAMILALSKRLPLYHQHLVKGEFNQLKSPTKSLRGSVLGIIGFGSIGKATARL
                     AKPFGVKVMALNTSGKTDEEVEYIGTLNDLEYVLRNADSLLISCALNEQTEGLINRQA
                     LELMKPDAVLVNVARGPIVVEKDLYEHLKGHPNFYAGIDAWWIEPFKYGRFELHYPFF
                     ELPNIIGSPHNSAMVENSMIIGTEKAVANLRRFINNEAVRGLINR"
     gene            2717..3085
                     /locus_tag="PLX53_03135"
     CDS             2717..3085
                     /locus_tag="PLX53_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72104.1"
                     /translation="MEIEKIVETIISNAKGLTNDELTKCRSEYDPIYLNDRITHHRKI
                     SNRQNITLFLLVIFAILAFVVVVFGAKVESGTMSWIRGVVTGYFVAIIFLMPKTFKNH
                     SSIASTLSLIKTLREEQKKM"
     gene            3095..>3501
                     /gene="umuD"
                     /locus_tag="PLX53_03140"
     CDS             3095..>3501
                     /gene="umuD"
                     /locus_tag="PLX53_03140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOY72105.1"
                     /translation="MKLVKSTQTIDFYTPDLNAELTLPYADGIRAGFPSPAQDYLDLA
                     FDLNRELVKNPSSTFYGRVRGNSMVDAGINDGDILVIDKSIPPADGRRAVCFIDGEFT
                     LKTLKVEKDILWLLPANPKYKPIKVTPESNFTV"
BASE COUNT          962 a          851 c          727 g          961 t
ORIGIN      
        1 aagaaagcaa gggctacatc gaacggtgta gccctttgta ttaaaattaa accatggtat
       61 tcgggaagca gttaaggcag gcattcataa ttttgaagtg taaactttta aaaagatgaa
      121 aaacctaagc tttacacact ttttgggata ctaccgatta tagcggccta tcttaaaccg
      181 acggaaatgc aacagaaaat tttgacgggt catccgtcgt tttttgtttt cacgtagata
      241 gcctttgatt cggcgaaaag ttcttgctgc tcatcgagca tcctggctgt aataaagaac
      301 ttccgccttt cggtcttttc aagccgtgcc tcaactaaaa atttcttttc aacgggtacg
      361 ggtattcggt aatctaccgt caaggttgcg gtaacagctg gctggcccag caccaggcat
      421 atggctccca tggtttcatc gaacactgcg gctatggcgc caccatgaac aatgtcgggc
      481 gcaccctgag cttccttgcg gaagagtacc ctggcgtaga agttttcggg tttgctaccc
      541 cgaaagtact ggatttcgat gtagcgctgc tgccttcctg aaacaaacaa atccgaaacg
      601 tgtttcaaat caaaaggaat cacgtcatcc ggcagctgga ttccctttga ctggcttttg
      661 atttcaaaac ttctaggttt gtccatggtt cactgcctaa tttatttaaa tactgcaccc
      721 atcaggacca catacgccgg ggttgttgcc tggaatttca atcacctcct gacccttatg
      781 gtcggcataa gccttgttga gcgtttgaag aaaaaaatcc acttcctgag caccagaaac
      841 ggcatacttc ccatcaaaca aaaagtaggg cacgccccta acgtaaagct ggcgtgcatg
      901 ggcaatatcg tgcttcacct catcggagta tgcatcgcta cccagcacct tgcgcacctc
      961 atctccactt agccccaccg atgtggccag gttcagcaaa acatcgtcgt catcaatatt
     1021 cataccttgg gtgaagtagg ctgcaaacag cttttcctcc agctcgtttt gctttccgtg
     1081 ggttttggcc agatgtagaa ttctgtgcgc cttaaaggag ttagcaacaa tcgctttttc
     1141 aaaatgaaag tcgagcccaa cgcccttcgc catatcggcg atgtagccaa ccgactcctg
     1201 aacctgtgct gccgacagac ccttctcccg ggccaggttg tccataacgc taaggttaga
     1261 gttagtaacc gcatacgggt cgagctggaa gctcttccat tcaacctcta cgatgttaga
     1321 atgctgaaac ttctcaaggg cgctttcaaa cctatgcttg ccaatgtagc agaaggggca
     1381 aaccacatcg ctccaaattt caaccttgat gttattacta gtagtgctat gtgtttccat
     1441 ttttttattt atggttaata ctctacctgt tgataagtcc tctaacagcc tcgttgttaa
     1501 taaacctccg gaggttggcc acggcttttt cggttccaat aatcatggaa ttttccacca
     1561 tggccgagtt atggggtgaa ccaataatat ttggaagttc aaaaaacgga tagtgcagct
     1621 caaacctacc atacttaaag ggctcaatcc accaggcgtc gatacctgcg taaaaatttg
     1681 gatgtccctt caggtgctcg tataggtcct tctcaacaac tattgggccg cgggcaacat
     1741 ttacgagcac ggcatcgggt ttcatcagct caagtgcctg acggttaatc aacccttcgg
     1801 tttgctcgtt aagtgcgcat gaaattagca ggctatcggc attcctgagc acgtattcca
     1861 aatcattcag cgttccaatg tactccacct cctcgtcggt ttttcccgag gtgtttaagg
     1921 ccattacttt tacaccaaat ggtttagcca accttgcggt agccttgcca attgagccaa
     1981 aaccgataat ccccagcacc gagcccctaa gcgatttagt agggcttttt agctggttga
     2041 actcaccctt aaccaggtgc tgatggtaca acggcaagcg cttgcttaat gccagtatca
     2101 tggccaatgc atgctcagcc attggctcgg cataggcacc ctggttggca gcaattttaa
     2161 cgttggaggg aaagtcgtca gggtttacat ggtcgtagcc cgccgacatc agctgaataa
     2221 atttcaggtt gctacctaaa ttcttactat tagtgattcc ctctttttca ggattccacg
     2281 ataaaataat ttcgacttcc gaaagaactt ccattttttt atcctccgct aagtcattca
     2341 gaaaaattag ctccgtttcc ccaccgagct catcaaccag gtacttcctt tggtcgggat
     2401 cagttctaaa ggcaactgca attttcatcc ttacaaaata tttactatac taacaattaa
     2461 tgctttacaa acttggcctt gaaaatcaca tttatacatt acatacgctc ataataacac
     2521 ctgtaaacag cattactact taggttagaa cgccttcaat ataaacgtaa agaaattcct
     2581 tgcaggaagc ttccatggag ttccatcctt tctaccacca atgacttgta aacattacag
     2641 gtttgttatt gttcgccaga aataattcgg cataccaaaa ataggagtaa atttgttaat
     2701 caaaactaaa gctgctatgg aaatagaaaa gattgtggag accataattt cgaatgcgaa
     2761 aggtctaaca aatgatgagc taactaaatg tagatcggaa tatgacccaa tctacctgaa
     2821 tgacaggata acccaccatc gcaaaatttc gaaccggcaa aatatcaccc ttttcctgct
     2881 ggtaattttt gccatacttg cctttgtggt tgttgttttt ggggcaaaag tagaatcggg
     2941 aacaatgagc tggattaggg gtgttgtaac cgggtacttc gtggccatta tattcctgat
     3001 gcccaaaact ttcaaaaacc actcctcaat cgccagcacg ctaagcctta tcaaaacgct
     3061 ccgtgaggaa caaaagaaga tgtagcataa acccatgaag ctggtaaaat caacacaaac
     3121 cattgatttt tacacacccg acttaaacgc cgagctaacg ctaccgtatg cagatggcat
     3181 tagggcagga ttcccctcgc ccgcacagga ttatctcgac ttagcctttg acctgaatcg
     3241 ggagctggtt aaaaacccaa gctccacctt ttacggcagg gtgcgtggaa actcgatggt
     3301 tgatgcagga atcaacgatg gagacattct ggttattgat aaatctattc cgccagccga
     3361 tggccgaagg gcggtttgct tcattgatgg tgagtttacc ctcaaaaccc ttaaggttga
     3421 aaaggatata ctttggttac tgccggctaa ccctaagtac aaacccatta aggttacgcc
     3481 cgagagcaac ttcacggttt g
//
LOCUS       DAOOQX010000138         3488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_32185_length_3488_cov_2.547335, whole genome shotgun sequence.
ACCESSION   DAOOQX010000138 DAOOQX010000000
VERSION     DAOOQX010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32185_length_3488_cov_2.547335"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..805
                     /locus_tag="PLX53_03145"
     CDS             74..805
                     /locus_tag="PLX53_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY72106.1"
                     /translation="MSSTNLITLNEIKKFYQVGSVTVKALKGINLTIDQNEYVAIMGP
                     SGSGKSTLMNILGCLDTPTDGTYILNGTDVSRLSDDELADIRNKEIGFVFQTFNLLPR
                     YTSLDNVILPLIYAGVPKHERVEKAKQVLYNVGLGDRMEHKPNELSGGQRQRVAVARA
                     LVNNPSIILADEPTGNLDSKTSIDIMNLFEDIYSQGNTIIIVTHEEDIARHARRIVRI
                     LDGMIESDEVNPHPTLRQKVVLTEQ"
     gene            complement(942..3029)
                     /locus_tag="PLX53_03150"
     CDS             complement(942..3029)
                     /locus_tag="PLX53_03150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOY72107.1"
                     /translation="MQTKKILMALALPALVLASCKTQQPNNEAIVGPVKPVVTDSTLT
                     PEILWSFGRIGEFAVSPDGQKVAYTTAWYSIDQNRGNSEIYIMNTDGTGKRQLTHTNG
                     SEFNLQWVDSLNLAYLATESGKSQIWQMNVENQKTSMVEGFENDISGFSISPTGSKIL
                     LTSDVKVGASIADIYPDLPKSSGQIYTTLMYRHWDTWEDSLFNHVMVANFDGKSLSKI
                     IDIMANEPYDTPLKPFDGMEDIAWSPDGNQIAYTCKKLTGKEAAFSTNSDIYLYDLRN
                     GKTQNLTEGLPGYDKQPVFSPDGEKIAWLSMERAGFEADKDRLLVMDLQTKKTSYLTR
                     DFDYSVSNVIWNASSDMVYFIAGVEATYQIYRLALQDAKPVAITHGWHDYHAFALAGD
                     RLIGSKASIVKPVEIYSINPQNGEEVELSFTNQELLSQLKMPTVRQRWIKSTDGKLVH
                     TWIILPPDFDSTKSYPALLYCEGGPQSAVSQFWSYRWNFSIMASGGYVVVAPSRRGTL
                     TFGQKWTDEISKNHGGQDMKDLLSAIDEVKKEPWVDENRLGAVGASYGGYSVFWLAGN
                     HNKRFKAFIAHCGVFHSEMEYLTTEEMFFDTWEMGGTPWEKNNPVAMNSYAQSPHNFI
                     TSWDTPILIISGGNDFRIPYTQGMAAFNAAQILGVPSKFIFFPDENHWVLKPQNGILW
                     QREFRSWLDRWLK"
     gene            complement(3094..>3488)
                     /locus_tag="PLX53_03155"
     CDS             complement(3094..>3488)
                     /locus_tag="PLX53_03155"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOY72108.1"
                     /translation="IESKPNDMFSVTFAGANRPIFCYSKAENRIQTLKGNRKSIGGLL
                     PDVDLEFTNWRVPMHKGDMIIMYTDGLTDQNNSSKRKFTTARFQTYLLANIEKPMEEI
                     SSILLQEFNEFTGSEPQRDDITILGIRL"
BASE COUNT          871 a          795 c          810 g         1012 t
ORIGIN      
        1 atactaaatt ttaaactttg caccctaaat atttattata catttgtaga acaacataac
       61 ggaacaaaat tcaatgagca gcaccaactt aatcacgctg aatgaaataa aaaaattcta
      121 ccaggttggt agtgttacgg tgaaggcgct aaagggcata aatttaacaa ttgaccaaaa
      181 cgagtatgtg gccattatgg ggccttcggg gtctggaaaa tcaaccctga tgaacatcct
      241 cgggtgtctt gataccccaa ccgatgggac ctacatacta aatggaaccg acgtaagccg
      301 ccttagcgac gacgagcttg ccgatattcg caacaaggag atcggttttg tttttcagac
      361 cttcaacctg ttgccccgct acacctctct cgataatgtt attctacccc ttatttacgc
      421 tggagtgcca aagcacgaac gtgttgagaa ggctaagcag gtgctttaca acgttgggtt
      481 gggggataga atggagcata agcccaacga gttatctggt gggcagcgtc agcgggtggc
      541 agttgcccgg gcgcttgtta acaacccgtc catcattctg gccgatgaac ctaccggcaa
      601 cctcgactcc aaaacctcca tagacatcat gaacctcttt gaggatatct actcgcaggg
      661 taataccata attattgtta cacatgagga ggacattgcc cgtcatgccc gccggattgt
      721 gagaattctt gacggaatga ttgagtcaga tgaggtcaac ccccatccaa ccctgaggca
      781 aaaggttgtc cttacagagc agtagcacaa cattcaacac tccatgttat agctaaattc
      841 cttggacagt cctttaatgc tgaactcctt tcatcaaccg atgaaaggag ttcagcattc
      901 ttgagcctta aaggatcgag tttgacttca acgtaacttc cctacttaag ccacctgtca
      961 agccagcttc taaattccct ctgccagagt attccatttt gaggttttag cacccagtgg
     1021 ttctcatcgg ggaaaaatat gaacttgctt ggaacgccaa gtatttgcgc agcgttgaat
     1081 gcagccattc cttgtgtgta gggaattctg aagtcgttac cgccgctgat gattaaaatt
     1141 ggcgtatccc agcttgttat aaagttatgg gggctttgtg cgtagctatt catggctacg
     1201 ggattgttct tttcccatgg tgttccgccc atttcccatg tgtcaaagaa catctcctcg
     1261 gtggtgaggt actccatttc ggaatgaaaa acaccgcagt gggcaataaa cgctttgaag
     1321 cgcttgttgt ggttgcctgc cagccagaaa acagagtatc caccatagct ggcccctact
     1381 gcacccaggc ggttttcgtc aacccaaggc tctttcttta cctcgtcaat tgccgataaa
     1441 aggtctttca tatcctgacc accgtgattc ttgctgattt catcggtcca tttctgtcca
     1501 aaggtaagcg tgccccttcg gcttggtgcc acaaccacgt atccacccga ggccattatc
     1561 gaaaagttcc agcggtagct ccagaattgg cttactgcgc tttgtggtcc gccttcgcag
     1621 tagaggagtg ctggataact ttttgtgctg tcgaaatcgg gaggaagaat tatccaagtg
     1681 tgcacgagct ttccatcggt tgactttatc catctttgcc taacggtggg catttttagc
     1741 tgggaaagca gctcctggtt ggtaaaggag agctccacct cttctccatt ttgagggtta
     1801 atggagtata tttcgacagg ttttacgatg gaagccttgc tccctatcaa cctatcaccg
     1861 gcaagggcaa aagcatgata gtcgtgccac ccatgggtaa ttgctacagg tttagcgtcc
     1921 tgcaaggcta acctgtaaat ctgataggtt gcctcaactc ccgctataaa gtaaaccata
     1981 tctgagctgg cattccagat tacgtttgaa acagagtagt caaaatcacg ggttaggtag
     2041 gaagtttttt ttgtttgaag gtccattaca agaagtcgat ccttatcggc ctcaaaaccg
     2101 gctcgttcca tggacaacca tgcgattttt tccccatcgg gcgaaaatac gggttgctta
     2161 tcgtaacccg gcaatccttc ggttaagttt tgggtctttc catttcttag atcgtagagg
     2221 tagatgtctg agttagtgct gaatgcggcc tcttttcctg taagtttttt acatgtgtag
     2281 gcaatttgat taccatcggg gctccaggca atatcctcca taccatcaaa tggttttaga
     2341 ggggtatcat agggctcatt cgccataatg tcgatgattt ttgataagga ttttccatcg
     2401 aagttggcca ccatgacatg gttaaacagg ctgtcctccc aggtgtccca gtggcgatac
     2461 attagcgtgg tgtagatttg cccgcttgat ttgggcaggt cggggtaaat atcggcaatt
     2521 gaagctccca cctttacatc acttgtaagg agaatttttg aaccagtagg ggagatgctg
     2581 aatccgctta tgtcgttttc aaatccttca accatggagg ttttctggtt ttcaacattc
     2641 atctgccaga tttgcgattt accactttcg gttgcgaggt aggccaggtt aaggctatcc
     2701 acccattgca ggttaaactc agaaccgttt gtatgggtaa gctggcgctt tccggtgccg
     2761 tcggtgttca tgatgtagat ctccgagtta cccctatttt gatcaataga gtaccaggcg
     2821 gttgtgtatg caactttctg tccatcgggc gacacggcaa attcgcctat tcggccaaac
     2881 gaccaaagaa tctctggagt aagggtgcta tcggttacta caggtttaac aggcccaact
     2941 atagcttcat tatttggttg ctgggttttg catgaggcca gtacgagcgc tggcagcgct
     3001 aaagccatta gaatcttttt agtttgcatt gcttagctat tttggtatag tttactaggt
     3061 agaattagct ttctcacaag gttatacacc gatttaaagt cgaataccca gaatggttat
     3121 atcgtcgcgc tggggttcgc tacctgtaaa ttcattaaac tcctgtagca agatggatga
     3181 gatttcttcc atgggtttct caatattggc gagaaggtag gtttgaaacc gtgccgtggt
     3241 aaacttgcgt tttgaggagt tattctgatc ggttagcccg tcggtgtaca tgataatcat
     3301 gtctcctttg tgcatgggaa ctctccagtt tgtaaattcc agatccacat cagggagcag
     3361 gccgcctatg gactttctat tacccttaag ggtttgtatt ctgttttcag ccttgctata
     3421 gcagaaaatt ggcctgttag cgccagcaaa agtaacggaa aacatatcgt ttggcttgct
     3481 ttcaatta
//
LOCUS       DAOOQX010000139         3479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_32296_length_3479_cov_3.057535, whole genome shotgun sequence.
ACCESSION   DAOOQX010000139 DAOOQX010000000
VERSION     DAOOQX010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32296_length_3479_cov_3.057535"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..1229)
                     /locus_tag="PLX53_03160"
     CDS             complement(252..1229)
                     /locus_tag="PLX53_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72109.1"
                     /translation="MKRYLATLCLATTCTIVLAQSRSELMSREKIQKNKIASVSQWTH
                     KFNKDKVDPKGYVTVVTNYDKNGNEVEIINYKQNGSVSSRHTYKYDNQNRKIEYLQYQ
                     DYRQKGIELAFRQVFTYDKNGNKASELGFDGRTTYRIAYSYFPNGKQKEIIRYNAANQ
                     VEEKWIYEHNGNNTKISIFKPVGTLSKITERKYDSAGNLLDEKNTSGDGKELARTVFT
                     YNANNLPISKSEYYAGELRASYSYVYDGQNQLVETYLIKPNNTRLLYSSYKYDGQGNI
                     VEEKWYEDNATDYSHRNYKLDSKGIVDEVDTYYSDYNFRVVYRYEYKFY"
     gene            complement(1458..2045)
                     /locus_tag="PLX53_03165"
     CDS             complement(1458..2045)
                     /locus_tag="PLX53_03165"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOY72110.1"
                     /translation="MDNIIYFIKSTLASEIASSGFPLIYKGEMNHQIMRSFAFMANRK
                     ISDMDVPTSIRKRVFHIMIECLQNITKHSDDYDEKERQIGNGLFVVGREKDSFFIVTG
                     NLIRNDKMAPLEERLISLNNASPTELKSLFLQQMIGGELTEKGGAGLGLIDIARKSKR
                     KLYYHFVPYENNRHFFILAVTIPIEIDEDDNDENL"
     gene            complement(2176..3165)
                     /locus_tag="PLX53_03170"
     CDS             complement(2176..3165)
                     /locus_tag="PLX53_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HOY72111.1"
                     /translation="MENTGLYPLRFKPIFKPRIWGGNKLRQRLNKNIPKNISNCGESW
                     EVSAVGDNISVIANGFLEGNNLEELVEIYMGDLVGNSVYEKFGIELPLLVKFIDASDN
                     LSIQVHPNDELARERHHAYGKTEMWYVIDADPGAKIITGFNRDVTREEYLESLSTGKL
                     TDLLNVETALAGDVYYIPAGRVHAIGRGVLLLEIQQTSDITYRIYDWDRLDETGKPRE
                     LHTSLALDAIDFSKVSNPKRNITESLNTPIELASCPYFTVNKLTLTQPLKRDYFMIDS
                     FVLYICLAGDVLVNYPNGKEKLATGETMLIPAEIKNISLQPVSTCTIIEVFVG"
     gene            3256..>3479
                     /locus_tag="PLX53_03175"
     CDS             3256..>3479
                     /locus_tag="PLX53_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOY72112.1"
                     /translation="MLIVGIAGGTGSGKTTVVNRIIDLLPKGEVVVLPQDAYYRDSSH
                     LPLEQRQEINFDHPDSIEFELLVEHVKLLK"
BASE COUNT          886 a          687 c          786 g         1120 t
ORIGIN      
        1 gtgtaggaca gcatgaataa acgcgtataa acaaccgggt gtttatggtt aaaaaagtat
       61 tagtaatcag aaacctaaaa atttacacgg aggtatggtt acactatagt agcaatgtgg
      121 caggttgcag gctttaagga tctacacttt agtgtagcct tgacggtggg ttgttctctc
      181 ctccgtaatg aatttttttc tctgtgccag ggtggcggat ggttatcgga atgccgaatc
      241 agtgagctag gtcagtagaa tttgtactcg taccggtaca caaccctaaa gttatagtcg
      301 gagtagtagg tgtcaacttc gtcaactatt cccttgctat ccagcttgta gttacggtgt
      361 gagtaatcgg tagcgttatc ctcataccat ttttcctcca caatatttcc ttgcccatca
      421 tacttatatg agctgtagag tagcctggta ttgtttggtt taatcaggta ggtttcaacc
      481 agttgatttt gtccatcgta aacatagctg tagcttgccc taagctcgcc ggcgtagtac
      541 tccgacttgg aaatgggaag gttgttggcg ttgtaggtaa atacggtacg ggcgagttcc
      601 ttcccatcgc ccgaggtgtt tttctcgtcg agtagatttc cggcggagtc gtactttctt
      661 tcggttattt tgctgagggt gccaacgggt ttaaatattg aaattttggt gttgttaccg
      721 ttatgctcgt atatccattt ttcttctacc tggttggcag cgttgtagcg tataatttct
      781 ttttgcttgc cgttgggaaa atagctgtag gcaatacggt aagtggttct tccatcaaaa
      841 ccaagttctg aggctttatt cccattctta tcgtaggtaa atacctgtct gaatgccagc
      901 tcaattccct tttgacggta gtcttggtat tggaggtatt ctattttacg gttttggttg
      961 tcgtacttgt aggtgtggcg cgacgaaacg gaaccgtttt gcttgtagtt gattatttca
     1021 acttcattcc cattcttgtc gtagttggtt actacggtta cgtagccttt ggggtcaacc
     1081 ttgtccttgt taaatttatg agtccactgt gaaacggagg caattttgtt tttctgaatt
     1141 ttctcacgac tcatgagctc actccgtgac tgggcgagta cgatagtgca ggttgttgcc
     1201 agacagaggg ttgctaaata tcttttcatt atgaacatta ttgagtcaaa aggactaaaa
     1261 ttttgggaaa ggataccagc ttaaaaagaa gtcagttaag gtaagtaagt aattttccac
     1321 aaagatattc aaaaatttat caattttcat accgaagttt tatactgatg gttatcccgt
     1381 gcaggttgag caagaatttt gatgtcgtaa tcggcctacc ggattacgct tatgataagc
     1441 tactgtaaag ggttggttta taggttttcg tcgttgtcgt cctcatcaat ttcaatgggg
     1501 atggttacgg ccaggataaa gaagtggcgg ttattctcat atgggacaaa gtggtagtat
     1561 agctttcgct ttgatttgcg ggcaatgtca attagaccta atccagcgcc ccctttttcg
     1621 gtaagctcac caccaatcat ttgttgaaga aataaacttt taagctcggt gggggaagcg
     1681 ttgtttaggc tgataaggcg ttcctccagg ggtgccattt tatcgttccg aattaggttc
     1741 cccgttacaa tgaaaaaaga atccttttcc cttcctacca caaacaaccc gtttcctatt
     1801 tgcctttcct tttcgtcata gtcgtcggag tgcttggtga tgttttgaag acattctatc
     1861 atgatgtgga aaacccgctt acgtattgac gtggggacat ccatatcgga gattttacgg
     1921 ttggccataa aggcaaacga acgcataatc tggtggttca tctcaccctt atatataagg
     1981 ggaaaaccac tagaggcaat ttcgcttgcc agggtagatt ttatgaaata aatgatgtta
     2041 tccattcagg aaaatgaagg ttgaagatat gaaatttctt catttcaatt catctcttgg
     2101 taggtaaata taggtaaata atttatgaat actataaatt attggataaa acgctcattt
     2161 tatccaacga aaaatttatc caacgaatac ttcgataatt gtacatgtag aaacaggttg
     2221 taggctgata ttttttattt ctgcaggaat aagcatggtt tcgcctgttg ctaatttttc
     2281 ttttccattg ggataattta ccaggacatc gcctgcgagg cagatgtaca gcacaaagga
     2341 gtctatcatg aagtaatccc ttttaagggg ttgggtaagg gttagcttgt ttacggtaaa
     2401 gtaggggcag ctggcaagct ctatgggagt gttaaggctt tcagtaatat ttcttttggg
     2461 gtttgaaacc ttgctaaaat caatggcatc caaagccagg ctggtgtgaa gttcacgagg
     2521 tttcccagtc tcgtctagcc tgtcccaatc ataaattcga taggtaatat cggatgtttg
     2581 ctgtatttcg agtagcaaaa caccccgccc aatagcgtga actctacctg caggaatgta
     2641 gtaaacatcc cctgcaaggg ctgtttccac gttgaggagg tcggtaagtt tcccagtcga
     2701 taggctctcc aggtactcct ctcgtgtaac atcccggtta aatccggtaa tgattttagc
     2761 gccaggatcg gcatctatca cataccacat ctcggttttc ccgtaggcat ggtgccgttc
     2821 tcttgccagc tcgtcgtttg ggtgtacctg gatggatagg ttgtcggagg cgtcaatgaa
     2881 ctttaccagg agcggaagct ctatcccaaa cttttcataa accgaattgc ctaccagatc
     2941 ccccatgtat atttcaacca gctcctccag gttgttcccc tccaggaagc cgttggctat
     3001 taccgaaata ttatcgccaa ctgccgatac ttcccaactc tcgccacaat ttgaaatatt
     3061 cttgggaatg tttttgttta gcctttgccg aagcttgtta ccgccccata ttctaggctt
     3121 gaatatcggt ttaaacctga gtggatacaa acccgtgttc tccatgataa aagttttcct
     3181 attcgattac aaattaattg aaaattttta acttcgttag tttacctttg tctattattt
     3241 attaacagcc atcgtatgct tattgttgga atagccggcg ggacaggatc tggaaaaaca
     3301 accgttgtca accgtattat agacctgttg cctaagggcg aagtggttgt tctgccccag
     3361 gacgcatact atcgtgatag ctcacatttg cccttggagc aaaggcagga gataaatttt
     3421 gatcatcccg attccattga gtttgagctg ctggtagaac atgtaaaatt gctaaagga
//
LOCUS       DAOOQX010000140         3479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_32297_length_3479_cov_2.165304, whole genome shotgun sequence.
ACCESSION   DAOOQX010000140 DAOOQX010000000
VERSION     DAOOQX010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32297_length_3479_cov_2.165304"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..657
                     /locus_tag="PLX53_03180"
     CDS             304..657
                     /locus_tag="PLX53_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72113.1"
                     /translation="MKLFLILLGWVTITLLIPAALHFSLPVVPFDDFHFHTDYLVHFL
                     IFAMLTAAASLANLRIKPVILFPFFLATAALVEVCQLKIPSRTYNPYDLICNLSGVAF
                     SLIVFYKRFKNHHSD"
     gene            714..1100
                     /locus_tag="PLX53_03185"
     CDS             714..1100
                     /locus_tag="PLX53_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOY72114.1"
                     /translation="MDGKEKILYVDDETINLELFKINFRNEYDVVVANSAIKGLEILK
                     GEPIKVVVSDLKMPVMNGLEFIEKVKGDNPARVCILLTAFMESDVMLQAINKELIFRY
                     IMKPWKKAELEEVIRLALVRHASLAG"
     gene            complement(1089..>3479)
                     /locus_tag="PLX53_03190"
     CDS             complement(1089..>3479)
                     /locus_tag="PLX53_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR/HmsC family protein"
                     /protein_id="HOY72115.1"
                     /translation="GVMDADTILLHELHKVESSYLIRGKPFNTFYFSNESQLENIDIL
                     YLSSEFSKNLFRVYRELEGRPTLMITDGASDRALTMINLTYNAEKKAIAFEVNRENME
                     SHGFRVGQELLLLGGSYMDIKELYVETSKRLNEATSLMAKYQEELDQLGRDHDEYLHQ
                     IEMLDSQLTTLNYQLKKTEQDYSNLTLRLHDRDSLLGVRNRELQLRINESTLLQGLIQ
                     TQLDSIKNSSTLLDSLNQQVAEKQNELLAQQNLINNQGQVITEKESTILRQQRKWYIT
                     LILLLGVLSSLIFASISYTTKRRMNLRLENLVEERTRELNASREHFKNLFENSPVAMI
                     ELNFSALKDFSWQSDNGKVDIDSMSNEEFSALMSRALTYSRVVDANNAAIKLFGYADK
                     KDFMENPSKTLTFQSLVDYRSLAKLYFNNQPLAKFETTRQTTSGETIHAVFEWLVLPG
                     YENTYERVLLAITDITALKKYEKELTLHKDHLEEIVLERTREIIKLNEDLTSTNAELK
                     QKTEELTQTIQKLGEAQNQLVRSEKLASLGMLTTGIAHEINNPLNYISGSQQALGTLL
                     EEMWKLLNSYRDIIATKLTSDFLINIEKEHNQNINDLYFSMKFLLSNIETGISRANSI
                     VKSLRVFLGESSQEMAEYSIPNAVGSILTILKSSYFGKIRITEEYSPDLPLIHCSAEG
                     VNQVIMNLLTNAIDAIPEKGEINITATYEVDDDCIAVQVRDNGMGISKENINRIFDPF
                     FTTKETGSGTGLGLYLAYNIVIAHHGSIEVDSAEGVGTTFTLRLPRMVPNGLELPS"
BASE COUNT          829 a          752 c          842 g         1056 t
ORIGIN      
        1 ttgcgggtca ggcttttatg ccgttttact tataccagca acgttcaacg attcctgcct
       61 ttacgagcat tgcagagaat gctttccccc gttggggctg aagaacgagg ataaaggttg
      121 atgggaagaa atcccccatg aataagctaa cggtaaaagc ccacaacagg ctaagtgttt
      181 aggaaacagg gttttaaagg tgacaaatat ttacttgtta ccgctgattt tacctgtgct
      241 gctaccaaaa tgtatttttt tcgtcatatt tgagccatga aaaatttatg gcgtgtaaag
      301 tgggtgaagt tattccttat actgttaggt tgggttacca ttaccctatt aattcctgca
      361 gctttacact ttagccttcc ggttgttccc tttgatgatt ttcatttcca taccgattac
      421 ctggtgcatt ttttgatttt tgcgatgttg actgccgctg cttcgctggc caacctacgc
      481 attaaaccgg taattctatt tccttttttt ttggcaacag cagccttggt ggaagtttgc
      541 cagctgaaaa tcccatcgcg tacctacaac ccgtacgact taatttgtaa cctttccggc
      601 gtagcctttt cattaatcgt cttttataaa aggtttaaaa atcatcattc cgattaacct
      661 tgtaagctaa attttagtaa atttgtttat attgataaaa actttcgggt aacatggacg
      721 gtaaggagaa aattctttat gttgacgatg aaaccattaa ccttgagctg ttcaagatta
      781 actttcgcaa cgagtatgat gtggtagtgg ccaactcggc catcaagggg ttggaaattt
      841 taaaaggtga accgataaag gtggttgtgt cggacttaaa gatgcccgtg atgaacgggc
      901 tggagttcat tgaaaaggtt aagggcgata accccgctcg ggtttgcata ctgcttaccg
      961 ccttcatgga atcggatgtt atgcttcagg ctattaataa ggagttgata ttcaggtata
     1021 taatgaaacc ttggaaaaaa gccgagctgg aggaggtaat caggttggcg ctagtccgcc
     1081 acgcgagcct agctgggtag ctccaacccg tttggtacca tgcgtggcag ccgaagggtg
     1141 aaggttgttc caactccttc agcagaatcc acctcgatgg agccatgatg tgcaatcacg
     1201 atgttatatg ccaggtaaag ccctaaccct gttccgctgc cggtttcctt ggttgtgaag
     1261 aaggggtcga atatccggtt gatattttcc ttcgatattc ccatgccgtt atccctaacc
     1321 tgcacagcta tgcagtcgtc gtcaacttcg taggtagctg tgatatttat ttcgcccttt
     1381 tcgggaatag catcaatggc attagtgagc aggttcatga taacctggtt aaccccctct
     1441 gcactacagt ggatgagtgg caggtctggg gagtactcct cggtaattct gatcttacca
     1501 aagtaggatg attttaaaat ggtgagaata cttccaaccg cattggggat gctatattcg
     1561 gccatttcct ggctgctttc acctaaaaac accctcaggc ttttaacgat ggagttggcc
     1621 ctgctgatgc ccgtttcaat attggagagc aggaatttca tggagaagta aaggtcgttg
     1681 atattctggt tgtgctcctt ttcgatgttt attaaaaagt cgctggtaag cttggttgca
     1741 ataatatcgc ggtagctgtt aagcagcttc cacatttcct ccagaagtgt tcctagtgcc
     1801 tgctggctgc cgcttatgta gtttaggggg ttgttaattt catgggctat tcccgttgtg
     1861 agcataccca gcgaggccaa tttttctgaa cgtacaagtt gattctgcgc ttcgcccagc
     1921 ttttgaatgg tttgtgtaag ctcctcggtt ttctgcttca gctcggcgtt ggtggaagtc
     1981 aaatcctcat tcagcttgat aatctccctg gtccgttcca gaacaatttc ctccagatga
     2041 tctttgtgca gggtaagctc cttctcatac tttttaaggg cagtaatatc ggtaatagcc
     2101 aaaagaaccc tctcgtaggt attttcgtag ccgggaagca cgagccattc aaaaacagca
     2161 tgtatagttt cgcctgaagt ggtttgcctg gtagtttcaa actttgcaag gggctggtta
     2221 ttaaagtaga gtttggccag cgaccggtaa tcaaccagcg actggaaggt tagcgttttg
     2281 gagggattct ccatgaagtc ctttttatcg gcatagccaa aaagtttaat ggcggcgttg
     2341 ttagcatcaa ccaccctgga gtaggtaagg gcacgggaca taagtgctga gaactcctca
     2401 ttgctcatgc tgtcgatatc caccttgcca ttatcggatt gccatgagaa atctttcagg
     2461 gccgaaaagt tcagctctat catggctacg ggggagtttt caaataaatt tttaaaatgc
     2521 tcccggctgg catttagctc ccgggtgcgc tcctcaacaa gattctcaag ccgcaggttc
     2581 attctccgct tggtggtgta tgaaatagag gcaaaaatca ggcttgataa aacacccaaa
     2641 agcaaaatca gggtgatgta ccatttccgc tgctgccgga gaatggtgga ctccttttcc
     2701 gtgattacct gcccctggtt gttaatgagg ttttgctggg ccagcagctc attttgtttt
     2761 tcggctacct gctggtttag cgaatcgagg agggttgaag aatttttaat ggaatcgagc
     2821 tgggtttgaa ttaacccttg cagcagggtg ctctcgttaa ttcttagctg gagctccctg
     2881 ttgcgaacgc ccagcaagct gtccctatcg tgcagcctta gggttaagtt gctgtagtcc
     2941 tgctctgttt ttttgagttg atagttcaga gtggttagct ggctgtctag catctcaatc
     3001 tgatgaaggt actcatcgtg gtcgcgtcca agttgatcca gctcttcctg gtatttggcc
     3061 atcagggagg ttgcctcatt cagccgtttt gaggtttcca cgtatagctc cttgatatcc
     3121 atgtagcttc cgccgagcag aagcagctcc tgcccaaccc tgaagccgtg tgattccatg
     3181 ttctcgcgat taacctcaaa ggcaatagcc tttttttctg cattgtatgt aaggttaatc
     3241 atggttagcg ccctgtcgga ggcaccgtcg gtgatcatta gggtaggcct gccttcaagc
     3301 tcccggtaaa ctctaaaaag gtttttgctg aattctgatg aaaggtagag tatgtcgatg
     3361 ttctccagct ggctctcatt ggagaagtaa aaagtgttaa agggcttccc cctgatgagg
     3421 tatgaggact caaccttatg tagctcgtgg aggaggatgg tatcggcatc catcacccc
//
LOCUS       DAOOQX010000141         3464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_32484_length_3464_cov_2.639190, whole genome shotgun sequence.
ACCESSION   DAOOQX010000141 DAOOQX010000000
VERSION     DAOOQX010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32484_length_3464_cov_2.639190"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..696)
                     /locus_tag="PLX53_03195"
     CDS             complement(28..696)
                     /locus_tag="PLX53_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72116.1"
                     /translation="MKRSAFFILVHLAAIFSCRAQGKVETDSSATMSIVRYATDLRCL
                     DGENAELLVNVLYEFNGLPPINRIELELPEAKEKVFRQIFVEVSSNDKKFIFRRKIRK
                     PDADELSALDPCALASKYSNLKRPVTVEWEIRVSLRSPAGSYKWPSIATGNFERIDHL
                     SFRVAYGKELSADIQTNLTTDPQKDTINGNFYYLWEFNDVNPSRTELKGFHSHDPYVI
                     LNFR"
     gene            complement(770..2170)
                     /locus_tag="PLX53_03200"
     CDS             complement(770..2170)
                     /locus_tag="PLX53_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY72117.1"
                     /translation="MEKIRETLRDSAVARWTALALVAFTMLTGYYLTDVMAPLKGLLE
                     EQLTWNSTEYGIFTSAYGWFNVFLLMLIFGGIILDKMGVRVTGLGAAAVMITGTAIKF
                     WAVSTHSLDGQVWNILWLFPMKAQVFMAALGYAVFGVGVEVAGITVSKIIVKWFKGKE
                     LALAMGLEMATARMGTALALATSVPIAKSLGHVSKPILVCLIMLCIGFISFFIFTFMD
                     RKLDASQAAYDKDNNVQNDEEAFKVRDIFKIITNRGWWYIAILCVLFYSAVFPFLKYA
                     SDLMVNKFGISENLAGTIPAMLPFGTILLTPLFGNLYDRKGKGATIMLIGALLLILVH
                     SFFSLPFLTHWIVAIILIIILGIGFSLVPSAMWPSVPKIIPERQLGTAYSLIFWVQNW
                     GLMGVPALIGWVLDKYCVTGERITDSGLTVHTYNYTIPMLIFTSLGILALVFAFLLKA
                     EDKKKGYGLELPNIAK"
     gene            complement(2337..2801)
                     /locus_tag="PLX53_03205"
     CDS             complement(2337..2801)
                     /locus_tag="PLX53_03205"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOY72118.1"
                     /translation="MKKLTCVLILLTLLGSGCKFVEKYTKKGKKAKAEAMQKAKEDSL
                     AKAKAIEFEKAALAREQAHQDSIRQVEEYENQHRFHVIIGSFKVPSNATSWEQEVRGM
                     GFNNTRILQAPNGFNLVSVGAFNTYSKAFNEIDRINSNRIENPMEMWVYEKQ"
     gene            complement(3019..>3464)
                     /locus_tag="PLX53_03210"
     CDS             complement(3019..>3464)
                     /locus_tag="PLX53_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72119.1"
                     /translation="YLPDFKYVNPELGLRYSGVADYPDVAAKAIREMYRQKGSSLLVD
                     NDGLAESGMIIRHLVLPGYADESIRLLKFLAEEFTPRIYLSLMSQYYPPVGVELQSPL
                     NRTVTMEEYMGVVEVAQGIGFRGWIQDLESFGSYQPDFSRSNPFE"
BASE COUNT          981 a          805 c          736 g          942 t
ORIGIN      
        1 gttagagcct gaccaaccct tcttttgtta gcgaaaattt aggataacat acggatcgtg
       61 gctgtgaaag cctttcaact ccgtacgtga agggttaaca tcattaaact cccagaggta
      121 gtaaaaattc ccatttatag tatccttctg gggatcagtg gtaaggttgg tttgaatgtc
      181 ggcagaaagt tccttgccgt atgctaccct gaagctaagg tggtctatcc tctcaaagtt
      241 acccgtagca attgatggcc atttgtagct gcctgctggt gagcgaaggc ttactcttat
      301 ttcccactcc acggttacag gcctttttaa attactgtac tttgaagcca atgcgcaggg
      361 gtcgagggcg gataactcat cggcatcggg cttgcgaatt tttcttcgaa aaataaactt
      421 cttatcatta ctgcttacct ctacgaaaat ttggcgaaac accttctctt tagcctcggg
      481 cagctccagc tcaattctgt ttatgggggg caggccgttg aattcgtaga gaacgttcac
      541 cagcagttcg gcattctctc catctaaaca tcttaaatcg gtagcatagc gaactatgct
      601 catggtagcg gagctgtcgg tttcaacttt tccctgagcc cgacaagaaa aaatcgctgc
      661 caggtgaaca agtataaaaa atgcagatcg cttcattaaa cttttctaat atgcgttaaa
      721 acgcccttgt tgggggcgtt tcaacgcatt agtccattac ataagctgac tacttagcta
      781 tattagggag ttccagaccg taaccctttt tcttgtcctc tgctttaagc agaaaagcaa
      841 acaccaacgc cagaattccg agcgaagtaa atatcagcat gggaatggtg tagttgtaag
      901 tatggacagt aaggccactg tcagtaattc tctctccggt tacacagtac ttgtcgagca
      961 cccagccaat taaagcagga acgcccatca acccccagtt ttgaacccaa aaaatcaagg
     1021 agtaagctgt gccaagctga cgttcgggta taattttggg tacagatggc cacatggccg
     1081 agggcaccag cgaaaatcct attcccagaa tgattataag gatgatggcc actatccagt
     1141 gagtaaggaa tggtaatgag aaaaaggaat gaaccaggat aagaagtagg gcgccaatca
     1201 gcataatagt agcacctttc ccctttcggt cgtataagtt accgaagaga ggggtaagca
     1261 gaattgttcc gaatggaagc atggctggaa tggtgccggc aagattttcg ctaattccaa
     1321 acttattgac cataaggtcc gacgcatatt taaggaatgg gaaaacagcg gagtagaaaa
     1381 gcacacaaag aatggcaata taccaccacc cgcggttagt aattattttg aaaatatcac
     1441 gaaccttaaa tgcctcctca tcgttctgca cgttgttatc cttgtcgtag gctgcctggg
     1501 aggcatcaag ttttctatcc ataaaggtga aaataaagaa tgaaatgaag cctatgcaaa
     1561 gcatgattag gcaaaccaaa atgggttttg agacgtgtcc tagagatttt gctattggaa
     1621 ccgatgtggc tagtgccaga gcagttccca ttcgggcagt agccatctcg agccccatgg
     1681 caagtgcaag ttctttcccc ttaaaccatt tcacaattat tttcgaaacg gttattcctg
     1741 ccacttcaac gccaactcca aaaactgcat atcccagcgc agccataaaa acctgagctt
     1801 tcatcggaaa caaccagagt atattccata cctgcccatc aagcgaatgg gttgatacag
     1861 cccaaaactt aatagcagtt ccggtaatca tcaccgctgc tgctcccagg ccagtaaccc
     1921 ttacccccat tttatccaga ataatgccac cgaaaatcag cattagcaaa aaaacattaa
     1981 accaaccgta agcactggta aatattccat actccgtgct gttccatgta agctgctctt
     2041 ctagcaagcc cttaagcgga gccattacgt cggttaggta gtaaccggtt agcattgtaa
     2101 aagcaacaag agccaaagcc gtccatcgtg ctactgcaga gtcacgaagt gtctctctaa
     2161 ttttttccat tctctataaa ttaatttaat tattgatttt taagggttga aaaaaatttc
     2221 aaacgaacgc aaataaactt aaatccaatc taaaatccta tgagattgac caatatttga
     2281 aaataaaaaa ccgtcatgaa aatgatgacg gctaaagagt ttagggaatg aagcagctac
     2341 tgtttttcgt aaacccacat ttccatagga ttctctatcc tgttggagtt tatccggtca
     2401 atttcgttaa aagccttgct gtaggtattg aaggcaccaa ccgatactag attaaagccg
     2461 tttggcgctt ggagtattcg ggtgttgttg aaccccatac ccctgacttc ctgctcccag
     2521 ctagtggcgt tggatggaac cttgaagctg ccaataataa catgaaaccg gtgctggttt
     2581 tcatattctt ctacctgcct gatggaatcc tggtgtgcct gctcccttgc cagagcggcc
     2641 ttctcaaatt ctattgcctt tgccttggca aggctatctt cctttgcttt ctgcatggct
     2701 tcggctttag ctttcttgcc tttcttggta tatttttcaa caaacttgca tccgcttcct
     2761 agcagggtca gcaaaattag cacacaggta agttttttca tttggagttt gggttagttg
     2821 tatatttcaa tagttgtgca agttagcaaa agttatgcta aaaatgcaag tgcaatccct
     2881 ttaaaatttc cccgtccttc caattttatt cctaagggtt ccgattcttc atgttaaaaa
     2941 agtgtgttaa cacagcggaa gggtaaaccg atagcccggc tggatgggtt tcttccattt
     3001 aataaattat agctttacct actcaaaagg gttactccgt gaaaaatcgg gttggtagct
     3061 tccgaaactc tcgaggtcct gaatccagcc cctaaaaccg attccctgag caacctctac
     3121 tacccccata tattcctcca ttgtaaccgt tctgtttaga ggggactgga gctccactcc
     3181 cacgggggga tagtactgcg acatcagcga gaggtaaatg cgcggcgtaa actcttcggc
     3241 aaggaatttg agtagcctaa tactctcatc ggcatagccg ggaagcacca agtggcgaat
     3301 aatcataccc gattcggcca aaccatcgtt gtcaaccagc agcgagctcc ccttctgacg
     3361 gtacatctcg cgaatggctt ttgccgcaac atccgggtag tctgccactc ccgagtagcg
     3421 caagccaagt tcagggttaa catacttaaa atcggggagg taaa
//
LOCUS       DAOOQX010000142         3439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_32808_length_3439_cov_2.487293, whole genome shotgun sequence.
ACCESSION   DAOOQX010000142 DAOOQX010000000
VERSION     DAOOQX010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32808_length_3439_cov_2.487293"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /gene="mnmA"
                     /locus_tag="PLX53_03215"
     CDS             <1..992
                     /gene="mnmA"
                     /locus_tag="PLX53_03215"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOY72120.1"
                     /translation="LRIPFEFVDLSNEYRQRVVDYMFNEYSKGRTPNPDVLCNREIKF
                     DAFVKAAFELGADYVATGHYCRKQEIEVNGQTYYRLLTGTDSNKDQSYFLCQLSQQQL
                     SRALFPIGHLLKPEVRKIASELGLATAERKDSQGICFVGKVDLPVFLQQKIKAVDGDI
                     VEIPRAFYSSQPVISPDDLEALCKPTFYSKADGNLVGQHHGAQFFTIGQRKGLNVGGK
                     SEPLFVIATDVLQNVVYTGMGDDHPGLYRKGLFVSQNDVHYIRPDQKLRPGQTARLMV
                     RIRYRQPLQKATLLSRDNGIYIIFDNPQRGVTAGQFAAWYDNEELIGSGVIAM"
     gene            1124..1759
                     /gene="msrA"
                     /locus_tag="PLX53_03220"
     CDS             1124..1759
                     /gene="msrA"
                     /locus_tag="PLX53_03220"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOY72121.1"
                     /translation="MKRVAIVASWLLITQFSMACESQTNQNHKEMKASASETITLGAG
                     CFWCVEAIFSRIDGVLKVESGYSGGSAVNPTYREVCNGNTGHTEVVQVTFDPEVIPLA
                     KILEIYFKTHDPTTLNRQGADVGFQYRSVIFYHSQEQKKIAEEIKAELEKARIWNSPV
                     VTAIEPFKNFYKAEDYHQEYYENNKNQPYCRMVITPKVDKLEKVFADYLKH"
     gene            2026..2796
                     /locus_tag="PLX53_03225"
                     /pseudo
     CDS             2026..2796
                     /locus_tag="PLX53_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444759.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
     gene            complement(3039..>3439)
                     /locus_tag="PLX53_03230"
     CDS             complement(3039..>3439)
                     /locus_tag="PLX53_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOY72122.1"
                     /translation="HGLSYGLTLGVDIFDGFNRKRERTNAAIEIENREHEYSDVVQGV
                     KADLLTIYYAYQNNLKLLKLEEQNLSVARENLEIAMERYRLGDLSGLELREVQKSLLD
                     AEERLISVKFQAKLAEISLFQISGKIMSYL"
BASE COUNT         1008 a          764 c          737 g          930 t
ORIGIN      
        1 agctaagaat tccctttgag tttgttgact taagcaacga atatcgccag cgtgttgttg
       61 actacatgtt taacgaatac tcaaagggtc gcacgcccaa tcccgatgtg ctatgcaacc
      121 gggagataaa atttgacgcc ttcgtgaaag ccgcattcga gctgggtgct gactatgtgg
      181 ctaccggtca ttactgccgt aagcaggaga tagaggttaa tgggcaaaca tactaccgac
      241 tattgaccgg aaccgatagc aacaaggacc aaagctactt cctgtgccag ctttcacagc
      301 aacagctcag cagggcattg ttccccattg gacacctgct caagcccgaa gtgcgaaaaa
      361 ttgcttctga gctgggtttg gctactgccg aacgtaagga ttctcaagga atttgctttg
      421 ttggcaaggt tgacctgcct gtttttcttc agcaaaaaat taaagcagtt gatggcgata
      481 tagttgaaat tcccagggca ttttacagct cccaaccggt gatttctcct gatgatctag
      541 aagcactctg caagcccact ttctacagca aagccgacgg gaacctcgtt ggacaacacc
      601 atggggctca attctttacc ataggccaac gcaaaggctt aaatgtgggg ggaaaatcag
      661 aacctctttt cgttattgcc actgatgttc tgcagaacgt ggtttacacg ggtatgggtg
      721 acgatcaccc cggcctatac cgtaaaggtc tgtttgtcag ccaaaacgac gttcactaca
      781 tccgtcccga ccaaaagcta aggcccggac aaacagcaag gttgatggtt agaatacgct
      841 accggcaacc actccagaaa gctacgctgc tttccaggga taatggaatc tatatcattt
      901 ttgataaccc ccaaagaggg gttacagcgg gacaatttgc tgcatggtac gataacgagg
      961 agctaattgg ttccggtgtg attgccatgt aggttgcaac ccgaattcac tcaccccctt
     1021 tacctcaaat agggaatcga gctgctggcc ataactatgc aatccaacct gctgcaaaac
     1081 aaaataaggc atgtagctgt ttactggtta aaattactac acgatgaagc gagttgccat
     1141 tgttgcatca tggttgctga ttacgcaatt ttccatggca tgtgaaagtc aaaccaacca
     1201 aaaccacaaa gagatgaaag caagcgctag cgaaactata accctcggtg caggatgttt
     1261 ttggtgtgta gaggcaatat ttagcaggat tgatggggtt ttgaaagtgg aatcggggta
     1321 ctcaggtggc tcagcagtaa acccaaccta cagggaagtc tgcaacggta ataccggtca
     1381 caccgaggtt gttcaggtta ctttcgaccc tgaggtaata cccttggcta aaattttgga
     1441 aatctacttt aaaacccacg accccacaac cctcaacagg caaggggcag atgtaggatt
     1501 tcagtaccgc tcggtaattt tttaccactc ccaggagcaa aaaaaaatag cggaggaaat
     1561 taaagctgag ctggaaaaag caaggatatg gaattcacct gttgttacgg ctattgaacc
     1621 gtttaaaaac ttctacaagg ccgaagatta ccatcaggaa tactacgaaa acaacaagaa
     1681 ccaaccctac tgtcggatgg tgataacccc aaaagttgac aaactcgaga aggtttttgc
     1741 cgattacctg aaacactgaa agggcaaaga aactccacgg ggaaccacta gccagaacat
     1801 tcctgcgtta tcatttattc cgtttctaaa aaagcaaacg ggtaaaaaaa tcatgccctc
     1861 aggcatggca gccatgtagg catatccatc ataaatacac cctgctggca ttaaccctta
     1921 gggataccgt tttcaaaaaa tttccggtaa aaggattcaa ttatggaaaa tgagcctact
     1981 tttgcaggct aaaagtaaaa agcactcaac caatcctgaa agacgatgag ttttatacaa
     2041 tcaattatca tagctattgt tgaaggaata acggagtttc tacccgtgtc gtcaaccggc
     2101 cacatgattc tggcttcaac attaatgaaa attcaggaaa atgattttgt aaaaaccttt
     2161 gaaatattca ttcagctagg cgcaataatg gccatcgtaa tgctttacgc caaaaagttt
     2221 ttgcaaagtt tttctattta tgtaaagctt ggaatagcat ttattcccac tgcggtggta
     2281 gggtaaatag cctacgactt tatcaaagga tatctattca actctattgt agttgcagtt
     2341 tcactaattg ttggaggaat tgttctggtg cttattgacc aaaagcttac tacaaaaaag
     2401 agtaaagttg aggtggtgga agatatttcg tataaaaatt cttttttcat aggattaatt
     2461 cagtgcttct ccatgattcc cggagtgtca agggcagcag ccacaattat tgggggagtt
     2521 tttaacggac ttgacaaaaa acaggcaaca gagttctctt ttctacttgc cgttccaacc
     2581 atgattgccg cctctgggta tgatttgctg aaaactccaa ttagctttac ctctcacgag
     2641 gttgttttgc tggcaacggg atttgtggta gcctttttta ccgcatggct tgccgtaaaa
     2701 gtcttcttaa aaatagtaga aaattacggc tttaagtatt ttggctacta ccggatactc
     2761 gtaggcgcta tttttttgct gttttttagg ttttagcaaa ccagcagctc cccccctgca
     2821 tggtaatagc cttagactct acaaaacaag aaatgctctg aaaggatagc tatacctacc
     2881 gggtgttttc catgggtgtt actgctaaca ccctttataa gcagcattct accaaacctt
     2941 tatcctgata attaacaggt taactcaaaa ttcaacctgt aaagccgatc gatctgccag
     3001 aacgtctttg atcatgccac agccctggaa gctgaaaatt aaaggtagct catgattttc
     3061 cctgaaattt ggaatagaga aatttcggct agctttgcct ggaacttaac ggagataagc
     3121 ctttcttcgg catcgagtaa acttttctgg acttccctta gttcaagccc cgacaaatcg
     3181 cccagcctat agcgttccat ggcaatttcc aggttttcac gggctacaga aaggttttgc
     3241 tcttcaagtt tcagcagctt gaggttgttc tgataggcat agtatatggt tagcaagtcg
     3301 gcttttactc cctgcaccac atcgctatac tcatgctccc gattttctat ttcaatagct
     3361 gcgtttgtcc gttcgcgctt gcggttgaag ccgtcaaaaa tatcaactcc aagagtaaga
     3421 ccatagctta agccatgaa
//
LOCUS       DAOOQX010000143         3418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_33094_length_3418_cov_2.867975, whole genome shotgun sequence.
ACCESSION   DAOOQX010000143 DAOOQX010000000
VERSION     DAOOQX010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33094_length_3418_cov_2.867975"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PLX53_03235"
     CDS             <1..407
                     /locus_tag="PLX53_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72123.1"
                     /translation="LTKEGLSNILDAVKASRRIYQRMLTYTLNKIIKTFQIALFLSVG
                     FVLTREFVVTPLLIILLLFANDFVTMSLATDNVSYSRKPDRWKILPQTPIPMEVIIRP
                     GDNSTVVPALEFSTPIKKSALSMLMFLNILNP"
     gene            473..895
                     /locus_tag="PLX53_03240"
     CDS             473..895
                     /locus_tag="PLX53_03240"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOY72124.1"
                     /translation="MEKALDMNTQVYAVGSYNFICIGSFSSKSDAIESFGKVVDLNFP
                     QAVIINENELQRIVRSSQKQLITKRTIYTILVVTCEKPLNQDSFKGFPVTEEKGEDGL
                     YRYYFGIFDTRDSAKKNLDTVNSLGFPNAIIVKLEKNR"
     gene            895..1461
                     /locus_tag="PLX53_03245"
     CDS             895..1461
                     /locus_tag="PLX53_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY72125.1"
                     /translation="MTEGVLENNMVKLRAPEPDDVDLLYVWENNMEIWKVSNTITPFS
                     RHLLKKYIETAHLDIWESKQLRLIIEAKDQNALMNVPVGLIDLFDFDPYHLRAGIGIL
                     IANQQYRRRGYATEALKLLVRYCFGILQLNQIYCNISADNQVSLQLFKKQGFVEVGVK
                     KSWLKTQSGWEDEVMLQLLNPKPRLPKN"
     gene            complement(1526..2989)
                     /locus_tag="PLX53_03250"
     CDS             complement(1526..2989)
                     /locus_tag="PLX53_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOY72126.1"
                     /translation="MSQTEKYFEQFRKNIVGIDTTYPTVFGQKPLIYADWIASGRLYR
                     PIEERMLNVFGPMVANTHTESSYTGKFMTEAYHFALKEIKRHVNAGSDDVIVTSGFGM
                     TSSVNKFQRILGLKNYSFVFANPKERPVVFVTHMEHHSNQTSWYETIAEVVVVEPDEN
                     LMVSPANLRRTLENYSDRPMKIGSFTACSNVTGIRTPVHELARIMHEVGGLCFVDYAA
                     SAPYEEINMHPSEPLERLDAIFFSPHKFLGGPGASGVLIFNSQLYKNRVPDNPGGGTV
                     DWTNPWGEYKYIDDIERREDGGTPGFLQAIRSALAIRLKEKMGVKNIALREEELVNLA
                     FEKLKGIPGLHILAENCCQRIGSISFYIDELHYNLVVTLLSERYGIQMRGGCVCAGTY
                     GHFLLNVTKDKSHRITEMINHGDFSQKPGWVRWSLHPTTTDAEVEYIANALREISANG
                     TSWKNDYTYCKHTNEFCSKNPEVKSLWPDIPSLFNFD"
     gene            complement(3020..>3418)
                     /locus_tag="PLX53_03255"
     CDS             complement(3020..>3418)
                     /locus_tag="PLX53_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOY72127.1"
                     /translation="QIFDAVRKKFVPLTPEEWVRQHLIRYLCDFKNVPTGLIGVEVAF
                     LLNGVNHRADVVVYGRAGKPIVVVECKAADVELSESTVDQVVRYNSYFKAPYLVITNG
                     IKHYTFAVSWESGNSYSLNEIPDYSVMEQS"
BASE COUNT          949 a          853 c          751 g          865 t
ORIGIN      
        1 tgctgacaaa agaggggtta agtaatatcc tggacgcagt caaggccagc cgccggattt
       61 accaacgcat gttgacctat acactcaaca aaattatcaa gactttccag attgcactgt
      121 tcctgagcgt gggatttgtt ttaacccgcg aattcgtcgt cacgccgctg ttaattatcc
      181 tgctgctgtt tgcaaatgat tttgtcacca tgtcgcttgc cactgacaat gtctcctatt
      241 cacgcaaacc ggatcgctgg aaaatcttgc cacaaacacc aatcccgatg gaagtgataa
      301 tccggccggg agacaattca accgtcgtgc cagcattaga attttcaacc ccgataaaaa
      361 aatcagcatt gagtatgttg atgttcctga acatcttaaa cccataaact ctcgatatac
      421 catactacta gcccaacccg gtgttaagcc tacagaaaaa cagatttcag aggtggagaa
      481 agccctggac atgaatactc aggtctatgc ggttggttcc tacaacttta tatgcattgg
      541 cagcttctct tccaaatccg atgccatcga atcgttcggg aaggttgttg acctcaactt
      601 cccacaagcg gtaattatca acgaaaatga gctgcagcga attgtcagat cctcgcaaaa
      661 gcagctaatc accaaacgca ctatctacac catactggtg gtaacctgcg aaaagccgct
      721 aaaccaagac tcattcaaag ggttcccggt aacggaggag aaaggcgaag acggtctgta
      781 caggtactat tttggcatct ttgacaccag ggactctgcc aaaaagaatc tcgacaccgt
      841 taactctctg ggatttccga atgcaattat agtgaagctc gaaaagaaca ggtgatgact
      901 gaaggtgttt tggaaaacaa catggtaaaa ctcagggcac cggaacccga tgatgtggat
      961 ttgctctacg tttgggaaaa caacatggaa atatggaagg tgagcaacac cattacccca
     1021 ttctcacgcc acctgctaaa aaaatacatc gaaacagcac acctcgacat ttgggagtca
     1081 aaacaacttc gactcatcat tgaggctaag gatcagaacg ccctgatgaa tgttcctgta
     1141 gggttgattg acctttttga cttcgacccc taccatctta gggcagggat aggtattctg
     1201 attgcaaacc agcagtaccg tcgcagaggc tatgctactg aggcactaaa actcctcgtt
     1261 cggtactgtt ttggcatcct ccagctaaac cagatttact gcaacatatc ggccgataac
     1321 caggttagcc tgcagctctt caagaagcaa ggattcgttg aggttggggt aaaaaaatca
     1381 tggttaaaaa cgcaaagcgg ctgggaggat gaggtaatgc tccagctcct taaccccaaa
     1441 ccacgcctac caaaaaacta acccggaata aaagctgcca gggttcaaac cacaagctgc
     1501 acgagtaaaa aattccgagt tgcaattaat caaagttaaa aaggctcgga atatctggcc
     1561 atagagactt aacctctgga tttttgctac agaactcgtt ggtgtgctta cagtaggtgt
     1621 agtcattctt ccacgatgtg ccgtttgctg aaatttcccg aagcgcattg gcaatgtact
     1681 ccacctcggc atcggtggtt gttggatgta gcgaccacct aacccagccg ggcttctgag
     1741 aaaaatcgcc atgattaatc atttcggtaa tccggtgcga tttatcttta gtaacattca
     1801 acaggaaatg gccgtatgta ccggcacaaa cacacccgcc ccgcatttga atgccgtagc
     1861 gctcgcttag cagggtaacc accaggttgt agtggagctc atcaatgtag aatgaaatag
     1921 agccaattct ttggcagcag ttttcggcca aaatatgaag cccggggata cccttcagct
     1981 tttcgaaggc aagattcacg agctcctcct cgcgaagggc gatattttta acccccattt
     2041 tctccttaag cctgatggcc agtgcagagc gaatggcctg aaggaaaccg ggcgttccac
     2101 catcctcccg cctttcgata tcgtcaatat acttgtactc accccagggg ttcgtccagt
     2161 ccaccgttcc gcctccaggg ttatcgggaa ccctgttttt ataaagttgc gagttgaaaa
     2221 ttagcacgcc cgaagccccg ggcccaccaa gaaacttgtg gggtgaaaag aatatggcat
     2281 ccagcctctc aagtggttcg gaggggtgca tgttaatttc ttcataggga gcagatgcgg
     2341 cataatcaac aaaacaaagg cctccgacct cgtgcatgat acgcgccaac tcgtgaacag
     2401 gagttcggat accggttaca ttgctgcaag ccgtaaagga accaatcttc attggcctgt
     2461 cgctgtaatt ctcaagtgtt cttcttaaat tagctggtga aaccatgagg ttctcgtcgg
     2521 gctccactac caccacctcg gcaatagttt cgtaccatga ggtttggttg gagtggtgct
     2581 ccatgtgggt aacaaacacc accggccgct ctttagggtt ggcaaagaca aagctgtagt
     2641 tttttaaccc caggatgcgc tggaatttat ttactgagga tgtcattccg aaacccgaag
     2701 ttacaattac atcatccgaa ccagcattta cgtgccgttt tatctccttt agggcaaagt
     2761 ggtaggcctc ggtcataaat ttcccagtgt agctgctctc ggtatgggtg ttggccacca
     2821 tggggccaaa aacattcagc atcctctcct ctattggccg gtatagcctg ccactggcaa
     2881 tccagtcggc ataaattaat ggcttctgcc caaaaacagt ggggtaggtt gtatcaatcc
     2941 ctacaatgtt cttcctgaac tgctcaaagt acttctcggt ttggctcatt tcatagtaat
     3001 attttgagaa aaattatctt caactttgct ccatcacgct gtaatcagga atttcattta
     3061 gactgtagga gttccccgat tcccagctta cagcaaaggt gtagtgttta attccgtttg
     3121 taatcaccag gtagggagcc ttaaagtatg agttgtagcg aacaacctgg tcaacagtgc
     3181 tctcgctgag ttcaacatca gcagccttac actctacaac cactatcggc ttcccggctc
     3241 taccatagac caccacatcg gctctatggt ttaccccatt cagcaaaaaa gcaacctcca
     3301 cccctattaa tcccgtggga acattcttaa aatcgcaaag gtagcgaata aggtgttgcc
     3361 tcacccactc ctcaggggtt agaggtacga attttttcct tacagcatca aaaatctg
//
LOCUS       DAOOQX010000144         3399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_33335_length_3399_cov_3.264952, whole genome shotgun sequence.
ACCESSION   DAOOQX010000144 DAOOQX010000000
VERSION     DAOOQX010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33335_length_3399_cov_3.264952"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLX53_03260"
     CDS             complement(<1..386)
                     /locus_tag="PLX53_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOY72128.1"
                     /translation="MSRDLKYTRNIGIMAHIDAGKTTTTERILFFTGKTHKIGEVHDG
                     AATMDWMVQEQERGITITSAATTTFWRYNDQQYQVNIIDTPGHVDFTVEVERSLRVLD
                     GTIATFCAVGGVEPQSETVWRQADRHR"
     gene            complement(420..896)
                     /gene="rpsG"
                     /locus_tag="PLX53_03265"
     CDS             complement(420..896)
                     /gene="rpsG"
                     /locus_tag="PLX53_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOY72129.1"
                     /translation="MRKSKPKKRLILPDPKFGDVMVTRFVNNLMERGKKSVAFDIFYQ
                     SLDIVGQRMKDADKNPLEIWKKALENITPQVEVKSRRVGGATFQVPMEVRPERKISLS
                     MKNLILYARKRSGKGMSEKLAAEIMAAYNEEGAAFKRKEDMHKMAEANKAFAHFRF"
     gene            complement(929..1306)
                     /gene="rpsL"
                     /locus_tag="PLX53_03270"
     CDS             complement(929..1306)
                     /gene="rpsL"
                     /locus_tag="PLX53_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOY72130.1"
                     /translation="MPTIQQLVRKGRSKMVDKSKAPALDACPQRRGVCVRVYTTTPKK
                     PNSAMRKVARVRLTNGKEVNAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHLIRGTL
                     DASGVEGRKQRRSKYGAKRPKKK"
     gene            1812..>3399
                     /gene="fusA"
                     /locus_tag="PLX53_03275"
     CDS             1812..>3399
                     /gene="fusA"
                     /locus_tag="PLX53_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOY72131.1"
                     /translation="MKTYSTDQIRNIALIGNTGSGKTSLAESMMFEGKVIERKGSIEG
                     KNTISDYTEIEQLNQRSIFSTVLYTEYMDHKLNVIDTPGSDDFVGSVITSMYPTDMVV
                     VTINAQYGVEVGTEIFNRHAERLERPILIAVNQLDHEKANFEQAMDSLRQTYGTKVLQ
                     TQYPLNTGSLFDSFIDVITMKMYKFKDDTGIREEFPIPAEEMEKATALHNTLIESAAE
                     NDETLMEKFFEQGSLSEEEMRKGLSIGVRNRQIFPVFCLSAKKNIGTKRLMEFIIKIG
                     PSPASTPMLKNNSKTEIKCDAAAPTSLFIFKSTVEPHLGEINYFRVMSGKIAEGMDLT
                     NANNSTKERLTQIFAVAGKNRTKVSEMVAGDIGATVKLKNSKTNQTLCGPGIDFTFEP
                     MQFPNPKFRTAIKPKNEAEVEKLGELLNRAHQEDPTIEVEHSKELKQIIISGQGEHHL
                     NILKWQLTNLNKLEVEFIPPRIPYRETITKIAQADYRHKKQSGGAGQFGEVHLVIEPH
                     VEGAPDPVKYKINGKEINLSV"
BASE COUNT          962 a          789 c          725 g          923 t
ORIGIN      
        1 cgatgccgat cggcttgccg ccacaccgtc tcgctttggg gttcaacacc gccaactgca
       61 cagaaagtgg caattgttcc gtcgagaacc cgaagcgagc gttccacctc gacagtaaaa
      121 tcaacgtggc caggagtatc gatgatgttt acttggtact gctggtcgtt gtatctccaa
      181 aatgtagtag tggcagccga ggtaatggta attccacgct cctgctcctg caccatccaa
      241 tccattgtgg cggctccatc gtgcacctct ccaattttat gggttttccc ggtaaaaaag
      301 agaatgcgct cggtagtagt tgttttaccg gcatcaatgt gcgccattat tcctatgttg
      361 cgagtatatt ttaaatccct gctcatctat attcctacct tcctcgtgct gtggtattgt
      421 taaaaacgga aatgggcaaa agctttgttg gcctcagcca ttttatgcat atcctctttc
      481 ctcttaaatg cagctccttc ctcgttgtag gccgccataa tttcggcagc aagcttttcg
      541 ctcattccct taccgctacg tttacgggca taaagaatga ggtttttcat ggaaagcgat
      601 atttttcgct cgggacgaac ctccatagga acctggaaag ttgccccccc taccctgcga
      661 cttttcactt caacctgtgg ggtgatgttc tccaaggctt tcttccatat ttcaagcggg
      721 ttcttatcag cgtctttcat ccgttgtcct actatgtcga gcgattggta gaagatgtcg
      781 aatgcaacgc ttttcttgcc gcgctccatc aggttgttaa caaacctggt aaccatcacg
      841 tcgccaaatt taggatcggg cagtattaat cttttttttg gtttagattt tctcatcgct
      901 gtatataatc aaatgaggtt aacattactt actttttctt aggccgcttg gcgccgtact
      961 ttgagcgtcg ctgtttgcgc ccttcaactc ctgaagcatc gagtgttccg cgtatcagat
     1021 ggtaacgaac tccaggtaaa tcttttaccc tgccaccacg aatgagcaca atggagtgct
     1081 cttgcaggtt gtggccttct ccaggaatgt aggcgttaac ctccttgccg tttgtaagcc
     1141 taaccctggc aacttttcgc atagccgaat ttggcttttt aggagtagtg gtgtacacac
     1201 gtacacatac cccgcgtctc tggggacaag catccagcgc aggcgccttg ctcttatcca
     1261 ccattttaga ccttcctttt cttactagct gctgaattgt gggcataaat acagtttatg
     1321 ttaaattggt tttacaaaat aggtgcgcaa aggtattgat tttttcaata ccatcgttca
     1381 acttgttatt tttttgctgt tattcagcaa gtttacctgc cgacaactaa ttttgcgggt
     1441 gcaaagatat acttttttaa cataaaaagc catcggcact cttcaacctt gaaagcagca
     1501 gctaataaaa ttggccagga ttagtagcat taccctaata atcagctgct taatttgaat
     1561 aaaaaaaaga atgctcaggg ttgattttta tactgtatcc ctggataaag aggcaaggca
     1621 accatttttt tatagaatca cagaacaaac ttgcatattt caaaatttta ctatttttgg
     1681 gggcattcaa caatcgccaa aggctactag agccgtattt cttacattac ctttataaaa
     1741 taaataatag taggtgctgg gattgagacg atagcagaaa agaatgtcaa acactaaata
     1801 gcatattacg catgaaaact tactcaacag atcaaattag aaatatagcc cttattggaa
     1861 ataccggttc ggggaagacg tctctagccg agtccatgat gtttgaaggg aaagttattg
     1921 agcggaaagg ttctattgaa gggaagaata ccatttcgga ctacaccgaa attgagcagc
     1981 tcaaccaaag gtcaattttt tcaacagtgc tgtacaccga gtacatggac cataagctca
     2041 acgtaattga tacaccgggg tcggacgatt ttgttggaag cgttatcacc tcaatgtatc
     2101 caaccgatat ggtggtggta accatcaacg cccagtacgg agtggaagtt ggtaccgaga
     2161 tattcaaccg tcatgccgaa aggttggaaa ggcctatcct tatagcagtt aaccagctcg
     2221 accacgaaaa ggccaacttt gagcaagcaa tggattcgct taggcaaacc tatgggacta
     2281 aagtactgca aacccagtat ccgctaaaca ccgggagcct atttgacagc ttcattgacg
     2341 taatcaccat gaagatgtat aagtttaagg atgatacagg cattcgcgaa gagttcccga
     2401 taccagccga ggaaatggaa aaagctaccg cccttcacaa taccctcata gaatcggctg
     2461 ccgaaaatga cgagacttta atggaaaaat ttttcgagca aggttccctt tccgaagagg
     2521 aaatgcgcaa gggtctatct ataggagttc gcaaccgtca aattttcccc gttttctgcc
     2581 tgtcggccaa aaagaacatc gggacaaaac gactcatgga gtttatcatt aagattggcc
     2641 catccccggc ttctacaccc atgcttaaga acaacagcaa aacggaaatt aaatgcgatg
     2701 ctgctgcgcc aacatctcta ttcatcttca aatctactgt tgaaccgcat ctaggtgaaa
     2761 tcaactactt ccgggttatg tctggtaaga ttgctgaggg aatggacctg accaatgcta
     2821 acaactccac caaggaacgg ttgacccaga tttttgccgt agcaggaaaa aatcggacaa
     2881 aggtatcgga aatggtggct ggcgatattg gggctaccgt taagctaaag aattccaaaa
     2941 ctaaccaaac gctctgcggg cctgggattg atttcacatt tgaacccatg caattcccca
     3001 atccaaaatt ccgaaccgca ataaaaccaa agaatgaagc cgaggttgag aaacttggag
     3061 agctgcttaa ccgtgcgcac caggaagatc cgaccattga agtggaacat tcaaaggagc
     3121 ttaagcagat aataatttcg ggacagggtg agcatcacct caacatcctt aaatggcagc
     3181 ttactaacct caacaagcta gaagttgagt ttatccctcc gcgaataccc taccgtgaaa
     3241 ccatcaccaa aattgctcaa gccgactacc gccataaaaa acaatcggga ggtgcaggtc
     3301 agtttggtga ggttcacctg gtaattgaac cccatgtgga gggagccccc gatcctgtta
     3361 agtacaaaat aaatggcaag gaaattaacc tttccgtta
//
LOCUS       DAOOQX010000145         3395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_33395_length_3395_cov_3.002994, whole genome shotgun sequence.
ACCESSION   DAOOQX010000145 DAOOQX010000000
VERSION     DAOOQX010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33395_length_3395_cov_3.002994"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PLX53_03280"
     CDS             complement(<1..457)
                     /locus_tag="PLX53_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOY72132.1"
                     /translation="MVIDSLSGFKVNSSLITSEYAFYINNAQKVEISFVEGDANTPTA
                     TMRIGYNEFINDLRIGYTNNQSDYAVFNINFSEFEKIPGLTLCECRNFQIGKQVATLG
                     FNFGENNLSIKSAVISSAFSSKNGIRCLELDGLTCFGNSGSPVIDPDSMQ"
     gene            complement(555..683)
                     /locus_tag="PLX53_03285"
     CDS             complement(555..683)
                     /locus_tag="PLX53_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72133.1"
                     /translation="MKKENSIREKISLKFKDDAGVFTIFFLVTLLFVYALIKALTL"
     gene            940..>3395
                     /locus_tag="PLX53_03290"
     CDS             940..>3395
                     /locus_tag="PLX53_03290"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HOY72134.1"
                     /translation="MVELLTQEVESRFGRKISCQKDCISLSISIMDTCREYLSPATLR
                     RLFGLLATNSNPSHVTLDILSRYAGYTGWDDFVSHKKSKIPEGIIRSADEDWEQAAEH
                     AKAVSSKTLNHIMHLSGIRFEETVHRRQVEERLDQFLNSNNPATAIIAPGGYGKSILL
                     AHWYQIHRDLKANMNDVFLLAPATMLDHYASSNTFIGHWLAQLLGQKSLESLLVKDLP
                     GRIVFIFDALDEVVSVGAKFNKILEALADLAEAFAQTNRFKLVISTRLSTWTQLKPYI
                     THPLLWYFSDSELLTHEGANIPPLSPDEVQQILDQTINLRFNQRLLIFELHPDLRQTL
                     SYPYFLQLFIQVYTPEKQNLLNNQHEILLEFLKSKIYFSTLPDEKVDILTEIIRLGNG
                     HRVLKEDIKRSYPIHLKLSGNYFTAYHELLSYGIITEEQVVESTGRYFKYVRITSPQL
                     TGLLIVQDIVNSTGGITEETFRQVEEKFAGTELYPLVQGTLYYTGSRDRNEKVLVKFF
                     ELCPNAYTHGQLLREICATLRNSSYLLNVLMPEYTKTRLGRQLLVEQNIDLNHLNGYF
                     TTVLKHYLSNTTDISELFFAQTLLAFNGFITLNGMLAQPNHEATRNVKPPADVSPLVA
                     GAWFSNQLFAEIIMGNGNNTTPIVERATSYYRKFTSYAERAEFSELIVPTLLLLGQHP
                     AVENYLEDISSIAGSHYQVVNYFNRKLFEILSGKNHFSAEEDCQIQQIYQQLNPIRSF
                     PQVIAGEVGRAASYFHTQNLTMAHQCIRNAIELSGIAGYKLVEMKLMNGLARVLSEMR
                     EEKQAMLCREYAETLWKCSG"
BASE COUNT         1001 a          808 c          669 g          917 t
ORIGIN      
        1 cctgcatgct atcggggtca ataaccggcg aaccgctatt tccaaagcaa gtaagcccat
       61 ccagttcaag acatctaatc ccatttttac ttgaaaatgc cgaagaaata acagcggatt
      121 ttatcgacag gttgttttcg ccaaagttga aacccaatgt agccacttgt tttccaattt
      181 gaaaatttct acactcgcac agggttaaac cgggtatttt ttcaaattca gaaaaattta
      241 tgttgaaaac tgcgtaatcg gactggttat tggtgtagcc aattcgtaaa tcgttaataa
      301 attcgttgta gccaatcctc atggttgccg ttggagtatt ggcatcgcct tcaacaaagg
      361 aaatctccac cttttgggca ttgtttatgt aaaatgcgta ctcgctggtt atcaacgaac
      421 tgttaacctt gaatccggaa agagaatcaa taaccattcc cctttcatta atgaagtgaa
      481 ggctacaaac tgatgcgtgg cattgttccc atacttgtga tagcataacc gtaagttttt
      541 ttagtctgtt aacatcaaag tgttagcgcc ttaataagcg catatacaaa aagcagggtt
      601 accaaaaaaa agatggtaaa caccccggca tcatccttaa acttcagcga tattttctcc
      661 cttatggaat tttccttctt cataacttat ctttgcttaa acactaaccc tggtttactc
      721 cccgcaagta catttcattt tgattggttt gcttaccccc acttattaca tatgaaatgt
      781 ttgccagttc aaatttatcc catttcagat taccaggaaa agtgtcacag ctgtcaagtt
      841 ttagaaattt ccagatttgc tatctaaaca atcacatatc tttgcatagt taacggtatt
      901 tatacatttc agttagctta ataaacatag tatcatacca tggttgagct gctgactcag
      961 gaggttgaaa gtaggtttgg caggaaaata agctgccaga aagactgtat ctccctctct
     1021 atcagtataa tggatacatg tagggagtat ctgagcccgg caaccctacg aaggctgttc
     1081 ggtcttttag ctacgaattc taatccatcg catgtaaccc ttgatattct ctcgcgatat
     1141 gcaggatata cgggttggga tgattttgtc agtcacaaaa agagcaaaat tcctgaagga
     1201 atcattcggt cagctgatga agattgggag caagccgctg aacatgccaa agcggtcagc
     1261 agtaaaacac tcaaccatat catgcattta tcaggcatta ggtttgaaga gacagtccac
     1321 cgacggcagg tggaagagcg ccttgaccaa tttttgaaca gcaacaaccc tgctactgct
     1381 attatagctc ccggaggcta cggtaaatca atcctgctcg cccattggta ccagatacac
     1441 agagatttaa aggctaacat gaacgacgta ttcctactgg caccggcgac catgctcgac
     1501 cattacgcaa gctccaacac ttttatcggc cattggctgg cacagcttct tgggcaaaaa
     1561 tcgttagaaa gcctacttgt aaaagatctt cctggtagaa tagtcttcat ctttgatgct
     1621 ctggacgagg tagtgtcggt aggagccaag ttcaacaaga tactcgaagc tcttgccgac
     1681 ctggctgagg ctttcgccca aacaaacagg tttaagctgg ttatttcgac ccgactgtca
     1741 acttggactc agctaaagcc atacattacg cacccactgc tttggtattt ctccgattct
     1801 gagctgctaa cccacgaagg agccaatatt cctccgcttt cgcccgacga agttcagcaa
     1861 atccttgacc aaaccataaa cctaagattc aaccagcgtt tgcttatttt tgaacttcat
     1921 cccgatttaa ggcaaacgct tagctatcct tactttcttc agctgtttat ccaggtttac
     1981 acccctgaga agcagaattt gctcaataac cagcacgaaa tactattaga gtttcttaag
     2041 agtaaaattt acttttctac actgcccgac gaaaaagttg atatcctaac ggaaataatc
     2101 aggctgggca atggccatcg tgttttgaag gaagacatta agcgaagcta ccctatacat
     2161 cttaaacttt cgggtaacta ctttacagca taccatgaac tcctgtccta cggcattatc
     2221 accgaagaac aggttgttga atcaaccggc cggtatttta agtacgttcg catcacaagc
     2281 cctcaactaa cagggctgct tattgtccag gatattgtta actcaaccgg aggcatcact
     2341 gaagaaacct ttcggcaggt agaagaaaaa tttgcaggta ccgagctcta ccctctcgtg
     2401 cagggaactc tctactacac cggcagcaga gaccgaaacg aaaaagttct cgtaaaattt
     2461 tttgaacttt gtccaaacgc ctacacccat ggccagctat taagggagat ctgcgctacg
     2521 ctcaggaata gcagctacct attgaacgtc ctcatgcccg aatataccaa aacacgatta
     2581 gggcggcagc tcctggtgga gcaaaacatt gacctaaacc accttaacgg ttattttacc
     2641 actgtgctta agcactattt gtccaacact accgacattt ccgaactctt ttttgcccaa
     2701 actcttcttg cattcaatgg gttcataacc ctaaacggaa tgctagcaca acctaaccac
     2761 gaggcaacac gtaatgttaa gccacccgca gatgtttcac cgctagtggc aggagcgtgg
     2821 ttttcaaacc agctctttgc cgaaataatc atgggcaatg gcaataatac caccccgata
     2881 gttgaacgcg ccacttcata ctacagaaaa tttacctctt atgccgagcg ggcagaattt
     2941 tcggaactta ttgttccaac cctgcttctc ctagggcaac acccggcagt tgagaactat
     3001 cttgaagaca tcagctccat tgcaggatcg cattaccagg ttgttaacta cttcaacaga
     3061 aaactctttg aaatactcag cggtaaaaac cacttctcag ccgaagaaga ttgccaaatt
     3121 cagcaaatat accaacagct caaccctatt cgaagcttcc cgcaagtaat tgcaggcgaa
     3181 gttggtaggg ctgccagcta ctttcatacc caaaacctta ctatggccca tcagtgcatc
     3241 aggaatgcca ttgagctgag cgggattgca ggctacaaac ttgttgaaat gaagctgatg
     3301 aatggcttag cccgagtgct ttcggaaatg agagaggaaa aacaggcgat gttatgcagg
     3361 gaatacgctg aaacactctg gaaatgctca gggtt
//
LOCUS       DAOOQX010000146         3393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_33415_length_3393_cov_3.162073, whole genome shotgun sequence.
ACCESSION   DAOOQX010000146 DAOOQX010000000
VERSION     DAOOQX010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33415_length_3393_cov_3.162073"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..177)
                     /locus_tag="PLX53_03295"
     CDS             complement(31..177)
                     /locus_tag="PLX53_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72135.1"
                     /translation="MKKLPPQNEGKDKGFQIPELVRLPAELVRLLAELVRLPADSTLR
                     QVLQ"
     gene            214..678
                     /locus_tag="PLX53_03300"
     CDS             214..678
                     /locus_tag="PLX53_03300"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HOY72136.1"
                     /translation="MIQRIQSLYLLISAILLAFFFFMPYAHYAVGPQEVKISLSASGL
                     GQDGSVVENLPKLWAVLALVILAFTATLVTIFLFKKRKLQARLCIFNIVLLIGLQGLL
                     FYIAKAVEQRLMAPPSYGLIFIFPLVCAIFTYLAYRAIAKDEALIRSLDRLR"
     gene            complement(670..1863)
                     /locus_tag="PLX53_03305"
     CDS             complement(670..1863)
                     /locus_tag="PLX53_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY72137.1"
                     /translation="MKKFLTRTIVMLSLVSLFTDIASEMLYPIMPVFLKSIGFSVLLI
                     GILEGLAEATAGISKGYFGHLSDRLGTRVPFIRWGYTLSAISKPLMAVFTWPIWIFGA
                     RTLDRLGKGIRTSARDALLSDESTRETKGKVFGFHRGMDTLGAAIGPIAALIYLHFYP
                     QNYSTLFLIAFAPGLVAVSLTLLLKDKKKPETSNQQVNPVQGKGFFSFVKYWGHSPKA
                     FRYLVVGLLFFTLFNSSDAFLLLMVKEKGFSDANMISLYIFYNLLYALLAYPMGWLAD
                     KFGMKRIMIFGVSVFGLVYLLFGFADKLHMLLLLFGLYAFYAAATEGVSKAWITNIVP
                     GESTATAIGFYTGFQSVATLLASSMAGLIWTKFGASTVFVISGLAAFITAIYLMLATR
                     MNKLA"
     gene            2336..>3393
                     /locus_tag="PLX53_03310"
     CDS             2336..>3393
                     /locus_tag="PLX53_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOY72138.1"
                     /translation="MKKTRFYLCALGILLGFSAGAQDQVNWNTPIPLDRNVRFGQLEN
                     GLTYYIRKNTEPQQRAEFFIAQNVGAILEEDNQNGLAHFLEHMAFNGTKHFPGKGIIN
                     YFETVGVKFGFNINAFTSLDETVYYLSDVPTNREGIIDSALLVLHDWSGFISLEPDEI
                     EAERGVIREEWRTGRNAERRMYKQVMPVIYKGSKYAERDVIGDINVINNFKHEEIKNY
                     YHKWYRPDLQSIVIVGDVDVDQVEQKIKTLFADIPKRENPDVRPFFELPDNDEPLIGI
                     ASDPEARNTILTVYYKHQAVPKNEKNLGYVREQMIQKLATSMISARLNEITQKPNPPF
                     VFAASDMGNMVRTKSALM"
BASE COUNT          983 a          782 c          759 g          869 t
ORIGIN      
        1 acaaatgtta tagtgagcca gccgaactac tcactgtaac acctgtcgaa gggtggaatc
       61 cgccggtagg cggacaagtt ccgccagtag tcggacaagt tccgccggta ggcggacaag
      121 ttctggaatt tgaaatcctt tatcctttcc ttcgttctga ggaggtaatt ttttcataca
      181 tttgtctaaa actactttta agcatattca gccatgatac agagaataca atcgctttac
      241 ctcctaatat ctgcaatttt actggcattt ttctttttca tgccatacgc ccattacgct
      301 gttgggccac aggaggttaa aatttcgctt agcgcatcgg gtttaggtca ggatggttca
      361 gtagtggaga acctacctaa actttgggca gtccttgctt tggtcatact ggcttttacc
      421 gctaccctgg taaccatatt cctctttaaa aaaagaaaac tgcaggcaag gttgtgcatt
      481 ttcaacatcg tgcttctgat tggcttgcag ggcttgctat tttatattgc taaagctgtg
      541 gagcaaaggc taatggcacc cccatcgtat ggcttgatat tcattttccc cttggtttgc
      601 gccattttca cctaccttgc ctaccgggca attgctaagg acgaagccct tatacgttcg
      661 cttgaccggc tacgctaact tattcattct ggttgccagc atcaggtaga tggcggttat
      721 gaatgccgct aagcccgaaa taacgaaaac agttgatgct ccaaacttag tccagattaa
      781 cccggccatg ctgctggcta aaagagttgc cacgctctgg aatccggtgt agaatcctat
      841 agcggttgcc gtgctttcgc caggcacaat gttagttatc caagcctttg aaacgccttc
      901 ggttgcggct gcatagaatg catataatcc aaagagcagc agtaacatgt gtagcttgtc
      961 tgcaaatcca aagagcaggt aaaccaggcc gaacaccgat acgccaaaga tcattatccg
     1021 cttcatgcca aacttatcgg ccagccaacc catggggtag gcaagcagcg catagagcag
     1081 gttgtagaat atgtagagcg aaatcatgtt ggcatcagag aaccctttct ccttaaccat
     1141 gaggagcaga aaggcatcgg agctgttgaa aagggtaaaa aacagcaggc ctaccaccag
     1201 gtagcgaaaa gctttgggag aatgccccca gtactttaca aaagaaaaaa atcccttacc
     1261 ctgaacagga ttaacctgtt ggttacttgt ttctggtttc ttcttatcct ttagtaagag
     1321 ggtaagcgaa actgccacca gacctggagc aaaggctatc aggaaaagcg tgctgtagtt
     1381 ttgggggtag aagtgcaggt atataagcgc agcaataggc ccaatagctg ctccaagagt
     1441 atccatgccc cgatgaaacc caaatacctt cccctttgtt tccctggtgg actcgtcgct
     1501 gagcagggcg tcgcgggcgc tggttcgtat gccctttcca agcctgtcga gtgttcgagc
     1561 accaaatatc caaatcggcc aggtgaaaac ggccatcagg ggcttggaga tggcgctaag
     1621 ggtgtagccc caccttataa agggtactcg cgtccccaac ctgtcggaaa gatgcccgaa
     1681 gtagcccttg cttattccag ctgtggcctc agccaaccct tcaagaatac caatcagcaa
     1741 aaccgaaaac ccaatggact tgagaaaaac cggcattatt gggtaaagca tttcgctggc
     1801 tatatccgta aacaggctta ccagcgaaag catcacgatg gtacgggtta gaaacttttt
     1861 catgcagagt aggttaaatt aaatatccaa agaataatta gcagctagtt aaacctcaga
     1921 acttactatg aataaaaaaa taaagaacaa taattcaatt atttaaccat gttgccatcc
     1981 tttccgaaca gcccggaaag ttattcaaac aaatgttgcc ttgatattca gctgcagaaa
     2041 ttttaattgt tggcatagga aacaggcatt tgaacaggtt ggcaaataca agataaaagt
     2101 ccattggaaa gcataaggat aatcctgatt gttgggattt tgcatatata aaccaggcca
     2161 gagaaaccgg gcaaaatgaa cactttagaa aaatacttat tgagccaggg cttaatccag
     2221 ctataaagtt tcaaaatatg ttaaaaagtg aacgtttagt ttatgtaaca taaaatttgg
     2281 ctacctttga tataaatttt tactaataac aaaaaattaa cttaaacatc gggtaatgaa
     2341 aaaaacacga ttttatctat gcgccctggg tattctacta ggttttagcg ctggggctca
     2401 ggatcaggtg aattggaaca cccccattcc tcttgacagg aatgttcggt ttggccagct
     2461 ggagaacggc ctaacctact acattaggaa gaacaccgaa cctcaacagc gcgcagaatt
     2521 ttttattgcc cagaatgtag gtgcaatact tgaggaggat aaccagaacg gtctggcaca
     2581 ttttctggag cacatggctt tcaacggcac caagcatttt ccgggtaagg gtataatcaa
     2641 ctactttgaa acggttggcg ttaagtttgg cttcaacatc aacgccttta cctcgcttga
     2701 cgaaaccgtt tactacctgt ccgatgttcc taccaaccgc gagggaataa ttgacagcgc
     2761 ccttctggtg ctgcacgact ggtcaggatt catctcgctt gaacccgatg aaattgaagc
     2821 tgaacgtgga gttatccgcg aggagtggcg cactggacgt aatgccgaac gaaggatgta
     2881 taaacaggtg atgccggtga tttacaaggg atcgaaatac gccgagcgcg atgttattgg
     2941 cgatatcaac gtcatcaata actttaagca tgaggaaatt aaaaactact accacaaatg
     3001 gtaccgtccc gatttacaat ccatcgtcat tgttggcgat gttgatgttg accaggttga
     3061 gcagaaaatc aaaacgctgt ttgccgacat acccaaacgg gagaatccgg acgttagacc
     3121 tttcttcgag ttacccgata acgacgagcc gttaatcggc attgcgtccg accccgaagc
     3181 ccgcaacacc atcctcaccg tttactacaa gcatcaggca gttcccaaaa acgaaaaaaa
     3241 tcttggctac gttcgggagc aaatgatcca aaagctggca acttccatga tttctgcccg
     3301 gctgaatgaa attacccaga aacctaaccc tccctttgtt tttgccgctt ccgatatggg
     3361 gaacatggtt cggaccaaaa gcgccctgat gtt
//
LOCUS       DAOOQX010000147         3392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_33428_length_3392_cov_3.217261, whole genome shotgun sequence.
ACCESSION   DAOOQX010000147 DAOOQX010000000
VERSION     DAOOQX010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33428_length_3392_cov_3.217261"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLX53_03315"
     CDS             <1..409
                     /locus_tag="PLX53_03315"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOY72139.1"
                     /translation="ILREGSDIAILTIGHVGNLAATAAGKLEAEGISVFHADMRFVKP
                     LDADLLRSVAKRFTTLVTVEDGCLPGGFGSGVTEWLNDNGYNVRVVRLGIPDEFIEQG
                     PPQQLYAECGFDTENIYQTAKKYVQKKVKMRVG"
     gene            complement(713..1084)
                     /locus_tag="PLX53_03320"
     CDS             complement(713..1084)
                     /locus_tag="PLX53_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72140.1"
                     /translation="MKNKIYLLPLSLLAVFTVVTWSCQKDDDEDVCKKHEIQQCEIVN
                     TCCPTDGGNCYYEYGDQKFYCDQSQATADDPDGCNGAIDDVIAVICPTAKAADIQLAK
                     VQLRELTRKLMNEARMLSVCH"
     gene            complement(1155..1829)
                     /locus_tag="PLX53_03325"
     CDS             complement(1155..1829)
                     /locus_tag="PLX53_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOY72141.1"
                     /translation="MKLKVKLDRHHLIAYAISLVLVVLIGYKLWRYFTPDSKEAVLQE
                     SPEPRPLDIVFGSDTAPLPVYMYASYSCTFCRQFFTDVFPMLKKEFLDNGRVKLVMRL
                     LENTNHPDALNAAKTAVCINRYGYFDKLHELLLTDSRVTYTPEFRDMVNDFTARDELV
                     AECILGGQADEYILQIRNEFRKFGLSGTPTFIINRNVYRGYRSYQEFRKIILHHMETE
                     DRKDIN"
     gene            complement(1813..>3392)
                     /locus_tag="PLX53_03330"
     CDS             complement(1813..>3392)
                     /locus_tag="PLX53_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOY72142.1"
                     /translation="RLIGDIANTSKFGVDVDSLLIKVGNSSLGGRFKVMNLDKPTYSA
                     KSFLNVNFNELRPFIPDTLVETLTGRMAATVQSHGTLNLDSLSSQIMPIAFEQSRFTL
                     NLDSLNFAMPNDTLMRVDNLSLKLAMSDDTIRINGFHGQAMGIEFAIDSTRIWNVYKA
                     MLKGEKDKKLVADTRIQLSDIDFAMFAPFMATDSTAAEPTPQPAEPQENVAGSEAPAT
                     ESSFTIPSYIIRGTVAVNSFKYGNMLIDKISTKFRVDDSLYVIDDLKLNAFGGDLTLS
                     ALYDTRNDTATVIEAKCNLNRMNIRQLLADNNDFDQTFFTHENIEGLATGELFARVIM
                     RDTNILYDKINLLGNFKLENGGIYNFEPLMQLSKFTNLNELERVVFRTLETSVFVYNN
                     EIYFPKTDIVSSAMDISVYGMQSFADDYEYHLIVHLGDVLVGKSNKLLKQQGMESDVF
                     EGQDKANRSGLYLVALNRGKDSKYGFDTQHLQRLMKTTIRVQERGLNLIFNPKVVNFS
                     TEIDRRERKKKPNEAKG"
BASE COUNT          871 a          795 c          810 g          916 t
ORIGIN      
        1 tattcttcgg gagggttccg acatcgccat cctgactatt ggccatgtgg gtaatcttgc
       61 tgctactgca gccgggaagc ttgaagccga aggcataagc gtttttcatg ccgacatgcg
      121 ttttgttaaa cccctggatg ccgacctgct acgctccgtt gccaagcggt ttacgactct
      181 cgtaaccgtt gaggatggtt gtttacctgg tggttttgga agcggtgtaa cggaatggct
      241 taacgataac ggctacaacg ttagggtggt taggcttgga atccccgacg agttcattga
      301 gcagggtcct ccccagcagc tatacgccga atgcggcttt gatactgaga atatttacca
      361 aactgccaag aaatacgttc agaaaaaggt taaaatgcgg gtagggtaaa attttacatc
      421 gagctggcac tcgcaatgaa tttctctgaa agcctttgtg acgcttaaaa tacatactac
      481 acaattaaga ctaactacac gctgtaatta tagcatggcg ttgaggcagt aagcctatta
      541 aatggccttt aattcaaact aacgttgaaa aaggcattaa aaacacaact ttggtgggta
      601 tgagttgccc ttaaatgttg aagtatttaa ccttacaaaa acaagtttgc gcccggcaga
      661 aaatattgca aacacaggcg caaacaaata caacaacggg aacccatcag ggttaatggc
      721 aaaccgaaag catgcgggcc tcattcatca gcttgcgggt tagctcccgt agctgcacct
      781 tggccagctg gatgtcagca gcctttgcag taggacatat taccgcaata acatcatcta
      841 ttgctccatt gcaaccatcg gggtcatcgg ctgtagcctg gctttggtcg cagtagaact
      901 tttgatcgcc atactcgtag tagcagttgc ccccatcggt agggcagcaa gtattgacaa
      961 tttcgcactg ttgaatctca tgcttcttac aaacatcctc gtcatcatcc ttctggcagc
     1021 tccaggttac aactgtaaaa acagccagca acgacaacgg cagcaaatag attttgtttt
     1081 tcatttccat aaattttttg tttgttaaac actgtaggtt tgcttagatt tccatgttta
     1141 caagtggcta tcatttaatt tatgtccttc ctatcctccg tttccatgtg atgtagtata
     1201 atcttcctga actcctggta gctgcggtaa ccccggtaaa cattccggtt gatgataaag
     1261 gtgggtgtac cgctgaggcc aaatttacgg aattcgttcc taatttgcaa tatgtactcg
     1321 tcggcctgtc cgcccagaat gcactcggcc acaagctcgt cccttgctgt aaagtcgttg
     1381 accatgtcgc ggaattcggg ggtgtatgta accctgctat cggtaaggag caactcgtgc
     1441 agcttgtcga agtagccata ccggttgatg cagacagcgg tttttgcggc attcaacgcg
     1501 tcaggatggt tggtattctc cagcagcctc ataaccagct taaccctgcc gttgtctaaa
     1561 aattcctttt tcagcatggg aaaaacatcg gtaaaaaact gcctgcaaaa ggtgcagcta
     1621 tagcttgcat acatgtaaac gggaaggggg gctgtatcgc tgccaaacac gatatccaga
     1681 ggccttggtt cgggcgactc ctgaagcaca gcctctttgg aatcgggggt aaaatatcgc
     1741 cacagcttgt acccaataag cacaactaaa acaagcgata tggcgtaggc aatcaggtga
     1801 tgcctgtcca gcttaacctt tagcttcatt tggcttcttc ttgcgttctc tcctgtctat
     1861 ctccgtgcta aagttaacca ccttagggtt gaatataagg ttaagcccac gctcctgaac
     1921 cctgatggtg gttttcatca accgctgcaa atgctgcgta tcaaaaccat acttggaatc
     1981 cttcccccgg ttaagggcca ccaggtaaag ccccgaacga tttgctttgt cctgaccttc
     2041 aaacacgtcg ctctccattc cctgctgctt gagcagcttg ttggatttac caacaaggac
     2101 gtcaccaaga tgaacaatga ggtggtactc ataatcatct gcaaaactct gcattccata
     2161 aaccgaaatg tccatggctg agcttacgat gtcggttttt gggaagtaaa tctcgttgtt
     2221 gtaaacgaat acgctggtct cgagcgttct gaaaacaacc ctctccaact cgttcaggtt
     2281 ggtgaatttc gacagctgca tgaggggctc gaagttgtaa atacccccat tctccagctt
     2341 gaagttgccc agcaggttta tcttgtcgta caggatgttg gtatcgcgca tgataacccg
     2401 ggcaaacaac tcaccggtgg caaggccttc gatgttctca tgggtaaaga aggtttggtc
     2461 aaagtcgttg ttatcggcaa gaagctgcct gatattcatt ctgttgaggt tgcacttggc
     2521 ctcaattacg gtggccgtat cgttacgggt atcgtagaga gccgagaggg taagatcccc
     2581 gccaaatgcg ttcagcttca ggtcgtcaat aacgtaaagg ctgtcgtcaa ccctgaattt
     2641 ggttgatatc ttgtcaatga gcatgttgcc gtacttaaaa ctgttgacag ccaccgtgcc
     2701 cctgattatg tatgatggaa tggtgaagct gctttcggtt gccggcgctt ctgaccctgc
     2761 aacattttcc tgaggttcag caggttgagg tgttggttca gcagcagtgg aatcggtagc
     2821 catgaatggg gcaaacatgg caaaatcaat atcgctgagc tgtattcggg tatcggctac
     2881 caacttttta tccttctcac ccttcagcat ggccttgtaa acattccaga ttcgcgtgga
     2941 atcaatggca aactcaatgc ccatagcctg gccatgaaat ccattaatgc ggatggtatc
     3001 gtcgctcatg gcaagcttca gcgagaggtt gtcaaccctc atcagggtgt cgttgggcat
     3061 ggcaaagttc aagctgtcga ggttaagcgt aaagcggctc tgctcaaaag caatgggcat
     3121 aatttgactg gaaagcgagt cgaggttgag ggtaccatgc gactgcacgg ttgcagccat
     3181 gcgtcctgta agggtttcaa ccagcgtatc gggaataaaa ggcctgagct cgttgaagtt
     3241 aacatttaaa aacgatttag ctgaataggt gggtttgtcc agattcatta ccttgaatct
     3301 acctccaagg gagctgtttc caaccttaat gaggagtgaa tcaacatcaa caccaaactt
     3361 gctggtgtta gcaatatcgc caatgagcct gg
//
LOCUS       DAOOQX010000148         3370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_33699_length_3370_cov_3.591554, whole genome shotgun sequence.
ACCESSION   DAOOQX010000148 DAOOQX010000000
VERSION     DAOOQX010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33699_length_3370_cov_3.591554"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PLX53_03335"
     CDS             complement(<1..884)
                     /locus_tag="PLX53_03335"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY72143.1"
                     /translation="MKKIRFFIAMLFTVVGLWANAQVTTSEINGKVVDEKGMALPFAT
                     VVAIHEPSGTQYVTTTDNAGNYRLTNLRVGGPYKLSISFVGYSTGVYTDINLNLGESY
                     LQNVKLKESTTSLEEVVVSAGMGNPILSSERTGAQTNISRGDINTLPSISRSITDFSK
                     LTPQAQGNSFVGRDGRFNTITVDGAAFNNNFGLSSNPLPGGNAQPISLDAIDQVSVNI
                     APFDVRVSQFTGASINAVTRSGDNSFKGSAYSYIRPKSFTGDKVDGNDVEGANDRNSQ
                     NFGLSFGGPIIKDKLFFF"
     gene            1370..1603
                     /locus_tag="PLX53_03340"
     CDS             1370..1603
                     /locus_tag="PLX53_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72144.1"
                     /translation="MLGVLKYANSSPSYLFATALIASACRPQAPPIGRSLPATLIVPA
                     QPLPCHSAGSHQPTGSIAEVKFRFLTRRLPVVN"
     gene            complement(1844..>3370)
                     /locus_tag="PLX53_03345"
     CDS             complement(1844..>3370)
                     /locus_tag="PLX53_03345"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOY72145.1"
                     /translation="YEATALMNIGVIYKNKKDYKKALEYFSRAQKFEINQGNYYKSAQ
                     CLANIGEVEFALKNYEKSIAYQEKAFDAFEKVKAKNDAALCLMSIGCAYDSLNKSAMA
                     KSYYAKTLSYIDSLNQPNLKMTLAGQIYSLNKKLNNFEEALKYYEIYTMWKDTILARD
                     NQKNLAELLTKYETVQKEKEIELQKERVKRRDIILLSELLLSVLLIGLVGVLVSSYRR
                     KKRDNEILEQSNSLISQQKEEIQTQRDEVVSQRDRIIRQNRLITDSIEYARHIQSTIL
                     PSREQLSAFFPEFFIFYLPRDIVSGDFYWFKLQENAGILAFADCTGHGVPGAFMSILG
                     ITLLDDIVTPKSSISPSQILEELRVNVKNSLKQSGKRVETKDGMDMAICRFNPASTYL
                     EYSGANMPLTIIRNGEVLEFRPTRNPIGYYPKEMPFENHQVELRKHDMLYLFTDGFSD
                     QLGGPNYQKYKRQNLINLLKSIAGLSMTQQENILAASHSDWKGTYEQVDDILVMGIKF
                     "
BASE COUNT          900 a          744 c          675 g         1051 t
ORIGIN      
        1 ataaaaaaga acaacttatc ctttataatt ggtccgccaa aacttaaccc aaaattttgg
       61 ctgttcctgt cgtttgcccc ttctacgtcg tttccgtcaa ccttatcgcc ggtaaatgat
      121 tttgggcgaa tgtaggagta agccgaaccc ttaaaggagt tgtctccact gcgggtaacc
      181 gcatttatgc tggcaccggt aaactgggaa actctaacat caaaaggtgc aatattcaca
      241 gaaacctggt caatggcgtc caaggatatt ggctgtgcat ttcctcccgg aagggggttg
      301 ctgcttaaac caaaattgtt gttgaaggct gcaccatcaa ccgtaatcgt gttaaaacga
      361 ccatctcttc caacaaaaga gttaccctgg gcttggggtg ttaactttga aaagtcggtg
      421 atgctgcggg aaattgaagg cagtgtgttt atgtcacccc tgctgatgtt ggtttgtgca
      481 ccggttcttt ccgaactcaa aataggattc cccattcctg ccgaaacgac aacctcctct
      541 agggatgttg ttgattcttt taactttaca ttttgcaggt acgattcacc caggttaagg
      601 ttaatgtccg tataaactcc cgtggagtac cctacaaacg aaatgcttaa cttgtagggt
      661 ccaccaacac gcaggttggt taaacgatag ttaccagcat tgtcagtagt ggtaacatac
      721 tgcgttcccg aaggctcatg gatggccaca acagtggcaa atggcaatgc cattcctttt
      781 tcatctacta ccttaccgtt tatttccgaa gttgttacct gggcgtttgc ccataagcca
      841 acaacggtaa acaacatggc gataaaaaac ctgatttttt tcattaaatt aaaatttggt
      901 tagtaatggt ttaattattg gcaaataaat taacacataa ggtgcatgta aaacaaaact
      961 ttatataata ggtttaactt ttgctactgc attcgaaata tctggactta cccaaaaatc
     1021 actttccaac atcatactac ttgaatatgt ttcggatttg cagctggtaa tacggatact
     1081 tttttgtcat gccaaagatg agtcaaaata atctaacggc tgatagctat atattaaaaa
     1141 atgattaagt ctaaacggtc aaagggttta aatctctttt ttgaacgaaa ttaaggtttt
     1201 gcccttcttc ttgcctgata ataactgcac taagttataa aaatatgcct tatagtgttt
     1261 tcaatgttaa acttatctca gcgtaattct agagttttgg aataaaaaca agctatttca
     1321 aaataagtgt ttgcattatt tccatataaa tttattataa taggattttt tgcttggtgt
     1381 cttgaaatat gctaacagta gccccagcta cctttttgcc acagcgctga tagcttctgc
     1441 ctgtcgccct caggcacctc ccataggtcg aagtcttcct gcaaccctta tagttccagc
     1501 acaacccctt ccctgtcatt ctgcaggttc tcatcagccc accggtagca ttgcagaggt
     1561 aaagtttcga ttcctcacca gaaggcttcc tgttgtcaac tgaaagggat taaacccaaa
     1621 cctccgacat gttgctagcg acggagctcg gctaccacct cctcgttggg tattcatcca
     1681 cagctatgcg cctctgagaa tgctacacag caattgttga taaatttggc aaatctacta
     1741 gtagcgttaa cactcaacgc ccatctagtg gttctaagta acgggattaa actaccggct
     1801 ttgagatcat atcctaaatt tgaagatacc caggcgactg accctagaat tttattccca
     1861 ttaccagaat gtcgtcaacc tgctcgtagg tacccttcca atcggaatgg ctggcagcca
     1921 aaatgttttc ctgttgggtc atggaaagcc cagcaatgga ttttaaaagg ttgataagat
     1981 tttgcctctt gtacttttgg tagttggggc cgcctagctg atccgaaaag ccatctgtaa
     2041 aaaggtatag catatcgtgt ttgcgtagct ctacctggtg gttttcaaaa ggcatttcct
     2101 ttggatagta tccaatgggg tttcgtgtag gcctgaattc cagcacctcg ccattcctga
     2161 ttattgttaa cggcatgttg gctcctgaat actcaagata tgttgaggct gggttgaacc
     2221 tgcaaatagc catatccata ccatccttgg tttcaacccg tttacccgac tgtttgaggg
     2281 agttttttac gtttaccctt aactcctcaa gaatttggct tggagatatc gaactcttcg
     2341 gggtaacaat atcgtccaga agggttatgc ccaaaatgct cataaacgcc ccgggtacac
     2401 catgccccgt gcaatcagca aatgccagaa tacctgcgtt ttcttgaagc ttaaaccagt
     2461 aaaaatcgcc gctgacaata tctcggggaa gataaaaaat aaaaaattca gggaaaaatg
     2521 ctgaaagttg ctccctggat gggagaatgg tcgactggat gtgccgggca tactcaatgc
     2581 tatcggtaat cagcctgttc tgacggatta ttctatcgcg ctggctaacc acctcatctc
     2641 gttgtgtttg gatttcttcc ttttgctgcg aaatcaagct attactttgt tccagaattt
     2701 cgttgtcgcg ttttttcctg cggtagctcg ataccagcac cccaactaac ccaataagaa
     2761 gaacagaaag cagaagttca gacaagagaa taatatccct cctttttacc ctttcctttt
     2821 gaagttcgat ttccttttcc ttttgaaccg tttcatactt ggtgaggagc tctgccaggt
     2881 ttttctggtt atccctggcc agaatcgtgt ccttccacat ggtgtaaatt tcgtagtact
     2941 ttagggcttc ttcaaaatta tttaactttt tattcaggct ataaatttgt ccggccagcg
     3001 tcattttaag gtttggctgg ttaagagagt caatatagct caaggtttta gcgtagtaac
     3061 tttttgccat tgcgctttta ttcaagctat cgtaagcaca gccgatgctc atcagacaga
     3121 gggctgcatc gttttttgcc ttgacctttt caaaagcatc gaaggccttt tcctggtagg
     3181 ctattgactt ttcgtagttc ttgagggcaa actctacttc tccaatatta gcaaggcatt
     3241 gggccgattt atagtagttt ccctggttga tttcaaattt ttgagccctg ctgaagtatt
     3301 cgagtgcctt tttgtaatcc tttttattct tataaataac tccaatgttc atgagcgctg
     3361 ttgcctcgta
//
LOCUS       DAOOQX010000149         3353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_33950_length_3353_cov_2.428441, whole genome shotgun sequence.
ACCESSION   DAOOQX010000149 DAOOQX010000000
VERSION     DAOOQX010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33950_length_3353_cov_2.428441"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="PLX53_03350"
     CDS             complement(<1..1228)
                     /locus_tag="PLX53_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOY72146.1"
                     /translation="MKKTLLTLVLITWGWVLSAQEITVTGTVISASDGIPLPGVSVTV
                     KANPSIGTATGIDGRYSIKVDANATLLFSFVGMETKEMPVNGQTIINVSMVEATQVLD
                     ELVVVGYGIQKKSLLTSSVAKVGSDEIAKTNPLRVEQALQGKTAGVQIISNSGQPGDG
                     LTVRIRGIGTNGTSDPIYLVDGMPVGGIDYLNPNDIESVQVLKDASATAIYGARGANG
                     VVLITTKSGSKGKMAVTYDYSFGLQNAWRKVSLLDASEYALIMNESYANDNAAIPFAD
                     VASLIDTVGSGTDWLDELFYKNAPVQNHQFSVSGGNDNSSFYSSFSYTTQDGIVAKGK
                     SNYERYTYRINSNHQYGKFGFGNNLSYSNKKNRGIDPNAEFGGPLAKAVNMDPLTPVK
                     YADGTWGVSSYAAQEVV"
     gene            1588..2214
                     /locus_tag="PLX53_03355"
     CDS             1588..2214
                     /locus_tag="PLX53_03355"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOY72147.1"
                     /translation="MRSSRINMTKSKLLPLAILLGITLSSGHSHAQVRDSVVVNLYTI
                     VFYEGDTIPYIKLREVVKYGKRRFKSRSEMNQYYKMIRNLKLTYPYAQLAKYKLLEMN
                     EDLKMIKTERERREYINQAEKELRMQYEKDLRKFTVSQGKMLMKLIDRETGATSYDLV
                     KELKGGFSATFWQGVARLFGSNLKVKFDPEGEDKLLNELIGLYEKGLL"
     gene            complement(2227..2883)
                     /gene="elbB"
                     /locus_tag="PLX53_03360"
     CDS             complement(2227..2883)
                     /gene="elbB"
                     /locus_tag="PLX53_03360"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HOY72148.1"
                     /translation="MANSKKFAVVLSGCGVLDGSEIHEAVMTLYAITLSGSSYEIFAP
                     DVNQHHVVNHLNGSEVDQTRNVLVESARIARGKIKPLGDFSSDVFDAIIFPGGFGVAK
                     NLSSWAFDGAGCSVNADVANAVRSMHRLHKPIGALCIAPVVIARILGGVTVTIGKDAA
                     TVKEIETLGARHQMALQTQVVVDEKNMIFSTPCYMLDATIVDIAEGAKAIVSEMLKWI
                     "
     gene            2968..>3353
                     /gene="thpR"
                     /locus_tag="PLX53_03365"
     CDS             2968..>3353
                     /gene="thpR"
                     /locus_tag="PLX53_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOY72149.1"
                     /translation="MKKERTFIAVGIKPEAKLLNCWHKLKQQHNLDFINWVDEGMLHL
                     TLRFLGEIPVDLVGDVSKVLSISLTGFNSFSLKGKELGSFGRPTPRVIWVGIEQNETL
                     NNLKQTIDKALLPLGFDREKGLFMPH"
BASE COUNT          943 a          771 c          690 g          949 t
ORIGIN      
        1 ttacaacttc ctgggcggca tagctggaca ctccccatgt gccatcggca tattttaccg
       61 gagtaagcgg gtccatatta actgctttgg ccagtggccc gccaaattcg gcgttaggat
      121 caatccctct gtttttctta ttgctgtatg aaaggttgtt gccaaatcca aactttccat
      181 actgatggtt ggagttaatc cggtaggtat aacgttcata gttcgacttt cctttcgcaa
      241 caataccatc ctgggtagta taggaaaagg atgagtagaa cgaagaattg tcgttgccac
      301 cgcttaccga gaactggtgg ttctgcacgg gagcgttttt atagaatagc tcatcgagcc
      361 agtcggttcc actaccaacc gtatcaatca gtgatgctac atcagcaaaa gggattgcag
      421 cattatcgtt ggcataactt tcgttcataa tcaaggcgta ctcgctggcg tcaagtaacg
      481 aaactttacg ccatgcattc tgtaaaccga aagaatagtc gtaggtaacg gccatttttc
      541 cctttgaacc gctctttgtt gttataagca ctactccatt agcacctcgt gcaccgtaaa
      601 ttgccgtagc cgaagcatcc ttcaacacct gaacagattc aatatcgttt ggattaaggt
      661 agtctatccc acccaccggc attccatcaa ccaggtaaat agggtctgac gtaccattag
      721 taccaatacc cctaatacgt acagttaatc catccccagg ctggccggag ttggaaataa
      781 tttgaacccc tgccgttttt ccctgtaaag cttgttcaac tcgcaaggga tttgtcttgg
      841 caatttcatc acttcccacc ttggctaccg aagaggtgag caggctcttc ttttggatgc
      901 catacccaac tacaaccaac tcatcgagaa cctgggtagc ttcaaccatg gaaacattaa
      961 ttattgtttg cccgtttaca ggcatttctt ttgtttccat tccaacaaat gagaacaaaa
     1021 gtgtagcgtt ggcatcaacc ttgatgctgt agcgcccatc aataccggtg gctgttccaa
     1081 ttgaaggatt ggccttaacg gtaaccgaaa cacccggtag tggtattcca tcactagccg
     1141 aaattacagt tcccgtaact gtaatttctt gtgctgacag aacccacccc caagtgatca
     1201 aaactaaagt cagtagtgtt tttttcattg gtttggtttt tggttaacag gaggcaaggt
     1261 attacatcct gctggttcat gtcaaaaaac cacccacatc ataaccacta tataataatt
     1321 tcaaaagttt ctcatctgtc gaaaacgttt tcggagcagt acgtcaaccg tacgtctcta
     1381 agcctgaggt aagatttata atgctgtatt ttaagctatt ataatttaaa tgtattctgt
     1441 aaaatttggt ttaatgccgc cgcaattttt ataatttgag caggcttgtt tttacaaatt
     1501 ctcaactatg gcataaaagt tgatttttgc cgtttcgcat aaggtaaaca ttcggtagaa
     1561 agggttgttt taatgttact tttgcaaatg agaagttcaa ggataaacat gacgaaaagt
     1621 aagctactac cattggctat cctgctggga atcaccttgt catcgggtca ctcgcatgct
     1681 caggttagag attcggttgt ggttaacctt tatactattg ttttctacga gggtgataca
     1741 ataccctaca tcaagttgcg agaggtagta aagtacggca aacggcgctt taaaagccgt
     1801 agcgaaatga accagtacta taaaatgatt agaaatttaa agcttaccta cccctacgcc
     1861 cagcttgcca agtacaagct acttgagatg aacgaggatc taaaaatgat taagaccgaa
     1921 agagaacgca gggaatacat caaccaggcc gaaaaggagc tccggatgca gtatgagaag
     1981 gacctgagaa aattcaccgt ttctcaagga aaaatgctga tgaagctcat cgacagggaa
     2041 accggcgcaa cctcctacga tttagtaaaa gagctgaagg gtggattttc agcaacattt
     2101 tggcagggtg ttgcacgact ttttggttca aaccttaagg ttaaatttga ccctgaaggt
     2161 gaggataagc tgctaaacga gcttataggt ctttatgaaa aaggcttgct ataatatatt
     2221 ctcccgctat atccatttaa gcatttcgga aactatagcc ttggccccct cggcaatatc
     2281 cacaattgtt gcatcgagca tgtagcatgg tgtagaaaaa atcatgtttt tttcatccac
     2341 cacaacctgc gtttgcaggg ccatctgatg tcttgcaccc agggtttcta tctcctttac
     2401 tgttgcagca tcctttccta ttgttactgt aactcctcca agtatcctgg caattacaac
     2461 cggagctata catagcgctc ctattggctt atgcagcctg tgcatagacc gaaccgcatt
     2521 agccacatcg gcattcacgc tgcagcccgc tccatcaaaa gcccacgacg ataaattctt
     2581 ggccacccca aaccctccgg ggaaaataat tgcatcaaac acatcggagc taaagtcgcc
     2641 aagcggttta atctttccac gtgcaattct ggccgattca acaagaacgt tacgggtttg
     2701 gtccacctcg cttccattaa ggtgattcac cacatgatgc tggtttacat caggggcaaa
     2761 aatttcatag ctgctccccg ataaagtgat agcgtaaagc gtcattactg cttcgtgaat
     2821 ctcggagccg tctagtacgc cgcaccccga aagaaccaca gcaaattttt ttgagttggc
     2881 catgcccagt caattttttt tatttttggc tacaatttac aactttttaa aagttttgca
     2941 aggaaagtac ataaatctcc atgaaagttg aaaaaggagc gaaccttcat tgcggttggc
     3001 attaagcctg aagccaaact tttaaactgc tggcacaagc ttaagcagca gcataacctc
     3061 gatttcatta actgggttga cgagggcatg cttcacctca cactccgttt tctgggagaa
     3121 ataccggttg atcttgtagg cgacgtttct aaagtgcttt ctatatcgct cactggcttc
     3181 aactcattca gccttaaagg taaggagctt ggaagttttg ggcgtcctac accccgggtt
     3241 atatgggttg gcattgagca aaatgaaact ctgaacaatc ttaaacaaac aattgacaaa
     3301 gcgcttctcc ccttaggatt tgatagggaa aaaggccttt ttatgcccca cat
//
LOCUS       DAOOQX010000150         3317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_34425_length_3317_cov_3.120785, whole genome shotgun sequence.
ACCESSION   DAOOQX010000150 DAOOQX010000000
VERSION     DAOOQX010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34425_length_3317_cov_3.120785"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLX53_03370"
     CDS             complement(<1..625)
                     /locus_tag="PLX53_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72150.1"
                     /translation="MKVNGVNYRTIWLEDGSSPCVKIIDQRYLPHRFVVEPIESFDGL
                     LRAISEMHLRGAGLIGVAAAFGIYLAVWEQRNLVNFQEALEKCALKLIKTRPTAVNLR
                     WAVERQLEVLAGVENPDDRVAIARQTAIDIAEDDAGCCRKIGLEGVGLIEDIARNKNG
                     GVVNILTHCNAGWLAFTDYGSALAPVYEAWRRGVRLHVWVDETRPRNQ"
     gene            943..2457
                     /locus_tag="PLX53_03375"
     CDS             943..2457
                     /locus_tag="PLX53_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOY72151.1"
                     /translation="MNKRIFILIIIDLMLILVAFYLSVLLKGVSFASYLESYQNALLL
                     FALIWIATSIVFEKYVFTTHSARKISANIIKSNLLAFAVVTFLIYFTRSDRYSRFIVF
                     NTILMVTLLELFIYNLWAGLKRIRVIPDNLLGMIERSVKRRTPPIQTTRAPNPMRVEL
                     IKRTLQAEFGNEVYEYLNTNTRLFDDKTLIVSTTTSFNITNQPDGYFDTLINLKRTND
                     IRRINKFFEAVNQKLPRGGIFVCMAETQEQRKKRILSKFPPVINWIYYFFDYVVKRVF
                     PKFSLTKGIYFFLTRGENRVISRAELLGRLYSCGFYVNNETEIEKLQYVVAIKNRKPF
                     FDEEPTYGPIIKLRRVGKNGKIIRVYKFRTMHPYSEYLQEYIFNLHNLEEGGKFRNDF
                     RVSTLGAVMRRLWIDELPMITNLFRGELKIVGVRPLSEQYFNLYTPELQERRIKYKPG
                     LVPPFYMDNPKTLEEIMASEVKYLDAYDKHPLFTDIRYLFGAAYNIIFKKYRSS"
     gene            complement(2462..2683)
                     /locus_tag="PLX53_03380"
     CDS             complement(2462..2683)
                     /locus_tag="PLX53_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72152.1"
                     /translation="MQCVEDGLAVIASWRRACRSQPCGNESREGATPSLDLAGIKNLL
                     GCYLYGWLPAYGVMAGRHTELRAGVCFGF"
     gene            complement(2983..>3317)
                     /locus_tag="PLX53_03385"
     CDS             complement(2983..>3317)
                     /locus_tag="PLX53_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72153.1"
                     /translation="EYVVNSKVDVTLKNGLSKQASHFKCDNVDICPSIILKKEDDKWI
                     ENEYAVLCTQMGPMGYFGILNIAETKHDTISLFSEIGRFKLRYRFVIDNDTLDFDSNE
                     FLLYGLEL"
BASE COUNT          960 a          806 c          654 g          897 t
ORIGIN      
        1 cctggttgcg tggccgggtt tcgtcaaccc aaacatgcag ccttacaccc cttcgccatg
       61 cctcgtaaac gggagccagg gctgatccat aatcggtaaa agccagccat cctgcattgc
      121 aatgggttag aatattgacc actccgccat tcttgttgcg ggcaatgtcc tcaattaaac
      181 ctactccctc cagcccaatt ttccgacagc aaccagcatc gtcttcggca atatctatag
      241 ccgtttgacg tgcaatggcc accctatcgt cgggattctc aacccctgca agaacttcca
      301 gctgcctttc aaccgcccat ctcaggttta cggcagtagg acgtgtttta atcagcttta
      361 aggcacactt ctccaacgcc tcctgaaaat tgacaaggtt tctttgctcc cacactgcaa
      421 ggtagatccc aaaggctgcc gctacgccaa ttaagccggc tcccctaagg tgcatttcgg
      481 aaattgcccg tagtaaccca tcaaagcttt caataggctc aactacaaac cgatggggca
      541 ggtatcgctg gtcaattatt ttaacgcatg ggctgctgcc atcctccagc catatcgtcc
      601 gatagttaac tccgtttacc ttcaactctt tctttcgcta atgggttaag gagggcttat
      661 ccactctcat aagcaccacc aaatttaaac taatggcaaa tttacccaaa aacgcgttaa
      721 taaattggaa tgaaattgct tatcagaaaa tcgtggggtt ccttcatggc gttggtataa
      781 tgtatcattg ccttaacatt caaaatagca tgattcataa aaaattgaac ctcttttagg
      841 tcaaaaggtt atattttaaa ggtaatttta cattttttaa aaatctgctg gccatttgca
      901 gcaggtaaat tggatttaaa aaggtacaaa ggctaagcca acatgaacaa gcgtattttc
      961 atactgataa ttatagacct gatgttgata ctggtagctt tctacctatc ggtactgctg
     1021 aaaggggtaa gttttgccag ctacctggag agctaccaga atgcacttct gctctttgcc
     1081 ctgatatgga ttgcaacgtc aatagttttc gagaagtatg tgtttacaac ccattcggcc
     1141 cgaaaaatat cagcaaatat tattaaatca aacctcctgg catttgccgt tgtaactttt
     1201 ctcatatact tcacccggtc cgacaggtac tcccggttta tcgtgttcaa caccattctg
     1261 atggtaaccc tgctggagct attcatatac aacctttggg ccggattaaa gcgaatcagg
     1321 gttatccccg ataatttgct ggggatgatt gagcggagcg taaagcgtcg taccccgccc
     1381 atccaaacaa ccagggcgcc caaccccatg agggttgagc tgattaagcg aacgttacaa
     1441 gcagaatttg ggaacgaggt ttacgagtac ctaaatacca acacccgatt atttgatgat
     1501 aaaacgctga ttgtttccac cactacctca ttcaacataa caaaccagcc cgatggctat
     1561 ttcgacaccc taatcaacct gaaacgaaca aacgacattc gcaggattaa caagtttttt
     1621 gaggcagtta accagaagct gccacggggt ggaatattcg tttgcatggc cgaaacgcag
     1681 gagcagcgca aaaagcgaat actttcaaag tttcccccgg tgataaattg gatatactac
     1741 ttcttcgact acgtagttaa aagggttttc ccgaagttct cgctaaccaa gggtatctac
     1801 ttttttctga ctcgaggtga aaaccgcgtg attagcaggg cagaactgct ggggaggcta
     1861 tactcctgcg gcttttacgt aaataacgag accgaaatag aaaagctaca atacgtggtg
     1921 gccatcaaga accgaaagcc ctttttcgac gaggaaccaa cctacggtcc cattatcaag
     1981 ctacgcaggg taggaaagaa cgggaaaatt atcagggtgt ataaattccg gactatgcac
     2041 ccctactccg aatacctgca ggagtatatt ttcaacctgc ataacctgga ggagggcggc
     2101 aagtttcgaa acgactttag ggtatcaacc cttggtgcag taatgagaag attatggata
     2161 gatgagctgc ccatgattac aaaccttttt cgtggtgagc ttaagatagt tggcgttcga
     2221 ccccttagcg agcaatactt caacctctac acacccgagc tgcaggaacg ccggataaag
     2281 tataaaccgg gtttggttcc accgttctac atggacaatc ccaaaacgct ggaggagata
     2341 atggcctcgg aggtaaagta ccttgatgcc tacgataaac atcctttgtt taccgacata
     2401 cgctacctat tcggggctgc ctacaacata attttcaaaa agtaccgcag cagctaggtg
     2461 cttaaaaccc aaagcaaaca cctgccctaa gctctgtatg cctgcccgcc atgacgccgt
     2521 atgctggcag ccagccatat aagtagcacc ccaacaggtt tttaataccg gctaggtcta
     2581 acgagggggt tgccccttcc ctgctctcgt tgccgcacgg ttggcttcgg caagctctcc
     2641 gccagctggc gattaccgca aggccatctt ctacacactg cataatcctt tttttcgttt
     2701 ttaagcaaat gggagggggt tatgctcata aatcagaaaa atcgcataaa ataagcttcg
     2761 gaatccataa agcagacctt catcaagcta aaaacataca aaaactacct gcaataaatc
     2821 aaagaacgat ttttataatt ccttttttct tcttcaaaat cataaaaaac cggcagaaat
     2881 tgcatagctc tgaatccgtg gggtgtagtt caacatggac tcctcgctgg gtctttcaga
     2941 ccgctcagag tcctatttat tagcgggcgt aatttattac atttagagtt ctaatccata
     3001 caataaaaat tcattagagt caaaatctaa tgtatcatta tcaatcacaa atctatatct
     3061 caatttaaat ctgccaatct cgctaaataa tgatatagta tcgtgtttcg tctcggctat
     3121 attcaaaatt ccaaaataac ccataggccc catttgcgtg caaagaactg catattcatt
     3181 ctcaatccac ttatcatctt cttttttcaa tattatggat gggcatatat caacgttatc
     3241 acatttaaaa tgagaggctt gcttacttag cccattttta agggtcacat caaccttgct
     3301 atttacaaca tattcat
//
LOCUS       DAOOQX010000151         3314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_34464_length_3314_cov_3.205278, whole genome shotgun sequence.
ACCESSION   DAOOQX010000151 DAOOQX010000000
VERSION     DAOOQX010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34464_length_3314_cov_3.205278"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLX53_03390"
     CDS             <1..255
                     /locus_tag="PLX53_03390"
                     /inference="COORDINATES: protein motif:HMM:NF027444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HOY72154.1"
                     /translation="KITKGNIMFAFLTFLFYFFLTLMIIGFLGRLIFRYWMKRVYRKM
                     NGNANETKKRKTTTRQTREKPSKKIINKDQGEYVDFEEID"
     gene            431..1384
                     /locus_tag="PLX53_03395"
     CDS             431..1384
                     /locus_tag="PLX53_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOY72155.1"
                     /translation="MENVVAGIDIGGTNTVVGLVTKSGEILKETTLSTRLETTFDGFI
                     KILVSEIEKLLMTFEHDYKLVGIGIGAPNSSYDLGAIVDAPNLQWKGILPVCKEITKH
                     TGVNSVVTNDANAAAVGEMLFGDAQGMKNFVILTLGTGLGSGIVVDGRLVLGSEGFAG
                     EFGHIVAKPNGRQCGCGKRGCLETYASATGIRRTAFKMLADSNHPSLLRNITYDKLTS
                     KFITEAAKTGDPLARSAFEYTGLILGTRLADLVAILNPEAIFFFGGLANAGELLLDPT
                     RRYMEEYLFPVFRGKVKLLISGLQNKNAAVLGAAALMWKEL"
     gene            complement(1697..>3314)
                     /gene="scpA"
                     /locus_tag="PLX53_03400"
     CDS             complement(1697..>3314)
                     /gene="scpA"
                     /locus_tag="PLX53_03400"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOY72156.1"
                     /translation="GATLEQLSGTIQNDILKEFMVRNTYIYPPEFSMRIIADIFAYTS
                     KLMPKFNSISISGYHIQEAGATADIELAYTLADGLEYIRTGLRAGIDIDAFAPRLSFF
                     WGVGMNHFMEIAKMRAARLLWAKIVKQFNPKNPKSMALRTHSQTSGWSLTEQDPYNNV
                     ARTCVEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYLQEETNICKVVDPW
                     AGSYYVEYLTNELAHRAWALIQEVEELGGMSKAIETGIPKMKIEEAAARRQARIDSGK
                     EIIVGLNKYRLDKEDPIEILNVDNTAVREAQIRRLNKLKAERNNEEVRLALEAITRCV
                     ETGKGNLLELSVEAAKRRATLGEISMACEKVVGRYKAVIRSISGVYSAESMEDQNYAK
                     ARELCEKFAKLEGRQPRIMIAKMGQDGHDRGAKVVATGYADIGFDVDIGPLFQTPAEA
                     AKQAVENDVHVLGVSSLAAGHKTLVPQVIEELKKLGRDDIMVIVGGVIPPQDYQFLYD
                     HGVVGIFGPGTPVSVAAVKILEILLERVTD"
BASE COUNT          892 a          748 c          749 g          925 t
ORIGIN      
        1 aaaattacaa aaggtaatat catgtttgca tttttaacct tcctgttcta cttttttctc
       61 accctgatga tcatcggatt tttgggccga ttaatttttc ggtactggat gaaaagagta
      121 tatcgtaaga tgaatggcaa tgcgaacgaa acaaaaaagc gtaaaacaac caccaggcaa
      181 acccgggaaa aaccctcaaa aaaaataatt aataaggacc agggggagta tgtcgatttt
      241 gaagaaatag actaatttca cccgtttaat ttaatcgtaa ttcttaagtt gtattaataa
      301 acgcttactg cttattagta attcgacgtt atgagcgtat tgcgtgaaat tggtgaaact
      361 aaaggaatgg tttatgcggg ttggcgggta agtctttcta catttaaaaa aattgatttt
      421 attagtgatt atggaaaatg tagtagcagg aattgatatt ggcggtacaa ataccgtggt
      481 aggactggtt accaagtctg gtgaaatact caaggaaaca accctttcta cgcggctaga
      541 aacaactttt gatggtttca tcaaaattct ggtttcggaa atcgaaaagc tgctcatgac
      601 gttcgagcac gactacaagt tggtaggtat aggaattgga gcacctaaca gctcatacga
      661 tctgggtgct attgttgacg caccaaacct tcagtggaaa gggattctcc ctgtttgtaa
      721 ggagataaca aagcatactg gcgttaacag cgttgtaaca aacgatgcaa atgcagcagc
      781 agttggagaa atgctttttg gcgatgcaca gggtatgaaa aattttgtaa ttcttaccct
      841 tggtacgggt ttgggaagcg gcattgtagt tgatggcaga ctggttttag gttctgaagg
      901 ttttgcagga gagtttgggc atattgtagc taaacccaac ggaaggcagt gcggttgcgg
      961 aaaacgtggc tgcctcgaaa cctacgcttc tgcaaccgga attcgccgaa ccgcatttaa
     1021 aatgctggcc gacagcaacc atccaagcct cctgagaaac attacctacg acaagctgac
     1081 ctcaaaattt atcaccgaag ctgcaaaaac cggtgatccg ttggctcgct cggcatttga
     1141 atacaccggg cttattcttg gaacccgctt ggccgacctg gtggccattc ttaaccctga
     1201 ggctatcttt ttctttggag gtcttgccaa tgcaggggag ctgctactcg acccaacacg
     1261 tcgctacatg gaagagtact tgtttccggt atttagggga aaggtaaaac ttctgatttc
     1321 agggctacag aataaaaatg ccgctgtcct gggagccgcc gcattgatgt ggaaggagct
     1381 ataaataacg cttaaatttt tgattttttt cagccgcttc ttttcgacac ctgcgggagc
     1441 ataatttttg caattgaact ggtaaaaaag aggttgttca agagaatcta tgattcccct
     1501 tcattaaagg caaattcaaa catcctgaaa accacaaaat agtggcatgc ctataaagcc
     1561 ccattcggga tgcttcaaat tcacataatt ttctaatgat gaaaggacat gttcatctta
     1621 tgcactttac cctcacacaa aaagaaccaa cagccctagg ggttgttggt tcctacatta
     1681 aaaattgttc caactgttag tcggtaaccc tctcgagtaa aatttcaagt attttcaccg
     1741 cagcaacaga aacaggtgtg cctgggccga atataccaac gactccatgg tcgtaaagga
     1801 attggtagtc ctgtggtgga ataactcctc caacaataac catgatgtca tcacgtccta
     1861 atttttttaa ctcctcaatt acctgcggaa caagcgtttt gtggcctgcg gccaggctgg
     1921 acactccgag cacgtgcaca tcgttttcaa ctgcttgctt ggccgcttcg gctggtgttt
     1981 ggaacagggg gccaatatcc acatcaaatc caatatcggc atagcccgta gcaaccacct
     2041 tagcaccacg gtcgtgccca tcttgcccca ttttggcaat catgattcgc ggctgacgac
     2101 cctcgagttt ggcaaacttt tcgcaaagtt cacgagcttt agcgtagttt tgatcttcca
     2161 tagattcagc agagtaaact cctgatattg aacgaataac agccttgtag cgtcctacca
     2221 ctttttcgca agccatggag atttcaccaa gtgttgcgcg tctttttgcc gcctcaaccg
     2281 atagctcaag taggtttccc tttccggttt caacacaacg ggtaatagct tcaagagcaa
     2341 gcctaacctc ctcgttgtta cgttcggctt ttagcttgtt taaccggcgt atttgcgcct
     2401 ccctaactgc agtattatca acgttaagaa tttcaatggg gtcctccttg tcgaggcggt
     2461 acttgtttaa gccaacaata atctctttcc ccgaatcaat acgagcctgg cgacgggcag
     2521 cagcctcttc aattttcatt tttggaatgc cggtttcaat ggccttcgac ataccaccca
     2581 actcttctac ctcttgtatt agagcccaag ccctgtgggc tagttcattt gtaagatact
     2641 ccacatagta ggaaccggcc caagggtcaa ctactttgca aatgttggtt tcctcctgaa
     2701 ggtaaatctg ggtgttgcga gcaatgcgag cagaaaaatc ggttggaagg gctattgcct
     2761 catccagggc gttggtgtgt aaactttggg tatgacctag cgcagcagcc attgcttcca
     2821 cgcacgttct ggccacgttg ttgtaagggt cctgctcggt taggctccat cccgaggttt
     2881 ggctatgggt tcgtaacgcc atagactttg gatttttcgg gttaaattgc ttaacaattt
     2941 ttgcccaaag caaacgtgct gccctcatct tggcaatttc catgaaatgg ttcatgccaa
     3001 cgccccagaa aaatgacagc ctgggtgcaa aagcatcaat atcgatacct gcccgcagcc
     3061 ctgttctgat gtactccagc ccatcggcaa gggtgtaggc cagctctatg tcggcggtag
     3121 ctcctgcctc ctgaatgtgg tacccggaaa tggaaatgct gttgaacttg ggcataagct
     3181 tcgaggtata ggcaaaaata tcggctataa tcctcatgga gaactccggc ggatagatgt
     3241 aggtgttgcg caccatgaac tctttaagta tatcattctg tattgttccg ctcagctgct
     3301 caagagttgc tccc
//
LOCUS       DAOOQX010000152         3303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_34622_length_3303_cov_3.354064, whole genome shotgun sequence.
ACCESSION   DAOOQX010000152 DAOOQX010000000
VERSION     DAOOQX010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34622_length_3303_cov_3.354064"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..1253
                     /gene="panB"
                     /locus_tag="PLX53_03405"
     CDS             438..1253
                     /gene="panB"
                     /locus_tag="PLX53_03405"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOY72157.1"
                     /translation="MSTESTAKVVTTHVLNEMKLRGEKIAMLTAYDYSMARILDAAGI
                     DVLLVGDSASNVMAGYQSTLPITLDQMIYHASSVVRGVKRALVVVDLPFGTYQGNSKL
                     ALNSSIRIMKESGADAVKLEGGKEILESVDRILSAGIPVMGHLGLTPQSIHKFGTYVV
                     RAREEEEASKLVDDAHRLQDAGCFSIVLEKIPASLGARVAHELKIPIIGIGAGNGVDG
                     QVLVTHDMLGITQEFSPRFLRRYHNLFAEMLGSFQAYIKDVKSLDFPNEREQY"
     gene            1253..1945
                     /locus_tag="PLX53_03410"
     CDS             1253..1945
                     /locus_tag="PLX53_03410"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOY72158.1"
                     /translation="MDFTPDRILFEDNHYIAVNKLCGDIVQADKTGDLSLLETVKQFI
                     KVRDGKPGNVFLEVTHRIDRPVSGVIVFAKTSKGLSRMNHLFQESQVQKRYWAVIRSK
                     PPKDTDLLEHYLVRDSKSNKSFVYNHEVNGSKVARLRYNLVGKSEKYYFLEIELITGR
                     HHQIRCQLAKIGCPIKGDLKYGFPRSNPDGGIHLHSRLFSFTHPISGEQVVITAPPPK
                     DVLWDEFMLLGI"
     gene            2150..3112
                     /locus_tag="PLX53_03415"
     CDS             2150..3112
                     /locus_tag="PLX53_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOY72159.1"
                     /translation="MKFWLVTVKKIAFFFAFTMLLHASAWAQSSMSGSDYIDRYSGIA
                     VRNMKQYGVPASIILAQGMLESNNGNSTLAREANNHFGIKCHKDWTGPTIFHDDDHKN
                     ECFRKYRTPENSFVDHSLFLRGAARYAFLFELEPTDYRGWAYGLKKAGYATNPSYPEL
                     LIKIIEENKLYLYDKGVEVAVASPAKGRGKSVDVDSYEIDIYGGRTVYTRNRIKYIVV
                     KEGDSFESLTKELNLMPWQLFKYNDLTSDSTLRVGEELYIQPKRRKAERNHSVHTVEK
                     GETIYQISQMYGVKLNVLYRRNNMHPGDSTENGQVVFLRGRKPL"
     gene            complement(3107..>3303)
                     /locus_tag="PLX53_03420"
     CDS             complement(3107..>3303)
                     /locus_tag="PLX53_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72160.1"
                     /translation="AAKSVEPTSNLQPGSIDTSSSRTLRVACSDGFIALKIIQQAGKK
                     AMPVEEFVKGWHNTKFIKML"
BASE COUNT          885 a          679 c          806 g          933 t
ORIGIN      
        1 tccaattatt ttgctcattg ttctaagatt atttatttat tgtttacttc aactaccaat
       61 atgtttagca taacctgttt ctgcaatgtt tatgccatgt atttgtggca atgttttttg
      121 gcaaaattgg catttatcgc cacaaacagt aagctttttt ggcagcccgg ttgtttatag
      181 ctgaattgcc attgaaggga aaagaagagg aaaggatagg tgtaacttat tgtacaatct
      241 tgatgaattg catgagatgt gctcattatc tggtcaaaac aacaacccgg gtttttttta
      301 tgggtatatg agcgactctt gtcaggctgc cgttttgtca gtttggcata gtcctgtagc
      361 tactgcccta gccaaaattt caataagttg gattattcct ttacttttgc ctaatcaaat
      421 aattaataca gctgaatatg tcaacggaaa gtactgccaa agtggttaca acccatgtgc
      481 tgaacgagat gaagctgagg ggagagaaga tagccatgct tacagcgtat gattattcca
      541 tggctcgcat tcttgacgct gccggaatag atgttttgct ggtaggtgat tctgcctcta
      601 atgtgatggc cggctaccag tcaactcttc caattacgct agaccagatg atttaccatg
      661 ccagctcggt ggttcgtggt gttaagcgtg cccttgtggt tgttgatttg ccatttggaa
      721 cctaccaggg gaactcaaag ctggcgctga actcatccat acggattatg aaggaaagtg
      781 gtgccgatgc agttaagctg gagggaggaa aggaaattct tgaatcggtg gatagaattc
      841 tatcagccgg cattcctgta atgggccact tgggcctaac gccacagtcc attcataagt
      901 ttggaaccta tgtggttagg gcgcgcgagg aggaggaggc cagcaagctg gttgacgatg
      961 ctcacaggct gcaggatgca ggctgctttt caattgtatt ggaaaagatt cctgcctcgc
     1021 tgggtgcaag agttgcccac gagttgaaaa tacccattat tggtattggt gccgggaatg
     1081 gtgtggatgg acaggtgctg gtaacacatg atatgctggg cataacccag gagttctcgc
     1141 cacggttcct acgtaggtac cataacctct tcgccgaaat gcttggttca tttcaagcat
     1201 acattaagga cgttaagtcg cttgacttcc ccaacgaacg agagcagtac tgatggattt
     1261 cacacccgat agaattctct ttgaggataa ccactacatt gcggtaaata agctttgcgg
     1321 tgatattgtg caggctgata aaaccggtga tctttcgttg ttggaaacag taaagcagtt
     1381 tattaaggtt agggatggca agccgggaaa cgtctttctt gaggttaccc atcgcattga
     1441 caggcctgtg agcggggtca ttgtttttgc aaaaaccagc aagggtctga gcaggatgaa
     1501 ccacctgttt caggaatcgc aggtgcaaaa acgctactgg gcggttatac ggagtaaacc
     1561 tcctaaggat accgatttac tggaacacta cctggtaagg gattccaagt caaataaatc
     1621 atttgtatac aaccacgagg tgaatggctc aaaggtggca cggttgcgct acaacctggt
     1681 tggtaagtct gaaaagtact actttctgga aattgaactt attactggca ggcaccatca
     1741 aatacgatgt cagcttgcca aaattggttg tcccattaag ggcgacttga agtatggttt
     1801 tccccgctcc aacccggatg gtggaattca tctacactcc aggcttttct cctttaccca
     1861 tcccatatcg ggagagcagg ttgttattac agctccacct ccaaaggatg tgctatggga
     1921 cgaattcatg ttgctgggta tatagctgaa tgttgatttc ctgcaatggc ttatattcgt
     1981 tatctttgct gccacaactt aggaaaattc ttaaacaaca ctagtagccg gatatatatt
     2041 agcatgataa caggtgctgg tagccagctt attggacgaa tttatcatgt ataagatacc
     2101 ttgttaggaa ttttaaatga agcataatgg gattaaattg gttattacct tgaaattctg
     2161 gttggtaacc gtaaaaaaaa ttgcattctt ttttgcattt accatgctgc tccatgctag
     2221 tgcatgggcg cagtcatcaa tgtcaggttc agattatatt gacaggtatt ccggtatagc
     2281 tgtcaggaac atgaagcagt atggcgttcc ggcaagtatt attcttgcgc agggaatgct
     2341 ggagagcaac aatggaaaca gcaccctggc gcgcgaggcc aacaatcact tcgggataaa
     2401 gtgccataag gactggaccg gcccaaccat tttccacgac gatgaccata aaaatgagtg
     2461 cttccgaaag taccgaactc ccgagaactc ttttgttgac catagccttt ttcttcgggg
     2521 agcagctagg tatgcctttc ttttcgagct tgaacctacc gattacaggg gatgggccta
     2581 cggtctcaaa aaggccggat acgcaacaaa tccaagctac ccggagttgc taatcaaaat
     2641 aattgaggag aacaagcttt acctctacga taagggggtt gaggttgccg tagcttcacc
     2701 cgccaaagga agggggaaaa gtgttgatgt tgacagctat gagattgaca tctacggtgg
     2761 acgaacggtt tatacccgca atagaattaa gtacattgtt gttaaggagg gtgattcctt
     2821 tgaatcgcta acgaaggagc ttaacctcat gccctggcag ctttttaagt ataacgacct
     2881 aactagcgat tcgacccttc gggtgggaga ggagctttac attcagccta aacggcgtaa
     2941 agccgagagg aatcattcgg ttcatacggt tgagaaaggg gagaccatct accagatatc
     3001 gcagatgtat ggtgttaagc ttaatgttct ctaccgacgc aacaacatgc atccgggtga
     3061 cagcaccgaa aacgggcagg tggtattcct tcgtggtcga aaacctttat agcattttta
     3121 taaactttgt gttgtgccat cctttcacaa actcctcaac tggcatagct tttttccctg
     3181 cttgctggat gatttttagc gctatgaaac cgtctgaaca ggcaactctt aaggtacgtg
     3241 agctacttgt atcaatagat ccgggctgca ggttacttgt tggctcgacg cttttagcag
     3301 cgc
//
LOCUS       DAOOQX010000153         3302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_34638_length_3302_cov_2.771481, whole genome shotgun sequence.
ACCESSION   DAOOQX010000153 DAOOQX010000000
VERSION     DAOOQX010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34638_length_3302_cov_2.771481"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..950)
                     /gene="nth"
                     /locus_tag="PLX53_03425"
     CDS             complement(297..950)
                     /gene="nth"
                     /locus_tag="PLX53_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOY72161.1"
                     /translation="MSTNERYNRFIEYFNRTMPSATTELSYSNPYELLVAVILSAQCT
                     DKRVNMVTPDFIKRFPNPETLAASSTEEVYELVKSCTYPNSKAKHLVEMAQTLLSKFD
                     GKVLEDVQLLQQLPGVGRKTANVVASVAFNIPAMPVDTHVFRVARRLGLVTNAKTPLA
                     VERQLTEKIPAQLLATAHHWLILHGRYVCTARNPKCSECGLADICKEFRSKAKSSEK"
     gene            complement(1191..1409)
                     /locus_tag="PLX53_03430"
     CDS             complement(1191..1409)
                     /locus_tag="PLX53_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72162.1"
                     /translation="MESHHHTNNNTKTPIFGKTQCIVDRIQSLDQKWYVEFWLIRYYF
                     MGIEVYRYKKEKVKLNGKNNELAKNGSE"
     gene            complement(1534..2373)
                     /locus_tag="PLX53_03435"
     CDS             complement(1534..2373)
                     /locus_tag="PLX53_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOY72163.1"
                     /translation="MQSKKPLKLNFSTCPNDTFMFCAMVNRLVETKGYQFEPYLADIE
                     ELNRLAAQQLPDITKLSYGAFPELSASYQLLDSGSALGSGVGPLVVSRRKIYPDEVAY
                     TRAALPGEHTTAHLLFNLAYPNAMNKKFYLFSEIEQAVLDNEADVGVLIHESRFTYMT
                     KGLTKVADLGEFWEKRTNLPVPLGGIAIRKDLPENLKTDIAIILQSSIKFAFANRSAT
                     MPYVRRFAQEMDEEVMEKHINLYVNNFSLSLGNSGRTAVLELLKTSGKLPDNCETKSL
                     FIY"
     gene            complement(2376..3038)
                     /gene="mqnB"
                     /locus_tag="PLX53_03440"
     CDS             complement(2376..3038)
                     /gene="mqnB"
                     /locus_tag="PLX53_03440"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HOY72164.1"
                     /translation="MNRILLVLATQVEAMPIATKKLPIGFPTAMSLTERHHVDILVTG
                     AGAVPTAYHLARNLSTYNLIINLGIAGSYRAEIMPGSLVTLGSDAFADYGIDNNGHFE
                     PINSFDFNNDTPYRLDRLESPWLNQFNLALPVVKGITVGTTSGSTERIARLREQWNPD
                     IETMESAAVFFACLAAGKPFICLRAISNAVEPRNPKNWRIKLAVDRLADCTLDLLENK
                     IA"
BASE COUNT          822 a          679 c          775 g         1026 t
ORIGIN      
        1 atttgtacgt tacttttagg tttatgattt tcataccctt taggcatggg ggttaagcgg
       61 aaaggatacc catgtttagg tttcgccgaa ctcgtaggta acgcacggtt attcatgctt
      121 gtttatgcaa gttaccctac ctggttgttt aataaagaat agctgcaaga aattacctgt
      181 agttcagggt tccatttgaa gccacttctt tgcatgggta catccccggt atttgggcat
      241 aatatttttt tgatgttctc cgaaccttag atggttgact actgtatagc tggcttttat
      301 ttttctgaag attttgcctt gctgcgaaac tctttgcaaa tgtcagctaa gccacattcg
      361 ctacattttg gatttcgggc tgtacaaaca tagcgtccgt gtagtataag ccaatggtgc
      421 gctgtagcaa gcagttgggc cgggattttt tcagtaagct gccgttcgac tgccagcggt
      481 gttttggcat tagtaaccaa acctaaacgt ctggcaaccc tgaaaacatg cgtatcaaca
      541 ggcatggcag gtatgttaaa agctaccgag gccactacgt ttgctgtttt acgcccaact
      601 cccggcagct gctgtagcag ttgaacatcc tcgagcacct tgccgtcaaa cttggagagc
      661 agggtttgag ccatttccac cagatgcttg gccttgctat tggggtaggt gcaacttttc
      721 accagctcgt acacctcctc ggtgcttgat gctgccagcg tttcagggtt ggggaagcgt
      781 ttaataaaat caggagttac catgttcaca cgcttgtcgg tgcactgggc gcttaggatt
      841 actgctacca gtaactcgta ggggttgctg tagctcagct ccgttgtggc cgacggcatg
      901 gttcggttaa agtactctat aaatcggttg tagcgttcgt ttgtagaaat catcaggtat
      961 atggcaatat ttttttcaaa tatactgaag gcagggcaac aaaatcttgt tatttatctc
     1021 agaaatatcc aaaatttact atcccgttaa cgttggttgt attgagatgg tcgcactatg
     1081 gctttttata aataatcttt gagttgatca tccatctatt gcttccggaa aagaaaaaat
     1141 gggatatgga tggctattta agaggtaagt attagatttt agcgtggatt ctactcgcta
     1201 ccattcttgg caagctcgtt gtttttccca ttcagcttaa ccttctcctt tttgtacctg
     1261 taaacctcaa ttcccatgaa gtagtaacga atcagccaga attctacata ccatttttgg
     1321 tcgagcgatt gtatcctgtc aacaatgcat tgagtttttc caaaaatggg tgttttggtg
     1381 ttattgttgg tatgatggtg actttccatt gagcatttaa aaagttattg taatcataaa
     1441 aatatcgttt tcaaatcaaa gtttaaaggg tttgagctgt aaaaattcaa attatttggc
     1501 gaaaagcagc ttgcgcaatg ccaaggctat cattcagtat atgaaaagag atttggtttc
     1561 acagttgtcc ggcagttttc cggatgtttt aaggagctcc agcacggcag ttctgcccga
     1621 gttacccagt gatagagaga agttgttgac gtagaggtta atatgttttt ccataacttc
     1681 ttcgtccatt tcctgggcaa agcgtctgac atagggcatg gtggcagagc gattggcaaa
     1741 tgcaaacttt atactgcttt gcaatattat ggcaatgtcg gttttcaggt tttcaggtaa
     1801 gtcttttcta atggcaattc ccccgagggg tacgggtagg ttggttcttt tttcccagaa
     1861 ttcacctaaa tcggctacct tagttagccc cttggtcatg taagtaaatc gtgactcatg
     1921 gatgagcaca cctacatctg cctcgttgtc cagcacagcc tgctcgattt cggaaaaaag
     1981 gtagaatttt ttattcattg cattggggta ggcaaggttg aatagcaggt gggctgtagt
     2041 atgttcgccg gggagagcag ctctggtgta ggccacctca tcggggtaaa ttttacgtct
     2101 gctaactacc agagggccaa cgccgcttcc caaggcgcta ccggagtcca gcagctggta
     2161 gctggccgaa agctccggga atgccccgta gcttagcttg gtgatatccg gtagctgttg
     2221 tgctgctagc cggttcaact cctctatgtc ggccaggtag ggttcaaact gatacccttt
     2281 ggtttctacc aagcggttaa ccatagcgca gaacataaag gtatcgttgg ggcaggtaga
     2341 aaagttaagt ttaagaggtt ttttgctttg catgattatg ctattttatt ttccagcagg
     2401 tcgagggtgc aatcggctag cctatcaacg gccagtttaa ttctccagtt ttttgggttt
     2461 cggggctcaa cggcgttgga aatggcgcgc aggcaaatga aaggcttgcc ggcagcgagg
     2521 caagcaaaaa agacagctgc cgactccatg gtttcaatat cgggattcca ttgttctcgc
     2581 aacctggcaa tcctttctgt tgaaccgcta gtagttccaa cagtaatgcc ctttacaaca
     2641 ggaagcgcta ggttaaattg gtttaaccag ggactttcta gcctatcgag ccggtaagga
     2701 gtgtcattgt taaaatcaaa actattgatt ggctcgaaat gcccgttgtt atctatccca
     2761 tagtcggcaa atgcgtcgct acctaaagta accaagctgc ccggcataat ttctgcccgg
     2821 tagctaccag ctatgcctaa gtttataatg aggttgtagg ttgataaatt cctggccaga
     2881 tgatatgctg tgggcactgc accagctccg gtaaccagaa tatctacgtg gtgtctttcc
     2941 gtaaggctca tagccgttgg aaagcctatg ggtagcttct ttgtggctat tggcatggcc
     3001 tcaacctggg ttgccagaac tagaagaatt cggttcatgt tcttaaattc gcctgattac
     3061 cgttttgaac aataaaattc ctaaaatgtt aaactgccta catgagtcgt atagtttcat
     3121 tttagctcat caaaattaca aaaagcgtat aactttaaat ttaagagctt agttttgtag
     3181 tttgttttcg ctaaacagtg gtttatatgc ctgttgttta cctaacacga agggagcgtt
     3241 ttagctcggc ccaccgtctt tttaatccgg atttgagcga tgagcaaaat cttcaaattt
     3301 tt
//
LOCUS       DAOOQX010000154         3298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_34704_length_3298_cov_3.406722, whole genome shotgun sequence.
ACCESSION   DAOOQX010000154 DAOOQX010000000
VERSION     DAOOQX010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34704_length_3298_cov_3.406722"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLX53_03445"
     CDS             <1..416
                     /locus_tag="PLX53_03445"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HOY72165.1"
                     /translation="AFLPLQKRLKQWSDRKKLVEEPLFRSYIFVRIDAREYYEVLNTP
                     GIVRYITFEGKAVPIPDRQIEQVKQLLVQDLEIEPVEEQIPPGALVEVRFGNLMGITG
                     ELVEHAGKKKVIIRIDHVSHSLLVTLPKDYVARAV"
     gene            complement(461..1189)
                     /gene="pepE"
                     /locus_tag="PLX53_03450"
     CDS             complement(461..1189)
                     /gene="pepE"
                     /locus_tag="PLX53_03450"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HOY72166.1"
                     /translation="MDSQSKKTVKMLLISNSTNAGEAFLEHPKEELKRFLNGTKRVLF
                     IPWAAVTFSYDEFERKVQERFSEFGVEVDSIHHYRNVKTAVQEAEAIVVGGGNTFKLL
                     KTIQQHDIVETVRNKVLDGTPYIGWSAGANVACPTICTTNDMPITEPDNFGAFSLIRF
                     QINPHYLDANPAGHAGETREQRIMEFLEMDPYVYVVGLREGSMILLENVALTLKGPKS
                     ARIFKKGIPPFEVNPGDDMDFLFE"
     gene            complement(1226..3175)
                     /locus_tag="PLX53_03455"
     CDS             complement(1226..3175)
                     /locus_tag="PLX53_03455"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY72167.1"
                     /translation="MKGSLAAILLAGSLSLVTPRLWGQPLSLDSLLHELSTVKSSEGK
                     YKILSSIAISYSDSSYSKSLEYWLEALEVAKKLESSKYTADILHQIGFTYLKMGEPKS
                     ALANFQNAAELYVSLNDNADLAGVYNDMGLIYRSWGRYDSALEFYLKSYKLYERLANA
                     EGMAMASNSIGQIYYYRENYTKAVDYFSSYLEINQGLGNYRAVAGACNNIASAYMELN
                     HLDRALDYYMKAFKIYDSLGVKIGVGIIQDNIGSLLYSKGDYRNALLYHFNALQIFNE
                     LKSDTRKCLTLKNIGSVYLKQNRPDVAVAYLNQSLDMAVGTGQKDVARDVHQTLANAY
                     EVIGSFPKAYYHLKNYQVLKDSMLNAETIEKIETLQAQLEMENQAAQVSLVQHKLKLQ
                     RQVAWTVSGLLAMMGVLLVLLYRENKKKNAALAEEQKFKESTVKRLDAFLQNTLKVAD
                     DGTVGGFFSDSWKLQPANSTQGTCKLLSFKVNNSTLAFMQVSDSQRVSPNLITLCLCC
                     WVKSNAHRLMISWVNFMKEMRENFAADPLTSDLDPSQYCILPFTINGDFVLNLTEEGF
                     AVWHSNKLIFPKAFQCPELVKGDTLYLYFTTIGLPLPGREQREMEKIIGSVASMEFSL
                     QPQAMQNLLKTFELENFTYIFALRI"
BASE COUNT          883 a          738 c          720 g          957 t
ORIGIN      
        1 aagcctttct gcccttacag aaaaggctaa agcagtggag cgataggaaa aagttggtgg
       61 aggagccttt gttccggtca tacatttttg ttcggattga tgcccgggag tactacgagg
      121 tgttgaacac tccaggaatt gttaggtaca tcacctttga gggaaaggcc gttcccattc
      181 ccgacaggca gattgagcag gtaaaacagc tgctggttca ggatttggaa attgaaccgg
      241 ttgaagagca aattccacca ggagccttgg ttgaagttag atttggtaac ctgatgggga
      301 ttaccggtga gctggttgaa catgccggaa aaaagaaggt tattatccgt atcgatcatg
      361 taagccactc cctgctggta acccttccta aggattatgt agcacgagcc gtgtaggaac
      421 tatttccccc gtattgcttt tctcaacagg gctggtagtg ctattcgaat agaaaatcca
      481 tgtcatcacc cgggttaacc tcaaatgggg gaataccttt tttgaatatt ctggcagatt
      541 ttggtccttt tagggtcaga gcaacattct ccagcagaat catcgaaccc tcccgcaaac
      601 caaccacgta aacgtatgga tccatttcga ggaactccat aatgcgctgc tcccgggttt
      661 cgccagcatg tcctgcgggg tttgcatcca ggtagtgggg gttaatctga aagcgaatca
      721 ggctaaaggc accaaagtta tcgggttcgg tgatgggcat atcgttggtt gtgcaaatgg
      781 tggggcaggc cacgttggct ccagcactcc agcctatgta gggggtgcca tccaaaacct
      841 tattccgaac agtttcaact atatcatgct gctgtatagt cttaagcagc ttaaaggtgt
      901 taccaccacc aacaacaatg gcttcagcct cctgaaccgc cgttttaaca tttcgatagt
      961 ggtgaatgga gtcaacctca acgccaaact cgctgaagcg ttcttgtacc ttacgttcaa
     1021 actcatcgta gctaaaagtt acggctgccc acggaatgaa aagcaccctt ttagtgccat
     1081 tcagaaacct tttaagctct tcctttggat gttccagaaa cgcttcccct gcatttgttg
     1141 agttgcttat tagcagcatt ttaacggtct tctttgactg gctatccata gtaattagtt
     1201 gttataagaa ttaattctca aatttttaaa ttctaagggc aaatatatag gtaaaatttt
     1261 ccagctcaaa cgtttttagc aagttttgca ttgcctgggg ttgcagggaa aattccatag
     1321 acgcaacact gcctattatt ttttccattt ctcgctgctc tctacctggc aagggtaaac
     1381 ctattgtagt aaagtaaagg taaagagtat cacccttcac aagttctgga cattggaacg
     1441 ctttggggaa tatcagctta ttgctatgcc atacggcaaa accctcctct gtaaggttca
     1501 aaacaaagtc tccattaata gtaaaaggca aaatacagta ttgtgaaggg tcaagatcac
     1561 tagtaagcgg gtcagctgca aaattctccc tcatctcttt cataaaattt acccacgata
     1621 tcatgagccg atgggcattg cttttaaccc agcagcacag gcatagagta atgaggttgg
     1681 ggctaactct ttggctatca cttacttgca tgaaagcaag ggtagaatta tttaccttaa
     1741 agctgagcaa cttgcaggta ccctgggtgc tgttagctgg ttgcaatttc cagctatcgg
     1801 aaaaaaatcc tcccactgtg ccatcatccg ccacctttaa agtgttttga agaaatgcat
     1861 ctagccgttt tactgtgctt tctttaaatt tttgttcctc ggcaagagct gcgtttttct
     1921 tcttgttttc cctgtagagc aaaacaagca aaacccccat catggctaac aagccactca
     1981 ccgtccaggc aacctgccgc tgtagcttta acttgtgctg aaccagagaa acctgcgctg
     2041 cctgattttc catttcaagt tgtgcctgca gggtttcgat tttctcaatg gtttcagcat
     2101 ttagcatgga atcctttaaa acctgatagt tttttaagtg gtaataggct ttaggaaagc
     2161 ttcctattac ctcatatgcg tttgctagcg tttggtgcac atctcgtgca acatcttttt
     2221 gacctgttcc aacagccata tccagcgact ggttaaggta ggctaccgca acatcggggc
     2281 ggttttgctt caggtaaaca cttccgatgt ttttaagggt aaggcattta cgcgtatcgc
     2341 ttttcagctc attaaagatt tgaagagcgt taaaatggta aagaagggcg ttacggtaat
     2401 ctcccttact atacaagagc gatccgatat tatcctgtat gataccaaca cctattttta
     2461 cccccaacga atcatagatt ttgaatgctt tcatgtagta atcaagcgct ctatcgaggt
     2521 ggttgagctc catgtaagcg ctggcaatgt tgttgcatgc ccccgcaacg gctctatagt
     2581 ttcctaaacc ttggttaatc tccaggtagc tggaaaagta atcaactgcc ttagtgtagt
     2641 tttcgcggta gtagtatatt tggccaatgc tgttggaagc catagccatc ccttcggcgt
     2701 tggctaacct ttcgtaaagc ttataggatt tcaaatagaa ctccagcgcc gaatcgtacc
     2761 ttccccagct gcggtaaatc aagcccatat cgttgtaaac tcctgccaga tcggcattgt
     2821 catttagaga gacgtacagc tcagcggcat tttgaaagtt ggcaagcgca cttttgggct
     2881 cgcccatttt taggtaggta aatcctattt gatgtaggat gtcggcagta tatttactgc
     2941 tttcaagttt ttttgccacc tccagcgctt ctaaccagta ctccaagctc ttgctgtagc
     3001 tgctatcgga ataggaaatt gcaatgctgc taaggatttt atactttccc tcgctggatt
     3061 ttacggttga aagttcatgg agaaggctat ctaaagacaa tggctgtccc catagccttg
     3121 gtgtaaccaa ggagagcgat ccggcaagca atatggctgc taaactccct ttcaaggcat
     3181 cccttcacaa gataaagaca ggttaactat ttaataagcg gcttggcttg aaagttacta
     3241 gtttcaaagg aaaaaacaaa tttaaagtat agcgataaac agcaccatcc taaaacaa
//
LOCUS       DAOOQX010000155         3296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_34741_length_3296_cov_3.012650, whole genome shotgun sequence.
ACCESSION   DAOOQX010000155 DAOOQX010000000
VERSION     DAOOQX010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34741_length_3296_cov_3.012650"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(401..3025)
                     /locus_tag="PLX53_03460"
     CDS             complement(401..3025)
                     /locus_tag="PLX53_03460"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOY72168.1"
                     /translation="MELASKYNPAEVEEKWYEYWNRHRLFSSKPDSREPYTVVIPPPN
                     VTGVLHMGHMLNNTIQDILVRRARMEGKNACWVPGTDHASIATEAKVVAKLKADGIEK
                     SQLSREEFLKHAWEWKEKHGGIILEQLKKLGASCDWSRTRFTMDETLSASVIHVFVDL
                     FNKGYIYRGVRMVNWDPQAKTAVSDEEVVYREVKGKLYFVRYRIEGEDSFVTIATTRP
                     ETILGDTALCVHPDDPRYTHLKGKRVIVPLVNRSVPIIYDDYVDREFGTGALKITPAH
                     DVNDYLIGQKHNLDSIDIFNDDGTISSAAKLYVGLDRFEVRRRIVDDLESSGNLERVD
                     DYVNSVGFSERTDAIIEPKLSMQWFLRMKELAEPALKAVVEGDIQLYPPKFVNTYRHW
                     MENVKDWCLSRQLWWGHRIPAWYLPNGGFVVAHNVEEAVELARKAAGNSGLVADDLRQ
                     DEDVLDTWFSSWLWPISVFNGILEPNNPDIRYYYPTNDLVTAPEILFFWVARMIMAGY
                     EYMGDKPFRNVYLTGIVRDHLHRKMSKQLGNSPDPLKLIEKYGADGVRVGMLLCSPAG
                     NDLLFDENLTEQGRNFCNKIWNAFRLVKGWQVDESAVQPQSAIAAIEWFNSLLNKSIE
                     QADDDFAKYRISEALMNLYKLFWDDFASWYLEIIKPAYGKPVDAVTYNATVSIFEKLL
                     IMLHPFMPFITEELWQHIRQRGDGESIMVQQLPHSTGFNQDILIGFDRVKEVVVGVRN
                     IRQENNISPKQPLQLSIRNAAKLDAGLEEIISKLASINSLTYIDQKIEGAATFMVGTT
                     EIYIPLGGLINTEEEREKILSELEYTKGFLEKVMKKLNNERFTSNAPKAVVELEHKKQ
                     ADALAKIEALQERLKSLG"
BASE COUNT          876 a          777 c          713 g          930 t
ORIGIN      
        1 tcttagcctc acaaaagcgg atgatgtcat tcttggtaaa ctcagctgaa ggttttttaa
       61 gaaaccgaac cagctcattg gggttcatca aaatttcaga atcgttcatt ttaaccggtc
      121 ttagattaat attttacaaa attacgattt tacagattac agatttaaag gatttcggat
      181 gtttcataac ataatttcaa atccgcaggc tcaaaatgga aaaaccgtaa ccaaacttta
      241 cattttaaag ggcagataga agtaaattca aaaaaagagg tgagcatttt ggatgaatat
      301 tccaaaatga tattccactc aaagctataa atcattacag cttgttacag gcagagaatg
      361 attgcgctat gaattagtaa tattctaaag ttcgagggca ctaccccaat gacttcaagc
      421 gttcctgtag agcttcaatc tttgccaggg catcggcttg ttttttgtgt tccaactcaa
      481 caacggcttt aggcgcattg cttgtaaaac gttcgttgtt aagctttttc attacttttt
      541 cgagaaatcc tttggtgtac tcgagctcgg aaagtatttt ttcccgttcc tcctcggtgt
      601 taattaaccc tcctagcggg atgtaaatct ccgtagtgcc caccatgaac gtagctgcac
      661 cctcaatttt ctggtcaatg taggtcaacg agtttatcga ggccagctta gaaatgatct
      721 cctcaagacc agcatcgagc ttagcggcat ttcgtatgct cagctgtagg ggttgcttgg
      781 gtgaaatgtt gttttcctgg cgtatattcc taacacccac gacaacctct tttaccctat
      841 cgaaaccgat tagaatatcc tggttaaatc ctgttgaatg gggtaattgt tgaaccataa
      901 tgctttctcc atcccctcgc tggcgtatgt gttgccaaag ctcctcggta ataaatggca
      961 tgaatggatg aagcataatc agcaacttct cgaatatgct tactgttgcg ttataggtaa
     1021 cggcatctac gggtttacca tatgcaggtt taataatttc gaggtaccat gacgcaaaat
     1081 catcccagaa aagcttgtag aggttcatga gtgcttccga aatgcggtac tttgcaaagt
     1141 catcatcagc ctgctcaatt gacttgttca gcagggagtt aaaccattca atggcagcaa
     1201 tagccgactg cggttgaact gcagattcgt caacctgcca gcccttaacc aaccggaagg
     1261 cgttccatat tttgttgcag aagttacgac cctgctcagt caggttttcg tcgaaaagca
     1321 ggtcgtttcc ggcaggtgag cataaaagca ttcccaccct aaccccatcg gccccgtact
     1381 tctcgatcaa ctttagcgga tcgggtgaat ttccgagctg cttgctcatt ttgcgatgca
     1441 ggtgatcacg aacaatcccg gtgaggtaaa cattccggaa aggtttatcg cccatgtact
     1501 cgtaaccggc cataatcatt cgggcaaccc agaagaaaag tatttcgggt gcggtaacca
     1561 ggtcgttggt tgggtagtag tacctgatgt cggggttgtt tggctcaaga ataccattga
     1621 aaaccgagat tggccagagc cacgagctaa accatgtatc cagcacatcc tcgtcctgtc
     1681 taagatcgtc ggccactaga ccggaattac cggctgcttt ccgtgccagc tctaccgctt
     1741 cctcaacatt atgagcaacc acaaagcccc cattgggaag gtaccatgca ggaattctat
     1801 gcccccacca aagctggcgt gaaagacacc aatcctttac attttccatc cagtgacggt
     1861 aggtgttgac aaacttagga ggatagagct gaatatcgcc ctccacaaca gccttgaggg
     1921 ctggttcagc caactccttc atgcgaagga accactgcat cgaaagctta ggctcaatta
     1981 tggcatcggt tcgctcgcta aagccaacgc tgtttacata gtcatctacc cgttcaaggt
     2041 ttcccgagct ttctaaatca tcaacaattc tccttcgcac ctcaaatcta tccaaaccca
     2101 cgtatagttt tgctgcggaa ctaattgtgc cgtcatcgtt gaatatgtca atagaatcca
     2161 ggttatgttt ttgaccaatc aggtagtcgt ttacatcgtg agcgggagta atcttgagag
     2221 caccggtgcc aaactccctg tcaacataat catcgtaaat aattggaacg ctacggttta
     2281 ccagcggaac aatcactctt ttacctttca gatgggtgta tcgggggtca tcggggtgaa
     2341 cacaaagggc tgtatcaccc agaatggttt cgggtcttgt ggttgcaata gtaacaaacg
     2401 aatcttcacc ctcaatcctg tagcgtacga agtaaagctt tcccttcact tcgcggtaaa
     2461 ccacctcctc gtctgaaacc gccgtttttg cctgcgggtc ccagttcacc atgcgaacgc
     2521 cgcggtatat gtatccctta ttgaaaaggt caacaaaaac atggataaca ctagccgaaa
     2581 gggtttcgtc catggtaaag cgggtgcggg accagtcgca tgaggccccc aactttttca
     2641 gctgctcaag aataatcccc ccgtgttttt ccttccactc ccacgcatgt ttaaggaact
     2701 cctcacggct tagctgtgat ttttcaattc catcggcttt cagcttagcc acaacttttg
     2761 cctcggtggc aattgaggca tggtcggtac ccggcaccca gcaggcattc ttgccttcca
     2821 tgcgggcacg gcgcaccaga atatcctgaa tggtattatt aagcatgtgt cccatgtgca
     2881 acactccggt tacgttggga ggaggaatga cgacggtata gggctcacgg ctatcaggtt
     2941 ttgaactgaa taaacgatga cggttccagt attcatacca cttctcctca acctcggcag
     3001 gattgtactt gcttgctaat tccattttat tttatctgtt aattatttat tgcaacttca
     3061 tgcatactat tgttttatag tatagtggtc gcaaaattaa taaaaataag tagaaaaaag
     3121 cagcgggaat gcgtactttt gcttttgctt aacaagaaaa aggatgatac gtaagaaaca
     3181 actgattagg tatatcgttc tgggtattct tttgggcttg gtgctcatgg tccaaagcat
     3241 tcccgcgtgg gaggaagtct acgcccgttc tgtgtatccg gtaataggtg cactat
//
LOCUS       DAOOQX010000156         3288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_34840_length_3288_cov_3.946799, whole genome shotgun sequence.
ACCESSION   DAOOQX010000156 DAOOQX010000000
VERSION     DAOOQX010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34840_length_3288_cov_3.946799"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PLX53_03465"
     CDS             <1..835
                     /locus_tag="PLX53_03465"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOY72169.1"
                     /translation="RGAERSRDPQTIIHEVEELVRNGYREVTLLGQNVNSYSWSESKG
                     MVKHTVNFADLLEMVALVSPFLRVRFSTSHPKDLTNDVLYTMAMYENICNHIHLPVQS
                     GSDRILQLMNRKYSRNSYLNRIQAVREIIPDCAISTDIIAGFCTETELDHDLTLSLMR
                     EVGFDFAYMFKYSQRPNTKAARHLVDDVPDEIKSRRLTEIIELQGKLSEESKKKDVGK
                     RFEVLVEGTSKKSDDFYVGRTTHNKVVVFPKEASSIGDFVLVEVTSCTPATLIGKIVD
                     E"
     gene            complement(890..1660)
                     /locus_tag="PLX53_03470"
     CDS             complement(890..1660)
                     /locus_tag="PLX53_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72170.1"
                     /translation="MSKINLTPGEINDLIDLYQTEIDRTQRRIENLKSILKKIDRAQE
                     EVSEPASRVAAPARRGRKKLVESVPAAGEAVTPEAKVDGRKVRKGRNAKAVAKSAAPK
                     VKAIRKTRKSKANAATSEETKLAATKRGRKANLKSKVAAKKSPRKESVVRKKIKSGAG
                     PDKVKWIDLIQDILRSKQNLMLANSLTVEAMERLGIDESDRDRVRMAVATNLTRLTKN
                     LKLVVKFKPEGSTNYFYGLTEWFDEGGNLKSEFQNKLM"
     gene            3109..>3288
                     /locus_tag="PLX53_03475"
     CDS             3109..>3288
                     /locus_tag="PLX53_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72171.1"
                     /translation="MLLVALGSSLWAQNSSNPLWMRYPAISPDGKHIAFSYMGNLFRV
                     SSDGGMAELLTSNAAY"
BASE COUNT         1028 a          686 c          622 g          952 t
ORIGIN      
        1 ccgaggagca gaacgcagcc gagatcccca aaccattatc catgaggtag aagagctggt
       61 tagaaacggg taccgggagg ttacgctgct tggtcaaaat gtcaactcat attcctggag
      121 cgaatcgaag gggatggtaa agcacaccgt taactttgcc gaccttctgg agatggttgc
      181 cctggtgagt cctttcctaa gagtgcggtt ttccacttcc catccaaaag acctgaccaa
      241 tgatgtgctt tacaccatgg caatgtacga gaatatttgt aaccacatcc atctacccgt
      301 gcagtcaggc tcagaccgca ttctccagct gatgaatcgc aagtactccc gcaacagcta
      361 cctaaacaga atacaggccg tgcgggaaat aattcccgac tgtgctatat caaccgatat
      421 tattgcaggt ttttgcactg aaaccgagct cgatcacgac ctcaccctaa gcctgatgag
      481 ggaggttggg tttgactttg cctacatgtt caagtactcc caacgcccaa atacaaaagc
      541 ggccaggcat ttagttgatg atgtacccga tgaaattaaa tcaaggcgct taaccgaaat
      601 catcgagcta caaggtaagc tgtcggaaga aagtaagaaa aaggatgttg ggaaacgatt
      661 cgaggtgctg gttgaaggca cctcaaaaaa atcggacgat ttctatgtgg gtcgcacaac
      721 acacaataaa gtggtggtgt ttccaaaaga agcaagtagt attggcgatt tcgtgctggt
      781 tgaggttacc agctgcacgc cagctaccct aattgggaag attgttgacg agtagtagta
      841 aaaaaggccg tataaaacgg ccttttttgg ttgaaataat actctaacac tacatcagct
      901 tgttctgaaa ttcgctcttt aaatttcccc cctcatcaaa ccactcggtt agcccgtaaa
      961 agtaatttgt actcccctcg ggtttgaact tcacaaccaa cttaagattt tttgttaagc
     1021 gggtaagatt tgtagccaca gccattctta ctctatccct atccgactcg tcgataccta
     1081 aacgctccat tgcctcaacg gtgagagagt ttgccagcat caagttttgc ttgctacgaa
     1141 gaatatcctg aattagatca atccatttta ccttgtcggg accagcaccc gacttaatct
     1201 tctttcgtac gacactttcc tttcggggcg atttcttagc ggcaactttt gatttcaaat
     1261 ttgcctttct cccccttttt gtagcagcca gcttggtttc ttcggaggta gcagcattag
     1321 ccttactttt ccttgtcttt ctaatggctt ttactttagg ggcagcactc ttagcaacgg
     1381 ctttcgcatt ccggcctttt cgaacttttc taccatcaac cttggcctcg ggtgtaactg
     1441 cttcaccggc tgctgggact gattcaacca attttttcct ccctcttctt gctggagcag
     1501 caacccggga ggcgggttcc gaaacttctt cttgtgcccg atcaatcttt tttagaattg
     1561 attttaaatt ttcaattcta cgttgagttc tgtcaatctc ggtttggtag agatcaatca
     1621 aatcgttaat ctctcctggg gttaaattta ttttactcat cttttagtgc tattaaaaat
     1681 taactgatta gttgaaaatt tcattctaaa actgttacca ttactaaata aaataaaggt
     1741 gaaaacaaaa aaatatctat aaaaagaaaa taataatttt tataatttaa ctttttatca
     1801 acaaatcaac tagttaataa gtttcaaaaa tagataaaaa aatattatat aagtacaact
     1861 tgtgtgattt tttttatttt aattatcaat gtttaattaa atccctaatg ttgatgagct
     1921 ctctcatcgt tttcaaaata accatttatg aaaggcgtat gtagtgaaat tgatttacaa
     1981 atatcaacga aattaatata tttagctgat tattaactca tagcaacatt tttaatacat
     2041 gacttcagag tagcgatttt atttttctac atgcaagcaa ccatccaaag caatcagacg
     2101 gtttaatatt agccaggttt aattgtgaag ttgaacttga agaaaaatca cattaaaatt
     2161 tttcaggata accctctaaa aaagggaagg aaggcaaaag ccactttaca tgaaaattat
     2221 taagtataaa aaacgcttaa actttggtag gcaagatata cctgtggagt aggtgcccgt
     2281 taaaccaaat gcaccaagac ttatcaaaga accgaaataa aacacgagaa ctgaatgtgt
     2341 tattcgagct agcctcacca tgtgtatgca ttttcccctg ggactacaag gcttacctgt
     2401 tcactttaaa tagtacagct aataccgaat taaaatattt caaccgccat gcaaagcgca
     2461 ctccagtttg tcaacataac ataaattttt aaaaaagtgg taaattgtaa accatagtaa
     2521 tagctctatc agcctgaacc aagagcattt gcctaagaaa aagtccgagg aaatgttgaa
     2581 tgaagtaaat ttgggttaca aagaatcttc aagagatggc tcgtttgtga gttttaccag
     2641 caaagccatt attttaaaac gacaaaatgc taatgattgc aacaataagt aaactggtag
     2701 gttggcttta ttttttcgga ctagttctct attcatatta atactggcta ataaatataa
     2761 agatgtaaag aatcatgatt aagcaggttg aaatccaaaa cttcaaacct aggtgtttcc
     2821 cggccaaact tcttatccaa ggcatccagc aagcatggcc tgctaccatg gtaaatcagc
     2881 atatatacca gcgaaataat aacccaataa aatttcaggt ggaacattca tgcagggcag
     2941 cttgcacaaa cgcgccggaa caaccaggcg atactagtaa gttcggcgag gccaaaaaat
     3001 gggaattcca tctgttcaac tccataccta aatggtatgg ggcaaagtac ttgtaatcaa
     3061 aaacctaaaa atttttcacg aatgaaaaga aaatctttca aaacagctat gctcctggtc
     3121 gccctaggaa gtagcttatg ggcacaaaat agttcaaatc ctctatggat gcggtatcca
     3181 gctatttcgc ccgatggcaa acacattgcc ttttcatata tgggcaacct atttagggtt
     3241 tcgagcgacg gcggaatggc agaacttctg acctccaatg cagcgtat
//
LOCUS       DAOOQX010000157         3277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_35001_length_3277_cov_2.862818, whole genome shotgun sequence.
ACCESSION   DAOOQX010000157 DAOOQX010000000
VERSION     DAOOQX010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35001_length_3277_cov_2.862818"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /gene="lptE"
                     /locus_tag="PLX53_03480"
     CDS             <1..322
                     /gene="lptE"
                     /locus_tag="PLX53_03480"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOY72172.1"
                     /translation="NRFITQTSLSMVDDFGDFDFSGQITQYNVSPISIQDNEVAAQNR
                     LTISVRVKFVNAKDPKLSFDKTFSQYENFDSNQNFVQVEAELVRLIVEKLVDDIFNAA
                     AANW"
     gene            327..1097
                     /locus_tag="PLX53_03485"
     CDS             327..1097
                     /locus_tag="PLX53_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72173.1"
                     /translation="MDNETLYGIMEARLKPTPETLNQLRETIDEFPYFQGCRVLILKS
                     LSQLGNDEFVAQLRKNAVLLPSRRYLYFTLYPSKPLEETEKIDGGNAEDKKISKPTPK
                     RGRPRKAPTDERKPSFILLDDEKQPAITIHVEASSNTTDTEILELAEGEGEVDSQANG
                     RGLNDSEDLIAKFINNQPTISRSTTPGNKDTGENEDISISSVEEPEELASESLAQIYL
                     SQGYLEKAIRVFEKLSLKYPEKSSYFADEIRKIKESTK"
     gene            1120..1467
                     /gene="secG"
                     /locus_tag="PLX53_03490"
     CDS             1120..1467
                     /gene="secG"
                     /locus_tag="PLX53_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOY72174.1"
                     /translation="MYTLFSVLIIIVSILLILIVLIQNPKGGGLASNFSGSNQVMGVR
                     KTADFLEKSTWTLAVALILFTLFATLAMPKKSAEEQKSVIESKVKNTAEPNALPTFPV
                     NPTEGNMGSDTTK"
     gene            1761..2039
                     /locus_tag="PLX53_03495"
     CDS             1761..2039
                     /locus_tag="PLX53_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOY72175.1"
                     /translation="MSEVKIKPLADRVLVEPMEAEEKTASGIYIPDTAKEKPQKGTVI
                     AVGPGTKDVTMEVKKGDVVLYGKYAGTEISVDGKDYLMMKQSDILAII"
     gene            2080..>3277
                     /gene="groL"
                     /locus_tag="PLX53_03500"
     CDS             2080..>3277
                     /gene="groL"
                     /locus_tag="PLX53_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654643.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOY72176.1"
                     /translation="MSAKVLNFDIEARDKLKRGVDQLANAVKVTLGPKGRNVVMEKKF
                     GSQQVTKDGVSVAKEIELEDPFENVGAQMVKEVASKTSDTAGDGTTTATVLAQAIVNV
                     GLKNVTAGANPMDLKRGIDKAVAKVIENLKKQSVEVGDDYLKIEQVATVSANNDTEIG
                     KLIAEAMKKVKKEGVITVEEAKGIETTVEVVEGMQFDRGYISPYFITNPEKMETVLEN
                     PMILITDRKISTMKDLLPVLEPVAQAGRAMLIIAEDIEGEALATLVVNKLRGSLKIAA
                     VKAPGFGDRRKEMLEDIAILTGGVVISEEKGLRLDGAKMDMLGTAEKITIDKDNTTIV
                     NGGGKKDNINKRIAQIRTQIENTTSDYDREKLQERLAKLAGGVAVLYVGAATETEMKE
                     KKDRVDD"
BASE COUNT         1032 a          711 c          776 g          758 t
ORIGIN      
        1 aaaccgcttt atcacccaaa ccagcctgtc aatggttgac gattttggcg atttcgactt
       61 ctccgggcag attacccagt ataacgtttc accaatttca atccaggaca acgaggtagc
      121 agcgcaaaac cgcctcacaa tttctgtcag ggtcaaattt gttaacgcca aggaccccaa
      181 gctcagcttc gataaaacct tttcgcagta cgaaaacttc gacagcaacc agaactttgt
      241 gcaggtcgaa gccgaactgg tccggttgat agtcgagaag ctagtggatg atattttcaa
      301 cgcagcagca gcaaactggt agcgctatgg ataatgaaac cctatacggc ataatggagg
      361 cacgactgaa gcctacaccc gaaaccctta accagctgag ggaaaccatt gatgagttcc
      421 cctactttca agggtgccga gtacttattc tcaaaagtct ttcgcaactg ggaaacgatg
      481 agtttgtggc tcaacttcgg aaaaatgctg ttttgctccc ctcacgacgt tatctttact
      541 ttactctata tccttcaaaa ccactagaag agactgaaaa aatagatggc ggtaatgctg
      601 aagataaaaa aatctccaaa cctacaccta aaagaggacg tccccgtaag gctcctaccg
      661 atgaaaggaa accctcattc atactactcg atgacgaaaa acagccagca ataaccattc
      721 atgttgaggc aagtagtaat accaccgaca ccgaaattct ggaacttgct gagggcgaag
      781 gtgaagtaga ttcccaggca aatggaagag gcttgaatga tagcgaagac cttatagcta
      841 aatttataaa caaccagccc accatttctc gttctacaac cccgggtaat aaggacaccg
      901 gtgagaatga ggatatttct atcagctcgg tggaagaacc ggaggagctg gccagcgaat
      961 cgcttgccca aatctacctc agccagggtt accttgaaaa ggcaattagg gtattcgaaa
     1021 aattaagttt gaaatatccg gaaaaaagta gttactttgc cgacgaaatt cggaagatta
     1081 aggaatcaac aaaataagtt aacattaaca actcctacga tgtacacgct attctcagtg
     1141 cttataatta ttgtttccat cctgctcatc ctgatagtgc tgattcagaa tcctaaggga
     1201 ggcggtctgg cttctaactt ttcggggtca aatcaggtga tgggggtgag gaaaacagcc
     1261 gattttctcg aaaaatcaac ttggacattg gcggttgccc tcatactttt tacacttttc
     1321 gccactctgg ctatgccaaa gaaaagcgca gaagagcaga aatctgttat cgaatcaaag
     1381 gtgaaaaata ctgccgagcc caatgccctt cccaccttcc cggttaaccc aaccgaagga
     1441 aatatgggaa gtgatactac caagtaggca gtaaccctta catgatacta aaaaaaatgc
     1501 cagcatgctt tgctggcatt ttttatccct caaggcgtta gctttcatgc catgttggca
     1561 aattcctggt tgttggtata aattgtcaaa gctttccggc gaagcaggga gctaaaagct
     1621 cgaaaattct gccaaaggct ggaagtattg atcaaatgtg gcagaagaac catgaaaaaa
     1681 agtcagttta ccggtttggc atgtattatg atgcacaccc tctcgtaaaa atcaaaatac
     1741 taacattaaa atatagtgct atgtcagaag taaaaattaa accgttagcc gatagggtgc
     1801 tcgttgagcc catggaagca gaagaaaaaa cagcaagtgg aatttacatt cccgatacgg
     1861 caaaggagaa accccagaag ggaacagtaa tagccgtagg tcccggtaca aaagatgtta
     1921 ccatggaggt aaaaaaaggc gatgtagtgc tatatggcaa gtatgccggc acggaaatct
     1981 ccgttgatgg taaggattac ctgatgatga aacaatccga cattctggct attatttaat
     2041 cggtttctac attgttaaac acctaaatca agcataacta tgtcagcaaa agtcttaaat
     2101 tttgatatcg aagcacgcga taagcttaaa agaggcgttg accagctggc caatgccgta
     2161 aaagtaaccc taggacctaa gggacgcaac gtggtaatgg aaaagaaatt tggctcacag
     2221 caggtaacca aggatggcgt ttcggttgcc aaggagattg agctggaaga cccatttgaa
     2281 aatgttggtg cccagatggt taaggaggtg gcatctaaaa cctccgacac agccggcgac
     2341 ggaacaacaa ctgcaacggt tttggcccag gctattgtca acgtaggatt aaaaaatgtt
     2401 accgctggag ccaatcctat ggatttgaag cgtggtattg acaaggctgt tgccaaggtg
     2461 attgagaatc ttaaaaagca atctgttgag gtaggcgacg actaccttaa aattgagcag
     2521 gtggccactg tttcggcaaa caacgacacc gaaattggta agctgattgc cgaagccatg
     2581 aaaaaggtta aaaaggaagg cgtaatcacc gttgaggagg ctaagggtat cgaaactact
     2641 gttgaggtgg ttgagggtat gcagttcgac aggggataca tttcacccta cttcattacc
     2701 aaccccgaga agatggaaac ggttttggag aaccccatga ttcttatcac cgaccgtaag
     2761 atttcgacca tgaaggacct gctacccgtt cttgaaccgg ttgctcaggc aggacgtgca
     2821 atgctcatca ttgctgagga tattgaaggc gaagcacttg caacactggt cgtaaataag
     2881 cttcgcggtt cgctcaaaat tgctgcagta aaggcacccg gatttggcga tcgccgtaag
     2941 gagatgctcg aggatattgc aatcctaacc ggtggtgtgg tgattagcga ggaaaaaggc
     3001 ctacggcttg atggcgctaa gatggacatg ctgggaaccg ccgaaaaaat tacaattgat
     3061 aaggacaaca caactattgt gaatggcggg ggcaaaaagg acaacatcaa caagcgtatt
     3121 gcccaaattc gcacccagat tgaaaacacc acctccgact acgatcgtga gaagcttcag
     3181 gagcgtcttg ccaagctggc aggcggggta gccgttctct acgttggcgc tgccaccgaa
     3241 accgaaatga aggagaagaa ggatagggtt gacgatg
//
LOCUS       DAOOQX010000158         3268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_35139_length_3268_cov_2.651416, whole genome shotgun sequence.
ACCESSION   DAOOQX010000158 DAOOQX010000000
VERSION     DAOOQX010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35139_length_3268_cov_2.651416"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /gene="lipA"
                     /locus_tag="PLX53_03505"
     CDS             <1..787
                     /gene="lipA"
                     /locus_tag="PLX53_03505"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOY72177.1"
                     /translation="LNLATVCEEAKCPNIGECWGNGVATFMILGDICTRACKFCATTT
                     GRPLPPNPNEPEKIARSIQLMALNYCVITSVDRDDLPDQGAAHWRNTVNAIRRVNPNT
                     MIEVLIPDFDGRSDLIDIFLESNPNVVGHNLETVERLTPEVRTKARYRTSLETLSYIA
                     SRGFTTKSGIMLGIGETFDEVVQTLDDLSAVGCRMITIGQYLQPRPNNLPVQRYVTPE
                     EFEEYARIARQKGFVFAEAGPMVRSSYHADKAQKLFGTRADSV"
     gene            827..1564
                     /gene="lipB"
                     /locus_tag="PLX53_03510"
     CDS             827..1564
                     /gene="lipB"
                     /locus_tag="PLX53_03510"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOY72178.1"
                     /translation="MQNVIFTDLGTIDYKEAWDLQEKLFDEVSLNKAEGDGNLNFLLF
                     CEHPHVYTLGKSGQRSNLLVSDEMLKRINASFYHINRGGDITYHGPGQLVAYPIFNLE
                     AFGLSLKSYISLIEQVVIDCLGDFGVPANRLDGATGVWLDAGIKGKERKICAIGVRAS
                     RFVTMHGLAFNINTNLDYFGYINPCGFTDKGVTSLQRELGEEVNPEMVKQRILDHFAR
                     KFNFNVNTRLFPNLKLATNYEGGKHNG"
     gene            1539..>3268
                     /gene="recJ"
                     /locus_tag="PLX53_03515"
     CDS             1539..>3268
                     /gene="recJ"
                     /locus_tag="PLX53_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOY72179.1"
                     /translation="MREGSTMDKRWVFKDVDGDEAVNKLAAELNIPHTLASLLVQRGI
                     KTFDEAKRFFRPSLDDLYDPFLMKDMKEAVDRIVTAIHHGESILFYGDYDVDGTTAVA
                     LLYSFFRQRYSKIGYYIPDRYGEGYGISYKGIDYAFENGYSLIIALDCGIKANEKVLY
                     ASQRKIDFIICDHHFPGDQVPGAVAVLDPQRPDCHYPYKELSGCGVGFKLLQGYCLKT
                     GTPMQELFQHIDLVAVSIASDIVPITDENRVLAYYGLEKINSNPSKGLKTIIQIAGID
                     KQKIAIDDIVFRIGPRINAAGRMESGKSAVDLLCAADDGTAYEMGSLVNSCNNDRKNV
                     DRSITHEALRMIAADARLQHCHSTVLFNPSWHKGVVGIVASRLIETYYRPTVVLTQSG
                     DLATGSARSVPGYDLYQAVEACSDLLENFGGHMYAAGLSLKLENVENFKNRFEEYVSQ
                     TITPELMMPQIEIDARIKLSDINDKFYRILKQFQPFGPGNMSPVFAAENVRDNGECRL
                     VGPDKEHLKLSLAEEGNPNIYHGIAFQLGEYYRDIHNGKSFNVCFNIYENVYRGITTL
                     QLRVKDLKFI"
BASE COUNT          875 a          775 c          793 g          825 t
ORIGIN      
        1 gctcaattta gcgacggtct gtgaagaagc aaagtgtcca aacatcggcg aatgctgggg
       61 aaacggtgtg gcaacattta tgatacttgg tgatatttgc acccgtgcat gcaaattctg
      121 cgctaccacc accggaagac cccttccacc taatccaaat gaaccagaga aaattgcccg
      181 ctccattcag ctaatggcgc tgaactactg cgttatcacc tcggttgacc gagacgattt
      241 gcccgatcag ggagccgctc actggcgtaa cacggtgaat gctattcgta gggtgaaccc
      301 aaataccatg atagaagttc ttatccccga tttcgacggg aggagcgatc ttattgatat
      361 tttcctggaa tcaaacccca acgttgttgg gcataatctt gaaacagttg agcggcttac
      421 ccctgaggta agaacaaaag cccgatacag aaccagcctg gaaaccctta gctacattgc
      481 cagcaggggt tttacaacca aatcgggaat aatgctgggt ataggcgaaa cgttcgatga
      541 ggtagttcaa accctcgacg atctttccgc tgtgggctgc agaatgataa ccattggcca
      601 gtaccttcaa cctcggccga acaacctgcc cgttcagcgt tatgttactc ccgaagagtt
      661 tgaggagtac gccagaatag cccgccaaaa agggtttgta tttgccgaag ccggacctat
      721 ggttcgttcc tcctaccatg ccgataaggc acagaagctg tttggaactc gagctgatag
      781 tgtttaaatg ctttacccac catgagaaaa agaaaaaata catataatgc aaaacgtaat
      841 ttttacggat cttggaacaa tagattacaa ggaagcatgg gatctacagg agaaactatt
      901 cgatgaggta agcctaaaca aggcggaggg cgatggcaac ctgaacttcc tgctgttctg
      961 cgaacatccg catgtttaca cccttggaaa aagtggccaa aggagcaacc tcctggtctc
     1021 cgacgagatg ctaaagcgta tcaacgcctc cttttaccat atcaacaggg gcggagacat
     1081 cacctaccat ggccccggcc agctggttgc ctacccaatt tttaaccttg aggcttttgg
     1141 cctctcgttg aaatcataca tcagccttat tgagcaggtg gttatagact gcctcggcga
     1201 ttttggagta ccggcaaacc gtcttgatgg agctaccggt gtttggcttg atgccggcat
     1261 taagggcaag gagaggaaga tttgcgccat cggtgtccgg gccagccgtt ttgttaccat
     1321 gcatgggctt gctttcaaca tcaatacaaa cctcgactat tttggataca tcaacccatg
     1381 cggctttacc gataaaggcg ttacatcgtt acagcgggaa cttggagaag aggtgaatcc
     1441 tgaaatggtg aaacagcgaa ttttagacca ttttgccaga aagtttaatt ttaatgtaaa
     1501 cacacggctt tttccgaacc tgaagctagc tacgaactat gagggaggga agcacaatgg
     1561 ataaacgttg ggttttcaag gatgttgacg gcgatgaagc cgttaacaag cttgctgctg
     1621 agctgaatat tcctcacaca ctcgcctcgc tacttgttca gcgtggaata aagacatttg
     1681 atgaggccaa acggtttttc agaccatctc ttgacgatct ttacgatcct tttcttatga
     1741 aggatatgaa ggaggctgtt gaccgtatag ttacagcaat tcatcacgga gagtcaatcc
     1801 ttttttatgg cgactacgat gtagatggca caaccgctgt tgccctgttg tattcttttt
     1861 ttcgccagcg ttactctaaa attggctact acattcccga caggtatggc gagggttatg
     1921 gaatatcata caagggcatt gattacgctt ttgagaatgg ttacagccta attatagcac
     1981 ttgactgcgg aattaaggcc aacgaaaagg ttctatacgc cagccagagg aaaatagatt
     2041 ttatcatatg cgaccatcat ttccctggcg atcaggtgcc tggtgcggtt gcagttcttg
     2101 acccgcaacg gcccgattgc cactacccct ataaggaact ttcgggttgt ggggtaggtt
     2161 ttaagctgct tcagggatac tgccttaaaa ccggtacccc catgcaggaa cttttccagc
     2221 atatagacct ggtggctgtt agcattgcct ccgacattgt tcctatcacc gacgaaaata
     2281 gggttttagc atactatggc cttgaaaaaa tcaactccaa cccatccaag ggccttaaaa
     2341 caatcattca gattgccgga attgataaac agaaaatagc cattgacgat attgtttttc
     2401 gaataggccc tcgtattaac gctgccggcc gcatggaatc gggcaaatcg gctgttgatt
     2461 tgctttgcgc tgccgatgac ggaaccgcat acgaaatggg atcgttggta aactcgtgta
     2521 ataacgaccg aaagaatgtt gaccgaagca tcacccacga agcgctcagg atgattgctg
     2581 ccgatgccag gctccagcac tgccattcaa cggttctctt caacccttcg tggcataagg
     2641 gtgtggtggg tatagttgcc tcgaggttga ttgaaaccta ctacaggcct acagttgtgc
     2701 ttacgcaatc gggtgatttg gcaaccgggt cggcacggtc ggtaccgggc tacgatctct
     2761 accaggcggt agaggcctgc tccgacttgc tcgaaaactt tggtggccac atgtacgctg
     2821 ccgggttgtc gcttaagctt gaaaatgtcg agaactttaa aaaccgtttc gaggagtacg
     2881 taagccaaac catcaccccg gagcttatga tgccccaaat tgagattgac gccagaatca
     2941 agctgagcga cattaacgat aagttctacc gcatacttaa gcagtttcag ccttttggcc
     3001 ccggaaacat gtcgcctgtt tttgctgctg aaaatgtgag ggataacggc gaatgccggc
     3061 tggtgggtcc cgataaggaa caccttaaac tttcgctggc tgaggagggc aatcctaata
     3121 tctaccacgg cattgccttc cagctgggcg agtactaccg cgatatccac aatggaaaat
     3181 cgttcaacgt ctgcttcaac atttacgaaa atgtttacag gggcattacc accctgcagc
     3241 ttagggttaa ggacttgaag tttatcga
//
LOCUS       DAOOQX010000159         3267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_35153_length_3267_cov_2.822852, whole genome shotgun sequence.
ACCESSION   DAOOQX010000159 DAOOQX010000000
VERSION     DAOOQX010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35153_length_3267_cov_2.822852"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLX53_03520"
     CDS             <1..553
                     /locus_tag="PLX53_03520"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOY72180.1"
                     /translation="VRDIKPIITTEFNIEGYPVESFIELVLSVKPQQVTLVPDPPDAI
                     TSSSGWDTINNKDLLKRMVATFKREGIRTSIFVNADPEMVKHAADTGTDRVELYTEPY
                     ASMFKVNPEKAIEPFITAADAAKQVGLGLNAGHDLNLENLSYFNRHIPNLLEVSIGHA
                     LISDALYYGLENTIQLYLQQLRS"
     gene            553..1356
                     /locus_tag="PLX53_03525"
     CDS             553..1356
                     /locus_tag="PLX53_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOY72181.1"
                     /translation="MKLFYRELGKGFPLVILHGLYGSSDNWMGIAKRLSASHRIILPD
                     QRNHGQSPHSSSHTYPDMANDLAELVDNLGIDNFHLLGHSMGGRTAMLYQSLFPYMVK
                     SLVVADIAPWPYQPNDEVFRLSLAEHSSIIKGMLSLPLTSIKSRYEAEKMLTGSIPSQ
                     KLRQFLLKNLKRDASGSYSWCLNLPVLESSLRELITGVNLECGKKSPTRVLFVKGENS
                     NYLPENSEVRLTDYYPDCRLVTIRNATHWLHAEQPEAFTKSILEFLQKG"
     gene            complement(1353..2075)
                     /locus_tag="PLX53_03530"
     CDS             complement(1353..2075)
                     /locus_tag="PLX53_03530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOY72182.1"
                     /translation="MLKDVSPENILFLDIETVPAYPAYTDLPEADRKLWDKKAAQLVK
                     DHETAADIYGRAGIYAEFGKIICISTGLVVSKVEERFFRIKSFFGIDEKILLADFAEM
                     LSRFEKRKDVNLCAHNGKEFDFPYIARRMLVNDIRIPDILDVSGKKPWEVRFLDTMEL
                     WKFGDNKHYTSLNLLTHIFGILSPKDDIDGSQVAECYYLQNDLNRIATYCEKDTLAVA
                     QLFMRYKGLPQIAPENVERVNG"
     gene            complement(2078..2734)
                     /gene="udk"
                     /locus_tag="PLX53_03535"
     CDS             complement(2078..2734)
                     /gene="udk"
                     /locus_tag="PLX53_03535"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOY72183.1"
                     /translation="MNSDDESNSFHSITIGIAGGTGSGKTTFSQELVSRLNTDKIVYI
                     SHDSYYKDLSFMPLEERKKMNFDHPDSIEFELLVEHVKLLKEGKAVEQPIYSYLTCTR
                     ARETITIKPRHVVIIEGILIFTCPELRNLLDIKVFVDADADDRLSRVIARDIVERGRS
                     VNKVLDRYEKTVKPMHLQFIEPTKRYADIIVPQGGNNAVAISILASIIEKTLRNHNLV
                     "
     gene            complement(2731..>3267)
                     /locus_tag="PLX53_03540"
     CDS             complement(2731..>3267)
                     /locus_tag="PLX53_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOY72184.1"
                     /translation="NILFNNKNIWPKWDLGVLDREGIIQQAILVEPSLKDEIIKYMEN
                     WDNYFYAVPHNVTAMYRIKETGTKVYVLSNFSQETFEKIREHNAFLFDFDGMVISYQH
                     HLIKPDPRIYQLLIETYNINPARAVFIDDLEENVKAGREAGFFGIQLPPCADVTPYFI
                     FPEGQETVENSEFRQESK"
BASE COUNT          922 a          762 c          712 g          871 t
ORIGIN      
        1 tgtcagggac ataaagccaa tcatcactac agagttcaac attgaaggct atcccgttga
       61 gtcgttcata gagctggtac tcagcgtaaa gccccaacag gtaaccctag ttcccgatcc
      121 gcccgatgct ataacctcca gctccggttg ggataccata aataataagg atttattgaa
      181 acgaatggta gcaactttta aacgggaggg aatacgaact tccatctttg ttaatgccga
      241 tccggaaatg gtgaagcatg ctgctgatac cggaacagac agggtagaac tctacaccga
      301 accctacgca tccatgttta aagtaaaccc tgaaaaagcc atagagccat ttattactgc
      361 agccgatgct gccaagcagg tgggattagg gcttaatgcc ggccatgatt tgaatcttga
      421 gaacctaagc tactttaaca ggcatatacc caacctgctt gaggtttcga ttggtcatgc
      481 cctcattagc gatgcgctat actacggact cgaaaacacc attcagctat acctgcaaca
      541 gcttcgcagc tgatgaaact tttttaccgg gaactaggca aaggttttcc gctcgtcata
      601 ctacacgggc tttatggttc gtccgacaac tggatgggaa tagccaaaag gttaagcgca
      661 agccatagga taatccttcc cgaccaacgt aaccatgggc aaagtccaca ttccagcagc
      721 catacatacc cggatatggc gaacgattta gccgagctgg ttgacaattt gggaattgac
      781 aactttcacc tgctggggca ctccatggga ggccgaaccg ccatgctcta ccagtcgttg
      841 tttccctaca tggtaaaatc gctagttgtt gctgatatag ctccttggcc ataccagcca
      901 aacgacgagg ttttcaggtt gtcgcttgca gagcattcat ctataattaa gggtatgcta
      961 agccttccgc ttacatcaat aaagtcgcgc tacgaggccg aaaaaatgct cacaggtagc
     1021 attcccagcc aaaagttgag gcagtttcta ctaaaaaact taaaaagaga cgcttcgggg
     1081 agctacagct ggtgccttaa cctaccggtt ctcgaaagta gtttaaggga gctgataacg
     1141 ggggtaaatc tggagtgcgg taagaaatcc ccaacccgag tgctattcgt aaagggagaa
     1201 aactcaaact atttaccgga gaatagcgaa gttcggctaa ccgattacta ccctgattgc
     1261 cgcttggtaa ctattagaaa tgcaacccat tggcttcatg ccgaacaacc cgaggcgttc
     1321 acaaaaagca tattggaatt tctgcagaaa ggctaaccat ttaccctctc cacgttttcg
     1381 ggggcaatct gtggtaaccc tttataccgc atgaaaagct gggcaacagc aagggtatcc
     1441 ttctcgcagt aagtggctat ccggttcaga tcgttctgaa gatagtaaca ctcggcaacc
     1501 tgactgccgt caatatcatc ctttggtgaa agaattccaa agatatgggt taaaaggtta
     1561 agcgacgtgt agtgcttgtt gtcgccaaac ttccacagct ccatggtatc aagaaaccta
     1621 acctcccatg gttttttgcc cgacacgtcg agtatgtcgg gaattctaat gtcgttaaca
     1681 agcatccgac gggctatata gggaaaatca aattccttac cgttgtgggc gcataggtta
     1741 acatccttac gcttttcaaa ccggctaagc atttcggcaa aatcggcgag taggattttc
     1801 tcatcgatac caaaaaatga tttaatccta aagaatcgct cctctacttt tgagacgacc
     1861 agaccagttg atatgcaaat aatttttcca aactcggcat aaattccggc ccttccgtaa
     1921 atatcggctg cagtttcatg atccttaacc agctgagctg cctttttatc ccagagttta
     1981 cggtcagctt cgggcaaatc ggtataggcc ggataggccg gtaccgtttc gatgtcgagg
     2041 aaaagaatat tttccggaga aacatcttta agcatggcta cacaagatta tgattcctaa
     2101 gagtcttctc gataatggaa gcaagaatgc taatagcaac tgcattattc cctccctgtg
     2161 gcacaatgat atcggcatac cgcttggtgg gctcaataaa ctgaagatgc atgggtttaa
     2221 ctgttttctc gtaccgatcg agcaccttgt ttaccgagcg cccacgctcc acaatatccc
     2281 gtgcaataac acgcgaaagg cgatcgtcgg catcggcatc aacaaaaacc ttaatatcca
     2341 gcaggttgcg aagttcgggg caggtaaaaa tcaatatacc ctcaatgatt accacgtggc
     2401 gtggtttaat ggtgatggtt tccctggctc tggtgcaggt taggtaggag taaatgggct
     2461 gctcaactgc cttgccctcc tttagcaatt ttacatgttc taccagcagt tcaaactcaa
     2521 tggaatcggg atgatcaaaa ttcattttct tacgctcttc cagaggcatg aacgaaagat
     2581 ctttgtagta ggagtcgtgg gaaatataaa caattttatc ggtgttcagt ctggagacca
     2641 gttcctgtga aaaagtggtt ttccccgaac cggtcccacc ggcaattccg atcgtgattg
     2701 agtgaaaaga atttgattcg tcatccgaat tcattttgat tcctgtctga actccgaatt
     2761 ttccaccgtt tcttgtcctt ccggaaatat aaagtagggt gtgacatcgg cgcatggcgg
     2821 cagttgaata ccgaaaaatc ccgcttctct tccggctttt acattttctt ccagatcatc
     2881 aatgaagacc gcccgagccg gattgatgtt atacgtttcg attaataatt gataaattct
     2941 cggatccggt ttaatcaaat gatgctgata agagatcacc ataccgtcaa agtcgaacag
     3001 aaatgcattg tgttctctga tcttctcaaa agtttcctga gaaaaattgg aaagaacata
     3061 gactttggtg ccggtctctt taatccgata catcgctgtc acattatggg gaacggcata
     3121 aaaataattg tcccagtttt ccatatattt tatgatctca tctttgagag aaggttcaac
     3181 cagaatggcc tgttgaataa ttccctctct atccaggacg cccaaatccc acttcggcca
     3241 tatattttta ttattgaaaa ggatgtt
//
LOCUS       DAOOQX010000160         3254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_35332_length_3254_cov_3.880275, whole genome shotgun sequence.
ACCESSION   DAOOQX010000160 DAOOQX010000000
VERSION     DAOOQX010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35332_length_3254_cov_3.880275"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PLX53_03545"
     CDS             complement(<1..601)
                     /locus_tag="PLX53_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOY72185.1"
                     /translation="MHQLLKVSKQLREDVVRSLASAGSGHLGASLGLADIFTVLYWQV
                     LHHRPLQPGWSERDRLILSIGHAAPVLYAALANSGYFPREELLTLRKLGSRLQGHPGR
                     DHGLPGLELSAGSLGQGLSVAVGLALAAKIDRAPWRVFSIHGDGELQEGSIWEAAMSA
                     AHHKLNNLIALVDRNDCQIDGRTSEVMGLEPLRAKWESFG"
     gene            complement(621..1082)
                     /locus_tag="PLX53_03550"
     CDS             complement(621..1082)
                     /locus_tag="PLX53_03550"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOY72186.1"
                     /translation="MRSLPALLFNSLFLICVFAAEHVHGQGVSGIVQNQHEGNEQGKV
                     KITQDPVVSNLLELHILQNAKNPGMNGFRIRIFLELGQNARRNSEAVMNAFMEKYPDI
                     RVYQSYDNPYWKISVGDFRTRESAQKLYHQLLVDYPKAFIIPLWINFTPLE"
     gene            complement(1121..1648)
                     /locus_tag="PLX53_03555"
     CDS             complement(1121..1648)
                     /locus_tag="PLX53_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOY72187.1"
                     /translation="MKVKRFLAAFVPIFMVLMSGCAPKFQDIKINGINKVEYNGLKND
                     VVKLNLAVGIENPNSKRITVKEVEFKSWIKQRELGTVRVKNRIEIPPNSSQIYSIPVE
                     VKLNSSADALKLMISGEKILTMITVEGFVKGSSFPIVKKIKIPRQPLNNLINKYQNGF
                     IITDTISVRNSTVGN"
     gene            complement(1768..2514)
                     /locus_tag="PLX53_03560"
     CDS             complement(1768..2514)
                     /locus_tag="PLX53_03560"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOY72188.1"
                     /translation="MSGHNKWSTIKRKKGALDAKRSKEFSRIIKEITVAVKESGSDPD
                     GNPRLRLAINNAKGVNMPKDNIMRAISKAEKDPENFHELTFEGYGPGGIALFIECLTD
                     NNNRTVSAIRSILTKRGGSLGTNGSLSFLFDRKGIFTIAKDKLKLDEVELDLIDAGAQ
                     SIDDNGDFYVVTTEMEDFGRMNKKLEELNVEVENASLQRIPNDYKTLDKDSSLRFLKL
                     IDELEDNDDVQNVYHNLEMTDEVVAAMENE"
     gene            2694..>3254
                     /locus_tag="PLX53_03565"
     CDS             2694..>3254
                     /locus_tag="PLX53_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776123.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOY72189.1"
                     /translation="MDFNGEEPNIFEGISTAGTGNGAKVYLETYGCQMNVNDSEVVAS
                     ILLANGYSITQEINEADIILINTCSIRENAETRVFGRIDLFGQVKKNKPSVIIGVLGC
                     MAERLKDELLEKKKIVDLVVGPDAYRELPTLLRAAESGQKGVNIMLSADETYADISPV
                     RLDKYGVSAFVSIMRGCNNMCSYCVVP"
BASE COUNT          902 a          740 c          703 g          909 t
ORIGIN      
        1 agccaaaaga ttcccatttt gccctaaggg gctcaagccc cataacctcc gatgttcggc
       61 catcaatctg gcagtcgttt ctatcaacca gggcaatcag gttattgagt ttatgatggg
      121 cagcgctcat ggctgcctcc cagattgatc cctcctgtag ttccccatcg ccatggatgg
      181 aaaagacccg ccatggggct ctatcaattt tagcggccag agctaagcct actgctaccg
      241 aaagtccctg accaagcgaa cctgcagaaa gttcaagtcc gggcaatcca tgatcccttc
      301 cgggatgtcc ttgtaggcgc gagcccagct ttcgtaaggt gagaagttcc tccctgggaa
      361 agtatcccga gttggccaaa gcggcgtaga gcaccggggc agcatgtcct atagatagta
      421 tcagcctgtc ccgctcgctc caaccgggct gaagcggacg gtgatgcaga acttgccagt
      481 agagcactgt aaaaatatcg gcaagcccta acgaggctcc gagatggcct gaaccagctg
      541 aggctaaact ccgaacgaca tcctcccgaa gttgtttcga tacctttaga agctggtgca
      601 agtttgcctg gaccataaca ctactccagt ggtgtaaagt taatccatag cggaattata
      661 aatgctttag gataatctac aagaagctgg tggtaaagtt tttgagcgct ctcccgagta
      721 cggaaatcac ccaccgaaat tttccagtag ggattgtcgt agctttggta aactctaata
      781 tcagggtact tttccataaa agcattcatc actgcctcgg agttgcgacg agcgttttgc
      841 cccagctcca ggaatatgcg aatacgaaaa ccgttcatcc ccggattctt agcattctga
      901 agtatgtgca gctccaacag gtttgaaacc actggatcct gggtaatttt aacctttccc
      961 tgctcattgc cttcgtgctg attttgcaca attccactta ccccctgtcc gtgaacatgc
     1021 tcagcggcaa atacacaaat cagaaaaagc gagttaaaga gaagtgctgg taaactacgc
     1081 atcggaatat aattgcacca caaaagtagc aaaagttagc ctaattgcca accgttgaat
     1141 tgcggacgga aattgtgtcg gtaattataa acccgttttg gtacttgttg atgagattgt
     1201 tcagaggctg ccgggggatt ttaatttttt ttacaatggg gaagctcgac cccttaacaa
     1261 aaccctcaac ggttatcatt gtcagaatct tttcgccgga aatcatcaac tttagggcat
     1321 cagcagaaga atttaatttt acttcaacag ggatagagta gatttggctt gagttgggtg
     1381 gaatttcaat tctattttta actctaacag ttcccagttc cctttgtttt atccacgatt
     1441 taaactcaac ctcttttaca gttatccttt tggaatttgg attctcaatc cctactgcta
     1501 aattcagctt aacaacgtca ttctttaacc cgttatactc aactttgttt atcccgttga
     1561 ttttaatgtc ctgaaatttg ggtgcgcatc cggacattaa taccatgaag ataggcacaa
     1621 aagcagcaag aaacctttta accttcatct attgtagaat tttaatttaa ttagcaataa
     1681 ctgttccatg ctgcttataa aaagaagaaa aagccatcga acaagctagc cgatggcctc
     1741 aatattgtaa aacgtttata aattctacta ctcattttcc atagcagcca caacctcatc
     1801 ggtcatttcc aggttatggt aaacattttg aacatcgtcg ttatcctcaa gctcgtctat
     1861 cagcttaagg aatctaagcg atgaatcctt gtcaagtgtt ttatagtcat tggggatacg
     1921 ctgtaggctt gcattttcaa cttcaacatt cagctcctcc agctttttgt tcatccggcc
     1981 aaagtcttcc atttcggtag ttactacgta gaaatctcca ttgtcatcga tactttgggc
     2041 gccggcatct atcaggtcaa gctccacctc gtcgagcttc agcttatcct tggcaatagt
     2101 aaaaatgccc ttgcggtcga aaagaaacga taatgagccg ttagttccaa ggcttccacc
     2161 acgcttggtg agaatggaac gaatagcact aaccgttcga ttgttattat cggtaagaca
     2221 ttctataaat aatgctattc cacccggacc ataaccctca aaggtaagct catgaaagtt
     2281 ttcgggatcc ttctcagcct tggatattgc ccgcataatg ttatccttgg gcatgttcac
     2341 cccctttgcg ttgtttatag caaggcgcag ccgggggtta ccatcaggat cggagccact
     2401 ttccttgacc gccacggtaa tttccttgat tattcgggaa aactccttgg agcgtttggc
     2461 atcaagcgct cccttttttc ttttaatggt tgaccattta ttatgtcctg acataaagta
     2521 tttgtttagc ttattcaatt ataccatgca aaagtaaccg cttgtttttt gctatcaaaa
     2581 aaaatcggaa gttttcacac tggttcaggt aaaaaatata ggtttaggac ttgcaggact
     2641 aataaaagta tatctgaatt tttagcattt cattacattt gtaaaaaaaa agaatggact
     2701 ttaacggcga ggagcccaac atttttgaag gtatatcaac cgcaggaaca ggcaacggcg
     2761 caaaggttta cctggaaaca tacggttgcc agatgaatgt aaacgatagc gaggtagttg
     2821 cctccattct gctggcaaac ggatactcca taacgcagga gataaacgaa gcggacatca
     2881 tacttatcaa tacctgttca attagggaaa atgccgaaac cagggttttt ggccgtattg
     2941 acctgtttgg acaggtaaag aaaaataaac catcggtaat tataggcgtt ttaggatgta
     3001 tggccgagcg gcttaaggat gagctgctgg agaagaagaa aattgtggat ttggtggttg
     3061 gcccggacgc ctatagggag ctgccaactc tgctacgagc ggctgaaagt ggacaaaagg
     3121 gggtaaacat tatgctctca gccgatgaaa cctatgcaga cataagcccg gtgaggctcg
     3181 acaagtatgg cgtatcagcc tttgtatcca taatgcgggg gtgcaacaac atgtgctcct
     3241 actgtgtggt tccc
//
LOCUS       DAOOQX010000161         3211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_35956_length_3211_cov_3.127060, whole genome shotgun sequence.
ACCESSION   DAOOQX010000161 DAOOQX010000000
VERSION     DAOOQX010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35956_length_3211_cov_3.127060"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLX53_03570"
     CDS             <1..351
                     /locus_tag="PLX53_03570"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HOY72190.1"
                     /translation="YVEYAAKKLIFDYMGLYATAPQRVIVSILVVYSLYSLSYALIML
                     MNIGIVEATGPSGAHLGLVAKGFYFSVVTFFTIGYGDFAPDGFARIIAGTEGFMGVFM
                     MSYFTVAFVRKILR"
     gene            complement(373..1050)
                     /gene="trmD"
                     /locus_tag="PLX53_03575"
     CDS             complement(373..1050)
                     /gene="trmD"
                     /locus_tag="PLX53_03575"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOY72191.1"
                     /translation="MRIDIITVLPELLESPLGHSIIRRAQKKGLVEIYLHQLRDYTTD
                     KHRTTDDYAFGPDAGMVLKVEPIFKVIEKLKGERTYDEVIYTSPDGEPYTQGLANQLS
                     TMQNIILLCGHYKGVDHRVREHLVTREISIGDYVLSGGELAAAVIVDSVVRLVPGVLT
                     DETSALTDSFQDGLLAPPVYTRPAEFNGWKVPEILISGNFKEIEKWQLEQSIERTRRL
                     RPNLLGE"
     gene            1334..1783
                     /locus_tag="PLX53_03580"
     CDS             1334..1783
                     /locus_tag="PLX53_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOY72192.1"
                     /translation="MTLVTFNSPLTSGRPSGTSVVESIFDALRDTAMNLAEVSASYPA
                     VNIFEEPERFRIEVAAPGYKKEQFNIQVDANDVLTLSVNEGKNNVEGESYLRREFANG
                     SFERHFTLGEMIDANKIEATYRDGILTIVIAKKEEAKPQKPRTISIS"
     gene            2015..>3211
                     /locus_tag="PLX53_03585"
     CDS             2015..>3211
                     /locus_tag="PLX53_03585"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOY72193.1"
                     /translation="MSLENNNEITMSTNDLTDPKDQNLANSEGTMDSSSLAEQENEPV
                     ALERIEISNQLGDDPQGIHFDASTTVKSEGESAEATNIPVNEIELPAESEELEEVEDI
                     EDGDEEVDEESEEIKIDSVDFSSLTKDEMVQTLKKLINEKPIQKIKAEVESIKSIFYR
                     KHKLEVERLRREFVEAGGDVAGFTPPEDDAEIQIKELLKLYREKRAQYNGELEVQKKQ
                     NLELKMQIIEQIKDLVNSSEAVNVTFQEFRNLQQKFREVGPVPQANLNDLWENYHHHV
                     QNFYDYIKINKELRDLDLKKNLEEKIKLCEKAEELLLEPSVVAAFKKLQKYHTQWREV
                     GPVPHENQNEIWERFKEATAKINKKHQEYFESLRDEQKRNLDAKAALCEKAEELASLV
                     IKTNKEW"
BASE COUNT          969 a          711 c          734 g          797 t
ORIGIN      
        1 tatgttgagt atgccgcaaa aaagttaata ttcgattaca tggggctata tgccactgct
       61 cctcagcggg ttatcgtaag cattctggtt gtatattcgc tttacagcct atcctatgcg
      121 ctgataatgc tcatgaacat aggaattgtt gaggcaacag gtccttcggg tgcccacctt
      181 gggttggttg caaaaggttt ctacttcagc gttgttactt tcttcaccat tggctatggc
      241 gattttgctc ccgatggatt tgcacgaatt atcgccggca ccgaagggtt tatgggggtt
      301 tttatgatgt cgtactttac ggtggctttt gttaggaaaa ttcttaggta gctaaaacga
      361 ctaaaacaat attcactcac caagtaagtt tggacgaagc ctacgggttc gctcgatgga
      421 ttgttccagc tgccactttt caatctcctt aaagtttccg gaaataagaa tttcgggcac
      481 tttccaaccg ttaaattcgg caggccgggt gtaaaccgga ggtgcaagca gcccatcctg
      541 aaacgaatcg gttaaagcag aggtttcgtc ggttagcaca cctggcacaa gcctcaccac
      601 gctatctaca ataaccgcag cagccagctc acctcccgaa agcacgtagt cgccaattga
      661 aatttcccgc gtaaccaaat gctccctaac gcggtggtca actcccttat agtgcccaca
      721 aagtagaatt atgttctgca tggtgcttag ctggttggct agtccctgcg tatagggttc
      781 gccatcgggc gatgtgtaga taacctcatc gtaggtacgt tcgcccttta gcttctcaat
      841 aaccttaaat attggctcca ccttcagcac catccctgca tcgggtccaa aggcgtaatc
      901 atcggtagtt cgatgcttat cggtggtgta gtcccttagc tggtgaaggt atatctccac
      961 caaccccttt ttctgcgccc ttcttattat cgaatgccct aaaggacttt cgagaagttc
     1021 gggcaaaacg gtaattatat ctattcgcat ggctgccttt ttacaaccgc aaatttacgg
     1081 ttttttaccg tgtaatgcta ccccagggca aggcacgttg tagccttacc acggcattcc
     1141 aaatccggta gtaaaactgt tctctgtttc tcaacagtaa gctaaccatc agcaaaacca
     1201 caccatgacg cgcaaaacgc cattatgaca tattcggact tcattatacg tcaatatggc
     1261 cgattgcttc gtttggcata aagttggaag tagattaaga aacgttaaat caacacaata
     1321 aaacagaaaa gctatgacac tggtaacttt taacagtcca ttaacctctg ggaggccaag
     1381 tggaacatcg gtagttgaaa gcatattcga cgcccttaga gatacggcca tgaacctggc
     1441 agaagtgagc gccagctatc cggcagtgaa tattttcgag gaacccgaga ggttccgtat
     1501 tgaggttgct gcaccaggct ataaaaagga acagttcaat atccaggttg acgctaacga
     1561 cgtgcttacc cttagcgtga atgaggggaa aaacaatgtt gaaggagaat cgtacttgcg
     1621 tcgtgagttt gccaatggtt catttgaacg ccattttacg ctaggcgaaa tgattgatgc
     1681 aaataaaatt gaagccacgt accgtgatgg catcctgacc atcgtcatcg ccaaaaagga
     1741 agaggcaaaa ccccaaaaac ccagaaccat aagcatcagc taaccgcaag ttgatacaaa
     1801 tagcaacaca taaacctaat ggcgttttct ccattaggtt tttttgttcc tactttccct
     1861 actgcattgg gaagcatttt gcataaaagt caacctgtaa ttttatacca attttgggta
     1921 atcctcaaaa atcaatactt ttgacgaaaa taatttgagg atgggatgaa ttgcatattt
     1981 tcgcaaatca cgttaaaccc agccatacgt ttgtatgtct ttagaaaata ataacgagat
     2041 taccatgagc accaacgatc ttactgatcc taaggatcaa aaccttgcaa atagcgaggg
     2101 tacgatggat tcttccagtt tagccgagca ggaaaacgag cctgttgcgc tagagcgtat
     2161 cgagatttcc aatcagctgg gggatgatcc ccagggtatc catttcgatg caagtacaac
     2221 cgtaaaatcc gaaggggagt ctgctgaagc aaccaacatc cccgttaatg agattgaact
     2281 acccgccgaa agcgaggagc tggaagaggt agaagatatc gaagatggtg atgaagaggt
     2341 tgatgaggaa agtgaggaaa ttaaaattga cagtgtagat ttttcgagcc tcacaaagga
     2401 tgagatggtt cagaccctca aaaaactcat aaacgaaaag cccatccaaa aaatcaaggc
     2461 tgaggttgaa agcataaaga gcatattcta ccgtaagcat aagctggagg ttgagcgttt
     2521 acgacgcgag tttgttgaag ccggcggtga tgtggcgggt tttacaccac cggaagatga
     2581 tgcggaaatt caaataaaag aactgctcaa gctataccgc gaaaagcggg cacaatacaa
     2641 cggcgagctg gaggttcaga agaaacagaa ccttgaactt aagatgcaaa ttattgagca
     2701 aataaaggat ttagtgaaca gctccgaagc ggttaacgtt acctttcagg agtttagaaa
     2761 tcttcagcaa aagttccggg aagttgggcc cgttccgcaa gcaaacctga acgatctgtg
     2821 ggaaaattac caccatcatg tgcaaaactt ctacgactac ataaaaataa acaaggagct
     2881 acgcgacctt gacctaaaaa agaacctcga ggagaagatt aagctttgcg aaaaagccga
     2941 ggagctcctg ctcgaaccct cggttgtagc agctttcaaa aagctgcaaa agtaccatac
     3001 ccagtggcgg gaagttggcc cggttccaca tgaaaatcag aacgagattt gggaaaggtt
     3061 taaggaggca accgctaaaa ttaacaagaa gcatcaggag tacttcgaat cgctcaggga
     3121 tgagcaaaaa agaaaccttg acgccaaagc tgccctttgc gaaaaggccg aagaacttgc
     3181 ctcgctggtt atcaaaacca acaaggagtg g
//
LOCUS       DAOOQX010000162         3210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_35979_length_3210_cov_2.584786, whole genome shotgun sequence.
ACCESSION   DAOOQX010000162 DAOOQX010000000
VERSION     DAOOQX010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35979_length_3210_cov_2.584786"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLX53_03590"
     CDS             <1..311
                     /locus_tag="PLX53_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOY72194.1"
                     /translation="HKLCIDVSFTDEYPASVCDKTLTHTLEAIAHEASLEVNFLEQPN
                     RWSEDFAHFSTCFPSVIFGLGAGENHPDIHTPEYDFPDELIDNAVDFLGKIANKLMEG
                     "
     gene            645..1571
                     /locus_tag="PLX53_03595"
     CDS             645..1571
                     /locus_tag="PLX53_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOY72195.1"
                     /translation="MKSFWKRDLGLVPSEEWEPYCWVDVEIPTEEERKYLFDELGVPE
                     AFFNDIEDVDERPRIEFEDEWFFILLRMPYKNVDVSLPFTTIPVGIIFKDDIFVSISF
                     YRTEVIPDFIQFTIRKGIVIKDHFDLVLRLMLSSSVWFLKYLKQINLDIRLAEDQLEK
                     SIKNEDLQTLLSIEKCLVYFTTSLKGNDILLHRIKNLRNYKDTYNPDLIEDVEIELRQ
                     AQETTSVHSDILSGMMDAYASVISNNLNNVMRQLTSISIILMIPTLIASIYGMNVPNG
                     LENNPWGFGLVVLASFLFSILGIVLFRRKKWF"
     gene            complement(1682..2689)
                     /locus_tag="PLX53_03600"
     CDS             complement(1682..2689)
                     /locus_tag="PLX53_03600"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HOY72196.1"
                     /translation="MLLKFFRRFRISTLLVMLGVITLFWAGTLMQKYPPSTFTFDSFG
                     MPLYVLIVKILSPYSFLSKLVAFALTLTVAIYLLQFNVRHTVVRQRTYLPFLFFVLVA
                     SSLTPLQRLNPAIIANIFILVSLSHVISTYQNSEALDHLFRSGLLLGIGTLLYAPTVV
                     FVFLIFWSIATLRKFNLREWLVTLVGILVPWGAVVFYFYWFDLGASEPFIQLANSLCL
                     EAESSADKIAPIILCVVVGVPLLVAILHMLTGMGSQKIAIRKHQNIMLLFTVVALLSF
                     FVVPSCSYEILYIAAAPISLLLSEYFSDAKGKYWPEILFLIFILGLVSMQLYPLLMHS
                     L"
     gene            complement(2706..>3210)
                     /locus_tag="PLX53_03605"
     CDS             complement(2706..>3210)
                     /locus_tag="PLX53_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HOY72197.1"
                     /translation="SGLASEGIPYCGFIFFGLMNVVGNPFVVEYNARLGDPETQVVLP
                     RIKSDMLELLMLCAAGKMNEAELVVAPQFAATVILASGGYPGSYEKGKAISGLEASAN
                     AIIFHAGTTSSGSMTLTAGGRVLACTGTGESMEDALASSYCAANAITFESKYFRSDIG
                     MDVYPKS"
BASE COUNT          995 a          714 c          705 g          796 t
ORIGIN      
        1 agcataagct gtgtatagac gtttcgttta ccgatgagta ccccgcctcg gtgtgtgata
       61 aaacgctaac ccacaccctt gaggcaattg cgcatgaagc tagtctcgag gtgaatttcc
      121 tcgagcaacc caacaggtgg agcgaggatt ttgcacattt ctccacctgc ttcccttcgg
      181 tgatttttgg ccttggtgcc ggagaaaacc atcctgatat acacaccccc gagtacgatt
      241 tccccgatga gctaatagac aatgcggttg acttcttggg aaaaatagca aacaagctaa
      301 tggaaggtta agcttaaacc gatgccggtg caaaacttgc aagccaccat gttcaaacct
      361 tttggaaaaa gtagtgcatg caataatttc attgccattt tcagtttgat tttgcggtta
      421 aaaaaccatt atagcttccc tgataacctg ctaccatcac tccataaatg aaataagcac
      481 gttgaaagaa aatataggtt tttaacaaat cttctttgcc attaaaatta tatctttgcg
      541 ccgttcaata atttgaatat atggacgatt atcaccctga ccgttaaacc cgaatggagg
      601 aacatgtact gagactccat tcatttaaac atggaggtta gtacatgaaa tcattctgga
      661 aaagagatct tgggcttgtt cccagcgaag aatgggagcc ctactgctgg gtggatgtcg
      721 aaattccaac cgaagaggag cgaaaatacc ttttcgacga gctgggagtt ccggaagctt
      781 tctttaacga tatcgaggac gtggacgagc gtccacgtat agaatttgag gatgagtggt
      841 ttttcatcct gctacgaatg ccctataaaa atgtagatgt aagccttcct tttaccacta
      901 ttcccgttgg tataattttt aaggatgaca tttttgtaag catttccttt taccgaacag
      961 aggttatccc cgatttcatt cagttcacca tccgtaaggg cattgtcata aaagaccact
     1021 tcgacctggt gctcaggctt atgctctcat caagcgtatg gtttctgaag taccttaaac
     1081 aaataaacct cgacatcagg ctcgccgaag accaactcga aaaatctatt aagaacgagg
     1141 acttgcaaac gctgctgagc attgagaagt gcctggttta cttcaccacc tccttaaagg
     1201 ggaatgatat tttgctacac cgcattaaaa atttaaggaa ctataaggat acatataacc
     1261 ccgatcttat agaagatgta gagattgaac tacggcaagc tcaggaaaca acaagtgtgc
     1321 attcggatat tcttagcggt atgatggatg cctacgcttc ggttatatca aacaacctga
     1381 acaacgtgat gaggcagcta acctccattt caattattct catgattcct acattgattg
     1441 ccagcatcta cggaatgaat gtgccaaacg ggctggaaaa taatccttgg ggctttgggt
     1501 tggtggttct tgcatcattc cttttctcca ttctgggaat tgtcctcttt cgcaggaaaa
     1561 aatggtttta gcagctgcca gggtcaaaat cagcttataa accaaacttg ctgcaagttg
     1621 tagcaacagt cgttttgggt aaataccctg tagagtcgta aaaaaaatca aacctcaccg
     1681 gctagaggct gtgcatcagt aaggggtaaa gttgcattga tactaaacct aaaataaaaa
     1741 tcaggaataa gatttccggc cagtacttac cctttgcgtc ggaaaaatac tcggaaagca
     1801 acaggctaat gggagctgct gcaatgtata atatttcgta tgagcaggac ggcacaacaa
     1861 agaaggaaag caaggcaaca acagtaaata aaagcataat attctgatgc ttcctgatgg
     1921 caattttctg acttcccatg ccggttaaca tgtgtagaat tgcaaccagc aacggaacgc
     1981 caacaactac acacagaata atcggggcaa ttttgtccgc agaactttcg gcttcaaggc
     2041 aaaggctgtt tgccagctgt atgaaaggtt cggatgcacc taaatcaaac cagtagaagt
     2101 agaaaacaac agctccccac ggaaccaaaa tgcccacaag ggtaaccaac cattcacgca
     2161 ggttaaactt cctcagggtg gcaatgctcc agaaaatcag gaaaacaaat actaccgtag
     2221 gggcatacag tagagttcct atacctagca gtagcccgga gcgaaaaagg tggtcgagcg
     2281 cttcagaatt ctgataggtt gagataacat ggctaagtga aacgagtata aaaatattag
     2341 caatgatagc agggtttaaa cgttgaagcg gtgttaagga cgaggcaacc aggacgaaaa
     2401 aaaggaaagg aaggtaggta cgctgccgaa caaccgtatg ccttacgtta aactggagca
     2461 ggtaaatagc aacggtaagg gtaagagcaa aggcaaccag tttagacaga aaactatacg
     2521 gcgataaaat tttgacaata agcacataaa gcggcattcc aaagctatcg aaagtaaaag
     2581 tggaaggcgg gtacttctgc ataagcgttc cggcccaaaa aagcgttatt actcccagca
     2641 tgacaagcag agtagaaatc ctaaaccttc tgaagaactt gagcagcatc tggttagcgt
     2701 gttagctatg attttgggta aacatccata cctatatcgc ttcggaagta cttgctttcg
     2761 aaggtaattg cattggcagc acagtatgat gaggccagag catcttccat gctttctcct
     2821 gtaccggtac aggccagcac cctacctcct gcggttaacg tcatggaacc ggagctagtg
     2881 gtaccagcat gaaaaataat tgcgttggca gaagcctcta aaccactaat agctttcccc
     2941 ttttcgtagc tacccgggta tcctcccgag gcaaggatta ccgtagcggc aaattgggga
     3001 gctacaacca gctcggcctc gttcattttc ccggcagcac aaagcatgag cagctcaagc
     3061 atgtcgcttt tgatacgagg caaaacaacc tgggtttccg gatcgcccaa gcgggcattg
     3121 tactccacca cgaacggatt ccccaccaca ttcatcaacc cgaaaaaaat aaagccacag
     3181 tagggtattc cctcgctagc taacccgcta
//
LOCUS       DAOOQX010000163         3190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_36261_length_3190_cov_3.934609, whole genome shotgun sequence.
ACCESSION   DAOOQX010000163 DAOOQX010000000
VERSION     DAOOQX010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36261_length_3190_cov_3.934609"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PLX53_03610"
     CDS             <1..187
                     /locus_tag="PLX53_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72198.1"
                     /translation="VEKVEMLSELLYRDGLFTSNDVLRKDLLCKALALIERIEKQSKS
                     FSVERNDRKVGILAMLN"
     gene            complement(254..1597)
                     /locus_tag="PLX53_03615"
     CDS             complement(254..1597)
                     /locus_tag="PLX53_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOY72199.1"
                     /translation="MAKILVVDDDSTFCLMLKTFLTKHEHSVVEAFSFDEAVRQIRSE
                     NFDVVLTDFRLPEKSGMDVLSEAKHKSSATVVIMMTGYADIRMAVNAIKHGAYDYVTK
                     PINPDEILTSISRALPGNRKAIDKDGTHNFIFINGNSEHSQEVSRYISIVAPTNLSVI
                     IQGDSGTGKEYIARKIHAESKRSSKAFVPIDCGALPRELAASEFFGHLKGSFTGAIAD
                     KIGQFEVANGGTLFLDEIGNLPYDIQVNLLRAIQERKIKRIGSSKEIPVDVRIIVASN
                     ENLLNMVNSGAFREDLYHRLNEFTIRVAPLHERKDDLLIFAKHFLSLANVELGKGING
                     FDADVLDILMDYTWPGNLRELKNVIRRAVLMEQSNLLTVSSLPDEIVHRSGPGDDDKS
                     AKHVENLRNIKEKAEYDLIISTLEKAKYNKSKAALLLNIDRKTLYNKMKHYGISG"
     gene            1708..3132
                     /locus_tag="PLX53_03620"
     CDS             1708..3132
                     /locus_tag="PLX53_03620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY72200.1"
                     /translation="MQQQKVSRGLVLALVMVSSFITPFIGASVNLALPLIGKDFGLNA
                     VGMSWVTMAFLLSSAVFLVPIGKIADIAGRKRVFFWGNIIMAISSVLCAISPSGAVLI
                     TFRAVQGLGSAMVFATGVALITSVFPPSDRGKAIGLNVTSVYIGLSAAPLIGGLLISW
                     FGWESLFYVPAFLGIFSALSIRMAVRSEWADARGENFDFAGAAIYVPSMTLFMYGFSQ
                     LPDPVAVCLTILGFIGLIFFVRVELRASYPVFNVRLFRNNRLFAMSNVAALINYATTF
                     AITFVLSLYLQYIKGLSPREAGLLLIVQPLLMAFVSSWSGKMSDKHDPRILASLGMGI
                     IAVGLALLIPIGKNTSITYLIVAQAILGFGFGLFSSPNTNSVMGSVEKKFLGIASGTV
                     ATMRLTGQMASMGIATLILQLYIGKSDINPGNYRQFGWSVGTTFTVFVLLCIVGVWAS
                     LARGKNNGNSMRELNPENEMERIA"
BASE COUNT          764 a          695 c          777 g          954 t
ORIGIN      
        1 cgttgaaaag gttgaaatgc tttcggagct tctttaccgc gatgggttgt ttactagcaa
       61 tgacgtttta cggaaggatc tgctatgtaa ggcgcttgcc ctaattgagc ggattgaaaa
      121 gcaaagtaag tctttttcgg ttgaacgaaa cgataggaaa gtgggaatac tggccatgct
      181 gaactagaaa gaatgttgaa atgtgccaac aggaggcaga ctgaatggca gcatttttta
      241 ctatatcatt tttttagcca gaaattccgt aatgcttcat cttgttgtac agggttttac
      301 ggtcaatatt tagcaatagg gcagccttgc tcttgttgta cttagccttt tcgagggttg
      361 atataatcaa atcatactcg gctttttcct ttatgttacg gaggttttca acgtgcttgg
      421 ccgatttatc atcatcacca ggaccggaac gatgtacgat ttcgtcggga agacttgaaa
      481 cggttagcaa gttcgactgc tccatcagca cagccctacg aatcacattc ttaagctccc
      541 ttagatttcc tggccaggtg tagtccatta gaatatccag aacatcggcg tcaaacccgt
      601 tgataccttt tcccagctca acgttggcaa ggctaagaaa atgcttggca aaaattagca
      661 ggtcgtcttt tcgctcatgc aggggggcaa cccgaatggt gaactcgttt aaccggtggt
      721 acaagtcctc cctgaatgct ccggagttca ccatatttag aaggttttcg ttggaggcaa
      781 caattattct aacatccacg gggatttcct tgctgctccc aatacgctta atttttcgtt
      841 cctggatggc cctaagaaga ttaacctgga tgtcgtaggg taggtttccg atttcgtcga
      901 ggaaaagggt tccaccattg gctacctcaa actgacctat tttatcggca atggcgccgg
      961 tgaaggagcc tttcaggtgt ccaaaaaact cgctggcggc aagctcgcgc gggagagccc
     1021 cgcagtcaat cggaacaaaa gctttgctgc tacgcttgct ctcggcatga atcttacgtg
     1081 ctatgtactc ctttccggtt ccgctatcac cctgaataat aacggaaagg tttgttggag
     1141 ccacaatgct tatataccgg cttacctcct ggctatgctc gctattgcca tttataaata
     1201 taaagttgtg agtgccgtcc ttgtcaatgg ctttcctgtt gcccggtaga gccctgctaa
     1261 tggaggtaag aatctcatcg ggatttatag gtttggtaac atagtcgtag gcaccgtgct
     1321 ttatggcgtt aaccgccatg cgaatatcgg catacccggt catcatgatt acaactgtgg
     1381 cggatgattt atgcttggcc tcggaaagca catccattcc gcttttctct ggaaggcgga
     1441 agtcggtcag gacgacatca aaattctccg agcgaatttg cctaacagcc tcgtcaaacg
     1501 aaaatgcttc caccacgcta tgctcatgct tggttagaaa ggtcttgagc atcaggcaga
     1561 atgttgagtc atcgtccact accagaatct tagccataag tcgtgaagat ctaatccaac
     1621 gctaaaatag ctggtttttc agctaaaaaa aaataaacac tactttgaag agtactaaaa
     1681 cgaatacctt tgcgaaaatt ttttaagatg cagcaacaga aggttagtag gggtttagtt
     1741 ctggcgttgg ttatggtatc gtcgtttatt actccgttta ttggtgcttc ggttaatctg
     1801 gctctaccgc ttatcggtaa ggattttggt ttgaacgcag tggggatgag ctgggttact
     1861 atggcatttt tgctctcctc tgcggtgttt cttgttccga taggtaagat agccgatatt
     1921 gcggggcgaa aacgtgtatt tttttggggc aatataatca tggcaattag ttcggtgctg
     1981 tgcgctatct ccccatcagg tgccgtgctc ataactttta gggctgttca ggggttagga
     2041 agcgccatgg tttttgctac aggggtggca ttaattacct cggttttccc cccatcggac
     2101 cgtggaaagg ctattgggtt gaatgttacc tcggtgtata ttgggctatc ggcggcaccc
     2161 ctaattggtg gcttactaat ttcctggttt ggatgggaaa gtttatttta tgtaccggct
     2221 tttctgggaa ttttttcagc gctttccata aggatggcag ttcgatcgga gtgggctgat
     2281 gccaggggtg agaatttcga ctttgcgggg gctgcaatct acgttccctc catgaccctt
     2341 ttcatgtatg gcttttcaca acttccggac cctgttgcag tttgcctgac cattctaggc
     2401 tttatcgggc ttattttctt tgttagagtt gagcttcggg ctagctaccc tgtattcaac
     2461 gtcagacttt ttcggaacaa ccgcttgttt gccatgtcaa atgtagccgc actaatcaac
     2521 tatgcaacca catttgccat tacatttgtg ctcagccttt accttcagta tattaagggt
     2581 ctatctcctc gcgaggcggg attgctgcta attgttcagc cgctactaat ggcatttgta
     2641 tcgtcgtggt ctggaaaaat gtccgataag cacgatccgc gaattctggc atcattagga
     2701 atgggcataa tagccgtagg gctggccttg ctaattccaa ttggtaagaa taccagcatt
     2761 acctacctaa ttgttgcaca agccatactg ggattcggct ttggtctttt ttcatcaccc
     2821 aacaccaact ctgttatggg ttctgtagaa aagaagtttc tgggaattgc ttcggggaca
     2881 gttgccacca tgaggttaac cggccagatg gcaagcatgg gcattgccac ccttatactt
     2941 caactttaca ttggcaagtc ggacatcaac ccaggaaact acaggcagtt tggctggtcg
     3001 gtggggacaa cctttacagt ttttgttttg ctatgcattg ttggggtgtg ggcatcgctt
     3061 gccaggggga aaaacaacgg caactccatg cgggagttaa atcccgaaaa cgagatggaa
     3121 agaatagcct aggaatggtt tgagttttac ccggatgatt gggtgttttc aaaccattcc
     3181 taggctattc
//
LOCUS       DAOOQX010000164         3189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_36282_length_3189_cov_2.814614, whole genome shotgun sequence.
ACCESSION   DAOOQX010000164 DAOOQX010000000
VERSION     DAOOQX010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36282_length_3189_cov_2.814614"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..734
                     /locus_tag="PLX53_03625"
     CDS             267..734
                     /locus_tag="PLX53_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOY72201.1"
                     /translation="METTLKEIISISGYSGLFKIVSQGRKGVIVESIADGNRMTAPIT
                     SKVTALTDISVFTNSTDLPLIEVLKLIRNLEDSKPTLNPKTSTDKAIREYFEKVLPDF
                     DRERVYTSDIKKILSWYNQLQENNLLAILDVEEAKPEEPVAEEHSNTAQDAES"
     gene            complement(803..2797)
                     /locus_tag="PLX53_03630"
     CDS             complement(803..2797)
                     /locus_tag="PLX53_03630"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOY72202.1"
                     /translation="MKRCLVVSLIVLLAASAKGQNKEEDIFNLSLEELLNLEVSISTK
                     TATNIRNTPGILTVITSEDIKNSGVNGIIDLFQLFVPGFDFGVDVEGVVGMGVRGIWA
                     HEGKVLFMVNGLELNDGMFASVPFGNHFPLNNIERIEVIRGPGSAMYGGFAGMGVVNI
                     ITRDVYKPGGYVSYTANHTGRQFSQNNVGFGTGVHQNYLNIGISGNYGNGSRSDRDYV
                     DYHGQSRSMRNASDTYTRQLDLKLNYKNFNLVGFIDDYSYQQIDLWDSYYPGPPLTES
                     FKSSLVQATYDFKPNNNLTITPKISYKWQQPWKLTALGAGYGYNNNKTFDGLLGGIST
                     SYTKNKLTIVGGAELKHDHLNQPELVDTVYEETFRNGKEQLSYTNTAIYGLLQHDSRV
                     GNFTLGARYDFSSEYGNSFVPRVGIAKVFDFMHFKVMVSQSFRVPGGILPNRLPQGNP
                     EIKPEKGTSYEVAIGFKMPFQSYITFNIFDVSFDKVIVYNLGNDDITGVGYYKNEGTI
                     GTRGIEADFRHISTKISSFATFAYYMRKHSKTDSLYRVPDRESYFLGFSPLRLNGGFN
                     FRVNNRLSLTLSGSYFGKRYAFTQREGNGMDILEKLNPLFMLGCNVNIKNIVLKGLTA
                     TIEVNNMLGESFVFPQPYKGSHAPLPGLDRSFGFNLVYEY"
     gene            complement(2999..>3189)
                     /locus_tag="PLX53_03635"
     CDS             complement(2999..>3189)
                     /locus_tag="PLX53_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72203.1"
                     /translation="LKVHPEGCLVFEDSFSGIQAATAAGMRVVAMATTHTRNELGKNL
                     TVADSFYDVLAFILRENE"
BASE COUNT          895 a          686 c          630 g          978 t
ORIGIN      
        1 tgtttttttt acctttattt acattgctta taacgatatg gtaagcggaa aataccagtg
       61 gccattgtgg ttaaactacc tggttaacat tgctgcaacc ttttacctgg tgtgggcaaa
      121 attccattat ggccggaaaa gcaaccggta aactttcagc tacccagacg ccggctattt
      181 ttgtgtattg ttcatttaaa cggtacgcag tttttaattt aaaatttttt caacttaact
      241 ttacaccgaa aatataacta ggaagtatgg aaactacact taaggaaatc atttctattt
      301 cgggctactc cggtttattc aaaattgtat cgcaagggcg gaaaggggtt attgttgaat
      361 ccattgctga tggaaaccga atgacggccc ccattacctc aaaggttacc gccttaaccg
      421 acatatcggt ttttactaat tccaccgatt taccccttat cgaggttctt aagctaatac
      481 gtaacctcga agattctaaa cccactttaa atcctaaaac ttcgactgat aaggccattc
      541 gggagtattt cgagaaggtt ttacccgatt ttgataggga gagggtttat accagcgata
      601 ttaaaaaaat tcttagctgg tacaaccagc ttcaggaaaa caatctttta gcaattctcg
      661 atgttgaaga agcaaaacct gaagagcctg tagctgaaga gcatagcaac acagcccaag
      721 atgctgaaag ttagcaataa cttttttacc tattgctttt cactatgaaa atggccctag
      781 cagggccatt tttacttaag ttttagtatt catagacaag attaaagcca aaactcctgt
      841 caagtccggg taaaggggca tgtgagccct tatagggctg gggaaataca aacgattccc
      901 ccagcatatt gtttacctca atagttgcag taagaccctt tagcactata ttcttaatgt
      961 ttacattgca gccaagcata aaaagagggt tcagtttttc gagtatatcc attccatttc
     1021 cttccctttg ggtaaaggcg taacgttttc caaagtaaga tcctgacaac gtgagagata
     1081 aacggttgtt taccctgaag ttaaaaccac cgttaagcct taggggcgaa aatcctaaaa
     1141 agtaactttc cctgtccggt accctgtaaa ggctatcggt cttggaatgt ttacgcatgt
     1201 agtaggcgaa tgtagcaaat gatgaaattt tagtgctgat atgcctaaaa tcggcttcaa
     1261 tacctcttgt gccaattgta ccttcatttt tatagtaacc tactcctgtt atatcatcat
     1321 tacccaagtt gtaaacaatt accttatcga aagatacatc gaaaatgtta aaggtaatgt
     1381 aggattgaaa aggcatttta aaccctatag ccacttcgta gctggttcct ttttcaggct
     1441 tgatttccgg attgccttgt ggcaaccggt tgggaaggat tcctccgggg accctaaatg
     1501 actggctcac cattaccttg aagtgcataa aatcaaaaac cttggcaatg cctactcttg
     1561 gtacaaacga gttgccgtac tcgctggaaa aatcgtacct ggctccaaga gtaaagtttc
     1621 ctacacggct atcgtgttgt aagagcccat aaatagccgt atttgtgtag gacagctgct
     1681 ctttgccatt cctaaatgtt tcctcgtaaa ccgtgtcaac cagctcgggc tggttaaggt
     1741 gatcatgttt gagctcggct ccgccaacaa tggtcagctt attttttgta tagcttgttg
     1801 aaattcctcc taaaagccca tcaaatgttt tgttgttgtt atacccatag ccagctccca
     1861 gggcagtgag tttccagggc tgctgccatt tgtaagagat ttttggagta atggtaaggt
     1921 tgttattagg cttaaaatca taagtagctt gaaccagcga gctcttgaag ctttcggtta
     1981 aaggcggtcc tgggtagtag ctatcccaga ggtcgatttg ctggtaggag tagtcatcaa
     2041 taaacccaac caggttgaag tttttgtagt taagcttcag gtcgagctgg cgggtgtagg
     2101 tatcgctagc attcctcatt gaacgtgatt gtccatggta gtcaacgtaa tccctgtcgc
     2161 ttcgcgaacc attaccgtag tttccggata tacctatgtt aaggtagttt tggtgtacgc
     2221 ctgtaccaaa acctacgtta ttttgtgaaa attgcctgcc tgtgtggttg gcagtgtaag
     2281 aaacgtatcc tcccggttta tatacatccc tggtgatgat gttcactaca cccataccag
     2341 caaaacctcc atacattgca gagcccgggc cccttatcac ctcaattcgt tctatattat
     2401 tcaacggaaa gtggttaccg aatgggacgg atgcgaacat cccgtcgttt agctccaacc
     2461 catttaccat aaacagaact ttaccttcat gtgcccaaat accccgtact cccattccta
     2521 ctactccttc aacatcaacc ccaaagtcga aacctggcac aaaaagttga aaaagatcta
     2581 ttattccgtt tacaccgctg ttttttatat cctcactggt gattaccgta agtattcccg
     2641 gtgtatttct aatatttgtt gctgttttag tagatatgct aacctccaag ttcagcagct
     2701 cctccagcga gaggttaaaa atgtcttctt ctttattttg acctttagcc gaagcagcta
     2761 gcaaaacaat taggcttaca actaaacatc ttttcatatg ccaatcatat ttgtttgtta
     2821 aaatgaaggc ttaaaacttc accatttata gccatcgtgg ttgatgtaaa gatatttact
     2881 ttgtgattgc ttatttaata atatattgat aggcaatata gccataattg gagatagtat
     2941 caatggcact ttgaaatttt ggttcattct tccaatctag tggctgcagc tgaaatggct
     3001 actcgttttc ccttaaaatg aaggctagca cgtcatagaa gctatcagct actgtaagat
     3061 ttttaccaag ctcatttcgg gtatgagttg ttgccatggc taccacccgc atccctgccg
     3121 ctgtggccgc ctggattccg gaaaaagaat cttcgaaaac taaacacccc tcagggtgaa
     3181 cctttagta
//
LOCUS       DAOOQX010000165         3187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_36307_length_3187_cov_3.614943, whole genome shotgun sequence.
ACCESSION   DAOOQX010000165 DAOOQX010000000
VERSION     DAOOQX010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36307_length_3187_cov_3.614943"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PLX53_03640"
     CDS             complement(<1..994)
                     /locus_tag="PLX53_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOY72204.1"
                     /translation="MKRLLCIFILAALPAVAYLQPPLLTVAESSNFTSTSTHSEVMAY
                     VERLQKTSKFLRVEKIATTTEGKDLPLLVIGNPVPKDPKDLHGRLVVYIQANIHAGEV
                     EGKEASLMFARDLVAKRDPKLLKHLVVLICPDLNADGNDRISKLNRTYQNGPANGVGE
                     RQNGQWLDLNRDAMKLESPEMRGVVANVLNRWDPAIIVDCHTTNGSYHQEPVTFTWMM
                     NPNGDRELIRYMRDRMMPWVSQTLSGKYGTLNCFYGEFINQQNYEEGWISYASEPRYF
                     SNYVGIRNRLGILNENYVYADYRERVLGCYNLIASITDYALAYQTEIMQMLARAD"
     gene            1207..>3187
                     /locus_tag="PLX53_03645"
     CDS             1207..>3187
                     /locus_tag="PLX53_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOY72205.1"
                     /translation="MRTLSKYKIIPENAMKRNLLTVSLMVLVNTIIFGQNIPIEKANY
                     ELPARFSPKKVEKMVFSTTVQPHWLKNSTRFWYSYETPSGKSYIIVDPAKRSKGFLFD
                     NNNMAAQLSLLTKEPFDAQHLPIQKIKFIKNETAIQFEVKSTLVDEDKSDEDEENDMV
                     KKKEGKAKAKPDKKTYYFEYDLATRKLMLLEDYKKPKENPEWANISPNGEMVVFSRHH
                     NLYWMDKENYEKAKKNEDDSTIIEHQLTTDGEKYYSYGYTRENEDNVDEEKNKDKRKP
                     TYITWSFDSKLFAMTRTDMRNVKELWLVKTLSSPRPTLETYKYHMPGEKEAPKDELLI
                     FNLAQDTIIKPDVAAFKDQDLMILNARVKANERDDDYVATRWLSKYNDRLYFVRTSRD
                     LKRVDVCYVDTKTGKVNVVIEERLNTYIDFNGISLINNEKEIIHWSERDGWGHFYLYD
                     NNGKFIRQITSGPFHCENLVGYDETTKTLFFTANGCEPNEDPYYLHLYRVGLDGNGMK
                     LINPGNFDHYISMDDGNRYFVDNYSRVNTNPETRLLDAQGNELLALEKADLSLLFQAG
                     YKFPEIFKVKAADGITDLFGVMYKPFDFDSTKLYPLIEYVYPGPQTESVNKSFSARLD
                     RTDRLAQFGFIVITVGNRGGHPARSKWYHNYGYGNL"
BASE COUNT          924 a          759 c          734 g          770 t
ORIGIN      
        1 tgtcggccct tgccagcatt tgcatgattt ccgtttggta tgccagagca taatcggtta
       61 tggaggcaat caggttgtag caccccagca cgcgctcccg ataatcggca taaacataat
      121 tctcgttaag tattcccagg cgattcctta ttccaacgta gttggagaag tagcggggtt
      181 cagaggcata agaaatccaa ccttcctcgt agttttgctg gttaatgaac tcaccgtaga
      241 agcagttcag cgtgccgtac ttgccactta gggtttgcga tacccagggc atcatcctat
      301 cgcgcatgta gcgaataagc tccctatcgc cgttgggatt catcatccat gtaaaagtaa
      361 caggttcctg gtgataggag ccattggtgg tgtggcagtc cacaatgata gccggatccc
      421 agcggttaag cacgttggct acaactcccc tcatttcggg cgattccagc ttcatagcat
      481 cacgattcag gtcgagccat tgaccgttct gcctctcgcc taccccgtta gcaggcccat
      541 tctggtacgt tctgttcaac ttgcttattc ggtcgttacc atcggcattc aaatccggac
      601 aaattagcac cactaaatgt ttcagcagct tcgggtcgcg cttggcaacc aggtcgcggg
      661 caaacatcag cgaggcttcc ttgccttcaa cctctccagc gtgaatgttg gcctgaatgt
      721 acaccaccaa acggccatgc aaatccttag gatctttcgg cacagggttt ccaattacta
      781 gcagcggcag gtccttaccc tcggttgttg tggcaatttt ttccacccgt aaaaatttgg
      841 acgttttctg cagcctttca acatatgcca tcacctcgct gtgggtagaa gttgaggtaa
      901 agttgctgct ctcggcaaca gttagtagcg gtggttgtag gtaagccaca gctggcagag
      961 cggctagtat aaaaatgcaa agtaaccttt tcatcagttc ctaatattat tgttagctat
     1021 gtccagaaaa aataaacttt aatgaatttt gagatattta aaaatcattc catccaagca
     1081 tttaaaaatg tagcaggtga gtttacatgt tttgaaactg catccctttg gttacccggt
     1141 ttttaaacac aggaacacgg cttttgaccg tttgcctttt taatggttta acagctaaac
     1201 catgtaatga gaactttatc aaaatataaa attattccgg aaaatgccat gaaaagaaac
     1261 ctgcttaccg tatcattgat ggtgcttgtc aacaccataa tttttgggca aaatatccca
     1321 atagaaaagg ccaactacga gctacccgcc cgcttctcgc caaaaaaagt agagaaaatg
     1381 gttttcagca caactgttca gccacactgg ctgaagaaca gcacacgctt ttggtacagc
     1441 tacgaaacgc caagcggaaa atcgtacatt attgttgacc cggcaaaacg tagcaagggt
     1501 tttctctttg ataacaacaa catggccgca cagctctcgc tgctgactaa ggaaccattt
     1561 gatgcccaac atcttccaat acagaaaatt aagtttatca agaatgaaac agctatccag
     1621 tttgaggtga aaagcacgct ggtggatgag gacaaatcgg atgaggatga ggagaatgac
     1681 atggttaaga aaaaggaggg taaagccaaa gcaaagcccg ataagaagac atactacttt
     1741 gagtatgacc tggctacccg aaagctaatg ctattagagg actacaaaaa acccaaggag
     1801 aatccggagt gggctaatat ttcgcccaac ggtgagatgg tggtgttcag cagacatcac
     1861 aacctatact ggatggataa ggagaactat gagaaggcaa aaaagaacga ggacgactcc
     1921 accattatag agcaccagct taccaccgat ggcgaaaagt actacagcta cggctatacc
     1981 agggaaaacg aggacaacgt ggatgaggag aaaaataagg ataagcgcaa gcccacctac
     2041 attacatggt cgttcgattc caagctgttt gccatgacca gaaccgacat gcgaaatgta
     2101 aaggagctat ggctggttaa aacgctatcg tcgccacggc ccacgctgga aacctacaag
     2161 taccacatgc caggtgagaa agaagcacca aaggatgagc tgttgatatt caatttggct
     2221 caggacacca tcatcaaacc agatgttgcc gcatttaagg atcaggattt aatgattctg
     2281 aatgcccggg taaaggctaa cgaacgcgac gacgactatg ttgccaccag gtggctgtcg
     2341 aagtataatg ataggctcta ctttgttcgc acgtcaaggg acctaaagcg cgttgacgtt
     2401 tgctacgttg acaccaaaac cggcaaggta aatgttgtaa tcgaggagag gctcaacacc
     2461 tacatagatt ttaatggtat aagcctgatt aacaacgaga aggaaattat tcactggtcg
     2521 gagcgcgacg gatgggggca cttctacctc tacgacaaca acggcaaatt catccgacag
     2581 attacctccg gacccttcca ctgcgagaat ttggtgggtt acgatgaaac caccaaaacg
     2641 ttgtttttca cagccaacgg ttgcgaaccc aacgaagacc cctactacct gcacctgtac
     2701 agagttgggc ttgacgggaa cggaatgaag ctcatcaacc caggaaattt tgaccactac
     2761 atcagcatgg atgatggcaa tcgctatttt gttgacaact actcccgggt caacaccaat
     2821 cccgaaacca ggctactcga tgcccagggc aacgagctgc ttgcactgga aaaagccgat
     2881 ttaagcttac tcttccaggc tgggtacaaa ttccccgaaa tattcaaggt aaaggctgcc
     2941 gatggcatta ccgacctgtt cggggtgatg tacaaaccat ttgattttga ctccaccaag
     3001 ctctacccgc tgattgagta tgtttacccg ggaccgcaaa ccgaatcggt taacaaatcg
     3061 ttttctgctc gcctagacag aaccgacagg ttggcgcagt ttgggtttat agtgattacg
     3121 gttggtaaca ggggcggcca tcctgcacgc tccaagtggt accacaacta cggctatggc
     3181 aaccttc
//
LOCUS       DAOOQX010000166         3180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_36426_length_3180_cov_3.536640, whole genome shotgun sequence.
ACCESSION   DAOOQX010000166 DAOOQX010000000
VERSION     DAOOQX010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36426_length_3180_cov_3.536640"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PLX53_03650"
     CDS             <1..887
                     /locus_tag="PLX53_03650"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOY72206.1"
                     /translation="NQRSKEMVELQKVWKTIGFAPKKDNNKVYERFRAACDTFFNNKR
                     DFYSETKEDQLNNLQLKTELCIQAESLKDSTEWKKTTEDFINLQKRWKEIGPVPRKNS
                     DEIWKRFRAACDYFFTQKSSHFSQVDNSYDKNLKDKEMLIAEIAAYQLTDNPEADLLA
                     LKEFQRRWAEVGFVPIKNKEDIQKRYREAINKHFETLNIGEEKRNMFNFKTKLESATG
                     NPRQENKLRQERDKFFNRLRQLEGDIALWENNIGFFTRSKNAEAMIKEVERKIEKAKE
                     EIRILEQKIKMIDSMDSK"
     gene            1094..2707
                     /locus_tag="PLX53_03655"
     CDS             1094..2707
                     /locus_tag="PLX53_03655"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOY72207.1"
                     /translation="MPHNTKYVFVTGGVASSLGKGIISASLGKLLQSRGFSVTIQKLD
                     PYINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLNLPTSQMNNVTTGKIYQTVI
                     SKERHGDYLGKTVQVIPHITDEIKRRIKLLGTKHKLDVVITEIGGTVGDIESLPYIEA
                     VRQLRWELGPTNSAFIHLTLVPYLSASGELKTKPTQHSVKVLLENGIQPDVLVLRTEK
                     PLNDDIRRKVALFCNVDSSAVVESIDVKTIYEVPLLMHKEKLDETIMKKLNLPVQEPP
                     KLDDWNEFINKLKNPKSKVKVGLVGKYVELHDAYKSIVESFIHAGAVNECHVELEMIQ
                     SESITASNVKEKLGHLQGILVAPGFGHRGVEGKIEAIRYARENQVPFFGICLGMQCAV
                     VEFARNVLGLQEANSTEMVRNTPFPVIDLMEEQKGITDMGGTMRLGSYPCQVKKGTKA
                     YMAYHKLETHERHRHRYEFNNQFLPQFEAAGMVAAGINPETNLVEIMEISSHPWFVGV
                     QFHPEYKSTVINPHPLFVEFVKATMNFGG"
     gene            2785..>3180
                     /locus_tag="PLX53_03660"
     CDS             2785..>3180
                     /locus_tag="PLX53_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOY72208.1"
                     /translation="MDRNTILGLVLIFLIMIGFGYLAAPSKEEREAYVRKADSLARVQ
                     AEYEQKRAEEEKAKAAMLADTIQKKEVQSQVGSFAPALEGERKFISLENDLMRVVVSN
                     RGGRIYSVELKKFKTHAGEPLVLFNGDDNE"
BASE COUNT          984 a          698 c          761 g          737 t
ORIGIN      
        1 ggaaccagcg ctcgaaggag atggtggagc tgcaaaaggt ttggaagacc atcggctttg
       61 cgcctaaaaa ggacaacaac aaggtttacg aaaggttcag ggcggcatgc gacacttttt
      121 ttaataataa gcgcgatttt tacagcgaaa ccaaagagga tcagctaaat aacctgcagc
      181 ttaaaaccga actttgcata caggccgaaa gcctgaagga tagcacggag tggaaaaaaa
      241 ccaccgagga ctttatcaac ctgcaaaagc gctggaagga gattggaccc gttccacgta
      301 aaaattcgga tgagatttgg aaacgcttcc gtgcagcttg cgactacttt ttcacccaaa
      361 agtccagcca cttctcccag gttgataaca gctatgataa gaacctgaag gataaggaga
      421 tgcttattgc cgaaattgcc gcttaccagc taaccgacaa tccggaagcc gacctgctgg
      481 ccttaaagga atttcagcgc cgctgggccg aggttggctt tgtgccaata aaaaataagg
      541 aggacattca gaaacgatac cgcgaggcta tcaacaagca cttcgaaacg cttaatattg
      601 gcgaggaaaa gcgcaacatg ttcaacttta aaaccaagct agaaagcgca acaggaaacc
      661 ccagacagga aaataagctc cggcaggagc gcgataagtt cttcaaccgc ctgcgccagc
      721 tggagggtga cattgctcta tgggaaaaca acatcgggtt ctttacccgt tcaaaaaatg
      781 ccgaggcaat gattaaggag gttgaacgta aaattgaaaa agctaaggag gaaattcgga
      841 ttttggaaca aaaaatcaag atgatcgact ctatggacag taagtagcaa aagcggtgca
      901 tttcaaccat gcaaaacagt tattagctgt gaactgtaga agcagttttg gttaaaggta
      961 aaaagtttga aaatgaaatt ttgaatccgc cagtaggcgg acaagttccg cccggaggcg
     1021 gacaagttta ttttattttt tatcctttat ccttgaattt ttctgttaag ctagattaaa
     1081 acaagttcaa gccatgccac acaacaccaa gtatgttttt gttaccggcg gagttgcctc
     1141 atcgctggga aaggggataa tatcagcttc acttgggaag ttgctacaaa gcagaggttt
     1201 ctcggtaacc attcaaaaac tcgatccata catcaacgta gatccgggaa ctctaaaccc
     1261 atacgagcat ggcgagtgct acgtaaccga ggacggggca gaaacagacc ttgacctggg
     1321 acactacgag aggtttttaa acctcccaac atcccagatg aacaacgtta caaccggtaa
     1381 aatttaccaa actgttataa gcaaggagag gcacggcgat tacctgggta aaaccgtcca
     1441 ggttattccc catattaccg atgagataaa gcgaagaata aaactccttg gaacgaagca
     1501 taagctcgat gtggtgataa ccgaaattgg tggaacagta ggcgacattg aatcgctacc
     1561 ttacattgaa gcggtgcgcc aacttaggtg ggagcttggt ccaaccaact ccgcattcat
     1621 ccatcttaca ctggtgccct acctgtcagc ttcgggtgaa cttaaaacaa aacccaccca
     1681 gcattccgtt aaagtgcttc ttgaaaatgg tatccaaccg gatgttctgg tactaagaac
     1741 agagaaaccc ttaaatgatg acatccgtcg caaggtagcg ctattctgca acgttgattc
     1801 ctcggcagtg gttgagtcta ttgatgtaaa aactatttat gaggttccgc tacttatgca
     1861 taaggagaaa ctcgacgaga ccatcatgaa aaaactcaac ctgcctgttc aggaacctcc
     1921 caaacttgac gattggaacg agtttataaa caagcttaaa aatccgaagt cgaaagtaaa
     1981 agttgggttg gttggaaagt acgttgagct acacgatgcc tataaatcaa ttgttgagtc
     2041 gtttattcat gccggagcgg taaacgaatg ccatgttgag ctcgaaatga ttcaatcgga
     2101 aagcatcacc gcatcaaatg taaaagagaa gctgggccac ctgcagggca tactggtagc
     2161 gcccggtttt ggtcacaggg gagttgaggg caaaattgaa gccattcgat atgccagaga
     2221 gaatcaagtc cctttctttg gcatttgcct gggaatgcag tgtgctgtag ttgagtttgc
     2281 cagaaatgtt ctgggcttgc aggaggccaa ctctaccgaa atggtgcgca atacgccctt
     2341 tccggtaatt gatttgatgg aggagcaaaa gggaatcacc gacatgggtg gcaccatgcg
     2401 gcttggctcc tacccctgcc aggtaaaaaa gggaactaaa gcctacatgg cctatcataa
     2461 actagaaacc cacgaaaggc accggcaccg ctacgagttt aacaaccagt ttttgcccca
     2521 gttcgaggca gccggcatgg tcgcagccgg cattaatcca gaaaccaacc tggttgaaat
     2581 aatggaaatt tcttctcatc cgtggttcgt tggggtgcag ttccatccgg agtataaaag
     2641 cacggttatt aacccgcatc cgctttttgt tgaatttgtt aaagccacca tgaattttgg
     2701 tggctaacca tccaaatatt actacctttg ccaccctaaa aggatggctt tttttcactt
     2761 aaacaaccag aaaatcagca gcagatggac aggaatacaa tattaggttt agtgcttatt
     2821 ttcttaatca tgattggctt tgggtacctt gccgcacctt caaaagaaga gcgtgaagcc
     2881 tacgtacgta aagccgattc gttagccagg gtgcaagccg agtatgaaca gaaaagagcc
     2941 gaagaggaaa aggctaaggc tgccatgctt gctgacacca ttcagaaaaa ggaagttcag
     3001 tcccaggtag gctcatttgc ccctgccctg gaaggtgaac gcaagttcat aagcctggaa
     3061 aacgacctca tgcgcgtggt ggtgtcgaat aggggtggca gaatatactc cgttgagcta
     3121 aaaaagttta aaacccatgc cggtgagcca ctggtacttt ttaacggcga tgacaacgaa
//
LOCUS       DAOOQX010000167         3175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_36506_length_3175_cov_2.896154, whole genome shotgun sequence.
ACCESSION   DAOOQX010000167 DAOOQX010000000
VERSION     DAOOQX010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36506_length_3175_cov_2.896154"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PLX53_03665"
     CDS             <1..517
                     /locus_tag="PLX53_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOY72209.1"
                     /translation="EALASRASATEFTSDELSLQDISDLLWAANGINRPEIGKKTAPS
                     ATNSQDIDVYMVTSKGAYIYEPVQHQLKLVNAGDFRELVAGRQKNFANAPLFCIMVSD
                     ISRFRGNDDSQKMRWAAMDAAIVSQNINIFCSGVGLRTRTRAFMEADKLREALKLTPS
                     QFPMLNNPVSN"
     gene            740..1096
                     /locus_tag="PLX53_03670"
     CDS             740..1096
                     /locus_tag="PLX53_03670"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HOY72210.1"
                     /translation="MFRFVKILLTLVAVLLAEITFAQKFFEVEKVEDAQVKFYAVDDP
                     KDADLLFCIIYEEKLITKAGIMMEVETPREAQVLIIFVDDPALADIKVWLVEKPEEVV
                     WKNESKRKLLSVGGLK"
     gene            1174..2343
                     /locus_tag="PLX53_03675"
     CDS             1174..2343
                     /locus_tag="PLX53_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOY72211.1"
                     /translation="MNDQHRYNQRGVSASKEDVHNAIKNLDKGLYPKAFCKVVPDILA
                     GNPDYCLVMHADGAGTKSSLAYAYWRETNDMSVWKGIAQDAVVMNLDDLLCVGVTDNI
                     LLSSTIGRNKNLIPGEVISAIVEGTAQFCEEMQKYGIHITLTGGETADVGDLVRTVIV
                     DSTVIARLKRAEVISNSNIQDGDVIIGLSSCGKANYEQEYNGGMGSNGLTSARHDVFS
                     SYLADKYPETFDPSVPRELLYSGSFKLTQMVDEVGITAGKLMLSPTRTYAPVVKEVLD
                     SYRPVIHGLVHCSGGAQAKVLNFVDNLHVVKDNLFPIPPLFRIIQSESNTSWREMYKV
                     FNMGHRFEIYVFPEFADDIIAISKDFGVDARVVGYCSSFQGKKLTIKSEKGEFYY"
     gene            2593..>3175
                     /locus_tag="PLX53_03680"
     CDS             2593..>3175
                     /locus_tag="PLX53_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HOY72212.1"
                     /translation="MSDNTITRKLEGVRQKFEEIGQKITDPAVMADMKKFVSLNKEYK
                     ALEPLVAAYEKYKNILSNIESSRQILSSEKDEELREMAKEELDALTQEQEKLEEEIKL
                     LLLPADPEDSKNAVMEIRAGTGGDEASIFAGDLFRMYTKFFERKGWKYEVTSSSEGTV
                     GGYKEIVMSVTGNGVYGILKYESGVHRVQRVPQT"
BASE COUNT          900 a          631 c          776 g          868 t
ORIGIN      
        1 ggaggcccta gccagcagag cttccgccac cgaatttaca tccgatgagt tatccctgca
       61 ggatatttcc gatttgcttt gggctgcaaa cggcattaac cgacccgaaa ttgggaaaaa
      121 aacggcacct tctgcaacaa actctcagga tatagacgtt tatatggtta cttcaaaagg
      181 agcctatatc tatgaacctg tacagcatca gctaaagctg gttaatgctg gagattttag
      241 ggagttagtg gctggccgtc agaagaactt tgccaacgct ccgctattct gtattatggt
      301 ttcggatatc tcacgattcc ggggtaatga tgattctcag aaaatgaggt gggctgcaat
      361 ggatgccgcc attgtttcgc aaaacattaa catattttgc tctggggtgg gcttgagaac
      421 aagaaccagg gcttttatgg aagcagataa gctacgtgag gcgctaaaac ttaccccctc
      481 acagttccca atgctaaata atccggtgag caattaggca tattgtagag ttcctaacgc
      541 aattttgctt gtttaccatc tggaatttta taatactgac attactgaac cctgatgtgc
      601 gggaaacttt cagcagataa aaacttatca tcaaatcagc aacaagaaca agttatgttt
      661 tgagcacttg ctgcaacttt tttaattttg agcagggtaa aagtttacac tatacatggt
      721 taaatttgca agcagttcta tgtttcgttt cgtaaagata cttctaaccc tggttgctgt
      781 tttactggct gagattactt ttgcccagaa attctttgag gtcgaaaaag ttgaggatgc
      841 ccaggtaaaa ttttatgcgg ttgatgaccc caaggatgcc gatctgctgt tctgcattat
      901 ctatgaggaa aaattgatta ctaaggccgg cattatgatg gaggtggaaa cgcccaggga
      961 ggcgcaagtg ctcattattt ttgttgacga tccggctctt gccgacatta aggtttggct
     1021 ggttgaaaaa ccggaggagg tggtgtggaa aaacgaatcg aaacgaaagt tgctttctgt
     1081 tggtgggctc aagtaatgat gggggctact tttttttaat ttgaagtaat agcggtgcag
     1141 gtaatctcga accataaact atcaatttcc tatatgaacg atcaacaccg atacaaccaa
     1201 cggggagttt cagcatcaaa ggaggatgtt cataacgcaa taaaaaatct ggataagggc
     1261 ctatacccta aggctttttg taaggttgtt cccgatattt tagctggcaa tccggattac
     1321 tgcctggtga tgcatgccga tggggcagga acaaagtcgt cgttggccta tgcatactgg
     1381 cgcgagacta atgatatgtc ggtttggaaa ggaatagctc aggatgctgt tgtgatgaat
     1441 ttagacgacc tgctttgtgt gggcgttacg gataacatac ttctctcctc tactattggc
     1501 cggaataaga accttattcc cggcgaagtc atttctgcga tagttgaggg aaccgctcaa
     1561 ttttgcgagg agatgcaaaa atatggtatc cacataacac ttacgggtgg agaaacagcc
     1621 gacgtaggcg acttggtcag aaccgtaatt gttgactcta ccgttattgc caggctgaag
     1681 cgagcggagg taatatctaa cagtaacatt caggatggag atgttatcat cggcctatca
     1741 tcgtgtggca aggctaatta tgaacaggag tacaacggag gcatgggtag caacgggctc
     1801 acctctgccc gccatgatgt ttttagcagc tacctggccg ataagtatcc cgaaacattc
     1861 gacccgtcag ttcctcgcga gttgctgtac agtggtagct ttaagctcac ccagatggtt
     1921 gatgaagtcg gaattactgc cggaaagctg atgctttcac ctacaagaac ctatgcccct
     1981 gttgtgaaag aggttcttga cagctaccgt cctgtgattc atggccttgt gcattgctcg
     2041 ggaggagctc aagctaaagt gctcaacttc gttgataacc tacacgtggt taaggataac
     2101 ctttttccca ttcctccttt gtttaggatt atccaaagcg agtcgaatac atcgtggagg
     2161 gaaatgtaca aagttttcaa catgggtcac cgatttgaaa tatacgtttt ccctgaattt
     2221 gccgatgata ttattgccat ttctaaggat tttggcgtag atgcaagggt tgttggctac
     2281 tgtagcagct ttcaggggaa aaagctaaca attaaatcag aaaaaggtga attttactac
     2341 tagaaagtgg tgtttgagct cttttgaacg tgaagaataa aatttttgtt tagccctctc
     2401 cgtgcttgct gttattagtc attcaatcag ctgtttacca cattattaaa aaggtgtatt
     2461 ataccctgca ccaaagatct gaacgtcaca gtaatatcaa ccttgcacct tgttgggtaa
     2521 agcattatga gtggtggaag aatactcgct ttctaaattt atgcttacct ttgcgcatta
     2581 tattaccagt ctatgtccga caatacaatt accaggaagc tagaaggagt aaggcaaaag
     2641 ttcgaagaaa ttgggcagaa aataaccgat ccggccgtaa tggccgatat gaaaaaattt
     2701 gtaagcctga ataaggagta caaagcgcta gaacccttag ttgctgccta cgaaaagtac
     2761 aagaatatac tgagcaacat cgaatcgtcc cggcaaatac ttagctccga aaaggatgag
     2821 gagctgcgag aaatggccaa ggaggaactc gatgccctta cccaggagca ggaaaagctg
     2881 gaggaagaga taaaattatt actgctcccg gctgaccctg aagactccaa gaatgccgtg
     2941 atggagatac gcgccggaac aggtggcgac gaggccagta tttttgccgg cgaccttttc
     3001 cgcatgtaca ccaagttttt tgaacggaag ggttggaagt acgaggttac cagctcttcg
     3061 gagggtacgg ttggtggtta caaggagatt gtgatgagtg ttaccggaaa cggcgtatat
     3121 ggcattttga agtacgaatc gggtgtacat cgggtgcaaa gagtcccaca aaccg
//
LOCUS       DAOOQX010000168         3152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_36879_length_3152_cov_3.579270, whole genome shotgun sequence.
ACCESSION   DAOOQX010000168 DAOOQX010000000
VERSION     DAOOQX010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36879_length_3152_cov_3.579270"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /gene="era"
                     /locus_tag="PLX53_03685"
     CDS             complement(<1..387)
                     /gene="era"
                     /locus_tag="PLX53_03685"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOY72213.1"
                     /translation="MTHKSGFVNIIGNPNVGKSTLMNALVGEKLSIITSKAQTTRHRL
                     MGIVNGEDFQIVYSDTPGILKPAYKLQQAMMKFVETALEDADIILYVTDTVETPNKNQ
                     EFLNKLQQLGKPIVLAINKVDLTTQEK"
     gene            complement(472..1932)
                     /locus_tag="PLX53_03690"
     CDS             complement(472..1932)
                     /locus_tag="PLX53_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOY72214.1"
                     /translation="MEKSSFNRDVKREKRTNSQEEPTRVYRPRTLTSRKATPETENEN
                     STPSSEGEPKKFRDDYDNSGQHSEQQNEEPTQQRRKLKPIAAPAGDFPSRPHRPRYDD
                     HVGGEQPNRYTPRSGKYSETDPSTRFSRKPYEDSRPDVHKPFRSSDPYHDDKPKHFEG
                     NRHEFSDGHFSHPKNFRTNSRNFSSDRRERQGSSYNRDFDSNPAQSYRSRTFQGSYGD
                     ERKHIERKNLNTFRERSPQKDNSGEISTNPTGPIRLNRYIAISGLCSRREADVYIQNG
                     QVTVNGKVVTELGTKVNLTDEICFKGKVLKSERMVYILLNKPKDYITTMDDPHAKRTV
                     MELIEGACPQRVYPVGRLDRNSTGVLLLTNDGELTAQLTHPSFKKKKVYQVGLDRKFN
                     PNDFQKLTEGIEIDGEVVKVDAVDYVEGGDGTELGVEIHSGQNRVVRRMFDALGYGVV
                     KLDRVYFAGLTKKNLPRGKWRFLTPKEVNMLKMGRF"
     gene            2667..>3152
                     /locus_tag="PLX53_03695"
     CDS             2667..>3152
                     /locus_tag="PLX53_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72215.1"
                     /translation="MMTIYIKCVFKNSYYSLRRIKVKTSSGGTYYVGHEEVITINSGN
                     DEWLDFRLDYHKHRFTIDRNTNDQKLYLVVMLEFRDKFPFSHLDVMFRNCMRVKAVDK
                     YQFDNFENTIAITKFTSKEYSFIDYFTCTLCLLIFLGYTVMSVIYEGIDNRNFIFIIG
                     IA"
BASE COUNT          777 a          615 c          735 g         1025 t
ORIGIN      
        1 cttttcctgg gtagtaaggt caaccttgtt gattgcaagg acaatcggtt tgccaagttg
       61 ctgcagcttg ttcaaaaact cctgattctt attcggagtt tcaacggtat cggttacgta
      121 gagaatgata tcggcatcct caagggctgt ttccacgaat ttcatcatgg cctgctgaag
      181 cttgtaggca ggttttagta ttcctggggt atcggagtaa acaatctgaa agtcttcgcc
      241 gttaacaatt cccatcaacc tatgcctggt tgtttgggct ttactagtaa taatggatag
      301 cttctcaccc acaagcgcat tcatcagggt tgattttccg acatttggat tgccaataat
      361 atttacaaag cccgacttgt gtgtcatgag caattcctga ttttatgagt gttttggctc
      421 acgttaacgt gtaaggtagt aaaggctact tttctgaaca ggaggggggg gttagaacct
      481 ccccattttc agcatgttaa cttcctttgg cgtaaggaaa cgccatttac cgcgtggtag
      541 attctttttg gtaaggccgg caaagtaaac cctgtccagc ttaactaccc cgtagcccaa
      601 agcgtcgaac atgcgccgaa caacccggtt ctgtccgctg tgaatttcaa ctcctagctc
      661 ggtgccgtcg cctccctcaa cgtagtcaac ggcgtcaacc tttaccactt caccgtcaat
      721 ttcaatgcct tcggttagtt tttgaaaatc gttggggtta aacttacggt cgaggccaac
      781 ctggtacacc tttttctttt taaagcttgg gtgggtgagc tgtgcggtaa gttcgccatc
      841 gttggttagc aggagtacgc cggtgctgtt cctgtcgaga cgtccaaccg ggtaaaccct
      901 ttgaggacag gctccttcaa taagctccat taccgtacgc ttggcgtgtg ggtcatccat
      961 tgtggtgatg taatctttgg gtttgttgag caggatgtac accatgcgct ccgatttcag
     1021 aaccttaccc ttaaagcaaa tctcatcggt taggttcacc ttggtgccca gctcggttac
     1081 caccttgccg ttaacggtaa cctgtccgtt ttgaatgtaa acatcggctt cccgtcggct
     1141 gcataatccc gaaatggcaa tatagcggtt aagcctgatg gggccagtag gattggtgga
     1201 aatttcgccg gaattatctt tttgggggct tctctcccta aaggtattaa gatttttacg
     1261 ttctatgtgc ttgcgctcat ctccgtagct gccttgaaat gttcttgaac ggtagctttg
     1321 agcaggattg ctatcgaaat ccctgttgta gctggagccc tgcctttccc tcctatcgga
     1381 tgaaaagtta cggctgttgg ttctgaagtt tttggggtgg gagaagtgtc cgtcagaaaa
     1441 ttcgtgcctg ttaccctcga aatgtttagg tttatcatcg tggtaggggt ctgaactcct
     1501 gaatggctta tgaacatcag gacggctatc ctcgtatggt tttctgctaa agcgagttga
     1561 gggatcggtt tcagagtatt taccagatcg cggggtgtag cgattaggtt gctccccgcc
     1621 aacatggtcg tcgtatctcg gccgatgtgg ccgtgatgga aaatccccag caggtgctgc
     1681 gattggtttt agctttctgc gttgttgggt tggttcttcg ttctgctgct cggagtgctg
     1741 cccggagttg tcgtaatcat ccctgaactt tttgggttcg ccctcggatg atggggtgct
     1801 gttttcattt tccgtttccg gagtggcttt tcttgatgtc aaagtccttg gccggtaaac
     1861 cctggtgggt tcttcctggg agttagtccg cttctcgcgt ttaacatccc tgttaaaact
     1921 tgatttttcc attttactat ttactgttac acctgaaagg ttaactcttt aaccttgcgt
     1981 tgtttgcatc caactagttt tcaagttgat gaatgcctgt ttctcaaaaa tttgaggttg
     2041 caaatgtata aaaataatta ttacaaagga aatttattcc aaaaaaataa atgaatggta
     2101 gtacagctgg aaaccggttt aggaaaagtt gagtagccat gctatttgtt gttaggtttg
     2161 ctgtacctat gcaagcattg aacttataag caagttttct aaatatgccg gcttaattac
     2221 gcgaaaaatg cttgttacag gctgccttga tttgggtcca aatgcaacta atcatgacgt
     2281 taaccaaatg cttaccaaca tttatagtgg tagagtttga aggcagacac ttaaatgtac
     2341 atttatccat aacttggaga ggtttaacgc ttaatgttgg cgtatataga cggtaagcgc
     2401 ttgaatagca acattgaagg aatttgtact gtggataaag ttgccgttta cttgaagatg
     2461 cctgttggcc aatgttcaag ttattgatat ggaaaatttg ctatattgca ggtactttta
     2521 tttatcccga aaaattgtct ggtacttgga caaatggatg caggtttttt ttgaaatggt
     2581 tgtgcgatat tttcatcagc agtgtaaata ttttggtgaa gtatcttcaa agtgaatata
     2641 tataatgatt tgttaacagg tagcggatga tgaccattta cattaagtgc gtttttaaaa
     2701 atagctacta ttcgttaaga cgaattaagg tcaagaccag ctctggtggc acatactatg
     2761 ttggtcatga ggaggttata acaattaatt ccggaaatga cgaatggttg gacttcaggc
     2821 tggattacca taagcacagg tttactattg ataggaatac gaatgatcag aaactctacc
     2881 tggttgttat gctagaattt agggataaat ttccttttag ccatctcgat gtaatgttca
     2941 ggaactgcat gagggttaaa gcagttgaca aatatcagtt cgacaacttt gaaaatacga
     3001 ttgccattac aaaatttacg agcaaggagt attcttttat cgactatttt acctgcacct
     3061 tatgtttatt gatttttttg ggatatactg ttatgtctgt tatttatgaa ggcattgata
     3121 acagaaactt tatatttata attggaattg ca
//
LOCUS       DAOOQX010000169         3148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_36959_length_3148_cov_2.873262, whole genome shotgun sequence.
ACCESSION   DAOOQX010000169 DAOOQX010000000
VERSION     DAOOQX010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36959_length_3148_cov_2.873262"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..721)
                     /gene="ubiE"
                     /locus_tag="PLX53_03700"
     CDS             complement(11..721)
                     /gene="ubiE"
                     /locus_tag="PLX53_03700"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOY72216.1"
                     /translation="MPTPDKSSQNVEAMFNSIASNYDFLNHFLSLGIDRKWRKRLIRL
                     VAKGNPQKVLDVATGTADLAMLLAREYGGVSITGVDISENMLAIGKRKVESENLSGQI
                     SLHRASALNLPFENDSFDAAMVAFGVRNFEDLSMGLSEVYRILINGGQFAILEFTIPD
                     KFPVAQLYRFYFRHVLPLFGGIISGSRTAYKYLPESVLAFPEKENFLEIMRQVGFTGC
                     TCKSLTFGIASIYTGLKP"
     gene            complement(737..2962)
                     /gene="purL"
                     /locus_tag="PLX53_03705"
     CDS             complement(737..2962)
                     /gene="purL"
                     /locus_tag="PLX53_03705"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOY72217.1"
                     /translation="MQQHEVTMDTVRELGLLPEEFEMIKKFLGRVPNYTELSIYSVMW
                     SEHASYKNSIKWIKTLPRKGSALLAEAGAENAGLVDIGGGYACAFKIESHNHPCAVEP
                     YQGAATGVGGINRDIFTMGARPVAQLNSLRFGNPKLERTRWLMRGVVKGIGDYGNAFG
                     IPVLAGEVFFDDSFNTNPLVNAMSVGIVSKDSVVSAVAKGVGNPVYIVGSATGKDGIH
                     GATFASANITENSADDIPSIQVGDPFQEKILLEATQEVIKTGALVGMQDMGAAGIICS
                     TSEMSEKGKHGMRIDLDKVPLRQTNMEAWEILLSESQERMLMVVKKGRERDVEEILNK
                     WDIGYAVIGNVTSGDSLEFYYHNELAAKVPASSLVLGGGAPVYDREYSEPEYYKEFKK
                     FNMNQIPEPADLVEVARFLTAHPNIASKRWVYQQYDTMIGTGNMTTNFPSDAGVFNLK
                     ELNKALAMTVDCNSRYVKADPEVGTMIAVAEAARNIVCSGATPLAITDCMNFGNPYNP
                     EVYWQFVKAIKGMAKACKKFDTPVTGGNVSFYNQSSINGREEAVYPTPTIGMIGMLEN
                     KNHHTTLAFEEKGHMIFLIGKSRNDIASSEYLYSYHGIKQSPAPFFDLDEEFAIQQAL
                     LDLIARNLVQSAHDVSDGGLFIALLESAMPRNLGFDITTDAEIRTDAFLFGESQGRIV
                     VSVSPSRETHFIDYMVEHGIPFTALGHVTKSEIRIDDVSFGFVADLKQLYDTALEKIM
                     A"
BASE COUNT          828 a          820 c          702 g          798 t
ORIGIN      
        1 caaaatgaat ttaaggttta aggcctgtat atattgaagc aatcccgaaa gttaaactct
       61 tgcatgtgca acctgtaaac ccaacttgcc tcatgatttc taagaaattc tccttctcag
      121 gaaatgcaag taccgattcg ggcaggtatt tatatgctgt ccggctgccc gaaataatcc
      181 ccccgaacaa aggcagcacg tgcctaaagt agaacctgta aagctgggca acagggaact
      241 tatcgggaat ggtaaactca agtatggcaa actgcccgcc atttataagg attctatata
      301 cctccgataa ccccattgac aaatcctcaa aatttcttac cccaaaagcc accatggcag
      361 catcaaaaga gtcgttttca aagggtaggt tcaatgcact agccctgtgg agcgaaatct
      421 gacctgaaag attttcggat tcgacctttc tcttgccaat ggccagcatg ttctccgaaa
      481 tatccacccc tgtaatggaa acacctccat actcccgcgc cagcagcatg gccaaatcgg
      541 ctgttcccgt ggccacatca agaaccttct gtgggttacc cttagctacc aaccggatta
      601 accgtttgcg ccactttcta tcaattccaa ggcttaagaa atggttcaag aaatcgtagt
      661 ttgaggcaat gctgttaaac atggcctcaa cgttctggct gcttttgtca ggtgttggca
      721 taaagcgtta ggaatactag gccatgatct tctccagagc agtgtcgtaa agctgcttca
      781 aatcggcaac aaatccaaac gaaacatcgt caattctgat ctcggatttg gttacatgcc
      841 ccagagcggt aaacgggatg ccatgctcaa ccatgtagtc aatgaaatgg gtttcacgcg
      901 atggagaaac ggacactaca attcgaccct gcgactcacc aaacaggaaa gcatcggttc
      961 gaatctcggc atcagtagtg atatcgaaac ccaggttacg gggcatggca ctctcaagaa
     1021 gggcaataaa gaggccccca tcagaaacat cgtgtgcgga ttgcaccaaa ttcctagcaa
     1081 tcaggtcgag cagtgcctgc tgaatggcaa attcctcatc caggtcaaag aagggtgcag
     1141 gcgattgctt aatgccgtgg tagctgtaga ggtattccga tgaggctatg tcgttgcgtg
     1201 atttgccaat aaggaaaatc atgtgcccct tttcctcaaa agccagcgtt gtgtggtggt
     1261 ttttgttttc cagcatgcca atcataccaa ttgtcggagt agggtaaaca gcttcctccc
     1321 tcccgttgat tgacgattgg ttgtaaaagc ttacgttacc cccggttact ggagtgtcaa
     1381 actttttgca ggccttggcc attcccttaa tggccttaac aaactgccag taaacttcag
     1441 ggttgtaggg gtttccaaag ttcatgcagt cggttattgc caaaggagtt gcaccggaac
     1501 aaacgatgtt tcgtgcggct tcggcaactg caatcatggt gccaacctcg gggtcggcct
     1561 taacgtaacg cgagttgcag tcaacggtca tggccaacgc cttgttaagc tctttaaggt
     1621 tgaagactcc tgcatcggag gggaagttgg tagtcatgtt gcccgttcca atcatggtgt
     1681 cgtactgctg gtaaacccat cgtttcgagg ctatgttggg atgggctgtc agaaaccttg
     1741 ccacctcaac tagatctgca ggctcaggaa tctgattcat gttgaatttt ttaaactcct
     1801 tgtagtattc aggctcgctg tactccctgt cgtaaaccgg cgcaccaccg ccaagcacca
     1861 gcgatgaggc cggaacctta gcagccagct cgttgtggta gtagaactcg agcgaatcgc
     1921 ccgaagtaac attcccgata acagcgtatc ctatatccca cttgttgaga atttcctcca
     1981 catccctttc gcgacccttc ttaaccacca tcaacattcg ctcctgcgat tcggaaagca
     2041 gaatttccca tgcttccatg ttggtttgac gcagaggaac cttgtccaga tctatcctca
     2101 tgccatgctt gcccttttcg gacatctctg aggtggagca gataataccc gcagcgccca
     2161 tatcctgcat gcccactagt gcacctgttt taataacctc ctgggtggct tcaagcaaaa
     2221 tcttctcctg aaagggatcg ccaacctgta tggaaggaat gtcgtcggca gaattctcag
     2281 tgatgttggc cgaagcaaag gttgccccat gaataccatc cttgccggta gcagagccca
     2341 caatatatac aggattgcca acaccctttg ccacagccga tactacggaa tccttcgaca
     2401 caattccaac cgacatggca tttaccagag ggttggtgtt aaatgaatcg tcaaagaaaa
     2461 cctctccggc caaaaccgga atgccaaagg cgtttccgta gtcgccaatt cccttcacca
     2521 ctcccctcat tagccagcga gttctttcga gcttagggtt tccaaacctg agggagttaa
     2581 gctgggcaac cggacgagcg cccatggtaa agatgtcgcg gttgatgcct cctacaccag
     2641 tagcagctcc ctggtagggc tcaacagcac aaggatggtt gtgcgattct attttgaaag
     2701 cgcaagcata cccacctcct atatcaacca ggcctgcatt ctcggctcca gcctctgcca
     2761 gcaacgccga acccttccgg ggcagggttt ttatccactt tatggaattt ttatacgaag
     2821 catgctctga ccacatcacc gagtaaatgc tcagctcggt atagttgggc acccttccca
     2881 gaaacttttt tatcatctca aactcctcgg ggagtaaccc caactccctt acggtgtcca
     2941 tggtaacttc atgctgctgc atagcctgat gttcaatcat aaattattga ataaaccttg
     3001 tgccaaagat acacgacaat tttcaattta cgcaaggtgg ggagaaaaac cgaggcatgc
     3061 acccaccggc taagaattga attgataaca gtgatccaga atgtcttgaa cctgtcaaag
     3121 ctgaacaggt tttctttact ttttaccc
//
LOCUS       DAOOQX010000170         3131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_37213_length_3131_cov_3.482445, whole genome shotgun sequence.
ACCESSION   DAOOQX010000170 DAOOQX010000000
VERSION     DAOOQX010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37213_length_3131_cov_3.482445"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="PLX53_03710"
     CDS             <1..1281
                     /locus_tag="PLX53_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY72218.1"
                     /translation="SVLGMGMLNAQTGVQINFDQFGKKIEKSNATIQDPKKNAKPDAW
                     IARAELFMDVFDAQIYWAYPGMDMKTFSLLIGKPTEQTQEVVEGITVDKFVMDRAEFY
                     FANGSLDHWQVTKPVVENPLEVAYESLIKAIELDASKKKAKPIMEDLLKLKNYLINEG
                     SSCYSKKDYGCSYKCFSKVVEIGKNPLVNQKDTAIYYYAGLSAQLDGKFEECIPYYEE
                     SIKLGFTSEGSVYFNIFDAYKSLGKADEGAKYLEDGFIKYPKNTNVLFSLISYYIDKG
                     EDPSKILVYIDQAIANEPNNPSLFFAKGTLHDRLGDTDEALTSYQKAIEIDPNYFDAY
                     YNIGALFFNVGVKYVEEANKIPAKEIEKYDELMSKANVEFKKSLPYMENALKINPASK
                     EVMDALKNIYFRFRNESPEMSAKYKEIIDKLNNM"
     gene            complement(1351..1815)
                     /locus_tag="PLX53_03715"
     CDS             complement(1351..1815)
                     /locus_tag="PLX53_03715"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOY72219.1"
                     /translation="MTCVVPILFGYNAMAQVQIYNPNADAIKDLNQAIERAGVQGKHV
                     LVQVGGNWCPWCVKLNKIFTTDSTIMKVLNANYVLVKVNYSKENKNEAVLEKLEFPQR
                     FGFPVLVILDDHGKRIHTQDSGLLESGDGYDTKKIVSFLKNWTKAAIGKPAN"
     gene            2018..2350
                     /locus_tag="PLX53_03720"
     CDS             2018..2350
                     /locus_tag="PLX53_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72220.1"
                     /translation="MTKKRVVIKFGDLSPELLELFKQKYPHGYQNHVIKVEKPGGDYF
                     FAVTLDSDDASYLVKVNVKIDSKIKDEEDEKEFFGGLSDDIGSENDAFPDDMNEEDEP
                     SEEMSDDL"
     gene            complement(2374..>3131)
                     /locus_tag="PLX53_03725"
     CDS             complement(2374..>3131)
                     /locus_tag="PLX53_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72221.1"
                     /translation="YNLVVGSTVSCRVDKINCMGKVFLEPKHPYYEVNMVYNFDFVAM
                     NNSFVKGVAELVVRDIFNETYTLFLPAEVCSKISNMVPLRVLNIRKGRLQLQLAGATG
                     SCKEFEENEIIDLTIETVVEINGEKFFFLSHSSGCFALLKVKYYLHYNFEVGKQVSCQ
                     YIGIKPDGTLRVEPPNPFYRVGDTYNFTVSSVGTESNADGETASYATVFDIYGMKCGF
                     VVPAGSKVQPDQVILCKVVGYKKGRPMLMIVQK"
BASE COUNT          996 a          607 c          606 g          922 t
ORIGIN      
        1 tctgtgctgg gtatgggaat gctcaacgca caaactggcg tacaaattaa ctttgatcag
       61 tttggaaaaa aaattgagaa aagtaacgcc accattcagg atcccaaaaa aaatgcgaaa
      121 cccgatgctt ggattgctag ggcagagttg tttatggatg tatttgatgc tcagatttac
      181 tgggcatacc ctggaatgga catgaaaaca ttttcattac taattggtaa accaactgag
      241 caaactcagg aagttgttga aggcattacc gtggataagt tcgtgatgga tagagctgaa
      301 ttttactttg ctaacggctc attagatcac tggcaggtaa ctaaacctgt tgttgagaat
      361 cctttggagg tagcatacga aagcttgatc aaagccattg agcttgatgc aagcaagaaa
      421 aaggccaagc caataatgga agacttgcta aaattgaaga actacttaat caacgaaggg
      481 tcaagctgct acagcaaaaa agattatggc tgctcatata aatgctttag taaggttgtt
      541 gaaattggca aaaacccctt agttaatcag aaagacaccg caatatacta ctatgctggg
      601 ttatctgcac agctcgatgg gaaatttgaa gaatgcattc cctactatga agagtctatt
      661 aagctgggtt tcacctcgga gggtagcgtt tacttcaaca tttttgacgc atacaaatca
      721 ctaggtaagg ctgacgaagg cgctaaatac ctggaagatg gatttattaa atatcctaaa
      781 aacaccaatg ttcttttttc acttattagc tactacattg ataaggggga agacccttcg
      841 aaaattttgg tttacataga ccaagctatt gcaaacgaac ctaataaccc ttcacttttc
      901 tttgccaaag ggacactgca tgataggctt ggcgatactg acgaagcctt aacttcatac
      961 caaaaggcaa tagaaattga ccccaactac tttgatgcct actacaacat tggagcgctt
     1021 ttcttcaatg taggtgtaaa gtatgtagaa gaagccaata agattccagc taaggagatt
     1081 gaaaagtatg atgaacttat gtcaaaggct aatgttgaat tcaaaaaatc tcttccttat
     1141 atggaaaacg ctctgaaaat caaccccgcc agcaaggagg ttatggatgc actaaagaac
     1201 atttacttcc gctttagaaa tgaaagtccg gaaatgagtg caaagtataa ggagattatc
     1261 gacaagctaa acaatatgta gtataaaaga ccccgataaa gtcggggtct tttttaaata
     1321 cctatattta acataatatt aattatagga ctaatttgcc ggttttccga tggccgcttt
     1381 tgtccagttt ttaagaaagc ttactatctt ttttgtatcg taaccatctc cggattccag
     1441 caatccggaa tcttgagtgt gaattctctt gccatgatcg tcaagtatta ccagcacggg
     1501 aaatccaaaa cgctgaggaa attccagctt ttcaagaacc gcttcgttct tattttcctt
     1561 gctgtagtta accttaacca gcacgtaatt tgcgttcaac accttcataa tggttgaatc
     1621 tgtcgtgaaa atcttattca gcttcacaca ccaagggcac caatttccac ctacctgcac
     1681 cagaacatgc ttaccctgaa cgccagccct ttcaatagcc tgatttaaat ccttgatggc
     1741 atcagcattg ggattgtaaa tttgaacctg agccatagcg ttgtacccaa acagtatagg
     1801 aacaacgcaa gtcacaacta gcaataattt gtttatcatc acgatatgtt aaatagttaa
     1861 tgttgagaaa aaatgcatta aagcattgct ataaaattct acccaaaggt agtaatcgtt
     1921 tactgattgt gcttctatta aaaaatgaaa ttatttttac agggctcgtt tgcattattt
     1981 aattttggtg tataattgca aaaatttttt tcagggtatg actaagaaaa gagttgttat
     2041 caaatttggt gatctttcac cggagctatt ggaacttttt aagcaaaaat atccacatgg
     2101 atatcagaat catgtaatta aggtggaaaa gccgggaggt gattattttt ttgctgttac
     2161 cctcgattcc gatgatgcta gctacctggt aaaagtaaat gttaaaatcg actctaaaat
     2221 aaaggatgag gaagacgaaa aggaattttt tggaggctta agcgatgaca ttggtagcga
     2281 aaacgatgcc tttccggacg atatgaatga agaggatgag ccatccgagg aaatgagcga
     2341 tgatttatag gttgacttaa ctggtatatg ctctcatttt tggacgatca taagcatggg
     2401 tcgtcctttt ttgtacccta caactttaca tagtatgact tgatcgggtt gcaccttact
     2461 gcctgcagga acaacaaaac cacacttcat cccatatata tcgaaaactg ttgcatagct
     2521 agctgtttca ccatcagcgt tgctttctgt acctacactg ctaaccgtaa agttataggt
     2581 atcacctacc ctgtagaaag ggtttggagg ttcaactctt aaggttccgt ctggttttat
     2641 cccaatatac tggcagctaa cttgctttcc aacttcaaaa ttgtaatgaa ggtagtactt
     2701 tacttttaat aaagcaaaac acccgctgct atggcttaga aaaaagaatt tttctccatt
     2761 aatttcaaca accgtctcaa tagtaaggtc aatgatttcg ttttcctcaa attccttgca
     2821 gctacctgtt gctcctgcaa gctgcagctg taacctcccc ttccttatgt tgagtaccct
     2881 aagaggaacc atgtttgata tttttgagca gacctcagca ggaaggaata acgtgtaggt
     2941 ctcgttaaaa atgtccctta caaccagctc ggcaacaccc ttaacaaaag aattattcat
     3001 ggctacaaaa tcgaagttgt aaaccatgtt aacttcatag taaggatgtt ttggttcaag
     3061 gaatactttc cccatgcagt taatcttatc aacccggcag ctgactgtgc ttccaactac
     3121 aaggttataa t
//
LOCUS       DAOOQX010000171         3129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_37246_length_3129_cov_2.883865, whole genome shotgun sequence.
ACCESSION   DAOOQX010000171 DAOOQX010000000
VERSION     DAOOQX010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37246_length_3129_cov_2.883865"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLX53_03730"
     CDS             <1..490
                     /locus_tag="PLX53_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOY72222.1"
                     /translation="MILVSAPLIWLAKIVTRLVPNSNQTHHISAEELKTIASLSRKSG
                     EIEEFMNSINSTAYVVEYDLGGNVINVNDSYIRLVGVPREQIIGSHHTQNLILNDKQK
                     KEYGTFWENLKAGVSKKLRTTITSGGRSIELLETYIPVRDVDGKVFKVMKLAFEVNEF
                     QQ"
     gene            1060..2604
                     /locus_tag="PLX53_03735"
     CDS             1060..2604
                     /locus_tag="PLX53_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOY72223.1"
                     /translation="MSSNFFKTKPIQKILGEAGDGEHGFKKTLGPMNLTTLGIGAIIG
                     AGIFVLTGQAAAQYAGPAIVISFIISGLACAFAGLCYAEFSSMIPISGSAYTYAYATL
                     GEFLAWVIGWDLILEYLFAASTVSVGWSGYIVSLLYQIGVNIPPEFTAARGMELLQVP
                     ANIPGIDAGWLPKTGELVEFLANQGVNASSLPMVTSIINIPAMFIIAVISMLLIVGIK
                     QSANFNNIMVIIKVSVIVLFIALGFAFIKSSNLHPFVPERVGEWGHFGWGGILRGAAV
                     IFFAYIGFDAVSTAAQEAKNPQRDMPIGILGSLVISTVLYILVAIVLTGMVNYKFLNV
                     PAPIALAVDAMGEQYSWLRIIIKIAALAGLTSVILVMLLGQPRIFFTMAKDGLLPPVF
                     AKVHPKYRTPYLSTIITGAVAMILAGVLPISILGELVSIGTLLAFVIVCIGIIVLRKK
                     RPDLPRPFKTPWVPLVPILGAGICLLQMAALPFATWLRLIIWFAIGLLIYLFYGRNKS
                     KLNSGL"
     gene            2910..3038
                     /locus_tag="PLX53_03740"
     CDS             2910..3038
                     /locus_tag="PLX53_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72224.1"
                     /translation="MLGFCYAIRQNAKFFLQANPLGCSSARSTWTSDEGFTNIKAF"
BASE COUNT          829 a          716 c          720 g          864 t
ORIGIN      
        1 catgattctg gtttcagcgc cccttatctg gttggccaaa atcgtcaccc gtctggtgcc
       61 caacagcaac caaacccatc atatctctgc ggaggaactg aaaaccattg ccagcctcag
      121 ccgtaaatcg ggcgaaattg aagagtttat gaactccatc aatagcacgg cttatgttgt
      181 agagtacgat ctgggcggaa atgttatcaa cgtaaacgat tcctacatca ggcttgttgg
      241 tgtcccccgg gagcaaataa tcggttcaca tcatacacaa aacctgatac tgaacgataa
      301 gcagaaaaag gagtatggga ctttctggga aaacctgaag gcgggcgtaa gtaagaagtt
      361 gcgaacaacc ataacgtctg gtggccgaag tattgaacta ctcgaaacct acattcctgt
      421 aagagatgtt gatggtaaag ttttcaaggt gatgaagctg gcctttgaag ttaacgagtt
      481 tcagcagtaa aaaaatgaac cctgcttcac aggcttattt acatgctgcc gagtttgcaa
      541 gcaggttagc gcattaagaa tgccccagga taaaaccgcc gttggttatg ctactatttc
      601 cagcggcggt atttttattt ttaggttgcc atgttgtatg ccaggctagc tccatcattt
      661 ataggtaacc ctttaaacaa gcgataggtg catgctgaat aacaagtttc tgtaacacta
      721 cgattagtaa accagacgca acagctagca gggggatata caaaactttg atcattttca
      781 atagctggaa agcgtgttta aaatttgaaa aaacacttgt tgatttttcc acgggtgtgg
      841 gcttgtaacc taattaaact agacttttct aaatatcttc aggtttagct ttacatattc
      901 cgcatttttt tttcaacgta tgattttttt tacaacattt gcctaagcta aaacaatcta
      961 ccatacaaca gcaataaata gccatagcag gatggcctgc caatcgtgcc aatagcacga
     1021 aagaagggca ttataggttt tatttaaaac attaagagga tgagctctaa cttttttaaa
     1081 accaaaccca ttcaaaagat acttggcgaa gctggagacg gcgaacatgg gtttaagaaa
     1141 acccttggcc cgatgaatct caccactcta ggcataggtg ccataattgg tgcaggaatt
     1201 tttgtgctaa cgggtcaggc ggctgcccag tatgcaggcc cggccatcgt aatctcgttc
     1261 atcatatcgg gtttggcctg tgcatttgcg ggtttatgct acgccgagtt ttcctccatg
     1321 attccaatct cgggaagcgc ttacacttat gcctatgcaa ctctgggtga gtttttggca
     1381 tgggtaattg gatgggacct aatcctcgag tacctttttg ccgcttcaac cgtgtcggtg
     1441 gggtggtcgg ggtatattgt cagcctccta taccaaattg gggtgaacat accccctgag
     1501 ttcacggcag ccagggggat ggagctgctg caagttcctg caaatatacc gggtattgat
     1561 gcgggatggc ttccaaagac cggggagctt gttgagttcc tggcaaacca gggggttaat
     1621 gccagctcgc tgcccatggt aacttctatt atcaatattc cggccatgtt catcatagcc
     1681 gtaataagca tgctgctgat agtgggcatc aagcaatcgg ccaacttcaa caacatcatg
     1741 gttatcatta aggtatcggt tattgttctc ttcatagcgc tgggctttgc ctttattaaa
     1801 agttcaaatc tgcatccatt tgtgcccgaa agggttggtg agtgggggca ctttggctgg
     1861 ggcggcattc taaggggtgc agcggtaatc ttttttgcct acatcgggtt cgatgcggtg
     1921 tcaacggctg cccaggaggc taaaaatccc caacgcgaca tgccgatagg tattctgggt
     1981 tcgctggtta tctcaaccgt gctctacatc ttagtggcta ttgtattaac aggcatggtc
     2041 aactacaagt tcttaaatgt tcctgctcct atagctcttg cggttgatgc aatgggtgaa
     2101 cagtacagct ggctccgtat aatcattaag attgcagcgc tggccggatt aacctcggtt
     2161 attctggtca tgcttcttgg ccagccaaga atattcttca ccatggcaaa ggatggattg
     2221 cttccacctg tattcgccaa ggttcatcca aaataccgca caccctacct tagcaccatc
     2281 ataaccggcg cagtggcaat gatacttgca ggcgttttac ccatcagcat tctgggcgag
     2341 cttgtttcaa ttggtaccct tttggcgttt gttatcgtct gcatcggcat catcgtgctg
     2401 aggaaaaaac gtcccgatct tccccgcccg ttcaaaaccc cctgggttcc gcttgtgcca
     2461 attctgggtg caggtatctg ccttctgcaa atggccgcct tgccctttgc cacatggctg
     2521 aggctgatta tctggtttgc cattggtttg cttatctacc tgttttacgg caggaataaa
     2581 agcaagctga actcggggct gtagcagcaa catgtttatg aatgcacacc ggataaatat
     2641 ttacagtact cgtgcagctg ggcctacctc cttaaaatgg gaaaaagcca aatgcagctc
     2701 caacacttgt tttaatgatc aacgctgtcg ctctaccatc aaaaaaacag gcatcaaact
     2761 cctgcccatc gagtacgagt aaatgataaa ttattaccat acgatgctgc aggaaagcaa
     2821 gaaaacaagg gtgtcccgaa ccgtagaaaa aatttgattg tagctgtaac tccaggggtt
     2881 atctacttca aagacaaccc catgcctaca tgttaggctt ctgctatgcc ataaggcaaa
     2941 acgctaaatt tttccttcaa gccaacccgc tcggctgctc ttctgcccgt tcaacatgga
     3001 caagcgatga ggggttcacc aacatcaagg ctttttagcc aaaaaggggg tcaggtcgca
     3061 aaattcagac ctaaccccta ggccattggt tgtggtagaa aatgaataac gtggttattc
     3121 tgtttttat
//
LOCUS       DAOOQX010000172         3106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_37614_length_3106_cov_3.232711, whole genome shotgun sequence.
ACCESSION   DAOOQX010000172 DAOOQX010000000
VERSION     DAOOQX010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37614_length_3106_cov_3.232711"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(669..2822)
                     /locus_tag="PLX53_03745"
     CDS             complement(669..2822)
                     /locus_tag="PLX53_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOY72225.1"
                     /translation="MEIQEMAAQHVLLKSWSYQWKPLFRGYTDKTLYINVGNNEVMEK
                     PVPPEVKEKFIGGKGYDLRLLWDATKPTTKWNDPENEIVISSGPIGGITQYSGTGKSI
                     VCTISPQTDIVIDSNVGGFFGPFLKFSGFDAIEIQGKAKRDVIIYIDGITHKVELFEA
                     PAEPIDSHVLGEVLSEMFANDEADKKNIGIVSTGVAAEHSLFGMLNFTFYDPKRKCIR
                     LKQAGRGGIGTVFRDKKIKAIVCKIPGVKGNLNNVADLEAIQERGRRFNKEIRELDDK
                     QCQMRQKGTAHLVEIMNDYDLLPTHNFQYGSHKEAVKIHSEVWKSYFTQGVPDGCWIG
                     CNMACAKGVDKFKLKTGPYKGQEVIVDGPEYETAAGLGSNGGMFDPEYVIEANFYCDT
                     YGICTITWGTTLAFILECYQRGVLNKERTGGLDLTWGNTSDALELLHQLARGEGFGKI
                     AGLGVHKLKEYFIREFGADPQLMQDIGMENKGLEYSQYVSKESLAQQGGYAMTNKGPQ
                     HDEAWLIFMDMVNNQIPTFEAKAEALHYFPMFRTWFGLQGLCKLPWNDVEPANNAETD
                     EPAKVPEHVDNYVTIYNAVTGSKIDKHEIIRQSERVYNFQRIFNIRRGYGLRKNDAQP
                     YRAAGPVTREEYLSREERYDKQLKELVGVDPAKMTLEEKMAALRKYRENRYEQLLDAV
                     YKRRGWNKNGVPTIAHLKEIGMDLPELIEVVTPLQ"
BASE COUNT          808 a          741 c          645 g          912 t
ORIGIN      
        1 aaaaactgct gaaacgatgg agatgagttt tgatcttaag tggatagatt atcacctgat
       61 tatataagct attcatttct tgtattttcc ctcaggtaaa aaaaccacat ccgattaact
      121 aaaaaacaga aagttaagcc aatgcagacg gctatcgcta aatttttaaa tcaaaatcat
      181 tacgtcaagt acaaattaag ctgtgtttgc ctgtcaaatt tagaggaaat ttaaacaaaa
      241 tttaacctga agtataattg atgagctgcc ttggcaaggc attttaaaca gcaatagctg
      301 tgtaaacatt acacgctcat tctctatcat gatgaaatcc tgatatttgg acacttcccg
      361 gagttgatgc aaggtaatgc cagcagcgca gcccatccct cggggtgtag cccttgccag
      421 aaggtgaaaa tcctcatcat agtcttcagt ttttagctga ttaatggctt attaaactca
      481 taagcagaga tatgatagat tagctggagt atggaacaac attttttcaa aaattgatat
      541 tctatctggt tatattttaa agtgttaaca gtagtatggc aaacgtacgg gtaataaaaa
      601 tggggttaac ccgtaggcta accccaaaac caactcacgc taacaacttg taatgtttac
      661 actgttgact actgcaaggg agtaaccacc tcaatcaatt cgggcagatc catgcctatt
      721 tccttaagat gggctatagt gggaacaccg tttttattcc atcccctacg tttgtaaacg
      781 gcatccagta gttgttcgta gcgattttcg cggtactttc taagtgcggc cattttttct
      841 tcaagagtca tttttgcggg gtcaacgcca accagctctt tgagttgctt gtcgtatcgc
      901 tcctcacgac tcaggtactc ttcccgggta accggaccgg cagccctgta gggttgggcg
      961 tcgtttttac gaaggccgta gccccgacgg atgttgaata tacgttgaaa gttgtacacc
     1021 cgttcgctct gccttattat ctcatgctta tcaattttac tcccggttac ggcattgtag
     1081 atggtaacat agttgtcaac atgctctgga accttggcag gttcatccgt ttcggcatta
     1141 ttggcaggtt caacgtcatt ccacgggagc ttgcatagcc cctgcaatcc aaaccaggtt
     1201 ctgaacatcg ggaagtagtg gagggcctcg gcctttgcct caaatgtggg gatttggttg
     1261 ttcaccatat ccataaaaat cagccaggct tcatcgtgct ggggtccctt atttgtcatt
     1321 gcgtagccgc cctgctgggc tagcgactcc ttcgaaacat actggctata ctccaacccc
     1381 ttgttctcca taccaatatc ctgcatcagc tgaggatcgg cgccaaactc cctgataaag
     1441 tactccttta gcttatgaac tcccaagcca gcaattttgc caaaaccctc accgcgggct
     1501 agctggtgaa gaagttccag cgcatcgctg gtgtttcccc atgttaaatc gagtcctcct
     1561 gtacgctcct tattaagaac ccctcgctgg tagcattcca gaataaaggc cagcgtggtg
     1621 ccccaggtaa tagtgcaaat gccataggtg tcgcagtaaa agtttgcctc aatcacgtac
     1681 tccggatcaa acattccgcc gtttgatccc aagccagctg ctgtttcgta ttcaggcccg
     1741 tcaacaatta cctcctgtcc tttatagggt ccggttttta gcttgaactt atccacgccc
     1801 ttggcacatg ccatgttgca gccaatccag cacccatcgg gaacaccctg ggtaaagtag
     1861 ctcttccaaa cctccgagtg aattttaacg gcttccttat gagaaccgta ctggaagtta
     1921 tgggtgggta gcaggtcgta gtcgttcatg atttctacca ggtgggctgt tcccttttgg
     1981 cgcatctggc actgcttatc gtccagctcg cgtatttcct tgttgaaccg cctaccgcgt
     2041 tcctgaattg cctccaaatc ggccacatta ttaagatttc cctttacccc gggaatctta
     2101 caaactatag cctttatttt cttatcgcga aataccgtgc caattcctcc ccttccggct
     2161 tgcttaagcc tgatacattt ccgctttggg tcgtaaaagg taaagttcag cattccgaaa
     2221 aggctatgtt ctgcagcaac tccggttgac acaatgccta tgttcttctt gtcggcctca
     2281 tcgttggcaa acatttcgct tagcacctct cccaacacat ggctgtctat gggttcggca
     2341 ggcgcctcaa aaagttcaac cttatgggtt atcccatcaa tgtaaattat cacatcacgc
     2401 tttgctttac cctgaatctc aatagcatca aatcccgaaa atttaaggaa tgggccaaag
     2461 aaacctccca cgttggagtc aatcacaata tcggtttggg gtgaaatggt acatacaatg
     2521 gattttcccg ttccggagta ctgggtaatt ccaccaattg gacccgagga tataacaatt
     2581 tcgttttcgg gatcattcca ctttgtggtt ggcttagtgg catcccaaag caagcgcagg
     2641 tcgtagcctt taccaccaat aaacttttcc ttcacctccg ggggaacagg tttttccatc
     2701 acctcattgt tgcccacgtt gatgtaaagt gttttatcgg tgtaacccct gaaaaggggt
     2761 ttccactggt aactccagct cttcagaagc acatgctgtg cagccatttc ctgaatttcc
     2821 atactcatcg tttttagtct tgcttgctac tactaaaatt cttcaggtaa aaatacaggc
     2881 aaatttaccg ataaaaaatg attattgtca atatgaaaat atttgcatat tgagcaacga
     2941 tgggactttc aaaagtttta caaggtagaa aaccttctgg ttgcaacagc tcaaaatgga
     3001 ctattcctgt tcaaaagaaa ttatggttgt ttgcgaatac tctccattgg gcataagtag
     3061 tgttgttggg aaatcgggca tattaggggt gtttggaaaa tgctgg
//
LOCUS       DAOOQX010000173         3105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_37635_length_3105_cov_2.985902, whole genome shotgun sequence.
ACCESSION   DAOOQX010000173 DAOOQX010000000
VERSION     DAOOQX010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37635_length_3105_cov_2.985902"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..681)
                     /locus_tag="PLX53_03750"
     CDS             complement(97..681)
                     /locus_tag="PLX53_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOY72226.1"
                     /translation="MDYNTQRKKLALPEYGRHIQQMVDYIASVEDRDERNRLAKSLVA
                     IMGNLNPHLRDINDFKHKLWDHLFIMSDFKLDIDSPYPIPSLEQYNEKPRRVPYPGNP
                     IKFKHYGRIIEMMIEKAVAMEEGPEKDALKQLIANQMKKANITWNKESVSDDDIFRDM
                     QTLSNGMLVMAPGSKLVDNKEFKNKPRTKYHKKH"
     gene            complement(894..>3105)
                     /locus_tag="PLX53_03755"
     CDS             complement(894..>3105)
                     /locus_tag="PLX53_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOY72227.1"
                     /translation="GSGKTRVLTYRIAHLLNLGVPPWSILALTFTNKAAREMKERIAK
                     IVGDSDAHKLWMGTFHSIFARILRSEAELLGYSKNFTIYDTTDSRNLIVQIIKELGLD
                     SQVYKPGEVLSRISSAKNNLVTPQAYAANSSIIAQDVKARKSEMSRLYSLYTQRCFKS
                     SAMDFDDLLLNINILFRDFPDVLLRYQNRFRYILVDEYQDTNFAQYFIVKKLSEKYRN
                     VCVVGDDAQSIYSFRGAKIENILNFRNDYPDYKLFKLEQNYRSTQTIVNAANSVIENN
                     QKQIKKVAFSANEVGDKIKLIKAYTDQEEGFLVASEILNTIRDKSCAYSDIAILYRTN
                     AQSRIFEETLRKRNIPYRVYGSLSFYQRKEIKDLLAYFRLSVNPNDDEAFVRIVNYPA
                     RGIGSTTLQHLQELANVQNISLWETAQQLKQLNHQLKPAAVKKLTDFVGMVGGFQSAV
                     YTADAYEAAYSIARNSGILTDLKADKTQEGLSRLQNIEELLNSIREFIEARRNEGVDE
                     TTTLVDYLENVALLTSLDNDDPNDRNRISVMTIHSAKGLEFDYVFLTGLEENLFPSNM
                     SMNTPEELEEERRLFYVALTRAAKGVTISFAQTRLKWGNINISNPSRFISEIDQRYVE
                     SPFEPDGGGHLKATSELSRQTSINNFGKNSIGNRVAPYSQPPGRTPVSVPENLKELPP
                     EEICEGVEVYHGKFGQGKVLSVEGEGANKKATVLFTEAGQKNLLIKFANLKGIA"
BASE COUNT          747 a          711 c          761 g          886 t
ORIGIN      
        1 gcaaataacc tgtagcgcct cattcttagc tccctgagga aataagtcgc cttttagacg
       61 cttaccgcca attacctcga acgttgccat gccaccctaa tgttttttat ggtacttggt
      121 gcggggtttg tttttaaatt ccttgttatc caccagcttt gatccgggag ccattaccaa
      181 catgccgttg cttagggtct gcatatcgcg aaaaatatca tcatcgctca ccgactcctt
      241 gttccatgta atgttggcct ttttcatctg gttggcaata agctgcttga gggcatcctt
      301 ctcgggtcct tcctccattg ctacagcttt ttcaatcatc atttcaatta ttcgcccgta
      361 atgcttaaac ttgatagggt tacccgggta tggaacccta cggggctttt cgttgtactg
      421 ctccaggctg ggaatggggt atggagagtc tatatcaagc ttgaaatcgg acatgatgaa
      481 caggtgatcc cataacttat gtttaaagtc gttaatatcc ctgagatggg ggttaaggtt
      541 ccccattatg gcgacaaggg attttgccag cctgttgcgc tcgtccctat cttcaacgga
      601 tgcaatgtag tcaaccattt gctgtatgtg ccttccgtac tccggaagag cgagtttctt
      661 tcgctgggtg ttgtagtcca ttagaaatat ttaaaaggta aaaataaaaa atttcgggat
      721 gtatgtaata aatagctggc ggtaagccta atgcagtaca tcattttatg ctgcaaagtt
      781 aggttatcgg ttgtaaaaat gcaaactgcc tgaaagggtt aagcatcctc acgtgactta
      841 agggattgaa aaagttggca cctgcagcat cagcctttcc ggttaagctg aagctaagct
      901 attcccttca ggttggcaaa cttaatgaga aggttttttt ggcctgcctc cgtgaatagc
      961 acggttgcct ttttgttagc gccttcgcct tcgacgctta aaaccttacc ctggccaaac
     1021 tttccatggt aaacctccac gccttcgcaa atttcctcgg gtggaagctc ttttaggttt
     1081 tccggaacgg atacgggtgt cctgcccggg ggctgtgaat agggagcaac ccgatttcca
     1141 attgagtttt tcccaaaatt gtttatgctg gtctgtcggg atagctcact ggttgctttt
     1201 agatgacctc caccatcagg ttcaaagggg gattcaacgt agcgctgatc aatctcgctt
     1261 ataaaccggc tgggattgga aatgttaatg tttccccatt tgagccgggt ttgggcaaat
     1321 gaaatggtta cccccttggc tgcccgggta agcgcaacat agaacaaccg gcgttcttcc
     1381 tccagctcct ccggcgtgtt catggacatg tttgaaggga aaaggttctc ctccagccct
     1441 gttaggaaaa cgtaatcaaa ctcaagcccc ttagccgaat ggatggtcat aacgctaata
     1501 cggttccggt cgttggggtc atcgttgtcc aatgaggtga gcagtgcaac attttcaagg
     1561 tagtcaacaa gcgtagtggt ttcgtcaaca ccttcattcc tgcgcgcctc aataaactcc
     1621 ctgatgctgt tgagcagctc ctcaatgttt tgcagtcggg aaagcccctc ctgtgtttta
     1681 tcggccttaa gatcggtaag gatgccggaa ttgcgggcaa tggagtaggc cgcctcatag
     1741 gcatcggccg tgtaaacagc cgattggaaa ccgccaacca taccaacaaa atcggtcagc
     1801 ttttttacag cagcaggttt taactggtgg ttaagctgtt ttagctgttg agctgtttcc
     1861 cagaggctta tattttggac gtttgcaagt tcctgcaggt gttgaagggt ggtgctgcca
     1921 ataccccttg cagggtagtt aacaattcgt acaaaggcct catcgtcatt agggtttacg
     1981 gatagcctga agtaggcaag caaatccttt atctccttcc gctggtagaa tgataggcta
     2041 ccgtaaaccc tgtagggtat gtttcgcttg cggagggttt cctcaaatat acgcgactgg
     2101 gcgttagttc ggtacagaat ggcaatatcg ctgtaggcac aactcttatc cctgatggtg
     2161 ttgagaattt cggaggccac cagaaagcct tcctcctgat cggtataggc cttaatcagc
     2221 ttgatcttat cgcccacctc gttggccgaa aaagcaacct ttttgatttg cttctggtta
     2281 ttttcaataa cgctgttagc agcattaaca atggtttgag tagaacggta gttttgctca
     2341 agcttaaaga gcttgtagtc ggggtaatcg ttccggaagt tgaggatatt ttcgatttta
     2401 gcgcccctga aggagtaaat actttgcgca tcatcgccaa ccacgcaaac attcctgtac
     2461 ttctccgaga gtttttttac aatgaagtac tgggcaaagt tggtatcctg gtactcgtca
     2521 accaggatgt agcggaacct gttctgatac cgcagcagca catccggaaa gtcgcggaaa
     2581 agaatgttta tgtttagcag caaatcgtca aaatccatgg cagacgattt aaaacagcgc
     2641 tgggtgtaaa gggagtacaa acggctcatt tccgacttgc gggctttcac atcctgtgca
     2701 ataatgcttg agtttgcggc gtaggcctgc ggggtaacca ggttattctt agctgaggat
     2761 atacgcgaca gcacctcgcc gggcttataa acctggctgt cgagccccag ctcttttatg
     2821 atttgaacaa tcaggttacg ggagtcggta gtatcgtaaa tggtgaaatt cttactgtag
     2881 cccaggagct ctgcttcgga gcggagaata cgagcaaaaa ttgagtgaaa agttcccatc
     2941 cagagcttat gggcgtcgga atccccaaca attttggcaa tacgttcctt catctcccgc
     3001 gcggccttat tggtaaaggt cagggcaagt atcgaccagg ggggcacgcc caggttgagc
     3061 agatgggcaa ttcggtaggt aagtactctt gttttacccg atccg
//
LOCUS       DAOOQX010000174         3087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_37933_length_3087_cov_3.199868, whole genome shotgun sequence.
ACCESSION   DAOOQX010000174 DAOOQX010000000
VERSION     DAOOQX010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37933_length_3087_cov_3.199868"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..1020
                     /gene="fsa"
                     /locus_tag="PLX53_03760"
     CDS             364..1020
                     /gene="fsa"
                     /locus_tag="PLX53_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOY72228.1"
                     /translation="MKFFIDTANLDQIREANDLGVLDGVTTNPSLMAKENIRGSENIK
                     KHYVDICHIVKGDVSAEVIATDYEGMIREGKELAALHPQIVVKVPCIREGIKAIKHFS
                     DSGIRTNCTLVFSAGQALLAAKAGATYVSPFIGRLDDTSTDGVALIRQIVEIYRHYGY
                     QTKVLAASIRHTMHIIQCLEAGADVATCPLNAILGLLNHPLTDIGLQKFLDDFKKVNG
                     "
     gene            1020..1643
                     /locus_tag="PLX53_03765"
     CDS             1020..1643
                     /locus_tag="PLX53_03765"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOY72229.1"
                     /translation="MPECLYIKMYPQNLNPRDIERAVEVLKRGGVIIYPTDTVYGIGC
                     DITNTKAVERIIKIKGMKPKEANFSFICHDLSHIADFARVDNSTFRLMKKNLPGPFTF
                     ILPGLNRVPDYFLSRRKTVGIRIPNHPIPVEIVRQLGNPILTTSLKDSDEVIEYTTDP
                     ELIFENYQNLVDLVIDGGAGNNIPSTVVDCTGDEPAIIREGLGELVF"
     gene            2085..2315
                     /locus_tag="PLX53_03770"
     CDS             2085..2315
                     /locus_tag="PLX53_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72230.1"
                     /translation="MVEETLWDGFDHIISELHRACVTNHMDLPPTPSAKGACYNINLP
                     ADLNNLPAKESLGSVLSRQKQAKIKESLSGSE"
     gene            complement(2532..>3087)
                     /locus_tag="PLX53_03775"
     CDS             complement(2532..>3087)
                     /locus_tag="PLX53_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOY72231.1"
                     /translation="TSEHFGVQDQVDIYFATFAKSMAGIGAFVASQEHVIRFLAYNMR
                     SQIFAKSLPMAMVLGIQKRLEILKSHPELRENLWNIAGELQKGLREIGFDLGRTQSPV
                     TPVYMFGGIPEAANMIFDLRENFGVFCSMVVYPVVPKGQIMLRLIPTAAHTLNDVKET
                     VEAFRVVRDRLAAGKYNKEKIAQF"
BASE COUNT          885 a          730 c          678 g          794 t
ORIGIN      
        1 aatattcttg ttaagcgatt ttatgaagtt gatatagcct cgctttacag taaaaaaatt
       61 agtgcgccca ctaattccga taaggtagcg gtaacctaag aaaatttgag tagaaactga
      121 aatatcattc ggcttgttag ccgacaattc ctgattattt aatctaagag tgtcagcaac
      181 ctgttgccca aaaactttgg gaaaagtgag gagaagtaga atcgtaagaa taaaccaacc
      241 cttcatcatt ttaacatttt cagcaaaaat atgtaaacat cccaactggt gcaaccatgt
      301 taggagttgc tgccccacta aaaaatatgt agatttgcaa agaaatctaa aaatccaggt
      361 agcatgaaat ttttcattga cacggcaaat cttgaccaaa ttcgagaagc taacgatttg
      421 ggcgttcttg atggagttac caccaaccct tcgctgatgg ccaaggagaa catcaggggt
      481 agtgagaata ttaaaaagca ctatgttgat atctgccaca tagtaaaggg tgatgttagc
      541 gccgaggtaa ttgccaccga ttacgaagga atgataagag aaggcaagga gctagctgca
      601 ctccaccctc agattgttgt gaaggttccg tgcatacggg agggcattaa ggcgataaag
      661 catttctccg atagcggtat ccgtacaaac tgcacgctgg ttttctcggc aggacaggcg
      721 ctacttgctg caaaggctgg ggcaacctat gtttctccct tcattggtag gttagacgac
      781 acttccaccg atggtgttgc gcttatccgg caaattgttg aaatctacag gcactacggt
      841 taccaaacca aggtgctggc tgcctccatt cgtcatacca tgcacattat ccagtgttta
      901 gaggcaggag ccgatgttgc aacatgccca ttgaatgcaa ttcttgggct actcaaccat
      961 ccccttactg acatcggact tcagaagttc ctcgacgact ttaaaaaggt taacggttaa
     1021 tgcccgagtg cctctacatt aaaatgtacc ctcagaacct caacccccgc gatattgagc
     1081 gtgcggttga ggtgctgaag cgcggcggag tcatcattta tcccaccgat acggtttacg
     1141 gcattgggtg cgacatcacc aacaccaagg cggtggagcg catcatcaag ataaagggaa
     1201 tgaagcctaa ggaggccaat ttttcattta tctgccacga ccttagccac atagccgatt
     1261 ttgcccgggt ggataacagc acattcaggc tgatgaaaaa gaatttgccc ggcccattta
     1321 cctttatcct ccccggtctt aaccgggttc ccgattactt cctaagccgc cgtaaaacag
     1381 tgggtatccg cattcccaac catcccatac ccgttgaaat tgtccgtcag ctgggaaacc
     1441 ccatcctcac cacatcgcta aaggacagcg acgaggtgat tgagtacacc accgatcccg
     1501 agctaatttt tgaaaactac cagaatttgg ttgacctggt tattgacgga ggcgcaggta
     1561 ataatattcc ttcaacggtt gttgactgta caggcgacga accagctata atccgtgaag
     1621 ggttgggcga acttgtcttt taaccctacc atgcaacatt ccatcacgct ggctattctt
     1681 gccggtggca aagctaccag aatgggtaga gttggtaagg cttttgtaaa agttgaaaga
     1741 aaaaccaata attcaaagaa tttacgaaag cctttcgagc ttatttaccc aagtaatcaa
     1801 aatcacaaac accacaagta cagaatatcg gactccgtca aatatttacc ccgacacgtt
     1861 gaataaaggc tgtcctctag gcggtttaca ttctgccctg ttccactcct tcacggaact
     1921 tgtatttata gctgcatcgg atacgccctg ccccgataga aattttgcct aaaaaatcat
     1981 acagggtcat ttcagcatca agccaatgac ggagatattt gcacctagta taaacgggct
     2041 tgccgaacct tgatttggaa tttactcaaa aacggagcct ttccatggtt gaagaaacct
     2101 tatgggatgg ttttgatcat atcatatcag aattgcatcg ggcttgtgta accaatcaca
     2161 tggatttgcc ccctacccct tctgctaaag gcgcttgtta caacatcaac ttgcctgccg
     2221 acctgaataa tttaccagcc aaagaaagcc tgggtagcgt gttgtctaga caaaagcaag
     2281 ctaaaatcaa agaaagcttg tcgggctctg agtgaaactg tcgggtttct gacggaacaa
     2341 gtccgacctt cgacagattc gtttctccag ggactccggg cagattttgg atataaaaaa
     2401 gaaaaccctt tgcagatttc aggtgcaaag ggttaagtat tctgatcggg caacctttca
     2461 catttcagcg acttgtctag tcctcccggg gctagcatca agtattcgct tcaacgtatg
     2521 gttgctaaaa gttaaaactg cgcaattttt tccttgttat acttaccggc agctaacctg
     2581 tcgcgcacca ccctgaatgc ctcaacagtt tcctttacat cgttaagggt gtgggctgct
     2641 gtaggtatca accttagcat aatttggcct ttgggaacca cagggtaaac taccatagag
     2701 cagaaaacgc cgaagttttc acgaaggtcg aaaatcatat tggcggcctc aggtattccg
     2761 ccgaacatgt aaaccggcgt aaccggcgat tgggtacggc caaggtcaaa acctatctcc
     2821 ctaagtcctt tttgcagctc gcctgcaata ttccagagat tctccctaag ctcgggatgg
     2881 gatttcaaaa tttcgagccg cttctgaata cccagcacca ttgccatagg aagcgatttt
     2941 gcaaaaatct gtgagcgcat gttgtaggcc aggaagcgga taacgtgctc ttgtgaggca
     3001 acaaaagctc ctattccggc catactcttg gcaaaggtag caaagtagat atcaacctga
     3061 tcctgcactc caaaatgctc ggaagta
//
LOCUS       DAOOQX010000175         3083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_38003_length_3083_cov_3.562417, whole genome shotgun sequence.
ACCESSION   DAOOQX010000175 DAOOQX010000000
VERSION     DAOOQX010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38003_length_3083_cov_3.562417"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLX53_03780"
     CDS             complement(<1..480)
                     /locus_tag="PLX53_03780"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOY72232.1"
                     /translation="MKISTISKKHSHTNRINTAGVIVLKKILPLLLLFLVGFLASSAY
                     GKNSNTIVLQLKWKHQFQFAGYYAALEKGFYEKAGLNVEIRELPSNTSAIDEVLSGRA
                     NYGVACSDLLVQYMNGKPLVLLAPIFQSSPSVLLVLASSNIYTLGDLVNKTIELSAKG
                     "
     gene            complement(740..1927)
                     /locus_tag="PLX53_03785"
     CDS             complement(740..1927)
                     /locus_tag="PLX53_03785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOY72233.1"
                     /translation="MNSPRVTLKPGKEQSLKRFHPWIFSGAIKHVEGSPTEGEVVQVY
                     DPDDHFLAVGHFHTGSIAVRVLSFQQEEINTEFWTQRIAEALNLRKSAGLFPSGETSM
                     FRLVHGEGDNLPGLIVDIYGSTAVIQCHSTGMYLARQAIARALIEIFDGNIKAVYDKS
                     SSTLPRRENLHPSDGFIEGEGDTKGTFIENGHQFIVSFEQGQKTGFFLDQRENRHLLQ
                     SYAKGLSVLNTFAYTGGFSVYALAGGASEVTDVESSEAAVKALQQNINLNFSPTTNHK
                     VLIADVFDFLRTTSEFFDIIVLDPPAFAKHKDALKNALQAYKRLNLAAIRKLKPGGLL
                     FTFSCSQVVDKTDFRNAVFSASAISGREISILHQLSQPADHPINIYHPESEYLKGLVL
                     KVK"
     gene            complement(2056..2667)
                     /locus_tag="PLX53_03790"
     CDS             complement(2056..2667)
                     /locus_tag="PLX53_03790"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOY72234.1"
                     /translation="MFATSISNEEIAKLPRLVFEGETVVVSENNHLERCLAELSNSRL
                     VGFDTESRPSFKKGRSNGISLLQLASSNLAAIIRVKSTGIPPALLTFLEDSSIIKVGA
                     AIRDDLKGLKQIHTFTPRGFIDLQQVVTNYNIENVSVRKMAAIVLGGRVSKSQQLSNW
                     EAPELTTQQVEYAATDAWVCREIYIRLLQEKPGIGERKHTAMF"
     gene            complement(2671..>3083)
                     /locus_tag="PLX53_03795"
     CDS             complement(2671..>3083)
                     /locus_tag="PLX53_03795"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOY72235.1"
                     /translation="FVSENDYVAVIQKVEGLLGQHDDFAEFNYQQPEGSFETITASIA
                     EYLADIYQDLKNFTMRYQLGKEEIMNDAIWECRENFGEYWGVRLVALIRAFHTLQFGD
                     VDLENIDEPEDSSKIDTSSWFITRRQNDSNPFQD"
BASE COUNT          827 a          683 c          694 g          879 t
ORIGIN      
        1 accctttgcg cttagctcta tggtcttgtt aaccaagtcg cccagggtgt aaatattgct
       61 gctggcaaga accagcaaaa ccgacgggct gctttggaaa attggagcga ggagaaccaa
      121 aggcttgcca ttcatgtact ggacaagcaa gtcggagcag gcaacaccgt aatttgccct
      181 gcccgacaac acctcgtcaa tagccgaggt gttggatgga agttccctta tctcgacatt
      241 caatccggcc ttctcgtaaa atcccttttc aagtgctgcg tagtacccgg caaactggaa
      301 ttgatgcttc cattttagct ggagaacaat ggtgttggag ttttttccgt aggctgatga
      361 cgctaaaaaa cctaccagga atagtaatag aagtggtagt atttttttta atacaataac
      421 acccgcggta ttaatgcgat tggtatggga atgcttttta gaaatggttg aaatcttcac
      481 gtcgggctag ctaaaagaat cattatgatt tacactttta cgcaatgtta aagttacact
      541 atttattaaa aaaaatctgc atcagctttt aaaaaattac aacttacacg gcctaacggg
      601 taagcgttga attgcttcaa attcaagtgt ggccggcaat tcggatgtta gaatgtggtg
      661 ctaaaatata aaagttggga attttctgaa aaacctattt taatgtataa tgatttgcaa
      721 atgttgtatc aggttggagc tacttcactt taagaaccag ccccttgagg tattcactct
      781 cagggtggta aatgtttata ggatgatctg ctggttgtga gagttgatgg agaatggata
      841 tctcacggcc cgaaatggca ctggccgaga atacggcatt cctgaaatcg gttttatcca
      901 ccacctgcga gcaggaaaaa gtaaatagca aacctccagg tttcaacttt cggatggcag
      961 ccagattcaa gcgcttgtag gcttgcaggg catttttgag tgcatcctta tgcttggcaa
     1021 aggccggagg gtcaagaact ataatgtcga agaattcgct tgttgttcgc aggaaatcaa
     1081 aaacatcggc tattagtact ttgtggtttg tagtggggct aaagttgagg ttgatatttt
     1141 gctgcaatgc ctttacagct gcttcggaac tttcaacatc ggttacctcg gaagccccac
     1201 ctgccaaggc gtaaaccgag aagcctcctg tataggcaaa agtgttaaga acgctcaaac
     1261 ccttagcgta actttgtagc agatgacgat tttcgcgctg gtcaagaaaa aatccggttt
     1321 tctgcccctg ctcaaacgaa acgatgaatt ggtggccatt ctcaataaac gttcctttag
     1381 tgtcgccttc cccttcaata aatccatcgg agggatgtaa gttttcgcgt cggggaaggg
     1441 tgctgctgct tttatcgtaa actgccttta tgtttccgtc gaaaatctct ataagagcgc
     1501 gggcaattgc ctggcgggca agatacatac ctgttgaatg gcactgaatt actgcggttg
     1561 aaccgtatat gtcaactatg agcccgggta agttgtcgcc ctcgccatgc accagcctaa
     1621 acatgctggt ttctccgctt ggaaaaagcc cggcagattt ccttaggttt agggcctcag
     1681 ctattcgctg agtccaaaat tcagtattga tttcttcctg ctgaaagctg agaaccctaa
     1741 cagcaattga tcctgtatgg aaatgcccaa ccgccagaaa gtggtcgtcc gggtcataaa
     1801 cctgaaccac ctcgccttcg gttggcgaac cttctacatg tttaatggca ccggaaaata
     1861 tccagggatg gaatcgtttt aacgattgct ccttgcctgg tttaagcgtt actcggggtg
     1921 aattcatggt catttaatag gtaaaataaa aacataaatg gaaaagctat gtaaaagatt
     1981 agggctaaag ctaaggttat gcctggttgt atgctgtgga gttgcgttat tcccaataaa
     2041 cactcaaaaa tgcgattaaa acattgctgt atgcttacgt tcgcctatgc ctggtttttc
     2101 ctgaagtagc ctgatgtaga tctccctgca aacccacgca tcagtagcgg catactccac
     2161 ctgctgggtg gtcagctcgg gtgcctccca gttgctcagc tgctggctct ttgaaactct
     2221 tccgcccaaa actatagccg ccatttttct aacgctcacg ttctcgatgt tataattcgt
     2281 tacaacctgc tgaagatcaa taaatccacg aggtgtgaat gtgtgaattt gcttcaaccc
     2341 ctttaaatcg tcccttatgg ctgcacctac cttgatgatt gaagaatctt cgagaaaagt
     2401 cagaagtgca ggcggaattc ccgtgctttt aactctaata attgctgcaa ggtttgaaga
     2461 ggctagctgg agcagagaga ttccattgct tctacctttt ttaaatgaag gtcttgactc
     2521 cgtgtcgaat cccactaagc gactattcga aagttcagcc aggcacctct ccaggtggtt
     2581 attttccgag accacaacag tttcgccctc aaataccagt cggggtagtt tcgctatctc
     2641 ttcgttgctg atgctggttg caaacattat ctaatcctga aacgggttac tatcgttctg
     2701 acggcgggta ataaaccagc tcgacgtgtc aatttttgag gaatcttccg gttcatcaat
     2761 attttccaaa tccacatccc cgaattgaag ggtatggaaa gcccgtatga gcgccacaag
     2821 tctcactccc cagtactcgc caaaattttc gcggcattcc cagatggcat cattcataat
     2881 ctcctcctta ccgagctggt agcgcatggt aaaatttttt aaatcctggt agatatcggc
     2941 cagatactcg gcaatgctgg cggtaatcgt ttcaaaagaa ccctcgggtt gttggtagtt
     3001 aaattcagca aaatcgtcat gctggcctag taatccttca actttttgta taacagcaac
     3061 atagtcgttt tcggaaacga att
//
LOCUS       DAOOQX010000176         3079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_38064_length_3079_cov_3.072421, whole genome shotgun sequence.
ACCESSION   DAOOQX010000176 DAOOQX010000000
VERSION     DAOOQX010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38064_length_3079_cov_3.072421"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PLX53_03800"
     CDS             complement(<1..842)
                     /locus_tag="PLX53_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOY72236.1"
                     /translation="MLRKLYIQNYAIIDELTIEFQPGLNIITGETGAGKSILLGALML
                     ILGQRVDASILKDQSKSCIVEGEFDISSYDLDDFFRVNDLDYNPTTIVRRQIGESGKS
                     RAFINEIPVNLTLLKELGDRLIDIHSQHQNLLLGTSRFQLNVVDTYAIPKNLQSAYLE
                     TYLNYRQLEAELKKLEDEAANFGRDFDYLQHQLEELRAANLKEGELEELEIEQAKLTH
                     ATEIKSALEFTINAASNDETSAIALLKEASASLKKISPYYPAADELEHRLESCRIELR
                     DIVD"
     gene            complement(861..1784)
                     /locus_tag="PLX53_03805"
     CDS             complement(861..1784)
                     /locus_tag="PLX53_03805"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOY72237.1"
                     /translation="MHKSFAKLMLTLLLIAPLVLDGQELRCNVQINSQKIQGTNRTIF
                     QTLQTAIYEFMNNTAWTNHVYGFDERIECSIMLNITEQLGSDEFKGTLQVQSRRPVFN
                     SSYNTTMLNFQDNNLHFRYREFDKIEFSENSHLSNLSSILAFYAYIILGIDYDSFSML
                     GGTDYLLKAEKVVNNAQNAPERGWKAYEGNRKNRYWMIENLLNSKYRPLREVLYRYHR
                     LGLDRMSDKAVEGRAEIAECMATLQKVYREKPDPYLFALQLFFDAKSDELVNIFSESF
                     PSEKARVVNILTEIDNANAGKYQKILEQKNS"
     gene            complement(1806..3017)
                     /gene="coaBC"
                     /locus_tag="PLX53_03810"
     CDS             complement(1806..3017)
                     /gene="coaBC"
                     /locus_tag="PLX53_03810"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOY72238.1"
                     /translation="MRLAGKHIILGVTGSIAAYKAAILTRLLIKEGAEVKVIMTPHAK
                     EFITPLTLATLSKNPILVDFFNPENGGWNSHVDLGLWADLFLIAPASANTMAKMASGV
                     ADNLLLTTYLSARCPVMLAPAMDMDMYSHPATQKNIQILKSYGNIFVEAATGELASGL
                     SGKGRMEEPENILQHVVNLFLAANRFNGYKVLITSGPTYEPIDPVRFIGNYSSGKMGT
                     AIATAIAQQGAQVTFITGPAAVEPQHPSVKIVRVKTASEMLEQAKNEFDSCQVAILAA
                     AVADFAPANTSTDKIKRGKENLDLQLEPTTDIAETLGKMKRQNQIIVGFALETHNEVE
                     NAIDKLTRKNLDIIVLNSLNDAGAGFGHNTNKITLIDRNGKQKSFELKPKSEVAQDIA
                     NAIIDLLQQAK"
BASE COUNT          716 a          671 c          764 g          928 t
ORIGIN      
        1 tcatctacaa tatccctcag ctcaatgcgg cagctttcca aacgatgttc cagctcgtcg
       61 gcagcaggat agtagggcga tattttttta aggcttgcag aagcttcttt tagcagggct
      121 atggccgatg tttcgtcgtt gctagctgca tttatggtga attccaatgc cgatttaatt
      181 tcggttgcgt gggtaagttt tgcttgttca atctccagtt cctccagctc gccctcttta
      241 aggttagcag cgcgtagctc ctccagctgg tgctgcaggt agtcaaaatc tcgaccaaaa
      301 tttgctgctt cgtcttcaag tttttttagc tcagcctcaa gctggcggta gtttaagtac
      361 gtttctaggt aggcggattg caagtttttg ggaatggcgt aggtgtcaac cacattaagc
      421 tgaaagcggg aggttcccag caggaggttt tgatgctgtg aatgaatgtc tatgaggcgg
      481 tcacctagtt ccttaagaag tgtgaggtta accggaatct cgtttatgaa agcccttgat
      541 ttacccgatt cgcctatctg tctgcggaca atggtagttg ggttgtaatc caaatcgttt
      601 actcggaaaa agtcatcaag gtcgtagctg ctgatgtcaa actccccctc aactatgcag
      661 ctttttgact ggtctttcag tatagacgca tcaacccttt ggcccagaat gagcatgagc
      721 gctcccagca ggattgattt tccggctccg gtttcacctg taatgatatt caggccaggt
      781 tgaaactcta tggtcagctc atcaattatg gcgtagtttt gtatgtagag tttccgaagc
      841 atcggcacaa ggggtttagg ctagctattc ttttgctcca gtattttctg gtacttgcca
      901 gcgttggcat tgtcaatttc ggtaagaatg ttaactaccc tggccttttc ggaggggaac
      961 gattccgaga aaatatttac cagctcgtca cttttggcgt caaagaaaag ctgcagggca
     1021 aagaggtaag ggtcgggctt ttcgcggtat accttttgaa gggtggccat acactcagct
     1081 atttctgctc taccctccac tgccttatcg ctcatccggt ctagtcctaa gcgatggtaa
     1141 cggtagagca cctctcgaag aggccggtat ttggagttta gaaggttttc aatcatccag
     1201 tacctgttct ttcggtttcc ctcgtaggct ttccatcccc gctcaggtgc attctgggcg
     1261 ttgttcacta ctttttcggc tttaaggaga taatcggtac cgccaagcat ggaaaatgaa
     1321 tcgtagtcaa ttcctagtat aatgtaagcg tagaacgcca gaatggatga aagattgctg
     1381 agatggctgt tttcggagaa ctcaatcttg tcaaattctc tatagcgaaa gtgcaggttg
     1441 ttatcctgaa agttgagcat ggtggtgttg taggacgagt tgaataccgg ccggcgcgat
     1501 tgtacctgga gggttccctt aaattcgtca gatcccagct gttcggtgat gttaagcatt
     1561 atgctgcatt caatacgctc gtcgaaaccg taaacgtggt tagtccatgc cgtgttgttc
     1621 atgaactcgt agatggctgt ttggagcgtt tgaaaaatag tgcgattagt gccctgaatt
     1681 ttttgggagt taatctgcac attgcacctt aactcctgtc catcgagtac cagcggggca
     1741 atgagcagaa gggttaacat cagcttagca aaagacttgt gcatggcgat tcccgttggt
     1801 ttgtactatt ttgcttgttg aagcaggtca attatggcgt tggctatatc ttgggctacc
     1861 tctgatttag gctttagctc aaaggatttt tgcttaccat tgcggtcaat tagggtaatc
     1921 ttgttggtgt tatggccaaa accagcgccg gcatcgttca gggagttaag tactatgatg
     1981 tccaggtttt ttcgggttag cttgtcaatg gcattttcaa cctcgttgtg ggtttcaagg
     2041 gcaaaaccga cgatgatttg attctgccgc ttcatcttac cgagtgtttc cgcaatatct
     2101 gttgtcggct caagttgtag gtcaagattt tccttccccc ttttaatttt atcggttgag
     2161 gtgttagctg gagcgaaatc tgccactgct gctgcaagaa tggcaacctg acagctgtca
     2221 aattcatttt ttgcctgctc cagcatttcg gaggcggttt taaccctgac aattttaacc
     2281 gaaggatgtt gtggttcaac agctgccgga ccagtgatga aggtaacctg tgctccttgt
     2341 tgcgcaatgg cggtggcaat ggcagttccc atttttccgc tggaataatt accaataaag
     2401 cgaacgggat cgatgggttc ataagtagga cccgaggtta ttagtacctt gtatccatta
     2461 aaccggttag ccgccaaaaa aaggttgact acgtgttgta gaatattttc gggttcctcc
     2521 attctgcctt tgccggatag cccgctggct agttcccctg tagcggcctc aacaaaaata
     2581 ttcccgtaac ttttaagaat ctgaatattt ttttgtgtag cgggatggct atacatatcc
     2641 atatccatgg cgggagccag cataacaggg catctggccg agaggtatgt ggtgagcagc
     2701 aggttatcgg caacaccact tgccattttt gccatggtgt tagccgaggc tggagctatc
     2761 agaaacaggt ctgcccaaag tcccaaatca acatgactat tccacccgcc attctcgggg
     2821 ttaaagaagt caaccagaat ggggtttttt gaaagggttg ccagggtaag cggggtgatg
     2881 aactccttgg catgaggggt cataataacc ttaacctcag caccttcttt aatcagcaga
     2941 cgtgtaagaa tagctgcctt gtaggctgca atgctgccgg taacgccaag aattatatgt
     3001 ttccccgcca gacgcattac tggttggtaa cttcggtgtt atcaaccatt ttgtagtaaa
     3061 gctttccatc cataaattc
//
LOCUS       DAOOQX010000177         3074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_38165_length_3074_cov_3.328254, whole genome shotgun sequence.
ACCESSION   DAOOQX010000177 DAOOQX010000000
VERSION     DAOOQX010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38165_length_3074_cov_3.328254"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLX53_03815"
     CDS             complement(<1..662)
                     /locus_tag="PLX53_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HOY72239.1"
                     /translation="MYDILELNKKLLPELREIAKQLNIKKLESLKKQDLIYKILDQQA
                     INASMPGKGKEKERPSTSRPAVKLQPTEVVNKEQNAEAPLQQERSDSRDSAGKRVRFT
                     RNKSAEVKKEYSSTPARGEKVSKFSKQPDANELSIRTEQSNFPSSKEVFLPEVDLEPQ
                     TQREKKEDEVFADVLADDTQGIPEAEVSQSDSTPNIKIEEENFPLNPQESAVSNQLPE
                     PAG"
     gene            1146..2009
                     /locus_tag="PLX53_03820"
     CDS             1146..2009
                     /locus_tag="PLX53_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOY72240.1"
                     /translation="MRQLKIIKQVTNRETVSLDKYLHEIGKVDLLSTDDEVALARKIK
                     EGDPEALSKLVKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQARIIKLPLNKIGSINKVNRALVELEQKFEREPTINELSE
                     VLMLAPEDIKEALRSSGRPISMDAPLTQDEDSSMYDVILSPDSPQPDRGLLNESLRRE
                     IERALSTLTPRESSIIRMYFGLNGKQPLTLEEIGEELDLTRERVRQIKEKALRRLKQT
                     TRSKILKSYLG"
     gene            2109..2759
                     /gene="rpe"
                     /locus_tag="PLX53_03825"
     CDS             2109..2759
                     /gene="rpe"
                     /locus_tag="PLX53_03825"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOY72241.1"
                     /translation="MTHLVAPSLLSADFANLSADIEMVNNSLADWFHLDIMDGRFVPN
                     ISFGFPVVERIRDKASKPLDVHLMIVDPDRYLERFHDAGASWLTVHYEACTHLNRTLD
                     QIRKLGMKAGVALNPHTPVELLIDILDYTDLVLIMSVNPGFGGQKFIDSSLGRIERLR
                     AMIDKRGLSTLIEVDGGVDTNNASALYRAGANVLVAGNAVFKANKPLDVIAQLRKA"
     gene            complement(2756..2935)
                     /locus_tag="PLX53_03830"
                     /pseudo
     CDS             complement(2756..2935)
                     /locus_tag="PLX53_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          848 a          663 c          694 g          869 t
ORIGIN      
        1 cctgcaggtt cgggtagctg gttgctaaca gccgattcct gcgggttaag aggaaaattt
       61 tcttcctcga tcttgatatt aggagtgctg tcggattggc tcacttcagc ttcaggtatt
      121 ccttgcgtgt cgtcagcaag tacatcggca aaaacctcat cttccttttt ttctctttgg
      181 gtttgaggct ccaggtcaac ttcaggaaga aaaacttctt tacttgaggg aaaattggat
      241 tgctcggttc ttatagataa ttcattcgca tcaggctgtt ttgaaaattt ggacaccttc
      301 tctcctcttg ccggagtaga agagtactct ttttttactt ctgcgctttt gtttcgggta
      361 aaccgcactc gtttccccgc gctatccctg ctgtctgatc tttcctgctg tagtggggct
      421 tcagcattct gctccttgtt cactacttcg gtaggctgga gctttacagc cggtcgggaa
      481 gtgcttgggc gctccttttc cttgcccttc cctggcattg aagcattgat agcctgctga
      541 tccaaaattt tataaatcaa gtcttgcttt ttcaggcttt caagcttttt gatatttagc
      601 tgcttggcaa tttcccgcag ttcgggaagg agcttcttgt tgagttcgag aatatcgtac
      661 ataaaaatag tttagttgta aaatgaaggt tttagagtag ttaaagaaaa attggaaaaa
      721 atactttgca taggcttgta aaatggtaat caatgggcaa gccttttgct tacatcctag
      781 tgtaatgctt aatataaata cgttaacata ttgtaaagct gtcaggtaaa taactttaga
      841 tgaaattact cagtgatgaa atagttgttg aagtgaaatt gaagaaagag ggttggagtt
      901 ctcttcccgg ttattttttg caaaggtatg aaatattatt tttcacctgc aagaatttta
      961 tgatgatttt ttgttgaata taaaataaaa accattatag aacatggcat tgactattaa
     1021 aaaaatggca ttagatggaa accgccacca aaaggtttaa aaaaaattac ataaaaaaat
     1081 cgaagcatta acaaaattta ataattgtaa ttatattcac ccccaaacaa agcatcgtta
     1141 ttggaatgag gcagctaaaa ataatcaagc aggttaccaa ccgtgaaacg gtatcgctcg
     1201 ataagtatct gcacgaaatt ggcaaggtgg atttgcttag tacagacgat gaggtggcac
     1261 ttgcccgaaa aattaaagag ggtgatcctg aagcgttatc caagctggta aaagccaacc
     1321 tcagatttgt agtatcggtt gccaagcagt accagaacca gggtttaagc ctacccgacc
     1381 tgataaacga gggtaatctg ggacttatta aagcagctca gcgctttgac gaaacgaggg
     1441 gatttaaatt tatatcctac gccgtttggt ggattaggca gtctattctg caggcgctgg
     1501 ccgagcaggc aagaattata aagctacctt tgaacaaaat tggctcaatt aataaggtga
     1561 atagggcgct ggttgagctg gagcaaaagt ttgaacggga acccaccatt aatgagctgt
     1621 ccgaggtgct aatgcttgcc cccgaggata tcaaggaggc cctgcggagc agcggccgtc
     1681 ctatttccat ggatgctcca ctgacacagg atgaagatag tagcatgtac gatgttatcc
     1741 tttcgcccga ttctccccaa cccgacaggg gattacttaa tgaaagttta agacgagaaa
     1801 tagaacgtgc cctatcaacc ctcacgcctc gtgagtccag tatcataaga atgtacttcg
     1861 gtctaaacgg aaagcaaccc ttaaccctcg aggaaattgg cgaggagctc gaccttaccc
     1921 gcgaacgggt acgccagatt aaggagaaag cgcttcgaag gctcaagcaa accactcgta
     1981 gcaaaattct caagtcatac ctgggttagc cccgcttagc ccttccgttt ttctctccaa
     2041 catgccttaa agcattcggg gcaacaccta gtattttaaa ttgctacctt tgcaaaaacc
     2101 gattgcttat gacccatctt gttgctccat cgctgctctc ggcagatttt gcaaatcttt
     2161 ctgctgatat tgaaatggtt aacaatagcc tggcagattg gtttcacctg gatatcatgg
     2221 atggcaggtt tgtgccaaat atatcatttg gatttccggt agtagagcga atcagggata
     2281 aagccagtaa acccctggat gtccacctga tgattgttga ccccgacagg tacctggagc
     2341 gattccatga tgccggtgcc agctggctta ccgtacacta cgaggcttgc actcatttaa
     2401 accggaccct tgaccagatt cgaaaattgg gcatgaaggc cggagttgct ctcaaccccc
     2461 acactcctgt tgagctgctg atagacatcc tggattacac cgatttggtg ctgataatgt
     2521 cggtaaatcc ggggttcggt ggacaaaagt tcatagatag ctccctaggc aggattgagc
     2581 gactgagagc aatgatagat aagcgggggc taagcaccct gattgaggtt gatggaggcg
     2641 ttgatactaa caatgcatcg gctctataca gagccggagc caacgtgctg gtggccggga
     2701 atgctgtgtt taaagccaat aaacccctgg atgtaattgc tcagctcagg aaggcttagt
     2761 ccgccacctc caacctttcc cgaagggcta caagcttttg aacttttaag aagagacggt
     2821 ggctttcttc aaaattcagt gtttgctgcc catcggatag cgctctttcg ggtgtttgat
     2881 gaacttcaag tagtagccca tccgcccctg ccatcactgc tgccaacgac ataggctcta
     2941 catattccct acgctactaa ccatcgcgac catattacaa cgtgcaggaa ttgtaatttt
     3001 atacaacgtt taacttatgc tgtccccttt tccgatgaag atttaggcta aacaagattt
     3061 tacaaaaaaa ttgt
//
LOCUS       DAOOQX010000178         3069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_38257_length_3069_cov_2.952555, whole genome shotgun sequence.
ACCESSION   DAOOQX010000178 DAOOQX010000000
VERSION     DAOOQX010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38257_length_3069_cov_2.952555"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PLX53_03835"
     CDS             complement(<1..324)
                     /locus_tag="PLX53_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOY72242.1"
                     /translation="MLGLSKKGNTGVSLVLSSGGARGLAHIGVIEELLTRDFCINSVV
                     GSSMGALVAGLYASGGLENFKNWAVGLSKLEMLSLVDFTLTSRGIIKGEKVFNQMQKL
                     GLIPDI"
     gene            complement(393..887)
                     /locus_tag="PLX53_03840"
     CDS             complement(393..887)
                     /locus_tag="PLX53_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOY72243.1"
                     /translation="MSTKEQTLENDEKFMDEAIELAIKGVMSDEGGPFGAIVVKDGRI
                     VGKGNNHVTSTNDPTAHAEVMAIRDACRTLNTFQLNGCVLYTSCEPCPMCLGAIYWAR
                     LDKVFYSANRHIASYHGFDDSFIYNEVNLSFDKRRIPFINISPEKGLVPFREWEKKDD
                     KIKY"
     gene            1114..>3069
                     /locus_tag="PLX53_03845"
     CDS             1114..>3069
                     /locus_tag="PLX53_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72244.1"
                     /translation="MRFEVKFPAIHLHIILFVVFAFSLNRLQGQSALHITNYDKKEYR
                     AANQNWSVSLGGISAVFTGNNHGLLCYDGAEWLLKPVPGKSIIRAVLYDSSRVYVGLL
                     DEFGYFDIVNGKPTIYHCLSTTVSNNLKNQEYWRIAKHHGKIYFQSFGSLYVYDGREV
                     SSVPLDFPILLLIKANDELFTQRIGGGLYRITENGLKLLEGSEIFADTEIKAALTHQN
                     DILFCTNSMGIYKFNGFSFEPWHTAVDAELKTYNLNTAVETQHYYCIGTILKGVLVLD
                     HNGQLVYQLDSKHLQNSTILSLEKDKMGNIWVGKDKGISYVAFNSPVSIHIDDTDKFS
                     CYSGVLVGSYLYLGTNQGIFRYSFSSENMLTGKTLVTGSQGQVWFIKLIDGKLYCGLN
                     DGTYLLENDKLEKIGFVSGGYNLEKFSKEKDLLIQSTYNNLVTYRNNNGRWEQNKILQ
                     GFSSPSRFLQVDFQQNIWVGHSIVGVNRLQTNSNVDSIISIENIGQEHGLNFQTNRIY
                     KIENRIVIPTGSGFLRWNDLNERFEPFGELNAQLQGFEKAHAVASLPDDKYWLIKDDE
                     WGLFEIRFGKANLLYRVIPDMFNMELVEENEKIIQLNDSLQLVCLDNGFAILNILQLN
                     RLPEVNLPPNINDVRFWRNEKDSASIKP"
BASE COUNT          833 a          789 c          684 g          763 t
ORIGIN      
        1 gatgtcggga attagcccca gcttttgcat ttggttgaaa accttttcac cctttatgat
       61 acccctggag gttagggtga aatcaacaag cgagagcatc tcaagtttac tgagtccaac
      121 agcccagttc ttaaaattct caagccctcc cgaggcgtac aaaccagcca ccaaggcacc
      181 cattgatgag ccaaccaccg agttgataca aaagtcccgg gtaagtaact cctcaataac
      241 cccaatgtgt gctaaccctc tggctccacc gctcgagagc accagggaaa cacctgtgtt
      301 accttttttc gacagcccta acattgcctg ccactcataa tggtgcattt atacaacccc
      361 aacctgctgc cggaacaaaa tgggctaacg ggttagtact taatcttatc atccttcttt
      421 tcccactccc tgaagggaac caaccccttt tcggggctaa tgttgataaa gggaatgcgt
      481 cgtttatcga acgaaaggtt aacctcgttg taaatgaagc tatcgtcgaa accatggtac
      541 gaggcaatat ggcggttggc cgagtagaac accttatcga gacgagccca gtaaatcgct
      601 ccaagacaca tcggacaagg ctcgcacgag gtgtaaagca cgcacccgtt cagctgaaag
      661 gtgttaagtg tccggcaggc atcccttatc gccataacct cggcatgggc ggtagggtcg
      721 ttggtggagg ttacatggtt gttgcctttg ccaacaatcc tgccatcctt aaccactatt
      781 gccccaaacg gcccaccctc atcggacatc acccccttga ttgccagctc aatggcttca
      841 tccataaatt tctcgtcgtt ctccagcgtt tgctccttag tactcatcta taatcttcat
      901 ttttgttcaa cctgtaaaaa tataacaaat tatccaaccc cacaaaggcg gcagggagtt
      961 ttggggaaaa ttcagcccct ttcaatactg tcagcagcat aaaattgaac tctacggata
     1021 agttagccct taaaaattac cctctgaatt ttcagcgtat gctttgcttt taggtaataa
     1081 gttctaactt tgttacgatc caatttcaaa cgtatgcgct tcgaggtaaa atttccggca
     1141 atccatcttc atattattct ttttgtagta tttgcattca gcttaaatcg gctacagggg
     1201 caatcggccc tacacataac caactacgat aaaaaggagt atagggctgc caaccaaaac
     1261 tggtcggtaa gcctaggagg tatatctgcc gtttttacgg gcaataacca cggtttactc
     1321 tgttacgatg gtgccgaatg gctgcttaag ccagttcccg ggaaatccat tattcgtgcc
     1381 gtgctctacg atagcagcag ggtttacgtc ggcttgctcg atgaattcgg ctacttcgat
     1441 attgtcaacg gaaagccaac catttaccat tgcctgtcaa ccacggtaag caacaacctt
     1501 aaaaatcagg agtactggcg gatagccaag caccacggga aaatatattt tcagagtttc
     1561 ggctcacttt acgtttacga tggacgggag gtttcctccg ttcctcttga ttttccgata
     1621 cttctgctta ttaaggcaaa cgatgagctt ttcacacagc gaattggtgg cgggttatac
     1681 cgcattaccg aaaatggtct taagctgttg gaaggcagcg agatatttgc agataccgaa
     1741 attaaggctg cccttaccca tcaaaacgac atcctatttt gcaccaactc catgggcatt
     1801 tacaagttca acgggttctc ctttgaaccc tggcacaccg ccgtggatgc cgaactcaaa
     1861 acctacaacc tgaacaccgc tgttgaaacc caacattact attgcattgg caccattcta
     1921 aagggcgtgc tggttctgga tcacaacggg cagctggttt accagctgga ctccaagcat
     1981 ctgcagaaca gcaccattct ttcgctcgag aaggataaaa tgggcaacat ctgggttggt
     2041 aaggataagg gtataagcta tgtggctttc aactcacccg tgagcatcca cattgacgac
     2101 accgataaat tctcgtgcta ctccggcgtt ctggttggct catacctcta cctgggaacc
     2161 aatcagggaa tattccgcta ctccttctcc agcgaaaaca tgctcaccgg caaaacgctg
     2221 gttaccggtt cgcagggcca ggtttggttt attaagctga ttgacgggaa gctttactgc
     2281 gggctaaacg acggcaccta cctgcttgaa aacgacaagc tggagaagat aggctttgta
     2341 agcggcgggt acaacctcga aaaattcagc aaggaaaagg atttgctcat ccaaagcacc
     2401 tacaacaacc tggtaaccta ccgaaataac aatgggaggt gggaacagaa caaaatcctt
     2461 cagggattct catcgcccag ccgatttctt caggttgact ttcagcagaa tatctgggtg
     2521 gggcattcca ttgtgggggt aaaccggctg caaaccaaca gcaatgttga cagcatcatc
     2581 agcatcgaga acattggcca ggagcatggc ctgaattttc aaaccaaccg catctacaaa
     2641 atcgaaaata gaattgtaat tccaacgggt tcgggatttt tgaggtggaa cgacctgaat
     2701 gagcggttcg agcccttcgg cgagctcaac gcccagcttc agggtttcga gaaggctcac
     2761 gccgttgcca gcctccccga cgacaagtac tggctgatta aggacgacga atgggggctt
     2821 ttcgaaatac gctttggcaa ggccaacctg ctttaccggg ttattcccga catgtttaac
     2881 atggagcttg ttgaggagaa cgagaagatc atccagctaa acgacagcct tcagctggtt
     2941 tgcctcgata atggttttgc catactcaac atacttcagc tcaacaggct gcctgaggtc
     3001 aaccttcccc ccaacatcaa cgacgtgcga ttctggagga atgaaaagga cagcgccagc
     3061 atcaagccc
//
LOCUS       DAOOQX010000179         3066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_38320_length_3066_cov_3.275988, whole genome shotgun sequence.
ACCESSION   DAOOQX010000179 DAOOQX010000000
VERSION     DAOOQX010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38320_length_3066_cov_3.275988"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PLX53_03850"
     CDS             complement(<1..993)
                     /locus_tag="PLX53_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOY72245.1"
                     /translation="MKEFVTACPRNCYSTCSFIVQVEKDKVVGIKPLSANKATPTGVC
                     LKGLSYVERANSPDRILYPYKKNGSGDFIRISWDEALSTIAGKLRYFKKTFGSQSVLF
                     YAASGMSGLINEFSGRFWREIYGGVTTTYGNLCWPAGLEATRLTLGENKHNAPWDLEN
                     ARLIVLWGKNPAESNIHEMIPLSTALDKGAKLAVVDPRRTSSSQRADLLVQPLPSTDA
                     ALALAVANILIKNSWIDEDFISSYVNGFEEFKAKAMSCTPEWASSICDISVESIEKLA
                     WLIGNVKPMTIIPGYGMQRYTNGGQTIRAILALSVITGNIGKPGACWHYANLQSY"
     gene            complement(1055..2401)
                     /locus_tag="PLX53_03855"
     CDS             complement(1055..2401)
                     /locus_tag="PLX53_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72246.1"
                     /translation="MIIKKGELQGSKISIYKNGNLERQMPVSSKGVLELTLDYDCDYI
                     FSFEKEGYISKKIAIDTRIPDKLKESFDKVTAFDVELFKQSENEVKEYKNPVARIQFS
                     SQISDFVFDTDYSLRVQQEIAQANKDLVEERKKQESLDLAKKEEEKRQQLLAEAQKRV
                     EAEAQKKAAEEKARQVEAEQLKKEAEERRLAEAKAKEEALKREEELRLKAREEARIKA
                     EQEALRIAEQKRIEEEKLKQEQERIRQEEEAKRKAAEEARIKEEQRIREEAERIRVAE
                     EAKKKADEEARLKELEQQKKEAEERAIADAKLKEEQRRIELEKAQKEAERKKFEEEER
                     LKAEQQQKLLFEEQKKRDKELRLKLVAEKMSAKIGTEDAERVVTLLTPEDVTKKYTRE
                     RTDEVIRLPRIVITRTVYNRNDKITFYTRVDHEYGAIFYFKDGYIISEEQYKAETK"
     gene            2725..>3066
                     /locus_tag="PLX53_03860"
     CDS             2725..>3066
                     /locus_tag="PLX53_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HOY72247.1"
                     /translation="MPMCFSINLNIEKYALEKSYHAKFSASYGYTPFYFASAFENPMV
                     PVIANRDSSLIVPMVWGLIPHWVTTPEQANLIRSKTYNARIETIHEKPSFRYAARSNR
                     CIIPVTGFFEWQ"
BASE COUNT          730 a          733 c          593 g         1010 t
ORIGIN      
        1 gtagctttga agatttgcat agtgccaaca agcgccgggt ttaccaatat ttcctgtaat
       61 tacagagagt gcaagtattg ccctgatggt ttgccctcca ttggtgtagc gctgcattcc
      121 gtatccggga ataatggtca taggcttaac attccctatt agccatgcca gcttctcaat
      181 ggattctact gagatgtcgc aaattgaaga agcccattcg ggtgtgcagc tcatcgcttt
      241 tgctttaaac tcctcaaatc cattcacgta gctactaata aaatcttcgt caatccagct
      301 gttctttatg aggatattag ccacagctag agccaaagca gcatcggtgg atggaagcgg
      361 ttgaaccagc aggtctgctc gctgggatga acttgttcgg cggggatcaa ccacagcaag
      421 ctttgccccc ttgtcgagag cagtgcttaa agggatcatc tcgtggatgt tgctttcggc
      481 tggatttttg ccccagagta caattagcct ggcattttcc aaatcccatg gcgcgttgtg
      541 cttgttctcg cccaaagtta accgtgtggc ttcaagccca gctggccagc ataaatttcc
      601 ataggtggta gttacacctc cgtatatttc gcgccagaac cttccgctaa actcgtttat
      661 aagtcccgac ataccgctgg cggcgtaaaa gagaacactt tgcgaaccaa aggttttctt
      721 gaaatagcgg agcttccctg caatagtcga aagtgcttca tcccagctaa ttctgataaa
      781 atcaccactg ccatttttct tgtagggata aagtatccta tcgggagaat ttgccctttc
      841 aacgtagctt aaccctttaa gacatacccc cgttggggtt gctttgtttg cagataacgg
      901 tttgatgcca acaaccttgt ctttttcaac ttgaacaatg aagctgcaag tactgtagca
      961 gttccgtggg catgctgtaa caaactcctt cacgaattat tatagttaca atctaaaaaa
     1021 tgggaactta cttaccggtt gagaatgaat tatgctactt tgtttcggct ttatactgct
     1081 cttctgatat aatgtagccg tccttaaagt aaaatattgc gccatactca tggtcaacgc
     1141 gggtgtaaaa ggtaatctta tcatttcggt tgtaaaccgt gcgggtaatc acaatccttg
     1201 ggagacgaat tacctcatcg gttcgttctc gggtatattt cttagtaaca tcttcggggg
     1261 taagaagggt taccacacgt tcggcatcct ctgttccgat tttagcactc attttttcgg
     1321 caaccagctt caagcgcagc tccttatccc tctttttctg ctcttcaaaa agcagctttt
     1381 gttgctgttc ggcttttaaa cgctcctcct cctcaaactt ttttctttca gcttcctttt
     1441 gtgccttttc cagctcaatc cttcgttgtt cttcctttag cttagcatca gcaatggcac
     1501 gctcctcggc ttcctttttc tgctgctcca actccttcag tctggcctcc tcatcggctt
     1561 tctttttggc ctcttcggct acccgaatgc gctctgcctc ctcgcgtatg cgttgttcct
     1621 ccttaatacg ggcttcctcg gcggctttcc tttttgcctc ctcctcctgc ctaatgcgct
     1681 cctgctcctg ttttaacttt tcctcctcaa ttcttttctg ttcggcaata cgcagggctt
     1741 cctgttcggc tttaatcctg gcttcttccc gtgcttttaa ccttaactcc tcctcacgtt
     1801 ttaatgcttc ttccttagct ttggcttctg ctaagcggcg ttcctccgct tcctttttca
     1861 actgttcggc ttcaacttga cgtgcctttt cttctgctgc ctttttttgg gcttctgctt
     1921 ccactctctt ttgagcctcg gctaaaagct gctgacgttt ttcctcttct tttttagcca
     1981 aatccaaact ttcctgcttt ttacgttcct ccaccaagtc cttgtttgcc tgggcaattt
     2041 cctgttgaac gcgtaaggag tagtcggtat cgaatacaaa atccgaaatt tgggaagaaa
     2101 attgaatccg cgctaccgga tttttgtact ccttaacttc attctcactt tgcttgaaaa
     2161 gttcaacatc gaatgcagta accttatcaa aactttcttt tagcttatcg ggtattcggg
     2221 tatcaatggc gatttttttg gaaatatatc cttccttttc aaaggaaaaa atgtagtcgc
     2281 agtcgtaatc gagagtcagt tcaagtacac ccttactgct aacaggcatt tgcctttcaa
     2341 gattgccatt tttgtatatt gatatcttag acccttgtaa ttcgcccttt ttaataatca
     2401 atcgggcaga tatttgtaag ccgttttgtg aaagcaatat gcttggaatt gctactaagc
     2461 atagggcaag ccatactatg agtatggttg atttggcctt ttgtttcaat tccgtaagca
     2521 ttaacccaaa ttttctaaca aaaaaagtaa aaaaatttga tattactacg aaaatttgag
     2581 caattctgtt cgcttcatcg gcatgactcc atagcatggc aagtacctgc ttaagtattt
     2641 tcgtagtaaa tgcaatcgta aacctgattt cagaaatatt cgtttacttt tgcattaaag
     2701 gtagaaaatt gggtgattga aaaaatgcct atgtgctttt ccataaatct aaatattgag
     2761 aagtatgcgt tggagaaaag ctaccatgcg aaatttagtg cttcgtatgg atatactcca
     2821 ttttactttg cctcagcctt tgagaatcca atggttcccg taatagccaa tcgggatagt
     2881 agccttattg tccctatggt ttggggatta attccccact gggttaccac accagaacaa
     2941 gccaacctga ttagaagtaa aacctataac gctagaattg aaacaataca tgaaaaacct
     3001 tcgttccgtt atgctgcaag gtctaacagg tgcattatac ccgttaccgg atttttcgaa
     3061 tggcaa
//
LOCUS       DAOOQX010000180         3057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_38486_length_3057_cov_2.936043, whole genome shotgun sequence.
ACCESSION   DAOOQX010000180 DAOOQX010000000
VERSION     DAOOQX010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38486_length_3057_cov_2.936043"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="PLX53_03865"
     CDS             complement(<1..1431)
                     /locus_tag="PLX53_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOY72248.1"
                     /translation="MLSSLEPNNLWKFFEEICKIPRPSKKEKLVIAYLQNFAREHGLK
                     VRTDHAGNVLVEKPAYPGFEKLKPVVLQCHMDMVCEKNSDVAHNFQTDPIVPGIDGEW
                     VKANGTTLGADDGIGIAAQLAILASTEIKHGPLECLFTVDEETGLTGANELEKNFFSG
                     KILINLDSEDEGELFIGCAGGVDTLATFAYDTEKVPTNSTAFRLTVKGLKGGHSGDDI
                     DKGLGNSIKIINRFLWRGVNSYDLRLSMLDGGNLRNAIPREAYAIFTIHEEDRAALRK
                     EFTLIKNDILAELHDVEPDLSITFENADLPPYVVDEPTQYDLLNALYACPHGVISMSR
                     QLKGLVETSTNLASVKFIQDNQILVTTSQRSSVESAKKDISNMVESVFRLANARIQHS
                     DGYPGWTPNTNSEILGITRNSYIRLFGVEPVVRAIHAGLECGLFLEKYPELDMISFGP
                     TIKAAHSPYERLNIPSTMKFWKLLLDV"
     gene            1673..2512
                     /gene="cysQ"
                     /locus_tag="PLX53_03870"
     CDS             1673..2512
                     /gene="cysQ"
                     /locus_tag="PLX53_03870"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HOY72249.1"
                     /translation="MIEQPEIINLLVTAINTSVRAGSKIMEVYNSDDFQVNLKSDRTP
                     LTLADRVAHEEIKNTLYKTLIPVLSEEGRSIHYVERKGWEHFWIVDPLDGTKEFIKRN
                     GEFTVNIALMYEGFPILGVIYVPVTECLYYSIHPEGSFKVTGIKPTMEVNLTYSDVCS
                     GAKRLPLSQNHSGIVVVESRSHNSPETLEFISNLDKKYGGITRISCGSSLKMCMVAEG
                     IADIYPRLSLSSEWDTAAGQAIVEGAGFQVISFDSGDRMSYNKEELINPWFIVRNPAR
                     FEV"
     gene            complement(2509..>3057)
                     /locus_tag="PLX53_03875"
     CDS             complement(2509..>3057)
                     /locus_tag="PLX53_03875"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOY72250.1"
                     /translation="VAGAFMSLMAINLLRDAVGVNNITEPQKISTFLYNELIRKKASI
                     FDSNLIIGLDFIVCSYSPKSQVLCYSGNHLNFYLVREGEVNKLKVERNIDRQSATLIF
                     AQSSIKLKKNDRLYFTTDGFADQISETKRKKIGNSDFQNLLLSVGNYPIKQHSELLAE
                     YFSKWKGTYEQVDDVLVLGLEV"
BASE COUNT          784 a          649 c          733 g          891 t
ORIGIN      
        1 cacatccagc agaagcttcc agaacttcat ggttgaagga atgttaagcc tttcgtaggg
       61 agagtgtgcc gctttaatgg ttgggccaaa cgaaatcata tccagctcgg gatatttctc
      121 aagaaataag ccgcattcca gaccggcatg gattgccctt acaaccggtt ctactccaaa
      181 aagcctgatg tacgagttac gggtaatgcc cagtatttca gagttggtgt tgggagtcca
      241 tcctgggtag ccatcggaat gctgaattcg ggcattggcc agccggaaaa cactttcaac
      301 catgttggaa atatcctttt tggccgactc cactgagctg cgctggcttg tggttacaag
      361 tatctggttg tcctgtataa atttcaccga agccaggttg gtagatgttt caaccaaccc
      421 tttaagctgc ctactcatcg aaataacccc atgtgggcag gcatacagcg cgtttagcag
      481 gtcgtattgg gtgggctcat caaccacata gggaggtagg tcggcgtttt caaaggttat
      541 ggataaatcg ggttcaacat catggagctc ggctagaatg tcatttttga tgagtgtaaa
      601 ttcttttcgt agggcagccc tatcctcctc gtgtatggta aaaattgcat aggcctcacg
      661 gggaatggca tttctaaggt ttccaccatc tagcatggat agccgtaggt cgtagctgtt
      721 aaccccccgc cagagaaaac ggttgataat cttaatagag ttgcccaagc ccttgtcaat
      781 gtcgtcacct gaatgccctc cctttagccc cttcacggtt agcctgaaag cggtagagtt
      841 ggtgggtact ttttcagtgt cgtaggcaaa ggtagccaga gtatcaactc caccggcaca
      901 cccaataaat agttctccct catcctccga gtcgaggtta atcagaattt taccgctaaa
      961 aaaattcttt tcgagctcgt tggctcctgt caaacctgtt tcctcgtcaa cagtaaaaag
     1021 gcattccagc ggtccatgct taatttcggt tgatgccagt atggcaagct gggcggctat
     1081 ccctattccg tcgtcggcgc ccaaggtggt tccattggcc ttaacccatt ctccgtcaat
     1141 gcctggcaca attgggtcgg tttgaaagtt atgcgccaca tcgctatttt tttcgcaaac
     1201 catgtccatg tggcactgca gcactacggg cttgagtttt tcaaatccgg gataggcagg
     1261 tttttctact agcacgtttc cggcatggtc ggttcttact ttaaggccat gctccctggc
     1321 aaaattttgc aggtaggcaa tgaccagctt ttcctttttt gatgggcggg gaattttaca
     1381 gatttcctca aagaatttcc aaaggttgtt gggttcaagc gagcttaaca tggtgatatg
     1441 ggttaaaagt taggtattgt tatgtcttaa aattttgggt aaacataaca atttttccaa
     1501 ttttgaggaa ccttagcaaa catattgtaa aacatcaatt ggtaagcggt ggaaggaagt
     1561 ttctttatgc ttatttttta atgaataaaa atgttaaatg gggttggtgg ggaagggtat
     1621 attttttatt attcaaggaa agtgagctac ctttgcagca agcaactagg tcatgattga
     1681 acagccagag atcataaacc tgctggttac ggctataaat accagcgtaa gagcaggttc
     1741 aaaaatcatg gaggtttata actccgatga tttccaggtt aacctcaagt ctgatagaac
     1801 acccctcacc ttggccgaca gggtggctca cgaagaaata aagaatacgt tgtacaaaac
     1861 tttaataccg gttcttagcg aggagggtag aagcattcac tatgtggagc gaaagggttg
     1921 ggagcacttt tggattgtag atcctctgga tgggacaaag gagtttatca agcgcaacgg
     1981 agagtttact gtaaatattg ccttaatgta tgaggggttt cctatcctgg gcgttatcta
     2041 tgttcctgtt accgagtgcc tttactattc cattcacccc gagggttcgt ttaaggttac
     2101 aggtatcaag ccaacaatgg aggtgaattt aacctactct gatgtgtgct ccggggccaa
     2161 gcgcctacca ctaagccaaa atcatagcgg tattgttgtg gttgaaagcc ggtcgcacaa
     2221 ctcgcccgaa acgctggaat tcatttcgaa cctcgataaa aagtacggtg gcattacccg
     2281 tatttcgtgt gggtcttctc ttaaaatgtg tatggtagcc gaagggatag ccgatattta
     2341 tccgcgttta tcgctttcct ctgagtggga tactgctgct ggtcaggcta tagttgaggg
     2401 tgctggtttt caagtaattt ccttcgattc gggagataga atgtcctaca ataaagaaga
     2461 gctgataaat ccctggttta tcgttcgtaa tccagcccgc tttgaggttt aaacctctaa
     2521 ccccagaact aatacgtcgt caacctgttc gtaggtaccc ttccattttg agaagtactc
     2581 agcgagtaac tcagaatgct gttttatggg atagttacct actgagagta gaagattctg
     2641 gaaatcggaa tttccaattt ttttcctttt ggtttcgcta atctggtcgg caaagccatc
     2701 ggtagtgaag taaagcctgt cattcttttt gagcttaatg gatgactggg caaaaatcag
     2761 ggtggccgat tgtctgtcaa tatttctctc aacctttagc ttgttaacct ccccctctcg
     2821 caccaggtag aagttaaggt gatttcctga gtagcagagt acttggcttt taggggagta
     2881 gctgcaaaca ataaagtcga gtccgatgat caggttggag tcaaaaatag aggctttttt
     2941 tctgattagc tcgttgtaaa ggaaggtaga gattttctgt ggctccgtaa tgttgttcac
     3001 cccaacggca tccctaagca gatttatagc catcaggctc atgaaggcac cggctac
//
LOCUS       DAOOQX010000181         3051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_38597_length_3051_cov_3.214286, whole genome shotgun sequence.
ACCESSION   DAOOQX010000181 DAOOQX010000000
VERSION     DAOOQX010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38597_length_3051_cov_3.214286"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="PLX53_03880"
     CDS             <1..1315
                     /locus_tag="PLX53_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOY72251.1"
                     /translation="KLHYDGRTSEGRLTGKEVKMVDKDFTLVTDALDFNSKTSLAFYT
                     TGGVATSNDSSTLTSIKGYYNNNDNLISFANKVVLKSPDGNAFTDSLSYHTKNEVAYF
                     FGPTFIYDGENFIYCEKGWHNRKLQQSNFQNNAYLISNSQKLFGENIYYDKQNGYARG
                     IGNVVMVDTVNRTYIYGQKASYWQEKEEAEITEQPYMMMIDDSDSLFLRTDKLLMITA
                     KTGDNQKTDSSYRLVKAIGRVIYYRHDIQGVCDSMIYNTRDSVLEMYGNPVMWNEANQ
                     MTADFIRIHISNKKISQADFLGNAFISSREDSAYFSQMRGKTILARFSDGQLTRMDIT
                     GNGQVVYFVRDKGQITMVNRAEGSNVDVHIKNNKVSRVVFKQKPTSNLIPIEQVEPED
                     MTLKGFKWLGQLRPESKYSVIPTSLTLVPLEKGTQRRMEMMANFK"
     gene            complement(1415..1822)
                     /locus_tag="PLX53_03885"
     CDS             complement(1415..1822)
                     /locus_tag="PLX53_03885"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOY72252.1"
                     /translation="MELISWESNYSVKVGSIDADHKKLVEILNSLFMAMSKGEGSKII
                     SDLVKELQRYTIYHFSREESFFRITGYPNTQKHIEEHKQFIGKVKEYKEKIDKGNNNF
                     SSEMLAYLKDWLLNHILVTDKAYSEHFVKHGIA"
     gene            complement(2048..2455)
                     /locus_tag="PLX53_03890"
     CDS             complement(2048..2455)
                     /locus_tag="PLX53_03890"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOY72253.1"
                     /translation="MELFSWKNDYSVKVGSIDSDHQKLFDIINSLFTAMSKGEGRQVI
                     SGLVKELQSYTIYHFNREETFFRVTSYPNAANHLEQHKIFVSKVKEYKEKIDKGNNNF
                     SPEMLTYLKDWLLNHILVTDKAYSDHFTKNGIV"
     gene            complement(2521..>3051)
                     /locus_tag="PLX53_03895"
     CDS             complement(2521..>3051)
                     /locus_tag="PLX53_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOY72254.1"
                     /translation="LDVMMPDMDGIETCQELRANPLTKDCLIAFLTARSEDYSQIAGF
                     DAGGDDYISKPIKPKVLISRIKALLKRSGSLEEAPEPTPEIPEEGIYINRERYLVYLD
                     GRELVLPKKEFELLALLQSKPQKVFTRDEIYQAIWGDDIVVGDRTIDVHIRKLREKLG
                     DQYIRTVKGVGYKFTV"
BASE COUNT          902 a          700 c          626 g          823 t
ORIGIN      
        1 caagctgcac tatgacggga gaacctccga gggaaggcta accggcaagg aggttaaaat
       61 ggttgacaaa gattttacac tggttaccga cgccctggat ttcaactcga aaacaagcct
      121 ggctttctac accaccggag gagtggcaac ctccaacgac tcatccacct taaccagcat
      181 aaaaggctac tacaacaaca acgacaacct gatatccttt gccaataaag ttgtcctaaa
      241 aagtcccgat ggtaatgcct ttactgactc cctatcgtac cacaccaaaa atgaggttgc
      301 ctactttttt ggaccaacct ttatctatga cggagaaaac tttatatact gcgaaaaagg
      361 ctggcataac cgaaaactac aacaatcgaa cttccaaaat aacgcctatc tcataagcaa
      421 ctctcaaaaa ctctttggcg aaaatattta ctacgataag caaaatgggt atgcccgtgg
      481 aataggaaat gttgtgatgg ttgacacggt aaaccgcacc tacatttacg gccaaaaggc
      541 cagctactgg caggaaaaag aggaagccga aattaccgag caaccctaca tgatgatgat
      601 tgatgattcc gacagcctat tccttcgcac cgacaagctg ctcatgatta cagccaaaac
      661 aggtgataat cagaaaaccg actcatccta tagattggta aaggcaatag gcagggtgat
      721 atactaccgc cacgatattc agggcgtttg cgattccatg atctacaaca cccgcgactc
      781 tgttttggag atgtatggca acccggttat gtggaacgag gcaaaccaga tgactgccga
      841 tttcatccgt atccatataa gcaacaaaaa gataagccag gctgactttt tgggcaatgc
      901 cttcatatca tcccgtgagg actccgcata ctttagccag atgaggggta aaaccatttt
      961 agccaggttt tccgatggcc agcttacccg aatggatatt acgggaaacg gacaggtggt
     1021 ttacttcgtt agggacaagg gtcaaatcac catggtgaac agggctgaag gttctaatgt
     1081 tgacgtccac attaagaaca ataaggtcag ccgggtggta ttcaagcaga agccaacttc
     1141 aaacctcatt cctattgaac aagtagaacc cgaagatatg accctaaaag gatttaagtg
     1201 gcttggccag cttagaccgg aaagcaagta ttcggttata cccaccagct taaccctcgt
     1261 tccacttgag aagggaaccc aacgaagaat ggaaatgatg gccaacttca agtaggacga
     1321 gatattgata gaagtgatat ttgccctaaa ggcaaacact atccaagcct atttggaagt
     1381 catttcgtcc tccaaacttc ttcatatcag caattcaggc tattccgtgt tttacaaaat
     1441 gctccgagta ggccttgtcg gtaacaagga tatggttaag caaccaatcc tttaggtagg
     1501 ccaacatttc ggacgagaag ttattgtttc ccttgtcaat tttttcctta tactctttca
     1561 ccttaccaat aaactgctta tgctcctcaa tatgtttttg ggtattcggg tagccggtaa
     1621 tcctaaaaaa agactcttct ctgctaaaat ggtaaattgt ataacgctga agttccttaa
     1681 ctaagtcgga gataatctta ctcccctcac ccttcgacat ggccataaaa agcgagttga
     1741 gtatttccac aagtttctta tgatcggcat ctatgctgcc aaccttaacg ctgtagttgc
     1801 tttcccacga aattagctcc atagcaaata ttctttttga aaatagttga aagaaaggta
     1861 gaaaatatca aattataacc gtaatgctac taatataaaa ttaccactcc tggttgaaat
     1921 tagaagaatc tgaaatattg atacactgtt caaagtgtat aatccttccc attatcagcc
     1981 taaaattagc tgtggtaaga aaaattgtag tttaaagaat accccctccc ttgttggaga
     2041 gtggattcta tacaatgccg ttctttgtga agtgatcaga gtaggccttg tcggtaacaa
     2101 ggatatggtt aagcaaccaa tcctttaggt aggtcaacat ttcgggcgaa aagttattgt
     2161 ttcccttgtc aattttttcc ttatactctt tcaccttgct aacaaaaatt ttatgctgct
     2221 caaggtggtt ggcagcattg gggtagctgg taaccctgaa aaatgtctcc tcccggttga
     2281 aatggtaaat ggtgtagctc tgcagctctt taaccaaacc ggagataacc tgcctgccct
     2341 cacccttcga catggcagta aacagtgagt taataatgtc gaacagcttc tgatgatcgg
     2401 agtcaatgct tccaacctta acgctgtagt cgtttttcca cgaaaaaagc tccatatagg
     2461 taaaattaat ttaggttgct tccaagaaat ttcccttata gctacctggg gagatgcagc
     2521 ctagacggtg aacttgtatc caacaccctt taccgttcta atgtattggt ctccaagctt
     2581 ctcgcgcagc ttgcgaatat gcacgtcgat agttctgtca cccacaacaa tgtcgtcgcc
     2641 ccaaattgcc tggtaaattt catcacgggt aaaaaccttt tggggtttac tttgaagcag
     2701 ggccagcagc tcgaactcct tttttggaag aaccagctcc ctgccgtcga ggtaaacaag
     2761 ataccgttcc ctgttgatgt agattccctc ctcaggaatt tcgggtgttg gttcaggtgc
     2821 ttcctccagc gagccagatc gtttaagcag ggccttaata cggctaatta gcactttggg
     2881 cttaattggc tttgaaatgt agtcgtcgcc ccctgcgtca aagcctgcta tctggctata
     2941 gtcctcgcta cgtgcagtta aaaaagcaat gaggcagtct tttgttaacg gatttgcccg
     3001 taactcctgg caagtttcaa ttccatccat atcgggcatc atgacatcaa g
//
LOCUS       DAOOQX010000182         3050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_38617_length_3050_cov_2.694825, whole genome shotgun sequence.
ACCESSION   DAOOQX010000182 DAOOQX010000000
VERSION     DAOOQX010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38617_length_3050_cov_2.694825"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..2515
                     /locus_tag="PLX53_03900"
     CDS             245..2515
                     /locus_tag="PLX53_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOY72255.1"
                     /translation="MERAYSKPFVVRITILGFIAGLAVILLAFFIAAVTGGYSFNFSG
                     IVEMHRIMPVLYFLDIFPLLIALASFYISRYISRIRMESESSSEETIHRQRKLYHFVE
                     KLQEGDESVEYIPDEGDALGKAIVNLRNNISQSRKEEEIRRKEDEQRRWSAEGMARFG
                     EILRLNNDNVEELSFQIIRNLVNYVGANQAAFYLLEDDDEADVHFRMTACYAYERRKY
                     ADRIVPWGDGLVGACALEKETIFLKKVPDSYLNITSGLGKANPRCLVLIPLKLNDQVH
                     GVLEVASFNIFEKHQLEFMEKIAESVATTISTVKINLRTAKLLRESREQAEELALKEE
                     QMRQNMEELQATQEEAARQSEKFISFSNSVNHTMIRADFDVKGTLTYANTKFMQKLEY
                     SSTAEVEGKPLDIFLDRRDRVWFEEVWKKLSKGGKHFEGNMKLATKTGKELWILATFT
                     CMRNTMGDVDKILFLALDASGQRAKNLELEGQVNAFSRSVVKADISPTGDMIAVNDMY
                     IETLGLPYEVAMKKNIFETVSPVDRQNVEDAWDDIIHGNPFDGKLRVNTGNNDEKWLQ
                     VSLVPVNDTNGEIAKVLLIAYDITSEVHLAIEVQQKEETIRKQEELIQQKEVEQNKKL
                     RETREEITNQYRETEKIKVRNEKLLEELSEAIVTTDHDGLILFMNKAAENLFTVKHDE
                     VANQNVRILFSDTVKDNFVTTYLDPARKKIVGQRQMVTLNPRKGKEVNATMVVYEVKT
                     GREITYTAFFQPTTNS"
     gene            complement(2525..>3050)
                     /locus_tag="PLX53_03905"
     CDS             complement(2525..>3050)
                     /locus_tag="PLX53_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72256.1"
                     /translation="GIPVLYLSVPLLVPLLVNNSELYASFNLLPLLFAGMVSRIWYYY
                     LVAPIYYFKQTKVMPLVFGFAAIFQIASTYILIQLNGIDGAVWANLLTKLFQVFLLYL
                     FVRRFYTLRVNVKKLIVFPAVYMMLLISAEIFLKPVNIYLLNLIHIVIIGVLAYSFFR
                     NELKWENIRTALKK"
BASE COUNT          956 a          576 c          724 g          794 t
ORIGIN      
        1 tatttttttc aattttgttg atacttcaaa atatcaggta gttctggtaa tacgttctgt
       61 tgattttagt ttgtttgcta tgaattttgt tcatttgcac ttatccggta tagctgggct
      121 gactttggga agaataggta tcggataggg cctcgatttt ggctggtaac acgctaaatt
      181 tggaatcgaa tttttccatt caattgaaaa aatacctact tttaaaatca aaaataatac
      241 tgccatggag cgagcatatt caaaaccctt tgtagtcaga ataaccattt taggatttat
      301 tgccggttta gctgttattc tgcttgcatt ttttattgca gcggttactg gagggtattc
      361 tttcaacttc agcggcattg ttgaaatgca tcgcattatg ccggttttat attttttgga
      421 tattttccca ttgcttatag ctctggcatc attctacatt tcacgctaca tctcacgtat
      481 caggatggag tccgaaagta gctcggaaga aacgattcat aggcagcgga agctatacca
      541 ttttgttgaa aaacttcagg agggagacga aagcgttgag tatatcccgg atgagggtga
      601 tgcgctgggc aaggcaatag taaatcttcg caacaacatt agccaaagcc gtaaggagga
      661 ggagataagg cgaaaagagg atgagcagcg ccgatggagc gctgagggaa tggcccggtt
      721 cggcgaaata ctcaggttaa ataatgataa cgttgaggag ctatcatttc aaataatcag
      781 gaacctggta aactatgttg gtgcaaacca ggcagcattc taccttcttg aggatgacga
      841 tgaggctgat gttcattttc gtatgactgc ctgctacgcc tacgagcgtc gaaagtatgc
      901 tgacagaatt gttccctggg gcgatggttt ggttggtgca tgtgccctcg aaaaggaaac
      961 tatttttttg aagaaggtac cggacagcta cctgaacatt acatccggac taggtaaggc
     1021 aaacccacgt tgtctggttc tcatacccct taagctaaac gatcaggttc atggcgtgct
     1081 ggaggttgct tcgttcaaca tattcgagaa gcaccagctg gagtttatgg aaaagattgc
     1141 cgaaagcgtg gcaactacca tttccacggt aaagataaac ctacgaacgg ccaagttgct
     1201 gagggaatca cgcgaacagg ccgaagaact tgccctaaag gaggagcaaa tgcgtcagaa
     1261 catggaggag ttgcaagcca cccaggagga agctgcccgc caatcggaaa agttcatcag
     1321 cttctccaac tcggttaacc acaccatgat ccgggccgat ttcgacgtta aggggaccct
     1381 aacctacgcc aacaccaaat tcatgcagaa gctggaatac tcctcaactg ccgaagttga
     1441 aggaaaacct ttggatattt ttttagatag acgggatagg gtatggtttg aagaggtgtg
     1501 gaaaaagctt agcaaaggtg gtaaacactt tgagggtaac atgaagctag ctaccaaaac
     1561 cggtaaggag ctatggattc tggccacatt tacctgcatg agaaatacaa tgggtgatgt
     1621 tgataaaatc ctctttttag ctcttgatgc ttctggacaa agagcgaaaa accttgagct
     1681 ggaaggtcag gttaacgcct ttagccgatc tgtagttaaa gccgatatca gcccaaccgg
     1741 agatatgata gcagtaaacg atatgtacat cgaaacgctg ggactacctt acgaggtggc
     1801 catgaaaaaa aacatatttg aaaccgtttc accggttgac cgccaaaatg ttgaggatgc
     1861 atgggatgat attattcatg gtaatccatt tgatggaaag ctaagggtaa atacgggaaa
     1921 taatgatgag aagtggctac aggtatccct cgtcccggtt aacgatacaa atggtgaaat
     1981 tgccaaggtt ttactgatag cttatgacat taccagtgaa gtgcaccttg ccattgaagt
     2041 tcagcagaaa gaggaaacca ttagaaaaca ggaagagtta atccaacaaa aggaagtaga
     2101 gcaaaataag aaattacgcg aaacgcgtga agaaattact aatcaataca gggaaacgga
     2161 gaaaattaaa gttcgtaacg aaaaacttct ggaagagctt tccgaagcta ttgtaaccac
     2221 cgaccacgat gggctaattc tgtttatgaa taaggctgct gagaatttat ttactgttaa
     2281 acacgatgag gtagcaaacc agaatgttcg aattctgttt tccgatactg taaaggacaa
     2341 ctttgttacg acatatttag accctgcaag gaaaaaaata gttgggcaac gccagatggt
     2401 aacactaaac cctcgaaagg gtaaagaggt taacgcaaca atggttgttt acgaagtaaa
     2461 aaccggtagg gaaattacct acacagcatt ctttcaacct acaaccaact cctagagagt
     2521 tgggctattt tttcaaagct gtccggatgt tttcccattt aagctcatta cggaaaaagg
     2581 aataggctaa caccccaatt attacaatgt gaatgaggtt gagcaggtaa atatttaccg
     2641 gctttaaaaa tatttctgcc gaaatgagta gcatcatgta aactgccggg aaaacaatta
     2701 gctttttaac atttaccctc agggtgtaga atctccttac aaaaagatat aaaaggaata
     2761 cctgaaaaag ttttgtgagc aggtttgccc atactgcacc gtcaataccg ttaagctgga
     2821 taagaatgta ggtgctggct atttgaaaaa ttgcggcaaa cccaaaaacc agtggcatta
     2881 ccttggtttg cttaaagtag taaattggcg ccacaaggta gtagtaccag atacggctca
     2941 ccatgccggc aaaaaggagg ggtaggaggt tgaatgaggc gtaaagttcg ctgttgttaa
     3001 ccagcaatgg aaccagcagg ggaaccgaga ggtagagtac tggtatccca
//
LOCUS       DAOOQX010000183         3031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_38950_length_3031_cov_4.074261, whole genome shotgun sequence.
ACCESSION   DAOOQX010000183 DAOOQX010000000
VERSION     DAOOQX010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38950_length_3031_cov_4.074261"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PLX53_03910"
     CDS             complement(<1..991)
                     /locus_tag="PLX53_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOY72257.1"
                     /translation="MKYLNSVITGTGSYIPSVKISNREFARNTFFKKDESAIETLVDE
                     VIAKFKDITGIEERRWVTDDLCASHIAAIAAEEAIATAGIDRETIDQIIVSHNFGDVL
                     QGTIQSDTIPSVASRVKHLLKIKNPACIPYDVIFGCPGWVQGVIQADSYIKSGLAKRC
                     LVIGAETLSRVIDKHDRDCMIFADGAGATIVEAVEADEKAGVIASSMMSHTEQEVFYL
                     SLGKSFHPDSDSRIRYIKMDGRKIYEYSLINVPGAMREAIEKSGINPSELKKIIIHQA
                     NEKMDEAIVARFIKQLKIQTPISDLMPMSIKELGNSSVATIPTLYDLILKGKVP"
     gene            complement(1111..1725)
                     /locus_tag="PLX53_03915"
     CDS             complement(1111..1725)
                     /locus_tag="PLX53_03915"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOY72258.1"
                     /translation="MNAVIVANGKFPEHPKPLRLIHEAKTLLCCDGSIEHLVKLGITP
                     TAIVGDLDSVSPGLRERFKNILFHDPDQNSNDLTKAVSWCAERNINSISIVGATGKRE
                     DHTVGNIGLLVRYARMGLQVEMVTDTGTIKPLLESAKLTSFPGQQISIFSPVNQTTIT
                     TNNLKYPIDNGQIPEWWMGTLNESLGSWFELNFSPGPLLVYQMY"
     gene            complement(1760..2383)
                     /gene="pnuC"
                     /locus_tag="PLX53_03920"
     CDS             complement(1760..2383)
                     /gene="pnuC"
                     /locus_tag="PLX53_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOY72259.1"
                     /translation="MSWLIGNYIELLGATIGLIYLYFEIKQKIWLWPLGIATSALYIY
                     IYFSSKFYADMGLQVYYLIISIYGWYHWLHGNVDNTSSSKPLPVTRITLRLLVVLTTI
                     SLLLFVILWFVLDRYTDSPVPVGDAFTTALSITATWMLTRKIIEHWWVWIVVDGVSMG
                     LYIYKGLYPTTVLFAFYTILSVVGYYQWKREITPSQVKTTAGSSAKE"
     gene            complement(2656..>3031)
                     /locus_tag="PLX53_03925"
     CDS             complement(2656..>3031)
                     /locus_tag="PLX53_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY72260.1"
                     /translation="GNTTISYSPSVVGSAKITVTPVKALYFSWIGKYVGKQYIDNTES
                     SSRSLDAYFVNNIVGEYKFSPKMAKSISLQVVVNNLFNLKYEANAWVYRTKFQSGDPE
                     YVSIGYYPQATRNFMLRLAVGF"
BASE COUNT          849 a          634 c          680 g          868 t
ORIGIN      
        1 tgggcacttt tccctttagt attaggtcgt aaagtgttgg aatggttgcc accgagctat
       61 tgcctaactc cttaatgctc attggcatga gatcggaaat gggggtttga atttttagct
      121 gtttgatgaa acgtgctaca atggcctcat ccatcttttc gttagcctga tgaatgataa
      181 tttttttaag ttcgcttggg ttaatccctg atttttcgat ggcttcgcgc atggcacctg
      241 gtacgtttat aagagaatac tcgtagattt tacgaccatc catttttatg taccgtatgc
      301 gggaatccga atcgggatgg aaggatttcc ctagtgaaag gtagaagact tcctgctcgg
      361 tgtgcgacat cattgaggag gctatgaccc cggctttttc gtcagcttca accgcctcaa
      421 caatggtagc accagcgcca tcagcaaaaa tcatgcagtc gcggtcgtgc ttgtcaatga
      481 ccctcgaaag ggtttcggca ccaatcacca ggcatcgttt ggccaaccct gatttaatat
      541 atgaatcggc ctgaatgacc ccttgaaccc aacccggaca accaaatata acatcgtaag
      601 gaatgcaagc aggattttta attttcaaca ggtgcttaac acgtgatgca acgctgggga
      661 tggtgtcgga ctgaatggta ccctgaagaa catccccaaa gttgtgcgaa actatgattt
      721 ggtcaatagt ttcgcggtca atgcccgcag tggcaatagc ttcttcagca gcaatggctg
      781 caatgtgcga ggcgcacaaa tcgtcggtta cccagcgccg ctcttcaata ccggtaatat
      841 ctttaaattt agcaataacc tcatcaacca gggtttcaat tgccgattca tcttttttaa
      901 agaaggtgtt acgggcaaat tcgcggttgc taatcttaac ggaagggatg tagctacctg
      961 ttccggtaat aacggaattt agatacttca tgatgtggag atttaattag tcccaccaca
     1021 aagctaacaa tatgcaatca aaaagcaatg atattgtaaa taaaatttat aatatattga
     1081 tattcagaaa ctagtatata ttacagttta ttagtacatt tgataaacga gaaggggacc
     1141 aggagaaaaa ttgagttcga accaactgcc cagcgattca ttcagcgtac ccatccacca
     1201 ctcgggaatc tggccattat caataggata tttaaggtta tttgtggtaa ttgtagtttg
     1261 gttaacggga gaaaagattg aaatttgctg tccgggaaag ctggttaatt tagctgattc
     1321 taaaagaggc ttgatagtgc cggtgtctgt gaccatttca acctgtaagc ccattctggc
     1381 atacctaaca agtagcccga tgtttcccac tgtgtgatcc tcacgttttc cagttgctcc
     1441 tacaatggaa attgagttga tgttacgctc agcacaccag gaaacagcct tggttaggtc
     1501 gttgctgttt tggtcggggt cgtgaaaaag tatgtttttg aaacgctccc tgagtccagg
     1561 gctaaccgaa tcaaggtcgc caactatggc ggtaggggtt ataccgagtt taacaagatg
     1621 ctctatggac ccatcgcagc aaagtaaggt tttagcttca tgtataagac gaagcggttt
     1681 agggtgttcc ggaaatttgc cgttggcaac aattacagcg ttcatagggc acttgtttga
     1741 gttatatgga ctagcaaatt tactcttttg cggaagagcc agcggtagtt tttacttgag
     1801 atggggtaat ttcccttttc cattggtagt accccacaac cgacaaaatg gtgtaaaaag
     1861 caaaaagcac ggtggttggg taaagtcctt tatagatgta gagtcccata gagaccccat
     1921 caactacaat ccaaacccac cagtgttcga taatttttcg tgtcaacatc caggtggcag
     1981 tgatgctgag cgccgttgta aaggcatcgc caaccggaac aggggaatcg gtgtacctgt
     2041 cgagcacaaa ccataaaata acgaatagta aaagcgaaat agtcgtaaga actactagta
     2101 gcctcaatgt tattcgggta accggcaggg gctttgagct ggaggtattg tcaacatttc
     2161 catgcagcca gtggtaccat ccgtatatac ttattatcaa atagtaaacc tgaagcccca
     2221 tgtcggcgta aaattttgat gaaaagtaga tgtatatgta aagagccgag gtggcaattc
     2281 ccaatggcca tagccaaatt ttctgcttta tttcgaagta tagataaatc aagccaatgg
     2341 tggcacccag aagttcgatg tagttaccta tcagccagct cattttattt cagggataaa
     2401 gaattgaaga tttcagattc aaaattcaag aacttgtacg cctgctggtt gattcaccct
     2461 tcggcaagct caaccggcaa acgggcaagt tccttctaaa gatttaggac aagcatttgg
     2521 cttctcacgc ttaatggttc aattaattca ttttctttca acttattcct aatctttttg
     2581 gcaagcaggg gcgggcttgc tgctgcccac ccctattatc atcgcatgat gctcttcatt
     2641 attgttgata taatttcaga acccaaccgc aagtcggagc atgaaatttc gggtagcctg
     2701 gggatagtag cctatgctga catactccgg atcgccactt tgaaattttg tgcggtacac
     2761 ccatgcgtta gcctcatatt tcaggttgaa aaggttatta actacaacct gcagtgaaat
     2821 gcttttggcc attttgggtg aaaatttata ctcaccaaca atgttgttta caaagtaagc
     2881 atccaaactt ctgctactgc tttcggtgtt atctatgtac tgcttgccca catacttacc
     2941 aatccatgaa aagtaaagtg ctttaaccgg ggtaaccgta attttagctg aacccaccac
     3001 ggagggggag taggaaatgg ttgtgttacc a
//
LOCUS       DAOOQX010000184         3022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_39121_length_3022_cov_2.929558, whole genome shotgun sequence.
ACCESSION   DAOOQX010000184 DAOOQX010000000
VERSION     DAOOQX010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39121_length_3022_cov_2.929558"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..636)
                     /locus_tag="PLX53_03930"
     CDS             complement(34..636)
                     /locus_tag="PLX53_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOY72261.1"
                     /translation="MEFGCAEVLFKKGEVVFREGTLATNTIYLKRGMVKVHMKGPSGD
                     KILKLIKAPTYLGLPTTFGDRINQYSATAISDAEVCFISLDTFKDFITKNGDFAYELI
                     MDLCRNELVEYRRCTSMSQKQIPGRVAEVLLCMSDDLFCSSTFEMPLNNSELADLIST
                     SRETVNRQLSEFIREGLISMTKRKIQILDAKRLGQISING"
     gene            887..1432
                     /locus_tag="PLX53_03935"
     CDS             887..1432
                     /locus_tag="PLX53_03935"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOY72262.1"
                     /translation="MEIDVEKQLEELKKKVGKLESGRKDKLTMVVFSGDMDKLLAAMI
                     IATGATAMDMEVSLFFTFWATAALRDPKKKVKGKNLVSKMFGFMLPKGAKKVKLSKLH
                     MAGMGKTMLENIMKKKNVASLDEMFKTAAELGVSINVCEMSMNLMGFKREELIDYPNL
                     KICGVGTFLSDAEESKIQLFI"
     gene            1456..1818
                     /locus_tag="PLX53_03940"
     CDS             1456..1818
                     /locus_tag="PLX53_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOY72263.1"
                     /translation="MREKLIVIVTEGPDNQEKATIPFVMATAAQSMDVDVTIILQAEG
                     ALLVKKGVSENVNASGFMPLKQLLDTFIELNGKLLVCTPCAKSRGISENDLVEGSKLI
                     AAGTVISESLSANAVLSY"
     gene            1841..2092
                     /locus_tag="PLX53_03945"
     CDS             1841..2092
                     /locus_tag="PLX53_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOY72264.1"
                     /translation="MNAEELKNLKVSQTVDARGTACPGPLLAAKKAIGEIESGEIMEV
                     LSSDEGTKHDIPKWCEKMEHEFLGIIEEDSFTRLFLKKA"
     gene            2181..2609
                     /locus_tag="PLX53_03950"
     CDS             2181..2609
                     /locus_tag="PLX53_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOY72265.1"
                     /translation="MLSETFSPKILVFSTDIISDPGIDQAGLRKLHYPPTVYVISLPC
                     SSGIKPRWIMRAFEKGFDGVFIAADGHECSYSPKCAEHTNNIIVESQKLMKENNINPK
                     RIRMAALCSVCAEPFVNHMGNFSKILSELGSIGTEKYNPS"
     gene            2734..>3022
                     /locus_tag="PLX53_03955"
     CDS             2734..>3022
                     /locus_tag="PLX53_03955"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY72266.1"
                     /translation="MPENKKTFDALVVGGGIAGQEAALSLANMDYKVLLVEKELSIGG
                     KMIQLSKVFPTLDCAACITTPKMSETARHPNIQLLLNTDIMEITKNNGLFNV"
BASE COUNT          963 a          594 c          643 g          822 t
ORIGIN      
        1 tagaaaaagg gttggctggt gcaacccgat tgcctaaccg ttaatgctaa tctggcctaa
       61 ccttttagca tccagaatct gaattttcct cttagtcatg cttattaatc cctccctgat
      121 aaactcgcta agctgacgat taacagtttc tcttgaagtg ctaattaaat cggccagctc
      181 gctgttgttg agcggcattt cgaaggtgct tgagcaaaaa agatcgtcgg acatgcacag
      241 tagaacttcg gcaacccttc ccggaatctg cttttgcgac atgcttgtgc agcgacgata
      301 ttcgacaagt tcgtttcggc aaagatccat aatgagttca taggcaaaat caccattttt
      361 agtgataaaa tctttaaagg tgtcaagcga gatgaagcaa acttcggcat cggaaatggc
      421 tgttgcagag tactgattta tacgatcgcc aaaagtagtc ggaaggccta aatatgtagg
      481 agctttaatg agttttaata ttttatcgcc cgaaggcccc ttcatgtgta cttttaccat
      541 acctcttttc aggtagatag tattggttgc caaggttccc tccctgaata ccacctcacc
      601 ctttttaaac agcacctcgg cgcagccaaa ctccaatgtt tggagttctg tctcgttcag
      661 gtgctttgct gctgacgact tgtaaccaca agttctacat tcgggatatg atataattct
      721 tcagttatct tctgcaaaga tataaaggtt aaagatttat tgtgtgaaaa atctcacagt
      781 taagcgtaag ggatatcaca cctggagtag gtaaaataat gacaagcagc tatacttttg
      841 aattgttaaa tatttaacag attataaccc tataccacat taagacatgg aaatagacgt
      901 tgaaaagcag ctcgaagagc taaagaaaaa agtaggcaag ctcgaaagtg gtcggaagga
      961 caagttgact atggtagtct tttccggcga tatggacaaa cttttggcag caatgatcat
     1021 tgcaaccgga gcaacagcaa tggacatgga ggtaagctta ttcttcacat tctgggctac
     1081 tgctgctctc cgcgatccca agaaaaaagt taaggggaaa aacctcgttt caaaaatgtt
     1141 tggtttcatg ctccccaaag gtgcaaaaaa ggttaagctt tcgaagcttc acatggcagg
     1201 tatgggtaaa accatgctgg agaacattat gaaaaagaag aatgttgcct cccttgatga
     1261 aatgtttaaa acagccgcag aacttggcgt ttctataaac gtttgcgaaa tgtcaatgaa
     1321 tttaatgggt ttcaaacgcg aagagctaat tgactatccc aacctaaaga tttgtggtgt
     1381 aggaacattc ctctcggatg ccgaggagag taaaattcag ctgttcattt agtagtaaac
     1441 ttaaacgatg ccattatgcg cgaaaaattg atcgtaattg ttacagaagg acctgataac
     1501 caggaaaagg caacaattcc ttttgttatg gcaacagcag cacaatcaat ggatgttgat
     1561 gttactatta ttttgcaagc tgagggggca ttacttgtaa aaaaaggtgt ttccgaaaat
     1621 gtaaacgcat cgggctttat gccactaaag caactgcttg atacattcat tgaactaaat
     1681 ggtaagctac ttgtatgcac cccttgcgct aaatcgagag ggattagcga aaatgatcta
     1741 gtcgagggtt ccaaacttat agccgcagga acagttattt cggaatcgtt aagcgcaaac
     1801 gctgtattga gctattaaac acaaatcaaa caacaaaaca atgaacgcag aagaattaaa
     1861 aaatctaaag gtaagccaaa ccgttgatgc acggggtacg gcttgcccag gtcccctttt
     1921 ggctgcaaaa aaagctattg gagaaattga atcgggtgaa atcatggaag tactttcatc
     1981 cgacgaagga acaaagcacg atataccaaa atggtgcgaa aaaatggagc atgagttcct
     2041 gggaattatt gaagaggata gcttcacccg cctatttcta aaaaaggctt agggtgaaaa
     2101 ggataaggaa gcagggcttt ttaagccttg cttcttactt gtacaacaat attttttctg
     2161 cataaaacat agctatcatc atgttaagtg aaacattttc accaaaaatt ctggtcttct
     2221 ccaccgacat aatatcggac ccggggatcg accaagccgg attacgtaag ctgcactacc
     2281 cccctactgt ttacgtaatt agcttgccct gctcatcggg cattaaaccg agatggatta
     2341 tgcgggcgtt cgaaaaaggt ttcgacggtg tttttattgc tgctgatggc catgagtgct
     2401 cctacagccc aaaatgtgct gagcacacca ataatattat tgtagagtcg cagaagttga
     2461 tgaaggagaa caacattaac ccaaagcgaa tacgtatggc tgctttatgt tctgtttgtg
     2521 ctgagccatt tgtgaatcat atgggaaact tctcgaaaat actctcagaa ctgggaagta
     2581 ttggtaccga aaaatacaat ccaagttaaa tctttcgtgc acttcgtgaa atcctttgag
     2641 ccactttgcg gttaaaaatt taaccacaat caacactaag tacacacaac gtttcacaac
     2701 gaaaaattaa agatttattt taaatcttaa tcgatgccag aaaataaaaa aacatttgat
     2761 gcccttgtag tgggaggtgg aatcgcaggt caggaggcag cgctaagttt agccaatatg
     2821 gattacaagg ttcttctagt ggaaaaagag ctttccattg gtggtaagat gattcagctt
     2881 agcaaggtgt ttccaaccct cgactgcgca gcatgtatca ctacaccaaa aatgtcggaa
     2941 acggccaggc atccaaacat tcagttactg ctgaatacag acataatgga gattactaaa
     3001 aataatggat tattcaatgt ca
//
LOCUS       DAOOQX010000185         3019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_39180_length_3019_cov_3.034413, whole genome shotgun sequence.
ACCESSION   DAOOQX010000185 DAOOQX010000000
VERSION     DAOOQX010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39180_length_3019_cov_3.034413"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PLX53_03960"
     CDS             <1..631
                     /locus_tag="PLX53_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72267.1"
                     /translation="VNLVPRDTTLKEIYDIKPPIAEPLTFAEVAPLVGSGLLLAALVV
                     LLVLYVRSRKQNKPFLNLFKPKEPPHLVALRELEQLRNEKLWANENHKLFYTRLVDIL
                     RKYMEGRFGINAPEQTTSEILSELERIEYDFGHLKEELRELLVISDLVKFAKHVPLIQ
                     ENEKNMNFAFDFVNSTRPVETPEIPATTDDTGQQLQDAQSENQTTNKLS"
     gene            642..1625
                     /locus_tag="PLX53_03965"
     CDS             642..1625
                     /locus_tag="PLX53_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOY72268.1"
                     /translation="MVFAAPKMFYLLLLVPVLVAWYIFRQKDSRASLHISTSIGFENV
                     KPGFKVYMRHAPFILRMVAIILIITALARPQSTNIQKNISTEGIDIVLSMDVSGSMLA
                     RDFKPDRLGAAKEIGIKFISGRPNDRIGLVVFAGESFTLCPLTTDHATLINMFHNVNM
                     GILEDGTAIGSGLATAVTRLKDSHAKSRVIILLTDGVNNRGEIAPLTAAEIAKSFGIR
                     VYTVGVGTMGTAPYPVQTPFGTQYQNMKVEIDEDLLTSISKLTGGEYFRATNNTTLEE
                     VYGKIDKLEKSKIEVAEYRSREEKFRGFLLAAFAILVVEFTLRRTLLQGIQ"
     gene            1735..2775
                     /locus_tag="PLX53_03970"
     CDS             1735..2775
                     /locus_tag="PLX53_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOY72269.1"
                     /translation="MFRFAEPKYFYLLLVIPLMVGIYLYSIHRQKKQIAIFGRTDTVM
                     GLLPNLSFTRAWVKFIILLVACVFVVLSLARPQVGSKLTEVKRKGIEIIIALDVSNSM
                     LAEDIQPNRLERAKQSIAQLVDKLTNDRIGLIVFSGDAYVQLPVTSDFASAKMFLSSI
                     SPEMVSKQGTAIGRAIDLASNSFTPQGNTSKALIIISDGENHEDDPVESAKLAAEKGI
                     VIYTIGIGSPSGAPIPIGANQNYLKDENGNIVVSRLDEETLSKIAIEGNGKYIRASNS
                     QFGLLPLFENLSKMSKTEIKEKIYSEYSEQFQYFLAFALALLLIDSLILERKNPKLSR
                     FKLVDTKNGGRQ"
     gene            2772..>3019
                     /locus_tag="PLX53_03975"
     CDS             2772..>3019
                     /locus_tag="PLX53_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72270.1"
                     /translation="MKVFLMSALMLTAVISTAQSERKFIRKGNHDYKKNDFVESEIQY
                     RNALEQNSHSFKGQFNLADALYKQNKFNESLETLNNLN"
BASE COUNT          888 a          635 c          658 g          838 t
ORIGIN      
        1 tgtaaacctg gttcctcgcg atactacact taaggaaatt tatgatatca agccacccat
       61 tgctgaacct ttgacattcg ccgaggttgc cccgctggtt ggttctggtt tactactggc
      121 tgctctagtt gttttgctgg ttttatacgt tagaagtcgt aagcagaata aaccattcct
      181 gaatctattc aaacctaaag aaccccctca cctggttgcg ctcagagaac tcgaacagct
      241 gcgaaacgaa aaactttggg caaatgaaaa tcataaactt ttctacacca ggctagtaga
      301 tattcttcgt aagtacatgg agggtaggtt tggtataaat gctcccgaac aaaccacctc
      361 agaaatttta agcgaacttg aacgaattga gtatgatttt ggccatttaa aggaagaact
      421 gcgtgagtta ctggtaattt ccgatttggt aaagtttgcc aagcatgttc ctctaattca
      481 ggaaaatgaa aagaatatga actttgcatt cgattttgtg aatagcacca gacccgttga
      541 aacgcccgaa attcctgcaa caacggatga cacgggacag caacttcaag atgctcaatc
      601 cgaaaatcaa actactaata aattgagttg atatgtcgga aattgttttt gcagccccca
      661 agatgtttta cctgctcctt ttggtgccgg ttttggttgc atggtacatc tttcgtcaga
      721 aagattccag ggccagcctg catatttcaa cttcaatagg ctttgagaat gtaaagcccg
      781 gctttaaggt ttacatgcgg catgctccct ttattcttag aatggttgca attattctga
      841 tcatcacggc attggcaagg cctcaatcaa ctaacataca aaagaacatc tctactgagg
      901 gtattgatat tgtcctttcc atggatgtct cgggcagcat gctggctcgt gattttaagc
      961 ccgacaggct tggagcggca aaggaaatag gtataaaatt tattagtgga aggcctaatg
     1021 acaggatcgg actggtagtt ttcgccggcg agagctttac cctttgtccg cttactaccg
     1081 accatgccac cctgattaac atgtttcata atgttaacat gggcattctg gaggatggaa
     1141 cagccatagg ctctggtttg gctacagcag ttacgcggtt gaaggatagc catgctaaaa
     1201 gcagggtaat tattttactt accgatgggg ttaacaacag gggcgaaatt gcccctctta
     1261 cagctgccga aatagccaaa agttttggta ttagggttta cacggttgga gtaggcacca
     1321 tgggaactgc accatacccg gtgcaaaccc cttttggtac acagtaccag aacatgaagg
     1381 ttgaaataga tgaggatctg ttaaccagca tctcaaagct aacgggaggt gaatatttcc
     1441 gtgccactaa caacacaacc cttgaggagg tgtatggtaa aattgataag ctcgaaaaat
     1501 ctaaaattga ggtggctgag tatagaagca gggaggaaaa atttcgtgga tttttattgg
     1561 ctgcttttgc catactagtt gttgagttta cattacgcag aactttactg caaggaattc
     1621 agtaaatgct aattgagcca ttcatgagaa gtagttacaa acacataggt aaactgttac
     1681 attgcaaaaa tgtgcctttg ccccttttgt taacaacaaa ttgattgttg agctatgttc
     1741 aggtttgcag aaccaaaata cttttacttg cttctagtga tacctttgat ggtgggtata
     1801 tacctctact ccatacaccg tcaaaaaaaa caaattgcaa tttttggtag gacagacaca
     1861 gtgatgggtt tacttcccaa cctttccttt actagggcat gggtaaaatt tatcattttg
     1921 ctggttgctt gcgtgttcgt tgttctatcg ttagcacgcc cgcaagtagg atcaaagctg
     1981 actgaggtaa agcgtaaggg catcgaaatt attatcgccc tcgatgtttc aaacagcatg
     2041 ctggcggagg atattcaacc taacaggctg gaaagagcaa agcagtcaat tgcccaactt
     2101 gttgacaagc tgactaacga tcgtataggg ttgatagttt tctcaggcga tgcctatgtt
     2161 caactccccg taaccagcga tttcgcctca gctaagatgt ttctttcctc tataagtccc
     2221 gagatggtat caaaacaagg caccgccatt gggcgggcaa ttgatcttgc ctccaactcg
     2281 ttcacccctc agggtaacac cagtaaggcg ctcataatta tttcggatgg cgaaaatcat
     2341 gaggatgacc ctgttgagtc tgccaagctc gctgcagaga aaggtattgt tatatatact
     2401 attggcatag gctcgccttc gggagcgcct atccctattg gcgcaaatca gaactacctt
     2461 aaggacgaaa atggaaacat cgtagtctca agactcgatg aggaaaccct ttccaaaatt
     2521 gccatcgagg gaaatggtaa gtacattaga gcatcgaact cacaatttgg gctgctgcct
     2581 ctatttgaaa acctaagcaa gatgagcaag accgaaatta aagaaaaaat atactcggag
     2641 tacagcgagc aattccaata tttcttagca ttcgccttgg cactgctgct tattgattcc
     2701 ttgattctcg aaaggaaaaa tccaaagcta tcccgtttta agctagttga caccaaaaat
     2761 ggaggcaggc aatgaaagta ttcttgatgt ccgcattaat gctaacagct gtaatttcta
     2821 ctgcccagtc agagcgcaag ttcatacgca agggtaacca cgattataaa aaaaatgatt
     2881 tcgtggaatc cgaaattcag taccgcaacg cgctggaaca aaactctcat tcctttaagg
     2941 gccagtttaa cctggctgat gcgctctata agcagaacaa gtttaacgaa tcgcttgaga
     3001 ccctcaataa tttgaattt
//
LOCUS       DAOOQX010000186         3008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_39384_length_3008_cov_2.500508, whole genome shotgun sequence.
ACCESSION   DAOOQX010000186 DAOOQX010000000
VERSION     DAOOQX010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39384_length_3008_cov_2.500508"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLX53_03980"
     CDS             <1..229
                     /locus_tag="PLX53_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72271.1"
                     /translation="EPYDYRMPVLLKKYLKLNGRILGFNVDPKFNNALDGLLILDLLQ
                     VPIQTITSLSKEINDKSILERFNISDYTFED"
     gene            514..1752
                     /locus_tag="PLX53_03985"
     CDS             514..1752
                     /locus_tag="PLX53_03985"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOY72272.1"
                     /translation="MRLAMYFLPAAIFALSCNRQVVSEPELVERANELHKKIVTIDTH
                     TDAPLTFMRTGYDFSGKNNKAIGSCVDLAKMEAGGLDAAIFAVFISQDECTPDAYRKV
                     NAQALQIASTVINTVAKYPDRAEIAVNPEDIFRLKAAGKRAIYIGMENGYPLGEDLSQ
                     LKVFYDLGARYVTLCHTRNNQICDSSTDPEGSRNNGLSDFGRSVVAEMNRLGMLVDVS
                     HISDKSFYDVLAVSKVPVFASHSCARAICNNPRNLDDNMLKALAKNGGVVQMCILSDY
                     VKQGEKNPVRDSAFRALRRKYGNFRNLTPEQEEQAEADWRNLEQQYPAKLATVSDVVD
                     HIDHIVRVAGIDHVGIGTDFDGGGGVDGCRDASELHAITIELLRRGYSERDIRKIWGE
                     NFLRVFRQARDYAAAHGQGK"
     gene            2114..2746
                     /gene="yihA"
                     /locus_tag="PLX53_03990"
     CDS             2114..2746
                     /gene="yihA"
                     /locus_tag="PLX53_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOY72273.1"
                     /translation="MVIRSAKFVASYPGVDRCPASSIPEYAFIGRSNVGKSSLINMLT
                     GTKTLAKISETPGKTQLINYFLVNESWYLVDLPGYGYAKASKDKRKEFSQIITNYILK
                     RSSLTLLFVLIDSRLEPQPIDIDFINWLGVNQIPLALVFTKTDKTPKALLEANMEAFK
                     NKMMEMWEVLPPIFTSSAVTRTGREDVLSYIDSINANFKSTQPKKVERVW"
     gene            2789..>3008
                     /locus_tag="PLX53_03995"
     CDS             2789..>3008
                     /locus_tag="PLX53_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HOY72274.1"
                     /translation="MHSKHNIKFFTGRSSRYLSERIADSFGIPLGKSNVTVFSDGEFQ
                     PSFDESVRGCTVFIIQSTFPPVDNLFELL"
BASE COUNT          795 a          682 c          680 g          851 t
ORIGIN      
        1 tgaaccctac gactaccgca tgcccgtgct acttaaaaag tacttgaagc tcaacggtag
       61 aatcctaggc tttaacgttg accctaagtt taacaatgcc cttgacggtt tactaatcct
      121 cgaccttctt caggtcccta ttcagaccat tacctccctg tcgaaagaaa ttaacgataa
      181 gtccattctc gagaggttca acattagcga ttatactttt gaggattaaa atgcagcttg
      241 tgtagcctgc tttaccgttt aacctattgg agctagttgt ttgtttgtag aatccccttc
      301 gtgtacgggt acaggggatt tttttcaatt cttgatgttt tctagctaaa ccataaccga
      361 tttgttcggt caatttttct gttcgatagc tggctataaa gtttgaaaac agcttttttg
      421 aaagtttacg cagctgttaa atatggggaa ttgtagtttt tttgtgcatt ttcttttatt
      481 atcctttagt ctttttttaa attttctggt aaaatgagat tagcaatgta ctttctgccg
      541 gcagcaatat ttgcactttc gtgcaacagg caagtagtga gtgaaccgga gttggttgag
      601 cgtgccaatg agctgcataa aaagattgta accattgata cccataccga tgccccactc
      661 accttcatgc gtaccgggta tgatttcagc ggtaaaaaca acaaagccat tggtagctgt
      721 gttgacctag caaagatgga agcaggtggt ctggatgccg caatttttgc tgtatttata
      781 agtcaggatg aatgcacccc cgacgcatac cgaaaagtca acgcacaagc ccttcaaatt
      841 gcgagtaccg ttataaacac cgttgccaag taccccgaca gggctgaaat tgctgtaaac
      901 cccgaggata ttttccggtt gaaagctgcg gggaaacgag ccatttacat tggcatggaa
      961 aacggatacc cgcttgggga ggacttatcg cagcttaagg ttttctatga tttgggcgct
     1021 cgctacgtaa cactctgcca tacccggaac aaccagattt gcgactcctc aaccgaccct
     1081 gaaggttcaa ggaataatgg gcttagcgat ttcgggagat ccgtagtggc cgaaatgaac
     1141 aggctgggta tgctggttga cgtttcgcat atctccgaca agtcgtttta cgacgtgctt
     1201 gccgtttcca aggtgcctgt ttttgcctcg cacagctgtg ccagggcaat ttgcaataac
     1261 cctcgaaacc ttgacgataa catgctgaag gccctagcta aaaatggcgg ggtagttcaa
     1321 atgtgtattc tcagcgacta cgtcaagcag ggtgagaaga atcctgtacg cgatagcgca
     1381 tttagggcgc tgcgtcgcaa gtatggcaac ttccgaaacc ttactcccga acaggaggag
     1441 caggctgagg cagactggcg aaacctcgag cagcagtacc ctgctaagct tgccacggta
     1501 tccgacgtgg ttgaccatat cgaccacatt gttagggttg ccggaattga ccacgtgggc
     1561 atcggaaccg acttcgacgg cggtggaggt gttgatggat gtcgcgatgc ctctgagcta
     1621 cacgcaataa ccattgaact cctaaggcgg gggtattccg agcgggatat caggaaaatc
     1681 tggggcgaga atttcctgcg tgttttccgg caggcccgcg attatgcggc agctcatggg
     1741 cagggcaagt agctattgct gagttgctca caatagtaat acctgcatca ccatagctga
     1801 attttctcca ttcatcctgc aatcaacaca ccagccagct catggctggt ttaaccttta
     1861 tctaacggcg tattatgctg atgtggctac tacccaatga gctatttgca ccggatataa
     1921 agctatccaa gcaagtttcc ccgagcgttc acctttattc tcagcttatt attcccgatg
     1981 aaggatattt tgctgtgttt ttctatttgt atgtattttc tactcttttg accaatggtt
     2041 tgctttttaa ttttataggt ttgtatcttt gaggtggatt ctgggacaat acatcaaact
     2101 aaaatttaca actatggtga ttcgatctgc taaatttgta gcgagctacc ccggtgtcga
     2161 taggtgcccc gcttcgtcaa ttcccgagta tgcatttatt ggccggtcaa acgtaggaaa
     2221 atcgtcgcta atcaacatgc tgacggggac caaaacgctg gctaaaatat ccgaaacccc
     2281 ggggaaaaca cagctcatca actattttct ggtaaacgag tcatggtatc tggttgattt
     2341 gccgggttat ggctatgcca aggcttcaaa agacaaacga aaggagtttt cccaaatcat
     2401 cacaaattac atacttaaac gttcaagcct taccctactt tttgtgctga ttgattcacg
     2461 tcttgaaccc cagccaatag acattgattt tattaactgg ctgggtgtaa accaaatccc
     2521 gctagcgctg gtatttacta aaaccgataa aacgcctaaa gctctccttg aggccaacat
     2581 ggaagctttt aaaaataaaa tgatggagat gtgggaggtg ttacctccaa tatttacctc
     2641 ctcggccgtt acccgaactg gaagggagga tgtgctatcc tacatcgaca gcataaatgc
     2701 aaattttaag tcaacccagc ccaaaaaggt tgaacgggtc tggtaaaatt agtacatttg
     2761 cgggcattaa caggaaaaaa ttcctaaaat gcacagcaag cacaacatta agttctttac
     2821 cggtcgcagc tcccggtatc tctctgaaag aattgccgat agttttggca ttcctcttgg
     2881 gaaatccaac gttacggtat ttagcgatgg cgaatttcag ccttcctttg atgaatcggt
     2941 gaggggctgc accgttttta tcatccaatc aacctttcca cctgtagaca acctattcga
     3001 gttgctgc
//
LOCUS       DAOOQX010000187         3001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_39511_length_3001_cov_2.816701, whole genome shotgun sequence.
ACCESSION   DAOOQX010000187 DAOOQX010000000
VERSION     DAOOQX010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39511_length_3001_cov_2.816701"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLX53_04000"
     CDS             complement(<1..184)
                     /locus_tag="PLX53_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72275.1"
                     /translation="MDKRNGTETSAFGTKGRINHDSSKFYSSKLYSEINNNFIGDKAE
                     NQIPNEYENRIILGSAE"
     gene            complement(187..1047)
                     /locus_tag="PLX53_04005"
     CDS             complement(187..1047)
                     /locus_tag="PLX53_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72276.1"
                     /translation="MILWNNDIEIQFFEEALKNFASPEKLFYKLNTGYFAYIPKGTEN
                     EGHTLQSRNSLIGQFTEKWCKSILEPIAGKFGLYAINGVVCEEIGLSRQSSADLALCT
                     SNKIIQRPENIKLIFEIKMSIVSNYKYTEPNRIEFISDYKHHKGNPSLLRSDSMLKAI
                     GKSISIRVSGNSSCKIPIVTIGNSPISKNYVDKIDFLKSSGVIQGFWSLNPNPCNSEF
                     IKETPQKGFQTFKNQKMITESCKELLESDMLYFSSMVSMPKLGEIIRLANKEKTDAAK
                     AEKFLTLIRG"
     gene            1212..1634
                     /locus_tag="PLX53_04010"
     CDS             1212..1634
                     /locus_tag="PLX53_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOY72277.1"
                     /translation="MEKVAIQAEWLEGLAFETEVNGHKLVVDADENAGGSNRGPRPKP
                     LMLVALAGCTGMDVISILKKMRVDVKRFSVGVEAMQTDEHPRHYVGMNIIYEFWGKDL
                     PPESINKAINLSQEKYCGVSASYRKAMPITFEVKIHEE"
     gene            1648..1857
                     /locus_tag="PLX53_04015"
     CDS             1648..1857
                     /locus_tag="PLX53_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72278.1"
                     /translation="MNITGNTLIEDLVEQLPESVSFLREKGIICIVCGEAVWGTLAEV
                     AQQKGLSDEAIEATIEELNQLKKSK"
     gene            2219..>3001
                     /locus_tag="PLX53_04020"
     CDS             2219..>3001
                     /locus_tag="PLX53_04020"
                     /inference="COORDINATES: protein motif:HMM:NF015548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOY72279.1"
                     /translation="METPAKLYQNVLDARQTEDAIALIHDFFPGKLAAVLNLRRVTAP
                     LIVPSGMGINDDLNGVEQPVRFSAKSLGNREVEVVQSLAKWKRITLARLKVEPGKGIY
                     TNMNALRPDETLDATHSIYVDQWDWEKCIADAQRNLEYFRRTVEQIYSAMKDLESHIF
                     EHFPQLAPVLPDKIHFIHTSELEARFPNLSHRQREYESCRELGAVCIEGIGYPLSNGH
                     PADGRAPDYDDWSTLRGDGKRGLNGDILVWHPTLQNSLELSSM"
BASE COUNT          891 a          659 c          627 g          824 t
ORIGIN      
        1 tttcagctga acccagtatg atccggtttt cgtattcgtt ggggatttga ttttcagcct
       61 tatcacctat aaaattatta tttatctcgg agtatagctt cgagctatag aactttgaac
      121 tgtcatgatt aattctaccc ttagttccaa aggcgctggt ttctgtccca tttcttttat
      181 ccatagctat ccccggatta atgttaaaaa tttttcggct ttcgcagcat ctgtcttctc
      241 tttgttagcc aatctaataa tttcaccaag ttttggcata gaaaccattg atgaaaaata
      301 gagcatatcg ctttctaaca attccttaca actttctgta atcattttct ggtttttgaa
      361 tgtttgaaag cctttttgcg gagtttcttt gataaattcg gaattgcagg gattagggtt
      421 tagcgaccaa aaaccttgaa taacacctga agattttaaa aaatcaattt tatcaacata
      481 atttttgcta attgggctat tgccaatagt aacaatggga attttacacg aggaatttcc
      541 tgaaacccga atgctaatag attttccaat agctttgagc atagaatcgg aacggagcaa
      601 tgaaggatta cccttatgat gcttgtagtc gctaataaat tcaattctgt tcggctcagt
      661 atatttatag tttgaaacga tactcatctt aatttcaaaa ataagcttga tgttttcggg
      721 acgttgaatg attttattcg aagtgcaaag cgccagatcg gcagatgatt gcctcgatag
      781 gccaatttct tcacatacaa cgccattaat ggcgtacagt ccaaatttac cagctatggg
      841 ttcaaggata gatttacacc atttctcggt aaactgccca atcagcgaat tcctactttg
      901 cagggtgtgc ccctcattct ccgtaccttt aggaatatat gcaaaataac ctgtattcaa
      961 cttgtaaaat aacttttccg gagaggcaaa attcttcagt gcttcctcaa aaaattgaat
     1021 ttcaatatcg ttattccaca gaatcattcg caacacattt ttacaaattt agcaattatc
     1081 aagcatatag aagttgctta catgatttct tttagtaccc cgctggtgct ttaccctttc
     1141 atacctgatc tgtaaaaatt tttttgaact ttaccccaaa aattcggttc ttacatatca
     1201 aaactaacac tatggagaaa gtagctattc aggccgaatg gttggaaggc ttggcctttg
     1261 aaaccgaggt aaacggccac aagctggttg ttgatgccga cgaaaatgcc gggggaagca
     1321 accgcggccc ccgccctaaa ccgcttatgc tggttgccct ggccggctgc acgggaatgg
     1381 atgtaatttc tatccttaag aaaatgaggg ttgacgtaaa acggttcagc gttggcgttg
     1441 aggccatgca aaccgatgag caccccaggc actatgttgg gatgaacata atctacgagt
     1501 tctggggaaa ggatcttccg cccgaaagca tcaacaaggc catcaacctg tcgcaggaaa
     1561 agtactgtgg ggttagcgcc tcataccgca aggctatgcc aattactttc gaggtaaaga
     1621 ttcacgaaga gtaacaggag aatacggatg aacataaccg gcaataccct gattgaggac
     1681 ctggtggagc agctacccga atcggtttct ttccttaggg aaaaaggaat tatctgcata
     1741 gtttgcggag aggctgtatg gggaacactt gctgaggtag cccaacaaaa gggactttcc
     1801 gacgaggcta ttgaagcaac tatcgaggag ctaaaccagc taaaaaaatc aaagtaaatt
     1861 ttgagaattt aaaggaaaat cctacattgc ccttgaaaat ttggatgaaa tgattcacat
     1921 attccgcaat aattgcaatt acaatttcaa caataatggc tggttgttga aaggggattg
     1981 ctattgctga aataaacatt gattacccgc aaaaggcttt cccaaaaagg gagagccttt
     2041 tttgattgca gtaacccaac tagaagtaac accatgcaaa ccccgctact aaacggaaat
     2101 aataataacc gcccacctcc ctccgggtca ggatcatgct gtatttgccc ttcccggaaa
     2161 aaccgtgtga agggttttct ttttatgcaa gttcctcaac ctaaaaaaaa taaattacat
     2221 ggaaacacca gctaaattat accaaaacgt tctggatgcc cgccagacag aagatgcaat
     2281 cgccctcatc cacgatttct ttcccgggaa attggcagca gtgctaaacc tacggcgtgt
     2341 taccgcaccg ctgatagtac cctcgggtat gggtataaac gacgacctca acggggttga
     2401 acaacctgtc aggttcagcg ccaaatcgct gggcaacagg gaggttgaag ttgtgcagtc
     2461 gctggccaag tggaaaagga taacccttgc cagacttaaa gtagagccag gaaagggtat
     2521 ctacaccaac atgaatgcac tccgacctga cgaaacgctc gatgccacac attcaattta
     2581 cgttgaccag tgggattggg aaaaatgtat agctgatgct caacgcaacc tggagtactt
     2641 cagaagaacg gttgaacaaa tttactccgc catgaaagat ttggaatctc atatctttga
     2701 gcactttccc caactagcgc cagttttgcc cgataaaata cacttcatcc acaccagcga
     2761 gctggaggca aggtttccaa acctttcgca ccgacaacgc gagtatgaaa gctgcaggga
     2821 acttggagct gtatgtatcg agggaattgg ctatccccta tcgaatggcc atcctgcaga
     2881 tggtcgggca cccgactacg acgactggtc aaccctccga ggtgatggta aaagaggcct
     2941 caacggtgat atcctggttt ggcatcccac ccttcaaaat tcactggagc tctcgtctat
     3001 g
//
LOCUS       DAOOQX010000188         2988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_39759_length_2988_cov_3.058302, whole genome shotgun sequence.
ACCESSION   DAOOQX010000188 DAOOQX010000000
VERSION     DAOOQX010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39759_length_2988_cov_3.058302"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1831
                     /locus_tag="PLX53_04025"
     CDS             <1..1831
                     /locus_tag="PLX53_04025"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOY72280.1"
                     /translation="EELPNPGLSVRFLVRMEDVTGSVLQGVSPNTPEAERSRIVSEHI
                     KSIESAAMAAEPGYLAQVKPFYYGNDYYLLIYEVFNDVRLVGAPPEAIGRFGGDTDNW
                     VWPRHTGDFSLFRIYADKNNKPAPYSPDNVPYKPKKFLKISTKGVNEGDFTMIYGFPG
                     RTQQFITSDAVSLLVNQSNPHKIHLRDIRLSIFNAYMSGNDTIRIQYASKHAGVANAW
                     KKWIGETVGLQRLNAVQWKLEQEQMFTAWANSNPELSREYAGLLPKFKRLYGELAPLS
                     LVADYRNEAVYAVELLGFASRFKDVLAEFSDSTLDTVKVASLKNRLSSYASTFYKNFS
                     SRVDMEVFAAMMYQYHQQIGGDYQPQFLTQVNNQQQDSWMQLAYNIYSKSIFTDSLMV
                     LNLINDMDRSNAQIIASDSIYKIYAQFENIYQSKVDTRYREIQDSIDILYRTYVKGLR
                     EMQPDKMFFPDANSTLRVAYGKVAGFSPRDGVNYLYYTTIDGIAQKSLQSDVPDYKPP
                     VKLMELYRNKDFGRYAVNGTVPVAFIATNHTTGGNSGSPVLDSNGNLVGVNFDRCWES
                     TMSDVMFDPNYCRNIALDIRYVLFIIDKFAGAQHLISEMQLDG"
     gene            2139..2825
                     /locus_tag="PLX53_04030"
     CDS             2139..2825
                     /locus_tag="PLX53_04030"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HOY72281.1"
                     /translation="MANRIMDPIERLMGLRYKSHPWHGVDVGPEVPDVVTAFIEMVTT
                     DTVKYEVDKMSGYLRIDRPQKYSSVLPALYGFIPQTLSDESVAQIARGKTDRKDLSGD
                     GDPLDICVLTEKNIVHGNLLVHAIPIGGFRMIDGNQADDKIIAVLKDDAVYGHIQSID
                     QCPEAVITRLRHYFLTYKDLPGTVRDCEITHVYGVEECHQVINASLDDYRKKFENLDI
                     VLSQYGITKK"
BASE COUNT          840 a          635 c          689 g          824 t
ORIGIN      
        1 cgaagagctg ccaaaccccg gattatccgt ccgtttcctg gttcgaatgg aggatgttac
       61 cggttcggtg ctgcaaggtg tttcgcccaa tacgcctgaa gctgaacgga gcaggattgt
      121 ttctgaacac ataaagtcta tcgagtctgc tgctatggcg gccgaaccag gatacttagc
      181 tcaggtcaag ccattttact atggaaacga ctactacctt ctgatttatg aagtctttaa
      241 cgatgtaagg ttggtaggcg caccacccga agccataggc cgttttggag gcgataccga
      301 taactgggta tggccccgcc atacgggtga tttttccctg tttagaatct acgccgataa
      361 aaacaacaag ccggcaccct actcacccga taatgttcct tataaaccta agaaatttct
      421 gaaaatttca accaagggtg ttaatgaggg tgattttacc atgatttacg gtttcccggg
      481 tagaactcaa cagtttatta catcagatgc tgtaagcctg ctggttaacc agagcaatcc
      541 ccacaaaatt cacctgaggg atatacgtct ttcaattttc aatgcttaca tgtctggcaa
      601 cgataccatc cgaatccaat atgccagcaa acatgcaggg gttgccaatg catggaagaa
      661 atggattggc gaaactgttg ggttgcagcg cctgaatgct gttcagtgga agcttgagca
      721 ggagcaaatg tttaccgcat gggcaaattc aaatcctgaa ctttccaggg agtatgccgg
      781 cttactccct aagtttaaaa ggctctacgg ggagttggca cccctttcgc tcgtagccga
      841 ctacaggaat gaggcggtat atgctgttga gttgctcgga tttgcttcac gttttaaaga
      901 tgttttagct gaattttcgg acagcacgct cgacacggtt aaggtggcat cgcttaaaaa
      961 caggctgtct tcctatgctt ctacatttta caaaaatttt agctcgcggg tagatatgga
     1021 ggtatttgca gccatgatgt accaatacca ccagcaaatc ggtggcgatt accagccaca
     1081 atttttaact caagtcaaca accagcaaca ggatagctgg atgcagctgg cttacaacat
     1141 ttacagtaag tcaattttta ccgacagcct gatggtgctg aacttgataa atgacatgga
     1201 tagaagcaac gctcaaatta ttgcatccga cagcatttac aagatatacg ctcagtttga
     1261 gaacatatac cagtctaagg ttgatactcg ctatcgtgaa attcaggaca gcatagatat
     1321 tctataccga acctacgtta agggactcag ggagatgcag cccgataaaa tgtttttccc
     1381 tgatgccaac tctaccctta gggtggctta tgggaaagtt gccggattct cgcctcgcga
     1441 cggggtaaat tacttgtatt acaccacaat tgacggtatt gcccagaaat cactgcagtc
     1501 ggatgtcccc gactacaaac ctcctgtcaa gctgatggag ctatatcgca ataaggactt
     1561 tggtaggtat gcggtgaatg ggacagttcc tgttgccttt attgcaacaa accatacaac
     1621 aggtggaaac tccggaagtc ctgtgctcga ttcaaatgga aacctcgtgg gggttaactt
     1681 tgaccgatgc tgggaaagta ccatgagcga tgtgatgttc gaccccaact actgccgaaa
     1741 tattgccctg gatattcgct acgtgctgtt tattattgat aaatttgccg gagcacaaca
     1801 cctgattagc gagatgcagc ttgatggcta gctcctaaag ctatcagaaa ttttttgaat
     1861 tacaagttgt tctatcttag agtataatct aatttcatca taggtgaaaa ggcttacacg
     1921 tttaacgggt tcaggctaac cgatgagtgc ctttgcctga attataccaa atcactaaag
     1981 cgtccctgtc cctggacgaa ggaatattaa gatagcttat gggaatttta ctacatgtaa
     2041 ggtttaaagc atttcttttg agaaattgta tgtatatggt gttgaggtgt tggagcgtaa
     2101 aagctgtttt taaaacttta tcctgaaaaa agcattgtat ggcaaataga attatggatc
     2161 ccattgagag gttgatgggt ttaaggtata aatcgcaccc ctggcatggt gttgatgttg
     2221 gtcctgaagt tcctgatgtg gttactgcct ttatcgaaat ggttactacc gataccgtta
     2281 agtacgaggt tgacaagatg tcgggctacc ttaggattga tcgtcctcag aagtactcca
     2341 gcgtgctgcc tgcgctctat gggtttatac cccaaaccct atcggatgaa agtgtagccc
     2401 aaatagcacg cgggaaaacc gaccgcaaag atttatcggg cgatggtgat ccgctggata
     2461 tttgtgttct tacagaaaaa aacatagtcc acggtaacct gctggttcac gcaatcccaa
     2521 ttggtggctt taggatgatt gatggcaatc aggctgatga caaaataatt gcagttctta
     2581 aggacgatgc cgtttatggg catatacaaa gtattgacca gtgtcccgaa gcggttatca
     2641 ccaggttaag gcattacttc cttacctaca aggatttgcc aggcacagta cgcgattgtg
     2701 agataaccca tgtttacgga gtggaggagt gccatcaggt tattaacgca tcactggacg
     2761 actaccgaaa aaaatttgaa aacctcgata ttgttctatc ccagtacggc atcactaaaa
     2821 aataaatacg gaggtgctgt tttacttgcc gattaccctg tgaagcaaaa tttgaattca
     2881 cttaaaacaa tttcctgggg atggggtcat tatcaatgtg aattactcat ccacctattt
     2941 ttcgaaagtg ctaacaagtt aagaaaagcc aggatgccct ggctttac
//
LOCUS       DAOOQX010000189         2985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_39813_length_2985_cov_2.925256, whole genome shotgun sequence.
ACCESSION   DAOOQX010000189 DAOOQX010000000
VERSION     DAOOQX010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39813_length_2985_cov_2.925256"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..794
                     /locus_tag="PLX53_04035"
     CDS             123..794
                     /locus_tag="PLX53_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOY72282.1"
                     /translation="MSIGTNLLETINSLPKGVTLVAVSKTHPPESIMEAYNAGQRVFG
                     ENKVQELVSKYEVLPRDIEWHLIGHLQSNKVKYVVPFVRLIHSVDSLRLLVTIDREAQ
                     KVNRVVDCLLQIHIASEETKFGLSADELEQLLDAEEYNLLQHIRVVGLMGMATFTENK
                     EQVRMEFRFLRQLFERIQEKYFVGKPWFCELSMGMSSDYRIAVEEGSTMVRIGSSIFG
                     QRVYR"
     gene            832..1809
                     /locus_tag="PLX53_04040"
     CDS             832..1809
                     /locus_tag="PLX53_04040"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOY72283.1"
                     /translation="MANLTTSYLGLNLKSPLVVSSSNLTASVERIKNFEEMGAGAVVL
                     KSLFEEQIQHEIGNLAQYSDYTEAADYLKHYVTANNVNTYIELIRKAKSAVGIPVIAS
                     INCMSASQWVTFASQIQEAGADALEVNVFMLPVDPHKTSAEYESIYTSLAEKLTRIIS
                     IPIAFKVGPHFTNPFAVIEKLWYLKIRGVVLFNRFFEPDFDINTLKVIPASIFSHPDD
                     IRNSLRWVGMTNHLVPKIDISASTGVHDGEAMIKMILAGASSVQVCSAIYMNGAKHIA
                     SMLSKLSEWMDTMGFASINDFKGKLNYGQIHDPALYERSQFMKYYSSFE"
     gene            complement(1872..2420)
                     /locus_tag="PLX53_04045"
     CDS             complement(1872..2420)
                     /locus_tag="PLX53_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72284.1"
                     /translation="MISTSEREALVKAFEKRLSEASDEAIIGMLEEIRVSGESYMIPS
                     LVNLLFATRGEALKQAVMNFMVDVKIQAAAEYFVSAIQNNPSSPDLYRLVSVCWQSRL
                     DFSSYIDLFLTLLCNADLQTSIEAFSVIENSLDAKSDEEVKELTSRARSMVEHAAEPN
                     KPLVAQLVKLLENFQRDEWQQL"
     gene            complement(2756..>2985)
                     /locus_tag="PLX53_04050"
     CDS             complement(2756..>2985)
                     /locus_tag="PLX53_04050"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOY72285.1"
                     /translation="EAVRCKLEHVGIVVVISPDQIVVVGNDYNDSDMLQLTAHSFVVE
                     NAPELLKKSYTVVPSNDSSGFSYAINKVLGM"
BASE COUNT          820 a          655 c          692 g          818 t
ORIGIN      
        1 gagtttgaga aaaaaaatcg acttgcaagg agttaaggtt aggacttaag agcgatgagc
       61 ggcaaaattt ttccttttgc ccataggcaa aatattctat atttgcatca aaccttactg
      121 taatgagtat tgggacaaat cttcttgaaa ctataaactc gttgccaaaa ggggttacgc
      181 tggttgccgt ttcaaaaacc catcctcccg aaagcatcat ggaggcatat aatgcaggcc
      241 aaagggtttt cggggagaat aaggtgcagg agctggtatc gaagtacgaa gtgttgccaa
      301 gagatattga gtggcatcta ataggccatc ttcaatcgaa taaggtaaag tacgtggttc
      361 cctttgtacg gctaatccac tcggtggata gtttaaggct actggttacc attgatagag
      421 aagctcaaaa ggttaaccga gttgtggatt gtttgttgca aatccacata gcctcggagg
      481 aaactaagtt tgggctgtcg gccgatgagc tggaacagct cctggatgct gaggagtata
      541 atctgctgca gcatattcgg gttgttggat tgatgggaat ggctactttt actgaaaaca
      601 aggaacaggt gagaatggaa ttcaggttcc tcaggcaact tttcgaaaga atacaggaaa
      661 aatactttgt cgggaaacct tggttttgtg aactctccat gggcatgtcc tccgactaca
      721 ggattgctgt tgaggaggga agcaccatgg tgagaattgg cagcagcatt tttggccagc
      781 gagtttacag gtaagttcat cgtaaccata aatcaactaa aaaatacagc tatggcaaac
      841 cttacaacct catacctagg gttaaaccta aaatcgccgc tggtggtgag cagctccaac
      901 cttaccgcct cggttgagcg gattaaaaat tttgaggaga tgggtgcagg cgccgttgtg
      961 ctgaagtcgc tgttcgaaga gcaaattcaa cacgagattg gcaacctggc gcagtactcc
     1021 gactataccg aagcagccga ctacctgaag cattacgtta ctgccaacaa cgtgaacacc
     1081 tacattgagc ttatacgcaa ggcaaaatca gctgtcggta ttccggttat tgccagcatc
     1141 aactgcatga gcgcttcgca gtgggttact tttgcctcgc agattcagga ggcgggtgcc
     1201 gatgcgctgg aggtaaacgt gttcatgcta ccagttgacc cccataaaac ctctgctgaa
     1261 tacgagtcaa tctacacaag cctggccgag aagcttaccc ggataatttc catacccata
     1321 gcttttaagg tgggaccaca ttttaccaac ccttttgcgg tgattgaaaa gctttggtac
     1381 ctcaagatta ggggagtggt gctttttaac cggtttttcg aacccgattt cgacataaac
     1441 accctaaagg ttatacccgc atctattttc agccatcccg acgatattcg gaactcgctg
     1501 cggtgggtgg gaatgacaaa ccacctggtg ccaaagattg acatctcggc ttctacggga
     1561 gttcacgacg gtgaggcaat gataaaaatg attctggctg gcgcctcgtc ggttcaggtt
     1621 tgctcggcta tctacatgaa tggagcaaag catattgcct caatgcttag taagctgtcc
     1681 gaatggatgg atactatggg atttgccagc atcaacgatt ttaaagggaa attgaactac
     1741 ggtcaaattc atgatcctgc actctacgag cgtagccagt tcatgaagta ctactccagc
     1801 ttcgagtagg ccttccctta aaacggaatg ttgtattagg taagaaagtt tgttagagtg
     1861 atatagtcaa cctataattg ctgccactca tcacgctgaa aattttccaa cagcttaacc
     1921 agctgggcaa ccaatggttt attgggttct gctgcgtgtt caaccattga ccttgctctg
     1981 ctggttagct cctttacctc ttcatccgac ttggcatcaa gtgagttttc gattacagag
     2041 aaggcttcga tgctggtctg cagatcagcg ttacacagta aggtaagaaa taggtcaata
     2101 taagagctga aatcaagccg gctttgccag cacaccgaga ccagcctgta caggtcgggt
     2161 gaagaggggt tattttgaat ggcgctcaca aagtactcgg cagcagcctg aattttgaca
     2221 tcaaccatga aattcattac agcctgttta agcgcttcac ccctggtggc aaaaagcagg
     2281 ttgaccagag agggaatcat gtagctctca ccagatactc ttatttcttc gagcatgcca
     2341 attatagcct cgtccgaagc ctcagatagc cttttttcaa aagcctttac tagtgcttct
     2401 ctctcactgg tgctaatcat agctgttctt ttaattgcaa aattaacaat gttttacctt
     2461 ttttatcctt agattttcat gactttttta aaaccccgaa ggagcaaaac atactaaccc
     2521 taggcaacgc ccggggttta ataaaaaccg aaaatatctg cgatgcaagc agtatctccc
     2581 caagagccgt ttagttcctt tgcatcgtaa taggaaaaaa tttccttcga ggcatgagta
     2641 gatcagaggt atttttcttc cttttaccct tcaactaatt cagggcatgc ttttagcttc
     2701 ttcccggttt acgtcgtgcc tctcgatgtt tttgtgatat tgaaatggtt cgtagttaca
     2761 tgcccaacac cttgttaatg gcatagctaa accctgaact atcgtttgat ggaacaaccg
     2821 tataggattt ttttagcagt tcaggggcgt tttccacaac gaagctgtgc gctgtaagct
     2881 ggagcatgtc ggaatcgttg tagtcatttc ccaccacaac aatttgatcg ggagaaatga
     2941 ctacaacgat tccgacatgc tccagcttac agcgcacagc ttcgt
//
LOCUS       DAOOQX010000190         2980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_39885_length_2980_cov_2.745299, whole genome shotgun sequence.
ACCESSION   DAOOQX010000190 DAOOQX010000000
VERSION     DAOOQX010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39885_length_2980_cov_2.745299"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLX53_04055"
     CDS             complement(<1..188)
                     /locus_tag="PLX53_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOY72286.1"
                     /translation="MEKLVKISAISYLNTAPFAYGLRHSDIFSKIQLLSDYPSECARK
                     LQYNEVDISLLPVGALPSL"
     gene            300..1646
                     /gene="purB"
                     /locus_tag="PLX53_04060"
     CDS             300..1646
                     /gene="purB"
                     /locus_tag="PLX53_04060"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOY72287.1"
                     /translation="MSLNALHAISPIDGRYYSKTKELQNYFSEFALIKYRVWVEVEYF
                     IALCNLPLPQLKKFRTGNFDKLRGIYEKFAEGDAARIKNIESTTNHDVKAVEYFLREK
                     FDELGFSDYKEFIHFGLTSQDINNTAIPASIRDFMNSCYYPTLSKLLEKLHDLSNEWA
                     EIPMLAHTHGQPASPTRLGKEIRVFAERIEVQLLQLRSIPYSAKFGGATGNLNAHLVA
                     YPEVDWVAFANGFVENTLGLKRSQTTTQIEHYDNLAALFDCLRRINTILIDLCRDFWA
                     YISMEYFKQRIVEGEVGSSAMPHKVNPIDFENAEGNLGLANAILDHMATKLPISRLQR
                     DLSDSTVLRNVGVPFAHIQIAIQSIVKGLNKLIINNEVIKNDLETNWMVVAEAIQTIL
                     RREGYPNPYEALKKLTRNNKNVTQKDFLTFIDELDIDGRVKTELKSITPFNYLGVK"
     gene            1711..>2980
                     /locus_tag="PLX53_04065"
     CDS             1711..>2980
                     /locus_tag="PLX53_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY72288.1"
                     /translation="MKKFFSLLKFITPYKWHVVANVVGNILGAIFSLFSLAMLIPFLQ
                     LLFGTVPLVEVKPEFTMTIKSLNDTLNYMLSEMIRNHDRSTAMLFVIGLVLTASFLKN
                     FFTYMSLYYLAPIRTGVLRDIRNTLYRKVVDLPLGYFTEEKKGDVISKMTNDVNEIEV
                     SIIRSLEMIFRDPIIIIIHLIGLIYISPQLTLFVLLLLPLTGVVIGRVGKTLKKTSFK
                     GQRKMGAILTLIEETLSGLRVVKAFNAEKKVISRFESVNNFYTLLMKKMWRRRDLASP
                     LSEFLGTIVVVLVLWFGGNLIFKGKGTLTPEALIAYIGIFYMIINPAKSFSNAYYNVL
                     KGMASADRIDTILSAENPIKIKPEAKVLTEFSSAIEFRNVNFRYQSEPVLHNINLVVE
                     KGKTVALVGQSGSGKSTLVDLLPRFWDVTEG"
BASE COUNT          857 a          675 c          654 g          794 t
ORIGIN      
        1 agagatggta gagcccctac aggcaatagg ctgatgtcga cctcgttgta ttgtaactta
       61 cgggcacatt ctgaggggta atcggaaaga agttgtattt tagagaatat gtccgaatgc
      121 cttaacccgt atgcaaatgg tgcggtatta aggtaggaaa tggccgaaat tttaaccagt
      181 ttttccataa tcagaagttc cgaaagttag taaaatattg gtagctgaaa tataacttgt
      241 aggaaaccct acataatagc taatgtttag taattttgta aaaattcaaa actatactta
      301 tgtcgctcaa tgccctacac gccatatcgc ctatagatgg aagatactac agcaaaacca
      361 aggaactaca gaactatttt tcggagttcg cccttatcaa gtacagggtg tgggttgaag
      421 tggagtattt tattgccctt tgcaacttgc cgcttccaca actcaaaaag tttcgaaccg
      481 gaaatttcga taagcttaga ggtatatatg aaaaatttgc tgagggtgat gcagccagaa
      541 taaagaatat tgagagcaca accaaccacg atgtcaaagc ggttgagtac tttttacggg
      601 aaaaattcga tgagctgggg ttttcagact ataaggagtt tattcacttt gggctaacct
      661 cgcaggatat aaacaacacg gccattccgg catcaattcg tgatttcatg aatagctgct
      721 actaccccac cctctcgaaa ctcctggaga aacttcacga cctgagcaac gaatgggctg
      781 aaattcctat gctagcgcac acacacggtc agcctgcttc acccaccagg ttgggaaagg
      841 aaattcgggt cttcgctgag cggatagagg ttcagctatt gcagctcagg tcaattccat
      901 attccgccaa gtttggcggg gctaccggta acttaaacgc tcatttggta gcctaccccg
      961 aggtggattg ggtggcattt gccaatggct ttgtcgagaa tactttgggg ttaaagcgct
     1021 ctcaaaccac cacgcagatt gagcattacg ataaccttgc agcacttttt gactgccttc
     1081 gccgaattaa caccatcctg attgacctgt gccgtgactt ctgggcttat atttcaatgg
     1141 agtacttcaa gcaaagaatt gttgagggtg aagtgggctc atcggcaatg ccccataagg
     1201 ttaaccccat tgattttgaa aatgccgaag gcaatttagg cttggcaaat gccatactcg
     1261 atcacatggc aaccaagctg cccatttcga gacttcagcg cgacctttcc gattccaccg
     1321 ttctgcgcaa cgtaggggtt ccatttgccc acattcagat tgccattcaa tccattgtca
     1381 aagggctaaa caagctgatt atcaacaacg aagtcatcaa aaatgatctg gaaaccaact
     1441 ggatggttgt tgccgaagcc attcagacta ttctccggcg cgaaggatat ccaaacccct
     1501 atgaggctct taaaaagctc acccgcaaca acaaaaacgt tacgcaaaag gatttcctca
     1561 ccttcattga tgagctggat atagatggta gggttaaaac cgagctgaaa agtattaccc
     1621 cgttcaacta cctcggagtg aagtaggtat tcttctaaat tttctgaaat attaaactta
     1681 cctttacacg ctttttcaca aaaatctcaa atgaagaaat ttttcagcct actcaaattt
     1741 atcaccccat ataaatggca tgttgtagct aacgttgtag gtaatatttt aggtgccata
     1801 ttctcgctct tctcattggc catgctcatc ccgttcctgc agctgctctt tggaactgtt
     1861 ccgctggttg aggtgaaacc ggagtttacc atgaccatta aatccctgaa cgacacccta
     1921 aactacatgc ttagcgagat gattcgcaat cacgaccggt caacagccat gctattcgtc
     1981 ataggactgg tgcttacggc ctcgttccta aaaaactttt tcacctacat gtcgctttac
     2041 tacctggcac ccattcgcac aggtgtgctc agggacatac gaaacacact ttacaggaag
     2101 gtagttgacc tgccattggg atacttcacc gaggagaaaa agggtgatgt catcagcaaa
     2161 atgaccaacg atgtgaacga aattgaggtt tccatcattc gttcgctcga aatgatattc
     2221 agagatccta tcatcatcat cattcacctt atagggttaa tctacataag cccacagcta
     2281 acgctttttg tgctgctgct actaccccta accggggttg taattggccg ggttggcaaa
     2341 accctaaaaa agacttcgtt taaggggcaa cgtaaaatgg gggccatact taccctgatt
     2401 gaggaaacgc taagtggact aagggttgta aaagccttta atgctgaaaa aaaagttatc
     2461 tcccgttttg aaagcgtcaa taacttctac accctgttga tgaaaaaaat gtggcgcaga
     2521 agggatctgg cttcacccct aagcgagttc ttgggaacaa tcgtagttgt gttggtgcta
     2581 tggtttgggg gaaatctgat ttttaaggga aaagggacgc taacccccga ggcgctaata
     2641 gcctacattg gcatattcta catgattatc aaccccgcca agtcgttctc caatgcttac
     2701 tacaacgtgc tgaagggaat ggcctctgcc gaccgcattg atactattct ttcggccgag
     2761 aatccaatta agataaaacc cgaagctaaa gtgctgacag agttctcctc ggccatagag
     2821 tttcgtaatg tcaacttcag gtaccaaagt gaacctgtgc tccataatat caaccttgta
     2881 gttgaaaagg ggaaaaccgt ggctctggta gggcagtcag gttctggcaa aagcaccctg
     2941 gtggacctgc tgccacgttt ctgggatgta accgaaggtg
//
LOCUS       DAOOQX010000191         2978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_39923_length_2978_cov_3.187137, whole genome shotgun sequence.
ACCESSION   DAOOQX010000191 DAOOQX010000000
VERSION     DAOOQX010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39923_length_2978_cov_3.187137"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..2008)
                     /locus_tag="PLX53_04070"
     CDS             complement(74..2008)
                     /locus_tag="PLX53_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HOY72289.1"
                     /translation="MQRRQRVTAIITATIGLILVLYGLFRNRMLSYVFEQKTERLTTA
                     YNLQVAADRLKFRGIRTLDISNLVVLNSHADTLAQVGAARIKLGLLHLLVLKVNPLAI
                     ELKDSWFNLNTLLERNSIQNKEAITAEANKQEKFNLLPIYKVIKGTFGLSTAKINIDN
                     LTISYIDSLHYETATVTSWNYKNSRFSASVATQHGDRRGSFELAGESSKSRNSFRVWV
                     YGRSFQVEIPFIEALTGVGVYLDSAKLDFRAGELHGNLMALNIETMAKNISMKGSRIA
                     PYPMNIDSASLKLDIKLKPSEICLDSTSTLSLNRLSTNFKSCYTLDKSPTLTLKFKTA
                     TTRWQDLVESLPSGLFTHIAGMKLRGTFSYTLKARVNPESPDSLVIEPLLSSSGFGIS
                     SFGQTNFYALNDTFTHRVYKDGRWIRNIRVSSDNPEFTPLNHISPFLKWAIITSEDGN
                     FYTHGGFDLDGIRYALACNIRERRFARGGSTITQQLIKNLYLNQEKNIARKAEEMLMV
                     WIIENTAAVSKDRMLEIYLNIIEWGPDVYGIAEAAGYYFNKKPNELTLQESLFLAYIV
                     PRPTKFKYLFDDQDRLKPFLYQNFTDVASKMLMRGYITENDFQAIDSTALQLPLHGRA
                     LELIAVEPDLADSTQLVEVE"
     gene            complement(2095..>2978)
                     /locus_tag="PLX53_04075"
     CDS             complement(2095..>2978)
                     /locus_tag="PLX53_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72290.1"
                     /translation="LRIRHAMFKLKWATTELLVGQYWHPVFVTDVAPSVISFGSGAPF
                     HSLNRSPQIRFSYFPYPMVRISLTALMQSYHKSSGPEDAQRNSGKPELMLQAAAGSAE
                     EFLAGLSVGYKWLTPRLVTQTGYATCKTSGQYLFSAFLLKKLGKTVVKAEAVYGQNPT
                     HLNMIGGYGMASSSGPAPNDDYDYTSLNTLSTWVDVQHQAGKMGVGLFAGYSKLYGAD
                     KNYTSLAGYSRNDDLNNIYRISPRLSYQAESITFALEYMLTAATYGSTFDNRHRVTSS
                     LDPVYNHRATLNIKYSF"
BASE COUNT          711 a          682 c          750 g          835 t
ORIGIN      
        1 actgtttgca tggagaatta catccggtgc acctagaatg tattctggct aaatgatagc
       61 ccacatattc cccttattcc acctctacta gctgggttga gtcggccaaa tcgggctcta
      121 ctgcgattag ctctaaagcc cttccatgca gcggcagctg cagcgctgtg gagtcaatgg
      181 cctgaaaatc attttcggtg atgtaacccc tcatgagcat tttcgatgca acatcggtaa
      241 aattctggta caggaacggt ttcagcctat cctgatcgtc gaacaggtac ttgaattttg
      301 ttggtcgggg aacaatatag gccaggaata gcgactcctg aagtgtaagc tcgttgggct
      361 ttttattgaa gtagtagccg gctgcctcgg caattccgta aacatcagga ccccactcaa
      421 tgatgttcag gtaaatttcc aacatgcggt ccttgctcac cgccgcagta ttttctatta
      481 tccacaccat tagcatttcc tctgccttac gggcaatgtt tttttcctgg ttcaggtata
      541 ggtttttgat aagctgctgg gtaatggtgc tcccaccacg tgcaaatctc ctttcacgta
      601 tattacaggc caacgcatag cgtatgccat cgaggtcaaa tccgccgtgg gtataaaagt
      661 tgccatcttc actggtgatt atagcccatt tcaagaatgg cgaaatatgg tttagcggag
      721 taaattcagg attatcggag ctaacccgaa tgttgcgaat ccatctgcca tccttgtaaa
      781 ccctatgggt aaaggtatcg ttaagggcgt aaaagttggt ttgtccaaat gagctgattc
      841 caaacccgct ggagcttagc agcggttcaa taaccaggct atctggactt tcagggttta
      901 ccctggcttt aagagtgtag ctgaatgttc ccctaagttt catcccggca atatgggtga
      961 ataaacctga gggaagcgac tccaccagat cctgccagcg ggtagtagcg gttttaaact
     1021 tcaacgtaag ggttggagat ttgtccaggg tgtaacacga cttgaagttg gttgaaagtc
     1081 ggtttaagct taacgttgag gttgaatcga ggcagatttc gctaggcttg agctttatgt
     1141 ccagcttaag cgaagcgctg tctatattca tcgggtatgg ggctattctg cttcccttca
     1201 tcgagatgtt tttggccatg gtctcaatgt ttaatgccat aaggttacca tgcagctcgc
     1261 ctgcacgaaa gtcaagtttc gcgctatcca ggtacactcc tacgcctgtg agcgcttcga
     1321 taaatgggat ttccacctga aacgagcgac cataaaccca taccctgaag ctgttacggc
     1381 tcttgctcga ttcgccggcc agctcaaagc taccccttct atcgccatgc tgggtagcta
     1441 ccgatgcgga gaaacggctg tttttgtaat tccaactggt tactgtagcc gtttcgtagt
     1501 gcaacgagtc gatgtacgaa atggtcaaat tatcaatgtt gattttagct gttgataggc
     1561 caaatgtgcc cttgataact ttataaatgg gtagcaggtt aaatttctcc tgtttgttgg
     1621 cttccgctgt aatggcttct ttattttgaa tggaatttcg ttcaagcaac gtatttaggt
     1681 taaaccatga atcctttagc tctatggcca gcgggttaac cttcaataca agcaggtgaa
     1741 gtaaacccag cttgatgcga gcagctccaa cctgggcaag ggtatccgca tggctgttga
     1801 gcacaaccag gttgctaata tccagcgttc tgattcccct gaattttagc ctgtctgctg
     1861 ccacttgtag gttgtatgcc gttgttagcc tctcagtctt ttgctcaaaa acgtagctta
     1921 acatcctgtt tctgaacagt ccgtataaaa ccagtatcag ccctatggtt gctgtaatga
     1981 tggctgtaac cctttgtctg cgctgcataa ggtttaattt tatgatgtag ctctccggag
     2041 ctagctgctt ggttttgggt agctgtccgc agcattatgc cgctggtcgg gaggctagaa
     2101 tgaatacttt atgttaaggg ttgcacggtg gttgtaaacg ggatcgagcg atgaggtaac
     2161 cctgtgcctg ttgtcaaatg tactaccata agtagcggct gtcagcatgt actccagggc
     2221 aaaggtaata ctttccgcct ggtatgatag acggggtgaa atcctgtaga tgttgttcag
     2281 gtcgtcgtta cgggagtaac ccgcaaggct tgtgtagttt ttgtcggcgc cgtaaagttt
     2341 agagtacccg gcaaacaggc caactcccat tttccccgcc tggtgctgaa catcaaccca
     2401 tgtgctaagg gtgttaaggc tggtgtagtc gtaatcatcg ttgggagctg gaccggaact
     2461 ggaagccatt ccgtaaccgc caatcatatt caggtgggtt gggttctgcc cgtaaactgc
     2521 ttctgcctta acaacagttt taccaagttt tttcaataaa aatgcgctaa aaaggtactg
     2581 gccggatgtt ttacacgtgg catagcctgt ttgggttaca agcctggggg tgagccactt
     2641 atatccgacc gacaacccgg ccaaaaattc ttcagcacta cctgcagcgg cttgcagcat
     2701 tagctcgggt ttgccggagt tacgctgggc atcctcgggg ccggatgact tgtggtagct
     2761 ttgcatgagg gcggtaagcg agattctgac catagggtaa gggaagtagc tgaagcgaat
     2821 ctggggcgaa cggttgagcg agtggaacgg cgctcccgag ccaaacgaaa ttactgatgg
     2881 tgcaacatcg gtaacaaaaa ccgggtgcca gtactggcca accagcagct cggtggtagc
     2941 ccactttagc ttgaacattg catgcctgat gcgcaaca
//
LOCUS       DAOOQX010000192         2965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_40176_length_2965_cov_2.769416, whole genome shotgun sequence.
ACCESSION   DAOOQX010000192 DAOOQX010000000
VERSION     DAOOQX010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40176_length_2965_cov_2.769416"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PLX53_04080"
     CDS             <1..484
                     /locus_tag="PLX53_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HOY72291.1"
                     /translation="EISGDAGVDVLSFGGTKNGLMYGEAVVFFNSSIAKDFMYTRKQD
                     LQLASKMRFISAQFIAYLSNNQWATTARHSNQMAQLLADKLRRFSAVKISQKVQVNEV
                     LAIIPPEIIPELQKEYFFYIWDEATSEVRLMTSWDTTEEDIDRFTEILEKLLKKFNML
                     "
     gene            659..1990
                     /locus_tag="PLX53_04085"
     CDS             659..1990
                     /locus_tag="PLX53_04085"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOY72292.1"
                     /translation="MHKRVKFIAAAVFILLGAIFLAFRYDKGVFSKGLTYSGLKYPTV
                     NTLKITNALTSSSEYEWLNNAFNSFIAKWELTGASVAIAKDGRLIYARGFGYASKEEN
                     IPMEPYNLLRVASVSKLITATAVMKLVEENKIQLDSKVFGPNGILNDNIFLNYKDKRV
                     EDITVKNLLNHSAGWSTRWGDHMFINEVVARELHKELPVSLEDIVVFALSKKLHFAPG
                     SHSSYSNLGYAILELVIEKITHTDYETFVQRSIFRPLSIYDARIAQNWDSLRYPNEAR
                     YYEVNEAERVMAFDGSKEGVLKCRGGDDVHTLGAAGGWVISPISLTRFLLAIDGSSSF
                     PDILSKESVAVMTQTSNDKFHPIGWRWVMPGGKLWRSGSLPGTTALAVSSSNGYTYVF
                     ISNTSPWKGARFPYIVDRFFDKLLPGIEGKLPGRNLFFPETSTMGGVASLN"
     gene            complement(1977..2579)
                     /locus_tag="PLX53_04090"
     CDS             complement(1977..2579)
                     /locus_tag="PLX53_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOY72293.1"
                     /translation="MPISTKQIPIFPLEVFLLPGEELPIRIFEPRYQELVNDLDKTGE
                     SFGIPFVLNEKIMAYGSEVELSNIVARNSLGQMVVIVKGVRNFHLLQYSEAPTSKPYG
                     SGAIEYIDDDFRSTNPELIVLAKKLKLEISENLGILVKDEAISLASVAKGLMMTSEDK
                     YTFYSLRSKTKMENFLIKRLRFIEQIQKQEKLLANNFSLN"
     gene            complement(2646..>2965)
                     /gene="msrB"
                     /locus_tag="PLX53_04095"
     CDS             complement(2646..>2965)
                     /gene="msrB"
                     /locus_tag="PLX53_04095"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOY72294.1"
                     /translation="TPFTGEYWNHFEKGVYVCVCCGAELFESETKFHSSCGWPSFFGS
                     KFEENISFNEDSSHGMVRTEVVCKKCGAHLGHIFNDGPKPIGLRYCINSASLKFIPDK
                     VEQ"
BASE COUNT          772 a          634 c          671 g          888 t
ORIGIN      
        1 agagatttca ggggatgccg gtgttgatgt gctttcgttt ggcggtacaa aaaatggact
       61 gatgtatggc gaggcagttg tgtttttcaa ctcctccatt gctaaggatt ttatgtacac
      121 ccgaaagcag gatttacagc ttgcttctaa aatgcggttc atttccgccc agttcatagc
      181 ctacctgagc aataaccaat gggctactac tgccaggcat tccaaccaaa tggcccagct
      241 gttggctgat aagctccgga ggtttagcgc cgttaagatc tcgcaaaaag tgcaggtaaa
      301 cgaagtgctt gccatcattc ctcctgaaat tattccagaa ctgcaaaagg agtacttctt
      361 ctacatttgg gatgaagcta cttcggaagt tcgcctgatg acctcatggg ataccaccga
      421 ggaggatatt gataggttta ccgaaattct tgagaagctt ctaaagaaat tcaacatgct
      481 ctagttcggc actacaaata ggttagaacc agggaattgc ctttttcaag gttttgccat
      541 ttgcgaatgg ttaattttcc atgcttattt atctggtctt cgtgagtggg tgtaaatttt
      601 tttgtcattt actctaatca tagctacttt tgccgctggg taacttcatt gaaagctaat
      661 gcacaaaagg gtaaaattta ttgctgcagc tgtattcatt cttttgggag caattttcct
      721 ggcgtttcgt tatgataagg gcgtattctc taagggtctt acctattctg gtttaaaata
      781 cccaacggtt aataccctta aaattaccaa tgctttaacc agtagcagcg agtatgaatg
      841 gctgaacaac gctttcaaca gcttcattgc caaatgggag ttaaccggtg ccagcgtggc
      901 tatagccaag gatggacggc taatttatgc acggggcttt ggatacgcct caaaggagga
      961 gaatatccca atggagccat acaaccttct aagagtggca agtgtttcaa agctaattac
     1021 ggctactgct gtgatgaagc ttgttgagga gaataagatt cagctcgatt ccaaagtttt
     1081 tggacccaat ggaattctta acgacaacat ttttttgaac tacaaggata aaagggtaga
     1141 ggacattacc gttaaaaatc tattaaatca ttctgcggga tggtcaaccc ggtggggcga
     1201 tcacatgttt atcaacgagg tggttgcccg tgagctgcac aaggagttgc ccgtttcatt
     1261 ggaggatatc gtagtatttg ccctttccaa aaaattacat tttgcacccg gttcccacag
     1321 ctcgtatagc aacctgggct acgcaattct ggagcttgtt attgaaaaaa ttacccatac
     1381 ggattacgag acttttgtac agcgtagcat tttccgtccc ttaagtattt atgatgcccg
     1441 cattgcccag aactgggaca gcttacgcta tccaaacgaa gctcgctact acgaggttaa
     1501 cgaggcagaa cgcgttatgg cttttgatgg ttcaaaggaa ggagttctga aatgtagggg
     1561 tggcgatgac gttcatactc ttggggccgc cggtggatgg gttatttcac ccatcagcct
     1621 taccagattt ctcctggcta ttgatgggag cagcagcttc cctgatattc tatcgaaaga
     1681 gtcagttgcc gttatgaccc aaacaagcaa cgataagttt catcctattg gatggaggtg
     1741 ggttatgccc ggcggaaaac tttggcgttc gggttcttta cccggaacaa ctgcactggc
     1801 agtttcgagt agcaacggtt acacctatgt ttttatttcg aataccagcc cgtggaaggg
     1861 agctagattt ccctatattg ttgacaggtt ttttgacaag ctgttgccgg gcatcgaggg
     1921 aaaacttccg ggtagaaatc tcttttttcc cgaaaccagc acaatgggcg gtgtggctag
     1981 tttaaactga aattatttgc caacagcttt tcctgctttt gaatttgctc aataaacctc
     2041 aaccttttaa tcaaaaagtt ttccattttt gtttttgagc gaagcgagta aaaggtgtat
     2101 ttatcttctg aggtcatcat cagccctttt gcaacgctcg ccaggctaat tgcctcatct
     2161 ttaactagga ttccaaggtt ttcactaatt tcaagtttca gcttctttgc cagcacgatt
     2221 agctccgggt tggtggagcg gaaatcatca tcaatatact caattgcccc gctgccgtat
     2281 ggtttacttg taggcgcctc gctatactga agtaagtgga agtttctaac ccccttaact
     2341 attacgacca tttgccctag gctgtttcgg gctacaatgt ttgagagttc gacctcgctt
     2401 ccgtaggcca ttatcttttc gtttaaaacg aagggtattc caaaactctc accggtcttg
     2461 tccaggtcat ttaccagctc ctggtagcgg ggttcaaaaa ttctgattgg gagttcctct
     2521 ccaggtagca ggaaaacttc cagggggaaa attggtattt gctttgttga aattggcata
     2581 tggttatttg tttagctaag gttttgattc ctggtagcct aattatcggc tatactatga
     2641 aataattatt gttcaacttt atcgggaata aatttaagcg atgcggagtt aatgcagtac
     2701 cgcaagccga tgggctttgg gccatcgttg aatatgtgtc ctaaatgggc accacacttc
     2761 ttacatacca cctcagtcct aaccatgcca tggctggagt cctcgttgaa gcttatgttt
     2821 tcttcaaact tactgccaaa aaagctgggc cacccacagc tggagtgaaa cttggtttcc
     2881 gactcaaaaa gttcggcacc gcagcaaacg caaacatata ctcccttttc aaaatgattc
     2941 cagtactcac ctgtaaaagg ggttt
//
LOCUS       DAOOQX010000193         2962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_40230_length_2962_cov_2.719298, whole genome shotgun sequence.
ACCESSION   DAOOQX010000193 DAOOQX010000000
VERSION     DAOOQX010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40230_length_2962_cov_2.719298"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(410..485)
                     /locus_tag="PLX53_04100"
     tRNA            complement(410..485)
                     /locus_tag="PLX53_04100"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(448..450),aa:His,seq:gtg)
     gene            621..1922
                     /locus_tag="PLX53_04105"
     CDS             621..1922
                     /locus_tag="PLX53_04105"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOY72295.1"
                     /translation="MKLDNTRALQSYQIIRFLAVFTIGMAMVKLSLPQAQVGQYEVVL
                     FIASLLTTFWITGIIQALLPMTSSSVSRDSGSKRPEIFCALVVVVALTLVSALLLLVF
                     KNLIGQLAGVANFPYFKQTILYIMLSTPTLLIEYIYLIENQPRKMIAYGLIAYSLQVA
                     MVVLPLLVDVRVELCIFGLIGISAIRMVWLLVVVRRYADLKIDRKFMFDFIAKSTPLS
                     LKYLVSSSGLYIDQLIISAYYDSSTFAAYRFGAREIPLITILTVGLSNSMLVEFGVKE
                     HIGDTLERLRRESLKLMHILFPLSVVAIILSKPLFPVIFSSDYLQSANIFMLYALLVT
                     SRTIFPQTVTMGFQRNRFALLASTLEMVLNIALSLIFIQLFGIEGVALATVVVYLFEK
                     VLLAGYNGRRLGIHPAEYIPMKPYTAYCTLTIATYLLVRFV"
     gene            complement(1995..>2962)
                     /locus_tag="PLX53_04110"
     CDS             complement(1995..>2962)
                     /locus_tag="PLX53_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOY72296.1"
                     /translation="GVYEFSRDFRNEGMDRTHNPEFTVLEIYVAYKDYFWMMDFTEEM
                     LERVALSLHGTTAITVGDSTINFKRPFRRLTMTDAIKEYAGVDITGKTEEQLRSICKE
                     QGIEVDETMGKGKLIDTLFGEKCEQHLVQPTFIMDYPIEMSPLCKRHRSNPELTERFE
                     LIVGGKELCNAYSELNDPIDQLERFQEQLRLSEKGDDEAMFIDMDFVRALEYGMPTCS
                     GMGIGIDRLTMFMTNQPSIQDVILFPQMRPEKKQLKDPVEAYSQRNIPAEWVPVLQKL
                     GYSTVVSLTNVAPGKLFNDICGYNKKNKLGLQNPSMDDVKRWVEQ"
BASE COUNT          689 a          709 c          704 g          860 t
ORIGIN      
        1 tcgggatata atatgtttta cagctcgaaa ttatcattgt gaaaaccatg atgaaaatca
       61 cattaaagcc cttatgtctc ataatgcggt aaaattttta ttaatacatc gtttgtcgta
      121 attaaaccct attgattcgt acatctatct tgctgtatgt gtgtttttta taataaccac
      181 taactcactc gtatgtaata ccattcattt tgggtttaag aatgatgcag gcatcacatg
      241 cactcaacaa ataaatgatt attttcctta cttttcgcct gccataattc tgtgcaggac
      301 cataaggacg caaacggttt atccatgcag catggggggg cgctgcattt ccgggcatgg
      361 agtgggtgtg gaatggagca aaaaaaaacc ggagttgcct ccggttcagg ggtggaagat
      421 gggacttgaa cccacggccc ccagatccac aatctggtgc tctaaccagc tgagctacat
      481 ccaccatgtg atttaggctg caaagttata aaatttcttc aacctgaaat aaaaaaatgg
      541 ggaaactttg ttgcccatgt cctggctgcg agctgttttt ttaatacttt agcatcctgt
      601 agccattttt gatacgtaag gtgaagctag acaacacaag agcccttcag tcctaccaga
      661 ttatcaggtt tctggctgtt ttcaccattg gtatggccat ggtgaagcta tcgcttcctc
      721 aggcgcaggt tgggcagtac gaggtggtgc tgtttattgc cagcctcctt accacctttt
      781 ggattaccgg aattattcaa gcccttctcc ccatgacgtc ttcatctgtc agcagggatt
      841 cgggttccaa gcgtcctgaa attttctgcg ctctggtagt ggttgtggcg ttaacccttg
      901 tatcggcttt gctgctgctg gtttttaaaa acctgatcgg gcagctggca ggggtggcca
      961 acttccctta cttcaagcaa acaatcctgt acatcatgct gagcacccca acgctcctga
     1021 ttgagtacat ctacctgatt gaaaatcaac ctcgtaaaat gattgcctat gggcttatag
     1081 cctactcgtt gcaggtggcc atggtggtgt tgcccctgct ggttgatgtc agggttgagc
     1141 tttgcatttt cggtttgata ggcatttcgg ccatccggat ggtgtggctg ttggttgttg
     1201 ttcgccggta tgcggaccta aaaattgacc ggaagttcat gtttgatttt attgcaaaga
     1261 gcacgccgct atcgttgaag tacctggtta gcagctcggg cctctacatt gaccagctta
     1321 ttatttcagc ctactacgat tcgtcaacct ttgcagctta ccggtttggc gccagggaaa
     1381 ttccgctaat taccatactc accgttgggc tgagcaactc catgctggta gagtttgggg
     1441 taaaggagca cattggcgat accctggaga ggctcaggag ggaatcgctg aagctcatgc
     1501 acatcttgtt ccctctgtcg gtggtggcga ttatcttaag caagccccta ttcccggtga
     1561 tatttagctc cgactacttg caaagcgcca atattttcat gctttatgcc ctgctggtta
     1621 ccagcagaac tattttcccg caaacggtta ccatgggatt tcagcgaaat cgttttgcgc
     1681 tgctggcctc tacgctcgag atggtgctaa acatagcatt aagcctaata tttatacaat
     1741 tatttggaat tgaaggggtt gcgctggcta cggttgtggt ttaccttttt gaaaaggtgc
     1801 tgcttgccgg atataatggg cgtaggttgg gtattcatcc tgcagagtac ataccaatga
     1861 aaccctatac cgcctactgt acgcttacca ttgccaccta ccttttggta cggtttgtat
     1921 agcggcataa cgctacactt tcctgccatt taagaagcaa cctgcgaatt tcaccgggct
     1981 atgaattcat acacctactg ctcaacccag cgtttaacat cgtccatact tggattttgc
     2041 agccccagct tgtttttctt gttatagccg caaatgtcgt taaacagctt acccggagca
     2101 acattggtta gcgatacaac ggtgctgtag cccaacttct ggagaacagg cacccactca
     2161 gccggtatgt tcctttggga ataggcctca acagggtctt tcagctgttt tttttcaggg
     2221 cgcatctgag ggaataggat cacatcctga atggagggct ggttggtcat aaacatggtt
     2281 aaccggtcaa tcccaattcc cattcccgag caggtaggca taccatattc cagggcacga
     2341 acaaagtcca tatcaataaa catggcctcg tcgtcgccct tttcgctgag gcggagctgt
     2401 tcctgaaatc gctcaagctg gtcaatgggg tcgttgagtt cgctgtaggc gttgcaaagt
     2461 tccttgccgc ctacaatcag ctcaaaacgt tcggttagct cggggttgct acggtggcgt
     2521 ttacacaatg gcgacatctc aattgggtaa tccatgataa acgttggctg aaccaggtgc
     2581 tgctcgcact tttcaccaaa aagggtgtca atcagcttgc ctttgcccat ggtttcgtcc
     2641 acctcaatac cctgctcctt gcatatgctt cggagttgtt cctcggtttt tcccgtgata
     2701 tcaacgccgg cgtactcctt gatggcgtcg gtcatggtaa gccggcggaa gggtcgcttg
     2761 aagttgatgg tgctgtcgcc aacggttatg gctgttgttc catgcaggga taaggcaacc
     2821 cgctcaagca tctcctcggt gaagtccatc atccaaaagt agtccttgta ggccacgtaa
     2881 atttccagca cggtgaactc agggttatgg gtgcggtcca tcccctcgtt gcgaaaatcc
     2941 ctggagaact cgtaaacgcc gt
//
LOCUS       DAOOQX010000194         2960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_40262_length_2960_cov_3.336661, whole genome shotgun sequence.
ACCESSION   DAOOQX010000194 DAOOQX010000000
VERSION     DAOOQX010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40262_length_2960_cov_3.336661"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..809)
                     /locus_tag="PLX53_04115"
     CDS             complement(144..809)
                     /locus_tag="PLX53_04115"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOY72297.1"
                     /translation="MRLTKQTTSALVVITVLLIDQLLKIWIKTHFAIGDEMRVFGNWF
                     ILHFTENPGMAFGMNFWGVWGKLILSIFRLAAIVLIGWYLNHVIKQGAKTGLVVGISL
                     VMAGAIGNMIDSAFYGLLFDSGTVYNHDMNGWVSYYGVSKLNFDGYAGFLRGCVVDML
                     YFPVINTVIPSWVPIWGGHQFQFFRPVFNIADSAITTGVIYLILFQRNNIIIKDKGKG
                     VKG"
     gene            complement(895..1272)
                     /locus_tag="PLX53_04120"
     CDS             complement(895..1272)
                     /locus_tag="PLX53_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOY72298.1"
                     /translation="MGEKTRYSDEELAEFKELILQKLEKARNDYEILKSAITMSESND
                     TEDTSPTFKVLEEGASTLSKEEAGKLAQRQLKFIEHLEAALVRIENKTYGICRETGKL
                     ISKERLRAVPHATLSIDAKNNQR"
     gene            complement(1323..>2960)
                     /locus_tag="PLX53_04125"
     CDS             complement(1323..>2960)
                     /locus_tag="PLX53_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HOY72299.1"
                     /translation="SGKKMFREPDLIDVWFDSGAMPYAQLHYPFENGERFNELFPADF
                     VAEGVDQTRGWFYTLHALAALLFDSVAFKNIISNGLVLDKNGNKMSKRLGNAVVPWQV
                     IEEYGSDPLRWYMLTNSQPWDNLKFDVAGVDEVRRKFFGTLYNTYSFFALYANVDGFR
                     YAEEEVPVQNRPEIDRWILSLLNSLVREVENAYEAYEPTRAGRAIQDFVTEQLSNWYV
                     RLNRKRFWGGDYSTDKVAAYQTLYTCLETVAMLASPMAPFYMDQLFTDLNAVTGRHTD
                     QSVHLTDFPTYSNELIDKTLEEKMEIAQTVCSLVLGLRRKVNIKVRQPLSKIMIPVLD
                     ERFRVQLDSVKDLILSEVNVKELEYLSDSSGILVKKVKPNFKSLGPRFGKSMKSIATA
                     LSELSQADINTFERSGKLTLNIPEGSIDLLLSDVEVLSEDIPGWLVASEGRITVALDI
                     TITEALRLEGIARELINRVQNIRKDSGFEVTDKIRITLKKHDKLNPAVEAHYDYIAGQ
                     TLAREIILTESELNGNAIVLDLDDDLITSICVEKING"
BASE COUNT          759 a          726 c          659 g          816 t
ORIGIN      
        1 tttttaacct gaaaccctcg ctgtagcgtt taaccacata atcgttcttt gtcatgctta
       61 caattttgtg tagactcttt tcaggacggg tcacgggtca ttttttatcc tttatccttt
      121 aacctttaac cttgtattaa cctttacccc tttacccctt ttcctttgtc ctttataata
      181 atgttgttcc tttggaacag gatcaggtaa ataacccctg tggtaatggc cgaatcggca
      241 atattgaata caggacggaa gaattgaaat tggtgccctc cccaaattgg cacccagctg
      301 ggaataaccg tattaatcac tggaaagtaa agcatatcaa ccacacaccc ccttaaaaaa
      361 cctgcatagc catcaaaatt tagcttggat acgccgtagt aggaaaccca tccgttcata
      421 tcgtggttgt aaacggttcc cgaatcgaac agtaggccgt agaaagcgct gtcgatcatg
      481 ttccctatgg caccagccat taccaacgat atgccaacca ccaagcctgt tttggctccc
      541 tgtttgatga catggttcag gtaccaacca ataagaacaa tagctgccaa cctgaaaata
      601 cttaatatca gcttgcccca tactccccaa aaattcattc cgaatgccat gcccggattt
      661 tcggtaaaat gcaggataaa ccaatttcca aataccctca tctcgtcgcc aatggcaaaa
      721 tgggttttaa tccaaatttt aagtagctga tcaataagaa gaactgtaat aactaccagt
      781 gccgaggtgg tctgttttgt aagccgcata ggttagtgta aaaatttgcg caaagctact
      841 aaaaaaaaat aagcggccaa aaggccgcat ttcatggatg tatcgattaa tcagctaccg
      901 ctggttgttt ttggcgtcaa tgcttagggt ggcatggggc acagctcgta gccgctcctt
      961 ggagatcagc ttgcctgtct ccctgcagat accgtaggtt ttattctcta tgcgaaccaa
     1021 cgcagcctca agatgttcaa taaacttcag ctggcgctgg gccaactttc cggcttcctc
     1081 tttcgaaagg gttgaagctc cctcctcgag cactttaaaa gtgggcgaag tatcctcagt
     1141 atcgttgctt tcgctcatgg ttatcgcgct ttttagaatt tcatagtcgt tgcgcgcctt
     1201 ttcgagcttt tgcagaatca actccttaaa ttccgccagc tcctcatccg aatatcttgt
     1261 tttctctccc atggctgtaa aatttgctaa aattataaaa aatatcgttt caaaaaatat
     1321 cgctacccgt tgattttttc cacacaaatc gaggttatca agtcatcgtc aaggtctaaa
     1381 acgatggcgt tgccatttag ttcactttcg gttaaaatta tctccctggc cagggtttgt
     1441 ccggcaatat aatcgtaatg ggcttccacg gcagggttca acttatcgtg tttcttcagg
     1501 gtgatgcgaa ttttgtcggt tacctcaaac cccgaatcct tacgtatgtt ctgaaccctg
     1561 ttgatgagtt cacgggctat gccctcaagc cgcaaagcct ctgttatggt aatatcgaga
     1621 gctacggtta tacggccttc gctggctacc agccagccag ggatatcctc cgacagcact
     1681 tctacatcgc tgagcagcag atcaatgctt ccttctggaa tgtttaaggt taacttgccg
     1741 ctacgctcaa aggtgttgat atcggcttgt gacaactccg acagggcagt ggcaatgctt
     1801 ttcattgatt taccaaaacg cgggcctagc gatttaaaat tgggcttaac ctttttcacc
     1861 agtatgcctg acgaatcgga caggtattca agctccttta cgttaacctc gctaagaatg
     1921 aggtctttaa cggaatcaag ctgcacgcgg aacctttcat caagaacagg gatcatgatt
     1981 ttgctcagtg gctgccggac tttaatgtta accttacgac gcaggcccag aaccagcgag
     2041 cagactgtct gggcaatttc cattttttcc tccagggtct tgtctatcaa ctcattggag
     2101 tacgtgggga agtcggtcag gtgaacgctt tgatccgtat gtctcccggt tacggcgttt
     2161 aaatcggtaa agagctggtc catataaaat ggagccattg gtgatgcaag cattgccacg
     2221 gtttcgaggc aggtatagag tgtttggtaa gctgctacct tatcggtact gtaatcgcct
     2281 ccccaaaagc gcttccggtt aagccttacg taccagttgc tcagctgctc ggtaacaaaa
     2341 tcctgtattg cgcgccctgc acgggtgggt tcgtaagctt cgtaagcatt ctcaacctcc
     2401 ctaaccagcg agtttaggag cgacaaaatc cagcggtcaa tttcgggacg attttgaacg
     2461 gggacttcct cctcggcgta gcgaaatccg tcaacattag cgtaaagggc aaaaaatgag
     2521 taggtgttgt agagggtgcc aaagaatttg cgccttacct cgtcaacacc agccacgtca
     2581 aatttcaggt tgtcccaggg ttgggagttg gtgagcatgt accagcgcag cgggtcggag
     2641 ccgtactcct caataacctg ccacgggaca acggcatttc caaggcgctt gctcatcttg
     2701 ttaccattct tatcgagaac caacccgttg gagatgatat ttttgaatgc aaccgaatcg
     2761 aacagcaggg cggcaagggc atgaagggtg tagaaccacc cacgggtttg atcaacccct
     2821 tcggcaacaa aatcggcagg gaagagctcg ttgaaccttt cgccattttc gaacgggtag
     2881 tgaagctggg cgtagggcat agccccggag tcgaaccaaa cgtctatgag atcgggttcg
     2941 cgaaacatct ttttgccgct
//
LOCUS       DAOOQX010000195         2959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_40286_length_2959_cov_3.247590, whole genome shotgun sequence.
ACCESSION   DAOOQX010000195 DAOOQX010000000
VERSION     DAOOQX010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40286_length_2959_cov_3.247590"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..689)
                     /locus_tag="PLX53_04130"
     CDS             complement(66..689)
                     /locus_tag="PLX53_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOY72300.1"
                     /translation="MKLPEKTVERLSEYRRTLLSCIAKGKTHIYSHELAGIHNITAVQ
                     VRRDLMLIGYSSIKKKGYDTQELADVIGALIDHEKGLNVAVVGMGNMGRAITSYFNGK
                     RPKLNIVAAFDVDPNKVNRVIAGVNCFHMKDLLEISKRYEISIAIITSPPDSATQVAE
                     QLVSAGIKGILNFTTIPLNVPDTVYLEEFDMITSLEKVAYFVKTKDE"
     gene            complement(963..2099)
                     /locus_tag="PLX53_04135"
     CDS             complement(963..2099)
                     /locus_tag="PLX53_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOY72301.1"
                     /translation="MEVVDVLVVDDVPGIRSGVSRILKNYTINYPFLEDDISFNVVEA
                     ATGEEAIEIIQRKPPAIVLLDNKLPGIQGIDVLQFISAHEPNILVMMITSYASLELAV
                     KATNIGAHDFVPKPFTPQELRSSIENITKHYFLRQMTRKLHREGKQVRFEFLTMLSHE
                     LKAPLNAIEGYLRMMQEKQLGESLDDYQEIIDRSLSRIQSMRNLIMDLLDLTRVESGK
                     RERDLQDIDLTLVARLVVETMMPMAIQRDVTLTLESDEPVVVKADADDMEILLNNLIS
                     NAIKYNVEGGWVKCKFSKTPTYATCSVSDSGVGISADDIPKLFHEFTRIKNPLSRNVS
                     GSGLGLSIVKKIADQYGAEIKVESTLGKGSTFTLQFPVSAENPA"
     gene            complement(2106..2504)
                     /locus_tag="PLX53_04140"
     CDS             complement(2106..2504)
                     /locus_tag="PLX53_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOY72302.1"
                     /translation="MPTPAKKTILIVDDDVDYLYQLKFQVEKMGFETVTAESQHEAET
                     LLNTIKPDLAILDLMMESEDSGFILSYKLKRKYPDVPIIIATAVSAETGMTFGINTEE
                     ERKWIKADLYLEKGIRPDQLHREIVKLLKL"
     gene            complement(2532..>2959)
                     /locus_tag="PLX53_04145"
     CDS             complement(2532..>2959)
                     /locus_tag="PLX53_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY72303.1"
                     /translation="VTSVVIPKTISTQVVCKANNTEAYLDYEQMMQVLTNLNKNAVDA
                     MPNGGVLTVEVDENESDVIFRVSDTGTGIPEENMDKLFTPFFTTKGTGKGTGLGLATT
                     YGIVKMHSGKIEVDSNTDPAKGPTGTTFTIVLPRKPTLG"
BASE COUNT          790 a          664 c          643 g          862 t
ORIGIN      
        1 aacattatca aaaccatagt gtacctgcat ttaggagaca acgtttaagg ttgaagtttt
       61 accggctact catccttagt tttaacgaaa taggcaacct tttccaatga agtaatcata
      121 tcaaattctt caagataaac tgtatcgggg acatttaatg gaatggtggt aaagttaagg
      181 attcctttaa tcccggcaga aaccagctgt tcagccacct gggttgccga gtcgggcggg
      241 gaagtaatga tagcaattga aatctcatac cgtttggaaa tttcaagtaa atccttcatg
      301 tgaaagcagt taacaccagc tataacacgg ttaaccttat tcggatcaac gtcgaatgca
      361 gcaacaatat tcagctttgg ccgcttaccg ttgaagtatg aagtgatagc acgccccatg
      421 ttgcccattc ctactacggc aacatttaag cctttctcgt ggtcaataag ggcgcctatt
      481 acatcggcca actcctgggt gtcgtacccc tttttcttaa tgctggagta gccgataagc
      541 attaaatcgc gccttacctg aacagctgtg atgttatgaa ttccggccaa ctcgtgggag
      601 taaatgtgcg ttttcccttt agctatacaa cttagcaggg tacgacgata ttcactcagc
      661 ctttcaacgg ttttctcggg aagcttcatt ctattaataa aatttaaatt gacttagtaa
      721 gttcagcttt atattcatca caatccttag cctgcctatg aacataaggc tatgcatgtt
      781 ttaaaggtaa atttatatag ctttggacta gtaaactatt ttcttcgcca acttcaactc
      841 gtattgaaaa tagcgaaaat gtagaatcat gcacaaaact aaatatcttt ttaaaaatgt
      901 tacgcttttc tttaggttat gaagtaaaag aaagatatat gtttaacaaa ggtcagtgct
      961 agctaggcag ggttttcggc agataccgga aattggaggg taaaggttga gccttttccc
     1021 aacgtgcttt ccacctttat ctcggcgccg tactgatcgg caattttttt tacaatggaa
     1081 agccctagcc cacttccgct aacgttccgg ctaagggggt ttttgatgcg ggtaaactcg
     1141 tggaatagct ttggaatgtc atcagcgcta ataccaacac ccgagtcaga aacactgcag
     1201 gttgcatagg tgggggtttt gctgaattta catttcaccc atcccccctc tacgttatac
     1261 tttattgcgt tggatataag gttgttaaga agtatttcca tgtcgtcggc atcggcttta
     1321 acaactaccg gctcatcaga ttcaagggta agagtaacat cgcgctgtat ggccataggc
     1381 atcatggttt caaccaccag ccgagctacc agggtaaggt caatatcctg caagtctctt
     1441 tcccgcttac ccgattctac ccgtgtaagg tcaagcaggt ccataatcag gttgcgcatg
     1501 gactgaatac gggaaagcga gcggtcgata atttcctggt agtcatccaa actttcgcca
     1561 agctgtttct cctgcatcat tctaaggtaa ccctcaatgg cattcagtgg ggctttgagc
     1621 tcatgcgaca gcatggttaa aaactcaaac ctcacctgtt tgccttcgcg atggagtttt
     1681 cgggtcattt gacggaggaa gtagtgcttg gtaatattct cgattgacga gcgaagttcc
     1741 tgcggagtaa agggtttggg gacaaaatcg tgagcgccaa tgttggtggc tttaaccgcc
     1801 agctccaacg aagcatagga agtaatcatc ataaccagga tgttgggttc atgagcactg
     1861 atgaactgga gcacatcaat tccttgaatt cccggaagtt tattgtcaag aagaactatt
     1921 gcaggcggct tgcgctggat aatctcaata gcctcctcac ctgtggcggc ttcaaccaca
     1981 ttaaagctaa tgtcgtcctc caaaaatgga tagttaatgg tgtagttttt aagtattcgt
     2041 gagacaccag agcgaattcc tggcacatcg tcaaccacca aaacgtcaac aacctccata
     2101 tcgtgctaaa gttttaacag tttaacaatc tccctgtgaa gttggtcggg gcgaatgccc
     2161 ttttccaggt aaagatcggc cttaatccac ttccgttcct cctcggtgtt gattccgaat
     2221 gtcatcccag tttcggccga aacagcagtt gcaatgataa tgggcacatc ggggtacttc
     2281 ctttttagct tgtagcttaa tataaaacca ctatcctcgc tctccatcat taggtcgaga
     2341 atggccaggt cgggtttaat ggtgttaagc agcgtttccg cttcgtgctg gctttcggca
     2401 gttaccgttt caaaccccat tttttcaacc tgaaatttca gctggtacag gtagtcaacg
     2461 tcatcgtcaa caattaaaat ggttttcttt gcaggagtag gcatgatcca tcaatttaaa
     2521 aaattgaaca tttaacctaa agtaggtttc cggggaagca caatggtaaa agtggtacca
     2581 gtggggcctt tggcgggatc ggtgttggag tccacctcta ttttcccgct gtgcatctta
     2641 acaattccat aggtagtggc cagccccaaa ccggttcctt tgccagttcc cttggtggtg
     2701 aagaaagggg taaaaagttt atccatattc tcctcaggta tccccgttcc ggtgtcgctc
     2761 accctaaaaa ttacatcgct ttcattctca tccacctcaa cggtaagcac ccctccgttt
     2821 ggcatggcat ctacagcatt cttatttaga ttggttagaa cctgcatcat ttgttcgtaa
     2881 tcgagatagg cctcggtatt gttagccttg catactacct gagttgaaat ggtcttaggg
     2941 ataactacag aggttacgc
//
LOCUS       DAOOQX010000196         2950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_40454_length_2950_cov_3.435924, whole genome shotgun sequence.
ACCESSION   DAOOQX010000196 DAOOQX010000000
VERSION     DAOOQX010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40454_length_2950_cov_3.435924"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLX53_04150"
     CDS             complement(<1..263)
                     /locus_tag="PLX53_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72304.1"
                     /translation="MLSDFSPLEDRMFQVMDNDGRVINPKYKPNIEPERLVKAYKDML
                     FARQADLMAVSYQRQGRMYTYPPNFGQEAISGGLAVVMREQDWL"
     gene            520..1491
                     /locus_tag="PLX53_04155"
     CDS             520..1491
                     /locus_tag="PLX53_04155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY72305.1"
                     /translation="MKYSVVIPVYNRPLEVKELMESLTEQAFKDFEVLVVEDGSSIPC
                     KQEVLEYKGRLNLTYLEKENTGPGLTRNYGACSANGEYLIFFDSDCLIPNGYFQEVEK
                     ELASAPADFFGGPDRAHPSFTPIQKAISYSMTSFLTTGGIRGGKHKLDKFLPRSFNMG
                     VTAEAFRKVEGFSAMRYGEDLDLSLRLVAAGYSSRLFPGAFVYHKRRTDFRKFYRQVF
                     SSGFARINLHVRHHGSMKLVHLLPSAFVVGICLLLVASIFFPWLLMLPLLYCFALFTD
                     SLYKGNSPWVALLCIPASWVQLFGYGLGFIAACWKLTMRGKNYFLPD"
     gene            1897..2406
                     /locus_tag="PLX53_04160"
     CDS             1897..2406
                     /locus_tag="PLX53_04160"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOY72306.1"
                     /translation="MHQGNIIIAYKGSVNADIIADTLSLVEVKLNDEMEHSLTRKKVY
                     NVLVECLQNLFHHVDTVPGFYNEDNFGVFILSKIDEGFRVSTGNMVNGQKKEILKQKI
                     DKINSLDKEELKEFYKFVLNNQMFSDKGGGGLGLIDIARKTGNKLEYTFTPFDESFDF
                     FTLSATIND"
     gene            2425..2805
                     /locus_tag="PLX53_04165"
     CDS             2425..2805
                     /locus_tag="PLX53_04165"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOY72307.1"
                     /translation="MELLSIEGTPKTPTVTMNPNTGVIEIKGRSIPENSIEFYKTIID
                     WIDEYSKSPQPNTVVNIQFEYFNTSSSKCILDVFKKFEMLKKNKADVVINWYYEEDDE
                     DMLEAGEDYESIIKVPFKMIQIVD"
BASE COUNT          865 a          626 c          609 g          850 t
ORIGIN      
        1 agccaatcct gctccctcat cacaacggcc aggccacccg atatggcctc ctgcccgaaa
       61 ttaggtggat aggtgtacat gcgtccctgg cgctggtacg aaacggccat caaatcggcc
      121 tgacgagcaa aaagcatgtc cttatatgct tttaccaacc gctcgggttc aatgttgggt
      181 ttgtacttgg ggttaataac cctaccgtcg ttgtccatta cctggaacat cctatcttct
      241 aatggtgaaa aatctgaaag catactattg atgaatttat ttgtttttac gtaagtgttg
      301 gtgttctaat cgttttcatt aacaaccatt taattgaaaa tgttcattaa aacatgttga
      361 ggtgtgctga tttggcatga atagcattaa gttttcccaa ggattacaaa acaccagctt
      421 gttccacagt gattaaaatt ttataatcgt ccaatctagc tggtggaagc gggcaaaata
      481 ttttgcatta cctttacctg tctaataaaa attgcttcca tgaagtactc cgtggttatt
      541 cctgtttaca ataggccgct agaagtcaag gagcttatgg aaagccttac cgaacaggct
      601 ttcaaagatt tcgaggtgct ggtggtagag gatggttcgt caattccttg taagcaggag
      661 gttcttgaat acaaaggccg tttgaacctt acatatcttg aaaaggaaaa tacgggacca
      721 ggcttaacac gaaactacgg cgcctgttca gcaaatggtg aatatctgat atttttcgac
      781 tccgactgcc tcattcctaa tggatacttt caggaggtag agaaggagct ggcctcggct
      841 cctgctgact ttttcggagg ccccgaccgt gcacatcctt cattcacccc catacagaaa
      901 gccattagct actccatgac ctcttttctt accacaggcg gaatacgtgg aggtaagcat
      961 aaactcgaca agtttttgcc ccgtagcttt aacatgggtg ttaccgcaga agcctttaga
     1021 aaagttgaag gattttccgc catgcgctat ggcgaggacc ttgacttaag cctgcggttg
     1081 gtagcagcag gatacagctc ccgtcttttc cccggggctt ttgtttacca caagcgccgt
     1141 accgattttc gaaaattcta ccggcaggtt tttagctcgg gctttgccag gataaacctt
     1201 catgttcgac atcacggctc aatgaagctg gtgcatttgc tcccttccgc ttttgttgta
     1261 ggcatttgcc tgttgctggt cgcttctata ttttttcctt ggctgctgat gctgcctcta
     1321 ctctactgct ttgccctttt tactgactct ctatataaag gcaacagccc ttgggttgct
     1381 ttgctgtgca taccggcgtc ttgggtgcag ctgttcgggt acggcctagg gtttatagct
     1441 gcatgctgga agctaaccat gaggggtaaa aattattttt tgcctgacta agtagcaaat
     1501 ttgagtgtcg tttaaataag gtcattggtg gttgacatta taccacctct cagaagatta
     1561 gcttttcact cattctagta acataatgga catccactaa tactaacaca aagtaccttt
     1621 aaaatcagga gtaacaacct tacgcatagc tattttagta aagtcttctt tcacttatca
     1681 gccccagtag ctgaaaaaac ttcccaaacc catcaggtaa ggttgtaacc tgaagtagct
     1741 atcccaaaaa gcaaaataat aggatgcaaa aaattaagcg cctacctctt gcaactttca
     1801 aaaaaaagac agtatatttg tttagagata ccaacagctg gcaaattgct cgaaaaccaa
     1861 tccatttccg attttttaaa ccggtgttat gctagtatgc atcagggcaa tatcattatt
     1921 gcatacaagg gctcggttaa tgccgatatt attgcggata cgcttagcct ggttgaggta
     1981 aaacttaacg acgaaatgga gcatagcctt acacgaaaaa aagtttacaa cgtgttagta
     2041 gagtgccttc agaacttgtt tcaccacgtt gacacagtgc ccgggtttta caatgaggat
     2101 aattttggag tatttattct ttcaaaaatt gatgagggtt tcagggtttc aacaggcaat
     2161 atggtaaacg ggcaaaaaaa agaaattctt aagcaaaaaa ttgacaagat taattctctg
     2221 gacaaggaag aactcaaaga attttataaa tttgttctca acaatcaaat gttctctgac
     2281 aagggaggag ggggattagg gctgatagat attgcccgta aaacaggaaa caagctggag
     2341 tacaccttca caccttttga tgagtcgttt gattttttta ccctgagtgc aacaattaac
     2401 gactaacgaa ctaaaacgaa caccatggaa ttgctaagca tcgaaggaac tcctaaaaca
     2461 ccaaccgtta ccatgaaccc caacacaggg gttattgaaa taaaagggag atctattccc
     2521 gagaattcaa tagaattcta caagactatc attgattgga ttgatgagta ctcaaagtcc
     2581 ccccaaccca acacggtggt taacattcag tttgagtact tcaacacctc atcatccaag
     2641 tgcattctcg atgtatttaa gaagtttgaa atgctgaaga agaacaaggc agatgtggta
     2701 atcaactggt actacgagga ggatgacgag gacatgcttg aagcaggtga ggattacgaa
     2761 tccatcatca aggttccctt taaaatgatt cagattgttg attaaaacga cagagtatca
     2821 caattataaa gggcgtttat cgcccttttt tacttcatga actaccacgg gctaagtaca
     2881 aatttatgag ctttacggac aatcctatta aatatcaacc aatccctaca ttccatgtta
     2941 ccaattattg
//
LOCUS       DAOOQX010000197         2944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_40573_length_2944_cov_2.235029, whole genome shotgun sequence.
ACCESSION   DAOOQX010000197 DAOOQX010000000
VERSION     DAOOQX010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40573_length_2944_cov_2.235029"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PLX53_04170"
     CDS             <1..421
                     /locus_tag="PLX53_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72308.1"
                     /translation="CDHGPLGAFDGWPAEIMDALTQMGFPDKALDFYRAIEPVTYEGV
                     WSQAHELWGDEKKTKKARVRIAERGWHNRESSSGIAFSQVMLRDFFGFNPQLNGEVIQ
                     TLHGLPLSGKLYHVFYQNEYYTIELKNGKSVMIKETN"
     gene            423..1814
                     /locus_tag="PLX53_04175"
     CDS             423..1814
                     /locus_tag="PLX53_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY72309.1"
                     /translation="MMRLKVLKLVVFLICMIAEDRMILAQEFVTPAQDQFNKFTNVAN
                     GWCAADATISLLLPDGKTVWLFGDTFIGQKTGDFSISPVNSRLINNSAIIEDNGLLSA
                     YYNGTMSSPSSLIPADGESYFWPEHAIIENDTIKIFALRVTHQNNETPGFDFRIGTTR
                     IANFKYPEMEYIGSTNIQSVTDTTMRFGACVLRSGNYTYIFGVKDTTSGGMTWPLPYL
                     ARCKESINEPWEFYAGNGLWTLRCEDSKPVGDRPMSESFYVYEKDKKFYLIMHEIWTV
                     GELYILEADSITGPWNRATSGGKETLFATITPQANLFSYNLFAHPQFQLDGKILISFN
                     VNTTNFSSIYSDTRNYRARFYWLSITDAVAADYPASISLLDCYSSVPIVHPTPMSSKI
                     YFQNATGNLRFRGIDAKSTITVYGVDGRIYLNRVIYCDSDLNINHLPHGILVVKLQGD
                     WGLETQKIINHNP"
     gene            1828..2586
                     /locus_tag="PLX53_04180"
     CDS             1828..2586
                     /locus_tag="PLX53_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOY72310.1"
                     /translation="MKTISISVFMLTWCYLGIAQETTKILIDSSSSKSMTMTELPDTV
                     VKNEAKAKELEEWNKAMRAYYYHRKEQFEMLPASNKDIIFLGNSITDQCEWHELFNNM
                     NVKNRGIGGDDTDGILERLGDITKSKPNKIFIMIGTNDLAYSKTINHIVENYRKIIQS
                     IRDSSPETKIYIQSILPTNDEIHTLRKNSDIMLVNQQLEQISKEYSLTYIDLFSLFKT
                     DNNKLNPEYSLDGLHLNGKGYMVWKNAILKYVDE"
     gene            2674..>2944
                     /locus_tag="PLX53_04185"
     CDS             2674..>2944
                     /locus_tag="PLX53_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72311.1"
                     /translation="MRSSISSTLLAVFLFTGCCQEIKREVEENSLGQPEVLTFTSKPA
                     PEWTALMERTSGWFGADGIYSIPLDGVEDQSVEGKQTLFIFSDTYI"
BASE COUNT          896 a          569 c          639 g          840 t
ORIGIN      
        1 atgcgatcat ggtccattgg gcgcttttga tgggtggcct gccgaaataa tggatgccct
       61 tacccaaatg gggtttccag ataaggcact tgatttttat cgagctattg aacctgttac
      121 ctatgagggt gtttggtcgc aagcccatga actctggggt gatgagaaga aaactaaaaa
      181 ggccagagtg cgaattgctg aaagaggttg gcataaccga gaatcatcgt caggaatagc
      241 attctcacag gtaatgctac gcgatttttt cggatttaac ccgcagctta acggtgaagt
      301 tattcagact ttacatggtt tgccgttaag tggtaaactt taccatgttt tctatcaaaa
      361 tgagtactac actattgaac ttaaaaatgg gaagtcggta atgattaaag aaactaacta
      421 gcatgatgcg gctgaaagta ttaaaattag ttgttttcct gatatgtatg attgctgaag
      481 acaggatgat tctggcgcag gagtttgtta ccccagctca ggatcaattt aataagttta
      541 ccaacgttgc caacggttgg tgtgctgccg atgccaccat ttcgcttctt ctgcccgatg
      601 gtaaaaccgt atggctgttt ggcgatactt ttattggcca aaaaacgggc gatttcagca
      661 ttagtcctgt caattccagg ctgataaata actcggcaat tattgaagac aatggcttgc
      721 tttcagcata ctataacggc actatgtcca gcccatcttc attaataccc gcagatggag
      781 agagctactt ttggcccgag catgcgatta ttgagaatga taccatcaag atatttgcac
      841 ttagggttac acaccagaat aatgagactc ccggttttga ttttcgtatt ggtactactc
      901 gaatcgccaa ctttaaatat cctgaaatgg agtatatcgg gtctactaat attcaaagcg
      961 ttaccgacac aacaatgcgt tttggtgcat gtgttcttag aagcggaaac tacacttaca
     1021 tttttggtgt caaggatact acctcgggag gaatgacatg gccattaccc taccttgcaa
     1081 gatgcaagga aagcataaat gagccatggg aattttatgc ggggaatggc ctatggacgc
     1141 ttcgctgtga ggattctaaa cctgtaggcg atcgtccgat gagtgaatcc ttttatgtat
     1201 atgaaaagga taaaaagttt tacctgatta tgcatgaaat ttggacagtt ggagaacttt
     1261 acattctgga ggccgacagt ataaccggac catggaacag ggctacaagc ggagggaagg
     1321 aaactttgtt tgcaaccata acacctcaag ccaatctttt ttcctacaac ctttttgcac
     1381 atccccaatt tcagctggac ggtaaaatac ttatttcatt caacgtaaac accactaatt
     1441 tttcctcgat ttattccgac acgcggaact accgagctcg gttctactgg ctttccatca
     1501 ctgatgctgt agctgctgat tacccggcca gcatttcgtt gctcgattgt tatagtagcg
     1561 ttcctattgt tcatccaaca ccaatgtcat ccaaaattta ctttcaaaac gcaaccggaa
     1621 atcttaggtt taggggaata gacgctaaat ctaccataac tgtatatgga gttgacggac
     1681 gaatttacct taatagggtg atttattgcg attctgacct taatataaac cacctacccc
     1741 atggtatcct ggttgtaaag ttacaggggg attggggctt ggaaacacaa aaaataatta
     1801 accataaccc ttaaaattag ctatgatatg aagacaatta gtatttcagt attcatgcta
     1861 acctggtgct atctgggaat agcgcaggaa acaacaaaga tcttgattga ttcttcgtca
     1921 agtaagtcca tgaccatgac agagcttccc gatactgtag ttaaaaatga agcaaaagca
     1981 aaggagcttg aagaatggaa caaggcgatg agggcctact actaccaccg taaagagcaa
     2041 ttcgaaatgc ttccagcttc caataaagac attatttttt tagggaatag tattaccgac
     2101 cagtgtgaat ggcatgagct attcaataac atgaatgtca aaaacagggg tattggtgga
     2161 gatgatactg atggaattct tgaaaggcta ggcgatataa caaaatcgaa gcctaataaa
     2221 atatttatca tgattggcac aaacgatctg gcctactcta aaacaattaa ccacattgtt
     2281 gagaactatc ggaagattat tcaaagtatt agggattctt cgcctgaaac aaaaatatac
     2341 attcagagca ttttacctac taatgatgaa attcacaccc ttaggaaaaa ttcggatata
     2401 atgctggtta accaacagct cgagcaaatt tcgaaagaat actcacttac ctatatcgac
     2461 cttttttcgc tatttaaaac agacaataat aagttaaatc ccgagtacag ccttgatggg
     2521 cttcatctaa atggtaaagg ctatatggtg tggaagaatg ccatacttaa atatgtggat
     2581 gagtagcctg aggtgtaaaa gtggtggttc atatggtgcc acactgccag gtatagctag
     2641 gtggaaggtt tttcaattaa aatttgcagg caaatgagga gttcgatttc atcaacttta
     2701 ctggcagttt ttttgtttac cggttgctgc caggagataa agagggaggt agaagaaaat
     2761 tcattgggtc agcccgaggt cctaaccttt acaagcaaac ctgctcctga atggactgct
     2821 ttgatggagc gcacctctgg ttggtttggt gccgatggaa tctactctat tccacttgat
     2881 ggagttgaag accaaagtgt ggagggaaaa caaaccctat tcatctttag cgacacctat
     2941 attg
//
LOCUS       DAOOQX010000198         2942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_40604_length_2942_cov_3.444752, whole genome shotgun sequence.
ACCESSION   DAOOQX010000198 DAOOQX010000000
VERSION     DAOOQX010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40604_length_2942_cov_3.444752"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLX53_04190"
     CDS             complement(<1..835)
                     /locus_tag="PLX53_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOY72312.1"
                     /translation="MTLCSALVTFSMLGMAQDYKYQSVPGDPLNARIYTLANGLKVFM
                     SVSKDEPRIQTYIAVRVGSKNDPKETTGLAHYFEHMMFKGTPQFGTANWESEKVLIAK
                     IDSLFEVYRTKTDSLERARIYHVIDSISYEASKLSIPNEYDKLMTAIGSIGTNAGTSN
                     DYTIYMENIPSNQLENWAKIQAVRFANPVLRLFHTELETVYEEKNMSLTNDGRKASET
                     LLKGLFPNHPYGQQTTLGEAEHLKNPSMKNIREFYAKYYVPNNMAVVMAGDFDPDEAI
                     RI"
     gene            994..>2942
                     /locus_tag="PLX53_04195"
     CDS             994..>2942
                     /locus_tag="PLX53_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOY72313.1"
                     /translation="MKRKLKIRQKILLYILGATGLFYIACVGYILITSRQTIYKEAME
                     KTQLTAEVSAKEVTAIFERELSLVRTLSQALTVYQELPQEQWHKLFLDMYLPVLRENP
                     QIYSLWDSWEYKYYIPGYTNDFGRFVITTWREGSQLKWLYDERSMEGDPPIYGGFKRN
                     GVEAIWEPYVDQVTKGKAEVKLMITYASPVYVNSEFAGAIATDVGLDNLQEMVSKIKP
                     VTGSYAYLVSNSGIIAGHPNKEFIFKKIADVYPADEKKYQLTERIAKGEKFNYTIKDS
                     SGREHLICYSPIVAGKVKTPWALVLSTPVDVIMAQARHTLWVSIIVSLLGLIAITLVL
                     LFVSGNLTRPIKKITEALKRLARGEISEELMVSINSGDEIEEMAVAFNTSLSGLHHKT
                     LFANDIGKGNYATQLELLSEADVLGKSLITMRDSLKNAAKEEENRKLEDQKRSWANEG
                     HAHFGEILRQNNNDLQKLCDNVIISLVKYVGANQGGIFLLNDDDKMHPFFELISAYAW
                     DRKKYMTKQIELGEGLVGACAMEKETIFLTEVPDDYVNITSGLGEANPRCVILVPLKH
                     EDEVVGVVEMASFKVLEKHEVEFIEKVAESIASTILSVKINARTKMLLEQSQMQAEEM
                     KAQEEEMRQNMEELQATQEEVSRKSG"
BASE COUNT          829 a          631 c          718 g          764 t
ORIGIN      
        1 taatcctgat agcctcatcg gggtcgaaat cacccgccat aacaacagcc atgttgttgg
       61 gaacgtagta cttggcgtag aactcgcgta tgtttttcat tgatggattt ttgaggtgtt
      121 ccgcctcgcc aagggtggtt tgctgcccat agggatggtt tgggaaaagc cccttgagga
      181 gggtttcgga tgcctttcgg ccatcgttag taagcgacat gttcttttcc tcataaactg
      241 tctcaagctc ggtgtggaat aatcttaaaa cagggtttgc aaagcgaact gcctgaattt
      301 ttgcccagtt ctcgagctgg ttgctgggta tgttctccat gtagatagtg taatcgttag
      361 acgttccggc attagtgcca attgatccta ttgccgtcat aagcttatcg tactcgttgg
      421 gaatgctgag ctttgacgcc tcgtaggaaa tgctgtcaat tacatggtaa atacgtgcac
      481 gctccaagct atcggtttta gttcggtaca cctcaaaaag ggagtcaatt tttgcaataa
      541 gtaccttctc gctctcccag ttggctgtgc caaactgggg tgttccctta aacatcatgt
      601 gctcaaaata gtgggcaagc cccgttgttt ccttagggtc gtttttactt cccaccctaa
      661 cggcaatgta ggtctgaata cggggctcat ccttacttac cgacatgaat accttaagac
      721 cattagctaa ggtataaatt ctggcgttta gcgggtcgcc aggaacagac tggtatttat
      781 aatcctgtgc catgcctagc atggaaaagg taaccagcgc tgagcataaa gtcatttttt
      841 ttaaaaaaca ggtcatttct agttgggttt tggttacttc ccataagacg aaatccgccg
      901 acctaagttt aacatgttta ggcaattaaa tttcctgatg ttgttgcata gtattcaaaa
      961 atggtataag tttaaagcaa attttaaaga gccatgaagc gcaaacttaa aatccgacag
     1021 aaaatactgc tttacatttt gggagcaaca gggttattct acattgcctg tgttgggtat
     1081 atcctaatca cctcccggca aaccatctac aaggaggcga tggaaaaaac gcagcttact
     1141 gcagaagtat cggccaagga agttacggcc atttttgaac gtgagctatc gctggttcgc
     1201 accctctcgc aagctttaac ggtgtaccag gaacttccac aggagcagtg gcacaagcta
     1261 tttctggata tgtacctgcc tgtactcagg gaaaatccgc agatatactc gctttgggac
     1321 agctgggagt ataagtacta cattcccggg tacaccaatg attttggacg atttgtaatt
     1381 accacatggc gcgagggcag ccagcttaaa tggctttacg acgagcgtag catggaaggt
     1441 gatcccccaa tttatggcgg ttttaagcgt aatggcgttg aggcaatctg ggagccctac
     1501 gttgatcagg ttaccaaagg taaagccgag gttaaactga tgataaccta cgcttcacca
     1561 gtctatgtca acagtgagtt tgcaggtgct attgccaccg atgttggact agataacctg
     1621 caggaaatgg tttctaaaat taagccggtt actggtagct acgcatacct ggtatccaac
     1681 tcaggaatca ttgcaggtca tcccaacaag gagttcattt tcaagaaaat agcagatgta
     1741 tatccggccg atgaaaaaaa ataccagctt accgagcgca ttgccaaagg agaaaagttc
     1801 aactatacca taaaggattc ttcgggtcgg gaacacctga tttgctattc acctattgtg
     1861 gcaggaaagg taaaaactcc ctgggctcta gtgctttcaa cacctgttga tgttatcatg
     1921 gcacaggcca ggcataccct ttgggtttca ataattgtta gcctgcttgg cctgatagcc
     1981 attacacttg tactgctatt tgtatccgga aatctcacca gaccaatcaa aaagattacc
     2041 gaagcgttga aaaggcttgc acggggagaa atttcggaag agctgatggt tagcattaat
     2101 tcaggcgatg aaattgaaga aatggcagtt gccttcaaca cctcactgtc aggacttcac
     2161 cataaaaccc tatttgccaa cgatattgga aaagggaact acgctacaca gctcgagctg
     2221 ctgagcgagg cggatgtgct gggcaaatcg ctgataacca tgcgcgatag cttaaaaaat
     2281 gccgcaaagg aggaagagaa caggaagctg gaggaccaga agcgctcgtg ggccaacgag
     2341 ggacatgcac attttgggga aatactccgg caaaacaaca acgatttgca aaaattgtgc
     2401 gacaacgtca tcatctcgct ggttaagtat gttggagcta accagggtgg cattttcctg
     2461 ctcaacgatg atgataagat gcatccattc ttcgagctca tttcagccta tgcgtgggat
     2521 agaaaaaaat acatgaccaa gcagatagag ctgggagaag gacttgttgg cgcttgtgcc
     2581 atggaaaagg aaaccatatt ccttaccgaa gttcccgatg attacgtgaa cattacctcc
     2641 gggctaggcg aggctaaccc tcgatgtgtc attcttgtac cgcttaaaca tgaggatgaa
     2701 gtggtaggtg ttgttgaaat ggcctccttt aaagtgctcg agaagcatga ggttgagttt
     2761 atagaaaagg ttgccgaaag cattgcttcc accatccttt cggtgaagat aaacgcacgt
     2821 accaaaatgc tgctggaaca gtcgcaaatg caagccgaag agatgaaggc gcaggaggaa
     2881 gaaatgcgtc agaacatgga ggagctacaa gccacacagg aggaagtcag ccgtaaatcg
     2941 gg
//
LOCUS       DAOOQX010000199         2939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_40667_length_2939_cov_2.358530, whole genome shotgun sequence.
ACCESSION   DAOOQX010000199 DAOOQX010000000
VERSION     DAOOQX010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40667_length_2939_cov_2.358530"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2939)
                     /locus_tag="PLX53_04200"
     CDS             complement(<1..>2939)
                     /locus_tag="PLX53_04200"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOY72314.1"
                     /translation="FAFSSWKDFGNFVHSVELDAQFAGTYLKTSTLSYFAPVFHKVDI
                     AVSVDGHVKGTISDLRGKGLELRYGSQTLLRTNFNITGLPDIKQTLMVIDLYELSTNR
                     EDLAAITNPSSGKPLVNLPQNFDMLSTLTYKGNFTGYLNNFVAYGNLTSAIGSIAVDL
                     SFVPDKSNNIDINGNLRTTQLDVGKFSNSPILGKATLSASLNGTIDNQRKINFFTNAT
                     INSLQANSYTYNDIVISGNLTNKTYSGSVRLNDPNCKLNFMGIIDFSDTIPVFDFSAF
                     VPKLDLVKLNLNKKDSVSQASFLLTTKFRGSNLDNSSGEIKVVNSMYRNQHGEFKLDD
                     VTINADNKEDSKVITLQSDFAEGELRSKYNYSKFFTYLNNLVLKYIPAMKPESETESS
                     TVAFTGVEKPEYNDYLVKFRLKKTEKITSVLMPQLSIAENTSIFGILNPDLQTFSFKV
                     RVPQMRFGNTGIKDLAAEGQTKDSLFTASINTPLITIGSSKIENFKISSYLSHNLLNL
                     AIGWQNTQQPASRGLIEATADFTSYNKNGQLTSIAFNPSTFVINDSLWNLSPSTVVID
                     TASVKFNSFSIRSRNQHFNIDGKLSASNMDTLKVNLLNMDISYLNLYLQNRGYNVSGT
                     INGFAEINSAYQNPTLFSKLSINKLILNSNPIGNVTFSSTWINSEKRMAVELTNNHED
                     TLTLKATGNLYTDDGSMDFLVDVNRVKLVHLAPLLKGSVSDLSGNVNGKIRVNGKTSK
                     PMLNGNLSFSNATLTVDFLKSTFTINDKISVDNSNLYFRNFKITDRFNRVATINGDIK
                     TNSFKDINFNIGMAAENFLCMNTTEHDNSQFYGTVFGTGPIFITGKPDNLNFNINLKT
                     EGKTAIYLPISTEKTVKQNNFISFVSSNPDQIVIDEPVATKKSKVNLNLNLDLLVTPD
                     AEVQIIIDKKLGDIIKANGSGNLKMEISPEKDVFRMFGNFDIEKGDYLFTLQGVINKK
                     FTIMPGS"
BASE COUNT          699 a          553 c          732 g          955 t
ORIGIN      
        1 gctgccgggc attatggtaa atttcttgtt gattacccct tggagggtaa aaaggtagtc
       61 ccccttttcg atgtcaaagt ttccgaacat cctgaaaaca tccttttctg ggctaatttc
      121 cattttcaga tttccgctac cgttggcctt gatgatgtcg ccgagcttct tgtcaataat
      181 aatctgcacc tctgcatcgg gggttaccag caggtcgagg ttcaggttga ggtttacctt
      241 tgactttttt gtcgctacag gttcgtctat aactatttgg tcgggattgc tgctaacaaa
      301 ggaaatgaag ttgttctgct ttacagtttt ctccgttgaa atcgggaggt agatggctgt
      361 tttgccctct gtttttaggt tgatgttgaa gtttaggtta tcgggtttac cggttataaa
      421 gattggtccg gtgccaaaca ctgttccata aaactggctg ttgtcgtgct cggtagtgtt
      481 catgcagagg aaattctcgg cagccatgcc gatgttaaag ttgatatcct taaagctatt
      541 tgtcttgata tcaccgttta tggttgctac ccggttaaac cgatcggtga ttttgaaatt
      601 tctgaagtat aggttgctgt tgtcaacgga aattttgtcg tttatagtaa aggttgactt
      661 taagaaatca acagttagcg tggcgttgct aaatgagagg tttccgttaa gcattggctt
      721 gcttgttttc ccgttcaccc tgattttccc gtttacattt ccggataggt ctgaaacgct
      781 accctttagc aagggggcta ggtgaaccag cttgacacgg tttacatcta ccagaaaatc
      841 catacttcca tcgtcggtgt agaggtttcc ggttgccttc agggttagag tgtcctcatg
      901 attgttggtt agctcaactg ccatgcgctt ttctgaatta atccaggtgc tggagaaggt
      961 tacatttcca atgggatttg agtttaggat tagcttgttg atgcttagtt tggaaaaaag
     1021 cgttggattt tggtatgcac tgttgatctc ggcaaacccg ttaatggttc cgcttacgtt
     1081 gtaccctcta ttctgcaggt aaaggttaag gtatgatatg tccatattaa ggaggtttac
     1141 cttgagggta tccatgttac tggctgaaag tttaccatct atgttgaaat gctgatttct
     1201 gctgcgaatg gagaagctgt tgaattttac tgaggccgta tcaataacta ctgtcgaggg
     1261 ggacaggttc cataatgagt cgtttatcac aaatgtagat gggttaaaag caatgctggt
     1321 gagctgacca ttcttgttat aacttgtaaa atcggcagtg gcttcaatta gcccgcggga
     1381 ggcgggttgc tgggtgtttt gccagcctat ggcaagattc agcaggttat ggctaaggta
     1441 cgagctgatc ttgaaattct cgatttttga actaccaata gtaatcaacg gtgtattgat
     1501 gctggccgta aataggctgt ccttggtttg cccttcagca gctaaatctt ttatgccggt
     1561 atttccaaaa cgcatctggg gtaccctgac tttaaaagaa aaggtttgca agtcggggtt
     1621 gagaataccg aatatgctgg tgttttcggc aatgcttagc tgaggcatga gaacgctggt
     1681 aattttttcg gtttttttaa gcctgaattt aacaaggtag tcgttgtatt caggtttttc
     1741 aactcctgta aaggctacag tggaggattc tgtttcactc tccggcttca ttgccggtat
     1801 atacttcaga acgaggttgt ttaggtaggt aaagaatttt gagtagttat acttactccg
     1861 tagttctccc tcagcaaagt ccgactgaag ggtaataacc ttgctgtcct ctttattgtc
     1921 ggcatttatg gttacatcgt ctagtttaaa ttccccatgt tggtttcggt acatcgagtt
     1981 cacaacctta atttccccgc tgctgttgtc gaggttgctg cccctgaact tggtggtaag
     2041 caagaatgag gcttgggaaa ccgaatcctt tttgttgagg ttgagcttta ccaggtccag
     2101 ctttggtaca aaggctgaaa agtcgaatac gggaatcgtg tcggaaaagt caataatccc
     2161 cataaagttg agcttgcagt tgggatcgtt gagacgaacc gatccggaat aggttttgtt
     2221 tgtaaggtta cccgatatta caatgtcgtt ataggtgtag ctgtttgcct gtagggagtt
     2281 gatggttgcg ttggtgaaaa agttaatttt tctctggttg tcaatggtcc cgttgagcga
     2341 tgcgcttagt gttgcttttc caagaattgg gctgttgcta aactttccca catccagctg
     2401 tgtagttctg agattcccgt tgatgtctat gttgttgctt ttatcaggca cgaatgatag
     2461 gtcaacggca atactgccta tggcgctggt tagatttccg taggcaacaa agttgttgag
     2521 atagccggta aagttaccct tataggttag ggtgctgagc atgtcaaaat tctggggaag
     2581 gtttaccagg ggtttgccgg aagaagggtt ggtaattgca gctaaatcct cacggttggt
     2641 tgataattca tatagatcaa taaccataag ggtttgttta atgtccggga gcccggttat
     2701 gttaaagttg gttcggagta atgtttgcga tccgtacctc agctcaagtc ccttaccgcg
     2761 taagtctgaa attgtaccct ttacatggcc atcaacacta actgctatat caaccttgtg
     2821 aaacacgggt gcaaagtagg agagggtgct ggttttaagg tatgttcccg caaattgtgc
     2881 atccagctcc acgctatgga caaaattgcc aaaatccttc cagctggaaa aagcaaaat
//
LOCUS       DAOOQX010000200         2915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_41104_length_2915_cov_3.187413, whole genome shotgun sequence.
ACCESSION   DAOOQX010000200 DAOOQX010000000
VERSION     DAOOQX010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41104_length_2915_cov_3.187413"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /gene="gpmI"
                     /locus_tag="PLX53_04205"
     CDS             <1..847
                     /gene="gpmI"
                     /locus_tag="PLX53_04205"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOY72315.1"
                     /translation="VTDEFIKPIVRVDENGNPVGLIREGDVVICFNFRTDRLREITIA
                     LTQKDMPDFGMKTIPLHYVTLTRYDDTFKNVNAIYEKDNLINTLGEVVSVNGLKQLRI
                     AETEKYAHVTFFFSGGREQEFEGESRILIPSPKVATYDLQPEMSAFLVKDAIVEELKK
                     QTHDFICLNFANGDMVGHTGVYTAIQKAVQTVDTCAGEVVSTARANGYDVIIIADHGN
                     ADYALNPDGSPNTAHSLNPVPIIMVSDKFKAVDSGVLADVAPTILTLMGLTIPPEMTG
                     RVLCH"
     gene            865..1446
                     /locus_tag="PLX53_04210"
     CDS             865..1446
                     /locus_tag="PLX53_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOY72316.1"
                     /translation="MIQIDDKVVSSEILTQRFCCEIASCKGLCCVQGVSGAPLTQQEA
                     DLLDILLPAIKPFLSAKGMQTIEGHGVSVVDLDGDLVTPLVEDKECAFAIFENGAACC
                     AIEKAWRNKSIDFQKPISCHLYPIRVKQYATFTALNYDVWKVCDPARLLGDKLGLPVF
                     RFLRDAIERAYGKEFYNQLEEAGKLLSARQPNS"
     gene            1477..2847
                     /locus_tag="PLX53_04215"
     CDS             1477..2847
                     /locus_tag="PLX53_04215"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOY72317.1"
                     /translation="MDTIKSYVEANKNRFLEELFELLRIPSISTLPEHKVDMLKAAEY
                     WKKTLIEAGSDRAVVMPTAGNPVVYAEKIINPALPTVLVYAHYDVMPVDPLEQWISDP
                     FEPQIRDGKIWARGANDDKGQSFIHAKAFELMVKTNSLPCNVKFMIEGEEEVGSVSLG
                     KWCADNKELLKSDIILISDTSMMGPDTPSITVGLRGLAYVEVEVAGPSNDLHSGLFGG
                     AVANPAIILAKMIASLHDENNRITIPGFYDDVLEVSAEERAAMARAPFNLQGYKNSLD
                     IDDVHGEVGFSTIERTGIRPSLDVNGMWSGYTGNGAKTVLPSKAFAKISMRLVPNQDY
                     NKVALLFKDYFESLAPKSVKVKVNIHHGGQAYMAPITTPAYLAASLAIEDVYGKKPIP
                     FRSGGSIPIVSTFEQILGVKSLLIGFGLESDAIHSPNENFSLHQFFNGIDTIARFYGY
                     FGGHNV"
BASE COUNT          765 a          683 c          682 g          785 t
ORIGIN      
        1 cgtaaccgat gagtttatca agcccattgt cagggttgac gagaacggaa acccagttgg
       61 ccttattcgc gaaggcgatg tagttatctg ctttaacttc cgcaccgacc gtctgcgcga
      121 gattactatt gcccttactc aaaaggatat gcccgatttc ggaatgaaaa ccattccgct
      181 tcactacgtt acccttaccc gctacgacga cacttttaaa aatgtgaacg ctatttacga
      241 gaaggataac ctcatcaaca cgttgggcga ggttgtttct gttaatggcc tgaagcagct
      301 acgcattgct gaaaccgaaa aatatgccca cgttaccttc tttttctcgg gtgggaggga
      361 gcaggagttt gaaggcgaaa gccgaatact tatcccttcg cccaaggtgg ctacctacga
      421 tttgcaaccc gaaatgagtg cgtttcttgt taaggatgcc attgtggagg agcttaaaaa
      481 gcaaacccat gactttattt gccttaactt tgctaatggc gacatggtgg ggcacactgg
      541 cgtttacacg gccatccaaa aggccgttca aacggttgat acttgtgccg gggaggttgt
      601 tagcacggcc agggctaacg gatatgacgt aattattatt gccgaccatg gtaacgccga
      661 ctatgctctt aatcccgatg gttcgccaaa cacagcccat tcgcttaatc ccgtccccat
      721 tatcatggtt tccgataagt tcaaagctgt tgatagcggt gtcctggccg atgtggcacc
      781 caccattctt accctgatgg ggctgactat tcctccggaa atgaccggaa gggttttgtg
      841 ccactagcac accccgccaa catgatgatt cagattgacg acaaggtggt cagcagcgaa
      901 atacttaccc aaaggttttg ctgcgaaatt gcttcctgca agggtttatg ctgtgtccaa
      961 ggggtatcgg gcgcacctct tacccaacag gaggctgact tacttgacat tttgctccca
     1021 gccattaagc catttctttc agcaaagggc atgcaaacca ttgaagggca tggagtctct
     1081 gtggttgatt tggatggcga cctggttact cctctagttg aagacaagga atgcgccttt
     1141 gctatttttg aaaatggagc tgcatgttgt gccattgaga aggcatggag gaataaaagc
     1201 attgattttc agaagcctat ttcgtgccat ttgtacccaa ttagggtaaa acagtacgcc
     1261 acgtttaccg cacttaacta cgatgtctgg aaggtatgtg accccgcacg cttgttgggc
     1321 gataagttag ggctgcctgt tttccgtttt ttaagagatg ctattgagcg tgcctacgga
     1381 aaggagttct acaaccagct tgaagaagca ggcaagctgc tttctgcccg gcaacccaac
     1441 agctaaaatt attaaaaacc aatactttta cctgccatgg ataccataaa gtcgtatgtt
     1501 gaagccaata aaaatcgctt tttggaagaa ctgttcgagc ttcttcgcat tccatccatt
     1561 agcaccctgc cggagcataa agtcgacatg cttaaagctg ctgagtactg gaaaaaaaca
     1621 cttatagaag ctggttctga tagggctgtg gttatgccta ccgcaggcaa tcctgtggtt
     1681 tatgctgaaa aaattattaa cccagctctt cccacagttt tggtttacgc ccactacgac
     1741 gtaatgcccg ttgacccgtt agagcaatgg atttcagacc cttttgaacc ccagattcgt
     1801 gatggtaaaa tctgggcacg gggtgccaac gacgacaagg gacaatcgtt cattcatgcc
     1861 aaagcttttg aacttatggt aaaaaccaac tcccttccct gcaatgtgaa atttatgatc
     1921 gaaggggagg aggaggttgg ctcggttagc ctcgggaaat ggtgcgccga taacaaggag
     1981 ctgcttaaat ccgatatcat attaatttca gacacctcaa tgatgggtcc cgataccccc
     2041 tcaatcaccg ttggactaag gggcttagct tatgttgagg ttgaggtagc cggacccagt
     2101 aacgacttgc attccggact ttttggtgga gcagtagcta accctgccat tattttggcc
     2161 aaaatgattg cttcgctcca tgacgaaaac aaccgaatta caatacccgg tttttacgat
     2221 gatgtgctgg aggtaagcgc agaagagagg gcagcaatgg ccagggctcc cttcaacctt
     2281 cagggctata aaaactcact tgacattgac gatgttcatg gcgaagttgg attttccacc
     2341 attgaacgaa caggaattcg cccctcatta gacgtgaacg gtatgtggag cggctatacg
     2401 ggcaatggcg ctaaaaccgt tctgccatca aaagcttttg ctaaaatttc tatgcgtctt
     2461 gtccctaacc aggactacaa taaagttgcc ttgcttttta aggattactt tgaatccctg
     2521 gcgcccaaat ccgttaaggt gaaagtaaat atccatcatg gtggacaggc ctatatggct
     2581 ccaatcacaa cacccgctta ccttgctgcc agcctggcaa ttgaggatgt atatggcaaa
     2641 aagcccattc ctttcagaag tgggggaagt attccaattg tcagcacttt tgaacagatt
     2701 ttgggcgtaa aatccctact catcggcttt ggtcttgaat cggatgccat tcattcccct
     2761 aacgaaaatt tttcgctcca ccagtttttc aatggtatcg atacgattgc ccgcttttac
     2821 ggctactttg gtgggcataa cgtttagcta atcgctgctg atacctgcac ttgcaaacag
     2881 tttcatattt agggtagaaa aattttcaaa agggg
//
LOCUS       DAOOQX010000201         2903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_41326_length_2903_cov_3.350421, whole genome shotgun sequence.
ACCESSION   DAOOQX010000201 DAOOQX010000000
VERSION     DAOOQX010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41326_length_2903_cov_3.350421"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="PLX53_04220"
     CDS             complement(<1..1043)
                     /locus_tag="PLX53_04220"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOY72318.1"
                     /translation="MSRILILGAGLSSTNLIEYLLGNAPAYGWTIRIGDLSLEAAAGK
                     LGGNPNGQAFKFDIFNAEQLSAEVAWADVAISMLPAFMHPVVAKECVRQGKHLLTASY
                     VSDAMKSLHDEARQKGVALMNELGVDPGIDHMSAMRMIDGIRNKGGKITGFISNTGGL
                     IAPECDNNPWNYKFTWNPRNVVLAGQGVTHFLENGEHKYIPYHRLYSNIRTYNIPPYG
                     EFEMYPNRDSLKYREIYGINDIQTIIRGTLRRSGYSKAWDVFVQLGMTDDTYKMEGSE
                     MLTKREFLNSFLPYHPTETVEEKLQRVIPAAQDKIIFNKLKWLGIFDNELIGMANASP
                     AQLLQRIMEEKLGL"
     gene            1413..1664
                     /locus_tag="PLX53_04225"
     CDS             1413..1664
                     /locus_tag="PLX53_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOY72319.1"
                     /translation="MLTLSIGFGELVVILLVTLLVVGPREVAKVARKLGKLYRRMDNA
                     MSDLRSYVENPSSPGDNSDLVDTSEKRNDSDNANKTPAG"
     gene            1732..2385
                     /locus_tag="PLX53_04230"
     CDS             1732..2385
                     /locus_tag="PLX53_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY72320.1"
                     /translation="MIKVENITKSYGALMVLKGITLEIPEKKVVAIVGPSGAGKTTLL
                     QIMGTLGRADSGQVLINGENVAQLNDRQLAKFRNTHIGFVFQFHHLLPEFTALENVCM
                     PALIAGKGMATATKKSTELLKMLGLSDRLDHKPSELSGGEQQRVAVARALVNNPTVIL
                     ADEPSGNLDSRNKEELHKLFFMLRDQLGQTFVVVTHDKDLAQMADLQVGMNDGLLCP"
     gene            2382..>2903
                     /locus_tag="PLX53_04235"
     CDS             2382..>2903
                     /locus_tag="PLX53_04235"
                     /inference="COORDINATES: protein motif:HMM:NF021210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2400 family protein"
                     /protein_id="HOY72321.1"
                     /translation="MKLPQNRAELTQLLNNRYHRYAGASFLSDDPILIPHRFTKTEDI
                     EISALLTATIAWGTRKSIIGSMNRLMAIMDNSPFEFVINFSENDEKRLNGFAHRTFNS
                     SDCIAFIRSLQRIYADGGGLKAIFTNGFRQTRTAKQALEAFRLRLIQENFSGHAMKHV
                     PNVESGSAAKRLNM"
BASE COUNT          771 a          729 c          654 g          749 t
ORIGIN      
        1 agaccaagtt tttcctccat aattctctgc agcagctggg cggggcttgc gttggccatg
       61 ccaataagct cgttgtcaaa aatgcccagc cattttagct tgttaaaaat aatcttatcc
      121 tgagcagcag gaatcacccg ctgcagtttc tcctcaacgg tttcggtagg atgatagggt
      181 agaaaactgt tgagaaactc ccttttggtc aacatctcgc tgccctccat cttgtaggta
      241 tcgtcggtca ttcccagctg gacaaacaca tcccaggctt tgctgtagcc cgaccggcgt
      301 agcgttccgc gtataatggt ttgaatatcg ttaataccgt atatttccct gtacttaaga
      361 gagtcgcgat ttgggtacat ttcaaactcg ccataggggg gaatgttgta ggtgcgaatg
      421 ttggagtaaa gcctgtggta tggaatgtac ttatgctctc cattctcaag gaaatgcgta
      481 accccttgtc ctgcaagcac tacgttgcgt gggttccagg taaacttgta gttccatggg
      541 ttgttgtcgc actcaggagc aatcaaccct ccggtgttgc tgatgaatcc ggtaattttc
      601 cctcccttgt tcctaatacc gtcaatcatc ctcatggccg acatgtggtc aattccgggg
      661 tcaaccccca gctcgttcat caaggcaacc cctttttgtc tggcctcatc gtggagcgat
      721 ttcatggcat cggaaacgta cgatgcggtt agcaggtgct tcccctgtcg gacgcactcc
      781 ttagccacca ctgggtgcat gaatgcaggc agcatggata ttgccacatc ggcccaggcc
      841 acctcagcgc tgagctgttc agcattaaaa atatcgaact tgaaggcctg tccatttgga
      901 tttcccccaa gcttaccggc ggctgcttcc aacgaaagat caccaatacg aattgtccag
      961 ccataggctg gcgcattccc caataggtac tcaataaggt tggtggacga aagcccggca
     1021 cccagtatca aaattctact catctgcatg gcttgtatgt tgttttagtt actatttact
     1081 aaaccattta tcacctagca ttcaaacgat caagctacag acctaaccgg ttcgctgctc
     1141 ttgtatgcaa gcctgctgat gctaaaatag taaaaccagc cccgcttgct ttcagcttta
     1201 aagtttaccg ctaaatatcc tgccaatcta aaacattttt tgggaatatt tgaattattt
     1261 ttttaaaatt cacaccgttt gttaataaca agatttcctc aactttatac atgaatagat
     1321 tactgtataa tcatttcagt cagtaagaaa ttaccataga gcaaggtttt ttacatgttc
     1381 taaatcgcta tctttgacca aatggtgccg aaatgcttac cctgtcgatt ggttttggcg
     1441 agcttgtggt tatcctgctg gttaccctgc tggtggttgg ccctcgtgag gtagcaaagg
     1501 ttgccaggaa gctgggaaaa ctttaccgaa ggatggataa cgccatgtcc gatttacgca
     1561 gctacgttga aaatccctcc agcccgggtg ataacagcga tttggtagat acaagtgaaa
     1621 aaaggaacga ttcggataat gcaaacaaaa caccggcagg ataagtgcca ccacaacgtt
     1681 actttgggct aaattggtac atttgcgcag gcaaaaatct aatggatagc aatgataaag
     1741 gtcgaaaata ttaccaagtc ctacggtgca ctgatggttc taaaaggtat cacccttgaa
     1801 atacctgaga aaaaagttgt agcaattgtt ggccctagtg gcgccggtaa aacaacccta
     1861 ctacaaataa tgggtaccct gggcagagct gattccggac aagtgcttat taatggcgaa
     1921 aatgtagcac agctgaacga ccgccagctg gccaaattta ggaataccca tattggtttt
     1981 gttttccagt tccaccatct tttacccgaa ttcactgcct tagaaaacgt ttgtatgccc
     2041 gccctgattg caggaaaagg tatggcaacg gccacaaaaa agtccaccga gctgctcaag
     2101 atgcttggcc tatcggacag gctcgaccat aagccctcgg agctgtcggg aggcgagcag
     2161 caacgcgtgg ccgtggccag agcactggtt aacaatccta cggtaattct ggccgatgaa
     2221 ccctccggta acctggactc ccgaaataag gaggagctgc acaaactttt cttcatgctt
     2281 cgcgaccagc tgggccaaac ctttgtggtg gttactcacg ataaggatct tgcccaaatg
     2341 gctgacctgc aggttggcat gaacgacgga ctgctttgcc catgaaactt ccacaaaacc
     2401 gtgcagagct gacacaacta ctcaacaacc gctatcatcg ctatgccgga gcatccttcc
     2461 tttccgacga ccctatactt ataccccacc gttttactaa aaccgaggac attgagatat
     2521 ctgccctcct caccgccacc atcgcatggg gcacccgtaa aagcataata ggcagcatga
     2581 acaggcttat ggccataatg gacaacagcc cgttcgagtt cgtgataaat ttctcagaaa
     2641 acgatgagaa acggctaaac ggctttgccc atcgaacctt caactcatcc gactgcattg
     2701 cgtttattag aagtttgcag cgcatctacg ccgatggtgg aggcttaaaa gccatcttta
     2761 ccaatggctt ccggcaaaca agaacagcca agcaagcgtt ggaggcattc cggctccggt
     2821 taatccagga aaattttagc ggtcatgcca tgaagcatgt tccaaacgtg gagtcgggtt
     2881 cggcagccaa acgcctcaac atg
//
LOCUS       DAOOQX010000202         2903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_41327_length_2903_cov_3.314256, whole genome shotgun sequence.
ACCESSION   DAOOQX010000202 DAOOQX010000000
VERSION     DAOOQX010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41327_length_2903_cov_3.314256"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1899)
                     /gene="lepA"
                     /locus_tag="PLX53_04240"
     CDS             complement(112..1899)
                     /gene="lepA"
                     /locus_tag="PLX53_04240"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005806468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOY72322.1"
                     /translation="MEKIRNFCIIAHIDHGKSTLADRLLELTHTLSEREQQDQVLDSM
                     DLEREKGITIKSHAIQMVYPYGNETYTLNLIDTPGHVDFSYEVSRAIASCEGALLVVD
                     ATQGIQAQTISNLYLAINHDLEIIPVINKIDMDSAMVEDVKDQIVDLIGCKRDEILAV
                     SAKTGFGVDAVLEAIVNRIPPPKGDPEAPLQALVFDSMFNSFRGIIAYFKVVNGTIRT
                     GDRVKFVNTGFEYTADEVGILQLKLQPRNQISAGDVGYIISGIKESSEVKVGDTITHV
                     DSPCDKAIAGFEDVKPMVFAGLYPVDADEYEDLRSSLEKLKLNDASLTFEPESSLALG
                     FGFRCGFLGLLHMEIVQERLYREFDMDVITTVPNVSFRVHTTKGEIVEVHNPSGLPGP
                     TVIDYIEEPIINAQVITKSEYLGTVIKLCIDRRGVLKNQVFIASDRVELTFRLPLSEI
                     VFDFYDKLKSISRGYASFDYFLDGYQEAKLVKLDILLNGEMVDALSSLIHIDHAYDFG
                     RKMCEKLKDLIPRQQFDIAIQAAIGAKIIARETVKAVRKDVTAKCYGGDITRKRKLLE
                     KQKKGKKRMRQIGSVEVPQEAFLAVLKMD"
     gene            complement(2035..2831)
                     /gene="kdsB"
                     /locus_tag="PLX53_04245"
                     /pseudo
     CDS             complement(2035..2831)
                     /gene="kdsB"
                     /locus_tag="PLX53_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938814.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
BASE COUNT          764 a          737 c          653 g          749 t
ORIGIN      
        1 atgagctatc cagccctgct gctcactaat gtaacgccgc ataatggcag cctggtcgga
       61 tgtatatcca agccttctaa gtgcagagtg gctgttcacc gcaggaatag tttagtccat
      121 tttaagaaca gccaggaaag cctcctgtgg aacctcaacg gacccaattt ggcgcatccg
      181 ttttttaccc ttcttttgct tttcaagcag cttacgcttg cgggtaatat cgcctccgta
      241 gcattttgcc gtaacgtcct tccgaacggc cttaactgtt tcgcgggcaa taatcttggc
      301 tccaatggca gcttggatgg caatgtcgaa ctgctggcgt ggtatcaagt ccttgagttt
      361 ttcgcacatt ttacggccaa aatcgtaggc atggtcaatg tgaataagtg atgatagcgc
      421 atcaaccatc tcgccgttga gcaggatgtc gagcttaacc agcttggctt cctggtatcc
      481 atccagaaaa tagtcgaagg aggcgtaacc tcgggaaatg gatttaagct tatcgtagaa
      541 atcgaaaaca atctcgctga ggggcaaccg gaatgtgagc tccaccctat ccgaggcaat
      601 gaagacttga ttcttcagca ccccacgccg gtcaatgcaa agtttaatga cggttccaag
      661 atactccgac ttggtaataa cctgagcatt tataatcggc tcttcaatgt aatctataac
      721 ggttggacct ggtaatccgc tgggattatg cacctcaact atttctccct tggtggtgtg
      781 aacacgaaac gaaacgttgg gcacggtagt aataacatcc atgtcgaatt cacggtacag
      841 cctctcctga acaatttcca tgtgaagcag cccaagaaat ccacaacgga atccgaagcc
      901 tagtgcaagc gatgactccg gctcaaaggt gagagaggcg tcgtttagct tcagcttttc
      961 gagcgatgag cgcaagtcct catactcatc ggcatcaacc gggtagagac cagcaaaaac
     1021 cattggctta acatcctcga atcctgcaat tgccttgtcg catggcgagt caacatgggt
     1081 aatggtatcg ccaaccttca cctccgacga ctccttgata cccgatatga tgtagcccac
     1141 atcgcccgcg ctgatttggt ttcggggttg aagcttcagc tgaagaattc ccacctcgtc
     1201 ggcggtgtat tcaaagccgg tatttacgaa ctttacccta tcacccgttc gaatggtacc
     1261 gttaaccacc ttgaagtagg caataattcc cctgaaggaa ttgaacatag agtcgaaaac
     1321 taaggcctga aggggggcct caggatcgcc cttgggagga ggaatacggt ttacaatagc
     1381 ctccagtacc gcatcgactc caaaaccagt tttggcgcta accgccaaaa tttcatcacg
     1441 cttacaacct atcaagtcaa caatttgatc tttaacatcc tccaccatag ccgaatccat
     1501 atcaatcttg ttgatgaccg gaataatctc caggtcatgg ttaatggcca ggtacaggtt
     1561 ggagatggtt tgggcctgaa taccctgcgt ggcatcaaca accagcaatg caccctcgca
     1621 cgaggcaatg gcacgcgaca cctcgtatga gaaatccaca tgaccagggg tatcaatcag
     1681 gttgagggtg tatgtttcat tcccgtaagg ataaaccatc tgaatggcat ggcttttaat
     1741 ggttattccc ttttcccttt caaggtccat gctatccaac acctgatcct gctgctcgcg
     1801 ctccgacagg gtgtgagtta gctcgagcaa cctgtcggcc agggtacttt ttccatggtc
     1861 aatatgggct atgatgcaaa agtttcttat tttctccatg cacatttact gttgaagcgc
     1921 aaaggtattc aaaaaaaatg gatatgccta tgagccaccg ctatgatggc aacaaatcaa
     1981 aaaatacatt acctaatgct tctccagcaa accaacttgc atggtgggat gcaatcaggc
     2041 cgatggctgc tgcttcgtat cccttttatc cggctggttt agcaatttca tggcgaactc
     2101 cttaacccgg tttaaatcat cgggagtatc aacggcgagc gtttcctggt cggtttccag
     2161 cacacgaatg gtaaatccgt tctcaatcca tcgtagctgt tcgagcgatt cggccaactc
     2221 cagcgacgat tggggcaagc gggttatttg cctgaggata tcgggtcggt aggcgtaaag
     2281 cccaatatgc ttaaagaacc tatgggccag ctgccattcg gcctgagcct tccccctgac
     2341 gaaggggata gccgaacggc tgaagtatat ggcattgccc tctgtgctaa caactacctt
     2401 aggagcgttt gaattaaaaa tatcttcatt actaccaaag gccttaacca gcgtggcaat
     2461 ttgaaccgtt ctgtcgctaa agcactctgt aattctctgc agctgctccg gttgaataaa
     2521 aggctcgtcg ccctgaacat tgacaacaca tcaaacgaaa tatttaggct attctggagt
     2581 atttccattg cttcggcaca cctgtcggtc ccgctgcgat ggtgttcgga tgttataacc
     2641 gattgtccgc caaaaccctg aacaacatta acaattcttt gatcatcggt agccacgtaa
     2701 caatggtcaa aaacacctga tgctctttcg taaacccatt gcaccattgg ctttcctgca
     2761 ataattgcca gaggcttacc cggaaaccgg gtggaggcat acctggcagg tattagtcct
     2821 aaagttttca ttcactcggg agtaaaattt tacagaacaa ataatttaac gttatccagc
     2881 cgattacact accacaccgc aat
//
LOCUS       DAOOQX010000203         2900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_41384_length_2900_cov_3.826362, whole genome shotgun sequence.
ACCESSION   DAOOQX010000203 DAOOQX010000000
VERSION     DAOOQX010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41384_length_2900_cov_3.826362"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLX53_04250"
     CDS             <1..202
                     /locus_tag="PLX53_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72323.1"
                     /translation="LTNLAGAIIPTVSPGFRYGGVIFSMLHLKIDHKYTFFILSVWFS
                     PLLFICPFLEIKSNLQVSPTKF"
     gene            complement(321..1313)
                     /locus_tag="PLX53_04255"
     CDS             complement(321..1313)
                     /locus_tag="PLX53_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY72324.1"
                     /translation="MEQFQTWASYLAEHASDADKRKDEDPNEAPKHYIDIENYPEFIN
                     AGYIPQTLNEAITLHGSYNVYDWGILPYATKATFDSLKSNMARLNWDRAKFFAADLGH
                     YVADGHMPMHITKNYNGQLTGNDGIHSRYESTMVNAREQQLSQYEGAEIHQIDNVTQY
                     ILNYLYANYKYVDSVLAADNYATGISSNYSSTAYKNALWEKTGEFTTMLFANASHALA
                     ELMYTAWKEAGSPSLTGVDMIGQDAEGYYLYPIRPNPARNEVSISFHLEKPCVANLIV
                     CDGNGKIIATLAQGELGGGNQTITWLTRNQPNGIYLVALRANGRQFSQKLVVNH"
     gene            1926..2528
                     /locus_tag="PLX53_04260"
     CDS             1926..2528
                     /locus_tag="PLX53_04260"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOY72325.1"
                     /translation="MPNLFNRLVYSFFRASKAERSYRYGLLVASLGINTPSPIGYIEE
                     KQFGIIRRSYYVCAYQPYRATITGILGSNDTDRETIIREFTRFTFTKLHQQGVLHKDY
                     SGGNILVEDLGEDSYRFSIIDLNRMNFKKKVSTKQGLKNLRRLTLDITVNDIVTITSE
                     YAALAGNSNPKQTLFWFLKYHLDFVRFKNFKSWYKKFKRG"
BASE COUNT          807 a          623 c          585 g          885 t
ORIGIN      
        1 gctgacaaac cttgcagggg caataattcc aacagtatcg ccaggtttca ggtatggggg
       61 agtgatattc tccatgcttc atttaaaaat tgaccataaa tatacatttt ttattttatc
      121 tgtttggttc agccctttac tctttatatg cccttttctt gaaataaaat caaacctgca
      181 ggttagccca actaaatttt aagtttggca attaatgttt tacctctact gtccctcggg
      241 tgaagcaata aaaaaatccg gcgttaaact attgccggaa ttaagggtaa agcctttgcc
      301 acagcccaaa agggcaatcc ctagtggttt accaccaact tttgcgaaaa ctgcctgcca
      361 tttgccctga gagctaccag gtaaatgccg ttgggctgat tgcgggttaa ccaggttatg
      421 gtttggttgc cgccaccaag ttcaccttgc gccagggtgg caataatttt tccgtttcca
      481 tcgcaaacta ttaggtttgc cacacaaggc ttttcaagat gaaatgatat gctaacctcg
      541 ttcctggcgg gattgggcct gattgggtaa aggtagtagc cctcagcatc ttggccgatc
      601 atatctactc cggtgagcga tgggcttccg gcctccttcc atgcagtgta cattaactcg
      661 gcaagcgcat gtgaagcgtt ggcaaatagc atggtagtaa attcgccagt tttttcccaa
      721 agggcattct tatatgccgt tgagctgtag ttggacgaaa tacctgtagc gtagttgtcg
      781 gcagccagca cggaatcaac atacttataa tttgcgtaaa ggtagttcag gatgtactgc
      841 gtaacgttat caatctgatg aatttcagcc ccttcgtact gtgaaagctg ctgttccctg
      901 gcattcacca tggtgctttc gtaacgcgag tgaatgccat cgttgccggt tagctgcccg
      961 ttataatttt tagtaatgtg cattggcata tgtccatctg caacatagtg gccaaggtcg
     1021 gctgcaaaaa acttagccct atcccagttt agtctggcca tgttggattt gagcgagtca
     1081 aaagtggctt tagtggcata gggcaaaatg ccccagtcgt aaacgttata gctcccatga
     1141 agagtaattg cttcgtttag ggtttgaggg atgtatcccg cgttaataaa ctcggggtaa
     1201 ttttcaatat caatgtagtg ctttggcgct tcgttgggat cctcatcctt tcgtttatcg
     1261 gcatcggagg catgttcagc caggtagcta gcccatgtct gaaattgctc catttccaaa
     1321 ttaaaagata gcgaagattt gctactgata atgtaatgcc ccgtttgtcc ccaactgcat
     1381 agaattaacc caatggctac cagtgcaata agtctagtaa atttattcat tttttactca
     1441 gaattaacaa cgcaagttta aacatttttt aagactggtt gtttgatgga tttcatttta
     1501 ataggtttaa cattcatgga tggacgtttt aactaaaata tcatctatat ttagaaaaat
     1561 tatacactcg ccgtcttata gtaacaccta atggttcgca tcttaaatta acagtagttt
     1621 accgtaaaac actggaaagt gttgcattag agggcaaaac tttttaatat tgccaatctt
     1681 ttaatgaagc atcgagccag attttttaaa agctggatga tgcaccttaa actctacctg
     1741 ttattgagtt aatattctta cttatgatta catccgtact atcgcccgaa tttgctcatc
     1801 tacaaccatt ttttcatagt attagctcta aatttgatac tgaaggagag gttatcttta
     1861 gcaaacgtaa ccagattaaa gttttcaaca tagacggtct aaaaatctca gtaaaatgct
     1921 ttaaagtgcc taatctgttt aacagattgg tgtatagttt ttttcgagct tcaaaggccg
     1981 agcgctccta taggtatggt ttactcgtag caagtttggg cattaataca ccgtcaccta
     2041 taggctacat cgaggaaaaa caattcggaa ttattcgaag aagctactac gtgtgcgcct
     2101 atcagcccta ccgggcaacc ataacaggca tacttggatc aaatgatact gacagggaaa
     2161 cgattatcag ggaatttaca cggttcacct ttaccaaact tcaccaacag ggtgttctcc
     2221 ataaggatta ctccggaggt aatattctgg ttgaagactt gggtgaggac agctaccgct
     2281 tttcgataat cgacctgaac cgcatgaact ttaaaaagaa ggtatccacc aaacaggggc
     2341 taaaaaacct tagacggctc actctcgaca ttaccgtgaa cgatattgtt acaattacca
     2401 gcgagtatgc agctttggcc ggaaacagca accccaagca aacgctcttc tggtttttga
     2461 aataccattt agactttgtc cggttcaaga acttcaaaag ttggtataaa aaatttaaaa
     2521 ggggatgaac tgctcagcca aaacaagccg tatagattct ggttccaagg ccaacatttt
     2581 atcaatttcc acgctcttag cttccacctc gtttctattc cctatactag ctgaagaatc
     2641 atagcatctc gcctcttact tttccgggta atggtatggc tactcagtgc cttcaatacc
     2701 aagttcaccg ctttaaacca ttcgtttttt taccagcctc aaagccaacc gaccagggct
     2761 ctccttcagt gtaggtttac aattttaacc agctcattcg attgctaata aatttttctt
     2821 agtccctact tttgagttct gagcacttga cttttaaaat ttaaaagctt gtccagtggt
     2881 tttagccccg aaggggcgga
//
LOCUS       DAOOQX010000204         2893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_41538_length_2893_cov_2.453841, whole genome shotgun sequence.
ACCESSION   DAOOQX010000204 DAOOQX010000000
VERSION     DAOOQX010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41538_length_2893_cov_2.453841"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PLX53_04265"
     CDS             <1..571
                     /locus_tag="PLX53_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOY72326.1"
                     /translation="NYSALWDSIYPQMHNARMDFQKAMQLYTKGLMEMNAGKLLYPDA
                     NSTMRLTYGRVTGYKPADGLYYKPFTTLDGVMEKENPNEDVFTVDPKLRQLWQNKDYG
                     RYASNGTIPVCFLTDNDITGGNSGSPVLDGNGNLIGLAFDGNSEAMACDFMFEPETQR
                     TIAVDIRYVLFIVDKFANAQHIIGELTTL"
     gene            complement(653..1414)
                     /locus_tag="PLX53_04270"
     CDS             complement(653..1414)
                     /locus_tag="PLX53_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOY72327.1"
                     /translation="MKITFLGTGTAQGIPVIACSCPVCASTDSHDKRLRTSALIEEQG
                     INILLDAGPDFRQQMLNANVKNLNAILITHEHRDHVAGLDDVRAFNWVQRQPMDIWAE
                     DRVLEAIQREYAYIFAERKYPGIPEVELHQVFGHQFDILGVTVIPIRVYHCKLPIFGF
                     RIGDLTYITDGSFISEEEKDKIVGSRVLVINALRMQKSLSHFSLPEALKLVEELSPRK
                     AYITHISHQMGFYRDVARQLPDNVFLAYDGLVVEV"
     gene            1598..2305
                     /locus_tag="PLX53_04275"
     CDS             1598..2305
                     /locus_tag="PLX53_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOY72328.1"
                     /translation="MAGKLYLVPTTIGSTSWNEVIPSGVVELTRGLKFFVVEELRTAR
                     RYLSALGIQTPIEKLCFFTLNEHSSAADLTDMLKPLLDGNDVGLMSESGLPAIADPGS
                     MLVSAAHHHGIRVVPLCGPSSIIMTMMASGLNGQNFAFVGYLPVKSEERKRRIRQLEQ
                     RSRAENQTQLFIEAPYRNVALFRDILQTCADEARLTIAAGLTLEGEYIATKKIREWKQ
                     LPIPDINKINTVFALLF"
     gene            complement(2297..>2893)
                     /locus_tag="PLX53_04280"
     CDS             complement(2297..>2893)
                     /locus_tag="PLX53_04280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOY72329.1"
                     /translation="YMKEGGKAMLIMPSKLAYGKRSVSYTSSVIRPYQSLVYEVELHK
                     VVKNPREYELQLLHDYMNLHYPGLAPVNDSIYYIQLSPPTNDTVTLAKDSVAYVYYKG
                     MLLDGYMFDSNIDSVATRLGRSFSSTDSLKVTVGTGVIKGFSQALMLMKKGEWGRVIM
                     PSYCGYDSVGSTTIPPYSPLIFDLYFSSKSVVTKPESK"
BASE COUNT          820 a          687 c          648 g          738 t
ORIGIN      
        1 taactatagt gcgctatggg attcaattta ccctcagatg cacaacgcca gaatggattt
       61 tcagaaggct atgcagcttt acaccaaagg gttgatggag atgaatgcag gcaagctgct
      121 ttacccggat gcaaactcca ccatgcggtt aacctatggc agggttaccg gctacaagcc
      181 tgctgatggg ctttactaca agcccttcac cacacttgat ggcgtgatgg agaaggaaaa
      241 tccaaatgag gacgtcttta cggttgaccc aaaactacgt cagctttggc aaaacaagga
      301 ttatggacgt tatgcaagta acggtacaat accggtttgc tttctgaccg ataacgacat
      361 aaccggagga aactccggta gcccagtact cgacggtaat gggaatctta ttggtctggc
      421 gtttgatgga aactccgaag ccatggcctg cgacttcatg tttgagcccg agacccaacg
      481 aaccattgca gttgatatcc ggtatgtgct ttttatcgtt gataagtttg ccaatgccca
      541 gcacatcatc ggcgagctta caaccctata atacaacagc acacaagttg ttggcctgag
      601 gtgtgctttt aagctgattg caaatcaact gaagaaattc tgcacaacct tcttaaacct
      661 caacaacaag tccatcgtag gccaggaaaa cattgtcggg tagctgccgg gcaacatccc
      721 gatagaatcc catttggtgg cttatatgtg taatgtaggc ttttcgcgga ctaagctcct
      781 ctaccagctt taaggcttcg ggaagagaaa aatgcgaaag gcttttttgc atccttaaag
      841 cattaatcac cagcactctg gagcctacaa ttttatcctt ttcctcctct gaaatgaagc
      901 tgccatcggt aatgtaggtc aaatcgccta tgcgaaagcc gaatattggc agcttgcaat
      961 ggtaaacgcg aatggggatt acagtaactc caagtatgtc aaattgatgc ccaaacactt
     1021 ggtgcagctc cacttcggga ataccaggat attttcgttc ggcaaaaata taggcgtact
     1081 ccctctgaat ggcctccagc accctatctt cggcccaaat atccatgggt tgacgctgca
     1141 cccagttgaa agccctaaca tcgtccagcc cggctacatg atcgcggtgc tcatgcgtta
     1201 tcaaaatggc attaagattt ttgacgttgg cgttaagcat ttgttgccgg aaatcgggac
     1261 cggcatcaag taaaatgtta ataccttgct cctcaataag cgccgatgtt cgtaaacgct
     1321 tgtcgtgcga atcggttgag gcacacaccg gacatgaaca agctatcacg ggaataccct
     1381 gtgcggttcc agttcctaaa aaagtaatct tcactgtaaa aaaaagttag aatagctcat
     1441 attatctcag ctggcaagct aactatccaa ccacaacatc aaccagcagg aaatgttcta
     1501 tgttacttca atccagctta ccaatatccc aacgaaacgc taccttagca aaaattttca
     1561 gtaaatatag cttgttgcag taaataaaaa ctaaaggatg gccggaaaac tttacctggt
     1621 acccacgaca attggctcaa ctagttggaa cgaagtgatt ccaagcggag tggtagagct
     1681 aaccagaggg ttgaaatttt ttgtagttga agaactccgc acagctcgtc gttacctttc
     1741 agcactaggc attcaaacac ccattgaaaa gctttgtttc ttcacactta acgagcattc
     1801 ctcagctgca gacttaaccg atatgcttaa acccttgctt gatgggaatg atgttgggct
     1861 gatgtcggaa tcgggcttac cagcaatagc cgatccgggt tcaatgctgg tgtcggcagc
     1921 acaccatcat ggaattaggg ttgttccatt gtgcggcccc agctccataa tcatgaccat
     1981 gatggccagc gggctgaacg ggcaaaattt tgcctttgtt ggctacctcc ccgttaaaag
     2041 tgaggagaga aagcgacgaa taaggcagct ggagcaacgt tcgagggcgg aaaaccaaac
     2101 ccaacttttt atcgaggcac cctaccgcaa cgttgccctc ttcagggata ttctccaaac
     2161 ttgtgccgat gaggccaggc taactatagc tgcaggcctt accctggagg gagagtatat
     2221 tgccacaaaa aaaatcaggg agtggaaaca gcttcccatc cctgatatca acaagattaa
     2281 tacggttttt gccctgctat tttgactctg gtttagttac tacagactta ctgctgaagt
     2341 atagatcgaa aattagggga gagtaaggcg gtatggtggt actccctaca gaatcataac
     2401 cacagtaaga gggcataatt accctacccc attcgccctt cttcatcagc ataagcgcct
     2461 ggctaaaccc tttaataacc cctgtcccca cggttacctt aagggaatct gtagaggaaa
     2521 agcttcgtcc caacctggta gcaacactgt cgatgttgct atcgaacatg tagccatcaa
     2581 gcaacatgcc cttatagtaa acataagcaa ccgaatcctt tgccagagtt accgtgtcgt
     2641 tggttggagg cgatagctga atgtagtaaa tgctatcgtt aacaggtgct aggccagggt
     2701 agtgtagatt catgtaatcg tgaagcagct gaagctcgta ctcgcgagga tttttgacta
     2761 ccttatgcag ctcaacctca taaacaagcg attggtatgg tcttataaca gaactagtgt
     2821 atgaaacgct tcgtttcccg tatgcaagct tagagggcat aatcagcatg gcttttccgc
     2881 cttccttcat ata
//
LOCUS       DAOOQX010000205         2886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_41677_length_2886_cov_3.222536, whole genome shotgun sequence.
ACCESSION   DAOOQX010000205 DAOOQX010000000
VERSION     DAOOQX010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41677_length_2886_cov_3.222536"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..516
                     /locus_tag="PLX53_04285"
     CDS             52..516
                     /locus_tag="PLX53_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72330.1"
                     /translation="MEAKKNGLEVIEGKAGKELPFSVPDGYFDSLPRRIQERCTQPQH
                     QQPKQSWIFALRSQLALAAGFALLVVAAYFGYYTTTRSIPRDSYTLQKSDYLNIVSRS
                     ISEFDDIDLYKAIENKRKTDSIDSAARTLQQRYFIKKGNATTIIGEKYEEKP"
     gene            513..968
                     /locus_tag="PLX53_04290"
     CDS             513..968
                     /locus_tag="PLX53_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72331.1"
                     /translation="MSGHLKIFMATCWVLITVSAVSAQTANADAEKALKAEKVAFITS
                     KLELTPKEAKKFWPVYDAYWDKKNEVLRERRALTGELTKIIEQSPDDKTLLAYADRYV
                     ENRQRETELLVELNEELKEILPPRKILLLYKANHEFKNYLLQKVQETGK"
     gene            1568..>2886
                     /gene="cysS"
                     /locus_tag="PLX53_04295"
     CDS             1568..>2886
                     /gene="cysS"
                     /locus_tag="PLX53_04295"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOY72332.1"
                     /translation="MDSKLHLYNTLSRTKEEFKPINPPHVGLYVCGPTVYGAPHLGHA
                     RPAITFDLLFRYLTHLGYRVRYVRNITDVGHLENDADEGEDKIAKKARLEQLEPMEVV
                     QRYTDLYHDAMRKLNVLPPSIEPRASGHIIEQIELIKRILDNGYAYVRNGSVYFDVAR
                     YNERYPYGKLSGRRIDDLMANTRQLDGQEEKQNPFDFALWKSADPAHIMRWPSPWGEG
                     FPGWHMECSAMSIKYLGQPFDIHGGGMDLLFPHHECEVAQSVAASGHEAVRYWIHNNL
                     ITINGQKMGKSLGNFITLEELFTGSHKLLQEAYSPMTIRFFMLQAHYRSTLDFSNEAL
                     QAASKGFARLMAAIRTLDRIPPSIKSTVDVSDLQNRIYEAMNDDLNSPVAIAALFEWV
                     KIINLLAEGKETISAADLETLGSMFRTFTFDILGLVDEHQSAYKHTEL"
BASE COUNT          839 a          731 c          659 g          657 t
ORIGIN      
        1 accaaccctg acaaatatca tcgtaataga aacagttgaa ttagaatcat catggaagca
       61 aagaaaaacg ggttagaagt aattgagggc aaggcgggga aagagctccc attctctgtt
      121 ccggatggct atttcgacag cttaccccgt agaattcaag agcggtgcac ccaaccccag
      181 caccaacaac ccaaacaatc gtggattttt gccctccgat cccagctggc attagccgct
      241 ggctttgccc tgcttgttgt tgcagcgtat tttggatact acaccactac tcgctcaatt
      301 cccagggata gctacaccct tcagaaatcg gactacctaa atatcgtcag ccgttctata
      361 agcgaatttg acgatattga cctgtacaag gcaattgaaa acaaacgcaa aaccgatagc
      421 attgatagcg cagccagaac acttcagcaa cgatacttca taaaaaaagg caacgctaca
      481 accattattg gagagaaata cgaggaaaaa ccatgagtgg ccaccttaaa attttcatgg
      541 ctacctgttg ggtgctgatt accgtatcgg cggtaagcgc ccaaacagcc aatgctgatg
      601 ccgaaaaagc tctgaaggca gagaaggtag cttttatcac cagcaagctt gagctaaccc
      661 caaaggaggc aaaaaaattc tggccggttt acgatgccta ctgggataaa aaaaacgagg
      721 tgttaaggga gcgccgagcc cttaccggtg aactcactaa aataattgag cagtcgcccg
      781 acgacaaaac cctcttagct tacgccgaca ggtatgtgga aaaccgccaa agagaaaccg
      841 agttgctggt ggagctaaat gaggagctaa aggaaatact tccaccgcgt aaaattcttt
      901 tactctacaa ggcaaaccac gaatttaaaa actacctgct acaaaaggtt caggaaacag
      961 gaaagtaacc ggtggttata tttgctgcaa ccacaccttt ttgcatagta aaactttatc
     1021 cagcaattca acaggagtta ttggtacaag cgccttgttg gcctgttgct tagattactg
     1081 cttcgatttt ctcaaaaggc tgcccttagc agaaaaacgt gcaaatcagc tcgtctttct
     1141 aatccatggg taggtaaagc tcccgacgat ggttaacggc gtccattcat tgttgtccac
     1201 gaaaatgata cttcccagta aaaaaattct tgttactcca aataaaacca ccttcggtgg
     1261 ctgggggaac aattaccggg tcatcgtatc cccattcggc atttgcctaa aagttttacc
     1321 tttgcgcata ttgttacccg gctaactaat ttttacctgg taacatggtt ttcatgacac
     1381 taaaacctcc tgaattagcc atgctagcga aaattgaagc aaccaaacag gcagcacccc
     1441 atcaaggaaa atatgctaca aacccggctc aggctttgcg atgttgccgg gaaaatatta
     1501 ctgcggagta aacccatagc cgctatacat caacaagatt ttcagcctca tcaaacagca
     1561 aacacttatg gatagcaagc tgcacctata caacactcta agtagaacca aggaggaatt
     1621 taaaccgata aatccacccc atgttggatt atacgtttgc gggccaactg tttacggtgc
     1681 gccccattta gggcacgccc gaccggccat tacattcgat ttgcttttcc gctaccttac
     1741 ccacttgggg tatagggtgc gctacgtgcg taacattacc gacgttggac atctggaaaa
     1801 tgatgccgac gagggtgagg acaaaattgc aaaaaaggcg cggctggaac agctggaacc
     1861 catggaggtg gttcagcgct acaccgacct ttaccacgac gccatgcgca agcttaacgt
     1921 gctgccacca agcatagaac ccagggcctc agggcatatt attgagcaaa ttgagctgat
     1981 taaaaggata ctcgacaacg gttacgccta cgttcgcaat ggttcggttt actttgatgt
     2041 ggcccgttac aacgaacggt acccctacgg aaagctatcg ggacggagga ttgatgacct
     2101 gatggccaac acccgacagc tcgatgggca ggaggagaag cagaacccat ttgattttgc
     2161 actttggaaa agcgccgatc cggcgcacat catgcgatgg ccatcgccct ggggggaggg
     2221 atttcccgga tggcacatgg aatgctccgc tatgagcatc aagtacctgg gacaaccatt
     2281 cgacattcat ggcggtggaa tggacctgct attcccccac cacgagtgcg aagttgccca
     2341 aagcgtggct gctagtggcc acgaggcagt tcgatactgg atacacaaca acctgattac
     2401 cattaacggc cagaagatgg gcaagtcgct aggcaacttc atcaccctcg aggagctatt
     2461 caccggatcg cataagcttt tgcaggaggc gtatagcccc atgaccataa ggttcttcat
     2521 gcttcaggct cactaccgct ccaccctcga cttttcaaac gaagccctgc aggctgcctc
     2581 caaagggttt gcccgtttga tggctgccat tagaacactg gacaggattc ccccttccat
     2641 caaatcaacg gttgatgtgt ccgacctgca aaaccgcatt tatgaggcca tgaacgacga
     2701 tttgaatagc cctgtggcca ttgctgcgct atttgaatgg gtaaaaatca tcaacctgct
     2761 ggcagaaggg aaggagacta tctctgcagc cgatttggaa acgcttggct cgatgttccg
     2821 aaccttcacc ttcgacatac tgggcctagt ggacgaacat cagtcggcct acaaacatac
     2881 tgagct
//
LOCUS       DAOOQX010000206         2840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_42581_length_2840_cov_2.654937, whole genome shotgun sequence.
ACCESSION   DAOOQX010000206 DAOOQX010000000
VERSION     DAOOQX010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42581_length_2840_cov_2.654937"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..548)
                     /gene="tpx"
                     /locus_tag="PLX53_04300"
     CDS             complement(48..548)
                     /gene="tpx"
                     /locus_tag="PLX53_04300"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOY72333.1"
                     /translation="MNVTFGGKTVTLEGEQVKVGSTAPDFTALGTDLKPVKLSDFGKK
                     VKLISVFPSIDTEICSIQNHRFNNEASSFGDKVAFIAISNDLPFALKRFCGAEKITNL
                     TTLSDHKDVDFGKKFGFLIKELRLLTRGVIILDKDNKVQYVEIVPEIASEPNYTEAIA
                     RLKQLV"
     gene            complement(624..>2840)
                     /locus_tag="PLX53_04305"
     CDS             complement(624..>2840)
                     /locus_tag="PLX53_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOY72334.1"
                     /translation="HLLLAILKDDTNYVTQLLKEKDIDYERVRKNLTILNSDDKGDFG
                     SGDEDEGDSGFFGNMGKSNKPSTGKSASDTPVLDNFGIDLTKAAEDGRLDPIVGRERE
                     IERIAQILSRRKKNNPILIGEPGVGKSAIAEGLALRIVKKKVSRVLFNKRVITLDLAS
                     IVAGTKYRGQFEERMKAILNELSRTPNIILFIDEIHTIVGAGGATGSLDAANMLKPAL
                     ARGELQCIGATTLDEYREHIEKDGALERRFQKVMIEPTSPEETIEILNNIKDRYEEHH
                     NVTYTPQAIEACVKLTMRYISDRNLPDKAIDAMDEAGSRVHISNIRVPDTIVNLEKLI
                     EETKKEKIKAVKNQQFELAANLRDKEKKLIEQLEEEQSRWEKELAKHREIVDEEKVAE
                     VVAMMTGVPVQRIAQAEGARLLKMADELRNSVIGQDEAIQKVVKAIQRNRAGLKDPNK
                     PIGTFIFLGPTGVGKTQLAKVLAKYLFDTTDNLIRIDMSEYMEKFSVSRLVGAPPGYI
                     GYEEGGQLTEKVRRKPYSVVLLDEVEKAHPDVFHILLQVLDEGQLTDSLGRRVDFKNT
                     IIIMTSNIGSRELKDFGQGVGFTTKAKEQSSSSDYSRSIIEKALKKAFAPEFLNRIDD
                     IVMFNSLSRADIHKIIDIELSGLYERIRNLGFTIRISPAAKDFIAEKGYDVQYGARPL
                     KRAIQKYLEDALAEVLIKSDTKPGCTISVGYNKKKDEISIKVLKGEEEVTSASEEE"
BASE COUNT          715 a          675 c          566 g          884 t
ORIGIN      
        1 gtggaaattt ccttttattg gaaatgcacc tccaagtcga gcttttttta aactagctgc
       61 tttaggcgag ctattgcttc agtatagttg ggctcggagg ctatttcggg gactatctcc
      121 acatattgaa ctttgttatc cttgtccaga ataatcaccc ctctagtcag caaacgtagc
      181 tcctttatca aaaaaccaaa tttcttacca aaatcaacat ccttgtgatc ggatagggtg
      241 gtaaggttgg taattttttc ggctccgcag aatcgcttca aggcaaaggg taagtcattc
      301 gaaatggcaa taaaagcaac cttatcgcca aatgaagatg cttcgttatt gaagcgatga
      361 ttttgaattg agcaaatctc cgtatctata gaagggaaca cggaaattag cttaactttt
      421 ttgccaaaat cagagagctt gacaggttta aggtcggtgc cgagtgctgt aaaatcagga
      481 gcagtagagc ccactttaac ttgttcacct tcaagggtaa ctgtttttcc tccaaaagta
      541 acgttcatgg tttttatggt gtttagtgat gtaattttgg tagtaacaca gccgaaactt
      601 ttttgttcat tagccttcag gttctactcc tcttcactag cgctggttac ctcctcttcc
      661 cccttaagaa ctttgatgga aatctcatcc ttctttttgt tatatccaac ggagattgtg
      721 catccgggct tggtatcact tttaatgagc acttcggcca gggcatcctc aaggtatttt
      781 tggatggctc gtttaagagg gcgagcacca tactgaacat catatccctt ctcagcaata
      841 aaatccttgg ctgccggaga aattctaatt gtgaatccca ggttacgaat ccgctcataa
      901 aggccgctaa gctcaatatc aataatttta tgaatatcag cacgacttag cgagttgaac
      961 attacaatat catcaattct attcagaaac tccggtgcaa aggccttttt aagcgctttt
     1021 tcaattatac ttcttgaata atcgctgctg ctgctttgtt ctttggcttt ggtggtaaaa
     1081 cccacgcctt gaccaaaatc cttaagctcc cgggagccaa tgttagaagt catgatgatg
     1141 atggtattct taaaatcaac ccgtctgccc aggctatctg taagctggcc ttcatccaga
     1201 acctggagca ggatatgaaa aacgtcggga tgagcttttt caacctcatc tagcagcaca
     1261 accgagtagg gctttcttct aaccttttcg gtcagctgcc caccctcctc atatcctatg
     1321 taacctggag gtgcaccaac caaacgtgac acggaaaatt tttccatgta ctcgctcatg
     1381 tcaatcctaa taaggttatc ggtagtatca aaaagatact tggcgagcac tttagctagc
     1441 tgggttttgc caacgcctgt tggccccaaa aagataaatg tcccgatggg tttgttggga
     1501 tctttaagtc cagcacggtt tcgttggata gccttaacta ccttctgaat ggcttcatcc
     1561 tgcccaatta ccgaattcct tagctcatct gccattttga gcagtctggc tccctcagct
     1621 tgtgctattc gttgcacagg gactcctgtc atcatggcaa ccacttcggc taccttttcc
     1681 tcgtcaacaa tttcacgatg cttagccagc tccttttccc atctcgactg ctcctcctca
     1741 agctgctcaa tcagcttttt ctccttatcc ctaagattag cagccaactc aaattgttga
     1801 ttcttaacgg cttttatttt ctcctttttg gtttcctcaa tcagcttctc taggttaaca
     1861 atagtatcgg gtacacgaat attcgagata tgaacccgtg agccagcttc gtccatggca
     1921 tctatagcct tatccggtag attcctgtcg gaaatgtagc gcatggtaag ctttacacaa
     1981 gcctcaattg cctgaggtgt gtaggtaaca ttatggtgct cctcgtaccg atctttgatg
     2041 ttgttaagaa tttcgattgt ttcctcagga gaggtaggtt caatcattac tttctggaac
     2101 cgacgctcta aagccccgtc tttttcaata tgctccctat actcgtctag tgtagttgca
     2161 cctatgcact gaagctcacc ccttgcaagt gccggtttaa gcatattggc agcatcgaga
     2221 gagcccgtag ctccacctgc accaacaatg gtatgaattt catcaataaa caggatgata
     2281 ttgggagtcc tcgatagctc gttgagtatc gccttcatcc gttcttcgaa ctggccacgg
     2341 tacttggtcc cggcaacaat agaagccaga tcaagtgtga ttacccgttt gttgaaaaga
     2401 accctggata cttttttctt tacaattcga agagccagcc cttcggctat ggctgatttg
     2461 ccaactccgg gctctccaat aagaattggg ttattctttt ttcgacggct taggatttgg
     2521 gcaattcttt caatttccct ttcacggcca actataggat caagacgtcc atcctcggca
     2581 gctttagtta agtcaattcc aaaattgtca agaacggggg tgtcggaggc tgattttcct
     2641 gttgagggct tgttggattt gcccatgtta ccgaaaaaac cactatcccc ctcatcttca
     2701 tctccagaac caaaatcacc tttatcgtcc gagttcaata ttgtcagatt tttcctaacc
     2761 ctttcgtaat caatatcctt ttcctttaaa agctgggtta catagttggt atcatcctta
     2821 agtatggcaa gcaggagatg
//
LOCUS       DAOOQX010000207         2828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_42855_length_2828_cov_2.587450, whole genome shotgun sequence.
ACCESSION   DAOOQX010000207 DAOOQX010000000
VERSION     DAOOQX010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42855_length_2828_cov_2.587450"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PLX53_04310"
     CDS             complement(<1..333)
                     /locus_tag="PLX53_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72335.1"
                     /translation="MRKQVVAAFCVAGLAMASEKLIAQDFEVAPVKLMFDAEPATSQT
                     KTISVRHHSSRRVSYTVAVADFLPSNTGEKKTLPPNSTKRSCANWLNINPSFFDLNPG
                     EDIQVQVTM"
     gene            748..2616
                     /locus_tag="PLX53_04315"
     CDS             748..2616
                     /locus_tag="PLX53_04315"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY72336.1"
                     /translation="MKRILVGSTLVFALVLICPFIALSQEINVLPKQKQDDVDRYREL
                     VERYNQADNPQQAAFYLNKIAFIYWENGNVNEAIKYFLETVPLNEKIGNYTDVKAVYS
                     NVALIYADMDRLDLTLEYFNKSLDARRKLNDKGELAAGLIDVAYIHTALNQNEKAVQL
                     LDEALALSKEVNNPRLILNCLRLLSQNYDKMGNTAKANEYAGMFAVYEQQLSTQQLKT
                     EYETKVVKSEADAERERMQRTQQALLMSLRELQAKSTQDSLSYMLLQRQDSLKKAEEQ
                     ARQRQTEIDLLNTDRKLKEAQISEQKAQSRIQRIIIFFTLVFLVVVIVSTIFIYRNYR
                     DKKKANELLNLQNVEIQKQRDQIQKQNENISKSINYAQGIQKALLPPQENLQAIFPES
                     FIFFRPRDVVSGDYYWFRELSTSPGSSQKNGKVAVSAIDCTGHGVPGAFLSMIGYNLL
                     DDIVERGVHKPGAILRDLNNGIRRALRQDETDNRDGMDMALCVVDFNNKVVEFSGAKN
                     PLVYVANNEVFRLRGDKESIGGGLGYMEREFTTHSIKIEGPTWFYQFSDGFIDQFGGP
                     DGRKFMIKNFTDLLAGISILPSGQQRDILKSTFKEWLGSKYPQVDDILVVGFKVDL"
BASE COUNT          772 a          590 c          682 g          784 t
ORIGIN      
        1 catggttacc tgtacttgaa tatcttctcc tggattaagg tcgaagaacg acgggttgat
       61 gttaagccag ttggcgcagg agcgcttggt ggagttggga ggtagtgttt ttttttcacc
      121 cgtgtttgat ggcaaaaaat cggccactgc aactgtgtat gaaacccttc tgctgctgtg
      181 gtgcctaacg ctgattgttt tggtttggct ggtagcaggc tcggcatcaa acatcaattt
      241 taccggggct acctcaaaat cctgtgcaat caacttttct gatgccatcg ctaatccggc
      301 tacacaaaac gctgctacaa cttgttttct catggttgtt ggtcaatgct taagctaaca
      361 aaattattaa atatcatgct tttttgaaga actactaatc aattctgcaa aggtaaatga
      421 atttatttga tagcaggcaa aatttgatat gtaaatgatg attaatcaat gactaaggtg
      481 ttgagatatt attttatttt tcttgctcgg gttgtttcga tggcgattta accgtatccc
      541 tgcattatgg tgattgtagc tagcctcttg cttaaaacat ggctggtaat ccttgtaagg
      601 tggagcttca tagctctcta ttttaccttt agagactaca tacacatgct tgtcatcata
      661 atttccattc ttataaaaaa agtaggttta ttttgtatcg tattgaaaaa atattgtacg
      721 tttgatttaa aatcaactgg gtataaattg aaaaggattt tggtggggtc aactctggtg
      781 tttgcactgg ttttgatatg tccctttata gcgctttcgc aggaaattaa cgtgctcccc
      841 aagcaaaagc aggatgacgt tgacaggtat agggagctgg ttgagcggta caaccaagcc
      901 gataatcctc agcaggctgc tttttacctt aataagatag cctttattta ctgggagaac
      961 ggaaatgtaa acgaggctat aaagtatttt ctcgaaacgg ttcccctgaa cgaaaaaatt
     1021 ggcaactata ccgatgtaaa ggcggtttac agcaacgttg ctcttatata cgccgacatg
     1081 gaccggctcg acctgactct ggagtacttc aacaagagcc tggatgctcg gcgaaagctt
     1141 aacgataagg gcgaactagc tgcaggtttg atagatgttg catatatcca cacagccttg
     1201 aatcaaaacg aaaaggccgt gcagctgctt gatgaagcct tggccctttc gaaggaagtt
     1261 aacaaccccc ggttaattct gaactgtttg aggctgcttt cgcagaacta cgataagatg
     1321 gggaataccg ctaaggcaaa cgagtatgcc ggaatgtttg ccgtgtatga gcagcagcta
     1381 tccacccaac agcttaaaac cgagtatgaa accaaggtgg ttaagtccga agccgatgcc
     1441 gaacgtgaga gaatgcagcg aacccagcag gcgctcctca tgagcttgcg tgagcttcag
     1501 gccaagtcaa cccaggattc acttagctat atgcttttac agcgtcagga cagcctgaag
     1561 aaagctgagg agcaagcacg ccagcgccaa accgagatag atctgctgaa caccgaccgc
     1621 aagcttaaag aagctcagat tagcgagcag aaagctcagt cgcgcatcca gcgaatcatc
     1681 atatttttta cattggtctt tcttgtcgtg gtgattgttt ccacaatctt tatctacagg
     1741 aattaccggg acaagaaaaa ggccaacgag cttctcaacc tgcagaatgt tgaaattcag
     1801 aaacagcgcg atcagattca gaagcagaac gagaacatta gcaaaagcat caactatgcg
     1861 cagggaatcc agaaagccct gttaccgcca caggaaaacc tgcaggcgat tttccccgag
     1921 tcgttcatat ttttccgtcc ccgcgatgtg gtgagcggcg actactactg gttcagggag
     1981 ctatccacct cacccggctc ttcacagaaa aatgggaaag tagccgtttc ggctattgac
     2041 tgcaccgggc acggtgtacc gggtgctttt ctttccatga ttggctacaa ccttctcgac
     2101 gatattgttg agcgtggggt tcacaagcct ggggctattc ttcgtgacct gaacaacggt
     2161 atcaggcgtg ccttgcgaca ggatgaaaca gacaaccgcg atggtatgga catggccttg
     2221 tgtgtggtag attttaacaa caaggtagtt gagttttcgg gagcaaaaaa cccgcttgtt
     2281 tatgtggcaa acaacgaggt gttcaggctt cggggcgaca aggagtccat tggtggtggt
     2341 ttgggctaca tggagaggga gtttaccacc cattccatca aaatcgaggg gccaacctgg
     2401 ttctaccaat tttcggatgg attcattgac cagtttgggg gtcctgatgg tcgtaagttt
     2461 atgattaaaa actttaccga cctgctggcg ggcatttcca tccttccctc cggtcagcag
     2521 cgcgatattc tgaagagtac gtttaaagaa tggctggggt caaagtatcc tcaggttgat
     2581 gatattctgg tagttgggtt caaggttgat ttgtagcatg tagattattt tttcaattta
     2641 agctcttcag ctttcaaatt tcagccccaa cggggggaaa atattctccg cagtgctcgt
     2701 aaaggagaaa aacgttaaac gttgcaggta ttagaaaaac ggcataaaag catgaaccgc
     2761 aagtgggtcg ggtttttcag ccttgatttt ctttggttct ttcttgtatc aagacaagaa
     2821 agaacact
//
LOCUS       DAOOQX010000208         2822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_42984_length_2822_cov_3.163715, whole genome shotgun sequence.
ACCESSION   DAOOQX010000208 DAOOQX010000000
VERSION     DAOOQX010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42984_length_2822_cov_3.163715"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..2554)
                     /locus_tag="PLX53_04320"
     CDS             complement(14..2554)
                     /locus_tag="PLX53_04320"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOY72337.1"
                     /translation="MSTTIHPESLQGLSKAEAARRLIEEGYNELPSSKPKNVFQIALG
                     VVKEPMFILLVACGTLYLILGDVNEGLMLLSFVFVIMGIEFYQEKKTERALDALKNLA
                     SPRALVIRDGETVRIAGREVVTGDYVVLQEGDRVPADGVILSSINLSADESLLTGESV
                     PVRKREWLEGDGSFIPGGDDIPMVFSGSMVVNGNGVFRVTATGVNTEIGKIGKALESV
                     KEEPTRLKREMAVLVKRLAVIGVILCLLVVIIYTITRGDLLNGFLAGITLAMAMLPEE
                     FPVVLAVFMALGAWRISRNNVLTRQPAAIETLGSVTVLCTDKTGTLTQNRMTVTQLYN
                     GTNHIAVTSGDTFTEPFHQVIEYAILSSQVNPYDPMERAIISIGDKYLQNSEHIHKDW
                     VMEKEYPLSKELLAMSRVFSNTGTGRRVIAAKGAPEAIFDLCHLPAAEVGKHEKAVAE
                     MASRGLRVLGVARAELSTENLPTIQHDFNFEFMGLIGLSDPIRENVPEAVAECYRAGI
                     RVIMITGDYPVTATNIGREIGIKNPELYITGQDLETMTEEELGERIKQVNIFARVVPE
                     QKLKLVNALKRNDEIVAMTGDGVNDAPALKAAHIGIAMGEKGTDVAREASSLVLMDDN
                     FASIVKAVRMGRKISDNLQKALAYIFAIHVPIAGLSLIPVFFANLPLILWPVHIVFLE
                     LIIDPACSIIFEAEKEEKNVMTRPPRNINQPFFGVGKILMSCTQGLGILAVSLLVYFI
                     GFRMGYAEKAVRAFTFITLIVSNIAVILSNRSWSDSIFKIIMTPNKTVKWIVGGAVLF
                     LLLIVNIPALQNLFLFDKIGWLELGICTLAGLSTIIWFELYKHFKRII"
BASE COUNT          749 a          730 c          609 g          734 t
ORIGIN      
        1 ccccgaaagg tgcctatatt atccttttga aatgcttgta gagttcgaac cagataattg
       61 ttgataatcc tgccaacgtg caaatcccca actcaagcca tccaatttta tcgaacagga
      121 agaggttttg cagtgcagga atgttaacaa tcagcaataa aaacagaaca gcaccaccta
      181 caatccactt aaccgttttg ttgggagtca taataatctt gaaaatagaa tccgaccatg
      241 agcggtttga taggataact gcgatgttgg aaacaataag ggtgataaag gtaaatgctc
      301 ttacggcttt ttcggcatac cccattcgaa agcctatgaa gtaaaccagc aagctaactg
      361 caagaattcc caacccttgg gtgcagctca tcaggatttt cccgacacca aagaagggct
      421 gattgatgtt acgtggagga cgtgtcatga cattcttttc ctccttttcg gcttcaaata
      481 ttatggagca ggccgggtca atgattagct ccaggaatac gatgtgcaca ggccaaagaa
      541 taagtggcag gttggcaaag aaaaccggaa taagcgatag ccccgcaatg ggcacatgga
      601 tggcaaaaat atacgccagg gctttttgaa ggttgtcaga aatttttcgg cccatccgca
      661 ccgccttaac aatcgaggca aaattatcgt ccatcagcac cagcgaggag gcttcccggg
      721 caacatctgt tcctttctcc cccatggcaa ttccgatatg ggcggctttg agcgcagggg
      781 catcgttgac cccgtcgccg gtcatggcaa caatttcatc attccgctta agggcattta
      841 ctagcttaag cttctgttct ggaacaacac gggcaaagat gttaacctgt ttaatccgct
      901 caccaagctc ttcctccgtc atggtttcca gatcctgacc ggtgatgtaa agttcagggt
      961 ttttgatgcc tatttcccta ccaatattgg tagccgtaac aggatagtcg cccgttatca
     1021 taatcacccg gattccggct ctgtagcact cggcaaccgc ctcgggaaca ttctccctaa
     1081 ttgggtcgga gagcccaatt agccccataa actcaaagtt gaagtcatgc tgaattgtgg
     1141 gcaggttttc tgttgaaagt tcagcacgag caactccaag tactcgcagc cctctagagg
     1201 ccatttcggc caccgccttt tcgtgcttgc caacttcagc agcagggaga tggcataagt
     1261 caaatatggc ctccggagcg ccttttgccg caataaccct tctgcctgtt ccggtatttg
     1321 aaaacacccg cgacatggcc aacagctcct ttgatagcgg gtactccttc tccatcaccc
     1381 aatccttgtg gatgtgctca gaattctgca agtacttatc gccaatgctg ataattgccc
     1441 tttccatcgg gtcatagggg ttcacctggc tcgagagtat ggcatactca atcacctgat
     1501 gaaagggttc ggtaaatgtg tcgcccgatg ttacagcaat atggttagtg ccattgtaga
     1561 gctgggtaac cgtcatccgg ttttgggtaa gagtccctgt tttatcggtg cagagaactg
     1621 ttaccgagcc aagtgtttcg atggcagcag gttgacgtgt caatacgttg ttcctggata
     1681 tgcgccaggc accaagcgcc ataaaaacgg ccaaaacaac cggaaattcc tcgggaagca
     1741 tggccatagc cagggttatt ccggccaaaa acccgttgag aaggtcgcca cgggtaatgg
     1801 tgtatataat caccaccagc aagcacagga tgacaccgat tactgccagc cttttcacca
     1861 gaactgccat ctccctcttc aacctggtgg gctcttcttt cactgattcc aacgccttcc
     1921 caattttccc tatctcggta ttgaccccgg tggcggttac ccgaaacact ccattgccgt
     1981 tcaccaccat ggagcccgaa aaaaccattg gaatatcgtc ccctcccgga ataaaagagc
     2041 catctccttc gagccattcc ctttttctca ccggaacaga ctctccggtt aacaacgact
     2101 catctgctga aagattaatg gagctgagaa taaccccatc ggccggaacc ctatcgccct
     2161 cctgcaaaac aacgtaatca ccggttacaa cttcccgccc ggcaatcctt actgtttcgc
     2221 catctcttat caccaacgca cgtggactag ccaggttttt taacgcatcc agcgcccttt
     2281 cggttttttt ctcctggtag aattcaattc ccatgataac aaacacaaag ctgaggagca
     2341 taaggccttc attaacatct cctaaaataa gatacaaagt gccacaggca accagaagga
     2401 tgaacattgg ctcctttacc accccaaggg ctatctgaaa aacattcttg ggttttgagg
     2461 agggcagctc gttgtaccct tcttctataa gtcttctggc ggcttccgct tttgaaaggc
     2521 cttgcaggct ttcaggatga atagttgtgc tcattccttg tcaacttaag tcaacgtgta
     2581 tataaaaagt ccatgcttat ccgtgttgtt cgcttgagca acgcttaact tgttagatta
     2641 atctaactaa tcgggtaaaa gtgtcaagca tatcaattaa tatttcatat taaattctgg
     2701 caataaaaca aaagagtcct gttttcaaaa catggccgtt actctaccag cacttttgaa
     2761 agtaggcgtt caagcagggt tagctcctcc gccgataagc ggtttttaac catttcggca
     2821 at
//
LOCUS       DAOOQX010000209         2816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_43106_length_2816_cov_2.902934, whole genome shotgun sequence.
ACCESSION   DAOOQX010000209 DAOOQX010000000
VERSION     DAOOQX010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43106_length_2816_cov_2.902934"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1234)
                     /locus_tag="PLX53_04325"
     CDS             complement(5..1234)
                     /locus_tag="PLX53_04325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOY72338.1"
                     /translation="MKRIVILGAGTGGTIMANKLRKALDREQWEITIVDKDPVHYYQP
                     GLLFIPFGIYGKQDVVKPKASFIPAGVIFIINEIERVDPAKNRVYLANGQTLDYDFMI
                     VSTGTSIRPEETPGLLGNEWGKSIFDFYTFNGAVALQNFLKSWEGGNLVLNIAEMPYK
                     CPVAPLEFVFLADEFFVKKGIRDKVNIFYSTPLSGAFTKPKATKMLGDLLQEKNINLI
                     PDFYLERVDAENKKIISYDGRELPFDLLTIIPVNKGADFIERSSMGDDLNYISTDKYT
                     LRSEKWSNIFVLGDASNLPTSKAGSVAHFSSDVVFDNLISSIEGRPLMAKFDGHSNCY
                     IETGFGKGSLIDFNYDTEPLPGTYPVPGIGPFGLLKNTRINHYGKVMFRWMYWHLLLK
                     GKELPVDSQMSLIGKEL"
     gene            1650..2348
                     /locus_tag="PLX53_04330"
     CDS             1650..2348
                     /locus_tag="PLX53_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOY72339.1"
                     /translation="MKAEVCPGTECLTCGFRSEVFASLPDYQLEELSRSKVLMHAKKG
                     ETIARQNDSIEGFIFLRDGLAKLCRTNAEGREQIVGIATPNDFVGLLSLFSSKTYNYS
                     IVAIEDSSYCSVEYDMVMNLVGSNGEFARNLLAKISRVSDLLIGSRLDIDLLQLRGRV
                     AYILCFFANEIYHSTRFNLPVSRREIAELIDMRVENVVRILSEFRQDRIILIEGTYIE
                     ILDKEKLSWAKMHG"
     gene            complement(2356..>2816)
                     /locus_tag="PLX53_04335"
     CDS             complement(2356..>2816)
                     /locus_tag="PLX53_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY72340.1"
                     /translation="TISIAARPTADMAIAEKKERVAFCLKRVDIVGANKLYPAEISGG
                     MKKRVAIARAIVLNPEFLFCDEPNSGLDPKTAVVIDHLIKEITEEYGITTIINTHDMN
                     SVMGCGDKVMFIYNGKKWWEGTRFDVLDSDNQELNDFVFASALTRKIKGM"
BASE COUNT          713 a          620 c          646 g          837 t
ORIGIN      
        1 aaccttaaag ttcctttcct atcagggaca tttgcgaatc aaccggtaat tccttccctt
       61 ttaagagtaa gtgccagtac atccagcgga acattacttt accgtagtgg ttgatgcggg
      121 tatttttcaa gaggccgaag ggtccaatac ccggcaccgg ataggttcct ggcaggggct
      181 cggtgtcgta gttaaaatca atcagcgacc ccttgccaaa cccggtctcg atgtaacagt
      241 ttgaatggcc gtcgaatttg gccatcagcg gtctcccctc gattgacgaa atgaggttgt
      301 cgaaaactac gtccgaggag aaatgggcta ccgaaccggc ctttgatgtt ggaaggttgc
      361 tggcatcgcc cagaacaaat atattactcc atttctcgga gcgtagggtg tacttgtcgg
      421 ttgaaatata gttgaggtca tcgcccatac tgcttcgctc aatgaaatca gcgcccttat
      481 tcacaggaat aatcgttagc aggtcgaagg gcagctcccg accatcatag gagataattt
      541 ttttgttttc tgcatccacc ctttctaggt aaaagtcggg aatcaggttg atattttttt
      601 cctgaaggag gtcgcccagc atttttgttg cctttggctt ggtaaatgca cccgaaaggg
      661 gtgtagagta aaaaatgttg accttgtctc ttatcccttt ttttacgaaa aactcatcgg
      721 ccaggaaaac aaattcgagc ggtgctactg ggcatttata gggcatttcg gctatgttaa
      781 gaaccaggtt gcctccttcc cacgacttca gaaagttttg aagggcaact gccccgttga
      841 aggtgtagaa atcaaaaatg ctcttacccc actcgttacc cagcaacccg ggagtttctt
      901 ccggtctgat ggaggtgccg gttgaaacta tcatgaaatc gtaatctagc gtttgaccgt
      961 tagcgaggta aaccctattt ttagccgggt caacccgttc aatttcgttg ataataaaga
     1021 ttactcctgc agggatgaag ctggcctttg gttttactac gtcctgcttg ccgtatatcc
     1081 caaatgggat gaagagcaat cctggctgat agtaatgtac cggatcctta tccactatgg
     1141 taatttccca ttgttcccta tccaacgctt tacgaagctt gttggccatt atagtgccac
     1201 ctgttcctgc accaagtatt acgattcttt tcatacgtat atttttttag ttttcaaatc
     1261 tcctatacat taaaccatac cttttgggta tcgggggtag agctaattga acaaccattt
     1321 ttttgctttg ccaaccttgc aagtaatacc ctgttgttca ttcctgtttg tgcacgctgt
     1381 actacatgag ggcttatcac ctaaatggcc agctatttgt acttgttaat caattctaac
     1441 gctatggcgt atcttccagc agtcaaacaa tgcttctgat tggtataaca aaccatttat
     1501 gactgtttca accggctcat ttttatagca gctgtaaatg caaaggtttc cttataaatt
     1561 tcagctcaaa tttcaagtct ttttaaataa aatgtatagt tttaaatctt gttaaaaatc
     1621 aatattgata actatcaaca tatttgaata tgaaggctga agtgtgtccg ggtaccgaat
     1681 gcctgacgtg cggatttcgt tccgaggtgt ttgcttcgct tcccgattac cagctggagg
     1741 agctgtcgag gagcaaagtg ctaatgcatg ccaaaaaagg tgaaacaatt gcccgccaga
     1801 acgacagtat agagggtttt atttttttaa gggatgggct ggccaagctc tgccgcacca
     1861 acgctgaagg gagggagcaa atagtgggaa ttgctactcc aaacgatttt gttgggctgc
     1921 tcagcctttt ttcttctaaa acgtacaact actccattgt tgcaattgag gattcgagtt
     1981 actgcagcgt ggagtacgac atggtaatga atttggttgg gagcaacggg gagtttgcac
     2041 ggaatttact tgccaagatt tcgagggttt ccgatttgct gataggcagc aggctcgata
     2101 tcgatcttct tcaacttcga gggcgtgtag cctacatact ttgctttttt gccaacgaaa
     2161 tttaccattc cacacggttt aacctgccgg tttcgcgtcg ggagatagcc gagctcattg
     2221 atatgcgggt cgaaaacgtg gttagaatac tctcggagtt tagacaggat cggattattc
     2281 ttattgaggg aacttatatt gagattttag acaaagaaaa gctaagctgg gcaaaaatgc
     2341 acgggtagta tattgctaca ttccttttat tttgcgggta agtgcggagg caaaaacaaa
     2401 gtcattgagt tcctgattgt cgctatccaa cacgtcgaac ctggttcctt cccaccattt
     2461 tttaccgttg tagatgaaca tcaccttatc cccgcagccc atcacggagt tcatgtcgtg
     2521 cgtatttatg atggtagtaa taccatactc ttcggtgatt tccttaatga gatggtcaat
     2581 taccactgca gttttggggt caagtcctga gtttggctca tcgcagaaca gaaactcggg
     2641 gtttaaaacg atggcgcggg caatggctac cctctttttc atgccgccgc taatctcagc
     2701 cgggtaaagc ttgttggccc caacaatatc tacccgtttg aggcagaagg ccacccgctc
     2761 ctttttctcc gcaatggcca tgtcggcagt cggacgggca gcaatggata tggtga
//
LOCUS       DAOOQX010000210         2811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_43198_length_2811_cov_4.057692, whole genome shotgun sequence.
ACCESSION   DAOOQX010000210 DAOOQX010000000
VERSION     DAOOQX010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43198_length_2811_cov_4.057692"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="PLX53_04340"
     CDS             <1..1494
                     /locus_tag="PLX53_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72341.1"
                     /translation="SSFLAIMTLHVMSVEAQDTLVNLSSYPISNLSSKIQASSAISKS
                     KSSVQLPFFDDFSRRGVFPTDELWEVTGVTANQLYAVNPPTVGVATFDAVDFTGMLYS
                     YLTTLPTAADTITSLPINLNYLPSDSIYLSFYIQPKGLGYSPSRGDSLVVEFYSPMEG
                     AWVRAWSASVNFADSTLAFRNHLERSSTTTHSNALDRNFFRVMMGITQPEFLSDGFRF
                     RFMNYASISPSQEVQGLKSNCDHWHLDMVYLDKYRNLNDTVINDVAFFKPVKNFLRTY
                     SSVPWKHFTRAFSEEFPSPLEFSVGYRNLGDEVWNVTRRYEIVDESGAQPDYLFMGGA
                     ENIHEFQDVSYTRFFSYNFQSAWSDSAKFLLKAYLITENKSPLSLPYYLNFNDTISYN
                     LNFYNYYALDDGTAESGYGLYGEGAENGMVAVRFQNYQADSLSGVMIYFNRTYNNAND
                     IGFKITLWDERNGMPGNIIYQKSGVRPLFTDSLDRFTVYALDKVELK"
     gene            1534..1935
                     /locus_tag="PLX53_04345"
     CDS             1534..1935
                     /locus_tag="PLX53_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY72342.1"
                     /translation="MLNVGFDMNSNNSSRTLYNLNGEWVETRYNGSVMIRPIFGKLYQ
                     IPTNVDIQQPSLGFSIFPNPAQHHIRLSLPDGFTPKAIQLINTAGHVVASRPYETNEL
                     FYLEGIAPGFYMVRVSNDMGRCLTGKVIVVK"
     gene            1932..>2811
                     /locus_tag="PLX53_04350"
     CDS             1932..>2811
                     /locus_tag="PLX53_04350"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOY72343.1"
                     /translation="MIDKLHPFFNGEVDEEIDEQAELYEHFHFDVDRGQAQIRIDRFL
                     TDKIKNVSRNRIQSAADAGNIVVNGKPVKPSYKVKPLDSISIVMAYPPRDTDIKPENI
                     PLSILYEDDSLLVVDKPAGMVVHPAHGNFSGTLVNALLYHLRDVPLFSSGEVRPGLVH
                     RIDKNTSGILVVAKDEVSMNRLARQFFERTTSRKYIALVWGDMEADEGTIKGNIGRSV
                     RDRKKMQVFTDEEQGKHAVTHWRVVERFGYVTLVECRLETGRTHQIRVHFEYIHHPIF
                     NDEVYGGNKILRGTLHS"
BASE COUNT          770 a          654 c          645 g          742 t
ORIGIN      
        1 tcctcctttt tggcaattat gactctccac gtaatgtcgg tggaagctca ggatacattg
       61 gtaaatcttt cttcatatcc tataagcaat ctttcgtcta aaattcaagc aagctcagca
      121 atcagcaaat cgaaatcttc tgttcaactt ccttttttcg acgacttctc aagaagaggc
      181 gttttcccaa ctgacgagct gtgggaggta accggggtta ctgccaacca gctttatgct
      241 gttaatcccc caactgtggg cgtagccacc ttcgatgcag ttgatttcac gggtatgctt
      301 tactcatatc taaccaccct gcccacagct gccgacacca tcacctcgtt gcccattaac
      361 ctgaactacc tcccctccga cagcatctac ctttcgtttt acattcagcc caaggggttg
      421 ggctatagcc cttccagagg cgactcgttg gttgtagaat tctatagccc catggagggt
      481 gcatgggtta gagcatggtc agcttcggta aattttgccg acagcaccct ggcttttaga
      541 aaccatcttg aaagatcatc aactacgact cactccaatg ccctcgatcg caattttttt
      601 agggtgatga tgggaataac ccaacccgaa ttcctgtccg atgggttcag gtttaggttt
      661 atgaactatg catccatttc accaagccag gaggttcaag ggttgaagag caactgcgat
      721 cactggcacc tggacatggt gtacctggac aaatatagaa acctgaacga tacagttata
      781 aatgacgtcg cattttttaa acctgtaaaa aatttcctac gaacctactc atccgttccc
      841 tggaaacact ttactcgtgc atttagcgaa gagtttccta gtcctctcga gtttagtgtt
      901 ggctaccgaa atttgggcga tgaggtctgg aatgttacca ggcgctatga gattgttgac
      961 gagtcaggtg cccagcccga ctaccttttc atgggtggag ccgaaaacat ccatgagttt
     1021 caggatgtca gctatacacg ttttttttca tacaacttcc aatcggcatg gagcgattcg
     1081 gctaaattcc tcttgaaggc atacttaata acagagaata agtctccgct ctctttaccc
     1141 tactacctga actttaacga taccataagc tacaacctta atttttacaa ctactatgcc
     1201 ttggatgacg gtactgccga atcggggtac ggactatatg gcgaaggggc tgaaaatgga
     1261 atggtagccg ttaggtttca aaactaccag gccgatagcc tgagcggggt aatgatctac
     1321 ttcaaccgga cctacaacaa tgctaacgac atcgggttca agattacact ctgggatgaa
     1381 cggaacggga tgccaggcaa catcatctac cagaaatcgg gcgttagacc gctattcacc
     1441 gatagccttg acagatttac cgtttacgct ctcgataagg tggagctgaa atagggaagt
     1501 ttctatatcg gatggcagca ggagtcgagt ggtatgctca acgtcggatt cgacatgaac
     1561 agcaacaaca gcagtagaac actctacaat cttaacgggg agtgggttga aaccaggtat
     1621 aacggttcgg taatgattag accaatattt ggaaaactct atcaaatacc tactaatgtt
     1681 gacattcaac aaccttcact tgggttttcc attttcccga atcctgcaca acatcacata
     1741 cgcctatcgt tacccgatgg ctttacccca aaagctattc agctgatcaa cactgcaggt
     1801 catgttgtgg caagccgacc ctacgagacc aacgaactct tttacctcga gggtattgct
     1861 ccaggatttt acatggtcag ggtttcaaat gatatgggta ggtgcctgac cggtaaagtt
     1921 attgtggtaa aatgattgat aagctccatc cattctttaa cggtgaggtt gacgaggaga
     1981 tcgacgagca agccgaactc tacgagcatt ttcactttga tgttgatagg gggcaagccc
     2041 aaatccgaat agataggttc ctgacagata aaattaaaaa cgtcagccgc aaccgcattc
     2101 aatcagctgc cgatgctggt aatattgtgg taaatggtaa acctgttaag ccatcctaca
     2161 aggtaaaacc tcttgactcg atttcaatcg ttatggctta cccccccagg gatacggaca
     2221 tcaagccgga aaacattcca cttagcattc tgtacgagga cgattcctta ctggtggttg
     2281 ataaacctgc tggtatggta gtccatcccg cacatggcaa cttttcagga acgctggtca
     2341 acgccctact ataccatttg cgggatgttc cacttttcag cagcggcgag gtacgtccag
     2401 ggttggttca tcggatagac aagaatactt cgggaatact tgtggttgcc aaagacgagg
     2461 tgtcaatgaa ccgcctggcg cgacaattct ttgaacgaac aacttcccga aaatacattg
     2521 ccctggtatg gggggatatg gaagcggatg agggaacaat taaaggaaat attggtcgta
     2581 gtgtgcggga taggaagaaa atgcaggttt ttacagatga agagcaagga aaacatgctg
     2641 ttacccactg gcgggtggtg gagcgatttg gatatgttac ccttgtggag tgccgtctcg
     2701 aaactggtcg cacacaccag ataagggtgc actttgaata tatacatcat cccatattta
     2761 acgatgaggt gtatggagga aataagattc ttaggggcac gctccatagc c
//
LOCUS       DAOOQX010000211         2805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_43342_length_2805_cov_2.793818, whole genome shotgun sequence.
ACCESSION   DAOOQX010000211 DAOOQX010000000
VERSION     DAOOQX010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43342_length_2805_cov_2.793818"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..648
                     /locus_tag="PLX53_04355"
     CDS             190..648
                     /locus_tag="PLX53_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72344.1"
                     /translation="MQLSLMRKDEIIDFIEFNITGDSENYNRCDDESLYLNTNVFNIF
                     ASCFERSNHLFDFISPTLYNSRNFIPLRNELLHNLETLLVIKNIAEFQNYMTGIFLGK
                     DLLKVLMDYGPNFERHWRGYMRKIIQVNRHLMRLIDQCIDEERVLWVIPY"
     gene            complement(635..1732)
                     /locus_tag="PLX53_04360"
     CDS             complement(635..1732)
                     /locus_tag="PLX53_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HOY72345.1"
                     /translation="MKLVADKDIPYLRGVLEPFFEVEYIPGAGISCDVVRDAEVLLVR
                     TRTRCNADLLQHSSVRFIGSATAGFDHIDSDYCSSNGILWRNAPGCNAGGVQQWVGAA
                     IVLWLETTQRLPQSLVLGVVGVGHTGSKVAELGKALGMKVLCCDPPRTRRENLPHFVN
                     LETILSSADIISFHVPLNTTGPDKTFHMLDSDSLQRCKPNSLLINASRGEVVDSHALT
                     AFLNANPFAHAAIDVWENEPNILNELLNRCMVGTPHIAGYSLEGKVAATQMVVDAVSQ
                     HYRLELHPWQPVPNPTLAKVKLNGISSLPEAISASYNIVGDDLRRISSDFEGYRNTYS
                     YRRDFSGFEVATSDPKLKQNLTRVGFTVSRG"
     gene            1896..2339
                     /locus_tag="PLX53_04365"
     CDS             1896..2339
                     /locus_tag="PLX53_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOY72346.1"
                     /translation="MILSSLNEFGTYVNLHPLFSRIESALITMDFTHPGERIYIEGDN
                     LYATPSFSKAKAKEEALLEAHDRYIDVQVCLQGEEIMGWRNRSECLLPKEPYNEKDDI
                     TFFSDPPATFLKVPAYHFVIFYPADCHAPLIGTGLIKKVVFKVKL"
     gene            complement(2488..>2805)
                     /locus_tag="PLX53_04370"
     CDS             complement(2488..>2805)
                     /locus_tag="PLX53_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72347.1"
                     /translation="GKFSSKHVVEEIQGIRCTLVESGINKNRLDFLKSILEANGLKVC
                     FEAIPQAEGQEVLYKIGVTDLTFNPTIAIFGRRLKTPDGTVISPKYWLQETDSIKPYY
                     WLQ"
BASE COUNT          815 a          660 c          595 g          735 t
ORIGIN      
        1 agttccaagc atattatact aatccttaca aatttcattg ctaaaataca cattgcctgt
       61 tgatgtatgg gttaatatcg tccaaaaatt gtttgaaatt caataaaata ttaattttga
      121 cattaaatga gcagttgtga aaatcggaat aggcaacggt tctactagtg gagacaaact
      181 aatgtcaaaa tgcagctaag cttgatgcgc aaggatgaga tcatcgattt catcgaattc
      241 aacattacag gagactcgga aaactacaac aggtgcgacg atgagtcgct ttacctgaat
      301 acaaatgttt tcaacatatt tgccagttgc ttcgagcgga gcaaccacct ttttgatttc
      361 atatcgccca ccctttacaa cagccgcaat ttcatacctt tacgaaacga gctgctacat
      421 aacctggaaa ccttgcttgt tataaagaac attgccgagt tccaaaacta catgaccggc
      481 atttttctgg gtaaagactt actgaaggtg ctaatggact acggtccaaa ctttgagcgg
      541 cactggaggg gctacatgcg aaagataatc caggtgaacc gccacctaat gcggctaata
      601 gaccagtgca ttgacgagga gcgggtactt tgggttatcc cctactaaca gtaaatccca
      661 ccctcgtcag attttgcttt agcttaggat cgctggtggc tacctcaaaa ccgctgaagt
      721 cgcgccggta ggagtatgta ttcctgtagc cttcaaaatc tgaactaatt ctcctcaaat
      781 cgtcgccgac tatattatag ctggcgctaa tggcttcggg gagactcgaa ataccattaa
      841 gcttaacctt agccagggtg ggatttggta cgggctgcca tggatgcagt tctagccgat
      901 aatgttggct aaccgcatca accaccattt gggtggcagc taccttaccc tccagggagt
      961 accctgcaat atggggtgtg cccaccatgc aacgattcag caattcgttt agaatgttcg
     1021 gttcgttttc ccatacatca atagcagcat gggcaaaggg gttggcattt aaaaaggcag
     1081 ttaaagcatg agaatccacc acctcgcccc ttgaggcatt tataagaagg ctgtttggct
     1141 tgcatctctg taaggaatca ctatcaagca tgtggaaggt tttatcgggc ccggtagtat
     1201 tcaacggaac atggaatgag atgatgtcag cgcttgacaa aatggtttcc aggttgacaa
     1261 aatggggcaa attctcccta cgagttcgtg gcgggtcgca gcatagaact ttcattccca
     1321 gtgctttgcc aagttcagcc accttgcttc cggtatggcc aactcccacc acgcccaaaa
     1381 caagactttg aggcaacctt tgtgtcgttt ccaaccataa aacaatggct gcgccaaccc
     1441 actgctgcac accaccggca ttgcatcccg gagcattgcg ccaaagtatc ccatttgaac
     1501 tgcagtagtc agaatcaata tggtcaaatc cggctgttgc cgaaccgata aatcttacgc
     1561 tcgaatgttg aagcaaatct gcattgcacc gtgtacgggt tcgaacaagc aacacctcgg
     1621 catccctaac aacatcgcag ctaattccag ctccgggaat atactctact tcaaaaaagg
     1681 gctccaggac tcctctcagg taaggaatat ccttatcggc taccagcttc atagctccag
     1741 attttcattc agcaggcaaa gatagctaaa tggaatttcc ggttgggcca tcaaaatttt
     1801 gctcattttt agctgtgata atcacgattg tttaccttag ctaacaatta caggaacttt
     1861 tcatctaaac aaaacattgt taattccgac ctgtcatgat tctatcgagc ctaaacgagt
     1921 ttggcacata cgttaaccta catccacttt tctccagaat tgaaagcgcc ttaataacga
     1981 tggactttac acatcccggc gagaggatat acattgaggg ggataatttg tatgcaacac
     2041 cctcgttcag caaggctaaa gcaaaggaag aagccttact tgaggctcac gacaggtaca
     2101 tagacgtgca ggtttgccta cagggtgaag agattatggg ttggcgaaac cgttcggaat
     2161 gcctactacc caaagagcca tacaacgaaa aggatgatat tactttcttt tccgatccac
     2221 ctgcaacctt cctgaaagtg cctgcctacc actttgtaat tttttatccg gccgattgcc
     2281 acgccccact gataggcaca gggttaatta aaaaagtagt gttcaaggta aagctataac
     2341 ctcaactgga aaatccctgc aactggctat ccaaaacagg ccaacagaat ctaaggataa
     2401 ccatgaatga tgaaagatta ccggataact tgcaaatcca tgaggaattt aggggtttgc
     2461 aggttaacgg tgctatggaa agtctggtta ctgcaaccag tagtaaggct ttatcgaatc
     2521 cgtttcctga agccagtact ttggagagat taccgtgcca tcgggagttt tcaaccgacg
     2581 tccaaaaatg gcgatagtag gattaaatgt taagtcggtt acgccaatct tgtagagtac
     2641 ttcctgaccc tcagcctggg gaatggcctc gaagcaaacc ttcagcccat ttgcttccag
     2701 aatactttta agaaagtcta acctattctt gttgataccc gactcaacca gagtgcaacg
     2761 gataccctga atttcctcaa caacatgctt tgagctaaat ttacc
//
LOCUS       DAOOQX010000212         2802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_43408_length_2802_cov_2.646159, whole genome shotgun sequence.
ACCESSION   DAOOQX010000212 DAOOQX010000000
VERSION     DAOOQX010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43408_length_2802_cov_2.646159"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLX53_04375"
     CDS             complement(<1..570)
                     /locus_tag="PLX53_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOY72348.1"
                     /translation="MNLNNFTIKSQEAIQKAIEIAQGYNHQAVEPAHLLKAILSESEN
                     ITGYLLRKLGVNPATFDEVLDRMLDSMPKVSGGEQYLSSSTSKVLQRALEFSKSMGDQ
                     FVSVEHILLGLIVAGEQVGQMMKDAGMTEKEMKLAIEELRKGSKVNSQTAEDTYNALK
                     RFAINLNEMARSGKLDPVIGRDEEIRRVLQ"
     gene            complement(882..1331)
                     /locus_tag="PLX53_04380"
     CDS             complement(882..1331)
                     /locus_tag="PLX53_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOY72349.1"
                     /translation="MELESEVKGHFRNEYHKGLINLIYTVNLLSYEFLQSLKNHGVTE
                     QQYNVLRILRGFRSQAPVSLGFIKERMLDRNSDVSRIIDKLQRRGLVSRDVSSCDRRQ
                     KAIQITDKGLNLLSSMLDCEKKVDLLLNNLTPDELRQLNQLLDKIRG"
     gene            complement(1402..2220)
                     /locus_tag="PLX53_04385"
     CDS             complement(1402..2220)
                     /locus_tag="PLX53_04385"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HOY72350.1"
                     /translation="MNGSDKLPVIFIPHGGGPWHLIPDAMGDPVGYGNLRGYLKELGN
                     SYRSRIRSILMVSAHWEETFPTIHFGESPNLYYDYYGFPEYTYHLSWPAPGNPNLALR
                     VEQLLNNKGFNTRREMIRGYDHGTFVPMMVAFPEAQLPVVQLSLVRGLDPSTHIAVGQ
                     ALEPLRSEGVLIIGSGMSYHNMQGFMSGSPEVESTSKKFDSWLAAAVEVKNPEQRIQT
                     LVKWAEAPGAFESHPRSEHLVPLFVVAGAAGSDAGKRSYNGKLMSATISGFTFG"
     gene            complement(2225..>2802)
                     /locus_tag="PLX53_04390"
     CDS             complement(2225..>2802)
                     /locus_tag="PLX53_04390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOY72351.1"
                     /translation="LNNLEVMIKVGIIIGSTRPGRNAEAVAKWVYEHARRRTDAEFEL
                     VDIKDFNLPVLDEPVPASMHQYMKDHTKVWSDKISSFDAFIFVTPEYNHATTGALKNA
                     IDYLYVEWNNKAAGFVSYGSTGGVRAVEQLRLIMGELQVADVRTQVALSLFTDFENFS
                     VFKPAALHEDNLKAMLDQLVAWGGALKTIRK"
BASE COUNT          742 a          702 c          601 g          757 t
ORIGIN      
        1 ctgcagtacc ctccttatct cctcatcacg accaatcacc ggatcgagct tcccgcttcg
       61 cgccatctcg ttgaggttaa tggcaaatct cttaagcgca ttataggtgt cctcggctgt
      121 ttgtgaattg acctttgatc cttttctcaa ctcctcaata gccagcttca tctccttttc
      181 ggtcattccg gcatccttca tcatctgtcc aacctgttca ccggccacaa ttaaccccaa
      241 caggatatgc tccacggaca cgaactggtc gcccatggac ttgctaaatt ccagtgccct
      301 ttgcagcacc ttgctggttg acgagcttag gtactgctcg ccacccgaaa cctttggcat
      361 ggaatcaagc atccggtcca gcacctcatc aaatgttgca gggtttacac ccagctttcg
      421 aagaaggtac cctgtaatgt tttcggactc actcaaaata gcctttagca agtgcgcagg
      481 ttctacagct tgatggttgt acccctgcgc aatctcaatg gccttctgaa tggcctcctg
      541 cgattttatg gtaaaattat tcaagttcat actatagttc ctccttaaat ttatcaccat
      601 aagccagcaa aacatctacc ataacccaaa taatgccatg ttgacataag cacaaagcgc
      661 aaataggaaa ttatgacagt gcggagaaat caggaatatc cctaattttg cgtaatgcac
      721 tatattcaag cacattataa taaaaaacaa ccgtaagccc acttcgggat tttacggttt
      781 aaacggttcc agcatgatga acagccagaa tggatggcgt tcaaaaagtt ttctgataca
      841 gaataagaaa aacagtcggg aagtgtatga tttagggcag gtcagcccct aattttgtcg
      901 aggagctgat taagctgcct caattcgtcg ggagtgagat tatttagtaa caggtcaacc
      961 tttttttcac agtcgagcat gctgctaagc aagtttaaac ccttatcagt aatctgaatg
     1021 gctttctggc gtctgtcgca actgctgaca tctctggata ccaggccccg cctttgaagt
     1081 ttgtcaataa tccggctaac atcagaattc ctatcgagca ttcgttcctt aataaatcca
     1141 agcgatacgg gtgcctggct tctaaagcca cgtaaaattc taagcacgtt gtactgctgc
     1201 tcggtaactc catggttttt aagcgactgg agaaactcgt agctcagcag gttcaccgta
     1261 taaatcaaat ttataagtcc cttatggtat tcgttcctaa aatggccctt tacttcgctt
     1321 tccagctcca tgttttaaag aggattaagt ttctgaaaaa aaacgacgcc acaatttagt
     1381 cttatgcttg cccaaggatt attaaccaaa tgtaaagccc gaaatggtgg cgctcatcaa
     1441 ctttccgttg tagcttcttt tacctgcatc actacccgct gcaccggcaa caacaaaaag
     1501 tggtaccagg tgctcacttc tgggatggct ctcaaaagcg ccaggcgctt cagcccattt
     1561 tacaagggtt tgaattcgtt gttcagggtt tttaacttca acggctgcgg ccagccaact
     1621 gtcaaacttt ttagaggtgg attctacctc cggagagccc gacatgaagc cttgcatgtt
     1681 gtggtagctc atgcccgagc cgatgatgag gactccttca gaacggagtg gctcaagggc
     1741 ttgtccaact gcaatgtggg tggaagggtc aagcccacgg accaacgaaa gctgaacaac
     1801 aggaagttga gcttcaggaa atgcaaccat catgggtaca aatgtaccat ggtcgtatcc
     1861 cctgatcatc tctcgtcggg tgttaaaccc cttgttgtta agaagctgct caaccctcag
     1921 tgccaggtta ggattacccg gtgcaggcca gctaaggtgg taggtgtatt ctggaaaacc
     1981 gtaataatca tagtatagat ttggcgattc gccaaaatgt atggttggaa acgtttcttc
     2041 ccagtgagca gaaaccatca gaatggatcg aatcctgctc ctgtatgaat tccccaattc
     2101 tttaaggtag cctctaaggt ttccgtagcc aacgggatcg cccatggcat cgggtattag
     2161 atgccatgga cctcccccgt gtggaatgaa tatcaccgga agtttatcgc ttccattcat
     2221 aatcctactt tctaatggtt ttaagagcac cgccccaagc caccagctga tcaagcatgg
     2281 ctttgaggtt atcctcatga agtgcagccg gtttgaaaac gctgaagttt tcaaaatcgg
     2341 taaaaagcga cagggccacc tgggtacgca cgtcggccac ctgaagttca cccatgatga
     2401 gacgaagctg ctcaacagcc cgaaccccgc cggttgaacc atagctgaca aaacctgccg
     2461 ccttattatt ccactcaacg tagaggtagt caattgcatt ctttagcgct ccggttgtgg
     2521 catggttata ctccggggtt acaaatataa aagcgtcaaa cgagctgatt ttatctgacc
     2581 acaccttagt gtgatccttc atgtactggt gcatggaggc aggaacaggc tcatcgagaa
     2641 caggaaggtt gaagtcctta atatcaacca gctcaaactc agcgtcggtt ctcctgcggg
     2701 catgttcgta aacccacttg gcaacagcct cggcattcct gccaggtctt gtgcttccaa
     2761 taataattcc aacttttatc attacttcaa gattgtttag tg
//
LOCUS       DAOOQX010000213         2800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_43452_length_2800_cov_3.043716, whole genome shotgun sequence.
ACCESSION   DAOOQX010000213 DAOOQX010000000
VERSION     DAOOQX010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43452_length_2800_cov_3.043716"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="PLX53_04395"
     CDS             <1..1520
                     /locus_tag="PLX53_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72352.1"
                     /translation="EIIHVKTKLTELDFTGIVMGNLQSIEPALDYGLGGTRGAPKFPM
                     PGLLGYLMQSAQATGNLKLKSFVSTTLDNIANGGVYDHVGGGFFRYAVDSRWHTPHFE
                     KMLYDNAQLVELYSLAYRINPNPSYLNIVFNTVDFLDRELHSPDGGFYCSLDADANGL
                     EGGFYTWTKQELDEVLEFDSEIFCSAYGVTPSGDINGRNGLKRCASDDQLSSLFDIPP
                     QEIQFRLQRSLKTLASIRNKRIPPLIDDKVLASWNGLLVSALAQAFITFKNPRFIGLA
                     SEIASYLETKHFVEGNLKRVFCKGKTYGNALLDDYANYIRALIMLYQATLEEKWLFKA
                     NSLAQEAIRDFWDEGKGMFYYTPGSSDLMVRKMELIDGVIPSSSSIMTGNLLFLAKHI
                     ENPLYSKIAMQVLANLAENTKNNGVFLYGWGNLMLSLALPEVSITIFEGTSNDVNDIL
                     GRVIYPNIYFELIAKKVDHKFQLCVGSSCRLPMDSAADVAQAVNGIGLHNNSSNF"
     gene            1939..>2800
                     /locus_tag="PLX53_04400"
     CDS             1939..>2800
                     /locus_tag="PLX53_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72353.1"
                     /translation="MKKIYKGVMVAISVIGLVAILLFIFWQLQPSKKLNIYILDKTVT
                     RSNRPEHRALVWVLNQDRIVGPDGKTYRVNEDYWGFFPIDLNQQLFDFKAIRLNEVDA
                     YAAAYDAAYYADCYGVYSFEWYKNDAQPIRSSKVYGGLNQNDYLLLKSMKDMGKLIIG
                     EYNMFSTPTNALMRSKAEELFNVSWTGWSGVFCRNLTPNEAGGPAPWMPKLYESQHLK
                     PWPSEGKGIILLSNDGLVDVLVDGEDINSAFPVIISDNTGISRFGLISEIPFAGWFEF
                     VVPGSNTVTPS"
BASE COUNT          803 a          602 c          627 g          768 t
ORIGIN      
        1 ccgagataat ccatgtcaag acgaaactga cggagctgga ttttaccgga atagtaatgg
       61 ggaacctgca atccattgaa cctgctttag attacggcct gggtggaacg cgtggtgccc
      121 cgaaattccc gatgccgggg ctgctaggct atctgatgca gagcgcacag gctaccggta
      181 atctaaaact taaaagcttc gtttccacta cgctagataa tattgccaat ggcggagttt
      241 acgaccatgt tggaggaggt ttttttaggt atgccgttga ctccaggtgg cacacacccc
      301 actttgagaa aatgctctac gacaacgctc agcttgttga gctctactcc ctggcctacc
      361 ggataaaccc aaacccatca tacctgaaca tagtgtttaa cacggttgat tttctcgaca
      421 gggagctaca ttcacccgat ggtggttttt actgctcgct tgatgcagat gcgaatggac
      481 tggagggagg cttctacaca tggaccaaac aggaacttga cgaagtcctg gaatttgata
      541 gcgaaatttt ttgctcggca tatggggtaa cgccttcggg agatattaat ggcagaaatg
      601 ggcttaaacg ttgcgcttcg gatgatcagc taagcagctt attcgatatc cctccgcagg
      661 agattcagtt caggctgcag cgcagcctaa aaacccttgc cagcataaga aacaagcgta
      721 ttcctccgct aatagatgat aaagttctag catcatggaa tggtttatta gtttcagccc
      781 tggcgcaggc tttcatcacc tttaaaaatc cacgtttcat tgggcttgcc agcgaaattg
      841 ccagctatct tgaaaccaaa cattttgttg aaggcaacct gaaacgggtt ttctgcaagg
      901 gtaaaaccta tggtaacgcc ctgctcgacg attatgccaa ctacatacgc gccctgatta
      961 tgctttacca ggcaacgctg gaagaaaaat ggttgtttaa agcaaattca ttagcgcagg
     1021 aggccatacg ggatttttgg gatgagggta aaggtatgtt ctattacacc ccgggaagca
     1081 gtgatctgat ggttcgaaaa atggagctga ttgacggggt tattccatct tcatcctcca
     1141 taatgaccgg aaatttgctt tttctggcta agcatattga aaaccccctt tacagcaaaa
     1201 tagccatgca ggtgctggcc aatttggctg aaaatactaa aaacaacgga gtattcttgt
     1261 atggctgggg aaaccttatg ctttcgcttg cacttcctga agttagcata accatattcg
     1321 agggcacatc aaacgatgtt aacgacattt taggcagggt aatataccct aacatttatt
     1381 ttgagttgat cgctaaaaag gttgaccata aatttcagct atgtgtaggt agctcctgca
     1441 ggctccctat ggactcggcc gctgatgtgg ctcaagctgt taatggcatt ggcttgcata
     1501 ataactcatc gaacttctaa gaaataatat ttccgcacca accactcatc caactttttg
     1561 cacttacccg agggttagct gtggcaaaac ctgtataaag gctaatcaca tgcaggcgta
     1621 ttggctcttc gggctgtaaa attaaaagta taagtaaaaa atcgtcttag ccatcaacaa
     1681 tttggccacc gaataattaa tttttcgtaa attggccgta tcgtacttaa accatgcgga
     1741 tttaaacgtt ttggagttta acttttaaaa ataatcaata tacaggcata agctacctaa
     1801 agttaaccat ttatggcgtt tggatgtaag acaaataacc gacagtcaga acgaacagcg
     1861 ctaagtaggc cgctctagca catcagaatt gacaaacatt ttcaccctca accagctaac
     1921 cataagataa ttagatagat gaaaaagatt tataaaggag taatggttgc tattagcgtt
     1981 attgggctag tggcaatctt gctgttcata ttctggcagc ttcagccctc aaaaaaacta
     2041 aatatctata tcctggacaa aacagttacc cggagcaatc ggccagagca tagggccttg
     2101 gtttgggtgc ttaaccagga caggattgtt ggtcccgatg gcaagaccta tcgggttaat
     2161 gaggattact ggggattttt ccccattgat ttgaaccaac agctattcga ctttaaggct
     2221 atacggctaa atgaggttga tgcctatgct gccgcatacg atgcagcata ctacgccgat
     2281 tgttatggtg tttactcttt tgaatggtat aaaaacgatg cccaacctat acgttcctct
     2341 aaagtttatg gtggccttaa ccagaacgac taccttcttc taaagagcat gaaggatatg
     2401 ggaaaactca tcataggaga atataatatg ttcagcactc caaccaacgc actaatgaga
     2461 agtaaagccg aagagctatt caatgtcagc tggacgggtt ggtcgggagt attctgccgc
     2521 aacttaaccc ctaacgaggc tggtggccct gcaccatgga tgcccaagtt gtatgaatca
     2581 caacacctta aaccatggcc atcagaaggt aagggcatta tcttactcag caatgatggg
     2641 ctagttgatg tgctagttga tggtgaagat ataaattctg ccttcccggt aattatttca
     2701 gataatacag gaatatctcg ctttggtttg atttcggaaa ttccattcgc gggttggttt
     2761 gaatttgttg ttcccggtag taacactgtt accccttcaa
//
LOCUS       DAOOQX010000214         2796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_43532_length_2796_cov_2.870485, whole genome shotgun sequence.
ACCESSION   DAOOQX010000214 DAOOQX010000000
VERSION     DAOOQX010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43532_length_2796_cov_2.870485"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PLX53_04405"
     CDS             <1..209
                     /locus_tag="PLX53_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72354.1"
                     /translation="AKEPRIAAMRGITTARTKPIKLYEPVPVEIMTEYVKYELPKPRA
                     ACKFVDAENPKQLIELLQTETKLI"
     gene            235..1200
                     /locus_tag="PLX53_04410"
     CDS             235..1200
                     /locus_tag="PLX53_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOY72355.1"
                     /translation="MSVLVYTENWDGKFKKLSFELVSYATRVAEMLNTSCIALSIGNV
                     DDAELKKLGSYGAQKILSVNSDNLKVFDTQNYTEAIAQVAKNNDAKVVVISNNNVGKA
                     VAPRLSVKLKAALAAGVNGLPLSTNPFTVRKRAFSGGAIAHVVLKTDVKIVTLPQNSV
                     DVTETPVNAEVEKISISLPEPKTVVKDVQKQAGKLLLTDAEIVVSGGRGMKSPDNWGP
                     LVELAEQLGGATACSRPVSDEGWRPSEEHTGQTGKIIAPNLYIAVGISGATQHIAGVS
                     GSKYIVAINNDKSAPIFEVAQYGIVGDAQKVLPKLVEAVREIKGK"
     gene            1486..>2796
                     /locus_tag="PLX53_04415"
     CDS             1486..>2796
                     /locus_tag="PLX53_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOY72356.1"
                     /translation="MTKQVVFALALLVTLVAFGFTITRIIRFFSLTRKGFPVKNIGKR
                     IGIMLKVAFGQTKIFRRPIIGFLHAMVFWGFCLIIFGSIEMVIDGLFGLEKSLSVLGW
                     FHDFIMAAGDIFAPIIAIAIVVFLIRRVFLHIKRFEGIEMKKISHLDPNIALTLILLL
                     MISLMGMNAAYVSWCHSLGKEIHGVYPVSQHLTGLFSSMPVEVIYTYYEVFWWSHILL
                     IFLFANILPYSKHFHVFMSIPNVFLSRLEPLGKLPSMESITREVKLMLDPNAAFAEPP
                     SDTPIERFGVKDAEDVVWKSYFDSLSCTECGRCTSVCPANLTGKRLSPRKVMMDLRAR
                     MKEKGPLMLKNGRDYSDGKSLIRDYISEEELWACTTCNACAQECPININHPTLIVDMR
                     RYLVMEEGSAPGEIKAMFNNIENNGAPWQYSPEDRLNWANNLEIN"
BASE COUNT          806 a          647 c          648 g          695 t
ORIGIN      
        1 tagctaagga accaagaatt gccgcaatgc gcggtattac gacagcacgc actaagccaa
       61 taaaacttta cgagcctgtt cctgtagaaa tcatgaccga atatgttaag tacgagctac
      121 ccaaaccacg cgcagcttgc aagtttgttg atgctgaaaa tccaaaacag ctcattgagc
      181 tgcttcaaac cgaaaccaag ttaatttagc cttcacccat taaacgatta cgatatgtca
      241 gtactagtat atacagaaaa ttgggacgga aaattcaaaa agctaagctt tgagctggta
      301 tcatatgcaa caagggttgc cgaaatgctc aataccagct gcattgccct ttcgattgga
      361 aatgttgatg atgccgagtt gaaaaagctg ggtagctatg gcgcccaaaa gatattgagc
      421 gtaaacagtg acaacctgaa ggtattcgac acacaaaact acaccgaagc tatagcccag
      481 gttgcaaaaa acaacgatgc aaaagtagtt gtaatttcta acaataatgt tggcaaagcc
      541 gttgcgccta gattatccgt aaagttaaaa gctgcgctag cagcaggtgt aaatggccta
      601 ccgctatcaa ccaatccctt taccgttcgt aagagagctt tttcgggtgg tgccattgcc
      661 cacgttgtgt taaaaaccga cgtaaaaata gttaccctac cccaaaactc tgttgatgta
      721 accgaaactc cggttaacgc tgaagtggaa aaaattagca tcagcctacc cgaacctaaa
      781 accgtggtaa aggatgtaca gaagcaagca ggcaaactgc tgctcactga tgccgagatt
      841 gtagtttcgg gcggacgcgg aatgaaatcg cccgataact ggggaccact ggtggagctg
      901 gccgaacagc ttggaggtgc taccgcatgc tcgcgtccgg tttcagatga aggttggcgt
      961 cccagcgagg agcacaccgg gcaaaccgga aaaataatcg ccccaaacct ttacattgca
     1021 gttggtatct cgggagcaac ccagcatatt gccggtgtga gcggttcaaa gtatattgta
     1081 gctattaata acgataagag tgctccaata tttgaggtag cccagtacgg cattgtaggc
     1141 gacgcccaaa aggttcttcc caaactggta gaagctgtta gggaaattaa aggaaaatag
     1201 ccaatgccga tgtttggcag ctccaatatt ggtgttttgg ctccacgagc agaaaaaatt
     1261 gcagcctaaa aaaacaacat gcaggagata aacaagcagt gctcctgcat gtaaggttat
     1321 gtttaatgaa tagattcagg tgattaaatc cacactacgc agcaggagga tgtccggttg
     1381 cacgaagttc cataaaatag cccatgatat gaacataggc ggcatacacc actgcattac
     1441 ctgcaaaccc gacgaaacaa tagaataata aaaaactgaa gctccatgac caaacaagtt
     1501 gtttttgcat tagcattgct ggtaacgctg gttgcttttg gttttaccat aaccagaatc
     1561 atccggtttt tctcgttaac ccgtaagggg tttccggtta aaaatattgg aaaacgtatt
     1621 gggataatgc tcaaggtggc atttggccaa accaaaattt ttcgtcggcc catcattggg
     1681 tttctacacg ccatggtttt ctggggattc tgcttaatca tctttggaag catcgaaatg
     1741 gtcattgacg ggctgttcgg gcttgaaaag tcgctgagcg ttttaggttg gtttcacgac
     1801 ttcatcatgg ctgccggcga tatctttgca cctatcatag ccattgccat tgtggttttt
     1861 ttaatacgca gggtattcct gcatattaaa cgcttcgagg gcatcgagat gaagaaaatt
     1921 tcgcacttag atcccaacat cgcccttacg ctaattttgc ttttaatgat ttcccttatg
     1981 gggatgaacg ctgcctacgt gagctggtgc cattctcttg gaaaggagat tcatggagtt
     2041 tatccggtaa gccagcacct taccggtctg ttttcctcca tgccggtaga ggtcatttac
     2101 acttactacg aagtcttttg gtggtcacac attctgctca tttttctttt tgccaacata
     2161 ctcccctact ccaagcactt tcatgtgttc atgtccatcc ctaacgtatt tctgtcgagg
     2221 ctggaaccac tgggcaagct acccagcatg gagagcatta cccgcgaggt taagcttatg
     2281 ctggatccga acgctgcctt tgccgaacct ccttccgaca caccaatcga aagatttggc
     2341 gtaaaggatg ccgaggatgt cgtttggaaa agctacttcg attccctctc ctgcaccgaa
     2401 tgcggacgct gcacctccgt ttgcccggca aacctaaccg gcaaaaggct ttcaccccgc
     2461 aaggtaatga tggacctgcg cgcaaggatg aaggagaaag gcccattaat gctgaaaaat
     2521 ggaagggatt acagcgatgg caaatcgctg attagggact acatctccga ggaggagctc
     2581 tgggcttgta ctacctgcaa cgcctgcgcc caggagtgtc caatcaacat taaccatccc
     2641 accttgattg tggatatgcg ccgctatctg gtcatggagg agggctcagc acccggagaa
     2701 ataaaggcaa tgtttaacaa tatcgagaac aacggagcac cttggcagta ctcaccagaa
     2761 gacaggctta actgggctaa caatttggaa atcaac
//
LOCUS       DAOOQX010000215         2794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_43565_length_2794_cov_3.322746, whole genome shotgun sequence.
ACCESSION   DAOOQX010000215 DAOOQX010000000
VERSION     DAOOQX010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43565_length_2794_cov_3.322746"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..490)
                     /locus_tag="PLX53_04420"
     CDS             complement(110..490)
                     /locus_tag="PLX53_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOY72357.1"
                     /translation="MTDQLDNQNEFDRAEIEDLFSNVVKAGKRTYFFDVKATRSDEYY
                     LTITESKKRMGKDGNMFYEKHKIFLYKEDFDNFMGGLTEAVNFIKSNQEVLPRIAAEY
                     GNEASLAEVNDELTTLNFEDLDTK"
     gene            714..1709
                     /gene="nusB"
                     /locus_tag="PLX53_04425"
     CDS             714..1709
                     /gene="nusB"
                     /locus_tag="PLX53_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOY72358.1"
                     /translation="MINRRLLRIKILQVLFAYFRSNNDSLSNLEKELHFSIEKSYQLY
                     LLVLLLPEALADVALEKIELGKQKFRPTADELNPNTRFVDNPLIHILRNNQALKQKAK
                     DSKLSWKNYPEVVKLLYQQLVSSESYHKFMSDDEFSLDTCRQMIVNFYLNELENSDEF
                     DAFLEEQSIYWNDDLKFVLSMAAKTIKKVREENPDALKLMPLYRNEEDKDFAFQLMAK
                     AVLKHSDNVGLIEEYTQNWEVDRIASMDMLIMEMAITEVLEFPSIPVKVSLNEYIEIA
                     KFYSTEQSGLFINGVLDKIITTLRQQKKFEKQGRGLIGDIAETPDDINSLEPDTE"
     gene            1831..2250
                     /locus_tag="PLX53_04430"
     CDS             1831..2250
                     /locus_tag="PLX53_04430"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOY72359.1"
                     /translation="MRTRGMVVMCVLLLTTCGSPSKPKNNLSTSGNSSGVPNLEYIED
                     FFDFGTITQGEVVTHTFRFKNSGNAPLVVKDVIPGCGCTTSKLSSTILKPGEEGTVEV
                     IFNSQGWAGLQYKTITLRTNSSIMDKSVTIRANVVMP"
     gene            2317..2634
                     /gene="yajC"
                     /locus_tag="PLX53_04435"
     CDS             2317..2634
                     /gene="yajC"
                     /locus_tag="PLX53_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOY72360.1"
                     /translation="METLFVLLQKPAAQGANPLMTILMFALIIVVFYFFMIRPQMKRQ
                     KELRKFQEALKKGDKVIIAGGVYGKISEVKDDYVMVEISDNINVKVAKSTIIRDVSDI
                     QAK"
BASE COUNT          859 a          563 c          543 g          829 t
ORIGIN      
        1 ttttgatctg tatatataac cggattcacc tactaaactt aaatttatat cggaaaacta
       61 taacaaaaaa gccggcaaat agccggcttg catgctctag tttaattatc tactttgtat
      121 ctaagtcctc aaagttcaac gtagtaagct catcgtttac ctcggccagt gaagcttcgt
      181 tgccatactc tgccgctatg cggggaagca cttcctggtt cgacttgatg aagttgactg
      241 cttcagtcaa accacccata aagttatcaa aatcttcttt gtacaggaat attttatgct
      301 tttcgtagaa catattgcca tccttgccca tccttttctt gctttcggta atggtaaggt
      361 agtactcatc gctacgggtc gcttttacat caaaaaagta ggtacgcttg ccagctttta
      421 ccacatttga aaaaaggtct tcaatttctg ccctgtcaaa ttcattttga ttatcaagtt
      481 gatcggtcat cactgtaaga gttttagttt aaggataaac aatgctttta tgaccatcaa
      541 aaataaaaaa ttaatcgtct atccaacctt ttgattttaa aaaattattt accagaatgt
      601 tttggttttg ttttatattt tattaaccgc aagattattt tatatctaat atagctactc
      661 atgctatttt gttttaactt tgtaggcttt ttactttgaa aatttgttct taaatgatta
      721 accgacgctt actccggata aaaattctac aagttctttt tgcgtatttc cgaagcaata
      781 acgattcgct tagcaacctt gaaaaggagc tccattttag cattgaaaag tcctatcaac
      841 tttacctttt agtgctatta cttcctgaag cactcgccga tgttgccctg gagaaaattg
      901 agctgggtaa acaaaagttc cgtcctaccg ccgatgagct aaatccaaat acccgttttg
      961 ttgacaatcc gctaattcat attctcagaa ataatcaagc gctcaagcag aaagcaaagg
     1021 attccaagtt gagctggaaa aactaccccg aggtggttaa acttctctac cagcagctgg
     1081 tgtcctccga aagctaccat aaattcatgt cggacgacga attttccctc gatacctgca
     1141 ggcagatgat tgttaacttc tacctaaacg agcttgaaaa ttctgatgaa tttgatgctt
     1201 ttctcgagga gcagagcatc tactggaacg atgacctgaa gtttgtgctt agcatggctg
     1261 ctaagaccat taaaaaggta agggaagaaa atcctgatgc tttaaagttg atgcctttat
     1321 accgaaacga ggaggataag gactttgctt ttcagctaat ggcaaaagcg gtactcaagc
     1381 attctgataa tgttggcctc attgaggagt acacccagaa ctgggaggtt gaccggattg
     1441 cgtcaatgga tatgctgatc atggaaatgg ctattaccga ggttcttgaa tttccatcaa
     1501 ttccggttaa ggtaagccta aatgagtaca tcgaaattgc aaagttctac agcacggagc
     1561 aaagcggact ttttataaac ggtgtactcg ataaaataat taccacactc cgccagcaaa
     1621 agaaattcga gaagcagggg agaggactta ttggcgatat agccgaaact cctgatgaca
     1681 taaacagctt agaaccagat accgaatagc caggctaaat tcagcaacca ccaacatccg
     1741 caccctgaag tagcaatttc agctgttttc agttgcaaat tcaaaaggtt tatagaacaa
     1801 gttcagctaa aaattactaa atattctggt atgagaacaa gaggaatggt tgttatgtgt
     1861 gtactgctgc ttaccacctg tggctcgccc tccaaaccta aaaataatct gagcacctct
     1921 ggtaacagct caggggttcc aaacctggag tatatcgaag atttttttga tttcggcaca
     1981 attactcagg gagaagtggt aacccacaca ttccgtttta agaattccgg aaatgccccg
     2041 ttggtggtga aggatgtaat tcctggttgc ggatgcacta cctctaagct aagcagcacc
     2101 attttgaaac ccggtgagga gggaaccgtt gaagttattt tcaacagcca gggatgggcc
     2161 ggattgcagt ataaaacgat tacccttcgg acaaattcat ccattatgga caaatcggtt
     2221 actatcagag caaacgtcgt gatgccatag gttgcttcat aaaaaaatac tacctttgct
     2281 tcgaaattta atgcttaaca aactaaacac ttaaagatgg aaacactatt cgtactattg
     2341 caaaaaccag ctgcacaagg ggctaacccc ttaatgacta ttttaatgtt tgcactgatc
     2401 atcgtggtat tttacttttt tatgatccgt ccgcaaatga agcgccaaaa agaacttcgc
     2461 aagtttcagg aagccctgaa aaagggggat aaggtgatta ttgccggagg tgtttacggt
     2521 aaaatcagcg aggttaagga tgattacgtt atggttgaaa tttctgataa catcaacgtt
     2581 aaggttgcta aaagcacaat aattcgtgat gtttccgata ttcaagccaa atagctataa
     2641 ccttgatgaa agcattgcag gattcagcct tgggcaacga atcctgcatt tacttcctta
     2701 aagttggatt caaccctact caaacagttc aacaagataa tatatcgcga aaaggctaat
     2761 tttaaaaagc gcttggcggt attttccctt tttt
//
LOCUS       DAOOQX010000216         2792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_43609_length_2792_cov_3.739861, whole genome shotgun sequence.
ACCESSION   DAOOQX010000216 DAOOQX010000000
VERSION     DAOOQX010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43609_length_2792_cov_3.739861"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2432
                     /gene="leuS"
                     /locus_tag="PLX53_04440"
     CDS             <1..2432
                     /gene="leuS"
                     /locus_tag="PLX53_04440"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOY72361.1"
                     /translation="DWSREFRTCEPSYYKWTQWAFIQMFKHWYNNKTQKAEPIDELVA
                     EFERNGNQHVDAACSETKIFTASDWRNMDGRQQQELLLSYRLAYLADVMVNWCPALGT
                     VLANDEVKEGVSIRGGHPVEQRKMRQWCLRISAYAERLLSDLEQLDWTDSLKEIQRNW
                     IGRSEGAEVLFSVADSRQQIKIFTTRPDTIYGATFMVLAPESELLDGLTKPEYADNVE
                     AYCNEVKRKTERERVAEAKKITGQFTGSYAVNPFTNQHIPIWVSDYVLAGYGTGAIMA
                     VPAHDSRDFAFARTFNLPIIQTVVRKGEQPTNPETWEESYDAKDGIVVNSPLIDGLEV
                     KQAITRICEIIEQRGLGKSKVNYRLRDAIFSRQRYWGEPFPIYYKDGLPYPLPEEKLP
                     LELPDVDAFLPTEKGEPPLGRAKNWHTPEGYPYELSTMPGFAGSSAYYLRFMDPHNDS
                     QLVSKEANTYWENVDLYIGGTEHAVGHLIYSRFWNKFLYDLGYVCKSEPFKKLINQGM
                     IQGRSSFVYRIKGTNTFVSLNLKGSYEVTPIHVDVNIVNNDVLDVEAFKKWRPEFSNA
                     EFILEDGKYICGWAVEKMSKSMWNVVNPDTIVEKYGADTLRMYEMFLGPLEQSKPWDT
                     NGIDGVHKFLKKFWRLFHNPANQLEISDDNPTPQELKVLHQTIKKVSDDIERFSFNTG
                     VSAFMICVNELTDLKCNKRQVLEPLLILISPYTPHIAEELWSQMGYTGSICDASWPLF
                     NEELVRESTFTCPISFNGKMRFTLDIPLNLPVAEVEKLVLADSNTQRQLDGKTPKKVV
                     VVPHKIVNIVV"
     gene            2532..>2792
                     /locus_tag="PLX53_04445"
     CDS             2532..>2792
                     /locus_tag="PLX53_04445"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOY72362.1"
                     /translation="MEKSENKTKSFRDLIVWQKAHALVLNIFQQVKTFPEDQQQMVGD
                     YLCESAMAIATNIVGGYKKKDRDEKLLYLSAAQDALEECRYYI"
BASE COUNT          766 a          640 c          690 g          696 t
ORIGIN      
        1 acgactggtc aagggagttt cgcacctgcg agccatcata ctacaaatgg acacagtggg
       61 catttattca aatgtttaag cactggtaca acaacaaaac acagaaggcc gaaccaattg
      121 atgagctggt agccgagttt gagcgaaacg gcaatcagca tgttgatgct gcttgtagcg
      181 aaaccaaaat ttttactgct tcagattgga ggaatatgga tggcaggcag cagcaggaac
      241 ttcttctgtc ttaccgactg gcataccttg ccgatgttat ggtaaactgg tgcccagcac
      301 tgggaaccgt tctggccaac gatgaggtga aggagggcgt ttctatccga ggtggtcacc
      361 cggttgagca gcgtaagatg aggcaatggt gccttcgcat ttcggcttac gccgaacggc
      421 tgctttccga tttagagcag ctggactgga cagactcgct taaggaaata cagcgaaact
      481 ggattggccg ctcggaaggg gcagaagtgc ttttcagcgt ggccgatagc cggcagcaaa
      541 ttaaaatttt taccacccgt cccgacacca tctatggggc aaccttcatg gtgcttgctc
      601 cagagtcgga gctgctggat ggtcttacca aacccgagta tgccgataat gttgaggcat
      661 actgcaacga ggtgaaacgc aaaactgaac gtgagcgggt ggccgaggcc aaaaaaatta
      721 ccggccagtt caccggaagc tatgcggtga atccctttac caaccagcac attcccattt
      781 gggttagcga ctacgtgctg gcgggatatg gaacaggcgc catcatggct gttcctgccc
      841 atgacagccg cgacttcgcc tttgcccgaa ctttcaacct ccctattatt caaacagttg
      901 ttaggaaggg cgaacaacct accaatccgg aaacatggga ggaatcatac gacgcgaagg
      961 atggcatcgt ggtgaacagc cccctgattg atgggcttga agtcaaacaa gccattacac
     1021 gaatatgcga aatcattgag cagcgggggt tgggtaaaag caaggtgaac taccgcttgc
     1081 gcgatgccat ttttagccgt caacgttact ggggcgagcc tttcccaatt tactataagg
     1141 atggattacc ttaccccttg cccgaagaga agttacccct tgaacttccg gatgttgatg
     1201 cttttttgcc aaccgaaaaa ggtgaaccac ctttaggaag agcaaagaat tggcatactc
     1261 ccgaggggta cccctacgag ctgagcacca tgcctggatt tgccggttcg tcggcctact
     1321 accttcgctt tatggacccg cacaacgata gccagctggt gtcgaaagag gcaaacacct
     1381 actgggaaaa tgttgacctt tacattgggg gcaccgagca tgctgttggc caccttatat
     1441 actcccgctt ttggaataag ttcctctatg accttggcta cgtttgcaaa agcgaaccct
     1501 ttaaaaaact aatcaaccag gggatgatac aggggcgctc cagctttgtt taccggatta
     1561 agggtactaa cacctttgtt tcccttaacc tgaagggaag ctacgaggtt acccccatcc
     1621 atgttgatgt gaatattgtg aacaacgatg ttcttgatgt tgaggcattt aaaaaatgga
     1681 ggcctgaatt tagcaacgcc gagtttatcc tcgaggatgg gaaatacatt tgcggctggg
     1741 cggttgagaa gatgagcaaa agcatgtgga acgtggtaaa tcccgataca attgttgaaa
     1801 agtacggtgc agatacccta cgcatgtacg aaatgttttt gggccccctg gaacaaagca
     1861 aaccatggga taccaatggt atagacgggg tgcataaatt ccttaaaaag ttctggcggt
     1921 tattccataa cccagccaac cagcttgaga ttagcgatga caatcctaca ccgcaggagt
     1981 tgaaggtgct gcatcaaacc atcaaaaagg tttccgacga cattgagcgc ttcagcttca
     2041 atacgggtgt ttcggccttc atgatttgtg tgaatgaact caccgacctt aagtgtaata
     2101 aacggcaggt gctggagcct ttactcattc taatttcacc ctataccccc cacattgccg
     2161 aggaactgtg gagccagatg ggttataccg gttccatttg cgatgcaagc tggccattgt
     2221 ttaacgagga gcttgtgagg gaaagcacct ttacctgccc catttcattc aacggaaaaa
     2281 tgcgttttac tcttgacatc cctctgaatt tgccggtggc cgaagttgaa aagctggtat
     2341 tggctgacag caacacccag cgacagcttg atggcaaaac ccctaaaaaa gtggtggtgg
     2401 tgccccataa aattgttaat attgttgtgt agaagttgtt gtatgttatt ttttaagtat
     2461 cttcgtattc acccgtttag ctatagcgat atagcaacct ttcaaattct aattttctaa
     2521 agattaacaa gatggaaaaa tctgaaaaca aaaccaaatc gttccgtgat ctaattgtat
     2581 ggcaaaaggc ccacgctctg gtgttgaata tttttcagca ggttaaaact tttcctgagg
     2641 atcagcagca aatggtaggc gattaccttt gcgaaagcgc catggctatt gctactaata
     2701 ttgtaggtgg ctataaaaag aaggacaggg atgaaaaact cctatacctt tcagctgccc
     2761 aggatgctct tgaagagtgt cgctactaca tt
//
LOCUS       DAOOQX010000217         2779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_43896_length_2779_cov_3.219163, whole genome shotgun sequence.
ACCESSION   DAOOQX010000217 DAOOQX010000000
VERSION     DAOOQX010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43896_length_2779_cov_3.219163"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1521)
                     /locus_tag="PLX53_04450"
     CDS             complement(133..1521)
                     /locus_tag="PLX53_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOY72363.1"
                     /translation="MISTNIQIAAYLFASILFILSLSGLSSQESAKKGVIYGIVGMAI
                     ALCATLFGGNIQGYVYIIVAMVVASVIGVIVARKVQMTAMPQLIALLHSFVGLAAVLV
                     GFSSFIDPKTVSMAGAERAIHMVEIFVGIFIGAITFTGSIIAWGKLTENITSKPLVFK
                     GKDVVNVVLLLASVVLGVMFVGQGGTHGMPFLIAMTVIAAFFGVMLVMAIGGADMPVV
                     ISMLNSLSGWTAAASGFMLGNDLLIVTGALVGSSGAILSIIMCRAMNRSFFAVISGGF
                     GQEVKTGRKLEGKVNSMTHDEAAEWLKEAQSVVIVPGYGMAVAKAQYPIHDMVEKLRS
                     QGKSVKFGIHPVAGRLPGHMNVLLAEANVPYDIVLEMDEVNPELPETDVAMVVGANDT
                     VNPGAQEDPSSPIYGMPVVEVWKAKRVIVMKRSMAVGYAGVENPLFYKENVSMLYGDA
                     KDSVDKINAKLG"
     gene            complement(1529..>2779)
                     /locus_tag="PLX53_04455"
     CDS             complement(1529..>2779)
                     /locus_tag="PLX53_04455"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HOY72364.1"
                     /translation="YSINTCLGKLAEAGINAIAMDAIPRISRAQKMDVLSSMANISGY
                     RAVVEAANYFGRFLNGQITAAGKVEPAKVLVIGTGVAGLAAIGTAKSLGAIVRAFDTR
                     KEAREQIQSMGAQFITVEIDEDGSTASGYSKEMSQAYIDAEMALFKRQAAEVDIIITT
                     AQIPGKAAPKLLLEEHVMAMKPGSVIIDLAAPTGGNCVLTKKDEVYTTPNGVVIAGRV
                     DTLPTQASFLYGNNLCNLLDDMGKAENFRIDMNDDVIKRAMVTYNGKVNWPPEPLAVS
                     AQAKKTEAGKPSPEEEAAKKAGKVKKALISNVIWLAVVGALLFLLGKVAPADFMGHIT
                     AFVLAVFVGWNVIWNVTHALHTPLMSVTNAISGIIIIGGLLQIQNDFSDPRTIMAFIA
                     ILIAFINIVGGFTVTYRMLKMFKK"
BASE COUNT          743 a          754 c          586 g          696 t
ORIGIN      
        1 tccttcagga gcctgacaag ttccgccggt aggcggacag gtctttgaat tttgaattct
       61 ttatccttct cacatctgca aaatagatac aaaaaatccc ggtttcccgg gatttttact
      121 tgctggtccg aactagccaa gttttgcatt gattttatca acgctatcct ttgcatcgcc
      181 gtaaagcatg ctcacgtttt ccttgtagaa aagtgggttt tccaccccgg catacccaac
      241 agccatggaa cgcttcataa caatcactct tttggctttc caaacctcca ccacgggcat
      301 tccgtaaatc gggcttgatg ggtcttcctg agctccgggg ttcaccgtat cgttagctcc
      361 aaccaccatg gctacatctg tttcgggaag ttccgggtta acctcgtcca tctccagcac
      421 gatatcgtag ggcacgttgg cctcggcaag aagaacgttc atgtgacccg gaagccttcc
      481 ggcaaccggg tgaatgccaa atttaaccga tttgccctga cttcgaagtt tttccaccat
      541 gtcgtgtata ggatattgtg cctttgccac agccatgccg tatccgggaa caattacaac
      601 agattgagcc tcttttagcc actctgccgc ctcatcatgc gtcatcgagt taaccttacc
      661 ctccagcttt ctaccggttt ttacctcctg gccaaagcct ccggaaataa ccgcaaaaaa
      721 cgacctgttc atggccctgc acatgataat ggaaaggata gcccccgagc tgcccaccag
      781 cgcccctgta actatcagca aatcgttgcc cagcataaaa cccgaagcag cagcggtcca
      841 tcccgaaagc gagttgagca tagaaattac taccggcatg tcagcacctc caattgccat
      901 aaccagcatt actccaaaaa atgctgcaat aacggtcatg gcaataagga acggcatacc
      961 atgagtgcca ccctggccaa caaacataac gcccaagact actgaagcca acaaaagaac
     1021 tacattaacc acatcctttc ctttaaatac aagaggttta ctggtaatat tttccgttaa
     1081 cttcccccag gcaataattg aaccggtgaa ggtgatggcc ccgataaata ttcccacaaa
     1141 aatttccacc atgtggatag ccctctcggc gccagccatg ctaacggttt tggggtctat
     1201 gaatgagcta aaacccacca gaacggcagc tagtccaaca aaactatgca aaagggcaat
     1261 cagctggggc atggccgtca tctgcacctt gcgggcaaca ataacgccaa taaccgaagc
     1321 aactaccatg gcaacaataa tgtaaacgta gccttgaata ttaccgccga acagggtggc
     1381 acacagggca attgccatgc ccacaatgcc ataaattaca ccctttttgg ccgattcctg
     1441 tgacgataac cccgacaagc ttagaatgaa aagaattgag gcgaaaaggt aggctgctat
     1501 ttgaatattg gttgaaatca tactccgttt actttttaaa cattttcaac atcctatagg
     1561 taaccgtaaa accgccaaca atgttgatga aggcaatcag tatggctatg aatgccataa
     1621 tggttctagg gtcagaaaaa tcgttctgta tttgcagcaa accgcctatg ataatgatac
     1681 cactaatggc gttggtaacc gacatcaacg gggtatgcag ggcatgggta acgttccata
     1741 taacattcca gccaacaaaa acagccagaa caaaagcggt gatatgcccc atgaaatcag
     1801 caggtgcaac ttttcccagg aggaataata aggctccgac aacggcaagc catatgacgt
     1861 tggaaatcag cgctttttta acttttccgg cctttttggc agcttcctcc tcaggggaag
     1921 gcttaccagc ttcggtcttt ttggcttgtg cgctaaccgc cagcggctca ggaggccagt
     1981 ttaccttgcc attgtaagta accattgccc gcttgatgac gtcgtcgttc atgtctatcc
     2041 tgaaattttc cgcttttccc atatcgtcga gcaggttaca caggttgttg ccatatagga
     2101 agctggcctg ggtgggaagg gtatcaaccc ttccggcaat caccacaccg tttggagttg
     2161 tgtaaacctc atcctttttg gttagcacgc agttaccacc tgtaggagcc gccagatcaa
     2221 taataaccga gcccggcttc atagccataa catgttcctc aagcagcagc tttggagcgg
     2281 ccttccctgg tatttgcgca gtagtgatga tgatatcaac ttcagcagct tgcctcttaa
     2341 acagcgccat ttcggcatca atataagcct ggctcatctc cttgctatag cccgaagcag
     2401 tggagccatc ctcgtcaatc tcaaccgtta tgaattgtgc tcccatggac tgaatttgct
     2461 ctctggcctc cttacgcgta tcgaatgccc taacaattgc tccaagcgat tttgccgtgc
     2521 caatagctgc caggcccgca actccagttc cgataaccag cactttggca ggctcaacct
     2581 tccctgcagc ggtaatctgg ccgtttaaaa accgtccaaa atagttagcg gcttcaacta
     2641 cagctctgta tcccgaaatg ttggccatag acgatagcac atccattttc tgtgccctcg
     2701 aaattctagg gatggcatcc atggctatag cattaatacc tgcctcggca agcttgccga
     2761 ggcaggtatt aatgctata
//
LOCUS       DAOOQX010000218         2774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_44032_length_2774_cov_2.686650, whole genome shotgun sequence.
ACCESSION   DAOOQX010000218 DAOOQX010000000
VERSION     DAOOQX010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44032_length_2774_cov_2.686650"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PLX53_04460"
     CDS             <1..221
                     /locus_tag="PLX53_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72365.1"
                     /translation="DKQKKCNEFINAVSDSIPLIPTLGDADTILMPVEPVWGDKYPFP
                     GMIRLSLGIENGQEITRVISQGLDAVGS"
     gene            complement(315..2057)
                     /locus_tag="PLX53_04465"
     CDS             complement(315..2057)
                     /locus_tag="PLX53_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOY72366.1"
                     /translation="MMNSVFLPTSLKEINALGWESADIILFTGDAYVDHPAFGAAVIG
                     RVLENEGYKVAIVPQPNWRDDLRDFRKLGRPKLFFAVTSGNMDSMVNHYTANLRLRST
                     DAYSAGGKAGFRPDYAITTYCNILKKLYPEVPIVIGGIEASLRRLTHYDYWSDTLKPS
                     ILIDSHADILVYGMGEQPIVEIARRIANGKASSELKNIPQTAFFVANIPEVAADSSLI
                     ELHSYEDCIKSKKYFAENFKLFERESNKLVQKTIVEPVGDGFVVVNPPYPPPAVGEID
                     KWYALPYTRLPHPRYKGKDIPAWEMIKFSVNIHRGCFGGCSFCTISAHQGKFIASRSE
                     KSVLREVEQVIEMPGFKGFLSDIGGPSANMYMMGGIKREKCRECTRYSCIYPSICLNL
                     NASHSAANNLYIKIRSMHGVKKAFVSSGVRYDLFTGRNTQAEKDYFENLCRFHISGRL
                     KVAPEHTSEKVLKLMRKPSFRKFRQFKTEFDLINKKYALNQQLIPYFISSHPGSTEAD
                     MQELAREIKELNIRPEQVQDFTPTPMTLASAMYYTGINPYTGEKIYVARSRKEKLKQN
                     SYFFWYKAQNTKGS"
     gene            complement(2061..>2774)
                     /locus_tag="PLX53_04470"
     CDS             complement(2061..>2774)
                     /locus_tag="PLX53_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOY72367.1"
                     /translation="CGEVPIPPYLNRQSEEVDKYRYQTIYAQPEGSVAAPTAGLHFTP
                     EVFSNLKKLGITPDYITLHVGAGTFKPVKADVIINHEMHTEHFLADIDLIKGMSECDN
                     FRVVVGTTTLRTLESLYWLGVKASQNQLCPPYFISQWEPYKTEPIPFKAAFYELWRHM
                     EANGLETLMASTQIIIAPGYNVQTADALVTNFHQPRSTLLLLVSAFIGEQWRDVYAYA
                     LRNDFRFLSYGDSSLLFRS"
BASE COUNT          702 a          628 c          611 g          833 t
ORIGIN      
        1 aagataagca gaaaaaatgc aatgagttta ttaatgccgt ttcggatagc atacccctaa
       61 tccccaccct gggggatgct gatactatcc tcatgcctgt tgagcctgtt tggggcgata
      121 aatacccatt ccccggtatg attaggttat cgttgggcat tgaaaacggt caagaaatca
      181 cccgggtgat ttcgcaaggg ctcgatgcag ttggaagttg attcccctgg tatgggctaa
      241 tgaccaacct tttggtgagg ttacatcaac ctttgtcagg ataatttttt aagactgacc
      301 accctgtttg tgtattagct tccttttgtg ttttgggctt tataccagaa gaagtagcta
      361 ttctgcttga gcttttcctt tcggcttcgg gccacataaa ttttttcacc agtataggga
      421 tttattcctg tgtagtacat tgccgaagct agggtcattg gcgttggagt gaaatcttgc
      481 acttgctcgg gtcttatatt tagctcctta atctccctgg ctaactcctg catgtctgct
      541 tcggtacttc ccggatggct cgaaataaag tagggtataa gctgttgatt taaagcatat
      601 tttttattta taaggtcaaa ttcggtctta aattgccgaa acttcctgaa gctgggtttt
      661 ctcatcagtt taagaacttt ttcggacgtg tgctccgggg ctacctttaa cctacccgag
      721 atgtgaaaac gacaaaggtt ttcgaaataa tccttttcgg cctgggtgtt tctgcctgta
      781 aacaaatcat accttacccc gctgctaaca aacgccttct taaccccatg catgctgcga
      841 attttaatgt acaggttgtt tgctgcgcta tggctggcat ttaggtttaa gcaaatggaa
      901 ggatagatgc aggagtaacg agtgcactcc ctgcattttt ctcgctttat tccacccatc
      961 atgtacatat ttgccgaagg ccctccaata tcgctaagga atcccttaaa gccaggcatt
     1021 tcaattacct gctctacctc tcgtagtaca gatttttccg accttgaagc aatgaattta
     1081 ccttgatgag ctgatatggt gcaaaagctg caacccccaa agcacccgcg atgaatgttg
     1141 accgaaaatt ttatcatttc ccatgccgga atatcttttc ccttatacct gggatgtggt
     1201 aatcgggtgt agggtaatgc ataccacttg tctatttcac ctacagcagg cggtggatag
     1261 ggtgggttta ccacaacaaa accatcacca acaggctcta ctatcgtttt ctgaacaagc
     1321 ttgttggatt ccctttcaaa aagtttaaaa ttttcggcaa aatatttttt actctttatg
     1381 caatcttcat aggaatgtag ttcgatgagg ctactatctg cagcaacttc tggaatatta
     1441 gcgacgaaaa aagcggtttg gggtatattc tttagttcgg atgatgcttt accattagct
     1501 atccgtcggg ctatttccac aattggctgc tcacccattc cgtacactag tatatcggca
     1561 tgactatcaa tcaaaatgct gggtttcagc gtgtccgacc agtaatcgta gtgagtcagt
     1621 cgccttaaag atgcttcaat tcccccaatc acgataggca cttcagggta gagtttctta
     1681 agtatgttgc agtaggttgt tatggcgtag tcggggcgaa accctgcttt ccctcctgct
     1741 gagtatgcat ctgtgctcct aagcctcagg ttggccgtat agtggttaac catcgaatcc
     1801 atattgcccg aggtaacggc aaaaaaaagt tttggcctgc caagcttgcg gaaatccctt
     1861 aaatcgtctc tccagtttgg ctgtggcaca atggccacct tgtatccctc gttctccagc
     1921 acccgcccaa taaccgctgc cccaaaagca ggatggtcaa cgtaggcatc tcctgtaaat
     1981 aaaattatat cagcgctctc ccaacccaga gcatttatct cctttaggct tgtaggtaga
     2041 aatacgctgt tcatcatatt ttatgacctg aaaagtaagc tggagtctcc atagctaaga
     2101 aaacgaaaat cattcctgag cgcataggca taaacatcac gccattgctc accaataaat
     2161 gccgatacca gcagtaaaag agtcgaacgg ggctggtgaa agttggtaac aagcgcgtct
     2221 gccgtttgaa cgttatatcc tggtgctata attatttgtg tcgaggccat cagggtctct
     2281 aggccattag cctccatgtg gcgccaaagt tcataaaaag ctgctttgaa gggaataggt
     2341 tcagttttat agggttccca ttgcgaaatg aagtatggag ggcatagctg gttttgggag
     2401 gcctttacgc caagccaata aagactttca agtgttctaa gggttgttgt tcccaccacc
     2461 actctaaaat tgtcgcattc ggacataccc tttatgaggt caatatcggc aaggaaatgc
     2521 tcggtgtgca tttcgtggtt gattattaca tcggccttaa ctggcttaaa tgtccctgca
     2581 ccaacgtgaa gcgttatgta atcaggagta attcctaact tttttaaatt gctaaatacc
     2641 tcgggagtga agtgcaaccc tgctgttggt gctgccactg acccttcggg ttgggcatat
     2701 atggtctgat accggtactt gtcaacttct tccgattgcc tattcaggta ggggggtatg
     2761 ggcacttcac caca
//
LOCUS       DAOOQX010000219         2771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_44073_length_2771_cov_3.828424, whole genome shotgun sequence.
ACCESSION   DAOOQX010000219 DAOOQX010000000
VERSION     DAOOQX010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44073_length_2771_cov_3.828424"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            696..947
                     /locus_tag="PLX53_04475"
     CDS             696..947
                     /locus_tag="PLX53_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOY72368.1"
                     /translation="MLAANFTEFRVGLKKFLDEVEMNNETLIIKRGAGMGAVMMSLEE
                     YNSIMETLHLLSSKANAERLFESINQMNERKVVKGKLLE"
     gene            947..1210
                     /locus_tag="PLX53_04480"
     CDS             947..1210
                     /locus_tag="PLX53_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOY72369.1"
                     /translation="MTRITFSRNSWEEYKSWQVDDRLILKKINELIKDIERHPYEGLG
                     KPEPLRFDLAGFWSRRIDREHRLVYRVIDQEILIYSCRYHYDK"
     gene            complement(1661..2374)
                     /locus_tag="PLX53_04485"
     CDS             complement(1661..2374)
                     /locus_tag="PLX53_04485"
                     /inference="COORDINATES: protein motif:HMM:NF017755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOY72370.1"
                     /translation="MANSYFRFKQFKVFQEKSAMRVNTDGVLLGAWADVSGVQRILDV
                     GTGTGVIALMVAQRSPNAMVDAVEIDPPSAEEAAGNAARSPWANRIRVVCTSFQDFAS
                     CAEFRYDLIVSNPPFFNHSLKSPDSKRTLSRHTDTLPTPALLEGATKLMLPGGRFCGI
                     FPYAEGNVFIAKASHYGLFCSKKLNILSKPGGRTLRMLVQLEAERMATAESSLCIHTA
                     DGLFSKEYRSLTSEFYIAF"
     gene            complement(2394..2663)
                     /locus_tag="PLX53_04490"
                     /pseudo
     CDS             complement(2394..2663)
                     /locus_tag="PLX53_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2400 family protein"
BASE COUNT          794 a          565 c          618 g          794 t
ORIGIN      
        1 tataattgga attgcagggg tgttctcatc cctaagactt atcattttaa gaaagtccat
       61 ttcatcagga agttttgatg tggtcattcg tacatcttct tttatcaatt taatattgtt
      121 gatattttta gatgtcccta ctttagctaa agttttgttt ttaggtatat cggttttgct
      181 tttcttaata gatatttata acaagcattt tcagaaggtg gatgagtaat ggttgaaaat
      241 tgtgcggata aaacctgaat gttaatggaa caatattaat tcagctactc catcaatttt
      301 cacgataata aaattgtctc catttaaaga aaatgttgtt ttccgataac catatcggaa
      361 taatgaatag taatcttagc gttctttgtg taatgtcttt gtgtgctttg tggtttctaa
      421 cattatttaa tcataaagtt cacaaaaaat gggtgcttca tatagcgata agcttcacat
      481 tgttttttta aaatactgaa aaacatatgc ggtttttaac tatgttgaag ctagttggcc
      541 aacatgcttg aagcatgggg tgaaaccttc gggaagtaaa tccatatttt aatttattta
      601 tcggaaatcg gtgaacgaat ttaaaactta atccaaaaaa attcagtata agtacatata
      661 tttgtactca aatagtaacc taaaattttt agattatgct cgcagcaaat tttactgaat
      721 ttagggtagg cctgaaaaag ttcctggacg aagttgagat gaacaatgaa actcttataa
      781 ttaagagggg agcaggaatg ggtgccgtga tgatgtcgct tgaagagtat aattccataa
      841 tggaaacctt acacttgctg agttcaaagg ctaatgcgga aagactcttt gaatccatta
      901 accagatgaa cgaacggaaa gtggtaaaag gcaaactttt agagtaatga ctagaattac
      961 cttctccaga aattcgtggg aggagtacaa gtcgtggcag gttgatgaca ggcttatact
     1021 aaaaaagata aacgaactta ttaaggacat agaaagacat ccttacgagg ggttaggcaa
     1081 acctgaacct ctgagattcg atctggcggg tttttggtcc aggcggattg atagggagca
     1141 tcgcctggtt taccgggtta tagaccagga aatattgatt tacagctgca gataccacta
     1201 cgataagtag tgcatagcta ttttcttgta ctgagcttgc caaccttaag gttttatgtg
     1261 ctaactcatt aataaactac caagtaaggc aagttgcaca gaaacgtgaa aatgagcaat
     1321 aggtatttgc ctacggcgag cacttcgggg ttcatccgaa cgttaaccag tgtaaataca
     1381 ccaattcgta acataatgtc agccattgca ttaatgctgt atactgatga aatattcctt
     1441 ttggtttact agatcgatga aaaaaaactt taccgtacat aggccctttc gggagcaccc
     1501 ctttgcagct tcggggcagt agtggtatcc tgcccaacat atttggcttt ttaaatcagc
     1561 ctaaaaggct acttggtttt ataaaccgga gttctctcct tatgccaggg gaatttccac
     1621 acagctatac tcattcagaa aatccgagtg gcaagggctg ctaaaaggca atgtagaact
     1681 ccgaggtgag gctcctgtac tcttttgaga acaagccatc ggccgtgtga atgcagaggg
     1741 acgattctgc ggtggccatc ctttcagcct ccagctgaac cagcattcgc aatgttctgc
     1801 caccaggctt ggacagtatg tttagttttt tcgagcagaa caggccatag tgtgatgcct
     1861 ttgcaataaa aacgttgccc tcggcgtagg ggaaaattcc acaaaaccgt ccacctggca
     1921 gcatcagctt tgtagctccc tcaagcaggg caggggtagg cagggtgtcg gtatggcggg
     1981 aaagggttct cttcgaatcg ggtgatttaa gcgaatggtt gaagaagggc gggttggaga
     2041 caataaggtc gtatctgaat tcagcgcagg aggcaaaatc ctgaaacgag gtgcaaacta
     2101 cccttatcct gttggcccag ggcgatcggg cggcgttgcc tgctgcctcc tcggccgatg
     2161 gcgggtcaat ctccactgca tcaaccatgg cgtttgggct acgctgagcc accataagcg
     2221 caataactcc tgtcccggtg cccacatcca gaattctttg gacccccgag acatctgccc
     2281 atgcccccag tagcacccca tcggtgttaa cccgcatggc cgatttttcc tggaacactt
     2341 taaactgctt aaacctaaag tagctgtttg ccatttcaga acatctatgt ggtttagatt
     2401 tcgccgtgaa cccccatgcc gaaaagcgca aagtcgtact tcacggggtc gtcagcgtca
     2461 aatccacgca gggattcagt gagttcctct acagccttaa aatcgttttg tttgcgggta
     2521 agcagcccca gtgctcgggc aaccctgccg ctatgaacat cgagcgggat aagtagggct
     2581 gacgtgggca tttcgctcca gaggccaaag tctatcccct cttttggcgg cctaaccatc
     2641 caccgcagga acatgtttag tcggataatt agtgtagcca ttaatcaaaa tatattaact
     2701 cggtcggatt aatttttata atatcaacag agtttgaacc accacctgct tcgaatccga
     2761 caaaatcttt c
//
LOCUS       DAOOQX010000220         2762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_44282_length_2762_cov_2.480236, whole genome shotgun sequence.
ACCESSION   DAOOQX010000220 DAOOQX010000000
VERSION     DAOOQX010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44282_length_2762_cov_2.480236"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..2272
                     /locus_tag="PLX53_04495"
     CDS             227..2272
                     /locus_tag="PLX53_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY72371.1"
                     /translation="MNIFVFNITLLEGIAMNKLTLTRRIALLACFAIAANISFAQTSK
                     LAKADALYKAGAYFEAIDLYKADIDKINDKNELPKYLYKLGNCYRLTGNARQAELWYQ
                     KAIMREYPDPKVYFYYAEMLKMGEKYNEAIEQYQKYKVAVPLDPLADAGIKSCELSIK
                     WKETPSGYEITNMRSLNTRTSEFSPAYASSDYTTLFFTSSRNEATGGKVSSVTGENFT
                     DIFISERDNKNVWSKPRPIDETINTSYDEGTCALTSDYNTLFFTRCKVSKRDKLGCQI
                     LVSKRAEQGWLTPDELKLAADSMIVAHPSISADGTELYFVSNISGGIGGLDIWKITRS
                     SSTSDDWGEPVNLGGEVNSAGNEMFPYIHPDGTLYFSSDGHPGMGGLDIFRSWVNDEG
                     RREVENMRYPINSSADDFGIVFEKDREAGFFSSRRQGGRGADDIYMFYLPPINFNLIG
                     HVTDEKSKKPVEQATVKLVGNDGSVVTMQTLADGSFRFNLTPNADYVVIASKKGYLNN
                     KAKESTRGLTQSKDFEVSIALTSTEKPIEIPNIFYDYDRWELRPESREALDRLVEILN
                     DNPNIIIELASHTDSRGTKEYNYELSQKRAQSVVNYLIEKGIEPVRLKAKGYAQTQPK
                     VVDDALASRYPFLQVGTVLDQTFIDTLTSDEQKEIVYQINRRTEFRVLKEDYSAKPK"
     gene            2379..>2762
                     /locus_tag="PLX53_04500"
     CDS             2379..>2762
                     /locus_tag="PLX53_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72372.1"
                     /translation="MKVATYHLLAAFLLPAFAAGQIAESRVTFDISAAEAFIGTFEAM
                     QQGKSEKQVDSLIDGMLALHAYAVSAERFGAKNQISPVSLEEYKRFLKSFISDSVDVQ
                     GNTRLGMLKSRYVDAYSNLPDYKKLV"
BASE COUNT          757 a          644 c          650 g          711 t
ORIGIN      
        1 taattgaaac gccctgtggt tttggttttg gatgttttgg ttaaattttg caatttcttg
       61 taggtttgca tagctcctgc attacattaa acgtcgcaat aatccattat agaacgaagc
      121 agttaaccaa cagcttcgtt ctatttttta tcaccctatc ataacttttt cctgttccgt
      181 tgatatttga aatcgtggtg agctaattca tggtggaata aatattttga atatttttgt
      241 attcaacatt accctacttg aaggaatagc catgaataaa ttaaccttaa cccgacggat
      301 agcccttttg gcatgttttg ctatagctgc aaatatatcg tttgcccaaa ccagcaagct
      361 ggctaaagcc gatgccctgt ataaggccgg agcctatttt gaagcaatag atctgtacaa
      421 ggctgatatc gataagatta acgataagaa tgagttacca aagtatcttt acaagctggg
      481 caactgctac cggctcaccg gtaacgcccg acaggccgaa ctctggtatc aaaaagccat
      541 tatgcgtgag tatcccgacc ctaaggttta cttctactat gctgaaatgc ttaaaatggg
      601 cgaaaagtac aacgaggcca ttgagcagta ccaaaagtac aaggtggctg ttccgcttga
      661 ccccttggct gatgccggta taaaatcatg tgagctatcc attaagtgga aggaaacacc
      721 ctcggggtat gaaattacca acatgcgtag ccttaacacc cgtaccagtg agtttagccc
      781 tgcttatgct tcgtccgact ataccacgct ttttttcacc agctcacgca atgaggcaac
      841 aggcggcaag gttagctcgg taacgggaga gaactttacc gatattttta ttagcgaacg
      901 tgataataag aatgtttgga gtaagccacg ccctattgat gaaaccataa atacttccta
      961 tgatgaggga acctgcgccc taacctccga ttacaacacc cttttcttta cccgttgcaa
     1021 ggtgagcaag cgcgataagc tgggatgcca gatattggtt tccaaacgag ccgagcaggg
     1081 ctggcttaca cccgatgagc tgaaacttgc cgccgacagc atgattgttg cccatccttc
     1141 aatttcggcc gatggcaccg agctgtactt tgttagcaac atctccggag gcattggggg
     1201 gctagatatt tggaagatta cccgaagcag ctcaacctcc gacgattggg gtgaacccgt
     1261 taaccttgga ggcgaggtaa attctgcggg caacgagatg ttcccctaca ttcaccccga
     1321 tggaacgctc tacttttcgt ccgacgggca ccccggaatg ggcggtcttg acatcttcag
     1381 gtcgtgggtg aacgatgagg gtaggaggga ggtggaaaac atgcgctacc ccattaactc
     1441 ctccgccgac gatttcggaa ttgtgtttga aaaggatcgc gaggctggtt ttttctcctc
     1501 acgccgtcag ggtggcagag gcgccgacga tatctacatg ttttaccttc ccccaatcaa
     1561 cttcaacctg attgggcatg taaccgatga gaaaagcaaa aagcccgttg aacaggctac
     1621 cgttaagctg gttggaaacg atggttccgt ggttaccatg caaaccctgg ccgatggctc
     1681 tttcaggttc aacctcactc ctaacgccga ctacgtggtg attgcctcca agaagggcta
     1741 cctcaataac aaggcaaagg aatcgaccag agggcttacc cagagcaagg actttgaggt
     1801 ttccattgcc ctaacctcca ccgaaaaacc gatcgagata cctaatattt tctacgatta
     1861 cgataggtgg gaacttcggc ccgaatcgcg tgaggcgctt gaccgactcg tggaaatcct
     1921 gaacgataac cccaacatca tcatagagct ggcctcgcac accgatagcc ggggaaccaa
     1981 agagtacaac tatgagcttt cccagaaacg cgcccagtcg gtggtgaact acctgattga
     2041 gaaaggtata gagccagtta ggctgaaggc caagggatat gcccaaaccc aacctaaagt
     2101 tgttgacgat gccctggctt ccaggtaccc cttccttcag gttggaacag ttttagacca
     2161 gacgtttatc gatacgctca cctcggacga gcaaaaggaa atagtttacc agattaaccg
     2221 tcgaaccgag ttcagggtgc ttaaggagga ttactcggca aaacctaagt agctacaaca
     2281 atccctgtaa gcatagaaat tgttggtcga aaacttataa atggagttca attgatgatt
     2341 tattaaggat gaaaggtaac cgtttaaccg caataaagtt gaaagtagct acttaccacc
     2401 tgcttgcagc ttttttgctg ccggcttttg cagctgggca aattgcagag tcaagggtta
     2461 ctttcgacat ttcggctgct gaggctttta ttgggacttt tgaagccatg cagcagggaa
     2521 aatcagaaaa acaggttgac agccttattg atggaatgct tgccctacat gcctacgctg
     2581 ttagcgccga acgctttggt gcgaagaatc aaatttcgcc cgtttcgtta gaggagtata
     2641 agcgctttct gaaaagtttc atctccgatt cggttgatgt ccagggaaat accagattgg
     2701 gaatgctaaa aagccggtat gttgatgcct atagtaacct cccggattac aaaaagcttg
     2761 tg
//
LOCUS       DAOOQX010000221         2757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_44373_length_2757_cov_3.812731, whole genome shotgun sequence.
ACCESSION   DAOOQX010000221 DAOOQX010000000
VERSION     DAOOQX010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44373_length_2757_cov_3.812731"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLX53_04505"
     CDS             <1..620
                     /locus_tag="PLX53_04505"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOY72373.1"
                     /translation="THVEATGRIGFFKILSETAIAAGVRRIEAITGIKAEEFVYSQED
                     TIKEIKSLLGNPSNVSQTVARMVADNEMLRKEISSLQAEKLAEVKHQLKQSIEQRNGI
                     NVICQKVGLNDSTTIKDLAFQLRNEVPNLFLAIGATASEKAILTVMISDNLVQSLQLN
                     ATSIIREAAKEIQGGGGGQPFFATAGGKNPAGLDAALAKTLEFLE"
     gene            857..1708
                     /locus_tag="PLX53_04510"
     CDS             857..1708
                     /locus_tag="PLX53_04510"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOY72374.1"
                     /translation="MRQSGVRCIIFLLLPLVVVSCLKPGSYNIRERYVVKAKTLIVRS
                     SPSTASAPVVPLQQGDTINAIVSDTEWVTVKVGTKKGYVAASYLNRIEPTPTPNLLSW
                     VEKTASWESWVFWLIAIPAIVIWIFSGKLIVQYKNRLAQKHDFQVKGLVLMPVVFFIN
                     ALLLAILYINWKDLLLNSIEHGIRAIPNNPDIIDWVVWCQALSLLVSILVDLLGTVIK
                     SGILWGVVLTFVDLLWSVFIFAATFFLTLSLYYYAIVFLLIFFGIQYVSMVYQNSRKI
                     SSYSRPR"
     gene            complement(1710..>2757)
                     /locus_tag="PLX53_04515"
     CDS             complement(1710..>2757)
                     /locus_tag="PLX53_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY72375.1"
                     /translation="VCKKFQDFSLNQISFTVDQGDYFMLLGPSGAGKSLILETIAGLH
                     KPDSGKIFFQGVDITHIGVRERNFGIVYQDYAVFPHLTVYGNIAYPLRRKGIRGKELH
                     KQVTRFAERVSVAHLLTRHPQTLSGGEVQRTALARTLAMEPRILLLDEPLSSVDVKLR
                     SELRSLLRSINSSGQTVIHVTHDYEETLALASHLAVVNGGCIEQVGSVQEIFQNPRSE
                     FAAMFAGVHNFYPASIVEFSEHGLTTVRLNDIIAICCYTEGCNGEGFVCFSEESVILS
                     TEKPHQSALNVFRGRVVEIFPQRFGLMAVVDAGFRVLSKVTWESASRFGLTPGVEVWV
                     SFKASSVRFIPRAS"
BASE COUNT          777 a          635 c          606 g          739 t
ORIGIN      
        1 gcacacatgt tgaagcaaca ggtagaatag gatttttcaa gattttatcc gaaaccgcta
       61 ttgctgcagg agtaaggcga attgaagcta ttaccgggat aaaagcggag gagtttgttt
      121 actctcagga ggataccatt aaggagatta agtccttgtt gggaaacccc tccaatgtat
      181 cccaaaccgt tgcccgtatg gtagccgata acgaaatgct acgaaaagag atttcaagcc
      241 tgcaggctga aaagttggcc gaagttaagc accagcttaa gcaaagtatt gaacagcgaa
      301 acggaatcaa cgttatttgc caaaaggtag gcctgaacga ttcaactacc atcaaggatt
      361 tagcctttca gctaaggaat gaagttccaa accttttcct ggctatcggg gcaacggcaa
      421 gcgaaaaggc aattctaaca gttatgataa gcgataacct tgtccaatct ttgcagctaa
      481 acgcaacaag cattattcgt gaggcggcta aggaaattca agggggcggt ggtggccaac
      541 ccttttttgc aacagccgga gggaaaaacc cggcaggtct ggatgccgct ttggccaaaa
      601 cattagaatt cctggagtaa gatttttaga ctcttaattt tacttaggtg ttaacataga
      661 ctaagcatag ctttcagctt taagaaaaaa gaagaactta tcctgttacc ttctgaaggg
      721 tgtttagctt ttaatgaaat gactagcaaa cagtagaacc gcttcccctt taaccattag
      781 gtttattttt taaactaatt tttttgctgc atttgcttca gacattacct ttgtgctgga
      841 ttcaactttc tatactatga ggcaaagtgg ggttaggtgc attattttcc tcctgttacc
      901 attggtggtt gtctcatgcc tgaaacccgg gagctataac attcgagaac gctacgtggt
      961 aaaggccaaa acgttaattg ttcgcagctc cccctcaacc gcttctgccc cagttgtccc
     1021 ccttcaacag ggcgatacga taaatgctat tgtctcggat accgaatggg tgacggttaa
     1081 ggttggcact aaaaagggat atgttgcagc ttcctacctc aaccggattg aacccacacc
     1141 aactccgaat ctcctaagct gggttgagaa aacagccagc tgggaatcgt gggtgttctg
     1201 gctaatagct attcctgcaa tcgtgatttg gattttttca ggcaagctaa tcgttcagta
     1261 taaaaaccga ctggcccaaa aacatgattt tcaggtaaag gggcttgtac taatgccggt
     1321 ggtttttttt atcaacgcat tgctcctggc aattctatac atcaactgga aggatttact
     1381 acttaacagc atagaacatg gcattagggc tattccaaac aatcccgaca taatagattg
     1441 ggtagtttgg tgtcaagccc tatcattgct ggtaagcatt ttggttgatt tactcggaac
     1501 ggttattaaa agcgggattc tatggggagt ggttcttact tttgttgatt tgctctggag
     1561 cgtttttatt tttgcagcga cttttttcct cacactttcg ctctactact acgccatcgt
     1621 attcctgttg atattttttg gcattcagta tgtttcaatg gtttaccaga atagcaggaa
     1681 aatttcatca tatagcaggc ccaggtaaat tagctggccc tagggataaa ccttactgag
     1741 cttgccttga acgataccca cacctcaaca ccgggggtaa gaccgaaacg gcttgccgat
     1801 tcccaggtta ctttcgaaag caccctaaac ccagcatcaa caacagccat taggccaaaa
     1861 cgctggggga aaatttccac taccctaccc cgaaaaacat taagagcact ctgatgcggt
     1921 ttctcggttg agaggataac ggactcctcg gaaaagcaaa caaagccctc tccattgcaa
     1981 ccttcggtgt agcagcatat agcaataatg tcgttcaacc tgaccgttgt taatccatgc
     2041 tccgaaaatt ctactatact agcggggtaa aagttatgaa ctccggcaaa catggcagca
     2101 aactccgagc gaggattctg aaaaatctcc tgaacacttc caacctgctc aatgcaaccc
     2161 ccgttaacta ctgccaggtg ggatgcaagc gcaagggttt cctcgtagtc atgggtaacg
     2221 tgaatcaccg tttggccgct gctatttatg cttctaagca gggagcgtag ctcagaccga
     2281 agcttaacat caacagacga aagaggttcg tcgagaagca ggatacgtgg ctccatggcc
     2341 aatgtacgag ccagagcagt tcgctgcacc tcccctcccg aaagagtttg aggatgcctg
     2401 gttagcaaat gcgctaccga aaccctttcg gcaaaccttg ttacctgctt atgcaactcc
     2461 ttacccctta tacctttccg gcgaagcgga taggcaatgt tgccgtagac tgtaaggtgg
     2521 ggaaaaacgg cataatcctg atacacaatt ccaaaatttc tctccctaac cccaatatgg
     2581 gtaatatcga caccctgaaa aaatattttt ccggagtctg gtttgtgcag tccagcaatg
     2641 gtttcgagaa ttaacgattt tcctgccccc gatggcccca acagcatgaa gtagtcccct
     2701 tggtcaaccg tgaacgaaat ctggttcaag ctaaaatcct gaaacttttt gcaaacc
//
LOCUS       DAOOQX010000222         2755 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_44428_length_2755_cov_2.845556, whole genome shotgun sequence.
ACCESSION   DAOOQX010000222 DAOOQX010000000
VERSION     DAOOQX010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44428_length_2755_cov_2.845556"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2755)
                     /locus_tag="PLX53_04520"
     CDS             complement(<1..>2755)
                     /locus_tag="PLX53_04520"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOY72376.1"
                     /translation="DRGAIYQISITFNKKQLLSSCGDDSDQTTPTSPFSPGDEEVARG
                     YDGPGYYDDYYYYYDDDYNWRERDNPCNSAYYSESRMINQIIISSDIGIIAKLGNDGG
                     MLVAVTDLPSAKPLSNANVRISDYQNQTIADGNTNNDGFFEFKTTRRPFLVTASDGKQ
                     KGYLRVDNGTSNPLSNFDVSGNEVQHGLKGMIYGERGVWRPGDTLHLCFILEDKGKTL
                     PPNHPVVLELHDPKGLLVRRAVQPNNDAGIFYFPTVTDPQAPTGPWSATVRVGGAIFQ
                     KTLKVESIKPNRLKINLELQGNNIPPSGMVNATLNAKWLHGAAAANLKAKYELTVSKA
                     NASFPEFSSYSFDDPGIDFPTQTQTLWDGQLDDNGTARVYGSLNIPDSRPAAINVLFK
                     GRVFEPGGDFSIDLFSKTFYPFSELVGVKPAQPKMGTSWLETDTDQQFSVVTINNDGK
                     PISCPNLELELYKLTWRWWWEQPETGEAGYVNRNYDYLISSHKFGTVNGKATVKLRVD
                     YPEWGRYYVKVTNKTTGNSCGTIVYFDWPYSSGKSQANRPGGASILAISVDKPDCKVK
                     ESFKLTIPGAENSRALVSIENGYKVVKAFWVDASSQANVVDIQTTPEMTPNVFVHVTL
                     IQPHTNKTNDLPIRLYGITRINVSDPETTLQPVITMPDELKSDKPVEILIREKSGKKM
                     NFTLAMVDEGLLDINRFKTPDPHSVFYAREAIGVKTWDLFDEVIGAYGSTMERLISVG
                     GDEELNPTQGEKIQRFKPVVRFFGPFVLEKGKTEKISFNMGNYFGSVRIMVVAANDGA
                     YGFAEKSCPVKNDLMVMATLPRVLGPQETVTMPISIFTTSPAIKQVKIRVKTSSNLKV
                     LNGNEQNLEFTKPGDLLAFFTLQVSEGTGPARVEVLAEGNGVKSTQVVDIEVRNPISI
                     QVVS"
BASE COUNT          627 a          648 c          654 g          826 t
ORIGIN      
        1 tgcttacaac ttgaattgaa atgggattac gtacttcaat gtcaactacc tgggttgatt
       61 ttaccccgtt gccttcggca agcacttcaa cccttgccgg accggtgcct tcggaaactt
      121 gtaatgtgaa gaatgctaac aggtcgccag gttttgtaaa ttctaggttt tgctcattac
      181 cattcagaac tttcaggttt gaagaggtct ttaccctaat cttcacctgc ttaatggcgg
      241 gcgatgtggt aaagatgctg attggcatgg ttactgtttc ctgaggtccc agtacccggg
      301 gaagggtggc cattaccatc aggtcatttt ttaccggaca acttttttca gcaaaaccat
      361 aggctccatc gttggctgca acaaccatta tccttacgct gccaaaataa tttcccatgt
      421 tgaagcttat tttttcagtt ttaccctttt cgagcacaaa ggggccaaag aatcggacaa
      481 ccggtttgaa gcgctggatt ttttctcctt gggtaggatt tagctcctcg tcgccaccta
      541 cagaaatcaa cctttccatt gtgcttccgt aagcacctat gacttcgtcg aacaaatccc
      601 atgttttcac ccctatggcc tcccgggcgt aaaataccga gtggggatcg ggggttttga
      661 agcggttgat gtcgagcaac ccctcgtcaa ccatcgcaag cgtaaagttc atttttttcc
      721 cgctcttctc cctgatgaga atttcgacgg gtttatccga ttttaactca tcgggcatag
      781 tgattactgg ttgcagcgtt gtttcggggt cagaaacgtt aatccgggtg attccataga
      841 gacggatggg caagtcgttg gtcttattgg tatgtggctg tatcagggta acatggacga
      901 acacatttgg ggtcatctcc ggtgtggttt ggatgtcaac cacgttggcc tggcttgaag
      961 catcaaccca gaatgcttta accaccttgt acccattctc aatgctaaca agagctctcg
     1021 aattttcggc gccaggaatg gtaagtttga agctttcttt aactttacaa tcgggcttat
     1081 caaccgagat agccagaata ctggcaccac ccggacgatt agcctgagat tttcctgaac
     1141 tgtatggcca gtcgaagtat actattgttc cgcagctgtt ccccgttgtt ttattggtaa
     1201 ccttaacgta gtagcgcccc cattcgggat aatcaaccct aagcttaacg gtagccttgc
     1261 cgttgactgt tccaaattta tggctcgata taaggtagtc gtagttccgg ttcacgtagc
     1321 cggcctcgcc tgtctcgggc tgttcccacc accatcgcca cgttagcttg tagagttcca
     1381 gctccaggtt cggacagctt atcggcttgc catcgttgtt gatggtaact accgagaatt
     1441 gctggtcggt gtcggtttcg agccagctgg ttcccatttt aggttgtgct ggcttaactc
     1501 ccacaagctc gctaaacggg taaaatgttt tagaaaacag atcgatgctg aaatcaccac
     1561 ccggttcaaa aactcttccc ttaaaaagca cattaatagc ggctgggcgg ctatcgggta
     1621 tgtttagtga tccatagact ctggcggtgc cattatcgtc gagttgtcca tcccacaagg
     1681 tttgtgtttg cgttggaaaa tcaattcccg gatcgtcaaa tgagtagctg ctgaattcgg
     1741 gaaacgaagc atttgccttg cttacagtta actcgtattt agcttttaga tttgccgcag
     1801 ctgcgccatg cagccatttg gcgttaaggg tagcgttgac catgcccgac gggggtatgt
     1861 tgttgccctg aagttcaagg tttattttaa gacggtttgg ttttatactt tcaaccttta
     1921 aggttttttg aaaaatggct ccgcccaccc taactgttgc gctccatggt ccggtaggag
     1981 cttgaggatc ggttactgtt ggaaagtaga aaatccctgc atcgttgttg ggctgaacag
     2041 ctctcctgac cagcaaaccc ttcggatcgt gcagctcaag tactaccggg tggttcggcg
     2101 gaagtgtttt tcccttgtcc tcaagaataa agcagagatg aagggtgtcg ccggggcgcc
     2161 atacacccct ttcaccgtat atcattccct ttaagccatg ttgaacctca ttcccgctaa
     2221 cgtcgaagtt gcttagcggg ttcgatgtcc cgttatctac acgcaagtat cctttttgct
     2281 tgccatcact tgcggttacc aaaaatggcc ttcgtgttgt tttaaactca aaaaatccgt
     2341 cgttgttggt gttaccatcg gctatggttt ggttctggta gtcggaaatt cttacgttgg
     2401 cgttagacaa cggttttgct gaaggcaagt cggtaacagc aaccagcatt ccaccgtcat
     2461 tacctagctt tgcaataata ccgatatctg atgatattat tatctggtta atcattctcg
     2521 attctgagta gtaggccgag ttgcaggggt tgtctctctc tctccagtta taatcatcgt
     2581 cgtagtaata gtagtagtcg tcgtagtaac cgggtccatc gtaaccccgt gcaacttctt
     2641 catcgccggg gctaaaagga gatgtaggag tggtttggtc ggagtcatcc ccgcaggaag
     2701 aaagaagttg ctttttattg aatgtaatgc taatttggta aattgcgccc ctgtc
//
LOCUS       DAOOQX010000223         2738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_44825_length_2738_cov_2.561685, whole genome shotgun sequence.
ACCESSION   DAOOQX010000223 DAOOQX010000000
VERSION     DAOOQX010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44825_length_2738_cov_2.561685"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PLX53_04525"
     CDS             <1..367
                     /locus_tag="PLX53_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOY72377.1"
                     /translation="RGLMAILLLVISNTFMTMAWYGHLKFKELKWFQNFGIVGIVLIS
                     WGIALFEYFFQVPANRLGYKENGGPFSLLELKVIQEVITLLVFIIFTVLFFKTESIRI
                     NHLIGFCFMVLAVYFVFKK"
     gene            780..1175
                     /locus_tag="PLX53_04530"
     CDS             780..1175
                     /locus_tag="PLX53_04530"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOY72378.1"
                     /translation="MDSLYIEAEIDSPEVYFDPETKIFSISGISHPENAKEFYQVIME
                     WVDEFYEYMKNKKPCKIIVDMNFKYINSSSYKYLRELLIKMANFKNSHFDIEVVWNYQ
                     EEDEDLLTEGIVLFELPEINLPYRCVSYR"
     gene            1471..2559
                     /gene="hydE"
                     /locus_tag="PLX53_04535"
     CDS             1471..2559
                     /gene="hydE"
                     /locus_tag="PLX53_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOY72379.1"
                     /translation="MAFIEELLAKESYTKSELVELLQVTGDGRAKLFSKAAGIKEKYL
                     GNKVYLRGLVEMSNVCRKNCLYCGIRSGNQKVNRYNLTDDEIVDAAIFAWKNKYGSIA
                     LQAGEISTREFATRVEGLLGKINAATYDELGITLSLGEQNEDTYRRWFQKGGIRYLLR
                     IESSNQELYKKLHPNDSLHDYKTRIDCLKSLRRVGYQVGSGVMIGLPFQTLEHLADDL
                     LWMQEMDLDMVGMGPYVEHPDTPLFEYRDQLLPVAERFDLSLKMTALIRIMMKNINIV
                     APTALQAIDPLGREKAIKVGANVVMPNITPGAFRNDYKLYENKPCVDDAADDCKNCLD
                     ARIQLAGHEVGYGEHGDSPHFYQRKNAQ"
BASE COUNT          847 a          577 c          576 g          738 t
ORIGIN      
        1 cagagggttg atggcaattt tactgcttgt tatctcgaac acctttatga ccatggcatg
       61 gtatgggcat ttgaaattca aggagctcaa atggtttcaa aattttggta tagttggcat
      121 agttctgatc agctggggta tagccctctt cgagtatttt tttcaggttc ctgccaaccg
      181 tttaggttac aaggaaaatg gcggcccatt ttcactcctg gagctaaagg ttattcagga
      241 agtgattacc ctgctggtat ttataatttt caccgtgcta ttcttcaaaa ccgaaagcat
      301 aagaataaac cacctaatcg gcttttgctt tatggtgcta gcagtttact tcgtattcaa
      361 aaagtgatgg gttgtgctgg tactaaaacg aagaaggcct ttcggccttc ttctccccaa
      421 acacacgatt tactgaaaaa accaactggt ttccatcaaa ctacctagaa aaaatataca
      481 aactaacctc ccagtaaact atggaaacca aactgaatag gacaaatata ataccaaatt
      541 tttattgaat gaaattataa cgaaaattat tactacgaaa aaattgaaaa atttttaacg
      601 actgttgttt attatgaacc acaccctgta taattaaaat tttcaacttt ccctttgcca
      661 tttatctact tataccggta cttcgtttta cgttttagga cattaatcaa agaaaaggcc
      721 ttacagtttg cccaagctgt aattatgtaa tatctttacg ctgataagca aaaataaata
      781 tggattccct atatattgaa gcagaaatag actcccctga agtttacttt gaccccgaaa
      841 ccaagatttt ttccatttcg ggcatctcac accccgagaa tgccaaagaa ttttaccagg
      901 ttatcatgga gtgggttgac gagttttacg agtacatgaa aaacaagaag ccttgcaaga
      961 ttatagtgga tatgaacttt aaatatatca actcttcatc gtacaagtac ctaagagagc
     1021 tgctcatcaa aatggctaac tttaaaaaca gccactttga tattgaggtt gtatggaact
     1081 accaggaaga ggatgaagac ctgctaaccg agggcattgt tcttttcgaa ttacccgaaa
     1141 taaacctgcc ctaccgatgt gtatcatacc gttagcataa tcggccaact cacaacccgg
     1201 ctacactgct ctgtgctgca atacatttga ccaagccaac attttaatga atgctgtcaa
     1261 tcatccattt attaaggatg aaatataaat ggtcaaaaat ccaattttta tacctttgtg
     1321 ctgttgcagc caaccatttt catgggattt tcagcccaat atttgattta ccccggcaaa
     1381 atcgaagttg aaagaaggct gtcagccaaa taacagggct atgggatagc atgggctcta
     1441 ccacttgaaa aattaaagaa tacaaagctt atggcattta tagaggaact tttagcaaaa
     1501 gaatcataca caaaaagtga gctggtagaa ctcttgcagg ttaccggtga tggaagagct
     1561 aagctcttta gcaaagcggc cggaataaaa gagaagtatc tgggtaataa ggtttacctc
     1621 cgtggactgg ttgaaatgtc gaatgtatgt cgtaagaatt gcttatactg tggtatcaga
     1681 tcgggtaacc aaaaggttaa tcgctacaac cttacggacg atgaaatagt ggatgcggca
     1741 atttttgcct ggaagaataa atacggaagc attgccctgc aggcaggtga aattagcacc
     1801 cgcgagtttg caacgcgggt tgaaggcttg ctcggtaaaa ttaatgctgc aacatacgac
     1861 gagcttggca tcaccctttc cttaggtgaa cagaatgagg acacataccg acggtggttt
     1921 caaaagggag gaatacgcta cctgctgcga attgagagtt caaatcagga gctctacaaa
     1981 aagctgcacc ctaacgatag cctgcacgat tacaaaacaa gaatagattg ccttaaaagc
     2041 ctccgtcggg ttggctacca ggtaggctcg ggcgtaatga tcgggttgcc attccaaacc
     2101 cttgaacatc tggccgacga ccttctctgg atgcaggaga tggacctcga catggtgggc
     2161 atgggcccct acgtggagca tcctgatact ccgctcttcg agtaccggga ccagctgcta
     2221 ccagttgccg agcgtttcga cctctcgctc aaaatgacgg cgctaatcag gataatgatg
     2281 aaaaatatta acattgttgc gccaaccgcc ctacaagcca tagatcctct tggaagggaa
     2341 aaagccatta aggtgggggc caacgtggtg atgcccaaca ttactccagg agcatttcgt
     2401 aacgactaca agctatacga gaataaaccc tgcgttgacg atgctgctga tgattgcaaa
     2461 aattgtctgg atgcccgcat acagcttgcc gggcatgaag ttggatatgg cgaacatggc
     2521 gattcaccgc acttctacca gagaaaaaat gcccaataaa tttagctgta taaattctga
     2581 cgtgccgtgt aaataaccac aaacaaataa atcgtaaagg atacccctaa agcttcaccg
     2641 cgctgccggt gtttttccct cgctgttaca aatgaatggg attctgatgg ctttcccctt
     2701 ttgctacatg cctaattgaa gacatggcgg ggtgagag
//
LOCUS       DAOOQX010000224         2737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_44845_length_2737_cov_2.695004, whole genome shotgun sequence.
ACCESSION   DAOOQX010000224 DAOOQX010000000
VERSION     DAOOQX010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44845_length_2737_cov_2.695004"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1715
                     /locus_tag="PLX53_04540"
     CDS             <1..1715
                     /locus_tag="PLX53_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOY72380.1"
                     /translation="GKRYIDRERSNSDSSIKDIFDAVNRSINQYGHRPELAGDSTWAK
                     ENIPFAGEIYLGHLRYGTFGNNSIDYVHPVMRDNNWKSRTLVLAGNFNMTNVDELFNK
                     LVSLGQHPRDYSDTVTVLEKIGHFLDEENQQVFRQYKNEGYSNMEISKLIAENINIGK
                     ILQAATKDFDGGYAIAGLIGHGDAFVIRDPWGIRPAFYYLNDEFAVVASERSAIQTVM
                     NVKTDDVKEVNPGYGLIIKRNGTITHEEIRVPQQRRSCSFERIYFSRGSDRDIYQERK
                     KLGELLTPAILKAINYDHSNTVFSYIPNTAETAFYGMVKGVEDYLKVEKKRMILEKSS
                     SLTESDLDEVIAIRLRVEKIAVKDVKLRTFISQDKGREDLVGHVYDITYGAIRPGIDS
                     LVVIDDSIVRGTTLKESILRILDRLNPVSVVVVSSSPQIRYPDCYGIDMAKLSDFVAF
                     KAAVGLLHDTGQEAIINKVYRKCKEQQYLPKEEIVNYVKEIYSPFTDDQLAEKIAQLL
                     TPKDMRAKVKIVYQSIENLHLACPNDLGDWYFTGNYPTPGGNRVVNNSFINFIEGRNN
                     RAYQ"
     gene            complement(1800..>2737)
                     /locus_tag="PLX53_04545"
     CDS             complement(1800..>2737)
                     /locus_tag="PLX53_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOY72381.1"
                     /translation="DRNLLMAELTPEKFVRNNNNAENEPFLFTAHDSPHLMREVGRLR
                     EFTFRSAGGGTGEEVDIDEYDICDNCYKQLIVWDPKEREILGGYRFHSVDPSKGDEIN
                     LSTKHLFNFSEKFVREYIPKMIELGRSFVQPKYQSLSRGGKGLYALDNLWDGLGALIV
                     LNPHIRYFFGKVTMYRSYNVEARDTLLYFFEKYFKDNEQLVTTIHPIEVNMDRDKMER
                     LFTGKNYTEDYKILSHKIRELGEKIPPLINAYMNLSPSMKVFGTVINPYFGNVEETAI
                     LITIPDIYQHKMERHTGTFVRKLAGKYLRPYRKES"
BASE COUNT          706 a          601 c          693 g          737 t
ORIGIN      
        1 cggggaagcg gtacatcgac agggagcgtt ctaattcgga ttcttccatt aaggatattt
       61 ttgatgccgt taataggagc attaaccagt acgggcatag acctgaacta gccggtgatt
      121 cgacctgggc taaggaaaat attccctttg ccggtgaaat atacttgggc cacttaaggt
      181 atggtacttt tggaaacaac tccatagatt acgttcatcc cgtaatgcgc gacaacaact
      241 ggaaatcacg tactctggtt ctggccggga actttaacat gaccaacgtg gatgagctct
      301 ttaacaagct ggtaagcttg gggcagcacc cacgtgatta ctccgacacc gtaactgttc
      361 tggaaaagat tggccacttt ctggatgagg aaaaccagca ggtttttcga cagtataaga
      421 atgaggggta ctccaacatg gagattagca agctaattgc cgagaacatt aacattggca
      481 agatacttca ggcagcaaca aaggattttg acggggggta tgccattgcc ggtcttattg
      541 gtcatggcga tgcctttgta attcgcgacc catggggcat tcgtccagcg ttctactatt
      601 taaacgacga atttgccgtt gtggcttctg aacgttcggc catacaaacg gttatgaatg
      661 ttaaaaccga tgatgttaag gaggttaacc caggctatgg cttgattatc aagcgtaacg
      721 gaactatcac ccatgaggag ataagggtac cccagcaacg aaggtcatgc tcattcgaga
      781 ggatttactt ttcacggggc tcagacaggg atatatacca ggagcgtaaa aagttgggcg
      841 aactgctaac cccggcaatc ctgaaggcca ttaattatga ccattccaat actgtgtttt
      901 cttatattcc caacaccgct gaaacggcat tctacggcat ggttaagggg gtggaggatt
      961 acctgaaggt ggagaagaag cggatgattt tggaaaagag cagctcattg accgagagcg
     1021 atttggatga ggtaatcgcc attcggttac gagtcgaaaa aattgccgtt aaggatgtta
     1081 agcttcgtac gtttatttcc caggataagg ggagggagga tcttgttggg catgtgtacg
     1141 atattaccta tggcgccatc aggccgggga tagacagcct ggtggtgatt gacgattcaa
     1201 tagtaagggg aaccacccta aaggagagca ttctaagaat actcgacagg ctaaatcccg
     1261 taagcgttgt ggtggtgtcc tcctcacctc aaattcgcta tcccgattgc tacggcatcg
     1321 atatggccaa gcttagcgat tttgtagctt tcaaggccgc tgttggattg ctgcacgata
     1381 caggtcagga ggccatcatc aacaaggttt accggaagtg taaggaacag caatatctgc
     1441 ctaaagagga aattgtgaat tacgtcaagg agatttacag cccgtttacg gacgatcaac
     1501 ttgccgaaaa aatcgcccag ctattaaccc caaaggatat gagggctaag gttaaaattg
     1561 tgtaccagtc aatcgaaaac cttcacctgg cctgccccaa cgatttgggc gattggtact
     1621 tcaccggcaa ctaccctact ccgggtggaa atagggtggt gaacaactcg tttatcaact
     1681 ttattgaggg ccgtaacaac agggcatacc agtaacagtg aactttctct gtccgtgctg
     1741 tcatggtaaa tcttttgctg gttgaacccg gctgagcagg gttttctgct cccctgcggt
     1801 cagctttcct ttcggtaggg tcttaggtat ttaccggcca gcttcctcac gaatgtgccg
     1861 gtgtgccttt ccattttatg ctggtaaata tccggaatag ttataagaat tgctgtttct
     1921 tcaacatttc cgaagtaggg gtttataacc gtgccaaaaa ctttcatgga tgggctaagg
     1981 ttcatgtagg cgttaatgag gggggggatt ttctcgccaa gctctcttat cttgtgcgat
     2041 agaattttgt aatcctccgt gtagtttttt ccggtgaata gcctttccat tttatcccga
     2101 tccatgttca cctcaattgg atgtatggtt gtaacgagct gttcgttgtc cttaaagtat
     2161 ttttcaaaga agtagaggag tgtgtctcta gcctctacgt tgtaggagcg gtacatggta
     2221 accttgccga agaagtacct gatgtggggg ttaagcacga taagggcgcc cagcccatcc
     2281 cacaggttgt caagggcgta aagtcctttg ccacccctgc tgagcgactg gtacttaggc
     2341 tgaacaaaag agcgtcccag ctcaatcatt ttagggatat actctcttac aaatttttca
     2401 gagaaattaa acaggtgctt ggtggagagg ttaatttcat cgccctttga ggggtcaacg
     2461 ctgtggaacc tgtatcctcc aagtatttcc ctttcctttg gatcccaaac aatgagctgc
     2521 ttgtagcagt tatcgcagat gtcgtactca tctatatcaa cctcctcccc ggtgccccca
     2581 ccggcagagc gaaaagtaaa ttcccttagc ctgcccacct cgcgcatcag gtggggcgaa
     2641 tcatgggcgg taaagaggaa gggctcattt tccgcattgt tgttgttcct aacgaacttc
     2701 tcgggggtga gttctgccat tagtaggttc cggtcaa
//
LOCUS       DAOOQX010000225         2731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_44964_length_2731_cov_3.328102, whole genome shotgun sequence.
ACCESSION   DAOOQX010000225 DAOOQX010000000
VERSION     DAOOQX010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44964_length_2731_cov_3.328102"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PLX53_04550"
     CDS             complement(<1..583)
                     /locus_tag="PLX53_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOY72382.1"
                     /translation="MKKFIVLPLFCLFLSAYSQQADEINRRLEALQEENVNLNHRLDE
                     MQKQMDDVLWFNRVGDVAFVDKVYIYGPPPANVKNPTAMGATNPVKFWAYVFIPKYID
                     PSKKYPLLVFPHGGVHADFTTYHTHIIREMMAQGYIVVAAEYRGSTGYGRSFWENIDY
                     GGLENENVYASRNYIIKNYDFVDEKRVGIIGWSH"
     gene            complement(690..1946)
                     /locus_tag="PLX53_04555"
     CDS             complement(690..1946)
                     /locus_tag="PLX53_04555"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY72383.1"
                     /translation="MTFHLNPASYLSKIKFAFRALKSPNFRIFFYGQAVSLMGTFIQG
                     LALGWLVYRLTNSPFLLGAVSFAGQIPSLVLMPFAGVYADRLNRRKVLVITQGISMIV
                     ALTLATLYFTHTIQVWQIFVTSILNGIAIAFDAPFRHSFMLEMVGDKDLLQNAIALNS
                     TLVNSARFIGPAIGGVLVAWIGEGFCFLVNGISFMAVIASLLHIRVAQVERKPHNSSI
                     FKELKEGLSYSYNFLPIRYVLSLLVVVGFLGFPFQTFMPVFARDILHGNSQMLGLLTG
                     AVGAGALTGAILLASLKSIKVIPNIILYSSLTFGTSLAAFSLSSYATLSVGLLFFTGF
                     GMIVQLAATNTLLQYIVDDDKRGRILSLYSLSFNGFPPLGSLLLGAISPSVGIQPTMA
                     VAGGLCAISALLFSRRVPVVKSVVPD"
     gene            2190..2654
                     /locus_tag="PLX53_04560"
     CDS             2190..2654
                     /locus_tag="PLX53_04560"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOY72384.1"
                     /translation="MKKVKTIALVAHDHRKRDLVEWVEWNWQELLKHKLICTGTTGRL
                     IEESLQKKKSDVADHIQLTRLKSGPLGGDQQLGALIAEGKIDILIFFWDPMEAQPHDV
                     DVKALLRIATVYNIPTATNRSTADFLISSPLIDEDYNPILFDYSHYMNRKVF"
BASE COUNT          841 a          629 c          598 g          663 t
ORIGIN      
        1 catggctcca gccaatgatg ccaacccttt tctcatcaac aaaatcgtag tttttaatta
       61 tatagttacg gctggcatac acgttctcgt tctccaggcc gccatagtcg atattctccc
      121 aaaaactgcg tccatacccg gtgcttcccc tgtactctgc tgccactaca atgtaaccct
      181 gagccatcat ctcgcggatt atgtgggtat ggtaggtagt aaagtcagca tgaacacctc
      241 catgagggaa taccagcaag ggatactttt tagaagggtc gatatattta ggaataaaaa
      301 cgtaggccca gaacttaacc ggattagttg cccccatggc agtaggattt ttaacgtttg
      361 caggaggagg cccataaatg taaaccttgt caacaaaggc tacatcacct accctattaa
      421 accaaagaac atcatccatt tgcttctgca tctcatcgag cctatggttt aggttgacat
      481 tctcctcctg aagggcttca agcctacggt tgatttcatc ggcctgctgc gagtatgccg
      541 ataaaaacaa gcaaaacagc ggtagaacaa taaatttttt catctggttg taggttttgt
      601 ttgatataaa gttaaaacaa aagtttgatg aatgctacca ccaaaagctt aaccgccagt
      661 cttaaccggc tataaggaaa tgcaattttc taatcgggta ccaccgattt taccacaggt
      721 acccttctac tgaaaagcaa ggcggaaata gcacaaagtc cccctgcaac agccatggtt
      781 ggctgaattc caaccgaagg gctaatggcg cctagcaaca ggctccccaa aggtggaaat
      841 ccgttgaacg aaagactgta gagcgacagg atacgacccc gcttatcgtc gtcaacaata
      901 tactgcagga gcgtgttggt tgcagcaagc tgcacaatca tcccaaaacc agtaaagaag
      961 agtaggccaa ccgaaagcgt agcataggat gacaatgaaa aagctgccag cgatgttcca
     1021 aatgttagcg agctgtagag gataatattt ggaatgacct ttatgctctt aagcgacgcc
     1081 agcaggatgg ccccggtaag cgcaccggca cctaccgccc cggtaagcag tccaagcatt
     1141 tgcgagttac cgtgcaggat atcacgtgca aataccggca taaaagtttg gaagggaaat
     1201 cctagaaacc ctaccacaac caggagagag agcacatacc tgataggcag gaaattatag
     1261 gagtaggaca atccctcctt caactccttg aatatggatg aattatgtgg ttttctttca
     1321 acttgggcca cccggatatg aagtagggag gcaattacgg ccataaagct gattccattg
     1381 accagaaagc aaaaaccctc tccaatccat gcaaccagaa cacccccaat ggcaggacct
     1441 atgaaacgtg ccgagttaac caacgttgag tttaaagcta tggcgttttg aagcaaatcc
     1501 ttatcgccta ccatttcaag cataaacgaa tgcctgaacg gagcatcgaa agctattgcg
     1561 attccgttca aaatggaggt tacaaaaatt tgccaaacct gtatggtatg tgtaaagtag
     1621 agtgtagcca gggttaatgc aacaatcatg gaaataccct gagtaataac aagtactttg
     1681 cgcctgttca acctatcagc gtaaacgccg gcaaagggca tgagaaccag cgacggtatt
     1741 tgaccggcaa aactaacagc acccagcaaa aaaggagaat ttgtcagcct ataaaccaac
     1801 catcccaacg ctaagccctg gatgaaggtt cccatgagag aaactgcctg gccgtaaaag
     1861 aaaatcctaa agttgggact ttttaacgct cgaaaagcaa acttgatttt gcttaggtag
     1921 gatgcaggat taagatgaaa agtcattatt atttagatgg gtaggtattt ggttatgcaa
     1981 aaatacaaaa tatatgctga cgtcgtgtat tgcccatcac gcttgattga cctcccaagg
     2041 gtaggaaact taatgggtaa ggcatgttag tcattaaagt taaaaaatgt tactccccta
     2101 atcttttgtt aattctgatt tagctgtagc gcaaccatcc gtaatgtgat aatagctaca
     2161 tttgcagcaa gtgaaaaaaa cgtatttctg tgaagaaagt aaaaacaatt gccctggttg
     2221 cgcatgatca ccgtaagcgc gacctggttg aatgggtcga atggaactgg caggaactac
     2281 ttaagcacaa gctcatctgc acgggaacaa ccggtcgtct tattgaggaa tcgttacaga
     2341 aaaagaagag tgacgtagca gaccacatcc agctaacccg ccttaaatca ggcccgctgg
     2401 gtggggatca gcagctgggt gctctaatag ccgagggcaa aattgacatt ctgatattct
     2461 tctgggaccc catggaagcc cagccccacg atgtggatgt taaagccctg ctccggattg
     2521 caaccgttta caatattccc acagcaacca accgctcaac agcagatttt cttatcagct
     2581 cccccttgat tgatgaggat tacaacccta ttctgtttga ctacagccac tacatgaatc
     2641 gaaaggtatt ttaacaagtc ctttctactt caattttgag gttaagcagc gcctgaatct
     2701 ggctcgaaat ggatttcttg acagtttcag g
//
LOCUS       DAOOQX010000226         2729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_45019_length_2729_cov_2.886313, whole genome shotgun sequence.
ACCESSION   DAOOQX010000226 DAOOQX010000000
VERSION     DAOOQX010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45019_length_2729_cov_2.886313"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLX53_04565"
     CDS             complement(<1..280)
                     /locus_tag="PLX53_04565"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOY72385.1"
                     /translation="MEQFDLSLVNWSRAQFALTAMYHWLFVPLTLGLSFIIAFMETLY
                     YKTGDEEWKRITKFWMTLFGINFAIGVATGLILEFEFGTNWSNYSWFVG"
     gene            complement(287..502)
                     /locus_tag="PLX53_04570"
     CDS             complement(287..502)
                     /locus_tag="PLX53_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HOY72386.1"
                     /translation="MELPGKIFRFYYEGFRSMTVGKTLWVIILIKLFVMFAILKIFFF
                     PDILKKNFKTDTERSKYVIEQLTSNQK"
     gene            complement(595..1578)
                     /locus_tag="PLX53_04575"
     CDS             complement(595..1578)
                     /locus_tag="PLX53_04575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOY72387.1"
                     /translation="MEFRTEVAVDDFPFRLGYATPTLFCGSCFTENIGGIMRTLKMPV
                     LVNPFGVVYNPLSVAQVLQRSVAGKIYNADELQFRDGLWFSFDFHSRFASPNRDACIA
                     LLNEAVSSAKDFISTTQCIVVTFGTARVYKLKGSGEVVSNCHKVPAVEFENKLLAVDE
                     VVEIWSNLISMLLGLNPSLKLVLTVSPIRHWKDGAPGNQLSKSTLIVAVHRLVEMYSD
                     NVFYFPSYELMMDDLRDYRFYDDDMLHPSPKAIEYIWSKFSRVLIDDDSMKLAAQIQK
                     IIQAAGHRPFNTGTPEHKRFVDKILKSIQDIKLIHPSINFEREIAILKGEQ"
     gene            complement(1568..2434)
                     /locus_tag="PLX53_04580"
     CDS             complement(1568..2434)
                     /locus_tag="PLX53_04580"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOY72388.1"
                     /translation="MKNSTALINVESVHQEWLKTNGYRLFTWYIQPSSPKAAVLLVHG
                     LGEHSMRYQTWAKQFADAGFAFYCWDHVGHGQSSGQRGCVPNYEHLLQEVGFIINKFN
                     RQIGSIPLVLYGHSMGGNIALNYAIRRPSKINLLVVTSPWLKLVSPLNPALKSLVKLL
                     DLCLPFLPMNSGIRPEQVSHEMKVVQEYATDPLIHRRITPRLYLAMERAAEYAMNHSE
                     QITIPTLLMHGSSDTLLSSMATSEIAAKMHHSTYVEWDELYHELHNETKSNEVFKTIV
                     NWLNVKIAQYGV"
BASE COUNT          780 a          587 c          607 g          755 t
ORIGIN      
        1 cgcccacaaa ccacgagtag ttgctccagt ttgtaccaaa ctcaaattcc agaataaggc
       61 ctgttgccac cccaatggca aagttgatgc cgaagagggt catccagaac ttggtgatcc
      121 gtttccactc ctcgtcgccg gttttgtagt atagcgtttc catgaacgct atgataaacg
      181 ataagcctaa ggtaagcggt acaaatagcc agtggtacat tgccgttagc gcaaactggg
      241 ctctggacca gttgacaagt gataaatcga attgctccat agtcagctat ttttggttac
      301 tggttagttg ctctattacg tacttgctac gttcagtatc tgttttaaag ttcttcttta
      361 agatatcggg aaagaaaaag atcttaagaa tggcaaacat tacgaagagt ttaatcagaa
      421 taatgaccca caatgttttc cccacggtca tactcctgaa tccctcgtag taaaatcgga
      481 aaattttacc cggtagctcc atggtcaaaa taagttatga tttttcaaaa ttaacaaaaa
      541 ttaacacgaa tggttggctg tcccgattga atttaacaaa gttatctggt agaactattg
      601 ctcgccttta agaatggcta tttccctttc aaaattgatg gaaggatgaa tgagcttgat
      661 gtcctggatt gattttaaaa ttttgtcaac aaacctttta tgttcgggtg taccggtgtt
      721 aaacggccga tgtcccgctg cctggattat tttttgaatt tgggctgcca acttcatgga
      781 atcgtcatca attagaaccc ttgaaaactt actccaaatg tactcaatag cttttggtga
      841 aggatgaagc atatcgtcgt cgtaaaacct gtagtcgcgc aaatcgtcca tcatcagctc
      901 atagctaggg aagtaaaaaa cgttatcgct gtacatttca accaacctgt ggactgcaac
      961 aattagggtt gatttgctca gctgattacc gggtgcccca tccttccagt gcctgattgg
     1021 gctaacagtt agcactaact ttaggctggg gtttaacccc aaaagcatgg aaatcaggtt
     1081 gctccatatt tccacaacct catcaacagc caagagctta ttctcaaact caacagctgg
     1141 aactttatgg cagttggata ccacctcgcc actacccttt aacttgtaaa ccctggcggt
     1201 gccgaatgta acaacaatgc actgtgttgt ggaaataaaa tctttggctg aactgaccgc
     1261 ttcgtttagc agggcaatac aggcatcgcg gttaggagag gcaaatcggg aatggaaatc
     1321 aaaactaaac cacaagccat ctcggaattg caactcgtca gcattgtaga ttttacctgc
     1381 cactgagcgt tgcaaaacct gcgcaacgga taagggattg taaaccacac caaaggggtt
     1441 aaccagaacg ggcattttca acgttcgcat gatgccgcca atgttttccg taaagcatga
     1501 accgcaaaaa agggttggtg tggcatagcc cagtctaaaa ggaaaatcat caaccgctac
     1561 ctcagttcta aactccatat tgggcaattt ttacattcaa ccaatttaca atcgttttaa
     1621 aaacttcatt gcttttagtt tcattgtgaa gctcatggta tagctcgtcc cattccacat
     1681 aggtggagtg gtgcattttt gcggcaatct cgctagtggc cattgacgag agcagagtgt
     1741 cggaactacc gtgcatcaga agtgtgggga tggtaatttg ttcggaatgg ttcatggcat
     1801 actcagcggc cctctccatg gcaaggtaga gccgaggggt aattcgccga tgaataagcg
     1861 gatccgtggc atactcctgt accaccttca tttcatgcga tacctgttcg ggtcgtattc
     1921 cagagttcat gggtagaaaa ggtaagcata aatctaagag cttaaccaga ctttttaacg
     1981 cagggttgag tggacttacc agcttcaacc agggtgaagt aacaaccagc aggttgattt
     2041 tggatggtct tcttattgcg tagttcagcg caatgttgcc ccccatgcta tggccgtaaa
     2101 gcactaaggg aattgaacca atttgcctgt tgaatttatt tatgatgaag cctacttctt
     2161 gcaggaggtg ctcgtagttt ggaacacatc cgcgttgacc gcttgattgc ccatgaccaa
     2221 catgatccca gcagtagaac gcgaaacctg catcagcgaa ttgttttgcc caggtctggt
     2281 agcgcatgga atgctcccca agcccatgaa caagtagtac tgcagcctta gggctggatg
     2341 gttgaatata ccatgtaaac agcctataac cgttagtttt taaccattcc tgatgaacag
     2401 attccacgtt gattagcgca gttgaatttt tcaaactgca atatagctaa aaacgtgaat
     2461 tgtttgcagg aagagttagt aaagttaaag cagaaaggga acaataataa ggaatgcttg
     2521 tgttgaagaa tgagatgtat gctggcaatt ttgagaggtt gaaaaccagc cttttacctg
     2581 tcaaaggaac tctccctgaa cttgtcgaag ggtggaatta gccagcagga tgagaagttt
     2641 attttatcct ttatcatttc tcatctgaaa tccgccggta ggcaaacaag ttcttgaatc
     2701 cgtcaggagc cggacaagtt cagccggta
//
LOCUS       DAOOQX010000227         2723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_45148_length_2723_cov_3.683658, whole genome shotgun sequence.
ACCESSION   DAOOQX010000227 DAOOQX010000000
VERSION     DAOOQX010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45148_length_2723_cov_3.683658"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1337)
                     /locus_tag="PLX53_04585"
     CDS             complement(66..1337)
                     /locus_tag="PLX53_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72389.1"
                     /translation="MRIAIIGFGAAAIGIMERLKESQHEIHVFEKSADIYSSSISGIR
                     ADGKLFVSSEMGGDITIDTALQRKLVDYYINFTEKKNVETGTSFGSSRFYKVFYDRGF
                     LPVTSEFFHIGTDQLKEVLFNIYTHLSGVKNIHFHFNSTVSSIKIEKEGVLLNGTEHF
                     DMAVVTVGRSGHKLINRLIEDYPYLVKNNTKVDLGIRFELPNHIVEELNHEMYEFKVK
                     YKSRTGYMVRTFCNNPSGFVVTENYDDFTTVNGHAKLHEKSSNTNFAILASLILTEPF
                     NDPIGYGSNIAKLSNLLAGEGKVILQTYSHFKESKRTKNLYRVSPTLAADKFILGDIN
                     LAYPRRIIESIIDFIENLNTVIPGVANNDNLVYAPEIKFYSNRLNNLANPFIKFAGDC
                     SGATRSIIYATAHGYMMGEAILNNNFSTLES"
     gene            complement(1364..>2723)
                     /gene="radA"
                     /locus_tag="PLX53_04590"
     CDS             complement(1364..>2723)
                     /gene="radA"
                     /locus_tag="PLX53_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOY72390.1"
                     /translation="GAESVKWLGRCPSCGEWNTYVEERITKETSSRPGLVNVSRNAIP
                     TPIREIDSDNELRVDTKIGELNRILGGGIVPGSVILLGGEPGIGKSTLALQLALGLKS
                     QKVLYISGEESARQVKLRADRINHDSQDCLILNETLLENILTQTERVCPELVVIDSIQ
                     TLYTDTIESSPGSVSQVRETAAALLRYAKTSGTPVILIGHITKDGSIAGPKVLEHIVD
                     VVLQFEGDQNYLYRILRSAKNRFGSTSEIGIFEMQSGGLVEVTNPSEILLSHREEMLS
                     GIAVAATIDGARPFLIETQALVSTAVYGTPQRSTTGFDPRRLNMLLAVLERRAGFRLA
                     NKDVFLNIAGGLRVNDPAIDLAIISAILSSTFDVAIAGSTCFAAEVGLSGEIRPVSRL
                     DQRIAEAAKLGMSRAFVSKYNLKKSDVHPKGIEVIPLGRIEELVKALFGRSSGRQQQA
                     DS"
BASE COUNT          630 a          678 c          680 g          735 t
ORIGIN      
        1 gaagttgata ccgttcacgg ccatgctttg cccaaaggca acgctcaggt tgtcaacctt
       61 cagcattaag attcgagggt tgagaagttg ttgttcagaa tggcctcacc catcatgtat
      121 ccatgagcag tggcgtaaat aatggatcgg gtagcgcccg agcagtcacc ggcaaacttt
      181 ataaatgggt tagccaggtt gttaagccgg ttggagtaaa atttaatttc gggcgcatac
      241 accaggttat cgttgttggc tactcccgga atgaccgtat tgagattttc tatgaagtca
      301 ataatgctct caattatccg tcgggggtag gccaggttga tgtcgcccag gataaactta
      361 tctgctgcca gcgtaggaga caccctgtag aggttttttg tgcgtttcga ctccttaaaa
      421 tggctgtagg tttgcagtat caccttacct tcgccggcaa gcaggttcga gagcttggca
      481 atgttcgatc cgtagcctat ggggtcgttg aatggttcgg ttaggatgag cgaggcgaga
      541 atggcgaagt tggtgttgct gctcttctcg tgcagtttgg catggccatt tacagttgtg
      601 aaatcgtcgt agttttcggt taccacaaac cccgagggat tgttgcaaaa agttcttacc
      661 atgtagcctg ttcggctctt gtacttcacc ttaaactcgt acatctcgtg atttagctcc
      721 tcaacaatgt ggttgggcag ctcaaaacgt atgcccaaat caaccttggt gttgttcttt
      781 accaggtagg ggtaatcctc aatcagcctg ttaatgagct tgtgaccgct tcggcccacc
      841 gttacaaccg ccatgtcgaa gtgttcggtt ccgttcagga gtacgccctc cttttcaatt
      901 ttaatgctgc ttaccgtgct gttgaagtgg aagtggatat tctttacccc gctaagatgg
      961 gtgtaaatgt tgaatagcac ctccttgagc tggtcggtgc caatatggaa aaactccgag
     1021 gtaacgggca aaaatcccct gtcatagaat actttataga atcttgacga gccaaatgaa
     1081 gtaccggttt caacattttt tttctcggta aagttgatgt agtaatcaac cagcttacgc
     1141 tgaagggcgg tgtcaatggt tatgtcgcct cccatttcgg atgagacgaa aagctttcca
     1201 tctgccctga tgcccgaaat gctagatgag tagatatcgg cgcttttctc aaacacgtgg
     1261 atttcgtgct ggctctcctt cagcctttcc ataatgccga tggcagcagc accaaacccg
     1321 ataatggcta tcctcatggc ttaaaaattt aatttcaaca tgtttagcta tcagcctgct
     1381 gctgacggcc tgacgatcgg ccaaaaagag ccttaaccag ctcttcaatt cgtcctaaag
     1441 gaataacctc tattcccttg gggtggacat cggatttctt aaggttatac tttgatacaa
     1501 aagccctgct catgcccagc ttggcagcct cggcaattct ctggtcgaga cggctaaccg
     1561 gccttatctc gcctgacagg cctacctcag cggcaaaaca ggttgaaccg gcaattgcca
     1621 catcgaaggt ggaggagaga atggccgata tgatggccag gtctatggca ggatcgttaa
     1681 ccctaagtcc accggcaatg ttcagaaaaa catccttgtt agcaagcctg aaacctgccc
     1741 ggcgctccag cacagccagc agcatgttca acctgcgggg gtcgaaacct gtggttgaac
     1801 gttggggggt tccgtaaact gccgtgctaa ccagggcctg ggtttcaatt aggaaaggcc
     1861 ttgccccgtc gatggtagct gcaaccgcaa tgccgctaag catttcctcg cggtgcgaca
     1921 gcaaaatttc cgatgggttg gtaacttcaa caagtccacc actctgcatt tcgaatattc
     1981 caatttcgga ggttgaccca aaacgatttt tggccgagcg gagaatgcgg taaaggtagt
     2041 tctggtcgcc ttcaaactgc agcaccacat ccacaatatg ctccagtacc ttagggccgg
     2101 caatgctccc atccttggtg atgtggccta taaggataac cggagtcccc gatgtttttg
     2161 catatctgag cagggcagcc gccgtttcac gcacctgcga aacactgcct ggcgacgatt
     2221 caatggtatc ggtgtagagt gtttgaatgg agtctattac caccaactcg gggcataccc
     2281 tttcggtttg ggtgagtata ttttccagca gcgtttcgtt gagtataagg cagtcctggc
     2341 tgtcgtgatt aatacggtcg gcgcgtagct taacctggcg ggcgctttcc tcccccgata
     2401 tgtagagcac tttttgactt tttaggccta gtgccagctg aagggcgagt gttgattttc
     2461 caataccagg ctccccgccc agcagaataa ccgaaccggg tacaattccc ccgcccagta
     2521 tgcggtttag ctctccaatt ttggtgtcaa cccgtaactc gttgtccgaa tcaatttccc
     2581 tgatgggagt agggatagcg ttccgtgata cgtttaccag ccctggcctg ctggaggttt
     2641 ccttggtaat tctttcctcc acataggtat tccactctcc acatgagggg cagcggccaa
     2701 gccacttcac cgattctgcc ccg
//
LOCUS       DAOOQX010000228         2719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_45234_length_2719_cov_3.129505, whole genome shotgun sequence.
ACCESSION   DAOOQX010000228 DAOOQX010000000
VERSION     DAOOQX010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45234_length_2719_cov_3.129505"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLX53_04595"
     CDS             complement(<1..213)
                     /locus_tag="PLX53_04595"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HOY72391.1"
                     /translation="MMGGLALQDFLVVSTSMFFIGIFGFLTRRNLITILMSVELILNS
                     VNINFVAFNYFLHPEAMHGIFFAIFVI"
     gene            complement(215..769)
                     /locus_tag="PLX53_04600"
     CDS             complement(215..769)
                     /locus_tag="PLX53_04600"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOY72392.1"
                     /translation="MEIAASKVIFYIIAAAILFFALLSVTSKRILRAATYLFIVLIST
                     AALYFLLNYQFLGAVQIALYAGGIVVMIIFSILLTSHAGQKFEPVSRLKFFASLLAVL
                     VGWALSLWVILSHPFVASSKEAVEVNMDSIGKALLSYDRNGYVLVFEVISILLLAAMI
                     GAIVISKKQPGDGETNPDNSKTSK"
     gene            complement(772..1248)
                     /locus_tag="PLX53_04605"
     CDS             complement(772..1248)
                     /locus_tag="PLX53_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOY72393.1"
                     /translation="MGLKSYLSGLFKGFYSLLVGLRVTNSYFFHPSRIVTQQYPENRK
                     TLKMFERFKGEVILVHNENNEHRCTGCGICEMNCPNGSIEIISKMETGEDGKKRKAID
                     KHIYHLSMCTFCGLCVKSCPSKALAFGNEFEHAVFDRAKLDKVLNKEGSVLMKGVE"
     gene            complement(1260..2282)
                     /gene="nuoH"
                     /locus_tag="PLX53_04610"
     CDS             complement(1260..2282)
                     /gene="nuoH"
                     /locus_tag="PLX53_04610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOY72394.1"
                     /translation="MSDLWATVLEYAIIGIIYLVIYALIGLFLVYAERKVCAFIQVRL
                     GPNRVGPKGFFQTIADFIKLLLKENIKIKNADGVLFNLAPFIVIVASFLTFGTVPFAK
                     GLQGIDLNIGVFFIMAVSSMGAVGILLGGWSSNSKYSLIGAMRSGAQIVSYELSVGLS
                     LVTMVILTGTMQLSQIIELQSDGWFIFKGHIPALISLIIMMIASTAEINRGPFDLAEA
                     ESELTAGYHTEYSGIKFAFFYLSEYMNMFVVSALVATVFFGGWMPLHIGSWQAFNHIM
                     DFIPPIIWFILKSFALMYVIMLFKWTFPRLRIDQLLKLEWQYLLPISLFNVLLMAFVV
                     YMKWYF"
     gene            complement(2395..>2719)
                     /locus_tag="PLX53_04615"
     CDS             complement(2395..>2719)
                     /locus_tag="PLX53_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOY72395.1"
                     /translation="VRMDEMLESLKIIEQLIDNIPEGSYVEKMKAVIKLPEGEYFQQV
                     ETARGELVVHIISDGNKTPYRVKFRSPCLSTLSVMDLISRNQKIADLIAIGGSLDLVI
                     PDIDR"
BASE COUNT          815 a          649 c          577 g          678 t
ORIGIN      
        1 aataacaaat atggcaaaga agattccgtg catggcctcg gggtgcagaa agtagttgaa
       61 ggccacaaag ttgatgttta ccgagttaag tataagctca acggacatta aaatggtaat
      121 caggttgcgt cgggtaagaa agccaaaaat tccaataaag aacatggatg tgctgacaac
      181 cagaaaatct tgtaaagcta aacctcccat catatcattt gcttgtttta gagttatcgg
      241 gattggtttc cccatcaccg ggttgttttt ttgaaataac aatcgcccca atcattgcag
      301 ccagcaggag aatagaaata acctcaaata ccaaaacata cccgtttcta tcatagctaa
      361 gcaaggcttt cccgattgag tccatgttca cttcaaccgc ttcctttgaa cttgccacaa
      421 aagggtggct cagaattacc cagagcgaca aggcccatcc taccaaaacg gccaacaacg
      481 acgcaaaaaa tttaagacgg cttaccggct cgaatttctg accggcatgg ctggtcaaaa
      541 gaatggagaa aataatcatc accacaatac caccggcata taaagcaatc tgcacggctc
      601 ccagaaactg gtagttcagc agaaagtaca gagctgctgt ggatatcagt acgataaata
      661 ggtaggtggc agcccgcaga atccgcttgc tggtaaccga gagcagcgca aaaaaaagta
      721 tggccgcagc aatgatgtag aatattactt ttgaggcagc tatttccatg gctattcaac
      781 tcctttcatt aaaacagaac cttccttatt cagaaccttg tcgagctttg ccctgtcgaa
      841 aaccgcatgt tcaaattcgt tgccaaaggc aagcgccttt gatggacagc tcttaacgca
      901 caagccgcaa aaggtgcaca tgctcaggtg gtaaatgtgc ttgtcgatcg cctttcgctt
      961 tttcccatcc tcacccgttt ccattttcga tatgatttcg atggagccgt tggggcagtt
     1021 catctcacat attccgcaac cggtgcatct atgctcatta ttctcgttgt gcacaagtat
     1081 aacctcaccc ttaaagcgct cgaacatttt aagagtcttc ctgttctcgg ggtactgctg
     1141 ggttacaatt ctgctggggt gaaaaaagta ggagttggta acccgcaggc ctactaaaag
     1201 cgaatagaat cctttgaata atcccgagag gtatgatttt aatcccataa tttacaagtt
     1261 taaaagtacc atttcatgta caccacaaac gccatcagca gaacgttgaa caggctaatc
     1321 gggagcaggt actgccattc gagcttaagc agctgatcaa ttctaagcct tgggaatgtc
     1381 cacttaaaca gcataataac atacattagg gcgaatgatt tcagtataaa ccagatgatg
     1441 ggtggtataa agtccattat atggttaaac gcctgccatg agccgatgtg tagtggcatc
     1501 catcctccaa aaaataccgt ggcaactagc gccgacacaa caaacatgtt catgtattca
     1561 gagaggtaaa agaaggcaaa tttaatgccc gagtactcgg tgtggtaacc ggcggtaagc
     1621 tccgattcgg cttcagccag gtcgaaaggc cccctgttga tttcggcggt gctggcaatc
     1681 atcatgatta ttaaggatat tagggcgggt atgtggccct taaatataaa ccacccatcg
     1741 ctttgcagct caataatctg tgatagctgc attgttccgg tcaggataac catggtaacc
     1801 agcgagagcc ccaccgatag ctcgtagcta acaatttgag ccccgcttcg catggcgcca
     1861 atcagcgagt acttactatt gcttgaccat ccacccagca aaattcccac cgcacccatc
     1921 gaagaaaccg ccataataaa aaacacccca atgttcaggt caattccctg cagccctttt
     1981 gcaaagggaa ctgtgccaaa tgtgaggaac gatgcaacaa taacaatgaa aggcgccagg
     2041 ttaaaaagca ccccatcagc attttttatc ttgatattct ccttaagaag gagcttgatg
     2101 aagtcggcta tggtttggaa aaatccctta gggccaaccc tgttgggacc caggcgaacc
     2161 tgaatgaagg cgcaaacctt acgctcggcg taaaccaaaa atagcccgat aagcgcataa
     2221 ataaccaggt agatgatgcc gataatggca tattccaaca cagtagccca caaatcggac
     2281 atgcttgctc gaaataccgc atcaatactc tgtattaacc cagtaaaatc atataaaaca
     2341 ccctgcatag tattagcgtt tagtgctgaa tggttaatgt ttgcccataa gcccttacct
     2401 gtcgatatca gggattacca aatccaatga tccgccaatg gcaattaggt ctgcaatttt
     2461 ttgatttctt gaaatgaggt ccataaccga aagggttgaa aggcaaggtg aacggaactt
     2521 cacccggtag ggtgttttgt tgccatcgct gataatgtgc acaacgagct cgccacgggc
     2581 tgtttcaacc tgctgaaagt actcgccttc gggaagcttt ataacagcct tcattttttc
     2641 cacgtagctg ccctcgggga tattatcaat aagctgctcg atgatcttca acgattccag
     2701 catttcatcc attctaacc
//
LOCUS       DAOOQX010000229         2711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_45414_length_2711_cov_3.301581, whole genome shotgun sequence.
ACCESSION   DAOOQX010000229 DAOOQX010000000
VERSION     DAOOQX010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45414_length_2711_cov_3.301581"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /gene="rpsB"
                     /locus_tag="PLX53_04620"
     CDS             <1..708
                     /gene="rpsB"
                     /locus_tag="PLX53_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOY72396.1"
                     /translation="AAVKQIAKSGRKILFVATKKQAKDIVAERIKPINMPYVTERWPG
                     GMLTNFATIRKAVKKMSSIDKMMTDGTIDTLSKREKLQVSRQRAKLEKNLGSIADLNR
                     LPAALFVVDVQKEYIAVREAKRLNIPVIAMVDTCCDPTPIDFVIPANDDASKSIALIV
                     DIMAKAIEEGLEERKAEKEKEPAQKEKSEEQPTRTRARKVKVKVESTHNEGNAEVADS
                     VEEVAEQNTSVEENEQE"
     gene            732..1559
                     /gene="tsf"
                     /locus_tag="PLX53_04625"
     CDS             732..1559
                     /gene="tsf"
                     /locus_tag="PLX53_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOY72397.1"
                     /translation="MPNITAADVSKLRKITGAGMMDCKNALVEAEGDIDRAIELIRER
                     GKAIATKRAGREAGEGAGLAKVNANATRGAMIVLNCETDFVAKNEDFLKVAKKILDFA
                     LDTAPSNLEALKESVMEGRKVSDMITEFSGITGEKVELSYYDQVEAPMVVPYIHPGNK
                     LATIVGLSKVTDMQVAKDIAMQVAAMNPVSIDKEDVPEEVKLKELEIGREQARLEGKP
                     ENMLDKIAEGKLQKFFKESTLLNQEFIKDNKMTVRQYIQSADKDLKVLAFKRVSLNV"
     gene            1681..2391
                     /gene="pyrH"
                     /locus_tag="PLX53_04630"
     CDS             1681..2391
                     /gene="pyrH"
                     /locus_tag="PLX53_04630"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOY72398.1"
                     /translation="MIKYKRILLKLSGEALMGADAYGISTDVLNSYARQIKEVTDLGV
                     EVGIVIGGGNIFRGLKGCSMGFDRVKGDQMGMLATVINSLALQSAIEGIGGKARVLTA
                     TKMEPVGELYSKDKAIQVLSEGNACIIAGGTGNPFFTTDTASALRAVEINANILLKGT
                     RVDGVYSADPEKDPSAQRFESITFTEAYDKKLKIMDLTAFTLCSENNLPILVFDMNTP
                     GNLKKAVFGDKVGTLVKN"
     gene            2423..>2711
                     /locus_tag="PLX53_04635"
     CDS             2423..>2711
                     /locus_tag="PLX53_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOY72399.1"
                     /translation="MNPNDATATISTADEHMQKAIDHLENELRVIRAGRANPAILSGV
                     MVDYYGTMTPLSQVSSVGTPDARTIVVQPWEKNMINPIEKAIMAANLGFNPQ"
BASE COUNT          846 a          604 c          650 g          611 t
ORIGIN      
        1 gctgcagtaa agcaaattgc caaatcggga cgcaaaatcc tctttgttgc taccaagaag
       61 caggccaagg atattgtggc cgagcgaatt aaaccaataa acatgcccta tgttacggag
      121 cgctggccag gcggaatgct caccaacttt gccactatcc gtaaggccgt taaaaaaatg
      181 tcctccatcg acaagatgat gaccgacggc accattgata ccctatcaaa acgtgaaaag
      241 cttcaggttt cgcgtcagcg agccaagctg gaaaagaacc tcggctcaat tgccgacctc
      301 aacaggctgc cagcagccct ttttgtagtg gatgttcaga aggaatacat cgctgttcgt
      361 gaagccaagc ggcttaacat accggtaatt gcaatggttg atacctgttg tgatcctact
      421 cccattgact ttgtaattcc ggctaacgac gatgcttcca aatccattgc cctgatagtt
      481 gacattatgg ccaaagctat tgaggaaggg cttgaggaaa gaaaggctga aaaggaaaaa
      541 gagccagccc aaaaggaaaa gagcgaagaa cagcccaccc gaacccgtgc ccgcaaggta
      601 aaggttaagg ttgaatcaac ccacaatgag gggaatgctg aagttgccga ttctgttgag
      661 gaggtagccg aacaaaacac aagcgttgaa gaaaacgagc aagagtagta aattctaaaa
      721 agggaaaata tatgccaaat ataactgcag cagatgtctc caaactacga aagataaccg
      781 gagcaggaat gatggattgc aagaatgccc tggttgaagc tgaaggagat attgataggg
      841 ctattgagct tattagggaa cggggaaagg caattgccac caaaagagca ggcagagagg
      901 caggtgaggg tgctggttta gcaaaagtga atgccaacgc tacccgtggt gcaatgattg
      961 tgctcaactg cgaaaccgac tttgtggcta aaaatgaaga cttccttaag gtagccaaaa
     1021 aaattctaga ctttgcgctt gatacagctc catcgaacct cgaagcgctt aaggaatctg
     1081 ttatggaagg ccgaaaggtt tccgatatga ttaccgaatt ctccggaatt accggggaaa
     1141 aagtggagct ctcctactac gatcaggttg aagcccccat ggtagttccc tacattcacc
     1201 cgggcaataa gctggccacc atagtcggcc ttagcaaggt taccgacatg caggttgcca
     1261 aggacatagc catgcaggtt gccgctatga accctgtttc aattgacaag gaggatgttc
     1321 ccgaagaggt caagctgaaa gaacttgaaa ttggccgcga gcaggcacgc cttgaaggaa
     1381 aacccgaaaa catgctcgac aagattgccg agggtaaact acagaaattt ttcaaggaaa
     1441 gcaccctgct gaaccaggag ttcatcaagg ataacaagat gaccgttcgc cagtacatcc
     1501 agagcgccga taaggatttg aaagttctgg cctttaaacg ggtatcgctc aatgtttagc
     1561 acaacatcaa agcattaaaa cctcgtgtta taccacgggg tttttttatg ttaccctgac
     1621 ccttacgggt aaaattcttt ttttgtacct ttacatcatc atcccgataa tactatcaaa
     1681 atgattaagt ataaaagaat tctgcttaag ctcagtggtg aagcccttat gggtgctgac
     1741 gcctatggga taagcaccga cgtacttaac tcgtatgcgc gtcagatcaa ggaggtaacc
     1801 gatttgggtg tagaagttgg aatagttata ggtggtggaa atatttttcg tgggcttaag
     1861 ggatgttcaa tgggtttcga ccgggtgaaa ggagaccaga tgggcatgtt ggccaccgtt
     1921 attaacagcc tggcacttca atcggccata gaaggtattg gtggaaaagc aagagtgctc
     1981 accgctacca aaatggaacc cgtaggtgag ctctactcaa aagacaaagc catacaagtg
     2041 ctttccgaag ggaatgcctg cattattgcc ggaggaacag gaaacccctt ttttactacc
     2101 gacaccgctt cggcacttcg ggcagttgaa atcaacgcta acattctttt aaagggaacc
     2161 cgggtggatg gcgtttactc ggccgacccc gaaaaagatc cctctgccca gcggttcgag
     2221 agcataactt ttactgaagc atacgataaa aagctaaaaa ttatggattt gaccgccttt
     2281 accctctgta gcgaaaacaa cctccccatc ctggtttttg acatgaacac ccctggaaac
     2341 ttaaaaaaag ctgtctttgg cgataaggtt ggaactttag ttaaaaatta aatactttta
     2401 caataaaatt aacagtaagt gtatgaaccc caacgatgca acagcaacca tcagcactgc
     2461 tgatgaacat atgcaaaaag ccattgatca ccttgaaaac gagttacggg taatacgcgc
     2521 cggcagggct aaccccgcaa tactttctgg agttatggta gactactacg gaacaatgac
     2581 gcccctatct caggtgtcga gtgtaggaac ccccgatgcg agaacaattg tagtgcaacc
     2641 atgggaaaaa aacatgatta acccaataga aaaggctatc atggcagcca acctggggtt
     2701 taacccccag a
//
LOCUS       DAOOQX010000230         2698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_45701_length_2698_cov_3.580401, whole genome shotgun sequence.
ACCESSION   DAOOQX010000230 DAOOQX010000000
VERSION     DAOOQX010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45701_length_2698_cov_3.580401"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PLX53_04640"
     CDS             <1..536
                     /locus_tag="PLX53_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOY72400.1"
                     /translation="NVFDDFIAAAEYLIKEKYTSPKKLVIQGGSNGGLLIGAVINQRP
                     DLFKVAIPEVGVMDMLRFHKFTIGWAWVNDYGSSEDSIQFQNLYSYSPLHNIKPNTKY
                     PAILVTTADHDDRVVPAHSFKYIATMQEKNPNPQNPALIRIQTRAGHGAGTPTSLIID
                     EYTDIYAFILYNLGLSI"
     gene            711..1340
                     /locus_tag="PLX53_04645"
     CDS             711..1340
                     /locus_tag="PLX53_04645"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOY72401.1"
                     /translation="MQYLVIEGNIGAGKTTLATMLANDLNANLITEQFADNPFLPKFY
                     ENPERYSFALELSFLAERYRQLNAELRMGSLFHSLTIADYYFMKSLIFAQNTLSGDEL
                     QLYKQIFQIIYNTLPKPNLYVYLHLPVQMLLRNIKKRGREYEQSITGEYLESIAQGYF
                     DFFKQHPDYTFLIIETSKLDFVANQHDFNKIKSAIFDKTYPNGVNRLTF"
     gene            1451..>2698
                     /locus_tag="PLX53_04650"
     CDS             1451..>2698
                     /locus_tag="PLX53_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72402.1"
                     /translation="MMKGLKFAIVALAGVVLAGCGGAEKMKELPQGTSFKVTPNPLEV
                     HANKVKATIEGTVPAKYFNKKATLEITPVLVYNGGELALPSKILQGEDVQANNQVINY
                     EAGGSWKHDVEFDYKDEMFRAEMELRFVITYKDKQIPFDAPQKIGIGTMATYKLVDLD
                     PKPVMLKDKFVRVTPEEKEAQINFVIQKSDIRKSELTKDEVKMLNDFLLDAAKTSNKQ
                     LKEIKVSAYASPDGPETLNDKLSKERGKNTDSYLADVFKKGKVTNPATVSVETTSEDW
                     EGFQKQVQASNIEDKDLILRVLSMYSDPEVREKEIKNLSKVYKVLAEKVLPELRRSRM
                     IAKVDKVGYSDDTLKYMVENNAYDSLNVEEMLYAATLVDNVDKKIEVYMKAGEKFNCA
                     RGYNNAAYYYILNKNTAAAKAALD"
BASE COUNT          856 a          584 c          557 g          701 t
ORIGIN      
        1 aaaatgtgtt tgacgacttt atagcagctg ctgaatacct tattaaggaa aagtatacct
       61 cacccaaaaa gctggtcata cagggtgggt caaatggtgg cctcctgata ggagcggtta
      121 ttaaccagcg tcccgattta tttaaagtag ccatcccgga agttggagtg atggatatgc
      181 tgcgttttca caaatttacc ataggctggg catgggttaa cgactatgga tcaagcgagg
      241 attccattca gtttcagaat ctctacagct actccccgct gcacaacatt aaaccaaaca
      301 caaaataccc agccatattg gtaacaacgg ccgaccatga cgacagggta gttccagcgc
      361 actcgttcaa gtacattgcc accatgcagg aaaaaaatcc caacccgcag aatcctgcac
      421 ttatcagaat tcaaacccgt gcaggccatg gtgctggaac accaacatcc ctgatcattg
      481 acgagtacac cgacatttac gcttttatac tttacaacct ggggctatcc atctagctct
      541 tttatgaatg aatacttttt taaccatggg ggttgagcaa cagcttatcc cccattcata
      601 ttgctgtaca acaacggtaa attccgaaac atagtgacaa ttattgatga cgaattgcgg
      661 aacagaatat gctaacttag ccaagtagtt ataaataagc aagtaggaat atgcaatacc
      721 ttgtaataga agggaatatt ggtgcaggga aaactaccct ggccaccatg ttggcaaacg
      781 atttaaatgc caatctcatt acagagcaat ttgccgataa cccattttta cctaaatttt
      841 acgaaaatcc tgaaaggtat agctttgctc tggagctatc gtttctggcc gaacgctaca
      901 ggcagctgaa tgccgagcta cgaatgggtt cattattcca tagtcttacc attgccgact
      961 actactttat gaagtcgctc atttttgccc agaacaccct ttcgggtgat gaacttcaac
     1021 tctacaagca aattttccaa atcatataca acacccttcc caagccaaat ctttacgttt
     1081 acctgcatct tcccgttcag atgctactac gaaatataaa aaagcgcggc agagagtatg
     1141 agcaatccat taccggcgag tacctcgaaa gcatagcaca ggggtacttc gattttttca
     1201 agcagcaccc tgactacacc tttctaatca tcgaaacctc aaagttggat tttgttgcaa
     1261 atcagcacga ttttaataaa attaaaagtg caatttttga caaaacctat ccaaatggtg
     1321 ttaaccggct tacattttag gataaatgta tttttagggc atatttttta tttgtaaaaa
     1381 aaaatttcta agtttgtaga tgatttactg aaaaaatcga tgttattaac tattaaccaa
     1441 atttgataca atgatgaaag gattaaaatt tgcaatcgta gcactggcag gagttgttct
     1501 ggcaggatgc ggcggtgccg aaaagatgaa agaattaccc cagggcacca gctttaaggt
     1561 taccccaaac ccacttgagg ttcacgccaa caaggtaaag gccactattg agggtacagt
     1621 tcccgcaaag tatttcaaca aaaaagccac actggaaatt acccctgtac tagtttataa
     1681 cggtggtgag ctggctcttc cttccaaaat tcttcagggg gaagatgtgc aggccaacaa
     1741 ccaggtaatt aactacgaag caggcggttc atggaagcat gatgttgaat ttgactacaa
     1801 ggacgaaatg tttcgtgccg aaatggagct gcgctttgta atcacctaca aggacaagca
     1861 gattcctttt gatgctcctc agaaaattgg tattggaacc atggcaacct acaagctggt
     1921 tgacctagac cccaagccag ttatgctgaa ggataaattt gtaagggtta cccccgagga
     1981 aaaagaagct cagatcaact tcgtaatcca aaagtcggat attcgcaaat ccgaacttac
     2041 caaggatgag gttaaaatgc taaacgattt tttactcgat gctgccaaaa ctagcaacaa
     2101 gcagcttaaa gagattaaag tatcagccta tgcttcgccc gatggaccag aaactcttaa
     2161 cgataagctt tcgaaggaac gtgggaagaa caccgattca tacctcgccg atgtgttcaa
     2221 aaagggtaag gtaacaaatc ccgctacggt aagcgttgaa accacttctg aagactggga
     2281 agggttccag aagcaagttc aggcctcgaa cattgaagat aaggacttaa ttcttcgagt
     2341 tctttccatg tactccgatc ctgaggttcg cgagaaagaa attaaaaatc tatcgaaggt
     2401 ttacaaggtg ctggccgaaa aggtactccc tgaacttcgt cgttcgagaa tgattgccaa
     2461 ggttgacaag gtaggctact ctgacgatac gttaaagtat atggttgaga acaatgctta
     2521 cgattcactt aatgttgagg aaatgcttta cgcagcaacc cttgtagata acgttgataa
     2581 aaagattgag gtttacatga aggcaggcga aaagttcaac tgcgctcgcg gttataacaa
     2641 tgctgcatac tactatatcc tcaacaagaa cactgctgct gcaaaggctg ccctggat
//
LOCUS       DAOOQX010000231         2696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_45766_length_2696_cov_2.823930, whole genome shotgun sequence.
ACCESSION   DAOOQX010000231 DAOOQX010000000
VERSION     DAOOQX010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45766_length_2696_cov_2.823930"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(351..650)
                     /locus_tag="PLX53_04655"
     CDS             complement(351..650)
                     /locus_tag="PLX53_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72403.1"
                     /translation="MKRVELDFLFILPFIPVLLPAQVTTDDYMRAEKFLQANLGKHIY
                     NSWVEPSWQKDGCTFVYSVNTRKGKEFFRVDAKVGVKIPAFNHAKLASLSPQANR"
     gene            complement(700..>2696)
                     /locus_tag="PLX53_04660"
     CDS             complement(700..>2696)
                     /locus_tag="PLX53_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOY72404.1"
                     /translation="TLRDMIFSSVSEEWRKAYDAGVFTEFQEQRAPGHTVAGKKVFQK
                     GMLDLIDDVDVSLNKLTKDNNGCTKAKIEELEAMRIAANAIIKFANRYADELERLAKI
                     EVDANRRVELGEMAAICRRVPAHAPQTFHEALQHYWFIHLGVITELNPWDSFNPGRLD
                     QHLYPFYLNDIKEGKLTHEGAVELLQAFWVKFNNHPSPPKMGVTAEESNTYTDFTLVN
                     LGGVKEDGSDAVNELTYTILDVIEEMRILQPSSMVQVSKKNPDRFVKRAVKIVKTGFG
                     QPSFFNSDAIVQELLSQGKSLTDARNGGASGCVETGAFGTEAYILTGYFNLNKVLELT
                     LNNGVDPLTGKEIGIRTGNAASFKTFNQLYEAFERQLGYFSDVKLEGNLVIERIWAEE
                     MPVPFLSLLIDDCIANATDYNAGGARYNTSYVQGVGLGSITDNLSALSRLVFDEEALS
                     MDELLKAMNANFEGYDNLRYRLIYDMPKWGNNNDYADKLAVRVFNSFYKAITGRKTFR
                     GGVFRINMLPTTSHVYFGSKIGAMPDGRLAHEPLSEGISPVQGADRNGPTAVILSAAK
                     IDQIKTGGTLLNQKFSPSFFTTDESLDKMVSLIRSYFKLDGHHIQFNVVSADTLRDAQ
                     KHPEKYQDLIVRVAGYSDYFNDLGEALQNEIIRRTEHEAV"
BASE COUNT          626 a          684 c          627 g          759 t
ORIGIN      
        1 caaacgatga atgtaattgg tagtaccgtc aagagggtct ataatccagg tatattccct
       61 cgctcggcga agtctgcgac tttgtcgtac ctaagtagcg gtctctgacc gcgtaaaatc
      121 ctatcagtag gttttccatt ggggggaaat atacgcaatt gtaccctcgc tgccgcacct
      181 ctcccgtcgt gtggcaaccc catccctagt tctctgccgc aacaactacc cccttatcct
      241 taaatttata gcgcataatc tcgctggtat tgttttacaa cttgctacca ctcactttcc
      301 atttcttttc ttaactgcca cacggttggc ttcgccgagt tatccgccag ctagcggttc
      361 gcttggggtg atagcgacgc tagcttggca tggttgaatg ccggaatctt taccccgact
      421 tttgcatcaa ccctaaaaaa ctccttccct ttccgggtgt tcacgctata cacaaaagtg
      481 cagccatcct tctgccacga cggttccacc cagctgttgt agatatgttt tccgaggttt
      541 gcctgaagaa acttctcagc ccgcatgtag tcgtccgtgg ttacctgtgc cggtaatagt
      601 accggaatga atggcagtat gaataagaaa tcaagttcta ctcttttcat gatttacagt
      661 gtttattgat tttggcatag cccatcaaca cgtttgccgt taaacggcct catgctcggt
      721 tcggcggata atttcattct gaagggcttc gcccaaatcg ttgaagtagt cggagtaacc
      781 ggcaaccctt acaattagat cctgatactt ttcggggtgc ttttgagcgt cgcgcagcgt
      841 atcggcgcta accacgttaa actgaatgtg gtgaccgtcg agtttaaagt agctccgtat
      901 gagcgatacc atcttgtcga ggctttcgtc ggtagtgaaa aacgatggcg aaaacttttg
      961 gttaagcagc gttcctccgg ttttgatttg gtcaatctta gctgccgaaa gaataacagc
     1021 agttggtccg tttctgtctg ctccctgcac ggggctaatt ccctcgctga gaggttcatg
     1081 agccaagcgt ccatcgggca ttgccccaat tttgctgcca aagtaaacat ggctggtggt
     1141 agggagcatg tttattcgga aaaccccgcc cctgaaagtt ttccgtccgg taatggcctt
     1201 gtagaagctg ttgaaaaccc taacggccaa cttgtcggca taatcgttgt tgttgcccca
     1261 tttgggcata tcgtaaatca gtctgtagcg aaggttgtcg tagccctcaa agttggcatt
     1321 catggcctta agcagctcat ccatagataa ggcttcctca tcgaacacca gcctgctcag
     1381 agccgacagg ttatcggtta tgctacccag cccaaccccc tgcacgtagc tggtgttata
     1441 ccttgcccca ccggcattat agtcggttgc gttggcaata cagtcgtcta ttaggagcga
     1501 gaggaatgga acaggcattt cctctgccca aatacgctca atgacaaggt tcccctcaag
     1561 ctttacatcg gaaaagtaac cgagctgcct ttcaaaagcc tcgtagagct gattgaaagt
     1621 tttgaatgaa gcggcattgc cggttcttat tccgatttcc ttacctgtta gcgggtcaac
     1681 accattgttc agggttagct cgagcacctt gttcaggttg aagtagcctg ttagaatgta
     1741 ggcttcggtt ccaaaggctc ctgtttcaac acaaccgctt gctccgccgt ttcgagcatc
     1801 agtaagcgat ttgccctggc ttagcagttc ctgcacaatg gcatcggagt taaaaaacga
     1861 tggctgtcca aaaccggtct taactatttt gactgcacgc ttcacaaacc tgtcggggtt
     1921 tttcttgcta acctgcacca tgctgctggg ttgtagtatt cgcatctcct caataacatc
     1981 aaggatggtg taggtgagct cgttaaccgc atcggagccg tcctccttaa caccaccaag
     2041 gttaaccagg gtaaaatcgg tgtaggtgtt gctttcctca gctgttacac ccatcttggg
     2101 cggtgaagga tggttgttga acttcaccca gaaggcttgc agcagctcaa cagcaccttc
     2161 atgggtaagc ttgccctctt tgatatcgtt caggtagaaa gggtagagat gctggtcaag
     2221 gcgtccgggg ttgaaggagt cccaaggatt taactcggta attacaccaa gatgaataaa
     2281 ccagtagtgc tgcagcgcct cgtgaaatgt ttgaggggca tgggcaggca cccttcggca
     2341 aatggcggcc atttcaccaa gctctactcg cctgttggca tcaacttcga tttttgccaa
     2401 cctttccagc tcatcggcat aacgatttgc aaattttatg atagcattag cagcaatccg
     2461 catggcttcc agttcttcta tctttgcctt tgtgcagcca ttgttatctt tggtaagctt
     2521 gtttagtgaa acatccacat cgtcaattaa atcgagcatt cccttttgga aaactttttt
     2581 accggcaaca gtgtggccgg gagctcgctg ttcctgaaac tcggtgaaaa cgcctgcatc
     2641 gtatgcctta cgccattctt cactaactga cgaaaaaatc atatcgcgca gggttt
//
LOCUS       DAOOQX010000232         2681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_46084_length_2681_cov_3.543031, whole genome shotgun sequence.
ACCESSION   DAOOQX010000232 DAOOQX010000000
VERSION     DAOOQX010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46084_length_2681_cov_3.543031"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="PLX53_04665"
     CDS             <1..1310
                     /locus_tag="PLX53_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOY72405.1"
                     /translation="FAVGGSSGFETIPWTDPIFMEILVKRSDESSFSSMGTTQIISVP
                     YALVANNGIQGPYIQGQTVFSNGGGWVGTNRVMVADSSVVVTTPLGHDSSKPIFAVTN
                     ANGEMVMAVYESGVRFYVEEETTKGVKGGFAVGGLTNQVKETKSGEPIYYMNIQPTQV
                     QFLVEEPAVKGVKGGFAVGGLTNQGKTLTPTENYFEVNRDSAQFLTPSLVSQGNITTQ
                     GNITVSGNITTNVGQADSTLTDVDGNTYQTVRIGFQQWMAENLKTTHYSDGTPIDTAV
                     HVYNDWAYPDSINIYGRLYSGLAISSGNNVCPDGWHVPTPDDWNELFTFIAGPAGISD
                     MQSRVLVLKKLLEPGTMMEGNGYWNYPIYPTNETGFTARPAGQRDYLTYYSELHDYAY
                     FWSYVVGVGLQQVSMTTMDGGNLEIMPPSDSNYSFSVRCLQNP"
     gene            1742..>2681
                     /locus_tag="PLX53_04670"
     CDS             1742..>2681
                     /locus_tag="PLX53_04670"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY72406.1"
                     /translation="MNKKKVIIAGGGFAGIQLARKLDQRLFDVLLVDKINHHQFQPLF
                     YQVATSQLEPSSISFPLRNIFSRKKNVQIRLAEILSIDRNESKIITSIGEFSYDYLVL
                     AMGCTNNFFGNENIKKNAFTLKTTYDAITIRNHIILTFEKIVSAKEDELEALNNLAIV
                     GAGPTGVELAGAFAEIKKNILPKDYAGVDFSKFNIILIEGTRNTLGSMSDASKVESRR
                     YLQQMGVTILTETFVADYDGYLLKLSNGETIKTKTVIWVAGVTGNNIEGLPEAARAPG
                     NRISVDRFNRVNGCDNVFAVGDIAYMVTPKYPKGHPQ"
BASE COUNT          790 a          640 c          605 g          646 t
ORIGIN      
        1 cttttgctgt tggcggtagc agcggatttg aaaccattcc ttggaccgat ccaatattca
       61 tggagatcct tgtaaagaga tcggacgaat ccagcttttc ctccatggga actacccaga
      121 ttatctcggt tccctatgcc cttgttgcca acaacggtat tcaggggcct tacattcagg
      181 gtcaaaccgt tttcagcaat ggcgggggtt gggtcggcac taaccgagtg atggtggccg
      241 acagctcggt ggttgttact acaccccttg gccacgactc atcaaagcca atttttgccg
      301 ttaccaacgc caatggagaa atggtgatgg cggtttacga atcgggcgtg aggttttatg
      361 ttgaagagga aactaccaag ggagtaaagg gtggatttgc cgtgggcggt ctcaccaacc
      421 aggtgaaaga aacaaaaagc ggtgagccca tttactacat gaacattcag ccaacacagg
      481 ttcagttcct ggttgaggaa ccagctgtca agggagtaaa gggtggattt gccgtgggcg
      541 gtctcaccaa ccagggaaag acgttaactc ctactgaaaa ctactttgag gttaacaggg
      601 actccgccca attccttact ccatcgctgg tatctcaggg taacataacc actcagggta
      661 acattactgt tagcggaaac attaccacca acgttggaca agctgattcc acactgaccg
      721 atgttgacgg caacacctat cagacagttc gcattggatt ccagcagtgg atggccgaaa
      781 atcttaaaac aacgcactat tctgatggaa cgccgattga caccgccgtc catgtctata
      841 acgactgggc ttaccccgac tccatcaaca tatacggcag gctctacagc ggactggcta
      901 ttagcagcgg taataacgtt tgccccgatg gttggcatgt tccaactcct gacgactgga
      961 atgagctgtt tacattcatt gccggtcctg cagggatttc ggatatgcaa tccagggttc
     1021 ttgttctcaa aaaattgtta gagccaggca ccatgatgga aggaaacggc tactggaatt
     1081 accccatata ccccaccaac gaaacggggt tcactgccag gcctgcagga caaagagact
     1141 acctgaccta ctattccgaa ctgcacgact atgcctactt ctggtcatac gttgtgggtg
     1201 ttgggttgca acaggtttcc atgacaacaa tggatggagg taatctagaa attatgccac
     1261 catcagattc taattactca ttctcagtta gatgcttgca aaatccttaa aaagagaatt
     1321 tttagcaaca ggctagctct tctaacttag aacaacttgc gctctataaa gtatagctaa
     1381 cttgctgctc agctggcaaa gatcaagggt cggagcaagg tttactccga cccttatttt
     1441 tcgccctgct aaattttcgc cagaatccca cttgccacat ccatgctaca tattcagtaa
     1501 tccccaaacg ccggccaggt ttaacaagca aaaactcttg cctacccgtt tcatccgaca
     1561 ttatgtacag actcaacaaa attgttcaac ccatgttaca catagagttg aaaatttagc
     1621 gcatttttgt cgttgatttt taaactataa ctaaggggca aacctgctaa acactttagg
     1681 tttcagctgt aagctggtgg tgaacaggct gccatgcgga tacaaaataa gcacccacag
     1741 gatgaacaaa aaaaaggtta ttatagctgg tggggggttt gccggaattc agcttgcccg
     1801 aaagctcgac cagcggctat tcgacgtgtt gttggtagat aaaatcaacc accaccagtt
     1861 tcagccgctt ttctaccagg ttgccacctc gcaacttgaa ccatcaagta tttccttccc
     1921 attaaggaac atctttagca ggaaaaaaaa tgttcagatt agactagctg aaatcctctc
     1981 cattgatcga aatgagagca aaataattac cagtataggc gaatttagct acgattacct
     2041 ggtgctggcc atgggctgca ccaacaactt tttcggaaat gaaaacatta aaaaaaatgc
     2101 tttcactcta aaaacaacct acgatgccat taccatcaga aaccacatca ttctaacatt
     2161 cgaaaaaatt gtatcggcta aggaggacga gctcgaagcg cttaacaacc ttgcaatagt
     2221 tggtgccggc ccaacaggag ttgaacttgc cggcgcattt gccgaaataa aaaagaatat
     2281 cctgccaaag gattacgcag gtgttgactt ttcaaaattc aacataattc taatcgaggg
     2341 tacccgaaac acgcttggca gcatgagcga tgcctcaaag gtggagtcaa gaaggtacct
     2401 acagcaaatg ggcgttacaa tactgacaga aactttcgta gccgactacg acggatatct
     2461 attaaagctg agcaacggag aaaccataaa aacaaaaacg gttatctggg ttgcaggagt
     2521 taccggaaac aacatcgaag ggctcccgga agctgcaagg gctcccggaa acaggataag
     2581 cgttgacagg tttaaccggg ttaacggatg cgataatgta ttcgcagtag gcgacatagc
     2641 ctacatggta acccccaagt accccaaagg gcacccacag g
//
LOCUS       DAOOQX010000233         2672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_46298_length_2672_cov_2.672526, whole genome shotgun sequence.
ACCESSION   DAOOQX010000233 DAOOQX010000000
VERSION     DAOOQX010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46298_length_2672_cov_2.672526"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..1825)
                     /locus_tag="PLX53_04675"
     CDS             complement(230..1825)
                     /locus_tag="PLX53_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOY72407.1"
                     /translation="MTKKLLYLCAFALIMPFGALSQNSSSFGAYKFVRFIQYLSGDYV
                     DTINVNKLVDNAIVEILSQLDPHSIYISKEDVQAMNEPLEGNFEGIGVEFNILNDTLM
                     VVNPISGGPSERVGILAGDRIIAIDGKSITNINLKITDVHKLLRGPKGTKVTVLIHRK
                     SLSTPVEFTITRDRIPIYSIDASYMAEQGIGYIKINRFAATTEQEFVEAMNKLNQQKM
                     NDLIIDLRNNGGGLLNSAYDLANYFFDPGKLIVYTEGRNSPRTNYFSTSKNKVFRGRV
                     VVLIDEGSASASEILAGAFQDWDRGIIVGRRSFGKGLVQNQLPLPDGSMIRLTVARYF
                     TPTGRAIQRQYKFGDLKDYYSDMTQRYDRGELFNKDSIHFADSLTYKTLIRHKTVYGG
                     GGIMPDVFIPLDTNYYSDYYGKLVRSGVINQFSLTWVDTHRNELLKEYPKFDKFKKNF
                     QVTEKMISELVALAESSGIPSDEKGLAQSANELKIQLKGLVAQSIFSTSHYFEVTNET
                     NETYQKALEIFHSWNDYKHLVQE"
     gene            complement(1890..>2672)
                     /gene="guaA"
                     /locus_tag="PLX53_04680"
     CDS             complement(1890..>2672)
                     /gene="guaA"
                     /locus_tag="PLX53_04680"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOY72408.1"
                     /translation="LLRKNEYQQVLDSFGSMGLNIVGVDASQQFYNELKGVTDPESKR
                     KIIGRNFIEVFEREAQKIQGVSWLAQGTIYPDVIESVSVNGPSAKIKSHHNVGGLPNQ
                     MNLKVVEPLRLLFKDEVRRVGKELGLSAAILNRHPFPGPGLGIRVIGEVTPQRVKLLQ
                     EADNIYIKALRESELYDKIWQAGAILLPVQSVGVMGDERTYENVVALRAVTSTDGMTA
                     DWYPFPHNFLSRVSNEIINKVRGINRVVYDISSKPPATIEWE"
BASE COUNT          692 a          627 c          597 g          756 t
ORIGIN      
        1 tcctgtttga gcctgggcta taacatcggt tgtctccttt aaaagtaccg gtatggtaat
       61 tttttgaatt ggcgaaggct gctcgaaacc cttagctttt attgctgcca atgccttttc
      121 ggataacccg agctctttaa attcatcaag tgcttccatt aatgtaatga taagttggac
      181 tcattcatag agtccagtta aattattagt aaaattaatt aaatttatgt tattcctgta
      241 ctaaatgctt gtagtcgttc cagctatgaa atatttccaa tgctttttga taagtttcat
      301 tggtttcatt ggttacctca aagtagtggc tggtagagaa aattgactgg gcaaccagcc
      361 ccttgagctg tatcttaagt tcattggccg actgggctaa acctttctca tcgctgggaa
      421 ttccgctact ctcggctaaa gccaccagct cggagatcat cttttcagtt acctgaaaat
      481 tctttttaaa cttgtcgaac ttggggtatt cttttaaaag ttcattcctg tgggtatcta
      541 cccaggttaa ggaaaattgg ttgataactc ccgaacgaac cagcttaccg tagtagtcgg
      601 aatagtagtt ggtgtcgagc ggaataaaca cgtcgggcat gatgcctccg ccaccataaa
      661 ccgttttatg cctgatgagg gttttatagg taagcgaatc ggcaaaatga atgctatcct
      721 tgttgaagag ttcgcccctg tcgtaacgct gagtcatatc ggaatagtag tccttcagat
      781 caccaaattt atactggcgt tgaatggctc taccggttgg ggtgaagtaa cgtgcaacgg
      841 tgagtcgaat catggaacca tcgggcaagg gcagctgatt ttgcaccaac cccttaccaa
      901 acgatcgcct tccaacaata attcccctat cccagtcctg aaatgctccg gcaagaatct
      961 cgcttgccga ggccgatccc tcgtcaatga gcactaccac ccttcccctg aataccttat
     1021 ttttagaagt ggaaaaatag ttggttcttg ggctattacg accctcggtg taaacaatca
     1081 gcttgccggg gtcgaaaaag tagttggcca ggtcgtaagc agagttaagc aatcctccac
     1141 cgttgtttct caggtctatg atcaggtcgt tcattttttg ctggttcaac ttgttcatag
     1201 cctcaacaaa ctcctgctcg gtggtagcgg caaatcggtt aatcttgatg tagccgatac
     1261 cctgctcggc catgtaggag gcatcgatgc tatagattgg aatacggtcg cgggtgatgg
     1321 tgaactccac aggtgtacta aggctcttac gatgaatgag cacggttacc ttggttccct
     1381 ttgggcctcg cagcaactta tgcacatcgg tgattttcag gttgatattg gttatagact
     1441 tgccatcaat ggcaattatt ctgtcgccgg ctagtatgcc aaccctctca gatggtccgc
     1501 ccgaaatggg attaaccacc atcagggtat cgttcagaat attgaactcc acaccaatcc
     1561 cctcgaaatt tccctcaagg ggctcgttca tagcctgaac atcctctttt gaaatataga
     1621 tggagtgcgg atcgagctgt gagagtattt caacaatggc attgtcaacc aacttgttaa
     1681 cgttgatagt atcaacgtaa tcgcccgata ggtactggat aaaccgaaca aacttgtagg
     1741 ctccaaagct tgaagagttc tgcgatagtg ccccaaaagg cattatcagg gcaaaagcac
     1801 atagatagag tagtttttta gtcatggtaa aggaaaattt ctacatgtag taaaacccga
     1861 aacaaatggt aaaagttcat agagtaagcc tactcccatt caattgtggc aggaggcttg
     1921 gagctgatat cgtaaaccac cctgtttatc cctctcacct tgttaatgat ttcgttagat
     1981 accctggaca gaaaattatg gggaaatggg taccaatcgg cggtcattcc gtcggttgaa
     2041 gtaacagctc taagggcaac cacattctcg taggtacgtt cgtcgcccat aactccaacc
     2101 gactgcaccg gaagtagtat ggcacctgcc tgccatatct tatcgtatag ttccgactcc
     2161 ctcagggcct tgatgtatat gttgtccgcc tcctgaagca gcttaactct ttggggggta
     2221 acctcgccta tcaccctaat gcccagtcct gggcccggga agggatgcct gttaagtatt
     2281 gcagccgaaa gccccaactc cttaccaacc ctacgaactt cgtccttgaa aagaagtcta
     2341 aggggctcaa caaccttaag gttcatctgg tttggcaacc cgcccacgtt gtggtgcgat
     2401 tttattttgg ccgaagggcc attaaccgag accgactcta ttacatcggg gtagatagtt
     2461 ccctgagcga gccagctgac cccttgaatt ttttgagctt cacgctcaaa gacctcaata
     2521 aagttacggc caataatctt acgtttggat tcgggatcgg taacaccttt tagctcgttg
     2581 tagaactgct ggctggcatc aactccaact atgttaagcc ccatgctgcc aaaagagtcg
     2641 agcacctgct ggtactcatt tttacgaaga ag
//
LOCUS       DAOOQX010000234         2655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_46708_length_2655_cov_4.521154, whole genome shotgun sequence.
ACCESSION   DAOOQX010000234 DAOOQX010000000
VERSION     DAOOQX010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46708_length_2655_cov_4.521154"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLX53_04685"
     CDS             <1..211
                     /locus_tag="PLX53_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72409.1"
                     /translation="TATRHNSPRLRRGSNSAARAFPRYVPPPTHLRVRLLCHFSNTCN
                     VQRFPPFRAPLRISFPGRAEEKGKG"
     gene            554..1021
                     /locus_tag="PLX53_04690"
     CDS             554..1021
                     /locus_tag="PLX53_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOY72410.1"
                     /translation="MSSFIGDYECKVDEKGRILFPASLRKQVKSALPDQFVVKKDIYE
                     DCLTLYTMEEWQRQNEIIRTNTNPYNREHNAFLREFFRGTAEITLDSSGRLLLPKRLL
                     DMIGVVRDVVLAGMDGKVEVWAKEKYEGIRLESVQFASLAEKILGSTTIRDEK"
     gene            1018..1917
                     /gene="rsmH"
                     /locus_tag="PLX53_04695"
     CDS             1018..1917
                     /gene="rsmH"
                     /locus_tag="PLX53_04695"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOY72411.1"
                     /translation="MTYHVPVLLAESIEGLNIRPDGTYVDLTYGGGGHSRHILERLGK
                     KGKLYAFDQDEDALTNASDDKRFTLIRGNFKYFRNFLRYYNVAKVDGILADLGVSSFH
                     LDTPERGFSFRFEGPLDMRMNKRASLTAKKVVNSYSIEELSLIFNQLGELPDGRRYAQ
                     AIYDGRKVKQLETIEELTQILKPLVPAKLQNKVLAKIFQALRIEVNHELDALKLMLVG
                     SAKCLGREGRLVVISYHSLEDRLVKNFLKFGNFEGEDRPDLMGNRVVPFEQVNRKVIV
                     PSFDEISANNRARSAKLRIGRRL"
     gene            1914..2285
                     /locus_tag="PLX53_04700"
     CDS             1914..2285
                     /locus_tag="PLX53_04700"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOY72412.1"
                     /translation="MSVSGETEEFVDIQPEKPREKKGFRFKDFLSGRILTHENVSSQL
                     PYILFLVLLAVIYIGNSYRNNQLLRNEQKIKIEVKNLRAESITTAAKYMSVSRQSEVV
                     KLVESKGLGLEESTIPPKKID"
     gene            2305..>2655
                     /locus_tag="PLX53_04705"
     CDS             2305..>2655
                     /locus_tag="PLX53_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72413.1"
                     /translation="MSIKKDIVTRITVVYILFALLGVGIFVKIIYIQFVEGSKLREKA
                     KAITYRDIIVEPNRGDILASDGRVLATSVPYYDIRMDLAASGLSDQVFFSNIDSLAIC
                     LSRFFGDRSKYAYRS"
BASE COUNT          736 a          566 c          667 g          686 t
ORIGIN      
        1 aactgccacg cggcacaact cgccccgcct tcggcgtggc tcaaacagtg ccgctcgggc
       61 attcccacgc tacgttccgc ccccgaccca tttgcgggtc aggcttttat gccatttttc
      121 taatacctgc aacgttcaac gattcccgcc tttccgagca ccgctgagaa tatctttccc
      181 cggtagggct gaagaaaagg gtaaagggta atacaaggat aaaggataaa aaaaattcaa
      241 gcaggtttca agaacttgtc cgcctgctgg cggattcacc cttcgacagg ctcagggttc
      301 agacttgtcc acctactggc ggattccaga acttgtccgc ctactggcgg aacttgtccg
      361 gctcctgacg gattccgtaa actgtccatc agctaatggg tttatcctta aaggttgacg
      421 gctaacatct caccgattgg cctaggtacg tcgctttcaa aaaaaagtta tcaacaaaat
      481 gcaaaaaagt gggtaaaagt ggataaaagt ggtaaagtat tgtatatttt taccatttaa
      541 atgccagctg gaaatgtcct cattcatcgg agactacgag tgtaaggttg acgaaaaggg
      601 tagaatactc tttcctgcat cgctacgcaa gcaggtcaaa tctgcgctgc ccgaccagtt
      661 tgttgtgaag aaggatattt acgaagattg ccttacgctc tacaccatgg aggagtggca
      721 gcggcaaaac gaaataataa ggacgaacac caacccttac aaccgggagc acaacgcttt
      781 tcttcgggag tttttcaggg gaacagctga aatcacgctc gattcaagtg gtaggctatt
      841 gctaccaaag cgactgctcg atatgattgg agtggtacgc gacgtcgtgc tggcgggtat
      901 ggacggaaag gtggaggttt gggcaaagga aaagtacgaa ggcatacggc tcgagtctgt
      961 tcagtttgcc agtctggccg aaaaaatttt aggttcaaca accataaggg atgagaaatg
     1021 acctaccatg taccggttct gcttgccgaa agcatagagg gtcttaacat cagacccgat
     1081 ggcacgtacg ttgacctcac ttatggcggg ggtggccatt cccgccacat acttgagcga
     1141 cttgggaaaa aaggaaagct ttacgctttc gatcaggatg aggatgccct tacaaacgct
     1201 tcagatgata aacggtttac cctaatcagg ggaaacttca agtactttag gaactttcta
     1261 cggtactata atgtcgctaa agttgacggt attctggccg atttgggagt ttcatcgttt
     1321 cacctggata ctcccgaacg cggattttcg ttccgattcg agggaccatt ggatatgcgc
     1381 atgaacaagc gggcctcact tacggcaaaa aaagtggtga acagctatag catcgaggag
     1441 ttaagcctga tttttaatca gcttggtgag ttacctgatg gtaggcgtta tgcccaggct
     1501 atatacgatg gacgtaaggt aaaacagctg gaaaccatcg aagagcttac acaaattttg
     1561 aaaccgcttg ttccagctaa acttcaaaat aaggttttgg ccaaaatctt tcaggctttg
     1621 cggattgagg taaaccatga gctggatgcg cttaaattaa tgttggttgg ctctgctaaa
     1681 tgtctcggga gagaagggcg gttggtggtt atttcgtacc actcgctgga ggaccgcctg
     1741 gtaaaaaact ttctcaagtt tggcaatttt gagggagagg atagacccga tttaatgggt
     1801 aacagggttg tcccctttga gcaggtgaac cggaaggtaa ttgttccctc gttcgatgag
     1861 atttctgcta acaatagagc gcgtagcgcc aagttaagaa taggaagaag gctatgagtg
     1921 taagtggcga aactgaagaa tttgttgaca ttcaacctga aaaacccagg gaaaagaagg
     1981 gttttcggtt taaggacttt ttaagtgggc ggattctcac ccatgagaat gtttcgtcac
     2041 agctgcccta catacttttt ttggttctcc ttgccgtaat ctacataggc aatagctacc
     2101 ggaataacca gctgttgcga aacgagcaaa aaattaaaat tgaagtaaaa aacctcaggg
     2161 cagaatccat tacaactgcg gccaagtaca tgtcagtaag ccgtcagtcg gaggtggtaa
     2221 aacttgttga aagtaagggg ctggggcttg aggagtccac catcccccct aaaaaaattg
     2281 attagccaaa agcagcctgc aggtatgtcg attaagaagg acatagtaac ccgaattaca
     2341 gtagtttaca ttctattcgc actgctgggc gttggtattt ttgttaaaat catctacata
     2401 caatttgttg aggggtcgaa gcttagggaa aaggccaaag ccataaccta cagggatatt
     2461 attgtggaac ctaaccgggg tgatattctg gcatcggacg ggagagtgct ggccacttct
     2521 gttccctact acgatattcg gatggatttg gcagccagtg ggttgtctga tcaggtattt
     2581 ttctcaaata ttgattctct ggctatttgc ctatcccgat tttttggtga tcgttcaaaa
     2641 tacgcatatc gttcg
//
LOCUS       DAOOQX010000235         2655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_46717_length_2655_cov_3.080769, whole genome shotgun sequence.
ACCESSION   DAOOQX010000235 DAOOQX010000000
VERSION     DAOOQX010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46717_length_2655_cov_3.080769"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLX53_04710"
     CDS             <1..371
                     /locus_tag="PLX53_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOY72414.1"
                     /translation="TSAECNPVASVDYLRQVKEVFGAGVVRYTALSRQVIKRVAFCGG
                     SGASLIGKAIGSGADIFITSDIKYHQFFDADGRIIIADIGHFESEQFTIELFYEYLLK
                     KFPTFAVLKSRVNTNPINYL"
     gene            391..1149
                     /locus_tag="PLX53_04715"
     CDS             391..1149
                     /locus_tag="PLX53_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOY72415.1"
                     /translation="MKNKPAEPTAELSVEEKLRFLYMLQQVDSKIDRIRMLRGELPLE
                     VQDLEDEIEGLETRIDNLKQEIKSLESEVSAKKQEIKDANTLIKKYQEQQKTVRNNRE
                     FDSLSKEIEYQTLEIELCEKRIKEFTQQVKDKTLVIEQSQKLLEERKLDLKGKRMELD
                     NIINETQKEEEELIRKSEEYGKIIEPRLYTAYRRIRGNARNGLAVVTVQRDACGGCFN
                     KIPPQRQLDIKIHKKIIVCEYCGRILVDDSLASM"
     gene            complement(1206..1901)
                     /gene="rsmI"
                     /locus_tag="PLX53_04720"
     CDS             complement(1206..1901)
                     /gene="rsmI"
                     /locus_tag="PLX53_04720"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOY72416.1"
                     /translation="MSKLFIVPTPIGNLGDITLRALEVLRSVSLVLAEDTRTSGNLLK
                     HYSIETPLRSHHKFNEHQSVEAIAEQIAAGLDVALISDAGTPGISDPGFLLVRTCLER
                     GVEVECLPGATAFVPALVQSGMPCDRFVFEGFLPQKKGRSKRIEALQDEDRTMVFYES
                     PFRLVKLLEQLMAVFGPDRRVSVSRELTKLHEETMRGTLAEVAAHFNSKTVKGEVVVV
                     VQGLGRNKSLDEE"
     gene            complement(2047..>2655)
                     /locus_tag="PLX53_04725"
     CDS             complement(2047..>2655)
                     /locus_tag="PLX53_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72417.1"
                     /translation="LLLFSVEKQAEPTHQAHSQHTVKANAETTTQGIKRDQPLSTPSH
                     NVEPVAPAGSYSKKPPEQIQKFREKAPSTNESKSIEVEPTPGAASTDRPATLADKFQD
                     KQKSLNETIANQARRSDMGTKLQNKPITDLAKAIGINDKFLFTNELFDGDSALYAETV
                     NRLNEMDDLNEAIIYLQDNFSWSATSEAASKFIDLLRRKFAL"
BASE COUNT          701 a          582 c          657 g          715 t
ORIGIN      
        1 gaacctccgc agaatgcaac cctgttgctt cagttgacta tttaagacag gtaaaggaag
       61 tgtttggcgc tggtgtggtt cgctacaccg cgctttcacg tcaagttatc aaaagggttg
      121 cattctgcgg aggttcaggt gccagcctga ttggaaaggc tattgggtcg ggcgccgaca
      181 tatttattac ctcagatatc aagtaccacc aattcttcga tgccgatggc agaattatca
      241 ttgccgacat tgggcacttc gagagcgaac agtttactat tgaattattt tatgagtacc
      301 ttttgaaaaa attccctacc tttgcggtct taaaatcaag ggtaaatacc aatccaatta
      361 actacttata gcttgtaaaa tatggcagca gtgaagaaca aaccagctga acctaccgcc
      421 gagctttcgg tggaggagaa attacgtttt ctgtacatgc ttcagcaggt tgattctaag
      481 attgaccgga ttagaatgct gaggggagaa cttccccttg aggttcagga cctggaggat
      541 gaaattgaag ggctcgaaac cagaattgat aacctgaaac aggaaatcaa gagccttgag
      601 agcgaggtgt ctgccaagaa acaggaaatt aaggatgcca atacgcttat aaaaaaatat
      661 caggagcagc aaaaaactgt taggaacaac cgggagtttg attcgctctc aaaggaaatt
      721 gaataccaaa cattggaaat tgaactttgc gaaaagcgaa ttaaggagtt tacccaacag
      781 gtaaaggata agactcttgt tattgagcag tcgcaaaagt tgcttgagga gcgaaaactc
      841 gatctaaagg gaaaaagaat ggagctcgat aatattataa acgagaccca gaaggaggaa
      901 gaggagctta ttaggaaatc cgaggagtat ggaaagatta ttgaacccag gctttacaca
      961 gcataccgtc ggattagggg aaatgcacgc aacggcttgg ctgttgttac cgtgcagcgt
     1021 gatgcctgcg ggggttgctt caacaagatt cctccccagc gccagctcga cataaagatt
     1081 cataagaaaa ttattgtgtg tgagtactgt ggccgaattc tggtggacga ctctttggcc
     1141 agcatgtaat agaaacaacc ttcgtatgcc tccaagcggt ttagtcttgg gggctttttt
     1201 attgcttact cctcgtcaag gcttttattc cttcctaaac cctgaacaac cactaccact
     1261 tcacccttaa cggttttcga gttgaagtgt gctgccactt cggctagtgt tccccgcatt
     1321 gtttcctcat gcagcttggt cagctccctc gaaacgctaa ccctccggtc ggggccgaaa
     1381 acagccatga gctgctccag cagctttacc agccgaaagg gcgattcgta aaacaccatc
     1441 gtgcgatcct cgtcttgaag tgcctcaatt cgcttgctgc gacccttttt ctgtggcagg
     1501 aacccttcaa acacaaatct atcgcagggc ataccgctct gaaccagcgc aggaacaaaa
     1561 gctgttgccc cgggtaggca ttccacctca actccccgtt ccaggcaagt gcgcacgagg
     1621 aggaaacccg gatcggaaat gccaggtgtt ccggcatccg atatcagggc aacgtcaagt
     1681 ccggcggcta tctgttcggc aattgcttca accgattggt gctcgttgaa cttatgatgc
     1741 gatcttagtg gggtttcgat gctgtaatgc ttaagcagat tcccactggt gcgggtatcc
     1801 tctgcaagta ctagcgaaac cgaacgtagt acctccagcg ctcttaacgt tatatcacct
     1861 aaatttccta ttggcgtagg aacaatgaat agtttggaca tgggttggag gttagcgtgt
     1921 ttttttactt tggtgaagtt tcaatttcaa acattttggg tgggtggggc tttacatctg
     1981 ccagcgctat caaatgttaa aaaatgaaca ttactaaaaa aagtaacagg gaagggcagg
     2041 aggtagttac agtgcaaact tcctgcgcaa taaatcgata aattttgatg cagcttcgct
     2101 ggttgcactc cagctaaagt tatcctgaag gtaaataatg gcctcgttca agtcgtccat
     2161 ctcgttaaga cggtttaccg tttcggcata gagcgccgaa tcgccatcaa acagctcgtt
     2221 ggtgaacagg aacttatcgt taatgcctat tgccttggca aggtcggtaa tgggcttgtt
     2281 ctgcagcttg gtgcccatat cggacctgcg cgcttggttg gcaatagttt cgttaagtga
     2341 cttttgcttg tcctgaaact tatcggccaa cgttgccggt cgatcggtgc tggctgctcc
     2401 gggggttggc tcaacctcaa tacttttact ttcatttgta gatggagcct tttccctgaa
     2461 cttttgtatt tgctcgggtg gtttctttga gtatgaacca gctggagcaa caggttcaac
     2521 attatggctt ggggtggata aaggctgatc tcttttgatg ccttgagttg tagtttcggc
     2581 atttgcctta acggtatgct ggctatgggc ttgatgggta ggttcagcct gcttttcaac
     2641 gctaaacaac agcag
//
LOCUS       DAOOQX010000236         2648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_46900_length_2648_cov_2.586579, whole genome shotgun sequence.
ACCESSION   DAOOQX010000236 DAOOQX010000000
VERSION     DAOOQX010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46900_length_2648_cov_2.586579"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="PLX53_04730"
     CDS             <1..932
                     /locus_tag="PLX53_04730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOY72418.1"
                     /translation="TGYPQFSSPDALVDGLINAGVNVLVTANNHSADRGAKGIQRTVE
                     TLDRKGIIHTGTFSDSSDRLKNNPLILEKNGMRIALLNYTYGTNGLPVPAPCIVNKID
                     TLVIIRDIAMTRLMNADEIVVFIHWGNEYERYPSAAQVAMANFLNRQGVRIVIGSHPH
                     VVQPMEATLDTDSTTGRIVVYSLGNFVSNQRRRYCDGGALAAVKLVKKSTGKSEITAA
                     GFLPVWVHTPSTASHKTFQVLPVSIYETAPKGYFSADELAQLTQFATDTRALMHEKNL
                     NFPELVFYNGSWIIPDNRDKLPSTRLTTGQSHF"
     gene            1157..>2648
                     /locus_tag="PLX53_04735"
     CDS             1157..>2648
                     /locus_tag="PLX53_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOY72419.1"
                     /translation="MKQRFSFLIRFFLFWLIYFIVFRAVFLVYQLPLTRGIGMGDALM
                     IFTRGIWMDTSLAGYILLLASLFMALLYFVHGRFIRTVYTTYTIILLVLFSVITISDL
                     ELYRNWGFRIDATPLLYLKTPKDAMASLKGWVILVLAIAAAIAVISSMFFYRKWVAKP
                     LNHIEKGKWWYFPIFTFIAACMIIPIRGGFGIAPMNPGKVYFSQNVYCNHAALNPVWN
                     MMYSVSKSGKMSKQYPTEIKPSEAKQLFTSMMESDSTNYVLSSNRPNIVVILLESFSA
                     KLIEPLGGKSGVTPCFNRLSEQGILFTDAFASGDRSDKGIIAVLSGFPAQPTQSIIKY
                     PTKSRKLPTISGVLAENGYNNTFYYGGDPDFANIRSFLYHAKFNRIVTQDEFPKKYRN
                     SKWGVHDEYVFNYLLTDLDSARAPFFKFFFTLSSHEPFEIPAKPKFKGSDEVNQYLSS
                     AYYTDSCLGNFFNIAQKKNWYKNTIFILIADHGHRHLGNHPNYAVEK"
BASE COUNT          764 a          615 c          533 g          736 t
ORIGIN      
        1 atacagggta tccgcaattc agctcacccg atgctcttgt agatggtcta ataaatgctg
       61 gggtaaacgt actggttact gctaataacc attccgcaga caggggcgct aagggaatac
      121 aacgaacagt tgaaaccctc gaccgcaaag ggataattca caccggaacc ttttccgatt
      181 catccgacag gttgaagaat aaccctttaa ttctcgagaa aaatgggatg cgtattgccc
      241 tgctgaacta cacctatgga accaacggac tgcccgttcc ggcaccctgt attgtcaata
      301 agatagacac tttggtaata ataagggata ttgctatgac aaggttgatg aatgccgatg
      361 agatagtagt gttcatccac tggggaaacg aatatgagcg gtatccatcg gctgcgcaag
      421 tagcaatggc taattttcta aaccgccagg gggtaagaat agtaattggc tctcatcccc
      481 atgtagttca acccatggaa gccaccttag ataccgatag cacaaccggc agaatagttg
      541 tttactccct aggaaacttt gtgtcgaacc aacgaagacg ctactgtgat gggggagcgc
      601 ttgcagccgt aaagctggtt aagaaatcta caggtaaatc agaaattact gctgcaggtt
      661 ttcttcccgt atgggttcat acgccctcaa ccgcctccca caaaacgttt caggttcttc
      721 cagtttcgat ctacgaaaca gctccaaaag gctacttttc tgccgatgag ctggcacagc
      781 tcacccaatt tgccaccgat actcgtgctc tgatgcacga aaaaaatctg aacttccccg
      841 agcttgtctt ttacaacggc agctggatta taccagacaa tagagacaaa ttacccagca
      901 ctaggttgac caccgggcaa tctcattttt agcataaatc aaagcgaata atattttcct
      961 gacgaagcat tcccttcgct ggtaatatta tatgctacca acctgcatgt atttataaaa
     1021 aaaacctgcc acacgagcta ccggaaatga atgcttaaaa atttaaaatt ttatacctgc
     1081 gtttatacct acccctttta tagctatgtt tgcaggattt ttaattaatt attaacatca
     1141 acttaacgct aagttaatga aacaacgttt ctcattcctg ataaggtttt tccttttctg
     1201 gctgatttac ttcatagtct ttagagcagt atttttggtt taccagcttc cgttaacgcg
     1261 gggtataggc atgggtgatg cactaatgat tttcacgcgt ggaatatgga tggacacttc
     1321 gctggcagga tacatactgc tgctggcctc gctgttcatg gcattactat attttgttca
     1381 tggacgtttt attcgaacgg tttacacaac ctataccatt attttgctgg ttctattttc
     1441 ggttataacc atttcggacc tggagcttta ccgtaactgg ggtttcagaa ttgatgccac
     1501 tcccctactc tacctcaaaa ctcctaaaga tgccatggcc tctttaaagg gttgggtaat
     1561 tttagtgcta gccattgcag cggcaattgc ggttatctca tctatgtttt tttaccggaa
     1621 atgggttgcc aaacccctca accacattga aaaggggaaa tggtggtatt tcccgatttt
     1681 cacattcata gccgcctgta tgattattcc tattcgcggg ggattcggaa ttgcaccaat
     1741 gaatcctggc aaggtttact ttagccagaa cgtttactgc aaccatgcag cattaaaccc
     1801 agtatggaac atgatgtact ctgtttccaa atcgggaaaa atgagcaagc aatacccgac
     1861 ggagattaag ccaagcgaag caaaacaact tttcaccagc atgatggaat ccgactccac
     1921 caactacgtt ctctcctcta acaggcctaa tattgtggtt atcctgctcg aaagctttag
     1981 tgctaaatta attgagcccc tcggaggaaa aagtggcgta actccatgtt ttaacaggct
     2041 ttcagaacaa ggtatattgt ttacagatgc ctttgcaagc ggtgatagga gcgacaaagg
     2101 aattattgcg gttctttcag gctttccggc gcaacccaca caatcaatta ttaagtatcc
     2161 aaccaagtcg cgtaagctac ccactataag cggtgtgctg gccgaaaacg gatacaacaa
     2221 cactttctac tatggaggcg accccgattt tgccaatatc cgttcgttcc tataccatgc
     2281 aaagtttaac agaattgtaa cccaagacga attccctaaa aaataccgca actccaagtg
     2341 gggtgttcac gatgagtatg ttttcaacta cctgcttacc gatctcgata gcgccagggc
     2401 tcctttcttt aaattctttt ttacgctaag cagccatgag ccctttgaaa ttccggccaa
     2461 accaaaattt aaaggtagtg atgaggttaa ccagtaccta agctcggcat actataccga
     2521 tagctgcctg ggaaattttt tcaatattgc ccagaaaaag aattggtaca aaaacaccat
     2581 tttcatactc atagcagatc atggccatag gcacctagga aatcatccca actatgccgt
     2641 tgaaaaat
//
LOCUS       DAOOQX010000237         2647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_46907_length_2647_cov_3.915123, whole genome shotgun sequence.
ACCESSION   DAOOQX010000237 DAOOQX010000000
VERSION     DAOOQX010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46907_length_2647_cov_3.915123"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="PLX53_04740"
     CDS             <1..1520
                     /locus_tag="PLX53_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOY72420.1"
                     /translation="ETMVTDYPLVDITQRIARVEPVKYPMAGMKSHEVTLRVYNLSTH
                     SNIEIKTGLPAEQYLTCVTWSPDEKSIFIATLNREQNHMRLNRYSAQTGELMATLFEE
                     TDSEYVEPQQPLAFLDDNSGRFIWQSRRDGWNHLYLYDVNGKLLRQLTRGEWEVTNIN
                     AIDLPNGKIYITATRESPIERQVYAVDLKKDNITMLTQGAGFHNALFSPDCKYFVDFY
                     SSISIPNRVNLCTGKGKIVKNLVNAPNPLASYNVSLPELLTLKADDGTTLYGRMIRPV
                     NFDSTLRYPVVVYVYGGPHSQLVRNSWMGGASLWECYMASRGYILFTLDNRGTSYRGS
                     NFEQTIHRRLGTIEIADQLAGVNFLKSLPYVDSTRIGVHGWSFGGFMTISLMLRAPEF
                     FKVGVAGGPVTDWKFYEVMYGERYMDTPQENPDGYSTSDLKQYVKNLKGKLLIIHDDM
                     DKTVVPQNSLTLLHEFVKSNVQVDFFLYPQHEHNVMGKDRVHLIDKIIRYFDDNL"
     gene            complement(1849..2421)
                     /locus_tag="PLX53_04745"
     CDS             complement(1849..2421)
                     /locus_tag="PLX53_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72421.1"
                     /translation="MFWPKIDSIENELINKQNAEVIISSYEGEPDETCNYYLDTVNFT
                     LRSEYNSSGVSISELRILFSDQTVVVIRSSFSGTSSFTNQNDLLIYNYNFNEKTFSLD
                     TINIKLFNVELKDYFKENTPDSIISEFEGHSSYFFHIIYNETGIAENQLFDNSFEKNI
                     MNNNWLKGNKITFYYKDNKFIKTEPYFSNE"
BASE COUNT          762 a          567 c          546 g          772 t
ORIGIN      
        1 acgagacaat ggtaaccgac taccccttgg tggacataac gcagcgcatt gcaagggtag
       61 agcctgtgaa gtaccccatg gccggcatga agagccacga ggttaccctc agggtttaca
      121 accttagcac ccattcgaac atcgaaatta aaaccgggct acccgcagaa cagtatctta
      181 cctgcgttac gtggagtccg gatgaaaagt cgatttttat tgcaacgctg aacagggaac
      241 agaaccacat gcggctcaac cggtatagcg ctcaaaccgg agagctgatg gcaacccttt
      301 ttgaagaaac cgattctgaa tatgttgaac cccaacaacc gctggcgttt cttgacgata
      361 attcgggtag gttcatatgg caaagccggc gcgatggctg gaaccacctc tacctttacg
      421 atgtaaacgg aaagttgctc aggcagctaa cccgtggcga gtgggaggtt accaatataa
      481 acgctattga tcttccaaac ggtaaaattt acattaccgc aactcgtgaa agcccaatag
      541 aaaggcaagt ttacgctgtt gacctaaaaa aggacaacat taccatgcta acccaggggg
      601 cagggtttca taacgcactt ttcagtcccg attgtaagta ttttgtcgat ttctactcct
      661 ccatttccat tccaaacagg gttaaccttt gcacgggcaa gggaaaaata gttaaaaacc
      721 tggtcaatgc ccccaaccct ctggccagct acaacgtatc ccttcccgaa ctcctcacgc
      781 ttaaggctga tgacggcaca accctgtacg gccgtatgat tagacctgta aattttgact
      841 ctacactaag gtaccccgtg gtggtttacg tttacggagg ccctcactcg caactggtac
      901 ggaactcgtg gatgggtggg gcaagccttt gggagtgcta catggcttca aggggctaca
      961 tccttttcac cctcgataac cggggaactt cctaccgggg aagcaatttt gagcaaacaa
     1021 tccaccgccg gctaggaacc atagaaattg ccgaccagct ggctggggta aacttcctga
     1081 aatccttgcc ctatgttgat tccacaagaa ttggtgttca tggatggagt tttggcgggt
     1141 ttatgaccat ttcgctcatg ctgcgtgcac ctgaattctt taaggttgga gttgctggtg
     1201 gcccggttac cgattggaag ttctacgagg tgatgtatgg tgagcgttat atggacaccc
     1261 cacaggaaaa ccccgacggg tatagcacgt ccgaccttaa acagtacgta aaaaacttaa
     1321 agggaaagct cctcatcatc cacgacgata tggacaaaac ggtggtgcct cagaacagcc
     1381 tcacgctgct tcacgagttt gttaaaagca acgttcaggt cgatttcttc ctctaccccc
     1441 agcacgagca taacgtgatg ggtaaggaca gggttcacct gattgataaa attatccgct
     1501 acttcgacga caacctgtaa aaccagctac cctggttttt gctacaagct gtgcagagca
     1561 aatgtgagat gttcccttaa ggtgaacggc tggcttcaca gaactttttt gctgctgcac
     1621 aattgccttc gccgagctcc ccgccgactg gcgggtcggt tcttgcagta tcggcgttcg
     1681 gcttaggcta aagcctaagt ttaatataac cttgcaggct gagcctgcat tcgataggct
     1741 gacggcggga cagcgtctaa atcccacgat acgcagtatc gaaaatgcga ttagcttgct
     1801 aatccgcatg aagccagcat tttttacacc ctttgttagg ggaagttttt attcatttga
     1861 aaaatatggc tctgtcttaa taaatttatt gtctttgtaa taaaacgtta ttttgtttcc
     1921 tttaagccaa ttattattca taatattttt ttcgaaagag ttatcaaaaa gttggttttc
     1981 tgcaatgccc gtttcgttat atataatatg aaaaaaatag cttgagtgtc cttcaaattc
     2041 tgaaattatt gaatcaggag tattctcttt aaaatagtct tttaattcca cattaaatag
     2101 tttaatatta atagtatcta gtgaaaatgt cttttcattg aagttatagt tataaattaa
     2161 taagtcattc tgatttgtaa aagaacttgt tccactaaaa cttgatctaa taactacaac
     2221 tgtttggtca gagaataata tgcgtaattc agaaatggat actccactag aattatattc
     2281 acttcttagt gtaaagttta ctgtgtctaa atagtaatta catgtctcat caggttcacc
     2341 ttcatatgaa gaaattataa cttctgcatt ttgtttgttg attagttcat tttctatcga
     2401 atcaattttg ggccaaaaca atttaaagat tgatattaaa gaatctgcat ttatcttatt
     2461 ctctgttaat ttaatcgaac ctttgtctta tgcaacaatt aaattattta ttaaagaaat
     2521 tattaaaaat aataggctaa aaataatcaa taatatattt ttattatttt tctttgtagt
     2581 taaaaatttc aaaattacct ctaacggatg gcagtatggt tagttgccga tttagaagca
     2641 ctttcct
//
LOCUS       DAOOQX010000238         2644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_46985_length_2644_cov_3.019312, whole genome shotgun sequence.
ACCESSION   DAOOQX010000238 DAOOQX010000000
VERSION     DAOOQX010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46985_length_2644_cov_3.019312"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1633
                     /locus_tag="PLX53_04750"
     CDS             <1..1633
                     /locus_tag="PLX53_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOY72422.1"
                     /translation="PMESSMAQVYTYVKKERENSQQQVILLDNGDILQGQPTVYYSNY
                     IDSSSCNIVSRVFSYMGYDAATVGNHDIETGPLVYRKVERELAIPWMAANAINTITGE
                     PAFKPYTIIKRSGLKVAILGLITPGIPSWLPRNLWPNMRFDDMVESATYWVQQIQEKE
                     KPDVLVGLFHSGYNSSYGGANAETPLNENASLLVARKVPGFDVIFIGHDHDKLITKVE
                     NVQGDSVLVLDPTSSARYISEATIQLSLDKKKRVVSKNITGTLVETRRFSPDSAFMAE
                     FKSCYQKVDSFVNQPLGEFTQTISSANAYFGPTAFMDFIHSAQLSITNADISFAAPLS
                     FVSTINKGIVRVSDMFKLYRFENFLYTMNLTGAEVDAYMEYSASLWFNTMKDANDHLL
                     RFRQNADGSMATDNRGKVELASRYYNFDSAAGIVYTVDVSKPDGNKVTILSMVNGEPF
                     DTAKTYRVAINSYRGNGGGGHLTKGCGLSAEDIQKRIVASTDKDLRFYIMKFIEQQKV
                     ITPKSFGTWKIIPEEWAASGAARDHKLLFSYTGND"
     gene            1635..2273
                     /locus_tag="PLX53_04755"
     CDS             1635..2273
                     /locus_tag="PLX53_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOY72423.1"
                     /translation="MGDHTTINPELESGKVLPLMEEFYTLQGEGFQTGRAAYFIRIGG
                     CDVGCSWCDSKQSWNSRLFPPVSVDEIVQRAIAHPARAVVVTGGEPTLYPLGYLCSEL
                     KKHNIQTFLETSGAYTLTGQWDWVCLSPKQQQPPVDLNYSKANELKVIIAKQADLCWA
                     EANAQRVRPDCMLFLQPEWSVHESITPLLVDYIKQNPKWRISLQAHKFMHIP"
     gene            2445..>2644
                     /locus_tag="PLX53_04760"
     CDS             2445..>2644
                     /locus_tag="PLX53_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72424.1"
                     /translation="MKKILLLLIFLLPMLLSAQSPKNLSTKNKRAVARYNEALAAYNR
                     YNLNEAERLLKESIKIEHNFIEA"
BASE COUNT          778 a          620 c          601 g          645 t
ORIGIN      
        1 gccaatggaa tcgagcatgg cacaggttta cacctacgtt aagaaggaaa gggaaaatag
       61 tcaacagcag gttatcctgc tggataatgg cgatattctt cagggacaac caaccgttta
      121 ctattccaac tacattgaca gctcaagttg taacattgtt tcgagggtat tcagctacat
      181 gggctacgat gcggcaaccg ttggcaacca cgatattgaa actggcccgc tggtttaccg
      241 aaaagtagaa agggaacttg ccattccgtg gatggcagca aatgcaataa acaccattac
      301 aggggaacct gcatttaaac cctacaccat cattaaacga agcggtttaa aagtggccat
      361 tttggggtta ataacccccg gcattcccag ctggcttccc cgaaatcttt ggcctaacat
      421 gcgtttcgac gatatggttg aatcggctac atactgggtt caacaaatac aggaaaagga
      481 gaaacccgat gtgctggttg gactcttcca ctcgggctat aactccagct atggaggtgc
      541 aaatgccgaa actccgttga atgagaatgc atccctgctg gtagccagaa aggtaccggg
      601 atttgatgtc atcttcattg ggcacgacca tgataagctg ataaccaaag ttgaaaacgt
      661 gcagggcgat tccgtgttgg ttctcgaccc aacctcatca gcacgctaca tctcggaggc
      721 aaccatacaa ctttcattag acaagaaaaa gagggtagtt tcaaaaaata ttaccggaac
      781 actggttgaa acccgaaggt tttctcccga ctccgcattc atggcagaat ttaaaagttg
      841 ctaccaaaag gttgactcct ttgttaacca gcccctggga gaatttacac aaaccatctc
      901 cagcgcaaat gcatactttg gaccaaccgc tttcatggat tttattcaca gcgcccagct
      961 cagcatcacg aatgccgata tttcgtttgc tgcgccgtta tcgtttgttt caaccataaa
     1021 caaagggatt gtgagggtta gcgacatgtt taaactttac cggtttgaaa attttctata
     1081 caccatgaat ctcaccggtg ccgaggttga tgcctacatg gagtactctg catcactatg
     1141 gttcaacacc atgaaggacg ccaacgacca cctgttacgg ttcaggcaaa atgccgatgg
     1201 tagcatggca acggataaca gggggaaagt tgagctagct agccgctact acaacttcga
     1261 ttcagcagca ggaattgttt acacagttga tgtatcgaag cccgatggca ataaggttac
     1321 catactttct atggtcaatg gagaaccgtt cgatacagcc aaaacctacc gggtggcgat
     1381 taactcatac cgtggaaatg gtggaggtgg acatttgact aagggctgcg gactctcggc
     1441 cgaggatatt cagaaacgta ttgttgcatc aaccgataag gacctgcgct tttacatcat
     1501 gaagttcatt gagcaacaaa aagttatcac cccaaaatca ttcggtactt ggaaaattat
     1561 tcctgaggag tgggctgcca gcggagctgc acgtgaccac aagttgctct tttcatacac
     1621 aggcaacgat tagcatgggt gatcatacaa ccataaatcc tgaactcgag tcgggtaaag
     1681 tattgcccct tatggaggag ttctacaccc ttcagggtga gggctttcaa acagggaggg
     1741 cggcttactt tattcgaatt ggtggatgcg acgtgggctg ttcgtggtgc gattcgaagc
     1801 aatcgtggaa ctccagacta ttccctccgg ttagcgtgga cgagatcgtt caaagggcaa
     1861 tagcacatcc tgccagggca gtggtggtaa cgggtggcga accaacactt taccctctcg
     1921 ggtacctttg cagcgagctt aaaaaacata acatccaaac attccttgaa acttcaggcg
     1981 cttacacgct tacaggtcaa tgggattggg tttgcctatc tcctaaacag cagcagccac
     2041 cagtggattt aaactattca aaggccaacg aactcaaggt gattattgcc aaacaggccg
     2101 acctgtgctg ggcagaagca aatgcccaaa gggttcgacc agactgcatg ctttttctcc
     2161 aacccgaatg gagcgtccac gaaagcatta cccccttgct ggtggactac ataaagcaaa
     2221 acccaaaatg gagaatatcg ctgcaggcgc ataagttcat gcacattccg taagctgaat
     2281 aaaattcgct ctttaactca ctactacaaa aaccaagcgg ttgttaatag caaggagtat
     2341 ccataaaaaa taacccaaat catacatttt acctactttc gtttactttt gtatattgta
     2401 gcgtagaatt tagctgctgg cacactactg gacatctcca aagcatgaaa aaaatactcc
     2461 tgctcctgat atttctgctg cccatgcttc ttagtgctca atcacctaaa aatctgagca
     2521 ctaaaaataa gcgggcggtg gcacgctaca acgaagcact tgctgcatat aaccgctaca
     2581 acctgaacga agcggaaagg ttgctgaagg agagtatcaa aatagagcat aattttattg
     2641 aagc
//
LOCUS       DAOOQX010000239         2640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_47089_length_2640_cov_2.471954, whole genome shotgun sequence.
ACCESSION   DAOOQX010000239 DAOOQX010000000
VERSION     DAOOQX010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47089_length_2640_cov_2.471954"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLX53_04765"
     CDS             complement(<1..358)
                     /locus_tag="PLX53_04765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY72425.1"
                     /translation="MLTIATIAITLLYVVLIVLLIVGLVQPSAGHNQAFGQLAQPVSV
                     VVAFRNEAPNLTRLLEALSRQTLDASKWELIMVDDGSTDGGDKIAQSFHNLPGLRLVK
                     LQKGITGKKQALLFGVE"
     gene            complement(595..1488)
                     /locus_tag="PLX53_04770"
     CDS             complement(595..1488)
                     /locus_tag="PLX53_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOY72426.1"
                     /translation="MVDKSYIHSLITLLDDPDPEIFNAVSEKIITQGITVVPLLEGAW
                     GSSDSRIFQERLENLIQTIQFNTVANALTQWKDSESNNLLEGAVLVARLQFPDLSLQP
                     IELEIKKLQKDIWLEINSNLTALEKVRVINHVIFTIHKYEPNTSNFFLPGNHLINHVV
                     ETKRGGPIMLAILYAEIAQRLGLPISCVNLPKNFVLAYQDRYRVPAERKGKDPESILF
                     YINPFNNGSVFSRNEIELFLSRQQLEPKPEYFLPCANTTTISQLVFNLIYSLERNGNR
                     SKANEYKKLLQILEKDGEEQG"
     gene            complement(1499..2374)
                     /locus_tag="PLX53_04775"
     CDS             complement(1499..2374)
                     /locus_tag="PLX53_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOY72427.1"
                     /translation="MRVIESSELILNQDGSVFHLHLKPEQLADTVILVGDPGRVETVS
                     SYFDRVELTVSNREFKTCTGTYKGKRISVVSTGIGTDNIDIVVNELDALANIDLASRT
                     VKANHTKFTLVRLGTSGALQPEIELGSSVLTEISIGFDGLLNFYANRNGICDLEMEQA
                     FLKHTQWNPLCAKPYFCHSSRKLVELFAGITVKGVTISAPGFYGPQGRVLRLPLADPT
                     LNDKIVDFRYKGYKITNFEMESSAINGLSLLLGHDSVTICTIIANRVVKDAKVDYQLA
                     MKNLIENTLNRLASI"
BASE COUNT          663 a          622 c          630 g          725 t
ORIGIN      
        1 attcaacccc aaataacaag gcctgctttt tcccggtaat gcccttctga agtttcacaa
       61 gccgtaaccc cggcaggtta tgaaatgatt gtgcaatttt atcaccccca tctgttgacc
      121 catcatcaac catgattagc tcccacttgc ttgcatcaag ggtttgacgg cttaacgcct
      181 caagcaatcg ggtaaggttt ggagcctcgt tccgaaacgc cacaaccacg ctaaccggtt
      241 gagccagctg gccaaatgct tggttgtggc cagctgacgg ttggactaac ccaacaatca
      301 gcagcacaat caggacaacg tagagcaggg tgatggcaat tgttgcgata gtaagcatga
      361 agcaaaatgc cttgtactgg gttaaaatta gtgcaaataa aatgagaaga agctaaatgc
      421 gtaaaaaata tgatgagcta aaattttgcc cggagtttgt ctaaggaact tgcccttagc
      481 agtagggcaa actcactata acacctgtcg aaaggagaat ccgccagtag gcggacaagt
      541 tccgtcagga gccggacaag ttccgccagc aggcggacaa gtttatctta tcctttaccc
      601 ctgttcttcc ccatcctttt caaggatctg aagcaatttt ttgtattcgt tggccttgct
      661 gcgattaccg tttctctcca gagagtaaat caggttgaat accagctgtg aaattgttgt
      721 tgtgttggca catggcagaa agtattcggg tttaggctcc agctgctgtc ggctaaggaa
      781 tagctctatc tcgttacggc tgaaaaccga accgttgttg aatgggttga tgtagaacag
      841 gatactttca ggatccttac cttttctttc agcgggcacg cggtacctgt cctgataggc
      901 aagcacaaag tttttaggaa ggtttacgca cgaaatgggc aaccctaaac gctgggcaat
      961 ctcagcgtaa agaatggcga gcataatagg accacccctc ttggtttcta ccacatggtt
     1021 gataaggtgg ttgccgggga ggaagaagtt ggatgtatta ggttcatact tatgaatggt
     1081 aaagattaca tggttaatca ccctgacctt ttccagtgcg gtgaggttgc tgtttatttc
     1141 gagccagata tccttttgaa gcttcttaat ctccaattca ataggctgga gcgataaatc
     1201 gggaaactgc agacgggcta ccagcactgc tccctctagt agattgttac tttccgaatc
     1261 tttccactgg gtgagagcgt tggcaaccgt gttaaactga atggtctgaa tcagattctc
     1321 cagcctttcc tgaaatatcc tgctgtccga cgacccccat gcaccctcaa gcagcggaac
     1381 aacggtaatg ccctgcgtga tgattttttc cgacacggcg ttgaaaatct cggggtcggg
     1441 atcgtcaagc agggtaatga gggagtgtat gtagcttttg tccaccactt gcagtgtgtt
     1501 aaatggatgc aagcctgttc agcgtgttct ctatcagatt tttcatggcc agctggtagt
     1561 caaccttggc atcctttact acccggttgg caatgatggt gcagatggta acggaatcat
     1621 ggccaaggag cagcgaaagc ccgttgattg ccgagctttc catttcaaag ttggtaatct
     1681 tatagccctt atacctgaaa tctacaattt tatcgttgag cgttggatcg gccaacggga
     1741 gtcgcagtac tcgtccctgc gggccgtaga aaccaggcgc cgaaatggta actcctttca
     1801 cggttatacc ggcaaatagc tcaaccagct tgcgggacga atggcagaag tatggtttag
     1861 cacaaagcgg attccactgg gtatgcttca ggaaggcctg ctccatttcc aggtcgcata
     1921 ttccattccg gttggcatag aagttcaaca gtccatcgaa accaatgctt atctcggtaa
     1981 gcaccgagct gccgagctct atctcgggtt gaagcgcccc cgatgttccc agcctcacca
     2041 gggtgaactt ggtgtggttg gccttgactg ttcggctggc caggtcaata ttggccaggg
     2101 catccagctc gttcaccact atgtcgatgt tgtcggttcc tattcctgtt gaaaccacgc
     2161 ttatccgctt gcctttgtat gttccggtgc aggttttgaa ctcccggttc gatacggtga
     2221 gctcaacccg gtcaaagtac gaggaaaccg tttcaaccct acccggatca ccaaccagaa
     2281 ttacagtatc tgccagctgt tcgggtttaa ggtgtaggtg gaagacgctg ccatcctggt
     2341 tcagtatcaa ctccgacgac tcaattactc tcatagctac tagttttgct gccaaaaata
     2401 ctaaaaatcg ttcattttat ggtctaatag ctacaaagac ttaacgtttt ttttacatgt
     2461 catccattgc ataaaaaatt gaactattcc tggcatgcac attccatttt gtatcaaaaa
     2521 atgccagtat ctatgttatc aacacctaaa tccttaggac ggttagcagc gtgttgatag
     2581 cctgtttgca gcttgtttat aattctaatg tccttgtggt ggtgcggggt tcattgggca
//
LOCUS       DAOOQX010000240         2639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_47104_length_2639_cov_3.402864, whole genome shotgun sequence.
ACCESSION   DAOOQX010000240 DAOOQX010000000
VERSION     DAOOQX010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47104_length_2639_cov_3.402864"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLX53_04780"
     CDS             complement(<1..278)
                     /locus_tag="PLX53_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOY72428.1"
                     /translation="MEVAKAALRSEIKRQKELVGAERKMDHARKVFGVVESMEEFKQA
                     KTVLAFWSLPDEIYTHDFIMKWAGLKHIALPVMVGDYLELRLFSSKENL"
     gene            complement(321..>2639)
                     /locus_tag="PLX53_04785"
     CDS             complement(321..>2639)
                     /locus_tag="PLX53_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72429.1"
                     /translation="HYRAHFLADRKTYSYNKWLLGSYKNYIPNNYSLGYQLVSFGNFK
                     YGINTWPKVVNHVAKYPFTAIPFYFGVKQNTGLPARKLADSTFAYLIGRWKADTVDRY
                     LSAVNHPVRMPKSYTSYLYPYKLNDSTMLAYRTSLNDIPAFVEINLNSQKVKELCYPG
                     YLLGRPTYSDSLIVWAEYQPHTRWEYEDFGRIYGLRIKDAQKIELSGKGKYFSPAINP
                     DDCSVLCISYSPDGNFKLVSIGKNREERELLSFPSGYEVQELSFDRNTRMLYALVVTQ
                     QGKEIVSVDINKSFNVVYGPTFDDVNSIYAFDSRVIFSSTDGDVENIYLHNNLTDSTV
                     KIYNSRYSSRYCSVTKNHELIFSDYTPDGYTIGTSTIDLDNQKNDLISNRVNNSYPNF
                     TLIGKGSVVVDTLQISDSVFSSKPYKGAGTLFNVHSWMPFFVNIDGTEVTSINPGVML
                     LSQNLTGTLTSSIGYGYGNSHLFHTDVTYYGFWPVLSVRFNLYDEDAFLYRVTENPVF
                     PNKNRWEAKLNVYLPLQLANSSYQTYLQPFSDLTLSNDYLFSSNDSSYHSGIGTVNSG
                     FYFHRLRNMAHRDLQPRMGLVFRLNLVNAPFNKNSLGSLQAANLKIFLPGFLANQGLK
                     FSAAWQNQNLKRYYLGNEISFPRGFSENVSKKYLGASADYIFPFITPDLSLGALAYIK
                     RISVNLFYDYARNSYLWVNNNNEIAVHDNLTSCGFEIITDFHLFRFRFPFRLVVQQAF
                     TGSNWNPYTNVSLTLDVYGEFWGNKVNRTLGY"
BASE COUNT          762 a          548 c          553 g          776 t
ORIGIN      
        1 agattctcct tactgctgaa aagccttaac tccagatagt ctccaaccat aacgggcaac
       61 gcaatatgct ttaaacctgc ccatttcata ataaaatcgt gggtatatat ttcgtcggga
      121 agcgaccaga acgccagaac tgttttcgcc tgcttaaact cctccataga ttcaacgaca
      181 ccaaaaacct ttcttgcatg atccattttt ctctcagcac caactagttc cttttgccgt
      241 tttatttcac ttcgtaatgc tgcttttgct acttccattt ttattagttg cttttgatat
      301 tgtggttgta tatacgtaag ctagtacccc aaggttctat taactttgtt gccccaaaat
      361 tctccgtaaa catccaatgt taagctaaca tttgtgtaag ggttccaatt gctccctgta
      421 aaggcttgct gaacaaccag gcggaacggg aatcgaaatc taaacaggtg gaagtcggtg
      481 atgatctcga aaccacacga ggtgaggtta tcgtgcacag caatttcatt gttgttgttc
      541 acccataggt aggaattacg ggcatagtcg taaaataggt tcaccgaaat tcgtttgatg
      601 taagccagtg cccccaagga aaggtcgggg gttataaaag gaaaaatata atcggctgat
      661 gctccaaggt attttttcga tacattttct gaaaacccac ggggaaagct aatctcgttt
      721 cccaaatagt accgtttcag gttttggttt tgccatgcag ctgagaattt caagccttgg
      781 ttcgccagga atccgggtag gaatatttta aggtttgccg cctgcaacga cccaagggag
      841 tttttgttaa agggtgcgtt tacaaggtta agcctgaaaa ccagacccat acggggttgt
      901 aggtcacgat gtgccatgtt ccgtagccta tgaaagtaga acccgctgtt gaccgtgcca
      961 ataccgctat ggtagctcga gtcgttggag gaaaaaagat aatcattcga cagggttagg
     1021 tcagaaaagg gttgcaggta ggtttggtag ctgctgttgg ccagctgtaa gggtaggtaa
     1081 acgtttagct ttgcctccca tctattttta ttgggaaaaa caggattttc tgtaactcta
     1141 taaagaaacg catcctcgtc gtataggtta aacctcaccg acaaaaccgg ccaaaatcca
     1201 tagtaggtta catcggtatg aaaaaggtga gaatttccgt atccatagcc aatactggaa
     1261 gtaagggttc cggtaaggtt ttgagaaagt agcattactc ccggattaat tgaggtaacc
     1321 tcagtcccgt ctatgttgac aaaaaaaggc atccagctgt gcacgttaaa gagtgttccc
     1381 gcccccttgt agggttttga gctaaatact gaatcactaa tttgaagggt atcaaccaca
     1441 acagaaccct taccaattaa tgtaaaattt ggatagctat tatttaccct attgcttatc
     1501 aaatcatttt tctgattatc taaatcaata gttgatgttc ctatcgtata accatcggga
     1561 gtatagtcag aaaaaataag ctcatggttt ttagtaacgg aacaatacct agaactatat
     1621 cttgaattat aaatttttac tgtgctgtct gttaagttat tatgcagata tatattttct
     1681 acatcgccat ccgtagagct aaaaatcacc ctgctgtcaa aggcgtatat cgagttaaca
     1741 tcatcaaaag taggtccgta aacaacattg aatgatttgt ttatatctac ggaaacaatt
     1801 tccttgccct gctgggtaac tacaagtgca tatagcattc gggtatttct atcgaacgaa
     1861 agctcctgaa cttcgtatcc acttggaaaa ctcagcaatt ctcgctcctc acggtttttt
     1921 ccaatggaaa ccagcttaaa gttaccatca ggactgtagg aaatgcacag aactgaacag
     1981 tcgtctgggt taatggctgg cgaaaagtat ttgcctttcc cacttagttc aattttttgg
     2041 gcgtccttta tccttaaacc ataaatcctg ccaaagtctt cgtactccca tcgggtatgt
     2101 ggctgatatt cagcccaaac aataaggctg tccgaatagg ttgggcgacc aaggagatac
     2161 cctggatagc atagctcctt aaccttttgg ctgttcaggt ttatttcaac aaacgctggt
     2221 atgtcgttta gtgaggttcg gtaggccagc atggtggagt cattaagctt gtaggggtag
     2281 aggtagctgg tatatgactt aggcattcta acaggatggt taacggctga aagataccgg
     2341 tcaaccgtat cggccttcca tctaccaatc agatatgcaa aggtgctatc ggccagcttc
     2401 ctagccggaa gtcctgtatt ttgcttcaca ccaaaataaa atggaatagc tgtaaaagga
     2461 tacttggcca catggtttac aacctttggc caagtattaa tcccatactt aaaattacca
     2521 aaactaacca gctgataacc taagctatag ttattgggaa tataattttt ataggatccc
     2581 aacagccact tgttatatga gtaggtcttt ctatcggcca aaaagtgagc acggtaatg
//
LOCUS       DAOOQX010000241         2633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_47235_length_2633_cov_4.358805, whole genome shotgun sequence.
ACCESSION   DAOOQX010000241 DAOOQX010000000
VERSION     DAOOQX010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47235_length_2633_cov_4.358805"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLX53_04790"
     CDS             complement(<1..390)
                     /locus_tag="PLX53_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72430.1"
                     /translation="MLLFVFSCFLLFTPRVSIGGQVYEQTVNLQDTTRLPYPIKSENF
                     LTPATVRSPLFLNQPSNVTEVVEYDPTTKKYTVYQRVGGYNVGLPRVMSDEEYQNYRV
                     EQSMREYGRQKQTGETVGKGDGILPRIQ"
     gene            complement(442..1026)
                     /gene="ruvA"
                     /locus_tag="PLX53_04795"
     CDS             complement(442..1026)
                     /gene="ruvA"
                     /locus_tag="PLX53_04795"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOY72431.1"
                     /translation="MYDYIVGKLAELTPTHAVVEVGGLGYHVNISLQTYSAINGMNEV
                     KLYLHQVIREDAHLLYGFFEAKEREIFRLLISVSGVGASTARLILSSLNADELQAAIL
                     TENINLIKQVKGIGLKTAQRIVVDLKDKVVGDDFASKQIMPAAHNTSRDEALSALVML
                     GFSKSATEKVLDQIIREGGELTVEGLIKSALKRL"
     gene            complement(1153..2205)
                     /gene="pdxA"
                     /locus_tag="PLX53_04800"
     CDS             complement(1153..2205)
                     /gene="pdxA"
                     /locus_tag="PLX53_04800"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOY72432.1"
                     /translation="MSDEKIRVGITQGDINGIGYEVIIKTFMDPRMVELCTPVVYGSP
                     KVAAYHRKALNVENFSFNHIRDAAEANPKRVNIINCMDDEVRVELGKSTEEGGKGSLL
                     ALEAVMPDLKAGKIDVLITAPINKANIQSAKFSFPGHTEYLASSFNAKEFLMLMVGDL
                     MRVGVVTGHVPLSKVSSLITRDTVLGKIRLLNQTLVRDFAIRRPRIAVLGLNPHAGDA
                     GLLGGEEESAIIPAIKQAADEGIAALGPYPADGLFGSDGFTRFDAILAMYHDQGLTPF
                     KAICFDSGVNYTAGLPVIRTSPDHGTAYDLAGQNLANPNSFRNALYLAIDIFNNRKLH
                     DQLTTNPLSVSSSENG"
BASE COUNT          665 a          668 c          593 g          707 t
ORIGIN      
        1 ctgaattcgt ggcagaatgc catcgccctt tccaactgtt tcgccggttt gcttctgccg
       61 cccgtactcc cgcatggact gctccacccg gtagttctgg tactcctcgt cgctcatcac
      121 acgaggcaaa cctacgttat acccgccaac tcgctggtaa acagtatatt tctttgtggt
      181 ggggtcgtac tccaccacct cggtaacatt cgatggctgg tttagaaaca ggggtgaacg
      241 caccgttgcc ggtgttaaaa aattttcaga ttttattggg tagggtagcc tggtggtatc
      301 ctgcaggtta accgtttgct catacacttg cccaccgatg gatacacgcg gggtgaacag
      361 cagaaagcat gaaaatacaa acaacagcaa ggaacctatt ttaacgcgtg ccaaagtcaa
      421 tgcctctcca gtgataaagt tctacaagcg ctttaatgcc gatttaatca gcccctcgac
      481 agtcaactcc ccgccttccc tgataatttg atcgagcacc ttttcggtgg cagacttgga
      541 aaaaccaagc ataaccaatg cagataacgc ttcatccctc gaagtattgt gtgctgcagg
      601 cataatttgt ttggaagcaa agtcatcgcc caccacttta tcctttaggt ccaccacaat
      661 gcgctgggct gttttcaggc caatgccctt aacctgcttt atcaggttaa tattttcggt
      721 aagaatggcc gcctgaagct catcggcgtt aagcgacgaa agaattaaac gggctgtgct
      781 cgctcctaca cctgaaacgc tgataagcag cctgaaaatt tcccgctcct ttgcttcgaa
      841 aaagccgtaa agcaaatggg catcctccct tatcacctga tgaaggtaga gctttacctc
      901 attcataccg ttgatggccg agtatgtctg cagcgaaatg tttacatggt atcctaaccc
      961 tccaacttcg accacagcgt gggtgggcgt aagttcggcc agcttgccta caatatagtc
     1021 gtacatttcc tttacgttgg tttgaattaa gtatgggcta ttaggctgat ttgtttggcg
     1081 ttatgagcca ctactttcag gtagagctga tataaggatt tggtggcaag cacacctcaa
     1141 cccattgcta aactaaccat tttccgagct agaaacgctc aaagggttgg tagtgagctg
     1201 gtcatgcagt tttcggttgt tgaatatgtc tatggccagg tatagggcat ttctaaacga
     1261 gttggggtta gccaagtttt gcccggccaa atcgtaggct gttccatgat cgggtgaagt
     1321 tcggataacc ggtaggccgg ccgtgtagtt taccccgctg tcgaagcaaa ttgccttgaa
     1381 tggggtaagt ccctggtcgt ggtacatggc cagaatagcg tcaaaccgag taaaaccatc
     1441 ggaaccaaac aggccatcgg ctgggtatgg gcctaacgct gcaattccct catcagcagc
     1501 ctgctttatg gcaggtataa ttgccgattc ctcctcacct cccagcaatc cggcatcacc
     1561 tgcatgggga ttaagcccca acacagcaat acgtggcctg cggatggcaa aatcccttac
     1621 cagcgtttgg tttagcaaac gaatttttcc cagtaccgta tcacgggtaa ttaacgacga
     1681 gactttcgaa agaggcacgt ggccggttac cacgcctacc ctcattaaat cgccaaccat
     1741 aagcataagg aattccttgg cattaaacga gcttgccaga tactcggtgt gtcccgggaa
     1801 gctgaatttt gcactttgaa tattggcctt gttaatgggt gcggtaatca gcacatcaat
     1861 tttaccggcc ttcaaatcgg gcataacagc ctccaatgca agcagcgagc ccttacctcc
     1921 ttcctcggtt gattttccga gctcaaccct cacctcgtca tccatgcagt taatgatgtt
     1981 tacccttttc ggattggcct cagccgcatc acgaatgtgg ttaaagctga aattttcaac
     2041 attcaacgct ttccggtggt aggctgcaac cttaggcgat ccgtaaacaa ccggagtgca
     2101 gagttcaacc atccgggggt ccataaaggt tttaattatc acctcgtagc ctataccgtt
     2161 aatgtcgccc tgggtaattc ctacccttat tttctcatcg ctcatgtagt ttctttcagt
     2221 tagtagggtt aaaacttaca aacgtacttg ataattttca attaaccaca gactaaagaa
     2281 aaacttttac aggaacacgg gtaatgctga ttaagcttac actcaacaga tttgttcctg
     2341 ctattcagta gctacaaaat tatttgtgcg ccagctcttc aaaattttaa actatacaga
     2401 agaaagctca gaacctgtcc gccttgcggc ggaacttgtc cggctcctga cggattcacc
     2461 cttcgactgg tgttacagtg agcctgccga actgctcact ataacatttg tcgaactgct
     2521 cagggttcag acttgtccac cttacagtgg cttacggatt aaagaacttg tccgatgttt
     2581 taagccccga aggggcgtaa taaatagcaa ggggcgcagc cccttgtatg gag
//
LOCUS       DAOOQX010000242         2630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_47320_length_2630_cov_2.460971, whole genome shotgun sequence.
ACCESSION   DAOOQX010000242 DAOOQX010000000
VERSION     DAOOQX010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47320_length_2630_cov_2.460971"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLX53_04805"
     CDS             complement(<1..627)
                     /locus_tag="PLX53_04805"
                     /inference="COORDINATES: protein motif:HMM:NF020653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrinase N-terminal
                     domain-containing protein"
                     /protein_id="HOY72433.1"
                     /translation="MKKPLILMLMLIAACSMNVNSQNIKRVEPPCWWVGMKNPRLQLM
                     IYGSNINQAQVGISDSRVTLESVDKTENPNYLFLNLAINENAEPGAFNITFKFPKGKP
                     RVVAYELKERKQGSSERIGFNNSDVIYLVFPDRFSNGNPANDSIKGFSDKYNRNDPYG
                     RHGGDIQGIINHLDYFNQLGVTALWLNPILENNQPSTSYHGYAITDFYK"
     gene            complement(764..1825)
                     /gene="fbaA"
                     /locus_tag="PLX53_04810"
     CDS             complement(764..1825)
                     /gene="fbaA"
                     /locus_tag="PLX53_04810"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOY72434.1"
                     /translation="MHFKPGIITGNELNKLFEYAKQEGFALPAVNVTGTNTMNAVMEA
                     AHEVGSPVIIQFSNSGASFISGKSISNDGQRASILGAVSGAKHVHLLAEAYGVPVVLH
                     TDHAAKKLLLWIDGLLDMGEKYYKETGAPLFSSHMLDLSEEPLHENIEICKRYLERMS
                     RMGMTLELELGVTGGEEDGVDNTGVDNSRLYTQPDDVAYAYEELMKVSPNFTIAASFG
                     NVHGVYKPGNVVLNPIILKNSQELISRKFGKGSKPVNFVFHGGSGSEKEKIAEAISYG
                     VVKMNIDTDLQWAFWDGIKNYYQSKKEYLQGQIGNPEGEDSPNKKYYDPRVWLRKGEE
                     SVINRLKEAFADLNALNRA"
     gene            complement(1856..>2630)
                     /locus_tag="PLX53_04815"
     CDS             complement(1856..>2630)
                     /locus_tag="PLX53_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HOY72435.1"
                     /translation="KSTNNAKYQELCSRIGFDEVNEIARWKEIRSNIFLPEDKKLGIF
                     LQQEGYLDKEQILVKNLDPKERPINQKWSWDRILRSCFIKQADVLQGLYFLEDRFDTE
                     TIKRNYEFYENRTVHESSLSPCVHSVLASRIGNVEKAYELYLRTARLDLDDYNNEVKE
                     GLHITSMAGTWISIVEGFGGMRVRSNMLTFNPLIPQNWKSFSFKVWFRGNVLKIRVTN
                     SELSLFNEVGSNITVKIYGKDQLIPEGGAITIAVQKQVY"
BASE COUNT          693 a          600 c          542 g          795 t
ORIGIN      
        1 cttgtagaag tcggtaatgg catatccatg gtaggaagtt gaaggctggt tattctccaa
       61 gatcggattt agccaaaggg cagtaacgcc tagctgatta aaatagtcta agtggttaat
      121 aattccctgt atgtctccac catgcctgcc ataaggatcg ttccggttgt acttatcgga
      181 gaaaccctta attgagtcgt tagcgggatt gccgtttgaa aagcggtcag gaaaaacgag
      241 gtagattaca tcggaattgt taaagcctat cctctccgac gaaccctgct tacgttcttt
      301 caactcgtag gcaaccaccc ttggttttcc ctttggaaat ttaaaggtaa tattgaaagc
      361 cccaggttcg gcattctcat taatagcaag attgagaaac aggtagttgg ggttttcagt
      421 cttgtcaacg ctttcgagtg taacccggct gtccgagatt cctacctggg cttggttgat
      481 gttactacca taaatcatca gctggagcct ggggttcttc atgcccaccc accagcatgg
      541 aggttcaacc cttttgatat tctgcgagtt gacattcatt gaacatgctg caattagcat
      601 caacatcaaa attaatggtt tcttcatagt aataatgctt ttaatgttaa acaaaaaggg
      661 ttaaccccag aggattaacc ccaataaatt acttatcaag ttaaaaagtt gactctcaag
      721 ttacagaatg aatactggta tcctgtgaag catccgttca tggttaagca cgattaagcg
      781 cattcaaatc ggcaaaagcc tcctttaggc gattaatcac cgactcctcc ccttttcgga
      841 gccatacacg tgggtcgtag tactttttgt taggcgaatc ctccccttcg ggatttccaa
      901 tttgcccctg gaggtactcc tttttagact ggtagtaatt tttaatacca tcccagaaag
      961 cccattgaag atcggtatcg atattcattt ttaccacacc gtagctaatg gcttcagcaa
     1021 ttttttcctt ttcggaaccg cttccaccgt ggaatacaaa atttaccggt ttgctaccct
     1081 taccaaattt cctgctgatt agctcctgag aattctttag tattatagga tttagcacta
     1141 cgtttccggg tttataaacg ccatgtacgt tgccaaacga agcagcaata gtaaagttgg
     1201 gacttacttt catcaactct tcgtaggcgt aggcaacatc atcaggttgg gtgtagagcc
     1261 gtgagttgtc caccccggta ttgtcaacac catcctcctc gccgccagtt actcccagct
     1321 ctaattcaag ggtcatcccc attctactca ttcgttcgag atagcgctta caaatctcta
     1381 tattttcatg aaggggttct tcggataggt cgagcatgtg ggagctaaat agaggagcac
     1441 cagtttcctt atagtacttt tcgcccatat caagaagtcc atcaatccag aggagtaact
     1501 ttttagctgc atgatcggtg tgtaaaacta ctggtacccc gtaggcttct gcaagcaagt
     1561 gtacatgctt tgcgccgcta acagcgccaa gtatgctggc gcgttgtcca tcgttggaaa
     1621 ttgattttcc tgagatgaac gatgcaccgc tgtttgaaaa ctgaatgatt acaggtgatc
     1681 caacttcatg agctgcttcc attacagcat tcatagtgtt tgtgccggta acattaacag
     1741 ccggcagagc aaacccttcc tgcttggcgt attcgaatag tttattcaac tcgtttccgg
     1801 ttataatacc cggtttaaaa tgcatattcc ccattattta gctttattag atgttttagt
     1861 aaacttgttt ttgaacggca atggtaatag cgcccccttc aggaattagc tggtccttcc
     1921 cataaatttt aacagtgatg ttgctaccca cttcgttgaa gaggcttaac tccgagtttg
     1981 taacccttat tttgaggacg ttaccacgaa accaaacttt aaagctgaat gacttccagt
     2041 tttgtggaat taaaggatta aaagtgagca tattactgcg aacccgcatg ccaccaaatc
     2101 cttccacaat tgaaatccac gtaccggcca tactggtaat atgcaggcct tcctttacct
     2161 cgttgttgta atcatccaaa tcgaggcggg ctgttcgcag gtagagctca taggcttttt
     2221 caacgttacc aattctggaa gccagcacac tatggacgca gggtgaaagg gaagactcgt
     2281 gaactgttct attttcgtaa aattcgtagt ttcgctttat ggtttcggtg tcaaatctat
     2341 cctcaagaaa atataggcct tgcagaacgt cggcctgctt aataaagcag gaacgtaaaa
     2401 tacggtccca gctccatttc tggtttattg gccgttcctt gggatctaaa ttcttaacca
     2461 gtatctgctc cttatccagg taaccttcct gctggagaaa gattcccaat tttttatctt
     2521 caggaaggaa gatgttagag cgaatttcct tccagcgggc aatctcattc acctcatcaa
     2581 aaccaatcct gctgcaaagc tcttggtact tggcattgtt ggttgacttt
//
LOCUS       DAOOQX010000243         2627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_47390_length_2627_cov_3.353033, whole genome shotgun sequence.
ACCESSION   DAOOQX010000243 DAOOQX010000000
VERSION     DAOOQX010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47390_length_2627_cov_3.353033"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLX53_04820"
     CDS             complement(<1..258)
                     /locus_tag="PLX53_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOY72436.1"
                     /translation="MRNDKINKNKIVNDPVHGFISINYPLIFDIIEHPYFQRLRGIKQ
                     LGLTYLVYPGANHSRLQHALGAMHLLDTAIDTLRSKGFVISE"
     gene            complement(414..983)
                     /gene="ruvC"
                     /locus_tag="PLX53_04825"
     CDS             complement(414..983)
                     /gene="ruvC"
                     /locus_tag="PLX53_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOY72437.1"
                     /translation="MTNSTPAEKIILGIDPGTNILGYGIVRCSGKNDLKLVVLGVIEL
                     KKYEDHYLKLKAIYDRVQQLADQYLPDEVAIEAPFYGKNVQSMLKLGRAQGVAMAAVL
                     SRSIPIFEYAPRKIKQAITGSGSASKEQVAAMLKSILGEKDFSQEYLDATDGLAAAVC
                     HFFQGATIGAGRKASSWSDFVRQNPGRVK"
     gene            complement(1092..2351)
                     /locus_tag="PLX53_04830"
     CDS             complement(1092..2351)
                     /locus_tag="PLX53_04830"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOY72438.1"
                     /translation="MNWHKILSLAVIAAMVLSSCDNEDEQKVNVNQEVYNLMDDMYYW
                     YDQMPEVDPNAYKDPVELVNALRVYPPDRWSYVTTKAEFDAYYNQGAYVGFGFGSGFS
                     ANNELYITFVFKNSPLAAMGIDRGWQIISIDGQTPTSENFSSLIGPSEVGVSKKFLFK
                     SPEGSTMEQIFTKSQIEMNSVLMDSIYTTGTFRVGYFVLESFIGPTEAELTGLFSKFK
                     EEGINELVVDLRYNGGGEVGVAKYLASLIAGSSAFGKVFGTYYHNDKHQGDNSSITFT
                     YEDNSIPLNRVVFITTSGTASASELVINGLKPHIEVSLVGSKTHGKPVGMYSFEFRDP
                     SIDWVIVPICFSIRNSNNEGDYFDGIPVDVSAADDITHPFGSMDEESLNAAFTHLGVT
                     AKGYTRKAAVGVQPFVGKGLQGEIGAW"
     gene            complement(2445..>2627)
                     /locus_tag="PLX53_04835"
     CDS             complement(2445..>2627)
                     /locus_tag="PLX53_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005682062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOY72439.1"
                     /translation="STAKLGNFSYLVPFVALIFISLILNEKIIWTTVAGLICIIGAIL
                     FQQYLGRKLDNEGQKP"
BASE COUNT          723 a          630 c          541 g          733 t
ORIGIN      
        1 ctcagaaatc acaaacccct tgctgcgaag agtatctata gccgtatcga ggaggtgcat
       61 cgcaccaagg gcatgctgta aacggctatg atttgccccg ggataaacca ggtaggttag
      121 gccaagctgc tttattcctc taagcctttg aaagtaaggg tgctcaatga tatcaaatat
      181 cagcgggtag ttaatgctga taaaaccatg aaccgggtcg tttacaattt tgttcttgtt
      241 gattttgtca tttctcatat atggtgaacc aaaacttgtt gtcgttatca aaaaaagcca
      301 gtaacattgt gtgaagacaa atttgccgag taattagagg gctcgattgt agaaatactc
      361 cgtgttttct acatgaattt tcattcaacc tgatagccag acataacttc catttatttt
      421 accctacccg ggttttggcg cacaaaatcg ctccagcttg atgcttttcg accggcaccg
      481 atggtcgcac cctgaaagaa gtggcatacg gcagcggcca atccatcggt agcatcgagg
      541 tactcctgcg agaaatcctt ttccccaaga atggatttca gcatggccgc cacctgctcc
      601 ttcgaagcgc ttcccgagcc ggttatagcc tgctttattt ttcgcggtgc gtactcaaaa
      661 attggaattg agcgagaaag aacagctgcc atggcaaccc cctgagcccg gccaagcttt
      721 agcatactct gaacattctt accgtaaaaa ggggcttcaa tggccacctc gtcgggcagg
      781 tactggtcgg ccagctgctg tacccggtcg taaatggctt tcaacttcag gtagtgatcc
      841 tcgtacttct taagctcaat aactccaaga acaaccagct ttaggtcgtt tttcccgcta
      901 cacctcacaa ttccgtaccc aagaatattt gtgcccggat cgattccaag aattatcttc
      961 tctgctggtg tggagttggt catgaattta ctttactcat aacaaacatt tataccaaaa
     1021 tggagacctc gtaaacctgt taaactggtt cgaaagcctg gcttatagcc tgtagttttg
     1081 tttagcacac cctaccatgc gccaatttcc ccttgtagtc ctttgcctac aaatggttga
     1141 acgccaacag cggctttacg tgtataacct tttgcagtta ctccaaggtg ggtaaaagcg
     1201 gcattcaggc tttcttcgtc catactacca aacggatggg taatatcatc ggcagcggaa
     1261 acatctacag gaatcccatc aaaataatcg ccctcgttat ttgagttccg gatagaaaag
     1321 catataggca caataaccca gtcaatgctg gggtctctaa actcaaacga gtacatccca
     1381 acaggtttgc catgcgtttt tgaaccaacc agcgatactt caatatgagg cttaagcccg
     1441 ttaatcacga gctcgctggc cgaagccgtt ccgcttgtag tgatgaaaac aacccggtta
     1501 aggggaatcg aattgtcctc gtaggtgaaa gttatggagg agttatcgcc ttggtgcttg
     1561 tcgttatggt agtaggttcc gaaaactttt ccaaaggcgc tgctgccggc aataagactt
     1621 gccaggtatt tggcaacacc tacctctcca ccgccgttgt acctcaagtc aactaccagc
     1681 tcgttaatgc cctcctcctt gaattttgag aaaagaccgg ttagctccgc ttcggttggg
     1741 ccaataaagc tttctaaaac aaaatagcct accctgaagg tgccggtggt ataaatcgaa
     1801 tccatcaaaa cggagttcat ttcaatctgg ctcttggtaa aaatctgctc catggtgcta
     1861 ccttcaggag atttaaataa gaatttcttc gatacaccta cctcggatgg gcctatcagg
     1921 ctagaaaagt tttcggaagt tggggtttgt ccatcaatag aaataatctg ccaacccctg
     1981 tcaattccca tagcggcaag cggggagttt ttaaaaacaa aggtgatgta aagttcgtta
     2041 ttcgcactaa accccgagcc aaaaccaaac cccacgtacg caccctggtt gtagtatgca
     2101 tcaaactcgg ctttagttgt aacatagctc cagcgatcgg gcggatatac tcgtaaggca
     2161 ttcactagct ccacagggtc tttgtaggca tttggatcaa cctctggcat ctggtcgtac
     2221 caatagtaca tgtcgtccat cagattataa acctcctggt tgacgttcac cttctgctca
     2281 tcttcgttat cacacgatga taaaaccatg gctgctataa ctgccagcga taaaatttta
     2341 tgccaattca tgggttattc tatttattag ggattgcaca tcattatcat acagcacata
     2401 aaacgtttct gaattaatta tgattaacct catgctaata ttacttaagg tttttgccct
     2461 tcattatcga gttttctgcc taaatactgc tggaagagaa ttgctccaat aatacaaatt
     2521 aatcccgcaa cagtggtcca aattatcttc tcgtttagta ttaagcttat aaaaatcaga
     2581 gcaacaaaag gcacaagata tgaaaagttt cccaactttg ccgtgct
//
LOCUS       DAOOQX010000244         2622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_47535_length_2622_cov_3.618621, whole genome shotgun sequence.
ACCESSION   DAOOQX010000244 DAOOQX010000000
VERSION     DAOOQX010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47535_length_2622_cov_3.618621"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PLX53_04840"
     CDS             <1..340
                     /locus_tag="PLX53_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HOY72440.1"
                     /translation="KFTRSRFLQNLLNTITTARATWNGHNVSGWKADIVAVNGYNENM
                     QYGGLDRELGERLVNYGLKGKQIRYSAACIHLDHPRPYKTQLTLDQNKQIRKDVRRNK
                     ITWTSNGIIK"
     gene            359..1207
                     /locus_tag="PLX53_04845"
     CDS             359..1207
                     /locus_tag="PLX53_04845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY72441.1"
                     /translation="MPRPVASLIISTYKNVEFLNAVIKSVFRQTDRRVEIIISEDGEC
                     EHTKEFVSELRSPLPLRHLTQPDVGWRKNKALNRAILASTTDYLIFIDGDCMLHPRFI
                     EFHLKFASDNCILAGKRVKLDPHTSALLLNDQLVVSQMNAYLLKNFTSLKRQKAKFIE
                     EGIFINPRGPFAFIPAIRAMHQLKGCNMSFSKKAIYSINGFDEDYINPAIGEDIDLTW
                     RFKMAGFRIKSLRNLAVQYHLHHKECWTEQVINHDLMLRKQKSGSVICSNGLIKTSLG
                     NNCCQE"
     gene            1219..1791
                     /locus_tag="PLX53_04850"
     CDS             1219..1791
                     /locus_tag="PLX53_04850"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOY72442.1"
                     /translation="MNVSNPSLLRAAETLRSGGIILYPTDTVWGIGCDATNPAAVDKI
                     FRLKQRDDSKSMIVLVDQPGRIPSYVDEVPEVAWDLVELADKPLTIIFQKAKNLAPNL
                     IAPDGSIGIRVVRHDFCQKLILQFGKPIVSTSANISGRPFPKNFSEVDEVIKSGVDFI
                     VPQTYDHPVSDKPSGIIALGDGGLVRIIRE"
     gene            2181..>2622
                     /locus_tag="PLX53_04855"
     CDS             2181..>2622
                     /locus_tag="PLX53_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72443.1"
                     /translation="MQTLTATPNMVGMAVLLGMLLVIGLLLYRNKQLKNNNKELLATI
                     RQLQTMNARQEEISATLTDKLNDTLQKLSELTECKTSFWVDLNHRMRTSLNGLLGFSQ
                     ALLMNNTTKKEKDAAAQQIQQLCNKIILLADEMVDLAKIEANQLE"
BASE COUNT          793 a          621 c          559 g          649 t
ORIGIN      
        1 taagtttacc cgcagcaggt ttctgcaaaa cctcctgaat accataacta ctgcccgtgc
       61 cacatggaat gggcataatg ttagcggctg gaaagcagat attgttgcag taaacggcta
      121 taatgagaac atgcagtacg ggggcttaga ccgtgaactt ggcgaaaggc ttgtcaatta
      181 cgggctaaaa ggaaagcaaa ttaggtacag cgctgcctgt attcacctgg accatccacg
      241 tccgtataaa acacaactta ccctggacca aaataaacaa atccgcaagg atgtaaggag
      301 aaataaaatt acctggacat ccaacggtat tatcaagtaa ctctatacat ccatatttat
      361 gcccagacca gttgcctccc tgattatttc aacctacaag aacgtagaat ttcttaatgc
      421 tgtcataaaa tcggtgttca ggcaaaccga cagacgagtt gaaattatta tttctgaaga
      481 tggggagtgt gaacatacta aagaatttgt ttcggagctt agatcacccc ttcctctcag
      541 gcacttaacc caacccgacg ttggatggcg aaaaaataaa gctttaaatc gtgccattct
      601 ggcttcgaca accgactacc taatttttat tgacggcgac tgcatgctac atccccgctt
      661 catagaattt cacctaaaat ttgcctccga caactgcatc cttgccggaa aacgggttaa
      721 acttgacccg cacacgtcgg cgttgctgct taacgaccag cttgtcgttt ctcagatgaa
      781 tgcctatttg ctgaaaaatt tcacttcgct caaaaggcaa aaggcaaaat tcattgagga
      841 gggtatattc attaatcccc gggggccatt tgcatttata ccggccatcc gggcaatgca
      901 ccagcttaaa gggtgcaata tgtctttttc caaaaaggcc atctattcca taaatggctt
      961 tgatgaggat tacataaacc cggcaattgg ggaggacatt gatctaacct ggaggtttaa
     1021 aatggcgggt tttcggatta aatccctaag aaatctggca gttcagtatc atcttcatca
     1081 taaggaatgc tggacagaac aggttatcaa ccatgacttg atgctcagga agcaaaagag
     1141 tgggtcggtg atctgttcaa acgggctaat caaaacttca ctggggaata attgttgtca
     1201 ggaatagagc cagaatgtat gaatgttagc aacccatctc ttcttcgggc tgccgagacc
     1261 ctccgttccg gaggcatcat cctctaccca accgatacgg tgtgggggat tggctgcgat
     1321 gccacaaacc ctgcagcggt tgacaaaata ttcaggttga aacagcgcga cgatagcaaa
     1381 agcatgattg tgctggttga ccaacccggc agaattccat cctacgttga cgaggtacct
     1441 gaagtagcct gggacctggt tgagctggcc gataaacccc tgaccatcat cttccaaaag
     1501 gcaaaaaatc tggcgcccaa cctcattgcc cccgacggaa gcatcggcat tagggtggta
     1561 aggcacgatt tctgccagaa gctaatcctg cagttcggaa agcccattgt gtcaacctct
     1621 gccaacatat ccggacggcc attccccaaa aacttctccg aagttgatga ggtgatcaaa
     1681 tcgggagtgg atttcattgt cccgcaaacc tacgaccatc ctgtatcgga caaaccttct
     1741 ggtattatag ccctgggcga tggcggactt gtccggatta tcagggagta gctacaccgt
     1801 tatgggcaga taatcaacta tataagccaa aaatcaggtc ttcttgccat cccgacagga
     1861 gtaaagaaaa gttaatctcc actaaatcaa gttctatcaa atttttgacg tgtttcgcct
     1921 aaaaaccaga tgggcgtttt gataatgact tgaacgaaag ttacttttgc ccacagctag
     1981 caccctaaaa accatatcca ttcgctccga atcatttagc aatcccctgt taatacctct
     2041 agcagtcaaa ctttatgttg acttataacc ccaaagttga atggtagcgg ctattcggcc
     2101 ttaaaataac agcaaatttc tcaacaattt taattgcaaa tggcgtatcc tgaatgatac
     2161 cggataaatt aaacatcctt ttgcaaacct taacggcaac ccccaacatg gtgggcatgg
     2221 ccgttttgtt gggaatgttg ctcgtgatag gccttctgct ctaccgcaac aaacaattaa
     2281 aaaataataa caaggagtta ctggcgacga taaggcagct tcaaaccatg aatgccaggc
     2341 aggaagaaat ttctgcaacc cttaccgaca agctgaatga taccctccaa aagttgagcg
     2401 aactaacgga gtgcaaaacc agcttttggg tcgatttgaa ccatagaatg cggacatccc
     2461 taaatgggct gcttggattt tcgcaagcac ttcttatgaa taacaccacc aagaaggaaa
     2521 aggatgctgc ggctcaacaa atccagcagc tttgcaataa aattatcctt ctggccgacg
     2581 aaatggttga cctagccaaa atagaagcca accaactcga ag
//
LOCUS       DAOOQX010000245         2622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_47545_length_2622_cov_2.688742, whole genome shotgun sequence.
ACCESSION   DAOOQX010000245 DAOOQX010000000
VERSION     DAOOQX010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47545_length_2622_cov_2.688742"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PLX53_04860"
     CDS             complement(<1..837)
                     /locus_tag="PLX53_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOY72444.1"
                     /translation="MEIYYLIILILLFVLAISDLMVGVANDAVNFLNSAIGSRVAARH
                     WILLVASVGVLVGAVFSSGMMEIAQKGVFRPEKFYMSEIMTLFMAVMLTDVIVLDFFN
                     TFGLPTSTTVSLVFELLGSAVAISIFKISENNESIADLGSYINTAKALAMISGIFISV
                     VIAFFIGTIVQYIARVLFTFNYTRSMKYVGSIWGGISLTAITYFIAMKGLNSTALVSP
                     DTLNFINQHTMVILLLSFTGWTIIFQLAISIFKWNILRFVVLAGTFALALSFAGNDLV
                     NFI"
     gene            complement(944..1699)
                     /locus_tag="PLX53_04865"
     CDS             complement(944..1699)
                     /locus_tag="PLX53_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOY72445.1"
                     /translation="MFTALIVDDELNSRQTLAYLLSHFCPTVRVIGEAEGVESAYTSI
                     QKLNPDIVFLDLQLADGNGFDLLRMFEEVHFMLIIVTAYQEYAIRAFKYCALDYLLKP
                     IDVNSLVDAVDRLQHSFSSQEANRKFQVLLSNTSACSKKCKKLVLKSLEGLRVVETDE
                     IVRCESHNNTTEFILTTEQGVMVSKTLKEYEELLAPEGFIRCHQSHLINKRHVKEVLR
                     FPSPTIRMDNGDEVPVSVRKRDILRAIAGKQNG"
     gene            complement(1702..2388)
                     /locus_tag="PLX53_04870"
     CDS             complement(1702..2388)
                     /locus_tag="PLX53_04870"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOY72446.1"
                     /translation="MVAVVLVLAYRRLKRKYLRLEQQLLLSQLNPHFVFNSLTAIQSY
                     VFRNEAHLASKYLASFAKLTRLILENSRSELCSIENEVATLEHYLELQSLRFSNRFDY
                     SIYVDEKIDADSTLIPPMLTQPFIENAIEHGFNGIEDRGRLTISYLYLDKHHVEIEVK
                     DNGVGIKRSEQANPASGKDHLSLATEITLERLKNIRHYKRLNIILTLVDTGSIDPKQH
                     GTLVKFKIPI"
BASE COUNT          786 a          576 c          583 g          677 t
ORIGIN      
        1 aataaagttt accaggtcgt taccggcaaa cgaaagggct agggcaaaag tacccgccaa
       61 cacaacaaac cttaggatat tccatttgaa gatggatatg gccagctgaa aaataatggt
      121 ccacccggta aagctcagga ggagaatcac catggtgtgc tggttaataa agttcagggt
      181 atccggcgaa accagggcgg tgctgttgag acccttcatg gcaataaagt aggtaatggc
      241 cgtaagggag attcctcccc aaatactgcc aacgtacttc atgctacggg tgtagttgaa
      301 ggtgaaaagc acccgggcaa tgtactgcac aattgtgccg ataaaaaatg ctattaccac
      361 cgatataaaa atccccgata tcatagccag cgccttggcc gtgttgatgt agctacctaa
      421 atcggcaata ctttcgttgt tttctgaaat cttaaaaatt gaaatggcaa ctgcggagcc
      481 aagcagctcg aaaaccagtg ataccgtagt ggaggtaggc aggccgaaag tattaaagaa
      541 gtctagcaca ataacgtcag taagcatcac agccatgaac aaggtcatga tctcgctcat
      601 gtaaaacttt tcgggacgga acaccccctt ctgggcaatc tccatcattc cgcttgaaaa
      661 aaccgcgcca accagcaccc caaccgaagc caccagcaga atccaatggc gtgcggcaac
      721 acgcgatccg atagccgaat tcagaaagtt taccgcatca ttggctacgc caaccattaa
      781 gtcggatatt gccagaacga acagtaaaat aaggattata aggtagtata tctccatggt
      841 taaggagtta acaaacagtt agcaaagata aaatagcccg ttaaaatggt tagccgtggg
      901 ggattacgct tttgttaaga cattgttaag tgaatattaa caattaccca ttttgtttgc
      961 cggctatcgc cctgagtata tccctttttc gaaccgaaac gggcacttca tcgccgttgt
     1021 ccatccttat ggtaggtgag ggaaatcgga gaacttcctt aacatgcctt ttgttgataa
     1081 ggtgcgattg atggcagcga ataaaaccct caggagctaa tagctcctca tactccttca
     1141 gagttttaga caccatgaca ccctgctcgg tagtaaggat gaactcggtg gtgttgttat
     1201 gcgactcaca acgtacaatt tcatcggttt ctacaacccg tagtccctct agggacttaa
     1261 gaacaagttt tttacatttt ttgctacagg cgcttgtgtt tgagagtagc acctgaaact
     1321 tacggtttgc ctcctgactc gaaaacgagt gttgcagcct gtccaccgca tcaaccaagc
     1381 tgttaacatc tataggcttc agcaggtagt cgagggcaca atacttgaat gccctaatgg
     1441 cgtattcctg gtaggctgta acaatgatga gcatgaaatg cacctcctca aacatacgaa
     1501 gaaggtcaaa accatttcca tcggcaagct gcaaatcgag aaaaactata tccgggttta
     1561 gcttttgaat ggaggtgtag gcgctttcaa ctccttcagc ttctccaata accctgactg
     1621 ttgggcaaaa atggcttagc aggtaggcaa gcgtctgcct tgagttcagc tcatcatcaa
     1681 ctataagggc ggtaaacatg gttaaatggg aattttaaat ttgaccagcg tgccatgttg
     1741 tttgggatca atactaccgg tgtcaacaag cgtcaagatt atattcagcc ttttatagtg
     1801 tctgatgttt ttaagcctct ccagggttat ttcagttgca agcgatagat ggtctttccc
     1861 agaagccggg tttgcttgct ccgacctttt aatccccaca ccattgtctt tgacttcaat
     1921 ttcaacatga tgcttatcga ggtagaggta gcttattgtt aatcttcccc tatcttctat
     1981 accattaaat ccatgctcga tggcattttc gataaatggc tgggtgagca tgggaggaat
     2041 tagcgtgctg tcggcatcaa ttttctcatc aacataaatg gagtagtcaa aacggttgct
     2101 gaaacgcagg gattgcagct cgaggtagtg ttcgagggtt gcaacctcat tttcaattga
     2161 gcatagctcg gagcgtgaat tttcgagtat taggcgggta agcttggcaa aactagccag
     2221 gtacttgctg gccagatggg cctcgttgcg gaaaacgtag ctctgaatag cagttagaga
     2281 attgaaaaca aaatgaggat ttagctgcga aagcaaaagc tgttgctcca ggcgaagata
     2341 cttgcgcttt agccgtctat aggccaaaac aagtacaaca gctaccatcg taatagctac
     2401 ggtaaaaatc tcaaaaaaaa ggcagccatt catgcttatc ctttaaccat tgccccaact
     2461 ggtaaatcaa ttacaactac aaaattagca ggtagcattt agtttccaaa aaaatttaaa
     2521 aatcacagat agactaattt acaacgaacc aataaaaaac ccctacgcag tgcaggggta
     2581 gcctaaaaaa ggaacaaaat aatgctattg cttggctttg tt
//
LOCUS       DAOOQX010000246         2616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_47700_length_2616_cov_3.429520, whole genome shotgun sequence.
ACCESSION   DAOOQX010000246 DAOOQX010000000
VERSION     DAOOQX010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47700_length_2616_cov_3.429520"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            454..918
                     /locus_tag="PLX53_04875"
     CDS             454..918
                     /locus_tag="PLX53_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOY72447.1"
                     /translation="MEMKDRIRLLLDAEKINSAKFAEAIEVQRSAVSHILAGRNNPSF
                     DIIQRIAQKFPKINLEWLITGKGSMYKIPVQRSIFDTDDSINQPGKTAPTANLTESRT
                     ITNVNNRGEGGKILSGRYEGADSEDTSKELTVQKIVIFYSDRTFVEYRPSIS"
     gene            961..2016
                     /locus_tag="PLX53_04880"
     CDS             961..2016
                     /locus_tag="PLX53_04880"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOY72448.1"
                     /translation="MYKSIFRKLLFIFPPETVHKLVVSLIKFFFKIPGARNAFRRIFS
                     NRNEACLEREVIGLKFSNPVGLAAGFDKNAEFFNEFSSFGFSFIEIGTVTPEPQPGNS
                     RPRLFRLLPDNALVNRMGLNNKGVNYVKSRLQHKENQGLIIGGNIGKNSSTPNSNAVS
                     DYIACFNELYSFVDYVAVNLSCPNVHNLSDLQNPDSVKLIIESLVNERAKFPSKKPIL
                     LKVSPDLSPEQLNQTIDAAEEAGVDGYIATNTTTGRNNLKTKKSELEKIGRGGLSGKP
                     LRDVSTEVIRFINQKLDGKKPIIGVGGIFTPMDALEKLEAGASLVQVYTGFIYEGPFI
                     VKRINKLLINKMFNTYI"
     gene            complement(2167..>2616)
                     /locus_tag="PLX53_04885"
     CDS             complement(2167..>2616)
                     /locus_tag="PLX53_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72449.1"
                     /translation="RLGNPLIGMSIKDILYYYDEKDQLGLFNKSPRDLINEIKEMYKL
                     YFSLSDKIKEDVEIKKDMDMRLNTYKERIKDVMDVITASHFAKIIDSKKIRDLIFSLD
                     SDALTWEHIRKKNWFLEAKEIANKNGFFHLEINFPFLLHNAFNPIVL"
BASE COUNT          831 a          466 c          502 g          817 t
ORIGIN      
        1 attgcttgtt tttgttgata tatcaacgct attttgtttg gttttataat aaactgataa
       61 tcttatgttt attaatatat gttaatctaa tattttataa agaaatgaat tgtggctgta
      121 ttttgaacag taaaatagtt aaaataacat tataaatacc tactatccag atatattgtt
      181 ctatttaaat aatgtttaaa tagaactgta ggagtaggta gcctaaagtg ctataaaata
      241 tcccgattca agcaggttaa gcgtggctaa aataagcgta gcttcatttt taggtataaa
      301 gaatagagtt taccccgcta ctatgggaac taactatttt taaaaacttc agccctaaat
      361 ttttattggt tacatatgta attgttttaa aattttgatt tgccggttat aaaatttaca
      421 tttgtatgct caacgttaaa atcagatcgc taaatggaga tgaaggatag aatacggtta
      481 ttgctggatg cagaaaaaat taactcggct aaatttgccg aagcaataga agtgcaaaga
      541 agcgctgttt cacacatact agcaggcaga aacaacccca gctttgatat tatccagcga
      601 atcgcccaaa agttccccaa aattaatctt gagtggttga ttaccggaaa gggaagcatg
      661 tacaaaatcc ccgttcaacg atcaattttt gatacggacg attcaattaa tcaaccaggt
      721 aaaacagccc ctacggcaaa tttgacagaa agtcgaacga ttacaaatgt aaacaacagg
      781 ggtgagggtg gaaaaatttt atccggtcga tatgaaggtg ccgattcgga ggatacctca
      841 aaagaactta cggtgcaaaa aattgttata ttttattctg accgcacgtt tgtggagtat
      901 aggccatcaa tatcctgaat tttctctttc aattttctta cttttgtaga aaatttggtc
      961 atgtacaaat ccatttttag gaagttgcta tttatttttc cacccgaaac cgttcacaag
     1021 ctggttgttt ccctcataaa attttttttc aaaattcctg gagcaagaaa tgcttttagg
     1081 agaatttttt ccaatagaaa tgaggcatgt ttagaaagag aagtaatagg cttaaaattt
     1141 tctaacccgg taggacttgc tgctggattc gataaaaatg ccgaattttt taacgaattt
     1201 tcttcatttg gattttcgtt tatcgaaatc ggcacggtta cgccagaacc ccaacccggg
     1261 aacagcagac ctcgcctttt tagactttta cccgataatg cgttggttaa ccgaatgggg
     1321 ctaaacaata agggagttaa ctatgttaag tcgagattgc agcacaagga aaaccaaggt
     1381 ttaattattg gtggaaatat tggaaagaac tcatctacgc ctaatagtaa tgccgtttcc
     1441 gactacattg catgctttaa tgagctttac tcatttgttg actatgttgc cgttaacctt
     1501 agctgcccga atgttcataa cctcagcgac ctacaaaatc ccgatagcgt gaagctaatt
     1561 attgaatcgc tggttaatga aagggcaaaa tttccttcga aaaagcctat tctcctcaag
     1621 gtctcgcccg acttgagccc tgaacagctt aaccaaacaa ttgatgcagc tgaagaggct
     1681 ggtgtggatg gctatatcgc caccaatacc actactggac gtaacaacct taaaactaaa
     1741 aaaagcgaac tcgagaagat tgggagagga ggacttagcg gcaagccgct ccgcgatgtt
     1801 tcaactgagg ttatccgctt cataaaccaa aaacttgacg ggaaaaagcc gataattggc
     1861 gttgggggta ttttcacccc aatggatgcg ttggaaaaac ttgaagctgg agcatcgctg
     1921 gttcaagtat acacaggttt catatatgag ggtccattta ttgttaagag aataaataag
     1981 ctattgataa ataaaatgtt taacacatat atataaatag aaatggctaa acttgttgat
     2041 tgcaggggaa tggtctgccc ccaacccctg gttgaaacca ggcgggaaat caagaaatct
     2101 aatcctggtg aggccataac ggtggtggtg gataatagca cttcacttaa aaatatcctc
     2161 cgttttttag aggacaatgg ggttgaatgc gttgtgaagc aagaagggaa aattgatctc
     2221 caaatgaaaa aatccatttt tgttggcaat ctcttttgct tctagaaacc agtttttttt
     2281 cctgatatgc tcccatgtca aagcgtcgct gtcaaggctg aatatcaaat cacggatctt
     2341 tttgctgtca ataatctttg caaaatggga cgcagtaatt acatccatta catcctttat
     2401 tctttccttg tatgtattta atctcatgtc catatctttt tttatttcga catcttcttt
     2461 tattttatca gatagggaaa aatatagttt atacatttcc ttgatttcgt tgatcaaatc
     2521 cctcggactc ttattgaaaa gaccaagttg gtccttttca tcataatagt ataatatatc
     2581 tttgatggac atgccgatga gcggattccc aagtct
//
LOCUS       DAOOQX010000247         2608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_47906_length_2608_cov_2.766157, whole genome shotgun sequence.
ACCESSION   DAOOQX010000247 DAOOQX010000000
VERSION     DAOOQX010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47906_length_2608_cov_2.766157"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLX53_04890"
     CDS             complement(<1..418)
                     /locus_tag="PLX53_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOY72450.1"
                     /translation="MHLKHLSAINFKSFRQVELELNPKINCFIGNNGQGKTNLLDAIY
                     YLSMCKSAFNSSDRQVLRHGEQFFVIEGSYQRVSSNEDIYCGFKLDEGKIFRRNGKTY
                     DRLADHIGFIPIVMVSPTDSALIDESGEERRKYMNGV"
     gene            544..1236
                     /locus_tag="PLX53_04895"
     CDS             544..1236
                     /locus_tag="PLX53_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY72451.1"
                     /translation="MAKNNKNAQPDTVESIDNALSQTERFIEQNQKSLTIIIVAILVI
                     ISLYFGYKRFYLVPKEDEARSQIFQAEYYFEKDSFKLALNGNENDLGFVDIADNYSMT
                     KTGNLACYYAGICYRQLGDFNKAIEYLKKFDAGDIMVTPIAYGAIGDCYVELKQLDEG
                     VKFYIKAAEYSDNDFTSPFFYKKAGLIYEVMNNNGDALKIYQTIKDKYPKSNEARDIE
                     KDIARNKVILQK"
     gene            1348..1851
                     /gene="ribH"
                     /locus_tag="PLX53_04900"
     CDS             1348..1851
                     /gene="ribH"
                     /locus_tag="PLX53_04900"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOY72452.1"
                     /translation="MATKLKNLSAYDPAEIPSAKKMKFGIVVSDWNEEVTKALLEGAY
                     QTLIGNGVKEDNILVKHVPGAFELTLGAQFMAEYADLDGVICLGCVIQGETRHFDFIC
                     QSVTQGITQLNMDYNIPFIFGSLTTENLEQALERAGGKHGNKGVEAAITAIKMAHLQQ
                     EMEQLEG"
     gene            2249..2605
                     /locus_tag="PLX53_04905"
     CDS             2249..2605
                     /locus_tag="PLX53_04905"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HOY72453.1"
                     /translation="MEHINYTSENERTFAMLCHLSALAGCIIPLGHILGPLIVWLIKK
                     DEFPEVDRQGKAALNFQLSVTIYGLIAGVLVILLVGIVLLAILAVFTLVLVIVASVKT
                     ANAEKFEYPLAITFLR"
BASE COUNT          701 a          561 c          590 g          756 t
ORIGIN      
        1 taacaccatt catatactta cgtcgctcct cacctgactc gtctataagc gccgaatcgg
       61 tgggggagac cataacgatt ggaataaagc caatatgatc ggccagccgg tcgtaggttt
      121 tcccgtttcg tcggaaaatc ttaccttcat cgagtttgaa accgcagtaa atatcctcgt
      181 tgctcgacac cctttggtag cttccctcaa ttacaaaaaa ctgttctccg tgcctgagca
      241 cctgcctgtc gctcgagtta aaggcgcttt tgcacatcga aaggtagtaa atggcatcca
      301 acaggtttgt ttttccctgt ccgttgttgc caataaagca gttgatcttg gggttaagtt
      361 ccagctcaac ctgtctgaac gatttgaagt taatggccga tagatgttta aggtgcatgg
      421 caggtggaga cttttgctat gctacaaagg taattatttg taaaatgttg gcgcaaagtt
      481 ttcagatatt ccaaaatcca ttacttttgc gtggacttaa atcattacta acccgtttta
      541 acaatggcaa agaataacaa aaatgctcaa cccgacacgg ttgagtctat tgataacgct
      601 ttaagccaaa ctgaaaggtt tattgagcag aatcagaaga gtttaaccat cataatagtg
      661 gctatactcg tcatcatcag cctgtacttt gggtacaagc gcttttacct ggtacctaag
      721 gaggatgagg ctcgttcaca gatatttcag gctgaatact actttgagaa ggactcgttc
      781 aagctcgccc ttaacggcaa cgagaacgac ttgggatttg ttgatattgc cgataactac
      841 agcatgacca aaacgggaaa tctggcctgc tactacgccg gcatttgcta ccgtcagctt
      901 ggcgacttca acaaggccat cgagtacctt aaaaagtttg atgcgggtga tattatggtt
      961 acgccaattg cctatggcgc tattggcgac tgctatgtgg agcttaagca gcttgatgag
     1021 ggggtaaagt tctacataaa ggcagccgag tacagcgata acgatttcac ctcacccttt
     1081 ttctacaaaa aggcaggctt aatttatgag gtgatgaaca ataacggcga tgcccttaag
     1141 atttaccaga ccattaagga taagtatccc aagagcaacg aggcaagaga tattgagaag
     1201 gatattgccc gaaataaggt aatacttcaa aaatagccat gatgaggggt gctgcttgta
     1261 ggcacccctt tctatttatt atttcccttt atgttgactt gttggaaatc atagcttaaa
     1321 tttggtaaaa ttaatagctg cagtaacatg gcaacgaagc ttaaaaatct atccgcctac
     1381 gatcccgctg aaattccttc ggcaaaaaaa atgaaattcg gaattgtggt atccgactgg
     1441 aatgaggagg ttaccaaggc gctgcttgag ggtgcatacc aaacactcat tggtaatggc
     1501 gtcaaggagg acaacatact ggttaagcat gttccgggag cttttgagct gacccttggg
     1561 gcacagttca tggccgaata tgccgacctc gatggggtga tttgtctggg ttgcgttatt
     1621 cagggcgaaa cccgccactt cgatttcatc tgccagagcg ttacccaggg aattacccag
     1681 ctgaatatgg attataacat tcccttcatc ttcggctcgt taaccaccga aaatctcgaa
     1741 caggctttag agcgagccgg cggaaaacat ggcaacaagg gcgttgaggc tgcaatcacc
     1801 gcaattaaaa tggcacacct gcagcaggag atggagcagc tggaaggata agcacaacca
     1861 ccccggcggg atttcttgcc agtataggat ttgccatttt acttgcaaca aggttttacc
     1921 ttgttccaac cacccaaggt tttaagattt ttagttgact ataagctgcg atttggcatt
     1981 ctgcacttgt ttacgctccg atgaatttgg gagggctcac cgtaggggta ataatatttg
     2041 tttggacttg ccgaactagc aaataatgcc cggttattaa tccgattgtt tacaagcctt
     2101 actacatggg tattgcaggc taacttcatt ctcgcatggg gtaatctcca cctcttattt
     2161 tttgtttttt gctaaaattt ctttctatcc tgcagcaaga ttgcttaatt cgtagtctta
     2221 tatttgtaat ctaccaactg gaatttgtat ggagcatatc aactacacat cggagaatga
     2281 gcgaactttt gccatgcttt gccatctttc ggcgttggct ggctgtatca ttccattagg
     2341 gcacatcctt ggcccgctga tagtttggct tattaaaaag gatgaatttc ctgaagttga
     2401 taggcaggga aaggctgcgc ttaactttca actttctgta accatctatg ggctcattgc
     2461 aggggtgttg gttattctgc tggtgggcat tgttcttctg gcaattctgg ctgtgtttac
     2521 cctggtttta gttattgttg cttcggtaaa aacagccaat gctgaaaaat ttgaataccc
     2581 cttagccata acctttcttc gctagccc
//
LOCUS       DAOOQX010000248         2606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_47957_length_2606_cov_3.282634, whole genome shotgun sequence.
ACCESSION   DAOOQX010000248 DAOOQX010000000
VERSION     DAOOQX010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47957_length_2606_cov_3.282634"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PLX53_04910"
     CDS             <1..497
                     /locus_tag="PLX53_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72454.1"
                     /translation="PPQPTTEQFENLLFEQLLKLDPLKPIWIEDESSSIGTIFIPPTI
                     YSRMVKCPTIVVDVAKEERVARLVEDYARFSADDLVLSVTRMTKRMGGDRAKQCIDYI
                     RAGNFKEAIDIALSYYDKTYNYGLMSKTAKPITIKTSTKTENSLSLLLAAKNSIGELS
                     WPTD"
     gene            complement(487..1329)
                     /locus_tag="PLX53_04915"
     CDS             complement(487..1329)
                     /locus_tag="PLX53_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOY72455.1"
                     /translation="METKPQTFVIPWDFTPVAENAFKYAVKLSKVSNEPSQIDLVHVL
                     HSGGSPTKSGKTSEEQVQEMIQADKQRLSTTYSVEINTKLLEGSLFDVITDYASEVHA
                     NMVIMGTHGIKGVQKLTGSWALKVIAGSDVPFLVVQDEPAKDRIFSQLVMSIDFRDEE
                     KEKILWASRMAKQYGSKIHVIVPNSFDSGVKKKISNNLAFAKKQFENVGISWEQHNAA
                     KGKSFQEEIVNLAVDLEADLILIMTAPNLDFTDYVFGAQEQYIIANRSKIPVMCINFG
                     TINP"
     gene            complement(1480..2454)
                     /locus_tag="PLX53_04920"
     CDS             complement(1480..2454)
                     /locus_tag="PLX53_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOY72456.1"
                     /translation="MFTADQLSAIFSELLQGYSFEKEPRKLYEPIRYVLSLGGKRIRP
                     ILTLAACNMFSDDIEPAKIPALAIEVFHNFTLIHDDIMDNATIRRGKETVHEHWDTNV
                     AILSGDAMSILAYKLLTQTHLEYLQQVMNIFNSSALGVCEGQQLDMDFESREMISRDE
                     YIKMIELKTAVLLKCALQIGATLGNATTRQVELVGQLGTYLGLSFQLQDDLLDAFGEV
                     GAFGKAIGGDIVAGKNTILPVETTALISPEEAGKFIKILKANNLKESDKISMIMDYYN
                     RVDVKAVVEELIAQYQTKAAEIFEKLDIDNNRKTTMHTIMDSLVGRKK"
BASE COUNT          733 a          594 c          503 g          776 t
ORIGIN      
        1 aaccacccca gcccaccact gagcaatttg agaatttact tttcgagcaa ctcctaaagc
       61 tagatccctt gaaacctata tggattgaag acgaaagcag cagcataggt accattttta
      121 tccccccgac catttactcc cgaatggtaa aatgcccaac aattgttgtt gatgtggcta
      181 aagaagaacg ggttgcccgg ctggttgaag attatgcccg attttcagca gatgacctgg
      241 tgctttcggt aaccagaatg acaaaaagaa tgggtggcga cagggctaaa caatgcattg
      301 actacatcag ggcgggtaac tttaaagagg caatagacat tgccctatca tactatgata
      361 aaacctataa ttatgggtta atgagcaaaa ctgccaagcc tataaccatt aaaacttcga
      421 ccaaaaccga aaacagctta agcctcctgc ttgctgctaa aaattccatt ggggagttga
      481 gctggcctac ggattaatag tcccaaagtt aatgcacatt acaggaatct ttgatcggtt
      541 ggctatgatg tattgctcct gagccccaaa cacgtaatcg gtaaaatcca gattaggagc
      601 tgtcattatt agaattaaat ctgcttcaag gtcaactgca aggttcacaa tttcctcctg
      661 aaaacttttc cccttggcag cattatgctg ctcccagcta atgcctacat tttcaaattg
      721 tttcttggca aaagcaaggt tattgctaat cttcttttta actcccgagt cgaagctgtt
      781 ggggacaata acatgaattt tagagccata ctgtttggcc atcctcgaag cccataagat
      841 tttttccttc tcctcgtccc tgaaatcaat tgacatcacc agctggctga aaatcctatc
      901 cttagcgggt tcgtcctgaa caaccagaaa aggaacatct gaaccggcta ttacctttag
      961 cgcccagctg ccggttagtt tttgcacacc cttaatacca tgggtcccca taatgaccat
     1021 gttggcatga acctcgctgg cgtaatccgt aattacatcg aagagacttc cttccagtag
     1081 ctttgtgtta atttcaaccg aataggtggt gcttaaccgt tgtttatctg cctgaatcat
     1141 ttcttgaacc tgctcctcag atgtcttgcc cgacttggtt ggagagcctc ctgaatgcag
     1201 cacatgcaca aggtctatct ggctgggttc gttgctcact ttcgagagct ttacagcata
     1261 cttaaaagca ttctcggcaa caggtgtaaa gtcccatgga ataacgaatg tttgaggctt
     1321 agtttccatt tcgctaaagg tttagtataa tctttgttag caaatattta tgataaattt
     1381 atggcatctt tcagtaataa caaaatttat tgtaataagc ttactgcaaa aaattattgg
     1441 aataaaataa ccaataaaag cagcacaccc tttgcacaat tatttttttc gtcctaccag
     1501 gctatccata atggtatgca tggttgtttt tcggttgttg tctatgtcaa gtttttcaaa
     1561 gatttcagct gcttttgttt ggtactgggc aatcaactcc tcaacaacag ctttgacatc
     1621 aacccggttg tagtaatcca taatcatcga tattttatcg gattctttca agttgttggc
     1681 ttttagtatt ttaataaatt tacccgcttc ttcgggcgaa atcagcgcag tagtttctac
     1741 aggtaggatg gtgtttttac cggctacaat atcgcctccg attgccttac caaaagcacc
     1801 tacctcaccg aatgcgtcga gcaaatcgtc ctgaagctga aaggatagcc ccaagtaagt
     1861 gccaagctgt ccaacgagtt ccacttgacg ggtagtggca tttcccaggg ttgctccaat
     1921 ttgaagagcg cactttaaca gcactgcggt cttcaactct atcattttta tgtattcatc
     1981 tctggaaatc atctccctgc tttcaaaatc catgtcgagc tgctgcccct cgcaaacccc
     2041 caaagcactc gagttgaaaa tgttcatcac ttgctgaagg tattctaaat gggtttgggt
     2101 aagcagcttg taggccagaa tagacattgc atctcctgaa agaatggcaa cattggtatc
     2161 ccagtgctcg tggacagttt cttttcccct tcttatggtg gcgttatcca ttatatcgtc
     2221 gtgaatcaag gtgaagttat gaaaaacctc tatggctagt gcaggaattt tggcaggctc
     2281 aatatcgtcc gagaacatat tacatgctgc tagcgtaagt ataggcctga ttcgtttccc
     2341 tcccaaactt aaaacatagc gtataggttc gtagagttta cggggttctt tttcgaagga
     2401 gtaaccttgc agtaactctg aaaaaattgc actcagctga tctgcggtga acataaccaa
     2461 ataaaagggt tagaataaat ttaacttcct gaaaatccta gccaacaact atacaaccaa
     2521 tgattttaac cagcactaaa gcatctctct tagccttttt atatttttcc aacgcagacc
     2581 ccacatccgc ttttgcattt ttccaa
//
LOCUS       DAOOQX010000249         2601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_48096_length_2601_cov_3.697565, whole genome shotgun sequence.
ACCESSION   DAOOQX010000249 DAOOQX010000000
VERSION     DAOOQX010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48096_length_2601_cov_3.697565"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLX53_04925"
     CDS             <1..732
                     /locus_tag="PLX53_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY72457.1"
                     /translation="ASAFMPMIRLHFNQPDKPATNNNLASVYLDSINATLPINYKLIF
                     GFAPSNSTEKLSIESADSTIANFPALCLVKGIFLAENGKFASATNEFKKAFEQNSSNP
                     IYLFSYIMNNVEMAEFIENFNTSTEISGIAMSSSKNTGITSITAYNDAIVELKGLYEV
                     FPKNGLIAYNLGNLSIMTNDYESAISWFSLAVRENPLLSCAWFNKGLSLLITGNKDEG
                     CNNLGKAGELGVEEAYMAIKKFCTR"
     gene            966..1685
                     /locus_tag="PLX53_04930"
     CDS             966..1685
                     /locus_tag="PLX53_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOY72458.1"
                     /translation="MGKLFDRLNSDVRFIEGINGCINCGTCTAICPAAEVYDYEPRSI
                     ANLVQTHDDEVIENLLKSDTIWYCGECMSCKTRCPRNNAPGLIIQALRKLSIETGLFV
                     ESEKGRQQLSLKRMLGNSILETGYCVHFDHTELSMFPELGPVWQWVRDNRETVLKKVG
                     ANYKGTGPGAIRKISQESLDELERIFEVTGGLEWFKKIEEDSSEMADKLSLEIGNETS
                     KESPYFRKVYRHNDPEKHTRK"
     gene            1798..>2601
                     /locus_tag="PLX53_04935"
     CDS             1798..>2601
                     /locus_tag="PLX53_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOY72459.1"
                     /translation="MKGNEVKKKIWSEYQKEIADDKFYFVRSCIRQTFFPGSDTVYLK
                     ILRDVLGKDVYEDANHTTCTGIGYHCEVVPFPTIQTVVARNFSLMRENGYQNYNPSCV
                     TSFGIYTEILETWHHFPEEKEKTREYLKKATGREFEEPANLVHTSDVLYKFRNEIAAK
                     AKYRLVNRETGKPLKVVDHIGCHYAKMFPSKGIGGAEYPQVLAGLVEAFGGEVVDYPE
                     RRHCCGFGFRQYFVQANRGYSLAASKKKFESMEPFKPDFIITNCPGCNYF"
BASE COUNT          842 a          557 c          566 g          636 t
ORIGIN      
        1 gcctctgcat tcatgccaat gataaggctt cattttaatc aacccgataa accagcaaca
       61 aataacaatc ttgcatccgt ttaccttgat tctattaatg caacacttcc cataaactac
      121 aagttaatct ttgggtttgc tcccagcaac tcaacagaaa agctttccat agaaagcgcc
      181 gattccacta tagccaactt tccggcatta tgtctggtaa agggtatatt tttagcagaa
      241 aatggcaagt ttgctagcgc cactaacgaa tttaaaaagg cctttgagca aaatagcagt
      301 aacccgattt acctcttctc ctacatcatg aataatgttg aaatggcaga attcattgaa
      361 aattttaaca cctcaacaga aatctccggc attgctatga gctcttcaaa aaatacagga
      421 ataacaagca ttacagcata taatgatgct atagtcgaac taaaaggttt atatgaagtg
      481 ttccctaaaa atggtcttat tgcttacaac ctggggaatc taagtattat gacgaacgac
      541 tatgaaagcg ccattagctg gttctcgctg gcagtaagag aaaatccact tctgagctgt
      601 gcatggttta acaaagggct tagcctactt attaccggca acaaggatga gggctgcaat
      661 aacttaggta aagccggaga gctaggtgtt gaggaggcct acatggcaat caaaaaattt
      721 tgcaccaggt aaacagcttt atataaaatt gaaagccaag cccgaattag cggggtgccg
      781 gctaaagcca ctctcaaaaa agttagctgc tcttctctag gagcattgac attaattacc
      841 aactacaata aaaattgata aatattagct ggtaaataat atcatggtaa aatttttaca
      901 tttgctgtta caataataac attaatcttg atagatcatt ttggctatca ggcaaatagt
      961 ataacatggg aaaactattt gacaggttga acagcgatgt caggtttatt gagggaatca
     1021 acggctgcat aaactgtgga acctgcactg caatctgccc ggcggcagaa gtttacgact
     1081 atgaaccccg aagtattgcc aatctggttc aaacccacga cgacgaagtg attgagaact
     1141 tgctgaaaag cgatacaata tggtactgcg gggagtgcat gagctgcaaa accaggtgcc
     1201 cccgaaataa tgccccaggg ctaataattc aggcactccg aaagctctcc atagaaacag
     1261 ggctatttgt agaatccgaa aaagggaggc agcagctctc gcttaaacga atgcttggca
     1321 actccattct cgaaacaggc tactgcgtcc actttgacca taccgagcta agcatgtttc
     1381 ctgagctagg ccctgtttgg caatgggtta gggacaaccg cgaaaccgtg ttaaaaaaag
     1441 taggcgccaa ctataaaggc acaggccccg gggcaataag gaaaatttca caggaaagtc
     1501 tcgatgagct ggaaaggatt tttgaggtaa ccggagggtt ggaatggttt aagaaaatag
     1561 aggaggattc ctcggaaatg gccgataaac ttagtctcga aatcggcaac gagactagca
     1621 aggagtcacc atacttccga aaggtttaca gacacaacga tcctgagaag cacacacgta
     1681 agtagtttaa ataaagctag ctgcctgcac aactatacgg tattttaaaa aatgggagca
     1741 atcctcctac cattttcaca aacgtaaaga atatctgagc taaacataac aggaaccatg
     1801 aaaggcaacg aggtaaaaaa gaaaatttgg agcgaatacc agaaggaaat tgcagatgat
     1861 aagttttact tcgtccgcag ctgcatacgt caaacatttt tcccgggcag cgatacagtt
     1921 tacctcaaaa tactgcggga tgtgctgggt aaggacgttt acgaggatgc aaaccacacc
     1981 acctgcaccg gaattggcta ccactgcgag gtggttcctt ttccaaccat ccaaacagtt
     2041 gtggcgcgca acttttccct gatgcgtgag aatggttacc aaaactacaa cccatcctgt
     2101 gtaacctcat ttggtatata caccgaaata cttgaaacat ggcaccattt ccccgaggaa
     2161 aaggaaaaga ctcgtgaata ccttaaaaag gcaaccggaa gggaatttga agaacccgcc
     2221 aaccttgtcc atacaagcga tgtgctctat aagtttcgta acgaaatagc tgcaaaagct
     2281 aaatacagac tggtgaatag ggaaaccgga aaaccgctga aggtagttga ccacattggc
     2341 tgccattatg ccaaaatgtt tcccagcaaa gggattggcg gtgccgaata cccccaggtg
     2401 ctggcaggtc tggtagaagc ttttggaggc gaagtagttg actatcctga gcgtaggcac
     2461 tgctgtgggt ttggcttccg ccagtacttt gttcaggcca acaggggata ctccctggct
     2521 gcttcaaaaa agaaatttga atccatggaa cccttcaagc ccgatttcat cattactaat
     2581 tgtccgggtt gtaactattt t
//
LOCUS       DAOOQX010000250         2593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_48302_length_2593_cov_2.662333, whole genome shotgun sequence.
ACCESSION   DAOOQX010000250 DAOOQX010000000
VERSION     DAOOQX010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48302_length_2593_cov_2.662333"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLX53_04940"
     CDS             complement(<1..315)
                     /locus_tag="PLX53_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOY72460.1"
                     /translation="MKRSIEAILEQYDHAGMDSLIPILQEIQNKTGCLNEESIVMVGK
                     HLGIPTSKVYGLATFYNQFKFEPKGKFHILVCNGTSCRLNGSKWIIDFIEKNLKIKNG
                     ETS"
     gene            596..847
                     /locus_tag="PLX53_04945"
     CDS             596..847
                     /locus_tag="PLX53_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOY72461.1"
                     /translation="MEKKNEIVICLGSSCFARGNKATLKTIQKYIDDHHLREKVYFHG
                     GHCFGRCAEGPTVTVNGKIFTGVDEYNILDILNSELSEY"
     gene            866..>2593
                     /locus_tag="PLX53_04950"
     CDS             866..>2593
                     /locus_tag="PLX53_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOY72462.1"
                     /translation="MSQLFEISEETCINCYACVRVCPVKAIEVKAGQDFARILQNRCV
                     GCGSCLNICPVNAVSYRSSKKNVLELLKGNEPVAAICSPSISGEFVDITDYRKFVQMI
                     KTLGFTYVCEVTFGVDLVAREYKKLFDDFKGKYFITSMCPVVVSMVEKYHPEMVNNLA
                     PIISPIIATAQAVHNQYGSNVRIVHIGPCIQSKEEAKLYNGKSQIDEVLTFVELRQLF
                     EEFNINESNLEYSEFDEPIGYKGSLYPIGRGILQAVDISEDLLTGTVISANGPNNTLQ
                     ALEQFETSAEMINRHFNLFYCEGCLMGPGTSKGGKKFIRRTMVVNYANKRLLTFNRKK
                     WEENISRNEKNSYTRDFTPDDQRLPLPSQQKLEKVLKVIDKEFVGKETGCEACGYASC
                     YDFASAVASGLAHTDMCLNFTLKNRQEYIKSLRSTNEKLAKTQEALRESEKKARIEQE
                     AAREASETVTTMLKKLPAAVVLIDKKLKIIQSNQSFIDLLGDDALEINEIVPGLVGAD
                     LKSLVPYNIHNLFTYVLQNNSDILNRDVHYRDKLLNISVFTIRPNSIVGAVIRDMYAP
                     EVRKEEVIKR"
BASE COUNT          811 a          534 c          573 g          675 t
ORIGIN      
        1 actagtctct ccgtttttaa tttttaaatt cttctcaatg aaatcaatga tccattttga
       61 gccgttcagc ctgcacgaag tgccattgca taccaatata tggaacttac cctttggctc
      121 aaatttaaac tggttgtaaa atgtagccaa accataaact ttggaggtgg gtatgcccaa
      181 atgcttacca accatgacaa ttgactcctc atttaagcat ccggttttat tctgaatttc
      241 ctgcaaaatg gggattaagc tgtccattcc ggcatggtca tactgttcaa gtatagcttc
      301 aatgcttctt ttcattaccc atcaagaaat ttttaaaaaa ttgttaactc aataacaatt
      361 aactattttc ctgtataaca gatatttaag ttggtatttt cacctgttaa ttcccgaaca
      421 aattaataaa agaaatttag ataatctaac tttatttaaa tttgtgattg aattaacaga
      481 agttttttta agtaagccag caagagggtt aatcattaaa cgctcagagg aaacaattta
      541 aaggattggc aggagaacat gggaaaggta ttataaagcc cagccagaca ataagatgga
      601 gaaaaaaaac gaaatcgtga tatgcttggg tagctcctgt tttgcgaggg gaaataaagc
      661 aaccctaaag accatacaga aatacataga cgatcatcac ctaagggaaa aggtttactt
      721 tcacgggggg cattgctttg gcagatgcgc tgaagggcct acagtaaccg ttaatgggaa
      781 aatttttaca ggcgttgacg agtacaatat tctggatatc ctaaatagcg agttgagcga
      841 gtactaatcc aaacgattaa aacaaatgtc gcagctcttc gaaataagtg aggaaacgtg
      901 cataaactgt tatgcttgtg tgcgggtttg cccggtaaag gccatcgaag ttaaagccgg
      961 tcaggacttt gcccgaatcc tccaaaacag gtgtgtgggt tgtggaagct gcctcaatat
     1021 ttgcccggtg aatgcggtaa gctaccgttc ctccaagaaa aacgttctgg agctacttaa
     1081 gggcaacgaa ccggttgctg ctatttgctc gccaagcatt tcgggtgaat ttgtggatat
     1141 aactgactac cgaaagtttg ttcagatgat aaaaacctta gggtttactt acgtttgcga
     1201 agttactttt ggtgttgacc ttgtagccag ggagtataaa aagctgttcg atgactttaa
     1261 gggaaagtac ttcatcacca gcatgtgccc ggtggttgta tcaatggttg aaaagtacca
     1321 tcccgaaatg gtcaacaacc ttgcccctat catttcgcct atcattgcta ccgcccaggc
     1381 tgtacacaac cagtatggaa gtaatgtccg gattgttcat ataggcccct gcatacaaag
     1441 caaggaggaa gcaaagctat acaacgggaa aagccagatt gacgaggtgc ttacctttgt
     1501 ggagctccgc cagctattcg aggaatttaa cataaacgag agcaacctgg agtactccga
     1561 gtttgacgag ccaattggct acaagggatc gctttacccc attggcagag gcatactgca
     1621 agccgttgac atcagcgaag acttgctaac cggaacagtt atcagcgcta acgggcccaa
     1681 caacacactt caagccctgg agcaattcga aaccagcgcc gaaatgatta accggcattt
     1741 taaccttttc tactgcgagg gctgccttat gggaccgggt acatcgaagg gtggaaaaaa
     1801 attcattcgc cgaacaatgg tggttaacta tgccaacaaa agattgctaa cgtttaaccg
     1861 taaaaaatgg gaggaaaaca tatcccgaaa tgaaaagaat agctatacaa gggatttcac
     1921 ccctgatgac caaaggctgc cactcccctc acagcaaaaa cttgagaagg tgctaaaggt
     1981 aattgacaag gaatttgttg gaaaggaaac aggttgtgaa gcttgtggat atgcctcatg
     2041 ctacgacttt gcctctgcag tggcatcggg gcttgcccat acggacatgt gcttgaactt
     2101 caccctaaaa aacaggcagg agtatattaa atcccttcgt tcaactaacg agaagcttgc
     2161 taaaacccag gaggctttga gggaatcgga aaaaaaggcg cgaatagagc aggaagcagc
     2221 tcgtgaggct tccgaaacgg ttaccaccat gcttaagaag ctacctgctg cagttgtgct
     2281 tattgataag aagctaaaaa tcatacagag caaccaaagc tttattgatt tgcttggcga
     2341 tgacgcccta gaaataaacg agattgttcc cggacttgtt ggtgcagacc taaaaagcct
     2401 ggtaccctac aatattcaca acctgtttac ctacgtactt caaaataata gcgatatatt
     2461 aaaccgtgat gttcactacc gcgataagct gctgaacatc tccgtattca ccatcaggcc
     2521 caacagcatt gttggtgctg ttatcaggga tatgtatgct ccggaggtaa gaaaggaaga
     2581 ggtgattaaa cgt
//
LOCUS       DAOOQX010000251         2591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_48348_length_2591_cov_3.300473, whole genome shotgun sequence.
ACCESSION   DAOOQX010000251 DAOOQX010000000
VERSION     DAOOQX010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48348_length_2591_cov_3.300473"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..852)
                     /gene="nadD"
                     /locus_tag="PLX53_04955"
     CDS             complement(244..852)
                     /gene="nadD"
                     /locus_tag="PLX53_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOY72463.1"
                     /translation="MKIGLFFGSYNPIHIGHLVIADFILEFSDLDEIWFVVSPQNPFK
                     SIDELAADEHRLNMTRLAIPEKNTRMKVCDVEMGMPRPSYTIDTLRRLEQESPGCTFV
                     ILLGSDNIAGIHDWKESAALINGWDFYVYPREGSFNPKNLPSGRFTLIDAPVIGISST
                     QIRHLIAHGVDASGYVPDDVWDYIIKNKIYGYGTTLTANADK"
     gene            complement(849..1424)
                     /gene="gmk"
                     /locus_tag="PLX53_04960"
     CDS             complement(849..1424)
                     /gene="gmk"
                     /locus_tag="PLX53_04960"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOY72464.1"
                     /translation="MDGKLLIFSAPSGSGKTTIVKHLLDCFPELEFSVSACSRPPRKG
                     EINGVDYHFLTVEEFKKRIAGNEFVEWEEVYPNLFYGTLWSEIHRIWNKGNHVVFDVD
                     VKGGLNLKKKFPDCSLAVFVMPPSMEDLKQRLIKRGSEDDESLALRLNKAKEEIGYAS
                     QFDVVLINNQVEKAFMEAERIVRDFIKIDQT"
     gene            complement(1476..2357)
                     /locus_tag="PLX53_04965"
     CDS             complement(1476..2357)
                     /locus_tag="PLX53_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOY72465.1"
                     /translation="MLVSMTGFGRAEMQLGSKKLIAEIRSLNSKQLDLSIRIPSIYRG
                     IEMDIRSIIAQQLVRGKVDFQLSFEETAESATTPINRDIFEGYLLQIKEIARQFEIDI
                     TNESLVQTILRLPDVLRVNGKDVVGEDELNSVKKLCSNAVESLVNFRRQEGSVLYNDI
                     KNRVLLILDLLQKVEPYEKQRIEDVKKRLREALKTLEDDVKINSDRFEQEVIYYLEKL
                     DITEEKVRLKNHCKYFLETAGLDEQVGRKLGFIAQEMGREINTMGSKANHSDIQKVVV
                     QMKDELEKIKEQSLNIL"
BASE COUNT          728 a          630 c          496 g          737 t
ORIGIN      
        1 cccgaacgcc atcacaacct aattccggtg aataaggagg ctatcctgaa aggaatggaa
       61 ctagcaaaaa aatacagcta gctaaagttg aatgttaatg gtattctcca ctaaaagccc
      121 cagcatttgg ggcttttttc taactcaaat aacgcatatc tcaatttcga actagcattt
      181 acttcaccta ccgaacgcag ctatcaatcc taatgctcat aaggtgaatt agcaaaccaa
      241 aatttatttg tcagcattag cagtgagcgt tgtgccataa ccgtaaatct tgttttttat
      301 gatgtaatcc cacacatcat cgggaacgta tccggaagca tcgacaccat gggcaatcag
      361 gtggcgaatt tgagttgacg aaatccctat tacaggagcg tcaataaggg taaagcgccc
      421 ggaaggtaaa tttttaggat taaaggaacc ttcacgggga tagacataaa aatcccagcc
      481 atttatcagg gcggcggatt ccttccaatc atggatacca gcaatgttat ccgaacctaa
      541 gagaatcaca aaagtgcacc cggggctttc ctgctccagt ctcctgagcg tgtcaattgt
      601 gtaggagggt cggggcattc ccatttcaac atcgcacacc ttcatccggg tgttcttttc
      661 gggaatagcc agccttgtca tgttaaggcg atgctcatcg gcagcaagct catcaatgct
      721 tttaaaagga ttttgagggc ttacaacaaa ccaaatctca tcaagatcgg aaaattcaag
      781 aataaaatcg gctataacca gatggccgat gtggatgggg ttgtaggaac caaaaaataa
      841 gcctattttc atgtctggtc aatttttatg aaatccctga caattctttc agcctccata
      901 aaagccttct ctacctgatt gttaattaaa acaacatcaa actggctggc atacccaatt
      961 tcctcctttg ctttgtttag ccttagtgct agcgattcat cgtcttcgga gcctcgtttt
     1021 ataagcctct gcttaagatc ctccatagag gggggcatga caaacacagc cagggagcaa
     1081 tcgggaaatt tctttttcag gttcaatccg cccttaacat ccacatcaaa caccacatga
     1141 tttcccttgt tccatattct atgaatttca gaccataagg ttccgtagaa caagttcgga
     1201 tacacctcct cccattcaac aaactcgttg ccagcaatac gcttcttaaa ttcctccacc
     1261 gtgaggaaat ggtaatctac tccgttaatc tcacctttcc tgggaggccg gctgcatgcc
     1321 gaaaccgaaa attctaactc gggaaagcaa tcgagcaggt gcttaacaat ggttgttttg
     1381 cccgatcccg aaggtgctga gaatataagt agcttgccat ccatgccgtt aagtgaggag
     1441 taatagtagc aaagtttaga agaatttaca aacggttaaa gaatattgag cgattgctcc
     1501 ttgattttct ccagctcatc cttcatttgc accacaactt tctgaatatc tgaatggttg
     1561 gccttagaac ccatggtgtt gatttccctc cccatttcct gcgcaataaa gcccagcttc
     1621 cttccaacct gctcatccaa ccccgctgtt tccaggaaat acttgcaatg gtttttcaac
     1681 cttacctttt cctccgtaat atcgagcttt tcaaggtaat agataacctc ctgctcaaac
     1741 cgatcggaat taatctttac atcatcttcg agcgttttta acgcctcccg aagccttttc
     1801 ttcacatcct cgattcgctg cttttcgtag ggttcaacct tttgaagcaa atcaagaatg
     1861 agcaaaaccc tgtttttaat atcgttatac agcacgctgc cctcctgcct gcggaagttt
     1921 acaagactct ctaccgcatt ggagcaaagt tttttaactg aattgagctc atcctcaccc
     1981 acaacatcct tgccgttaac acgaagcaca tcgggtaacc ttagaattgt ctgcactagc
     2041 gattcgtttg taatgtcaat ttcgaactga cgggctattt ctttaatctg cagcaggtag
     2101 ccttcaaaaa tatccctgtt aattggggta gttgccgatt cggctgtttc ctcaaaactc
     2161 agctgaaagt caacctttcc cctgaccagc tgctgcgcaa taatggaacg gatatccatc
     2221 tcaatgcccc tgtagatgga tggaatcctg atgcttaaat cgagctgctt gctgttgagc
     2281 gagcgaattt ccgctataag ttttttactg cccagctgca tttccgccct tccaaaaccc
     2341 gtcatcgaaa ctagcataat tcctgtttta atttgtacaa aggtaattta gaattattca
     2401 ttatagccaa ccagcgcacg aaatcgtcat tcaagctgaa attcatgttt ttaacggacg
     2461 gtgctcctct ccaaacactt ttacctggtt tcgatgtaaa ctacagtaag gcataattac
     2521 cggtggaatg ctatctttgc acccgcaaac aatgtaaatg taaggtatat ggcaaacaga
     2581 gggttgatgg c
//
LOCUS       DAOOQX010000252         2581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_48589_length_2581_cov_2.844418, whole genome shotgun sequence.
ACCESSION   DAOOQX010000252 DAOOQX010000000
VERSION     DAOOQX010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48589_length_2581_cov_2.844418"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1130)
                     /locus_tag="PLX53_04970"
     CDS             complement(180..1130)
                     /locus_tag="PLX53_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329363.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOY72466.1"
                     /translation="MSDVYPWGDNRRFNTYSGYFKREFGGRVQKLAIDAGFSCPNRDG
                     TIATGGCTYCNNEAFNPSYCHPTKSITQQIEEGIEFHKVRYRRAKRFLAYFQAYSNTH
                     APIEHLRMLYEEALAFPGVIGLVIGTRPDCVDEEKLNYIGQLAAKHYVTLEYGVESCY
                     NRTLEAINRGHTFEQSVWAIEETHKRGIKVGAHIILGLPGETRNEMLAEASILSSLPI
                     HTVKFHQLQVIMGTVMAKRYELDPGAFQLFGMEEYLNFMVDVVERLNPNMVVERITGE
                     APPRFLATNGWGSYRTDQLMVMFEKLLEQRNTWQGRLYKG"
     gene            1576..>2581
                     /locus_tag="PLX53_04975"
     CDS             1576..>2581
                     /locus_tag="PLX53_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOY72467.1"
                     /translation="MAKEATELEKPTPVAGENVNTVNLENFDWDLYAREGAYTPEQRK
                     ELEDIYDKTLSTVAENEVVEGTVVAITKREVFVNVGYKSEGVLSISEFRYNPDLKVGD
                     KIEVYVESSEDKRGQLVLSHKNARVLRSWDRVNAALEKDEIIKGYIKCRTKGGMIVDV
                     FGIEAFLPGSQIDVKPIRDYDIYVGKTMEFKVVKINQEYKNVVVSHKALIEAELEQQK
                     KEIISKLEKGQVLEGTVKNITSYGVFIDLGGVDGLIHITDLSWGRVNHPEEVVQLDQK
                     LNVVILDFDDAKKRIALGLKQLTPHPWDSLDTNLKVGDVVKGKVVVIADYGAFVEIDP
                     G"
BASE COUNT          684 a          543 c          599 g          755 t
ORIGIN      
        1 ctccatacaa ggggctgcgc cccttgctac ttattacgcc ccttcggggc ttaaatccat
       61 caggtcacct accagttccg ccggtaggcg aacaagtttt tgaattttta atccgccggt
      121 aggcggacaa gtttttgaat ttttaatttt gaatcttgaa tttttttatc ctttaccctt
      181 tatcctttat acaaccttcc ctgccaggta ttcctctgtt cgagtagctt ctcgaacatg
      241 accataagct gatcggttct gtagctaccc caaccattag tggccaggaa tcggggcgga
      301 gcttctcccg ttatccgctc caccaccatg ttggggttta acctttctac cacgtcaacc
      361 ataaagttga ggtactcctc catcccaaaa agttggaatg ccccaggatc taattcatac
      421 cgttttgcca tgactgtccc catgattacc tgaagttgat ggaacttcac ggtgtgaata
      481 ggaagggagg atagaatgct ggcttcagcc agcatttcgt tgcgcgtctc gcctggtagc
      541 ccaagaatta tatgagcacc aacctttatt ccccgcttat gcgtttcctc gatagcccaa
      601 accgattgct cgaaggtatg cccccggtta atcgcctcaa gcgttcggtt gtagcacgat
      661 tccacaccgt actcaagagt tacgtaatgc ttagctgcaa gctgtccgat gtagttgagt
      721 ttttcttcat caacacaatc ggggcgagtt ccaataacca gccctataac cccgggaaag
      781 gccagcgctt cttcgtaaag catgcgaagg tgctctattg gtgcatgggt attggagtag
      841 gcttgaaagt atgccagaaa tctttttgcc cgacgatagc gaaccttatg aaactcaatc
      901 ccttcctcaa tttgctgggt aattgatttt gtggggtggc agtagctggg gttaaacgcc
      961 tcattattac agtaggtgca tccaccggtt gcaattgtac cgtcacggtt cggacacgaa
     1021 aaaccggcat caatggccaa cttttgaact ctaccaccaa actcccgttt aaagtaaccc
     1081 gagtaggtgt tgaatcggcg gttatcgccc catgggtaaa cgtcgctcat ctacgtgcac
     1141 gcttgttttt ttggtgtaag tgtactggta gagcctttct gatattggcc tatagcaaaa
     1201 tcagctatct cagatggctg tttatgatgt gcaaaggtag cgatttcaat gaactgtata
     1261 ttcgtaaaag ttggtattgc atttttgctt gggtagctat aacttttact tcttgatctt
     1321 gttgagcact tcaaaattct gatttgtggt tctttgttgg caatatctgg ctacctatgc
     1381 aggtaatttg ttttttagct gttaaaataa ttttttatct ttatgaattt cagctatatt
     1441 gcagatactt tttagaaaaa aatggtattt tttcctataa atgttaaagt tatatatttt
     1501 tgcagtttga ttaattccca tacataatgt ctaactaatt aattgtaaat cgtttaaatg
     1561 acaaatcagg aagaaatggc caaagaggct acagaattag aaaaacctac ccctgtggca
     1621 ggagagaatg ttaacactgt gaatttagaa aattttgact gggatttgta cgccagggaa
     1681 ggggcctaca cccccgagca acgaaaggag ctggaggata tttatgacaa aactctatca
     1741 acagttgctg aaaatgaggt agttgaggga acagtcgtag ctattaccaa gcgtgaggta
     1801 tttgttaatg tcggctacaa gtcggaggga gtgctgagta tcagcgagtt tcgttataat
     1861 cccgatttaa aggtaggcga taagattgaa gtttatgttg aaagttcaga ggacaaacgc
     1921 ggacagctgg ttctttcgca taaaaatgcc cgtgttctac gctcatggga tcgtgttaat
     1981 gcagccctgg agaaggatga gataattaag ggatatataa aatgcagaac taagggcggt
     2041 atgatagtgg atgttttcgg tattgaggca ttcctgcccg gctctcaaat tgatgtgaag
     2101 ccaatacgcg actacgacat ctacgtggga aaaaccatgg agttcaaggt tgttaaaatc
     2161 aaccaggagt ataaaaatgt tgtggtgtcg cataaggcgc ttatcgaggc cgaactcgaa
     2221 cagcaaaaga aggaaatcat ctccaaactc gaaaaaggtc aggtgcttga gggtaccgtt
     2281 aaaaatatta ccagctacgg cgtattcatt gatttgggag gcgttgatgg gctcatccat
     2341 attaccgacc tttcctgggg ccgggtcaac caccccgagg aggttgttca gctcgaccaa
     2401 aaacttaacg tggtaatcct cgattttgat gatgccaaaa agcgtattgc ccttgggctg
     2461 aagcagctta caccccatcc ttgggattcg ctcgatacta acctcaaggt tggcgatgtg
     2521 gttaaaggta aggttgtggt tattgccgat tacggggctt ttgttgaaat tgaccccggc
     2581 g
//
LOCUS       DAOOQX010000253         2579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_48641_length_2579_cov_3.028130, whole genome shotgun sequence.
ACCESSION   DAOOQX010000253 DAOOQX010000000
VERSION     DAOOQX010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48641_length_2579_cov_3.028130"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PLX53_04980"
     CDS             <1..1048
                     /locus_tag="PLX53_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72468.1"
                     /translation="AEDNGEVTVKLQSINIDGFSTSQTETLHAARPILGDILYLVTSS
                     DTIELAKSPENPFLYLSPTGSYPNEVVGKIATSENLEAAEWVWVSSGSAIVTGSYTDS
                     DIRLSDPSYQVEQLSFNVMTFELKFIGELLPSVKFNTTTLLMGEDGRFHGEVEFTKGD
                     VVTVEGISNLAAAYNPDFFDYKDGVLTFKAPTGKYQVVYWAGTINYMWLRQDNAVYPD
                     ALWLTGAGIGMPTFINDVTNIWWGWDDWGVTTRQSYFFCPKIGENKYQVTVYLMNTEG
                     SWWLQTEFKLFPTKSWDNEFSSMDWGYISSNLSPNTNGNFVHTPELTEGLYRITIDMS
                     GGKGNYFLIAQKIN"
     gene            1218..>2579
                     /locus_tag="PLX53_04985"
     CDS             1218..>2579
                     /locus_tag="PLX53_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan endo-1,6-beta-glucosidase"
                     /protein_id="HOY72469.1"
                     /translation="MKNIHNSLFSFASGMLSACLIFFAILLPGCTDDSDSVDNPPDDT
                     TQLGDVRVWVTTCNQAYLFKEQSVSFSDDVAPFTIRVDTLNRYQQVEGFGAALTGSSA
                     YNLRRMTDSQRTSLLTELFDPVKGIGLSYLRLSIGSSDFSLSLYTYCDYPSIDSFALH
                     PTDLRDLIPVLKEILAINPAIKIMATPWTPPIWMKTSAAWSGGSLIPDFYDEYAEYFV
                     RYIRAMAGEGITIDAISVQNEPMWEFGTPGMKMTWQEQLTFIRDFLGPKFAAEDISTK
                     ILIWDHNWDMPDYPISILSDPAAYMYIAGSAFHGYGGEYSAMLSVHNQFPQKGLYFTE
                     ISGGGWATDFDDNLKWTIENVFFGTLSCFSKNVLLWNLVLDDNWGPFIQGGCSNCRGV
                     ITLNSDGSLTRFNEYYALAHFAKAIRPNAYMLGTTVVGDMSQLADFKYVAAQNPDGSK
                     VVVA"
BASE COUNT          616 a          619 c          609 g          735 t
ORIGIN      
        1 tgccgaggat aatggtgagg ttaccgtgaa gctccagtct atcaacattg atggcttttc
       61 tacttctcaa accgaaacgc tccatgcagc ccgacctata cttggcgata tcctttacct
      121 ggtaacttcg agcgatacca ttgagctggc aaaaagccct gaaaacccgt tcctctacct
      181 tagccctacg gggtcgtacc ccaacgaggt tgttggtaaa attgcaacct ccgaaaacct
      241 tgaagctgcc gagtgggttt gggtttcctc gggtagtgca attgtaaccg gcagctacac
      301 cgatagcgat atcaggctta gcgatccatc ctaccaggtt gagcagcttt ccttcaacgt
      361 catgactttt gagctgaagt ttattggcga gctgctgccc agcgttaagt ttaacaccac
      421 cacgctgctt atgggtgagg atggacgttt ccatggcgag gtagaattta ccaagggtga
      481 tgtggttacc gtagagggaa tttctaacct ggcagctgcc tacaaccctg actttttcga
      541 ctacaaggac ggcgttctta cttttaaagc ccccaccgga aagtaccagg ttgtttactg
      601 ggctggaacc atcaactaca tgtggctgcg gcaggataat gccgtttacc ccgatgccct
      661 ttggcttacc ggtgccggaa ttggtatgcc cacctttatt aacgatgtaa cgaatatctg
      721 gtggggatgg gatgattggg gcgtaaccac acgccaaagc tacttctttt gtccaaaaat
      781 tggcgagaat aagtaccaag ttactgttta cttgatgaat accgaggggt cgtggtggtt
      841 gcaaaccgag tttaagctct ttcctactaa gagttgggat aatgagttct cctcaatgga
      901 ttgggggtac atctccagca acttaagtcc caataccaat gggaattttg ttcacacccc
      961 cgagctaacc gaagggctgt acagaataac cattgacatg agcggcggaa aggggaacta
     1021 tttcctgatt gcccagaaga ttaactaggg ttggttttag gtttgcgtgc ttatgttgaa
     1081 gaattaacct gtttggattg gcatagtcct acaggttaat ttttttccct ccgtttgtgt
     1141 taaaatcctt tttaaaatag attgggttca gctaactttt gcacttcaac tttacaatgt
     1201 ccatctcaat tcacccgatg aaaaatatac ataacagcct attttctttt gcttccggaa
     1261 tgctgtcggc atgtttgatt ttttttgcca tccttctgcc tggctgcacg gatgattctg
     1321 acagtgtaga taatccgccc gacgacacca cccaattagg tgatgttcgg gtttgggtaa
     1381 ccacctgtaa ccaggcttac ctcttcaaag agcagtcggt ttccttttca gacgacgtgg
     1441 cgcccttcac catacgggtt gataccctta accgatacca gcaggttgaa ggttttggtg
     1501 ctgcacttac cggttcgtca gcctataacc ttaggcgtat gacagacagc caacggacaa
     1561 gcctgcttac agagcttttc gatccggtaa agggaattgg acttagctac ctgcgcctta
     1621 gcattggctc ttccgatttt tccctcagcc tttacaccta ctgcgactac ccatctattg
     1681 attcgtttgc cctgcatcct actgaccttc gcgatttgat tcctgttctt aaggaaattc
     1741 ttgccattaa tcctgctatt aagataatgg ctacaccatg gacacctcct atttggatga
     1801 aaacaagtgc tgcatggagt ggtggcagtc ttattcccga cttctacgac gagtacgccg
     1861 agtattttgt ccgctacatt agggctatgg ctggggaggg tattactatt gatgccatta
     1921 gcgtccagaa cgagcccatg tgggagtttg gtactcccgg catgaagatg acctggcagg
     1981 agcagctcac ctttattaga gatttcttag gacctaagtt tgctgcagag gatatttcga
     2041 ccaaaattct tatttgggac cataactggg atatgcccga ctaccccata agcattcttt
     2101 ccgatcctgc tgcctacatg tacattgccg gttcagcatt ccacgggtat gggggcgagt
     2161 actccgccat gctaagcgtt cacaaccagt ttccccaaaa ggggctatac tttaccgaaa
     2221 tctcgggcgg gggatgggca accgactttg acgataacct gaaatggacc attgagaatg
     2281 tattctttgg aaccctttcg tgtttttcga agaatgtgct tctatggaat ctggtgctcg
     2341 acgacaattg gggacctttc attcagggcg ggtgctccaa ctgccgtggg gttatcaccc
     2401 ttaactccga tggaagtctt acccggttca acgagtacta cgctttggcc cattttgcaa
     2461 aggcaataag accaaatgcc tacatgctgg gtacaacagt tgttggtgat atgtcccagc
     2521 tggcagactt caagtatgtt gctgctcaaa atcccgatgg ttcaaaggtt gtagttgcc
//
LOCUS       DAOOQX010000254         2579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_48645_length_2579_cov_2.579239, whole genome shotgun sequence.
ACCESSION   DAOOQX010000254 DAOOQX010000000
VERSION     DAOOQX010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48645_length_2579_cov_2.579239"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PLX53_04990"
     CDS             <1..931
                     /locus_tag="PLX53_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72470.1"
                     /translation="QRLGRKLEPFDIWYDGFKVRSGISAEKLDGLTTKKYPTVEAFDK
                     ALPEILTSLGFPKDKAQFIGDHVRVEAARGSGHAWGAEMKKDDALLRTRVPATGMNYK
                     GFNIAMHEFGHTVEQTISLHDVDYYTMHGVPNTAFTEALAFVFQNRDLAVLGISETNP
                     QKESLETLDNLWSCYEIMGVSLVDINLWKWLYNHPGASAQEVKDATINIAKDIWNKYY
                     ADVFGSTDEPILAVYSHMIDNPLYLSAYPVGHLIHFQLEKQLKGKSFASEIERIYSQG
                     RLIPQLWLKDAVGETLSVKPILEASAEALGKLK"
     gene            complement(1989..>2579)
                     /locus_tag="PLX53_04995"
     CDS             complement(1989..>2579)
                     /locus_tag="PLX53_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOY72471.1"
                     /translation="QVGSCIMPREGIFARVLKTGRLKAGDELELIPRTYRVKVVTLSD
                     RASRGDYEDRSGPEVVRLVNQFFNDIGWRHSVNSTIIPDDAATLTSTVVEAKNNGYDL
                     LITTGGTGIGPRDVAPDVVRPLLDKEIPGIMEMIRMKYGADKPNALISRSIAGVMGSM
                     FVYTLPGSVKAVGEYMQEIIKIQKHLFLMLMGIDSH"
BASE COUNT          679 a          596 c          537 g          767 t
ORIGIN      
        1 ccaaaggctg ggaaggaagc tcgaaccctt tgatatctgg tacgatgggt ttaaggtacg
       61 gagcggcatt tctgccgaga agttggatgg acttacaacc aaaaagtatc ctactgttga
      121 ggcatttgac aaggccctac ctgaaattct tacctcgtta ggctttccaa aagataaggc
      181 gcagtttatt ggcgaccatg ttcgggtcga agcagcaaga ggatcaggcc atgcatgggg
      241 tgccgagatg aagaaggatg acgctttgct tcgaacccgt gtccctgcaa caggcatgaa
      301 ctacaaggga tttaacattg ccatgcacga gtttggacac actgtggagc aaaccatttc
      361 gctccacgat gttgactact acacaatgca cggtgtcccc aacactgctt tcaccgaagc
      421 cttagccttt gtttttcaga accgcgacct ggccgttctg ggaatatcgg aaaccaatcc
      481 ccagaaggaa agcctcgaaa ccctcgataa cctttggtcg tgctacgaaa taatgggtgt
      541 ttcgctggtg gatataaacc tttggaagtg gctctacaac catcccggtg cctccgctca
      601 agaggtaaag gatgccacca tcaacattgc caaggatatt tggaacaagt actatgccga
      661 tgtgtttggc agcaccgacg agcctatttt ggcggtttac tcccacatga tcgacaaccc
      721 actttacctg tcggcgtacc cagttggaca tttaattcac tttcagctcg aaaagcagct
      781 aaaaggcaag tcgtttgcta gcgagattga gcgtatatac tcccagggaa gacttattcc
      841 ccagctttgg ctgaaggatg cggttggtga aaccctatcg gttaagccaa ttctggaggc
      901 ctccgctgaa gccttaggta agttgaagta atccggctaa gacctgaaat tttttatacg
      961 acctaacagg ctttcaatgc ctgttaggtc tttttggtta ctactactgg caagatgcag
     1021 gttcttgcac acgccggtta gggaggaggt gctagcaaat ggcaggggtg ttgcgggtgg
     1081 ctgcaaatct ggaaaaagcc ataagcaagg gcagcgtgca gacgcacaga aaaccgatag
     1141 aaggctgaca agcgacttgt tgtaaaaaat cctctcggat tcaggttgta tttttttcga
     1201 aactattgca aattgattcc taaattcatc tcacagatgt aattaaaatt gttgtccgtt
     1261 ttgcattcct ttcaacgaag caccgtcatt ttagaattac cgccaactat aaacttgttt
     1321 aggtgacgtc ggtcctgctg tagataggct agtatagcgt aggcgtttat gctatcaacg
     1381 gtaatgcata gcttgccatc tttagatttc cgtatgattt tatcttcatg tcaaaattaa
     1441 cctattactt aattcttatc aattttttat tggtatgatt aatgtttttt ataaaaataa
     1501 gtaataatta actgattagc aacgatatgg attgttgata aatttatggt tctgccgcct
     1561 ttataaccgt tttgtcttct aacctgtaat ctttttcatt ttggccatat tcaaaaataa
     1621 tgttgtgata ccgattatca ttctatatga taaatttatt aaacttttct ttgtgaactt
     1681 tgtgccttct ttgagaactt tgtggttaaa taatattaga aaccacaaag cacacaatga
     1741 tattacacaa agaacgcaat aattactatt catgattcca atatggctat cggaaaacaa
     1801 cattttcttt aaatagagcc tcccttttta tgttctcccc caacccctgc aatcctagcc
     1861 tttatctttt gctcaaatat cacaattagc tctttgatgg cgttaatcaa ttcctcctct
     1921 ttttcaactt gagaaacgat tgtcttttga acgtttaacc tacggtttat acatcattgt
     1981 tgacttcact agtggctgtc tattcccatg agcatcagga acaggtgttt ctgaattttg
     2041 atgatttcct gcatgtactc accaactgcc ttaacgcttc caggcagggt atatacaaac
     2101 atacttccca tcaccccggc aatactcctg ctgattaaag cgttgggctt gtcggctccg
     2161 tacttcattc ttatcatctc cattattccg ggaatttcct tatcaagaag tggcctaacc
     2221 acatcggggg caacatccct tggtcctata cccgttcccc cggtagtaat caggaggtcg
     2281 tacccattgt tcttggcttc aaccacagtt gaggtaagcg tagcggcatc atccggaatt
     2341 atggtggaat taacggagtg ccgccatcct atatcgttga agaattggtt aaccaaccga
     2401 accacctcgg ggccgcttct atcctcatag tcacccctgc tggccctgtc gcttagcgta
     2461 accactttca ccctgtaggt tcgtggtatg agctccagct cgtcaccggc ttttagcctt
     2521 ccggttttta agaccctggc aaaaattccc tcccttggca taatgcagct accaacctg
//
LOCUS       DAOOQX010000255         2575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_48735_length_2575_cov_2.925794, whole genome shotgun sequence.
ACCESSION   DAOOQX010000255 DAOOQX010000000
VERSION     DAOOQX010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48735_length_2575_cov_2.925794"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PLX53_05000"
     CDS             complement(<1..849)
                     /locus_tag="PLX53_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOY72472.1"
                     /translation="MEELVRLRHELHRNPELSGHESATAKRLRFFLSRYNPDELYSNI
                     AGEGMAVIYQGKEPGPTVLFRCDMDALPIAETNDVKYKSNVQGVSHVCGHDGHMTIIS
                     GLAVRFSQSKPKKGRVILFYQPSEENGEGARKSLEGLKELGLNPDYAIALHNLPGYKM
                     GSIILGRYTFSAASKGLVINLKGKPSHAAYPEHGLNPAAATAEIIQSLLSIQSTTHFK
                     NFILVTVIHIRVGDIAFGTSPGNATIMATLRAFDDDDMTLLSKIAAETATSIAHKHKL
                     CIDVSFT"
     gene            complement(1035..1538)
                     /locus_tag="PLX53_05005"
     CDS             complement(1035..1538)
                     /locus_tag="PLX53_05005"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOY72473.1"
                     /translation="MAGTIDHPIKKVLIICAKANIEDVYAALVMANGAVMEGIETKMF
                     FTFFGLDAITKKSMKKLHTSAVGNPGLKMPGGLPFPTLLGILPGVEAGVSAMMKSQME
                     KLDIPTVEEFLELITAGGGEIYACKLAVDMFGLKKEDFSEHVKDIITVGQLYELAAGE
                     GSQIIFT"
     gene            complement(1556..1867)
                     /locus_tag="PLX53_05010"
     CDS             complement(1556..1867)
                     /locus_tag="PLX53_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOY72474.1"
                     /translation="MSEKVYAGKSVTVTEDGYLTDPNQWTKDIAVEIAREEGITLSPK
                     HFEVIEFIRDKTLKGEVMTIRTIGKSGIIDTKGFYELFPGAPLKKASRIAGVSKPTSC
                     V"
     gene            complement(1885..>2575)
                     /locus_tag="PLX53_05015"
     CDS             complement(1885..>2575)
                     /locus_tag="PLX53_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72475.1"
                     /translation="GHMLIGHRAVIEQQIDALNQKLDTVIELLVSQHQKNEVVEDLID
                     DLSIVGKNAFQATVDELAVQNISIEADDLKYLFFKVLGNIKTFGELMDMLESVVDLVK
                     DLGPIVHDASIAVTEKLGQLDREGYFSYLNQLGLLAIEFKKNFTEKDITRLREEMPAV
                     GAIVRNLSQPQVVNALATITETLSEMKLDDKLDNKSLFRLFRELNKPDVRKTLSFTLR
                     LLAELGKKQDV"
BASE COUNT          632 a          606 c          586 g          751 t
ORIGIN      
        1 ggtaaacgaa acgtctatac acagcttatg cttatgggca atggaagttg cggtttcggc
       61 tgcaattttc gacagcaggg tcatgtcgtc gtcgtcaaag gctcgcaggg tcgccattat
      121 ggttgcattt ccaggggatg ttccaaaagc tatgtcgcca acccgaatgt ggataaccgt
      181 taccaggatg aagtttttaa agtgggtggt gctttgaatg gatagaagcg actggatgat
      241 ttccgcagta gccgcggcag ggttcaaccc atgctccggg taggccgcat gcgagggctt
      301 ccctttaagg ttgatgacca gcccctttga tgccgctgag aaggtgtacc gcccaaggat
      361 gatgcttccc attttgtaac ccggtaggtt atgaagggca atagcatagt cgggatttag
      421 acccagctct tttaaccctt ccagcgactt tcgggcgccc tccccatttt cctcagaggg
      481 ctggtagaaa agaatgaccc gccctttttt aggtttgctt tggctaaacc taacggcaag
      541 tccagaaata atggtcatgt gcccatcgtg cccacacacg tgtgaaactc cctgaacatt
      601 cgatttatac ttgacgtcat tggtctcggc aattggaagg gcatccatat cgcagcggaa
      661 caggacagtt ggccctggct cttttccctg gtagattacg gccattccct ctcctgcaat
      721 gttcgagtac agctcatcgg gattatacct actcaggaaa aaacgcagcc gtttggccgt
      781 ggcgctttca tgtccggaaa gttcaggatt cctatgcagc tcatgcctca aacgaacaag
      841 ttcttccatg ctcacttgta gtttttttgt ttaaaggtaa gaaaataccc cataaactca
      901 aacctatagg tatgaaaaac ctggtaattt ttattgaact cttgatgggc gggggataac
      961 gggtaaaatt gaaaaacctg tcagctattc gtagatgaca ggtttttatg ttgcccggtg
     1021 tttaccaggt catgctatgt aaagattatt tgtgatccct caccggctgc aagctcgtaa
     1081 agttgtccga cggtaataat atccttcaca tgctcgctga agtcttcctt tttaagacca
     1141 aacatatcga cggcaagctt gcaggcatat atctcgcctc caccagcggt aatcagctcc
     1201 agaaattcct ccacagtagg aatatcaagt ttttccattt ggcttttcat catggccgat
     1261 acaccagctt caacgcctgg tagtatgccc agaagtgttg gaaatggtag tccgccgggc
     1321 atttttagac caggatttcc caccgcagaa gtgtggagct ttttcatgct ctttttagta
     1381 attgcatcaa gaccaaagaa ggtgaagaac attttggttt cgatgccctc cataacagca
     1441 ccattggcca ttaccaacgc agcgtaaaca tcctcaatgt tggcttttgc gcaaattatt
     1501 agcacctttt taatagggtg atctatagtt cctgccataa ttaattgtta ttaggttaaa
     1561 cgcaacttgt tggtttcgaa actccggcaa tgcgacttgc ctttttcagg ggggcaccgg
     1621 ggaagagttc gtaaaacccc ttggtgtcaa ttatccccga ttttcctatt gtgcggatgg
     1681 tcatcacctc gccctttaag gttttatcac gaataaattc aataacctcg aagtgcttag
     1741 ggcttagggt aatgccctcc tccctggcaa tttcaacagc aatatcctta gtccactggt
     1801 ttggatcggt taggtatcca tcttcggtaa cggttaccga ttttcctgca taaacttttt
     1861 ctgacatagc gcttatggta ttagttaaac atcctgtttt ttacccagtt cagccaaaag
     1921 ccgaagggtg aatgataagg tcttcctaac atcgggttta tttaactccc tgaatagcct
     1981 gaagagcgat ttgttgtcaa gcttatcgtc gagtttcatt tctgaaagag tttcggttat
     2041 agtggccaag gcgttgacaa cctggggttg tgaaaggttt ctaactatgg ctccaacggc
     2101 tggcatttcc tcccgtaggc gtgtaatatc tttttcagta aaatttttct taaactcaat
     2161 tgctagcaat cctagctggt taagatagga gaagtagccc tcccggtcga gttgccccaa
     2221 tttttcggtt actgcaatgc tggcatcgtg aacaattggg cctaggtctt taaccaggtc
     2281 cactacactt tccaacatgt ccatcagctc tccgaatgtt ttgatgttgc ccagaacctt
     2341 gaaaaatagg tacttcagat cgtctgcttc aatactaata ttctgaacgg caagctcatc
     2401 tacggtagct tgaaaggcgt ttttgcccac aatggagagg tcgtcaatca ggtcttcaac
     2461 cacctcgttt ttttgatgtt gactaaccag cagctcgatc acggtgtcga gcttctggtt
     2521 aagggcatca atctgctgct caatgacggc ccggtggcca atcagcatgt gcccc
//
LOCUS       DAOOQX010000256         2575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_48736_length_2575_cov_2.739286, whole genome shotgun sequence.
ACCESSION   DAOOQX010000256 DAOOQX010000000
VERSION     DAOOQX010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48736_length_2575_cov_2.739286"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLX53_05020"
     CDS             complement(<1..255)
                     /locus_tag="PLX53_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HOY72476.1"
                     /translation="MRKWRIEDSAELYNINGWGVNYFSMNSNGNVVVTPKNNGVEIDL
                     RELVDELQVRDVSAPMLIRFTDILDNRIEDMSRSFEVAAQE"
     gene            complement(583..1497)
                     /locus_tag="PLX53_05025"
     CDS             complement(583..1497)
                     /locus_tag="PLX53_05025"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOY72477.1"
                     /translation="MRTKAISMIHGRHARLTRSAILFLALTCIGKVAGAQENIKPAIP
                     AKDFTTRIWLGSQLLPGYGQIANRQYWKLPVLYGGMGSMIYLGVKANQTYLSRLSAYN
                     SLTSTDPNRELYKERMVKQRQTRNLYIAAAGAFYVAGAMDAVLVYNNSKHSPAAATIL
                     STLVPGMGQAYNQKYWKIPVVYGGLATLYYLVSWNDRYYSRFKTALKLNIDGDDTTVD
                     EFGGARTADELRYYMDSYRRNRDLSILGLTAIYIINILDANVDGFLHDWNVDDNLSFR
                     VEPAIVNPALTATADTHSALGLSCRVTF"
     gene            complement(1535..2440)
                     /locus_tag="PLX53_05030"
     CDS             complement(1535..2440)
                     /locus_tag="PLX53_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOY72478.1"
                     /translation="MTKKMALGRGLGALIEDSTFESTRNSDVQTPRHAISELVQEVDI
                     ELIDPNPFQPRTNFDDESLSELAESISKLGIIQPITVRLLDGRYQLISGERRFRAAKL
                     AGLKTLPAFIRTADDQGMLEMSLVENIQREDLNAIEVAISYQRLIDECSLTQDALSER
                     VGKKRATISNYLRLLKLPAEIQLGIINSIISMGHARALISIDDASLQLKLYKKVISDD
                     LSVRQTEELVRNTVSEPQIKQSRSKVMPDLAGPELALREHLTNRLGLRADVKPGGKGD
                     GKIVISYNNPDELSVIIDKFTKIEE"
BASE COUNT          652 a          602 c          618 g          703 t
ORIGIN      
        1 ttcctgggca gccacctcaa acgagcggga catatcctca attcggttgt ccagaatatc
       61 ggtgaaccgg atgagcattg gcgcagaaac atcccgaacc tgcagctcat caaccaactc
      121 gcgcagatca atctctaccc cattattctt aggtgttacc actacattgc cgttactgtt
      181 cattgaaaag taattcaccc cccagccgtt tatgttgtaa agctcggctg aatcctcaat
      241 gcgccatttt ctcattccgg gttataatgt tatgtggtta tgaatcaacg gattaataaa
      301 tgattttatg gggctaaatt agccttaaaa tcttactata caaagggaaa gctgaaattt
      361 atttagtgca acacaagcat attaccccta ccaacatgaa ttgcggggtg aaaaatgaat
      421 aaccactagt agcacaaaac ggctactagc aaaaaatgaa cgggatgtac aagtgcattg
      481 gccagcttgt ggttgttcaa tgaaataagg aaagcacaga gggatccatt taggccatag
      541 ctatacgcct gtgtatgccc tggcttgtgg aaagtagtat agtcaaaatg taacccggca
      601 cgaaagtcca agtgctgaat gggtatcagc ggttgcggta agggcagggt taacaattgc
      661 aggctcaacc ctaaagctca ggttgtcgtc aacattccaa tcgtgaagga atccatccac
      721 gttggcgtcc agaatgttga tgatgtagat ggcggtaagg ccaagaatgc tgagatccct
      781 attccggcgg tagctatcca tatagtatcg cagctcatca gcagttctag caccaccaaa
      841 ttcatccacc gtggtgtcat caccatcaat attcagcttt aaagcggttt taaagcggct
      901 gtagtacctg tcattccagc tgacgaggta gtaaagtgtc gccaagccgc cgtaaactac
      961 aggaattttc cagtactttt ggttgtaggc ttgccccatt cccggtacca gagtggaaag
     1021 aatggtagca gctgcaggtg agtgcttgct attgttgtaa accagcacgg catccatggc
     1081 tcctgccaca tagaatgccc cggcagcggc aatatagagg ttgcgggttt gcctttgctt
     1141 caccattcgc tctttatata attcacggtt ggggtctgtg gaggtcaaac tattataggc
     1201 cgaaagcctg cttaggtagg tttggttggc ctttacccca aggtaaatca tgctgcccat
     1261 gcctccatat agaaccggta gcttccagta ctggcggtta gcaatttgcc cgtaacctgg
     1321 cagcagctgt gagccaagcc agatgcgggt ggtaaaatct ttagcaggga tcgccggctt
     1381 aatattttcc tgagcccctg ctactttgcc aatacatgta agggctaaaa acagaatagc
     1441 cgacctagtc aagcgagcat gtcggccatg aatcatggaa atagcctttg ttctcaacgt
     1501 tgcaatatta tttccgtctt gctgctttct gcctttactc ttcaattttg gtaaacttat
     1561 caataataac gctaagctca tcagggttgt tgtaggaaat gacaattttg ccatcgccct
     1621 ttccgcctgg ctttacatct gcacgtagtc cgagcctgtt ggtgaggtgt tccctcaaag
     1681 ccagctcagg cccggcaagg tcgggcatga ctttagacct ggactgcttg atttggggtt
     1741 cagaaacggt atttctaacc agctcctcgg tttgacgaac cgacagatcg tcagaaataa
     1801 ccttcttgta aagcttgagc tgaagcgagg catcgtcaat gcttatgaga gcacgggcat
     1861 ggcccatgct gattattgag ttgatgatac ccagctggat ttcggcagga agcttgagca
     1921 gcctgaggta gttggatatg gttgcgcgtt ttttaccaac cctttccgaa agggcatcct
     1981 gggttagact gcattcatca atgaggcgct ggtagctaat ggccacctca atggcgttga
     2041 ggtcttcgcg ttgaatgttc tcaacgagcg acatttcgag cattccctga tcatcagcag
     2101 ttctgataaa ggcaggaagg gttttcaaac cggcaagttt ggcagctcta aagcgtcgtt
     2161 ctcccgaaat aagctggtac cgtccatcca acaagcgaac tgtaataggt tggattattc
     2221 cgagtttgga aatggactcg gccagctcgc tcagggattc atcatcaaaa ttggttctcg
     2281 gttgaaaagg gtttgggtca atcagctcaa tgtcaacctc ctggacgagt tcggagatag
     2341 catgccttgg tgtctgtaca tcgctgtttc gggtcgattc gaaagttgaa tcctcaataa
     2401 gcgctcccaa tccccttccc aatgccattt ttttagtcat agttttatct cccagcctat
     2461 gcggtaacgg ttaatcctgg gcaatctttt gctcattctc aatctgagtc tggttgtttt
     2521 tctgaagcag ctccctggcc aggttgatgt agtttgccga gcctgtagag gccgc
//
LOCUS       DAOOQX010000257         2572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_48804_length_2572_cov_3.635280, whole genome shotgun sequence.
ACCESSION   DAOOQX010000257 DAOOQX010000000
VERSION     DAOOQX010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48804_length_2572_cov_3.635280"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLX53_05035"
     CDS             complement(<1..302)
                     /locus_tag="PLX53_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOY72479.1"
                     /translation="MNVLNSITSIKHLDSGNFFLMAGPCVVESEELAITVAEKVSAIA
                     EKFKIPYIFKASYRKANRSRIDSFSGIGDLKALKILQKIRTQLQLPVVTDIHSAEEA"
     gene            complement(321..1601)
                     /locus_tag="PLX53_05040"
     CDS             complement(321..1601)
                     /locus_tag="PLX53_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOY72480.1"
                     /translation="MSSIPLAERMRPHTLDDYVGQSHLVGEGSVLRDMVKSGNLNSFI
                     LWGPPGVGKTTLAKIVANELKRPFHILSAINSGVKEVREVIEKAKSQKFFNSPSPILF
                     IDEIHRFSKSQQDSLLGAVEQGVITLIGATTENPSFEVISPLLSRCHVYILKPLSNEE
                     LSLLLNRAISTDVELKKIKFKIAETEALYHFAGGDARKLYNIIELLASVAGGKGEVEI
                     SNEKVTKVLQENIALYDKNGEQHYDIISAFIKSVRGSDPNAAVYWLARMLEGGEDPKF
                     IARRLVILASEDIGIANPNALLLATSCFQAVHLIGMPESRIILSEASIYLATSAKSNS
                     AYEAIDKAQALVRTSGNLPVPLHIRNAPTKLMKEIGYGADYKYAHSFKGNFVDQEFLP
                     SGISGSTLYEPQDNPKEREIAMRLRNLWKDKYNY"
     gene            1802..>2572
                     /locus_tag="PLX53_05045"
     CDS             1802..>2572
                     /locus_tag="PLX53_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72481.1"
                     /translation="MKSHRRISMIIFLSTIVATFTVNAQENVVEVLSGNLNDAEKLAA
                     AYLEPFGKSLGSSLNNGWFNSAKPHGLFGFDITFTGAVTFPPSSAKNFDVAKLNLEYW
                     ELKNPSQNTSPTVSGEKVSGPILTDKATGLSELTLPKGSNMQFLPAPVIQVGLGLPFH
                     TEVVGRFFPKVDFPEVGNFSSWGIGVKNEFKEFIPGFKLIPIDVSVFFGYTEFTSTFD
                     IDKSKDQTLNFNASGFTGKLLVSKSIPVLTVYAGVGYVK"
BASE COUNT          679 a          554 c          544 g          795 t
ORIGIN      
        1 gcttcttcgg cgctgtggat atcggtaact acgggtagtt gtagctgagt tctaatcttt
       61 tgaagtatct tcagtgcttt caagtcgcca atacccgaaa atgaatcgat tctagagcgg
      121 ttggcttttc gataggaggc tttaaagatg tagggtatct taaatttctc cgctatggcg
      181 cttacttttt ctgcaaccgt tattgccagc tcctcgcttt ctacaacaca tggtccggcc
      241 attagaaaaa aattccccga atcaagatgc ttgattgagg tgattgagtt taaaacattc
      301 atatacaggt gttttacaaa tcaataatta tacttatctt tccagaggtt cctcaacctc
      361 atggcaattt ccctttcctt tgggttgtcc tgtggctcat ataatgttga ccccgatatg
      421 cctgagggca aaaactcctg atcaacaaaa tttcctttaa aactgtgggc gtacttgtag
      481 tcggcgccat agcctatttc cttcattagc tttgttgggg cattccgaat gtgcagggga
      541 acgggtaggt tgccggaagt tctaactagc gcctgagcct tgtcaattgc ttcataagca
      601 gaattactct ttgcagaggt agcaagatat atcgaggctt ctgataggat aattctagac
      661 tctggcatac caataagatg tactgcctga aagcagctgg ttgccagcag aagtgcattt
      721 gggttggcga ttccgatatc ctctgatgcc agtataacaa gtctgcgggc aataaacttt
      781 ggatcctcac ctccctcaag catacgagct agccagtaaa cagctgcgtt gggatcgcta
      841 cccctaacag attttataaa cgcagatatg atatcgtaat gctgctcgcc attcttatcg
      901 tagagcgcaa tattttcctg tagaactttg gttaccttct cattggatat ctccacttca
      961 cctttaccac cagcaacact ggctagtagt tcaataatgt tgtaaagttt tcgagcatct
     1021 cctccagcaa aatggtataa cgcctcggtt tcagcaattt taaatttgat tttttttaac
     1081 tcaacatccg tgctgatggc cctgttgagt aaaagggata gctcttcgtt gctgagtggt
     1141 ttaagtatgt aaacatggca gcgtgaaagt agtggtgaaa ttacctcgaa ggacgggttt
     1201 tcggttgtgg ccccaattag ggtgattaca ccctgctcaa cagcgcctaa aagtgaatcc
     1261 tgctgagatt tgctaaatcg gtgaatttca tctatgaaca gaattggtga gggtgaatta
     1321 aagaattttt gagacttggc cttttcaatt acctccctaa cctccttaac ccctgagttt
     1381 attgcactaa gaatatggaa gggccttttt agctcgttgg caacaatttt tgccagggtt
     1441 gtttttccca cccccggagg gccccaaaga ataaaggagt tcaggttacc cgattttacc
     1501 atatccctta gcacgctacc ttccccaacc agatgtgatt gaccaacata gtcgtctaac
     1561 gtgtgagggc gcattcgttc ggctagcggg atactgctca tgctacctta tcttttctac
     1621 aaaaatagca actaatagct taccaacgat attttttaag ttaatgatgc ttactttacg
     1681 aaacattcgt acttttgctt atgatttagg aaaataatct aacttaattt tgaaaactga
     1741 tttgattaga gctttgctgt aaaatatgcc tcccattaac cttttaaata aatgtgaaaa
     1801 gatgaaaagc catagaagaa tttcgatgat tatattcctg agcacaattg ttgccacatt
     1861 cactgttaat gctcaggaaa atgtagttga ggttttaagc ggaaacctta acgatgctga
     1921 aaaactcgca gcagcctacc tcgagccttt tggtaagagt ctgggctcaa gcctgaacaa
     1981 cgggtggttt aattctgcca agcctcatgg actttttggt ttcgatatca cctttaccgg
     2041 ggcagtaaca tttccgccat catccgctaa aaattttgat gtggccaagc ttaaccttga
     2101 atactgggag ttgaagaatc catcccagaa tacttcacct actgtttccg gtgaaaaggt
     2161 aagcggccct atacttaccg ataaggccac aggactatct gagctcaccc tgccaaaggg
     2221 ttcaaacatg caatttcttc ctgccccggt aattcaggtt gggctgggtt tgccatttca
     2281 caccgaggtg gttgggcgct ttttccccaa ggtcgatttt cctgaggtag gtaactttag
     2341 ctcctgggga attggtgtaa aaaatgaatt taaggaattt attccgggat ttaagctgat
     2401 acctatagat gtttcggttt ttttcggcta caccgagttt acctcgactt ttgatattga
     2461 taaaagcaaa gatcaaaccc ttaactttaa cgcttcgggg tttaccggca agctgctggt
     2521 ttcaaagtca attcctgttc ttaccgttta tgctggcgtc ggatacgtaa ag
//
LOCUS       DAOOQX010000258         2554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_49311_length_2554_cov_3.165666, whole genome shotgun sequence.
ACCESSION   DAOOQX010000258 DAOOQX010000000
VERSION     DAOOQX010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49311_length_2554_cov_3.165666"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PLX53_05050"
     CDS             <1..571
                     /locus_tag="PLX53_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOY72482.1"
                     /translation="HEMNSEVPEKPVFFMKPDTSLLRNNQPFFYPDFTQELSYEVEVV
                     LRVNRLGRNINRRFAHRYYDAATLGIDFTARDLQRECKQKGLPWEVAKAFDYSAPVGD
                     FIPKKELADINHLPFRLEINDRLVQAGNTSEMIFSFDELIEHVSKFVTFRTGDLIFTG
                     TPSGVGQVKIGDRLKAYIEDRLILDFFVR"
     gene            complement(641..1921)
                     /locus_tag="PLX53_05055"
     CDS             complement(641..1921)
                     /locus_tag="PLX53_05055"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOY72483.1"
                     /translation="MQPVQKFDVVVIGGGPAGLLAGGRAGQLGMKVAILEKMEKPARK
                     LRITGKGRCNITNLKSYDDFVGEIVPNSHFLAKAFGNFFSSDIINLLESNGVPTVTER
                     GQRVFPASGKAWDVAEALVGWAKRCGAQLFNHYEVESLLVSGSAITGLKVRVNKLSES
                     IVFSCKAIVVATGGKSYPATGSTGDGYRFAQQVGHTLTDLRPALTGIETHPAFSFTGR
                     LTLKNVQTTLWVDGAVAGEEFGEVELADYGLSGPAILKLSLKSVDAFRSNRKVRITID
                     LKPALTEEKLEARIIRDTSRSGYFSLEMLLRGLLPPPLVPVYLRLMNLKPKLQAALLN
                     EAERKKLVGHLKNLSFEMTGYRGWNEAIVTAGGVSLNEVDDSTMASKLVQGLFLAGEV
                     LDLTGNTGGYNLQIAFSTGWLAGQSAAAFVQKNA"
     gene            complement(1925..>2554)
                     /locus_tag="PLX53_05060"
     CDS             complement(1925..>2554)
                     /locus_tag="PLX53_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72484.1"
                     /translation="HDTGLTINLGESHGALGAEICSRYLQQKPNFTSFRNKEILTAIE
                     FHDDKSIRTEENGDALSMLNLTRLVSTADDLDAFGTIGVFRYIEIYLKRAVAANELPD
                     RVLTNLQNRYSNFKSAYALLEKFVDRQECRYYQTFNFFTRLATEVTLGVGSANGPYGV
                     YRVIKNNLVEKGQSIEDVIDYVNENPISEYAQSFFNVLKVELNINSTVS"
BASE COUNT          669 a          631 c          553 g          701 t
ORIGIN      
        1 ccatgagatg aactccgagg ttcctgagaa accggtattt ttcatgaaac ccgacaccag
       61 tcttctgcga aataaccagc cattttttta tcctgatttt acccaggagc taagctacga
      121 ggttgaagtg gtgcttaggg taaaccggct gggccgtaac atcaacaggc gttttgcaca
      181 taggtactac gatgcagcca cccttggaat cgatttcacg gctcgcgacc ttcagcgcga
      241 gtgtaaacaa aaagggcttc cgtgggaggt tgccaaagct tttgactact ccgccccggt
      301 tggtgatttc atacccaaaa aggaacttgc cgatattaac catctaccgt tcagacttga
      361 gataaacgac cgcctagtgc aggcaggaaa cacatcagaa atgattttct cttttgatga
      421 gcttatagag catgtttcga aatttgtaac cttccgaact ggcgatttaa ttttcaccgg
      481 cactccttct ggagtagggc aagtaaagat tggtgacagg ctaaaagcct atatcgagga
      541 caggctgata ctcgacttct ttgtcaggta ggcattgtcc aaaggagggg ttacgttgct
      601 ttaatcacat gctaagctgt agtcctgcga taaacttcat tcaggcattt ttttggacaa
      661 aagcagcggc cgattgccca gccaaccagc ccgttgaaaa ggctatctgg aggttgtaac
      721 cgccggtgtt tccggttaag tcaagaacct cacccgcaag aaaaaggcct tggactagtt
      781 tcgaggccat tgtgctatcg tcaacctcat ttagtgaaac tccacctgca gtaacgatag
      841 cttcgttcca acctctgtac ccggtcattt caaatgacaa atttttaaga tgcccaacca
      901 gctttttcct ctcggcttcg tttagcaatg ctgcttgtag tttcggctta aggttcatca
      961 atcttaaata aacaggcacc aggggtggag gtagcaaacc cctaagcagc atttcaaggc
     1021 tgaagtatcc cgacctggag gtatccctaa tgattcttgc ctccagcttc tcctcggtca
     1081 gggcaggttt tagatcaatg gtaatcctta cctttcgatt cgaccggaat gcatcaactg
     1141 atttcagact tagttttaaa atggcagggc cacttaagcc gtaatcggca agttcaactt
     1201 caccaaattc ttcaccagcc acggccccgt caacccaaag agttgtttgc acattcttca
     1261 gagtaagtct tcctgtaaac gaaaaagcag gatgggtttc aatgccggta agcgccggcc
     1321 tcaagtcggt tagggtatgt ccaacctgtt gggcaaacct gtagccatcg ccagttgatc
     1381 ccgttgccgg atatgattta cctccggtag ctacaactat tgctttacat gagaatacga
     1441 tagactcaga gagcttatta acccttacct taagcccggt tattgcgcta ccgcttacta
     1501 aaaggctttc aacctcatag tggttaaaca gctgtgctcc acaccttttg gcccagccaa
     1561 ccagggcttc tgcaacatcc catgcttttc ccgaggcagg aaaaacacgt tggccgcgct
     1621 cggtaacggt tggcacaccg ttcgattcca gcaggttaat gatatcgcta ctgaaaaaat
     1681 ttccaaaagc ctttgccaga aagtgcgagt tggggacaat ttccccaaca aaatcgtcat
     1741 agcttttaag gttggttata ttgcatcgcc cctttccggt tattctcagc ttacgggcgg
     1801 gcttttccat tttctcgaga atagccacct tcatgccgag ttggccggcc ctgccccccg
     1861 ccaggagacc ggcagggcct ccaccaatta ctactacatc aaatttttga acaggttgca
     1921 ttgtctaact tacggttgaa tttatattga gctcaacctt gagcacgttg aaaaagcttt
     1981 gagcatactc cgaaatagga ttttcgttta cataatcaat aacatcctca atactttggc
     2041 ccttttcaac aaggttgttt ttgataaccc tatatactcc atacgggcca ttagcgctac
     2101 caacacctag ggttacctcg gtagctaaac gggtaaaaaa gttaaaggtt tgatagtacc
     2161 tgcactcctg cctatcaacg aatttttcga gcaaagcgta ggcgctttta aagttggaat
     2221 accgattttg caagttcgtt aacaccctgt cgggcagctc attagcggcc acggctcttt
     2281 taaggtagat ttcaatatac ctgaaaacgc caatagtccc aaaagcatcc agatcatcgg
     2341 cagtggatac taggcgcgta agattgagca ttgaaagggc gtcgccattt tcctctgtcc
     2401 taattgactt atcgtcgtga aactcgattg ccgttaggat ttctttattt ctaaatgagg
     2461 taaaattggg tttttgctgt aaatatctgc tacaaatctc agcgccaagt gcaccgtggc
     2521 tctcccccaa attgatggtt aaacccgtat catg
//
LOCUS       DAOOQX010000259         2550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_49413_length_2550_cov_3.842084, whole genome shotgun sequence.
ACCESSION   DAOOQX010000259 DAOOQX010000000
VERSION     DAOOQX010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49413_length_2550_cov_3.842084"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PLX53_05065"
     CDS             complement(<1..436)
                     /locus_tag="PLX53_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOY72485.1"
                     /translation="MRINKFLLVSVVPFLMLTSCQNWFNRHLIPGKAQREEVHQRFSE
                     RLKLVEAYDTSIQRIVSYDLPLKTREGFEFLYAYMPLSDLAMHSSSYVLEQVQLALEA
                     KDHFKWGRKVPSDIFLHFVLPYRVNNEYTDSARKVFFAELKPR"
     gene            complement(900..>2550)
                     /locus_tag="PLX53_05070"
     CDS             complement(900..>2550)
                     /locus_tag="PLX53_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOY72486.1"
                     /translation="KGSIGMDVSFSAPSTDFKDLLSLIPAFYLKRYEDIQTSGQLKLY
                     GSVNGVYNDSILPSASIEIAVANAMLKYPKLPKSANGINFDAKVWFDGSNPDKSTVDV
                     NRLQFSLGGNPFDAYLHVATPVSDMQVDGKVVGKVNFATLADVIPLDSISVTGLLETN
                     LKFGGRISHLKEKEYDKFNAEGSLNISNFTLTGCDSTRQVKVGELIVNFSPKFVELQN
                     LKSSLGSSDMQLSGRLENFIPFVFRNEALQGQLNLTSTLLDANELLSGFRVQAKQPVD
                     SVVTHGVIKIPRNFNFTLSASVQHLLYGRLDISNLKGNVEADSGIVNLYNLTMNMLGG
                     DVALDGIYNPIDVSLPRVNLDLSIKDVDIPLAFKSLTSLQRIVPTFKNLSGKLSGSIS
                     IASMLDSSMMPVPSSIIASGKLQSKSIGFKESAVFGRLADVFKKEEFRSPYMSNVNVN
                     FKVNESRIAIEPFSTSVGGAKMSVGGDMGFDQSLNFKAKLAVPTELFDTAADALSKFS
                     TKTNVRVSNLELTLKISGTSYKPLVKVDWGDFTTNIDIPGL"
BASE COUNT          720 a          572 c          566 g          692 t
ORIGIN      
        1 accttggttt tagctcggca aaaaacacct tgcgggcaga atcggtgtac tcgttattca
       61 cccggtaggg cagcacaaaa tgcagaaaaa tgtctgaagg tactttcctt ccccatttaa
      121 agtggtcttt agcctccagt gccagctgta cctgctccag cacatagctg ctgctgtgca
      181 tggctaaatc gctgagcggc atgtaggcat acaaaaattc aaacccctcc cttgttttca
      241 ggggtaagtc gtaggaaaca attctttgaa tggatgtatc gtatgcctca accaacttga
      301 gccgttcgct gaagcgctga tgaacttctt ccctttgagc tttgcccgga attaagtggc
      361 ggttgaacca gttctggcat gaagtcagca tcaaaaatgg cacaacacta acgagtagaa
      421 acttattaat ccgcataatt taccaatatt aagattatca gcatgctatt gaaacatatt
      481 cactctcggg gataatatat aaataaaaat gaagcacctc tccattcaaa tctgggacaa
      541 tacctagcaa acactattat cctgtaaatt aaagggaaaa actataatgg ctaagcaaat
      601 ataatctatt tgcagctgga aaaacaataa ggtggttaaa aagaccttga tctagccata
      661 tttaatataa cctggctggc atacggaaaa ttatgcaggg ctttttacgg aaagcatgat
      721 gtgcgttttt ttaggttgac tatgaacacc ccacgtttgc aaagggtaaa ccaaaaccct
      781 tatcaggttc tttaggcatc tcatcaccaa agcaggtcat gcctctggtt tagtgttgta
      841 ttgcccacca ggtaaaatca attctaaact ttttttgtct gcgtgtactc aatccagaac
      901 tacaacccgg ggatgtcaat gttggtagta aaatcgcccc aatcaacctt aactaggggt
      961 ttataggaag taccggaaat tttaagggtc aactccaagt ttgacaccct aacatttgtt
     1021 ttggtagaaa acttgctcag cgcatcggca gcagtgtcga acaactcggt aggcactgcc
     1081 agcttggctt taaagttaag agactggtcg aaacccatgt cgccgcctac actcatttta
     1141 gcaccaccca cgctggtgct aaagggttca atggctattc tgctttcgtt aaccttaaag
     1201 ttaacattta cattgctcat gtaagggcta cggaattctt cctttttaaa aacatctgcc
     1261 aacctcccga aaactgcaga ctccttaaag cctatgctct tggactggag cttaccgctg
     1321 gctataatgg agctgggcac gggcatcatg gatgagtcga gcatggaggc aatgctgata
     1381 cttcccgata gcttacccga aaggttcttg aaagttggaa caatacgctg tagtgaggtt
     1441 aatgatttaa atgccaacgg gatatcaacg tccttaatgg acaaatcgag attaactctg
     1501 gggagactaa catcaatggg attatagata ccatccagag ccacatcacc tccaagcatg
     1561 ttcatggtta ggttgtacag gttgactatg ccgctgtcag cctctacatt tcccttcagg
     1621 ttcgaaatgt cgagtctgcc gtagagtagg tgctgaacgc ttgcgctcaa agtaaagtta
     1681 aagttgcgag gaattttaat tacaccatgg gtaacgaccg aatcaaccgg ctgtttagcc
     1741 tgaactctaa atcccgatag cagctcgtta gcatccaaca gggttgatgt gagattcaat
     1801 tgaccttgca gggcttcatt cctaaaaacg aacggtataa aattttccag cctaccgctt
     1861 aactgcatat cgctgcttcc cagacttgat ttaaggtttt gcagctcaac gaattttggt
     1921 gaaaagttga cgattagctc acctacctta acctgccggg tagaatcaca tccggtaaga
     1981 gtgaagttgg aaatgttaag ggaaccttca gcgttgaact tgtcgtactc cttttccttc
     2041 aggtgtgaaa tacgtcctcc aaacttcagg ttggtctcca gcaggccggt aacggaaatg
     2101 ctgtcgaggg gaataacatc ggccaaggta gcaaagttaa ccttcccaac aaccttgcca
     2161 tcaacctgca tgtcgcttac aggcgtggcc acatgaaggt aggcgtcgaa ggggttgccg
     2221 cccaggctaa actgaagcct gtttacatca actgttgatt tatcggggtt tgaaccatcg
     2281 aaccaaacct tagcatcaaa gttgatcccg ttggctgatt ttggtagctt ggggtatttg
     2341 agcatggcat tggccactgc aatttcgatt gatgcgctgg gcaaaatgga gtcgttgtaa
     2401 accccgttaa cgcttccata aagtttcagc tggccggagg tttgaatatc ctcgtagcgc
     2461 tttaggtaga atgccggtat cagggaaagc aggtctttga aatcggttga aggagcgcta
     2521 aagctaacat ccataccaat gctgcctttg
//
LOCUS       DAOOQX010000260         2550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_49428_length_2550_cov_2.747495, whole genome shotgun sequence.
ACCESSION   DAOOQX010000260 DAOOQX010000000
VERSION     DAOOQX010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49428_length_2550_cov_2.747495"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /gene="yedF"
                     /locus_tag="PLX53_05075"
     CDS             <1..396
                     /gene="yedF"
                     /locus_tag="PLX53_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HOY72487.1"
                     /translation="TQPDKRAEEYCDVSPKPDVNSGFIVLLNSATMGKGNDELGKILM
                     KGFLSTLLELKATPTEIICYNSAVTLALKSSDTAATLQQISERGIKITLCGTCVDFYN
                     LRDDLAVGQISNMLYIAQRLSSGVRIIQP"
     gene            476..1600
                     /locus_tag="PLX53_05080"
     CDS             476..1600
                     /locus_tag="PLX53_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOY72488.1"
                     /translation="MNLYFDNSSTSFPKPEGVSQSINEYFSHGGTYGRAAYNRVIEVS
                     RKVESTRSLLANLIGTSLAPNVVFTSNATHAINLFIRGMRYPKRRVIISPLEHNAVCR
                     PLQYLFQTAGVGVDIFPHHPDGRIDIQKAAKVNFASYDLGIINHVSNVNGVIQPIKEI
                     KRLMGNTPLLVDASQSAGRVEIRADEWKLDALALTGHKGIMGPTGTGALFIRNPDMAD
                     PLIFGGTGSNSESYLQPIDLPDRFEAGTPNIVGIYGLWGALSSGRGYSFKREHFVELL
                     HEISTFSSFKLLAADDTDFQSDVFSIKPLGMEVSELARILFEKFSVETRSGLHCAPLA
                     HRTLGTFTTGAVRFSLSKFHTDNDLDYLHSCLKKIDEGRS"
     gene            1584..1823
                     /locus_tag="PLX53_05085"
     CDS             1584..1823
                     /locus_tag="PLX53_05085"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOY72489.1"
                     /translation="MREEANTGFTILIFSNIHQVMHAEKIIADKGYPYRIVPVPSNLS
                     SECGLCIRIESSYLDSILSDLQNIKVEIHTLNNEP"
     gene            1853..>2550
                     /gene="selD"
                     /locus_tag="PLX53_05090"
     CDS             1853..>2550
                     /gene="selD"
                     /locus_tag="PLX53_05090"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOY72490.1"
                     /translation="MMEEFDLLSTVEQGGCSSKIPAALLESLLPALPKLVDENILVDF
                     SSHDDAGVYRISDDFALILTTDFFPPVCSDPFEFGQISAANSISDVYAMGGEPFLALN
                     ISMFPSSRIPIDVYKSILEGAASKALEANVAIIGGHTIDDFPPKFGLAVVGRVDPKRL
                     VTNSGLRPGDKLILTKPLGTGITVAAKRLKMVTDESYRIAIQWMKTLNDRACSVMKKY
                     HVTGATDITGFGLLG"
BASE COUNT          705 a          583 c          549 g          713 t
ORIGIN      
        1 actcaaccgg ataagagagc agaggaatac tgcgatgttt ctcccaaacc tgatgtcaat
       61 tccggtttta tcgtactgct gaatagcgcc acaatgggta agggaaacga cgagttgggt
      121 aaaattctta tgaaagggtt tctcagcact cttctggagc ttaaagctac accaaccgaa
      181 ataatctgtt ataatagtgc ggtaaccctt gcccttaaaa gttccgacac tgctgcaact
      241 cttcaacaaa tttctgaaag gggtataaaa attactcttt gtggaacctg cgttgacttc
      301 tacaacttaa gggacgattt ggccgtgggc caaatttcca atatgctcta tattgcccaa
      361 aggctgtcat cgggtgtgag gataattcaa ccctaatatt attcatatca tttttatttc
      421 tagttgatta ccttattaaa ctgaaagtag ttaattaggt agattaagta ataaaatgaa
      481 cctgtacttc gataactcat caacaagctt ccctaaaccc gaaggggtta gccaatccat
      541 caacgaatac tttagccatg gtggtaccta tggaagagca gcctacaatc gtgttatcga
      601 ggtttcccgt aaggttgaat ctacccgtag cctattggcc aaccttattg gaaccagcct
      661 tgcccccaat gtggttttta cttcaaatgc cactcatgct attaacttgt ttatcagagg
      721 aatgcgatac cccaagagga gggtaattat ttctccactt gagcataacg ccgtttgcag
      781 gcctctacaa tacttattcc agactgcagg ggtcggagtt gatattttcc ctcatcatcc
      841 cgatgggaga attgatattc aaaaagccgc taaggttaac tttgcaagct acgatttggg
      901 aattatcaac catgtaagca acgtgaatgg ggtaattcag cccataaagg agataaaaag
      961 gttaatgggc aacaccccac tcctggttga cgccagccag tcggccggga gggtcgagat
     1021 aagagctgac gaatggaagc tagatgcttt agctcttacc ggccataagg gtatcatggg
     1081 gccaactgga accggtgccc tattcatccg caatcccgac atggccgatc ctttaatttt
     1141 cggtggaaca ggatcaaatt ccgagagcta cctgcaaccc atcgacctac ccgataggtt
     1201 tgaagccgga acacctaata tcgtcggaat ctacggttta tggggagctt tatcatcggg
     1261 taggggctac tcttttaaac gcgagcactt cgtagagctc ctgcacgaaa tttctacatt
     1321 ttcttccttt aaacttttgg cggctgacga cacggacttc caatcggacg tcttctccat
     1381 taaacccctt ggaatggagg tgtcagaact tgctcgcatt ctctttgaaa aattctcggt
     1441 tgaaacccga agtgggctac actgtgcacc tcttgcccat cgcacgttgg gaacttttac
     1501 taccggagcc gtacggtttt ccctgtcaaa gtttcatact gataacgacc tcgactactt
     1561 gcatagctgc ttaaaaaaaa ttgatgaggg aagaagctaa cacaggattt acaattctca
     1621 tattcagcaa catccaccag gtaatgcatg ccgaaaagat tattgctgac aagggttatc
     1681 cgtacagaat agtccccgtt ccatcaaacc tcagctcgga atgtggttta tgtataagaa
     1741 ttgaaagttc atatttggat agcattctat cagatttaca gaatattaag gtcgagattc
     1801 ataccctaaa taatgaacct taaccacctt taaaaagaat ataaattaca agatgatgga
     1861 agaatttgac ttactatcaa ccgtggagca ggggggatgt tcctccaaga ttcctgctgc
     1921 attgctcgaa tcccttcttc ccgcattgcc aaagcttgtt gatgaaaaca ttctggttga
     1981 ttttagctcc catgatgatg ccggggttta ccggatttca gacgattttg cgctgattct
     2041 aactaccgat tttttccctc cggtttgttc cgatcctttt gagtttggcc aaatatccgc
     2101 agcaaattct attagcgatg tatacgcgat ggggggagag ccattcctcg cccttaacat
     2161 ttctatgttc ccctcttcca ggatacccat tgatgtttac aagtctatac ttgaaggggc
     2221 cgcttcaaaa gcgctggagg caaatgttgc aatcatcgga ggtcatacca ttgatgattt
     2281 ccccccaaaa tttggcttgg ctgttgttgg ccgtgttgat cctaagcggt tggttaccaa
     2341 ctccgggctt cgccctggtg ataagctgat tctcaccaag cccctgggaa ccggcattac
     2401 cgtagcggca aaacggctta aaatggttac agacgaatcg taccgaattg ccattcaatg
     2461 gatgaaaaca ttaaacgaca gagcctgcag cgttatgaaa aaatatcatg ttaccggggc
     2521 aaccgatatt accggctttg ggcttttagg
//
LOCUS       DAOOQX010000261         2544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_49584_length_2544_cov_3.804741, whole genome shotgun sequence.
ACCESSION   DAOOQX010000261 DAOOQX010000000
VERSION     DAOOQX010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49584_length_2544_cov_3.804741"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PLX53_05095"
     CDS             <1..641
                     /locus_tag="PLX53_05095"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOY72491.1"
                     /translation="FNLLWLYWFGRLFLQHLSQRQLVGVYLLGGITGAIFFIAAYNIL
                     TVFRAISSEAMALGASASVLAIVIAVSTLKPNNSIYILFLGEVKLKYLALATIIIDII
                     SIPVSNPGGHIAHLGGAVFGFSFIKLLNRGTDLTKFLNIFSRVKKPKAHRSKNLKVSY
                     RRPETDYEYNRRKANEQKSIDRILDKIAKSGYDSLSKEEKETLFRMKNDSVN"
     gene            691..1728
                     /locus_tag="PLX53_05100"
     CDS             691..1728
                     /locus_tag="PLX53_05100"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOY72492.1"
                     /translation="MFALGLLFCYASVYINPARFWIPAIVGLFYPFFLLANIAFLTYW
                     IIVWKKELLISLLAILLGINHLSAFVQLRPGNKHESAVPSIRLISYNVNLFRLYPWSK
                     LPPSYDGVIRYIGSNNFDIACLQEFYTVSTKFSEADAKRKLGMRSKIGYIIQNSRSAY
                     GLAIFSRFPIVNSGEIKFENTLNSSIYADVKINGDTVRVYNLHLQSLRLKEHNFNFLL
                     NNELRQGNDRYDEIMDLASRLRIAFIKRSQQVELVNRHLKNCPYPVILCGDFNDTPVS
                     YTYRELTGKLHDSFKEAGAGMLNTYAGILPSYRIDYILHSPEFKTLKFSSPRLNLSDH
                     YPVCANFTLTH"
     gene            complement(1718..>2544)
                     /locus_tag="PLX53_05105"
     CDS             complement(1718..>2544)
                     /locus_tag="PLX53_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOY72493.1"
                     /translation="QKVGAFKYRGATHAVLKLNDEEARKGVATHSSGNHAAALALAAA
                     NRGIPAYIVMPENAPAIKKKAVAGYGGIITYCRSTQQDREETLRVVQQQTGATLIHPY
                     NNINVICGQGTAALELLDEMPDLDVVIAPVGGGGLLSGTATFIKGQYPHVKVFGAEPI
                     NADDAFRSLRDGKIYPSLSPSTIADGLLTSLCPLTYSILRSKCDGIFTVSEKGIVNAM
                     RLIWERMKLVVEPSSAVPVGVIAEHPEAFRGKRVGIIVSGGNADLDHLPWLDQSNG"
BASE COUNT          657 a          652 c          566 g          669 t
ORIGIN      
        1 tattcaacct gctgtggttg tactggtttg gaaggttgtt tctacagcat ctctcgcaaa
       61 ggcagctggt gggagtttac ctcctgggag gcattacggg agcaatattt tttatcgcag
      121 cctacaatat tctaacggta ttcagggcta tatcgtccga agctatggct cttggagcat
      181 cggcatcggt tttggcaatt gttatagcgg tttcaaccct taagcccaac aattcaattt
      241 acatcctctt tctgggtgaa gtaaagttga agtacctggc gctggccacc ataatcatcg
      301 acatcatcag cattccggtt tcaaaccccg gtgggcacat cgctcacctg ggaggagcag
      361 tattcggctt ctccttcatc aagctgctta acaggggaac tgatttgaca aaatttctca
      421 acattttcag ccgggtgaaa aaacccaaag cccatcggag caaaaatctc aaggtttcct
      481 accgccgacc cgaaaccgac tacgagtaca accgccgaaa ggccaacgag caaaaatcta
      541 tcgatcggat tctcgacaaa atagccaaat caggctacga cagcctgagc aaggaggaaa
      601 aggaaaccct gtttcgaatg aaaaacgact cggtgaacta gcatggccaa gggcggaatt
      661 ctacataaag taattgttgc agcaaacatt ttgtttgcgc taggtctgct attctgctat
      721 gcgtcggttt acatcaaccc cgcccggttt tggatacctg ccattgtagg cctattctac
      781 ccatttttcc tgcttgccaa catagccttc ctgacctact ggatcattgt atggaaaaaa
      841 gagctgttaa tttcgctact cgccattctg ctaggaataa atcaccttag cgcttttgtc
      901 cagctacgcc ctggcaacaa gcatgagtcg gctgtcccct ccatcaggtt gatttcatac
      961 aatgtaaacc tgttcaggct ctacccatgg tctaaacttc caccctcata tgatggggtg
     1021 attcgctaca tcggcagcaa caacttcgat atagcctgcc ttcaggagtt ttacacagta
     1081 agcactaaat tttcggaggc cgatgctaaa aggaagctgg gcatgcgctc caagattggg
     1141 tatatcattc aaaatagccg ttcggcatac ggtctggcaa tcttttcaag atttccgata
     1201 gtaaattcgg gtgaaattaa atttgagaac accctcaact cgtccatcta tgccgacgtt
     1261 aaaatcaacg gcgataccgt gagggtttac aaccttcacc tgcaatcgct caggcttaag
     1321 gagcacaact ttaactttct gctaaataat gaattaaggc aggggaatga ccggtatgac
     1381 gaaattatgg atttggcttc gaggctaaga atcgctttca tcaagcgttc ccagcaggtt
     1441 gagctggtta accggcacct gaaaaactgc ccttatccgg ttattctttg tggcgatttt
     1501 aatgataccc ccgtttcata cacctacagg gagctcacgg gtaagctgca tgattcgttt
     1561 aaagaggcag gtgccggaat gctaaatacc tatgccggca ttctaccttc gtaccgtata
     1621 gactacatcc tgcattcgcc tgaattcaaa actttaaaat tttcaagccc gcgtcttaac
     1681 ctttccgacc actaccctgt ttgtgcaaat tttaccctta cccattagac tgatccagcc
     1741 agggcagatg gtcgagatcg gcatttccac ctgagacaat tataccaact cgttttcccc
     1801 taaaagcctc tgggtgctca gctataacgc ccaccggaac agctgatgag ggttcaacca
     1861 caagcttcat acgctcccag attaaccgca tggcgtttac tatacccttc tcggataccg
     1921 taaaaatgcc atcgcacttc gaccggagaa tggagtaggt aagcgggcaa agcgaggtga
     1981 ggagcccatc ggcaatggtg gatggactga gcgaagggta aattttccca tcacgaagcg
     2041 accgaaaggc atcatcagca ttgattggtt cggctccgaa cactttcacg tgcgggtatt
     2101 gcccctttat aaaggttgct gtgccgctga gcaatccgcc accaccaacc ggtgctataa
     2161 ccacatccag gtcgggcatt tcatcgagca gctccaacgc agctgttccc tgcccacaga
     2221 taacgtttat gttgttgtag gggtgaatga gggttgcacc ggtttgctgc tgaacaaccc
     2281 taagcgtttc ctccctgtcc tgctgcgttg atcggcagta ggtaatgata cctccgtaac
     2341 cggctactgc ttttttctta atagcagggg cattttcggg catcacaata taggccggaa
     2401 tacctctatt tgcagcagca agcgcaaggg cagccgcatg gttgcctgaa ctgtgggttg
     2461 caaccccttt tctagcctcc tcatcattca actttaaaac tgcatgggtt gcacccctgt
     2521 acttaaatgc tccgactttt tgaa
//
LOCUS       DAOOQX010000262         2540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_49701_length_2540_cov_3.246680, whole genome shotgun sequence.
ACCESSION   DAOOQX010000262 DAOOQX010000000
VERSION     DAOOQX010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49701_length_2540_cov_3.246680"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLX53_05110"
     CDS             complement(<1..563)
                     /locus_tag="PLX53_05110"
                     /inference="COORDINATES: protein motif:HMM:NF016635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HOY72494.1"
                     /translation="MTNDKSIRISKLAREFNVGVSTLVEFLKKKGYHVTPDPNAKVDE
                     DIVNLLTKEFGAEVNIKKESQKVSLKSLRDAKETLSIDEIEKKRDEDEGDSDFSNDEV
                     IIKTTGFVHEKIDDFGMEKPKIDLKVKGKIDLDNLSSQPRSSKKETPVESTTSEVPVT
                     PLKQATKEVATKKESKEDEGAKPERTKV"
     gene            complement(624..1862)
                     /gene="nusA"
                     /locus_tag="PLX53_05115"
     CDS             complement(624..1862)
                     /gene="nusA"
                     /locus_tag="PLX53_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOY72495.1"
                     /translation="MENINLVDTFAEFKELKNIDRPTMMSVLEDVFRATLIKLYGSDD
                     NFDIIINIDKGDFEIWHNREVVEDGKVEDPGRQIALSEARKIDADFEVGEEVTSEVKF
                     ADFGRRAILALRQNLTSRIMELEKNNLYNKYKDRVGDLITGEVYQVWKKEIMVLDDEG
                     NELVLPKTEQIPTDFYHKGDSIRAVVYKVEMKNNTPQIILSRTANAFLERLFELEVPE
                     IFDGLITIKKIVRIPGERAKVAVESYDDRVDPVGACVGMKGNRIHGIVRELRNESIDV
                     INYTNNTQLFIQRALSPAKITSIKFDETTKRAEVYLKPSEVSLAIGKGGYNIKLASQL
                     TGYEIDVYRESEGEDEEDVNLDEFADEIEGWIIDTLKGIGCDTAKSVIEIPFKELVKR
                     TDLEEETVKEILKILKSEFE"
     assembly_gap    1964..1973
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1974..2365)
                     /gene="rimP"
                     /locus_tag="PLX53_05120"
     CDS             complement(<1974..2365)
                     /gene="rimP"
                     /locus_tag="PLX53_05120"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOY72496.1"
                     /translation="MISRETIEAIVAEKLEEENLFIVDISISPKNTITIMLDGEKGVS
                     IKSCVAISRLVESKLDRNVEDYGLEVTSAGLGQPFRVVRQYSINIGREVEVLLLDGNK
                     FKGVIVEAGSDNFSIEYSEKVLMDDGKH"
BASE COUNT          606 a          642 c          476 g          806 t
ORIGIN      
        1 accttagtcc tttcaggctt ggcaccctca tcttccttac tctctttttt tgtggccacc
       61 tctttggttg cttgtttaag gggagtaacg gggacttcgc ttgttgttga ctcaacgggg
      121 gtttcctttt tagaactcct gggctgggag gataggttat ccaaatcaat tttcccctta
      181 acttttaaat caattttcgg tttctccatt ccgaaatcat caattttctc atgaacgaaa
      241 ccggtagttt tgataattac ctcatcgttg gaaaagtcgc tatccccttc atcctcatcc
      301 ctcttttttt ctatttcgtc tatagataag gtttcttttg catcgcgaag cgatttgagg
      361 ctcaccttct gggattcctt tttaatatta acttcagcgc caaactcctt ggtgagcagg
      421 ttaacaatat cctcatcaac cttagcgttt ggatcggggg taacgtggta gccctttttc
      481 ttgagaaatt ccacaagtgt agaaaccccc acattaaatt cccttgccag cttacttatt
      541 cgtattgatt tatcgttcgt catacaggtg gttcccgttg tttatgttag aaatgctcgt
      601 gtaaagctac atgaaagaca atactattca aattcggatt ttaatatttt aagaatttcc
      661 ttgaccgttt cctcttccaa atcggttcgt ttcaccagct ccttaaaggg aatttcaatc
      721 acgctcttgg cggtgtcgca gccaatgccc tttagggtgt caataatcca cccctcaatt
      781 tcatcagcaa actcatcgag attcacgtct tcctcgtcct caccttccga ttcgcggtaa
      841 acatcaattt catagccggt gagctggctg gcgagcttaa tattgtaccc gcccttacca
      901 atcgccaggg aaacctcgct tggtttaagg taaacctcgg ctcgtttggt tgtttcgtca
      961 aacttaatcg aggtgatttt agcggggctt agggcgcgct gaatgaacag ctgagtattg
     1021 ttggtgtagt tgataacgtc gatgctctcg ttgcgaagtt cccgaactat accgtgtatg
     1081 cggtttccct tcattccaac acaagcgcca acggggtcaa ccctatcgtc gtacgactca
     1141 actgcaacct tggcgcgctc gccgggaata cgaacaatct tcttgatggt aatcaggcca
     1201 tcgaaaattt caggaacctc cagctcgaat aggcgctcga ggaaggcatt ggcagttctg
     1261 gaaaggataa tttgcggggt gttgtttttc atctccacct tgtacaccac ggcccttatt
     1321 gagtcaccct tgtggtagaa atcggttggt atctgctctg ttttgggcag caccagctcg
     1381 ttaccctcat cgtccagaac catgatttct ttcttccaga cctggtaaac ctcaccggtg
     1441 ataaggtctc caactctatc cttgtactta ttgtagaggt tgttcttttc gagctccatg
     1501 attcgtgaag tgaggttttg gcgcaatgcc aggattgccc ttcgcccgaa gtctgcaaac
     1561 ttcacctcgg aggttacctc ctcgcccacc tcaaagtccg catcaatttt tctagcttcc
     1621 gatagggcta tctgccttcc tggatcttcg accttgccgt cctcaaccac ctcgcggttg
     1681 tgccaaattt caaaatcgcc cttatcaatg ttgataataa tatcgaagtt atcgtcagac
     1741 ccgtaaagct ttataagcgt cgccctaaaa acatcctcca gaacgctcat catggtaggc
     1801 ctgtcaatat tttttaactc cttaaactcc gcaaatgtgt caactaggtt aatgttttcc
     1861 attgtgctga ttatttatca tatttactat ttaaacgaaa tgaccgcttt ggttgacttt
     1921 accacattgt agggaaacga ctttcgctgc tgccccacct gttnnnnnnn nnnttgtgct
     1981 taccatcatc cataagaact ttctcgctat actcgatgct aaaattatcg ctaccggcct
     2041 ccacaataac gcctttgaac ttgtttccgt ccaaaagcaa aacctctacc tccctgccaa
     2101 tattgatgct atactgccgg acaaccctaa acggctgtcc taaccctgca gaggttacct
     2161 ctaacccata atcttcaacg ttcctatcga gcttcgactc gaccaaacgg ctgattgcca
     2221 cgcatgattt aatactaaca cccttttcac catcaagcat gatggttatg gtatttttgg
     2281 gacttatgga gatatcaaca ataaaaaggt tttcctcctc cagcttttct gcaactatcg
     2341 cttcaattgt ttcccggcta atcattattg ctacatctaa taaaacaggg gactctgtcc
     2401 cctgccacct gcttaaaaac tgttgcaaat atacagcttt ctaacattct ttcaaaatat
     2461 tatgctgaat ttatgaattt tatcgggtta gttctattta aagtgcgcac cagcatagca
     2521 actagtaacg ttttcaaatg
//
LOCUS       DAOOQX010000263         2533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_49890_length_2533_cov_2.715093, whole genome shotgun sequence.
ACCESSION   DAOOQX010000263 DAOOQX010000000
VERSION     DAOOQX010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49890_length_2533_cov_2.715093"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..979
                     /locus_tag="PLX53_05125"
     CDS             227..979
                     /locus_tag="PLX53_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOY72497.1"
                     /translation="MPSKEKLVIIPTYNELENIDKMLARVMELEGDFNVLIIDDASPD
                     GTAERVKAIQQNFPGRVSLIERPGKLGLGTAYICGFKWALANGYEYIFEMDADFSHNP
                     DDLVNLYKACRDGADIAIGSRYINGVNVVNWPIGRVLMSYMASKYVRLITGMRIMDAT
                     AGFVCYKGSVLKAINLDKIKLVGYGFQIEMKFTAWKLGYKIVEVPIIFTDRKFGVSKM
                     SGGIFNEALWGVIAMKLKSIFKPYQKLNLPER"
     gene            1062..2432
                     /locus_tag="PLX53_05130"
     CDS             1062..2432
                     /locus_tag="PLX53_05130"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008021808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOY72498.1"
                     /translation="MGRIAVAGATIINEGNRFQGTVIINRGIIEEIITGASTQLTRYN
                     PEHTIDARGLLLIPGVIDEHVHFREPGLTSKGDIYSESKAAAAGGVTSIMDMPNTIPQ
                     ATNLELLDDKISTAAEKSLVNYSFYMGATNDNIEQLVKIDPLKACGIKLFMGSSTGDM
                     LVSNREALEKIFKLSPTIIVAHCEDEASIRQNAEKVKQEKGENIPFRFHSSIRSAEAC
                     MISSMLAVELAKKHGSRLHILHLSTARELSLMNNSIPLADKKITSEACIPHLWFSERD
                     YDRLGWRIKCNPSIKLESDRQALLEGISSGLVDAVATDHAPHLAFEKDSTYFKSASGM
                     PMVQHSLQVMLELSKKGYFSVEKVVEKMCHAPATIFRIDRRGYIRKGYHADLVLINPT
                     STETVTTQSLHYRCGWSPLEGEIFHNSIETTFINGHIVYHKGKFDETIKGEALSFTQN
                     QLAYQG"
BASE COUNT          817 a          458 c          555 g          703 t
ORIGIN      
        1 ggctgcaaat ataccacctc ccccaattcc atccaaactt ttttacacct tttttacatc
       61 tttttaaaat aaaatttatt ttggatgttt aattatttgg ttctcatata atttaatttt
      121 gtctgatttt gaaaaccaga tggattctct caaaccaaat gcataaaagc attatattcg
      181 catattcaca ttacatatgc caaccctaac ttaataatac ataacaatgc ccagtaaaga
      241 aaagttagta attattccga cctataatga acttgagaat atagataaga tgctagccag
      301 ggtgatggag cttgaggggg attttaatgt tttaattatt gatgatgctt cacccgatgg
      361 gacagcagaa agggtaaaag ccattcagca gaatttccca ggtagggttt cattgataga
      421 gaggccgggg aaattaggat tagggactgc ttacatttgt ggtttcaagt gggcgcttgc
      481 caatggttac gagtatatat ttgaaatgga tgcggatttt tcacataatc ccgacgattt
      541 ggtcaatctt tataaggcct gcagggatgg tgccgacata gcaattggtt caagatacat
      601 aaatggtgta aatgtggtta attggccaat tggcagagta ctcatgtcgt atatggcatc
      661 gaaatatgta aggttgataa ccggaatgag aattatggat gctacagcag gctttgtttg
      721 ctataaggga agcgttttaa aggctataaa ccttgataaa attaagcttg ttgggtatgg
      781 ctttcagatt gaaatgaagt ttaccgcatg gaagctgggg tataaaattg tggaggtacc
      841 tataatattt actgatagaa aatttggagt ttcaaaaatg agcggaggca ttttcaacga
      901 ggctctttgg ggagtaattg ccatgaagct aaaaagtata ttcaagccct accaaaagtt
      961 gaatctacct gagcgatagc aacttctata tttaaaatac tactagcatt agagtttttg
     1021 gaatattagt ggtgttgcat aaaagcgtta aaagaattat gatgggacga attgcagtag
     1081 ctggtgccac gattatcaac gaggggaata ggtttcaagg gacagtaata ataaatagag
     1141 gaataataga agaaataatt actggagcct caactcaact aacgcgttat aatccagaac
     1201 atacaataga tgccagggga ttgctactaa ttccaggggt tatagacgag catgttcatt
     1261 ttagggagcc gggattaacc agtaaagggg atatatacag cgaatcgaaa gcagcagcag
     1321 caggtggggt tacctctata atggacatgc ccaacactat tccccaagcc accaatcttg
     1381 agcttcttga tgataaaatt agtacagctg ctgaaaaatc gctggttaac tattcatttt
     1441 acatgggggc aacaaatgat aacattgagc agctggtaaa aattgatcca ttgaaagcat
     1501 gtggcatcaa gctatttatg ggctcgtcaa ccggagacat gttggtgagc aaccgggaag
     1561 cactcgaaaa gatttttaag ctgtcgccta caatcattgt tgctcattgc gaagatgagg
     1621 ccagcatacg tcaaaatgcg gaaaaagtca agcaagaaaa aggagaaaac attcctttta
     1681 gatttcactc cagtattcgc agtgccgaag cctgtatgat ttcttcaatg ctagcagtag
     1741 agttggccaa gaagcatggt tcaagacttc atattcttca tctttcaaca gccagggagc
     1801 tttcgcttat gaataatagt attcctcttg ctgataaaaa aattacctca gaagcatgta
     1861 tccctcacct atggtttagc gaaagggact atgacaggtt gggatggagg ataaaatgca
     1921 atccaagtat taagctggaa tccgacaggc aggctcttct ggaaggcata agtagtggtt
     1981 tggttgatgc cgtggcaaca gatcatgctc cacatttagc cttcgaaaag gatagtacct
     2041 actttaagtc agcctcagga atgccaatgg ttcagcattc actacaagtt atgctggaac
     2101 tttcgaaaaa ggggtacttt tcagtagaaa aggtggttga gaagatgtgc catgctccag
     2161 ccaccatttt caggattgac cgcagaggct atatccgaaa gggctaccat gccgatttgg
     2221 tgctgattaa tccaacatct accgaaacag taactaccca atcacttcac tacaggtgtg
     2281 gttggtcacc gttagagggt gaaattttcc ataacagcat tgagacaacc tttataaacg
     2341 gtcatattgt ctatcataaa ggaaaatttg acgaaaccat taaaggcgaa gccttaagct
     2401 tcacccaaaa ccaactggct tatcagggtt gatgaaaggc caacagcatt gaaaacctcc
     2461 aacgtaatat agagctacaa gttcaaccca tcaccagtag ggtcaacacg ctcaagcctt
     2521 attccggtaa aat
//
LOCUS       DAOOQX010000264         2529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_50007_length_2529_cov_3.474535, whole genome shotgun sequence.
ACCESSION   DAOOQX010000264 DAOOQX010000000
VERSION     DAOOQX010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50007_length_2529_cov_3.474535"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..818
                     /locus_tag="PLX53_05135"
     CDS             177..818
                     /locus_tag="PLX53_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOY72499.1"
                     /translation="MGQSGEAKMRFFDMAVKGGWIMIPIFILSIIAVYIFVERYMAIH
                     KASKIDANFMNKIREYIHEDKVEAALALCQSQNTPVARMIEKGIQRIGRPLSDVNSAI
                     ETAGNLEISKLEESMPLLATIAGGAPMLGFLGTVIGMIRAFFDMSMAGNSIEIGLLSN
                     GIYIAMVTTVAGLIVGIPAYFGYHYLVARIEKVVYTMEANISEFMDLLNEPVA"
     gene            830..1234
                     /locus_tag="PLX53_05140"
     CDS             830..1234
                     /locus_tag="PLX53_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOY72500.1"
                     /translation="MALRHRSKSDPGLNMSSMTDIVFLLLLFFMISSTLVHPNALRLL
                     LPQSNSQISDKPVTSVSITADRNFYLETVPVSIGQLEYLLQQKMKGENDPTIALHVDR
                     SVPMEDVVKVMNIAKDNGYKLVLATQPIKKEQ"
     gene            1238..2074
                     /locus_tag="PLX53_05145"
     CDS             1238..2074
                     /locus_tag="PLX53_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOY72501.1"
                     /translation="MTITANKAKGIAATVLFHILMVLLLMMFGFNIPFPLPAEEGIII
                     NFGNSDEGMGLEEPAIGEQAISNPAGYPTMQEDESPLTQDYEEAPALPVKKEESKKKV
                     AEPTEKTNPASSTDKVAPSTTAATPEVEKPREANAKALFPGQKINGGQTGGEGVTGKP
                     GNQGSLEGSASSTNRTGGATGGGGDGQNGIITNLNGRSALLLPKPDYPKQKEGRVVVE
                     VTVDNHGNVTKAIAGIKGSTTLDTDLLKAAEKAALSAKFDVSSNAPSFQVGTITYIFR
                     LN"
     gene            complement(2083..>2529)
                     /locus_tag="PLX53_05150"
     CDS             complement(2083..>2529)
                     /locus_tag="PLX53_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOY72502.1"
                     /translation="RIIGILLVFMFFLLLFEPEKWLKPKNPDSRIRFTPFRFIVFFVI
                     GIYGGFIQAGVGFFLLAGLVLGAGMDLVRSNALKVLITMVFTILALVVFIINGQVDFK
                     LGIIMGIGNMIGAWIATSVALKHGASFIRWVLLICVLAFGLKLLIW"
BASE COUNT          767 a          615 c          556 g          591 t
ORIGIN      
        1 tccatttgtc cagttcaggt agcacgccca accccattat ttgtgggtta ttaatttagc
       61 ctaaacaata ccctggtcat tttaaacgtt tttttagtaa ttttcgcaaa attaaatttc
      121 aagcaatgac tacactcacc actattcttc tatcggcagc tcaggcccaa acgggtatgg
      181 gacaaagcgg cgaggctaaa atgagatttt ttgatatggc cgttaagggc ggttggatta
      241 tgattcccat ttttatcctc tccattattg ccgtttatat ttttgttgaa cgctacatgg
      301 ccattcataa agcttctaaa attgacgcca actttatgaa caagataagg gagtacattc
      361 atgaggataa agttgaagcg gcactagccc tttgccaatc gcaaaacaca ccagttgccc
      421 gaatgattga aaaaggaatt caacgcatag gcaggccctt atcggatgta aactcggcaa
      481 ttgaaactgc cggtaacctg gagatttcca aacttgaaga gagcatgcca ttgcttgcca
      541 ccattgccgg tggtgcccca atgctagggt ttcttggcac agttattggt atgattcgtg
      601 cgttcttcga catgtccatg gcgggcaaca gtatcgaaat aggcctttta tctaacggta
      661 tatacattgc catggttacc accgttgccg gattaattgt agggattcct gcttacttcg
      721 gctaccatta cctggttgca cggatcgaga aggtggttta caccatggag gcaaatattt
      781 cggagtttat ggatctgctt aacgaacctg tagcataaaa cgttacatca tggcattaag
      841 acatcgttca aaatccgatc caggccttaa catgtcgtcc atgaccgaca tcgtgttcct
      901 gctgctgcta ttcttcatga tctcctctac cctagtacac cccaacgccc tgaggctgct
      961 acttccacaa agcaacagcc agatttccga caaacccgtt acctcggttt ccattacagc
     1021 cgaccgcaac ttttacctcg aaaccgttcc ggtatccatt ggccagctgg agtaccttct
     1081 tcagcagaaa atgaagggag aaaatgaccc taccatagca ctacatgttg acaggtcggt
     1141 tccgatggaa gatgtggtta aagtgatgaa tattgccaag gataatggat acaagctggt
     1201 tttagctacc caaccaataa aaaaggagca gtaacaaatg acaatcacgg ccaataaggc
     1261 aaagggaata gcagcaaccg ttctgttcca tatccttatg gttctccttt taatgatgtt
     1321 tggctttaac atacccttcc ccctaccagc cgaagagggg attattatta actttggcaa
     1381 ctccgacgaa ggtatgggat tggaggaacc agcaattggc gagcaggcaa tttcaaaccc
     1441 ggcgggatac cccaccatgc aggaggatga aagtccccta acacaggact acgaggaagc
     1501 cccagcctta ccggttaaaa aggaggagag caaaaagaaa gtagctgaac caacggaaaa
     1561 aaccaacccc gccagtagca cggacaaagt ggctccatcc acaacggcag caactcccga
     1621 agtcgaaaag ccacgggagg caaatgccaa ggcgcttttc ccaggacaaa aaattaatgg
     1681 cgggcaaacc ggaggagagg gagttacagg caagccgggc aaccagggat cgcttgaggg
     1741 ttctgccagc tcaaccaacc gcaccggcgg agcaaccggt ggaggcggtg acggacagaa
     1801 tggaattatc accaacctta atgggagaag tgcgctgctt cttcccaaac ccgattatcc
     1861 caagcagaag gagggtcgtg tagtggtgga agtaactgtt gataaccatg gaaatgttac
     1921 aaaagctatt gctggtataa agggttcaac cactcttgac accgatctac taaaggctgc
     1981 cgagaaagcg gcgttgagtg ccaaattcga cgtatcgtca aacgccccat ccttccaggt
     2041 tggaactatt acctatatct ttaggctaaa ctagcacaaa ggctaccaga ttaacagctt
     2101 gaggccaaag gccagcacac aaatcagcaa cacccaccga ataaatgatg caccatgttt
     2161 tagcgcaacg ctggtagcaa tccaggcacc aatcatgttg ccaatgccca tgatgatgcc
     2221 cagcttaaag tcaacctgtc cgttaatgat aaacaccacc aacgccaaaa tggtgaatac
     2281 catagttatc aaaaccttaa gagcattcga tctaactaaa tccatacctg cacccagcac
     2341 cagccctgcc aaaaggaaga acccaacgcc agcctgaata aatcctccgt agattcctat
     2401 tacaaaaaag acaatgaacc gaaacggagt aaagcgaata cgactatcgg gatttttagg
     2461 tttaagccat ttttcgggtt caaaaagcag aaggaagaac atgaatacta gcagaatgcc
     2521 tataattcg
//
LOCUS       DAOOQX010000265         2528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_50028_length_2528_cov_3.853619, whole genome shotgun sequence.
ACCESSION   DAOOQX010000265 DAOOQX010000000
VERSION     DAOOQX010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50028_length_2528_cov_3.853619"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..495
                     /gene="ruvX"
                     /locus_tag="PLX53_05155"
     CDS             73..495
                     /gene="ruvX"
                     /locus_tag="PLX53_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOY72503.1"
                     /translation="MGRILALDIGQKRTGIAVTDPLKIIANGLTTIPSHTVLGFLADY
                     FSKESVELLVVGSPKQMDNSSSEAMKFIDPIVRGIRKTFPGMPIQFIDERFTSKMAKQ
                     AMIDGGMKKMDRRNKAMVDTISATIILQSYLESLSFNR"
     gene            564..1118
                     /gene="def"
                     /locus_tag="PLX53_05160"
     CDS             564..1118
                     /gene="def"
                     /locus_tag="PLX53_05160"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOY72504.1"
                     /translation="MILPIYVYGSSILRKVAQDINPDYPNLKRLIDDMFETMSQSDGI
                     GLAAPQVGLSIRLIVIDGTPLAKDDPSLKDFRKVIINPYIEERFGEKVIYSEGCLSIP
                     NIHEEVEREEKVRISYLDENFTAHNEVFEGFAARIVQHEYDHLEGILFTDRLSPIRKQ
                     LLRSKLGAISKGKFTADYKCRLVK"
     gene            1396..1971
                     /locus_tag="PLX53_05165"
     CDS             1396..1971
                     /locus_tag="PLX53_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72505.1"
                     /translation="MDKKLVFKDVLSNGVSTGLKNFLSLIGCVVLWLLTVWIPYINVG
                     TTIAIITLPAALSKGAVISPLEIFNKRYFRYMGEFFLVEGLAGIIIFSAMLFMFFPAI
                     VMSIAYSFGALLVVDKGKGASEALRLSNKATYGNKWTIFWVQLVLILPMFIFAWIWRP
                     LAIIYAILYVPFTLGATAFIYKELTGDISEE"
     gene            complement(2066..>2528)
                     /locus_tag="PLX53_05170"
     CDS             complement(2066..>2528)
                     /locus_tag="PLX53_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOY72506.1"
                     /translation="ARITDWEKYSLLDGEANIFFENTFVGATVLDISRATDTLQVSLG
                     RDKGISINREKIKDYTTRQLVGTKKEETRTWRTTVKNNKLQKISITVMDQVPVSTSEE
                     IEVNILETSGGKVNTENGEVTWELTLEPGAEKKLDLKYSVKYPKNKVLVIE"
BASE COUNT          669 a          521 c          557 g          781 t
ORIGIN      
        1 agctgtagat gtcgggttaa aagcaggcct ggagaggtgc ctggaaaaat atattaaatt
       61 ggggaaagag tagtgggacg aattttagca ctcgatattg ggcaaaaacg aacgggaatt
      121 gcggtaacag atcctttgaa gataattgcc aacgggttga ctaccatacc ctcccatacg
      181 gtactcggtt ttttagccga ttactttagt aaggaaagtg ttgaacttct ggtagttggt
      241 agccctaaac agatggataa ctcttcatct gaggctatga agtttatcga tccaattgtg
      301 cgcgggatta gaaagacttt tcctggtatg cctattcagt ttattgatga gcgctttacc
      361 tctaaaatgg caaagcaggc catgattgat gggggcatga aaaaaatgga tcgccgcaac
      421 aaagctatgg ttgacaccat tagcgcaaca attattcttc agtcgtatct cgaaagcctt
      481 tcgtttaatc gttaggaggg cttaggttaa acttttttga aataggcttg ttaaaaaatg
      541 atgtacagct ttatttattt gctatgattc ttcctattta cgtttacggc tcgtcaattc
      601 ttagaaaagt ggctcaggac ataaaccctg actaccctaa cctgaaaagg ttgattgacg
      661 atatgtttga aaccatgagc cagtccgacg gaattggcct ggcagcacct caagttggcc
      721 tatctataag gttgatagta attgatggta ctccgctagc taaagatgac ccctccctaa
      781 aggattttag aaaggtgatt atcaacccat acattgagga acgttttgga gaaaaggtga
      841 tttacagcga gggatgcctc agcattccga atatccatga agaggtagag cgtgaggaga
      901 aagttcggat tagctatctg gacgaaaatt ttaccgctca caacgaagtt tttgaaggct
      961 ttgctgctcg cattgtccag cacgagtacg accacctgga gggaatcctc tttaccgatc
     1021 gcctttcgcc tatccgcaag cagctgctga gatcaaagct gggtgcaata tctaagggta
     1081 aattcaccgc cgattataag tgccgtctgg ttaagtagcg ttgcctgcaa tctatcgatt
     1141 actggcaact ttaatttgta aataatggca atggtgattg cccaaatatt ctatattttt
     1201 ttcggtttat ttcgacggta atctacatcc actattgtag gataatatcc tttacatgct
     1261 cacgcaatca attgcagaac ccgagttgtt aatgcattcc tatttgatta acaaattttg
     1321 tattcaggta gtattgttta tttaacaagt tgattgtaaa tttatttggt tatttcaaac
     1381 taaaaccaaa caactatgga caagaaactt gtattcaagg atgtgctatc caacggtgtt
     1441 agcacggggc taaagaactt tctttcgcta atcggctgcg tggtgctctg gttgcttacc
     1501 gtttggattc cctacataaa cgttgggaca accattgcca ttatcaccct tcccgcagca
     1561 ctgagcaagg gtgcagtcat ttcacccctc gaaatattta ataaaaggta ttttaggtac
     1621 atgggtgaat tcttcctggt ggaggggctt gcaggcatta taattttttc ggcaatgctt
     1681 ttcatgtttt tccctgccat tgtgatgtca attgcttaca gctttggcgc tcttctggta
     1741 gttgacaagg gaaagggcgc ttcggaggcc ttacggctta gcaacaaggc tacctacggg
     1801 aacaagtgga caattttctg ggttcagctc gtactcatcc tccccatgtt catctttgcc
     1861 tggatttgga gaccgttagc cattatctat gccatacttt atgtgccctt cacccttggc
     1921 gcaactgctt ttatttataa ggagcttact ggtgatatta gtgaggagta ggatagctgt
     1981 aaaagattct tgcagctaaa aagaaaaacc ccgtcgtgat gagaaggggt ttttcttagc
     2041 aacaacttaa aggtatgaac agaacctact caataaccag aaccttgttt ttcggatact
     2101 ttacggaata cttcaggtcg agtttctttt ctgctcccgg ttccagcgtc aactcccagg
     2161 taacctctcc attttctgta tttaccttgc ctcccgaggt ttccagaata tttacttcaa
     2221 tctcctcaga ggttgatacc ggaacctggt ccatcacagt tatgcttatt ttctgaagct
     2281 tattattttt aacggtagtg cgccatgttc gtgtttcctc ctttttggtg cctaccagct
     2341 ggcgggtggt gtagtcttta attttttcgc ggttaatgct aatgccctta tccctaccaa
     2401 gtgatacctg aagggtgtct gttgccctgg agatatcgag aacagtagcg ccaacaaaag
     2461 tattctcaaa aaatatgttg gcctcaccat cgagcaggct atacttttcc cagtcggtta
     2521 ttctggcc
//
LOCUS       DAOOQX010000266         2520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_50254_length_2520_cov_3.209736, whole genome shotgun sequence.
ACCESSION   DAOOQX010000266 DAOOQX010000000
VERSION     DAOOQX010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50254_length_2520_cov_3.209736"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="PLX53_05175"
     CDS             complement(<1..1530)
                     /locus_tag="PLX53_05175"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOY72507.1"
                     /translation="MSKVKRVYTFGNKKAEGKADMKNLLGGKGANLAEMCLLGLPVPA
                     GFTITTDTCNEYYQNNHELPSDLIPDIWEAMKNIEKIMGMKFGDPENPLLVSCRSGAR
                     KSMPGMMDTVLNIGLCSSTIPGLIKKTNNPRFVWDSYRRLIMMYADVVMEKAEGVEPT
                     DGKGIRKLLDDILDEYKNSKGYKDDTQLTAEDLQHLAEEFKKQVKKSLGKEFPDDAKE
                     QLLGGIKAVFKSWNGKKAVSYRRIEGIPDDWGTAVNVQAMVFGNMGNSSATGVAFTRN
                     PATGENVFYGEWLINAQGEDVVAGIRTPNPLNNETKNDQNKHLPSMEELMPDTYKELV
                     AVRNTLEKSFKDMQDIEFTIQEGKLYMLQCRTGKRTGTAALNMAMDMLHEGLIDEKTV
                     VLRVEPAQLDELLHPICDPEEEKKVNPIVKGLPAGPGAAVGKVVFTAEDAVAAARRGE
                     SVILVREETNPEDVEGMRAAVGILTARGGMTSHAALVARGWGKCCIVGAGAMLVNSSE
                     KI"
     gene            complement(1934..>2520)
                     /locus_tag="PLX53_05180"
     CDS             complement(1934..>2520)
                     /locus_tag="PLX53_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY72508.1"
                     /translation="LLIGILLGSLAGFLRGWVDSAIMWFVNVVWSVPTLLMVIALTMV
                     IGKGFWQIFVAVGLSMWVDMARVVRGQVLSVRENGYVEAATVLGLSRFRVIFRHILPN
                     IMGPVAIVCASNFATAILLEAGLSFLGIGVQPPMPSWGSMIRDHYGYIIMDKAYLAIL
                     PGFAIMMLVLAFNLLGNSLRDALDPQTIRNNSPK"
BASE COUNT          612 a          649 c          553 g          706 t
ORIGIN      
        1 aattttttcg gatgaattaa ccagcatggc gcctgctcca actatgcagc attttcccca
       61 accgcgtgca accagagcag catgtgaggt cattccgcct ctggctgtaa ggatgccaac
      121 tgcagccctc ataccttcaa cgtcttcggg gttggtttcc tcgcgaacca ggataacact
      181 ctcgcctctt cttgctgcag caacagcatc ttcagcggta aataccacct tcccaacagc
      241 tgctccagga cctgccggta gtcccttcac aatggggtta acttttttct cctcttccgg
      301 gtcgcagatg gggtgcagca gctcgtcgag ctgggctggt tccacgcgta gcacaacagt
      361 tttttcgtca attaagccct cgtgaagcat atccatggcc atgttcaggg cggctgtccc
      421 ggtgcgcttt cccgtacggc actgtagcat gtatagtttg ccttcctgaa tggtgaattc
      481 aatatcctgc atgtccttga acgacttttc gagcgtgttg cggacagcca caagctcctt
      541 gtaggtatcg ggcattagct cctccatgct tggaaggtgc ttgttttggt cgttcttggt
      601 ttcgttgttc aacgggtttg gtgtacggat gccggcaacc acatcttcac cctgagcatt
      661 gataagccat tcgccataga atacattttc accggttgcg gggttgcggg tgaaggcaac
      721 accggttgct gagctgttgc ccatgttccc aaataccatg gcctgaacgt ttactgctgt
      781 tccccagtcg tctggaattc cctcaatcct gcggtacgaa acggccttct taccgttcca
      841 gctcttaaaa acagctttaa ttccgcccag cagctgctcc ttggcatcat ctgggaattc
      901 cttacccaac gatttcttta cctgcttttt aaattcttca gccagatgtt gaaggtcttc
      961 tgcggtgagt tgggtatcgt ccttgtaacc cttgctattc ttgtactcat caagtatatc
     1021 gtcgagaagc tttcgtatgc cctttccgtc ggtaggctca acaccttcag ccttttccat
     1081 taccacatca gcgtacatca taataagccg gcggtaagaa tcccatacga accttggatt
     1141 atttgttttt ttaatcaatc cagggatggt tgacgagcaa aggcctatat ttagcaccgt
     1201 gtccatcatc ccgggcattg acttcctggc acctgagcgg cagctgacaa gcaacggatt
     1261 ttcgggatcg ccaaacttca ttcccatgat tttttcgata ttcttcatgg cctcccatat
     1321 atcgggaatg aggtcagagg gtagctcatg gttattctgg tagtactcgt tacaagtatc
     1381 tgtagttatg gtaaaccctg caggaactgg aagccctagt aggcacattt cggccagatt
     1441 tgcccccttt cctcccagca gatttttcat atctgcctta ccttcagctt ttttgttacc
     1501 aaaggtataa actctcttaa ctttcgacat ttatcaatca ggttttaatt agacttttag
     1561 ttatgtataa gaagcacaaa ttgaaaatct tagacaactt acaaaagtat taaaaaattt
     1621 tggacgtgat tttctgtttc cttttgagcg caacattttc ttgcaatcgt ttgaatgagc
     1681 cggctatcgg gtaggtgaat atgtaagctt gcttgtaagc cgagaggttg agagcagcta
     1741 aaaactaacc ctgaaaagtt cgacaggtgt tacagtgagc agttcggcag actcactgta
     1801 acacctgttg agtggagaat ccgccggtag gcggacaagt tccgccagca ggcggacagg
     1861 ttcttgaatc cgtcaggagc cggacaagtt tttgaatcct gcttgaattt ttttatcctt
     1921 tatccttatt atttcatttg gggctattgt tcctgatggt ctggggatca agggcatccc
     1981 taaggctgtt gcccaacagg ttaaacgcca ggaccagcat cattatggca aaaccgggca
     2041 gtatagccag gtatgcttta tccatgatga tgtagccgta gtgatcgcga atcattgagc
     2101 cccatgaggg catgggaggt tgaacaccaa ttcccaaaaa gctaaggcct gcctccagca
     2161 aaattgcagt agcaaagttg ctagcacata caattgccac tggccccata atgtttggga
     2221 gtatgtgcct gaaaattacc ctaaacctgc ttaatcccag aacggttgct gcctcaacat
     2281 agccattttc tctgaccgac aaaacctgcc ccctcacaac acgagccata tcaacccaca
     2341 tgctaaggcc tactgccaca aatatttgcc agaaaccctt accaataacc attgtcaggg
     2401 cgattaccat gagtaaagta ggaactgacc aaaccacgtt aacaaaccac atgatggcgc
     2461 tatcaaccca acccctcaga aatccggcca gcgaacccag tagtattcca ataagcaggg
//
LOCUS       DAOOQX010000267         2519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_50277_length_2519_cov_3.360390, whole genome shotgun sequence.
ACCESSION   DAOOQX010000267 DAOOQX010000000
VERSION     DAOOQX010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50277_length_2519_cov_3.360390"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1334
                     /locus_tag="PLX53_05185"
     CDS             114..1334
                     /locus_tag="PLX53_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72509.1"
                     /translation="MKEPNRVGWILLVAGSWGIMRFATDNETYIVPIFFSLLGGFFFL
                     RGTERSGKGSIFWSGFFSAFAILLHQVMIFWWIAIATGVIVRNRKREIFQFFCPALTV
                     PAAYLIAVWLEYGSIRVPVIVKFGLSDYYNGAAKLEFNGRVLLMWLVSLFRSFFQAHG
                     YMFNLFSQSWTWVIAGISAVMLTGYGLTNAIKNGKIQSSAFKRPVWIFALAALLHIFF
                     VLLSGGNAEFMAMLPALLAIVLSVIIDINPKALVITALGMLAWNITIGLIPLKYYTLD
                     GSSMLVNSILANPQPSKSAYLLHSEQRIKNELEYRQISQKPVLLGRARYNTQQLTDSL
                     EVLLEEGFAVYTDDYLRPQTVSREYIVEDHPASDDLWAPFRLIPCDSVVTLTGIYSLY
                     RVLPPVASSLHPER"
     gene            complement(1309..2172)
                     /locus_tag="PLX53_05190"
     CDS             complement(1309..2172)
                     /locus_tag="PLX53_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOY72510.1"
                     /translation="MKHIVVPIDFSKESLNGLRLAIIFANRFGSDIQMVHVQKFPSEY
                     TPGQQEEDRRNKEKKFMELVEEYSPKMLESANLTYILKKGKVYSEVVNQAQAFEDSAI
                     ICSTHGASGFEEFFIGSNAFRIISATDRPVITIRHGSMPRDIQKIVLPIDITTDTRQK
                     VPVTADIAKAFDAEIHVVAVASSQSDEIDLKLNAYSRQVTTYLKNREVRFQTSSLRGD
                     NITNITIEYALDVNADLISIMTEQNFSFSDFVLGSQAQQMLSKSPIPVLSVTPKEIFI
                     MGSFNVQGGGY"
BASE COUNT          631 a          579 c          581 g          728 t
ORIGIN      
        1 caggcttcag catcaagcag aattaacatt cgacttgcca aaatgcccag gaaaacgctg
       61 cttgctgtta tattgcctta ccttactccc gccaactccc ctcatctcac tgggtgaaag
      121 aacccaacag ggtaggctgg attctacttg ttgccggtag ttggggaatt atgcgttttg
      181 caaccgataa tgagacctat atagttccca tatttttctc gctgttggga ggttttttct
      241 ttctaagggg aaccgaacgt tcagggaaag gcagtatttt ctggtctggt tttttttcag
      301 catttgccat tctgctccac caggtaatga ttttttggtg gattgcaata gcaacgggag
      361 ttatagttcg aaatagaaaa agggaaatat tccaattttt ctgccctgct ctaactgtgc
      421 cagccgccta cttaattgcc gtttggctgg agtatggcag catcagggtt cctgtgatag
      481 tgaaatttgg gctatcagac tactacaatg gagctgcaaa acttgagttt aacggcaggg
      541 tgctcttgat gtggttggtg agcttgtttc gctcgttttt ccaggctcat gggtacatgt
      601 ttaacctgtt tagtcaaagt tggacatggg tgatagcagg aatttcggcc gttatgctaa
      661 ccggatacgg cctaaccaac gctattaaga atgggaaaat ccaaagttca gcatttaaac
      721 ggccagtttg gatttttgca ttggctgctc tgctgcacat tttctttgtt cttttatccg
      781 gtggtaatgc cgaattcatg gccatgcttc ctgctctact agccattgtg ctttcggtaa
      841 taatcgatat caacccaaaa gccttggtaa ttacggctct gggaatgtta gcatggaata
      901 ttaccatcgg actcattcct ctaaaatact acacgcttga tggcagcagt atgttagtta
      961 acagcatcct tgcaaatcct caaccatcaa agtcggctta cctgctacat agcgagcagc
     1021 ggatcaaaaa tgagctggag tacaggcaaa tttcgcaaaa accagtttta ttgggcagag
     1081 ccaggtataa cacccaacag ctaaccgata gtctggaggt attgctggag gaaggttttg
     1141 ctgtttacac cgacgactac ctgcgaccgc aaacagtatc gcgtgagtac attgttgaag
     1201 atcatccggc atctgatgat ttgtgggctc cctttcgcct aataccctgc gattcagttg
     1261 ttacgctaac tgggatctac tcgctttacc gtgttttacc tcctgttgct agtagcctcc
     1321 accctgaacg ttgaagctac ccatgatgaa aatttccttg ggtgtaacgc ttagcacagg
     1381 aatagggctt ttgctgagca tctgctgggc ctgactaccc agcacaaaat cgctgaagct
     1441 gaagttctgc tcggtcatta tgctgataag gtcggcatta acatcaagag catactcaat
     1501 ggtaatattg gtgatattat cgcctcgtag gctggatgtt tgaaagcgga cctccctgtt
     1561 tttcaggtag gttgtaacct gccgggagta ggcgttgagc tttaggtcaa tttcgtccga
     1621 ctgggaggag gcaacggcaa caacatgaat ttcggcgtca aaagccttgg caatatcggc
     1681 cgttaccgga actttctgcc gggtatcggt ggttatatca attggaagca cgattttttg
     1741 gatatccctg ggcatggagc catgcctgat ggttataacc ggcctgtccg tagccgaaat
     1801 aatacggaaa gcgttgctcc caatgaagaa ctcttcaaac cccgaagcgc catgggttga
     1861 gcatattata gccgaatcct caaaggcctg tgcctgattg accacctcgc tgtaaacttt
     1921 tccctttttt aggatgtaag tgaggttggc actttcgagc attttgggtg agtactcttc
     1981 aaccagctcc ataaactttt tctccttatt ccgacggtcc tcctcctgct gtccgggggt
     2041 gtattcggaa gggaacttct gtacgtgcac catttgaata tcgctgccaa accggttggc
     2101 aaatatgatg gccaaacgaa gaccgttaag agattccttc gaaaaatcga tggggactac
     2161 aatgtgcttc atggcttaag ggttcaggtt aaacgtaagc aaatctacgt caaattattc
     2221 cgttaaccaa tgcgacaggt caaatttatt ttacatcagg gttggaataa ccataggctc
     2281 aggattacgc aaagttccgt aaatcgccca caacctcatt gctgccgcct gctgcaaatc
     2341 gtttcgaata gtagctgcaa aaaacctgct tgcctgaacc cttcacccta attaatggaa
     2401 tagagatcat tcacactaga ccccgccaag ctaggccttt cctgaaagaa tcctttctcc
     2461 ctccagtatt ctctgcagat ggcagaaaag tagctctccg ttttgctcat ttcgtaggt
//
LOCUS       DAOOQX010000268         2518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_50324_length_2518_cov_3.078360, whole genome shotgun sequence.
ACCESSION   DAOOQX010000268 DAOOQX010000000
VERSION     DAOOQX010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50324_length_2518_cov_3.078360"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..677
                     /locus_tag="PLX53_05195"
     CDS             327..677
                     /locus_tag="PLX53_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOY72511.1"
                     /translation="MSTQILIPFFITAVFLVAAGITLAYMLSPKSFNLQKQEPYECGI
                     PTVGVTWLQFNVGYYTFALLFLIFDVETVFLFPWAVVMKQIGLVGLVEILIFFFILVL
                     GLAYAWKKHALVWQ"
     gene            668..1255
                     /locus_tag="PLX53_05200"
     CDS             668..1255
                     /locus_tag="PLX53_05200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HOY72512.1"
                     /translation="MAIKSMKESDFKDTDTLDQQMLEENGIFFTTVDSILNWGRSNSI
                     WPLTFATSCCGIEMMATAAARHDFARFGIEVYRASPRQADVIVVAGTIVNKMAPVLRR
                     LYDQMAEPKYVVAMGACAVSGGPFFYNSYHVLKGVDHLIPVDVYIPGCPPRPEAFLYG
                     IMQLQRKIKKETIKNKYARKNVANNYDEKNPLPEK"
     gene            1262..1723
                     /locus_tag="PLX53_05205"
     CDS             1262..1723
                     /locus_tag="PLX53_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOY72513.1"
                     /translation="MTNQQLEEVVKRLGVEAVISEAQFLTVEVPFDKLFDLARNLKEN
                     EQTQMDYLILITGVDYVSSFGCTYLLTSTTHNHYMVLKTRNIDKANPRVDSVTSLWQT
                     AEYHEREIYDLMGITFNNHPDMRRMFLDESWVGHPLRKDYVDESNIIERKY"
     gene            1728..>2518
                     /locus_tag="PLX53_05210"
     CDS             1728..>2518
                     /locus_tag="PLX53_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOY72514.1"
                     /translation="MAEEFVHLAVAGTEGQDLEINMGPQHPSTHGVMRFVVKLKGEVV
                     ESVQPHCGYIHRGIEKMCESLSYPQIIHLTDRMDYLSAHINNEAVCLAVEKALNIEVS
                     DRIKYIRTIIAELNRIASHLLWWSSMCMDMGAVTPYFYAFRDREKILDIFEETTGGRL
                     LYSYNCPGGLMNDIHPNFQKRVKEFIPYFRKSFKEYDELVTGNVIFQNRTMGIGLLTR
                     EQAIAYGVTGPAGRGSGFACDVRKRAPYSAYDKVQFNEILYNEGD"
BASE COUNT          691 a          557 c          595 g          675 t
ORIGIN      
        1 atatatccat tgagtaagct tgatggtttt acattaagtc tccaatgcag aagggagtat
       61 tgaaaacctt tcgcaataga gagcaaatct taggctaagc tcttacatcg ttaaaaactt
      121 atttttgtta tccagttatg ttaatagata gatataaaaa taaaaaaatg tgaattttag
      181 ttaaatcata tacccaaaaa atctactttt gtggttgcaa aaaaaatgtg ttgggtctcc
      241 atcctgaaat atttaagatt gatagcaact caatttacta tttactactc ggataaattt
      301 gctgaacaac ctcatacatt aatggcatga gcactcaaat tttaattccc tttttcatta
      361 cagccgtttt tctggtggct gccggaatca cactggccta catgttgtcg cccaaatcgt
      421 ttaacctgca aaaacaagag ccctacgagt gtggaattcc cacggttggg gttacgtggc
      481 tccagtttaa tgttgggtac tacacctttg cgctgctctt cctgatattc gatgtggaaa
      541 cagttttcct gtttccctgg gcagtggtaa tgaagcaaat agggttggta ggtctggttg
      601 aaatcctgat attctttttt atcctggtgt tgggtttagc ctacgcctgg aaaaaacacg
      661 ctttggtatg gcaataaaat caatgaagga gtctgatttt aaggataccg ataccttaga
      721 tcagcaaatg ctcgaggaga atggaatctt ttttactact gttgacagca tacttaactg
      781 gggtcggtcc aattcaatat ggccgctcac ctttgccacc agctgttgcg gaatcgagat
      841 gatggccacg gcggcagcac gccacgattt tgcccgcttt ggcattgagg tttacagggc
      901 atcgccgcgt caggccgacg ttattgtggt agccggaacc attgtcaaca agatggcacc
      961 ggtgctcagg cgcctctacg accagatggc cgaacccaag tatgttgtgg ccatgggggc
     1021 atgtgctgtt tcgggaggcc cattcttcta caactcatac catgtgctca agggggtcga
     1081 tcatcttatt cctgttgacg tgtacatacc cggttgccca cccaggcccg aggcttttct
     1141 gtacggcatc atgcagctcc agcggaaaat caagaaggag accataaaga ataagtacgc
     1201 caggaaaaat gttgccaaca actacgatga gaaaaatcct ttacccgaaa aatagcaaac
     1261 catgaccaac cagcagctag aggaagtagt aaaacgcttg ggtgttgaag ccgtaatatc
     1321 ggaagcacag ttccttaccg tggaggtgcc gttcgacaag ctctttgatt tggccagaaa
     1381 ccttaaggaa aacgagcaaa cccaaatgga ctacctaatc ctgattaccg gcgttgatta
     1441 tgtgagtagc tttggatgca cctacttgct tacctccacc acccacaacc actacatggt
     1501 tttaaaaacg aggaatattg ataaggcaaa tccccgggtc gattccgtta cctctctctg
     1561 gcaaacagcc gagtaccatg agcgtgaaat ttacgacctg atgggtatca ccttcaataa
     1621 tcatcccgat atgcgccgaa tgttcctgga cgaaagctgg gtagggcacc ccctccgaaa
     1681 ggattatgtt gacgaaagta atatcatcga aagaaaatac tagctcaatg gcagaagaat
     1741 ttgtacatct tgcggttgcc ggtaccgagg ggcaggattt ggaaatcaac atgggtcccc
     1801 agcatccttc aacccatggc gttatgcgtt ttgtggtcaa gctgaagggc gaggttgtgg
     1861 aatccgttca gccgcattgc gggtatattc acaggggtat tgagaagatg tgcgaatccc
     1921 tttcttaccc ccagatcatc catctgaccg ataggatgga ttacctttcg gcccacatca
     1981 acaacgaggc ggtttgcctg gctgttgaaa aagctttaaa tatagaggtt tcagaccgta
     2041 ttaagtacat cagaaccatt atcgccgagc ttaaccgaat tgcctctcac ctgctctggt
     2101 ggtcgtccat gtgtatggat atgggggcgg taacccccta cttttacgca tttcgcgata
     2161 gggaaaaaat tctcgatatt tttgaggaaa ccaccggcgg caggttgctt tacagctaca
     2221 actgtccggg aggtctaatg aacgatattc atcccaactt ccagaaaaga gttaaggagt
     2281 ttattcccta cttcaggaaa agtttcaagg agtacgacga gctggttacc gggaacgtta
     2341 tttttcagaa tcgaactatg ggtatagggc ttctgaccag ggagcaggct attgcctatg
     2401 gtgttacagg cccagctggc cgtggttcgg gatttgcctg tgatgtgagg aagagagctc
     2461 cgtacagcgc ttacgacaag gtgcagttta acgagatcct atacaacgag ggcgatag
//
LOCUS       DAOOQX010000269         2512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_50513_length_2512_cov_2.781441, whole genome shotgun sequence.
ACCESSION   DAOOQX010000269 DAOOQX010000000
VERSION     DAOOQX010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50513_length_2512_cov_2.781441"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLX53_05215"
     CDS             <1..389
                     /locus_tag="PLX53_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72515.1"
                     /translation="NKYFVIVDGSPGIGCPVVSSLSGASYVVLVTEPTVSGLHDLERI
                     YSVIKRFRIKAGCIINKYDINPKKSEEIIEFLNRENIVHLVSIPYDIAFTEAMTQAKT
                     IVETSSRISQKLIDAWNKIKEEAAKN"
     gene            395..733
                     /locus_tag="PLX53_05220"
     CDS             395..733
                     /locus_tag="PLX53_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOY72516.1"
                     /translation="MRIAFTSTGKSWDSIIDSRFGRTEYIVIFDEETKTLELVDNSAV
                     KNEAHGAGTATAQKMYEVKPDVLITGNGPGETAATALKRLNMKIYVDAHNLTLEQAYE
                     SYRNGALKEI"
     gene            751..1692
                     /locus_tag="PLX53_05225"
     CDS             751..1692
                     /locus_tag="PLX53_05225"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY72517.1"
                     /translation="MDELVFGPVPSRRLGKSIGVNNIPHKVCSYSCSYCQVGKGEKIQ
                     IERQEFYNPDTLVKQVEQKLNMTSHAEYPDYITVVPDGEPTLDINLGKLITKLKTFGL
                     PVAVITNSSLIYLDDVKRDLSNADYISFKVDAVNHSIWKYINKPHRKLDLHEILNSLM
                     NFSKYYRGKYVTETMLVKGDNTADIELAATAQFLQQLKPSIAYLSIPTRPPAFENTFP
                     PCEETLLKAYEVFYWHCLNVEYLTGYEGNAFTSSGNFENDLLGITAVHPMRIDAVKEL
                     MAKTKTNDGVLEKLVEENKVKRILYERQTFIVRSFNK"
     gene            1714..2403
                     /locus_tag="PLX53_05230"
     CDS             1714..2403
                     /locus_tag="PLX53_05230"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOY72518.1"
                     /translation="MIKGIQKSKLGIITDIILLVFLALMAGIGSLMKYVLLPGIQRNS
                     KYGADINLEFLGMDRHQWGTIHLITSKIFITLIILHIILHWDMIICILDKMIQRKLVR
                     SWFLAILAIITLTQFINPFLASPALVSYENNYRNTAERVESEPNGMDPLHKTANVEAK
                     KPIPDQSKTSRHSRAEEEYGVHGIQTINGVSVQFTIPAGFIGKELKISEGNQYQPLVV
                     VEKGMDLLCRR"
BASE COUNT          857 a          520 c          519 g          616 t
ORIGIN      
        1 aaaataaata cttcgttatt gttgatggat cgcccggaat tggctgtcca gtggtgtcgt
       61 cgttatcagg cgcaagctac gtggtgctgg ttactgagcc taccgtttcc ggattgcacg
      121 atttggagcg gatatacagt gttataaagc ggttccgtat aaaagcagga tgcataatca
      181 acaaatacga catcaaccca aaaaaatctg aagaaattat agagtttctc aatagggaaa
      241 acattgtcca cctcgtcagc attccatacg acattgcatt taccgaggct atgacacaag
      301 ccaaaacaat agtggaaaca agcagccgca taagccaaaa gcttatagat gcttggaaca
      361 aaataaaaga agaagcagct aaaaactaag taccatgaga attgcattta catcaacagg
      421 gaaaagctgg gattctatca ttgattcccg ctttgggaga acagagtaca tcgtaatctt
      481 cgacgaggaa acaaaaacgc ttgagctagt tgacaatagc gctgtaaaga acgaagccca
      541 tggagccggt acagccactg cccaaaaaat gtacgaggta aaacccgatg tccttattac
      601 aggtaatggc cctggcgaaa ctgctgcaac cgcactaaaa cgccttaata tgaaaatata
      661 tgtagatgcc cataacctaa cactggaaca agcttacgaa agctatagga atggtgcatt
      721 gaaggaaatc taggataaaa attcaaaaca atggacgagt tagtatttgg ccccgttcct
      781 tcacgtaggt tagggaaaag cattggagta aacaatatcc ctcataaggt ttgctcctac
      841 tcatgctcgt actgccaggt tggcaaaggg gaaaaaattc agatagagcg tcaggagttt
      901 tacaatccgg acactcttgt aaagcaggtg gaacaaaaac tcaacatgac cagccatgcc
      961 gagtaccccg actacataac agttgtgccc gacggagagc ctacgctgga tattaacctt
     1021 ggcaaactaa tcaccaaatt aaaaactttt gggttgcctg ttgctgttat taccaactcc
     1081 tcccttattt atttggatga tgtaaaaaga gatttatcga acgccgatta catatcattt
     1141 aaggttgatg cagtaaatca cagcatctgg aagtatatta ataaaccaca ccgtaagcta
     1201 gatctacacg aaatactaaa ctccttaatg aatttttcta aatactatag ggggaagtat
     1261 gttacagaaa ccatgctggt aaaaggagat aacactgccg atatagagtt ggcagccacc
     1321 gcacaattct tgcaacaact taaaccgagt attgcatacc tttcaattcc cactcgacca
     1381 ccggcttttg aaaatacatt ccctccttgc gaagaaactc tacttaaagc ctacgaagtt
     1441 ttctactggc attgcctgaa tgttgagtac cttaccggat acgaggggaa cgcgtttacc
     1501 tcgtcgggaa acttcgagaa cgacctgttg ggtattactg cggttcaccc catgcggatt
     1561 gatgcagtta aagagctgat ggctaaaact aaaacaaacg atggtgttct tgaaaagctg
     1621 gtagaagaaa ataaagttaa aaggatactc tacgaaaggc aaacttttat tgttcgtagc
     1681 ttcaataagt agatttcaaa aaatataaac gccatgataa aaggaataca aaagtcaaag
     1741 cttggtatca taaccgacat tatacttttg gtatttctag ctctaatggc aggcattggt
     1801 tccctcatga agtatgtttt gcttcctggc attcagcgaa acagcaagta cggagcagat
     1861 atcaaccttg aattccttgg tatggatagg catcaatggg gaactattca cctaattaca
     1921 agcaaaatct ttataaccct gattatactg cacatcatcc tgcactggga tatgattatt
     1981 tgtattcttg ataaaatgat acagcgaaaa ttagtaagaa gctggttttt agcaattttg
     2041 gcaataatta ccctaacaca atttataaac ccattcctgg caagcccggc tctggttagc
     2101 tacgagaaca actaccggaa cacagccgaa agggttgaaa gcgaaccaaa tggaatggat
     2161 ccgcttcaca aaacagcaaa cgtagaagca aagaaaccga tacccgacca aagcaaaact
     2221 tccaggcact ctagagccga ggaggagtat ggtgtacacg gcatccaaac aataaacggg
     2281 gtatcggtac aatttaccat accagcaggt tttatcggca aggaacttaa aatatcagag
     2341 ggcaaccagt atcaacctct ggttgttgta gaaaagggta tggatttact atgtcggagg
     2401 taggcaatgc cattgcaaaa atacaagaat tctctggcgg cacaataaag cacttaaacc
     2461 atgaaccaaa gtaatcaaca aaacgggtac atccaccttt atacgggaaa tg
//
LOCUS       DAOOQX010000270         2511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_50543_length_2511_cov_2.925896, whole genome shotgun sequence.
ACCESSION   DAOOQX010000270 DAOOQX010000000
VERSION     DAOOQX010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50543_length_2511_cov_2.925896"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /locus_tag="PLX53_05235"
     CDS             complement(<1..1628)
                     /locus_tag="PLX53_05235"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOY72519.1"
                     /translation="MVMVATIGMGCINAQQPDYAQWVNPLIGTDNMGHTFPGATVPFG
                     SIQLSPDTDTVPYSLDGKSYNPEVYRYCAGYQYSDKTIVGFSHTHFSGTGHSDLGDIL
                     VMPTVGQLQLNPGTSANPEKGYRSRYSHNNEYAKPGYYRVILDDYNIKAELTATTRVG
                     VHRYTFPQSDSAHIIIDMVHGIYNYPEKNVWTFVRVESDTLVTGYRVTSGWARTRTVY
                     FAIAFSKPFNTYGLVNDENLAYKGFWRKFNQNENFPENAGRRVKCHFDFTTGSNEAIM
                     LKVAISPVSTANAIENLKMEAPGWSFDNMAQHARDIWNHELGRIDVATLNQNDKVNFY
                     TSLYHAYLGPTIYMDQDGSYMGLDQNIHKATGFENYTTFSLWDTHRALHPLFNIIQPK
                     RNADMIRSMLAHYSQSVHHMLPVWSHYANENWCMIGYHAVSVIADAAVKGNTAFDLNE
                     ALNAAVATAENDWYDGVGYYMQYGYVPEDLSPNSVSKTLEYAYDDWCISQLAAKANRP
                     DLAARFYQRSRNFLNVFDKETQLMRPRLSNGSFR"
     gene            2194..>2511
                     /locus_tag="PLX53_05240"
     CDS             2194..>2511
                     /locus_tag="PLX53_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72520.1"
                     /translation="MRKKVIIGLAVVLAIILAVVLFGSSDKDKAAPQFARVMQGKFEV
                     LVNVTGELKAQNSENIMAPTELRGRTFRISEVKIQDLIAEGTLVDSGDYVASLDKSEL
                     VNQL"
BASE COUNT          626 a          518 c          658 g          709 t
ORIGIN      
        1 ttcctgaagc ttccgttgga gaggcgaggc ctcatgagtt gggtttcctt gtcaaaaaca
       61 ttcaggaagt tccgggagcg ttggtagaac ctggctgcaa ggtcgggtcg atttgctttt
      121 gctgcaagct gggagatgca ccagtcgtcg taggcgtact ccagggtttt ggagaccgag
      181 ttgggcgata ggtcctcggg aacgtaaccg tactgcatgt agtagccaac gccatcatac
      241 cagtcatttt cggcagttgc aacggctgca ttaagcgctt cgttcaagtc aaatgccgta
      301 tttcctttaa ctgcagcgtc agcaataacc gaaactgcat ggtatccaat catacaccag
      361 ttttcgttgg cgtagtgcga ccatacgggt agcatgtgat gaacgctttg gctgtagtgt
      421 gccagcattg acctaatcat gtctgcgttt cgcttgggtt gtatgatgtt gaaaaggggg
      481 tgtagcgccc tatgggtatc ccaaagcgaa aaggtggtat agttttcaaa cccggttgct
      541 ttatgaatgt tttggtcgag ccccatgtag cttccatcct gatccatgta aatggtaggt
      601 cccaggtagg catggtaaag cgaggtgtag aagtttacct tgtcgttttg gttgagtgtg
      661 gcaacatcaa ttcttcccag ctcatggttc caaatgtctc tggcatgttg agccatgtta
      721 tcgaagctcc aaccgggggc ttccatttta aggttctcaa tggcatttgc ggtgcttacc
      781 ggcgatatgg ccactttaag cataatggcc tcgttgctgc cagtagtgaa atcgaaatgg
      841 cacttcaccc gtcggcctgc attttcggga aaattctcgt tttgattaaa cttacgccag
      901 aaacccttgt aagccaagtt ttcgtcgttc accaggccgt aggtgttaaa gggtttggag
      961 aaggcgatgg caaagtaaac ggtgcgcgtt ctggcccagc ccgaagttac cctgtagcct
     1021 gtaaccagcg tatcgctttc gaccctgacg aaagtccaaa catttttttc tggatagttg
     1081 tagatgccgt ggaccatgtc gatgataatg tgggccgaat cagactgggg aaaggtgtag
     1141 cggtgaacac caacgcgtgt ggtagcagta agttcagcct tgatgttgta gtcgtcgaga
     1201 atcacccggt agtatcccgg cttggcatac tcgttgttgt ggctatatct cgaacggtag
     1261 cccttttcgg gattagcgga tgtaccgggg ttaagctgca gctggcccac cgtaggcatt
     1321 accagaatat cgcctaagtc ggagtgtccc gttccgctaa aatgggtgtg gctgaaaccc
     1381 acaattgtct tgtcggaata ctggtagccg gcgcagtagc ggtaaacctc tggattgtag
     1441 cttttaccat caagcgagta gggaacggta tcggtgtcgg ggcttagctg gatggaaccg
     1501 aatggaacgg tggccccggg aaaggtgtgc cccatattgt cggttccaat taaggggtta
     1561 acccattgtg catagtctgg ctgttgggcg tttatacagc ccattccaat tgttgctacc
     1621 ataaccattg cggaaatttt tgtaaccatt agcgtggttt ataaattgtt gtaaaggtaa
     1681 tttttttgaa gatacatgtt taccgaataa tcagcagctc gcagcagctc gcaaaggctg
     1741 ctttagccga tagaacctaa aactgttgag tggcttttgg ttgaattgat gataatcggc
     1801 cggaaatgtt gattttaaaa accatcagat atgggataat tggtaatttt attttgtttg
     1861 gctgttgatg ttgaaagtgt aaaaaaaaag ggtgattttc ccatcaaacc tgatggccaa
     1921 gcagtgcgat ggcgggttcc gagttttact cctcgacaac tttgtagtat agttttgcag
     1981 taaaaattag agcagtattt tgaaaagcct ccccatccct tttgttaggc aataaaccta
     2041 aaattttcag gacattagga gatgtgacaa tattaaaagt tgaagaggcc agtagggtag
     2101 cgcatatttt atatttttag gccagaatgt tttaccagct taaacctatt tgctattttc
     2161 aactcaaatt cttagcaaaa acagaaggtg aatatgagaa aaaaggtaat cattggcctg
     2221 gccgttgttc tggcaattat acttgcggta gttctctttg gatcgtccga taaggacaag
     2281 gcagcaccac agtttgcccg ggttatgcag ggaaaatttg aggttttggt taacgtaacc
     2341 ggcgagctga aagctcaaaa ttcagagaac atcatggccc caaccgagct gcgaggccgg
     2401 acattccgaa tctcggaggt taaaattcag gatttgatag ccgaaggaac ccttgtggac
     2461 tctggcgatt acgtggcatc actcgataaa tcggaacttg tcaatcagct t
//
LOCUS       DAOOQX010000271         2509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_50608_length_2509_cov_2.559495, whole genome shotgun sequence.
ACCESSION   DAOOQX010000271 DAOOQX010000000
VERSION     DAOOQX010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50608_length_2509_cov_2.559495"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="PLX53_05245"
     CDS             <1..169
                     /locus_tag="PLX53_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HOY72521.1"
                     /translation="RLAIPRRVYTNNHIDVVAVALKNVFDRRLEINKGLRITWEAPIM
                     RHFTVQLDRAR"
     gene            280..1638
                     /locus_tag="PLX53_05250"
     CDS             280..1638
                     /locus_tag="PLX53_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOY72522.1"
                     /translation="MRDFTTGSETQHILRFATPMLIGDIFQQLYNVVDSIIVGRFIGD
                     KALAAVGASFPVIFVIIALVLGIGIGSTVVISQYFGARNFDMVKRAANTIYIFLLISG
                     VVVTAIGLMASGAIFRMLHLPEELMKPARTYLNIFMLGMVVMFAFNAIASILRGIGDS
                     KTPLYFLLISTLTNVGLVLLFVVKFRWGIAGAAWATVISQVVAFIIALIYLNHSQHML
                     SINLRTMKFDRQIFRQIVKIGLPTGIQQTMVALGSMAMMGIVNSFGTTVIAAFTVASR
                     LDALIATPIMDFAAALSGFVGQNIGANKIDRVKRGLRSTLKISISFCVVNIAIILIFG
                     HSLMGIFTKSSDVIAIGYQYLLVVSLFYIIFAVMFSINGLLRGVGAAVVPMYITLLSL
                     WIFRIPLAYLLSKTFGMGALGIWWSIPIGWTMGAIAAVAYYRHGSWKNKSLVKVRQQA
                     AD"
     gene            2031..>2509
                     /locus_tag="PLX53_05255"
     CDS             2031..>2509
                     /locus_tag="PLX53_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72523.1"
                     /translation="MLKDYLSRIQDTINKGDAREESYYSHLDSLIKEFAIENGIKKID
                     VTILPKKTEAGNPDFRVWDGKNHITGYIEAKDPSVDNLDRIEESEQLTRYRSTFPNLI
                     LTNFYEFRLYRDGELVRKVTIGRQRIAQKLRTAPPVENEKEFNDLLGTFFSFSLPQVR
                     "
BASE COUNT          659 a          499 c          570 g          781 t
ORIGIN      
        1 caggttggca atcccccgca gggtttacac caataaccat atagacgtgg tggcggttgc
       61 ccttaagaat gttttcgacc gtagattaga gattaacaaa ggcttgcgaa taacatggga
      121 agccccaatt atgcgccatt ttaccgttca gctcgataga gcacgataat ctttttcaac
      181 tcttttgcca ttggcttgtt ctgtagtgga tcaggccaat ggttttttat ttatcattta
      241 ccccaaaaat agcctacttt tgcaattcat ttctatgcaa tgagggattt tacaaccggt
      301 agcgaaaccc aacatatttt aaggtttgca acacccatgc tgataggcga tatttttcag
      361 cagctttaca acgtggttga tagcattatt gttggtcgct ttattggcga taaggctttg
      421 gcggctgttg gagcatcgtt tccggtaatc tttgttatta ttgcccttgt gcttggtata
      481 ggaattggtt ctaccgttgt catttcacaa tattttggtg cccgtaactt cgatatggtg
      541 aagcgtgcag ctaacaccat ctatattttt cttctgattt cgggcgtggt ggttaccgcc
      601 attgggctga tggcaagcgg agccattttt aggatgcttc acctgcctga agaactcatg
      661 aagccagcta ggacctacct caacattttt atgctgggca tggtggttat gtttgccttc
      721 aacgctatag ccagcattct taggggaatt ggcgattcta aaactccttt gtattttctt
      781 cttatttcaa cgcttaccaa tgttggattg gtgctacttt ttgtggtaaa atttcgatgg
      841 ggcatagccg gtgctgcatg ggcaactgtc atttctcagg ttgtggcgtt tattatagcc
      901 ttaatctact taaaccacag ccagcacatg cttagcataa acctacgaac catgaagttc
      961 gaccgccaaa ttttcaggca gattgttaaa attgggttac ccaccggaat acagcaaact
     1021 atggtagccc ttggttcaat ggcgatgatg ggaattgtta attcttttgg aaccaccgtt
     1081 attgcggctt ttactgttgc tagccgtttg gatgcactaa ttgcaacccc tataatggat
     1141 tttgccgccg ctctttcggg atttgtgggg caaaacattg gtgccaacaa gattgacagg
     1201 gtaaaaaggg ggctacggtc aaccctgaaa atttccatta gcttttgtgt tgtcaacatt
     1261 gccattattc tgatttttgg ccacagcctg atggggatat tcactaaaag ttccgatgtg
     1321 attgccatag gttaccagta cctgctggtt gttagcttgt tctacataat ttttgccgta
     1381 atgttttcca tcaacgggtt gctcagaggg gtgggcgctg ctgttgttcc catgtacatc
     1441 accctgctgt cactctggat ttttcgaatc cctcttgcct acctgttgag caaaaccttt
     1501 ggtatgggtg cattgggtat ctggtggtct attcctattg gttggacgat gggggccatt
     1561 gctgcagtag cctactatcg ccatggaagc tggaagaata aatccctggt aaaggtacgc
     1621 cagcaagctg cggattagag ttgagatggt agcaagtaag tagctccgaa aaccaaaagg
     1681 ttgaatatct caccgaaaca aaactatttg ccatttttgc ttggattgaa ttccattttt
     1741 tatcctaaag ctggttaata aattagatgt tgatggctgt ttcctaaaaa ttttatattt
     1801 gtaggagcaa tataatggca ttccattaaa tactcattaa gctacactgg ttgataatga
     1861 tggtaattca tgtcaggcaa catgatttca taaacgggtg atggagttct tgagttctat
     1921 aaaactattg tgggtgcttt tcaatctggg caggtacttt tccttgcgtc aatagttttt
     1981 atttattggt ttgctgccgg gtaaattcta tcatataaac tttttaaaac atgctaaagg
     2041 actatttaag cagaattcaa gataccatta acaagggaga tgccagggag gagagctact
     2101 actcacatct agatagcctg attaaagaat ttgcaataga aaatggcatt aaaaagattg
     2161 atgtaacaat acttcccaag aaaacagaag cggggaatcc cgatttccga gtatgggacg
     2221 ggaagaacca tataaccggg tatattgagg caaaggatcc ttcggttgac aatcttgata
     2281 gaattgagga atcggagcag ctaacacgat accggagcac ctttcccaac ttaattctga
     2341 cgaattttta cgagtttagg ctatacaggg atggcgaact ggtaagaaag gttacgattg
     2401 gccgacagag gattgcccaa aagttgagaa cagctccacc ggtggagaac gagaaagaat
     2461 tcaacgacct gcttggcacc ttcttctcct tttccctacc acaggtacg
//
LOCUS       DAOOQX010000272         2497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_50928_length_2497_cov_2.546274, whole genome shotgun sequence.
ACCESSION   DAOOQX010000272 DAOOQX010000000
VERSION     DAOOQX010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50928_length_2497_cov_2.546274"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..833)
                     /locus_tag="PLX53_05260"
     CDS             complement(75..833)
                     /locus_tag="PLX53_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOY72524.1"
                     /translation="MKKSFIVVGIILFLCLPGHSQVWKRNRIEIFLGVPITHYFGDIG
                     GTSDPSSMMGIKDISIRAIRPGFSGGLGYRLTERFYAQGTLTLGLFGNSDKGSRNEAR
                     NYAFSTFGTELAAVAQFYIIPESDQNYFYSIMQVRGGLRKINKPFSLYLFAGGGGCFY
                     RVKPLNDLVDSDRFDDSQHTAVVVPFGLGFKYQIHSRMLLGVELGGRYVLSDYLDGFS
                     PEPSKYNDFYYVFNFKVHYRLPSNKILRKPQWTF"
     gene            complement(917..1795)
                     /locus_tag="PLX53_05265"
     CDS             complement(917..1795)
                     /locus_tag="PLX53_05265"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOY72525.1"
                     /translation="MVVAIYGKAVETEYLPSLGELFNQLVEHNVEVWVYRPFMEYLLE
                     TFSAKLPDFEVFNTGSDINGKANILISIGGDGTFLDSVSFVRSSGLPIMGINFGRLGF
                     LANIAIPDMHNAIDLLIAGRFKVEHRSMLEVVSTLEQFNTFPFGLNDFTMQKASSGFL
                     KVNAYVNGEYLCAYWADGLIVSTPTGSTAYSLSVGGPIVSPNLSALILSAIAPHNLTV
                     RPLVISDDNILLLEAVGRDGSVMLSLDSRSITCKSPVTVTVKKAPFFANVVSMEGSNF
                     YHTLRGKLLWGMDKRN"
     gene            complement(1814..>2497)
                     /locus_tag="PLX53_05270"
     CDS             complement(1814..>2497)
                     /locus_tag="PLX53_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOY72526.1"
                     /translation="FFKPPTMIALEMISDVIPALKTSDTGQTALNWMDIFKVSHLPIV
                     NNKEFLGLVSESDIYDLNMPDEPLGNHYLSLNSPYVIEDQHIFEVMEVLSRLKLSLVP
                     VLDSGKNYLGVITLMELLHYFAVESGISLQGGIVVLEMNANDFSMSQVCQIVEGNDAK
                     ILAAYVTPHPNSTRMELTLKLNVTDLTSIRQTFNRYNYTVKASFLKQDDEADMLNDRI
                     NFLFRYLDI"
BASE COUNT          769 a          572 c          517 g          639 t
ORIGIN      
        1 agatttgaag ggcaacaaca aaaaaaatga aagaacaagt aaacttgaca aaaacccgaa
       61 aaatatcctg ttagctagaa tgtccattgg ggctttctta aaattttatt actgggaagc
      121 ctgtagtgca ctttgaagtt aaaaacgtag tagaagtcat tatattttga gggttctggt
      181 gaaaatccgt caaggtagtc agatagcaca tatcgtcccc ccagctcaac tccaagcaac
      241 attctggaat gaatttgata tttaaacccc agaccaaaag gtactacgac tgctgtatgc
      301 tgtgaatcgt caaatctatc ggaatcaaca aggtcattaa ggggtttaac cctataaaaa
      361 catccaccac cgccggcaaa aaggtacagg ctaaaaggtt tgttaatttt tcttaaaccc
      421 ccgcgaacct gcatgatgct gtagaagtag ttctggtcgc tctcgggtat aatgtagaac
      481 tgcgcaactg ccgctaattc ggttccaaaa gtagaaaagg cgtagttgcg tgcttcattc
      541 ctagagccct tgtccgagtt tccaaaaagg cctagagtta aagtaccctg ggcataaaaa
      601 cgctcagtta gcctgtaacc caaccctccc gagaaaccag gtcgtattgc cctgatgctg
      661 atatccttta tccccatcat actgctagga tcgctggtcc caccaatatc tccaaagtaa
      721 tgggttatgg gaactcccaa aaatatttca attctatttc gtttccatac ctgggagtgg
      781 ccaggcagac aaagaaaaag aattatccct accactataa aggatttctt cattagccag
      841 tattatcaac tacaaatata taacaattaa ctatttaaaa attccattct atgcaatcca
      901 acagctttag ctgccattag tttcgtttat ccattcccca aagcaacttt cccctaaggg
      961 tatgatagaa gttcgatccc tccatgctga caacattggc aaaaaaagga gctttcttaa
     1021 cggtaacggt aaccggcgac ttacatgtta tgcttcggga atccagcgat agcataacac
     1081 tgccatcacg tccaacagcc tcgagcagta ggatgttgtc atcgctgatt accaacggac
     1141 gcacagtaag gttatgaggt gcaatagccg acaggattag ggcagaaagg tttgggctaa
     1201 caatgggtcc gcctacgcta agcgagtagg ccgtggaacc ggttggagta gagacaataa
     1261 gcccgtcagc ccagtaggca caaaggtact ccccatttac gtaagcgttc accttaagaa
     1321 atcccgaact agctttctgc atggtaaaat cattcagccc aaatggaaaa gtgttaaatt
     1381 gctccagcgt tgaaacaact tccagcatgc ttctatgttc aactttaaac ctccctgcaa
     1441 tcagcaaatc aatggcattg tgcatgtcgg gaatggcaat gttggccaga aaacctaacc
     1501 taccaaagtt aatgcccata ataggcaagc cagatgaccg gacaaacgaa acagaatcaa
     1561 gaaacgtgcc atcaccccca atgctgataa gtatgttagc cttcccgttt atatcgctac
     1621 cggtattaaa aacttcaaag tcaggcagct tggccgagaa agtttctagg agatactcca
     1681 tgaagggtct gtaaacccaa acctctacat tatgctcaac cagctggtta aaaagttcac
     1741 ccaaggaggg aagatactcc gtttcaaccg cttttccata aattgcaacc accatagggt
     1801 cgaattgttt agtttatatg tcgagatagc gaaaaagaaa attaatccta tcgttcagca
     1861 tgtcggcctc atcatcctgc ttaagaaacg aagctttaac ggtgtagttg tacctgttga
     1921 acgtttgcct gatagacgtc aaatcggtaa cattcaactt tagggtaagc tccatcctgg
     1981 tggagttggg gtgcggggta acgtaggcgg caagaatttt tgcatcgttt ccctcaacaa
     2041 tttggcaaac ctgagacatg gagaaatcgt tggcattcat ttccagcacc actatgcccc
     2101 cctgcaggct tattcccgat tctacggcaa agtagtgtag cagctccatc agggtaatca
     2161 cgccaaggta gttcttaccg ctatctaaaa cgggcaccag cgatagcttc agacgcgaaa
     2221 gcacttccat aacttcaaat atgtgctggt cctcaataac ataagggcta tttagcgata
     2281 ggtaatggtt acctaggggt tcatcgggca tgttcaaatc gtaaatgtcc gattctgaaa
     2341 ccaaccctaa aaactccttg ttgttaacaa tgggtaggtg tgaaactttg aaaatatcca
     2401 tccagtttag agctgtttgg cccgtatccg aagttttaag ggcaggaata acatccgaaa
     2461 tcatctccag agctatcatg gtaggcggct tgaaaaa
//
LOCUS       DAOOQX010000273         2495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_50976_length_2495_cov_3.586885, whole genome shotgun sequence.
ACCESSION   DAOOQX010000273 DAOOQX010000000
VERSION     DAOOQX010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50976_length_2495_cov_3.586885"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2458
                     /gene="uvrA"
                     /locus_tag="PLX53_05275"
     CDS             <1..2458
                     /gene="uvrA"
                     /locus_tag="PLX53_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOY72527.1"
                     /translation="LFARIGRTYSPVSGKEVKKHSVSDVVNFISSFNEGTKMMLLAPL
                     TPNGDMKQKLEALAHEGFTRVEVGNQIVRIDEQKFREVVDLSGSELRLVIDRFAAAAD
                     DEFLSRLADSVQTAFAEGKGFCLLKVFDDNGSYSLEQFSSLFEMDGIKFEEPTEHLFS
                     FNSPLGACPTCEGYGKIVGIDENLVIPNRSLSVYDDAIACWKGESMSWYKDRVVENAS
                     RYGIPIHKPYFQLSEEQKSLLWEGTPHFTGINDFFRELEGKKYKIQYRVLLSRFMGKT
                     TCPTCRGGRLRREATFVKVNGYTIQELVEMPVADLAEVFNNLELTEHEVGVAKRILIE
                     LKNRLNYLVNVGLGYLTLNRLSSSLSGGESQRINLATSLGSSLVGSLYILDEPSIGLH
                     PRDTHLLIDVLKKLRNMGNTVVVVEHDEEIIRASDYIVDIGPYAGVNGGEVVFEGPIE
                     NLEKATQSLTANFLLSREQILVPRVRRKWNSYIEISGARENNLKGIDVKFPLSCLVVV
                     TGVSGSGKSSLVDDILYPALLKQLTGAGPRAGQFDSLGGDWKLISGVELVDQNPIGRS
                     SRSNPVTYIKAWDEIRKLLSEQPYAKRNGYKPAHFSFNVEGGRCEECQGEGVIHVEMQ
                     FMADLTLVCESCKGKRFKDDILEVRYNGANVYDILNMTVDQAIEFFSKGKTLTEQKVV
                     DRLKPLQDVGLGYIQLGQSSSTLSGGESQRVKLASFLQKDASIRPLLFIFDEPTTGLH
                     FNDIRQLLNAFDALLAKGHSLVIVEHNLEVIKSADWVVDLGPEGGQEGGLLVFAGTPE
                     DLAANEASHTGVFLKKKLNG"
BASE COUNT          644 a          527 c          679 g          645 t
ORIGIN      
        1 gctctttgcc cgaattggaa gaacatactc ccccgtatcg gggaaagagg tgaaaaagca
       61 ttctgtcagc gatgtggtta acttcatcag ctcctttaac gagggaacca aaatgatgct
      121 tttagcaccc cttacaccga atggggatat gaagcagaag ctggaagctc ttgctcacga
      181 aggctttaca cgtgttgagg ttggaaatca gatagttcgg attgatgagc aaaaattcag
      241 ggaggtggtt gacctttcgg gatcggagtt gcggctggtt atcgatcgtt ttgccgcagc
      301 cgctgatgat gaattcctta gccggttggc cgactcggtt cagactgcct ttgcagaggg
      361 gaaaggcttt tgcctgttga aagtattcga cgacaatggg agctatagcc tggagcaatt
      421 ctccagcctc ttcgagatgg acgggattaa gtttgaagaa cccaccgagc acctgttcag
      481 ttttaatagc cctttgggtg cttgccctac ttgtgagggc tatgggaaaa ttgtaggaat
      541 cgatgaaaat ctggtcatcc ctaaccgtag tctttctgtt tacgatgatg caattgcctg
      601 ctggaagggt gaaagtatga gctggtataa ggatagggtt gtggaaaatg ccagccgata
      661 cggaatacca attcataaac catactttca gctttccgag gagcagaaat cgctactttg
      721 ggaaggaact cctcatttca ctggaattaa tgactttttc agagagctgg agggaaaaaa
      781 gtataagata cagtatcggg ttttgctttc ccggttcatg ggaaaaacta cctgcccaac
      841 ctgcagggga ggaagacttc gtcgtgaggc aacttttgtc aaggtgaacg gctataccat
      901 acaggaattg gttgagatgc cagtagccga cttggcggaa gttttcaaca atctcgagct
      961 caccgaacat gaggttgggg tggccaaacg aattcttatc gagctaaaaa atcgcttgaa
     1021 ctacctggtc aacgtggggc ttggatacct cacgctcaac cggctctcct catcactgtc
     1081 gggaggtgaa tcgcagcgta tcaacctggc cacatctttg ggaagcagcc tggtaggttc
     1141 gctgtatatc ctcgatgaac ccagcatcgg gctacatcct cgtgacaccc acctgctcat
     1201 tgatgttctg aagaaactgc gtaacatggg caatacggtg gttgttgttg agcacgatga
     1261 ggaaatcatc agggcttccg attatatcgt tgacattggc ccctatgccg gggttaacgg
     1321 aggcgaggtt gttttcgaag gacccatcga aaaccttgaa aaagccactc aaagcctaac
     1381 ggccaacttt cttttaagca gggagcaaat actcgttccc agggttcgga gaaaatggaa
     1441 tagctatatt gagatttccg gagcccggga aaataaccta aagggtattg atgttaaatt
     1501 tccgcttagc tgtctggttg tagttacagg tgtaagcgga tcgggtaaat cgagcctggt
     1561 ggacgatatt ttataccctg cccttctgaa gcagctaacg ggtgctggtc cccgtgccgg
     1621 acagtttgat tcgctaggtg gcgactggaa gctaatttca ggggttgagc tggtagatca
     1681 gaatccaatt ggccgatcgt cgcggagcaa tccggttacc tacatcaagg catgggatga
     1741 gattcgtaag cttttatccg aacaacccta cgcaaaacga aatgggtata agcccgctca
     1801 tttctccttt aatgtcgagg gtggacgatg cgaggagtgc cagggtgaag gtgttattca
     1861 tgttgagatg cagtttatgg ccgatttgac cttggtttgc gagagctgta aggggaaacg
     1921 ttttaaggat gatatcctgg aggtgaggta caacggagcc aacgtttatg acattttaaa
     1981 catgacggtt gatcaggcca ttgaattttt tagcaagggg aaaactttaa ctgagcagaa
     2041 agttgtggat aggctaaaac cgcttcagga tgttgggctg ggctacatac agctgggaca
     2101 atcgtccagc accctcagcg gtggggaaag ccagcgggtg aaacttgcct ctttccttca
     2161 aaaggatgcg tccattcgcc cgcttctttt tatttttgac gaaccaacca ctggtcttca
     2221 tttcaacgat attcgccagt tgctgaatgc ctttgacgcc ctgctggcta aaggacacag
     2281 cctggtcata gttgaacata accttgaagt gataaaaagt gccgactggg tagtagattt
     2341 gggcccggaa ggcgggcagg agggaggttt gctggttttt gccgggactc ccgaagatct
     2401 tgctgccaac gaggcgtccc atacgggtgt atttctaaaa aagaagctga atggttaata
     2461 aaaaaagccg catgtagcgg ctttgtacag tctta
//
LOCUS       DAOOQX010000274         2484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_51310_length_2484_cov_2.560313, whole genome shotgun sequence.
ACCESSION   DAOOQX010000274 DAOOQX010000000
VERSION     DAOOQX010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51310_length_2484_cov_2.560313"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PLX53_05280"
     CDS             <1..1085
                     /locus_tag="PLX53_05280"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY72528.1"
                     /translation="SRQLNILVCPLGWGLGHASRVVPIVRLLLESGHRVVVAADANSL
                     ALVKSSCPYLETKFFPSYKVQFKRGSSQLLPMLWVAIRLPFINLREHRKLKKLVLENH
                     IDLVISDNRYGLWLRGIMSVMVTHQLRVIPPRPFKFLTKASEILIRHWLKRFNHVWIP
                     DNEGAGSIAGVLSSPNGLTNIQYIGILSRFSGIAPASNFKTWEMVVMVSGPEPQRALL
                     VELAANLAHRYSIGCLVVEGKPQNGSELRVVDGIWYAGHLPDEQLAFVLRSCRYLLVR
                     GGYSTIMDLVTLGISGLLIPTPGQTEQEYLAHYLSVKGLFRSVPQQCLPQLDLKEAER
                     RSIPLHSEYSGSPRLKSCVDGVVFRL"
     gene            complement(1184..1981)
                     /locus_tag="PLX53_05285"
     CDS             complement(1184..1981)
                     /locus_tag="PLX53_05285"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOY72529.1"
                     /translation="MSATLSFGQPFGVLTYNIRLDTEQDGVNRWDNRKESMVELVNRY
                     SPAIFGVQEALYHQVCYLDSALKKYTYIGVGRDDGKKAGEFSAIFFDSTRLRVIAHST
                     FWLSEHPDSVSLGWDAACRRVCTYGLFEDMLSHKKFWVFNTHFDHVGKVARKNSAELI
                     VNRINELNGDNLPVIAMGDFNSNPADEPVKILASTLADGLTCSQRPLNGPVGTFNGFN
                     PEYLPDERIDYIFVLKVRVLTYSHLTDRRENGLLISDHLPVLAEVEI"
     gene            complement(2170..2337)
                     /locus_tag="PLX53_05290"
     CDS             complement(2170..2337)
                     /locus_tag="PLX53_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOY72530.1"
                     /translation="MAYKISDDCTACGTCIDECPVEAISEGDIYKINPDICTDCGSCA
                     DVCPVEAIHPE"
BASE COUNT          657 a          564 c          582 g          681 t
ORIGIN      
        1 cctctcggca gctgaacatt ttagtatgcc ctcttggatg ggggctcggc catgcaagca
       61 gggttgtccc tattgttcgc ttactgctgg aaagcggaca tcgggtggtg gttgcagccg
      121 atgcgaattc gctggctctg gtaaaaagct catgccctta cttagaaacc aaattttttc
      181 cgtcgtacaa ggtacaattt aagcggggct ccagccagct tctgcctatg ctttgggttg
      241 ccatcaggct tcccttcatc aacctaaggg aacatagaaa gctgaaaaag ctggttttgg
      301 aaaaccacat tgacctggtg atatccgaca accgctacgg gttgtggctg cgtggaatta
      361 tgtcggtgat ggtaacccac cagcttaggg taattccgcc cagacctttc aaattcctta
      421 ctaaagcgtc ggaaatacta ataagacatt ggttgaaacg cttcaaccat gtttggattc
      481 ccgataatga gggtgctggc agtattgccg gagtgctgtc cagccctaac ggattgacca
      541 acattcaata catcggaata ctatcacgtt tttcagggat agcgccagca tccaatttta
      601 aaacctggga aatggtagtt atggtttcgg gtcccgagcc ccagcgtgcc cttctggttg
      661 agctggcagc aaacctggcc caccgttact ccattggttg cctggtggtg gaggggaaac
      721 ctcaaaatgg tagtgaactt agagtggttg acggaatatg gtatgccggt catttacccg
      781 acgagcagct ggcattcgtg ttacgtagct gtaggtacct gctggtcagg ggtggttata
      841 gcaccataat ggatcttgtt accctaggca tttcaggatt gcttattccc actccaggcc
      901 aaaccgagca ggaatacctg gcgcactacc tgtcggtaaa gggtctattc cggtcggttc
      961 cgcagcaatg cctgcctcaa ctcgacttga aagaagcaga acgacggagc atacccctgc
     1021 attcagaata ctctggtagc ccaaggttaa agagctgcgt tgatggggtt gttttcagac
     1081 tctagaatca cttttgccaa aaaaaaataa taggccgaaa caatgccatg atttaggcta
     1141 agacaactct agtaaattca ataaaatctt aaattgaagg tattcatatt tccacctcag
     1201 caagaacagg aagatggtcg gagattaaaa gaccattttc ccttcggtcg gtaagatggc
     1261 tgtaggtgag cactcgcact ttcagaacaa atatatagtc aatcctttca tcaggtaggt
     1321 attcagggtt aaacccatta aaggtaccaa ccggtccatt caacggcctt tgagagcaag
     1381 ttaacccatc ggcaagcgtt gaggctaata tcttcaccgg ttcgtcagcc ggattgctgt
     1441 tgaagtcacc cattgctatt accggcaaat tatcgccatt cagctcattt atcctgttga
     1501 ctatcaactc tgcggaattt ttacgggcaa ccttacccac atggtcgaag tgggtgttga
     1561 aaacccaaaa ttttttatgg gaaagcatat cctcaaaaag cccgtatgtg caaactcttc
     1621 tgcaggctgc gtcccagcct aatgaaacgc tatcggggtg ctccgatagc cagaaggtgg
     1681 agtgggcaat caccctaagc ctggtgctgt cgaagaaaat agcgctgaat tcgccggcct
     1741 tcttcccatc gtctctgcca acgccaatgt aggtgtattt ttttaaggca ctatcaaggt
     1801 agcagacctg atggtacagc gcttcctgaa ctccaaatat ggcggggctg taacggttga
     1861 ctagctcgac cattgattct ttcctattat cccatcgatt taccccatcc tgctcggtat
     1921 cgaggcgaat gttgtaggtt agcacgccga atggttgacc aaagcttagc gtggcgctca
     1981 ttgcaagtac tgccagtatt aaaggttttc gaaaaggttg tgtggtcctt tttttgattt
     2041 gcattgttga agcggtttta gctcaaagga acatggtttg ttcagttcgt catgctacca
     2101 atcagggggt attcatatat aaaacaaaat ccgcatgatt tgcggatttt gataaaatta
     2161 ctgctgtaat tactcgggat gaatggcctc taccgggcaa acatcggcac atgaaccaca
     2221 gtcggtgcaa atatcggggt taatcttata gatatcgccc tcggatattg cctcaacagg
     2281 acattcatca atgcaagtac cacaagcggt gcaatcgtcg gaaattttgt atgccatttc
     2341 tgcaagtttt agttaaactg aattcatcac aaaaatagct ttttaatgta ttaccaaaaa
     2401 atatgatgtg aaattttaat taattcttgt aggagagatt tcgagattca cagtcgagta
     2461 tcactaaacg gcgatattcc cctt
//
LOCUS       DAOOQX010000275         2470 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_51749_length_2470_cov_2.595859, whole genome shotgun sequence.
ACCESSION   DAOOQX010000275 DAOOQX010000000
VERSION     DAOOQX010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51749_length_2470_cov_2.595859"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="PLX53_05295"
     CDS             complement(<1..133)
                     /locus_tag="PLX53_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOY72531.1"
                     /translation="MNIIIVGAGEVGTHLATMLAKEYHDVTVIDPDPEMLSHVTAVSD
                     "
     gene            complement(222..2330)
                     /locus_tag="PLX53_05300"
     CDS             complement(222..2330)
                     /locus_tag="PLX53_05300"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOY72532.1"
                     /translation="MSLPSSYHEVPFFRLLVPLVVGILVQLSLQPLHASMNILLLPVI
                     GFCILVVLSLLRKWELRWVYGLMLNLFMLVCGLVLSVNFTFNDDLSDLHEQGVVVRLT
                     ESPQLRARSLRAVGKVVRVMDKGSISPNEEKVLLYFSLTDSAAAQLQCGDIMAALLRF
                     STIEEPQNPYQFNFKQYMLARQIRFSSFVEPDSWARVGHHENGLVQLAGDLRRKLLAM
                     FSEFGLKDREYAVVSALTVGYTDLLDDALQKTYSTTGAMHILSVSGLHVGLVYGLLAF
                     LLSFLPKRAFFRIIKLPIQVVFLWFFALITGLSPPVCRSAFMFSLVAVGQGFNFRTNI
                     LNTLAAAAFALLVANPNNLLQVGFQLSFLAVLSIVVFHPFIYRLFTFKSKILDYFWSL
                     IAVSVAAQLGTIPVTLAAFGQFPTYFMLSNIIAIPLSTLILYAAVALVVFSPVPLVGA
                     FIGKILLVLTRSLNGGLQWIESLPHSSISGVYISRLQMLVLLSALVFISLYIVLKHSS
                     QLRAALLLMLFFFMLNIPQLRPVIRNELVVFHLPGSSMLCIRNNGLPIFIGTDSVGRN
                     LVEDSDFFLGGYISRQPSGRYGYINLCSPDWELLRQNGISLQRKYGLAVLEYNGRTVA
                     LPYSDSLRYATFGQTLNADVLVVNRYARGNLPDFIKPEVTVVDASVRFKHASAMLTRF
                     TGRGSAIHNLSVDGAYTAAL"
BASE COUNT          773 a          604 c          597 g          496 t
ORIGIN      
        1 catcggaaac agcggtaaca tggctgagca tttcaggatc gggatcaatc accgttacat
       61 cgtgatactc cttggccagc atggtagcaa gatgcgttcc tacctcacct gccccaacaa
      121 tgattatatt catggcaccc taaaaagagg ttttagtgca tattttacag ctgcaaagta
      181 atactaaaaa cagcaaaccc attgccaggt tttcaataat tttagagggc agctgtatag
      241 gctccgtcaa ccgacaggtt gtggatggcc gaccccctgc cggtaaacct ggtaagcatc
      301 gccgaagcat gtttaaacct aaccgaggca tccacaacgg taacttcggg tttgatgaaa
      361 tcgggaaggt ttccacgggc atatcggtta accaccagaa catcggcatt gagcgtttgc
      421 ccaaatgtag cataccggag cgaatcgctg tagggtaacg caaccgttcg accattatac
      481 tccagcacag ccaatccgta ttttcgctgg agcgatatgc cgttttgtcg cagtagctcc
      541 caatcggggg agcacaggtt aatgtagcca tagcgccccg agggttgccg ggagatatac
      601 cctcccagga aaaaatcgct atcctcaact aaattccgac caaccgaatc ggtaccaata
      661 aatatgggca gcccgttgtt ccgaatacat agcatagagc tgccaggaag atggaaaacc
      721 accagctcgt tccggattac aggtctaagc tggggaatat tcagcatgaa gaaaaaaagc
      781 ataagtaaaa gtgctgcacg cagctgcgat gaatgcttca gcacgatata cagcgagatg
      841 aacaccaacg ccgagagcag aacaagcatt tgcagcctgg agatgtaaac tcccgatatg
      901 gaggaatgcg gcaggctttc aatccactgc aatcccccgt taagagaacg agtaagaaca
      961 agcagaattt tgccgatgaa tgccccaacc agcggaacag gcgaaaaaac caccagggct
     1021 acagccgcat acagaattag cgtggataac ggaatggcta taatgttgga tagcatgaag
     1081 taggtgggga attgcccaaa agcagccagg gtaacgggaa ttgttcctag ctgggcagcc
     1141 actgaaacgg caatcagcga ccagaagtag tccagaattt tgcttttgaa agtaaacagc
     1201 ctgtagataa aggggtgaaa aaccacgata gagagcaccg ccagaaacga aagttgaaac
     1261 cccacctgca gcaggttgtt gggattagct acaagaaggg caaatgctgc agcggccagc
     1321 gtgtttagaa tattggtgcg aaagttaaag ccctggccaa cggcaaccag cgagaacatg
     1381 aaagccgaac ggcaaacagg cggggatagt ccggtaataa gcgcaaaaaa ccagagaaat
     1441 accacctgaa tgggtagctt aattatccgg aaaaaagccc gtttcgggag gaagctgagc
     1501 aaaaaggcca gcaggccata aaccaggcca acatgcaaac cggaaacgga aagaatgtgc
     1561 atggccccgg tggttgaata ggttttctgg agcgcatcgt cgagcaggtc ggtgtagcca
     1621 accgttaacg ctgaaacaac ggcatactct ctatccttaa gcccgaattc ggaaaacata
     1681 gccaacagct ttctacgcaa gtctcctgcc agctgaacaa gaccattttc atgatgccca
     1741 accctggccc agctgtcagg ctcaacgaag ctgctgaagc gaatctggcg ggcaagcatg
     1801 tactgcttaa agttgaattg gtaggggttt tgtggctcct ctattgtgct gaacctaaga
     1861 agggcagcca taatatcccc gcactgcagc tgagcggcag cagaatcggt taacgagaag
     1921 taaagcaaaa ccttttcctc gtttggtgaa atgctcccct tatccatcac ccgaaccact
     1981 ttcccaactg cccgcagaga acgggcacgt agctggggac tttccgtcaa ccttactact
     2041 actccttgct catgtaaatc ggaaaggtca tcattgaagg taaagttaac ggaaagcaca
     2101 agcccgcaga ccagcatgaa aaggttaagc attaggccat aaacccagcg cagctcccat
     2161 tttctcagca gtgatagcac caccagaatg cagaacccta tcaccggtaa aagaagaatg
     2221 ttcatgctgg catgaagcgg ttgcaggctc agctgaacga gaatccccac aaccagcggt
     2281 acaagcagcc tgaaaaatgg cacctcgtgg tagctggaag gtaacgacac tgcagtagca
     2341 attaaactta taaacaagtt aatagaattc cactgcttgt caaaaaaaaa attaatccaa
     2401 caggctgtta actccacaag agcaaaaaat gatttaacgg aaaaatgcag ctactaccat
     2461 tttaatccct
//
LOCUS       DAOOQX010000276         2459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_52049_length_2459_cov_3.821547, whole genome shotgun sequence.
ACCESSION   DAOOQX010000276 DAOOQX010000000
VERSION     DAOOQX010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52049_length_2459_cov_3.821547"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="PLX53_05305"
     CDS             complement(<1..1137)
                     /locus_tag="PLX53_05305"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOY72533.1"
                     /translation="MYGKIQKDLQKQLEAIVNDGLYKTERAISTPQGAEIKANGKKVL
                     NFCANNYLGLSSHPAVLEAGKKAIDEWGFGMSSVRFICGTLELHKQLEAKLTDFLGTE
                     DTILYSSAFDANGGVFEPILGEEDAIISDELNHASIIDGVRLCKAQRFRYKNNDMDDL
                     ERVLKEASGCRYKMIVTDGVFSMDGIIAQVDKICDLADKHDALVMVDDSHATGFVGKT
                     GRGTSEYCNVMGRVDVISTTFGKALGGASGGCISGRKEIVDMLRQRSRPYLFSNSLMP
                     AIAGSTLMVLNMLSKSTELRDKLWENTALFRKGMTDAGFRIVPGVHPITPILLGHLPN
                     DAKLSQDFADMLFDEGIYVTGFFYPVVPKGKARIRVQLSAAHSRE"
     gene            1373..2107
                     /locus_tag="PLX53_05310"
     CDS             1373..2107
                     /locus_tag="PLX53_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72534.1"
                     /translation="MPYRRLPNTDSARIRALKAAYSKGKLNPPSQLAFSQSTFSKVEL
                     FINSFQNALAHYKVAYNQQVESGKEYSQILKKAKLYLSHFIQVLNMAIARGELPVSVR
                     EIYGMDLDENRMPSLTTEKELIEWGKKIIDGEMQRLQKGQTPITNPTIAVVRVRYEQF
                     LEAYNHHKILQQNTRRLLNDVNNLRVTADSIILSIWNEVEDYFKDLPDEERRLKASEY
                     GVVYVYRKNEIKDFRLEDRTLLKLFN"
     gene            complement(2170..>2459)
                     /locus_tag="PLX53_05315"
     CDS             complement(2170..>2459)
                     /locus_tag="PLX53_05315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOY72535.1"
                     /translation="FIPRFALGVIFGYLYEWSRSLWLPMLAHFTNNAIAVIAFLLMGS
                     EISVNEIEEVGSLQDIWAIGIVSFLAVALLLRFIWKTNTRQSPVSQNMVKS"
BASE COUNT          681 a          590 c          582 g          606 t
ORIGIN      
        1 ttccctactg tgggcagcac taagctgaac acgtatgcgg gcctttccct ttggaacaac
       61 cggataaaag aaccccgtaa cgtaaatgcc ctcgtcaaaa agcatatcgg caaaatcctg
      121 cgacaacttg gcatcgttgg gtaggtgtcc aagcaggatg ggggtaatgg ggtgaacacc
      181 aggtacgatt ctaaaaccgg catcggtcat accttttcgg aaaagggcgg tgttctccca
      241 aagcttatcg cgcagctcgg tgcttttgct gagcatgtta agaaccatga gtgttgatcc
      301 ggcaatggcc ggcattagcg agttggagaa aaggtagggt ctggagcgct ggcgaagcat
      361 gtcaacaatt tccttccgtc ccgaaatgca accgcccgaa gctcctccaa gagccttacc
      421 aaaagtggta gagatgacgt caaccctgcc cattacgttg cagtactcgg aagttcccct
      481 tccggttttg ccaacaaatc ctgtggcatg ggagtcgtca accataacca gcgcatcgtg
      541 cttatcggca aggtcgcaaa ttttatctac ctgggcaatt atgccgtcca tggaaaaaac
      601 gccatcggtt acgatcattt tatagcggca accggaggcc tccttcagca cgcgctccag
      661 atcgtccatg tcgttgttct tataccggaa gcgctgagcc ttgcacagcc ttaccccgtc
      721 aataatggag gcgtggttga gctcgtcgga gataatggca tcctcttccc ccaaaatggg
      781 ttcaaaaacc cctccgttgg catcgaaagc cgaagagtaa agaatggtat cctcggtgcc
      841 gaggaagtcg gtaagcttgg cttcgagctg cttgtgcagc tcaagggtgc cgcatataaa
      901 ccgaaccgac gacatgccaa aaccccattc gtctatggct ttctttccgg cttccagcac
      961 agcaggatgc gatgaaagcc caaggtagtt gttggcacaa aagttcaaaa cttttttccc
     1021 gttagcctta atttcagcgc cctgtggtgt agagattgca cgctcggttt tgtagaggcc
     1081 gtcgtttaca atagcctcga gctgcttctg taaatctttc tgaattttac cgtacatggt
     1141 ataatcattt taaattctct ttacaaaagt agaagaattt tatgcggggc gagctatgaa
     1201 atcgcctttc ttttcttcat ttctcaaaaa aatatctact aagcaaaata tttgccatgc
     1261 aatttaaaaa ttatttaatg cgaatagatg gcttttgtaa tcatgatttt ttatattcgc
     1321 aaattgtagg tagttaattc tcagagcgaa aaacccaaca accgattaaa ttatgcccta
     1381 ccgccggtta cccaacacag atagcgcaag gatacgcgct ttaaaggctg catacagcaa
     1441 gggaaaatta aatcctccat cgcagctggc cttttcgcag tcaaccttca gcaaggttga
     1501 gctattcatt aactctttcc agaatgcgct ggctcattac aaggtggctt acaaccagca
     1561 ggtggagagc ggcaaggagt actctcaaat tttgaaaaag gcaaagcttt acctttcgca
     1621 cttcatacag gttttaaaca tggcaattgc ccgaggcgag cttcctgttt cggtgaggga
     1681 gatttacggg atggatttgg acgaaaacag aatgccgagc ctgacaaccg agaaggagct
     1741 gatcgaatgg ggtaaaaaga ttattgacgg cgagatgcag aggctgcaaa agggacagac
     1801 accaatcacc aacccaacca ttgcggttgt gcgagttcgc tacgagcagt tcctagaggc
     1861 ttacaaccac cataaaatat tgcagcagaa cacgcgcagg ctgcttaacg atgttaacaa
     1921 ccttagggtt actgccgata gcatcatcct aagtatttgg aacgaggtgg aagactactt
     1981 taaggattta cccgacgaag aacgccggtt gaaagcttcg gagtatgggg tagtttacgt
     2041 ttaccgcaag aacgagataa aggatttccg gttagaagac aggacgctgc taaagctatt
     2101 caactaacgc gcattcacct tcacactgat ccacatggac gctaccggga agctgcatgt
     2161 ttttagcagt tagcttttta ccatgttttg acttaccggc gactgccggg tgttggtttt
     2221 ccatataaat cgaagcagca acgctactgc caggaagcta acaattccaa tagcccaaat
     2281 gtcctgaagg ctaccaacct cttctatttc gtttacgctg atttccgaac ccataagcag
     2341 gaaggcaatt accgcaattg cattgttggt gaaatgggcc agcatgggga gccacaggct
     2401 gcgactccac tcatacaggt agccaaaaat gacacccagc gcaaaacggg gaatgaacc
//
LOCUS       DAOOQX010000277         2459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_52052_length_2459_cov_3.381032, whole genome shotgun sequence.
ACCESSION   DAOOQX010000277 DAOOQX010000000
VERSION     DAOOQX010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52052_length_2459_cov_3.381032"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PLX53_05320"
     CDS             complement(<1..768)
                     /locus_tag="PLX53_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY72536.1"
                     /translation="MNLLETHGVEKRFSNHLALDDISIAIPKGSIYGLLGPNGAGKTT
                     LIRIINQITGPDKGEVMFDGRKIEPNDIYRIGYLPEERGLYKKMKVGEQALFFSQLKG
                     LSRQDATKSLKYWFEKFEIQSWWDKKIEELSKGMAQKVQFIVTVIHKPELLIFDEPFS
                     GFDPINTQLITDEILELKKNGTTIIFSTHNMESVEQLCDHITLINKSKNILSGQIDQV
                     RANYGSNVFEVAFKGNLETLRQNLNGTFDIFNVNTEKA"
     gene            complement(929..1684)
                     /gene="rnc"
                     /locus_tag="PLX53_05325"
     CDS             complement(929..1684)
                     /gene="rnc"
                     /locus_tag="PLX53_05325"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOY72537.1"
                     /translation="MRKFLPKAFWRLRVPAADHEIYRSIEQVLGFSPQNLELYKLALV
                     HRSASLVIAHGKKVDNERLEYLGDAILDAIVADYLFTRFPSQNEGFLTQTRAKIVSRA
                     SLNQLAIGIGLPNIVVLGNGASFNQKNIFGNALEAIIGAMFLDMGFKETSRLVVSNIL
                     ERQVDLEAIQHVEIDFKSRLLELAQKQKNSAEFLTKIESLPESAIPHFTSVLRWDGKE
                     IAHGHGTSKKEAEQNAAMQAMETITEGADKPTV"
     gene            complement(1694..>2459)
                     /locus_tag="PLX53_05330"
     CDS             complement(1694..>2459)
                     /locus_tag="PLX53_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOY72538.1"
                     /translation="VSACASSTNGIISALDIIRTGKANVIIAGGSEASVNEVGVGGFN
                     SMQAISVNNDEYKTASRPFDATRDGFVIGEGGGCLILEEYEHAKARGAKIYCELKGGG
                     MSADAYHLTAPHPEGLGARKVMENALKDAGLTIKDIDYINVHGTATPLGDIAEAKAII
                     SVFGDYAFKLNISSTKSMTGHLLGAAGAIEAIAAIFAICTDTIPPTINFKSPDPEIDS
                     RLNITPNSAQKRSVKAALSNTFGFGGHNASIIFQKI"
BASE COUNT          629 a          554 c          575 g          701 t
ORIGIN      
        1 agccttctcg gtgttaacgt taaaaatgtc aaatgtaccg ttcaggtttt gcctcagggt
       61 ttcgaggttt cccttaaagg caacttcaaa tacgttgctg ccatagttag cccgcacctg
      121 gtcaatttgc ccgctaagga tattttttga tttattgatg agggtaatgt gatcgcaaag
      181 ctgctcaacc gattccatgt tatgggtgga aaagataatg gtggtgccat ttttcttcaa
      241 ctccagtatc tcatcggtaa tcagctgggt gtttataggg tcgaaaccgc tgaatggctc
      301 gtcgaatatg agcagctcgg gtttgtggat aacggttaca atgaactgca ccttctgggc
      361 catacccttt gagagctcct caattttttt atcccaccag gattgaattt caaatttttc
      421 aaaccagtac ttcaggcttt tagttgcatc ttgccggctt aaccccttga gctgggaaaa
      481 gaatagggct tgctctccaa ccttcatttt tttataaagc ccacgctctt cgggcaaata
      541 tcctatccgg tagatgtcgt tgggttcaat tttgcggcca tcgaacataa cttctccctt
      601 gtcggggccg gtaatctggt taattattct gataagtgtt gtttttccag cgccgtttgg
      661 gccaaggagt ccatagatag atccctttgg aatggcaatg ctgatatcgt ctagcgccag
      721 gtggtttgaa aatcgcttct ccaccccatg agtctctaaa agattcataa atctgatggg
      781 tttaagttca cctatgaata aaattatgca aatgtagcaa aagcaacgaa taatccttat
      841 aataaatgct tgatcagcac ctttgaagaa tgcgctcttg gatgcagcgc agagttatta
      901 gtagaaggaa gaggtaacga aaatgagcct aaactgtggg tttgtcagcc ccctctgtaa
      961 tagtctccat tgcctgcata gcggcattct gctcggcctc ttttttagaa gtaccatggc
     1021 catgggctat ttccttgcca tcccagcgaa gaacagaggt aaaatggggg attgccgact
     1081 cgggcagtga ctctatttta gttaaaaatt cggcagaatt tttttgcttc tgggccagct
     1141 ccagcagcct gcttttaaaa tcaatctcaa catgctgaat ggcttcaagg tcaacctgac
     1201 gttcgagaat attactaacc actaacctac tggtttcctt gaatcccatg tcgagaaaca
     1261 tggcgccaat aattgcctca agggcattac caaaaatatt tttttggttg aacgaggcac
     1321 cgtttcccag cactacgata tttggtaaac caattcctat ggctagctgg ttaaggctag
     1381 ctcggctaac aattttggcc cgtgtttgag taagaaaacc ctcattttgg ctggggaatc
     1441 gggtaaaaag gtaatcggct acgatagcat cgagaatggc gtcgccaaga tattcgagcc
     1501 gttcgttatc aaccttcttt ccatgagcaa taactaacga ggcagacctg tgaaccaagg
     1561 ccaacttata gagttctagg ttttgaggtg aaaatcccag tacctgctca atactacggt
     1621 agatttcatg gtctgcagca ggaactctca gacgccaaaa tgcttttgga aggaatttcc
     1681 gcaccggtag ctattaaatc ttctgaaaaa ttatagaagc attgtgccca ccgaatccga
     1741 atgtattgct aagcgctgcc ttaacgcttc tcttctgggc gctgttgggt gtaatgttta
     1801 ggcggctgtc aatttccgga tcgggtgatt taaagttaat agtgggtgga atggtatcgg
     1861 tgcagatagc gaaaatagca gcaatagcct caatggcacc ggcagcccca agtaggtgac
     1921 cggtcatgga ttttgtggag ctgatattga gcttgaaggc ataatcgccg aataccgaaa
     1981 ttatagcctt tgcctcggca atatcgccaa gaggtgtggc ggtgccgtgt acgttgatgt
     2041 agtcaatatc cttgatagta aggccagcat ctttcagggc attttccata acctttcggg
     2101 cgccaagtcc ttcggggtgc ggagctgtga gatggtaagc atcggccgac ataccaccgc
     2161 cctttaactc gcaataaatt tttgcacctc tggcctttgc gtgctcgtac tcctcaagaa
     2221 tgaggcaacc accaccctca ccaattacaa aaccatcgcg cgtggcgtcg aatgggcgag
     2281 atgctgtttt gtactcatcg ttgttgacag atatagcctg catggagtta aagccaccta
     2341 cgccaacttc gttgacggat gcttccgacc caccagcaat gataacattt gctttcccgg
     2401 tccgaattat gtcgagtgcc gaaatgattc cgttggttga ggatgcgcag gctgaaacg
//
LOCUS       DAOOQX010000278         2458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_52094_length_2458_cov_3.019559, whole genome shotgun sequence.
ACCESSION   DAOOQX010000278 DAOOQX010000000
VERSION     DAOOQX010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52094_length_2458_cov_3.019559"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLX53_05335"
     CDS             <1..609
                     /locus_tag="PLX53_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72539.1"
                     /translation="AEIPGGMYTNMLAQLKQLKLEKLLPRVLEIIPTVRLDAGCPPLV
                     TPTSQIVGAQAVNCIIDENKGLPFYTTKSTQFVNLVKGSYGKTPLPIDPQFRLKITGS
                     AEEVPFNTASYKKQPNPSFPEYGEGVKLAMNEKEELLLELFPNVTEKYLRDKRVAHFE
                     ELKRKKQEEERRRIEEEKQKYNSMTEQERWQRLQTGLISYYF"
     gene            complement(673..1626)
                     /locus_tag="PLX53_05340"
     CDS             complement(673..1626)
                     /locus_tag="PLX53_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOY72540.1"
                     /translation="MRKIKFLLLLIAPLVVLQCHSGRSKQKELIVFHAGSLSLPMKAA
                     ADSFMKLHPDVRILLEAAGSMECARKISELHKPCDLFVSSDYKVVEMLIDQGFATWCI
                     PFASNEMVIAYHDKSRLADCINGSSWPSVLLSKEVAFGRSDENSDPCGYRTIMTIRLA
                     EDFYHRPGLADSLLAKDVKHIRPKEVDLIALLEANVIDYLFIYKSVAQQHGLNYLELP
                     AAINLSSKEYESVYSRVSVEVVGSTPDARVALTGEPMVYGLTIPRNAPNFDIAVQFTA
                     FFLSENSGMRILSAMGQTPIIPTSTKTYNYIPQALRAFATP"
     gene            complement(1632..2012)
                     /locus_tag="PLX53_05345"
     CDS             complement(1632..2012)
                     /locus_tag="PLX53_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOY72541.1"
                     /translation="MAGSKGSKYYDVYLDYSIFLKRRGSNELVLSQEQIELLLLVDEL
                     QSITASARQMCISYRKAWELVRQSEQNLGFTIISKSRGGKNGGSSVLTPDGKLLANAY
                     RQLQADFNSSVKRVVKEFFQTINR"
     gene            complement(2091..2372)
                     /locus_tag="PLX53_05350"
     CDS             complement(2091..2372)
                     /locus_tag="PLX53_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HOY72542.1"
                     /translation="MFRTNEYFDGNVKSIAFETPDGRATLGVMAKGEYEFSTSTIEEM
                     TVVSGEMSVLLPGETSWKKYVEFETFIVPKDSRFRVRVDGDTAYRCYYR"
BASE COUNT          710 a          593 c          526 g          629 t
ORIGIN      
        1 gccgaaattc ctggaggaat gtacaccaac atgctggctc agcttaaaca gctaaagttg
       61 gaaaagcttc ttccacgcgt gctcgaaatt attccaactg tccgtttgga tgctggttgc
      121 cctccacttg ttacccctac gagtcaaata gttggagcgc aggccgttaa ctgcataatc
      181 gacgaaaaca agggacttcc tttctacacc acaaaatcaa cccaatttgt caacctggtc
      241 aaaggctcct acggcaaaac tccccttccc attgaccccc aatttcgtct gaaaattacc
      301 ggctctgccg aagaggtgcc ttttaacact gccagctaca agaagcaacc caacccttca
      361 tttcccgagt atggcgaagg ggttaagctg gccatgaacg aaaaggaaga actgttgctg
      421 gagcttttcc ccaacgttac cgaaaaatat cttagggata aacgtgtggc tcactttgag
      481 gagctaaaac gtaaaaagca ggaggaagaa cgtcggcgaa ttgaggagga aaagcagaaa
      541 tacaactcca tgacggagca ggagcggtgg cagcggctac agaccggatt aataagctac
      601 tacttctaaa attgcactgt tctagtgagg ccgaaggagc tatacaaaca gccttgatga
      661 gttgaatttg tactacgggg ttgcaaatgc ccgtaaagct tgtggtatgt agttataggt
      721 tttggttgat gtagggatta tcggggtttg ccccatcgca gaaagaatgc gcataccact
      781 attttcgctt agaaaaaacg ctgtaaactg cacagcaata tcaaaatttg gtgcattacg
      841 gggaattgtt aacccatata ccataggctc accggttaaa gctacccttg catcaggcgt
      901 gctaccaacc acctctaccg aaaccctgga gtaaaccgat tcgtactcct tactggaaag
      961 gttaattgca gcggggagtt caagatagtt tagtccatgt tgctgcgcca ccgacttata
     1021 aataaacaaa tagtcaatta cgttggcttc aagaagcgca atcaggtcaa cctccttagg
     1081 cctgatatgc ttcacgtcct tggcaagaag cgaatcggcc aggccaggcc gatggtaaaa
     1141 atcctcggca agcctaatag tcataattgt tcggtaaccg catggatcgg agttctcatc
     1201 gcttcttcca aaggctacct ctttgctcaa aagcacggat ggccagctgg aaccgttaat
     1261 acagtcggca agcctggact tatcatggta ggcaataacc atttcgttgc tggcgaaggg
     1321 gatgcaccat gtagcaaaac cctggtcaat aagcatttcg acgaccttat agtcggatga
     1381 tacaaacagg tcgcagggtt tatgaagctc cgaaatcttt ctggcacact ccatgctacc
     1441 ggctgcctca agcaggattc tgacatcggg atgtaacttc ataaagctgt cggcagccgc
     1501 cttcatggga agcgagaggc ttcccgcgtg aaaaacgatt agctcttttt gcttacttct
     1561 acctgaatgg cactgcaaca ccaccaacgg agcaattagc agtagtaaaa atttaatttt
     1621 tcgcatggtt gctaccggtt aattgtctga aagaattcct taaccacccg cttcaccgag
     1681 gagttgaaat cggcttgcag ctgcctgtat gcattggcca gaagcttgcc atcgggtgtt
     1741 agaacagagc tgccgccatt cttgccaccc cttgatttgc ttatgattgt aaaaccgagg
     1801 ttttgctccg actgcctcac aagctcccac gctttacggt agcttataca catctgccta
     1861 gccgaagcag taatggattg aagctcatca accaacaaca gcagctctat ttgctcctgc
     1921 gatagcacaa gctcattgct acccctacgc tttaaaaaaa tactgtagtc gaggtaaaca
     1981 tcatagtatt ttgagccctt tgaaccggcc ataatattcg ggtgagttta ctaaccaatt
     2041 tcaaaaacca gggtttaaga aactgactga aaaaggagta gccgattaaa ttacctgtaa
     2101 tagcaacggt aagcagtatc gccatccact ctaaccctaa accgggaatc tttaggaaca
     2161 ataaatgttt caaactctac atattttttc cagctggttt caccgggaag caaaaccgac
     2221 atttcacccg aaacaaccgt catttcctcg attgtagatg tgctgaattc atactcgcct
     2281 tttgccataa cacccagagt agccctacca tcgggagttt caaaggcaat tgactttaca
     2341 ttaccatcaa agtactcgtt tgtcctgaac atagccttat gttttcctca aatatatacc
     2401 aaaattgatt ttggtaatat accctaaact aaaagttttt taagttgtag agttctga
//
LOCUS       DAOOQX010000279         2455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_52177_length_2455_cov_3.007917, whole genome shotgun sequence.
ACCESSION   DAOOQX010000279 DAOOQX010000000
VERSION     DAOOQX010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52177_length_2455_cov_3.007917"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /gene="gldG"
                     /locus_tag="PLX53_05355"
     CDS             complement(<1..1521)
                     /gene="gldG"
                     /locus_tag="PLX53_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOY72543.1"
                     /translation="MELKKSYKVKSLLRLLVSVVAVAALYALSSVLHFRIDLTSDKLY
                     TLQPITREILKNLNGEVSMKIYLDGDLPTGFIRMKRMLTETLDEFNVASRGKVRYELV
                     NPYSAKNSREQDRIFAGLYDKGLAPTSVQQRNRQGGSSQTIVFPGLLMSYRGKEMAVN
                     LLKNNPMLSAEENLNLSIQNFEFALVDALVKLSTDSLPRIAFIKGHGELDEFETGDIE
                     KSLSEYFRVDRVILNGDMEALKPYSVAIVAGPTKPFPEEDKLVVDQFIMRGGKVLWFV
                     DPVAVSLDSLSTGASTLAFVAQTNLDDMLFRYGVRLNPFIVQDLQCAVIPVNIAPSGQ
                     ASKFAPAPWPYFPLLIPPSNHPVTRNLNPILTRFVSPVDTVGENPLVKKHYLLYTSAT
                     SRILQVPLLINLSEIEQTPNEQHFINPNVPVAAELEGYFTSVFTNRPLKNYNHGEPFE
                     FRAKSSFTRMVVVADADMVRNDVQHRPNGAYVLPLGYDRYTQQTYGNKELVMNMVKY"
     gene            complement(1546..2277)
                     /gene="gldF"
                     /locus_tag="PLX53_05360"
     CDS             complement(1546..2277)
                     /gene="gldF"
                     /locus_tag="PLX53_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOY72544.1"
                     /translation="MFAIFTRELQNFFSSLSGYIAAMVFLVVTGLILWVFPGNLNILD
                     GGYASLNGYFSIAPWVLLLLVPAATMRTFSDEKRFGTLELILTRPISDGRIVLGKFFA
                     TLVLVLIVLVPSLVYYLSVYLLSSPIGNIDAGGTWGSFIGLFFLASTYASVGIFTSSL
                     TDNAIVSFLLSVLFCLFLFSGLSALASLPLFSGVKPLLMFLSISEHYASISRGVLDSR
                     DLVYFISMIFLFIIFTRTVLESRKW"
BASE COUNT          726 a          570 c          566 g          593 t
ORIGIN      
        1 gtactttacc atgttcatca ccagctcctt gtttccgtat gtttgctggg tgtaacggtc
       61 gtatcccaac ggaagcacat aggctccatt agggcggtgc tgcacatcgt ttctaaccat
      121 gtcggcatcg gcaactacca ccattcgggt aaacgagctt ttagcacgaa actcaaaagg
      181 ttcaccatgg ttgtaattct tcaacggacg gttggtaaaa accgaggtaa agtatccttc
      241 aagctcggcg gctacgggaa cgttagggtt tataaaatgc tgctcgtttg gtgtctgctc
      301 aatctctgaa aggttgatta aaaggggaac ctggagaatt cggcttgtag cagaggtgta
      361 gagcaggtag tgttttttta ccaaaggatt ctctccaacc gtatctacgg gacttacaaa
      421 tcgggtgagt atggggttca gattccgcgt aaccgggtgg tttgacggtg gaattagtag
      481 cggaaagtag ggccatggcg caggggcaaa cttggaagcc tgaccggatg gagcaatgtt
      541 cacaggaatt acagcacact gcaaatcctg aacgatgaag gggtttaacc tcacgccata
      601 acgaaacagc atatcatcga ggttggtttg ggcaacaaag gccagggttg atgcacctgt
      661 tgaaaggctg tccaggctaa cagcaaccgg gtcaacaaac catagcactt ttccaccccg
      721 catgatgaac tggtcaacca caagcttatc ttcttcggga aaaggcttcg tgggtccggc
      781 aacaatagct acgctgtagg gcttaagcgc ttccatatcg ccatttagaa tcaccctatc
      841 aaccctaaag tactccgata gcgatttttc aatatcgcct gtttcaaact catctagctc
      901 tccgtgcccc ttaataaatg ctattcgcgg aaggctatca gttgaaagct tcacgagggc
      961 atcaaccaga gcaaattcaa aattctgaat agaaaggttt aaattctcct cagccgaaag
     1021 catagggttg ttcttcaaca ggttaacggc catctcctta cccctgtagc tcataagcaa
     1081 tccggggaat acaatcgttt ggctgcttcc cccctgcctg ttccgttgct gaacgctggt
     1141 tggtgccagc cctttatcgt acagaccggc gaaaattcta tcctgctccc tggagttttt
     1201 tgcactgtaa gggttaacca gctcgtagcg aaccttaccc ctggaggcaa cattgaactc
     1261 atccaacgtt tcggtcaaca tcctcttcat ccggataaag ccggttggta aatccccatc
     1321 gagataaatt ttcatggaaa cctcaccatt caaatttttg agaatttccc tggtgatagg
     1381 ctgaagggtg taaagcttgt cggaggttaa atcgatgcgg aaatgcaaaa cgcttgataa
     1441 agcataaaga gctgcaacag cgacaaccga taccagtaac cgcagcaaac tttttacctt
     1501 ataacttttc ttcaactcca ttttagcaat ccattttgca ttcccctacc attttctcga
     1561 ttccaaaacg gtacgggtaa aaatgataaa gagaaaaatc atcgagatga agtacaccaa
     1621 atccctacta tcgagtactc ctcggctaat agaagcataa tgctcgctga tgctaaggaa
     1681 cataagcaaa ggttttactc ctgaaaaaag aggcagcgaa gcaagagcgc ttaagcccga
     1741 aaataggaaa aggcaaaata gcacggacaa caggaatgaa acaatggcat tgtcggtgag
     1801 gcttgaggta aaaataccaa ccgaagcgta ggttgaggcc agaaaaaaga gtccgatgaa
     1861 tgatccccag gttccaccgg catcaatgtt tccaataggg cttgaaagca ggtaaaccga
     1921 aaggtagtaa accagcgatg gaaccagaac aatcaggacc agcaccagcg tggcaaagaa
     1981 tttacctaaa acaatccggc cgtcggaaat tggacgggtg agaattaatt ccaaagttcc
     2041 aaaacgcttc tcatcggaga aagttcgcat ggtagcagcc ggaacaagca gcagtagtac
     2101 ccagggagct atggaaaaat atccgtttag cgaggcataa ccaccatcaa gaatgtttag
     2161 gttgcccgga aacacccaaa ggattaagcc tgtaaccacc aaaaaaacca ttgcggcaat
     2221 gtagcccgac agcgaggaga aaaagttctg aagttccctg gtaaaaatag caaacatggt
     2281 agtttggttt ttttataaaa tttaatcaaa cactacctgg ttcccctgtc gggaggtcgt
     2341 tgtggaaatt ccattccagg gggtggtagg ttgggctcca ccatattgtt aagggttgtg
     2401 tcaaccctta tcctatccat atcaagctcc gtaagcttca caatgctaac accct
//
LOCUS       DAOOQX010000280         2439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_52696_length_2439_cov_3.303272, whole genome shotgun sequence.
ACCESSION   DAOOQX010000280 DAOOQX010000000
VERSION     DAOOQX010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52696_length_2439_cov_3.303272"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            321..2006
                     /locus_tag="PLX53_05365"
     CDS             321..2006
                     /locus_tag="PLX53_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOY72545.1"
                     /translation="MISFKKLTIRDILFRSRMAYAERPALAFVDSKPVTYANMAIIVR
                     NISGMLYTLGIRKGDRVALIGENSPNWGMAYLAATTIGAVVVPILPDFSDTELENIIR
                     HSEARVVFVSAKIYSHLKIDNLKGVEAFILLNSLVPIDEETFTADTTIPYSIPDPIVN
                     VDYCDCELPRPSEDDLAAIIYTSGTTGRPKGVMLTHRNLVSNVLSTLMVQEVTEHDRL
                     LSILPLSHTYECTIGFLIPMATGASVYYLDKPPVAQTLIPAMQKVRPTMVLTVPLIIE
                     KIFRGSIKPQLTSTALRQRLYQFSLSRRLLHRVAARKLHSTFGGELRFFGIGGAKLSR
                     EVELFLHEGRFPYSIGYGMTETSPLLTGSTPAQVRFRTAGYCLPGQELRLLNPNPETG
                     EGEILAKGSNVMVGYYKEPELTQAIFYEGWLRTGDLGVIDDKGYLTIKGRSKNLILSS
                     SGENIYPEEIEDIINNHEYVADSVVYESGGKLMARVHLNREAVAQYLAGIKSSAADLS
                     KNAEELTRRILDDIKVMVNSKVARFARLSVVHEQLEPFEKSATQKIKRFMYQD"
BASE COUNT          648 a          559 c          518 g          714 t
ORIGIN      
        1 cgttgctaag tcacactacc attactatcg tgatatcagc aggttgaata gcttccgcag
       61 ctcattccgg catcctaaaa ccaacagtgc aaaagttatc taccatagaa gcgttctttt
      121 tgattttttc atactccgca ggcggaaatt ttccgagctg aagttttagt cttttgtcag
      181 cctttttacc ttttttagga taggggcgta tgttaaaaat atgaaaaaaa tcgccttttt
      241 tgataatcta atcctttaaa agataccttt gcagctcaac tttacacatt agccatttag
      301 ctaaactgca gcagtaccaa atgatctcat ttaaaaaact taccattcgc gatattttat
      361 tccgttcacg aatggcctac gccgaaagac cagctcttgc ttttgttgac tcaaagcctg
      421 ttacctacgc caacatggcg attattgtca ggaacatttc cgggatgctc tacacccttg
      481 ggattaggaa gggcgatagg gtagccctca taggcgagaa tagcccaaac tggggtatgg
      541 cttatttggc ggcaaccaca ataggggcgg ttgtagttcc cattcttccc gacttttcgg
      601 atacagaatt ggaaaacatc atacgccata gcgaggcgag ggtagttttt gtttcagcca
      661 aaatttattc tcaccttaaa atcgacaatc ttaaaggcgt tgaggccttc atcctgctca
      721 acagtttagt tcctattgac gaggaaacat ttacagccga tactactatt ccctactcca
      781 tacccgaccc tattgtaaac gttgattact gcgattgcga gttaccccgc cctagcgagg
      841 acgacctggc agcaattatc tatacctcag gcaccactgg taggcccaag ggggtaatgc
      901 ttacccaccg gaaccttgtt tctaacgttc tatccacctt gatggttcag gaggtaacgg
      961 agcatgacag gctactctcc attctccctt tatcgcacac ctacgagtgt accattggtt
     1021 tcctaattcc aatggcaacg ggtgcatcgg tttactacct cgataaacca cctgtagccc
     1081 aaacacttat tccggccatg caaaaagtta ggcctacaat ggtgcttacg gttccgttaa
     1141 ttattgaaaa aatatttcga ggtagcatca agccccagct aacctcaact gccctaaggc
     1201 aacgtctata ccagttttcg ctaagtcgca ggctccttca tcgggttgct gcgcgaaaac
     1261 tccatagtac tttcgggggt gagcttagat tctttggaat tggcggagca aaactttcgc
     1321 gtgaggttga gctattcctg cacgaaggcc gatttcccta ctccataggc tatggaatga
     1381 cggaaacatc gccgttgctt accggtagca ctcccgcaca agtaaggttt agaacggcag
     1441 gctactgcct acccgggcag gagctgaggt tgcttaatcc caaccccgaa accggtgagg
     1501 gcgaaattct tgctaagggt tccaacgtta tggttggcta ctataaagag cccgagctaa
     1561 cccaagcaat tttttacgag ggttggttaa gaaccggaga ccttggagtt atagatgaca
     1621 agggatattt aaccattaag ggacggtcta agaatttgat actgagttct tcgggcgaaa
     1681 atatataccc cgaggaaatt gaggatatta tcaacaacca tgaatacgtt gccgattccg
     1741 tggtttacga gtcgggaggc aagctgatgg caagggttca cctcaaccgg gaagcagtgg
     1801 cgcaatacct cgccggcatt aaatcctcag ctgcggacct atccaaaaat gccgaagagc
     1861 ttaccagaag aattcttgac gacatcaagg ttatggtgaa ctctaaagtg gccaggtttg
     1921 cccggctaag cgttgttcac gagcagcttg aacctttcga aaaatcggcc acccagaaaa
     1981 ttaagagatt catgtatcag gactagtcat tgtagaactc gtcggatgcc gtttccgttt
     2041 ccaggttgag gtgtgttcta gaattgacaa tgatggagtt gtaattgcag attcccatgc
     2101 tttttcctgc aatttttagt ttgcctactt tcagataaat attttaggtc gcggttttgg
     2161 caagttcgac cgtttttgag cattcattcc ctgaaacata tttctgactt ttttttatta
     2221 taccgattaa gtcggtggct tttgtcattt tttgtttcat tcgtcaaatt ggccataacg
     2281 cgtgttatag ggcttttttt attatcattt tgttattgtt tccatagttt ttgcaacaaa
     2341 ttctttataa tcataatatt catttttgtc aacagaccaa gcaactctcg aatcactacc
     2401 tggatgcgaa acaggattat tattcctcct attgaataa
//
LOCUS       DAOOQX010000281         2429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_53010_length_2429_cov_3.374895, whole genome shotgun sequence.
ACCESSION   DAOOQX010000281 DAOOQX010000000
VERSION     DAOOQX010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53010_length_2429_cov_3.374895"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            623..>2429
                     /locus_tag="PLX53_05370"
     CDS             623..>2429
                     /locus_tag="PLX53_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72546.1"
                     /translation="MSAQNLDLKINAARLLLGNFEINSCKAENGRLNLKTNLLKKNDI
                     NPANGQAPLLQNEFFINRFTLSSFKALIEDTLSRQKSLIGIDRLTLNIRAKKILTLNL
                     DCQLKEINLGSKFMLNEQTQVNAIASYDGKSVFLRNVKISLSGSRLELQGLYHLENST
                     LGLGFKLNRTNINRLIQVANIKGLPADITGKVAMSGKLSYNGGKKNLTGLWIDYDAQN
                     VSIIAKGKIIEIKEINGLSYFTNNLQTHSGQVSNAVVRYENIEAAFSGRTYGTNRIII
                     LAEGLLKLGKNEPSFTNKLTGLEGNGTFKALLELRKGNGLQSKHFGFLLHDVASEISF
                     SLDSISAFKNLSNISGKATIRDKAIISATCYLDSSKIDFTLCQNNAIYYLNNPLNFSP
                     SIYLNSSYLNLDSLIKSVSTVNKGGKKRVYTTVETSINTKKLIYTNYAFENVTGVMNI
                     VGDSIYVKGLKATGFEGKIDGDLMVVGDKYSINASMEGIDITELFRHYNSWNQKYITH
                     NNIEGHLSGHCNFDFELDSANNIRWDRAKMNSEITINDGKLRGMNNIKKLSKWLKLDQ
                     VKSIDFTTIHNNILISNGCIYIPSMDLNSNVMGLNL"
BASE COUNT          861 a          397 c          462 g          709 t
ORIGIN      
        1 ccaacatcta tttaacattt agggattttg ccaaaatgta taggattagg aagtatttat
       61 aatatgccgg ttgctattag cgtggtatgt caaaatatta gattataaaa catctcgcta
      121 gagtcaccgt tcaattataa acaaatacat attttctata ctaaagccat accgctatca
      181 atattgggta gcaggcttaa caatggtcaa actacctggc gttacctgct ggctgggtga
      241 aaccaaaata tggatgttca ttccgctcaa cgccttttaa aataaggtta agagcggaag
      301 tgtttgtaac cggaaaactg aaagcatagc aagaatgaaa ccgctgttta attttttagg
      361 ccgatttgct cgcaaaggat tactttttac cctttacttt ttaagtggta taattttagc
      421 actaatattg cttacaactt atgtatttat aaatcagagc aaattgattg atttgttatt
      481 aaaacagttt aatgaatatg agttagtaag catatactac agtagtatta agtttgatac
      541 gtttaaaaat tttccatttg caaccttttc attttcagac ttcatactta cactaccaga
      601 aaatgggaat aatgatacgc tcatgtcggc gcaaaacttg gatttaaaaa ttaatgcagc
      661 aaggctactg ctggggaatt tcgaaattaa ttcatgtaaa gcagaaaatg gaaggctaaa
      721 tctaaaaaca aacctactaa aaaagaacga tataaatcct gctaacggac aagcacccct
      781 tctccaaaac gagttcttca tcaataggtt tactctttcg agctttaaag cattaataga
      841 ggatacactt agcaggcaaa aatcattaat cggaatagat aggcttactc ttaacataag
      901 agcaaaaaaa attttaacgt taaaccttga ttgccagctt aaggaaataa atttaggaag
      961 taagtttatg ctaaatgaac aaacacaggt aaatgcaata gctagctatg atgggaaaag
     1021 cgtcttcctg cgaaatgtaa aaattagcct aagtggtagc agattagaac ttcagggatt
     1081 gtatcacctg gaaaacagca cccttggcct gggttttaaa ctgaacagga caaacattaa
     1141 ccggttaatc caagttgcca atataaaggg attgcctgcc gacataacgg gtaaggtcgc
     1201 aatgtcgggc aagctgtcgt ataatggagg taaaaaaaat ttaaccggcc tatggataga
     1261 ctacgatgcc cagaatgtaa gtattattgc taaaggtaaa attattgaga ttaaagaaat
     1321 taacggctta agttacttta ccaacaacct gcaaacacat tccgggcaag tatcaaatgc
     1381 agtggttcgt tacgagaata tcgaagctgc tttttcgggt agaacctatg gaacaaatcg
     1441 aataataata ctcgccgagg ggttattaaa gctgggaaaa aatgaacctt catttactaa
     1501 taaactaact gggcttgaag gcaacggtac ctttaaggct ttactagagc taagaaaggg
     1561 taacgggcta caaagtaaac attttggatt tttgttgcat gatgtagcca gcgagattag
     1621 cttcagcctt gattcgattt ctgcattcaa aaatttgtct aacatttcgg gaaaagcgac
     1681 aattcgcgat aaggcaatta ttagcgctac gtgctatttg gatagctcaa aaattgattt
     1741 tacattgtgt cagaataatg ccatttacta tttaaataat ccgttaaatt tttctccatc
     1801 aatatattta aattctagct atttaaactt agatagctta ataaaatcag tttctaccgt
     1861 aaataaaggt ggtaaaaaaa gggtttatac gacagttgaa acaagcatca acactaaaaa
     1921 acttatatat accaactatg cctttgaaaa tgtaacggga gtaatgaata tagtcgggga
     1981 tagtatatat gttaaggggc taaaagctac ggggtttgaa gggaaaattg atggtgattt
     2041 aatggtcgtt ggtgataagt actctataaa cgcatcgatg gagggtatag acattaccga
     2101 acttttcaga cattataaca gctggaacca aaagtacatt acccataaca acatagaagg
     2161 gcatctctcg gggcattgca actttgattt tgagctggat agtgctaaca acattaggtg
     2221 ggacagggca aaaatgaatt cggagatcac tataaacgat ggcaaattga gaggcatgaa
     2281 taacataaaa aagctttcaa aatggcttaa actcgaccag gtaaaatcta tagattttac
     2341 cactattcac aataacattt tgatttcgaa tggctgtatt tacatcccct caatggattt
     2401 aaattcaaac gttatggggt taaaccttt
//
LOCUS       DAOOQX010000282         2429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_53014_length_2429_cov_2.919966, whole genome shotgun sequence.
ACCESSION   DAOOQX010000282 DAOOQX010000000
VERSION     DAOOQX010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53014_length_2429_cov_2.919966"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLX53_05375"
     CDS             complement(<1..471)
                     /locus_tag="PLX53_05375"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HOY72547.1"
                     /translation="MEKKKIVDVHEVKKVVNLGPIVGDGVAHLIMSALRFNKLNEKYS
                     NISHLKGLEFLSAAMDTFEITYEVNPEELKRIPRTGAFISVSNHPFGGVDGMILIKMM
                     LEQQRTDIKILSNFIIQRIPQIGEFVFPVDPFDNGVKRGSNLIGLKKALTHVSNG"
     gene            888..1733
                     /locus_tag="PLX53_05380"
     CDS             888..1733
                     /locus_tag="PLX53_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72548.1"
                     /translation="MKKFAVTLAILFGVANLYAQRNLVRPEDVKTFMASKTYVVLEEN
                     PTSEYNIEIREAVERSWKITPYEFITSKEFEKVRQDIDKSFLVLLQVRFDNDKVAPVY
                     NFLSVSLGAAVKKLTDMPDICSVPLSYKEVSEDTYTYKLEGLINFMQNYIRDINTNPK
                     LIKKDAFKLYNKNMKQIKNKELWVTESSLDKTTRALTDIRNNYKYSIKVVKPEDIQKA
                     ILERNPNVVFLHKVGPEGTRLQWRVWKMIIGAGDGMMYYYDMHTISKSAGDGFLVKDF
                     KKIGS"
     gene            1810..>2429
                     /locus_tag="PLX53_05385"
     CDS             1810..>2429
                     /locus_tag="PLX53_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY72549.1"
                     /translation="MADEKIIFSMVGVCKTFPPQKKVLNNIYLSFFYGAKIGIIGLNG
                     SGKSTLLRIIAGVEKSFDGEVVFSPGYSIGYLEQEPHLDESKTVKEVVQEGVAGTVKL
                     LEEYEELNARFAEPMDDEQMNRLIQRQGELTELIGHADAWNLDSKLERAMDALRCPEG
                     STPVKILSGGERRRVALCRLLLQEPDILLLDEPTNHLDAESVQWLET"
BASE COUNT          721 a          536 c          529 g          643 t
ORIGIN      
        1 cccattgcta acatgagtta aagccttttt gagccctatc agattcgatc cacgcttaac
       61 tccgttgtcg aatgggtcaa cagggaacac aaactctcct atctggggaa tacgctggat
      121 gataaaattt gacagaattt ttatatccgt gcgctgctgc tccagcatca tcttaataag
      181 aatcataccg tcaaccccac caaacggatg gttcgacacc gaaatgaacg caccggttcg
      241 gggtattcgt ttgagctcct cggggttaac ctcgtaggtt atctcaaagg tatccattgc
      301 tgccgaaagg aactccaatc ctttcaggtg ggatatattt gagtactttt cgttcagctt
      361 gttgaaccgc agggccgaca ttatcaaatg ggctacccca tcaccaacaa taggccctag
      421 gttgaccacc ttttttacct cgtgaacatc cacaattttc ttcttctcca ttgctcccag
      481 gttttctgaa tttgaatgag aatgtatgca gataagttga tatatagcaa tttatactgg
      541 taactgccac catttccacc gttcaaatct acataaaatt taccgattta gcttttttga
      601 acttagctcc gttcaagctt accctggagg gaaacatttt ttgttttcag gttagcccta
      661 attttgttaa ggaacctttg attaaatcat gaatttttaa aaaaagaagt ataaatttgc
      721 aatctataaa tacagaggta aagaacctgg aatcatgcat aatctcattt tgccgggcca
      781 aatggtagag attaaccagc ttcagaaaat ttagttcagg ttgacttccg gcgaattgct
      841 ctttaaggaa ttatttgtaa attacgtggt taaaaatgtc taagctcatg aaaaaatttg
      901 cagtaacatt agcgattctt tttggagtgg caaacctcta tgcccaaaga aatctggtaa
      961 gacctgagga tgttaaaaca ttcatggcgt cgaaaactta tgttgtgctt gaggaaaacc
     1021 ctacatcgga gtataacatc gaaattcgtg aagctgttga gcgctcatgg aaaattacac
     1081 cctacgagtt catcacatcc aaggagtttg aaaaggtgcg tcaggatata gacaagtctt
     1141 ttctggtgct gttacaggtt aggttcgaca acgataaggt ggcacccgtt tacaacttct
     1201 taagcgtttc gctaggggct gccgtcaaaa agttaaccga tatgcccgat atatgctcag
     1261 ttcctttaag ctacaaggag gtttcagaag atacctacac ctacaaactc gaagggttga
     1321 ttaacttcat gcagaactac atcagggata tcaacaccaa cccaaaactc attaaaaagg
     1381 acgcctttaa gctctacaac aagaacatga agcaaattaa aaacaaggag ttatgggtaa
     1441 ccgaaagcag tcttgataaa accacccgtg ccctaactga catcaggaac aactataagt
     1501 actccatcaa ggttgttaaa cccgaggata ttcagaaagc cattttggaa cgcaacccga
     1561 atgtagtatt cctacacaag gttggtcccg agggaacaag gctgcaatgg cgcgtctgga
     1621 aaatgattat tggcgctggc gatgggatga tgtactacta cgacatgcat accatctcga
     1681 aatcagccgg tgatggattc ctggttaagg acttcaaaaa gattggcagc taaatagcat
     1741 tacactaacc aaccgctggt taacatgctt cgccagcggt attttttata ccctgtaaca
     1801 gcgaagtaaa tggccgacga aaaaattatt ttctccatgg ttggggtgtg taaaacattt
     1861 ccacctcaaa aaaaggtgct gaacaacatc tacctgtcgt tcttctacgg cgccaaaatt
     1921 ggaataattg ggcttaacgg ctccggaaag tcaaccctgc tacgaataat tgccggggtt
     1981 gaaaaatcct ttgatggcga ggtggtattc tcgccgggct attccattgg ctaccttgaa
     2041 caggaaccac atctcgacga aagcaagaca gtaaaggagg ttgttcagga aggtgttgcc
     2101 ggaacagtta aacttcttga agaatatgag gagttgaatg cccgatttgc cgagcccatg
     2161 gacgacgagc aaatgaatag gctcatccaa aggcagggtg aactcaccga gttgattggt
     2221 catgccgacg catggaatct cgattccaag ctggagcgag ccatggatgc acttaggtgc
     2281 cccgagggca gcactccggt taaaatcctc tcaggcggtg aacgtcgtag ggtagcccta
     2341 tgcaggctgc tgctccagga acccgatatt ctcctgctcg atgagcctac caaccatctc
     2401 gatgccgaat cggtgcagtg gctcgaaac
//
LOCUS       DAOOQX010000283         2424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_53158_length_2424_cov_2.572816, whole genome shotgun sequence.
ACCESSION   DAOOQX010000283 DAOOQX010000000
VERSION     DAOOQX010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53158_length_2424_cov_2.572816"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            447..1124
                     /locus_tag="PLX53_05390"
     CDS             447..1124
                     /locus_tag="PLX53_05390"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOY72550.1"
                     /translation="MKKYLFILVGVAISVFASAQNTDSRDITPFDKLKVIGEVKVYLQ
                     RDSVEHISVKTYDVPTSDIHTEVKGKTLEVALKANIYKSVKADIYVYYRELRDISLSA
                     AASISLNDTLTGDKVVINANTSSEFDGGLNVETADIVVGQGSTVRVRGAVKNYEAKVN
                     TGGVLSALDLVSDSTFVTVSTGAIAKVNASRLIDAKVRTGGSLTYTGNPKQKSINTLM
                     GATIVEQ"
     gene            complement(1143..2096)
                     /locus_tag="PLX53_05395"
     CDS             complement(1143..2096)
                     /locus_tag="PLX53_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOY72551.1"
                     /translation="MIWQFVLDKLKSGQSMYLIVVSETLGSSPGKVGFKMAVSSDGEI
                     SGSIGGGVMEFRMVELARKELAVGGKNTFLKRQVHSSEAEEDKSGMICSGEQTHIFIY
                     LSTLNLKICSDIVAAISSGKTGLLHIEPKRLEFKSAGVAELPAKHEFNHNGNSWYYSE
                     VIGIKDRAYIFGAGHISLPLTHILSLLDFWVEVYDNRKELVTFTSNNLANKKEVIDFD
                     NASKYVMEGNSTYCLIMTFGHKFDEMILRQLLPLKVGYIGMIGSKSKVKAIFDNLLEE
                     GYSPDQLAKVCSPVGLPIGSQTPAEIAVSIAAQIVQWRNGL"
BASE COUNT          670 a          501 c          545 g          708 t
ORIGIN      
        1 tttctgttgg gtaacgtaac gctgcaaccc ggttaggttt tgttttactt cattcatctg
       61 gtcaccctta ttgtctgctt caacctgttt cgactgtcct aaacaggttg aaaaaatggt
      121 gaaaaaaaaa gctatggtaa ttataatccg ttgaaccatg atgttgtggc tactacttat
      181 tacgaaaacg aatatttttc tgttttgttc acgctagtga tttactttaa aacggcaagg
      241 cgaaaatgta atggtgaaaa tagctgcatg ttggttattt tatgttaaag tttgcacttt
      301 tacaacttag catgggtaca tttgcataga agttgtgaaa ggttataacg gcaaacttct
      361 gtatcttaag taggtttcaa ttgaaaaata ttggcaattt taccatgtat tatttcttgg
      421 actaaattca taaaagctta atacctatga agaagtatct ttttattcta gttggcgtgg
      481 caatttcggt atttgccagt gcgcagaata ccgacagcag ggacataacc cctttcgata
      541 agcttaaggt gataggagag gtaaaggttt acctgcaacg agatagcgta gagcacatca
      601 gcgttaaaac atacgacgtg cctacctcgg atatacatac agaggttaag ggaaaaactt
      661 tggaggtggc tctcaaggcc aacatttaca agagtgttaa ggctgatatt tacgtgtact
      721 acagggaatt aagggatatt tcactttcag ctgctgccag cataagcctg aatgatactc
      781 ttacaggcga taaggttgtc ataaatgcca acaccagctc tgagtttgac ggtggtttga
      841 atgttgagac agccgatatt gttgtggggc aagggtcaac agttcgtgta aggggtgctg
      901 taaaaaacta tgaggctaaa gttaacaccg gcggagtttt gtcggctctc gacctggttt
      961 ccgatagcac gtttgttacg gtgagcactg gcgctattgc caaggtaaac gcctcccggc
     1021 ttattgatgc caaggtacga acaggaggca gcctgaccta tacaggtaac cctaaacaaa
     1081 agagtattaa tacattaatg ggggcaacca tagtggagca gtaggcctct tccagaaaaa
     1141 ttttacagtc cgttccgcca ttgaacaatc tgggcggcaa tgctgacagc tatctcagcg
     1201 ggtgtttgac tacctattgg tagcccaact ggcgagcaaa ctttggccag ctggtcaggc
     1261 gaatagcctt cttctagcag gttgtcaaat atggctttaa ccttgctttt actgccaatc
     1321 atgccgatgt atccaacctt aagcggaagt agctgcctga gaatcatctc gtcgaactta
     1381 tgtccaaaag tcatgatgag gcagtaggtg ctattgccct ccattacata ttttgaggca
     1441 ttgtcgaaat caattacctc ctttttattt gcaaggttat tggaagtaaa ggttaccagc
     1501 tccttacggt tatcgtaaac ttcaacccaa aaatcgagga gcgaaagaat atgggttagg
     1561 ggaaggctta tgtgtccggc tccaaaaatg taagccctgt ctttgatacc aataacttcg
     1621 gagtagtacc agctgttacc gttatggttg aattcatgtt tggcgggcaa ttctgctacg
     1681 ccagcactct taaattctaa cctcttaggc tctatgtgga gtaagccggt tttgccggag
     1741 gatatggctg ccactatatc gctgcagatt ttcaaattca acgtggacag gtaaatgaaa
     1801 atatgggttt gctcacccga gcaaatcatc cccgatttat cctcctcagc ctcactgcta
     1861 tgcacctgcc gttttagaaa agtattttta cctcctacag ccagctcctt acgggccagc
     1921 tctaccatgc gaaactccat aacaccgcct cctattgagc ctgatatttc gccatcggac
     1981 gaaacggcca ttttaaaccc cacctttcca gggctactac ccagcgtttc ggataccaca
     2041 ataaggtaca tgctttgacc cgactttagc ttatcgagca cgaattgcca tatcatcttt
     2101 tccgtttttt aaagacctgc taagtaggtg aaatttgttg aaatttcata gccttcaagt
     2161 tcatttctac tcgaagcatt attttttatc attgccctaa atgctgaaga gttggattga
     2221 ttggctttaa agttaaaagc ccattccctg ctaccgcccg agcctaccca ccagccggcg
     2281 gagagctcaa cgaagttagt cctctcgtgt ggtagtatag aaaaggaaag tagtgggtag
     2341 gaattagtaa atggataatc cggggggatt atgctttatg aatttaagga tgggagtact
     2401 aggtgctctg gttgagaggc agaa
//
LOCUS       DAOOQX010000284         2420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_53285_length_2420_cov_3.058351, whole genome shotgun sequence.
ACCESSION   DAOOQX010000284 DAOOQX010000000
VERSION     DAOOQX010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53285_length_2420_cov_3.058351"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            805..1362
                     /locus_tag="PLX53_05400"
     CDS             805..1362
                     /locus_tag="PLX53_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOY72552.1"
                     /translation="MSVFTLILLALGLSFDTLAVSISSGIARKQIVFFEAFRLAAVLG
                     FFQAAMPILGWLGGISIKKYIEPVDHWVALALLVIIGVKMIIESFKDDGVKTFNPLEP
                     RVMVGMALATSIDALAVGVSFAITQVNLILAFFVFGSITFLVAMLGMLFGKKIGNHIG
                     HRMEIIGGLILIGIGVKIVLEHLNG"
     gene            complement(1365..2060)
                     /locus_tag="PLX53_05405"
     CDS             complement(1365..2060)
                     /locus_tag="PLX53_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOY72553.1"
                     /translation="MSYTVLKYLVEKNRQPNNTQKEPGPVVTISRQMGCPGNRVTNLL
                     VQELNQRYCLKGESQWNGLAKEKIEQIAAERLGLPKEEIDYVFEAKRKGAMEEILHSM
                     STKYYKSDQRIQNTVKGVIRMEAEKGRVVILGRGGVAITRDIPRSLHIQVEAPFEWRV
                     LRVEEMYHFDNKSAQLYVNEIDKKREAMRVYFGGKGTDYTRFDLTFNAMTLSVKEIVD
                     IIIYTMEVRKLID"
BASE COUNT          612 a          550 c          509 g          749 t
ORIGIN      
        1 gaatatcggg gttgtgctcc ctgactgcct gcatggcctg gtcaatattc tcggatgctc
       61 ctaacacttg aatatccttg tcgaacgaaa gcatctgaac caggatgtcc ctggagttgg
      121 cctcgtcatc aactacgaca gcagttatag ttttgcccat tatacttagg tatattccaa
      181 acttcaaagc cgagtgatcc agctcatttt ttacccgtgc aaaattttat tgctttaacc
      241 ccgtaagcta ctattttgtt atatgcactc gtttatgttc cgatgaaccc caaagggttt
      301 gccgtaagca aacctccatt aaccggcttc gctgaactgg cataaaacgc ttggtagttt
      361 attcgctctt gtacaagcca tctacatggg cgtttcataa ttatgcaatc ctgctgccac
      421 aaaatttttg aggtaagcta ccatcaactt caaaagtagc tattatttga taattttctc
      481 caacaagcca tcttaaattc atcctggcaa ccatgttgct ggcgtaaggg gttgacagcc
      541 aagcatgttc accgctgccg ggccatacaa accctccaaa tatttttggc tgagcaaccc
      601 gagttacagg ggtcttcaat tttttagttg cacagcttga cgttaatggc aatatgacta
      661 gttcaattta aaaaaatgcg aaaagtaagg agttgaataa gaagtatata atgttgatat
      721 aaaagcaaaa gcgctcaaat caaaataatg ggtttgctgt acggtattgg ttgttatgtt
      781 taactttgcc gccaaaagca attcatgtca gtattcaccc ttattctcct ggctttgggc
      841 ctttcgttcg ataccctggc tgtttccatt tccagtggaa ttgcacgtaa gcagatagta
      901 ttttttgaag ctttccggtt ggctgctgtg ctgggattct tccaggcagc catgccgatt
      961 ttgggttggc ttggcggaat atccataaag aagtacattg aaccggttga ccactgggtg
     1021 gcgctcgccc ttttggtcat tataggcgtt aaaatgatca ttgagagctt taaggatgat
     1081 ggggtaaaga catttaaccc gctagaacca agggtgatgg taggaatggc gttagccacc
     1141 agcatagatg cgctagccgt tggagtaagc tttgcaataa cgcaggtgaa ccttattctg
     1201 gcatttttcg tctttggctc cattaccttc ctggtagcca tgctgggaat gctgtttggg
     1261 aagaaaattg gcaaccacat tgggcaccgt atggagatca taggagggct aatacttata
     1321 gggatagggg taaagattgt gctcgagcac ctgaatggct agcgctaatc aatcagctta
     1381 cgcacctcca tggtgtagat gatgatatca acaatttcct taacggagag ggtcatagcg
     1441 ttaaacgtta ggtcgaaccg ggtgtagtcg gttcctttcc cgccaaagta aacccgcatg
     1501 gcctcgcgct ttttgtctat ttcgttcacg tagagctggg ctgacttatt gtcaaaatgg
     1561 tacatttcct caactctaag cactctccat tcaaagggtg cctcaacctg gatatgtaac
     1621 gagcggggaa tatcgcgggt aatggccact ccacctcttc ccagtatcac cactcgtcct
     1681 ttctccgcct ccatccggat tacacctttt accgtgtttt gaatccgctg gtcgctcttg
     1741 tagtatttag ttgacatgga gtggagtatt tcctccatag ctccttttcg tttggcctca
     1801 aacacgtagt cgatttcctc ctttggcaaa cctaatcttt cagcagcaat ttgttcaatt
     1861 ttctctttcg ccagcccgtt ccactggctt tctcctttaa ggcagtaacg ctggttaagc
     1921 tcctgaacca gcaggttggt aaccctattg cccggacacc ccatctggcg cgaaatggta
     1981 accaccggcc ctggttcttt ctgggtattg ttgggctgac gattcttttc aaccagatat
     2041 ttgagtacgg tatagctcat gggttaatgt ataaaatgat atttaacatt ggctaaatat
     2101 aacttttttt tcctattaag tcaataaatt ttctaaagaa actgtcatgt ttttgtaaac
     2161 ataaccaccc acaagcaacc gagcgttttt tgctgttccg gctaagccaa aacatgtgtg
     2221 ttccacttgc tcaacccttt tacctataag ttactggcta gggttcacac aaccagatat
     2281 ataaatattt tatgcggttg aattgctgtt taaagcctaa ttgatttcta atagcatttt
     2341 acccattttt tacttcccct gtaaacttca gctgatagtt acaccacaac cggaattttg
     2401 gtttcaggag ccgccatcat
//
LOCUS       DAOOQX010000285         2415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_53412_length_2415_cov_3.564831, whole genome shotgun sequence.
ACCESSION   DAOOQX010000285 DAOOQX010000000
VERSION     DAOOQX010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53412_length_2415_cov_3.564831"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLX53_05410"
     CDS             <1..436
                     /locus_tag="PLX53_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY72554.1"
                     /translation="NDQKASVLHNKGNALFKQNKLKESIEAYKSALKFNPNDKETKYN
                     LSEAIRLFQQQQQNQQDNNNKDKDKDKQNQNQNQPDNPKDEQNNNPDNRDSQGNNQQQ
                     PKLSKEDAERILEAIQNHEMQIQDRLKEEKAKQAKVRVGKNW"
     gene            454..2301
                     /locus_tag="PLX53_05415"
     CDS             454..2301
                     /locus_tag="PLX53_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOY72555.1"
                     /translation="MSKRFLLVAFIFVSSIIQAQNVQFEASAPNMVEVGEQFRLTYSL
                     NSKPSNFNPPTIAGFEVIAGPSTSSSTSIEIINGKVTQQQSVSYTYILEATAVGKYNI
                     PPAEAMVGKVTYQSNPVSIEVIKASSGSARQANQPQPTQPQQIAAGEENLPDDDLFVT
                     IELNRKTAYMGEPVEAAIKIYTRVNILGFEDAKFPTFDGFWSQELESSSNVQFERANV
                     NGKIYNVGIIRRYLLFPQKNEKLKIDPFELVVVYQGNAARPRSIFDEFFGGGFENYRK
                     RLVSKPVSVVVKSLPQGAPSSFYGGVGNFKINVGVDKNKLKTNEAVTLKLVVTGTGNL
                     KLMGTPKVEFPSTFEVFEPKVSDNINPSQQNASGSKTFEYVAIPRASGSYEIGPVDFT
                     YFDPSAEKYVSVQSKTVNLEVIPDSSATGAVVLTSGLSKEDVKFIGKDIRFIKTNPVR
                     LKPINSFVVGSLMYLFSYILVVILFAVFLFVFRKYRKQIQDTGFVRNKHAQKIAQKRL
                     QVAQEMLRLDEGEKFYEEIHKALWGYASDKLNLPVASLTTESVREALSGKGVSESAID
                     EFIRIIEACEYARFAPSSEHLQLETIYKDTYSLILQLEENLGKVKSRLW"
BASE COUNT          759 a          492 c          527 g          637 t
ORIGIN      
        1 aaacgaccag aaagcatcgg tacttcataa taagggaaac gcacttttca aacagaataa
       61 gctaaaggag agcattgagg cctataaaag tgccctgaaa tttaacccca acgacaagga
      121 aaccaagtat aacctttcgg aggcaataag gttgttccag cagcaacagc agaaccagca
      181 ggataataac aacaaggata aggataagga taaacaaaat caaaaccaga atcagccaga
      241 taacccgaag gatgaacaga ataacaaccc cgataacagg gattctcagg ggaataacca
      301 gcagcagccc aaactttcaa aagaggatgc cgaacgcatt ttggaggcca ttcaaaacca
      361 cgaaatgcaa atacaggatc gcttgaagga agaaaaagca aaacaagcaa aggttcgagt
      421 tggaaaaaat tggtaggcta accgaagttg aatatgagca aaagatttct actagttgca
      481 tttatttttg tatcaagcat catccaagcc cagaatgtcc agtttgaggc ttctgctccc
      541 aatatggttg aagttggtga gcaattccgc ttgacatact ctttaaattc aaaaccttcg
      601 aattttaatc ctcctactat tgctggcttt gaagttattg ctgggccaag cacctctagc
      661 agcacaagta ttgaaataat aaacggaaag gttacccagc agcaaagcgt ttcttacacc
      721 tatattctgg aagccaccgc tgtgggaaag tacaacatac cacccgctga ggcaatggtt
      781 ggaaaggtta cctaccaatc taaccctgta agcatagagg taattaaagc atcatccggc
      841 agtgcgcgac aagccaatca accacaacct actcaacccc aacaaattgc cgctggtgag
      901 gagaatttgc ccgatgatga cctgtttgtt actattgagc tgaatcgaaa gactgcctac
      961 atgggcgagc cagttgaagc cgccatcaaa atatataccc gcgttaacat tttgggtttt
     1021 gaggatgcta aatttcccac tttcgatggg ttctggagtc aggagttgga atcttcctcc
     1081 aacgtgcaat ttgagcgagc caatgtaaac gggaagattt ataacgttgg cattatacga
     1141 cgatacctgc ttttccccca gaaaaatgag aagttaaaaa tcgatccctt tgagctggtt
     1201 gtagtttatc agggaaacgc agcaagaccc cggtcaattt ttgacgagtt ttttggtggc
     1261 ggctttgaga attaccgcaa acggctggtt agcaaacctg tatcggttgt agtaaagagc
     1321 ttacctcaag gtgcaccctc ctcgttttat gggggtgtgg gcaatttcaa gattaatgtg
     1381 ggtgttgata aaaataaact taaaaccaac gaggctgtta ccctaaagct ggttgtaact
     1441 ggcaccggta acctcaagct aatgggaaca cctaaggttg agtttccttc tacctttgaa
     1501 gtttttgaac ccaaggtttc agacaacatc aatccatccc agcaaaacgc ttcaggcagt
     1561 aaaacttttg aatatgttgc catccctaga gcctcgggta gctatgagat agggccagtt
     1621 gattttacct attttgatcc ttcggccgaa aaatatgtat cagtacagtc aaaaactgta
     1681 aatcttgaag ttattcccga ctcctcggca acaggggcag tggtgcttac aagtggttta
     1741 agcaaggaag atgtgaagtt tataggcaag gatattaggt ttattaagac aaaccctgta
     1801 agattaaagc ctattaactc cttcgttgta ggttctctaa tgtacctgtt ctcctatatt
     1861 ttggttgtaa tcctctttgc tgtttttctg tttgtattca gaaaataccg caagcaaata
     1921 caggatactg gttttgttag aaacaagcat gctcagaaaa ttgctcagaa aaggttgcag
     1981 gtagctcaag aaatgctcag gttggatgaa ggagaaaaat tttacgagga aattcacaag
     2041 gccttatggg gttatgcatc cgataagctt aatttacccg tcgcctcgct tactaccgaa
     2101 agcgttaggg aagcactttc cggtaaaggt gtgagtgaat ctgcaattga tgagtttatt
     2161 aggattattg aagcttgtga gtatgctcgt tttgctccat catccgagca ccttcaattg
     2221 gaaaccattt ataaagatac ctactcgctt attttgcagc tggaggaaaa tctcggaaaa
     2281 gttaagtccc gactttggta aaatcttaat aacgtatgaa gcgattactt aatatagtgc
     2341 tgctggtttg tgctccatta tggagtttgc ttgcgcaaaa tcccagccag cttatgaagc
     2401 aggcaaacga gcttt
//
LOCUS       DAOOQX010000286         2396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_54031_length_2396_cov_3.781717, whole genome shotgun sequence.
ACCESSION   DAOOQX010000286 DAOOQX010000000
VERSION     DAOOQX010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54031_length_2396_cov_3.781717"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1643
                     /gene="speA"
                     /locus_tag="PLX53_05420"
     CDS             <1..1643
                     /gene="speA"
                     /locus_tag="PLX53_05420"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HOY72556.1"
                     /translation="EYGYKGQNFIIYPIKVNQMRPVVEEIINHGKKFNIGLEAGSKPE
                     LHAVIASNPDNESLIICNGYKDEDYIELALLAQKMGKKIFIVIEKQNELKLVARVSQR
                     LKIKPNLGIRIKLASVGSGKWEDSGGDVSKFGLTSSELIQALEFLEGNGMKDSLKLVH
                     FHIGSQVTKIRRIKLALREASQFYVQLYLQGFNIEFVDIGGGLGVDYDGTRSSNSESS
                     INYSLQEYVNDSVSTIVDVSDKNNIPHPNIITESGRSLTAHHSVLIFDVLETASLPSW
                     EENEDIGENPHELVSELYEIWDKLNQSKMLESWHDAQQIREEALDLFSLGLLDLKTRA
                     QVERLFWSIARDINQMTADIKHVPDEFRSLPKLLADKYFCNFSLFQSLPDTWAIDQIF
                     PIMPIQRLNEKPDRAATLQDVTCDSDGKIDNFISTRNISYYLPVHTVKPNESYYIGAF
                     LVGAYQEILGDLHNLFGDTNAVHVSVNDKGYEIDQIIDGETVAEVLEYVQYNPKKMVR
                     TLETWVTTSVKAGIISAKEGKEFLSNYRSGLYGYTYLE"
     gene            complement(1748..>2396)
                     /locus_tag="PLX53_05425"
     CDS             complement(1748..>2396)
                     /locus_tag="PLX53_05425"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOY72557.1"
                     /translation="GMEEQDSIFVHYIQKLFALPLSEEIRNKFAETLADGFAETDYFE
                     VIDYLAHAGGKAAATVFDNPDFKTRLELERPLMVGSKAHDFTAKLMEGEKFKLSRSVS
                     RYELIVFWSVWCPHCVETLPKIRSIYEEFRHKGFDVVAVNIDEEQEPWKEFTSNNSLQ
                     WHNTQVPFSSNSPIPEYYNVDGTPKMFLVDNSLRILSRPTSAAQLHATLRKLYGE"
BASE COUNT          617 a          562 c          596 g          621 t
ORIGIN      
        1 aggaatatgg ttacaaaggg caaaacttca tcatctaccc gatcaaggtg aaccagatgc
       61 ggccggtggt tgaggagata atcaaccatg gcaagaagtt taacattggg ctcgaggctg
      121 gttcaaaacc cgagcttcat gccgttattg ccagcaatcc cgacaatgag tcgcttataa
      181 tttgcaacgg ctataaggac gaggactaca ttgagctggc cctgctggcc cagaagatgg
      241 ggaaaaagat cttcatcgtg attgaaaagc aaaacgagct taagctggtg gctagggttt
      301 cgcaacgttt aaagattaag cccaacctgg gaattcggat taagctggca agcgtaggga
      361 gcggtaaatg ggaggactcc ggcggcgatg tgagcaagtt tgggcttacc tccagtgagc
      421 tgattcaggc gttggagttt ctggaaggta acggtatgaa agattccctc aagctagttc
      481 atttccacat tggtagccag gttaccaaga ttcgtcgcat taaacttgcc cttcgggaag
      541 cctcccagtt ttatgtgcag ctgtatcttc agggtttcaa catcgaattt gtggatattg
      601 gtgggggcct tggggttgac tacgatggaa cccgctcatc gaacagcgaa agcagcataa
      661 actacagcct tcaggagtac gtgaacgatt ccgtatccac catagttgat gtcagcgata
      721 agaataacat tccccatccc aacattataa ccgaatcggg acggtcgcta accgcccatc
      781 actcggttct catcttcgat gttcttgaaa ccgcctcgct accaagctgg gaggagaacg
      841 aggatattgg cgaaaacccg cacgagctgg ttagcgagct atacgaaatc tgggacaagc
      901 taaaccagtc taaaatgctg gagagctggc acgatgccca gcaaataagg gaagaggccc
      961 tcgacctttt tagcctgggg ctgctcgacc taaaaacccg ggcacaggtg gagaggctat
     1021 tctggtctat tgcccgggat atcaaccaga tgactgccga cattaagcac gttcccgacg
     1081 agttccggtc gcttcccaag ttgttggccg ataaatactt ttgcaacttt tcccttttcc
     1141 agtcccttcc cgacacctgg gctattgacc aaatattccc gattatgccc attcagcgac
     1201 ttaacgaaaa gcccgatcgg gctgctaccc ttcaggatgt tacatgcgat tccgacggta
     1261 agattgacaa ctttatttct acccgtaata tttcgtacta cctgccggtt cataccgtaa
     1321 aacctaatga gtcatactat ataggtgcat ttttggttgg cgcataccag gaaattctgg
     1381 gtgatctgca caaccttttt ggcgatacca atgctgtcca cgtatcggtt aacgataagg
     1441 gctacgagat tgatcaaatt attgatggcg agacggtggc cgaggtgctg gagtacgttc
     1501 aatataaccc caagaaaatg gtgcgaacgc tcgaaacctg ggttactacg tcggtgaaag
     1561 ccggtatcat ttctgccaag gagggtaagg agtttctatc gaactaccgc tcaggccttt
     1621 atggttacac ctacctggag tagccggttc gggttgacgc ccttcgtgga gatttagcca
     1681 aatatattgg ttttataaac agatagcagg gttgctactg ctgtttaccg gatattacct
     1741 gctattgtta ttcaccgtaa agttttcgta acgtggcatg tagctgggca gccgacgtgg
     1801 gtcgcgacag tatgcggagc gagttgtcaa ccagaaacat ttttggcgtt ccgtcaacgt
     1861 tgtagtactc cggtatgggg ctgttggagc taaaaggcac ctgggtgttg tgccattgca
     1921 aactgttgtt gcttgtaaac tccttccatg gctcctgttc ctcatcaatg ttaacagcca
     1981 ccacgtcaaa tcctttatgc ctgaactctt catagatact tctgattttg ggcagggttt
     2041 caacgcagtg gggacaccaa accgaccaga aaacaataag ttcataccgg gatacactcc
     2101 tagacagctt aaatttttca ccttccatga gcttagctgt aaaatcgtga gctttactcc
     2161 ctaccatcag gggacgctca agttccagcc ttgttttaaa atcgggatta tcaaaaaccg
     2221 ttgcggctgc tttaccgcca gcgtgcgcta ggtagtctat tacttcgaag tagtcggttt
     2281 cggcaaatcc gtcggctagt gtttcggcaa acttgttacg gatttcctct gaaaggggaa
     2341 gggcgaataa cttttggatg tagtgcacaa aaatggaatc ctgctcctcc ataccg
//
LOCUS       DAOOQX010000287         2388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_54319_length_2388_cov_2.477068, whole genome shotgun sequence.
ACCESSION   DAOOQX010000287 DAOOQX010000000
VERSION     DAOOQX010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54319_length_2388_cov_2.477068"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..378)
                     /locus_tag="PLX53_05430"
     CDS             complement(91..378)
                     /locus_tag="PLX53_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HOY72558.1"
                     /translation="MITVVAIMAIGILVGYLLRQKRKLIGFLERLTTWAIYLLLFLLG
                     IAIGTNEEIIRNLPTLGGRAFIISVAGVLGSILLSWATYRLFFKKDEEKQE"
     gene            complement(620..1624)
                     /gene="gap"
                     /locus_tag="PLX53_05435"
     CDS             complement(620..1624)
                     /gene="gap"
                     /locus_tag="PLX53_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOY72559.1"
                     /translation="MGKIKVAINGFGRIGRNVFKLALERPELEIVGINDLTDTKTLAY
                     LLKYDSTQGRFEGKVEFDAENLIVNGKKYKISAERSPLNIKWMETPDVVVESTGIFTK
                     RESEKGGYGDHIKNGAKKVVLTVPAKDEIDRMVVVGVNEDTLKPTDQCISNASCTTNC
                     LAPVVKVLNDSFGIERGYMTTIHSYTNDQRILDAPHSDLRRARSAAVSQIPTTTGAAK
                     AVGKVIPELKGKLDGISVRVPTPTGSLVDFVAILKKEVTKEDINAAMKKAAEGPMKGV
                     LEYTEDPIVSADIIHNPHSSIFDAQSTMVNGNMVKVLSWYDNEWGYSARVVDLIPKLF
                     "
     gene            complement(1920..>2388)
                     /locus_tag="PLX53_05440"
     CDS             complement(1920..>2388)
                     /locus_tag="PLX53_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY72560.1"
                     /translation="AAEEATYLAGLCRKVYLIVRKSYLRASKAMQEKVANTPNIEILF
                     EHTTKEIVGDQSGVNGVILTNTKTGKNVKVDISGFFLAIGHHPNSELFKPWLDTDEVG
                     YIKTLPDSTKTNLPGVFACGDVRDPHYRQAITAAGSGCMAAIDAERYLGERKF"
BASE COUNT          619 a          545 c          518 g          706 t
ORIGIN      
        1 ggtcggtagc agtcaaccat tggggaagca tgtccttaaa accaagcagt atgcctgctg
       61 caaaaaaagc cagtatggta agactacccc tcattcttgc ttctcctcat cctttttgaa
      121 gaacaggcgg taggtcgccc acgaaagtag aatgctgccc agaacgcccg caacgctgat
      181 tatgaacgcc ctgccaccca gcgttggcag gtttcttatg atttcctcgt tagttccaat
      241 tgctattcct agtaagaaaa gcagcaggta tatggcccac gttgtaagcc tttcgagaaa
      301 tccaatcagc tttcttttct gacgcaggag atacccaaca agtatcccaa tggccattat
      361 tgctacaact gtaatcattt gctatgaaac tttaaatcta ctttccgact atacattttg
      421 aagcaccatt cacttgcaag cgtaaaatat tgggtggggt ataaaatcga aaagtactct
      481 ttttttagtc tgaatttggc ttatgaattc agatgttgaa tttattctcc aaccatgaaa
      541 tgcaatcaaa taggtgtact ttacaaaatt aaaaaaggct acccaatggc gggtagccct
      601 tcattatcgc taggtatggc taaaatagct ttggaatcaa atctacaact ctggctgagt
      661 agccccattc gttatcatac caagaaagca cctttaccat gttgccgttg accatggtgc
      721 tttgagcatc aaagatggag gagtgggggt tatgaataat atcagctgaa acaatggggt
      781 cctcggtgta ctcaagaacg cccttcattg gtccttcggc agctttcttc atggctgcgt
      841 taatatcctc cttggttacc tcttttttca aaatggccac aaagtcaacc agcgaaccgg
      901 ttggggttgg gacgcgaacg gatattccgt caagcttacc tttcaactca gggataacct
      961 tgccaacagc cttggcagca ccggtggttg ttggaatttg ggaaacggct gccgaacggg
     1021 ctctgcgaag gtcgctgtga ggtgcatcaa gaatcctctg gtcgttggtg taggagtgaa
     1081 tggtggtcat gtaaccccgt tcaattccga aactgtcatt taaaaccttc accactggag
     1141 ccaggcagtt ggtggtgcag ctggcgttgg aaatgcactg gtcggttggt ttaagagtat
     1201 cctcattcac gccaaccact accattcggt caatttcgtc cttggcgggg actgttaaaa
     1261 caaccttttt ggcgccattt ttaatatggt cgccgtatcc acccttttcg ctctcacgct
     1321 tggtgaaaat cccggttgat tcaacaacaa catcaggtgt ttccatccac ttaatgttta
     1381 gtggagagcg ctcggctgat attttatact ttttcccgtt gacaatcagg ttctcggcgt
     1441 caaactcaac cttgccttca aagcgccctt gtgtagagtc gtactttagt aggtaggcca
     1501 gggttttggt gtcggtcaaa tcgttaatac caacaatttc aagttcggga cgttccagtg
     1561 cgagtttaaa cacgttacgg ccaattcgcc cgaatccgtt gattgctact ttaatttttc
     1621 ccatatctac ttcgtattag ttgattatgt aaatgttaac ccttttcgtg aagcaaagat
     1681 aagctaaaat tacatttacc gccccttttt tcaacttgtc aaatcaccta atttgcatac
     1741 cacttataac agcatgctct accaatttgt attcaaccgt ttgcgatgaa attcaaaatt
     1801 ttgccgcaat tgctaaaaaa tattaataat ttgatggttt ctttgtttag gtagtttttg
     1861 tagagggtgt tttaacccag acaaggacag ttttagattt tatgccgaat aaaaccaaac
     1921 taaaatttcc tctcacccag gtaccgttcg gcatcaatag ccgccatgca tcccgagccg
     1981 gcagctgtaa tggcttggcg gtagtgtgga tccctaacgt cgccacaggc aaatacacca
     2041 ggcagatttg ttttggttga atcgggcagg gttttgatgt agcccacctc gtcagtatca
     2101 agccatggct taaagagttc ggagttggga tggtggccaa tggccaggaa aaatcccgaa
     2161 atatcaacct ttacattctt tcctgttttg gtattagtaa gaatcacccc gtttaccccc
     2221 gactggtcgc ctacaatctc cttggtggtg tgttcaaaaa gaatttcgat gtttggagtg
     2281 ttggcaactt tttcctgcat tgcctttgat gcgcgcaggt atgatttacg aacaatcagg
     2341 taaaccttac ggcataagcc ggcaagataa gttgcctctt cggctgca
//
LOCUS       DAOOQX010000288         2371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_54843_length_2371_cov_2.985320, whole genome shotgun sequence.
ACCESSION   DAOOQX010000288 DAOOQX010000000
VERSION     DAOOQX010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54843_length_2371_cov_2.985320"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PLX53_05445"
     CDS             complement(<1..475)
                     /locus_tag="PLX53_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HOY72561.1"
                     /translation="MELQTIKQRFGIIGNAPGLNRAIDIARQVAPTDLSVLITGESGT
                     GKEVFPQIIHQFSARKHGNYIAVNCGAIPEGTIDSELFGHEKGSFTGATDSRQGYFEV
                     ANGGTIFLDEVAELPHSTQVRLLRVLETGEFIRVGSSKVIKTNVRVVAATNVNLYE"
     gene            complement(689..2146)
                     /locus_tag="PLX53_05450"
     CDS             complement(689..2146)
                     /locus_tag="PLX53_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOY72562.1"
                     /translation="MKPKPATTFQRRLNLFDSTAIVVGSMIGSGIFLVSADMARNLGS
                     PGWLLVAWAITGILTVIAALSYGELAVMMPHAGGQYVYLREAYNPLAGFLFGWTLFLV
                     IQTGSIAAVSVAFSKFLGVIFPWFSDNNVLLQVGSLKITTIHLMSIFIILFLTWVNTR
                     GINIGKYVQDTFSYTKIAALLLFFIVGFFIARNDEAVRLNREIFWQGQQLVDGKPVPL
                     SGISLIIALGIAMVGSLFAADAWNNVTFASEEIKDPKRTIPLSMVLGVVIVIVLYMLV
                     NYIYIKVLPVRGSPDATTVMGQGMQYASNDRVGTAAMYGTFGESAAIIMAILIMISTF
                     GCVNGMILSGARVYYAMAKDGLFFKQVGILNSMGVPGVSLALQCAWACILCLSGTYSN
                     LLDYVIFSVLIFYVLSVVGVFILRVKKPDVARPYKTWGYPVTPAVYILACTFIMVILL
                     IYKPAYTWPGLIIVILGIPVYYLWKKPKFSRTKRT"
BASE COUNT          703 a          561 c          503 g          604 t
ORIGIN      
        1 cttcatagag gttgacgttg gtggcggcca ccacccttac gtttgttttt atcaccttag
       61 aggagccaac ccttatgaac tcaccagttt caagaaccct taacagcctt acctgggttg
      121 agtggggaag ttctgccacc tcgtcgagaa aaatggtacc cccattagcc acctcaaagt
      181 aaccctggcg gctgtcggta gcccctgtaa acgaaccctt ttcgtgccca aacagctcgg
      241 agtcaatagt accctcggga atagcaccac agttaacggc tatgtagttg ccatgcttgc
      301 gtgcgctaaa ctggtgtatt atctgaggaa aaacttcctt ccctgtgccg ctctcaccgg
      361 taataagcac cgacaggtcg gtaggagcaa cctggcgggc tatgtcaata gccctgttta
      421 atccaggggc attcccaatt attccaaagc gttgttttat ggtttgtagc tccatgacat
      481 ttccctaaca cctgacaaaa ttacatattt ttctcaatct tgtattaatg ccttaggata
      541 aaaaatgccg ttgattttaa ctttcgaaca gggaggttac agcatgaaca ctttttagcc
      601 acacagccag ctgtctagac gcgctgattt ttaaaaatgt agcattttct tttacggaaa
      661 tttcaacatg ggcttgtatt gcaaccgctt aggtgcgttt agttctagaa aatttgggct
      721 ttttccaaag gtagtaaaca gggattccca aaataactat gataaggcct ggccatgtgt
      781 aggcaggttt atagataagc aaaattacca taatgaatgt acaggcaaga atatatactg
      841 ccggtgtaac tgggtagccc cacgttttat aaggacgagc tacgtcaggt ttttttaccc
      901 ttaagatgaa aacccccacc acggaaagca cgtagaaaat cagcactgag aatatcacat
      961 aatccagcag gttgctgtaa gtgcccgaaa ggcacagaat acaagcccaa gcacactgaa
     1021 gcgccagact gacaccaggt acacccatag aattaagtat gcctacctgt ttaaagaaca
     1081 ggccatcctt ggccatggcg tagtaaacgc gggccccgga tagtatcatc ccgttaacac
     1141 atccaaaggt tgaaatcatg atgagaatgg ccatgatgat ggctgcagat tcgccaaatg
     1201 ttccatacat agctgctgtg cctaccctgt cgttcgaggc gtactgcatt ccctgtccca
     1261 tcaccgttgt ggcatcgggc gatccacgaa cgggtaaaac cttaatgtat atgtagttta
     1321 ccagcatgta gagcactatg acaataacaa cgccaagcac catacttaac gggatggttc
     1381 gtttggggtc cttaatctcc tccgaggcaa aggttacatt attccatgcg tcggcggcaa
     1441 acaacgatcc aaccatggct atacctaaag caataataag cgaaatgccc gaaaggggaa
     1501 caggtttacc atcaaccagc tgctgccctt gccagaatat ctccctgttg agccttacgg
     1561 cttcatcatt tctggcaata aaaaaaccaa caatgaaaaa tagaagcaat gctgcaattt
     1621 ttgtgtagct gaatgtatcc tgaacatatt ttccaatgtt tattcccctg gtgtttaccc
     1681 aggtaaggaa gagtataatg aaaatagaca ttaggtggat tgtagtgatt ttcaagctgc
     1741 caacctgcag caaaacattg ttatcggaaa accacggaaa tatcacccca agaaacttgc
     1801 taaacgcaac tgaaactgca gcaatggaac cggtttgtat tacgagaaag agcgtccagc
     1861 caaataggaa accagctaga ggattgtagg cttctcttag gtaaacatat tgtcctccgg
     1921 catggggcat catcacggcc aactcaccat agctgagtgc tgctataacg gtaagaattc
     1981 cggttattgc ccacgcaacc agcaaccacc cgggcgagcc cagatttctg gccatatcgg
     2041 cgctaaccag aaatatgcca gagccaatca tgctacccac cactattgct gtggaatcaa
     2101 ataagtttaa tcgccgttga aaagtggttg ctggctttgg cttcattcta atctcaataa
     2161 gctacttctt aggggttgcc tgtaaacaga tgcaagaaaa ttaaaattat tctgattagc
     2221 aataccaaac cccatgaaac ggtatgctcc attcaaaaaa aatgcttcaa ccaaaacctt
     2281 tattggtgtc tgcatacaac cctgtaatta ggagcaacaa gcgactatta tggaatggat
     2341 agactgccag gtagaagtcc gttaagtatg c
//
LOCUS       DAOOQX010000289         2358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_55304_length_2358_cov_2.781155, whole genome shotgun sequence.
ACCESSION   DAOOQX010000289 DAOOQX010000000
VERSION     DAOOQX010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55304_length_2358_cov_2.781155"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1832
                     /locus_tag="PLX53_05455"
     CDS             <1..1832
                     /locus_tag="PLX53_05455"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOY72563.1"
                     /translation="AAEFAKELEEYGRIYMYRYRPGYKIFARTINDFPHKSLQAASIM
                     LMLSNNLDTAVAQHPHELIIYGGNGAIFQNWAQYRLTMKYLAEMTDHQTLVMYSGHPM
                     GLFPSFPDAPRVVITNGMMVPNYSSKDDWERFNALGVTQYGQMTAGSYMYIGPQGIVH
                     GTTITVINAGRMHSKPGEKALGGKVFVSSGLGGMSGAQPKAAVIAGVVGVIAEVNPKA
                     VEKRYQQGWVNEVYTDLEKLIPRIREASARKEAVSLAYQGNVVDLWERLAREDLRVDL
                     GSDQTSLHNPFAGGYYPAGLSFEESNRMMVDNPELFRQKVYESLRRQVKAISALTERG
                     MYFFDYGNAFLLESSRAGADVLKPNGEFKYPSYVQDIMGPLYFDYGFGPFRWVCTSSN
                     PSDLAETDAIALEVLENIASHAPEEIQQQLHDNIHWIREAGRNNLVVGSQARILYADS
                     EGRLRIAEAFNKAIREGRISAPVVLGRDHHDVSGTDSPYRETSNIYDGSRYTADMAIQ
                     NVIGDSFRGATWVSIHNGGGVGWGEAINGGFGLTIDGSADAERRLKMMLHWDVNNGIA
                     RRSWARNHGAISSIKREMERTPLLKVTLPNMADDNLIDDVVKG"
     gene            1868..2335
                     /locus_tag="PLX53_05460"
     CDS             1868..2335
                     /locus_tag="PLX53_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72564.1"
                     /translation="MQKFIRETGVLIAILVLCGSSCDELDSTNTITGVWRSREIYPGN
                     SYRTYNVSMEHYDQLDSTSYIIYNLYNLGLEVETYVQLNDTIFNILGCNSDSYIISGK
                     GYFHPLSFTIDWEYSVSGIDNDPFVMAHFEKPYNRGLSLVLFCRHNNICAREY"
BASE COUNT          607 a          579 c          605 g          567 t
ORIGIN      
        1 tagctgccga atttgccaaa gagctcgagg agtatggaag aatttacatg taccgttacc
       61 gtcctggcta taaaattttc gcccggacaa taaacgattt tccccacaaa tcgctgcagg
      121 cagcctctat aatgttgatg ctgagcaata atctcgacac agctgttgcc caacaccccc
      181 atgagcttat catctacgga ggtaatggcg ccatttttca aaattgggca cagtacaggt
      241 taaccatgaa gtacttggcc gaaatgaccg accaccaaac ccttgtgatg tactcaggac
      301 atccaatggg acttttccca tcatttccag atgcccccag ggtggtaatt accaacggga
      361 tgatggttcc caactactcc tctaaggacg actgggagcg ttttaacgct cttggcgtta
      421 cgcagtatgg gcaaatgacc gcaggttcct acatgtatat aggccctcag ggcattgttc
      481 acggcaccac cataacggtt atcaatgcag gacgaatgca ctctaaaccc ggcgaaaaag
      541 cattgggagg gaaggtattt gtatcctccg gattgggcgg aatgtctggg gcacagccta
      601 aagctgccgt tatagccggc gtggtggggg tgattgccga ggtcaaccca aaggcagtgg
      661 aaaaacggta ccaacaggga tgggtgaacg aggtttacac cgatttggaa aagctaattc
      721 cacgaattcg cgaggcctcg gcacggaagg aagcagtgtc gttggcctac cagggaaatg
      781 tggttgacct gtgggaaagg cttgccagag aagacctacg ggttgacctg ggtagcgacc
      841 aaacctcgct tcataaccca tttgccggag gctactatcc tgcggggcta tcctttgagg
      901 aatcgaaccg gatgatggtt gacaatcctg agctgtttag gcagaaggtt tacgaaagcc
      961 ttcgccgcca ggtgaaggcc atttcagccc tgacggaacg tggaatgtac ttcttcgatt
     1021 acggcaacgc cttcctgctt gagagtagca gggctggtgc cgacgtgctg aaacctaatg
     1081 gcgagtttaa atatccctcc tatgttcagg acataatggg tccgctgtac ttcgactatg
     1141 gctttggccc atttcgatgg gtttgcacct cttccaaccc ttccgacctt gccgaaaccg
     1201 atgctatagc cctggaggtt ctggagaata ttgcaagtca tgctccagag gagattcaac
     1261 agcaactcca cgacaacata cactggatta gggaagctgg taggaacaac ctggttgttg
     1321 gctcgcaggc cagaatactt tacgccgatt cggagggcag gcttagaatt gctgaagcct
     1381 tcaataaggc tatccgggag ggcagaattt ccgccccggt tgtgctcggg cgcgaccacc
     1441 acgatgtttc gggaaccgat tccccctacc gcgaaacctc caacatttac gatggatcac
     1501 gctacactgc cgatatggcc attcagaatg tgattggcga ttcattccgc ggggcaacgt
     1561 gggtttccat ccataacggc ggaggtgttg gatggggaga ggccattaac ggggggtttg
     1621 ggcttaccat tgatggttca gccgatgccg aacgacgcct taaaatgatg ctccactggg
     1681 atgtgaacaa cggaattgcc cgtagaagct gggctcgaaa ccacggtgca atttcatcca
     1741 taaaacggga aatggaacgc acacccctgt taaaggtaac acttcccaac atggctgatg
     1801 acaaccttat tgatgacgtg gtgaaggggt aagcgcccct tcaccctttc accttgatgc
     1861 aatggctatg cagaaattta ttcgggaaac aggcgtgttg attgccatct tggtcctatg
     1921 tggcagtagc tgcgacgagc tcgatagcac aaacaccatt accggcgtgt ggcggagtag
     1981 ggaaatatac cccggaaata gctacagaac ctacaacgtt agcatggagc actacgacca
     2041 gctcgactca acctcctaca taatttataa cctatataac ctgggccttg aggtggagac
     2101 ctatgttcag cttaacgata cgattttcaa catactgggc tgcaacagcg attcctacat
     2161 catttcagga aaggggtact ttcatcccct ttcctttacc attgactggg agtacagcgt
     2221 ttcgggcatt gacaacgatc ccttcgttat ggcacatttt gaaaaacctt ataacagggg
     2281 tttatcactt gttctttttt gtcgccataa taatatctgt gctcgagaat actgatgtaa
     2341 caagcagtag tttaactg
//
LOCUS       DAOOQX010000290         2358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_55305_length_2358_cov_2.779418, whole genome shotgun sequence.
ACCESSION   DAOOQX010000290 DAOOQX010000000
VERSION     DAOOQX010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55305_length_2358_cov_2.779418"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PLX53_05465"
     CDS             <1..249
                     /locus_tag="PLX53_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696795.1"
                     /note="catalyzes the zinc dependent deacetylation of
                     UDP-(3-O-acyl)-N-acetylglucosamine to
                     UDP-3-O-(3-hydroxytetradecanoyl)-glucosamine in the second
                     step of lipid A biosynthesis and catalyzes the dehydration
                     of beta-hydroxyacyl-ACP to trans-2-acyl-ACP in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine
                     deacetylase"
                     /protein_id="HOY72565.1"
                     /translation="AQVGGILVLNTVPDPENYTTYFLKIDQVRFKHKVVPGDTIIFRL
                     TLNEPIRRGIANMTGQAFVGNQLVMEGVLMAQIIKNPN"
     gene            249..1052
                     /gene="lpxA"
                     /locus_tag="PLX53_05470"
     CDS             249..1052
                     /gene="lpxA"
                     /locus_tag="PLX53_05470"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOY72566.1"
                     /translation="MAQLNAYIHPDAKIGKDVTISPFSYIAGDVIIGEGTWIGPNVTI
                     MDGARIGKNCRIFPGAVISAIPQDLKFKGEVTTAEIGDNTTMRECVTINRGTASKGKT
                     IVGSSCLLMAYSHVAHDCILKNNIILGNGTQLGGEVEIDDFAIVSAHSLVHQFEKIGA
                     HVIIQGGSKVNKDIPPYIIAAREPITFAGINLVGLRRRSFTHQQIADIQEIYRIIYNK
                     GLNVSQALAFIIDNIPQTAERDLIVNFIKNSKRGVLREYTGPDPNEGID"
     gene            1168..>2358
                     /locus_tag="PLX53_05475"
     CDS             1168..>2358
                     /locus_tag="PLX53_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOY72567.1"
                     /translation="MVLIYNLGIRIYLLLIWLVSPVNPKAKSWLAGRRGLLDEVELLA
                     QNNDPKIWVHCSSLGEFEQGRPLIERIRESYPTLKVVLTFFSPSGYKVRKNYSGADYI
                     FYLPLDTKRNAKRFVEAVKPKMAIFVKYEFWYHFLSELHAREIPTYLISANFRETQIF
                     FKPYGRWYRKFLSHFTHIFVQNHLSLELLNSVGIKEVTVTGDTRFDRVSRVASESATI
                     PLLNTFCDGEKVIVAGSTWPKDEELLLNYLSANADRVKMIIAPHEISPSRVGSIVSAS
                     KVEAICFTRPEGTSPKQARLLVLDTIGFLSSTYRYGCLAYIGGGFGAGIHNTLEAAVF
                     GIPVVFGPNYLKFQEAMDLIALGAGFSIKSQAELNDLLDRLLNDPTLLSQAGESAGAY
                     VKSGV"
BASE COUNT          642 a          512 c          538 g          666 t
ORIGIN      
        1 gctcaggtgg gtggcattct ggttcttaac actgttcccg accctgaaaa ctacaccacc
       61 tacttcttaa aaattgacca ggttcgtttt aaacacaagg ttgttcctgg tgatactata
      121 atttttcgct taaccttgaa tgaacccatc cgtagaggga ttgccaacat gaccggtcag
      181 gcattcgtcg gtaaccagct ggttatggag ggtgtgctaa tggctcaaat tattaaaaat
      241 ccaaattaat ggcacaactt aatgcataca ttcatcccga cgctaaaatt ggtaaggatg
      301 ttaccattag cccgtttagc tacatagcgg gagatgttat tataggtgag ggaacctgga
      361 ttggtccaaa tgttaccata atggatggcg cccgaatagg caaaaactgc cgaatttttc
      421 ctggagcggt aatttcagcc atccctcagg atttaaagtt taaaggcgag gtaacaactg
      481 ccgagattgg cgataacacc accatgcgcg aatgcgttac cattaacagg ggaactgctt
      541 caaagggaaa aaccattgtt gggagcagct gcctgcttat ggcatattca catgtagcgc
      601 atgactgcat actgaaaaac aacataatcc tcgggaatgg aacacagctg ggcggagagg
      661 ttgagattga cgattttgcc atagtgagcg cccattcatt ggttcatcag ttcgaaaaaa
      721 ttggtgccca tgttatcatc cagggcggtt cgaaggttaa caaggatatc cccccataca
      781 taattgctgc acgggagcct ataacctttg ccggtatcaa cctggttggg ttgcgtcgca
      841 gaagttttac ccatcagcaa atcgccgata ttcaggagat ttaccgtata atctacaata
      901 aagggttaaa tgtttcgcag gcacttgcct tcattattga caatatcccg caaacggccg
      961 aaagagatct aatagttaac ttcataaaga attctaaacg aggcgttctt agggagtaca
     1021 ccggtccgga ccctaacgaa ggaattgact aaatgatttt tcctctacta ccgggttttt
     1081 tatactgtta gattgaggat aggctatatt tcgaagcgtt ttatctttac ttaccctttt
     1141 tgcccctctt taattgtttg ctgttctatg gtgcttattt ataacttggg aatccgtatc
     1201 tacctgctac ttatatggtt ggtttcacca gtaaacccaa aggcaaaaag ttggctggcc
     1261 ggacgaaggg ggcttctcga tgaagttgaa ttgctggccc aaaataatga cccaaaaatt
     1321 tgggttcact gctcatcgtt gggtgagttt gagcaaggac gcccattaat agaacgcata
     1381 agggagagtt atcctacgct aaaagttgtg cttacctttt tttctccatc gggctataaa
     1441 gtgaggaaaa attatagcgg agccgactac attttttacc ttcctctcga tacaaaaagg
     1501 aacgccaaaa gatttgtcga ggctgtaaaa ccaaaaatgg ccatttttgt taagtatgag
     1561 ttttggtacc actttctctc cgagcttcat gccagggaaa ttccaacata ccttatttcg
     1621 gccaacttta gggaaaccca aatttttttc aagccctacg ggaggtggta ccgtaaattt
     1681 ttatcgcatt ttacccatat ttttgttcaa aaccaccttt cgctagagct gctcaactcc
     1741 gtgggtatta aggaggtaac tgttaccggt gatacccgtt tcgacagggt gtcgagagtt
     1801 gcctctgagt cagctacgat tccccttctc aatacctttt gcgatggtga aaaggtaatt
     1861 gtggccggga gtacctggcc caaggatgaa gagctactct tgaactacct atccgcaaat
     1921 gccgatagag ttaagatgat cattgctcca catgaaatta gcccgagcag agtgggttca
     1981 atagtgagcg cttcaaaggt tgaggccatt tgttttaccc ggcccgaagg aacttcgcca
     2041 aagcaggcac gactgttggt actcgataca ataggcttcc tctcttcaac ctaccggtat
     2101 ggctgcctgg catatattgg cggtggcttt ggcgctggta ttcacaacac cctggaggca
     2161 gcagtttttg gcataccagt agtatttggc cccaattacc tgaaatttca agaagcaatg
     2221 gatttaattg ctttaggagc gggtttttct atcaaatcgc aggctgaact caacgatttg
     2281 cttgacaggc ttctgaatga cccgacattg cttagccaag caggcgaaag tgctggtgcc
     2341 tatgtaaaat cgggtgta
//
LOCUS       DAOOQX010000291         2347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_55664_length_2347_cov_2.729058, whole genome shotgun sequence.
ACCESSION   DAOOQX010000291 DAOOQX010000000
VERSION     DAOOQX010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55664_length_2347_cov_2.729058"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..2100
                     /locus_tag="PLX53_05480"
     CDS             187..2100
                     /locus_tag="PLX53_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72568.1"
                     /translation="MGILSLKKSFLQLTLRLGLAITVHLSFLLFTSTSFALTTSKFDS
                     VQLFWNPDTLSRSDTNLFNRRGNEVLKSNNAIVNNLLDLIYCEEEDTRQKEIYAEQAK
                     AYQKFSRYAGRIIADIQIVRLKPFGEYINDTTLKPENSLQKFGNSLHSLTLRRSVENN
                     LLFSEGQELDPVDLVETEQLLRNLLYIEDVGINIEPLQDTNYVKATVVTKDKWTLNMG
                     VRIESSAKGRITLSERNFAGIGIGATGRVYYDEEQKEKWGYMGELNIPNIAGTFITSD
                     LWFREGVGYKSYCVDVKRDFYASKTKYALGGTYVKSQEPYKIFPVDSIVSIDYSLMSF
                     WVGRSFRVGKPVIIASPYIFTLALKYRQVDFYKSLPTSMNLNPYFHSNYQVLLAAGFS
                     NQNFAQDNFIYSFGATEDIPVGFIVQLIGGVEDSEYQRRLVAGGEISAAEITPAGYLF
                     IAARTGGYMAEGPRAEQGMINFKSTFISNKFTLGKYFGRQHVGVSFTRGFSRFEGERE
                     YLALNKDNGITGLRSNSLVGQTRLIVNFETTLFSPHLYYGFRPSCFAFCDLGMVGGSN
                     EVIFGNSLFSGFGLGFKVKNESLIFPSFIFRLGYYPNVPQNADVAYWIITSQDRKKFE
                     QFRMKEPALLPFE"
BASE COUNT          673 a          459 c          513 g          702 t
ORIGIN      
        1 gaacgctatc cgaaaaggga ggtgccgggt taggactaat tgatattgcc cgcaaaacag
       61 gcgaaccatt gaactaccaa ttccttcaac tcgatgagga taactatttt tttatactta
      121 aagtcgaaat taatgcccgt aagttctaca agtaagtggt taaaggagta atcctatgtc
      181 ttattaatgg gtattttgtc gcttaaaaaa agctttttac agctaacctt acgcttagga
      241 ttagcaataa cggttcattt atcatttctg cttttcacct ccacttcttt tgcattaaca
      301 accagcaagt ttgattctgt tcagctattt tggaatcccg acacccttag tagaagcgac
      361 accaacttgt tcaatcgtcg agggaatgag gttcttaagt ccaataatgc catagtaaat
      421 aacctgttag acctaattta ctgtgaggaa gaggatacta ggcaaaaaga aatttatgcc
      481 gaacaggcaa aggcatacca gaagttctca agatatgctg gaagaataat agccgatatt
      541 caaattgtac ggttaaagcc ttttggggag tacattaacg atactacact aaaacccgaa
      601 aacagccttc agaaatttgg gaactcgcta cactcgctta cattacgccg gtcggttgaa
      661 aacaacctgt tattttccga ggggcaagaa cttgacccgg ttgacttagt ggaaaccgaa
      721 cagctccttc gcaaccttct ctatattgaa gatgtaggta ttaacattga acccctgcag
      781 gacactaact atgtgaaagc tacggttgtt acgaaggata agtggactct gaacatgggg
      841 gtacggattg aaagttcagc gaagggacgg ataacccttt cggagagaaa ctttgccgga
      901 ataggtatag gggcaaccgg gcgtgtttac tacgacgagg aacaaaagga gaaatggggg
      961 tacatgggtg agctgaatat ccctaacatt gctgggactt tcattaccag cgacttgtgg
     1021 ttccgtgaag gcgtcggcta caaatcttac tgtgtagatg tgaagcgtga cttttacgcc
     1081 tcaaaaacca agtatgcact aggcggaacc tatgttaaaa gtcaagaacc ctacaaaatt
     1141 tttcctgttg attcaatagt ttcgatagac tatagcctaa tgagtttttg ggtaggccgt
     1201 tcatttaggg ttggtaaacc ggttataatt gcttcacctt acatttttac ccttgctcta
     1261 aagtaccggc aggttgattt ctacaaatcc ctaccaacat ctatgaacct aaacccgtac
     1321 tttcactcta actaccaagt tttgttagca gccggatttt ctaaccaaaa ttttgctcag
     1381 gataacttca tctactcttt tggcgcaacc gaggatattc cggttggatt tatagtgcag
     1441 cttattggag gggttgagga tagcgagtac cagcgacgat tggtggcagg tggtgaaatt
     1501 tcggctgccg agataacacc tgccggatac ctttttattg ctgctcgtac cggaggctac
     1561 atggccgaag gcccacgagc cgagcaagga atgatcaact tcaaatctac ttttatttct
     1621 aataaattta cccttggaaa atattttggc cggcaacatg ttggtgttag ctttacaagg
     1681 ggcttttcgc ggtttgaggg ggaaagggaa taccttgcac ttaacaagga taacgggatt
     1741 accggtctaa gaagcaattc ccttgtgggt caaacacgcc taatagttaa ttttgaaaca
     1801 actttgttta gcccacattt atactatggt tttagaccat cgtgttttgc cttctgtgat
     1861 ttgggaatgg tgggaggttc aaacgaagta atttttggca attctctttt tagcggtttc
     1921 ggtctgggtt ttaaggtgaa aaatgaaagt ttaatctttc cgtcatttat ttttcgtctt
     1981 ggttactacc ctaatgtccc ccaaaatgcc gatgttgcct attggattat tacctctcag
     2041 gatcgtaaga agtttgagca gttcaggatg aaagagcctg ccctacttcc cttcgagtag
     2101 cctggcatct gcgaacaatt ttcatttcac gttgtttacc atgaaaattt acaacccggc
     2161 ataattgaac atttctggtt aattgcatgg ttgtattaag tggcagctaa agtcattttt
     2221 gattttacat tattaaaata cctaaaaatt attgaactat gggagtacta gtaggacaga
     2281 aagcgcctgt tttcaaggct agcgcagtta taaagggtca cgaaattgtt gagaattttt
     2341 ctcttga
//
LOCUS       DAOOQX010000292         2341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_55854_length_2341_cov_3.456693, whole genome shotgun sequence.
ACCESSION   DAOOQX010000292 DAOOQX010000000
VERSION     DAOOQX010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55854_length_2341_cov_3.456693"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLX53_05485"
     CDS             complement(<1..349)
                     /locus_tag="PLX53_05485"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY72569.1"
                     /translation="MKRPGILNVWFWALFLCTSGNLMAQFTVSGTISDARTKAALPFS
                     NLLIQGTITGAVSDIEGKFTLEVTGNQEVTLIVSSVGYLTQMVKASPSNNTLDIQLRE
                     DATNLEEVIVTGLA"
     gene            complement(558..>2341)
                     /locus_tag="PLX53_05490"
     CDS             complement(558..>2341)
                     /locus_tag="PLX53_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY72570.1"
                     /translation="VSFECLETLVGTAIPSTTAGTSFTINPGATPGINLLRYVPLGTN
                     GEKGNITYIFVYVDRASCPMDSCNMVSNGGFESSSGCGGLYPQNLLANAPSIDCWESL
                     AASPDLFTRTCTSFGLQVGVSTFNSSPATDSHNGAPNDKFLGLYRSAAFPQDFSEAAQ
                     TKLATPLQPGKSYELSFWAKTNNNINGPNSNIPALVEFASDVSYPLIHTLKYLPGVNF
                     QTLFPNVSVPNDSVWHYFSQTFTFNGSTPHANLIVAYGALNIPQGVSGAYFFIDDIRL
                     KEVSCQSCTCGTHSDMSYRPYQGAQNIFVDCGDTLVAQCNGPIPWTFGGKFLCQGNSC
                     SDTAQMFWILNKPNGTVKTGPMLADPFFNFAIPAAEFNISGFYNLKLKGVCGSDTCYC
                     NFVVEVKCDSCCSDMNVFCQAVEEALNLIVDTCKVTLNIGNLRCNSYIESVDWGDGDQ
                     SIGHFGSGSILTHVYSTSGTYYISYVAIGQDEQGFDCIEKTIYDTIVVCNRMSTSNKD
                     LKATGSFHIFPNPSSGRLTLQTKGIQLKAAQFQILDLWGRKIYTGKLPNGQPNYELSI
                     ASLPPGIYFVKLLDRGELLWVHKVIKQ"
BASE COUNT          666 a          489 c          561 g          625 t
ORIGIN      
        1 aagccaaacc agtaacgatg acttcctcca ggttggtggc atcttcgcgg agctggatgt
       61 ccaacgtgtt gttggaagga ctcgccttga ccatttgagt gaggtagcca actgaagaga
      121 caatcaacgt aacttcttga ttaccggtaa cttcaagggt aaacttgccc tcaatgtctg
      181 aaactgctcc cgtgatggtt ccctggatga ggaggttgga gaaggggagt gctgctttgg
      241 ttcgggcatc cgagatggta cccgaaaccg tgaattgggc catcagattt ccgctggtac
      301 atagaaatag cgcccaaaac catacgttaa gtattcctgg tcttttcata ctaatggatt
      361 atttgttatc aagaataaaa agcgaaataa aagtaatgct attgttttta atggtaaagc
      421 aacatatcta ttctttttgg aaaaattggc aaaatgtcga aaatgggcaa ttgtgtggat
      481 tttagctccg gtgctgtttt ttttcaaaaa aaaatgcttc ccggcagaag cagtacactc
      541 atctgccggg aaagcattta ttgtttgatt actttgtgta cccagagcaa ttcgccacgg
      601 tcgagcaact ttacaaaata aatcccgggt ggcaatgagg caattgataa ctcataatta
      661 ggctgcccgt tggggagctt tccggtataa atttttctgc cccaaaggtc aaggatttgg
      721 aattgtgcgg cttttaactg gattcctttc gtttgtaaag tgagccgacc tgagcttggg
      781 ttggggaaga tgtggaagct tccggttgct ttcaggtctt tattcgaagt agacatgcga
      841 ttacaaacga caatggtatc gtagattgtt ttttcaatac aatcaaatcc ttgttcatcc
      901 tggcctattg ccacgtagga gatgtagtag gtgccactgg tcgaataaac atgcgtgagt
      961 attgaccctg aaccaaaatg accgatactc tgatccccat caccccaatc taccgattcg
     1021 atgtaactgt tacaacgaag attaccgatg ttcaaggtga ccttgcacgt atctacaatc
     1081 agattgaggg cttcctcaac ggcctgacaa aaaacattca tatcgctgca acaggaatcg
     1141 catttcactt ctactacaaa attacaataa caggtgtccg agccacaaac accctttaac
     1201 tttaaattgt aaaagccgga aatattgaat tcagcggcgg gaatggcaaa attaaaaaat
     1261 ggatcggcca acattggacc agtttttacg gtcccatttg gtttattgag tatccagaac
     1321 atctgggcgg tatcgctgca tgagttgccc tgacaaagga acttacctcc aaaggtccag
     1381 gggattgggc cattgcattg ggctacgagg gtatccccac aatcaacgaa gatattctga
     1441 gcgccctggt agggtcggta cgacatgtcg ctgtgggtgc cgcaggtgca gctttggcag
     1501 cttacctctt tcagcctgat gtcatctata aagaaatagg caccgctaac cccctgaggt
     1561 atgttcaagg caccgtaagc cacgataagg ttggcatgtg gagttgaacc attgaaggta
     1621 aaagtctgac tgaaataatg ccagacgctg tcgtttggaa cactaacatt ggggaagaga
     1681 gtttggaaat tgactcctgg caaatatttc agggtatgta tcaatggata agaaacatcc
     1741 gatgcaaact caacgagggc tggaatattg ctgttcggac cattgatgtt gttgttcgtt
     1801 ttggcccaaa aggacaactc ataagacttt cctggttgta aaggtgttgc cagtttagtt
     1861 tgtgcagctt cggaaaagtc ctgtgggaaa gcggcggacc ggtacaatcc taaaaactta
     1921 tcattgggcg ctccattatg ggaatcagta gctggactgc tattgaaggt ggaaacaccg
     1981 acttgcaaac caaaagaagt gcatgtcctg gtaaataaat ccggcgaagc agccagggac
     2041 tcccagcagt caatactggg cgcatttgct aagaggtttt gagggtataa tccaccacat
     2101 ccagagcttg attcaaagcc accattcgat accatattac aagaatccat cgggcaactt
     2161 gctcgatcaa catagacaaa tatataggtg atatttcctt tttcaccatt ggtacctaag
     2221 ggtacatagc gcaagaggtt gatccccgga gtagcacctg ggttaatagt aaaggatgtt
     2281 ccggcagtgg tgctggggat agcggtacca accaatgttt ccaaacattc aaaggataca
     2341 g
//
LOCUS       DAOOQX010000293         2340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_55899_length_2340_cov_2.513348, whole genome shotgun sequence.
ACCESSION   DAOOQX010000293 DAOOQX010000000
VERSION     DAOOQX010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55899_length_2340_cov_2.513348"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            365..>1739
                     /locus_tag="PLX53_05495"
     CDS             365..>1739
                     /locus_tag="PLX53_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HOY72571.1"
                     /translation="MKLHSNFAILLVATAMMACNSPKDRVEVWVSKADSTILLEQQPG
                     INFSNNQIGEVIEVDTNQKCQMINGVGAALTWSSAYVFKNYLTPETRLQLFRELFTPE
                     GIGINVVRLTIGSSDFSRGNHSYSETPDTSLSNFSLAMDMGEVVPMMKEILAVNPEIT
                     IIASPWSPPAWMKSSNSMVGGNLLKQHYGAYANYLVKYIKAMQEQGIPIYGITVQNEP
                     EFGTATYPCMLMSAQEQNEFIRYYLGPRMAQNGLTTRIILFDHNCDSPQYPISILNDS
                     ITRKYVDGSGFHLYKGEIEALCTVHEAHPDKNIYFTEQSGGGWAPNFGDNLLWYSETL
                     MVKAMRCWSKTVLLWNLALDENDGPKNNGCSNCWGVVQVPSNGAVKRNAEYYALGHFG
                     KFVLPNATRLVSTMQKVSNVAFLNPDGSKVLIAVNTSGQPVAFTVKCGGRQFIYQMGG
                     REVVTFRW"
     assembly_gap    1740..1749
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1899..>2340)
                     /locus_tag="PLX53_05500"
     CDS             complement(1899..>2340)
                     /locus_tag="PLX53_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72572.1"
                     /translation="EEFRRIYPVKTICMHGSPKSVLDNRDIWKEYDYRQLGIIGEPYF
                     DIDFDKFFYLTDTGRCWNGYKYSVRDKMPQQERWVKEGLVFKTTNDIIKAAQDNKLPN
                     QIMITVHPQRWTNKPALWLWELAAQRAKNVFKAFRISSLARQQA"
BASE COUNT          648 a          534 c          537 g          611 t
ORIGIN      
        1 tacagaaaca aggttacgtg gcgaatatta accacaaagt tctcaaagaa tgcacgaaga
       61 acacagagaa aaaatgaaag agtatgatga actctaatgt tatttcatta aaggatggga
      121 ttaaaagggt tataaatggc actttgtgaa ctttgtgtag catcctggtg ccctttgtgg
      181 taaaatataa ttttgcatct ttgaatgcaa cctggtatta agtcggtaaa gagaataaaa
      241 tttgcaccta acggattagc ttttgccgga attaataaac caacaaaaaa ctgctggaga
      301 aaatgaaaat atctattacc taaaaattta gggttttagt taaacttaca aaaaaataat
      361 cactatgaag ttgcattcga attttgccat cttgctggtt gcaaccgcca tgatggcttg
      421 taactccccg aaagataggg ttgaagtctg ggtttcaaaa gccgattcta ccatactact
      481 agaacagcag cccggcatta atttttccaa caaccagatt ggtgaagtta ttgaggttga
      541 tacaaatcaa aagtgccaga tgattaatgg cgtaggtgcg gctcttacct ggtcatcggc
      601 ctacgttttt aagaattacc tcactcctga aacccgcctg cagctttttc gcgagctgtt
      661 tacccctgag ggtattggca tcaacgtggt taggctaact attggctcct ctgatttttc
      721 acgaggaaac cacagctaca gcgaaacgcc cgacacctca ctttcaaact tcagcctggc
      781 aatggatatg ggcgaggttg tgcccatgat gaaagaaatt ttggcggtaa atcccgaaat
      841 tacaattatt gcctcgccgt ggagcccacc ggcatggatg aaatcatcca acagcatggt
      901 aggagggaat ctacttaagc agcactatgg tgcttatgcc aactacctgg taaagtacat
      961 caaagccatg caggagcaag gaattcccat ttacggaata actgttcaaa atgagccgga
     1021 gtttggcacg gccacctacc cctgcatgct tatgagcgct caggagcaga atgagtttat
     1081 tcgctactac ctgggacccc ggatggcaca gaatggcctg acaacccgaa taatactatt
     1141 tgaccataac tgcgactccc cgcagtatcc cattagcatt ctgaacgatt ccattactcg
     1201 gaagtatgtg gatggttcgg gttttcacct ctacaaaggc gaaattgaag ccctttgcac
     1261 agttcacgag gcccatcccg ataagaatat ctacttcacc gaacagtcgg gtgggggatg
     1321 ggcgccaaac ttcggcgaca acctgctctg gtactccgaa acccttatgg tgaaagctat
     1381 gcggtgctgg agcaaaaccg ttttgctatg gaatcttgcc cttgatgaaa atgatggccc
     1441 caagaacaac ggttgctcca actgctgggg ggttgttcag gtgccctcca acggagccgt
     1501 taagaggaat gccgagtact atgccctggg ccatttcgga aagtttgttc tacccaacgc
     1561 cacacggctg gtctccacca tgcaaaaggt atccaacgtg gctttcctga accccgatgg
     1621 ttcaaaggtg ctgattgcgg tgaatacatc cggacaacct gttgccttta ccgtgaagtg
     1681 cggcggcagg cagtttatct accagatggg tggacgtgag gtggttacgt tccgctggan
     1741 nnnnnnnnnt tatcaggtgc agtgtgtggc catgcctgaa tactcgccca aaccccagct
     1801 agaatgtcag tgtttacgcg agtattttaa cgataaaaag taaaccgcac gatggggttt
     1861 tcgtgcggca gtgtaggaat gcaggcaata tccggcggct acgcttgttg ccgggcaagt
     1921 gagcttatcc tgaaagcttt aaacacattc ttagcccgtt gggcggcgag ttcccataac
     1981 cacaatgccg gtttgttggt ccagcgctgg gggtgaacgg taatcataat ctggttgggg
     2041 agtttgttat cctgcgcagc ttttataatg tcgttggtag ttttaaaaac caatccttct
     2101 tttacccagc gctcctgctg cggcatcttg tcgcgtacgg agtacttata gccattccaa
     2161 caacgcccgg tatcggttaa gtaaaaaaac ttgtcgaaat caatatcaaa gtacggctca
     2221 ccaataatcc ctaattgcct ataatcatat tccttccaaa tatccctatt atcaagtacc
     2281 gatttcgggc taccgtgcat acaaatagtt tttacaggat atatcctccg aaactcctct
//
LOCUS       DAOOQX010000294         2338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_55955_length_2338_cov_3.338590, whole genome shotgun sequence.
ACCESSION   DAOOQX010000294 DAOOQX010000000
VERSION     DAOOQX010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55955_length_2338_cov_3.338590"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLX53_05505"
     CDS             complement(<1..615)
                     /locus_tag="PLX53_05505"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOY72573.1"
                     /translation="MCPQGQKTGSFRINKNFELLRSRQGFVVLLACTLILGIPVASAQ
                     KAFGPGEKLNYKIYYGLIHGGEAVLEVRKGDYKGKPANHIYMAGKTVGLANKFYYVED
                     IYESYTDPETDWPYFSIRNIHENRYRHYSTQVWDHWSRKDSSIVNSSKTGEVVVVKGC
                     QDILSSFYYLRNILIKSDPVKDQLFIVDTYFTDEKFPLMVRFKGR"
     gene            complement(575..1282)
                     /gene="tsaB"
                     /locus_tag="PLX53_05510"
     CDS             complement(575..1282)
                     /gene="tsaB"
                     /locus_tag="PLX53_05510"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOY72574.1"
                     /translation="MATILCMETGSTTCSVALGSESGVIGFREVSDANIHASALPVLI
                     EELIRETGFELGNIDAIAVSMGPGSYTGLRIGVSLAKGMCYALHKPLIAVGSLEAMVT
                     GVGEFINMESINNNSLLSPMIDARRMEVYTQLFNRKGEMLSEVKALVVETDTFSDLLK
                     KNTVVFFGSGASKCQQVIGNSGAIFVEHFKPSARYLLPLAQKKFRDELFEDIAYFEPY
                     YLKDFVPTRPKNRIIPD"
     gene            complement(1540..>2338)
                     /locus_tag="PLX53_05515"
     CDS             complement(1540..>2338)
                     /locus_tag="PLX53_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOY72575.1"
                     /translation="KEAEENLRKTSIYSPISGTVSKLNVELGERVLGTIQMAGTELMR
                     IANLNRMEARVDVNENDIVKVNIGDTALVEVDAYLDRKFKGIVTQIANTANVQGVSTD
                     QVTNFEVRIFLLDDSYKDLVTDSRLSPFRPGMSTTVEILTETRSNVLTVPILAVTVRS
                     DSTAKKDITGMEKVDDSSTPIEQSNSKLNKPKEIVFAVSGDTARIITVKTGIQNNQYI
                     EIVEGLKGDEDVVTAPYSAISRKLKDKSLVKKVKTKNDLLKAEVAKK"
BASE COUNT          562 a          569 c          529 g          678 t
ORIGIN      
        1 ccgaccctta aagcgaacca ttagcggaaa tttttcatcg gtaaagtagg tgtcaacgat
       61 aaagagctga tccttcacag gatctgattt tatcaggata ttacgaaggt agtagaacga
      121 cgagagtata tcctgacatc cctttaccac taccacttct cctgttttgg agctgttgac
      181 tatgcttgag tccttacggc tccagtgatc ccaaacctgg gtgctgtagt gccggtaacg
      241 gttctcgtga atattcctaa tcgagaagta tggccagtcg gtttcggggt cggtgtagct
      301 ctcgtagata tcctcaacgt agtaaaactt gttggccagg ccaaccgttt tgccggccat
      361 gtaaatatga ttggcaggct ttcccttgta gtcgcccttc ctcacctcga gcacagcctc
      421 gccgccatgt ataagcccgt agtaaatttt gtagtttaac ttttcacccg gtccaaatgc
      481 cttttgagcc gaagctaccg gtatgcctag aattagagtg caggccagca gtacaacgaa
      541 tccttggcgg gatcgaagaa gttcaaaatt cttgttaatc cggaatgatc ctgttttttg
      601 gccttgtggg cacaaagtcc ttaaggtagt atggctcaaa atatgcaatg tcctcaaaca
      661 attcatctct aaatttcttt tgtgctaaag gtagtaaata gcgagctgat ggtttgaaat
      721 gttcaacaaa tattgcacca ctgttaccaa tcacctgttg acacttggat gctccgctcc
      781 cgaaaaatac gacagtattt tttttaagca aatcgctgaa agtatccgtt tccaccacca
      841 aggcttttac ctccgaaagc atttctccct tcctgttaaa aagttgggtg taaacctcca
      901 ttcgtcgggc atctatcatg gggctgagca gagagttgtt gttgatgctt tccatgttga
      961 taaattctcc aaccccggtt accattgcct ccaggctacc cacagcgatt agaggcttat
     1021 gcagagcata gcacattccc ttggcaagcg aaacacctat acgcaggccg gtgtaggagc
     1081 caggacccat gctaacagca atggcatcaa tattccctaa ttcaaaaccc gtttccctta
     1141 tcagctcttc aatcagaaca ggaagcgccg aggcatggat attggcatcg gaaacctctc
     1201 tgaatccaat aacgccgctc tcgctgccca gtgctaccga gcaggttgtt gagccggttt
     1261 ccatgcaaag aatcgttgcc atgttggtgt tgcaattgtg cgaagctaat tcttcagggt
     1321 tgctacagca attttttgtc tgcaaccctg gcaattccta ttttttcatt tcaggttgat
     1381 gagggcctgt tggcttaacg caaaaacgtt gggtttcagg ggtgaaaagc ccattaaacc
     1441 ctaccacctg tttgctgagc catccacaac ttactgccaa gccaagcctg gcagcagatt
     1501 gggtagatac cgggttagga ataacggtca acccgaatgc tatttctttg caacctcagc
     1561 tttcaggaga tcatttttgg tcttaacctt tttaaccagc gatttgtcct ttagcttgcg
     1621 tgagattgcg ctgtagggag ccgtaaccac atcctcatca ccctttaatc cctcaacaat
     1681 ttcgatgtac tggttgttct gtattccggt tttcaccgtg ataatcctgg ctgtatcgcc
     1741 agaaacagca aatacaattt ctttaggttt gttcagcttc gagttggatt gttcgatggg
     1801 ggttgacgaa tcgtcaacct tttccattcc ggtgatatcc ttcttggcgg ttgaatcgct
     1861 tcgcactgta accgctagta tgggtacggt gagaacgttg ctacgggtct cggtaagaat
     1921 ttcaacggtg gttgacatgc ccgggcggaa agggctaagc cgggaatcgg taaccaggtc
     1981 tttatatgag tcatccagga ggaatattct aacctcaaag ttggtaacct ggtcggttga
     2041 gaccccctga acatttgcag tgttggcgat ttgggtaact atccctttaa acttgcgatc
     2101 caggtatgca tcaacctcca ccagagcggt gtcgccaata ttcaccttta caatatcatt
     2161 ctcgttaaca tcaaccctgg cctccatccg gtttaggttg gcaatccgca tgagttcggt
     2221 tcctgccatt tgaatggttc ccagcacccg ttcgcccagc tctacgttta gcttcgatac
     2281 ggtgcccgat atgggtgagt atatggatgt tttgcggagg ttttcctcgg cttcctta
//
LOCUS       DAOOQX010000295         2331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_56192_length_2331_cov_3.231547, whole genome shotgun sequence.
ACCESSION   DAOOQX010000295 DAOOQX010000000
VERSION     DAOOQX010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56192_length_2331_cov_3.231547"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1737)
                     /locus_tag="PLX53_05520"
     CDS             complement(199..1737)
                     /locus_tag="PLX53_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOY72576.1"
                     /translation="MLTKLDLKQLEERGITPQQVEGYMRMFREGFPFLPIERPATVGD
                     GIISLEDDEVDDLVEMFKDFQGSKVKFVPASGAATRMFKHLFEFRDEYPVKGLECFND
                     KGFNSAYSFFQNIKRFPFYDKLYNELWSVGYRLESLLEKKDYLPVVNALLDSNGLNYG
                     SLPKALIHFHRYGEIARTALEEHLVEAAKYARDQHGVANLHFTVSPEHLKGFQELVER
                     VKSFYENHYEVTLNVAYSVQKPTTDTLAVDLENNPFRNPDGSLHFRPGGHGALIENLN
                     ELNEDIIFIKNIDNVTPDHLKAATIRYKKALAGLLVSYKNLVNGYIERLLNEPVSEEQ
                     LALMLEFTTDELCILPPANLNRQNSDAVKKYLMEILDRPIRVCGMVKNQGEPGGGPFW
                     AKNPDGTTSLQIVEQSQVDMGNPRVKEIFGRSTHFNPVDIACSIRNYKGEKFNLSNFL
                     DPQAGFISIKSKNGKQLKALELPGLWNGAMSRWITFFVEVPIATFTPVKTVNDLLRSE
                     HQPE"
     gene            complement(1790..>2331)
                     /locus_tag="PLX53_05525"
     CDS             complement(1790..>2331)
                     /locus_tag="PLX53_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOY72577.1"
                     /translation="VWQEAVTLVTWPRGADGIFALRDFEFYDSNGNKLIAATSSWLVV
                     GVENRRPVRIAEWYLESDFASRSALNRNAAKIPESIVQPAFTEQFEVRAGDIDMNLHV
                     NNVRYIDWAYNTFTISSLRKYFPKKISVNFNSEAKEGDSILINRKNIPGNSTLININS
                     RLEGEKNYCRIQFDWRNLE"
BASE COUNT          657 a          538 c          492 g          644 t
ORIGIN      
        1 attgttgaag cgcgggaccg cagcttagca ggaacgtctg cacctgctga ggggctatac
       61 ctcacagaag ttaagtaccc caaagaaatg tttttgtgag cttacggaga agaattctgt
      121 aaaaacgatt aatattagcc aaagcattcc ttcaaataaa aacttcagtc aaaatgattt
      181 aggagcaact ttcccaagtt attcaggctg atgctcactt cgaagtaaat cgttaaccgt
      241 tttaaccggg gtaaaggtag caattggcac ctcaacaaag aaggttatcc atcggctcat
      301 ggctccattc cataggcctg gcagctcaag cgctttaagc tgcttgccgt ttttactttt
      361 aatcgaaata aagccagctt gaggatcaag aaaattagac aggttaaact tttcgccttt
      421 ataattcctt atgctgcaag caatatcaac cgggttgaag tgggttgacc tgccaaaaat
      481 ctcctttaca cggggattgc ccatgtcaac ctgcgactgt tcaacaatct gcagcgaggt
      541 ggttccatca ggattttttg cccagaacgg tccaccgcca ggctcgcctt ggtttttcac
      601 cataccgcaa acccggattg gccggtcaag aatttccata aggtattttt taacagcatc
      661 gctattttgc ctattgagat tagcaggggg caaaatgcaa agctcatcgg ttgtgaactc
      721 aagcattagg gcgagctgct cctcggaaac cggttcgttg agcaatcgtt cgatataacc
      781 attcaccaaa ttcttatagc tgacaagtaa tccagcaagc gcctttttgt agcgtattgt
      841 agctgcttta agatggtcgg gggttacatt atctatattt ttaatgaaga tgatatcctc
      901 gttcagctca ttcaaatttt cgatgagcgc accatgccca ccggggcgga agtgaagtga
      961 accatcggga ttgcgaaagg ggttattctc caagtcaact gcaagagtat cggtagtcgg
     1021 tttttgaacg gagtaggcca cgttaagggt aacctcgtag tggttttcgt agaatgactt
     1081 gacacgttca accagctcct gaaacccttt tagatgctct ggcgaaacgg taaaatgaag
     1141 gttggctact ccatgctggt ctcgggcgta cttggctgcc tcaaccaggt gctcctcaag
     1201 cgctgttcgg gcaatttcgc catagcggtg aaaatgaatc agcgcttttg gtaaagaacc
     1261 atagtttaaa ccgttggaat ccaataaggc gttaactaca ggcaggtaat cctttttttc
     1321 aagtaagctt tccagccggt aacctacgct ccaaagctcg ttataaagct tgtcgtaaaa
     1381 gggaaatcgt ttgatgtttt gaaagaagct gtatgcggag ttaaaaccct tatcgttaaa
     1441 gcattctaga cctttaacag gatactcatc gcgaaattca aaaagatgtt tgaacatacg
     1501 ggttgccgct cccgaggctg gaacaaactt caccttgctg ccctggaaat ccttgaacat
     1561 ctcaaccaaa tcgtccacct catcatcttc aaggctaata ataccatcgc caacggttgc
     1621 aggcctctcg ataggcaaaa agggaaatcc ctctcgaaac atcctcatgt acccttctac
     1681 ctgttgagga gtaatccccc tttcctccag ctgcttcaaa tccaacttgg tgagcataat
     1741 ggttcggttt accgttaata aacagcgtca atttactaac aattcacatt cactccaaat
     1801 ttctccagtc aaattgaatt ctacagtaat ttttttcacc ttccagccta ctgttgatat
     1861 ttatcaaggt ggaattgcct ggtatatttt ttcgatttat caagatggaa tcgccctcct
     1921 ttgcctctga attaaagtta accgaaattt tctttggaaa atattttctt agagaactga
     1981 tggtgaatgt gttatatgcc caatcaatgt agcgaacatt gttaacatgc aggttcatgt
     2041 ctatatcacc tgctctgact tcaaactgct cggtaaaggc aggctgaacg atagattcgg
     2101 gaattttggc agcattacga tttaaagccg agcgggaggc aaaatcagat tcaagatacc
     2161 attcggcaat tctcacaggg cgacggtttt caacaccaac caccaaccag ctgctagtag
     2221 cagcgattag cttattacca ttcgaatcat agaactcaaa atcacgaagt gcaaaaattc
     2281 catcggcacc acgaggccag gtaaccagcg taaccgcctc ctgccatacg g
//
LOCUS       DAOOQX010000296         2322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_56520_length_2322_cov_3.532863, whole genome shotgun sequence.
ACCESSION   DAOOQX010000296 DAOOQX010000000
VERSION     DAOOQX010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56520_length_2322_cov_3.532863"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="PLX53_05530"
     CDS             <1..1435
                     /locus_tag="PLX53_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOY72578.1"
                     /translation="LSFAGNDLVNFIGPTMAALKTYQVHLANPGVAPDQLLMTDLAGP
                     VSTNTLYLLIAGLVMVITLWTSRKARTVTNTEVGLANQDSGAERFGSTQLSRSIVRIS
                     RSFSEGVGRITPLRIKSFLSRRFDLSKALVVKNPKDAPAFDLVRASVNLVVSSILISL
                     GTSLKLPLSTTYVTFMVAMGTSLSDRAWGRESAVYRVTGVITVIAGWFFTALITFIVS
                     FVVAAALFYWKAFALVGLAILAGWLLIRSNLIHRSRNQNRDVGAELITDKSVVEKCTL
                     GVNNSLRDVVEVFSQTIDGLTSENRKLLRTVNNRVADLNSNSKNFKNNIYQTLKKLEA
                     DSVETGHYYVQVVDYLREIAHSLSFITEPSLKHIENQHKGLTTDQAIELTTISNQVSD
                     FFQEIMKIIKNNDFTGVPQVVSKQQDILDKLNEARKNQVKRIKQGVTGTRNSVLYLGL
                     INEIKNIMLHTVNLLKAQRDFILNNVE"
     gene            1516..>2322
                     /locus_tag="PLX53_05535"
     CDS             1516..>2322
                     /locus_tag="PLX53_05535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOY72579.1"
                     /translation="MELWTIEHTFIRMKAIKVSFCTTSNLITDQRIQRAASTLSRNGY
                     QVNVFALRRHGLPNEKPFEFAVKRFRCVFSDGPMFYVEYNLKILTRLIFTRTSLIYSN
                     DLDTLPGCWLASILKAKPLVYDSHELFTEIPELIGHPVKKRFWAIAERVCIRRAKCVI
                     TVSEGVAKELSTRYGVKPVVVRNFSVKRDKSPYRDLKPTLIYQGSLNVGRGIELAIDM
                     MEWLPCYHLLVIGSGDIEIPLRRRAIERGLTDRVEFMGRVDPDRLPDVTGR"
BASE COUNT          614 a          514 c          554 g          640 t
ORIGIN      
        1 cctttcgttt gccggtaacg acctggtaaa ctttattggc cccaccatgg cggcgctcaa
       61 aacctaccag gtacacctgg caaaccccgg agtggcaccg gaccagctgc tgatgaccga
      121 tttggccggt ccggtctcaa caaatacgct ttacctacta attgcaggat tggttatggt
      181 gattacccta tggacatccc gtaaggcacg aaccgttacc aataccgaag ttggactggc
      241 caaccaggat agcggggctg aacgttttgg cagcacccag ctctccaggt ctattgtgcg
      301 tatatcccgt tctttttctg aaggtgttgg gagaatcact ccactacgca ttaaatcatt
      361 tctttcccgg agattcgatt taagtaaggc tttagtggta aaaaacccca aggatgctcc
      421 tgctttcgat ttggtacgtg cttctgttaa cctggtggtt tccagcatac tcatcagctt
      481 gggaacttcc ctaaaactac cgctttctac cacctatgtt acctttatgg ttgccatggg
      541 aacctcgctc agcgataggg cgtggggaag ggaaagcgct gtttaccggg ttaccggtgt
      601 tattaccgtg attgcaggtt ggttctttac agctctcata acctttatcg tatcgtttgt
      661 tgttgctgct gctcttttct actggaaagc ttttgccctg gtagggttag ccattcttgc
      721 cggttggctg ctcatcaggt cgaacttaat tcaccgcagt agaaatcaga atagggatgt
      781 tggtgcagag ctaattaccg ataagagtgt tgttgaaaaa tgcacattgg gggttaacaa
      841 ctccctaaga gatgtggttg aggtgttttc ccaaaccatt gacgggttaa cctccgaaaa
      901 caggaagctg cttagaacgg tcaacaaccg cgtagctgac cttaattcta attccaaaaa
      961 ttttaaaaac aacatctatc agacactaaa gaagctggaa gccgattctg tcgaaacagg
     1021 ccactactac gttcaggtgg ttgattacct cagggagatt gcccattcac tctcgtttat
     1081 aaccgagccc agccttaagc acattgagaa ccagcacaag gggctaacta ccgatcaggc
     1141 cattgagtta acaaccatca gcaaccaggt ttcggatttc tttcaggaga taatgaaaat
     1201 aataaagaat aacgacttta ccggagttcc acaggttgta agcaagcagc aggacattct
     1261 tgacaagctt aacgaggcaa gaaaaaatca ggttaagcga attaagcagg gtgtaaccgg
     1321 tacccgaaac agcgttctgt acctcgggct catcaacgaa attaagaata tcatgctgca
     1381 caccgttaac ctgctcaagg ctcaacgcga tttcattctg aataatgttg agtagtggtc
     1441 ttccagcatg gtcccattta gctttcttgc tgaaaaacat tatggggttt gccagcttgc
     1501 taaaaggtgt atgatttgga attatggact atagagcata catttatccg gatgaaagcc
     1561 attaaagtaa gtttttgtac tacctcaaat ctgataaccg accagcggat tcaacgagca
     1621 gctagcacgc tttccaggaa tgggtatcag gttaacgttt ttgccctcag gaggcatgga
     1681 ctaccgaatg aaaaaccttt tgaatttgca gtaaagcgtt ttcgttgtgt gttttcggat
     1741 ggtcccatgt tttatgtcga gtacaatctt aaaatcttaa cccgccttat ttttacccgc
     1801 actagcttaa tatactccaa cgatttggat acacttccag ggtgctggct ggcttcaatt
     1861 ttaaaagcca aacctttggt ttacgatagc catgagcttt ttaccgagat accagagcta
     1921 atagggcatc ctgttaagaa acgtttctgg gccattgccg agcgggtatg tattcgaagg
     1981 gcaaaatgcg tcattacagt ttcagagggt gttgccaagg agctgagcac caggtatgga
     2041 gttaagcccg ttgttgttcg taacttttcg gttaagcgtg ataagtctcc ttatagggat
     2101 ttgaagccaa cgttgatata ccaaggatcg ttgaatgtgg ggagaggcat agagctcgcc
     2161 atcgacatga tggaatggct cccctgctac catttactgg ttattggaag tggcgatata
     2221 gaaatacctc ttaggcggag agctattgag cgggggctaa ccgacagggt tgagtttatg
     2281 ggcagagttg atccggacag gctacccgat gtaaccggta gg
//
LOCUS       DAOOQX010000297         2322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_56536_length_2322_cov_2.291575, whole genome shotgun sequence.
ACCESSION   DAOOQX010000297 DAOOQX010000000
VERSION     DAOOQX010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56536_length_2322_cov_2.291575"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..749
                     /locus_tag="PLX53_05540"
     CDS             135..749
                     /locus_tag="PLX53_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOY72580.1"
                     /translation="MGLAASIPLGPVGVVCVQRTINKGRISGFFSGLGAATTDTIFAA
                     IGGFSLTFIIGFIEAKQQIIELAGGIIIILLGLRTFYTNPVTQLKRHKKKPNTLVEDF
                     ISMFLLTGANPFTIFLFIALFAALGIVTKNQNLLFTATALVGVFLGASLWWFTLSSLV
                     NLFRHKFRLKQLWWMNKITGSIIFILGIIAIVGVVNKAWFGQLP"
     gene            1126..>2322
                     /locus_tag="PLX53_05545"
     CDS             1126..>2322
                     /locus_tag="PLX53_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOY72581.1"
                     /translation="MKIAEKILTFFTLKIERKTARDSADRLVQLSIVTIPFLLYLVVF
                     CTLLLFRHQVASGIIAGVAAISILLIIVLAYSKKRVNSFGLVALAISGIALLAILIVG
                     KLDAALMIGVAMFPMLALSTTTPNRAHIISFIFWILYSCILITGPKFIGESVSSTGYY
                     RLLSIIFYPLLQLVASLLYLFKAKRLQELEKQLIEQKNQNKLREDYLAKLSHQLRTPL
                     NNIMVISNMLSNTITEDKYRDLIDTIQASTNSLAGVVNGMVEKSSTGIEPETDFAIPF
                     NLSDTINNTIRLFATQSQTVNFNIKIDESIPKTLKGDPVKLKQIFLNIIENVIKNKEG
                     SKVNIEIIASRMGNAKNSLELFFEVKSNVPLLIHQNEGYSQTVIADSTSSSLNNQFFI
                     DLLDLTI"
BASE COUNT          641 a          515 c          461 g          705 t
ORIGIN      
        1 gtcataattt tggttaatca gctttagcct caagcctaac ctagactata tttattatat
       61 ttgccaactt gtatgtagtt gtaatattga agggacatct aatgattttt agcctgcttc
      121 taaaaggaat cataatgggg cttgccgctt caattccgct cggacctgtt ggtgtggtat
      181 gcgttcagcg tacaattaac aaaggaagga taagcggatt tttctcgggt ttaggtgctg
      241 caaccacgga tactatattt gcagcaattg gcgggttcag cttgaccttt attattggat
      301 ttattgaagc caagcagcaa atcattgaac ttgcgggggg gattattatt atcctactcg
      361 ggttaagaac attctacact aatccggtta cccagcttaa gcgacacaaa aagaagccca
      421 acacccttgt tgaggatttt atctccatgt tcttgcttac cggagccaac ccatttacca
      481 tttttctttt tattgccctg tttgctgccc tgggaatagt tacaaagaat caaaaccttc
      541 tttttacggc taccgcactg gttggcgttt ttttaggggc aagcttgtgg tggtttaccc
      601 ttagcagcct ggtaaacctt ttccgccata agttccggct caagcagctc tggtggatga
      661 acaagataac cggttcaatt attttcattc ttggcataat tgctatcgtt ggagtggtta
      721 ataaggcatg gtttggacaa cttccctgat gctgcctttc cctggccatc caaatctttc
      781 atccgttaat acaatctttt attaccagat gcacggggat tttgtgaagc caatcaggca
      841 atcaccaata gtgcgttttg ttttcaacgg catggattgt aaagcgcaaa ccatcgggtt
      901 tgtatattat aggcgttacc tacctttacc tgagtattcc aggtcattaa agattaggcc
      961 tcattatgtt gaataagcag ctttacattt ttctattagg gttgttctgc ggcagcaccg
     1021 cgagggaagc ataagataca ggtaaaattc tgctcgtgcc gattattgct tttacatttt
     1081 ttaaactacc tttggtagca atctttactc aattttctta aagcaatgaa aatagctgaa
     1141 aaaatcttaa cctttttcac gttaaaaatt gaaaggaaaa ctgcaaggga ttcagccgat
     1201 agactggttc aactaagcat tgtaaccata cccttcctac tctatcttgt tgtcttttgc
     1261 acattgctgc tttttagaca tcaagtggca tcggggataa ttgcaggcgt tgctgcaata
     1321 agcatattac tcatcatcgt tttggcttat agcaagaagc gggttaactc attcggatta
     1381 gttgcgttag ctatttcagg tatagcccta ctggccattt tgattgtcgg taaactcgat
     1441 gctgcattaa tgattggggt agccatgttc cccatgctgg ccttatcaac aactaccccc
     1501 aacagagcac atattatttc ctttattttc tggattctat actcgtgcat tctcattacc
     1561 ggccctaaat ttattggtga aagtgtcagt tcaacgggct actaccgttt actttccatc
     1621 atattttacc cgctgctgca gctagttgcc tcgctgcttt accttttcaa ggcaaagagg
     1681 ctacaggaac tcgaaaagca gctgattgag cagaagaacc agaataagct gcgcgaggat
     1741 tatttggcca agctttccca ccagcttcgc acacccctga acaacatcat ggtcatatcc
     1801 aacatgctgt caaacaccat caccgaggac aagtacaggg atctgattga caccattcag
     1861 gcatccacta acagcttggc aggtgtagta aacggcatgg tggagaaatc gagcacggga
     1921 atagagcctg aaacagattt tgccattccc tttaacctat ctgacactat caacaacact
     1981 attcgcctat ttgccacgca atcgcagacg gttaacttca acattaaaat tgatgaaagt
     2041 attcccaaaa cactaaaggg cgaccccgtt aagctgaaac aaatttttct caacattatt
     2101 gagaatgtaa tcaagaataa ggagggaagc aaggttaaca tcgagattat tgccagccgg
     2161 atgggcaatg ccaaaaactc cctcgagcta ttttttgagg taaaaagcaa cgtgccccta
     2221 ctcatccacc aaaatgaggg gtattcgcaa actgtaattg ccgattcaac ctcctcttca
     2281 ttaaacaacc aatttttcat tgacctgcta gacctgacca ta
//
LOCUS       DAOOQX010000298         2321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_56565_length_2321_cov_3.041924, whole genome shotgun sequence.
ACCESSION   DAOOQX010000298 DAOOQX010000000
VERSION     DAOOQX010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56565_length_2321_cov_3.041924"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..772
                     /locus_tag="PLX53_05550"
     CDS             194..772
                     /locus_tag="PLX53_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72582.1"
                     /translation="MKMKSMIMVAAAFTFAAVLSSCGGSKKAADSSFGKEVSVPCNDD
                     EFHSDAKFFRGTGNGISQDLSTAKSKARLDANTNLARSINTTIKSVSDRYVNERQIGE
                     NSEFEQKFEQMSREVVNQQLNNVEVACNKTMQTKDGKFQVFTALQVSKDEVLNNISNR
                     ISKDQKLQLDYDKMKFEQIFNEEMNKLESERP"
     gene            883..2175
                     /locus_tag="PLX53_05555"
     CDS             883..2175
                     /locus_tag="PLX53_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72583.1"
                     /translation="MRKILLLTAAMVCLAGYTFSQDKVLDKSGKKPKWVNGIEKDYII
                     VAGSGPDLQVAQQNALTSIKESIVTSVAQNVKSTSTIQKEEANYNNNVNIFLEKFAST
                     TTAQSGKVPFLQGISLSKVEDFYWEKLQRPDKSIIYTYHIKYPFPEFELMKLVSDFKM
                     RDNELTRQLEDLLAQVDQVESVEQIEKNMAELASLKEYFMDARVDQCNLGITRYRSLL
                     NSIELVELESSLGELKYYLRLGGRSISTTKKPVVKSECARVTSIVNNVDNVTIKYDYD
                     NCYEDPENNILVKYRFGVADVQKPFYFDVAANKASIFVSDPFNFTAISKDSTNVVSAL
                     LDMTVRSKYDSPFTIDKVVIEWKGIPPVVIDNIGQSFQGKGNHNLKLNVEQPIKIAGT
                     SSAGKTLSLISGYIYYRSNNTGESKTYRIYNHSYTTDW"
BASE COUNT          682 a          489 c          546 g          604 t
ORIGIN      
        1 ttccattgag aaggacaaga tgatgataca ggcaggcaag gaggtagccc agtcttgggc
       61 ttactggggc gctgcctccc acttcaactg ggattggagc tggctttacg ttaaaaagta
      121 attcacaact agcaaaaaat ttttctaccc attattttaa cttaatccga agtttaactt
      181 taaattgaaa gctatgaaaa tgaaaagtat gattatggtt gctgctgctt ttacctttgc
      241 agctgtttta tctagctgcg gtggttccaa aaaggctgca gattcttcat tcgggaagga
      301 agtttcagtt ccctgcaatg atgacgagtt tcattccgat gccaagtttt tccgtggaac
      361 aggtaatggc atcagccagg acctaagcac tgccaagagt aaggcaaggc tcgatgccaa
      421 tactaacctg gctcgcagta taaacacgac cattaagtcg gtatctgaca ggtatgttaa
      481 cgagcgccaa attggcgaga attcagaatt tgagcagaag ttcgagcaaa tgtcgcgtga
      541 ggttgtaaac cagcagctca acaacgttga ggtagcttgc aataaaacca tgcaaaccaa
      601 ggatggaaag ttccaggttt tcaccgcgct ccaggtttcc aaggatgagg tgctgaacaa
      661 catttccaac cggatttcga aggatcagaa gctgcagctc gactacgaca agatgaaatt
      721 cgagcagatc ttcaacgagg agatgaacaa gctggaaagc gaacgccctt aatttgataa
      781 cccaagaatt cagcaacccc catcggggtt gctgattact tatggtaagt gcttgaattg
      841 ttataatctc ccattcttta cgatggggaa atttaattag ctatgagaaa aattcttttg
      901 ctgaccgccg cgatggtttg cctggcagga tatacctttt cgcaggataa ggtgttggat
      961 aaaagcggaa agaaacccaa gtgggttaat ggtatagaga aggattacat aattgttgcc
     1021 ggcagtggcc ccgatttgca ggttgcgcag cagaatgctt taacctccat taaggagagt
     1081 atcgttacgt cggttgcaca aaatgttaaa tctacctcca ccattcagaa ggaagaggct
     1141 aactacaaca ataatgtgaa tatattcctc gaaaagtttg cctccaccac aacggcccag
     1201 tcgggtaagg tgcctttcct gcagggcatt tcgctatcaa aggttgaaga cttctactgg
     1261 gagaagctcc agcgtcccga caaaagcata atttatacct atcacattaa gtaccctttt
     1321 cctgagtttg agctgatgaa gctggtaagc gatttcaaaa tgcgcgataa cgagttgacc
     1381 cgacagctgg aagatttgct agcccaggtt gaccaggttg aatcggttga gcaaattgaa
     1441 aagaacatgg ccgaacttgc aagtctaaag gagtacttca tggatgccag ggttgaccag
     1501 tgcaacctag ggatcaccag gtaccgctcg ctgcttaact ccattgagtt ggttgagctg
     1561 gagagcagcc tgggagagct taagtactac ctccgactcg gagggcgttc gatttcaaca
     1621 acaaaaaaac ctgttgttaa atcggaatgc gcaagggtta cctcaattgt taacaatgtt
     1681 gataacgtaa ccatcaagta cgactacgat aactgctacg aggatccgga aaacaacatt
     1741 ctggtaaagt acagatttgg agtggccgac gttcaaaaac ccttctactt tgatgttgct
     1801 gccaacaagg cttcaatatt tgtgagcgat cccttcaact ttactgccat atcaaaagat
     1861 agcactaatg tggttagcgc tctgctggac atgacggttc gttcgaagta cgattctccc
     1921 tttaccattg ataaggtcgt gattgagtgg aaagggattc ctcctgttgt tattgataac
     1981 attggccaga gttttcaggg aaagggcaac cacaacctca agttgaatgt tgaacagcca
     2041 attaagattg ccgggacatc ctcggcaggt aaaactcttt cgttaatttc gggctacatc
     2101 tactacaggt caaacaacac cggtgagtct aaaacttaca ggatatacaa ccatagctac
     2161 actactgatt ggtagaagac ttgaaggcta aaaaaagaaa ggcctgaaag gccttttttt
     2221 attccttgta ggactcaagc cggaatggga gttttatgtg gtcggagagg tcttttccgg
     2281 catccttgat gtcaatatca tcctcgttgt aggcccaaac c
//
LOCUS       DAOOQX010000299         2316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_56728_length_2316_cov_3.146838, whole genome shotgun sequence.
ACCESSION   DAOOQX010000299 DAOOQX010000000
VERSION     DAOOQX010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56728_length_2316_cov_3.146838"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="PLX53_05560"
     CDS             <1..1214
                     /locus_tag="PLX53_05560"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOY72584.1"
                     /translation="IHDASCFILNSNGRPIIPLTLGEDFVCRGFTGSGHVKGEAVFAG
                     FGMKQPNYDDYRNINVKGKIVVAFKSAPPWASETGSWGDTSPRAKARTAKEQGAAGII
                     FIADPKVFPPTMIYGSIACGDGPHLFDFPMIQLSDRATDSLFAELPVKAPNLYEQICQ
                     DKAPHSIELGRSVLVNVKAEYFAEKPTANVVGIVEGSDSLLKNEYVVLGAHLDHVGYQ
                     GEKLYFPGANDNASGVAALIAIAEALKKSKVEIKRSIIFIAFSSEESGLRGSKYFVDH
                     SPVDLSEVVAMLNFDCVGQGDSIAIGGRLSFPKLWKKVKKIDKHTTKLLSTRTFGGGG
                     ADAEAFYRAGISTLYFNTTNGYKHLHSPSDRAETINPELIEKVAKLGYLVTVDLANGR
                     YKGERDRQRKK"
     gene            complement(1288..1827)
                     /locus_tag="PLX53_05565"
     CDS             complement(1288..1827)
                     /locus_tag="PLX53_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOY72585.1"
                     /translation="MRILVFCVLMTVVGTTAGQTGSFYDFTVKDIDGNDFPMSSLKGK
                     KIMVVNTASKCGYTPQYADLQALYEKYQDQNFVVIGFPANNFMKQEPGSNKEIKEFCT
                     STYKVSFPMMGKISVKGKDMHPLYGWLTSKSLNGVMDSEVKWNFQKYLIDERGILVMV
                     IDPGEKPNSERVIKWIEGK"
     gene            complement(1893..>2316)
                     /locus_tag="PLX53_05570"
     CDS             complement(1893..>2316)
                     /locus_tag="PLX53_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOY72586.1"
                     /translation="EFMLVTAAKNFRSWFSSNTWAYIAFFAFLFVMVAVAFYIFTNRY
                     TIKRLTFAGSILLTIVFVISITCSISLRNQSIQRNHAILFAPVVSVKSSPASTGSDIF
                     ILHAGTKVMVTRSLGEWSEIKIADGSKGWVLSSTFERI"
BASE COUNT          711 a          483 c          490 g          632 t
ORIGIN      
        1 tcatacatga cgctagctgc tttatcctaa atagtaatgg acgccccatt atcccgctta
       61 ctcttggtga agactttgtg tgccggggtt ttacaggatc gggtcatgtg aagggcgaag
      121 ctgtttttgc cggatttggc atgaagcaac ccaactacga tgattaccgc aacataaacg
      181 tcaaaggcaa gatagtggta gcattcaaat cggctcctcc ttgggcgtca gaaacgggca
      241 gctggggcga cacctccccg agagcaaaag cgagaactgc taaagaacaa ggagcagcgg
      301 gaatcatttt tatagccgat ccaaaggtct ttccgcctac catgatatat ggaagcatag
      361 catgtggcga tggcccccac ctgtttgatt ttccaatgat tcaacttagc gatagagcaa
      421 cggacagctt gtttgccgag ctgccggtaa aagcccctaa cttgtatgag caaatttgtc
      481 aagataaagc tccgcactca attgagctgg gtagaagcgt tttagtaaat gttaaagctg
      541 aatactttgc tgaaaaaccc actgccaatg tagttggaat tgttgaggga agcgattcgc
      601 tgcttaaaaa cgagtacgtt gtgctgggag cacatctcga tcatgtgggg taccaaggtg
      661 aaaagctcta ctttccgggt gcaaatgata acgcttcagg agtagcagcc ttaattgcaa
      721 ttgccgaggc tttaaaaaaa tcgaaggtgg aaattaaacg ctccataata tttattgcct
      781 tttcatctga agaatcaggg ttgcgcggtt ctaaatattt tgttgaccat tccccagttg
      841 acctttcaga ggtggttgcc atgcttaact tcgactgtgt tggacagggc gatagcattg
      901 ccattggagg aaggttgagt tttcctaaac tgtggaaaaa agttaaaaaa atagacaagc
      961 acacaacaaa actactttca accagaacgt ttggaggggg tggagccgat gcagaggcat
     1021 tttacagggc gggtatctct acgctgtact tcaatactac taatgggtac aagcatctcc
     1081 attctccaag cgacagggct gaaacaataa atcctgaact gatagaaaaa gtagcaaaac
     1141 taggatattt agttacagtt gatttggcaa atggtaggta taaaggtgaa agagacaggc
     1201 aacgaaagaa gtaaatccct atttatgaat ctgtaaaaaa ttcatcatcc ttctgtgtaa
     1261 atcaactggc agaccttcct gttataatta ttttccttca atccatttga ttaccctttc
     1321 agaattgggt ttttctccag gatctataac cattaccaat atgcccctct cgtcaataag
     1381 gtatttctga aaattccact tcacctcgct atccatcact ccattcaagc ttttacttgt
     1441 aagccagcca tataagggat gcatatcctt ccccttaaca gatattttgc ccatcatcgg
     1501 aaaggaaact ttgtaggttg aggtgcaaaa ctcctttatc tccttatttg aaccaggctc
     1561 ctgcttcata aaattattcg ctggaaaacc aatcactacg aagttttgat cctgatactt
     1621 ttcgtataac gcttgcagat cggcatactg tggggtatag ccacacttag aagcagtatt
     1681 gactaccatg atttttttgc cctttaggct tgacatggga aaatcgttac catcaatatc
     1741 ctttacggta aaatcgtaaa atgatcctgt ttgtccagct gtagtgccaa caacagtcat
     1801 gagcacgcaa aaaactaata ttctcatcgg attggaagtt ttagtactat ctgcatacta
     1861 aaacagctgc gtgcagaaaa tgttcgaata agctatatcc tttcaaaggt tgagctaagc
     1921 acccagccct tgcttccatc ggcaattttg atttcgctcc attcaccaag cgaacgggta
     1981 accattacct ttgttcctgc atgaaggata aaaatatcac taccggttga ggctggagaa
     2041 cttttaactg aaacaacagg ggcaaataga atagcatgat ttctttgaat ggactgatta
     2101 cgaaggctaa tactgcaggt aatggaaata acaaaaacaa tagtaagcag tatagatccg
     2161 gcaaaagtta agcgtttgat ggtgtacctg ttggtaaata tataaaacgc aaccgcaacc
     2221 atcacgaaaa ggaatgcaaa aaaggcaata taagcccatg tattagagga aaaccaactt
     2281 ctgaaatttt tagcggctgt caccagcata aactca
//
LOCUS       DAOOQX010000300         2313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_56841_length_2313_cov_4.408769, whole genome shotgun sequence.
ACCESSION   DAOOQX010000300 DAOOQX010000000
VERSION     DAOOQX010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56841_length_2313_cov_4.408769"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..885
                     /locus_tag="PLX53_05575"
     CDS             283..885
                     /locus_tag="PLX53_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOY72587.1"
                     /translation="MKSKTWITIGIVVLVAVILYISIKGSYNKMVQKDEAVTSQWANV
                     ENVYQRRLDLIPNLVNTVKGYANFEQQTLTQVVEARAHATQVKVSPENLNQESIQQFQ
                     AAQNELSGALSRLMVVVEQYPELKANQSFLDLQAQLEGTENRIAVERNKFNEVVRDYN
                     TYIRRFPSNIWAALFNFDKRGYFESEAGAEKAPKVDFGTN"
     gene            952..1386
                     /locus_tag="PLX53_05580"
     CDS             952..1386
                     /locus_tag="PLX53_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOY72588.1"
                     /translation="MKAKDYFTLEEQKRITDAVEQAEKNTSGEIRVHVENECRIDVLD
                     RAVQIFAMLKMHKTKLRNGVLIYLSLKDHKFAILGDAGINAKVPEDFWDSTKQAMLEE
                     FQENRLTEGIIKGITMAGEKLKEHFPYKQDDVDELTNDISFG"
     gene            1416..2201
                     /locus_tag="PLX53_05585"
     CDS             1416..2201
                     /locus_tag="PLX53_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOY72589.1"
                     /translation="MITKQLRNILLAAALLLPNISWSQDIPDPMNPPRLVNDFAGILG
                     GEERDALESMLRGYHDSTSTQIYVVTVNDLSGYDISDYAFRLGEKWGVGQKSKNNGVV
                     ILIKPKVGNQKGQAFIATGYGMEAIIPDAVARRIVDNEMIPYFKTNSYYKGIVAGCKV
                     IIDLAGGEYTADAYYKKESPIPGIILLIIFFGFIFLSGGRRSKLRSGSIGKNIPFWIL
                     LGSMGSGRRGGGFGGFSSGSGGFGGFSGGGGGSFGGGGAGGSW"
BASE COUNT          676 a          503 c          572 g          562 t
ORIGIN      
        1 gaattttctt tcgtttgggc gcatggttaa cgtccgggtc ccatggctga agggcgggta
       61 actcgtcggg gatgccctga agtatctcct tttgaaattc ttcgcgtgtc atactttttg
      121 aatgttttta acctagtgtt ttgccacaaa tttaagattt taaaccgact ttgaacggct
      181 tacccgaaat tgtaaattat attaatttaa ccggtatgca ttttttcatt tgataaataa
      241 agctactttt ggcaagttta aaacatatca aattaaagaa ctatgaagag taaaacatgg
      301 ataaccattg gaatagtagt gttagtggct gttatactat atatttccat taaaggatcg
      361 tacaacaaaa tggttcaaaa ggacgaggca gttacctcgc aatgggccaa cgttgaaaat
      421 gtataccagc gcaggttaga ccttataccc aacctggtga atacggtgaa gggctatgcc
      481 aactttgagc agcaaaccct gacccaggta gtagaggcca gagcacatgc cacacaggta
      541 aaggtttcac cggagaatct gaatcaggag agtatccagc aattccaagc cgctcagaac
      601 gagctgagtg gggcgctttc gcgcttaatg gtggtagtag agcagtaccc cgagcttaag
      661 gccaaccaga gttttctcga ccttcaggca cagctggagg gaacagagaa tcgtatagct
      721 gttgagcgca ataagttcaa cgaagtggtt agggactata acacctacat aaggcgattc
      781 cccagtaata tttgggcagc actattcaac ttcgataaga ggggatactt tgaatccgag
      841 gccggagccg aaaaggcgcc caaagttgat tttggcacaa actagccaaa tatatagaga
      901 tagctggcca caactttacg ctggcacaac catgcccatc aatccaaaaa aatgaaggca
      961 aaggattact ttactctcga ggagcaaaaa agaattacag acgcggttga gcaagccgaa
     1021 aaaaacactt caggcgaaat cagggtgcac gtggagaacg aatgccgaat tgatgtgctc
     1081 gacagggccg ttcaaatttt tgccatgcta aaaatgcaca aaacaaagct gagaaacggc
     1141 gtgctgatct acctctccct aaaggaccac aagtttgcca tactgggcga tgccggaata
     1201 aatgcaaagg ttcctgaaga tttttgggat tcaaccaagc aggcgatgct tgaagagttt
     1261 caggaaaaca ggctgactga agggataatt aagggaataa ccatggctgg cgaaaagctc
     1321 aaggagcatt tcccctacaa gcaagacgat gtagatgaac tcaccaacga tatttcgttt
     1381 ggctaaaccc attcttcaca ttaagcaacc caaccatgat caccaaacaa ctcaggaata
     1441 ttcttctagc ggcggcgcta ttgcttccaa atatttcgtg gagccaggat attcccgacc
     1501 ccatgaatcc accccggctg gttaatgatt ttgccgggat tctcggaggc gaagagcggg
     1561 atgcactcga atcaatgctg cgtggctacc acgactccac ctcaacccag atatatgttg
     1621 taacagtaaa cgatctatct ggctacgaca tatccgacta tgctttccgc ctgggcgaaa
     1681 aatggggagt tggccaaaaa agcaaaaata atggcgtcgt tatcctgatt aagcctaagg
     1741 tagggaatca gaaagggcaa gctttcatag ccaccggtta cggaatggag gctataattc
     1801 ccgacgccgt tgctcgcaga attgtggaca acgagatgat tccctacttc aaaacgaaca
     1861 gctactacaa ggggattgtg gcgggctgca aggtaataat cgacctggcc ggaggagagt
     1921 acactgctga tgcttactac aaaaaggaat cgcctattcc gggaattatc ctcctgatca
     1981 tattttttgg attcatattc ctttcgggcg gcaggcggtc aaagctacgt tcaggatcta
     2041 ttgggaaaaa cattccgttc tggatcctac taggctcaat gggcagcgga agacgtggtg
     2101 gtggatttgg gggattttct tccggctcgg gcggatttgg aggatttagc ggaggaggcg
     2161 ggggtagctt cggcggtggg ggtgccggag gaagctggta acctgccgat ccttattctt
     2221 tgcagctaat catctcaact gtacattcta ctctgtactc tccgtggtta tgtctttctc
     2281 accacagaga gcacggagca gcacagagag agt
//
LOCUS       DAOOQX010000301         2311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_56925_length_2311_cov_3.503989, whole genome shotgun sequence.
ACCESSION   DAOOQX010000301 DAOOQX010000000
VERSION     DAOOQX010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56925_length_2311_cov_3.503989"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(327..>2311)
                     /locus_tag="PLX53_05590"
     CDS             complement(327..>2311)
                     /locus_tag="PLX53_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOY72590.1"
                     /translation="AKIVSNILNKLGYDDENLGINTAIVLPEEQLLLPVISALPTNLD
                     EVNITMGYPINDTFAYSLADGLISLQANLKTAGTEVKFYNKDVLNLLSHPYVVQLAKE
                     ESKSISATIVQQKSISVDGSYFKDSPLLSKIFSQASYLPTYLLDVIKTVSGGLASLEN
                     DRPESIKLELEFLFAIYKALNGLDGIISQFDTTPSPRTFRSIFRRAMAEQRVSFYGEP
                     LSGIQLMGFLETRSLDFENVIILSLNDGVMPGPSNSPSFITPSIRKAYGLPDYRHQNA
                     IYAYYFYRLLQRASNIYMVYTNSSESMRTGEVSRYVLQLLMESNLKINRIQVKFDLGI
                     TPDGPISVSKTDRVMAILERYLANGQSSKYLSPTAMVAYKNCKLRFFFSHVAGFKEPE
                     EMDEAIDERGVGIILHRAIELIYDGIKSSVTSTSVGAILKNDNLIEANIRKAYAENFN
                     VDLSKFDEVVIGRNALLLERIKWMVRQMLVVDMKRTPYSITRSEQSEMVDIPIVVDGR
                     NFAVKLGGRIDRVEQANGLTRIIDFKTGKFEPNKVTFSEIDQLFENKSNTDGILQLFA
                     YCMIYSMKYGIDAGSIQPNLWYVRKNTLPLISKSLKKGSSAKTEVTTFESLQAPFRQM
                     LVALLEEIFNPSVDFTQTDNTDNCKICPYNAICNRV"
BASE COUNT          599 a          512 c          515 g          685 t
ORIGIN      
        1 ccggattata ctgtcaggac ctgggacttt ggtggattcg atgcatggcg acagcaactt
       61 ttgaactccg gacaacttgg ctgctggtgg cagcagtagc atattgtttg tgtttgggtt
      121 acttctatag tagaaaaatc gtgtcaccaa cgccggaacc caagctgttt tttataacat
      181 actgcatatc tgcaatataa tcttatcctt ccgcatttct agctgttgcc tgctgccatt
      241 ttgaggaata aaatccccaa attgtagagt tttgaggagt ggttgacctg ttgctttcca
      301 cactcatgcg ttttttcttg acaaaattat accctgttgc agatggcgtt atacgggcat
      361 attttgcagt tatcggtgtt gtccgtttgg gtaaaatcaa cggaggggtt gaaaatttct
      421 tccagcaaag ccaccagcat ttgtctaaat ggtgcttgta aggattcgaa ggtggtcact
      481 tcagtcttag cgctgcttcc cttttttaaa gatttggata taaggggaag agtatttttt
      541 cgcacatacc aaaggttggg ttgaatgctg ccggcatcaa tgccgtactt catgctgtaa
      601 atcatacagt aagcaaatag ctggagtatg ccgtcggtgt tgctcttgtt ttcaaaaagc
      661 tggtctattt ccgaaaaggt taccttgtta ggttcaaact taccggtttt aaagtcaatt
      721 attcgggtta gaccatttgc ctgttcaacc ctatcaattc gtccacctag cttcactgca
      781 aaatttcttc catccacaac aataggtata tcaaccattt cgctctgctc agaccgggta
      841 atactgtatg gagttcgctt catgtcaaca accagcattt gtcgaaccat ccattttatc
      901 ctttccaata gcagggcgtt gcggcctatg acaacctcat caaactttga aaggtcaacg
      961 ttgaaatttt cagcgtaagc cttcctgata tttgcttcaa ttaaattgtc gttcttcaaa
     1021 atggcgccaa cgctcgtact ggtaacgctt gatttgatac catcatagat gagctcaata
     1081 gctctatgaa gaattatccc cacccctcgc tcgtctatgg cctcatccat ttcctccggt
     1141 tccttaaagc cggcaacatg gctgaaaaag aaacgcagct tacagttttt gtaggcaacc
     1201 atcgctgttg gcgagaggta ctttgaactt tgtccattgg ctaaatacct ttccagaatg
     1261 gccataaccc tatctgtttt gctaaccgat attggaccat caggggtaat tcccaagtcg
     1321 aattttacct gtattcggtt gattttcaag ttcgactcca taaggagctg cagcacgtaa
     1381 cggcttacct cgcccgtgcg catgctttcg gaactgttgg tgtaaaccat atagatgttg
     1441 ctggcccgct gaagcaagcg atagaagtag taggcataaa ttgcattctg atgacggtag
     1501 tcgggcaacc cgtaagcttt ccttatgctc ggagtgatga atgatggaga gttggaaggt
     1561 ccgggcatca ccccatcgtt cagcgaaagg ataatcacat tttcaaaatc gagggagcgg
     1621 gtttccaaaa agcccatcag ctggatgccg gacaacggtt caccgtagaa tgagacccgt
     1681 tgctcggcca tcgccctgcg aaatattgac ctgaaagtcc ttggtgaagg agttgtgtcg
     1741 aattgggaaa taattccatc caacccgttc agcgccttat aaatggcaaa cagaaattcc
     1801 agctccagct ttatgctctc cggcctatcg ttttccaacg atgctagtcc accggaaaca
     1861 gtcttaataa catccaggag gtaggtgggc aggtatgatg cttgagaaaa gattttggat
     1921 agtaatggtg aatccttaaa ataactcccg tcaacactaa ttgatttttg ctgcacaatg
     1981 gtggctgaaa ttgattttga ttcctctttt gctagctgca ccacgtaggg atgcgataat
     2041 aaattcagaa catccttgtt gtagaatttc acttcggtgc cggcggtttt tagattcgcc
     2101 tgaagcgaaa taagcccatc ggccagacta taggcaaaag tatcgtttat gggatagccc
     2161 atggtgatat taacctcatc caggttggtg ggtagtgcag aaattacggg cagcaggagt
     2221 tgctcctcag gcagcacaat ggcagtgtta ataccaaggt tttcgtcatc ataacccagc
     2281 ttattcagaa tgtttgaaac aatttttgcc t
//
LOCUS       DAOOQX010000302         2311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_56939_length_2311_cov_2.555851, whole genome shotgun sequence.
ACCESSION   DAOOQX010000302 DAOOQX010000000
VERSION     DAOOQX010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56939_length_2311_cov_2.555851"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /gene="fbp"
                     /locus_tag="PLX53_05595"
     CDS             complement(<1..760)
                     /gene="fbp"
                     /locus_tag="PLX53_05595"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOY72591.1"
                     /translation="MNGYNVKTLTQFIIERQADFPYATGEFTRLLYQVGVAAKLVNKK
                     VNKAGLVDVLGEAGNINVQGEEQKKLDLFANWQFIQALRNSRECCGIVSEEDQEIITW
                     DDEFSRDGNYLLCMDPLDGSSNIDVNVSIGTIFSIYRRISPRGEKPTLEDFLQPGNKQ
                     AAAGYVIYGSSTMLVYTTGKGVNGFTLDPSIGEFCLSHPKMKIPDDGVIYSINEGNYV
                     HFPEGVKKYLKYCQVEDEQTSRPYTSRYIGSMVAD"
     gene            959..2239
                     /locus_tag="PLX53_05600"
     CDS             959..2239
                     /locus_tag="PLX53_05600"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOY72592.1"
                     /translation="MSALRVFKFGGASVKSAEGIRNMAEIIRRQTSGNLVVVVSAIGK
                     TTNALEEILSLYLAESDEKANKLDQLKVFHTHIATELVGDSSSSHPLLAKVEQLFTEL
                     DEKMKFPPSGGYDYCYDQVVSYGELLSTTMVSFYLNQAGIINRWIDIRKCLRSDTSYR
                     EGVVDFELSQYLCEKEFCFDSDHLFITQGFIAGTAEGTTTTLGREGSDYTAAVLANLL
                     DATDVTIWKDVDGVLSADPKIFSNTCKLDFISYQEAIELAYCGAQVIHPKTIKPIQNK
                     QIPLYVKSFINPEASGTTIGHSDVPLSLPPVLILKRNQALVSLTPHDFSFVIEESLSK
                     IFAILYRHRVKVNLVQNSAISFTICVDDEHLRLPGAIEELQKTFTVRFNSNLELLTIR
                     HYTSHVVDEMVGERIVYLQQRTRSNARFIMDARL"
BASE COUNT          666 a          529 c          494 g          622 t
ORIGIN      
        1 catctgcaac catagaacca atatatcgcg aagtgtatgg acgtgaagtt tgttcgtcct
       61 caacctggca gtatttcaaa tatttcttca ctccttccgg aaaatgaaca taatttcctt
      121 catttattga ataaataaca ccatcatcag gaattttcat tttgggatgt gaaagacaaa
      181 attcaccgat agaaggatca agtgtaaatc catttacccc cttgcctgtt gtataaacca
      241 gcatggttga agaaccgtaa atcacgtaac cagcggcagc ctgcttgttg ccaggttgaa
      301 ggaaatcttc tagtgtaggt ttctctcccc gggggctgat acgtcgataa atgctgaata
      361 ttgttccaat ggaaacgtta acatcaatat tggatgagcc atcaagagga tccatacaga
      421 gcaggtagtt gccatcgcgt gagaactcat cgtcccatgt tataatctcc tggtcctcct
      481 ccgaaacaat tccacaacac tctcggctgt ttctgagagc ttgtatgaac tgccagttgg
      541 caaacaggtc gagtttcttc tgctcctctc cctgcacgtt gatgttgcca gcttcgccca
      601 gcacatccac gagcccagcc ttattaacct ttttgtttac aagctttgca gctacaccta
      661 cctggtaaag cagcctggtg aattcgccag ttgcataggg gaaatccgct tgccgctcaa
      721 tgatgaactg cgtgagcgtt tttacattat atccattcat ggggcagaaa aatgctactg
      781 attaacgttg ataaagataa ccaatttacc caaatcgaaa catgttaagt aacacaattg
      841 ccaaagttaa aacgtttgcg gaagatatac aagaacgtta cacttgaaag ccgggattat
      901 atcgataagt tgcccaaatc gaatcaaaca tgtatgtttg aataaatttt taaagaatat
      961 gtcggcattg cgtgttttca aatttggagg cgcctcggtg aaatcggcag aagggataag
     1021 gaatatggcc gaaattatcc gtcggcaaac atcgggaaat ctcgtagtgg tggtttcggc
     1081 catagggaaa acaaccaacg cattagagga gattctttcc ctgtatttag cagaaagcga
     1141 cgaaaaagct aataagttgg atcagctaaa agtttttcat acccacatag ccacggagct
     1201 ggtaggcgac agcagctctt cacatcctct attagccaag gttgaacagc tattcaccga
     1261 gcttgatgag aagatgaaat ttcctccaag tgggggttac gactactgct atgaccaggt
     1321 tgtatcgtat ggagagcttc tgtcaaccac catggttagc ttttacctaa accaagctgg
     1381 cataataaat aggtggattg acattcggaa gtgcttacgt tctgatacat cgtaccggga
     1441 gggggtggtt gatttcgagc tatcacagta tctctgcgaa aaagagtttt gctttgactc
     1501 cgaccattta tttatcaccc agggttttat tgccggaact gccgaaggca caactacaac
     1561 gctaggacga gagggatcgg attacacggc tgccgtgcta gccaacctgc tcgatgcaac
     1621 cgatgtaact atatggaagg atgttgacgg agtgctgagc gctgatccaa aaatcttttc
     1681 caacacctgt aaacttgatt ttatatccta ccaggaggcc attgagctgg cctactgcgg
     1741 agcccaggta attcatccca aaaccataaa acccattcag aataagcaaa ttcccttgta
     1801 tgtaaaatcg tttatcaacc ccgaagcttc gggtacaacc attggccact ctgatgtccc
     1861 attgagctta ccccccgtgc taattctaaa gcgcaatcaa gcattggtat cgctcactcc
     1921 acacgacttt tcctttgtca ttgaggaaag cctcagcaaa atattcgcca ttctttaccg
     1981 gcaccgggtt aaggtgaact tagttcaaaa ttcggctatc agcttcacca tttgtgttga
     2041 cgatgagcat ttacgcctac caggagccat agaagaactt caaaaaacct tcacggttcg
     2101 attcaacagc aaccttgaac tgctcaccat taggcactat accagccatg tagtagatga
     2161 aatggttggc gaaaggatag tttacctcca gcagcgtacg cgaagtaatg ccaggtttat
     2221 tatggatgca cgtttgtagg tttaactaca aatacagggc tatagcccaa aaacgtattc
     2281 tggttgctga acgcttctag ccccagcgtg t
//
LOCUS       DAOOQX010000303         2307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_57101_length_2307_cov_2.712256, whole genome shotgun sequence.
ACCESSION   DAOOQX010000303 DAOOQX010000000
VERSION     DAOOQX010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57101_length_2307_cov_2.712256"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLX53_05605"
     CDS             complement(<1..649)
                     /locus_tag="PLX53_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOY72593.1"
                     /translation="MDIFDKIDNMGPIGQWQSQSHGYFSFPKLEGEIEPHMIFRGKRV
                     LNWSLNNYLGLANLPEVRKADAEAAAQYGLAYPMGARMMSGQTRKHEELEEELARFVG
                     KPSAYVLNYGYQGMVSIIDTLLGRNDVVIYDSESHACIIDGLRIFPGKRFVYQHNDME
                     SCRKQLEHATAVVEKTGGGILLVTEGVFGMAGDLGNLKAITDLKKEFNFRILVDDA"
     gene            1223..2035
                     /gene="bamD"
                     /locus_tag="PLX53_05610"
     CDS             1223..2035
                     /gene="bamD"
                     /locus_tag="PLX53_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOY72594.1"
                     /translation="MTKHRDTKGILFLFTILATIFMVSCSGYEKLLKSKDYEYKYKKA
                     LEYYEKKEHYRYTTLLEQLSPIYKGTFRSDTIEYYIAQGYYYQGDYLLAGHSYDKFRQ
                     TFQRSAFTEDAEFMYAYCFYKSSPRPELDQASTQEAIEAFVEFLTRFPNTNRRTEVNG
                     LLTELREKLVEKSYLSSKLYYKIGDYKAAIVAIQNSLKDFPNSKYREELSYLLVKSSY
                     LLAENSIPNKQRERYQNTIDQYYTFVGEFPESPYLPEAKKMYESSMKVLQIN"
     gene            2053..>2307
                     /locus_tag="PLX53_05615"
     CDS             2053..>2307
                     /locus_tag="PLX53_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOY72595.1"
                     /translation="MDLKKMNVPASTITRNLDELDKETGNIYQTVRIIAKRANKLSVD
                     IKQELNHKLEEFAYYTDNLEEVFENREQIEISKYYEKLPKP"
BASE COUNT          733 a          546 c          440 g          588 t
ORIGIN      
        1 gagcatcgtc aaccagaatg cgaaagttaa actccttttt taggtcagta atagccttca
       61 ggttaccaag gtcaccggcc atcccgaata cgccttctgt aactagcaag atgccgcccc
      121 ctgttttctc aacaacagct gtagcatgtt ccagctgctt acggcagctt tccatgtcgt
      181 tatgctggta aacaaaacgt ttgccgggga atattctaag cccgtcaata atgcaggcat
      241 gggattcgga atcgtaaata accacatcgt tcctgccaag cagcgtatct ataatagaaa
      301 ccatcccctg ataaccatag ttgagtacgt aggcagaggg ctttcctaca aacctggcaa
      361 gttcctcctc cagctcctcg tgcttacggg tttgaccact catcatccgg gcacccatgg
      421 ggtaagccaa cccatactgc gcagcagcct cggcatctgc ctttctaact tctggcaggt
      481 ttgcaagccc caggtagttg tttaggctcc agtttagcac ccttttgccc ctaaaaatca
      541 tgtgcggttc aatttctcct tccaacttcg ggaaagaaaa atacccgtgg ctctggcttt
      601 gccattgccc aatgggcccc atgttatcaa ttttatcaaa aatatccact gctgtaaatt
      661 ttaaaagtta agatggaatg aataaagtgt taaaatttag ccgcaaatct agctcaattt
      721 tattaaaaaa acgtccttta tttcaatatt gaaaaaattg aaacatcccg taaaaagatt
      781 tataaagcca gctaacttgt atgtagagta aagattatca gcagcataca aacagaaagc
      841 gctcaccaac agcaaacttc tgcctgaggg aaaccgtaag catcagctgg ttccatcacc
      901 taaactaccg atcttacaaa agaataccaa acaagttttt caaacatttg atcggaaaag
      961 tagctgccct acgacccaca cagtaaaata tcaaagaaag atataacctt tcttacccaa
     1021 aaatcctcga taccctgcaa ggttaattaa ccttttttag atgtacagct tgtttcaagt
     1081 tttaatttac cgctaagttt tataactttg cctgctattt tatgctactg caagctgcaa
     1141 aacagcctac gaaaaagggc tcaaatagca gtttgtttaa ttttcagcta aagagagtaa
     1201 gcagcattaa acaagcccta taatgacaaa acacagagat accaagggaa ttttatttct
     1261 ttttacaatt cttgccacca ttttcatggt ttcttgcagc ggttatgaaa aactcctgaa
     1321 gagtaaggat tacgagtata agtacaaaaa agcactggag tactacgaaa aaaaagaaca
     1381 ttaccgctac accacgctgc tggagcagct atcgcccatc tacaagggaa cctttagatc
     1441 ggatactata gaatactaca ttgcacaagg ctactactac cagggcgact acctgcttgc
     1501 tggtcatagc tatgacaagt ttaggcagac atttcaacga agcgctttca ccgaggatgc
     1561 cgagttcatg tatgcctact gcttctataa atcttcaccc cgtccggaac tcgaccaggc
     1621 cagtacccag gaagcaattg aggctttcgt ggaatttctc acccgctttc ctaataccaa
     1681 ccgtaggacc gaggttaacg ggttgctcac cgagctcagg gaaaaattgg tggaaaagtc
     1741 ctacctcagc tctaagctat actataaaat tggcgattat aaggctgcaa tcgtcgccat
     1801 tcagaacagc ttgaaggatt ttccaaacag taagtaccgt gaagagctat cctacctgct
     1861 ggttaaatcg tcgtacctgc ttgccgaaaa cagcattccc aataagcaaa gggaacgcta
     1921 ccaaaatacc attgaccagt actatacctt tgttggcgag tttccggaat caccctactt
     1981 gcccgaggct aaaaagatgt atgagagctc aatgaaagtt ttgcaaatca actaacaatc
     2041 aatagtaaac ccatggattt aaaaaaaatg aacgttccgg cttcgaccat cacgcgcaac
     2101 cttgacgagc tagataagga aaccgggaat atttaccaaa cggtcagaat tattgccaaa
     2161 agggctaaca agctgagcgt tgacattaag caggagctaa accataagct cgaggagttt
     2221 gcctactaca ccgataacct tgaagaggtg tttgaaaaca gggagcaaat agaaatttca
     2281 aagtactacg agaagctgcc taaacca
//
LOCUS       DAOOQX010000304         2304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_57216_length_2304_cov_2.415740, whole genome shotgun sequence.
ACCESSION   DAOOQX010000304 DAOOQX010000000
VERSION     DAOOQX010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57216_length_2304_cov_2.415740"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PLX53_05620"
     CDS             <1..754
                     /locus_tag="PLX53_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOY72596.1"
                     /translation="KNAPAAKLTQIVMYGASIVPVDGSYDQAFDLSVKSSETFGWYNR
                     NTAFNPLTIEGKKTVSFEIFEQLKGQLPDRIFVPVGDGVIISGVYKGFEDLLMLKLID
                     RMPTIVAVQAKGSSNLVGNLFTDVFCSQTSQTLADSISVDIPRNFYMARQYIKTYNGE
                     AVTVTDGEILEASAILARNTGLFAEPAASAALAGLISFFRQNKVEGNTSNMVLLTGSG
                     LKDLKAVSPIVQFPKAIKPKIDALKELVDTWK"
     gene            890..>2304
                     /locus_tag="PLX53_05625"
     CDS             890..>2304
                     /locus_tag="PLX53_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOY72597.1"
                     /translation="MITFTLNSKIVEFTGDPKSNLLVFLRNTMHLTAAKDGCSGQGVC
                     GACSVEVDGKIKMACRTKMGDIEGSVVFTLEGLDETFRKTLGRNFALRGAVQCGFCSP
                     GMIMRAKGLYNQNPKPSRNEIIRAITPNLCRCTGYVKIIDAIDASFKELAGELEPTVV
                     AEAKIGERYPKYQAEATALGERPFVDDLRIDGMLFGALKFSDHPRAEILSIDVAPAMS
                     IDGVVGIYTAKDIPGNHFSGLVFQDWPLMVGKGETTHYIGDVVASVVATTEETARKAV
                     EQIRVTYRVFSPVTDIHQAILPNSPNVHTERSNVLETCTIRFGEVKKAFEEAAYISSD
                     VYETQRIEHAFLETETALALPDGDGVKLYSQGQGVYIDRSQIAKFLDIPEEKVKVVQV
                     QNGGGFGGKEDLTVQGHAALCAYLSGMPIKIHLNREESLRMHPKRHPVWMKMSLACNS
                     KGKFTALKLDAIGDTGAYASVG"
BASE COUNT          671 a          482 c          554 g          597 t
ORIGIN      
        1 caaaaatgct cctgcagcca agttgacgca aatagtaatg tatggcgcat caattgttcc
       61 tgttgatgga tcctacgatc aagctttcga cttgagtgtg aaatcatccg aaacatttgg
      121 ctggtacaac aggaataccg cttttaaccc gctaaccatt gaggggaaaa agaccgtttc
      181 gttcgaaatc tttgagcagc tgaagggcca gctgcccgat agaatttttg ttcccgttgg
      241 cgatggcgtt atcatctcag gtgtttacaa gggatttgag gatcttctga tgttgaagtt
      301 gattgacaga atgcctacca tcgttgccgt tcaggctaaa ggtagtagca atcttgttgg
      361 taatcttttc accgatgttt tttgttcgca aaccagccaa accctggctg attccatttc
      421 ggtagatatt ccccgtaact tctacatggc ccgtcaatac attaaaacat acaatggcga
      481 agctgtaacg gttactgatg gtgaaattct tgaagcctca gccattctgg caagaaatac
      541 cggactcttt gccgaacctg ctgcttcggc agcacttgca ggcctaatat cgttcttcag
      601 gcaaaataaa gttgaaggga atacctcaaa catggttctg ttaaccggca gcggtctgaa
      661 agacctaaaa gccgtttcgc ccattgttca atttccaaaa gccattaaac caaaaattga
      721 tgctcttaag gaacttgttg atacctggaa atgaatagtt taagccaaaa tagtgcattt
      781 taaccaaaat caaccagcta ataggcaaac gcaatccctt caaattagta aattgcagct
      841 tgttcctgga taagacagct attcgagttc aatcatcttc taaaaatcta tgataacttt
      901 tacgctaaac tccaaaattg ttgagtttac aggagaccca aaaagcaacc tgctggtttt
      961 tcttcgaaat acaatgcacc ttactgcagc caaagatgga tgttccggac agggtgtatg
     1021 tggagcatgt tcagttgaag ttgacggtaa gattaaaatg gcctgccgaa caaaaatggg
     1081 agacattgag ggatcggttg tcttcaccct cgaggggctc gatgaaacat ttaggaaaac
     1141 gttgggtcga aattttgccc tcaggggtgc cgttcagtgt ggtttctgct cacctggtat
     1201 gattatgagg gcaaaggggc tttacaacca aaacccaaaa ccctccagga acgagattat
     1261 aagagcaata acccctaacc tttgcaggtg taccggatac gttaaaatta ttgatgctat
     1321 agacgcctca tttaaagaac ttgcaggcga gttagaacct actgtcgttg ccgaagcaaa
     1381 aattggagaa cgctatccaa agtaccaggc cgaagcaaca gctctgggcg agaggccctt
     1441 tgttgacgac ttacggatag acggaatgct ttttggcgca ttgaaattct ccgaccatcc
     1501 acgtgccgag atactcagca ttgatgttgc ccctgctatg agcattgatg gggtggtagg
     1561 aatatacact gcaaaggata taccgggtaa ccacttcagc gggttggtat ttcaggattg
     1621 gccgctgatg gttggtaaag gtgaaacaac acattacatt ggcgatgtag tagccagtgt
     1681 tgtagccaca actgaggaga cagcacgcaa agctgtagag caaatcaggg taacctacag
     1741 ggtttttagc ccggtaaccg atattcatca agccatactt cctaacagtc cgaatgttca
     1801 tactgaaagg tcaaacgtgc tggaaacctg cacaatacgt tttggtgagg tgaaaaaagc
     1861 attcgaggaa gcagcctaca tttcatcaga tgtctatgaa acacagcgca tagagcatgc
     1921 atttctggaa actgaaacag cgttggcatt acccgatggt gatggtgtaa agttgtatag
     1981 ccaggggcaa ggagtttaca tagacagaag tcaaattgcc aaatttcttg atattcccga
     2041 ggaaaaggta aaagttgttc aggttcagaa tggaggaggg tttggaggta aggaggacct
     2101 aaccgtgcag gggcatgccg ccctgtgtgc ctatctctcc ggaatgccga ttaagattca
     2161 tctgaatagg gaggaatcgc tgagaatgca tcctaagagg catcctgtgt ggatgaaaat
     2221 gagcttggct tgcaactcaa agggaaaatt cacggcgctt aaacttgatg ctattggcga
     2281 taccggagct tacgcctcgg tggg
//
LOCUS       DAOOQX010000305         2302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_57282_length_2302_cov_2.887850, whole genome shotgun sequence.
ACCESSION   DAOOQX010000305 DAOOQX010000000
VERSION     DAOOQX010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57282_length_2302_cov_2.887850"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLX53_05630"
     CDS             complement(<1..619)
                     /locus_tag="PLX53_05630"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318324.1"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOY72598.1"
                     /translation="MSGVNVLIIGSGGREHALAWKLSQSPKLGKLFALPGNPGTARLG
                     ENIPLSVLDFEGIRTVVLEKNINLVVIGPEEPLVKGLYNFLTGDLGLRNVLVVGPGRE
                     GAMLEGSKNFAKAFMQRHGIPTARYRAFTRDEYSEAKKFLGTLSSPYVLKADGLAAGK
                     GVVIQASYEDACASLGEFFSGKFGDAGNVVVIEEYLRGVELSVFVL"
     gene            820..1305
                     /locus_tag="PLX53_05635"
     CDS             820..1305
                     /locus_tag="PLX53_05635"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOY72599.1"
                     /translation="MVISIVVATSENRVIGKNNQLLWHISEDLKRFKKLTTGHTVIMG
                     RKTFESIGQPLPNRHNIVISRSEGLKFEGCTAAKSLADALEIASTDEEVFIIGGGEVY
                     RQALPLAERIYLTLVHANYEGDTFFPALNPNEWKVVREEKVSPTEGPGISFLDYVRQH
                     S"
     gene            complement(1302..>2302)
                     /gene="clpX"
                     /locus_tag="PLX53_05640"
     CDS             complement(1302..>2302)
                     /gene="clpX"
                     /locus_tag="PLX53_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOY72600.1"
                     /translation="EAKKYLSVAVYNHYKRLLQKDMGDDVEIEKSNILLVGETGTGKT
                     LLARTIARMLHVPFTIVDATVLTEAGYVGEDVESILTRLLQVADYNVEAAEKGIVFID
                     EIDKIARKGDNPSITRDVSGEGVQQALLKLLEGSVVNVPPQGGRKHPEQKMIAVDTKN
                     ILFICGGAFEGIEKKIAQRLNTTVVGYGAGKTREVIDKNNLLQYIAPQDLRAYGLIPE
                     IIGRLPVLTFLSPLDKVALRKILTEPKNAIIKQYIKLFEYDGIKLQFEEEVLDYIVNK
                     AVEFKLGARGLRSICEAIMMDAMFDLPTGSEKEITVNLDYAQQKLEKSNLKRLKAA"
BASE COUNT          617 a          590 c          469 g          626 t
ORIGIN      
        1 taagaacaaa aaccgatagc tccacccccc taagatactc ctcgataacc accacgttcc
       61 cagcatcacc aaattttccg ctgaaaaatt cgccaaggct cgcacacgca tcttcatatg
      121 aggcttggat aaccactccc ttaccggcag ccaacccatc ggctttcaaa acataaggag
      181 aggaaagagt tcccagaaat tttttggctt cgctgtattc atccctggtg aatgctctgt
      241 accttgcagt tggaattcca tggcgctgca tgaacgcctt ggcaaagttc ttgctgccct
      301 caagcatggc accttccctg ccgggtccaa ccaccaaaac atttctaagc cctaaatcac
      361 ccgttagaaa attatacaac ccctttacca atggctcctc aggaccaata actaccaggt
      421 taatgttttt ttcgagaact actgtcctta tcccttcaaa atcgagcaca ctaaggggta
      481 tattttcacc caaccgggca gttccgggat ttcctggtag agcaaatagc tttcctaact
      541 ttgggctttg tgaaagtttc catgccagcg catgctccct ccctccagag ccaatgatta
      601 acacgtttac tcccgacatt ttatagatgt ttgctacaaa ggtagcaatc atttgggttg
      661 acacaacaac gtaaaccaca tctgcatttc attgccaaag ccaatgtcca gaatgtttta
      721 acttttttac aaccgttaaa gtgctcaaca ggaatcaact cttgcaaact acccataatt
      781 gatgctaact ttaccgctac aattctaaaa taattccgaa tggttatctc catagttgtt
      841 gcaacttcag aaaacagggt aattggtaaa aacaaccagc tgctttggca tatttctgaa
      901 gatttaaaac gttttaagaa gctaacaacc ggacatacgg tgataatggg ccgtaaaaca
      961 tttgaaagta taggccagcc actaccaaac cggcataata tcgtaatttc acgtagcgag
     1021 ggcttgaagt ttgagggttg cacagctgca aaatcgttgg ccgacgccct ggagatagcc
     1081 tcaacagatg aagaggtttt tattattggc ggcggggagg tttaccggca ggcattgccc
     1141 cttgccgaaa gaatatacct aacgttggta catgctaact atgagggtga tacgtttttc
     1201 cccgcattaa acccgaacga gtggaaagtt gtaagggaag aaaaagtaag cccgaccgaa
     1261 gggccgggta tctccttttt agattatgtt cggcagcact cctaagcagc cttaagccgc
     1321 ttcaggttcg atttttcgag tttttgctgt gcgtaatcca ggttaacagt aatctccttt
     1381 tcactccctg ttggcaaatc gaacatggca tccatcataa tggcctcaca aatagaccta
     1441 agtccccttg cccccagctt aaactctacg gccttgttga caatgtagtc tagcacctcc
     1501 tcttcgaact gcaactttat gccatcgtac tcaaaaagct tgatgtactg cttgatgatg
     1561 gcatttttgg gttcggtgag aattttccta agggcaacct tgtccaacgg gctaaggaag
     1621 gtgagcaccg gcaggcgccc aatgatttcg ggtataagac cataggctcg tagatcctgc
     1681 ggagcaatgt actgtagcag gttgttttta tcaatgacct cacgggtttt ccctgcaccg
     1741 tatccaacca ccgtggtatt gagcctttgg gcaatttttt tctcaattcc ctcgaaggct
     1801 cccccgcata tgaaaagaat gtttttagta tcaacggcta tcatcttctg ctcgggatgc
     1861 tttctgccac cttgtggcgg aacgttgaca acagagccct caagaagctt gagcagtgcc
     1921 tgctgaactc cctctcctga tacatcacgg gtaattgacg gattatcgcc cttacgggca
     1981 attttatcaa tttcatcaat aaacacaatg cccttttcgg ctgcctcaac gttgtagtcg
     2041 gcaacctgaa gcaaacgagt aagaatgctt tccacatcct ctcccacgta gcctgcctcg
     2101 gttagcactg tagcatcaac tattgtgaaa ggaacatgca gcatacgggc aatagtacgg
     2161 gccaaaagtg ttttgcctgt tcctgtttcg cctaccagca gaatattcga tttttcaatt
     2221 tcaacatcat cacccatatc cttctgcagg aggcgcttgt agtggttgta gaccgccacc
     2281 gacaggtact tcttggcctc at
//
LOCUS       DAOOQX010000306         2300 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_57370_length_2300_cov_2.996882, whole genome shotgun sequence.
ACCESSION   DAOOQX010000306 DAOOQX010000000
VERSION     DAOOQX010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57370_length_2300_cov_2.996882"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PLX53_05645"
     CDS             complement(<1..503)
                     /locus_tag="PLX53_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72601.1"
                     /translation="MIKIVDCVSNAKALENAVGRFREKGIILPTIKQQQNPELVPSKI
                     QEQLRGIGLWEINPLNLFRITWKNEPKEKGGLFGGVNFIELPSELTGVKARIVMLIGK
                     WFPTGAHKVGAAYGCLAPQITTGGFDPTTQKAVWPSTGNYCRGGAFDSKLMGADSVAI
                     LPQEMSS"
     gene            770..1231
                     /locus_tag="PLX53_05650"
     CDS             770..1231
                     /locus_tag="PLX53_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOY72602.1"
                     /translation="MKNIDEIDGKLLNILQDNSRVTIRELSEKLHLSTTPIHERIKKL
                     EKSGIIKQYVTLLDPKILGKKLTVFISVSLSSHVKEVIDEFEREIKVMPEVMECYYVS
                     GTWDFFIKIQCTDMDDYHNFVINRFSRLKNITQFYSSFVMSESKVSYKHQL"
     gene            complement(1232..1588)
                     /locus_tag="PLX53_05655"
     CDS             complement(1232..1588)
                     /locus_tag="PLX53_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72603.1"
                     /translation="MLGVGRVAVESLANGLNVISVNCKRMGSIVNLRNYSAYRLNNFV
                     DINGIPPTRESLLSEINKFFQNRVQYKDESILIREKVIQEFNIKTITLQTMNVYSEVT
                     QKQLQKNVTSSSSHTC"
     gene            complement(1681..>2300)
                     /locus_tag="PLX53_05660"
     CDS             complement(1681..>2300)
                     /locus_tag="PLX53_05660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOY72604.1"
                     /translation="FGGAENHVRELSNELAAQAHDVYLVGPSGKQIKLLNQKVHFVQA
                     SQLSKLLVFQTFFLIYLIKKHQIEVIHAHQRLSILAACWAGFITGTPVVATVHGRVRF
                     DLRSSFSRTRTSKIIFVSHQVLKVSKYYSEINHKSVVIPNGINFQKIIPKRIPYGMAY
                     ISRMDSHHKEVVDMLIGLMPDVTASFPQASLWIFGEGKQVEYLKK"
BASE COUNT          659 a          460 c          466 g          715 t
ORIGIN      
        1 tcgctgctca tctcctgtgg aaggatggct actgaatcgg cacccatcag cttggagtcg
       61 aatgctccac cacggcagta gttgcctgtg gatggccaaa ctgctttttg tgtggtaggg
      121 tcaaaacccc cggttgttat ttggggggcc aagcagccat aggcagcacc taccttatgt
      181 gcacccgtgg ggaaccactt accgataagc ataactattc gagccttcac gccggtaagc
      241 tccgaaggca gctcaataaa gttaaccccg ccgaagagcc cacccttctc tttcggttcg
      301 tttttccaag taatcctgaa aagattcaag gggtttattt cccataaccc aatacctctt
      361 agctgctctt gaatctttga tggcactaac tcggggtttt gctgctgctt gatcgttggg
      421 agaatgatgc ccttttctct gaaacggcct acagcatttt ccaacgcttt ggcatttgaa
      481 acgcaatcaa ctatttttat cataatatgt aaaaaaaata ttttcccaaa attagtattg
      541 gtataataaa tgactattaa tattgataaa aataagaaat aaagttaata aataattaaa
      601 tattgagaaa attgatattt ttgtatggag attttggaga agtcataggc ttttttgatt
      661 atcgatcatg ttagccttaa acttaaacga agccaacttt aatttgcagc caagtgtgcc
      721 tagtaagtaa tgtttactga atataaatca tataaattta ttgattccca tgaagaatat
      781 tgatgaaata gacggcaagt tgctaaacat actccaggac aacagcaggg taaccatcag
      841 ggagctatcc gagaagcttc atctttctac cactcctatt catgagcgaa ttaaaaagct
      901 tgaaaaatca ggtattatca agcagtatgt aacactgctc gatcccaaaa ttcttgggaa
      961 aaagctaaca gtattcattt cggtgtcgct ttcaagccat gtaaaggagg ttatagacga
     1021 gtttgagagg gaaattaaag tgatgcccga ggtaatggag tgctactacg tttcaggaac
     1081 ttgggatttt tttatcaaaa ttcaatgtac cgatatggac gactaccata actttgtcat
     1141 aaaccgattt tcgaggttaa agaatatcac gcaattttac agctcgtttg taatgtccga
     1201 gtcgaaagta tcgtataagc accagctgta gctagcatgt gtggctactg ctagatgtaa
     1261 cgtttttttg cagttgcttt tgagttacct cggagtaaac attcatagtt tgaagggtaa
     1321 ttgtttttat gttaaattcc tgaattacct tttctcttat gaggatggac tcatccttat
     1381 actgaactcg attttgaaag aatttattga tttctgacag aagcgattcc cgggtgggtg
     1441 gaataccgtt tatatcaaca aaattattca aacggtatgc cgaatagttc cgtaggttaa
     1501 ctatactgcc cattctttta cagttcactg aaattacgtt gaggccgtta gccagcgatt
     1561 ctacagctac tcttccaacc cctagcacca gctcgggtaa aatttcctgc tggttgaaat
     1621 tctcaacgta cccgcatatt cttacaacct ctctatgtag gctctggttt agttgggatg
     1681 ctactttttt aaatattcaa cctgctttcc ttcaccgaat atccataagc ttgcctgagg
     1741 gaagctggct gtaacatcgg gcattaatcc aataagcatg tcaacaacct ctttgtgatg
     1801 gctatccatc cggctgatgt atgccattcc ataaggaata cgctttggta taattttttg
     1861 aaagtttatc ccatttggaa tcacaaccga tttgtggttg atttcagagt aatattttga
     1921 taccttcagt acttgatggc ttacaaaaat aatttttgag gttctcgtgc gtgaaaacga
     1981 tgagcgaagg tcgaatctaa ctctgccatg aacagtggct actacgggtg ttcctgttat
     2041 gaatcctgcc caacaggcag ctaaaatgga tagacgctga tgggcatgaa ttacctcaat
     2101 ctgatgtttt tttataaggt aaatcaaaaa aaaggtttgg aataccagca gcttacttag
     2161 ctggcttgct tgaacgaagt gaactttttg gtttaaaagc ttgatctgct ttcctgatgg
     2221 tccaaccagg taaacgtcgt gagcctgagc tgccagctca ttgcttagct ctcgaacgtg
     2281 attttcggca cctccaaaat
//
LOCUS       DAOOQX010000307         2299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_57399_length_2299_cov_3.549911, whole genome shotgun sequence.
ACCESSION   DAOOQX010000307 DAOOQX010000000
VERSION     DAOOQX010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57399_length_2299_cov_3.549911"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..>2299)
                     /locus_tag="PLX53_05665"
     CDS             complement(197..>2299)
                     /locus_tag="PLX53_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY72605.1"
                     /translation="VFGRNCHGAGDAIRIGEGTSAVLRGKQVAYEILQELDVRFNYDN
                     FLDISKEYIDSQQHPVRVIDKPFIPDEERRRSKPFVQIDCLYGFACNPCTFACPQGAI
                     TKSSTSAVPQIDYTKCTGCFDCVYQCPGLAIFGYSYTKDWLFLPIEYEAQEGAEVFLV
                     DNNGKKLGEGIIEKILRKPNKTNVARVKSLSLHGDDLLNARGFIVKSSYPQPLDTKPV
                     SHKSEEKIYVCHCDDVTLDEVLETIGDRKFISADEIKHTTRLGMGACRGKRCIKRLKT
                     TLAPLGIQIVGDATPRGPLSNQLTLGELYPKDVHETYITGVNGKKVRVEKVSALVAGG
                     GMGGSALFRYMAEAGLKPILLNFGRGSSWRNIAGGRTAFSLPEIADIAKVNHEIFREL
                     HGISSIDYRPINYVNFAHDDSTLKALEVSMAWSDAYMVEPKDFSKEISPYMNPSLATY
                     KAAQITRGCWQATPGKTVDLLRKIGIRNGGTVKEDCELISVYRDGAGYVALVRDHDHS
                     YIEFHADSFVNALGPSGEKFNRQLGIYTGLYPVKHQAFITRRLPWLGINNAPLGMLID
                     RRNYKGFVAVYGQQLAETGQIIGCASPAVDAREADKNLKVNSQEFLEIASEVFSSWIP
                     MLGTVGFQAVWAGYYTEPRMFIDPKHGLFTGLRGQGFMLGMGLAKMYVDALTGKPVPD
                     YFGRLSLEGDGLPEKAFK"
BASE COUNT          528 a          578 c          572 g          621 t
ORIGIN      
        1 aaaatttcaa tgttgtagct aaaaattaac ttcgttgagc tcgcaagctc ggttcatttt
       61 ttgaaaaaat tcctaactat caatcaatca gtcattaaga caacacttaa aaagttgcat
      121 tagttacttg aatttatgga aaaaaccctg ccagcaaagg ttggcagggc ttttgagtga
      181 agtggagtat ccagctttac ttgaatgctt tctctggcag gccgtcgcct tcgaggctta
      241 agcggccaaa gtagtcggga acaggttttc ccgtaagcgc atcaacgtac attttggcta
      301 gccccatgcc tagcatgaat ccctggcctc gtaagcctgt aaaaagaccg tgtttggggt
      361 caatgaacat tctaggctcg gtgtagtaac ctgcccaaac agcctgaaat cctactgttc
      421 ctaacatcgg tatccagctg gagaacacct cggaggcaat ttccagaaat tcctgggagt
      481 taaccttcag gtttttgtca gcctcgcggg catcaactgc cggggaggcg cacccaatta
      541 tctgtccggt ttcggccagc tgctgaccat aaacagcaac aaatcccttg tagttccttc
      601 tatctataag cattcctagt ggtgcgttgt ttataccaag ccagggaagc ctacgggtaa
      661 tgaatgcctg atgcttaacc ggatataggc cggtgtaaat gcccagctgc cggttgaatt
      721 tttcacccga cgggccaagt gcattcacaa agctatcggc atggaactca atgtagctgt
      781 ggtcgtggtc acgcacaaga gctacgtatc ctgctccatc gcggtaaacg ctaatcagct
      841 cgcagtcctc cttaaccgtt cctccattcc tgattccaat cttacggagt aggtcaaccg
      901 ttttgccagg ggtagcttgc cagcagccac gggtaatctg tgcggctttg taggtggcaa
      961 gcgaagggtt catgtagggt gaaatttcct tgctgaaatc ctttggttcc accatgtagg
     1021 catcgctcca ggccatggag acctccagtg cttttagcgt gctatcgtcg tgggcaaagt
     1081 tcacgtagtt tatagggcgg tagtcaatac tggaaatgcc atgcagctcc ctgaatattt
     1141 catggtttac cttggcaatg tcggcaattt caggaaggga gaatgctgtc cttccaccgg
     1201 caatgtttcg ccagctggag cctctaccaa aattaagcag gatgggtttt aacccggcct
     1261 cggccatgta gcggaacaag gcgcttcctc ccattcctcc gccggcaacc agggcagaaa
     1321 ccttttcaac cctaactttt tttccgttta ctccggtgat gtaggtttcg tgaacgtcct
     1381 ttgggtagag ctctccaagt gtgagctggt tgctaagcgg cccacggggt gtggcatcgc
     1441 ccacaatctg aatgcccagt ggagccaggg ttgtcttcag ccttttaata cacctttttc
     1501 cccggcaggc gcccatgccc aggcgggtgg tatgcttaat ttcatcggct gaaataaact
     1561 ttcggtcgcc gatagtttcc agcacctcgt caagcgtaac atcatcgcag tggcaaacgt
     1621 agattttttc ctctgactta tgcgaaaccg gtttggtatc gaggggttgg ggatagctgc
     1681 tcttcactat aaatccccga gcgttgagta ggtcgtcacc gtgaagtgaa agcgatttca
     1741 ccctggccac gttagttttg ttgggttttc ggagaatctt ttcaatgata ccctcgccaa
     1801 gtttcttgcc gttgttatca accaggaaaa cctcagcgcc ttcctgggcc tcgtactcta
     1861 tgggcaggaa cagccaatcc ttggtgtagc tgtaaccgaa tattgccagg ccggggcact
     1921 ggtaaacgca atcgaagcag ccggtgcact tggtgtagtc aatttgggga acagcgctgg
     1981 ttgaactttt cgttatggct ccctgtgggc aggcaaatgt gcatgggttg catgcaaacc
     2041 catagaggca gtctatctga acaaagggtt tgctccttct ccgctcctca tcagggatga
     2101 aaggtttatc aattaccctt acagggtgtt gctggctatc aatatactcc ttgctgatgt
     2161 cgaggaagtt gtcgtagttg aaccttacat cgagctcctg gagtatttcg taggctacct
     2221 gcttgcctct aagaactgcg cttgttccct cgccaatgcg aatggcatcg ccggcgccat
     2281 ggcagttgcg tccgaacac
//
LOCUS       DAOOQX010000308         2293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_57620_length_2293_cov_2.739500, whole genome shotgun sequence.
ACCESSION   DAOOQX010000308 DAOOQX010000000
VERSION     DAOOQX010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57620_length_2293_cov_2.739500"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1979
                     /locus_tag="PLX53_05670"
     CDS             <1..1979
                     /locus_tag="PLX53_05670"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HOY72606.1"
                     /translation="NLALVEAQRDKSNEALGDAYNSMGNAYSSKHENETAITYYLKSK
                     DYREKSGDWTKVGHSLSNLGLTLRDLKRYSESISSYRQAAKYYNDAKNYLFEGISYLD
                     IAQVYRQLNDLNRALENAILAINIFLEHDITQPLARCYNFIGNLHKDLKNFDLAEDYY
                     LKAYEIWSKANNLSGISSALNNLGIIYDEKGNYEKALENYNRSLDIAIQLNDSIGIST
                     AYNNIGFIYVKLNQLERGINAYLKSMAISRQIGEIESYLNTCNNVASVYLKAGMLDKC
                     EEYLNIVLGNLKSITELAYIQETYQLLSELYAKRGNYKKAFEFKSLQLAYNDSIYNQQ
                     QVRNILETQTRFETEAKEREIQLLKKDNEIKQLEFEKQRNFQNILTFITILLAIAIAV
                     TIYISRIIRKKNLLLAEKNRALEEANKKMAETEVNLRELNATKDKLFSIIAHDLKNPF
                     NALLGFSDILERNYNFLSDEERIEYIGVINDSTQSLYKLLDNLLQWTRTQTGTITYIP
                     ENFKLLPLVKQEITNVNLNSEKKKISITVNIDENISVFADKNTTATIIRNLLSNAIKF
                     TDLGGHIEIAARYKDQMVETSVSDSGIGINDNDLDRIFMLDGSFTTKGTSNESGTGLG
                     LLLCKEFVEKNNGKIWVESEKGKGSTFFFTLPAK"
     gene            2085..>2293
                     /locus_tag="PLX53_05675"
     CDS             2085..>2293
                     /locus_tag="PLX53_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72607.1"
                     /translation="MKLVIQKWGVALLLTAYSLIAAQAGSPSSSKVDSLYQRVLAIAD
                     ENPQEASRLANLCRQESSKSGNSRD"
BASE COUNT          764 a          519 c          470 g          540 t
ORIGIN      
        1 ccaacctggc cttggttgaa gcccaacgag acaaaagcaa cgaagcgctg ggcgatgcat
       61 acaacagtat gggtaatgct tactcatcaa agcatgagaa cgaaacggcc attacctact
      121 accttaaatc gaaagactac cgtgagaaga gcggtgactg gacaaaggta ggccactcat
      181 taagcaatct aggattgacg ctacgagatc tcaagcgata cagcgagtcc atcagcagct
      241 acaggcaggc agcaaaatat tacaatgatg ctaagaatta cctctttgag ggaatttcat
      301 accttgacat agcccaggtt taccgccagc tcaacgacct taacagggct cttgaaaacg
      361 ccattttggc aataaatatt tttctggagc atgacatcac tcaacccctg gcccgatgct
      421 acaactttat tggaaacctg cataaagacc ttaagaattt cgatttggca gaggattact
      481 atcttaaagc ttatgaaatt tggtcaaaag cgaataacct atccggtatt tcatctgctt
      541 taaacaacct gggaataatt tacgacgaga aggggaacta cgaaaaggcc cttgaaaact
      601 acaaccgctc gctggatatt gccatacaac ttaacgattc tataggtatt tctacagcct
      661 acaacaatat tgggtttata tacgtgaagc tcaaccagct ggagcgaggc atcaacgcct
      721 acctgaaatc tatggctata tcaaggcaaa taggcgaaat tgagtcgtac cttaatactt
      781 gcaataatgt tgcctcggtt taccttaaag caggaatgct agacaagtgt gaggagtacc
      841 tgaatatagt tcttggcaat cttaaaagta taaccgagct ggcctacatt caggaaacct
      901 accagctgct cagtgagctg tatgccaagc gtggaaacta caaaaaggca ttcgagttta
      961 agtcgctgca gctggcatac aacgacagca tatacaacca gcaacaggtc cgaaacatac
     1021 tcgaaactca aaccaggttc gaaactgaag caaaggaacg tgagatacaa ctcttaaaaa
     1081 aggacaacga aataaagcag cttgagtttg agaagcagcg aaattttcaa aacattctaa
     1141 cgttcatcac catcctactg gccatagcaa ttgcggttac catatacatt tcacgcatca
     1201 tcagaaaaaa gaacttgctg cttgccgaaa agaacagggc gctggaagaa gcgaataaaa
     1261 aaatggccga aacagaagtt aacttaaggg agctaaatgc caccaaggac aagctattct
     1321 ctataatcgc tcatgacctg aaaaatccct tcaatgcgct gctgggattt agcgatatcc
     1381 ttgagcgcaa ctacaacttc ctctccgatg aggagcgtat tgagtacatt ggagttataa
     1441 atgattccac ccaatcgctt tataagctgc ttgataacct gctgcaatgg accagaactc
     1501 agaccggaac aataacctac atacccgaaa acttcaagct tcttcctctc gttaagcagg
     1561 agataaccaa cgtcaacctg aactccgaga agaaaaaaat cagcattacc gttaacattg
     1621 acgagaacat tagcgtgttt gccgataaga acacaaccgc tactataatc cgcaacctgc
     1681 ttagcaacgc cattaagttc accgatttgg gtggccacat agagatagcc gcacgctata
     1741 aggatcaaat ggttgaaaca tctgtaagcg attcgggaat tggaataaac gataatgacc
     1801 ttgacaggat tttcatgctt gatggctcat tcacaacaaa aggaacatcc aacgaatcgg
     1861 gtaccggttt gggactactg ctttgtaagg agtttgttga aaaaaacaac ggtaaaatct
     1921 gggtggaaag tgaaaaggga aagggcagca cctttttctt cacccttcca gcaaaataag
     1981 ctcacgcaca cccctcctaa ttttatcata attttttaat cagccaaccc atttctagtc
     2041 cagtataatt ttgtattttt taccgatttt tctgaacaca aaacatgaag ctggttattc
     2101 aaaaatgggg tgtagcccta ctgcttaccg cttacagcct tatagctgct caggcaggtt
     2161 caccaagcag cagtaaggtg gatagcctat accaaagagt gctggcaata gccgacgaaa
     2221 atccacagga agccagcagg cttgccaacc tgtgcaggca ggaatcatct aaatcaggaa
     2281 atagtcgtga taa
//
LOCUS       DAOOQX010000309         2289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_57778_length_2289_cov_2.706356, whole genome shotgun sequence.
ACCESSION   DAOOQX010000309 DAOOQX010000000
VERSION     DAOOQX010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57778_length_2289_cov_2.706356"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..375)
                     /locus_tag="PLX53_05680"
     CDS             complement(52..375)
                     /locus_tag="PLX53_05680"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HOY72608.1"
                     /translation="MERRHFTNFDIAGFGYYDGPIAFSELKVGTELQLVPELNNPYDP
                     KAVIIQYKEYKLGYVPRGENDHISKLLEMGYNPFEVRIQRIDPTAKPEQQIGLVVYLR
                     KADRV"
     gene            complement(426..1325)
                     /locus_tag="PLX53_05685"
     CDS             complement(426..1325)
                     /locus_tag="PLX53_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOY72609.1"
                     /translation="MDQPKLERLLHLIQLLVGNRHNTVELAKRLECSQKTIQRYIDTI
                     QNAGFVVEYHRRGVPFLSTNQGSLKEISDLVHFSPEEAYLLRIAIDSINDDTALKQNL
                     KKKLYNLYNYPWLADIIVKPEQGENVQRLIEAISDERCVILRNYRSSNSNRVDDRLVE
                     PYKFTTNYQQVWCYEHSSQTCKMFKISRIETVEILDRPWECKDKHTDARIDVFRISAE
                     SYVGTVVLRLNLRAYNLLVEEYPLSEQHITADGDNAHLFTCPVCSYEGPGRFVMGLYH
                     DVQILGDDHFISFIRQKVSALHS"
     gene            complement(1411..>2289)
                     /gene="pgeF"
                     /locus_tag="PLX53_05690"
     CDS             complement(1411..>2289)
                     /gene="pgeF"
                     /locus_tag="PLX53_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOY72610.1"
                     /translation="LPAELVRLLADLQMPIFGMFAHGFAQRVMIYIQAQIPYYRFEQM
                     AEHSDTIVQFVSTRNGVRDNRFTIGLNGYAADSQVLANRQMLAAQHGFEPSAFVFAEQ
                     VHGNVVEYISPDKRGRGAFSRSTALPHCDAMITHHPNICLVAQAADCVPILFYDPVKK
                     AIGAAHAGWRGTAAKIAAAVVGAFQERYSSDPRDILVGIGPSIGPCCYEVGDEVAQQV
                     REAFPDGWQAVLQPSPSGKQIFNLWQANRITLLAAGITPQNMEVAEICTRCNNHQFFS
                     ARAGDHGRFGAFIMLR"
BASE COUNT          487 a          574 c          648 g          580 t
ORIGIN      
        1 cattccgttt tcatttccat tatgctttgt tacagtccct tggcggcttt tttacaccct
       61 atctgccttc ctgaggtaaa ccaccagccc gatctgctgc tcgggtttgg cagtggggtc
      121 aatccgctgg atgcgaacct caaatgggtt gtaccccatc tcgagcagct tgctgatgtg
      181 gtcgttctca ccccggggca cgtagcccag cttgtactcc ttgtactgga taataaccgc
      241 tttggggtcg taggggttgt tgagctcggg aaccagctgc agctcggtgc caaccttaag
      301 ctcgctgaat gcaatggggc cgtcgtagta gccaaagccc gcaatgtcga aattggtgaa
      361 gtgtctccgt tccataatat taatttttat gccaagctac acctgcaatt cgacaccttt
      421 tgtcgctagg agtgaagggc tgaaactttt tgacgaatga acgaaatgaa gtggtcatcg
      481 cctaaaatct gcacatcgtg gtacagcccc atcacaaacc gcccgggacc ctcgtagctg
      541 catacagggc aggtgaacag gtgagcgttg tcgccgtcgg cagtgatgtg ctgctccgaa
      601 agggggtact cctcaaccaa caggttgtag gcccgtaggt tgagtcgtag gactaccgtg
      661 ccaacgtagc tttcggccga gattcggaac acgtcaattc tggcatccgt gtgcttatcc
      721 ttgcactccc atggcctatc gagaatttca acggtttcga ttctggaaat cttaaacatt
      781 ttgcaggttt ggctggagtg ctcgtagcac cacacctgct ggtagttggt ggtaaacttg
      841 tagggttcca ccagcctgtc gtcaaccctg ttgctgttcg aggagcggta attcctgaga
      901 atgacgcatc tctcgtcgct gatggcctca atcaggcgct gcacgttttc gccttgttcg
      961 ggcttaacaa tgatgtcggc cagccagggg tagttgtaga ggttgtagag cttctttttc
     1021 aggttctgtt tcagggcggt gtcgtcgtta atgctgtcga tggcgatgcg gagcaggtag
     1081 gcctcctcgg gagagaagtg cacaaggtcg ctgatctcct taaggctgcc ctggttggtt
     1141 gaaagaaatg ggacaccgcg ccggtggtac tccaccacaa aaccggcgtt ttggatggta
     1201 tcaatgtacc gctgaatggt tttctgggag cattcgagcc gtttggccag ctcaactgtg
     1261 ttgtggcggt tgcccaccag cagttgtatc aggtgtagca acctttccag cttgggttgg
     1321 tccatggtgt agggggtgtt ggtttgccat taaaggtagc agctggtttt gtgaaatcca
     1381 aacctttacc acaggtggtt gcttcggcag ctacctgagc atgatgaagg cgccaaaccg
     1441 tccgtggtca ccggcgcgag ccgagaaaaa ctggtggttg ttgcacctgg tgcaaatttc
     1501 ggcgacttcc atgttttgtg gtgtgattcc ggctgccaaa agggtgatgc ggtttgcctg
     1561 ccagaggtta aaaatttgtt ttcccgaggg ggaaggttga aggactgctt gccacccatc
     1621 ggggaatgcc tccctaacct gttgggccac ctcgtcgcca acttcgtagc agcatgggcc
     1681 aatggagggg ccaattccta ccagaatgtc ccggggatcg gagctgtaac gctcctgaaa
     1741 ggcaccaaca accgctgctg caattttagc agctgttccc ctccaaccgg catgggcagc
     1801 accgatagcc ttcttgaccg gatcgtagaa gagaattgga acacagtcgg cagcctgggc
     1861 taccaggcag atgttgggat ggtgggttat catggcgtcg cagtggggta gggcagttga
     1921 acggctaaat gcacctctgc cccttttgtc gggtgagatg tactccacca cgtttccatg
     1981 tacctgctcg gcaaatacga aagccgatgg ctcaaaccca tgctgggcag ccagcatctg
     2041 cctgttagcc agaacctggc tatcggcggc gtaaccgtta agcccaatgg taaagcggtt
     2101 gtcccttaca ccattccggg tagagacaaa ttgcacgatg gtgtcggaat gctccgccat
     2161 ttgttcgaaa cggtagtagg gtatttgagc ctgaatgtag atcatgacac gttgggcaaa
     2221 accatgtgca aacataccaa aaattggcat ttgcaaatcc gccagtaggc ggacaagttc
     2281 cgccggtag
//
LOCUS       DAOOQX010000310         2283 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_58013_length_2283_cov_2.651257, whole genome shotgun sequence.
ACCESSION   DAOOQX010000310 DAOOQX010000000
VERSION     DAOOQX010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58013_length_2283_cov_2.651257"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PLX53_05695"
     CDS             <1..428
                     /locus_tag="PLX53_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOY72611.1"
                     /translation="NHACVMARVKELCPVVQVGIRSMDSSEKTNIDPHRVFWAHQLVA
                     NNSWMDEAIDLITPNVYITFDLDGFDPSIFPSTGTPEPGGLLWYPTLTFLRKVIESRN
                     LVGFDIVELCPNPLDKSSDFLAAKLLYKILSYKYLNTVV"
     gene            461..658
                     /locus_tag="PLX53_05700"
     CDS             461..658
                     /locus_tag="PLX53_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72612.1"
                     /translation="MNKCKCVKVPPPEVGGKEFKLNAYYEFDYIPPVKDNPSFYKVFS
                     LEDNVSGSFSIKAFNEHFKKY"
     gene            complement(847..1482)
                     /locus_tag="PLX53_05705"
     CDS             complement(847..1482)
                     /locus_tag="PLX53_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOY72613.1"
                     /translation="MGVSPVEIENMISQTPHVVKNFSKGQLVAQRNDEVKSLTILVEG
                     SVKGEMADFSGKILKIEEMQAPKPIAHAFIFGEHNQYPVDVVALDDCRILYIPKADFL
                     ALMQRNATVLINYLNSISNRTQFLSNRLWFLSFKTIKEKIAHYLLNLSYGGANATLIL
                     PKSQQELAEFFGVTRPALARVFSDLEVEGIISIKRREVVIRDRNKLIEIAS"
     gene            1695..>2283
                     /locus_tag="PLX53_05710"
     CDS             1695..>2283
                     /locus_tag="PLX53_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72614.1"
                     /translation="MNDHQTGKLDISFNTDLPEPLVFSTWKHHLNYIHDAILSIEVGS
                     ETTSLINEVAAGVGGTLVDLYIGELTPMVIAEEVIQQLVSFDALGYDSFSRWLGVSKS
                     GYQNMYLSDGSAWILRLGMVNRRYVHIHPARYSLNTVRCRSANLKFAIAYRLLYGMDD
                     SNFSIDKVNRTRETLGLPPAAEKIALSGALRILKQL"
BASE COUNT          632 a          465 c          511 g          675 t
ORIGIN      
        1 ataaccatgc atgtgtaatg gccagggtta aggagctgtg tcctgtggtt caggttggca
       61 ttcgtagcat ggatagctcc gaaaaaacca acatagatcc tcatcgtgtt ttctgggctc
      121 atcagcttgt cgccaacaat agctggatgg atgaagccat cgatcttata accccaaatg
      181 tttacattac atttgatttg gatggatttg atccttccat ttttcctagc accggaacac
      241 cagaaccggg aggtttgctc tggtacccca ctttgacatt tttaagaaaa gtgattgaat
      301 caagaaacct tgtaggattc gatattgttg agctttgtcc aaaccccctt gataaatctt
      361 cagactttct tgcagccaag ttgctatata aaatactgtc ctacaagtac ttgaatacag
      421 tcgtctgaat tttagaaagt ataaccaacc tgaattagat atgaataagt gcaagtgtgt
      481 taaagttcca cctcctgagg tcgggggcaa ggaattcaag ctgaacgctt actatgaatt
      541 tgactacatc cccccggtta aggataatcc atcgttttat aaagttttta gccttgagga
      601 taacgttagt ggcagtttta gcattaaggc atttaacgag cactttaaaa agtactgaaa
      661 gagccagtaa atccccaaag caatgcttgg ggtaggggtt tggtgctagt gctaaaacta
      721 gccagagtcg gcctttagtt attcaggtag tctatttcgt tgcagaatac tagacatcta
      781 aatggcttgt ccatttttaa cagggctggc cgggtgtaaa tagttgaatt tcaagtcttc
      841 caaaagttag ctggcaattt ctatcagctt gttcctatcc ctaataacca cctcgcgtcg
      901 ttttatgctt ataatacctt cgacctccaa atcggagaaa acacgggcaa gggctggtcg
      961 ggttacccca aaaaattctg ccagctcctg ttgtgatttg gggagtatca gggtggcgtt
     1021 agccccaccg taggaaagat ttagcaagta atgtgcaatt ttttctttaa tggttttgaa
     1081 ggacaggaac caaagcctat tggagagaaa ttgagttctg tttgatatgg aattcaagta
     1141 gtttataagg actgtggcgt tccgctgcat gagcgctagg aaatcggctt taggaatgta
     1201 gagtatgcgg caatcgtcga gcgcaaccac atcaacagga tactggttat gttcgccaaa
     1261 aataaaggcg tgtgcaatgg gctttggtgc ctgcatctcc tcaattttca gtattttacc
     1321 cgaaaaatcg gccatttcac ccttgacact tccctcaacc aatatggtaa ggcttttcac
     1381 ctcatcgttc cgctgggcta ccagctgccc tttactaaaa tttttcacca catgtggggt
     1441 ttggcttatc atgttttcaa tctctacggg gctaaccccc atgaaaagtg gtgattttga
     1501 caaaatcggg tacatatacg agtaggttta gtaaattttt gcatgaatat agtggtaatt
     1561 acgtaaatta acttcaaaaa aacattcctc gtatttgggg cgaaattcgt cgctcgggtt
     1621 aaatggtcta tagaagatct tatgtttgtt atatttttga aggaatgccc tataattgtg
     1681 ataattgcta tgccatgaac gaccaccaaa ctggcaagtt agacatttcg tttaataccg
     1741 acctgccgga accattagtt ttcagcacat ggaagcacca cttgaactat atccacgatg
     1801 ccattttaag cattgaggta ggcagcgaaa caacttctct catcaacgaa gttgcagcag
     1861 gcgttggagg tacactggtt gatttataca tcggagagtt aaccccaatg gttattgccg
     1921 aagaggttat ccagcagctt gtaagctttg acgcattggg ctatgatagc ttttcccgat
     1981 ggcttggtgt ttccaagagc gggtaccaga acatgtacct gagcgacggg tcagcatgga
     2041 ttttaagatt gggtatggtt aaccggcgtt acgttcatat tcatcctgcc agatactcgt
     2101 tgaataccgt gcggtgccgg tcggccaacc tgaaatttgc tattgcatac cggttgttat
     2161 acggtatgga cgattcaaat ttttcaatcg acaaggtaaa taggactaga gaaaccttgg
     2221 gattaccacc tgcagctgaa aaaattgccc tctcgggggc attaagaatc cttaagcagc
     2281 taa
//
LOCUS       DAOOQX010000311         2279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_58174_length_2279_cov_2.415018, whole genome shotgun sequence.
ACCESSION   DAOOQX010000311 DAOOQX010000000
VERSION     DAOOQX010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58174_length_2279_cov_2.415018"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PLX53_05715"
     CDS             complement(<1..200)
                     /locus_tag="PLX53_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72615.1"
                     /translation="MVTGSGRLDSATEVEPVDIRLFRCYIYDDIKEWERVIGVLENTY
                     RMKPTIDDEFQLLHAQYGFIPYL"
     gene            complement(293..1375)
                     /locus_tag="PLX53_05720"
     CDS             complement(293..1375)
                     /locus_tag="PLX53_05720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOY72616.1"
                     /translation="MVIFYLVLLLVVFAVLRLVVVLVNYIYTPYLRPFELIDHGKVLV
                     VVGQGKNTASLVNLLHDLQNQSYTNFEVLVYCSSPLNSIPDDQLAFARVDNRFKFLNG
                     TIREAGESYSSNFRNVLVEKSSGSYILFLDPDMRMGIHFINAMVANIKHRKLAVLSVT
                     PHYDTTKSFGVKIAVSAVQWLMLSLIPLKLIGKHFNPKLPLTDGMPVMYNAEVFRRHT
                     LTSVTIPEDLKSAMYSKEFKQLRLRSFTLLGRKEEISCSYFTSYRYALRRVAGGLFDF
                     FSGREWLMFSYAILTTLGPFITLLAMPYPFTFAYLFSVLLGRMLVAALCEQSAWSAIL
                     LFPLQQLVLFRMMLSPLHSSITGHKR"
     gene            complement(1699..2211)
                     /locus_tag="PLX53_05725"
     CDS             complement(1699..2211)
                     /locus_tag="PLX53_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOY72617.1"
                     /translation="MGKEAKQVVSVDLEKLLGMLNAALAEEWLAYYQYWIGARVMEGP
                     MRSEIEPELLLHATQELNHAVLVVGRIIQLGGTPILSPQDWFKVNRCTYDAPVDPYIE
                     VVLEQNLKGERCAIKRYQEIADFTNGKDHSTHQMAVQILNEEIEHENDIEDWITDINR
                     MKEEFKKMRM"
BASE COUNT          705 a          502 c          448 g          624 t
ORIGIN      
        1 aggtagggaa taaagccata ctgggcatga agaagctgaa attcatcatc aatggttggt
       61 ttcattcgat aggtgttctc caaaacccct attacccttt cccattcttt tatatcatcg
      121 taaatgtagc accggaagag gcgaatatcc acaggttcga cttcggtagc tgaatctaac
      181 ctcccgctac ctgtgaccat taacagaaaa gtaagcgcac ctccaaacaa gaaataacca
      241 aaatgctttg ttctagccat aaacgttaat aatacactaa ttagcttaat ttctatcttt
      301 tatgtcctgt aatgctgcta tgaaggggtg aaagcatcat tctaaacaaa actagctgct
      361 gaaggggaaa aagtagaata gccgaccatg ctgattgttc gcatagagct gctacgagca
      421 ttcggcctaa tagcaccgaa aaaagatatg cgaaagtaaa gggataaggc atagctaaaa
      481 gagttataaa aggcccaagg gtggtaagaa tggcatagct gaacattagc cactctctgc
      541 cggaaaaaaa atcgaacaac ccacctgcaa cccttcgcaa cgcataacgg tagcttgtaa
      601 aatagctgca ggaaatttcc tcctttcttc ccaacagcgt aaacgatcgg agtcgtaact
      661 gcttaaactc cttgctgtac atggcgcttt taaggtcttc gggaatggtc acagaggtta
      721 gagtatgcct tctaaagact tcagcgttgt acatcaccgg cattccatct gtcagtggca
      781 acttagggtt aaaatgtttt ccaatcagct ttaaaggtat aagtgatagc atcaaccact
      841 gaacagccga aacagcaatt ttcacgccaa aacttttggt tgtatcataa tgtggtgtaa
      901 cggaaagcac ggctagcttt ctgtgcttaa tgttggcaac cattgcgtta ataaaatgaa
      961 tacccatccg catgtcagga tcaaggaaaa gtatgtagct gcctgatgat ttttcgacaa
     1021 gcacattgcg aaaattactc gaataggatt caccagcttc acgaatcgtt ccatttaaaa
     1081 acttaaaccg gttatcaacc ctggcaaaag ccaattggtc atcgggaata ctgttcagag
     1141 gactgctaca ataaaccaac acctcgaagt ttgtgtagga ctggttttgc aagtcgtgca
     1201 ggaggttaac aagactagct gtatttttgc cctggccaac cacaaccaat acctttccat
     1261 ggtctatcag ctcaaaaggt cttaggtagg gggtataaat gtagttaacc aaaacaacca
     1321 ccaacctaag cactgcaaaa acaaccagca acaaaaccaa gtaaaaaatt accattctct
     1381 atataactat cattataaca aagcctaccc tatacaactt taatttacaa aggtaaaact
     1441 tttcaatata gtgagaattg caaaggtaag gcctgctcaa atacaatacc ccaaagcatc
     1501 attgattgtt agcttaaaga tggagtgtta ttgtgcaata ataggcttta tagttatagg
     1561 ctacattata ggcaattctt tttcgacata ccacgctcca atcatcatta aaaaaaacga
     1621 ttcctataag ggaaccgttt actaatacac acggggctat gttggcatag tctcagcata
     1681 caagcaatga aattgacatt acatacgcat ttttttgaac tcctccttca tgcggttgat
     1741 atcggtaatc caatcctcaa tgtcgttttc gtgctcaatt tcctcgttaa gaatttgaac
     1801 ggccatttgg tgggttgagt ggtctttccc attggtgaaa tcagcaattt cctggtaacg
     1861 tttaattgcg caacgctcgc ccttaaggtt ttgctccaga accacctcaa tgtaggggtc
     1921 aacgggggca tcgtaagtgc aacggttaac cttaaaccag tcctgtgggc ttagtatagg
     1981 cgttccgccc agctgaatta ttcttccaac aacaagtaca gcgtggttta gctcttgtgt
     2041 ggcatgaagc aggagctcag gttcaatttc gctccgcatg gggccctcca tcactcgggc
     2101 accaatccag tactggtagt atgcaagcca ctcctcggct agtgcggcat taagcattcc
     2161 cagcagtttt tctaaatcaa ccgatacaac ttgtttggct tcttttccca tagtaacaat
     2221 gttttttaaa gagtattcgc tactttatac gacggacata aagagtttgt ttgccaaat
//
LOCUS       DAOOQX010000312         2277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_58218_length_2277_cov_3.621062, whole genome shotgun sequence.
ACCESSION   DAOOQX010000312 DAOOQX010000000
VERSION     DAOOQX010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58218_length_2277_cov_3.621062"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1128)
                     /gene="amrS"
                     /locus_tag="PLX53_05730"
     CDS             complement(124..1128)
                     /gene="amrS"
                     /locus_tag="PLX53_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOY72618.1"
                     /translation="MEAKYYSKKGNGIRCNLCPHSCSIQDGRFGICKARQNQGGVLNA
                     LGYGVISSSGLDPMEKKPLYHFHPGSRIFSIGSYGCNLHCDFCQNFEISQSFPVSMPP
                     LRSYQPSEVVEKARVLPENIGIAYTYNEPTISFEFMLETARLAKSKGLLNVAVSNGYI
                     NPAPLRELLEVLDAFNIDLKSFSDDFYCSQTGGRLKPVLKTLVTIRELGKHLEIAFLV
                     IPGLNDSLAETKAMVSWIASNLGSNTVLHINRYYPAYKLKIPPTPEERLVELYEIARE
                     KLAYVYLGNLHGNEVGCNTICPSCSSTVIRRNGYHTVISALTREGRCSSCGYGPIVVM
                     "
     gene            1589..>2277
                     /locus_tag="PLX53_05735"
     CDS             1589..>2277
                     /locus_tag="PLX53_05735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY72619.1"
                     /translation="MEKKLRRRVLVITYYWPPSGGVGVQRWLKFVKYLRLYGWEPVVY
                     TPENPESFYDDESLLNDIPEGVEVIKTRIWEPYKIYKRITGTKNEKIAVGFTSSSNKK
                     KGLLHRVAVWLRGNLLIPDPRVFWVNPSVKFLKKYLRESPVDVVVTTGPPHSIHLIGL
                     KLKQKMKVKWLADFRDPWTNIYYIKDLRLSAPAMRIHRRLESKVVKEANRVVVVSEQM
                     KREFQQLAAKR"
BASE COUNT          693 a          500 c          478 g          606 t
ORIGIN      
        1 aaacgtgacc ttagcgagct gatttggaac aagacagtcg gtaaaggtta aacaattaca
       61 ttcttctaat cgccaaagcc aggtaatcca acgctattct tcaacaggaa tagcttagca
      121 gccttacatt acaacaattg gtccgtagcc acaactgctg cacctaccct cacgggttag
      181 ggcggagata acggtgtgat agccattcct acggataacc gtggaggaac acgacggaca
      241 aatggtgttg cagcccacct cgtttccatg caggttgccc aagtacacat aggctagctt
      301 ctctcgagct atttcgtata gctcaaccaa cctttcttcg ggagtaggcg gaattttcag
      361 cttgtaggca gggtagtagc ggttgatatg caaaaccgtg ttgcttccca gattcgaagc
      421 aatccacgaa accattgcct tggtttcggc aagcgaatcg tttagtcccg gaatgaccag
      481 aaaggcaatc tccaaatgtt tacccaattc ccttatggtc accagagttt tcagcacggg
      541 cttgagcctt cctcctgttt gactgcagta aaaatcatcg gaaaatgatt ttaaatcgat
      601 attgaaagca tccagaactt caagaagctc cctcaatggt gcagggttga tatacccatt
      661 tgaaaccgca acattcagca aacctttact tttagccaac cttgccgttt caagcataaa
      721 ctcaaatgat attgttggtt cgttgtaggt atatgcaatc ccaatgtttt ccggaagaac
      781 cctggccttc tcaacaactt ctgaaggctg ataactcctc aacgggggca tcgaaacagg
      841 gaaagattgc gaaatctcga agttctggca aaaatcgcag tgcaggttgc acccgtagct
      901 gccgatagaa aatatccggc tacccggatg aaagtggtaa agcggctttt tttccattgg
      961 gtctagcccc gatgacgaaa ttaccccgta tcctagagcg ttgagaaccc caccctggtt
     1021 ttgacgtgcc ttgcatatac caaaccttcc atcctgtatg ctacaactat gagggcaaag
     1081 gttacacctt atgccgttac ccttttttga atagtacttt gcctccatga tcaaaaagca
     1141 catttagaat tatcatcaaa gttcgataaa cttttgcttt aattgcaagt catcccaact
     1201 taattccaac gataccatac ctataggtta aaccaaaatt taaaaaatcg agtgttgaat
     1261 gctttttcac ttcttcaacg cagaaatcat cccacaaaaa tcattaaaaa tagtacctgt
     1321 atgttttaca cctgttggat atcagaaatt ttgaagttta cctgcttcct gcatagcttg
     1381 accaacacaa attaaattta ggatttgcat gataatgttg acgattaaat aactcccgta
     1441 tttacatgta ggtcatagta gtaaaggact gcatgaagcc gctgtaaagt aaaaacatta
     1501 aagagattaa tctgaaatta gggaacttcc actactattc ctttccgggt tgcggccaaa
     1561 tttttacctt tgaactttta tttaataaat ggagaaaaaa ttacgcaggc gtgttctggt
     1621 tatcacctac tactggcccc catcaggtgg tgtaggcgtt caacgttggc taaagtttgt
     1681 aaagtacctc aggctgtacg gatgggaacc ggtggtttac acacccgaaa atcccgaatc
     1741 gttttacgac gatgaatcgc tgctgaatga cattcctgaa ggggttgagg ttataaaaac
     1801 cagaatatgg gagccctaca aaatatacaa aagaatcacc ggcacaaaga acgaaaaaat
     1861 tgccgtgggt tttacctctt cttcaaataa aaaaaaggga ttactgcata gggtggctgt
     1921 ctggctaagg ggcaacctgc ttattcctga tccaagagtc ttttgggtta acccatcggt
     1981 taagttctta aaaaaatacc taagggaaag tccggtggat gtagtggtta caaccggccc
     2041 accgcactcc atccatctaa tcgggctgaa gctgaaacaa aagatgaaag taaaatggct
     2101 agccgatttt agggatccat ggacaaatat atactacatt aaggatttaa gactaagcgc
     2161 tcctgcaatg cgaatccatc gcaggttgga aagcaaggtg gtaaaggaag caaatcgggt
     2221 ggttgtggtg tcggaacaaa tgaaaaggga attccaacag cttgctgcca aaaggat
//
LOCUS       DAOOQX010000313         2271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_58437_length_2271_cov_3.320848, whole genome shotgun sequence.
ACCESSION   DAOOQX010000313 DAOOQX010000000
VERSION     DAOOQX010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58437_length_2271_cov_3.320848"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1161
                     /gene="queG"
                     /locus_tag="PLX53_05740"
     CDS             229..1161
                     /gene="queG"
                     /locus_tag="PLX53_05740"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOY72620.1"
                     /translation="MAMIPLEIVRKLALDVGFSDCGVSPAGKLGDAGNGLLSWLGKGY
                     HGEMAYMERNLNLRLNPSALVPGAKSVISVILAYGHQEIPLMAHPPRVSRYALMPDYH
                     KVMKTMLFRLLNALREEFGPVNGRPFVDSAPVLERAWAENSGMGWIGKNSNFIHPKFG
                     SFVFIGELIVDAAVEDSSRQHKNRCGSCTRCMEACPTHAIVEPGVVDARLCISYLTIE
                     KRSPLTEKECGSLNGWCFGCDICQEVCPWNQKLLPITSEEVSIIPELKQLNAGNMMGM
                     TQTQFEEWFGITPLKRAGYEKLMQSVSCAVKASH"
     gene            complement(1197..>2271)
                     /locus_tag="PLX53_05745"
     CDS             complement(1197..>2271)
                     /locus_tag="PLX53_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOY72621.1"
                     /translation="WCTAQALSFPNGKGPNMIVDDGGDATLLVHWGYKAENDRKFVER
                     KAQNHEEEVILNLIRETLQQEPNKWHNLAAELKGVSEETTTGVHRLYQMKEKNELLIP
                     AINVNDSVTKSKFDNLYGCRESLADGIKRATDVMIAGKVVVVCGYGDVGKGCARSMRS
                     YGARVIVTEIDPICALQAAMEGFEVKTVDDALAEGNIFVTATGNCDIITLEHMQQMKD
                     QTIVCNIGHFDNEIQMDRLNTQKGVRKVNIKPQVDQYYFPDGHSIFILAEGRLVNLGC
                     ATGHPSFVMSNSFTNQTLAQVELWTKHLAVDVYRLPKQLDEEVARLHLEQLGVKLTTL
                     TPKQADYIGVNPLGPYKSDHYRY"
BASE COUNT          552 a          555 c          562 g          602 t
ORIGIN      
        1 ccagccgaac tactcactgt aacacctgtc gaagggtgaa tccgtcagga gccggacaag
       61 ttccgccagt aggcggacag gttcttgaat ccgtcaggag ccggacaagt tccgccagta
      121 ggcggacagg tttttgaatc ttgaatttag gcctttatcc tttatcctta accttattat
      181 catccttatg ctatctttga acccattata tcggtcaaag gagttttgat ggcgatgata
      241 cctttggaaa tagtcaggaa actggcttta gatgtgggtt ttagcgattg cggcgtatct
      301 cctgccggaa agcttggcga tgccggcaat gggttgctaa gctggctggg aaaggggtat
      361 catggcgaaa tggcctacat ggagcgaaac ctcaaccttc gtcttaaccc ttcggcattg
      421 gtaccggggg caaaatcggt aatcagcgtg attcttgcct atggtcatca ggaaataccc
      481 cttatggccc acccaccaag agtttcccgc tatgcactta tgcccgatta ccataaggta
      541 atgaaaacca tgctatttag acttctcaat gccttaaggg aggagtttgg cccagtaaac
      601 ggaaggccct ttgttgactc tgcaccagtg ctcgagcgtg catgggctga aaactctggc
      661 atgggttgga taggcaagaa ctccaacttc atacatccca aatttggctc gttcgtgttt
      721 attggtgagc tcattgttga tgcagcagtt gaagattcca gccgtcagca taaaaatcga
      781 tgcggcagct gcacccggtg catggaggca tgtcccacac acgccattgt agagcctggc
      841 gttgtagatg cccgactttg catttcgtac cttaccattg aaaagaggag cccccttaca
      901 gaaaaggagt gtggaagcct gaacggctgg tgttttggct gcgatatttg tcaggaggtt
      961 tgcccctgga accaaaaact actccctata acgagcgaag aagtctcgat tatcccagag
     1021 ttgaaacagc ttaacgcagg caacatgatg ggcatgacac aaacacagtt tgaagagtgg
     1081 tttggcatta ccccgttaaa aagggctgga tacgaaaagt taatgcagtc ggttagctgt
     1141 gcggtgaaag cttcacatta aaaatgccgg cagctgccgg catttcggta gctatcctag
     1201 taacggtagt gatcggattt gtatgggccg agaggattta caccaatgta atcggcctgt
     1261 ttgggcgtaa gcgttgtgag cttaaccccg agctgctcca ggtgtaacct ggcaacctcc
     1321 tcgtccagct gcttgggaag gcggtaaaca tcaacagcta aatgctttgt ccatagttca
     1381 acttgtgcca aagtctggtt ggtaaagctg ttgctcatca caaagctggg atgacctgtt
     1441 gcgcagccca ggttaaccag tcgaccctcg gcaagtatga atatggaatg accatcaggg
     1501 aagtagtact ggtcaacctg aggctttatg ttgacttttc taacaccctt ttgggtattt
     1561 aacctatcca tctgaatttc gttatcgaag tggccaatgt tgcacacaat agtctgatct
     1621 ttcatttgct gcatgtgctc aagggtaata atatcgcagt tgcccgtagc agtaacaaag
     1681 atgttaccct cggctagtgc atcgtcaact gttttcacct caaatccctc cattgcggct
     1741 tgcagggcgc atattggatc tatttcggta acaattaccc tggcgccata ggagcgcatg
     1801 ctgcgggcgc atcccttacc cacatcaccg taaccgcaaa ccacaactac ctttccggca
     1861 atcatcacgt cggtagctcg tttaatgccg tcggcaagcg actcgcggca gccgtagagg
     1921 ttgtcgaact tgctcttggt aacagaatcg ttcacgttga tggcgggtat cagcagctcg
     1981 tttttctcct tcatctggta aagccggtga actcccgttg tggtttcctc ggaaactcct
     2041 tttagctccg cggcaaggtt gtgccatttg tttggttcct gttgaagggt ttccctaatc
     2101 aggttaagaa taacctcctc ctcatgattt tgggctttac gctccacgaa ttttctatcg
     2161 ttctcggcct tgtagcccca gtgcaccaaa agcgtagcat cgccaccatc atcaacaatc
     2221 atgttaggac ccttaccgtt agggaaagat agagcctgag cagtgcacca c
//
LOCUS       DAOOQX010000314         2270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_58469_length_2270_cov_3.659594, whole genome shotgun sequence.
ACCESSION   DAOOQX010000314 DAOOQX010000000
VERSION     DAOOQX010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58469_length_2270_cov_3.659594"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1191
                     /locus_tag="PLX53_05750"
     CDS             184..1191
                     /locus_tag="PLX53_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOY72622.1"
                     /translation="MVGERIITVEGVDPLAIYGVNNTRFEMISRHYPKLKLVARGNQI
                     KVTGDEELIDDLESKINRMVEFYSRYSRLTEANILDFLNDNDSSIVKAEEDALDVLVY
                     GTNGRTVKARTINQRKLVELYQTNDLLFAIGPAGSGKTYTAIALAVRALRNKEVKRIV
                     LTRPAVEAGERLGFLPGDMKDKLDPYLQPLYDALHDMIPQKKLMEYMDDGTVQIAPLA
                     YMRGRTLDNAFVILDEAQNTTLNQLKMFLTRMGKNAKFIVTGDITQIDLPNRGDSGLV
                     KAIGILENIGSIAMVYFDARDIVRHKLVKHIVNAFERYTSGNGSSERTENNDECEDNS
                     Q"
     gene            1363..>2270
                     /locus_tag="PLX53_05755"
     CDS             1363..>2270
                     /locus_tag="PLX53_05755"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOY72623.1"
                     /translation="MEAIVRTDFKFPGQTSVYHGKVRDVYNIANKHLIMVATDRISAF
                     DVILPKGIPYKGQVLNQIAAKFLDATADIVPNWKIATPDPMVTVGRLCEPFKVEMVVR
                     GYLTGHAWREYKSGNRLLCGVPLPEGMREHQKLPAPIITPTTKAIEGHDEDISREEII
                     ASGLVEEHDYAQLERYTLALFERGTRMAEKMGLILVDTKYEFGRADGRIYLIDEIHTP
                     DSSRYFYADGYTERFNRGEEQRQLSKEFVREWLMANGFQGQEGQKVPEMTPEFVNQVS
                     ERYIELYEQITGEKFQRAEGSDVMKR"
BASE COUNT          635 a          503 c          566 g          566 t
ORIGIN      
        1 tggctgtata aaaatatttg cgccaggggc accccattta acaggaatgg gttatatttg
       61 tgaggttttg gttattaaca atagcttgtt tactaaattc aatctggttg agaactcaaa
      121 tagccttaca atgttttgat tatattgccc agctcccgtt gggtggcaat cttgtgaaat
      181 tttatggtag gagagcggat tataactgtt gagggagttg atcccctggc catttacggg
      241 gtgaacaaca cccgtttcga gatgatttcc cgccactacc ccaagttaaa attggttgcc
      301 aggggtaacc agattaaggt aaccggcgac gaggagctga ttgatgacct ggagagcaaa
      361 ataaaccgca tggttgagtt ttacagccga tactcccgat taacggaggc taacatactt
      421 gattttctca atgataacga cagctcaatt gttaaggccg aggaggatgc tctggatgtg
      481 ctggtttatg gtacaaacgg ccgaactgtt aaggcgcgaa ccattaacca gcgaaagctg
      541 gtggagttat accaaaccaa cgacctgctt tttgccatag gacctgctgg ttcgggcaaa
      601 acctatacag caatagctct ggctgtaagg gcactccgaa acaaggaggt gaagcgaata
      661 gtgcttaccc gtcctgccgt ggaggccggt gaaaggttgg gctttcttcc cggggatatg
      721 aaggataagc tcgaccccta ccttcaacct ctttacgatg ccctacacga catgatacca
      781 cagaaaaaac tgatggagta tatggacgat gggactgttc agattgctcc cttggcctac
      841 atgcgcggga gaaccctcga caatgccttc gttatactcg acgaggccca gaacaccacc
      901 cttaaccagc tcaaaatgtt tcttacccga atgggtaaaa atgccaagtt cattgttacc
      961 ggtgacatca cccagattga ccttcccaac agaggtgatt ctggattggt aaaggcaatt
     1021 ggaattttgg aaaacattgg tagcattgcc atggtttact ttgacgcaag ggatattgtt
     1081 cggcataagc tggtgaagca cattgtgaat gcctttgaga ggtacacctc cggtaatggg
     1141 agttctgaaa ggactgaaaa taatgatgag tgtgaggata actctcagta gtaaccttgg
     1201 ttctagtgaa gtgccatttg caacagcatt atcttctaaa cactaaacat tgtgggcgtt
     1261 ggggtgtttg ccaccaagaa actttttggt aatcctaata cactgcactt taacctcaat
     1321 ggaacagaag ccaaatcaac tcaaactaaa atacagaagc aaatggaagc gattgtaaga
     1381 acagatttca aattccctgg ccaaacctct gtgtaccatg gaaaagttcg tgatgtttac
     1441 aatattgcca acaagcacct gattatggtg gcaaccgata ggatatcggc cttcgacgtt
     1501 atccttccca aaggcattcc ctacaagggg caagtgctta accaaatagc tgctaagttt
     1561 ctggatgcca cagccgacat agtgcccaac tggaaaatag ccacccccga tccgatggta
     1621 accgttgggc gcctgtgtga accatttaag gtggagatgg tggtgagggg ctacctcacc
     1681 ggtcatgcct ggcgggagta taaatcgggt aaccggttac tttgtggggt gccacttccc
     1741 gaagggatgc gcgagcacca gaaacttccc gctcccataa tcacaccaac taccaaggcc
     1801 attgaaggtc acgatgagga tatttcacgt gaggaaatta tcgccagcgg gctggttgaa
     1861 gagcatgact acgcacaact tgagcgctat acactcgctc tttttgaacg cggcacccga
     1921 atggccgaaa agatggggct aattcttgtt gacaccaagt atgagtttgg acgtgccgac
     1981 ggccgcatct acctgattga tgaaattcat acacccgatt cttcccgtta tttttacgcc
     2041 gatggctata ccgaaaggtt caaccggggt gaggagcaac gtcagctttc taaagaattt
     2101 gttcgcgaat ggcttatggc caacggtttt cagggacagg agggacaaaa ggttccggaa
     2161 atgaccccag aatttgtaaa ccaggtttcg gagcgctaca tagagctcta cgagcaaata
     2221 accggcgaga aatttcagag ggcagagggt tctgatgtaa tgaagcgtat
//
LOCUS       DAOOQX010000315         2269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_58516_length_2269_cov_2.478320, whole genome shotgun sequence.
ACCESSION   DAOOQX010000315 DAOOQX010000000
VERSION     DAOOQX010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58516_length_2269_cov_2.478320"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLX53_05760"
     CDS             complement(<1..741)
                     /locus_tag="PLX53_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOY72624.1"
                     /translation="MAKYKMHLLVCGGTGCRASSSDLIVDRLKAEIASNGLENDVQVI
                     MTGCFGFCEKGPIVKVLPDNTFYTQVKPEDAAEIVREHALKGRKVTRLLYTDPVKKEH
                     VSDSKHMGFYRKQIRIALRNCGFINPENIDEYIARDGYQALGKVLTEMDPQKTIDIIK
                     KSGLRGRGGGGFPTGLKWEIASKNKADQKYVVCNADEGDPGAFMDRSVLEGDPHSVLE
                     AMAICGYSIGASKGLIYIRAEYPLAIKRL"
     gene            complement(763..1164)
                     /locus_tag="PLX53_05765"
     CDS             complement(763..1164)
                     /locus_tag="PLX53_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOY72625.1"
                     /translation="MTKVKSLADLKMMKDSLKSKISLREKGENSENLVQIKVAMATCG
                     IASGAKVVMDFLLEELDKRGINSVVTQTGCMGYCYAEPTIEVKRPGQEPVVFGYVDTK
                     KADEIIEKYIKNGELVDGIIPTNYETIDGEK"
     gene            complement(1279..1827)
                     /locus_tag="PLX53_05770"
     CDS             complement(1279..1827)
                     /locus_tag="PLX53_05770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY72626.1"
                     /translation="MRDLSLHLLDIVQNSIAAQATKIDVHLEINPKLDYLALIISDNG
                     KGMNAEFLQQITSPFTTTRTTRKVGLGLPLLMQNAERTGGYLEITSSVGKGTTLRAVF
                     HPNHIDCPPMGDIAGVAAQLICSFPNISFSFSFKTDTSEFSITTLEIAQALEGIPIND
                     TSVFPLVKQVIESSLEELKFPV"
     gene            complement(1873..>2269)
                     /locus_tag="PLX53_05775"
     CDS             complement(1873..>2269)
                     /locus_tag="PLX53_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HOY72627.1"
                     /translation="NEKEEIIEEVEYSLHGALNVGINELEAVVHAMNGIFIPAHIDRP
                     KYSLTSQLGFVPPDLNYDALEVSINSSIESICVHNPSLKRKRFIRSSDAHFIEQVGSV
                     TTTFRMENLNFDGLLKAIKGIDGYDIMLP"
BASE COUNT          611 a          536 c          454 g          668 t
ORIGIN      
        1 tagccgttta atagccaatg ggtactccgc ccgaatgtaa atgagccctt ttgaagcacc
       61 aatactataa ccgcaaattg ccattgcttc tagtactgag tgtgggtcgc cctctaggac
      121 agaacgatcc ataaaagcac ctgggtcgcc ctcgtcggcg ttgcaaacca cgtacttctg
      181 atcggctttg ttcttgctgg caatttccca ctttaaaccg gtaggaaagc cgccaccacc
      241 acgtcccctt aatcccgatt tcttaatgat atcaattgtt ttctgcgggt ccatttcggt
      301 aagcactttt ccaagcgcct ggtagccatc acgggcaatg tactcgtcaa tgttttcggg
      361 attaataaaa ccacagtttc ttagggcaat tctaatttgc ttgcggtaga aacccatgtg
      421 ttttgagtcg gatacatgtt ccttttttac cggatcggtg tagagcaggc gggtaacctt
      481 ccttcccttc agggcgtgct ccctaacaat ttcggcagca tcttcaggtt taacctgggt
      541 gtaaaaggtg ttatcaggaa gcaccttgac aatagggcct ttctcacaaa acccgaagca
      601 accagtcatt ataacctgaa catcattctc caggccgttg gaagcaatct cagctttaag
      661 cctgtcaaca atcaaatcgc tcgacgaagc cctgcagccc gttccaccac aaaccagcag
      721 gtgcatttta tactttgcca ttacttctgt cttttaagag ggttattttt caccatcaat
      781 ggtttcgtag ttggtgggga tgatgccatc caccaactcg ccatttttga tgtacttttc
      841 aattatttca tcagcctttt tagtatcaac gtacccaaaa accacgggtt cttggccggg
      901 acgcttaacc tctatggtgg gttcagcata gcagtaaccc atgcatccag tttgggtcac
      961 tacggagttt atcccccgct tatccagctc ctcgagcaaa aagtccataa ccacttttgc
     1021 tccagaagca atgccacatg tggccattgc caccttaatc tgaacgagat tttcagaatt
     1081 ttcccccttt tcccgtagtg agattttcga cttcagggag tccttcatca ttttcagatc
     1141 agccaatgat tttacttttg tcatagcaat actccttaaa cattcatttt tattgggtat
     1201 atatcgtttt gaacttttcg gtggcaaaat tacattttaa atgttaacaa actaacaaca
     1261 aattatatgt ttaataacct aaacgggaaa tttgagctcc tccagggagg attctatcac
     1321 ctgcttaact agcggaaaaa cactggtatc attaatagga atgccctcca gtgcttgagc
     1381 tatttcaagt gtggtaatgc taaactctga agtatctgtt ttaaaactga agctaaacga
     1441 aatgttaggg aacgaacaaa taagctgagc agcaacaccc gctatatcgc ccataggagg
     1501 gcaatctatg tggttaggat gaaaaacagc tctaagcgtt gttcccttac caacagatga
     1561 ggtaatttcc aggtaaccac ccgtgcgctc agcattttgc attaaaaggg gtaatcctaa
     1621 tccaaccttg cgggttgtac gggttgttgt aaaaggactc gttatctgtt gaagaaactc
     1681 ggcattcata ccttttccat tatcggatat gataagtgca aggtaatcaa gttttgggtt
     1741 aatttcaaga tgaacatcaa ttttggttgc ctgggcagca attgaatttt gaactatgtc
     1801 gagcaggtgc aacgataaat ccctcatatc aaaatcatct aaagtatagg cagcctcatc
     1861 aggctagctc cctcagggga gcataatatc gtagccgtca atccctttaa ttgccttcaa
     1921 caagccatcg aagttcaggt tttccatcct gaaggtagtg gttacagacc caacttgttc
     1981 tatgaagtga gcatcggaac tcctaataaa tctttttcgt ttaagcgatg ggttgtgtac
     2041 gcagatactt tcaattgaag aatttatgga tacctccagc gcatcatagt ttaggtcggg
     2101 gggtacaaaa cccagctggc tggtcaagct atacttcggc ctgtcaatat gagccggtat
     2161 gaaaattcca ttcattgcat gcacaacagc ctcaagttcg ttgatgccaa catttaacgc
     2221 cccatggagt gagtactcaa cttcctcaat aatttcctcc ttctcgtta
//
LOCUS       DAOOQX010000316         2266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_58625_length_2266_cov_2.868385, whole genome shotgun sequence.
ACCESSION   DAOOQX010000316 DAOOQX010000000
VERSION     DAOOQX010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58625_length_2266_cov_2.868385"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="PLX53_05780"
     CDS             complement(<1..1363)
                     /locus_tag="PLX53_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY72628.1"
                     /translation="MSRNYHNLTSYYNYYYNAYDSYRNGERRVYKDFKYNYTLPLPML
                     LFGERGVSDMVSSDMERTLNKCTALLAKHSITVKPKRKNGSLSSKEKEFYNRYEYVRW
                     AQESWLLVGKAHTWKGDLSKAQMAFEYVIRQFPNTTTWYEAQVWLARVEILNGDFISA
                     GDRLKSVDAKRRKPTDRQFNHLLSSTWAFYYLKQHNSNEAIPFIKRAAEFTNYKAERL
                     RYTYLLAQLQQQLGKTTEAIELYDRVMRMGPPYEMAFSIRIQLIALSNMKGEDLKREL
                     LKLASDEKNKDYLDQLYYTLGNMEKQEGNMDKAIEYYLLSARLSTTNDNQKGMSYLVL
                     ADHYFQKSEYAKAQAYYDSSFNSLDASFPGYAQLETKTRFLTNLVENLNIVSTEDSLQ
                     RVAAMSPKDRDALIASIIGKLQEEEQKLKAQEQADQMQSQIYMQNQRFRSNSEPQGGK
                     WYFY"
     gene            complement(1793..>2266)
                     /gene="purQ"
                     /locus_tag="PLX53_05785"
     CDS             complement(1793..>2266)
                     /gene="purQ"
                     /locus_tag="PLX53_05785"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HOY72629.1"
                     /translation="ASNGGYVFGICNGFQILCEAELLPGALLHNASRQFVCKNVYIAP
                     DSKIAPPTAFLPKGRVFKIPIAHGEGRYFADRETLARMRVNGQIIFRYCDETGVISEL
                     ANPNGSVENIAGVCNERKNVFGMMPHPERASDDELGNTDGRLIFESIIKFIQGNG"
BASE COUNT          592 a          513 c          518 g          643 t
ORIGIN      
        1 tataaaagta ccatttcccg ccttgaggct cggagttgct tctgaatcgt tggttctgca
       61 tgtagatttg cgactgcatc tgatcggcct gttcctgggc ctttagcttc tgctcctcct
      121 cctgaagttt accaatgatg gaggcaataa gtgcatccct gtcttttgga gacatagcag
      181 caacccgttg aaggctgtcc tcggtgctaa caatgttcag gttttcaacc agattggtta
      241 gaaaccgggt tttggtttcc agctgtgcgt atccggggaa acttgcatcc aaggagttga
      301 atgaactgtc gtagtaggct tgtgccttgg catactccga tttctgaaag tagtggtcgg
      361 ccagcaccag gtacgacatg cctttctggt tatcgttagt ggtgctcaac ctggcagaaa
      421 gcaggtagta ctcaatagct ttatccatgt tgccttcctg cttttccatg ttacccagcg
      481 tgtagtaaag ctgatcgaga taatccttgt tcttttcatc cgaggcaagc ttcaggagct
      541 cacgtttcaa gtcttcaccc ttcatgttgc taagggcaat cagttgaatt cgtatgctaa
      601 atgccatttc gtagggtggc cccatccgca tcaccctgtc gtaaagttct atagcttcgg
      661 tagttttgcc aagctgctgc tgcagctgag ctagcaggta ggtataacga agccgttcgg
      721 ctttataatt tgtaaactca gctgcacgtt taatgaaagg aatggcctcg ttgctattat
      781 gctgttttag atagtagaat gcccatgttg atgatagcag gtggttaaac tgcctatctg
      841 taggttttcg tctttttgca tcaacagact tcagcctatc accagcgcta ataaagtcac
      901 cgttcaaaat ttcaaccctg gctagccata cctgagcctc gtaccatgtt gtagtgtttg
      961 ggaactgccg gataacatac tcaaaggcca tttgggcttt ggacaggtcg cccttccacg
     1021 tatgtgcctt gccaaccaaa agccatgatt cctgtgccca cctaacatac tcgtaccgat
     1081 tataaaattc cttttccttg gacgaaagtg aaccgttttt acgtttaggc tttacagtta
     1141 ttgagtgctt ggcaagtaat gctgtgcatt tgttcagggt tcgctccatg tcggaggaaa
     1201 ccatatcgct aacaccacgc tctccaaaca gcaacatggg aaggggaagc gtgtagttat
     1261 acttaaaatc cttgtaaact cgcctctcgc cgttacggta gctatcgtat gcgttatagt
     1321 aatagttgta gtaggaggta aggttatggt aattcctgct cataaaggta tttttctggg
     1381 ttgagcaaga agcaagaact aaagtcagca tgcaagctgc catgtttctg gtcaataagc
     1441 gcatagtacc agctgtttcc ccaatcaaaa gcaaagtatg ccgagttgaa ttaaacggaa
     1501 tgttgaaatt tatatagccc acaatattag caataaaagt tgaattgccc tccagggcag
     1561 ccacgagctt agcaacaatt cagctgtcca acaaacagta aacttttatt ccatcaaaca
     1621 accataagat ggtaggaaac cgttaagaac agccacagtt caaaaagata ttgtatgcta
     1681 aaaacgtttg tggcggtaaa ctattgttgt ggggtaaaca aaccttccat ctcggtctga
     1741 aagagggact gccgggttgt aacaaaaatg gaggaattat caaatttcca ggctagccgt
     1801 taccttgaat gaacttaatt attgattcga agataaggcg gccatcggtg ttaccaagct
     1861 cgtcgtccga ggctctttcg ggatgaggca tcatcccaaa aacgttcttg cgttcgttgc
     1921 aaacaccagc aatgttttca accgagccat taggatttgc caattcggag ataaccccgg
     1981 tttcgtcgca gtagcgaaaa ataatctgcc cgttcacccg catacgggca agtgtttccc
     2041 tgtcggcaaa gtagcggcct tcgccatggg caattgggat tttaaagact cgccccttgg
     2101 gaagaaatgc agtagggggt gcaattttgc tgtcgggagc gatgtataca ttcttgcata
     2161 caaactgacg gcttgcatta tgtaacaaag cacccggtag taattctgct tcacagagta
     2221 tctgaaaacc gttgcaaatc ccaaaaacat agcccccatt gcttgc
//
LOCUS       DAOOQX010000317         2262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_58771_length_2262_cov_2.513367, whole genome shotgun sequence.
ACCESSION   DAOOQX010000317 DAOOQX010000000
VERSION     DAOOQX010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58771_length_2262_cov_2.513367"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PLX53_05790"
     CDS             <1..730
                     /locus_tag="PLX53_05790"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOY72630.1"
                     /translation="IGKGAAIVAETGIPCISDFRAADICRNGQGAPLVPIGDKLLFTS
                     YDICLNLGGIANISFDNVDNERVACDVAPCNMLLNFLSLQLGMEYDAYGNAGAKGMVN
                     HDLLNRLNELEFYGLLGPKSLGREWFEQKVVPVFASCNASLQDKLRTAYEHIAIQISK
                     STAEISPGAMLVTGGGARNRFLVQLITEKVKHRVVVPENMLVDFKEALIFAFLAALFL
                     NGTPSALASSTGATADSISGCLYL"
     gene            830..>2262
                     /locus_tag="PLX53_05795"
     CDS             830..>2262
                     /locus_tag="PLX53_05795"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOY72631.1"
                     /translation="MRKLAVQIFTLVSFAFLADTGWACTNFLVTRGASTDGSTFITYS
                     ADSHYLFGELYFRPAADYPAGTMMKIYEWDTGKYLGEIPQVGHTYSVVGNMNEHQVSI
                     GETTYGGRLELQDSTALIDYGSLMYIALQRSKSAREAIKVMAELVEQYGYYSEGESFS
                     VADKNEVWIFEMIGKGTNLKVDKKTKKSYNADKGAVWVAIRIPDGYISGHANQARITT
                     FPQENLKNSISSRNLDKIFDPGVEVVYSSDVIDFARTKGYYTGTNEDFSFSDTYAPLD
                     FGAARACEIRVWSFFKEVNQSMWDYFDYAKGHNLKHRMPLYIKPERKISVKDMMNFMR
                     DHLEGTELDMRKDPGAGPHAMPYRWRPMEWKYEGKEYVNERATATQQTGFSFVAQARN
                     WLPDPIGGIFWFSVDDVASTAYFPAYCGITAVPESYAEGKGDMLTYCDHCAFWTFNKV
                     SNFAYLRYDLMSQDIKKVQDELEGRFI"
BASE COUNT          566 a          527 c          572 g          597 t
ORIGIN      
        1 aattggcaag ggtgctgcta tagttgcaga aacgggcatt ccgtgcattt ccgattttcg
       61 cgctgctgac atttgtcgaa atggacaggg tgcacccctt gttcccattg gtgataagct
      121 gcttttcacc agttatgaca tctgcctgaa ccttggagga atagccaaca taagcttcga
      181 caatgttgac aacgaaaggg tggcctgcga cgttgcacca tgcaacatgc tgctcaactt
      241 cctttcgctc cagctgggaa tggagtatga tgcttatggc aatgctggtg caaaagggat
      301 ggttaaccat gacctgctca accggcttaa tgagctggag ttttacgggt tgctcgggcc
      361 gaaatcactc ggtagggagt ggtttgagca aaaggtagtt cctgtttttg caagctgcaa
      421 tgcatccctt caggataagc tacgtacagc ctacgagcat attgccattc agatttctaa
      481 atcaacagca gaaattagtc caggtgctat gctggttacc ggcggtggcg ccaggaatag
      541 atttcttgtg cagctcatta cagaaaaagt gaaacatcgc gtggtagttc cagaaaacat
      601 gctggttgat tttaaggaag ctttgatttt tgcctttctt gcagcccttt tccttaacgg
      661 taccccttcg gcgcttgcct catcaaccgg tgctactgcc gattccattt ccggatgtct
      721 atacctataa ttctgatggt gaagaatatg gttgattaaa ccttcatcct gcagaaaaat
      781 cgaaataaat gctaggtttg caatttgcta attcttaaaa tgacaagcta tgagaaaact
      841 tgctgttcaa atttttactc tggtatcctt tgcttttctt gctgatacgg ggtgggcgtg
      901 cactaacttt ttggtaacca ggggtgcctc aaccgacggg tcaaccttta ttacctactc
      961 tgccgattcc cactacctgt ttggcgagct ttacttccgt cctgcggccg attaccctgc
     1021 aggcacaatg atgaaaatat acgagtggga caccggtaag tatttgggtg aaattcccca
     1081 ggttggccac acctactcgg tggtaggaaa catgaacgag catcaggtat ccattggtga
     1141 aaccacctac ggggggcgcc tggagcttca ggacagcaca gcattaatcg actatggatc
     1201 gctgatgtac attgccctgc aacgttccaa gagcgctcgt gaagccatca aggttatggc
     1261 agagctggtg gagcagtacg ggtactacag cgagggggaa tcgttctctg tggctgataa
     1321 aaatgaggta tggatttttg aaatgattgg aaaggggact aacctgaagg ttgacaagaa
     1381 aacaaagaaa tcctacaatg ccgataaggg agctgtttgg gtagccattc gtattcccga
     1441 cggctacatc tcgggacatg ccaaccaggc ccgcatcacc accttccctc aagaaaatct
     1501 gaaaaattct ataagctcca ggaaccttga caagattttc gatccgggtg ttgaggtggt
     1561 ttactcttct gatgtcattg acttcgcccg taccaaggga tactataccg gcacaaatga
     1621 ggattttagc ttttccgata cctatgctcc gctcgatttt ggtgctgccc gtgcttgcga
     1681 gattcgggta tggtcgtttt ttaaggaggt aaaccagtcc atgtgggatt acttcgacta
     1741 tgccaaaggg cataacctaa aacatcgtat gccgctctac atcaagcccg aaaggaagat
     1801 ttccgttaag gacatgatga acttcatgcg cgaccacctg gagggaacgg agctcgacat
     1861 gcggaaggat cctggagctg gccctcatgc catgccatat cgctggcgtc ctatggagtg
     1921 gaaatacgag ggtaaggagt acgttaacga acgcgcaacc gccacccagc aaactggctt
     1981 ctcgtttgtt gcacaggctc ggaactggct gcccgaccca attggcggta ttttctggtt
     2041 cagtgttgac gatgtggcct caaccgctta tttcccggca tactgcggta taacagctgt
     2101 tcccgaatcg tacgccgagg gtaagggcga catgctcacc tactgcgacc attgtgcttt
     2161 ctggactttt aataaggttt ccaacttcgc ctaccttagg tacgacctga tgagccagga
     2221 tattaaaaag gtgcaggacg agctggaagg caggttcatt ag
//
LOCUS       DAOOQX010000318         2262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_58777_length_2262_cov_2.157227, whole genome shotgun sequence.
ACCESSION   DAOOQX010000318 DAOOQX010000000
VERSION     DAOOQX010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58777_length_2262_cov_2.157227"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLX53_05800"
     CDS             <1..583
                     /locus_tag="PLX53_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY72632.1"
                     /translation="EISCMVYKENYINNVAGVNLTSDSTVTVLLYANRYTATFFVTSK
                     NVPVYNAQVIVEGIDTAYTSATGYANINLIPFGSNIRYSVKLNGYNTYLGTFNLEDRN
                     IVVNVPLTLTTIEPTKTNSFAIYPNPSNGNFSINLEGSFSVAIYNLAGYKVYLSNHAE
                     NSLQVDIHSLPAGIYIVRVTGKNFSTVAKIIKK"
     gene            1066..>2262
                     /locus_tag="PLX53_05805"
     CDS             1066..>2262
                     /locus_tag="PLX53_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOY72633.1"
                     /translation="MKETPIPREIVKQKIAESNLPRVGRGSIREIVHLVNEIEKASGQ
                     KFIRMEIGDPGLQPAEIGIEAEISALRSGVASKYPLVDGIPPLKKEISRFVKNFINID
                     VDERGCIPTVGSMQGGMAAFLMTTRCNTRKNTVLFIDPGFPVQKQQLKVLGIDYETFD
                     VYNFRGDKLHDKLESYLKKGNISTILYSNPNNPSWICFTEKELRIIGELATAYDAIVV
                     EDLAYLGMDFRKDLSKPGQPPYQPSVANYTNNWLMLISSSKIFSYAGQRIGCIVVSNE
                     LYNRHFPDLKRFFSSDEFGYAMIYGAVYPLSSGVCHSAQYGFAAMLKAANDGVYNFVN
                     DVREYGEKAKVMKKLFTDNGFKIVYDRDEGEPLADGFYFTISYPGLTGEELIEEMLYY
                     GISAISL"
BASE COUNT          692 a          510 c          482 g          578 t
ORIGIN      
        1 ggaaatatcc tgcatggttt ataaggaaaa ctacatcaac aatgttgcag gcgttaactt
       61 aacctccgac agcactgtta ccgttttact ttatgctaac cgctacaccg ctactttctt
      121 cgtaaccagc aagaacgtgc ctgtttacaa tgcccaggtg attgttgagg ggattgatac
      181 tgcttacacc agcgctacag ggtatgccaa tatcaacctt ataccatttg gtagcaacat
      241 tcgctactca gttaagctca acggctacaa cacctacctt ggaacgttta acctggagga
      301 tagaaatatt gttgtgaatg taccgctaac cctcaccacc atagaaccca ccaaaactaa
      361 ctcgtttgca atctacccca acccttcaaa cgggaacttc agcataaacc ttgaaggtag
      421 ctttagcgtt gccatttaca acttggcagg atacaaggtt tacctcagca accacgccga
      481 gaactcccta caggttgaca tacacagcct gcctgcaggt atatacattg ttagggtaac
      541 cggtaaaaac ttttcaacgg ttgcaaagat tatcaagaag taaagcaaat ggctacgaaa
      601 agctaaaagg gcagcaatgc ccttttttag ttttaggtat aaaatgtttc gaccacccca
      661 catgggttgt gccaggtata agaaagaata gctgtatatt tacattttat aacctttcct
      721 atccaaattt gacaaaaaga gttaaaaaac atcaccaatt ggcaataaat tgttacagaa
      781 aagtgaactt tcttttaaga gttaataatt ttgattaaca ttgtggcata aaaaggaacc
      841 gtaaaaagcg ctcattctcc aggtatattt tggtatggcc atatctttta cttacgtaaa
      901 actagtgaag atgcaatacc agcgcacgga gtaaacgtat gatggttttc tgcatctaag
      961 aaagagccat gttggagcca aatgagatgt aatagccagt aaacatggaa gctgcaatgc
     1021 gatataagcc aatacgtttt taaacaccga aaaaacaata ccatcatgaa ggaaacgcca
     1081 ataccccgcg aaattgttaa gcagaagatt gctgaatcga atcttccccg ggtggggaga
     1141 ggttccattc gtgaaattgt gcatctggta aacgagattg aaaaggcctc aggacaaaag
     1201 ttcatccgga tggagattgg cgatcccgga cttcagcctg ctgaaattgg aattgaagcc
     1261 gagattagcg ctcttcgaag tggggtggcc tcaaagtacc ccttggttga tgggatacca
     1321 cctctaaaga aggagatatc ccgctttgtg aaaaacttca tcaacattga tgttgatgaa
     1381 agaggttgca ttcccaccgt tggctccatg cagggaggaa tggcggcatt tctgatgact
     1441 acccgctgca atacccgaaa aaataccgtg ctctttatcg acccggggtt tccagtccag
     1501 aagcagcagc ttaaggtgct tggtattgac tatgaaacct ttgatgtgta taacttcagg
     1561 ggcgataagc tgcacgacaa gctggaatcg tacctgaaaa agggaaatat ttccaccata
     1621 ctctactcca atcccaacaa cccctcatgg atatgcttca cggaaaaaga gctaagaata
     1681 attggagaac tcgctactgc ctatgatgct atagttgtag aagacctggc ctacctcggc
     1741 atggacttta gaaaagacct gtccaaacct ggtcaaccac cctaccagcc cagtgtggcc
     1801 aactatacca acaactggct aatgctgatc tcaagctcaa agattttttc gtatgccgga
     1861 caacgaattg gatgcatagt ggtaagcaat gaactataca atcgccattt ccccgacctt
     1921 aaacggttct tctcgtccga tgagtttggc tacgccatga tatatggtgc tgtttacccc
     1981 ctatcgtcgg gtgtttgcca ctcggcacag tatggatttg cagccatgct gaaagcagca
     2041 aatgacggag tttacaattt tgtcaacgat gtaagggagt acggcgaaaa agccaaagtc
     2101 atgaagaagc ttttcaccga caacgggttt aaaatagttt acgacaggga tgagggcgaa
     2161 ccgcttgccg atggcttcta ctttaccata tcctaccccg ggctaaccgg cgaagagcta
     2221 attgaggaaa tgctctacta tggcattagt gccatttcgc tc
//
LOCUS       DAOOQX010000319         2256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_59010_length_2256_cov_2.841890, whole genome shotgun sequence.
ACCESSION   DAOOQX010000319 DAOOQX010000000
VERSION     DAOOQX010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59010_length_2256_cov_2.841890"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PLX53_05810"
     CDS             complement(<1..793)
                     /locus_tag="PLX53_05810"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOY72634.1"
                     /translation="MKTPYYQYDLQLLRQTLDAATREANKYGYTLHYAVKANANPVIL
                     NEISSYGLGADCVSGNEISAALSGGFNPKGIVFAGVGKTDEEIELAIARGIYCIHCES
                     VQELMTIQKIASAMQTTANVALRVNPNVDAHTHKHITTGTMANKFGLTVEEARRIIKN
                     SDRYSNLNLFGLHFHIGSQITEMNVFEELAQRVNELQHDFYANGKPAYLNLGGGLGIN
                     YQNPDSELIPPFMNYFDVFARNLNRNSWQSIHFELGRSLVGQCGKL"
     gene            complement(800..2158)
                     /locus_tag="PLX53_05815"
     CDS             complement(800..2158)
                     /locus_tag="PLX53_05815"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOY72635.1"
                     /translation="MKILKFGGTSVGDAQRIRKVAQLLPEAEPCIVVLSAMAGTTNAL
                     VEIANHSSSGNVSMAIEQAKQLEARYMETSHELFNSPVFRNRGEKFTNDMYAELYRKL
                     GCPPTKKVTLEIVALGEQLSTGLFHLYLTENRIRSTYLPALEFMRIDREREPDYFYIS
                     QSLERLIKANSFTRVFITEGFICRNAKGQTDNLGRGGSDYTAAIIGNVADSEEVQIWT
                     DIDGLRNNDPRHVVNTKPIRHLSYGEAAELAYFGAKILHPSTIQPCSEKNIPVVLKNT
                     LQPDDPGTLIDSVYIPKGIKAIAAKKGITAITIQSSRMLLAYGFLSHVFEVFNRYKTP
                     VDMIATSEVSISLTIDDCSNLQGIANELAEFSTVECTYDQTIVCIVGDFGANRKGYAA
                     RIFNALHEIPIRMISYGGSSNSISILVDSQSMTRTLQLLQEVVDADVDVNLEQTANTI
                     SY"
BASE COUNT          528 a          513 c          540 g          675 t
ORIGIN      
        1 caagcttgcc gcactggccc accaacgagc gccccagctc gaagtgaatg ctttgccagg
       61 agttcctgtt aaggttccgt gcaaaaacat caaagtaatt cataaatgga gggataagct
      121 ctgaatcagg attctggtag tttattccca accctccgcc caggttgagg taggcaggct
      181 taccattggc gtagaagtcg tgttgaagct catttactct ttgtgccaac tcctcaaaca
      241 cgttcatttc ggtgatttgc gaaccaatat gaaagtgaag cccaaagagg ttaaggttgc
      301 tgtagcggtc agaatttttt ataattcgcc tggcctcttc caccgtaagt ccaaacttat
      361 tggccatggt tccggtagtg atatgcttgt gggtgtgggc atcaacgttg ggatttaccc
      421 taagggccac gttggcggtt gtttgcatgg cgcttgcaat tttttgaatg gtcattagct
      481 cctgaaccga ttcgcagtgt atgcagtata tgcccctggc tatggccagc tcaatttcct
      541 catcggtttt acctacaccg gcaaacacaa tgcctttagg gttaaaccca cccgatagtg
      601 ccgcggaaat ctcattaccg ctcacgcaat cggcaccgag tccgtacgat gatatttcgt
      661 tgagtattac cggattggca tttgccttca ccgcatagtg tagggtgtag ccatatttgt
      721 tagcttctct tgttgctgca tcgagtgttt gtcttaaaag ctgcaggtcg tactggtagt
      781 aaggtgtctt catggtgtgc tagtatgaaa ttgtattcgc tgtttgttca aggttaacat
      841 ctacatctgc atcaacaacc tcctgcagca gctgcagggt tctggtcatt gattgggaat
      901 caaccagaat cgaaatgctg ttgctacttc ctccgtatga tatcatcctg atgggaattt
      961 cgtgcagtgc attgaagatt cgggctgcat agcccttgcg gttggcgcca aaatcgccaa
     1021 caatgcatac tatagtttgg tcataggtac attcaaccgt tgaaaattct gccagctcgt
     1081 tagcaatacc ctgtaggttg gagcaatcgt caatggttag cgagatagaa acctcagagg
     1141 tggcaatcat gtcaacagga gttttgtaac ggttgaacac ctcgaataca tggctaagaa
     1201 acccgtatgc cagcagcatt ctcgacgatt ggatggtaat ggcggttatg ccttttttag
     1261 cagctatggc cttaattcct tttggtatat agaccgagtc gataagggtt ccggggtcgt
     1321 cgggctgaag ggtattcttc agcaccacgg ggatattctt ttcgctgcag ggttggatgg
     1381 ttgacgggtg tagtattttc gcaccgaagt aggccagctc ggcagcttct ccgtacgata
     1441 ggtgcctaat tggtttggtg ttaaccacgt ggcgggggtc gttgttgcga agcccatcaa
     1501 tgtctgtcca aatctgcacc tcttccgaat cagctacatt tcctattatt gcggcggtat
     1561 agtcgcttcc acctctaccc aggttatcgg tttggccctt ggcattccta cagataaatc
     1621 cttcggtaat gaatacccgg gtaaaggaat tggctttgat taatcgttcc aggctctggg
     1681 aaatgtagaa gtagtcgggt tctctctccc tgtcaatcct cataaactct aaagcaggaa
     1741 ggtaggttga gcggattctg ttttctgtga ggtagaggtg gaacaaaccc gtggaaagct
     1801 gctcgcctag ggcaactatt tcaagcgtaa ctttttttgt aggcggacaa ccaagctttc
     1861 tgtaaagctc ggcatacata tcattagtaa acttttctcc tctattcctg aaaaccggcg
     1921 aattgaaaag ctcgtgagat gtttccatgt atcttgcttc gagctgtttg gcctgttcta
     1981 tcgccatgct aacatttcca ctactactat ggttagctat ctcaaccaag gcattcgtgg
     2041 ttcccgccat ggccgatagc accacgatgc agggttctgc ttcaggtagc agctgcgcta
     2101 ctttccttat tcgttgggca tccccaaccg atgttccacc aaatttgagt attttcattt
     2161 ctgtgctttt aggatttaaa aaataaaaaa aggcctgcat tgtttgcagg ccttttaatc
     2221 gcttagtttt tagtatatta ccacaaagtg tacttt
//
LOCUS       DAOOQX010000320         2255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_59058_length_2255_cov_2.690000, whole genome shotgun sequence.
ACCESSION   DAOOQX010000320 DAOOQX010000000
VERSION     DAOOQX010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59058_length_2255_cov_2.690000"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1108
                     /gene="floA"
                     /locus_tag="PLX53_05820"
     CDS             128..1108
                     /gene="floA"
                     /locus_tag="PLX53_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137107.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOY72636.1"
                     /translation="MSQVGAILTIIAVCIVGLWIIFYFVPVGLWFQALVSGVRISLLQ
                     LIFMRWRKVPPRVIVQAMIEGTKAGLTLNRNEMEAHYLAGGRVPKVVHALVSAQKANI
                     PLDFKMATAIDLAGRDVFEAVQMSVNPKVINTPPVTAVAKDGIQLITKARVTVRANIK
                     QLVGGAGEETVLARVGEGIVTAIGSSQNHKEVLENPDKISKTVLNKGLDAGTAFEILS
                     IDIADIDIGRNIGAVLQMDQANADKNIAQAKAEERRAMAVALEQEMKAKAQEARAKVI
                     EAEAQIPLAMADAFKSGNLGIMDYYRIKNIEADTNMRDSIAKPGVEPKKK"
     gene            1558..>2255
                     /locus_tag="PLX53_05825"
     CDS             1558..>2255
                     /locus_tag="PLX53_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOY72637.1"
                     /translation="MKNILVVGAGGQIGSELVPYLRKIYGNEYVVAADINSNACIHLA
                     EEGPFEELDALKADRYLEIVKKYRIDTIFNLVALLSATGEKKPQLAWHVNMGALMNSL
                     EIARENSCAVFTPSSIGAFGPTSPLDNTPQDTVMRPTTIYGICKVTGELLSDYYFLKY
                     GVDTRSVRFPGIISNVTLPGGGTTDYAVEIFYGAVQHKHYVCPLPEGTYLDMMYMPDA
                     LDACVQLMEADPSKL"
BASE COUNT          644 a          544 c          509 g          558 t
ORIGIN      
        1 aagccaccat aaatggacat gacgttgagg ttaccgccct cgatgagcta gttgacccac
       61 aaacaacagt tgtagtagtt aaagttgaag gcagcaaaat aattgttaaa cctaaaaaat
      121 agattacatg agccaagttg gagcaatact taccattata gccgtctgca ttgtcggctt
      181 atggattatt ttctactttg taccggtagg tctgtggttt caggcgcttg tttccggtgt
      241 cagaatctca ctcctgcagc taattttcat gcgctggcgg aaggttcctc cccgggtaat
      301 tgtgcaggcc atgattgagg gaactaaagc cgggcttacc ctgaaccgta atgaaatgga
      361 agcccactac cttgccggag gaagggttcc gaaggtggtt catgcacttg tttcggccca
      421 aaaagcaaac atcccgctcg acttcaaaat ggcaaccgct attgacctgg ctggccgtga
      481 tgtttttgaa gccgttcaga tgtccgtaaa ccccaaggtg ataaataccc caccggttac
      541 cgctgtagcc aaggacggca ttcagcttat taccaaggca agagttaccg ttagggctaa
      601 cattaagcag ctggttggtg gtgccggtga agaaactgtt ctggcccgtg ttggcgaggg
      661 aatagttacc gccattggtt cgtcgcaaaa ccacaaggaa gttctggaga accccgataa
      721 gatttccaaa acggttttga acaaagggct ggatgccgga actgcctttg aaatcctttc
      781 tattgatatc gccgacattg atattggacg caatattggc gctgtactcc agatggacca
      841 ggccaacgcc gataaaaata ttgcccaggc caaagcagaa gagcgtaggg ctatggccgt
      901 tgccctggaa caggaaatga aggctaaagc acaggaggcc cgcgctaagg ttattgaagc
      961 cgaggctcaa attccattag ccatggccga tgcttttaaa agcggaaacc tgggtatcat
     1021 ggattactac cggataaaaa atattgaagc cgataccaac atgcgcgaca gcatcgcaaa
     1081 acccggagtt gaacccaaaa agaagtagat agcataacat cctaaaatag cttgtgcaaa
     1141 aaagagggga gttgcccctc tttttctttt caaccccacc cgtaaaaaac ctaccaccta
     1201 ccctgacctc gacttggttc cgaatgctga ttaaaaatca attaaggtgg cattgaatct
     1261 ggctgttaaa aatgctattc cttacccaaa ttccaaaaat aaccatcctg atgtctaaaa
     1321 aaatatttac caaaatatgc taaataccat acaggctatt aagcaaaagc tatacttttg
     1381 ctcaacattt taatcaatat caccaatttg gtaaaaattt gatagaaaaa ggcattcatc
     1441 cggggtagca attcaagcat tgctttaccc gttgactttt ctttcggatt ttttaacgag
     1501 aatggctaca taacttcata acatattgtt aaatagcaag gtaactatac taaaaaaatg
     1561 aagaatattc tggttgtagg cgccggcggg caaattggct ccgaactggt tccctatctt
     1621 agaaaaattt acggaaacga gtatgttgtg gcagctgata tcaactccaa cgcttgcatt
     1681 cacctggccg aggaaggtcc ttttgaggag ctggatgccc taaaggccga caggtatctc
     1741 gaaattgtaa agaaataccg gatagatacc atattcaacc tggttgcact actctccgcc
     1801 accggcgaaa aaaaacccca gctggcctgg catgttaaca tgggcgccct gatgaactcc
     1861 ctcgagattg ccagggagaa cagctgcgcc gtttttaccc ccagctctat cggcgcattt
     1921 ggcccaacat cccccctcga caacacccca caggataccg tgatgcgccc cacaacaatt
     1981 tacggcatct gcaaggtaac cggcgagctg ctgagcgatt actacttcct gaagtatggc
     2041 gttgataccc gtagcgttcg ttttcccgga ataatatcta atgttaccct tcccggtgga
     2101 ggtaccaccg attacgctgt ggagattttc tacggtgcag ttcagcataa gcactacgtt
     2161 tgccctttgc ccgagggaac ctacctcgac atgatgtaca tgcccgatgc tcttgatgcc
     2221 tgcgtgcagc ttatggaagc cgacccctcg aagct
//
LOCUS       DAOOQX010000321         2254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_59086_length_2254_cov_3.140518, whole genome shotgun sequence.
ACCESSION   DAOOQX010000321 DAOOQX010000000
VERSION     DAOOQX010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59086_length_2254_cov_3.140518"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="PLX53_05830"
     CDS             <1..676
                     /locus_tag="PLX53_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOY72638.1"
                     /translation="DLLRKLAEVAPGTFQHSLQVANLAETAIQHVGGNPLLVRAGVLY
                     HDIGKMANPLYFIENQPYDFNPHRNLDFTESASIIINHVTEGVRIGRKAGLPDQLIDF
                     IQTHHGTSKVRYFYQRHLDKFDNGAADAVKFTYPGPIPPSKETAILMMADAVEAASRS
                     MKNVTEENIKELVDTLINQQLADGQFNDANITFKDISTIKSVFKKKLLNIYHARIEYP
                     ETKANE"
     gene            complement(765..1175)
                     /locus_tag="PLX53_05835"
     CDS             complement(765..1175)
                     /locus_tag="PLX53_05835"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOY72639.1"
                     /translation="MDKLAELFELEMEVRDYECDLEGIVNNAVYLNYLEHARHKYLLS
                     WGIDFARLHTEGIDLVVTRIEIDYKRSLASGDKFKVRVSARREGHLRLVFDQEVIAIP
                     DGSTAVKAKVIGVGLKQGRPVKIEGIPGFENFSA"
     gene            complement(1246..2034)
                     /locus_tag="PLX53_05840"
     CDS             complement(1246..2034)
                     /locus_tag="PLX53_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOY72640.1"
                     /translation="MTNNPTDTQVIQDCTILSIRNLNVHAGNHHILKDINLEIPKNRI
                     TVLLGPSGCGKTTLLKCMNKLTDLYRELKVTGNIFIEDDDILNTSKNIPSIRQKMGLL
                     SQRPYPLPVSIYKNVAYGIKLKGIRDRKLIDYNVEKHLKEVGLWNEVKDRLHSPATSL
                     SIGQQQRLCLARGLAVKPRIILADEPTSALDPISSKIIENLFRNLKENYTIILVTHVL
                     RQAIRLADHVIFMYNGEIIEQGTPDELFNNPKSEKLKEYMVEGN"
     gene            complement(2059..>2254)
                     /locus_tag="PLX53_05845"
     CDS             complement(2059..>2254)
                     /locus_tag="PLX53_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease"
                     /protein_id="HOY72641.1"
                     /translation="DLIPVNLDEPAATLPLAIFFQLGSPIPEVRERAYAASTILTIII
                     LIISLSARFLSNRYSKTNIK"
BASE COUNT          618 a          550 c          483 g          603 t
ORIGIN      
        1 cgatttactt cgaaagctgg ccgaagttgc cccaggcacc tttcagcact cccttcaggt
       61 ggccaacctg gctgaaacag caattcaaca tgttggcgga aaccccctgc tggttagggc
      121 tggagttcta taccatgata ttgggaaaat ggccaacccg ttgtacttca tcgaaaacca
      181 accatacgac ttcaaccccc accgaaacct ggattttacc gaaagcgcat caatcattat
      241 caaccatgtt accgagggtg tcagaattgg ccgcaaggcc ggacttcccg accagctgat
      301 tgacttcata caaacccacc acggcacctc aaaggttaga tacttctacc agcgacacct
      361 cgataagttt gacaacggcg ctgccgatgc cgtaaagttt acctaccctg gccctattcc
      421 gcccagcaag gaaacagcca ttctgatgat ggctgatgca gtggaagccg cctcaaggag
      481 tatgaagaat gtgactgagg aaaacataaa ggagcttgtt gacacgctta taaaccagca
      541 gctggccgat ggtcagttta acgatgccaa catcaccttt aaggatatct cgaccattaa
      601 atcggttttc aagaagaagc tgctgaacat ctaccacgcc cgaatagagt accccgagac
      661 taaggctaac gagtaatttt tttactttcg gatagtagta ggttgattct cgcaggcact
      721 tatggcccac ggtaacaacc ataaaagcaa cataaccaag cgaactaggc cgaaaaattt
      781 tcaaaacccg gtatgccctc aatcttaact gggcgaccct gttttaaccc tactcctata
      841 accttcgcct ttactgccgt tgagccatcg ggtatggcta tcacctcctg atcaaacacc
      901 aaccgtaaat gcccctcacg acgagctgat acccttacct taaatttatc accgctggcg
      961 agcgaacgtt tgtagtctat ctctattcgg gtaaccacca aatctatacc ctcagtgtga
     1021 aggcgagcaa agtcaatacc ccagctgagc aaatatttat gacgggcatg ctccaggtag
     1081 ttcaggtaaa ctgcgttgtt tacaattccc tcaaggtcgc actcgtagtc ccgaacctcc
     1141 atctctagtt cgaacagctc tgctaatttg tccatggtat gtaacaaata tttatacggc
     1201 cattgaataa cactctgaag agtagcgcaa atttggtaaa aaaaatcagt ttccctcaac
     1261 catgtactcc ttaagcttct ccgatttagg gttattgaag agctcatcgg gtgttccctg
     1321 ttcaataatt tcaccattat acataaaaat tacatggtcg gccaaccgaa tagcctgcct
     1381 aagtacatgc gttaccagga taatggtgta attttccttg aggtttcgga acaggttttc
     1441 gataatttta ctggaaatgg ggtcaagggc cgaggttggc tcatcggcca ggattattcg
     1501 gggtttcacc gccaaacctc gcgccaaaca aagcctttgc tgctgaccaa tggagaggct
     1561 ggtggctgga gagtgcagcc tatccttaac ctcgttccac aagcccacct ccttaaggtg
     1621 cttttccaca ttgtagtcta tcagctttcg gtcgcgtatc cccttaagct ttatcccgta
     1681 ggctacattc ttataaattg aaacgggtaa cgggtagggc cgttgcgaga gcaaacccat
     1741 tttttgccta attgaaggta tatttttact tgtattcagg atgtcatcgt cctcgataaa
     1801 aatgttgccc gtgaccttaa gctccctgta caggtcggtt agcttattca tgcactttag
     1861 cagggtggtt ttgccacaac ccgaaggacc tagaagaact gttatcctat ttttaggaat
     1921 ttccaggttt atatccttca gaatatgatg gttacccgca tgtacgttta ggtttctgat
     1981 gcttaaaatt gtacagtcct gaataacttg agtatcagta gggttattgg tcattttaat
     2041 atcaatttct aagatgaact acttaatgtt agtttttgaa tatcggttcg aaaggaacct
     2101 tgcgctaagg cttataatca ggatgatgat ggtgagaata gtgcttgcag cataggcacg
     2161 ctcccttacc tcagggattg gagagcccag ctgaaagaat atagccaaag gcagcgtggc
     2221 ggcaggctca tcaaggttaa caggtattaa atcg
//
LOCUS       DAOOQX010000322         2253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_59119_length_2253_cov_3.562329, whole genome shotgun sequence.
ACCESSION   DAOOQX010000322 DAOOQX010000000
VERSION     DAOOQX010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59119_length_2253_cov_3.562329"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PLX53_05850"
     CDS             complement(<1..721)
                     /locus_tag="PLX53_05850"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72642.1"
                     /translation="MGAFHAYDIRGIYNKDFNKDDAYKIGFFLVELLKTDRVLVGRDV
                     RMSSDEIFTYLTRGITDAGADVYNLGLATTPMVYYATAKGDFTASVQITASHNPKEYN
                     GLKVSGRNAEPIGYDTGLKFIEERMKSSSVVPVKDTGRVIDYDIRKDYITFLNSFKQD
                     YSNLKFAVDCSNGMAALLVKDILGNSPVYIYDELDGSFPNHEANPLIPENVVDLQNLV
                     RKQKCDLGIIFDGDADRVMFVD"
     gene            863..1564
                     /locus_tag="PLX53_05855"
     CDS             863..1564
                     /locus_tag="PLX53_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72643.1"
                     /translation="MRFRSKLIWVTLFAIAMGLLESAVVIYMRELLYPGGFSFPLSPI
                     PERLAVTELLRELATLVMLVGVGVVAGRNFPERFAWFIFSFGVWDIFYYVFLKLLIGW
                     PTSMLTWDLLFLIPVTWTGPVVAPVAISLMMITLALVILKSAGKNSGFRITAISWATL
                     IAGAMVVFLSFITDYTRFILHSFTLRELWSLPSKQALFDLSVQYIPFSFPWMIFAAGS
                     AVIIAAGIRIFNENR"
     gene            1769..>2253
                     /locus_tag="PLX53_05860"
     CDS             1769..>2253
                     /locus_tag="PLX53_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HOY72644.1"
                     /translation="MKRYSWILMTMIAIGAIVSCKSEKQTDNPFLEDYTTPFGVPPFD
                     KIRVEHFEPAIMEGIKQQLAEVEAITTNSEKPTFENTIIPLDYSGELLTKVVLTMENI
                     NSANTSEELQEMLKKVSPVLSKHRDDISMNPKLFERVKMVYSSMNESSYTPEQKKLIE
                     NT"
BASE COUNT          619 a          514 c          471 g          649 t
ORIGIN      
        1 catcaacaaa catcacccga tcggcatcgc catcgaaaat aatcccaagg tcgcatttct
       61 gctttctcac caggttttgc aggtcaacca cattctcggg tataaggggg ttagcctcgt
      121 ggttggggaa tgaaccgtcc agctcatcgt agatgtacac cgggctattg cccagtatgt
      181 cctttaccaa aagtgcagcc attccgttgg agcagtcaac cgcaaacttc aggtttgaat
      241 agtcttgctt gaagctgttg aggaaggtga tgtaatcctt ccttatatca taatctatta
      301 cccttccggt gtccttcacg ggtaccacac tggagctttt catcctctcc tcaatgaatt
      361 ttaaaccggt gtcgtagcca ataggctcgg cattcctgcc cgataccttc aacccgttgt
      421 actccttggg gttatgcgaa gcggttatct gtaccgatgc cgtgaaatct cccttggcgg
      481 ttgcgtagta aaccattggc gtggtggcta atcccaggtt ataaacatcc gcaccagcat
      541 cggttatacc acgagtcagg taggtgaaaa tttcatcgga cgacatgcga acatcacgtc
      601 ctaccagcac cctgtcggtt ttcaaaagtt caacgaggaa aaagccaatt ttataggcat
      661 catccttgtt aaaatccttg ttataaattc cacgaatatc gtaggcatga aaagcaccca
      721 tagtcctgtt taccggttaa gttatcatca atttactttg gctggcaaaa ttaaaaacat
      781 tattccttgt tgaaaccttt tggccataat attgaccaaa gtcagtaaaa aagagtaaat
      841 ttgataggtt aaacagaaaa ttatgcgatt ccgaagtaaa ttgatatggg ttacgctttt
      901 tgccatagcc atggggctac tggagagcgc cgttgtaatt tacatgcgcg agctcctata
      961 ccctggtggt ttttcctttc ctctctcccc aattcccgag aggttggcag ttaccgagct
     1021 gcttcgggag ttggcaaccc tggttatgct tgttggagtt ggcgtagtgg ccgggaggaa
     1081 cttccctgag cgttttgcat ggtttatttt cagctttggc gtttgggata tattttacta
     1141 cgttttctta aagctgctta taggctggcc cacctcgatg ctaacatggg atttgctatt
     1201 cctcattccg gttacgtgga ctggccccgt agttgccccg gtagccatat cccttatgat
     1261 gattaccctt gcgttggtta ttttaaaatc agcaggcaaa aactcaggtt tccgaataac
     1321 agcaatttca tgggcaactc taattgccgg agccatggtt gtattcctat catttattac
     1381 cgattacact aggtttattc tgcatagctt cactttacgg gagctatggt cgttgccatc
     1441 aaaacaagct ctgttcgatt tgagcgtaca gtacattccc ttttcattcc catggatgat
     1501 atttgctgca ggcagtgctg taataatagc cgcaggtatt agaattttta acgaaaaccg
     1561 atagcgttaa tagctaaaaa aacgtgaatg ccgtacaggc ttccggcaaa agccgtgcag
     1621 gtttagtttt ccttatcatt caccatcaat ctttcatttt tttaaatatg ccctgctcta
     1681 ctacttcatt cctaacttta tccattatct ttgcttgcag acaagtaaat agctattcgt
     1741 gttaacttaa attaaactat acaatcaaat gaaacgatac agctggattt taatgacaat
     1801 gatagccata ggcgctattg tctcatgtaa aagcgaaaag caaacagaca acccattcct
     1861 ggaggattac accacgccat tcggcgttcc accatttgat aagataaggg ttgagcactt
     1921 cgaaccggca atcatggagg gtattaagca acagctagct gaagttgaag ctattactac
     1981 taacagcgaa aaacccacat ttgagaatac cataattccg ttggactata gcggtgagtt
     2041 gctgacaaag gttgtgctga caatggaaaa catcaactcg gccaacacca gcgaagagct
     2101 gcaggagatg cttaaaaaag tttctccggt tctatcgaaa caccgcgacg atatcagcat
     2161 gaaccctaaa ctttttgaac gggtgaagat ggtttacagc agtatgaacg aaagcagcta
     2221 cacccccgag caaaaaaaac ttattgaaaa cac
//
LOCUS       DAOOQX010000323         2251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_59221_length_2251_cov_2.507286, whole genome shotgun sequence.
ACCESSION   DAOOQX010000323 DAOOQX010000000
VERSION     DAOOQX010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59221_length_2251_cov_2.507286"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PLX53_05865"
     CDS             complement(<1..504)
                     /locus_tag="PLX53_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOY72645.1"
                     /translation="MKKRFLALLTGLLLLTGAMVRADEGMWLLPLLKQLNIDVMQQKG
                     LKLSADDIYQINQSSLKDAVIIFGRGCTGEIVSDQGLILTNHHCGYGAIQQHSTPEHD
                     YLADGFWAKSLQEEIPTPGLTATFLVRIEDVTDQVNAALNPSMTEAERNQAIQAVGKK
                     KAGEKPAS"
     gene            complement(575..1180)
                     /locus_tag="PLX53_05870"
     CDS             complement(575..1180)
                     /locus_tag="PLX53_05870"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOY72646.1"
                     /translation="MGCLRTIHTPGLVEAGCDEAGRGCLAGPVFAAAVILPPDFYDNS
                     IDDSKKLTLKQRLAARQLIQHYALDWAVARVSNEEIDRLNILNASILAMHMAIKALTR
                     ATPQHLLVDGNRFKNFPGIPHTCIVGGDHTYQSIAAASILAKTYRDELMNELSEQFPG
                     YGWRTNKGYPTKLHRRAIAELGPTPYHRLTFALLPQSKKKC"
     gene            complement(1186..>2251)
                     /locus_tag="PLX53_05875"
     CDS             complement(1186..>2251)
                     /locus_tag="PLX53_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOY72647.1"
                     /translation="TYETFSRMAIDHPNDEFFFIFDRPFNREFIFAKNIHPVVLGPPA
                     RHPILWYIWFEVSVNRFLKKLKPDIFISPDGYLSLKANTPQLAVIHDINFYYYPKGLP
                     WLTSLYLNKYFPKFAGKAKRIVTVSEYSKNTLVEAYKVNPQKIDVVYNGASDNFKPLN
                     EIERADQRNQLTDGWPYFVFVGAFSPRKNISSLINAFDMFRRKTNIQAKLVLVGETMF
                     KASSIEAAHHKSKFKDDIIFTGRKGLPELSRIVGAAQALIFPSLFEGFGIPVLEAILC
                     EVPVAASNSTSMPEVAGNAAIYFEPTSDESIAEAMERIWMDTQLRQRLISNARVQRQK
                     FSWEQAASRLYDIIAKTIES"
BASE COUNT          556 a          512 c          539 g          644 t
ORIGIN      
        1 agaagcgggc ttttcgcccg cttttttttt gcctacagct tgaatggcct gattgcgttc
       61 tgcctcggtc atgctggggt ttagggcagc atttacctgg tcggtaacat cctcgatacg
      121 tactaaaaat gttgcggtta gccccggggt gggaatttct tcctgcaacg actttgccca
      181 aaatccatcg gcaaggtagt catgctcagg ggtgctatgc tgctggatgg ccccgtaacc
      241 gcagtgatgg ttggtaagaa tcaacccctg gtcggatact atttcgccgg tgcaacctcg
      301 accgaagatg attacagcat ctttaaggct cgactggtta atctggtaga tgtcgtcggc
      361 agagagctta agcccttttt gctgcatgac atcaatgttc agctgcttga gcaggggcag
      421 aagccacatc ccctcatcgg ccctgaccat tgcaccggtt aaaagcaaca aaccggttag
      481 tagtgcaaga aatctttttt tcatgttgtt tgagttttgg ttgttaatgg ttaagtatag
      541 tgttatgaca gactagctct tgtttagttt aaagctaaca ttttttcttg gactggggca
      601 aaagggcgaa cgtcagccga tggtagggcg ttggccctag ttctgcaatt gccctcctat
      661 gcagcttggt tgggtagccc ttgtttgttc tccagccata acctggaaac tgttctgaga
      721 gttcattcat tagctcatcg cggtatgttt tagcaagtat tgatgctgcg gcaattgact
      781 ggtaggtgtg gtctccaccc actatgcatg tatggggtat tcctggaaaa ttcttaaacc
      841 tattgccatc aaccagtaaa tgctggggag ttgccctggt aagagccttt attgccatgt
      901 gcattgccag aatggatgca ttaagaatgt tcaacctgtc tatttcctcg ttcgacaccc
      961 ttgccacagc ccaatctaat gcgtagtgtt gaattagctg ccgggcggca agccgttgct
     1021 ttagggttag cttttttgaa tcatcaatgg agttatcgta aaaatcggga ggcaaaatta
     1081 ctgctgccgc aaaaacaggc cctgccaggc aaccccttcc ggcctcatcg caacccgctt
     1141 caacgagtcc cggcgtgtgt attgtcctca aacatcccat tcccatcaac tttcgattgt
     1201 tttagcaatg atgtcgtaaa gcctgctggc ggcttgctcc cagctgaact tttggcgctg
     1261 cacccttgcg ttggagatga gtctctgcct tagctgggta tccatccata tacgctccat
     1321 tgcttcagca attgattcat cactggttgg ttcaaagtag attgcggcat tccctgctac
     1381 ctcgggcatt gaggtagaat ttgatgcggc aacaggaact tcacacagta tggcctccag
     1441 aacaggtatc ccaaaaccct caaaaagcga cggaaaaatc agggcttgag cagcgccaac
     1501 aatacggctt aactcgggca acccttttcg gccggtaaaa ataatatcat ccttaaattt
     1561 acttttatgg tgggctgctt ctatggaaga agctttgaac attgtttcac ccaccagaac
     1621 cagctttgcc tgaatgtttg ttttacgcct aaacatatcg aaagcgttaa tgaggctgct
     1681 aatatttttt cttgggctga atgcaccaac gaaaacaaag tagggccatc catcggtaag
     1741 ctggttgcgc tgatcggctc gttctatttc gtttaggggt ttaaagttat cactagcacc
     1801 attgtaaact acatctattt tttgcggatt taccttgtaa gcctcaacaa gcgtattttt
     1861 tgaatactca cttacggtga caattcgttt tgccttacct gcaaattttg ggaaatactt
     1921 gtttaggtac aacgaggtca gccatggcag tcccttgggg tagtagtaaa agttgatgtc
     1981 gtgaataact gccagctggg gtgtattggc tttaagcgac aggtagccat cgggagaaat
     2041 gaaaatatcg ggtttcagct ttttcaaaaa ccgatttacg gaaacctcaa accagatata
     2101 ccaaagaata ggatgccgtg caggcggacc aagcacaacg gggtgaatat ttttggcaaa
     2161 aataaactcc cggttaaacg gccggtcaaa aatgaagaaa aactcatcgt ttggatggtc
     2221 tatggccatg cggctaaagg tctcgtaggt a
//
LOCUS       DAOOQX010000324         2247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_59357_length_2247_cov_2.459398, whole genome shotgun sequence.
ACCESSION   DAOOQX010000324 DAOOQX010000000
VERSION     DAOOQX010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59357_length_2247_cov_2.459398"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PLX53_05880"
     CDS             complement(<1..907)
                     /locus_tag="PLX53_05880"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOY72648.1"
                     /translation="MKKLNGYLLAGVALLTFTVVSCEDFLDKDPISNYSNSTLGVDNN
                     TDTLKYTTAEQAEALLATCYSNFRGELFQLDYFVNGDMQSDDAYAGASGTEFSQFDNF
                     SIDATNKNVERDWKYLYKMVTDCNTVIVNVPKITDQALTDARKKEIVAEASFIRAWAY
                     FDLVRLYGDVPLVLDVVTTITADNVAEVYPLLYPKRDSKELVYDQIITDLNTALDGVP
                     VTAPNKGYVTKGAVNALLARVYATRQPIDYTKVNDYCDAVINGGYSLLSNYDYLWDFQ
                     HENSSEAIFEIQYDGNWNTGTGNWAV"
     gene            complement(920..>2247)
                     /locus_tag="PLX53_05885"
     CDS             complement(920..>2247)
                     /locus_tag="PLX53_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOY72649.1"
                     /translation="IASFFGRVSYNYMSKYLFTANFRYDGSSKLSSDNRWDFFPSFSA
                     AWRLSSEPFLKNVVFINDLKIRGGWGRNGNQEGIGDYAWQALYGFSRQSPTSPLSGPG
                     IYQMNLENPSLKWEVTYQSNVGLDLTVLDSHLSFTGDVYWKNTDGLLVNVPLPSGSAA
                     VDYITRNDGKMKNWGVELSLDAKDFRHELKWSTNLFWSMNRNEITELQLSQIYNFAQL
                     ESNNEYVIRMSPGYSLGTFYGYVAKGVDPETGDMIYEDRNNNGVIDPDDRTVIGSAEP
                     DFIYGLNQTLTYKGIGLTLFFQGSQGNDIFNASRIDTEGMFDVKNQTTEVLRRWKRPG
                     QITDIPRAVSNGSTFNVHNSTRFVEDGSYFRLKALTLSYSLPSKWLSRVSIKNFTLYA
                     TATNLFTITGYNGFDPEVNYGGNNSVIQGVDYGTYPQSRSYIFGLSFDF"
BASE COUNT          587 a          530 c          552 g          578 t
ORIGIN      
        1 taacagccca gttgcctgta ccggtgttcc agttaccgtc gtactggatt tcaaaaattg
       61 cctcactgga gttttcgtgc tgaaaatccc acaggtagtc gtagttgctt aacaggctgt
      121 agccaccgtt aatcacggca tcgcagtagt cgttcacctt tgtgtagtca attggctggc
      181 gggtagcata aacccttgcc agcaaggcat taacagcacc tttggttaca tagcccttgt
      241 tgggagcagt aaccggcaca ccatcaagcg ctgtattcaa atcggtaata atctgatcgt
      301 aaaccaactc cttggagtcc cttttagggt aaaggagggg gtaaacctcg gcaacattat
      361 cggctgtaat ggtggtaacc acatcgagca ccaggggaac atcgccgtaa aggcgaacaa
      421 ggtcgaaata tgcccatgca cgaataaacg atgcctcggc aacaatctcc tttttacggg
      481 catcggttaa tgcctggtca gtaatcttag gaacgtttac aattaccgta ttgcaatcgg
      541 ttaccatctt gtaaaggtac ttccaatcgc gctccacgtt cttgttggta gcgtcgatgc
      601 tgaagttgtc aaactgggaa aactcggttc cgctggcacc ggcgtaggca tcgtcggact
      661 gcatatcgcc gttgacaaag tagtcgagtt gaaacagctc gcccctgaag ttggagtagc
      721 aggtggccag cagcgcttct gcctgttcgg cagtagtgta ctttagagtg tcagtattat
      781 tatcaacgcc cagcgtgctg ttggagtagt tcgaaatggg gtccttgtcg agaaagtcct
      841 cacacgaaac caccgtgaat gttaaaagag caacgccggc aagcaggtat ccattaagtt
      901 ttttcatatc tctcggagtt taaaagtcga acgataaacc aaatatgtat gatctcgatt
      961 gcggataggt gccgtagtca acaccctgaa ttacgctgtt gtttccaccg tagttaacct
     1021 cgggatcgaa tccgttgtaa cctgtaatgg taaacaggtt ggtagcagtt gcataaaggg
     1081 tgaagttttt aatgctaacc ctgctgagcc attttgacgg taaggagtag gacagggtaa
     1141 gagccttaag cctaaagtat gatccatcct caacaaagcg ggtagagttg tgcacattga
     1201 aagtgctacc gttgctcaca gcgcggggta tatcggtgat ttgacccggc cgcttccacc
     1261 tgcgcagcac ctcggttgtt tgatttttca cgtcaaacat gccctcggtg tcaatccggg
     1321 aggcgttgaa aatatcattc ccctgtgaac cttggaaaaa cagggttaag cctattccct
     1381 tgtaggttaa ggtttgattt aagccgtaga taaagtcggg ttcagctgaa ccaataaccg
     1441 ttcgatcgtc agggtcaata acgccattgt tattcctgtc ctcataaatc atatcgccgg
     1501 tttcggggtc aactcccttt gccacatatc cgtaaaatgt tccaagggag taacccgggc
     1561 tcatccggat aacgtactcg ttgttgcttt cgagctgggc aaagttgtag atctgcgaaa
     1621 gctgaagttc ggtaatttcg ttgcggttca tcgaccaaaa aagatttgtg ctccatttca
     1681 gctcatgcct gaagtccttg gcatccagcg acagctccac gccccagttt ttcattttcc
     1741 catcgttacg ggtaatgtaa tcaactgcag cagagcccga agggagtgga acattcacca
     1801 gcaggccatc ggtattcttc cagtacacat cgccggtgaa gctcaaatgc gagtccagca
     1861 cggttaagtc gagcccaacg ttggactggt aagtaacctc ccatttaagc gaagggttct
     1921 ccaggttcat ctggtaaata ccgggtcccg aaagcgggct ggtgggggat tgacgggaaa
     1981 aaccataaag agcctgccat gcgtaatcgc caattccctc ctggtttcca ttcctacccc
     2041 aaccaccacg aattttcaaa tcgttaataa agacgacatt ctttaaaaat ggctcggacg
     2101 ataacctcca tgcagccgag aatgaaggga agaagtccca ccggttatcg gaggatagtt
     2161 tggaagaacc gtcgtagcgg aagttagcgg taaagaggta tttactcatg tagttatagg
     2221 atacacgtcc aaaaaatgat gcaattg
//
LOCUS       DAOOQX010000325         2245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_59439_length_2245_cov_3.157991, whole genome shotgun sequence.
ACCESSION   DAOOQX010000325 DAOOQX010000000
VERSION     DAOOQX010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59439_length_2245_cov_3.157991"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLX53_05890"
     CDS             <1..186
                     /locus_tag="PLX53_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOY72650.1"
                     /translation="KTLHEAVEHTGKGKPVIILMRTQMGMGVDFMVGNHKWHGKAPND
                     EEFAKAMAQLPETIGDF"
     gene            208..606
                     /locus_tag="PLX53_05895"
     CDS             208..606
                     /locus_tag="PLX53_05895"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOY72651.1"
                     /translation="MKNIALIAHDAKKPELARFLKDHEEWLPGVNLLATGRTAEFLEE
                     QGIKCRHLSPGQSGGYNEITGMIQRGEVDIVIFLRDPKVVQQHHEDIRLLLEACNIKN
                     IPLATNKATAELVILGLIHMEAIERMKRNK"
     gene            735..1691
                     /locus_tag="PLX53_05900"
     CDS             735..1691
                     /locus_tag="PLX53_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOY72652.1"
                     /translation="MKYTSTGNKDTRSGFGAGLYELGKANPNVVALCADLIGSLKMED
                     FSREFPDRFFQTGIAEANMIGLSAGLTIGGKIPFAGTFAVFATGRVYDQIRQSVAYSN
                     KNVKICASHAGITLGEDGATHQMLEDIGLMKMLPNMTVINTCDYNQTKAATIAIANHV
                     GPVYLRFGRPAVPNFTPADQQFTIGKAVKLTEGTQVSVFATGHLVWKALEAADMLEQQ
                     GISAEVVNIHTIKPLDEEAIIRSIQKTGCAVTAEEHMMNGGLGDSIAQVVARIHPVPL
                     EMVAINDTFGESGTPEELMVKYGLDAPSIVKAAKRAIARKNN"
     gene            1699..>2245
                     /locus_tag="PLX53_05905"
     CDS             1699..>2245
                     /locus_tag="PLX53_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOY72653.1"
                     /translation="MECYSDKELLEMFRNGETRNFAFNLIVNKYQERLYWHIRKIVVG
                     HDDADDVIQNTFIKVWGGLETFREESQLFTWLYRIATNEALTFLKKKKVKSFLSMDDV
                     ESQLAKTLETDAYIDGNELQLKLQKAILKLPEKQRLVFNMKYFEEMKYEEMSEILGTS
                     VGALKASFHHAVKKIEKFMGEN"
BASE COUNT          667 a          512 c          539 g          527 t
ORIGIN      
        1 aaaaccctac acgaagccgt ggagcacacc ggtaagggta aaccggtgat aattctcatg
       61 cgaacccaga tgggcatggg tgtcgatttt atggtgggta atcataagtg gcatggaaaa
      121 gccccaaacg atgaggagtt tgcaaaggcc atggcacaac tgcccgaaac aataggcgac
      181 ttttaaacta tttaaataat ttatacaatg aagaacattg cgctaattgc ccatgatgcc
      241 aagaaacccg agttggccag gtttctcaaa gaccatgaag agtggctgcc gggagttaac
      301 ctgttggcca cagggagaac tgctgaattt cttgaggagc aggggataaa atgccgccac
      361 cttagtcctg gccaatcggg tggttacaac gagataaccg gcatgattca acgcggggaa
      421 gttgatatag ttatctttct gcgcgatccc aaggtggtgc agcagcacca tgaggatatt
      481 cgcttactgc tagaggcctg caacatcaaa aatatccctt tggcaacaaa taaggcaacc
      541 gcagagctgg ttattcttgg gcttatccac atggaggcca ttgaacgtat gaagcgaaac
      601 aaataaccac cgaccattct cgtttgccaa tttggtaatc tgctgttggc tttcctgaac
      661 tcctaacaga actaccttaa accaacagca attttcaaca gattttttgt tttaaaaaaa
      721 tacatttaca acggatgaag tacacttcaa caggaaacaa ggatacacgt tcgggctttg
      781 gagcaggtct atatgaactg ggtaaagcta accctaatgt cgtagccctt tgcgccgatc
      841 tgataggctc gctcaagatg gaagactttt ccagagaatt tcccgacaga ttttttcaaa
      901 ccggcatagc cgaagccaac atgattggac tttctgcagg tcttaccatt ggtggtaaaa
      961 ttccctttgc tgggaccttt gcagtttttg ccaccggaag ggtttacgac cagattaggc
     1021 aaagcgtcgc ctactcgaat aaaaatgtga agatttgcgc ttcacatgca ggaatcacct
     1081 tgggtgaaga tggggctacc catcaaatgc tcgaggatat tgggcttatg aaaatgttgc
     1141 ccaatatgac tgttatcaat acctgcgact acaaccaaac caaagcagct acaatagcca
     1201 tagcaaacca cgttggaccg gtttaccttc ggtttggacg tccggcagta cctaacttca
     1261 cccctgccga tcagcaattt accattggaa aagcggtgaa gttaaccgaa ggaactcagg
     1321 tgtctgtttt cgccactgga cacctggtat ggaaagccct tgaggcagcc gacatgctgg
     1381 aacagcaagg aatttcggcc gaagtggtta acatccacac cattaaaccc ctggatgagg
     1441 aggcgattat cagatcaatt cagaaaaccg gttgtgccgt gaccgccgag gagcacatga
     1501 tgaatggtgg cttgggcgac agcattgccc aggtggttgc ccgtattcat cccgtacccc
     1561 tcgaaatggt tgccattaat gacacgtttg gcgaaagcgg aacccccgag gagctaatgg
     1621 tgaagtacgg ccttgacgca cccagtattg ttaaagcagc aaagagggct atcgcccgaa
     1681 aaaataacta atggtgaaat ggagtgctat tccgataagg agttgctgga aatgttccgc
     1741 aacggggaaa cccggaactt cgcctttaac cttattgtga ataagtacca ggaaaggctc
     1801 tactggcata tccgaaagat tgttgttggc cacgacgatg ccgacgacgt tatccagaac
     1861 acctttatca aggtttgggg tggcctcgaa accttcaggg aggaatcgca gctcttcacc
     1921 tggctttacc ggattgccac aaacgaagcg cttacctttc taaagaaaaa aaaggtaaag
     1981 tcattcctat ctatggacga cgtagaatcg caacttgcca aaaccctcga aaccgacgcc
     2041 tacatagatg gtaacgagct acagctaaaa ctacaaaagg ccatacttaa actacccgag
     2101 aaacaacggc tggttttcaa catgaagtac tttgaagaga tgaagtacga ggaaatgtcc
     2161 gaaattcttg gaacctcggt tggggcgctt aaagcttcat tccatcatgc agtgaaaaag
     2221 attgaaaaat tcatggggga gaatt
//
LOCUS       DAOOQX010000326         2235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_59846_length_2235_cov_3.703211, whole genome shotgun sequence.
ACCESSION   DAOOQX010000326 DAOOQX010000000
VERSION     DAOOQX010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59846_length_2235_cov_3.703211"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /gene="porQ"
                     /locus_tag="PLX53_05910"
     CDS             <1..1038
                     /gene="porQ"
                     /locus_tag="PLX53_05910"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOY72654.1"
                     /translation="CFITVLVLLSSLCGFSQVGGFGAYQFLNLPFSVRSAALGGKVAT
                     IDEPDMAMVMLNPALLDSTLHNNLYLSYSRYVDDISYGAFAYGRSSRWGTFGVNIQQV
                     SYGSFIEADETGLVQGTFKANELAVSLMYARTLDSLFSVGISVKPVYSHLERYTSFGL
                     AVDIGGNYHSRNGLFSSGVVLKNIGLMLKGYTRGNPETLPFEIVAGVSQKLEHAPFRF
                     LVTFHQLQNMNIYYESKLQDSETSLGENSSQPGFAERAGREFLSHLIIGMEFVPVKSF
                     YLRAGYNYQRRNELKVPEKVSMVGFSWGFGVKVAKLHISYSRATYHLAGSTNQFSICT
                     NLNNFVSTKPI"
     gene            1139..1828
                     /gene="cmk"
                     /locus_tag="PLX53_05915"
     CDS             1139..1828
                     /gene="cmk"
                     /locus_tag="PLX53_05915"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOY72655.1"
                     /translation="MGKKITIAIDGFSSCGKSTFAKKIAERLGYLYIDSGAMYRAVTL
                     EFLNWEAFRNNTPDTSMTQQILSGLQVGFSFNEQKKTYETTLNGKVVEDAIRSPRISE
                     LVSAVSAIPEVREKMVELQRLMGKDKGVVMDGRDIGTVVFPDAELKIFMTADPAIRAE
                     RRYKELQSKGVQASLEEVLNNILERDKLDQTRAISPLRKAEDAIELDNSYMTIDEQME
                     WAEKLIAARIC"
     gene            1966..>2235
                     /locus_tag="PLX53_05920"
     CDS             1966..>2235
                     /locus_tag="PLX53_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOY72656.1"
                     /translation="MQVDIDQKSGFCFGVVNAINQAEVGLKQNEEFYCLGSIVHNEQE
                     ISRLEKKGLRVVSRDEFSGLSGKKVLVRAHGEPPSTYELAKENDVE"
BASE COUNT          630 a          495 c          564 g          546 t
ORIGIN      
        1 tgtttcataa cagtattggt tttgctcagc agtctttgtg ggtttagcca ggttggagga
       61 tttggagcct accagttctt gaaccttcca ttctcggttc gttctgctgc actgggaggc
      121 aaggtggcaa ctattgacga accggacatg gccatggtga tgcttaaccc tgccctgctg
      181 gattcaacgc ttcacaataa cctttacctt tcctactcac gctacgttga cgatataagc
      241 tatggtgcat ttgcctatgg caggagcagc agatggggga catttggggt taacatccag
      301 caggttagct atggatcatt catagaggcc gatgaaaccg gattggttca gggaacattt
      361 aaggccaacg agttagccgt gtcgctaatg tatgctagga cactcgatag ccttttcagt
      421 gttggcatca gcgtaaaacc ggtatattcg catctggagc gttacacctc atttgggctt
      481 gcagtggata ttggtggcaa ctaccactcg cgtaacgggc tgttttcgtc gggagtagtg
      541 ttgaaaaata ttgggctaat gcttaagggt tatacccggg gtaacccgga aaccttgcct
      601 tttgaaattg tagccggagt aagccaaaag ctggagcatg cccccttccg ttttctggtt
      661 acattccatc agctgcaaaa catgaacatc tactacgaat caaagctgca ggatagtgaa
      721 acaagcctgg gcgaaaatag ctcacaacca ggatttgccg aaagggcagg aagggaattt
      781 cttagccatt tgataatcgg aatggagttt gttccggtta aaagctttta cctaagggcg
      841 ggatacaact accagcgccg aaatgagctg aaggtgcccg agaaagtatc catggttggc
      901 ttcagctggg gatttggagt aaaagttgca aaacttcata tcagctacag ccgtgccacc
      961 taccatttgg caggatcaac caaccagttc tcaatatgca ccaacctcaa caattttgtt
     1021 agcacaaagc caatttagcc gaatagactt aactcttttt ttataataac tttgctccgg
     1081 attttccagc agtcctaggc tgcttccgca acacatctat actaacgcga taatctggat
     1141 gggtaaaaaa ataacaattg ccattgatgg cttctcgtcg tgcgggaaaa gcacctttgc
     1201 aaaaaaaatt gccgagcgtt tgggatacct ctacattgac agtggagcaa tgtaccgggc
     1261 agtaacccta gaatttctta attgggaagc attccgcaac aacacacccg acaccagcat
     1321 gacccaacaa atcctgtcgg gattacaggt gggatttagc ttcaacgagc agaaaaaaac
     1381 ttacgaaacc accctaaacg gaaaggtggt ggaggatgcc attcgcagcc cacgcatttc
     1441 ggagttggtt agcgccgtat cggccattcc cgaggtaagg gaaaagatgg ttgagctgca
     1501 acgccttatg ggcaaggata aaggcgttgt aatggatggt cgtgatatcg gaaccgtggt
     1561 gtttcccgat gccgagttga agatttttat gaccgccgat cctgcaatca gggcagaacg
     1621 tcggtataaa gagctgcaat caaagggagt acaggccagc ttggaggagg tgcttaataa
     1681 catattggaa agagacaagc ttgaccaaac ccgcgccatt tctccactcc ggaaggctga
     1741 agacgccata gaacttgaca atagctacat gaccatagac gagcagatgg agtgggccga
     1801 aaagttgatt gccgcaagaa tctgctgaac caaactcccg aaaggttgtt acatatttgg
     1861 tatgaggttg acggttgccg gcaagcaaaa agaatgttca ctatgcgcaa accggattta
     1921 aatccataag cgctacttta aaatcagcat tgaattggta cctccatgca agttgatata
     1981 gatcagaaat cgggattttg ctttggcgtt gtaaacgcca taaaccaagc tgaagtcggg
     2041 cttaagcaga atgaggagtt ttactgcctg ggcagcatcg ttcacaacga gcaggaaatt
     2101 tcacggctcg aaaaaaaagg gctaagggtt gtatcccgag acgagttctc cggcctttcg
     2161 ggtaaaaagg tgctggtgcg tgcccacggc gaaccaccgt caacctacga gctggcaaaa
     2221 gaaaacgacg ttgag
//
LOCUS       DAOOQX010000327         2231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_60026_length_2231_cov_3.154871, whole genome shotgun sequence.
ACCESSION   DAOOQX010000327 DAOOQX010000000
VERSION     DAOOQX010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60026_length_2231_cov_3.154871"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..684
                     /locus_tag="PLX53_05925"
     CDS             172..684
                     /locus_tag="PLX53_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72657.1"
                     /translation="MALNIETKKATGSGLVKIFLLHFFIALLAASFFLFVILLVGREL
                     SWKDFFSYFIIFIPITVVLPLILGIAAASSYKRVEIILTPASSVNLAKLKEFFLKSNY
                     LPAAEESSKFTFKRSNTFRRALCVSSDMPKIQVKGQAVGITMLKRKSATLINQLRNDR
                     RYEVGSEKVE"
     gene            684..1787
                     /locus_tag="PLX53_05930"
     CDS             684..1787
                     /locus_tag="PLX53_05930"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOY72658.1"
                     /translation="MSSGPFILLDSARCRQNIARMEEKARTNGLVFRPHFKTHQSHAV
                     GRWFREVGVNRITVSSAEMAGYFAKDGWNDITIAFPLSRFHVEAVNRLAEDIQLNITF
                     SSIRNLLDALPLISQNVGVLIEVDVGHHRTGVNISDTRQIALMVNQVAENPKLAFRGF
                     IAHAGQSYSLTGEKNVLAIHNQAMGELVKLKSFWSQQYPEIVISYGDTPTCSLADDFW
                     GVNEIRPGNFVFYDIMQHLIGSCSLDDIALALICPVVDVYPEREEAVVHCGAVHLSKD
                     FVLFPESNRCYGLVCPYNGIRWGAPVEGAYVTSLSQEHGIISFGASGQPFPFHVGDLV
                     AILPVHSCLTLDTMGEMVTESGEKISTMRSRKR"
BASE COUNT          629 a          448 c          482 g          672 t
ORIGIN      
        1 tttggaggat ggatttttaa taattgataa ttgaatgaaa caccttttta ggataacttg
       61 tataaacaca catgaaaacg aattgggtgt tccatacgct tagctcagat gcctagaaga
      121 tgtgggccaa aggattgtaa tcagtaacct ataaaatttt aaacagatat tatggcgctt
      181 aatatagaaa ccaaaaaagc aacaggctca ggtcttgtta aaattttcct acttcacttt
      241 tttattgcgc tgctagctgc ttcttttttc ctttttgtta ttcttttagt gggcagggag
      301 cttagctgga aagatttttt tagctacttc ataatcttta ttccaattac tgttgtgctt
      361 ccgttgattc tgggaattgc agctgcatct tcatacaaac gggtcgaaat catacttact
      421 ccagcatcaa gcgtaaatct tgcaaagctg aaggaattct tcctcaagag taactacctg
      481 ccagctgctg aggaaagttc aaagtttacc ttcaaacgct ctaatacttt taggagagcc
      541 ttatgcgtta gtagcgatat gcctaaaatt caggtaaagg gacaggctgt tggcattact
      601 atgcttaagc gaaaatcagc taccctgata aatcagctta gaaatgatag gcgctatgag
      661 gttggttctg aaaaagttga atgatgagta gcggtccatt catacttctg gatagtgccc
      721 gatgcaggca aaatattgcc cgcatggaag aaaaggctag gactaatggt ctggtattta
      781 ggccacattt taaaactcat cagagccacg ctgttggccg ctggtttcgc gaggtagggg
      841 ttaacagaat taccgtttca tcggctgaaa tggccggata ttttgccaag gacggatgga
      901 acgatattac catagcattt cctctatccc gtttccatgt agaagctgta aatcgtcttg
      961 ctgaggatat acagctgaat atcacctttt catcaattcg taatttgctg gatgcgttac
     1021 ccctgattag ccaaaatgtg ggtgtactca tcgaggttga tgttggccat catcgtacgg
     1081 gtgttaatat ttcggatact cgacagatcg ctctaatggt gaatcaagtt gcggagaacc
     1141 ctaagctggc ttttcgcgga tttattgccc atgctggcca gtcctacagc ctaacaggtg
     1201 aaaaaaatgt tttagctatt cataaccagg ccatgggcga gctggttaag ctaaagtcgt
     1261 tctggagcca gcagtaccct gaaattgtca tatcatacgg cgacacaccc acctgttcgt
     1321 tggccgatga tttctggggt gtcaacgaaa ttcgccccgg taacttcgtt ttttacgata
     1381 ttatgcagca cttgattggc tcgtgcagcc tggatgacat tgccctggcc ttgatttgtc
     1441 cggtggttga tgtttatccc gaaagagagg aggcagttgt ccactgtgga gccgtacact
     1501 tgtcgaaaga tttcgttctt ttcccggaaa gcaacaggtg ctacggcctg gtttgtccct
     1561 ataatggtat aaggtgggga gcgccagtag agggagccta tgtaacctcc ctgagccagg
     1621 aacacgggat catctccttt ggtgcgtcgg gacaaccttt tccttttcat gtaggtgatt
     1681 tggttgccat tctacctgtc cacagctgcc taacgctcga taccatgggt gaaatggtta
     1741 ccgaaagcgg tgaaaaaatt tcaaccatga gaagcagaaa aagatgaagt acataaccaa
     1801 atatcaagac tagtaaaagt tgaaactttt gtagcctgct atcactggcg acaagaacac
     1861 atgactgtta aggtaatatg gatctactaa agaacagtaa aacgtaacgc aagcctattc
     1921 caaaactatt tgatatcaaa atagctgcta ccaaggggtt ctactgagtt tagctaatgt
     1981 tctacagcgc tagtattttt tcttaaccgt tttcaatttt attcaagttt ctatgtactt
     2041 tgcagtactt tcgcaagcac gcacaaaccc attcccaaac cacttgcttg caaaagaact
     2101 gcaatttagc ctaagcactc ctacatatta acattttgta aagggcttaa tatttattta
     2161 tctttaaaaa gcgtttatgt tttttaaaac tcaatcagat ggatagtgat agtaataaaa
     2221 ttggactctt t
//
LOCUS       DAOOQX010000328         2221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_60430_length_2221_cov_3.734072, whole genome shotgun sequence.
ACCESSION   DAOOQX010000328 DAOOQX010000000
VERSION     DAOOQX010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60430_length_2221_cov_3.734072"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            603..1622
                     /locus_tag="PLX53_05935"
     CDS             603..1622
                     /locus_tag="PLX53_05935"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOY72659.1"
                     /translation="MQNLDWKNLPFGYLKTDYNVRCRYSDGKWGNLEVSDSEYINIHM
                     SATALHYGQEGFEGMKAYRGKDGRIRIFRWEENAKRLQLTADGILMAPVPMELFHEAV
                     VKAVKLNEKYVPPYGTGASLYIRPLLFGSGAEIGVKPAKEYVFIVFVTPVGPYFKEGF
                     NPTKIAIIRDSDRAAPLGTGTFKIGGNYAASLRGTIKAHNAGYSSPMYLDACGKRYID
                     EIGAANFFAIKNNTYITPKSTSILNSITNMSVMQLAQDLGLKVERRPVAVEELETFEE
                     VGACGTAAVISPIGEINDIDGGKVYKFCKDGKAGPISTKLYQTLTGIQYGDIEDKHGW
                     VSFVE"
     gene            complement(1740..>2221)
                     /locus_tag="PLX53_05940"
     CDS             complement(1740..>2221)
                     /locus_tag="PLX53_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY72660.1"
                     /translation="PLYMGTLYVDVENLALLAADFELNPKYIEKAASSLIVRKNRSVK
                     VKPQQVSYSVSYRELNGVYYVQHIRGDISVRISSRYRLFTDSYNLFFEFVNMQIDTLN
                     VQRFSRRDVVKPYTVFSDGDFAYDASFWGDMNFIEPEEDVARAIARINPKIESVVGE"
BASE COUNT          624 a          505 c          509 g          583 t
ORIGIN      
        1 cccaatccat accgcccaac cagggcgctc aacattctcc ttactaaatt taaaacccat
       61 agtccttccg gatttgcttt ttaatagctg ctacagcgtt ctttcattct ctccactaaa
      121 acttacgcac tactcaacac cataggggat gacaaatttc gttgggctct ccgccggctg
      181 gcgaactggt tcgtgtggta gcatggggtg tggtagccta ttgttttgct caacgtggtt
      241 tccttcattg ttgggccagc catgaaggcc gtcccctcca tggtggtctc aagagtaaac
      301 actaaaatgc gccacgctcg tataaaaaaa tcatcgccac tacgaggcgt tttaaaaaat
      361 ccttacgagt attgccaacc cggtcaatct ccgccataca gataaaaact agcatcagct
      421 caaaatgtcg tatgcactgt taccatcacc gtgtagttac aatttttaat tcatattgaa
      481 tattttatta aacttttaaa tcatttagct gtcataatag cactaaatgt taggagaaat
      541 aatataattc tactaatttt gtgaggcaac attgttaata acaattctaa aaatctgcag
      601 tcatgcaaaa tcttgattgg aaaaaccttc cgtttggtta tttgaaaacc gattacaacg
      661 taaggtgtcg ttatagcgac ggtaaatggg gtaatctgga ggtttcggat tcggagtata
      721 tcaacattca catgtcggct accgccctgc actacgggca ggaaggcttt gagggcatga
      781 aggcctatcg tggtaaagac ggtcggattc gcattttccg ttgggaggaa aacgccaagc
      841 ggcttcagct aacagctgat ggcatactga tggcacccgt gccgatggag ctttttcacg
      901 aggcggtagt aaaagccgtt aagctaaacg agaagtatgt tccaccctac ggaacaggag
      961 catcgctata catcaggccg ctgctgttcg ggtcgggtgc cgaaattggc gttaaaccgg
     1021 caaaggagta cgtttttatt gtattcgtta cgcccgtagg cccctacttc aaggagggct
     1081 ttaacccaac aaaaatagcc attatacgtg atagcgatag agccgctccg ctgggaaccg
     1141 gcacatttaa aattggtggt aactatgcag ccagccttcg tggcaccata aaagcgcaca
     1201 atgcaggcta cagctcccca atgtaccttg atgcctgcgg aaagaggtat atagatgaaa
     1261 ttggtgctgc aaacttcttc gccatcaaga acaacactta cattactcca aaatcaacct
     1321 ctatactcaa ctccattacc aacatgagcg taatgcagct ggcacaggat ttaggcctga
     1381 aggttgagcg ccgtccggtt gccgttgagg aacttgagac ttttgaggag gtcggagcat
     1441 gcggcactgc tgcagttatt tcgccaattg gcgaaataaa cgatattgac ggcggaaagg
     1501 tttataaatt ctgtaaagat ggaaaggcag ggccaatatc aaccaagcta taccagaccc
     1561 ttacaggaat tcagtacggt gatattgaag ataagcatgg atgggttagc tttgttgaat
     1621 aagtagtatt aaagtaacgc acaaaaaccc ttgggggtct tctcccgagg gttttttatc
     1681 gggttatacc tttatagggt taaaatacca tgcaggtaat tgcaaaccga agaggctagc
     1741 tactccccta cgacactctc aattttaggg ttgattcggg caattgcccg ggccacatcc
     1801 tcctcgggct caatgaagtt catatctccc caaaaactcg catcgtaggc aaaatcccca
     1861 tcggagaaaa cggtgtaggg ttttacaaca tctcgtcttg aaaagcgctg aacgttaagg
     1921 gtgtcaatct gcatgttcac gaattcgaaa aacaggttgt agctgtcggt aaatagcctg
     1981 tagcgtgagc taatgcgtac ggaaatatca ccccggatat gctgcacgta gtaaactccg
     2041 ttaagctccc tgtaggacac agagtagcta acctgctggg gttttacctt tacggagcga
     2101 tttttcctaa caataaggct gctggctgcc ttttcaatat acttcgggtt tagctcgaag
     2161 tcggcagcca ggagcgcaag gttttccaca tcaacgtaaa gggttcccat gtaaagaggg
     2221 a
//
LOCUS       DAOOQX010000329         2218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_60555_length_2218_cov_2.641239, whole genome shotgun sequence.
ACCESSION   DAOOQX010000329 DAOOQX010000000
VERSION     DAOOQX010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60555_length_2218_cov_2.641239"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /gene="rplE"
                     /locus_tag="PLX53_05945"
     CDS             complement(<1..120)
                     /gene="rplE"
                     /locus_tag="PLX53_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853986.1"
                     /note="part of 50S and 5S/L5/L18/L25 subcomplex; contacts
                     5S rRNA and P site tRNA; forms a bridge to the 30S subunit
                     in the ribosome by binding to S13; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOY72661.1"
                     /translation="MEYVANFKRKYKEEIVPALMKEFGYKSVMQVPRLEKIVIN"
     gene            complement(120..443)
                     /gene="rplX"
                     /locus_tag="PLX53_05950"
     CDS             complement(120..443)
                     /gene="rplX"
                     /locus_tag="PLX53_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOY72662.1"
                     /translation="MASKLHIKKGDLVYVISGESKGKQGRVLSVQVSKQRAFVEGVNM
                     VSKHTKPNSKYPQGGIIKMEAPIHISNLMLIDPASGKPTRIGRRLNDKGKLVRYSKKS
                     GEEIK"
     gene            complement(466..831)
                     /gene="rplN"
                     /locus_tag="PLX53_05955"
     CDS             complement(466..831)
                     /gene="rplN"
                     /locus_tag="PLX53_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOY72663.1"
                     /translation="MIQQETRLKVADNSGAKEVLCIRVLGGTGMRYARIGDVIVVTVK
                     NAIPSGEIKKGTVSKAVVVRTKKEIRRPDGSYIRFDDNACVLLNAQGEIRGTRIFGPV
                     ARELRDNYMKIVSLAPEVL"
     gene            complement(834..1097)
                     /gene="rpsQ"
                     /locus_tag="PLX53_05960"
     CDS             complement(834..1097)
                     /gene="rpsQ"
                     /locus_tag="PLX53_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOY72664.1"
                     /translation="MEEVKRNLRKVRTGVVVSNKMDKSIVVAVHRKVKHPTYGKYVKK
                     TTKFYAHDEANTCNIGDVVKIMETRPLSKLKRWRLVEIIERAK"
     gene            complement(1110..1310)
                     /gene="rpmC"
                     /locus_tag="PLX53_05965"
     CDS             complement(1110..1310)
                     /gene="rpmC"
                     /locus_tag="PLX53_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOY72665.1"
                     /translation="MKASEVRELTDKEIVERIEAEKTNLLKLKLNHSISPLDNPMKIK
                     ESRRTIARLKTILTQRKLSQNQ"
     gene            complement(1318..1746)
                     /gene="rplP"
                     /locus_tag="PLX53_05970"
     CDS             complement(1318..1746)
                     /gene="rplP"
                     /locus_tag="PLX53_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOY72666.1"
                     /translation="MLQPKKTKYRRQQKGRMKGNAQRGNQLAFGSFGIKALDSAWITS
                     RQIEAARQALTRHMKREGQIWIRIFPDKPITKKPAEVRMGKGKGAPEGFVAVVTPGRM
                     LFECEGVSLDIAKEALRLAAQKLPIATKFVVRNDYVEPVS"
     gene            complement(1769..>2218)
                     /gene="rpsC"
                     /locus_tag="PLX53_05975"
     CDS             complement(1769..>2218)
                     /gene="rpsC"
                     /locus_tag="PLX53_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOY72667.1"
                     /translation="NKEVQINIFEVKRPDLDAVIVSNNIARQLEGRISFRRAVKTAIA
                     STMRMGAEGIKVQVSGRLGGAEMARSETYKEGRIPLHTFRADIDYALAEAHTKVGLIG
                     IKVWICNGLVYGKRDLSPNLGISSAAPAQSGHGQARPHGARKKKSNK"
BASE COUNT          487 a          560 c          468 g          703 t
ORIGIN      
        1 gttgatgaca atcttttcca gccggggaac ctgcatcacg cttttgtatc caaactcctt
       61 catcaaagcg ggaacaattt cctccttata cttccttttg aaattggcaa cgtattccat
      121 tacttaatct cctctcctga tttttttgag tatctaacca gctttccctt atcgttaagc
      181 ctgcggccaa tgcgggtagg cttacctgat gcaggatcaa tcagcatcag gttggatata
      241 tgaattgggg cttccatctt gataattcca ccctgagggt acttggagtt gggcttggta
      301 tgcttggaca ccatgttaac cccttcaaca aaggcgcgct gtttgcttac ctgaacgcta
      361 agcactctgc cctgcttgcc cttgctttcc cctgaaatga cgtaaacaag gtcccctttc
      421 tttatatgta gcttgcttgc catatgtaat ttgaatttta agggattaca gcacctcggg
      481 tgctagtgat acaattttca tgtagttatc ccggagctcc ctggccacgg gaccaaatat
      541 gcgggttccg cgaatttcgc cttgtgcatt aagcagcaca caggcgttat catcgaaacg
      601 gatataggag ccgtcgggcc tgcgtatttc ctttttagtt ctaacgacta ctgcttttga
      661 aacggtaccc ttcttaattt cgccagaagg aatggcattt ttcacggtaa cgacaatcac
      721 atcgccaatc cgggcatagc gcatgccggt accaccgaga acgcgaatgc acaaaacctc
      781 tttggctcca ctgttatcgg ctacttttaa tcgtgtttcc tgctgtatca tggctatttc
      841 gctctttcaa tgatttctac taatctccag cgtttcagct tgctgagagg acgggtttcc
      901 attatcttca ccacatcgcc tatgttgcag gtatttgcct catcatgggc gtaaaatttg
      961 gtggtttttt tcacgtactt tccgtatgta ggatgtttta ctttgcggtg aacagcaact
     1021 acaatagact tgtccatctt attgctaacc acaacacctg ttcgtacctt tcgtaaattt
     1081 cgcttaactt cttccattgc aattcaacat tactggttct gacttagctt ccgttgggtt
     1141 aagattgttt tcaaccgggc aatagtacga cgcgattcct taatcttcat ggggttgtcc
     1201 agcggtgaaa tgctatggtt taacttgagc ttaaggaggt tggtcttttc tgcttcgatt
     1261 cgttcaacaa tctctttatc ggtaagttct cttacttctg atgctttcat gttctagcta
     1321 gcttacaggt tcaacataat cattccgtac tacgaattta gtagcaatgg gaagtttttg
     1381 agcggccagg cggagagctt ccttggcaat atcaagcgaa accccctcac actcgaaaag
     1441 catgcggccg ggggttacca cagccacaaa accctcaggt gctccttttc cctttcccat
     1501 tcgaacttca gccggttttt ttgttatggg tttatcgggg aatatgcgaa tccatatttg
     1561 tccctcacgc ttcatgtgac gggtaagagc ctgacgggca gcttcaatct gtcggctggt
     1621 aatccaagcc gaatccaaag ccttaatccc gaatgaaccg aatgccagct ggtttccccg
     1681 ttgggcattg cccttcattc tacccttttg ctgccttcta tacttggtct tttttggttg
     1741 taacatttcc agaatgcttt aaggtgttct acttattact tttcttttta cgagcaccat
     1801 gaggcctggc ttgtccatgc cccgattggg caggtgcggc agatgaaata ccaaggtttg
     1861 gcgagaggtc tcttttaccg taaaccagcc cgttgcatat ccacaccttg ataccaatta
     1921 agcctacctt ggtgtgggct tcggccaacg cgtaatctat gtcggcacga aaggtatgta
     1981 ggggtattct tccctccttg taggtttcgg aacgggccat ttcagctcca cccaaccgac
     2041 ccgatacttg aacctttatc ccctcagctc ccattctcat ggttgatgct atagcggttt
     2101 taacggctct gcggaaggaa attcttccct cgagctgccg tgcaatattg ttgctaacta
     2161 tcactgcatc caggtcgggg cgttttacct caaatatgtt gatctgcacc tccttgtt
//
LOCUS       DAOOQX010000330         2217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_60576_length_2217_cov_4.084181, whole genome shotgun sequence.
ACCESSION   DAOOQX010000330 DAOOQX010000000
VERSION     DAOOQX010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60576_length_2217_cov_4.084181"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1692)
                     /locus_tag="PLX53_05980"
     CDS             complement(<1..1692)
                     /locus_tag="PLX53_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72668.1"
                     /translation="MRKLMLLSVLLVVSLGLQSQELVRYQVPPAEMVAILEAPPTPGF
                     LLSPDGKHALLSYPQVLPDLAELAKPELKLAGFRVNPENFGRYDSRIFDRLELLNISN
                     KMVTSLSGIPDSGHITSFSWSPDGKNVAISVYFNSSVELWIVEVESGVARPLSASLNE
                     SVISPGFAWHPDGKSLYVLKHREGMKKPGREKLPLGPSVQQATGQKSQVRTFQDLLKS
                     PADEELFEYYATSQLYRVNLLGEASEVGNPGIISQVEPSPDGSYIYVEQVVRPFSYIV
                     PAQYFPRLCHIWNADGHLVKRLAEQPLADDIPSGFSAARKGKREMQWRSDVPSTIVWV
                     EAQDDGDPHRDVKIRDVVYSLDAPFRGEPVKLVELETRFSSISWGWSNLAVAYGRWWS
                     SRRQTASLFNPSAPTDRPRVLWDRSWEDAYTDPGQLVMVNNANGFSVLQTNSSRTKLY
                     LSGKGSSPEGDMPFLDEINLKNLSVKRLWQCHAPLYEYFIGFYDMDNSRIVTSQESVN
                     RQPNFFIRDIKRQKLVQLTNFPDPYPGLHGVHKELVTYRRPDGVTLTGTLYLQARI"
     gene            1911..>2217
                     /locus_tag="PLX53_05985"
     CDS             1911..>2217
                     /locus_tag="PLX53_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOY72669.1"
                     /translation="MVNLQEIIDAAWENREMLGTPEVNDAIKSVVEMLDKGEVRVAEP
                     ADDGWKVNEWVKKAVLLYFSICSFQATGAGPLEFYDKIPLKKNYQSLGVRVVPHAVAR
                     "
BASE COUNT          588 a          539 c          522 g          568 t
ORIGIN      
        1 tatcctggct tgcaggtaga gtgtcccggt cagggtaacc ccatcgggac gtcggtaggt
       61 aaccagctcc ttgtgcactc catgcaagcc aggatatggg tcagggaaat tggtaagctg
      121 taccagcttc tgcctcttga tatcacggat gaagaagttg ggctgcctgt taaccgattc
      181 ctgggaggta acaatacgcg agttatccat atcgtaaaaa ccgataaagt actcgtagag
      241 cggggcatgg cattgccaca gccttttcac gcttaggttt tttaagttta tttcgtctaa
      301 aaagggcata tcgccctcgg gcgaagagcc tttaccgcta agatagagtt ttgtcctgct
      361 gctgttggtt tgtagcacgc tgaacccatt agcgttattt accattacaa gttgtcccgg
      421 atcggtgtag gcatcctccc acgacctatc ccaaagtacc cttggcctat cggttggtgc
      481 cgaaggatta aatagggaag cggtttgcct tcggctactc caccaacggc cgtaagcaac
      541 cgcaaggtta ctccatcccc aactaattga ggagaaacgt gtttcaagtt caaccaattt
      601 taccggttcg cctctaaacg gggcatcaag cgagtaaact acatcgcgaa ttttaacatc
      661 ccggtgtgga tcgccgtcgt cctgagcctc cacccacaca atggttgaag gaacatccga
      721 acgccactgc atctcccttt tccccttacg agcagcgctg aatcccgatg gtatgtcatc
      781 ggcaagaggt tgttcggcta atcgctttac caaatgccca tccgcgttcc atatatggca
      841 gagccttgga aaatattgcg ccggaacaat gtatgaaaat ggccttacaa cctgctccac
      901 gtagatatag ctaccatcgg gcgatggctc aacttgtgaa attatgcccg ggttacccac
      961 ctcggaagcc tctccaagta aatttaccct gtaaagctgc gaagtggcgt agtactcaaa
     1021 caactcctcg tcggcaggtg atttgagcaa gtcctgaaat gtcctcacct gagacttctg
     1081 tcccgtagct tgctgcaccg atggacccaa gggcaacttt tccctgccgg gctttttcat
     1141 accttcacgg tgcttcaata cgtaaagcga tttgccatcg ggatgccacg caaatccagg
     1201 agaaataaca gactcgttca aactagctga tagcggcctg gctactccac tttcaacctc
     1261 aactatccaa agttcaaccg agctgttgaa ataaacactt atggcaacat ttttcccgtc
     1321 aggggaccag ctgaaagagg taatatgacc gctatcgggt atccccgaaa gagaagtaac
     1381 cattttgtta ctaatattga gcaactccaa cctgtcgaat attcgggaat cgtaacgtcc
     1441 aaagttttcg gggttaaccc tgaagcctgc cagcttcagc tcgggcttag ccagctcggc
     1501 caggtcgggc agaacttgag ggtatgacag caaggcatgt ttgccgtcgg ggctaagcaa
     1561 aaatcccgga gtgggaggtg cttccagaat ggccaccatt tctgccggcg gaacttggta
     1621 gcgaaccagc tcctgcgatt gcaagcctaa cgaaactacc aataaaaccg aaagtagcat
     1681 caattttctc atagccaagt aattttctat ttttaggtta taatcagccc ttatcaaaat
     1741 ctgcctgtaa aaaagctaca tttgcagctg aaccacgttt tggtttcagt ttaaacttta
     1801 tggattatat gcagcccgta taaggtttaa gctgaatgca aaaccttact aaaatactta
     1861 ctttttgact tctcaatata gaattggttg aacagaaata ctgacaattt atggttaatc
     1921 ttcaggaaat tatagatgca gcatgggaaa atcgcgaaat gctaggtacc cctgaggtaa
     1981 acgatgctat taaaagcgtg gttgaaatgc ttgacaaggg agaagttcgt gtggccgagc
     2041 ctgctgatga tgggtggaag gttaacgaat gggtgaagaa ggccgttttg ctctactttt
     2101 ccatatgtag ctttcaggct acaggggcag gcccgctgga gttttacgat aagatacccc
     2161 taaaaaagaa ctaccaatcg ctgggtgtaa gggtagttcc ccatgccgtg gcacggt
//
LOCUS       DAOOQX010000331         2211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_60845_length_2211_cov_3.311224, whole genome shotgun sequence.
ACCESSION   DAOOQX010000331 DAOOQX010000000
VERSION     DAOOQX010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60845_length_2211_cov_3.311224"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="PLX53_05990"
     CDS             complement(<1..1231)
                     /locus_tag="PLX53_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY72670.1"
                     /translation="MKRHLPILFLIILGSLGHSPLYGQLNKAYYYYKSEELISQGRYS
                     ESLPVLNTLIGIDSSLFEGWFLRGVAKYNLGDIRGAITDFDKSLKANPLSAQSYHYKA
                     IALSQVSEYDKALKEISSALELRPNSPDFLFTLGVLQFQTQNYPKAIGTFTRVIQLES
                     SNADAWYNRATAKLLIADTLGALSDYSTTINVNPFHAAAYARRGAVNAELDRNHLALA
                     DLNRAIELDSLKADFYFTRAIINYNFKKYQNSIDDLTKALRLNPNYQIALFNRAIINY
                     QAGNTDPAIDDLTRLMAINSQNVLVPYYRGSIYLEKGEATRSIDDFTKAISLFPDFAN
                     AYLLRSDAKRQLGNLPGAEEDFLKGQALANKFHGKGQAEITSLLDSSGKLNKLISLES
                     DFNLASTLSIESNRIRIA"
     gene            complement(1245..1991)
                     /gene="mtgA"
                     /locus_tag="PLX53_05995"
     CDS             complement(1245..1991)
                     /gene="mtgA"
                     /locus_tag="PLX53_05995"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOY72671.1"
                     /translation="MNGKNRQKSQRYQSFRRLVLYAIATFFITTVISVIAFSFINPNL
                     TMLMVAKRVQPLNDGGSNRINHKWVSIDKISPQLVLAVCAAEDNKFMEHNGFDWESIQ
                     KAKEHNKKGKRLRGASTITQQTVKNVFLWPRRSWVRKGFEAYFTFLVEVFWSKERIME
                     VYLNVAELGRGIYGVESASECYYGKPASKISTYEAAMLATVLPNPAKRNPAKPSSYMY
                     RYQRTILRNMYNLGRIDLKGDTSSKSKKKN"
BASE COUNT          548 a          461 c          516 g          686 t
ORIGIN      
        1 aggcaatgcg aattctgttt gattcgatgc ttaatgtaga agccaggtta aaatcgcttt
       61 caagcgaaat cagcttgttg agttttccgc tcgagtcgag caacgaagta atttcggcct
      121 gtccctttcc atggaattta ttagctagag cttggccttt caggaaatcc tcctcggctc
      181 ccggtaggtt acccagctgt ctttttgcat cggagcggag gaggtaggca ttggcgaagt
      241 cgggaaaaag gcttatggcc ttggtaaaat cgtcgataga ccgtgttgct tcaccctttt
      301 ccaggtatat agaacctctg tagtatggca ccagaacatt ttgggaattt attgccatta
      361 gccttgtaag gtcgtcaatt gctgggtctg tatttcctgc ctggtagtta ataattgccc
      421 tgttaaaaag cgctatttgg tagtttggat ttaatcgtag ggcttttgta aggtcatcaa
      481 tagagttttg atatttttta aagttatagt ttataatggc ccgggtaaag taaaagtcag
      541 ccttaaggct gtcgagctca atggctctgt ttaaatcggc taaagcaagg tggtttctat
      601 caagttcggc gtttacagct cccctgcggg cgtatgctgc agcatggaat gggttcacat
      661 taatggttgt gctgtagtcg cttaaagctc ccagagtatc ggctatgagc agtttggcag
      721 tggcgcggtt ataccatgcg tcggcattgg aactttcaag ctgaattacc cgtgtgaagg
      781 ttcctattgc cttagggtag ttctgggttt ggaattggag cacaccgagt gtgaagagaa
      841 aatcggggga gttaggtcta agctccagtg cggagctaat ctccttcaac gccttgtcat
      901 attctgatac ctgacttaag gcaattgctt tgtagtggta tgattgagca gatagcgggt
      961 tagcctttag cgacttgtcg aagtcggtga ttgcacccct tatgtcgccc aggttgtact
     1021 ttgccacccc tctaagaaac cagccctcga aaagggagga gtctattccg ataagggtgt
     1081 tcaaaaccgg tagcgattcg ctgtagcgcc cttgcgaaat caactcttcg cttttgtagt
     1141 agtagtaggc cttgtttagc tggccataaa gcggtgaatg gcctaaacta ccgagtatta
     1201 ttaaaaacag tataggcaga tgccgtttca atttactcac ttttttagtt tttctttttg
     1261 cttttactac ttgtatctcc tttaaggtct attcttccca ggttgtacat gtttcgaaga
     1321 atggtgcgct ggtacctgta catgtagctg ctaggcttag ccggattacg ctttgctggg
     1381 ttgggaagca cggtagccag catggcagcc tcataggtgc taatttttga agcgggtttt
     1441 ccatagtagc attccgaagc agactccact ccatatatgc ctcttcccaa ttcggctaca
     1501 ttgaggtaaa cctccataat tcgctccttg ctccaaaaaa cttctaccag aaatgtaaag
     1561 taggcctcaa atccctttct tacccatgaa cggcgcggcc atagaaatac attctttacg
     1621 gtttgctggg ttatggtgct agcgcctctt agccttttcc cctttttatt atgctccttg
     1681 gctttttgaa tggactccca gtcgaacccg ttgtgttcca taaacttatt atcctcagca
     1741 gcacacacag ccaaaactag ctggggagat attttatcaa tgcttaccca cttgtgatta
     1801 atccgattag atccgccgtc atttagcggc tgaactcgtt tggctaccat tagcatggta
     1861 aggtttggat tgataaaaga aaaagctatt accgatatga cggttgtaat aaagaatgtg
     1921 gcgatggcat ataatactaa ccgtcgaaac gattggtacc gctgtgattt ttgtctattc
     1981 ttaccattca ccgtcaaggc caaacgtgct tttttgcacg tgctaaatta taaatttata
     2041 aagtggattt caaaacagct ttaacggttg attatctcat tcaaccattt aaaaaaaata
     2101 gggttctgat tacaagtact ttagctcata acttttaagc atcggggagg aggggatgga
     2161 acacccaatg ttttggcttc aacaagctag caaaaggccc ggttgttcaa g
//
LOCUS       DAOOQX010000332         2211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_60849_length_2211_cov_2.898887, whole genome shotgun sequence.
ACCESSION   DAOOQX010000332 DAOOQX010000000
VERSION     DAOOQX010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60849_length_2211_cov_2.898887"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLX53_06000"
     CDS             complement(<1..226)
                     /locus_tag="PLX53_06000"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72672.1"
                     /translation="MIRFVIKRILYFILVVFGVVTLIFALFHMVPGDPARMVMGQRTD
                     SASLAAARADMGLDRPVAVQYLKYLNDLSPL"
     gene            complement(290..1099)
                     /gene="mazG"
                     /locus_tag="PLX53_06005"
     CDS             complement(290..1099)
                     /gene="mazG"
                     /locus_tag="PLX53_06005"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOY72673.1"
                     /translation="MDKKTDNITRNERAREAFDRLLTIMDELREKCPWDRSQTFETLR
                     PLTIEETYELSEEILDKNFQAIKKELGDILLHIVFYSKMAAEENQFDMAEVINSLCDK
                     LIYRHPHIYGNVEVKDANQVVENWEQLKITEKDGNKTVLSGVPASLPSLIKANRIQEK
                     VRAVGFDWEERGQVWDKVEEELEELKVEVERQNRNRIEGEFGDLLFSVVNAARLYNID
                     PETALERTNRKFMKRFGYLESKTIKQGQSLKDMTLDQMNAIWEESKQFDTE"
     gene            complement(1122..1967)
                     /locus_tag="PLX53_06010"
     CDS             complement(1122..1967)
                     /locus_tag="PLX53_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOY72674.1"
                     /translation="MLIFNIPDYSITFVMLLANPMDNLRIVTLTTDWNQNDYYVGILC
                     GRLLSIEPTLRIVELSHRVPPFDYIQAAFIMRGCFENFPPGTIHICMVNSDSTTSKQL
                     LLFELKGHFMIVPDNGIVGLITEVAPDRVYSIPFEEHSSFGSLSAVASAMNMLLFNSN
                     LADIALIAGTYKQNINIRAASDESSITGSVIYVDSYQNAITNITREQFEQVRKGRKFS
                     IYVQSFSYKIEEIVENYADVEDGDMLALFNSLNLLEIAIRNGFLTELLALSVGSSIMV
                     KFTNR"
BASE COUNT          583 a          525 c          507 g          596 t
ORIGIN      
        1 acaggggcga caggtcgttg aggtacttta ggtactgaac ggcaacgggc ctgtcgagcc
       61 ccatgtcagc acgtgccgct gccagcgagg ctgaatcggt gcgctgaccc atcaccatgc
      121 gggccggatc gccgggaacc atgtggaata gagcgaatat gagggtaacc accccaaaaa
      181 ccaccaggat gaagtaaagt attcttttaa taacgaagcg gatcattttg cgttgtaaat
      241 gaggagtgat taccttctgg ttgatgggca gcccaatgtt tattgctggt tactcggtat
      301 caaactgctt cgactcctcc caaatggcat tcatctggtc cagcgtcata tccttaagcg
      361 attgtccctg cttgatggtt ttagactcca ggtagccaaa ccgtttcatg aacttgcggt
      421 tggtgcgttc cagcgctgtt tcggggtcaa tattgtagag cctggccgca ttgacaactg
      481 aaaacaggag atcgccaaac tcgccctcaa ttcggttgcg gttttgcctt tccacttcta
      541 ccttcagctc ctcaagttcc tcctcaacct tatcccaaac ctgaccgcgc tcctcccagt
      601 cgaaaccaac ggctctaacc ttttcctgaa tcctgttggc cttaataagc gaggggagtg
      661 aggccggaac acccgaaagc acagttttgt tcccgtcctt ttcggtgatt ttcagctgct
      721 cccaattttc cactacctgg ttggcatcct ttacctcaac attaccgtag atatgcgggt
      781 gacggtaaat tagcttatcg caaagactgt ttataacctc agccatatca aactggttct
      841 cctcggcggc cattttggag taaaagacaa tgtgcagcag gatgtcgcca agctccttct
      901 tgattgcctg aaagttttta tccagaatct cctccgacaa ctcataggtt tcctcgatgg
      961 taagtggccg taaggtttcg aatgtttggc tgcggtccca agggcacttc tcccggagct
     1021 catccataat ggtcaaaagc ctgtcgaatg cctctcttgc cctctcgttt cttgtgatgt
     1081 tatctgtttt cttatccata ttgctaaatt tattgaactt gctagcggtt agtgaactta
     1141 accataatgc tcgaacctac agaaagagcg agaagctcgg tcagaaatcc gttgcgaatg
     1201 gcaatttcca agaggttaag cgagttgaag agagccaaca tgtcgccatc ctctacatcg
     1261 gcgtagtttt caacaatttc ctcaattttg tagctgaagc tttgcacgta gatggaaaat
     1321 ttccggccct ttcgcacctg ttcaaactgc tccctggtga tgttggttat tgcattttgg
     1381 taggaatcaa catagataac gcttcccgtt attgacgatt cgtcggatgc tgccctgatg
     1441 ttaatgttct gcttgtaggt gccggctatc agcgcaatat cggccaagtt ggagttaaaa
     1501 agtagcatgt tcatggcgct ggcaacagcg ctaagcgacc caaagctgct atgttcttca
     1561 aagggaatgg agtaaactct gtccggagca acttcggtaa tcaggccaac tatgccgttg
     1621 tcgggcacaa tcatgaaatg accctttagc tcgaacagca gaagctgctt gctggttgtg
     1681 ctatcgctgt tgaccatgca aatatgtatg gttcccggcg gaaagttctc aaagcaaccc
     1741 cgcatgataa atgctgcctg gatgtaatcg aatggcggaa ccctgtggct tagctccaca
     1801 atgcggaggg taggctcaat ggagagcaac ctgccgcata gaatgccgac atagtagtcg
     1861 ttttggttcc agtctgtggt taaggttact atccgcaaat tatccatggg atttgctaaa
     1921 agcattacaa aagtaatgga gtaatcggga atattgaaaa ttagcatcct atttcaatag
     1981 catcaccacg caaatagcaa gtatccaacc acataactat atggactgat tttaaaaaag
     2041 accagggaat cattatgaat aacgtaggta aatcaagaag tgcgttggaa tgcctaaaac
     2101 cgaacctaga cgccaaacgg taagaaaaaa aatcaattat actaaaaacc tacactcttg
     2161 cggaagttac attagctaaa aaattgattg ctgaaacttt aaaaatggct g
//
LOCUS       DAOOQX010000333         2202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_61228_length_2202_cov_3.524453, whole genome shotgun sequence.
ACCESSION   DAOOQX010000333 DAOOQX010000000
VERSION     DAOOQX010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61228_length_2202_cov_3.524453"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PLX53_06015"
     CDS             <1..721
                     /locus_tag="PLX53_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOY72675.1"
                     /translation="PRDITARAIDSEMKQRGDDFVYLDVTFKDPDDTINHFPNIYQKC
                     LSVGIDVTKDMIPVVPAAHYLCGGIKVDMNGQTAIKRLYAIGECSSTGLHGANRLASN
                     SLMEAIVYAERAARHSLATFKSINNSTRIPDWDYEGTTHNEEMVLITQSYKEMQQIMT
                     NYVGIVRSNLRLSRALNRLDIIFEETEELYKKSILTRELCELRNLIQIGYLIIKDAQN
                     MHESRGLHYSIDYPRQMGTEF"
     gene            823..1935
                     /locus_tag="PLX53_06020"
     CDS             823..1935
                     /locus_tag="PLX53_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOY72676.1"
                     /translation="MPKRKRHYSENPARRIKVGTAIIPVIIGLGVVFLMLFREFNPQA
                     FLQISFTWRSFFWILVAALLMAGRDMGYTFRLMTLSEGKFKFWKAFRVIMLWEFTSAI
                     TPSAVGGTSVAVVYVNKEGLTVGRSSAVVMITSFLDELYFVVMFPILVLLTGPHELFT
                     LASSPAGGMAKDLIIFSIVGYSLKLVWVLGLSYGLFINPRGLKWLIMMIFKLPLLRRW
                     KQGANKAGSDIITSSEEFKKKPFSFWAKAVASSFLSWSSRFLVINALLLAFFDVNHHL
                     LIFTRQLVMWIIMLVSPTPGGSGFAEFVFTRYLSDFIPVGAESLTSIAVGMAMLWRLI
                     SYYPYLFIGAIVFPRWVRKKFGREKGHRSREIATTK"
BASE COUNT          571 a          484 c          502 g          645 t
ORIGIN      
        1 tccccgcgat attactgccc gggccataga ttcagaaatg aagcagcgtg gcgatgactt
       61 cgtttacctc gacgttacct ttaaggatcc agatgacacc atcaaccact tccccaacat
      121 ttaccagaaa tgcctatcgg ttggtattga tgttaccaag gatatgatac ctgttgtgcc
      181 tgcagcccac tacctttgcg gtggaattaa ggttgacatg aatggtcaaa cagccattaa
      241 aaggctttat gccattggcg aatgctcgtc aaccgggctg catggggcta accgacttgc
      301 ttccaactcg ctgatggaag ctattgttta tgccgaaagg gctgcccggc attcactggc
      361 aacttttaag agtattaaca acagcaccag aatccccgac tgggattacg agggaaccac
      421 ccacaacgag gaaatggtgc ttatcactca aagttacaag gaaatgcagc aaattatgac
      481 caactatgtg ggtattgttc gttctaacct tcggctttcg agagctctta accgcttaga
      541 catcatattt gaagaaaccg aggaacttta caaaaaatcc atactcacca gggagttatg
      601 cgagctgcgc aacctcattc aaattggcta cctaattatt aaggatgccc aaaatatgca
      661 cgaaagcagg gggcttcact actcaattga ctacccacgc cagatgggta cagagtttta
      721 gcaggaagca acaacacctt tattgaaatg gagtggtagt taaaagtttg ttatgattgt
      781 gaatagcgtt agcaccaagt agcctttacc tccagactgc tcatgccgaa aagaaaacgc
      841 cactattctg aaaatccagc ccgacgcata aaggttggaa ctgctatcat ccccgtaata
      901 attggtctgg gggtggtttt tttaatgctt ttcagggagt ttaaccctca agcctttttg
      961 cagataagct ttacctggcg ttcattcttt tggatacttg tagccgcact tttgatggca
     1021 ggaagggata tgggctatac cttcaggctt atgacccttt ccgaagggaa gttcaagttc
     1081 tggaaagcct tccgggtaat aatgctatgg gagtttacct cggccattac gccttctgct
     1141 gttggcggaa ccagcgtagc tgttgtctac gtcaataagg aaggtcttac ggtgggtcgc
     1201 agctcggcag ttgttatgat aacctccttt ctggacgagc tatattttgt agtaatgttc
     1261 ccgattcttg ttcttttaac cggccctcac gaacttttta cccttgcttc ttctcccgct
     1321 ggtggtatgg cgaaagacct tataattttc agcatagttg gctattcgtt aaagttggtt
     1381 tgggttttag gcttatcgta tggccttttt ataaacccac gggggctgaa atggcttatc
     1441 atgatgattt ttaagctgcc gttgctgcgc aggtggaaac agggtgcaaa caaggccgga
     1501 agcgatataa taacgagctc ggaggagttc aagaaaaagc cattctcttt ttgggctaaa
     1561 gccgttgctt cttcattcct ttcatggtcg tcgaggtttt tggtgataaa cgcacttcta
     1621 cttgcatttt ttgatgtaaa ccaccacttg ctaattttta cccgacagct ggttatgtgg
     1681 attatcatgc tggtaagccc tacgcctggg ggaagcggtt ttgccgaatt tgtattcacc
     1741 cgctacctgt cagattttat tcccgtagga gcagaatcgc ttacttccat tgctgttgga
     1801 atggccatgc tctggcggtt aataagctac tacccctacc ttttcattgg tgcaatagtt
     1861 tttccgcgct gggtaagaaa aaagtttggg agggaaaaag gccacagaag ccgagaaatt
     1921 gcaaccacaa aataattagg gttgattttt gtggaattca taccctaggt tcgtcttgtt
     1981 aagtcagcat tctggttagg cagcacaaca taacgagtca tacccatttc acgttgaatc
     2041 atacctacgc tgtgctgtaa taaaaaagac ctgctgttag tttagcaggt ccgttcttat
     2101 tttgattggc attgtgaaca gggctatata acaccgagtt tttttccaat tttagtaaac
     2161 ttatcaatgg cgaataggac attttcccta ctgtgggcag ca
//
LOCUS       DAOOQX010000334         2198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_61396_length_2198_cov_3.795147, whole genome shotgun sequence.
ACCESSION   DAOOQX010000334 DAOOQX010000000
VERSION     DAOOQX010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61396_length_2198_cov_3.795147"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLX53_06025"
     CDS             <1..186
                     /locus_tag="PLX53_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOY72677.1"
                     /translation="GYSGWGKDQLKREIDEDSGVVANFDSQLMAKGAYDSWYFAVQQL
                     GSRFKAWTLYPENPALN"
     gene            complement(159..1040)
                     /locus_tag="PLX53_06030"
     CDS             complement(159..1040)
                     /locus_tag="PLX53_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOY72678.1"
                     /translation="MASRNYIWLNGQFFDGGNPVINFENRAFRYGDGVFETIHAFGTQ
                     PRHLPLHFRRLLQGMKILELVAPTWLTEQNLELAITKLLNKNRLFDSARVRVTVYRDD
                     GGFYIPSTSNASVLIDTQPLAAKMYTLNQQGCIVGIYDEIKKPLNTLSGVKSCNALLF
                     VKAGLYCMANNLDDCIILNEQGRVAEAISSNVFVVRGGKLYTPSLAEGCIPGIMRELV
                     CTVAPTVGIEVNNQVAINPSAFDSCDEVFLTNAISGIKWVVGIGTRRYFNRVSRTLSE
                     ALNRFTFPDQFRAGFSG"
     gene            complement(1047..1460)
                     /locus_tag="PLX53_06035"
     CDS             complement(1047..1460)
                     /locus_tag="PLX53_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOY72679.1"
                     /translation="MKICEGWLQSGEHRLVAIMARYRGGWIWVMNRNRSTWEQPGGHI
                     ETGEEPDETASRELFEETGALDFSMVPLFDYTIQQGSARSDCRMFYAAVTKLGALPQS
                     EIQKIGMFDSPPDELTYEGVQLQLFEAALGYVSES"
     gene            complement(1490..2107)
                     /locus_tag="PLX53_06040"
     CDS             complement(1490..2107)
                     /locus_tag="PLX53_06040"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOY72680.1"
                     /translation="MTYAIIIITSLVSAVALRNGELMQRLLLSPYLVFRNRQWYRLIT
                     HALVHANYLHLIVNMIVLLSFGKAVESILRQLNMEGVIGHPALHYLMLYLGGAVVATL
                     TTLKKFRNDYSYQSVGASGAVSGMIFFCIFFYPLNKLYLMGILPIPGILFAVAYLIYS
                     NYMSRKGGDNINHDAHFVGAVFGFIYPLLISPKLITVFLKQLGLM"
BASE COUNT          553 a          570 c          544 g          531 t
ORIGIN      
        1 ggctactccg gatggggcaa ggatcagcta aaaagggaga tcgacgagga ttcaggggtt
       61 gttgccaact tcgactctca gcttatggcg aaaggagctt atgatagctg gtactttgcc
      121 gttcagcagc tggggtcaag gtttaaggcg tggaccctct accccgaaaa tccagcccta
      181 aactgatcgg gaaaggtgaa gcggtttagt gcctcgctta gcgttctact cacccggttg
      241 aagtaccttc gggttccgat gcctaccacc catttaatcc ccgatatggc attggtgagg
      301 aaaacctcgt cgcagctgtc gaatgccgac gggttgatgg caacctggtt gttaacctct
      361 attccaacag ttggggcaac agtgcacacc agctcccgca tgatacccgg tatgcacccc
      421 tcagccagcg agggggtgta gagttttccg cctcttacca cgaacacgtt ggacgaaata
      481 gcctcggcaa cccttccctg ctcattcagg atgatgcaat cgtccaggtt gttggccatg
      541 cagtatagcc cggccttaac aaaaagtagc gcattgcagc tcttaacgcc cgaaagcgta
      601 ttcagcggtt ttttgatctc atcgtaaatt ccaactatgc agccctgctg gtttagggtg
      661 tacattttgg ctgccaacgg ctgtgtgtca ataagaacgc ttgcgttgga ggtagaaggg
      721 atatagaatc ccccatcgtc gcggtaaacc gtaaccctca cccgtgcact gtcaaaaagt
      781 cggtttttgt taagcagctt cgtaatggct agttcaaggt tttgctcggt aagccaagtt
      841 ggtgcaacga gctccaggat tttcattccc tgcagcaggc gcctgaagtg caggggaagg
      901 tgacgtggtt gcgttccaaa ggcgtgaatg gtttcaaaaa ccccatcacc atagcgaaac
      961 gccctgttct caaaattaat gacagggtta ccgccgtcga aaaactggcc attaagccaa
     1021 atgtagttcc tgcttgccat tcggcattaa ctttcagata catacccaag cgcagcctcg
     1081 aaaagctgta gctgaacccc ctcgtatgtc aactcgtctg gagggctatc aaacattcct
     1141 attttctgaa tctcactctg gggaagagcg ccaagcttgg ttaccgcggc gtaaaacatc
     1201 cggcaatcac ttctggcgct tccctgctgg atggtgtagt cgaaaagcgg aaccatgctg
     1261 aaatccaagg ctccagtctc ctcaaacagc tccctgctgg cggtttcatc tggttcttct
     1321 ccggtttcaa tatgtcctcc tggctgctcc caggttgagc ggttacggtt catcacccaa
     1381 atccaccctc cacggtatct ggccatgatg gcaacaagcc ggtgttcccc actctgtagc
     1441 catccttcac atattttcac aaccaagggt tccatatacc ggcatcaagc tacattagtc
     1501 caagctgctt taaaaaaacg gtaatcagtt tagggctgat gagcagcgga tagataaagc
     1561 caaatacagc acctacgaag tgagcatcgt ggttgatgtt atcgccaccc tttcggctca
     1621 tgtagttgga gtaaatcagg taggcaacgg caaacagaat accaggaatt ggcagaatgc
     1681 ccatgaggta gagcttgtta agcgggtaga aaaaaatgca gaagaatatc attcccgaca
     1741 cagcccccga agcgccaacg ctctggtagg agtaatcgtt tctgaacttt ttgagggttg
     1801 taagggtggc aacaactgca ccacccaggt agagcattag gtagtgcagg gcaggatgac
     1861 cgataacccc ctccatgttc agctgcctta ggattgactc caccgccttc ccaaacgaca
     1921 gcagcacaat catgttgaca atcaggtgca ggtagtttgc gtgaactaaa gcgtgggtaa
     1981 tcaaccggta ccactgcctg ttccggaaca ccaggtaggg actcaggagc aatcgctgca
     2041 tcaactcccc gtttcggagc gcaacagccg ataccagcga ggtgatgatg ataatggcgt
     2101 aggtcatgct agtgaattat cttaaaaatt aactccttca gcaactcacc atggtcggtt
     2161 gatccattgt tcattccttt tgaacgcatg tcgtagtc
//
LOCUS       DAOOQX010000335         2195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_61530_length_2195_cov_3.137850, whole genome shotgun sequence.
ACCESSION   DAOOQX010000335 DAOOQX010000000
VERSION     DAOOQX010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61530_length_2195_cov_3.137850"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLX53_06045"
     CDS             <1..247
                     /locus_tag="PLX53_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72681.1"
                     /translation="DINILRENRFDYGLMGGVGLSHDFQWGTIQAEARFGYGLGDLYD
                     YSYSGMPSESRAIIQNFSLSYLYRFGQHNTQQEVKQP"
     gene            501..1166
                     /locus_tag="PLX53_06050"
     CDS             501..1166
                     /locus_tag="PLX53_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72682.1"
                     /translation="MKAWKEGDYRLQQVISNTRLAPGAFLLKFRKQFDFSAGQIVALG
                     VSTDIEPRFYSIASSEEDELISVLYTEKPDGKLTPMLSMLNPGNPILVSKPFGAFTSF
                     KSEPVFVAAGTGVAPFISALLSNKVRGATLIHGAPYPEYFYFSDYLSEKLGRNYIQCC
                     SRCMPGSHYVGRVTSYLLQWDELDPNRNYYLCGSTEMVVETRSLLISRGVPPDRINAE
                     IYF"
     gene            1255..>2195
                     /gene="rlmN"
                     /locus_tag="PLX53_06055"
     CDS             1255..>2195
                     /gene="rlmN"
                     /locus_tag="PLX53_06055"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOY72683.1"
                     /translation="MPELLPLYGKTLAELQDIVSQEGLPPYAAMQLADWLYKKNVESI
                     EAMTNISKSAREQLVGRYYVGLSKPEGVSASADGTKKYLFRSGNGKFVEAAYIPETGR
                     ATLCVSSQAGCKMGCLFCMTGRQGFQGNLSSGEIINQLRSIPEFKNVTNIVYMGMGEP
                     FDNLENVLRSTEVLTSEWGMGWSPKRITVSSIGIIPAMNTFLSRSSCHLAISLHSPFD
                     EERRMLMPIQNVYPIADVVEEIRKANIAGQRRVSFEYIMFDGLNDSPRHVREISRLLH
                     NLRCRVNLIRFHPIPESALRSASDETIIKFRDALTSKG"
BASE COUNT          580 a          494 c          513 g          608 t
ORIGIN      
        1 ggacatcaac attcttcgcg aaaaccgttt cgactacggg ttgatgggtg gtgtaggcct
       61 aagccatgac ttccaatggg gaaccattca ggctgaagcc aggtttggtt acgggttggg
      121 tgatctttac gattactcgt attccggcat gccaagcgag tcaagggcta tcatacaaaa
      181 ttttagcctg agctaccttt accgatttgg ccaacacaat acacagcaag aagtcaaaca
      241 gccttaagtt ataattttct cacctgaacg caggagtgtg tgttttcaaa aatttactgt
      301 aaactatttg tagttttgaa tgttactgct ttcgttgtgg taaacctatg ttttagtcta
      361 acgtgcttgc ttgccagggt taagcaaccc gtgcttcagg aataattgct gcacattgtt
      421 aaggcattaa ggtaatttac cccctactag gcttatgtta gctgataaat cgtagtacta
      481 aaattatttc cgattacttc atgaaagcat ggaaggaagg cgattataga ttacagcagg
      541 tgatttcgaa tacccgacta gccccgggag cgtttcttct gaaattcaga aaacagttcg
      601 attttagtgc agggcagatt gttgcccttg gagtttctac cgatattgaa ccaaggttct
      661 acagcattgc ttcttccgag gaagacgagt tgatatcggt tctctatacg gaaaaacccg
      721 atgggaagct aactcctatg ctgtcaatgc tcaatccagg taacccgatt ctggtgtcaa
      781 agccatttgg agcatttacc agctttaaat ccgaaccggt gtttgttgcc gccggtactg
      841 gggttgctcc tttcatttct gcactcctat ccaataaggt tagaggggca actttaatac
      901 atggtgctcc atatcccgag tacttttact tttccgatta tctttccgag aagctaggaa
      961 gaaactatat ccagtgctgc tcgaggtgta tgcccggaag ccattatgtt ggtcgtgtta
     1021 ccagctacct gctgcaatgg gatgagctag atccaaaccg aaattactac ctttgtggca
     1081 gcaccgaaat ggttgttgaa acaaggagct tactaatttc aaggggtgta ccacccgata
     1141 gaataaacgc tgaaatttac ttttaaacct attggtagct tttcacaatt cattaattta
     1201 caatttacca gaaaatcata ctacctcgat tgtttccatt aacttcttaa tcttatgccc
     1261 gaactactcc cgctatatgg aaagacgctt gccgagctac aggatattgt tagccaggag
     1321 gggttgcctc cgtacgccgc tatgcagctt gccgactggc tttacaaaaa gaatgtggag
     1381 agcatcgagg cgatgaccaa catttccaag agcgccagag aacagcttgt cggtaggtat
     1441 tacgtcggcc ttagcaagcc cgaaggcgtt tcagcttcgg ccgatggaac caaaaaatac
     1501 ctttttcgga gcggaaatgg aaaatttgtc gaggcagcct atatacctga aaccggaagg
     1561 gcaacacttt gcgtatcgtc acaggccggg tgcaaaatgg gttgcctgtt ctgcatgact
     1621 ggcaggcagg gttttcaggg aaatctttcc tcaggtgaga ttatcaacca gctccggagc
     1681 atacccgagt ttaaaaatgt tacgaatatt gtttacatgg gaatgggcga accattcgac
     1741 aacctggaga atgttcttcg cagcaccgaa gtgctgacct ccgaatgggg tatgggctgg
     1801 agcccgaaac gcattaccgt ttcatccatt ggaattatcc ctgcaatgaa caccttccta
     1861 tcccgaagta gctgccattt ggcaattagc ctgcattctc catttgacga ggagcgtagg
     1921 atgctgatgc ctattcagaa tgtttacccg attgccgacg ttgtggagga gatccgtaag
     1981 gccaacattg cggggcaacg aagggtatcg ttcgagtaca tcatgtttga tgggcttaac
     2041 gacagtccac ggcatgttcg ggaaatatcc cgactgttgc acaacttacg atgtagggtt
     2101 aacctgatcc ggtttcaccc aattcctgaa tcggcccttc gcagcgcatc cgatgaaacg
     2161 atcataaagt ttcgcgatgc ccttacctct aaagg
//
LOCUS       DAOOQX010000336         2190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_61730_length_2190_cov_3.438407, whole genome shotgun sequence.
ACCESSION   DAOOQX010000336 DAOOQX010000000
VERSION     DAOOQX010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61730_length_2190_cov_3.438407"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..244
                     /locus_tag="PLX53_06060"
     tRNA            173..244
                     /locus_tag="PLX53_06060"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Glu,seq:ttc)
     gene            273..527
                     /gene="rpsT"
                     /locus_tag="PLX53_06065"
     CDS             273..527
                     /gene="rpsT"
                     /locus_tag="PLX53_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOY72684.1"
                     /translation="MANHKSAEKRIRETETRRLHNRYYAKTTRNALKALRNTTNKAEA
                     IEMLPKVSSMLDKLAKKHIIHRNKAANLKSSIQKHVNALQ"
     gene            complement(584..1390)
                     /locus_tag="PLX53_06070"
     CDS             complement(584..1390)
                     /locus_tag="PLX53_06070"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4163 domain-containing protein"
                     /protein_id="HOY72685.1"
                     /translation="MLKSRIIRLLSLAVLMGLVYFLFNTVRATYTPNLSVAPDSLVFT
                     RGCVEPDSSCFRILLKFPVVQGKRAARIQKMIAYDYLKLLSDSTKVKGVNQFKEVLTR
                     LTESYDSAYVDYLSLFPGSNGISWYLNADYDVLLNDGKVLTIQYRYEDYLGGAHGMYG
                     YHYQNIDLRKSRPLSLNDIVSNPKQFSELAEKLFKGRVGVDHLNGYWFEGGKFSLPRE
                     FGLSENGIVLHYNVYEIASFADGDLVFEIPYSSMGGMLSKRFRYLAEEGK"
     gene            1765..>2190
                     /locus_tag="PLX53_06075"
     CDS             1765..>2190
                     /locus_tag="PLX53_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72686.1"
                     /translation="MNSKSIKWLLTVGVIAIIILQVGCKGKQDVEISTDPLFGEYISA
                     YTSGMISAHSAITIKLTKPSSKFTFNGDEVHEGYFSFSPSVKGKAYWVDDQTLEYRPT
                     EVMRSGKVYSSSLALSKLFKGVEPKLKTFRFSFRVIPQSV"
BASE COUNT          655 a          493 c          418 g          624 t
ORIGIN      
        1 tctgcctatc ctgaacctca tagaatcgcc cggtatttgg tttaatgcag gcctttcggt
       61 tcgctatgct tttaagtaaa aacagcataa attttttccc atttatttgt agaaaataaa
      121 aattcactta cctttgcacc cgccttggta ggcaatatgc tacttagagt aaggcccgtt
      181 cgtctagggg ttaggacgtc aggttttcat cctggtaaca gcggttcgat tccgctacgg
      241 gctacgaata acgaacctat ttataaacgg atatggcaaa tcacaaatca gcagaaaaac
      301 gaatcaggga aaccgaaacc cgaaggcttc acaaccgcta ctatgccaaa actacacgta
      361 acgccctgaa agctttacgt aacaccacta acaaggcaga agcaatagag atgcttccca
      421 aggtttcatc aatgctcgac aaacttgcca aaaagcatat tattcatagg aataaagctg
      481 caaatcttaa gagttccata caaaagcatg taaatgccct gcagtaagaa aggcttaatt
      541 ttccatgaac tgcactttgc gcctcccaca atcgaggcgt tttttattta ccttcttcgg
      601 ctaggtacct gaatcgtttt gaaagcatcc cccccatgct ggagtatgga atttcaaaga
      661 ccaaatcccc atcggcaaag ctggctattt catacacgtt gtagtgaagc actattccat
      721 tttcgctaag tccaaactcc ctgggtaggc tgaattttcc tccttcaaac cagtagccat
      781 tcaaatgatc aacccctacc ctacccttaa agagtttttc ggccaattcg gaaaattgct
      841 tggggttgct tacaatatca ttaaggctga gcggtctgga cttacgcagg tctatgtttt
      901 ggtaatgata gccatacatt ccatgtgctc ccccaaggta atcttcgtag cggtactgga
      961 tggtcaacac ttttccatca ttcaacagga cgtcgtaatc ggcatttaaa taccacgaaa
     1021 tgccattgct gccaggaaat aacgataggt aatcaacata agcactatca tagctttcgg
     1081 ttaaccgggt aagaacttcc ttaaactggt taactccctt tacctttgtg ctgtccgata
     1141 aaagtttcag gtagtcgtag gctatcatct tttgaattcg ggcagccctt ttcccctgaa
     1201 caaccggaaa tttaaggagt attctaaaac aggaagaatc gggttctacg catccacgtg
     1261 taaacacaag agaatctgga gccaccgata agttgggtgt ataggttgcc cttacggtgt
     1321 taaatagaaa ataaaccaac cccataagaa ctgctaacga tagaagacgt attattctgg
     1381 acttcagcat attactattt tccctttaaa tattgaacca gctccaggcg agctattaaa
     1441 cctatttctt tactgctcaa atatagctcg ataatttcga aaaatcttat gcttggtctg
     1501 ccccattcat cacccgtcgt aaaccttcaa atgccgctcg cctcccaacc gattgaacta
     1561 catataaaat gcttaaatac agatgtacta cccataacct tgcggtattc gggttctaaa
     1621 agcgcattgt ttttaaagtt cctttacctt aagaaagtag atggatgttt gtgtgttacc
     1681 aatatttgtg ggcatttcaa tattaatgcg gtaaactcaa aatcttgagt aattttgggt
     1741 agtagtataa atcatccttc aagcatgaac agtaaatcaa tcaaatggct tttaactgtt
     1801 ggggttatag ccataatcat ccttcaggtt ggatgtaagg gcaaacagga tgttgaaatt
     1861 tcgactgatc cgctatttgg agagtacatt tcagcatata cctcgggaat gatttcggca
     1921 cactccgcca tcacaattaa gcttacaaag ccaagcagca agtttacttt caatggcgac
     1981 gaggtgcacg aaggctattt cagcttttca ccatcagtaa agggtaaagc ctactgggtt
     2041 gatgaccaaa ctctagagta tagaccgaca gaggtaatgc gttcgggcaa agtttactcc
     2101 tcttccctgg cattaagcaa gctttttaaa ggtgtagaac caaagctaaa aacgtttcgc
     2161 tttagcttca gggtaattcc acaatctgtt
//
LOCUS       DAOOQX010000337         2189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_61784_length_2189_cov_2.496720, whole genome shotgun sequence.
ACCESSION   DAOOQX010000337 DAOOQX010000000
VERSION     DAOOQX010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61784_length_2189_cov_2.496720"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1335)
                     /locus_tag="PLX53_06080"
     CDS             complement(115..1335)
                     /locus_tag="PLX53_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOY72687.1"
                     /translation="MEFEQRTLHNGIQIVHKRVSSPVAHCCILINAGTRDELDNEHGI
                     AHFIEHVLFKGTKKRRAFHIMSRLEDVGGELNAYTTKEETVIHASFLKQDFNRAMELI
                     TDIAFHATFPEKEIAKEKEVVIDEINSYKDSPYELIFDDFEELIYPNHPFGRNILGTK
                     NHVSRFNRKGIAAFISRCYNTDQMVFCSVGDIPFTRVSRLADKYLNGISANKRSKPRL
                     ELGNYRPQDKIIKKSTFQTHCIIGTTAYDLQNPKRIAMHLLNNILGGPGMNSRLNLAL
                     REKHGFAYNVESSYTPYTDTGVFTIYFGTDKANVEKSHHEILKELARIREQRMGVMQL
                     FKAKRQLIGQTAIAADSNENLMLSAAKSFMVYQKPEDLTEVFNRIENISSSDILEVAN
                     EILSENRLSTLIYR"
     gene            1607..1679
                     /locus_tag="PLX53_06085"
     tRNA            1607..1679
                     /locus_tag="PLX53_06085"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1640..1642,aa:Phe,seq:gaa)
     gene            complement(1922..>2189)
                     /locus_tag="PLX53_06090"
     CDS             complement(1922..>2189)
                     /locus_tag="PLX53_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72688.1"
                     /translation="PTTGLHFEDVRILLDVLNKLVDRGNTVIVIEHNMDVVKVADYII
                     DLGPEGGAEGGKVVCTGTPEELIKSACGYTATYLKRHLNREFNT"
BASE COUNT          541 a          469 c          513 g          666 t
ORIGIN      
        1 gagggttaca ggtggaaagg taggtgaacc gggtcagctc catcagggta tccgccattg
       61 gcgaggtatg gcagctcagg tattcctcca tgtcgttatc aaccttagcc atatctaccg
      121 gtatattaac gttgaaagcc tgttttctga aagaatttcg ttggcaacct cgagtatgtc
      181 ggacgatgaa atgttttcta tgcggttgaa aacttctgtc aggtcttcgg gtttttggta
      241 aaccataaaa ctttttgcag ccgaaagcat gaggttttcg ttgctatcgg cagcaatggc
      301 ggtttgccca ataagctgtc tttttgcctt aaaaagctgc atcaccccca ttcgttgctc
      361 cctaattctg gcaagctcct taagaatttc atggtgcgat ttttcgacat tggccttatc
      421 ggtgccaaag taaatggtga acactccggt gtcggtgtat ggtgtgtatg acgattccac
      481 gttgtaggca aaaccatgct tctcgcgtag tgccaggttg agccttgagt tcatgccagg
      541 ccctccaagg atgttattga gcaggtgcat ggcaatgcgc tttgggtttt gcaagtcgta
      601 ggcagttgta ccaataatgc aatgggtctg aaatgttgat tttttaatga ttttatcttg
      661 gggtctatag ttgccgagct ctaaacgggg tttactcctt ttgttagcag aaatgccgtt
      721 taggtactta tctgccaacc tgctcacacg tgtaaaggga atatcgccta ccgaacagaa
      781 taccatttgg tcggtgttgt aacagcggct tatgaacgct gcaataccct tgcggttaaa
      841 cctgcttaca tggtttttgg tgcccagaat attcctacca aaggggtggt tagggtagat
      901 aagctcctca aagtcatcaa atattagctc gtaggggcta tccttatacg agtttatctc
      961 atctattacc acctcctttt ccttggctat ctccttttcg ggaaaggtgg catgaaaagc
     1021 gatgtcggta atgagctcca tggccctgtt gaagtcctgc tttagaaagc tggcgtgaat
     1081 aaccgtttcc tccttggtgg tgtaagcgtt aagttcacca ccaacgtcct caaggcggct
     1141 catgatgtga aaagcccttc tttttttggt tcccttaaac agaacgtgct caataaaatg
     1201 ggcaataccg tgctcgttgt ccagctcatc gcgagttcct gcgtttatca gaatgcagca
     1261 gtgggcaaca ggtgatgaaa cccgtttgtg gacaatttga ataccgttat gaagggttct
     1321 ctgctcaaat tccattatac cccggtaaat ttgaatgcaa acctactata aataggcaac
     1381 ttgtcaaagt aaaagagctg ccgctaaaat gtggcatacg aaaggtatcc gttacccaag
     1441 cccgtaatgt aacggctttg gttactgcca tcaaggttat atccttagca ttcagctaca
     1501 tgttgggttg aattgcatgg aagcacctat taccggtagt aaaattatgg aaccaatgtt
     1561 tgcagataca aaaatatcct atatatttgc accgcaattg aacgttggtg ccatagctca
     1621 gttggtagag caaaggactg aaaatccttg tgtccgtggt tcgattccac gtggcaccac
     1681 gtaaaacctc cggatatccg gaggtttttt gatttaaagg attttacaaa ctcttgttta
     1741 ccctgttttt ttacactgga agttgccggg gctaagccag tgttgttcat ttatggatta
     1801 tacatttaca ggttttcgct ttaatctgtt atggataagt ataatttgtt gcctggaaaa
     1861 tgaaaatgca ggattggttt aaaagggttt ttatactcct aaggcatagg tagaatctta
     1921 tttaagtgtt aaattccctg ttgagatgcc ttttcaggta ggtggcggtg tagccgcatg
     1981 ccgacttaat aagctcctcg ggggtacctg tgcaaaccac ctttccacct tctgctcctc
     2041 cctccgggcc aaggtctata atgtagtcag ctacctttac cacatccatg ttgtgctcga
     2101 tgacaattac agtgtttccc ctgtcaacca gcttgttcag cacgtcgagt aggatacgca
     2161 catcctcgaa gtgaaggccg gtagtgggc
//
LOCUS       DAOOQX010000338         2184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_61984_length_2184_cov_2.720526, whole genome shotgun sequence.
ACCESSION   DAOOQX010000338 DAOOQX010000000
VERSION     DAOOQX010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61984_length_2184_cov_2.720526"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1406)
                     /locus_tag="PLX53_06095"
     CDS             complement(12..1406)
                     /locus_tag="PLX53_06095"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY72689.1"
                     /translation="MRAELLKKRALTRMVRLASNLSDEKLIRMVELSQRHLMRGVTDE
                     RVRFTAEKIKKLFEEKHPATELAKSTFRRVSKESRKRIVENFFINAGVTGRQRQEELK
                     RELGFGMPWFFVISPTAKCNLKCKGCYAGEYTKSEDLSFELVDRIFTEAKELGMYFIT
                     ISGGEPYMWPPLLDIFRKHSDMFFQTYTNGTLLTKDMAQKLAKLGNVAPAISVEGFEK
                     ETDARRGNGVFQRVMDAMDNLREAGVVFGYSVTPTRLNSELLVSDEFVDLMIQKGCVF
                     GWYFQYVPIGRKPDVSLMATPEQRNYIRQRTKEIRNTKPIFIGDFWNDGPIVGGCLAG
                     ARHRGYFHINCKGDVEPCVFLQFSVDNIKDKKLLDVIQSPFFRAIQDAQPYCENKNLL
                     TPCALIDNPQVLRDVVKQHNARPSYNSSYEVISNPDVCNFLDAYSRDYKKITDPIWEN
                     EVSLKYQSWKERFS"
     gene            1834..>2184
                     /locus_tag="PLX53_06100"
     CDS             1834..>2184
                     /locus_tag="PLX53_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HOY72690.1"
                     /translation="MGSDYRILPHVNSPNDLKGMKPEQLVELSEELRRFIVEVVSTNP
                     GHLGASLGVVELTVALHHVFNTPDDKLIWDVGHQAYAHKILTGRRDVFNTNRRYKGIS
                     GFPKRSESPYDAFGV"
BASE COUNT          593 a          543 c          459 g          589 t
ORIGIN      
        1 caaaatttct gctagctgaa gcgttccttc caggattggt acttcaagct aacctcgttt
       61 tcccaaattg gatcggtaat tttcttgtaa tcccttgaat aggcatctaa aaaattacaa
      121 acatccggat tagaaatcac ctcgtagctg ctgttgtagg aaggcctggc gttatgctgc
      181 ttaacaacat cccttaaaac ctggggatta tcaattagag cgcagggggt gagaaggttc
      241 ttattttcac aatagggttg ggcatcctga attgccctga aaaatgggga ctgtataacg
      301 tcgagcagct ttttatcctt aatgttgtcc accgaaaact gcaggaaaac gcagggctcc
      361 acgtcgccct tgcagttgat gtggaagtat cccctgtgcc tggcaccggc aaggcatccg
      421 cccacaattg gaccatcgtt ccagaagtcg ccgataaaaa ttggctttgt attccttatc
      481 tccttggtgc gctgcctaat atagtttctt tgctcgggcg ttgccatgag cgaaacatcc
      541 ggtttacggc caattggcac gtactggaag taccagccaa acacacaccc cttttgaatc
      601 atcaagtcaa caaactcgtc ggaaactagc aattcggagt tgagccgtgt tggtgtaaca
      661 gagtatccga ataccacacc ggcctcacgc aggttgtcca tggcatccat caccctttga
      721 aaaacaccgt tgcccctacg ggcatccgtc tccttctcaa acccctccac cgaaattgcc
      781 ggtgccacgt tgcccagctt cgccaacttt tgggccatat ccttagttaa cagcgtgccg
      841 ttggtgtagg tttggaaaaa catatcgcta tgcttcctga aaatatcgag cagaggaggc
      901 cacatgtaag gttccccacc tgatatggtg ataaagtaca tccccagctc tttggcctcg
      961 gtaaaaatgc ggtcaaccag ctcgaatgaa aggtcctcgg acttagtgta ctccccggca
     1021 tagcacccct tgcacttgag gttgcacttt gccgttgggc taataacaaa aaaccacggc
     1081 atgccaaatc ccagctcacg tttcagctcc tcctgccttt gccttccggt aacccccgca
     1141 ttgataaaga aattttcaac tattcgcttt cggctctcct tcgatactct tctgaatgtg
     1201 cttttggcca gctcggttgc gggatgtttc tcctcaaaga gcttcttaat cttttctgcc
     1261 gtaaacctca ccctttcgtc ggtaacccct ctcatgaggt gccgctgcga aagctccacc
     1321 atcctaatca gcttttcgtc cgatagattt gaagccagcc taaccatccg ggttaatgct
     1381 ctcttcttta aaagttctgc tctcatggct taaaaaatta atgaacaaat tcgtatacga
     1441 aaaacttatt ttaaaaacct gaataggtta ttgaaaattc aagccatccc tttattctat
     1501 gagccttgaa cgtgttcaaa aggtagtgaa taaaaccata ggcatccagc cttttaacat
     1561 aattttagat tttttaatac tgatgagagg gcaacccccc atgtaacata ttctgtctgt
     1621 aattaaagtg ttataggaca acccattagt ataaatcaaa cataaaaagc agaagcttac
     1681 ataagcctaa atgatgaacg catcaaaatt atgatggtac ttaagctaac cctttcactc
     1741 gtcaggtagc aactccgtgt gccctataaa tattttcaga aaagagcaac attttcttaa
     1801 ctttgcggtt aaaggaatat aagtggagca gcaatgggca gcgactatag aatattaccc
     1861 catgttaact cgcccaacga tctgaagggg atgaaaccgg agcagctcgt tgagcttagc
     1921 gaggagttgc gcagatttat cgttgaggtg gtttcaacta atcccggcca cctaggagcc
     1981 agcctggggg tagttgagct gacagttgcc cttcatcatg tattcaatac acccgacgac
     2041 aagctaattt gggatgttgg ccaccaggcc tacgcacata aaattcttac gggcagaagg
     2101 gatgttttca acaccaatcg caggtataaa ggaataagcg gtttcccaaa acggagcgag
     2161 agcccctacg atgcatttgg cgtt
//
LOCUS       DAOOQX010000339         2183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_62011_length_2183_cov_3.289474, whole genome shotgun sequence.
ACCESSION   DAOOQX010000339 DAOOQX010000000
VERSION     DAOOQX010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62011_length_2183_cov_3.289474"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PLX53_06105"
     CDS             <1..329
                     /locus_tag="PLX53_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOY72691.1"
                     /translation="PSVQRVMMGYNFPGNIRELRNMVERAIILCDGSKLSLKNFPGLT
                     SQTGEAGTQLPGIGDDVFDLDLIEKFVIMKALERTGYKKTEAAQLLNITRQSLDRRLE
                     KYSINI"
     gene            complement(326..826)
                     /locus_tag="PLX53_06110"
     CDS             complement(326..826)
                     /locus_tag="PLX53_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOY72692.1"
                     /translation="MVELVKGDITLMDVDAIVNAANESLLGGGGVDGAIHRAAGPQLL
                     AECRTLGGCPTGEARITRGYNLKARYIIHTVGPVWHGGSRGEAKLLASAYWNSLQLAA
                     DYSLKSIAFPNISTGVYGYPKREAAEIATRTVNEFKQQNLHPERVVFVCFDDDSYQIY
                     KEMLKR"
     gene            1097..>2183
                     /locus_tag="PLX53_06115"
     CDS             1097..>2183
                     /locus_tag="PLX53_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOY72693.1"
                     /translation="MLRKSVYALALGGLLLTACQKEENFSDETNPLSTKQELKNGDII
                     PGKFIVVFTQDTFSTRVDKVQQVSYEESHAKALAEASTFLSVYAVPSGKIDQVYGASI
                     KGFSAELNELQVNAIYRDSRVAYIEPDRWIVLDYKVESISDPTAKGQTTPWGITRVGG
                     ATNYTGTNVAWIIDTGIDLDHPDLNVDVSRSKTFVPRGRTPDDDNGHGSHVAGTVAAK
                     NNTIGVVGVAAGAPVVAVKVLDRSGSGAYSTIISGVNYVASAGKSGDVANMSLGGPTY
                     QALDDAVLAASNKGIKFSLAAGNESANANLSSPARVNGSNIFTISAMDNADKFAYFSN
                     YGNPPIDYCAPGVSIYSTYKSGKYATLS"
BASE COUNT          609 a          506 c          514 g          554 t
ORIGIN      
        1 acccttcggt gcaaagggta atgatgggct acaatttccc cggaaacatc agggagctgc
       61 gcaacatggt tgagcgtgcc attattcttt gcgatggtag caagcttagc ctgaaaaatt
      121 ttccgggctt aacaagccaa accggagaag cgggcaccca actacccggc ataggcgatg
      181 acgtattcga cctggacctc attgaaaaat ttgtgattat gaaggccctg gaacgcacag
      241 gctacaagaa aacagaggct gcacagctgc tcaacatcac ccgtcaatcg ctcgaccgaa
      301 ggctggagaa atactccatc aacatttaac gcttaagcat ctccttgtat atctgatagc
      361 tatcgtcatc aaagcagaca aaaacaaccc tctcgggatg taggttttgc tgtttgaatt
      421 cgtttacggt tctagtggct atttcggcgg cctccctttt gggataaccg taaacaccgg
      481 tgctaatgtt aggaaatgca atacttttca gcgagtaatc agctgcaagc tgtaagctgt
      541 tccaataggc tgaggccaga agcttagcct caccccgcga tccgccatgc caaacagggc
      601 ctacagtatg aattatgtac ctagctttaa gattgtagcc tctagtgatg cgtgcctcac
      661 cagtagggca tccacccaac gttcggcatt ctgcaagtag ctgcggtccc gcagcacgat
      721 ggatagcgcc atcaactcct ccaccgccaa gcaacgattc gttggcggca tttactatcg
      781 catccacatc cattaacgta atgtctcctt taaccagctc taccataacc tgttatcgtt
      841 ttaagtaaaa tggattttag atggttaaaa gatagtgaaa ttgttcttac aggtaacccg
      901 ggtgtgctaa aaaaaattga gttgaaagcc gtgagccggt aggcgttaaa ggtttaaagc
      961 tatgtgattg gctgcgtatt atttgaaatt taaatccgtc aaatgccgaa aaaaatagtt
     1021 aaaactttct gagcacttga cttttatgta atatagccat aatttgcacc tgaatactaa
     1081 cctaaaccat ttaaccatgt taagaaaatc tgtttatgct ctggcgcttg gcgggttgct
     1141 actgactgcc tgccaaaagg aagaaaattt ttcggatgaa accaacccgc tctcaacgaa
     1201 gcaggagctg aagaatggcg atatcatccc ggggaaattt attgtggtat ttacacagga
     1261 tacattcagc acaagggttg acaaggtgca acaggtaagt tatgaagaaa gccatgctaa
     1321 agcgctggct gaagcatcaa cctttttgag tgtttatgct gtaccatccg gtaagattga
     1381 ccaggtgtat ggagcttcca taaaaggttt ttccgccgaa ctcaacgagc tacaggtaaa
     1441 tgcgatttat agggattcga gagttgccta tatagaacct gatcgctgga tagtgctgga
     1501 ctacaaggtt gagtcaatct ccgatcccac agcgaagggc caaaccactc catggggtat
     1561 aacccgtgtt ggaggcgcta ccaactatac aggaaccaac gtggcatgga taatcgatac
     1621 aggtattgat ttagaccatc ccgatttaaa tgtggatgtt tctcgcagca agacattcgt
     1681 gccccgtgga agaactcccg atgacgataa cgggcatggc tcgcatgttg cagggacagt
     1741 tgctgcaaaa aataacacca ttggcgttgt tggggttgct gcaggagcgc ctgtagtggc
     1801 tgtaaaggtt ctcgaccgta gtggaagcgg agcttactcc accattattt ccggtgttaa
     1861 ctatgttgcc tctgctggca aatcgggtga tgtagccaac atgagcctag gaggccccac
     1921 ctatcaggca ctcgacgatg ctgtgctggc agcatctaac aaggggatta agttttcgtt
     1981 agccgctggt aatgaaagtg ctaatgccaa ccttagctct cctgcacggg tgaatgggtc
     2041 taacatcttc accatctcag caatggataa tgccgataaa tttgcatact tctccaacta
     2101 tgggaatccg cctatagact actgtgctcc cggagtaagc atctactcga catacaagtc
     2161 gggtaaatac gcaaccctta gcg
//
LOCUS       DAOOQX010000340         2176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_62279_length_2176_cov_3.647336, whole genome shotgun sequence.
ACCESSION   DAOOQX010000340 DAOOQX010000000
VERSION     DAOOQX010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62279_length_2176_cov_3.647336"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PLX53_06120"
     CDS             <1..355
                     /locus_tag="PLX53_06120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOY72694.1"
                     /translation="PPVKGIVTNRYSPLQNHYGIDLVAAPNEVVLAVAKGTVILADWT
                     IETGYTIAVQHENNFISVYKHNSKLLKRQGDVVGAGDAIAIIGNSGELTTGPHLHFEL
                     WQNGNTLDPSKFIHF"
     gene            368..1525
                     /locus_tag="PLX53_06125"
     CDS             368..1525
                     /locus_tag="PLX53_06125"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOY72695.1"
                     /translation="MKNVAILGSTGSIGTQALQVIESNPELFSVELLTAQNNWKLLVS
                     QALKYQPNAVVIGNDKYYVNVKSALEKTDIKIFAGKNSIADCVGSAQVDMVLVALVGF
                     AGLEPTISAIKAGKTIALANKETLVVAGELISELAMEHKASVLPVDSEHSAIFQSIVG
                     ERSPIEKIILTASGGPFRNFSLEQLGRVTPSDALQHPNWCMGNKITVDSASLMNKGLE
                     VIEARWLFNLKPSQIEVVVHPQSIVHSMVQFEDGSVKAQLGLPDMRIPIQYALTYPFR
                     VKSDFPRINFGNNLQLTFERPDTVRFPNLALAREALERGGNMPCVLNAANEVAVEGFL
                     EQSIGFMQMSEIIAEVMSKSSYIAYPSLDDYKMTDIETRLKTAELISQNLA"
     gene            1525..>2176
                     /locus_tag="PLX53_06130"
     CDS             1525..>2176
                     /locus_tag="PLX53_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOY72696.1"
                     /translation="MEILVKVGQFFLSLSILVILHEMGHFMFAKLFKTRVEKFYLFFN
                     PWFSLFKFKHGETEYGIGWLPLGGYVKISGMIDESLDKEQMKQPPQPYEFRSKPAWQR
                     FLIMIAGVMVNVVLAIVIYCATLYTWGEEYLPTRSLKYGVSCDSLAQSIGFQNGDKIL
                     SVSGREVENFQMVAHDILLNDDRTVEVLRGNDTIKITVGEEALASLVKNPLIFIPRI"
BASE COUNT          568 a          466 c          538 g          604 t
ORIGIN      
        1 acccccagta aagggtattg ttaccaaccg ttacagtcct ctgcagaacc attacgggat
       61 agatttagtg gctgctccta atgaggtggt tctggcggta gccaagggga ctgtaatttt
      121 ggccgattgg actattgaaa ccggctatac tattgctgtt cagcatgaaa ataactttat
      181 atcggtttac aagcacaaca gcaagctgct taagcggcag ggcgatgtgg taggtgcagg
      241 cgatgccatt gccataattg gtaactcggg cgagttgacc accggccccc acttacactt
      301 tgagctgtgg caaaatggta acacgctcga tccttcaaag tttatccatt tttaaggagc
      361 tgaaccgatg aagaatgttg ctattcttgg ttctacaggc tccattggaa cccaggcgtt
      421 gcaggttatt gaatctaatc cggagctttt ttctgttgaa ttgcttacag ctcaaaataa
      481 ctggaaactt cttgtttcac aggctctaaa gtatcaacct aatgccgtag taattggtaa
      541 tgataagtac tacgtaaatg ttaagagcgc tctcgaaaag acggatatta agatttttgc
      601 ggggaaaaac tctattgcag attgtgttgg gtcggcacaa gttgacatgg tgcttgtggc
      661 tcttgtaggc tttgctggat tggaacctac aatttcagcc ataaaagctg gtaaaacaat
      721 tgccctagcc aataaggaaa ctctggtggt ggcaggggag ctgatttctg aattggccat
      781 ggagcataaa gcatcagttt taccagtcga ttcggagcat tcggctattt ttcagtcaat
      841 tgtgggtgaa cgtagcccaa tcgagaaaat tatactcacc gcttcaggtg gcccattcag
      901 gaatttctcc ctggagcagc ttgggagggt tactccaagc gatgcgctgc agcatccaaa
      961 ctggtgcatg ggcaacaaga tcactgttga ttcagcctca ctgatgaata aggggcttga
     1021 ggtgattgag gccaggtggc tcttcaacct aaaacccagc cagattgaag tggtagttca
     1081 tcctcaatca attgtccact ccatggttca gtttgaggat ggctcggtga aagctcagct
     1141 cgggttgccc gatatgcgaa tccctataca gtatgccctt acctatcctt ttcgggttaa
     1201 atcggacttc ccccggatta actttggcaa caaccttcag ctgacttttg agcgtcccga
     1261 tactgttcgt tttcctaatt tggcgctggc cagggaggct ttggaacgtg gaggtaatat
     1321 gccttgtgtg ttaaatgctg ctaacgaggt ggccgtggag ggtttccttg agcagagcat
     1381 cggtttcatg cagatgtccg aaataattgc tgaagttatg tcaaaatcta gctatattgc
     1441 atatccatcg cttgacgatt acaagatgac agatattgaa acccgcttga aaacggcaga
     1501 actaatctca caaaaccttg cataatggaa atactggtta aagttggaca attcttttta
     1561 agcctttcaa ttttagtcat tctccacgag atggggcatt ttatgtttgc caagctcttc
     1621 aaaacccgtg tggagaagtt ctacctgttt tttaacccat ggttctcgct ttttaagttt
     1681 aagcacggcg aaacggagta tggaataggt tggctaccat tgggtggata cgttaagatc
     1741 tcggggatga ttgatgagtc gctagacaag gagcagatga agcagccgcc tcaaccctac
     1801 gagttccgtt caaaacccgc ctggcagcgt tttttgataa tgatcgcagg agtaatggtg
     1861 aacgtagttc tggccattgt gatttactgc gctacgctct atacctgggg cgaggagtat
     1921 cttccaacac gaagcctgaa gtacggcgtc agctgtgatt cccttgctca aagcattggc
     1981 tttcagaacg gcgacaaaat tctttcggta agcgggagag aagttgaaaa tttccagatg
     2041 gttgcccacg atattctcct caacgacgac cgaacggtag aggttttacg tggaaacgac
     2101 accataaaaa ttacagttgg ggaggaagcg ctggccagcc tggtaaagaa ccccctgatt
     2161 ttcataccac gtattc
//
LOCUS       DAOOQX010000341         2166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_62745_length_2166_cov_2.440076, whole genome shotgun sequence.
ACCESSION   DAOOQX010000341 DAOOQX010000000
VERSION     DAOOQX010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62745_length_2166_cov_2.440076"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="PLX53_06135"
     CDS             <1..178
                     /locus_tag="PLX53_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOY72697.1"
                     /translation="CVTYKGTVDQVSVLRGVDPTLDEEAIRVIKMLPQWKPGKQGGKP
                     VNVWYTVPISFQLK"
     gene            320..622
                     /locus_tag="PLX53_06140"
     CDS             320..622
                     /locus_tag="PLX53_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72698.1"
                     /translation="MAIRITELFGAKAGDDWGRHYMGIIKHCVLPNGQQGVFGRIDIK
                     DSEHNEVACASANSREQLGVKLDLLVLMVLDYGILDLKANGMNSDTFGTNMTIGLS"
     gene            1331..>2166
                     /locus_tag="PLX53_06145"
     CDS             1331..>2166
                     /locus_tag="PLX53_06145"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOY72699.1"
                     /translation="MRRILIVVAMILSYHILNAQNKITGKISDQANLPLIGVSVFVPD
                     MSKGTTSGTNGSYELSGLPNGKIKIQFSLLGYAGRIETVVLSGEGLELNIVLNQTALE
                     LEEIVVSGGYSSTQHENAVKIDVLKLDPRTGTTTPNFTETLTKVPGVDMISKGSGVSK
                     PVIRGLSMNDILVLNNGVRFENYQYSSHHPLGIDEFGVEDVEVIKGPASLLYGSDAIG
                     GVINFIKEKPAPIGSITGDYNMQLFSNTLGATNNFGIKGASNKFFGGLRVGQKTNSDF
                     LQG"
BASE COUNT          637 a          406 c          473 g          650 t
ORIGIN      
        1 ctgcgttacc tacaagggta ctgttgacca agtgtcggtt ctccgtggcg ttgacccaac
       61 cctcgacgag gaggccattc gggttataaa gatgctaccc cagtggaaac ccggtaagca
      121 gggcggtaag ccggttaacg tttggtacac cgttcctatc agcttccagc tgaagtagga
      181 gtaatatagt tttttgtgaa aaaggcgctt caaccgatgc gcctttttca tttacccctt
      241 gccatttttt tcgtgaatat ttttgaaaac atttcaacag gtgcatacgt aaaaaatgaa
      301 cctaaaaaac atcgggagta tggctattcg aattaccgag ctatttgggg caaaggccgg
      361 ggatgattgg ggcaggcatt acatgggcat catcaaacat tgcgtgctac ctaatggaca
      421 gcagggtgta ttcggccgaa ttgatattaa ggatagcgag cataacgagg tagcttgcgc
      481 tagtgccaat agccgtgaac agctgggtgt aaagctcgat ttgcttgttt tgatggtgct
      541 ggattatggg atactcgatt tgaaggcgaa tggcatgaat agcgatacct ttggcaccaa
      601 catgaccatt gggctaagct gagagctggg ttgtgattta ctccacgatt ttgtcctttt
      661 aaaagttttt tttgttaaaa agcggcggtt gaaatccgtg aatgttcgct atttttcttt
      721 tcctgtcaac aaaagcaatt tactgttgtt tacattaacg ttgaagtatt gcccaggctc
      781 tacggccagg cagttaatcc tttctatttc tttggattaa gacgacaggg taatacattt
      841 aagcggttgg gataaggggg aaaggagttt tttcccctct ttgttttaaa aaatatattt
      901 atcctacttc ccatccctcc taaaaggctg caccagctac ttgaacttag atgacaaaaa
      961 gacctatcaa tattatttca atagcattgg tgcttatttt tatagcgcca atgtcaatta
     1021 agcttgttga cggacttttc catcaccatg accattttca ttgcaccgca acaaacgaga
     1081 agcattttca tgtatatcac gaaaaatgtc ccataccggg ttttgagcta tctctttttt
     1141 cggctgaaaa gcatttacaa atctctcaga aacacaatta ctgtgtaaaa ccaaatgacg
     1201 attacaactt tgtttattgt agcaataatt cagagtactc atttttacta cgtgcaccac
     1261 catgtttaca ggtgtgaaat gtacatctgt taaggtataa aagtttgttt ataagctaaa
     1321 acgtaataaa atgagaagaa tacttatcgt tgttgctatg atattgtcgt atcatatatt
     1381 gaacgcccaa aacaaaataa cagggaaaat ttcagatcaa gctaatttac cgttgattgg
     1441 tgtaagcgtt tttgttcccg atatgagtaa gggtacaaca tcgggaacta acggatctta
     1501 tgagctgtca ggtttaccga atggtaaaat taaaattcaa ttctccttgt tgggctatgc
     1561 cggtagaatt gagaccgttg tgttatctgg agaagggttg gagttgaaca tagtgctaaa
     1621 ccaaacagct ttagaattgg aggaaatagt tgtttccggt gggtatagct cgacccaaca
     1681 cgaaaacgct gtaaaaattg atgttctcaa actcgaccca cgaacaggta caactacgcc
     1741 aaattttacg gaaacactca caaaagttcc cggcgttgat atgatttcta aaggaagtgg
     1801 tgtatcgaaa ccggttatca gaggactctc gatgaatgat attttagttc tgaataatgg
     1861 ggtgagattt gagaactacc agtattccag ccatcatccc ctcgggatag acgagtttgg
     1921 agttgaagat gttgaagtaa ttaaaggccc tgcttcattg ctttatggct ccgatgcaat
     1981 cggtggtgtt atcaacttca taaaagaaaa acccgcaccc ataggtagca ttactggcga
     2041 ttacaacatg caactttttt caaacacgct gggtgcaact aataattttg gtataaaagg
     2101 agcttcaaat aagttttttg gagggttaag agttggtcaa aaaacaaact ccgattttct
     2161 tcaagg
//
LOCUS       DAOOQX010000342         2163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_62846_length_2163_cov_3.361480, whole genome shotgun sequence.
ACCESSION   DAOOQX010000342 DAOOQX010000000
VERSION     DAOOQX010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62846_length_2163_cov_3.361480"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLX53_06150"
     CDS             complement(<1..299)
                     /locus_tag="PLX53_06150"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOY72700.1"
                     /translation="MNLIIDVGNTQVKVALMEQGEIVFLKSFSTLSAGSMEEILSTYL
                     PHKAIVSVVGNFQPEILSYLSQKLMVHVLGSKSKLPLKNTYETPLTLGYDRIAVAV"
     gene            complement(340..>2163)
                     /locus_tag="PLX53_06155"
     CDS             complement(340..>2163)
                     /locus_tag="PLX53_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008021812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOY72701.1"
                     /translation="KRKIKDIARELIALYSQRRSQPGFSFSPDTYMQQELEASFVFED
                     TPDQLSATKAVKEDMEAERPMDRLICGDVGFGKTEVAIRAAFKAVADSKQVAVLVPTT
                     ILALQHYQTFTSRLKGFPCTVEFLNRMKTPAQQREIRQRASEGKIDILIGTHALLNST
                     LKFKDLGLLVVDEEQKFGVAAKEKLKQLKLNVDTLTLTATPIPRTLQFSLMGARDLSI
                     INTPPANRHPILTEVHTFSTAIIKEAIDYEMDRGGQVFFVHNRIQNIAEVELMVNKIC
                     PKAKTAVAHGQMEPSKLEKIMLGFISGDYDVLISTSIIESGLDISNANTMIINNAQMF
                     GLSDLHQLRGRVGRSNKKAFCYLLAPPLETLTADARRRLRAIEEFSELGSGFSIAMQD
                     LDIRGAGNILGAEQSGFITEIGFEAYQRILDEALEELRESEFVTIQTQAEHETQREWK
                     PSYADCHVETDMEILLPDSYITNTTERIKLYREIDSLHNAEELERFRNEIRDRFGPLP
                     RQVEDLLSVVRLRWIAVELGFEKLILGNGQLMAHFISNQLSPYYRSSTFEGIIRFIQQ
                     HPERFKLKEGKDKLTIRIAGVGEISEAIETLQKLHETLPVK"
BASE COUNT          536 a          520 c          501 g          606 t
ORIGIN      
        1 acggcaaccg caatccggtc gtaaccgaga gtcagcgggg tttcataggt gttttttaaa
       61 ggaagttttg acttagaccc aagcacgtgt accataagtt tttggctgag gtagcttaat
      121 atttcgggct gaaaatttcc aacaaccgag actatagcct tatggggcag gtaggtagat
      181 aaaatttctt ccatgctccc agcgctgagg gtggagaaac ttttcagaaa gacaatctcg
      241 ccctgctcca tgagggcaac ctttacctgc gtatttccaa catcaataat taagttcata
      301 cataacagga atatattggc ggatgtgtaa atttatgggt cattttacgg gcaaggtttc
      361 atgaagcttc tgcaacgttt caattgcctc actaatttca cctactccgg ctatacggat
      421 tgtcagctta tcctttccct ccttgagttt aaagcgctcg ggatgttgct ggatgaaacg
      481 aattataccc tcaaaagtag aagaacggta gtacggcgaa agctggttgg agatgaaatg
      541 agccatcagc tggccgttac ccagtattag cttttcaaag cccagctcaa cggctatcca
      601 cctaagtctt acaacgctta agagatcttc cacctgccgg ggcagtggtc caaacctatc
      661 ccgaatttca ttcctgaacc tttctagctc ctcggcatta tggaggctat caatttcacg
      721 gtagagctta atccgctcgg tggtgttggt gatgtagctg tcgggtagaa gtatttccat
      781 gtcggtttcc acgtggcaat cggcatagga aggcttccat tccctttggg tctcatgttc
      841 ggcctgagtt tgaatggtaa caaactccga ttccctcaac tcctcgagtg cttcatccaa
      901 aatacgctgg taggcctcaa aaccgatttc ggttataaac ccgctttgct ccgccccaag
      961 aatgttacca gctcctcgta tatccaaatc ctgcatggca atgctaaatc cactgccgag
     1021 ttcggaaaac tcctcaatag ccctcaacct acgacgagca tctgctgtta aagtttccaa
     1081 cggtggggca agcaggtagc aaaaggcctt tttatttgaa cgccctaccc tgcccctaag
     1141 ctggtgcaaa tcgcttagcc caaacatctg ggcattgtta ataatcatgg tattggcatt
     1201 cgaaatatcc aatcctgact caattataga ggttgatata agcacatcgt agtcaccgct
     1261 tatgaagcct agcataattt tctctagttt cgagggctcc atctggccgt gggctacagc
     1321 ggttttggct tttgggcaaa tcttattcac catgagctcc acttcggcta tattttgaat
     1381 acggttatgc acaaagaaca cctgtccgcc cctatccatt tcataatcaa tggcttcctt
     1441 tataattgct gtgctgaagg tatgcacctc ggttagtatg gggtggcggt tggcaggcgg
     1501 agtattgatt atggagagat cgcgagcacc catgagtgaa aattgaaggg tgcgtggaat
     1561 tggagtagcg gttagggtga gggtatcaac attcagcttt agctgcttaa gcttttcctt
     1621 tgctgcaaca ccgaattttt gctcctcatc cacaacgagt aaccccaaat ctttaaactt
     1681 cagcgtgctg ttgagtaggg catgggttcc aatcagaata tcaattttgc cctcggatgc
     1741 cctttggcgt atctcgcgtt gctgtgcggg agttttcatg cgattgagaa actcaactgt
     1801 acaggggaac cccttgagcc tactggtgaa tgtttggtaa tgctgaaggg caagaatggt
     1861 ggttggcacc agcacggcaa cctgcttgct atcggcaacc gctttaaagg ctgccctaat
     1921 ggcaacttct gtttttccaa atcctacatc accgcatatc agcctatcca ttggcctttc
     1981 agcctccatg tcttccttaa ctgccttagt ggcagaaagc tggtcgggag tatcctcaaa
     2041 aacaaaagag gcttcaagct cctgctgcat gtaggtgtcg ggcgaaaagc tgaaaccggg
     2101 ctgcgacctt cgctgggagt acagggcaat caactcccgg gcaatatcct tgatttttct
     2161 ttt
//
LOCUS       DAOOQX010000343         2152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_63323_length_2152_cov_2.876967, whole genome shotgun sequence.
ACCESSION   DAOOQX010000343 DAOOQX010000000
VERSION     DAOOQX010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63323_length_2152_cov_2.876967"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PLX53_06160"
     CDS             complement(<1..969)
                     /locus_tag="PLX53_06160"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY72702.1"
                     /translation="MAKDIILELSPENAANSQLLLKAVASKLGMSVKDVGHYTITRRS
                     IDSRHGKVRVNLGIRVFDRDEPIADGKPQFTYGDVSKSPPVAVIGAGPAGLFAALRLI
                     EHGYKPIVVERGKEVSARKRDVALLNREHKLNPDSNYAFGEGGAGTFSDGKLYTRSRK
                     RGDYNRVLYILCNHGASEDILFDSHPHIGTNRLPGIIVKIRETIISYGGEILYNSRVE
                     SIKTDGSRVTGVKLSDGRLVEAKACILATGHSARDVYSMLLNQQIQIEAKPFAMGVRV
                     EHPQAIIDSIQYHGTPRGDYLPAASYSLVQQVEGRGVYSFCMCPGGF"
     gene            complement(1165..1905)
                     /locus_tag="PLX53_06165"
     CDS             complement(1165..1905)
                     /locus_tag="PLX53_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOY72703.1"
                     /translation="MKKIFVVIAVIWCSVTAAQAQYRKPNILNDPDYDVGKPLRFGFS
                     IGINIMDFDIKNSSVPELNADSSQFQYFTEVTHINPGLNVNAISDLRLAENFHLRFLP
                     GYSFGQRNLDFFKVENGRVTHETTMDIESNFIDLPLGIKYLAERTSNIRPYLYVGGDY
                     RIDLAAYKRLKVEKGILVRLQKQDFYYELGFGVDFFFEYFKFSTEVKWSAGIGNVISS
                     DYAEGAENYRYAVDAMNSRLIMISFHFE"
BASE COUNT          617 a          515 c          460 g          560 t
ORIGIN      
        1 gaaccctccc gggcacatgc agaacgagta aacacccctt ccttcgacct gctgcacaag
       61 cgagtaggat gctgctggca agtagtcgcc ccgaggagta ccatggtact gtatggagtc
      121 aataatagcc tgaggatgct ccacccgaac acccatggca aagggtttcg cttctatctg
      181 aatttgctga ttcaacagca tggaatacac gtcgcgagcg gaatgcccgg ttgccagtat
      241 gcaggccttg gcctccacaa gtcggccatc ggaaagttta acacccgtaa ccctgctccc
      301 gtcggtttta atactttcaa cccgggagtt atatagaatt tcgcccccgt agctaatgat
      361 tgtctccctg attttcacaa taatgccggg caagcgattt gttccaatgt gggggtgcga
      421 atcgaaaagt atatcctcgc tagccccgtg gttacaaagg atgtacaaca cacgattgta
      481 atcacctctt tttcgcgaac gggtgtagag tttcccatca gaaaaggttc ctgcacctcc
      541 ttcgccaaaa gcatagttgg aatcggggtt gagcttatgc tcccggttta gcagggcaac
      601 atcccttttg cgggcgctta cttctttgcc ccgctctacc actattggct tgtagccgtg
      661 ttcaataagc cttaatgcgg caaacaaccc agcaggacct gcgccaataa ctgcaacagg
      721 aggcgatttg gaaacatcgc cataggtgaa ttggggttta ccgtcagcaa taggttcatc
      781 cctatcaaaa accctgatgc caaggtttac ccttaccttt ccgtgtcgtg agtctattga
      841 ccgtcgggta atagtgtaat ggccgacatc tttaacactc attcccagct tactggcaac
      901 tgctttcagc aatagctggc tgttggccgc attctcaggg ctgagttcaa gaattatatc
      961 cttagccata atgaaaaatt atcagcatca gtgtgaaaaa aaggaatttc tccaaaaaat
     1021 agtgttaagc cccttctcaa taatccctaa aacccggcga ggtgaacaat ttatagctga
     1081 ttgagggtat atcaaaattc cgcgtattac cccaactaca agaagatgga tgaacagcct
     1141 gttttatgct acacttccac ttttctactc aaaatggaag gaaatcatta tcagcctgga
     1201 gttcatagca tcaacagcat agcggtagtt ctcggccccc tcggcatagt cgcttgaaat
     1261 aacgttgcca atgcctgccg accatttcac ctctgttgaa aacttaaaat attcaaaaaa
     1321 gaagtcaaca ccaaagccga gctcgtagta gaaatcctgc ttttgcagcc taaccagtat
     1381 tcccttttcg accttaagcc gtttataggc agctaaatca atacggtaat cgccacccac
     1441 gtatagatac ggcctaatgt tgctggttcg ctcggcaagg tacttaatgc ctaaaggtaa
     1501 atcaataaaa ttcgactcaa tatccattgt agtttcatgg gtaacccgtc cattttcgac
     1561 cttaaaaaaa tcgagatttc gctgcccaaa ggagtaaccc ggtaaaaagc gtaggtgaaa
     1621 attttcggct agccttaaat cggaaatagc gttgacattt aaacccgggt ttatatgcgt
     1681 aacctctgtg aagtactgga actgggagct gtcggcatta agctcgggaa cggagctgtt
     1741 cttaatatca aaatccataa tgttgatgcc gatggagaaa ccaaacctta ggggtttgcc
     1801 aacgtcataa tcgggatcgt tgagaatgtt aggcttacgg tactgcgcct gagctgcagt
     1861 caccgagcac cagataaccg ctattacaac aaagattttc ttcaaaatag ttataaactc
     1921 ttagggtaaa acatctacgt aaaaaaatta ccttggatga atgactctca aacggatact
     1981 gcacctgaac atttatgtac tataggtaaa gcctgttatc aaacgtgcag ggtataaaaa
     2041 atattgcaaa caataaccta aacagacgtc aaacattcgt ggtagggttt ctaatggtcg
     2101 gttgttgagt aagcaatcag ggcttctccc ctgaaatggc ttcatgcaca aa
//
LOCUS       DAOOQX010000344         2152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_63331_length_2152_cov_2.484502, whole genome shotgun sequence.
ACCESSION   DAOOQX010000344 DAOOQX010000000
VERSION     DAOOQX010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63331_length_2152_cov_2.484502"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1930
                     /locus_tag="PLX53_06170"
     CDS             <1..1930
                     /locus_tag="PLX53_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72704.1"
                     /translation="ANSPPEVLYHLGISYMHQNNFDKAKAQFEAYKKYVNPNDAYVLQ
                     QVEQNIKSCETAKAMMDSPIGLNLNRLEEGVNNDQSNFDAVLSGDGKTMAFTSRSTSG
                     NKVFISFMDKGKWSIPKDITRNLGSSYLTTSFLSYNGEELYLIDNDLKNSEIVVSFLQ
                     KNKWSNPVKAAKPINSKANETHVCVTRDGNTAYFTSDRKGGQGGFDIYKVTISGDKWG
                     EPVNLGPNINTPLDEATPFLTPDEKYLFFSSQGHNSMGGYDIFYVNLEGSSNVKNIGY
                     PLNTPDDNLFYFPTSLTSGYISTFSPNGLGGLDICSVEIIPQVDVKINIMLADKAPAD
                     KSYTVSIIDTKDGKPVSSVNQNGKKQILQKLSPGTFTVAASGVEFDSTSSLLEIPSKP
                     DKNEYQISLLLNPSQPKPEPVAEVSQPANDIPIQKEVKPVPVVVEEPKPEKPVPASVE
                     KPKQEKSTAASVPVNPSPKPEVKKREMEKPKPVHVAVTNLSLAAGTSTSLAHPTYSVQ
                     LMACKQPVDYDYFKLDSIFVSISPEGYYRYSVGYTLNVQQAEELLARVKTLGYTNAYV
                     RINKEQPSYTIQLMALTKPKRLNQLNVIGSTMVYRGCDGIYRYCTGSYSTPEEAQNDL
                     ASFKNAGYSDAFIRHLGR"
     gene            2048..>2152
                     /locus_tag="PLX53_06175"
     CDS             2048..>2152
                     /locus_tag="PLX53_06175"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOY72705.1"
                     /translation="MPAEKTEMERVVVGLSGGVDSSVAAYLLKEQGYEV"
BASE COUNT          671 a          544 c          444 g          493 t
ORIGIN      
        1 ggccaactca ccccctgaag ttctctacca cctgggcatc tcctacatgc atcaaaataa
       61 ttttgataaa gccaaagctc agtttgaagc ctataaaaag tacgtcaacc caaatgatgc
      121 gtatgttttg cagcaggttg aacaaaacat aaagagctgt gaaacagcca aggctatgat
      181 ggacagccca attgggctaa acctaaaccg acttgaggaa ggtgtaaaca acgaccagtc
      241 gaacttcgat gccgttcttt cgggcgatgg aaaaacgatg gccttcacca gccgatccac
      301 ctcgggtaac aaggtgttca tctccttcat ggataagggt aaatggtcaa tcccaaagga
      361 tattacccgg aaccttggta gcagctacct caccacctca ttcctatctt acaatggcga
      421 agagctctac ctgatagata acgatttgaa gaattcagaa attgtagtta gctttttaca
      481 aaaaaataaa tggtcgaacc cggtaaaggc tgcaaaacca attaactcta aggctaatga
      541 gacccatgtt tgcgtaaccc gcgatggcaa caccgcatac ttcaccagcg acagaaaagg
      601 ggggcaagga ggatttgaca tctacaaggt aaccattagc ggcgacaaat ggggcgaacc
      661 cgtaaacctt ggtcccaaca tcaacacccc tttagatgaa gcaacaccat tccttacccc
      721 cgatgagaag tacctgttct tcagctccca ggggcacaac agcatgggtg gatacgatat
      781 cttttatgtg aacctggaag gaagctctaa cgtcaagaat attgggtacc ccctaaacac
      841 ccccgatgac aaccttttct acttcccaac cagcctgacc agcggctata tttcaacatt
      901 ttctcccaat gggctgggcg gtctggatat ttgctcggta gaaatcattc cccaggttga
      961 tgttaaaatc aacataatgc ttgccgacaa ggcaccagcc gataaaagct acaccgttag
     1021 catcattgat acgaaagatg gcaagccggt tagcagtgta aaccaaaacg ggaaaaaaca
     1081 aattttacaa aaattatccc cgggaacgtt tactgttgct gcttcgggtg tagaatttga
     1141 ctctacttcg agccttctcg aaattccctc aaaacccgac aaaaacgaat accagattag
     1201 cctattgctg aacccctccc aacctaaacc cgaacctgtt gcagaggtat cccaaccggc
     1261 aaatgatata ccgattcaaa aggaagtaaa gcccgtacca gtagtggttg aagagcctaa
     1321 gcccgaaaag cctgttcctg cttcggtaga gaagccaaag caggaaaaat caacagctgc
     1381 cagtgtacct gttaacccat ctcccaagcc tgaagttaaa aaacgtgaga tggaaaagcc
     1441 caagccagtt catgttgccg ttacaaacct ttcattagca gctggaacct ctacatcatt
     1501 agcccaccct acctattccg tacagcttat ggcttgcaag cagcctgtgg actacgacta
     1561 ctttaaactc gacagcatat ttgtttccat atcgcccgaa ggctactacc gttacagcgt
     1621 tggttatact ctcaacgtac agcaagccga agaactcctt gccagggtaa agacattagg
     1681 ctacaccaac gcctatgtta gaattaacaa ggagcagcca agctacacca ttcaacttat
     1741 ggcactcaca aaacccaaac ggttaaacca gctaaacgta attggtagca ctatggttta
     1801 ccgtggatgt gatggaatat atcgttactg caccggtagc tactctacgc ccgaagaggc
     1861 gcagaatgac ctggctagct ttaaaaatgc cggatactcc gatgccttca tcaggcattt
     1921 ggggagataa aattaacccg ttcagaaaaa attttccaac acagccaaac cgcttaaccc
     1981 tgaaaccgcc ctagtattat acattgccat ttggggcact gccaccaaac cgtacttatt
     2041 tgtaactttg ccagctgaaa aaacagaaat ggaacgtgta gttgttggac tttcgggagg
     2101 cgttgattca agcgtagcag catacctact taaggagcaa ggatatgagg tg
//
LOCUS       DAOOQX010000345         2151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_63377_length_2151_cov_2.732347, whole genome shotgun sequence.
ACCESSION   DAOOQX010000345 DAOOQX010000000
VERSION     DAOOQX010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63377_length_2151_cov_2.732347"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2151
                     /gene="glgP"
                     /locus_tag="PLX53_06180"
     CDS             <1..>2151
                     /gene="glgP"
                     /locus_tag="PLX53_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOY72706.1"
                     /translation="MNNAQEIASIALWKNFIDYYYKAYDKALSLVGSRTHQFMELERQ
                     EILPTVERKFRTTQPSWTRLIVQKNLPEKLKPLDELSRNLWWSWNTDSKKLFESIDKE
                     LWIKCEKNPILFLERISLPRFQELEHDAEFVEKLHSIYLNFTAYMMQRYQRKGPKIAY
                     FSMEFGLHSSLKLYSGGLGILAGDYLKEASDRNIDMVGVGLLYRYGYFTQQLSTSGQQ
                     IAVYDQQNFTQSLALPVRDEDGRWVTVSVAFPGRQVQARIWKVDVGRTELYLLDTDFE
                     ENLVNDRTITHYLYGGDLENRFMQEMILGIGGIRALNELGIKADIYHLNEGHAAFTGL
                     ERLRKFILDEKLSFSEAMEVVRATSLFTTHTPVPAGHDAFPEELVRTYMSHYPDRLMI
                     SWDQFMDLGRMVPGDSTQRFSMSVLATNLAQEVNGVSWLHGEVSRKMFAGMWPSYFPN
                     ELHISYVTNGVHFPTWTAREWKSIMENGQATNSNGYKTPAWNELENIPDSKIWEIRNT
                     LRKRLIKLINNRLSNPEMTRFESPRQVIEIQEKLSDSILTIGFARRFATYKRANLLFK
                     DLDRLDQLVNNPEHPVQFLFAGKAHPNDKAGQDLIKSIIEIAKQPRFLGRILFLQNYD
                     MELSRHMIQGTDVWLNTPTRPMEASGTSGQKAVMNGVLHFSVLDGWWVEGYRENAGWA
                     LPMESTYAESEFQDELDAEMIYTILENEIVPAFY"
BASE COUNT          608 a          547 c          505 g          491 t
ORIGIN      
        1 gatgaacaat gcgcaggaaa ttgcatctat tgccctttgg aaaaacttta ttgactacta
       61 ctataaagct tacgataaag ccctttcgct ggttggtagc cgaacccatc agttcatgga
      121 gctcgaaagg caggaaattc tgcctacagt tgagcgcaaa ttccgaacca cccaaccctc
      181 ttggacccgc ctgattgttc agaagaacct tcctgaaaag ctaaaacccc ttgacgagct
      241 ttcacgaaac ctttggtggt cgtggaatac cgattccaaa aaactatttg aatccatcga
      301 caaggaactc tggataaaat gtgagaaaaa ccccatacta ttcctcgaga gaattagcct
      361 accgcgtttt caggagctgg agcacgatgc tgaatttgtg gaaaagcttc actccatata
      421 cctgaatttt accgcctaca tgatgcagcg ctaccagcgc aaagggccta aaatagccta
      481 cttcagcatg gagtttggcc tccacagctc actgaagctc tactcgggtg ggttgggcat
      541 cttggccggc gactacctca aagaagcctc cgacaggaat attgacatgg taggtgttgg
      601 cctactttac cgttacggtt actttaccca gcagctatcc acctctggcc agcaaatagc
      661 cgtatacgac cagcaaaact tcacccaatc gctggcctta ccagttcgcg acgaggatgg
      721 cagatgggtt acagttagcg tggcattccc tggaaggcag gtgcaggcac gaatctggaa
      781 ggttgatgtg ggtagaacag agctttacct gttggacacc gacttcgagg agaacctcgt
      841 aaacgaccgg acaattactc actaccttta cggtggcgac ctggaaaaca ggtttatgca
      901 ggaaatgatt ctgggaattg gcggaatcag agcgctaaat gaactgggaa taaaggccga
      961 catttaccac cttaacgagg gtcatgcagc atttacagga ctggaaaggc ttagaaagtt
     1021 tattctcgat gaaaaacttt cattctccga agccatggag gttgtacggg ctaccagcct
     1081 gtttaccacc catacacccg ttccggccgg tcatgatgcc ttccccgaag aacttgtgcg
     1141 aacatacatg tcccactacc ccgacaggct gatgatttca tgggatcagt tcatggacct
     1201 gggtcgtatg gtacccggtg attccacaca aagattttcg atgagcgttc tggcaacaaa
     1261 cctggcccag gaggtaaatg gggttagctg gcttcacggt gaggtgagcc gaaaaatgtt
     1321 cgccggaatg tggccaagct acttccccaa cgagctacat atcagctacg ttaccaatgg
     1381 cgtccatttc ccaacatgga ccgcacggga gtggaaatca attatggaaa acggccaagc
     1441 cactaacagc aatggttaca aaactcctgc ctggaacgag ttggagaata ttccagattc
     1501 aaaaatttgg gaaattcgta ataccctcag gaaaaggctc atcaaactta tcaacaacag
     1561 gttgagtaac cccgaaatga cacgcttcga aagtccacgg caggtaattg agatacagga
     1621 aaaacttagc gatagcatcc tcaccattgg gtttgcccgc cgttttgcca cctacaagcg
     1681 cgccaacctc ctttttaaag atttggaccg gttggaccag ctggtgaata accccgaaca
     1741 tcctgttcaa ttcctctttg ccggaaaagc acaccctaac gataaggcag gacaagacct
     1801 cataaaaagc atcattgaga ttgccaagca accgcgcttc cttggacgaa ttctgttcct
     1861 gcaaaactac gacatggagc tctccaggca catgattcaa ggtaccgatg tatggctcaa
     1921 cacccctacc cgaccaatgg aggcttcggg taccagcggc caaaaagcag taatgaacgg
     1981 cgtgcttcac ttcagtgtcc tggatggctg gtgggttgaa ggttaccgcg aaaatgccgg
     2041 atgggcactt cccatggaga gcacctacgc cgaatccgag tttcaggatg aacttgatgc
     2101 cgaaatgata tacaccattc tggaaaacga aattgttccc gcattctacc a
//
LOCUS       DAOOQX010000346         2151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_63378_length_2151_cov_2.708015, whole genome shotgun sequence.
ACCESSION   DAOOQX010000346 DAOOQX010000000
VERSION     DAOOQX010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63378_length_2151_cov_2.708015"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /locus_tag="PLX53_06185"
     CDS             <1..1625
                     /locus_tag="PLX53_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOY72707.1"
                     /translation="SFRILLIIQLVGLSSAAKPQSKYFIGFTDKANSPYTISNPEQFL
                     SIRSIERRTRQNLALTESDLPVNPSYLDTLANMGITIHFPLRWMNGVVATISTPDQLT
                     QIQNIPFVSEIKEIYTPTKEHKSSKFTIEEPQSQPFSKPDKDAPNYGISATQVNMLNG
                     HILHGNGFLGEGIEIAVLDAGFYNVDSHPVFDSLWMNNRILGTKDFVNPSSNIFTEHH
                     HGMMVLSIMGGYANGQLVGTAPHARYWLIRTEDASSEQLVEEYNWAAGAEFADSAGAD
                     IINTSLGYTTFDVPSQNHTYSNLDGQTTVITRAANMAASKGMLVVVSAGNDGTSPWYY
                     ISAPADSPKVLTIGAVDGNRNKAAFSSFGPTADERVKPDLCAMGVGTIIATTDGLVGS
                     GNGTSFSAPLISGMMACLWQAKPNLTVSELMESVISASSSFNSPNNEIGYGIPDFALA
                     MPVGTYSYKPNIRIIPNPFSSQVQIYLPLEIDEAVSCQLLTPSGLTLYMCRQITENGV
                     LRINIPESLPKGLYIFHIAAGNQAFNLKAIKD"
     gene            1631..>2151
                     /locus_tag="PLX53_06190"
     CDS             1631..>2151
                     /locus_tag="PLX53_06190"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF025118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOY72708.1"
                     /translation="MSNGSTRLTLLELNLLVKETLAKSLAGSYWVVGEISEITEHPSG
                     HCYMELVQKGDDTDTIRAKARANIWGYTYRMLKPYFESTTNRPLSRGMKILVNAQVVF
                     HEYYGYSLTVLDIEPEYTLGDIELKRREVIARLISDGVMNMNKELELDMLPQRVAVIS
                     SANAAGFQDFVKQL"
BASE COUNT          603 a          539 c          502 g          507 t
ORIGIN      
        1 acagctttcg aatactacta atcatccagc tggttggttt atccagcgca gcaaaacctc
       61 aaagcaagta ctttattggc ttcaccgata aggccaactc cccatacacc atttcgaacc
      121 ccgaacagtt cctatctata aggtctattg agcgtagaac aaggcaaaat ttggcattaa
      181 cagaatccga tttacccgtt aaccccagct accttgatac gcttgcaaat atgggtatta
      241 caattcattt cccgcttcgg tggatgaacg gggttgtggc tacaatttca actcccgacc
      301 agctgacgca aattcagaat attccctttg tctccgaaat taaagagata tatacaccaa
      361 ccaaggagca taaaagctcg aaattcacaa tagaagaacc acaatcccaa ccattttcta
      421 aacctgacaa ggacgcacca aactacggga tatcggccac acaggtaaat atgcttaacg
      481 ggcatatact gcatggtaat ggctttttgg gagaagggat tgagattgct gttcttgacg
      541 ccgggtttta caacgttgac tcacatccgg ttttcgacag cctttggatg aacaaccgaa
      601 tactgggaac aaaggacttc gtcaatcctt cgtctaacat attcaccgaa catcatcatg
      661 gtatgatggt gctgtccatc atggggggat atgccaatgg acagcttgtt ggcacagcac
      721 ctcacgcccg ctactggctc atcaggactg aggatgccag ctccgaacag ctggttgaag
      781 agtacaactg ggcggcaggc gctgagtttg ccgatagcgc aggtgccgac atcattaaca
      841 cctccctggg atacaccact tttgatgtgc cttctcaaaa ccatacctac agcaaccttg
      901 acgggcaaac gacagtgatt acccgggcag ctaacatggc ggcatcaaag ggtatgctgg
      961 tggtggtgag tgccgggaac gatggtacat caccatggta ctacatttca gcaccagccg
     1021 actcgcctaa ggtgctaacc attggtgcag tggatggcaa taggaacaag gctgctttca
     1081 gctcctttgg cccaacagcc gatgaacgtg taaagccaga cttatgcgca atgggtgttg
     1141 gaacgattat tgctactacc gatgggctgg tgggcagcgg aaacggcacg tccttttccg
     1201 ctccactaat ctcgggcatg atggcttgcc tatggcaggc aaaacctaac ctaacagtca
     1261 gcgagctaat ggaatcggta atttcagcct caagttcatt caattcccct aataatgaaa
     1321 tcgggtacgg cattcctgac tttgccctgg ccatgccggt tggaacctac agctacaaac
     1381 caaacatcag aatcattcca aatccgttta gcagccaagt gcaaatatat cttccgctgg
     1441 agattgacga agctgtaagc tgccagctgc taaccccgag cggcctaacg ctctatatgt
     1501 gtaggcagat taccgaaaat ggggttttac gtattaacat tccggagagc ctaccaaaag
     1561 gcctctatat tttccatatc gctgccggta accaagcgtt caatttgaaa gctattaaag
     1621 actaaccccg atgagcaacg gcagtacccg cttaaccctt ctggagctaa acctgctggt
     1681 aaaagagact ttggccaagt cgcttgccgg tagctactgg gtggttggcg aaattagcga
     1741 gattaccgaa catccatccg gccactgcta catggaactt gtgcaaaagg gagatgacac
     1801 ggataccata agagcaaaag caagggcaaa catttggggc tacacctacc gaatgctgaa
     1861 accctacttt gagtcaacca ccaatcggcc actttccagg ggaatgaaaa ttctggtcaa
     1921 cgcacaggtt gtattccatg agtactacgg ctacagccta accgtactcg acattgaacc
     1981 tgaatacacg ttgggcgata tcgagctgaa acgtcgtgag gttattgcca ggttgatatc
     2041 cgatggcgtg atgaacatga acaaggagct ggagctggac atgcttcccc aacgggttgc
     2101 ggtgatctca tcggcaaatg ctgcggggtt tcaggatttt gtaaaacaac t
//
LOCUS       DAOOQX010000347         2147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_63545_length_2147_cov_3.514818, whole genome shotgun sequence.
ACCESSION   DAOOQX010000347 DAOOQX010000000
VERSION     DAOOQX010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63545_length_2147_cov_3.514818"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="PLX53_06195"
     CDS             complement(<1..1379)
                     /locus_tag="PLX53_06195"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOY72709.1"
                     /translation="MNADSSDSQVMERAKQWLSPVFDMETREQVQQLISGNHDELVES
                     FYRDLEFGTGGLRGIMGVGTNRMNIYTVGIATQGLANYLKKQLPGIPEVRAAIAFDSR
                     NNSIKFAQIAAKVLSANGVRVYLFDSLRPTPELSFAIRTLKCHCGIVVTASHNPKEYN
                     GYKVYWEDGAQVVPPHDSGIIAEVQAIRNISEVNFNGNESLISTIGPEMDEIYSNTLS
                     SLSLSPKIIKKHKEIVIVYTPIHGTGVKLVPKTLRKFGFTNIVNVPEQDVVDGNFPTV
                     SSPNPEEPSAMRLALEKAKEVNADVVMATDPDADRVGLGVRTPEGKYTLLNGNQAASI
                     LIYYVLQKWHEANRIKGREFIVKTIVTTDLLAEIARKYGVECYEVLTGFKYIAEKIRQ
                     LEGEKKFIAGGEESYGYLCGDFVRDKDAVISCALFAEATAWAKEQGKSLYEVLAGIYR
                     EFGLYSEKQL"
     gene            complement(1397..>2147)
                     /gene="rfbD"
                     /locus_tag="PLX53_06200"
     CDS             complement(1397..>2147)
                     /gene="rfbD"
                     /locus_tag="PLX53_06200"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOY72710.1"
                     /translation="LDITSDQQVDNFFQQNHFDYLVNCAAYTAVDRAEDEPEKAFLIN
                     GHAPGLLAEACKSHGTKLFHISTDYVFDGMSNSPYHESSPVNPLSVYGKSKLMGENKV
                     LESGVGMVIRTSWLYSTTGNNFVKTILRNASSKAQLNVVFDQVGTPTLADDLAAVLLQ
                     IIEKGDSSFRPEIFHYSNEGVCSWYDFAYEIVAIAGLPCRINPIETNDYPTRAIRPRY
                     SVLSKEKIRNLLPIEIPYWRDSLKTFLKEML"
BASE COUNT          545 a          522 c          482 g          598 t
ORIGIN      
        1 agctgctttt cgctgtaaag tccgaattcc ctgtaaatac cagcaagaac ctcatagagc
       61 gatttcccct gttccttagc ccaggcagtg gcctcagcaa acaaggcgca gctgataacg
      121 gcatccttat cgcgaacaaa atcaccgcac aggtagccgt agctctcctc gccaccggca
      181 ataaactttt tttcaccctc cagctgcctt atcttttcag caatgtactt gaaaccggta
      241 agcacctcgt agcactccac tccgtatttt cgggcaattt cggctagcaa atcggttgta
      301 acaatcgttt ttactataaa ctccctgccc tttattcggt tagcttcgtg ccatttttga
      361 agtacgtagt aaataagaat tgatgcagcc tgattgccgt tgagaagggt gtacttgcct
      421 tcaggagttc taactccaag ccctaccctg tcggcatcgg ggtcggtagc catgaccaca
      481 tcagcattta cctccttggc tttttcaagt gccaacctca tggccgatgg ttcctcgggg
      541 ttgggtgatg aaacagtggg aaagttgccg tcaaccacat cctgctcggg aacattcact
      601 atgttggtga aaccaaactt acgaagggtt ttgggaacca gcttcacccc ggtgccatgt
      661 attggggtat aaacaatgac gatttcctta tgttttttaa tgattttggg agagagcgaa
      721 agcgacgaaa gggtattgga gtaaatctca tccatctccg gtcctatagt gctaatcagg
      781 ctttcgttgc cgttgaaatt cacctccgaa atgttcctaa ttgcctgaac ttctgcaatg
      841 attcctgagt cgtgaggtgg aacaacctga gcgccatcct cccaataaac cttgtatccg
      901 ttgtattcct tggggttatg cgaagcggtt acaacaatgc cacaatggca tttaagcgtt
      961 cgaatagcaa aactcagctc gggagtaggc ctgagggaat cgaaaaggta aacccgcacc
     1021 ccatttgcgc taaggacttt ggcagcaatt tgggcaaatt ttatgctgtt gtttcgggaa
     1081 tcaaaggcaa tggcagccct aacctcagga ataccaggaa gttgcttttt cagatagttt
     1141 gcaagccctt gcgtggcaat gcccacggtg tatatattca ttcggtttgt accaacaccc
     1201 ataatccccc ttagtcctcc cgtaccaaat tccaaatctc tgtaaaacga ctcaaccagc
     1261 tcgtcgtggt taccactaat aagctgctgc acttgctccc tcgtttccat atcaaaaaca
     1321 gggcttagcc attgctttgc cctttccatc acttggctat ccgatgagtc agcattcatg
     1381 gcatttcatt ttagggttat agcatttctt ttaggaaagt tttaaggcta tccctccagt
     1441 agggaatttc gattggcagc aagttacgaa ttttttcttt actcaacacg ctatacctgg
     1501 gtcgaatggc acgagttggg taatcatttg tttcgatggg atttatacga caaggtaggc
     1561 cggcaattgc cacaatttca taggcgaagt cataccagct gcaaacacct tcattcgagt
     1621 aatgaaatat ttcgggacga aaggagctat cgcccttttc tataatttgg agcaatactg
     1681 ctgccaaatc atcggcaaga gttggggtgc caacctggtc gaaaaccacg ttgagctgtg
     1741 cctttgatga ggcattcctg agtatggttt taacaaagtt gttccccgta gtggagtata
     1801 accacgaagt gcgaattacc atgccaactc ccgattctaa cactttgttt tcgcccatga
     1861 gcttgctttt gccataaacc gaaagcggat tgaccgggga agattcatgg tagggcgagt
     1921 tggacattcc atcgaaaacg tagtcggtgg atatgtgaaa gagtttggtt ccatggctct
     1981 tgcatgcctc ggccagcaac ccgggcgcat gaccgttaat cagaaatgcc ttttcaggct
     2041 catcctcggc acgatcaacc gcagtgtagg cagcacaatt tacaaggtaa tcaaaatggt
     2101 tttgctgaaa gaaattgtca acctgttggt cgctggttat atccagc
//
LOCUS       DAOOQX010000348         2136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_64028_length_2136_cov_2.213359, whole genome shotgun sequence.
ACCESSION   DAOOQX010000348 DAOOQX010000000
VERSION     DAOOQX010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64028_length_2136_cov_2.213359"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1978)
                     /locus_tag="PLX53_06205"
     CDS             complement(<1..1978)
                     /locus_tag="PLX53_06205"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOY72711.1"
                     /translation="MKKFADYTLMALLAGMLACSNNNEQLVTSPSGEVKVIFSLTADK
                     QPSYRVYFKDSPVIETSTLGFDFKDAPSLSKGMRVDSVLSNEFNETWELPWGEQRLVK
                     NHYNEMKVFLSEKKSPNRHFAVTYRVYDDGIGFRYEFPQQESMGDVTITDENTQFKLT
                     GDHLCWWEPGDWDIYEHLYNTTSFSKIDATKKRNHRDLAQTYIPENAVNTPVTMKTSQ
                     GLYLSFHEAAVNNYAGVTLKVDTVNYSMTTELVGNDSGVKVVTKTPFNTPWRMIQLAE
                     KPGDLIESRLILNLNEPNKLGDVSWFKPTKYVGIWWEMHLGKSSWDMASGKHGATTAN
                     AKRYIDFTAKNNIGALLVEGWNTGWEHWIGFDDREGVFDFVTPYPDYNLKEVVEYGRK
                     KGVDLIMHHETSSAVTTYDKQLDTAFALISSLNLHMVKTGYVGKIIPKGEYHHGQWMV
                     NHYRRVLEVAAKHKVAVDVHEPFMATGERRTYPNFVSQEGFRGQEFNAWSSDGGNPPE
                     HLAIIPFTCLLAGPMDYTPGIFNTKLKPYKPNNQVNNTLAQQLALYVVIYSPIQMAAD
                     LPENYENHPAFQFIRDVGVNWEQTRVLNGEVGDYVTIARQERGTNRWFIGAVTDENSR
                     ELSVKMDFLEPGKKYSATLYLDAPDAHWDKNASAY"
BASE COUNT          535 a          519 c          460 g          622 t
ORIGIN      
        1 cgtatgccga ggcgttctta tcccaatggg catcaggagc atcaaggtaa agggttgctg
       61 aatacttttt acccggttcc agaaaatcca ttttaacgct gagctccctt gaattctcat
      121 cggttaccgc accaataaac caacggtttg ttccgcgttc ctgacgggca atggtaacat
      181 aatcgccaac ttcaccgtta agcaccctgg tttgctccca gtttacaccc acatcacgga
      241 taaactggaa tgcaggatgg ttttcataat tctcaggcaa atcggcagcc atttgaattg
      301 ggctgtagat gactacataa agggcaagct gctgtgccag tgtattattt acctggttgt
      361 tgggtttgta gggtttcagc ttggtgttga agattcccgg tgtatagtcc ataggtccgg
      421 ccagcaggca ggtgaaggga ataatagcta aatgctcagg aggatttccc ccatcgctcg
      481 accatgcgtt gaactcctgt ccccggaatc cctcctgaga aacgaagttt ggatatgttc
      541 tgcgctcgcc tgttgccata aacggttcat gaacatcaac ggcaaccttg tgctttgcgg
      601 ccacttcaag aacccttcgg tagtggttaa ccatccactg cccatgatgg tactctcctt
      661 tgggtattat cttccctacg tatcctgttt taaccatgtg gaggttcagg ctgctgatta
      721 gggcaaaggc ggtatcgagc tgcttatcgt aggtggtaac cgccgatgat gtttcgtggt
      781 gcataatcag atcaacaccc ttcttgcggc catactcaac cacctccttg aggttgtagt
      841 ccggataggg ggttacgaag tcaaaaactc cctccctatc atcaaatcca atccagtgct
      901 cccaaccggt attccaaccc tctacaagta aggctccgat attattctta gcggtaaagt
      961 ctatatatcg tttagcattt gcagtagttg ccccatgctt cccactggcc atatcccagc
     1021 ttgattttcc caggtgcatc tcccaccata taccaacgta cttcgtgggc ttaaaccatg
     1081 aaacatcgcc cagcttgttg ggctcgttca ggttaagtat cagccttgat tctatcaaat
     1141 caccaggttt ttcggcaagc tgaatcatac gccagggagt gttaaagggt gttttggtaa
     1201 ccaccttaac tccagaatcg ttgccaacca gctcagttgt catggagtag ttaaccgtat
     1261 caacttttaa ggttactccg gcatagttat taacagcggc ttcgtgaaag ctaaggtata
     1321 gcccctggct agttttcatg gttacaggag tatttacggc gttttccgga atgtaggttt
     1381 gtgccaaatc gcgatggttt cgctttttgg tggcgtcaat cttgctaaac gaagttgtgt
     1441 tatatagatg ctcatatata tcccagtcgc cgggctccca ccagcacaaa tggtcgccgg
     1501 ttagcttaaa ctgtgtattt tcatcggtga ttgttacatc gcccatactt tcctgttggg
     1561 gaaattcgta acgaaatcct attccatcgt cgtaaaccct atatgtaacc gcaaagtggc
     1621 ggtttggtga ttttttctcc gacaggaaaa ctttcatctc gttgtaatga ttcttcacaa
     1681 gccgttgctc gccccacggc agctcccacg tttcgttaaa ctcattactc aacacgctat
     1741 caactctcat ccccttggac agcgatgggg catccttgaa atcgaatcca agtgttgagg
     1801 tctcaatcac aggagagtct ttaaagtaaa ccctgtaaga gggctgttta tcggcagtga
     1861 ggctgaaaat taccttcact tctcccgatg gggaagtaac cagttgctcg ttgttgttgc
     1921 tacatgccag cataccggcc agcaatgcca ttagggtata atctgcaaac ttcttcatag
     1981 taaattttac ttttatcttg cccaacaaat atgtataagt ttgaaagtaa acgtgaatac
     2041 ctgtttagaa aatagtatcc cgctacaaca tttattcagg tgaaccaaac tcctgaaatt
     2101 aaaacatcaa tctaaactac cgtatttcag taatat
//
LOCUS       DAOOQX010000349         2132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_64179_length_2132_cov_3.719788, whole genome shotgun sequence.
ACCESSION   DAOOQX010000349 DAOOQX010000000
VERSION     DAOOQX010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64179_length_2132_cov_3.719788"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..>2132
                     /gene="pbpC"
                     /locus_tag="PLX53_06210"
     CDS             106..>2132
                     /gene="pbpC"
                     /locus_tag="PLX53_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOY72712.1"
                     /translation="MNFRRNPTGVWQVLTGITCLLVFLLLPPVRFKSETSTVLLDRNG
                     QLLSATVSDEGMWQFPASDTVPNKFATCITQFEDAYFRFHPGVNPFSMVRAAFQNIKA
                     RKVVSGGSTITMQLVRLSRRGKPRTLGEKIVEMAMAIRTEISYSKPRILAMYSSNAPF
                     GGNVVGLDAAAWRYFGVSPQNLSWAEAATLAVLPNAPGLIFPGRNQHLLVEKRNKLLG
                     KLLKRGYIDDETYHLALLEPSPSLTGGLPHEATHLLTRCIHNGFKGKLVRSTIDRELQ
                     ENIMRVAQNHHGYLLANGIRNMAAMVVDNQTGEVMAYMGNVQSNNLSNSPMVDVISSK
                     RSYGSLLKPFLYGLMLSEGLLYPRQLVNDMPVAYAGFAPQNFSHTFEGVVPANEIISR
                     SLNVPSVNLLQQYGIEKFHSSLKQMGITSINKPAGHYGLSIILGGAEASLWELSGIYA
                     ALARQAQGIAAGNISLRYVLDSTTSSAQPFSCSGIGSGAAYLTLEALTQASRPGEDGT
                     WRNFVSSQKIAWKTGTSYGFRDAWAIGVTTCYTVAVWVGNADGEGRANLIGVATAAPV
                     MFDIFSLLPSSVWFSPPWFDLKPVVTCRQSGHQASPACTDTVTEYVPAVRTKTLPCPY
                     HQKVLVDKTHRYRLTQRCASQEEMVEVSWFVLPPAQEWYYSKLHADYRILPP"
BASE COUNT          541 a          533 c          536 g          522 t
ORIGIN      
        1 attccggcag gtactacatg ccgggtccac actgcgaggc aatgtatgat aacgccattt
       61 ctgccggtga aaaaggaggg tgggtaaccg tggagtagca agccaatgaa ttttagaagg
      121 aatcctacgg gtgtttggca ggtgcttact ggtataacgt gcctgctggt tttcttgtta
      181 cttccccctg taaggttcaa gagcgaaacc tcaactgtgc tactcgaccg taacgggcag
      241 ctgctttcgg ctactgtttc cgatgaagga atgtggcagt ttcctgcctc cgatacggtt
      301 cccaataagt ttgcaacttg cattacccag tttgaggatg cctacttccg gtttcaccct
      361 ggcgtaaacc ctttctcaat ggttcgtgca gccttccaaa atataaaagc ccgaaaagta
      421 gtaagcggtg gcagcaccat aaccatgcag ctggttcgat tgtcgcgacg aggaaaacca
      481 agaaccttag gtgaaaagat tgttgaaatg gcgatggcta tccgaacaga aatttcgtat
      541 tcaaaacctc gaatactcgc catgtattca tccaacgcac cctttggagg aaatgtggtt
      601 gggctcgatg cagcagcttg gcggtacttt ggagtaagcc cgcaaaatct aagttgggcc
      661 gaagctgcta cgctggcagt gctacccaat gcccccgggt tgattttccc cgggcgaaac
      721 cagcacttgc ttgttgaaaa aaggaataag ctgctgggta aactcctgaa aaggggctac
      781 attgacgatg agacctacca tcttgccctg ctggaacctt ccccaagctt aaccgggggg
      841 ctgccccatg aagcaaccca cttgcttacc cgttgcatcc acaacgggtt taagggtaaa
      901 ctcgtacggt caaccattga tagagaactc caggaaaaca tcatgagggt tgcgcaaaac
      961 catcatggat acctcctggc aaatggaatt cgaaatatgg ctgccatggt agtggacaac
     1021 caaacgggtg aggttatggc ctacatgggc aatgttcaat ccaacaatct atccaactca
     1081 ccgatggttg atgtgattag ttcaaaacga agctacggaa gcctcctcaa gccttttctc
     1141 tatgggctaa tgcttagtga gggattactc tacccccgcc agctggttaa cgatatgccc
     1201 gtagcctatg caggatttgc gcctcaaaat tttagccaca cctttgaagg cgttgtgcca
     1261 gccaacgaaa tcatttcccg atcactaaac gtgccttctg ttaacctttt gcagcagtat
     1321 ggaattgaaa aatttcacag cagccttaaa cagatgggga taaccagcat caacaagcct
     1381 gcaggtcact atggtctttc catcatactt gggggcgctg aggctagcct ttgggagcta
     1441 tcgggcatat atgctgctct tgcccggcag gcacagggga ttgctgcagg aaatattagc
     1501 ttacgctatg ttctggatag cacaacatcc tccgcacagc ccttcagctg cagcggaatt
     1561 ggttccggtg ctgcctacct tacacttgaa gcactaacgc aggctagccg ccccggtgag
     1621 gatggaacct ggcgaaactt tgtttcgtcg caaaagattg catggaaaac cggaaccagc
     1681 tatgggtttc gtgatgcttg ggctattgga gtaaccactt gctacacggt agcagtgtgg
     1741 gtgggaaatg ccgatggcga ggggagagca aatcttattg gcgtagccac agctgcccct
     1801 gtaatgtttg acattttttc gctgctccct tcatcggtgt ggttttctcc gccatggttc
     1861 gacttaaaac ctgtagtaac ctgcaggcaa agcggacacc aggcctcacc agcctgtacc
     1921 gatacagtta ccgagtatgt tccagctgtt aggacaaaaa cgctgccttg cccctaccat
     1981 caaaaggtgc tggttgataa gactcatagg tataggctaa cccaacgctg cgcaagccag
     2041 gaggagatgg ttgaggttag ctggtttgtt ctgccgccag cccaggagtg gtactactcc
     2101 aaactacatg cagactatag gattttacca cc
//
LOCUS       DAOOQX010000350         2132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_64190_length_2132_cov_3.058739, whole genome shotgun sequence.
ACCESSION   DAOOQX010000350 DAOOQX010000000
VERSION     DAOOQX010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64190_length_2132_cov_3.058739"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PLX53_06215"
     CDS             complement(<1..892)
                     /locus_tag="PLX53_06215"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOY72713.1"
                     /translation="MGWFGKGLRLKVAFSAMGLLAVITGADAQDAPIRDRLFFGVEPR
                     WGFVMPHHDYMAYFLESNVNSFQLNVGLNTTGYKDWHKSFNYPQLGGGFFYSGLGNDR
                     IYGKMLAAYVFIDRQFLPRKNRFNIGNRAAFGMSYLTKQFDLHSNPYNMVIGTPFNVF
                     FQYDLTLYYRLNSNLNLRWALGFSHASNGNLREPNKGFNLITSGFGLQYSISDDRLLV
                     TQAPGYHSDSTRTYVSIGVLGSAKSVSRENTNLYPVYGMSVEWLRRLSKTSLAGVELA
                     AYKDKAIKYTYQAKDDSTYRS"
     gene            complement(904..1518)
                     /locus_tag="PLX53_06220"
     CDS             complement(904..1518)
                     /locus_tag="PLX53_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOY72714.1"
                     /translation="MKKPGKLCVILIISACPAVVFSQGFKAGFTLGIAATQVDGDTYG
                     GFDKAGPVAGVWVGRNLGNSWLGMMELRYAQKGSYAKTTVNEFNGYYRMRLHYFELPV
                     MGGYRFVNGFQTLGGISLGYLAKAEEMTELGPFPEEDISAFNQFELAMLCGVEYNYSE
                     HWAFGAFFSYSIFPIRPYKGNITYRMNRGQYNQVVELLVRYKIQ"
BASE COUNT          568 a          566 c          455 g          543 t
ORIGIN      
        1 atgacctata tgtgctatca tccttagctt gataggtgta cttaattgct ttatccttat
       61 aggcagcgag ctcaactcca gccagagagg ttttgctcaa acgtcttagc cattcaacgg
      121 acatgccgta aacagggtag aggttggtat tttcacgaga aacagatttg gcacttccca
      181 gaactccaat gctaacgtat gttcgggttg aatcggaatg gtagccgggg gcctgtgtta
      241 ctagtaacct atcgtctgat atggagtact gcagtccaaa accgctggta atcaggttaa
      301 aacccttgtt gggttccctc aggttgccat tggaggcatg gctgaagccc agtgcccaac
      361 gtagattcag gttggagttt aggcggtagt aaagggttaa atcgtactga aagaaaacgt
      421 tgaaaggagt tccaataacc atgttgtagg ggttggaatg caaatcaaac tgcttggtga
      481 ggtagctcat cccaaatgca gcccggttgc caatattaaa cctatttttc cggggcaaaa
      541 actgtcgatc gataaaaacg taggctgcaa gcattttacc atagatacga tcgttgccaa
      601 gcccggagta gaagaacccg ccacccagct gcgggtagtt gaaactctta tgccagtcct
      661 tatatcccgt agtgttaagc ccaacattca gctgaaagct gttcacattg ctctcaagaa
      721 agtaggccat gtagtcgtga tggggcatca cgaatcccca tcgcggttca actccgaaaa
      781 aaagcctatc cctaatagga gcatcctgcg catctgctcc agtgataaca gccagcaatc
      841 ccatcgccga aaatgcgact tttagcctta aacctttacc aaaccaaccc atagcacaat
      901 gatttactgt attttatatc ttaccaataa ttctacgacc tggttgtact gccccctgtt
      961 catcctgtag gtaatatttc ccttataggg acgaatggga aagatagagt agctgaagaa
     1021 tgccccgaaa gcccaatgtt cgctatagtt atactccaca ccgcacagca tggccagttc
     1081 aaactgatta aatgccgaga tatcctcctc gggaaagggg ccgagctcag tcatttcctc
     1141 cgcctttgct aggtatccca ggctaattcc accaagcgtc tgaaagccat tcacaaagcg
     1201 ataccccccc ataacaggta gttcaaagta gtgaagcctc atccggtagt aaccattgaa
     1261 ctcattaact gttgttttgg catagcttcc cttttgagca tagcgtaatt ccatcatgcc
     1321 caaccagctg ttaccaaggt ttcggcccac ccacactccg gccaccgggc cagccttatc
     1381 aaaaccgccg taggtgtcgc cgtcaacctg ggttgcagct ataccaagag tgaaacctgc
     1441 cttaaaccct tgtgaaaaca ccacagctgg gcaagccgaa ataatcagta taacgcaaag
     1501 ttttcctggt tttttcaatt cttaatacct ttttattaat accaccaatg cacgactacc
     1561 catagcagct ggaaaaaact gatttccaac aggaggcata ataagaccca ggctttccgc
     1621 caatgcaact cctcctgaat gaaatacata aaaatttgaa aaggaaaaca aaagtagtaa
     1681 atcttccgct tttagacgat agctaagaaa ccaaatgttt tcgtatgagg ggattactcc
     1741 cccgccctct cacaccaccc ggcttccgcc tgcggcgggc acggcggttc cttttaacgt
     1801 gtaacctctc ataaattgtg catcaaggct cgtcgctgta cgcctgctaa accctcctgc
     1861 tgaaccttca ctccccccag ctccgctttc ggcttcactg gtgtacagcg ctcccggtgc
     1921 tgtcggaaag caactccagc tgatacgcta tagcttgctt ccgctccatt gggtttactt
     1981 gtgtaccggc tgctccccac attaaaagat tcagccgttc atctaccctg gcttgagtaa
     2041 actactatcg cctcagctga ctttttgcgg ggtaaatgct gcatcgctgc cgctcgtgct
     2101 ctcagtaggt gcgccctgca agacctctcg gg
//
LOCUS       DAOOQX010000351         2123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_64583_length_2123_cov_3.616054, whole genome shotgun sequence.
ACCESSION   DAOOQX010000351 DAOOQX010000000
VERSION     DAOOQX010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64583_length_2123_cov_3.616054"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PLX53_06225"
     CDS             complement(<1..631)
                     /locus_tag="PLX53_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72715.1"
                     /translation="MKGLLKYLFALTFAILLSHSIFAQNATRVLYMDTTGKMLYASPN
                     VEVNLYINTTNSASGAVKLTGLEGKGNPLRWSGHGLQVMTHLNLYIGRKIRFELFADG
                     KPPVSAVTTTNQNQKRIDEAVYIPGGAVIEVNAADEDSGLEDTYYSLNGEPFVPYTSP
                     LALDKDGVYEFTVYSTDKLGNREDGVTRKFVVDATPPTSAITVEGDQFEQ"
     gene            complement(633..1589)
                     /locus_tag="PLX53_06230"
     CDS             complement(633..1589)
                     /locus_tag="PLX53_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY72716.1"
                     /translation="MRSWLLTLVLLTYLFGTLAGQERQENLYVNEANQIFVKADAPIY
                     FFVSPSGQPQNMIMLPSSDSLANPMYFDGDGKHYLVYQSSGQKVRYLVYADGVGPNPY
                     VEVARGLLMKHQNRIYLEPNATFTPKAKDKRSGVKSVYYSLNGEPYKLATNELSPGSN
                     GEFSLRIYAVDNVGNTSDTAVYTLISSPDVTFRVNNIYFETASVNLLPESFEVLNQME
                     DMLKLYPELHVEIKAHADTRGNTDYNLDLSERRAQSVVRYLSGKGISKSRLKAVGFGD
                     SQPVNECLKGVQCPDSKHRENRRIEFRFFIPKLMQPTPKLSL"
     gene            complement(1729..>2123)
                     /locus_tag="PLX53_06235"
     CDS             complement(1729..>2123)
                     /locus_tag="PLX53_06235"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOY72717.1"
                     /translation="GQTVEFAGAKNPLFYVTNGQVNQVRGDPVPVGGLQKEKERQYTL
                     HTIHIDTPTCFYIFSDGYIDQFGGPYNQKFSSQQFKELLLKVHQLPMIQQQEILETTM
                     DEWQGSENKQIDDIMVIGFRLNAKPLGI"
BASE COUNT          512 a          469 c          535 g          607 t
ORIGIN      
        1 cttgttcaaa ctgatcacct tcaacggtaa tagccgatgt aggaggtgta gcatcaacca
       61 cgaatttacg ggttacccca tcctcccggt tgcccagctt gtcggttgag tacacggtaa
      121 actcgtaaac tccatccttg tcgagtgcca ggggagaggt gtagggaaca aagggttcgc
      181 cgttgagaga gtagtaggta tcctcaaggc ctgaatcctc atcggcggca ttcacctcaa
      241 ttaccgctcc gccaggtatg tacacagcct cgtcaatacg cttctggttc tggttggtgg
      301 tggtcaccgc tgatacaggc ggtttcccat cggcaaacag ctcaaaacga atttttcggc
      361 caatgtagag gttaagatga gtcatcacct gaagtccgtg tccgctccag cgcaaaggat
      421 tcccttttcc ttccagacct gtgagtttta ctgcacctga ggctgagttg gttgtgttga
      481 tgtagagatt tacctccacg ttgggtgatg cgtagagcat ttttccggtt gtatccatgt
      541 acaggacacg tgtggcattc tgggcaaaaa tgctatgcga taaaagaatg gcaaaggtta
      601 gggcgaatag gtattttagc agtcctttca ttttataagc tgagtttggg tgtgggctgc
      661 atgagttttg gaataaagaa gcgaaattca attctccggt tctcccggtg cttgctatca
      721 ggacactgca cccctttcag gcattcgttt accggttgcg aatcgccaaa cccaacggcc
      781 ttcagcctgc tcttgctgat gcccttgccc gaaaggtaac gaacaaccga ttgtgccctt
      841 cgttcggaca agtcaaggtt ataatcagtg tttcctcgcg tgtcggcatg tgcttttatt
      901 tcaacatgga gttcggggta gagcttcagc atgtcttcca tttgatttaa aacctcgaat
      961 gattcgggta gcaggtttac gctggcggtt tcaaagtaaa tgttgtttac cctgaatgta
     1021 acatcgggag aagaaattag ggtgtatact gcggtatcgc ttgtattacc aacattatca
     1081 acagcataaa tacgaaggct gaattctccg ttgctacccg gcgacaactc gttggtggcc
     1141 agcttgtagg gttcgccgtt taaggagtag taaacagatt ttacccccga acgtttatcc
     1201 tttgccttag gagtgaaggt tgcgttgggt tcaaggtata tgcggttctg gtgcttcata
     1261 agcaggcccc gggcaacctc gacgtagggg ttgggaccaa ctccatcagc atacaccagg
     1321 tagcgaactt tttggccgct actctggtaa accaggtagt gctttccgtc accatcgaag
     1381 tacattgggt tagccaggct atcgctgctt ggcagcataa tcatgttttg gggttgacca
     1441 gaaggagaaa cgaaaaagta aatgggggca tcggctttaa cgaatatttg attcgcttcg
     1501 ttgacgtaaa gattttcctg ccgctcctgg ccggctagag ttccaaacaa atatgtcagc
     1561 agaacaaggg taagcaacca acttctcata ttgcgctctt tgtcgggaca aatttataat
     1621 attttcttgc tcaattccct aatcgtaaaa attacttttt ttagatcaac aagggagcag
     1681 atgaaggcat cgggtttgtg aaatggccga cagcattgct tttgggtgct atatgcctaa
     1741 aggtttggcg ttaagcctaa aaccaatgac cattatatcg tcgatctgct tgttttcact
     1801 tccctgccat tcatccatag tagtttcaag aatttcctgt tgctggatca tgggaagctg
     1861 gtgaacctta agcagcagct ccttgaattg ctgtgatgag aatttctggt tatacgggcc
     1921 tccaaactgg tcgatgtagc cgtcggagaa aatgtagaag caggttgggg tgtcaatatg
     1981 gatggtgtgg agggtatatt ggcgttcctt ttctttttgc agtccaccaa caggcacagg
     2041 atcgcctcta acctggttaa cctgaccgtt ggttacatag aatagagggt ttttagcgcc
     2101 ggcaaattca actgtttgtc cat
//
LOCUS       DAOOQX010000352         2123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_64595_length_2123_cov_2.901354, whole genome shotgun sequence.
ACCESSION   DAOOQX010000352 DAOOQX010000000
VERSION     DAOOQX010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64595_length_2123_cov_2.901354"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..396
                     /locus_tag="PLX53_06240"
     CDS             160..396
                     /locus_tag="PLX53_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOY72718.1"
                     /translation="MAKIRGAIVVDVERCKGCSLCVVACPSSVIALAKEVNGKGYNYA
                     YMVNPDACTGCVNCAVVCPDTVISVYKVKADAVA"
     gene            438..1523
                     /locus_tag="PLX53_06245"
     CDS             438..1523
                     /locus_tag="PLX53_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOY72719.1"
                     /translation="MAELKLMKGNEAIAEAAIRCGCDGYFGYPITPQSEIMETLMARQ
                     PWDETGMVVLQAESEVASINMVYGGAACGKRAMTSSSSPGISLMQEGITYIAGSELPC
                     LIVNVVRGGPGLGTIQPAQSDYFQAVKGGGHGDYKLIVLAPASVQEMYDFVDLAFELA
                     FKYRNPAMILSDGVIGQMMEKVQIGDYKPRLTDEEVVKRYGSWATTGKPSTRERNIIT
                     SLDLDANSQELFNLKLLKKYAEIEKNEIRFEEISCTDAEYLLVAYGSSSRICQKTVDL
                     AREKGIKVGLLRPITLYPYPYGAIQKLVPQLKGILAVEMSAGQMVEDVRLAVNGKVPV
                     LHYGRMGGVIPTPNEVLKVLEDKLIKG"
     gene            1528..>2123
                     /locus_tag="PLX53_06250"
     CDS             1528..>2123
                     /locus_tag="PLX53_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOY72720.1"
                     /translation="MDVKDIIKEENLVYSKSKVLTDTIMHYCPGCTHGVVHKVIAEVI
                     EELGIQDKTIGVSPVGCAVFAYNYLDIDWQEAAHGRAPALATAINRLYPDKYVFTYQG
                     DGDLASIGTAEIIHACNRGENIVVIFINNGIYGMTGGQMAPTTLIDMPTSTTPYGRKV
                     DLNGYPLKITDLVAQLPGTCFVTRHSAHTPAAVRKLKKA"
BASE COUNT          607 a          474 c          521 g          521 t
ORIGIN      
        1 cgaacgttcg gaaggcttgc gtgcatgtgt ttcacaagtg cagagaagcc agttttcgga
       61 cttagagtac cgccttaaaa aatttcacga agatcatcca accaagtaat attatagcaa
      121 gctaactatt atacaaacac ttttaaattt ttcaatgtca tggcaaaaat aagaggagca
      181 attgttgttg atgtggaaag gtgcaaggga tgcagccttt gtgttgttgc ctgcccttcg
      241 agcgtcattg cgctggcaaa ggaggtaaac ggcaagggtt acaactatgc ctacatggtc
      301 aaccccgatg catgcacagg atgtgttaac tgtgccgttg tatgtcccga cactgttatt
      361 tcagtttaca aagttaaggc tgatgcagta gcctagagta gcattactta aagttgaaaa
      421 tacaaatttc aaaagaaatg gcagaactaa aactaatgaa ggggaacgaa gctatagccg
      481 aggcagctat ccgatgtggt tgcgacgggt attttggcta ccccatcacc cctcaatcag
      541 aaatcatgga aacccttatg gccagacagc cgtgggatga aacaggaatg gtggtgcttc
      601 aggcagagag cgaggtggca tccataaaca tggtatatgg aggtgccgct tgtggaaaaa
      661 gagccatgac ctcgtcgtca agccccggta taagcctaat gcaggaaggt ataacctaca
      721 ttgccggatc tgagctgccc tgtctaatag ttaatgtagt tcgtggaggg cctggccttg
      781 gtaccattca acctgcccaa agcgactact ttcaggctgt taagggaggc ggacatggcg
      841 actacaagct tattgttctg gctccggcct cagtccagga aatgtacgat tttgtagatt
      901 tagcatttga gctggccttt aaataccgta atccggccat gattctctcc gatggcgtta
      961 ttggccaaat gatggagaaa gttcaaatag gcgattacaa gccgcggctt actgacgagg
     1021 aagttgtaaa acgttatggt tcgtgggcaa ccaccggtaa gccttcaacc cgtgagcgca
     1081 acattatcac ctcccttgac cttgatgcca acagccagga gctgttcaac cttaagctgc
     1141 ttaagaaata tgcggaaata gaaaaaaatg aaatccgttt cgaggaaatt tcctgcaccg
     1201 acgcagaata cttgctggtt gcttacggct catcgagcag aatatgccaa aaaaccgttg
     1261 atttggcccg tgaaaaggga atcaaggtag gattgttacg acccattacc ctttaccctt
     1321 acccctatgg agccattcaa aagcttgtgc cacaactaaa gggaatttta gctgtcgaaa
     1381 tgagcgccgg tcagatggtg gaggatgttc gactagcggt taacgggaaa gtccctgttc
     1441 tccactacgg gagaatgggt ggtgtaatac ccaccccaaa tgaggtgtta aaggttctgg
     1501 aagataaact tattaaaggc taaaacgatg gacgttaagg acataatcaa agaggaaaat
     1561 ttggtttaca gcaaatcaaa agttctcacc gatacaatta tgcactactg cccgggctgc
     1621 acccatggtg ttgtgcacaa ggtaatcgcc gaggtaattg aagagctggg cattcaggat
     1681 aaaaccatag gggtttcacc agtgggctgt gccgtatttg cctataacta cctcgatatc
     1741 gactggcagg aagctgctca tggtagagct cctgctctgg ctaccgccat taaccgactc
     1801 taccccgata agtatgtgtt tacctaccag ggtgatggcg atttagcttc catcgggact
     1861 gcagagataa tccatgcatg caaccgtggt gaaaacatag tagtaatatt catcaacaac
     1921 ggaatatacg gtatgaccgg tggtcaaatg gcgcctacta cgctgataga catgccgacc
     1981 tccaccacgc cctacgggcg taaagttgac cttaatggtt acccgttaaa aattaccgac
     2041 cttgtggcac agctgcccgg tacatgcttt gttaccagac attcggccca cactcctgct
     2101 gccgttagaa agcttaagaa agc
//
LOCUS       DAOOQX010000353         2120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_64726_length_2120_cov_2.917191, whole genome shotgun sequence.
ACCESSION   DAOOQX010000353 DAOOQX010000000
VERSION     DAOOQX010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64726_length_2120_cov_2.917191"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1198
                     /gene="galE"
                     /locus_tag="PLX53_06255"
     CDS             176..1198
                     /gene="galE"
                     /locus_tag="PLX53_06255"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOY72721.1"
                     /translation="MDKILVTGGAGYIGSHTVVELVGAGYEVVLVDNFSNSSPEVLQG
                     IETITGVKPTFVEADCSDISAMRQLFSTHPGIQGIIHFAALKAVGESVDKPLQYYHNN
                     LLSTINMLAMMNEFKVKNLVFSSSCTVYGQPDELPVTEQSPMKPALSPYGNTKKICED
                     IIRDAAAANPDIRAIALRYFNPIGAHPSVYIGEQPRGKPDNLVPFITQTAAGVRDMLQ
                     IFGGDYHSPDGTAIRDYFHVVDLAKAHILAVERLMWGKNKSNFEVFNVGSGRGVTVLE
                     IVRAFMAVTGVNVPYEVVGHRAGDIEKVWADTSLAQKELGWKPESSLEDALLSAWRWE
                     KKLRGL"
     gene            1562..>2120
                     /locus_tag="PLX53_06260"
     CDS             1562..>2120
                     /locus_tag="PLX53_06260"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOY72722.1"
                     /translation="MKHAVLFLLGFPLPALAQPDSTSMKVNLDTVWVFSPQRVSSYSE
                     LSRMVGVVKRSELRQMKATSLSEVLERSASIDIRQRGTHGVQADINFRGGSFDQTLVM
                     LNDINISDPQTGHHNLNIPVVLDAVSRIEILQGPGARELGQGAFSGAINIITGPSAKN
                     EFSLSTFAGEYGLLKTSASATFGSAK"
BASE COUNT          524 a          491 c          502 g          603 t
ORIGIN      
        1 gcccctctta attccccttc ccatccggtt gaatagctca attaggtcaa cctcgtcggt
       61 tgctttagtt ttatttactt gctcgtcgct catatattcg tgatttagtg attctctttt
      121 ctgtaaatcc tgatttggca acaaatatat tacttttgtt caaaagtatc taaaaatgga
      181 caagattctt gttacaggtg gagccgggta tattggttcg cacacggttg ttgagcttgt
      241 tggcgccggg tatgaagtgg tgctggttga taatttttcc aactcctcac cggaggtgct
      301 acagggaata gagacgatta ccggcgttaa accaactttt gttgaggccg attgctccga
      361 tatttcggcc atgcggcagc tgttctcaac ccatcccgga attcaaggca ttattcattt
      421 tgctgcactg aaggctgtag gcgagagtgt ggataagcct ctccaatact accataacaa
      481 ccttctttcg accattaata tgctggccat gatgaacgaa ttcaaggtga aaaacctggt
      541 gttctccagc tcctgcacgg tgtatggtca acccgacgag ctgcccgtaa ccgagcaatc
      601 gcccatgaaa ccagctcttt cgccctatgg caataccaaa aagatttgcg aggatatcat
      661 tcgcgacgcc gctgcggcaa atcccgatat aagagctatt gcactaaggt actttaaccc
      721 cattggtgcg cacccttctg tctacattgg cgaacaaccc aggggaaaac ccgacaacct
      781 ggtgccattt atcacccaaa ccgctgcagg ggtaagggac atgctccaaa tatttggagg
      841 cgactaccat tcccctgatg gaacagccat tcgcgactac ttccacgttg ttgacctggc
      901 taaagcccac attctggctg ttgaaaggtt gatgtggggg aaaaacaagt ctaactttga
      961 ggttttcaac gtgggtagcg gacgtggtgt tactgttctg gaaatagtta gggcatttat
     1021 ggcggttacc ggggtaaacg ttccctacga ggtagtaggc caccgtgccg gtgatatcga
     1081 aaaggtttgg gccgatacat cccttgctca aaaggagctg ggttggaaac cggaaagtag
     1141 cctcgaggat gccctgctct cggcatggcg ctgggagaaa aagttgagag gattatagca
     1201 ccctactagc ctcttccggc cttttctgag gttcataagt ttttagattg gacgtgcgga
     1261 agcgtccaat tcttttttac caatcccaat acattcttcc atattaacac cagcagcaag
     1321 ttgatggctt tactcatcat gtttattaac atagtcatag ggtgatgttt acctaccggc
     1381 ttgtaatact tttgcagcgt gtttttaatc catactacac cattgatttt ttgtaagggt
     1441 tttctataaa actttgggcg tttgcctacg acacgtcctt tggggttcat ttcagcacaa
     1501 acaagtgtag ggtgttgata tatagaatat tatcaattaa tagcactaaa aatttgtatg
     1561 gatgaagcat gccgtacttt tcctgttggg attcccgttg cctgctttag cacagcccga
     1621 ttcaacttcc atgaaggtaa atttggacac tgtatgggtt ttttccccgc aaagagtttc
     1681 gtcctactcg gagcttagtc ggatggttgg cgttgtcaaa aggagcgagc tgcgccagat
     1741 gaaggcaact tccctttcgg aagtgctgga acgctccgca tctattgata ttcgtcagcg
     1801 gggtactcat ggtgtgcagg ccgatattaa ctttagggga ggatcgtttg accagacgct
     1861 tgtcatgctc aacgatataa acatcagcga tccacaaacc ggtcaccata acctgaatat
     1921 tcccgtcgtt ctcgatgcgg ttagtcgtat tgaaatactt caaggccccg gggcccggga
     1981 gttgggacag ggcgcttttt caggagccat caatataatc acagggcctt cggcaaaaaa
     2041 cgagttctca ctttcaactt ttgccggtga gtacggcttg ctgaaaacct ctgctagcgc
     2101 aacatttggt tcagcaaaaa
//
LOCUS       DAOOQX010000354         2116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_64903_length_2116_cov_4.180495, whole genome shotgun sequence.
ACCESSION   DAOOQX010000354 DAOOQX010000000
VERSION     DAOOQX010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64903_length_2116_cov_4.180495"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PLX53_06265"
     CDS             complement(<1..636)
                     /locus_tag="PLX53_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOY72723.1"
                     /translation="MALLLMAATGFSTGGCKSGNEQAEPFKWQVDQFADLRIMRYQIP
                     GFDSLTPKQRVLVYYLSEAAKCGRDIIWDQNYKFNLDVRSVLEGVYQGYKGNRNDPRW
                     GSFMVYLKRVWFSNGIHHHYSTDKIMPDFDRSYFAELVGNTPSEMLPKKFGGVDSILA
                     HFTPIIFDADVAPKRVNQLAGSDLVTTSACNYYEGVTQAEAEEFYSRLANPN"
     gene            complement(688..1614)
                     /locus_tag="PLX53_06270"
     CDS             complement(688..1614)
                     /locus_tag="PLX53_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72724.1"
                     /translation="MRKVILINVAAALVILPLVGFGQEVNANFFSTREDSLSKLLSLI
                     VSETSTTRRDSLNRIFHENFKNTLEHDGAIDYPFSSLKHVGMLTSSDQKVRIITWNIP
                     QTGGYHRYFGFVLVRLDGDKSRLYALTDSREMVKDPQHERLPADRWLGALYYQLAELP
                     YGSEKQYVLLGFDYNNLFTSKKVIEVLTLSPQGEPVFGLPVFNVDNKQLLSRIVFEFS
                     ARAGFNLKYYDEQKIIAFDHLSPSQPEFTGDFQFYGPDASIDGFAFENGKWVYVRDLD
                     MRNPSREKVNPVDAPEKIDEPPFLYKPQQKRK"
     gene            complement(1618..>2116)
                     /locus_tag="PLX53_06275"
     CDS             complement(1618..>2116)
                     /locus_tag="PLX53_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOY72725.1"
                     /translation="ALERFGVTPDILLLAKALGGGMPLGAFISSQKIMNCLMENPSLG
                     HITTFGGHPVSCAASLAAIDVLTGDRLPESVPEKEQIFRDILSKHPLVKEFRSFGLLM
                     AIELGSFEKVSALIKYGVEHGFVLDWFLFCNTAIRLAPPLCITKEQCEEVANLIYQGL
                     NEINR"
BASE COUNT          511 a          487 c          538 g          580 t
ORIGIN      
        1 gttggggttg gccagcctgg agtagaattc ttctgcttcg gcctgtgtaa caccctcgta
       61 gtagttgcag gctgaggtgg ttaccaaatc agatccggca agctggttaa ctcgttttgg
      121 ggctacgtcc gcatcgaaaa ttatgggtgt aaaatgtgcc agaatgctat cgacgccccc
      181 aaatttcttg gggagcatct cggacggggt gttaccaacc agctcggcaa agtagcttcg
      241 gtcgaaatcg ggcataattt tatcggtgga gtagtggtgg tgtatgccgt tggagaacca
      301 aacccgcttc aggtaaacca taaagctacc ccatcgtggg tcgttgcggt ttcccttgta
      361 accctggtaa actccctcca gaactgagcg cacatcgagg ttgaacttgt agttttggtc
      421 ccaaatgata tccctaccgc atttagctgc ttcactaagg tagtacacca gcaccctttg
      481 tttaggtgtg agcgaatcga agcccggaat ttggtagcgc atgatgcgga ggtcggcaaa
      541 ctggtcaact tgccacttaa aaggttcggc ctgctcgttt cccgatttac atccaccagt
      601 cgagaacccg gtagcggcca ttaacaatag tgccattatt tttctgttca tttttagttg
      661 agttgcaaaa tgtatgaggt gtgtatgcta ttttctcttt tgctggggtt tatatagaaa
      721 tgggggttcg tctatctttt ctggtgcgtc aacaggattt actttttctc tggaggggtt
      781 acgcatgtcc aggtccctaa cataaaccca ttttccattt tcaaaggcaa aaccatctat
      841 tgaggcatca ggcccgtaga actgaaaatc accggtaaat tcgggttggg agggcgacag
      901 gtgatcgaat gcaattattt tttgctcatc atagtacttg aggttgaaac cggctcttgc
      961 gctaaactca aaaacaattc tcgaaagcag ctgcttgtta tccacgttaa aaactggcag
     1021 gccaaaaaca ggttctccct gggggctgag ggtgagcacc tcgattacct tttttgaggt
     1081 gaagaggttg ttgtagtcga atcctagcaa cacgtactgc ttttctgagc cgtagggtag
     1141 ttctgccagc tggtagtaca gagctccaag ccacctgtcg gccggtaacc tttcgtgttg
     1201 cgggtccttt accatttccc ggctatccgt gagggcataa agtcgggatt tatctccatc
     1261 cagtcgaacc agaacaaacc cgaagtagcg atggtagcca cctgtttggg gaatattcca
     1321 ggtgattatc cttacctttt gatcactgga agtgagcatt cccacgtgtt ttagtgagct
     1381 gaatgggtaa tctattgccc catcatgctc aagagtgttt ttaaagttct catggaaaat
     1441 tctattcagc gagtcgcgcc gggtggtgga tgtttcgctt acgatgagtg agagcagctt
     1501 agaaagcgag tcctcgcggg tagaaaagaa gttggcatta acttcttgtc caaaccccac
     1561 aaggggtaga ataaccaggg cagccgcaac gttaattagg attactttgc gcataagcta
     1621 gcggtttatt tcgtttagac cttggtagat taggttggcc acctcttcgc attgctcctt
     1681 tgttatgcaa aggggaggtg caagcctgat ggccgtgttg caaaatagaa accagtccag
     1741 cacgaaacca tgttccactc catattttat taaggcgctg accttctcaa acgaacctag
     1801 ctctatggcc atcagcagac cgaatgaccg aaactcttta accagcgggt gtttcgatag
     1861 gatatccctg aatatttgct ccttttccgg aacgctttcg gggagcctgt cgccggttaa
     1921 aacgtcaatt gcggcgagcg atgcggcaca ggaaaccgga tgacctccaa aggttgtaat
     1981 atgcccaagg gaagggtttt ccatgaggca gttcattatt ttctgtgagg aaatgaatgc
     2041 ccctaggggc ataccaccac ccagcgcctt tgctaggagc aggatgtcgg gtgtaacccc
     2101 aaatcgctct agcgca
//
LOCUS       DAOOQX010000355         2109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_65255_length_2109_cov_4.125122, whole genome shotgun sequence.
ACCESSION   DAOOQX010000355 DAOOQX010000000
VERSION     DAOOQX010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65255_length_2109_cov_4.125122"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(327..1409)
                     /gene="wecB"
                     /locus_tag="PLX53_06280"
     CDS             complement(327..1409)
                     /gene="wecB"
                     /locus_tag="PLX53_06280"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOY72726.1"
                     /translation="MNSKLILTVLGARPQFIKAAAVSRQLMAFGLEEVIVHTGQHFDE
                     NMSEIFFKEMCIPAPKYNLEVHGLNHGAMTGRMMELLENIVINEKPAAVMVYGDTNST
                     LAGALVAKKLNTRVVHVEAGLRSFNMTMPEEINRILTDRISDLLFTPTEAADSNLMAE
                     GFSHFNAKIIRSGDVMQDAAYFYFNRAKTESHVLERLNLPEHFVLATFHRQENTDNLN
                     NLQGIVDGLNRINSETQVVVPLHPRTAKILERVSISPNFRIVEPLGYFDMLMLLNQSS
                     LVVTDSGGLQKEAFFFGKHCVTLREQTEWVELVEKGYNMLSGTSSDSIYNSFRQMIAR
                     KSNFKVNLYGNGHAAEKIAAELKDWL"
     gene            complement(1402..>2109)
                     /locus_tag="PLX53_06285"
     CDS             complement(1402..>2109)
                     /locus_tag="PLX53_06285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY72727.1"
                     /translation="VVREADGVIVVSAQMKNELMVLRPKRINLITNGFDEEDWPNEPV
                     DLDSKFSITHVGTLSRYFNPVILWEVLSDLCEEIEGFRNDLVIRLVGQVDATAIESIQ
                     KVNLQNNLESLGQVTHVEAVKFQRKSQVLLLVVNSSPNAMGILTGKVFEYLASGRMVL
                     AVGPTYGDLASLLSETKAGFFVDYNDKAKTKRVITHLYNLYKENNLRPETKDILRYSR
                     KELTRTLSNLLNEMTNE"
BASE COUNT          543 a          479 c          451 g          636 t
ORIGIN      
        1 gcatactttt ttgtgtcaag acaaaaaagt atgtggggtt tggggcaaag ccccaagatt
       61 tcatgataaa aacttatcca gcttttaaca aactcacaaa ttcattctgc acctttatgg
      121 cttaacactt ctctccggaa cgccaaaagg aactttgccc catgctagct attgcgtccc
      181 gttggggctg aagaaaaagt aaggtaatct tgcccggctt tttaccttta acgcttcaca
      241 atgcacatct gctcttctgg attttatttt cttcctcttt gcttcaaaat gggttgtaag
      301 agtttttatt gaatctgggt tttagcctaa agccaatcct ttagctctgc tgcaattttc
      361 tccgctgcat gcccattccc gtaaaggtta accttaaagt ttgattttcg ggcaatcatt
      421 tggcggaagg agttgtatat gctgtcggag ctggttcctg aaagcatgtt gtagcccttc
      481 tcaaccagct caacccattc ggtttgctca cgtagggtta cgcaatgctt tccaaagaaa
      541 aatgcctctt tctgtaaacc gccgctatcg gtaaccacca gcgaggattg gtttagcagc
      601 atgagcatat caaaatagcc taagggttca actatcctaa agttgggtga aatacttacc
      661 ctttcaagaa tttttgctgt tcggggatgt aggggaacaa ctacctgagt ttcagaattt
      721 atacggttca gcccatcaac tattccctga agattattaa gattatcggt gttttcctgt
      781 ctgtgaaagg tagccagcac aaagtgctcg ggtagattta agcgttcgag tacatgtgac
      841 tcggttttag ccctgttaaa gtaaaagtag gctgcatcct gcataacgtc gccactcctg
      901 ataattttgg cgttgaaatg gctgaatcct tcggccatca ggttgctgtc cgccgcttct
      961 gttggggtaa aaagtaaatc cgatatccgg tcagtaagaa tgcggttgat ctcctcgggc
     1021 atggtcatgt tgaacgagcg gagtcctgct tcaacgtgca ccacgcgggt gttcagcttt
     1081 ttggctacca gtgctccggc aagtgttgaa ttggtgtcgc cataaaccat tactgctgcc
     1141 ggtttttcat taataacgat gttttcgagc agctccatca ttctaccagt catggcgcca
     1201 tggttcaaac catgaacctc aaggttgtac tttggagcgg gaatgcacat ttccttaaaa
     1261 aatatctcgg acatgttttc atcgaaatgc tggccggtgt gcacaatcac ttcttccaat
     1321 ccaaaagcca taagttggcg gctaactgcg gctgccttta tgaactgggg tctagcccct
     1381 aaaacagtaa gtatcaactt gctattcatt agtcatttca tttaataggt ttgataaggt
     1441 gcgggtgagc tccttacggg agtaacgcaa aatatccttg gtctctggcc gcaggttgtt
     1501 ctctttatag agattgtaga gatgtgtaat gaccctttta gtttttgctt tatcgttata
     1561 gtccacaaag aatcctgctt ttgtctcgga aagcagggag gccaaatcgc cataggtggg
     1621 tccaactgcc agcaccattc gccccgaggc taagtactcg aatacctttc cggtaagaat
     1681 acccatagca tttggagaag aatttacaac caggagtaac acctgcgatt ttcgttgaaa
     1741 ttttaccgcc tcgacatggg taacctgtcc caaactttca aggttatttt gaaggtttac
     1801 tttttggatg gattctatag cagttgcatc tacctgtcct acgagcctga taaccagatc
     1861 atttctaaaa ccttcaattt cttcacataa atcggagagt acctcccata atatgacagg
     1921 gttaaagtaa cgggatagcg taccaacatg ggtaatcgag aattttgagt ccaggtcaac
     1981 cggctcattt ggccaatctt cctcatcaaa gccattggta atcagattta tacgtttagg
     2041 tctgagaacc ataagttcat ttttcatttg cgccgataca actatcaccc catcggcctc
     2101 acgaaccac
//
LOCUS       DAOOQX010000356         2107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_65356_length_2107_cov_3.454191, whole genome shotgun sequence.
ACCESSION   DAOOQX010000356 DAOOQX010000000
VERSION     DAOOQX010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65356_length_2107_cov_3.454191"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="PLX53_06290"
     CDS             complement(<1..1207)
                     /locus_tag="PLX53_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOY72728.1"
                     /translation="MERLTADTVKRTQLTGMYKNWFLDYASYVILERAVPHLDDGLKP
                     VQRRILHAMKRLDDGRYNKVANIIGYTMQYHPHGDASIGDALVQLGQKNLLVDVQGNW
                     GNILTGDGAAAPRYIEARLSKFANDVVFNPKTTEWMLSYDGRNQEPVTLPVKFPLLLA
                     QGVEGIAVGLASKILPHNFNELIDAAVDYLRGKEFEIYPDFPTGGMADCSRYNDGLRG
                     GSVKLRARIQKVDRKTLVITELPFGKTTSSLIESIVKASEKGKIKIKRIDDNTAGNVE
                     ILIHLPPEANPDMTIDAMYAFTDCEISISTNACVIYEDKPCFMGVKDILKASVERTKE
                     LLTRELNIRLGELEEEWHFSSLEKVFFEKRIYRELEKDTETWDMVIDAIERGFDPYRK
                     LFRRPITRDD"
     gene            complement(1308..>2107)
                     /locus_tag="PLX53_06295"
     CDS             complement(1308..>2107)
                     /locus_tag="PLX53_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOY72729.1"
                     /translation="KIARERAKKASLHNKKLRDCRVHYNTSDPRAEESTLFITEGDSA
                     SGSITKSRDVNTQAVFSLKGKPLNTYGLTKKIVYENEEFNLLQSALDIEDDMDNLRYN
                     RIVIATDADVDGMHIRLLLISFFLQFFPELIRQEHLFILQTPLFRVRNKKKTSYCYSS
                     EEREKAIRELGPNPEITRFKGLGEISPEEFANFIGPDMRLDPVRLTKDDQIFALLEFY
                     MGKNTPLRQDFIINNLRIEEDIVEEDTIHFEDGNVTAEASFQHDTLV"
BASE COUNT          463 a          508 c          551 g          585 t
ORIGIN      
        1 catcgtcgcg ggttattggt cggcggaaaa gtttcctata agggtcaaaa cctcgctcaa
       61 tggcgtcaat caccatatcc catgtttcgg tatccttctc cagctcacgg tatatccgct
      121 tttcaaagaa taccttctca agcgaggaga aatgccactc ctcttcaagt tcgcccaacc
      181 ggatgttcag ctcgcgggta agcagctcct tggtccgctc aaccgaagct tttagaatgt
      241 ccttaacccc catgaagcat ggtttgtcct cgtaaataac gcatgcgttg gtagaaatgg
      301 atatttcaca gtcggtaaag gcgtacatgg cgtcgatggt catatcggga ttggcctccg
      361 gcggaaggtg aatgagtatt tcaacatttc ccgccgtgtt gtcgtcaatt ctctttattt
      421 tgatttttcc cttttcgctg gccttaacaa tagattcgat gagcgatgag gtagttttgc
      481 caaagggtaa ctcggtgatg accaatgttt tccggtcaac tttttgaata cgggcacgca
      541 acttaaccga gccgcccctg aggccatcgt tgtaacggct gcaatcggcc atgccgcctg
      601 ttgggaaatc ggggtagatc tcaaattctt tacctctgag gtagtcaacg gctgcatcaa
      661 tcagctcgtt gaagttgtgg ggcagaattt ttgaggccaa tccgacggca atgccctcaa
      721 cgccttgtgc cagcaacagt ggaaacttta cggggagagt aacgggctcc tggtttctac
      781 cgtcgtagct gagcatccac tcggtggttt tagggttaaa caccacgtcg ttggcaaatt
      841 tcgacaggcg tgcctctatg taccggggag cagccgctcc atcacccgta aggatgttgc
      901 cccagttacc ctgaacgtca accagcaggt ttttttgccc cagttgaaca agcgcatcac
      961 caatggaggc gtcgccatgg ggatggtact gcatggtgta gccaattata ttggccacct
     1021 tgttgtagcg gccatcatca aggcgtttca tggcatgtag aatgcgacgc tgaacaggct
     1081 tcagcccgtc gtcaaggtga ggtacagccc gttccagaat aacgtacgag gcgtagtcaa
     1141 gaaaccagtt tttgtacatt ccggttagct gggttctttt taccgtatcg gcggttagcc
     1201 tttcaatttt ggcttgctct tcgggtgaag tatttttttc gccgccaacg agtagctcgt
     1261 cggcatcaaa atcgtcaagt cgcatggcgt gctgcagcgt ttgttagtta taccagggta
     1321 tcgtgctgaa agcttgcctc agccgtaaca ttcccatctt caaaatgaat agtatcctcc
     1381 tccactatat cctcttctat tctgaggtta ttgatgatga aatcctgacg taaaggggta
     1441 tttttaccca tgtagaattc gagcagggca aatatttggt cgtccttggt aagccgaacg
     1501 gggtcgagcc gcatgtcggg gccaatgaag ttggcaaatt cttcgggtga tatttcacca
     1561 aggcctttaa atcgggtaat ttcggggtta gggccaagtt ccctaatggc cttctcgcgc
     1621 tcttcggaag agtagcagta tgatgttttt ttcttattcc ttaccctgaa cagcggcgtt
     1681 tgaaggatga aaaggtgctc ctgccggatt agctcgggga agaattgcag gaaaaagctg
     1741 atgagcagta gccggatgtg catcccgtca acatcggcat cggtggctat cacaatgcgg
     1801 ttgtagcgga ggttgtccat gtcgtcctca atgtccagcg ccgattgcag caggttgaat
     1861 tcttcgttct cgtaaactat tttcttggtc aggccgtagg tgttgagggg tttccccttc
     1921 agggagaaaa cggcttgggt gttcacatcg cgcgacttgg taatggaacc gctggccgaa
     1981 tccccctcgg tgataaacag ggttgactcc tcggcacggg gatcgctggt gttgtagtga
     2041 acacggcaat cgcgcagctt cttgttatgc aggctggcct tttttgcccg ttcacgggca
     2101 atttttt
//
LOCUS       DAOOQX010000357         2104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_65515_length_2104_cov_2.370425, whole genome shotgun sequence.
ACCESSION   DAOOQX010000357 DAOOQX010000000
VERSION     DAOOQX010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65515_length_2104_cov_2.370425"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PLX53_06300"
     CDS             <1..492
                     /locus_tag="PLX53_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOY72730.1"
                     /translation="FKMQTRSVNIAVKSYAFGIPGIKVDGNDLLAMTSALQEASAHAR
                     AGNGPVLIEAFTYRRGSHTTSDDPTKYRSKEEEEAMAATDPIDRLRKYLIGQKLYDDS
                     SEGELISQFKQEVERQFGEAENYHPYPLEDVFKYTYVDMPDELKQQKIEHEKFLKWKE
                     SLK"
     gene            631..1599
                     /locus_tag="PLX53_06305"
     CDS             631..1599
                     /locus_tag="PLX53_06305"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOY72731.1"
                     /translation="MTMVQAINNALDIKLQEDPMIVTYGEDVGVEGGVFRATEGLQKK
                     HGTNRVFDSPLAESAIVGTAVGMAANGLKPIVELQFCGFVYPSYNQIISHVARFHNRS
                     RGRYKMPMVIRFPYGGGINALEHHSESMETIYGHIPGVKVVIPSTPYDAKGLLISAIE
                     SEDPIIFMEPKRIYRAIKQEVPEEKYSIPLGKAKVLQQGTDITLIAWGAMVREAQKAI
                     VMAKKDGIAVELIDLRSIYPIDRQTIVESVRKTGRVVIAHEEPLTFGTGAELTAIINE
                     EAFLSLEAPVNRVAGFDTIVPLPRGEHHYIISPEKIYYNILKTMKF"
     gene            1626..>2104
                     /locus_tag="PLX53_06310"
     CDS             1626..>2104
                     /locus_tag="PLX53_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HOY72732.1"
                     /translation="MRYIFNFPDIGEGLDEGTIAEWYVQKGQHVEAGDPLVNMETDKV
                     VTDIPSPKSGTIVATYGKIGETIRVGSPLVEIDIEGVEGEAAVAEVRKEEPVEEGAGV
                     VGTLEVAGNSAFLPAGDEGFEQQPAADSKIGRKALATPVARAMAKELGIDINRVTGTG
                     "
BASE COUNT          598 a          436 c          545 g          525 t
ORIGIN      
        1 tttaaaatgc aaacccggtc ggtcaacatt gcggttaaat catatgcttt tggtatccct
       61 ggtataaagg ttgatgggaa tgacctactt gccatgacta gtgcgctgca ggaagcctcg
      121 gcgcatgcca gggcaggtaa cggacccgta cttattgagg cgtttaccta tcgccgcggc
      181 tcccacacca cctccgatga cccaactaaa taccgttcca aggaggagga ggaggccatg
      241 gctgctaccg atcctattga ccggcttcga aagtatctta ttgggcaaaa gctttacgat
      301 gattcttccg agggtgagct gatatcccaa tttaagcaag aggttgaacg gcagtttggc
      361 gaggccgaaa actaccatcc atacccgctt gaggatgtgt ttaaatacac ctatgttgat
      421 atgcctgatg agctgaagca gcagaagatt gagcatgaaa aattccttaa atggaaggag
      481 agtttaaaat gaaatatccc aatggcaacg aattagagtt atagcctgtt gtgatagggg
      541 tatatcattc gggcaaccac cttttgatta caataagatt tggaataaat agattaacaa
      601 tcacttatac ggattaagca gatgaaagta atgacaatgg ttcaggcaat aaataatgcc
      661 ttggatataa agttacaaga agaccctatg atagttacct acggagaaga tgtaggtgtt
      721 gagggtggtg tatttagggc aaccgagggg ttacagaaga aacatgggac taacagggtt
      781 tttgattcac ctcttgccga atcggcaatt gtaggcactg ctgtggggat ggctgctaac
      841 gggcttaaac ctattgtgga actccagttt tgtgggtttg tttacccttc ctataaccag
      901 attattagcc acgtggccag gtttcacaac cgatcacgtg gtcgctacaa aatgcccatg
      961 gtgattcgct tcccttacgg tggtggaata aatgcgctgg agcatcactc cgaaagcatg
     1021 gagaccattt acggccatat tccgggagta aaagtggtta ttccctcaac tccttacgat
     1081 gcaaaggggc ttctaatttc ggctatcgaa agtgaagatc ccattatctt catggaaccc
     1141 aagcgaattt accgggccat taagcaggag gttcccgagg aaaaatacag cattccattg
     1201 gggaaggcca aagttcttca gcagggaaca gatatcactt tgattgcctg gggagccatg
     1261 gtacgcgagg ctcaaaaagc aattgttatg gctaaaaagg atggaattgc agtggagctc
     1321 atcgacctcc ggtccattta tccgattgac aggcaaacca tcgtggaatc cgtccggaag
     1381 accggtaggg tggtaatagc ccatgaggag ccgcttacct ttggaacagg cgcagagctt
     1441 accgccatta ttaacgaaga ggccttccta agccttgaag ctccggttaa ccgtgttgca
     1501 ggttttgaca ccatagtgcc cctgcctcgt ggcgaacatc actatatcat ttcacccgaa
     1561 aagatatact acaatatcct aaaaacgatg aaattttaaa tgcttgaaag tttaaactac
     1621 taaacatgag gtatatattt aattttccgg atattggcga aggccttgac gaaggaacaa
     1681 ttgccgagtg gtatgtgcag aagggacaac atgttgaagc cggcgacccg cttgttaaca
     1741 tggaaaccga taaggtggta accgatattc cttctcccaa atcgggaacc attgttgcca
     1801 cctacggtaa gattggagaa actatacgtg tgggctcccc gcttgttgag attgatattg
     1861 agggcgttga gggagaagca gcagttgctg aggtccgaaa agaagaacct gttgaggagg
     1921 gtgcaggggt tgttggtacc cttgaggttg ccggaaatag cgcctttttg cctgcaggag
     1981 acgaaggttt tgaacaacaa cctgctgcag atagtaaaat tgggcgcaag gctctagcaa
     2041 cgccagttgc tcgggcaatg gcaaaggagt tgggtataga tatcaatagg gttaccggga
     2101 ctgg
//
LOCUS       DAOOQX010000358         2101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_65662_length_2101_cov_2.412023, whole genome shotgun sequence.
ACCESSION   DAOOQX010000358 DAOOQX010000000
VERSION     DAOOQX010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65662_length_2101_cov_2.412023"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PLX53_06315"
     CDS             complement(<1..520)
                     /locus_tag="PLX53_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY72733.1"
                     /translation="MRDLRSKISNSQYIILNLLETPILAFILAYVVRYIADPNSKIYI
                     FRENENIPIYIFMALIVALFIGLTVSAEEIFRDRKILKREAFLNLSRSSYLLSKVAIL
                     FLLSAIQSATFILIANSILEIRDMYFDYWLVLFTTAAAANLLGLNISATFNSAVTIYI
                     VIPLVMIPMMVLS"
     gene            complement(811..1383)
                     /locus_tag="PLX53_06320"
                     /pseudo
     CDS             complement(811..1383)
                     /locus_tag="PLX53_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          515 a          442 c          557 g          587 t
ORIGIN      
        1 ctgaaagcac catcatgggg atcatcacca ggggtattac tatgtaaata gttacagcag
       61 agttgaaggt ggccgaaatg ttcaacccta gcaggttagc tgctgcagcc gtggtgaata
      121 gcaccagcca gtaatcgaag tacatgtccc tgatttcgag aatgctgttg gcgattagga
      181 taaaggtagc ggattgaatg gctgatagca ggaataaaat ggcgaccttg gaaagcaggt
      241 agctcgaccg gctaaggttg aggaatgcct ctcgcttgag tatttttcgg tcgcggaaaa
      301 tttcttcggc gctaacggta agtccaatga acaacgctac aataagagcc atgaagatgt
      361 agatgggaat attctcattt tccctgaaga tgtaaatctt agagttggga tcggcaatgt
      421 agcgcaccac gtaggccagg atgaaggcaa gaattggggt ctcaagcagg ttaaggatga
      481 tgtactggct attgctaatt tttgaacgca ggtcgcgcat ggtgtagatg gtgaactgct
      541 taaagcgcga ggggatgttg agggttcgtg gtggttgacc ttttcgtcgg gaataactgg
      601 aggcttgatg ttgctggcga aatgttcctc ccatttctgg ggtgaaacct ttcgtatggg
      661 ggtgtagcgg ccaaactcat caaccactct tgcctctata atgttgaata gcagctcggg
      721 gttaacatta ccgcaaaccg ggcattctcc aacctcgctg tttatttgca tgtcgaggcg
      781 tttgaaatac atcacagctt caaccggatt gccgtagtag atcatgtagc ccccggtatc
      841 gaggatgatc atgttgtcga acattttgta aatgtccgaa gagggttggt ggatgaccac
      901 gaaaataagt tttcctctaa gggtaagttc cctcagcagg tccataacat tttccgagtc
      961 gcgcgatgaa agccccgagg tgggttcatc cacaaacatg attgagggtt cgcggattag
     1021 ctccagggca atgttaagcc gctttcgctg ccctcccgaa atcatcttgt tcaggggggt
     1081 gccaaccttt aaattctttt gctcgagcaa gcctaggttc gatagggttt tatcaaccat
     1141 ggcgataagc tcaggttcgg tcttatcctt aaagcaaagt tttgcgttgt agtatagatt
     1201 ttcaaaaact gtaagctcct caattagcag gtcgtcctgc gggataacgc caataacacc
     1261 ttcgagcata ttgctactgg cgtgtaggtc gtagccgttg atgtaaacat ggcctgaagt
     1321 aggcttttcg atgcctgcca gcacgttaag cagagtggtt ttcccggcac cactggcgcc
     1381 cataattcct atcagcttac catgaccctc gctgaagctg atgttgtgca ggccaacggt
     1441 tccgttcttg aaggtgtagc ttgcattgtc caccctgtag gatattctga tagtagtgga
     1501 gtcagaaagg aagtgggcca ccacgtcggt gtagtacacg ggcttgccct tgggcagctt
     1561 cagggagcta ccgcttgcaa acaggtagat acgacggttg ctgatgggta gcccgttcag
     1621 gtaaatatcc tcattcccgg tatagcgtaa aaagtaaagg tcctcgcttt tgatttgaag
     1681 gatataaata aatccgtcga gagcttcggt ggggatatgc ttgcactgtt cgcccttaaa
     1741 aggtctgtcg tgaatggtta gtatgctggg tcggtctaac tcctcgggat tattccttac
     1801 tacaaagttt tctatgtcgt taatctcttc gtgcgaaagt ttgaaaactt cagcaacggt
     1861 gttaataatg gccatgcgtt gttcggtaaa cttacggtcg gcattgataa gctcgtagag
     1921 gcgaaccaga acaactattt tttgtttttg ggtaagagtt ttgttaattt tcttgcagat
     1981 gccgaggatg cgaacggaat cctttaccga tgtgagttta ggttttgacc tttcctcgtc
     2041 gtcggaccct atctccttgt ctaaaaggcc tgcgtgctcg tcgaaaagcc taaggtattc
     2101 t
//
LOCUS       DAOOQX010000359         2087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_66321_length_2087_cov_2.624016, whole genome shotgun sequence.
ACCESSION   DAOOQX010000359 DAOOQX010000000
VERSION     DAOOQX010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66321_length_2087_cov_2.624016"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="PLX53_06325"
     CDS             <1..1475
                     /locus_tag="PLX53_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY72734.1"
                     /translation="QDADGTFLDVNKACEKYFGYQREYFIGKKPDDLSAPGKNDIEMV
                     AACIQKAYNGQPQIFEFWGRRSDGFIFPKEVSLTPGEYFGRKVVIAVSRDITEQKNWE
                     AELIAAREKAEESDRLKSAFLANMSHEIRTPMNGILGFAQLLKNPRLSEEEVREYLDV
                     IEQSGQRMLDIINNLINISKIESGEVQVNLSQCDVNEQLQYALNFFKPDADSKGLKFF
                     LEYQLPDEQATVTTDPLKLQVILSNLVKNAIKYCDNGSVAFGCKLKEEYLEFYVSDTG
                     IGIPPLTIGKIFERFVQANTNYSGRYEGAGLGLAIAKSYVELLGGSIWVESEVGKGST
                     FHFTIPVRPAARKQQSYSNALNQDKNDSVSQPFSKKLKILIAEDDAPSGMLLRNQLKD
                     IAGEIISARNGADAVSMFLSNPDTDLILMDIKMPEMDGYEATKKIREVSSNVVIIAET
                     AYAMTNDREKALSVGCNDYISKPINREKLLELVKKHFELD"
     gene            complement(1499..>2087)
                     /locus_tag="PLX53_06330"
     CDS             complement(1499..>2087)
                     /locus_tag="PLX53_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOY72735.1"
                     /translation="QELGRKWYEDGRKLCKMNTFTDFIACTECLVEQKYTSPDRIVAQ
                     GASAGGLLMGVIANLRPDLYRAIVAEVPFVDVLTTMLNPNLPLTTGEYDEWGNPNDSI
                     VYHYIKSYSPYDGVRQQDYPSMLVLASYYDSQVQYFEPAKWVAKLRASKTDNNLLLLK
                     TDMESGHAGSLGRYKALKDEAFVYAFILHAIGIED"
BASE COUNT          605 a          432 c          513 g          537 t
ORIGIN      
        1 ttcaggatgc cgatggcacc ttcctggatg taaataaggc ctgcgaaaaa tactttggat
       61 accagcggga gtactttatc gggaaaaagc ctgatgatct gtctgccccg ggtaaaaacg
      121 acatagaaat ggtggcagct tgtatccaaa aggcgtacaa cggacaaccc caaatctttg
      181 aattctgggg acgtcgtagc gatggtttca ttttccctaa agaggtaagc ttaactcccg
      241 gcgagtactt cggtagaaaa gtggtgatag ctgtttccag ggacatcacc gagcaaaaga
      301 attgggaggc tgagctaata gccgcacgtg agaaggcgga ggagagcgat aggctaaaat
      361 cggcattttt ggccaacatg agccacgaaa ttcgaacccc gatgaacgga atacttggtt
      421 ttgcccagct actaaaaaac ccgagactct cggaagagga ggtaagggaa taccttgacg
      481 ttattgagca gagcggtcag cgaatgctcg atataatcaa caaccttatc aacatttcta
      541 aaattgagtc gggtgaagtg caggtgaacc taagccaatg cgacgtaaac gagcagctgc
      601 agtatgcgtt gaactttttt aagcccgacg ccgatagtaa ggggctaaaa ttttttctgg
      661 aataccagct acctgatgag caagccactg ttactaccga tccgttaaag ttgcaggtaa
      721 ttctatcgaa cctagtcaaa aacgccataa agtattgcga taacggaagt gtagcgtttg
      781 gttgcaaatt aaaggaggag tacctggaat tttacgtgag tgacacaggc atcggaattc
      841 cccctctgac tatcggaaag atatttgagc ggttcgttca ggctaacacc aactactctg
      901 gtaggtacga gggtgctgga ttggggctcg ccattgcaaa atcgtatgta gagctccttg
      961 gggggagtat ttgggtggaa agcgaagtag ggaaaggttc aactttccat tttaccatac
     1021 cggttcggcc tgctgctcgt aagcagcaat cttattctaa tgctttaaat caggataaaa
     1081 acgattcggt aagtcaacca tttagtaaaa agttgaaaat tcttatagcc gaggatgatg
     1141 ccccttcggg aatgctactt agaaaccagc tgaaggatat tgcaggtgaa ataatatcgg
     1201 ccaggaatgg tgccgatgct gtgagcatgt ttcttagtaa ccccgatacc gatctaatac
     1261 ttatggatat taaaatgcca gaaatggatg gctatgaagc aaccaaaaaa attagagagg
     1321 taagctccaa cgtggttatt attgccgaaa cggcctatgc catgacaaac gacagggaga
     1381 aagcccttag cgtgggatgt aatgattaca tttccaaacc cataaaccgg gaaaaattac
     1441 tggagcttgt aaaaaaacac tttgaacttg actagctgga agcgtcttta gagtgattct
     1501 agtcctcaat tcctattgcg tgaagtataa atgcgtacac aaaagcttca tcttttagag
     1561 ctttgtacct gcccaaacta cccgcatgac ccgattccat atctgttttt agaagtagga
     1621 ggttgttatc tgttttgctg gctctcaact tggctaccca cttggcgggt tcaaaatact
     1681 gaacttgaga atcatagtag ctggctagaa caagcatgct agggtaatcc tgctgcctaa
     1741 cgccatcgta aggggagtat gattttatat agtggtaaac tatggaatcg tttgggttgc
     1801 cccattcatc atattctcca gttgtgagcg gtaggttagg attaagcatg gtggtaagca
     1861 cgtcaacaaa ggggacttca gctacaattg ccctgtataa atcgggacga aggttggcaa
     1921 taacccccat caggaggcct ccggcgcttg cgccctgtgc tactatccta tcaggcgagg
     1981 tgtatttctg ctcaacaagg cattcggtgc aggcaatgaa gtcggtaaat gtgttcattt
     2041 tgcataactt cctgccatct tcataccatt tgcgacccag ctcttgt
//
LOCUS       DAOOQX010000360         2085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_66422_length_2085_cov_2.820690, whole genome shotgun sequence.
ACCESSION   DAOOQX010000360 DAOOQX010000000
VERSION     DAOOQX010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66422_length_2085_cov_2.820690"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1646
                     /locus_tag="PLX53_06335"
     CDS             222..1646
                     /locus_tag="PLX53_06335"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOY72736.1"
                     /translation="MNFKMLLKNVLSIKNGKPLAFLLMLLFLPVFVFASEGGSEAPSI
                     GGIRVEFILFGLTLIGVALFHKQTFWVAICGLTAIVLYKLLFISGYPFAEHFFGENAM
                     VDQILHKDLRQGEWGILVNLLGLLLGFAILAKIFEESGVPDILPRFLPSGWQGPFVLL
                     IFIFILSSFLDNIAAAMIGGTIALVVFKNKVHVGYIAAIVAASNAGGAGSVVGDTTTT
                     MMWIDGVSALNVLHAYVAAGVALIILAWFASHQQDHHQQIVKDAKTDVKIDWVRLGIV
                     VLILAGAILSNIYYDMPALGVWIAILIGAIFTKIPWQEVGASIKGTIFLLCLVTSASF
                     MPVETLPDASWMTAFILGFVSAVFDNIPLTKLCLDQGHYDWGMLAYAVGFGGSMIWFG
                     SSAGVAITNKFQEAKNVTLWLRKGWHVTLAYIVGFFTLYITMGWEPASNKQHQEPAIE
                     CKAPDCPVRAKAEFERTLHPDEVK"
BASE COUNT          526 a          446 c          477 g          636 t
ORIGIN      
        1 caaccacctc tacgccgaag gtggagatat tttgtcccag ctgaatttgc ttgtgtgaag
       61 accatactgt tagatattcc catgtatttt gacctctaaa atgccttaaa aaaacaacat
      121 ttgctaaaat tatctattac caaccgcaaa agctggatta ttttcattga tttgttaaat
      181 ttgacgcatt cctaacctaa aattaacttt aaactaaact tatgaatttc aagatgttac
      241 taaagaatgt cttgagcata aagaacggta aaccgttggc atttctgctc atgctgctgt
      301 ttttgcctgt ttttgttttc gcctccgagg gaggaagtga ggcaccttct attggtggaa
      361 tccgggttga atttatactg tttggcctaa cccttatagg agttgccctt tttcataaac
      421 aaaccttttg ggtggcaata tgcggattaa cggcaattgt gctctacaag ctgctattta
      481 tttccgggta tccttttgcc gagcactttt tcggagaaaa tgcgatggtt gaccagatat
      541 tgcacaagga cctgcgtcag ggggaatggg gtattctggt taacctgctg ggattgttgc
      601 tgggatttgc catactggca aaaatttttg aggaatcggg cgtgccggat atactaccca
      661 ggtttcttcc aagtggttgg cagggacctt ttgtactgct tattttcatc ttcatcctct
      721 cgtcattcct cgataacatt gccgctgcaa tgattggtgg cacaattgcc ctggtagttt
      781 tcaagaataa agtacatgtt ggctatatag cggcaattgt tgctgcttca aatgcaggag
      841 gtgcaggtag cgtggttggc gacaccacca ccaccatgat gtggattgac ggggtttcag
      901 cacttaatgt actacatgcc tacgtagctg ccggggttgc ccttataatt ctggcatggt
      961 ttgcttcaca ccagcaggac caccatcagc aaattgttaa ggatgcaaag actgatgtaa
     1021 agattgactg ggttcgcctg ggaatagtgg tgcttattct tgccggtgcc attctctcga
     1081 acatatacta cgatatgcct gcccttgggg tgtggattgc cattcttatt ggggcaattt
     1141 ttaccaaaat tccctggcag gaggttggag catccattaa gggaaccatt tttctattgt
     1201 gcctggtaac ctccgcctcg ttcatgccgg ttgagaccct tcccgatgct tcatggatga
     1261 ccgcatttat tctggggttt gtttcggctg tttttgacaa catcccgcta acaaagctct
     1321 gccttgacca ggggcattac gattggggca tgttggccta cgccgtgggc tttggaggtt
     1381 ctatgatttg gtttggttcg tcggctggcg tggctataac caataagttc caggaggcta
     1441 aaaatgtaac gctatggtta cgaaaaggct ggcacgttac cctggcctat attgtcggtt
     1501 tctttaccct atacatcacc atgggatggg aacctgccag caacaagcag caccaggaac
     1561 ctgcaattga gtgtaaggca cccgactgtc cggttagggc aaaagctgaa tttgagagaa
     1621 cattgcaccc cgatgaggtt aaatgatctg taactccgga atagagaaaa cactttgtgc
     1681 tttagctatt aaattttata agtgaagaag gtgctaccag ctagaaattt tagctaaatt
     1741 aaaaaacaga ggaattgctg aaataaaagt caaccctttt ggttgatttt cctaaaagcg
     1801 aaaaaacctg ccccccacat agatggttcc ggcaggtttt ttcgctattt ccttagtaag
     1861 ctatatgggc agaaaaaatt atggtttgcc caacccggct agaattgttc ttaccctggc
     1921 gcaaagctcc aactaccttc ttacagcttt aaaacactgg taacccgttc ctgcttacgg
     1981 tctagtaatt ttttcattaa tcctgctgta tcctcaaagc ggttagcaag catcatggct
     2041 gcaattatgt agggcttata tcccgcctca aggtaggtct ttatg
//
LOCUS       DAOOQX010000361         2084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_66473_length_2084_cov_2.034007, whole genome shotgun sequence.
ACCESSION   DAOOQX010000361 DAOOQX010000000
VERSION     DAOOQX010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66473_length_2084_cov_2.034007"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..855)
                     /locus_tag="PLX53_06340"
                     /pseudo
     CDS             complement(1..855)
                     /locus_tag="PLX53_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
     gene            1197..1922
                     /locus_tag="PLX53_06345"
     CDS             1197..1922
                     /locus_tag="PLX53_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOY72737.1"
                     /translation="MVKVLESQHVASILKKMPLSKRGKVKEVTLDMASTMQKIVRQRF
                     PNAEQVADRFHVQQLVYEAVQDLRIHHRWKAIEQENAEMELCKELDQEYKPNILENGD
                     TLRQLLARSRNLLFKQENKWTLSQYQRAEILFKLFPDIKLANELCRELSSIYGSTKDK
                     EVAFTRLARWYEKVERAGFKTFNTVSRTVQNNSLTILNYFNNRSTNASAESFNAKIKA
                     FRSQFRGVANIPFFLFRLANIYA"
BASE COUNT          667 a          492 c          389 g          536 t
ORIGIN      
        1 tattactcca ccactaccgc caactttaac aagtttattg ctaacaacca gtgatgcagc
       61 atcgttcaaa ctcagcccct tatactccat caatgctgaa atatcatggg ctacggtata
      121 gcgtataaaa aattctccat ggccggtagc cgaaatagca caggttgcat tattagcata
      181 agttccagcc ccaataattg gagcatctcc tacccttccc catcgcttgt tggtcattcc
      241 tcctgtggaa gttgctgccg caagatttcc attaacatcg cgtgcaacgc accccactgt
      301 tcccattttt tcatgctcca gcacacgttg gaggtcatcc caccagggtt tagtataaaa
      361 atagccgcta tcgaccatag ttgcaccatg caggcgagca aattccgaag ctcccttccc
      421 taccaataat acgtggggag atttgagcat tacaagcttg gctaattata ttgggttctt
      481 cacatcgcca acacccgcaa cagcgccagc agccaagttg ctaccatcca tcattgcagc
      541 gtcaagctca ttatgcccat catgggtaaa aacggcaccc ttaccggcat taaaaagcgg
      601 acaatcctca aggtacctga ctacttgctc aaccgcatca agggatgaac cgccgccaga
      661 caaaatgcgt ttacctatat cgagcgcatt ctttaattca ttctgatagg ttgcgagctg
      721 ttcaggagtg aaatctgctg gttccattcc acctgcaccg ccatgcagaa caatactcca
      781 gttctgggaa aacccaatgg atgacagtaa aagtaaggcg gtagcaacaa gtaaaacttt
      841 acatttctgc ttcattctaa aataaattat tgctttgcac tcatcattgc cattttctct
      901 tcatcaacaa acatataaaa tttaaaaaga tcattcccgt cactctattt cgtacttact
      961 accaagctat aatttttgcc aagggaaata cttttcaaac tctacgggaa aattcaccag
     1021 ctaaaaaggt taatcaccac atcaatagcc ataaaacttc tatcaaattg aaaacatcca
     1081 aaactttcaa accttgtaaa tgtagtaaat tttaaaaatt tgaattgaaa ccatcataag
     1141 attatagtta aaaacaaaaa cattaaccca gaaaagggtt aaaattaaga gactaaatgg
     1201 taaaagtttt agagagccag catgttgctt caatactaaa aaagatgccc ctaagcaaaa
     1261 ggggtaaagt taaggaggtg acgttggata tggcttcaac catgcaaaag attgtcaggc
     1321 agcgcttccc gaatgccgaa caggtagctg accgcttcca tgttcagcag ctggtctatg
     1381 aagccgtgca ggacttgcgg attcaccacc ggtggaaagc gatagagcag gaaaatgccg
     1441 agatggagct atgcaaagag cttgatcagg agtacaagcc taatatcctt gaaaatgggg
     1501 atactttaag gcagctgcta gccaggagca ggaacctgct tttcaagcag gaaaacaagt
     1561 ggaccctatc gcaataccaa cgggcagaaa tcctgttcaa gctcttccct gatattaagc
     1621 tcgcaaacga attatgccgt gagctaagca gtatttatgg gagtacaaag gataaagagg
     1681 tggcttttac acgcttggca agatggtatg aaaaagtaga aagggcaggg tttaaaacat
     1741 ttaatactgt gtcaagaacc gtacagaaca actccctgac aatactgaac tatttcaaca
     1801 acagaagtac aaatgcctcg gcagaatctt tcaacgccaa aatcaaggcc tttagatctc
     1861 aattcagagg agtggcaaat atcccattct tcctgtttag actggcaaat atttatgcgt
     1921 aaatatgaaa tccccagaaa ttttgcttga tccgatttac acactacggt acagtgccat
     1981 gcccggcaca ccaatatatt tcaggcggtt ttaaccgcct gaaattttat tctaacaatt
     2041 gcagaatttt tgtccctttt ctcaacctac ttccctaaca taac
//
LOCUS       DAOOQX010000362         2082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_66535_length_2082_cov_3.430686, whole genome shotgun sequence.
ACCESSION   DAOOQX010000362 DAOOQX010000000
VERSION     DAOOQX010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66535_length_2082_cov_3.430686"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1002
                     /locus_tag="PLX53_06350"
     CDS             121..1002
                     /locus_tag="PLX53_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72738.1"
                     /translation="MRKKYMQFTTTITGEQVENFCKSTADENPIHNQQHMRELGKEIV
                     VPGLYIFSLIVNFLQKNESLNFNTFRIFFGNIVGINEAIDLGFERSPISESQRYLFAR
                     TGADCFSLDNSRSFADIRSNGIGMEERGRHVPITYNKEQVHIFRKLLGLSNAALLSFL
                     FSVSLASPMLISTIKQPVTDVDKEIAMLFNKSVYPEGISPLYQSLEIFRLENSTILEP
                     EGQVDYWVQLEKEKAGKFYAANVSCGQSGKVMYNSRYRLVAVPNKVMLRMVRELKNQG
                     SKKVEEAKTLNSGRTLA"
     gene            complement(968..>2082)
                     /locus_tag="PLX53_06355"
     CDS             complement(968..>2082)
                     /locus_tag="PLX53_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY72739.1"
                     /translation="LMQFKPDVHNTLEGGLSSNLVNLNYFESYFHNVNGLSIIDTTHS
                     SDNLSALYSGWLQNTFAGGKLKALTIGFRATYHNLSGKFYFEPRLQAIVAPTPNLSLK
                     LAAGIYNQYVNRIVQANSSGVYRNFWIVSDGKLFPVVKSQHLIAGMTWEDKGWLVDVE
                     GYLKYTEGISFIQNVTRRVNGNQFKTTSLLLTLDSRATGVDLLVRKDWSKGQVWAAYS
                     LSRSFNESPKLNGGEEYYALDDHLHELKIAGTFTVGKWHLSASWIYGSPKPWDEILLT
                     SSLQLSPDYVKNSVRLIPYHRLDAGLAFVQRVDKVEVTAGVKAFNLYNRQNVIAKPFS
                     LSDTPVQDYLAGRALITFQEIIGMDFTPMFYLNLKF"
BASE COUNT          603 a          451 c          472 g          556 t
ORIGIN      
        1 aggaaaaaca cgataaacta cctggctcgg atgttacccg aaaggcaata tcgttacaat
       61 gttggctcct tagttgaaaa aagttaaaac gtttgtttaa cttgtaaaac ttaggtagtt
      121 ttgagaaaaa aatatatgca attcactact actataaccg gggagcaggt tgaaaacttt
      181 tgtaagtcaa ctgccgacga aaatcccatt cacaaccagc agcacatgcg agaactcggc
      241 aaggaaatag ttgttccagg cttatatatt ttctccctga tagtcaactt tctacaaaag
      301 aatgagtcct tgaattttaa tacatttcga attttcttcg gcaacatagt tggaattaac
      361 gaagctatcg atcttggttt tgagcgttcg cccataagcg aatcgcagag gtatctgttt
      421 gccagaaccg gtgctgattg tttttcgctc gacaacagca ggtcgtttgc cgatattcgt
      481 tcaaatggaa taggtatgga ggagcggggc cggcacgttc ccattaccta taacaaggag
      541 caagtgcata tatttcgaaa gctattaggg ctttcaaatg ccgctctact aagtttcctt
      601 ttctctgtat cgttggccag ccccatgctg ataagcacga taaaacaacc ggtaacagat
      661 gttgacaagg aaatagccat gttgttcaac aagtcggtgt acccagaggg aatttcgccg
      721 ctataccaat cactcgagat atttaggctg gaaaattcaa ccattctgga acctgaaggt
      781 caagttgact actgggttca gcttgaaaag gaaaaggccg gaaagtttta tgcggccaac
      841 gttagctgtg gacaaagcgg caaggtgatg tacaactccc gatacaggct ggtagcggtt
      901 cccaacaagg tgatgctaag gatggtcaga gagctgaaaa atcagggtag taaaaaggtt
      961 gaagaagcta aaactttaaa ttcaggtaga acattggcgt aaaatccatt ccaataattt
     1021 cctgaaacgt aattaatgcc cttcctgcca ggtaatcctg aacgggggta tcggacaggg
     1081 agaagggttt agctattacg ttttgcctat tgtagaggtt gaaagccttt acaccagctg
     1141 ttacctcaac tttgtcaact cgttgaacaa aggcaagccc ggcatccagc ctatggtagg
     1201 gtattagcct aacagaattc ttcacatagt cgggcgaaag ctgcaggctg gatgttagaa
     1261 gaatttcgtc ccagggtttt ggagatccat aaatccagct ggctgacagg tgccacttgc
     1321 cgacagtaaa tgttccggca attttaagtt catgcaggtg atcatccaga gcataatact
     1381 cctctcctcc gttcagtttt ggagattcat tgaaggaacg gctgagcgag taagctgccc
     1441 acacctgccc cttactccaa tccttcctga ccagcaaatc aactcccgtt gccctactgt
     1501 cgagtgtaag tagcaaggag gttgttttaa actggttccc attcacccgc cgggtaacat
     1561 tttgtataaa agaaatgccc tcggtgtatt tcaggtatcc ttcaacatct accagccatc
     1621 ctttgtcctc ccatgtcata ccagcaatca aatgttgcga tttcaccaca ggaaacaatt
     1681 taccatctga aactatccag aaattccggt aaacgcccga tgagttggct tgcacaattc
     1741 tatttacata ctgattgtaa atgcctgcgg ccaacttaag gcttaaatta ggtgtgggtg
     1801 caactatggc ctgcagtctg ggttcaaagt agaatttacc cgagaggttg tggtaagtgg
     1861 ccctaaaacc tatggttaaa gcttttagct tgccgccagc aaaggtgttc tgaagccaac
     1921 cggagtataa cgccgagagg ttatcagaac tatgggtggt atcgatgatg cttaacccat
     1981 tcacattgtg gaaatacgat tcaaagtagt tcaggttgac caggttagaa ctcaacccgc
     2041 cctcaagggt attatgcacg tcgggtttaa actgcatgag ca
//
LOCUS       DAOOQX010000363         2081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_66577_length_2081_cov_3.468904, whole genome shotgun sequence.
ACCESSION   DAOOQX010000363 DAOOQX010000000
VERSION     DAOOQX010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66577_length_2081_cov_3.468904"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLX53_06360"
     CDS             <1..182
                     /locus_tag="PLX53_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72740.1"
                     /translation="FFGSFLYQDKKEHLAGSLPQFRHGDEKNPNSSLEQLRFASPTHP
                     LQALHFKTIPPLQGL"
     gene            complement(317..>2081)
                     /locus_tag="PLX53_06365"
     CDS             complement(317..>2081)
                     /locus_tag="PLX53_06365"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY72741.1"
                     /translation="FWLSLIEAFSATNPLLIREVKRFVAENASIKLSQKPLNGKQVAI
                     VGAGPSGLTCAYYLAQAGFKVDVYESESKAGGMVRYAIPGFRLTSEALERDIARILQL
                     GVTIAYNHRITSEEFSKLKSTYDFLYISAGAQKATPLNIDGIEAAGVYDSLAFLKDVK
                     AGNIKGVGKNVVVIGGGNTAMDAARTAHRLVGTNGKVTVVYRRTIDEMPADEGEIKAV
                     MAEGAEIVELSAPERLIIEKGNVCGLVCSRMKLEGTDAKGRPKPVRVNNSEFTIPCDT
                     VIPAIGQEVDIDFVPKELLRAHTTTYKTLLHNVYIGGDALRGAATAIKAIGDGRKVAE
                     QIINQAGIQRSIKTPTGRSHSKEELIMKRAKRSYAPELPELSPSESRTFSLVSQTLDE
                     QSIVSESGRCLQCDEICNICTTVCPNLANFSYDISPVKYNMQRVLVGEDGQLKFEPDG
                     VFEVKQQYQILNIANFCNECGNCATFCPTSSAPYLNKPKLHLTTKSFNSSDEGYMLSK
                     LEDRNVLIYKNEGGIKTLSETAGELIYETDHVAARFDHDTFELKEVNVKTPCVRQIQF
                     QHAAEMSIILKGAKARIKDKG"
BASE COUNT          497 a          524 c          439 g          621 t
ORIGIN      
        1 ttttctttgg ttctttcttg tatcaagaca agaaagaaca cttagctggc tctttgccac
       61 aattccgcca tggtgatgaa aagaatccaa acagctcttt ggagcagcta cgatttgcat
      121 cgccaacaca ccctctccag gctcttcatt tcaaaactat tccacccctt cagggcttgt
      181 aaaaaatcgt tcgctccgta caaggggctg cgccccttgc tacttattac gccccttcgg
      241 ggcttaaatc cgtcaggtta ccgacaagtt ccgccaggat gcggacaagt tattgaatta
      301 acttaaccta aacctcttat cctttatcct ttatccttgc ttttgctccc ttcagaatga
      361 tactcatttc ggcagcatgc tggaattgga tttgcctaac acatggagtt tttacattaa
      421 cctccttaag ttcaaatgta tcatggtcaa atctggcagc tacatgatcg gtttcatata
      481 ttagttcacc agctgtttcg gaaagggttt taatccctcc ttcatttttg tatatcaaca
      541 catttctatc ttccagtttt gagagcatgt agccctcatc gctgctattg aacgacttgg
      601 tagtgaggtg aagttttggc ttgttaaggt agggtgcgct gctggtagga caaaaggttg
      661 cacagttgcc acactcgtta cagaagttgg caatgtttaa aatttgatac tgctgcttaa
      721 cctcaaacac cccatcgggt tcaaacttta gctgtccatc ttcgccaacc aacacccttt
      781 gcatgttgta ctttacgggc gaaatatcgt aggagaaatt ggccaggttg gggcacaccg
      841 ttgtgcagat gttgcaaatc tcatcgcact gaaggcacct gcctgattct gaaacaattg
      901 attgttcgtc aagagtttgg ctaaccaggc tgaatgtacg actctcggat ggtgatagtt
      961 ctggaagctc aggggcatac gaccgttttg cccttttcat aatgagctcc tctttagaat
     1021 ggctcctgcc ggttggagtt tttatgcttc tttgaatacc ggcctggttg attatttgct
     1081 ctgctacttt ccgtccatca ccaatggcct tgattgccgt agcagctccg cgtagggcat
     1141 caccgccaat gtaaacatta tgtagcagcg ttttataggt agttgtatgg gcccgtagga
     1201 gttctttggg gacaaaatca atatctacct cctggcctat agccggaata accgtatcgc
     1261 agggtatggt aaattcggag ttattcaccc taaccggctt ggggcgaccc tttgcatcgg
     1321 ttccctcaag tttcattcgg ctacatacca acccgcaaac gttacccttt tctataataa
     1381 gcctttcggg agctgacagc tcaacaattt cggctccctc agccatcacc gctttaatct
     1441 caccttcatc agccggcatt tcgtcaatgg ttcgtctgta aacgacggtt accttgccgt
     1501 tggttcctac cagcctatga gcagttcgag cagcatccat tgcggtgttt ccaccgccga
     1561 ttacaaccac attttttccc actcccttga tgttccctgc tttaacatcc ttcagaaaag
     1621 ctaacgagtc gtaaactccg gctgcctcta ttccatcaat attcaaaggg gttgcttttt
     1681 gtgctcctgc agaaatgtaa aggaaatcgt acgtgctttt tagcttggaa aattcctcgc
     1741 tggtaatgcg atggttatat gcaatagtaa cacccagctg caggatgcgc gcaatgtcgc
     1801 gctcaagagc ctcgctggtt aatcggaagc caggaatggc gtatcgaacc attccgccgg
     1861 ctttagattc cgattcgtaa acatcaactt taaaaccagc ttgcgccagg tagtaggcgc
     1921 acgttagtcc tgatgggccc gctccaacaa tggctacctg ctttccgttc aaaggctttt
     1981 ggcttagctt aattgaggca ttctcagcaa caaatcgttt tacctccctg attagcaaag
     2041 gatttgttgc tgagaatgcc tcaattaagc taagccaaaa g
//
LOCUS       DAOOQX010000364         2079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_66681_length_2079_cov_3.374012, whole genome shotgun sequence.
ACCESSION   DAOOQX010000364 DAOOQX010000000
VERSION     DAOOQX010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66681_length_2079_cov_3.374012"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PLX53_06370"
     CDS             complement(<1..643)
                     /locus_tag="PLX53_06370"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOY72742.1"
                     /translation="MNNFEFFNPVRILFGNDKIEKIGMQLNPTDRILMTYGGGSIKKN
                     GTYERIKAALSNFSVFEFGGIEANPKYETLMKAVEVVKKNNVDFILAIGGGSVIDGTK
                     FIAAASLYEGSDPWDLLIKNIVVTKAIPFGVVLTLPATGSEMNSGAVISRLETREKYS
                     FGSPVLFPKFSVLDPTLTFSLPPHQVANGIADAFIHVMEQYMTFPSDSPIQDRF"
     gene            949..1272
                     /locus_tag="PLX53_06375"
     CDS             949..1272
                     /locus_tag="PLX53_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72743.1"
                     /translation="MAMNKLTLGKYHILVVVAVLAATVVLLSCRQSKQENVKSSADKA
                     RENVVSWMESMKLEYPGYQPAEFGELTPRYNKSNITFHLYNLIEQERSKPSPSKRAID
                     SPENL"
     gene            complement(1415..1582)
                     /locus_tag="PLX53_06380"
     CDS             complement(1415..1582)
                     /locus_tag="PLX53_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72744.1"
                     /translation="MIILQGKQHCKITCMFIVKVNMQVLKAAFKKSLNAYRVESLLSG
                     LKNSSSSIIWS"
     gene            complement(1645..>2079)
                     /locus_tag="PLX53_06385"
     CDS             complement(1645..>2079)
                     /locus_tag="PLX53_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOY72745.1"
                     /translation="YPNLGAGAYIYSGEFYAGLSLPRFLQTSFNKKFDKTLKSPLYLM
                     GGYNLEISPSWVFMPSMLMGAMIGLPMSCDLTAQFLYQSKFYFGTHYRIGDALGIFFD
                     MKVNEYLSFGYAFDFSLNKLSRINTGTHELMLSYAFAPKWKF"
BASE COUNT          602 a          422 c          484 g          571 t
ORIGIN      
        1 aaaagcgatc ctgaataggc gagtcggagg ggaaagtcat gtactgctcc ataacgtgga
       61 taaaggcgtc ggcaatacca ttagctacct gatggggagg gagcgaaaag gtgagggtag
      121 ggtcgagaac ggagaatttt gggaacagca ccgggcttcc aaagctgtac ttctcgcggg
      181 tttcaagacg gcttatcacc gcacccgagt tcatttcgga acctgtagcc ggaagcgtta
      241 aaacaacccc aaatggtatg gccttggtta ctactatatt ctttattagc agatcccatg
      301 gatcggaccc ttcgtatagg ctggctgctg caataaattt tgttccgtct attactgaac
      361 ctccgccaat cgccagtatg aagtcaacat tatttttttt aaccacctct accgccttca
      421 tcagggtttc gtacttgggg ttggcttcaa ttcctccgaa ttcaaaaacg ctgaagttgg
      481 aaagtgccgc cttaatcctt tcgtaggtac cgtttttctt aatgctgcca ccgccatagg
      541 tcataagaat tctatcggtt gggttaagct gcattccgat tttttcgatt ttgtcgtttc
      601 cgaaaagaat tctgaccggg ttaaaaaatt caaaattatt catatcgtgt tgtttttatg
      661 gtattctgtc aagggggcca tgttggaaat ctactagcaa tttaaatggc tatttgaaaa
      721 taacagcatg gctgcttgct acaaaattag taaatctctg cggaaaggat tcaagccggt
      781 atggcataaa tttttaggga ttatccttgt tggacaataa agataattcc agctacgagc
      841 acgccccttc caaaccttct cgctagtcaa tactttaaaa gatttaaaaa agagtccgga
      901 atattctaag tataaattta gtagctttat caggcaaaaa ataagggggt ggctatgaat
      961 aaacttactt taggaaaata tcacatcctt gtagtcgtgg ctgttctggc agccaccgtt
     1021 gtgctccttt catgcaggca gagcaagcag gaaaatgtaa aaagttctgc tgataaggcc
     1081 agagaaaacg tggtgagctg gatggagtca atgaaattag agtatcctgg ttaccagcca
     1141 gccgaatttg gcgagctaac tcccaggtac aacaaaagca acattacttt ccacctctat
     1201 aacctcatag agcaggaaag gagcaagcca agtccaagca aaagggctat agacagcccg
     1261 gagaaccttt aagggataat cccggtgagc tgcttggcta ttcgattacc cataagtaca
     1321 acgtaaagga tatctcgggt gaaatccggc aagttgaaag tttatttctt ctcgatacgt
     1381 tactccgtgt aattacggtg cttgatgcgg atgcctacga ccagataatg gatgaggagc
     1441 tatttttcag acccgaaagt aaagactcaa ctctataagc atttaggctc tttttaaagg
     1501 cagcctttaa aacctgcata ttcaccttta cgatgaacat gcaggtaatt ttgcagtgct
     1561 gcttaccctg caaaataatc atggttgctt tggactaaac ttatagagtt ttgcatggta
     1621 atgcccaaat tcatatctcg ataatcagaa tttccacttg ggagcaaagg cgtatgaaag
     1681 cattaactca tgtgttccgg tgttgattct ggagagctta ttaagcgaga aatcgaaggc
     1741 atagccgaat gataggtatt cgttaacctt catatcgaag aaaattccca gcgcatcgcc
     1801 aatacggtag tgggtgccaa agtaaaattt gctctggtag agaaactggg cggtcaggtc
     1861 gcacgacatg ggtagtccaa tcattgcacc cattagcatt gatggcatga aaacccagct
     1921 ggggctgatt tccaggttgt agccccccat gaggtagagt ggacttttaa gggttttgtc
     1981 gaacttttta ttaaatgaag tctgaaggaa acgtggtagg gaaaggcctg cgtagaattc
     2041 gccggagtaa atgtaagcac ctgcacccag gttagggta
//
LOCUS       DAOOQX010000365         2077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_66771_length_2077_cov_3.490603, whole genome shotgun sequence.
ACCESSION   DAOOQX010000365 DAOOQX010000000
VERSION     DAOOQX010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66771_length_2077_cov_3.490603"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1095)
                     /locus_tag="PLX53_06390"
     CDS             complement(76..1095)
                     /locus_tag="PLX53_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOY72746.1"
                     /translation="MKVFPSRRIVNFFIFFLLSILLLYLAFRKVDFSHVAQGFRNVSY
                     LWIFLSLLVGLASHALRAVRWSILVEPLGKKPSFLNMFSAVMVGYIANLALPRVGEVA
                     KCGSVSRTDGIRFESLVGTVVVERAMDLVMLLLATITVVALKFDFFGKFIISRIFHPL
                     RDRALQLNGYQLAAVLVVVVAFILFVRLILRSEIFGSKISLKIRSIWNGTVDGVKSIA
                     HTNKLGQFLVLSVVMWLCYWVMTWLLLYSTPITANLTVWDGLFIMVIGSYGMVVPVQG
                     GFGAYLMITAIALGIFGITYDDGLVFAIISHESQTFLVILGGILALIYVYIIQRPDKP
                     KPQSL"
     gene            complement(1113..1463)
                     /locus_tag="PLX53_06395"
     CDS             complement(1113..1463)
                     /locus_tag="PLX53_06395"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOY72747.1"
                     /translation="MLIEVVKSKIHRVTITEANLNYIGSITIDEDLMDAANLIENEKV
                     QVVNVNNGERLDTYIIKGERGSGQVCLNGPAARKCVTGDVVIIMSYATMDFEEAKHFS
                     PTVVFPDTATNKLV"
     gene            complement(1466..>2077)
                     /locus_tag="PLX53_06400"
     CDS             complement(1466..>2077)
                     /locus_tag="PLX53_06400"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOY72748.1"
                     /translation="EKTGVDYVFMPSETEIYPQPDTRTFNFGQLDKVMEGAHRPGHFN
                     GVAQVVSRLFDIVKPNRAYFGQKDYQQLVIIRQLVRMLGLSIDIIACPIVREADGLAM
                     SSRNLLLSPECRASAPLIHRTLVEACNQIEQMNVKDMIGWVEARVNQDKNLRVEYFEI
                     SNAETLEPVSSWTDAENIIGCIAVWAGSVRLIDNIFLKHSTKG"
BASE COUNT          573 a          513 c          457 g          534 t
ORIGIN      
        1 cgccagtaaa ggtaaagtaa tgctaatagt caacctggca tcgtgttgct aaaagcttct
       61 tgccttctaa tgactttaca gggattgggg ttttggtttg tccggccttt gaattatgta
      121 aacgtatatt aaggccagga tgccgcccag tataacaaga aaggtttgtg attcgtgtga
      181 aataatggca aacaccaacc cgtcgtcgta ggtgattcca aagataccaa gggcaatggc
      241 ggtaatcata aggtatgctc caaatcctcc ctgtactgga actaccatgc catacgagcc
      301 tataaccatt atgaataaac cgtcccatac ggtaagattt gcagttatgg gggttgagta
      361 aagcagcaac catgtcatta cccagtagca aagccacatg actaccgaaa gtacaaggaa
      421 ttggcccagc ttgttggtgt gggctattga tttaactcca tcaacggtgc cgttccagat
      481 ggatcgaatt ttgaggctga ttttactgcc aaaaatttca ctccgtagaa ttagcctaac
      541 gaaaagtatg aaggcaacca caacaaccag aactgcagcc agctggtaac cgttgagctg
      601 gagcgcccga tcccttaacg ggtgaaaaat tcgtgaaatg ataaactttc caaaaaaatc
      661 aaatttcaac gctaccaccg taatcgttgc tagcagcagc attaccaggt ccattgcgcg
      721 ttccaccacc accgttccaa ccaacgattc aaagcgaata ccatcggttc tgctaacact
      781 tccgcatttt gctacttccc ctacccgtgg gagtgctagg tttgcaatat accccaccat
      841 cacggcgcta aacatgttca ggaatgaagg ttttttccca agaggttcaa caagaatgct
      901 ccaacggaca gcccgtagcg catgggatgc caatccaacc agtagcgaaa ggaaaatcca
      961 taagtagctt acgtttctaa atccctgtgc aacatgggaa aagtcaacct tgcggaaggc
     1021 aaggtagagc agcaatatgg agagtagaaa gaagatgaaa aagttaacga ttcgacgtga
     1081 agggaaaact ttcaaggcga acgggttaag tgttaaacaa gcttattagt tgcagtatcg
     1141 ggaaacacaa cggtaggaga aaagtgcttg gcttcctcaa aatccatggt ggcgtacgac
     1201 ataatgataa ccacatcgcc ggtaacgcac ttgcgggccg cagggccgtt caggcacacc
     1261 tgccccgatc ccctttcgcc tttaataata tacgtgtcga gccgctcccc gttgttcacg
     1321 tttaccacct gtaccttttc gttttcgatc aggtttgcag catccattaa gtcctcatca
     1381 atggttatgc tcccaatata gttcaggttt gcctcggtta tggttacccg gtgaattttc
     1441 gattttacta cttctatcaa catagctacc cctttgttga gtgtttgaga aaaatgttat
     1501 ctatcagcct aacgctaccc gcccaaacag caatacatcc aataatgttc tcagcgtcag
     1561 tccagctgct tacgggctcc agtgtttcgg catttgaaat ctcaaagtac tccaccctaa
     1621 ggtttttatc ctggtttacc cgtgcttcta cccacccaat catatcttta acgttcattt
     1681 gctcaatttg gttacaggcc tcaacaaggg ttctgtgaat taacggtgcg ctagccctgc
     1741 attctggtga cagaagaagg ttacgactgc tcatggccaa gccgtcggct tcacggacta
     1801 tggggcatgc aataatgtca atgctgagtc ccagcattct aaccagctgc ctgatgatga
     1861 ccagctgctg gtaatccttt tgcccaaagt aagcccggtt tggcttcaca atatcgaaaa
     1921 gcctgctaac cacctgtgca actccattga agtggccagg cctgtgagcg ccctccatca
     1981 ccttatcgag ttgtccgaag ttaaacgtgc gggtatcggg ttgggggtag atttcagtct
     2041 cggagggcat aaacacgtaa tcaacccccg ttttctc
//
LOCUS       DAOOQX010000366         2071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_67040_length_2071_cov_3.396825, whole genome shotgun sequence.
ACCESSION   DAOOQX010000366 DAOOQX010000000
VERSION     DAOOQX010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67040_length_2071_cov_3.396825"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLX53_06405"
     CDS             complement(<1..405)
                     /locus_tag="PLX53_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72749.1"
                     /translation="MFRRFCLLVVSFLFIYVKSGFPQPQETEPSITSRLQRDTILIGD
                     QITFSIEVKSIKPLRVVFPNFTDSLGNGVEVVGIPTADSLTKANYHHYSMNMLITAFD
                     SGTLVVPSIPVVLASGHTTDTLFTQPHALHVNL"
     gene            complement(405..1292)
                     /locus_tag="PLX53_06410"
     CDS             complement(405..1292)
                     /locus_tag="PLX53_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOY72750.1"
                     /translation="MEASEILKRVRKIEIKTRGLSQHIFAGQYHSAFKGKGMAFSEVR
                     EYRYGDDIRSIDWNVTARYNQPYVKVFEEERELTVMLLVDVSGSQQFGTDFLLKRQAL
                     TEVAAVIAFSAILNNDKIGAILFSDKVEKFIPPQKGRRHILRIISELLNFKPEGTGTN
                     ITEALKFLTNGIKKRCTAFLLSDFLDVERLSLKPLFADALTIAGNKHDLIGIRIFDKR
                     ESDFPSVGLVKVRDNETGKYIWVDSNAPAVRSNFSNRWSQLSDNLKSTFTKASVDWVS
                     IRTDEDYVKPLISLFKHRS"
     gene            complement(1298..1717)
                     /locus_tag="PLX53_06415"
     CDS             complement(1298..1717)
                     /locus_tag="PLX53_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72751.1"
                     /translation="MSIFRILPLALLLVNLSIPMQGQNLIGLTKGEILKLVPTSCKGF
                     NFVKDIQNGDKSFIKFENPTDEQTLLFMLNSKEICTSVSRMYNTWLYDKVYKDLCCEY
                     KQVSMDTWIDSKNGNDYEVKLKRGEWFITVTIRPFIK"
     gene            complement(1719..>2071)
                     /locus_tag="PLX53_06420"
     CDS             complement(1719..>2071)
                     /locus_tag="PLX53_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOY72752.1"
                     /translation="DIINARKVVHEVYMDEKIEKYILDIVFATRFPADVNLQKFAPMI
                     SYGASPRATISLAIASKAYAFIKRRGYVIPEDVRAVCHDVLRHRIGLTYEAEAESITS
                     EDIITEILNSVEVP"
BASE COUNT          595 a          450 c          432 g          594 t
ORIGIN      
        1 caggtttaca tgtagcgcat gaggctgggt aaaaagtgtg tcggttgtgt ggcccgatgc
       61 caacactacc gggattgagg ggacaacgag agtgccacta tcaaatgcag ttataagcat
      121 gttcatgcta tagtggtggt aattagcttt ggtaagcgag tcggctgttg ggatgccaac
      181 cacctcaacc ccgttgccaa gggaatcggt gaagttcggg aagacaaccc gtaggggctt
      241 tatgctttta acctcaatgc taaaggtaat ttgatcgcca attaaaattg tatccctttg
      301 aagccgcgag gtgatggatg gttcagtctc ctgcggttga gggaaaccac tttttacata
      361 aatgaaaaga aaagaaacaa ccagtaaaca aaatctccta aacattagct tctatgttta
      421 aaaagggaaa ttaaaggttt tacgtaatcc tcatcagttc ttatagaaac ccaatcaaca
      481 ctagccttag taaatgttga tttaagattg tcggaaagtt gactccatcg gttgctaaaa
      541 ttgcttctta cagctggagc gtttgagtca acccagatgt acttgccggt ttcgttgtcc
      601 ctcactttga ctaacccaac tgaagggaaa tcgctttcgc gcttatcgaa tatgcgaatg
      661 ccaattaaat cgtgtttgtt acctgcgatg gtaagagcgt cggcaaacaa aggttttagg
      721 gaaagccttt caacatcaag aaagtcgctc agtagaaagg cggtacaccg ttttttgatc
      781 ccgttggtaa ggaatttaag ggcttcggta atattcgtgc cggtgccctc gggtttaaag
      841 ttaagcagct cgctgattat tcgcaggata tgccttctgc ccttttgggg tggaataaac
      901 ttttcaactt tatcgctaaa aagaatagcg ccaattttat cattattaag aattgcagaa
      961 aaagcaataa cggcagctac ttcagtcaaa gcttgacgtt taagtaaaaa atctgttcca
     1021 aattgctgcg agccgctaac atcaaccagc aacataacag taagctcccg ctcctcttca
     1081 aaaactttta catagggttg attatagcgc gccgtaacgt tccagtcaat gcttcgaata
     1141 tcatccccat accggtattc cctaacttcg ctgaaagcca tacccttacc cttaaatgcg
     1201 ctatgatatt gacctgcaaa gatatgctgg gatagaccgc gagtcttaat ctcaattttt
     1261 cgaacccgtt tcagtatttc agatgcttcc atacagccta ttttatgaac ggtcgaatag
     1321 taactgtaat aaaccactca ccccgcttga gcttcacctc gtaatcgttt ccgtttttac
     1381 tgtcaatcca ggtatccatg cttacctgtt tatactcaca acacaaatct ttataaacct
     1441 tgtcgtagag ccatgtattg tacattcttg agaccgatgt gcatatttcc ttgctgttga
     1501 gcatgaaaag gagagtttgc tcatcggtgg gattctcgaa ctttataaaa cttttatctc
     1561 cattctggat atccttaaca aaattaaatc ccttacagct ggtaggtaca agcttcagaa
     1621 tttcaccctt ggttaagcct ataaggtttt gcccctgcat gggaatgctg agattcacaa
     1681 gcagtagagc cagaggcaaa attctgaata tgctcattct agggtacttc aaccgaatta
     1741 agaatttcgg taataatatc ctcagaagtg atgctttcgg cctccgcttc gtaggtaagc
     1801 ccaattctat ggcgcagaac atcgtgacac acagctctta cgtcttcggg aataacgtaa
     1861 ccccgacgtt taataaaagc atatgcctta gaagctatag ccaggctaat agttgcacgt
     1921 ggtgaggctc cgtagctaat cataggagca aacttctgca ggttaacatc ggcagggaat
     1981 cgagtggcga aaacaatgtc gagaatgtac ttctcaattt tttcatccat gtaaacctcg
     2041 tgcacaacct ttctggcatt gataatatca g
//
LOCUS       DAOOQX010000367         2067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_67259_length_2067_cov_2.593936, whole genome shotgun sequence.
ACCESSION   DAOOQX010000367 DAOOQX010000000
VERSION     DAOOQX010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67259_length_2067_cov_2.593936"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PLX53_06425"
     CDS             complement(<1..853)
                     /locus_tag="PLX53_06425"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOY72753.1"
                     /translation="MSMEQYLGVYFLGIGGIGMSALARYFALKGRVVAGYDRTPSAIT
                     SQLAEMGIGIHFTDDAELIPSEFRNPATTLVVYTPAVPETLAELAYFRRGGFQLLKRA
                     QVLGMLSLGYQTLAVAGTHGKTTTSTLLAYLLGSSSVGCDAFLGGISKNYGSNLVTAD
                     KGRKLLVAEADEYDRSFLNLHPQLAVITSVDPDHLDIYGDYERVVEAFNQFAGQVKPN
                     GTLIAKSGLGIAPIPERGVTRLTYGLSSDADFYPVNLSLNEGCYGFSLETPNGTIDDL
                     KLGVPSLR"
     gene            complement(935..2062)
                     /gene="murG"
                     /locus_tag="PLX53_06430"
     CDS             complement(935..2062)
                     /gene="murG"
                     /locus_tag="PLX53_06430"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOY72754.1"
                     /translation="MEKNQLKVIISGGGTGGHIFPAIAIANAVKELSPEAEILFVGAL
                     GRMEMERVPQAGYRIVGLPIIGIRRSLSPQNIMLVFKLIRSLRLSRKIIADFRPDVVV
                     GVGGYASGPILRAAQKRGIPTLIQEQNSFAGITNKMLAAGAHKICVAYDGMERYFPKE
                     KIIKTGNPVRQDIETLKVTREEAFRFFNLSPEQKVLLVLGGSLGAGTINQSLRHKLDA
                     IARHPEVAVIWQTGKFYYEAINQELAGYSGRNIRLFDFINRMDMAFAAADLVISRAGA
                     GTISELCLVGKPAILVPSPNVAEDHQTHNAEALVKRNAAVMVADSDAEPTLVETAFSL
                     ISNEEKLRELAQNILTMALPNSATLIAGEVLSLANANSKSL"
BASE COUNT          522 a          554 c          478 g          513 t
ORIGIN      
        1 acctcaagct tggaactcca agcttgaggt cgtctatcgt tccattaggc gtttcgagcg
       61 aaaagccgta acaaccctcg ttaaggctaa ggttgaccgg gtagaaatcg gcatctgacg
      121 acagcccata ggttaaccgg gtaacgcccc tttcagggat aggcgcaatt cccagaccgg
      181 atttagctat caacgttccg ttaggtttaa cctgaccggc aaactggttg aacgcctcca
      241 ctaccctttc ataatcaccg taaatgtcga gatgatcggg gtcaaccgat gtaataacgg
      301 ccagctgggg gtgcaggttc aaaaaactgc gatcgtactc gtcggcctcg gccacaagca
      361 gctttctacc cttatcggcg gtaaccaggt tgctgccgta attttttgag attccaccca
      421 aaaaagcatc gcaaccaacc gatgagcttc ccagcaggta ggcaagcagg gttgaggtgg
      481 tagttttgcc gtgggttccg gcaacggcca atgtttgata ccctaatgac agcattccaa
      541 gcacctgggc acgttttaaa agctgaaatc cacccctgcg aaagtaggct agctccgcta
      601 aggtttcggg aacagcagga gtgtaaacaa ccaacgtggt tgcaggattt ctaaattcag
      661 agggtatcag ctctgcatcg tcggtaaaat gaatgccaat acccatctcg gccagttgcg
      721 atgtgatggc ggaaggggta cggtcgtacc cggccacaac ccgaccctta agggcaaaat
      781 atcgtgccag cgcactcatt ccgataccgc ctatacccaa aaaataaacc cccaaatact
      841 gctccatgct catatctagc gccttacacc ttaagttttg ccctaatcaa tttatttaac
      901 acgctgaaac actgtattta aacatcgatt tcacctacaa agatttactg ttagcattag
      961 ccagcgatag cacctcacca gcaatcagtg ttgccgagtt tggcaacgcc attgttaaaa
     1021 tattctgtgc cagttcacgc aatttctcct cgttggagat aagcgaaaag gccgtttcga
     1081 caagtgtagg ttctgcatcg ctatcggcaa ccattacggc agcattccgt ttaaccagag
     1141 cttcagcgtt gtgggtttga tggtcttcgg ccacattagg cgatggaacc agaattgccg
     1201 gtttgcccac aaggcagagt tccgatatgg ttccggctcc tgcccttgaa ataaccagat
     1261 cagctgctgc aaaggccata tccatccggt ttataaaatc gaaaagcctg atattcctac
     1321 cggagtaacc tgccaactcc tggttgatgg cttcgtagta aaacttcccg gtttgccata
     1381 ttaccgccac ctcggggtga cgggcaattg catcaagctt atgccgcagg ctctgattaa
     1441 tggttccggc accgaggctt cctcccaaca caaggagaac tttctgctcg ggagagaggt
     1501 tgaaaaatct aaacgcctct tcccttgtta ccttaagagt ttcaatgtcc tgccgcaccg
     1561 gattgcctgt tttaataatc ttttccttcg ggaagtagcg ttccatgccg tcgtatgcca
     1621 cgcaaatttt gtgagcacca gctgccagca tcttgttggt aatgccggcg aaggaattct
     1681 gctcctgtat cagcgttggt atccctcgtt tttgagctgc cctgagaatg ggcccgctgg
     1741 catagccgcc tacaccaacc accacatcgg gtctaaagtc ggctataatt ttacgagaca
     1801 agcgaagact tctaatcagc ttaaaaacca gcataatatt ctggggtgaa aggcttcgtc
     1861 taatgccgat tatgggcagg ccaacaatac ggtatcctgc ctgcggaact ctttccatct
     1921 ccatacggcc caacgctcct acgaagagaa tttcggcctc gggcgacagc tctttcacgg
     1981 cgttggcaat ggcaatagcc gggaaaatat ggccgccggt tcccccgccg ctaattatta
     2041 ctttcagctg gttcttctcc atcaaaa
//
LOCUS       DAOOQX010000368         2064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_67396_length_2064_cov_3.577899, whole genome shotgun sequence.
ACCESSION   DAOOQX010000368 DAOOQX010000000
VERSION     DAOOQX010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67396_length_2064_cov_3.577899"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLX53_06435"
     CDS             complement(<1..334)
                     /locus_tag="PLX53_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY72755.1"
                     /translation="MSTFLFDSIVFGPVRSRRLGASLGMNLLPPDRKVCSFNCVYCEC
                     GWTDSIDSDMLPSRGQVQAALEYKLKKMISGGEGLDVITFAGNGEPTLHPDFSGIIDD
                     TIELRNTYF"
     gene            complement(626..1246)
                     /locus_tag="PLX53_06440"
     CDS             complement(626..1246)
                     /locus_tag="PLX53_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72756.1"
                     /translation="MKIIRSFFVLIALTTIAGVVNAQMFVSGSINLSTSMPKTTTGSV
                     TTEGARYTYFRVAPAVGYFLNDKLAVGAGVSYSLQKQKQNDVANLTSMFGITPFVRYY
                     AVSLGDVNIFAQGNVGIEFGANKTKNDGNTTDGPKYLNFGISVMPAISYSLGEKLAIE
                     AFLGSVYYSFNREKDEIAGETHTLKTNGFGLNFDMSSISLGVIYKF"
     gene            1672..>2064
                     /locus_tag="PLX53_06445"
     CDS             1672..>2064
                     /locus_tag="PLX53_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOY72757.1"
                     /translation="MAVERKIYPVEGMSCASCAMTVEKTIKNQPGVEDAGVNFANSTV
                     WVTFDPKAVKPEDLRDSVRSAGYDLIVDAVDVVKVGEEAKQKYADELKLRFWMSAAFS
                     VPLLVLGMFFMDWKLTPWLSAIMAAPVVF"
BASE COUNT          593 a          458 c          438 g          575 t
ORIGIN      
        1 ggaagtatgt attcctcaac tcaatagtat cgtctataat tccggaaaaa tcaggatgaa
       61 gtgtaggctc accattgcca gcaaaggtaa ttacatcaag gccttcaccc ccgctaatca
      121 ttttctttag cttatactca agggctgcct gaacctgccc ccgtgaaggg agcatatccg
      181 aatcaatgga gtcggtccag ccacactcgc agtacacgca gttgaacgag caaacttttc
      241 ggtcgggcgg aagcaggttc attcccagcg aggcaccaag cctccggcta cgcaccgggc
      301 caaacacaat gctatcgaac agaaaggttg acatagctgc tgatttttcg caaagatacc
      361 cttttggagc aagatggtgt gcccgtgttc aacttttgga aattatgaac aattcaaagc
      421 ttgacattct attaaatacc aatgtgttgg gtatgctgca tacacgatac taaagcacga
      481 accagctgat tcatcaactt ctgcataaca atctataaaa attatcggcg gcaaccccca
      541 actttaaatc gtttggggtt gccgccagat gaataaaacc tccagtggga ttaactccta
      601 aaggtggaaa tccccttaat gcttattaaa acttgtaaat aacgcctagc gatatgctag
      661 acatgtcgaa attcaagcca aaaccgttag tttttaaagt gtgagtttct ccggcaatct
      721 catccttttc cctgttaaaa gaatagtaca cactacccag gaaggcttca atagccagct
      781 tttcgcccaa gctgtaggag atggcaggca taacgctaat accaaaattt aggtatttag
      841 gcccatcagt agtattccca tcattcttcg ttttgttggc gccaaactct attccgacat
      901 taccttgtgc aaaaatattc acatcaccca gacttacagc gtagtacctg acaaacggag
      961 taattccaaa catgctggta agattggcca catcgttttg cttttgcttt tgaaggctat
     1021 agctaacacc tgcacctact gccaacttgt cgtttaaaaa atacccgact gcaggggcaa
     1081 cacgaaaata ggtgtatcgt gctccctcgg ttgtaaccga cccggttgta gtttttggca
     1141 ttgaggttga aaggttgatg ctaccgctaa caaacatttg cgcatttact acgcctgcga
     1201 tcgtggttaa tgcaattaat acaaaaaaac tcctgatgat tttcattacg attcatattt
     1261 aatgttaggc gtactcttag gtttttcggc atacacctat aattaaactc agcaattgac
     1321 gtattgttca atttcgatat tatgacggtt atagggttta agccatccct accatattat
     1381 cgccgatagc tacttttgcc tagaaaaatt atttcaactt aagtaaattt gcaacagcaa
     1441 aaattgcgcc aactgccatt aggctagagg ctgaatcccc ctttacgtat caagtttgct
     1501 ctgctgattc ctatgcccca gagtgttatg cgaattgctt cccacaagta gtatgcattt
     1561 ggtaagccat tagctggttt gatggaaaga aaatacacgg tttaagaata agtttttata
     1621 tcatgcaaaa cgttaaacac aaatggattt gacctgttta gtaagcaaaa aatggctgtg
     1681 gaaagaaaaa tatacccggt agaaggaatg tcgtgcgcca gctgcgccat gaccgtggaa
     1741 aaaaccataa agaaccaacc gggggtggag gatgcgggcg ttaactttgc caacagcacg
     1801 gtttgggtta cttttgatcc taaggctgtt aaacctgaag atttaaggga ttctgttcgt
     1861 tcggctggct acgatctcat agttgatgcc gttgacgtgg taaaagttgg cgaggaagcc
     1921 aagcagaaat acgccgatga gctaaaactg cgcttctgga tgtcagcagc attctcagtg
     1981 cctcttctcg tgttgggaat gttctttatg gattggaagc taaccccgtg gctgtcggcc
     2041 attatggctg ctccggtggt attt
//
LOCUS       DAOOQX010000369         2060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_67612_length_2060_cov_2.373067, whole genome shotgun sequence.
ACCESSION   DAOOQX010000369 DAOOQX010000000
VERSION     DAOOQX010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67612_length_2060_cov_2.373067"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PLX53_06450"
     CDS             <1..610
                     /locus_tag="PLX53_06450"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOY72758.1"
                     /translation="ALLGWNPGTERELFNMEELIQIFSLERVNKSGARFDPEKAKWFN
                     HQYLIRKGNDEVANLFMPILEQYGVQVQMDKLVRIVGLVKERVNFIHELWGQTSFFFE
                     RPLNYNSQTVAKFWNNDSSVMMNILVEKLSGISTWTALNIEQSVKELIGEKGYGMGKV
                     MNTIRLALVGESKGPGVADICEILGKEETLQRIRQAVLILGK"
     gene            complement(681..>2060)
                     /locus_tag="PLX53_06455"
     CDS             complement(681..>2060)
                     /locus_tag="PLX53_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOY72759.1"
                     /translation="SYSTIALASIILLLAACSGTKKSVDTIPSPSWVQNRPTSSIYYV
                     GIGSAPKIGSPNDYQQAAKQNALADMASEISVKISSNSVISAFETQQNFYEDYSASIR
                     AEAQKELEGYEMAEAWEDSQNYWVYYRLSKETYRKISDEKKAKAASLSLDYYDKALKS
                     AENGDYRTCLLQVVRALETLKPYFAESVTASYQGREIFLGNELIQLLTETLNNLLLNG
                     PSEVLARMGQPIPAGSLTYSVTNRIGIPQKGMPLRASYSEKPMLMNKSITGSDGKACF
                     SVDRVSSSRNVEKIAVVLDFSAIVQEATTDFDLRKLLSRFQVPSVETSIRIDKPTFSV
                     VTREMLFDKPAQKNVLAEAIKRKVTDEGFPIAEKTSDADFAITIEASTAKTGESGSYK
                     QASLSVLIYVTNAQGVEVYRRSLDPITGSHFDWEAAGLSTFDKAAKRLESSTVPEIIQ
                     VVVLGKPSY"
BASE COUNT          518 a          448 c          514 g          580 t
ORIGIN      
        1 tgccctgctt ggatggaatc cgggaacaga aagggagctc ttcaatatgg aggagctaat
       61 tcaaattttc agcctcgagc gggtaaataa gtccggtgcc cgattcgatc ccgagaaggc
      121 aaagtggttc aaccaccagt acttaatccg caagggcaac gatgaggttg ccaacctttt
      181 tatgcctatt ttggagcagt atggcgttca ggttcaaatg gacaaacttg tccgaattgt
      241 tgggctggtc aaggagcgag tcaattttat tcatgagctt tggggccaaa ccagcttctt
      301 ttttgaaagg ccactgaact acaatagcca aacggttgcc aagttttgga acaatgattc
      361 ttctgtgatg atgaatatcc tggtagaaaa gctatccgga atttcaacat ggacagcgct
      421 caacattgag caatcggtaa aggagctgat tggagaaaag ggctatggta tgggtaaagt
      481 tatgaatacc attcggcttg cgcttgttgg cgaatcaaag ggccctggcg ttgccgatat
      541 ttgcgagatt cttggcaaag aggaaaccct acagcgaata aggcaggccg ttttaattct
      601 gggtaagtag agctttaggg ggttaagcca aagggtaaat tctctcatta ctgtgtttac
      661 tctggtaaac gttgcgccaa ctagtaggat ggcttgccca aaacaactac ctgaataatt
      721 tccggcactg ttgagctctc aagccgtttg gccgctttgt cgaaggtgct taagccagca
      781 gcctcccagt caaaatgaga accggttatg gggtctaaac ttcggcggta aacctcaaca
      841 ccctgtgcgt tggtaacgta tatcaaaact gaaaggcttg cctgcttgta actgcccgat
      901 tctccggttt tagcagttga ggcctcaatg gtaatggcaa aatctgcatc ggatgttttt
      961 tcggctatcg ggaatccctc atcggtgact ttacgcttta tggcttcggc cagaacgttc
     1021 ttttgagcgg gcttgtcgaa cagcatttcc ctggttacta ccgaaaatgt aggcttatca
     1081 attcttattg aggtttcaac cgaaggaacc tgaaatcggg atagaagctt acgcaagtca
     1141 aagtcggtgg tggcctcctg aactattgcg ctaaagtcca gcacaacggc tattttttcg
     1201 acattgcgtg aggagctgac acgatcgacc gaaaaacaag ctttgccatc actaccggta
     1261 atactcttgt tcatcagcat tggcttttcg gagtagcttg ccctaagagg cattcccttt
     1321 tggggaatac caatacgatt ggtaacgctg taggttaacg agccggcagg aattggttgt
     1381 cccattcgtg ccaaaacctc ggaaggtccg ttaagcagca ggttattcag ggtttcggta
     1441 agcagctgga ttagctcatt tcccagaaag atctcccttc cctggtagct ggcagtaact
     1501 gattcggcga agtatggttt gagagtctcc agtgccctaa ctacctggag caggcaggtt
     1561 ctgtaatcac cattttcagc acttttcaat gctttatcat agtagtcgag cgagagcgaa
     1621 gctgcttttg cctttttctc atctgaaatt ttccggtagg tttctttcga aaggcggtag
     1681 taaacccagt agttttgtga atcctcccat gcttcggcca tctcatatcc ttcaagttcc
     1741 ttttgagctt cggcacgtat gcttgccgag taatcctcgt aaaaattttg ctgggtttca
     1801 aaggcgctta tcacggagtt agacgatatc tttaccgaaa tctcgcttgc catatcagcc
     1861 agtgcatttt gtttggcagc ttgttggtaa tcgtttgggc tgcctatttt aggggcagaa
     1921 cctataccaa cgtagtaaat tgaggaggtt gggcgatttt gtacccacga tggggacgga
     1981 attgtatcaa cagatttttt tgttccgcta catgcagcca gcaacaatat gatggaggca
     2041 agagcaatcg tcgagtagct
//
LOCUS       DAOOQX010000370         2058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_67701_length_2058_cov_3.169246, whole genome shotgun sequence.
ACCESSION   DAOOQX010000370 DAOOQX010000000
VERSION     DAOOQX010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67701_length_2058_cov_3.169246"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PLX53_06460"
     CDS             complement(<1..899)
                     /locus_tag="PLX53_06460"
                     /inference="COORDINATES: protein motif:HMM:NF015592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HOY72760.1"
                     /translation="MNTYLKHDEWRIIEDNFIPENTRASESIFSIGNGAMGQRANFEE
                     KYSGNSLQGSYIGGVYYPDKTRVGWWKNGYPEYFAKVINSTNWIGISVKVNGEELDLA
                     TAKVLNFCRILNMKEGYLERSFTCELPNGIKLEVKSTRFLSMVDTEIGAIRYSIKPVN
                     SNASIQISSYLDADVHNEDSNYGEKFWDVIDVRGGEIFSYIISQTKKLDFQVGHAMRN
                     LLFINGQPQNLKPQVNSGQMYVDNRFTVNASSGDEVILYKYAAVISNLNHEKGKFTKV
                     LETLIEGASEKGFDKLFDEHRKA"
     gene            complement(916..1578)
                     /gene="pgmB"
                     /locus_tag="PLX53_06465"
     CDS             complement(916..1578)
                     /gene="pgmB"
                     /locus_tag="PLX53_06465"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HOY72761.1"
                     /translation="MSSIKGCIFDLDGVVVDTAKYHYQAWKRLANELGFEFDESHNER
                     LKGVSRMRSLEILLEVGGLSFPQEKMEQLAARKNEWYVEYISRMTPNEILPGSKELLE
                     ELRAKGIKLALGSASKNTPLIMDRIGLKDHFDAIIDGNIISIAKPAPDIFLAASKAIG
                     VEPPCCIVFEDAEAGVEAAINAGMRVVGIGNPQVLKRANLVVPNLKNFTFQYLVNSLS
                     EK"
     gene            complement(1571..>2058)
                     /locus_tag="PLX53_06470"
     CDS             complement(1571..>2058)
                     /locus_tag="PLX53_06470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY72762.1"
                     /translation="FAYNEAANWVGVLFGLYSVFAALFAIFLNKIADKLGRIKTYSIS
                     LLLGGLGFLSIYLFNNQYALIISMLGIGIAWAAILAMPYAILANSLPAERMGVYMGIF
                     NITVVVPQIVSGIVLGTMLKSFFNDEAIYMILLAGFSMILASASAMLFVKDNSKVLPN
                     E"
BASE COUNT          581 a          475 c          408 g          594 t
ORIGIN      
        1 caagcttttc ggtgctcatc gaacagcttg tcgaaaccct tttcgcttgc cccctcaata
       61 agtgtttcca gaactttggt aaactttccc ttttcatggt tcaggttgga tataacagcc
      121 gcatacttgt agaggatcac ctcatcgccg gagctagcat taaccgtaaa cctgttgtca
      181 acatacatct gcccggagtt gacctgtggt tttaggtttt gaggttgccc attaataaaa
      241 agcaagtttc gcatggcatg tcctacctga aaatcgagtt ttttagtttg gctgattatg
      301 tagctaaata tttcaccgcc ccttacatca ataacatccc agaatttctc gccgtagttt
      361 gagtcttcgt tatgcacatc tgcatcgagg taggagctaa tttgaatgga agcattggag
      421 ttgacgggtt taatgctgta acgaatagca cctatttcgg tatcgaccat gctcaaaaaa
      481 cgggttgact tcacctccag cttgatgccg ttgggtagct cacaagtgaa tgagcgttcg
      541 aggtagcctt ccttcatgtt aagaatcctg caaaaattaa gcactttagc agtagccaaa
      601 tccagctcct caccatttac ctttaccgaa atgccaatcc aattagtgga gttgattacc
      661 ttggcaaagt attcagggta gccatttttc caccagccca ctcgggtttt gtcggggtag
      721 taaactcctc caatatagct tccctgaagg gagtttccgc tgtacttttc ctcgaagtta
      781 gccctttggc ccattgcacc attccctatg ctaaaaatac tttctgaagc acgggtattt
      841 tcaggaataa aattatcctc gatgatgcgc cattcatcat gtttaaggta tgtattcata
      901 agagtatgta tgttactatt tttctgataa tgaattgact aaatattgaa atgtaaaatt
      961 tttcaggttg ggtacaacca aatttgccct ttttagtacc tgagggtttc cgatacccac
     1021 cacgcgcatg cctgcattta ttgctgcttc aactccggct tcggcatctt caaatacaat
     1081 acaacatgga ggctctacac caattgcttt tgacgctgcc aaaaaaatat caggagcagg
     1141 tttagcaatc gaaatgatat tgccgtctat aatagcatca aagtgatctt ttagtccaat
     1201 tcggtccatg attaggggtg tatttttgct ggcagaccca agcgccagct taattccctt
     1261 agctctaagt tcttccagaa gttctttgga accgggaaga atttcattcg gggtcatccg
     1321 actaatgtac tccacatacc actcattctt tcgggcggca agttgttcca tcttttcctg
     1381 gggaaaggaa aggccgccaa cctcaagaag gatttcgagt gatcgcatcc tgctgacccc
     1441 ctttaacctt tcgttgtggc tttcatcaaa ttcaaaccct agctcattgg ccaacctctt
     1501 ccatgcctgg taatggtact tagctgtgtc aaccacaacg ccatccaaat caaagatgca
     1561 tcccttaatg ctactcattt ggtaaaactt tactattatc cttaacaaag agcattgctg
     1621 aagcagaagc gagtatcatg gaaaatccgg caagtagaat catgtagatg gcttcatcgt
     1681 tgaaaaaact cttcaacata gttcccagca cgataccact aacaatttgc ggtacaacaa
     1741 cagtaatgtt gaaaattccc atgtaaacgc ccatcctttc agcgggcaga gagttggcca
     1801 gaattgcata gggcattgca agaattgctg cccatgcaat acctattccc aacatggaaa
     1861 taataagagc atactgatta ttgaaaaggt agatggaaag aaaacccaag ccgccaagca
     1921 gcagagaaat agaataagtt ttaattcttc caagcttgtc ggcaatctta ttaagaaaaa
     1981 tggcaaatag agcggcaaat acgctgtaca gtccaaaaag aaccccaacc cagttggctg
     2041 cttcgttgta ggcaaagg
//
LOCUS       DAOOQX010000371         2056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_67798_length_2056_cov_3.241379, whole genome shotgun sequence.
ACCESSION   DAOOQX010000371 DAOOQX010000000
VERSION     DAOOQX010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67798_length_2056_cov_3.241379"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..800)
                     /locus_tag="PLX53_06475"
     CDS             complement(75..800)
                     /locus_tag="PLX53_06475"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOY72763.1"
                     /translation="MDAVNIALATTDKDKSIGKVSKFNNILAGLLIVILSIILFFAIL
                     IYAIPSLTELVMKVNGFAPPSSEIFSPTPKDYAKIKLLTGKMQQKYVALTPPRPYIVV
                     NTTYNRFKLYKNRRLIREGFCSTGSYTLLKSHFNREWIFQTPKGFYRIQGKITNPVWR
                     RPDWSFVEEGLPIPSQNDPSRWEAGVLGDYAMSIGDGYLIHGTIYKRFLGMPVTHGCI
                     RMNDDDLEAVYKTLELGSKVYIY"
     gene            complement(807..1952)
                     /locus_tag="PLX53_06480"
     CDS             complement(807..1952)
                     /locus_tag="PLX53_06480"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOY72764.1"
                     /translation="MSRKSKRIAVIILASLVVATGISVLFYKLIPEPPIGDVDYARLC
                     LQQASKSKAQSYSKKSFKQAKADYDSAMKCWQKENEKFILFRDYSMVAKLAQKSAKEA
                     RNAMNNSQNLRKNMVVAVKDKIELLNGLILNVNELFNRVPLPQEVRDKISRGKLLLKE
                     AQLYFERGEYLSAQDKIVLAEDMLMSSYNRTLDDLEEYFNSQPYWRKLVNSTIENSAK
                     TNGNAIIIDKFARKLYIYQSGVKKLEFEAEFGKNWMGRKRLKGDKATPEGIYLVKTKI
                     PQNKTKYYRALLLNYPNNDDVEMFRREKQQGKLPANAQIGGLIEIHGNGGKGTDWTDG
                     CIALSDNDMLKVYNFIQVGTPVVIVGSLADFKSITENFKLRKHTERS"
BASE COUNT          575 a          432 c          407 g          642 t
ORIGIN      
        1 cagggccaaa gcgaagatgg gaaggcaagc cagaagaaaa attttaagga attttttttg
       61 cattagatca gctattagta gatataaacc tttgagccta gttcgagggt tttatatacg
      121 gcttccaggt catcatcgtt cattcggatg cacccatgcg taacaggcat tccaagaaaa
      181 cgcttataga tagttccgtg aatgaggtag ccatcaccaa tgctcatggc gtaatcgcct
      241 aaaactcctg cttcccatcg ggaaggatcg ttctgggaag gaattggaag cccctcttcc
      301 acaaacgacc agtccggcct ccgccaaaca ggattcgtta tctttccttg aattcggtaa
      361 aaccccttgg gggtttgaaa aatccattcc cgattaaagt gcgatttgag gagtgtatag
      421 cttccagtgc tgcagaatcc ctcacgaatt aacctcctat tcttatatag tttaaaccta
      481 ttataggtgg tattaacaac aatgtatggc cttgggggtg ttaaagcaac atatttttgc
      541 tgcattttgc cggtcaaaag tttgattttg gcataatcct taggtgtggg tgaaaaaatc
      601 tcagagctgg ggggagcaaa accattaacc ttcattacca gctcggttaa tgagggaata
      661 gcgtaaataa gtatggcaaa aaaaaggata attgaaagaa tcacaattaa cagtccggct
      721 aggatattgt taaattttga tacttttcca atcgatttgt ctttatcggt agtggcaagg
      781 gctatattta cggcatccat atataattag ctcctttctg tatgttttct caacttgaaa
      841 ttttcggtaa tgcttttaaa atcggctaaa gagcctacaa taacaaccgg tgttccaacc
      901 tgaataaagt tgtaaacctt aagcatatca ttatcactaa gagcaatgca tccgtcagtc
      961 cagtctgttc ccttaccgcc gttaccatga atttcaatta agccgccgat ttgagcattt
     1021 gcaggaagtt taccttgctg cttttccctt ctaaacattt caacatcgtc attgttcggg
     1081 tagtttaaga gaagagccct gtagtacttc gttttattct gggggatttt ggtttttaca
     1141 aggtaaatac cctcaggggt tgccttatcg cctttaagcc tttttctacc catccagttt
     1201 ttaccaaact cagcttcaaa ctcaagtttt ttcacacccg actggtagat ataaagtttt
     1261 cgggcaaact tatcaataat aatggcgttt ccgttggttt ttgcagagtt ctcaatggtg
     1321 ctatttacca gcttacgcca gtatggttgg gagttaaagt actcttccaa gtcgtcaagt
     1381 gttctattgt aggatgacat gagcatatct tctgcaagaa caattttgtc ctgggctgac
     1441 aggtactcac ccctttcaaa atatagctgg gcttccttaa gcagcagctt cccccgtgaa
     1501 atcttatccc ttacctcctg gggaagtggc accctgttga aaagctcatt tacatttagt
     1561 atcaggccat tcaggagttc aattttatcc ttaacagcta caaccatgtt cttccttaag
     1621 ttttgggaat tgttcatggc atttcttgcc tctttcgccg atttttgggc aagttttgca
     1681 accatggaat agtcacggaa caggataaac ttttcatttt ccttttgcca gcacttcatg
     1741 gcagaatcgt agtcggcctt ggcttgttta aaactttttt tagagtagct ctgtgcttta
     1801 ctcttggagg cttgctgcag gcataaacgg gcatagtcaa catctccaat cggaggttca
     1861 ggtattaact tgtagaatag cacagaaatg ccggtagcca cgaccaaact cgccagtata
     1921 ataacagcta ttcttttcga ttttctactc ataaactcct atttaatagg ctgataacga
     1981 ataaattagg ttatagaaaa atgatttata aaacctaagt aaaccaacta tttccgagca
     2041 acatgaacaa gccact
//
LOCUS       DAOOQX010000372         2046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_68317_length_2046_cov_2.902562, whole genome shotgun sequence.
ACCESSION   DAOOQX010000372 DAOOQX010000000
VERSION     DAOOQX010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68317_length_2046_cov_2.902562"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1569)
                     /gene="mnmE"
                     /locus_tag="PLX53_06485"
     CDS             complement(169..1569)
                     /gene="mnmE"
                     /locus_tag="PLX53_06485"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOY72765.1"
                     /translation="MLDDSTIVAIATPPGMGAIAVIRVSGVDSITLTDGVFKPVMAGK
                     SLLKAKPYTLHYGHIVDMERVIDEVLVSIFHAPHSYTGENAVEISCHGSVVIQQQILE
                     LLIRRGARLAKPGEFTLRAFLNGKMDLSQAEAVADLIASTSEAERRVALQQMRGGFSD
                     KLKLLRDQLLRFTALIELELDFSEEDVEFADRKQLEALVGNIHEEVSRLADSFSMGNA
                     IKQGIPVTIAGKPNVGKSTLLNRLLNEEKAIVSEIAGTTRDAIEDTIQLAGMNFRFID
                     TAGLRHTTDEVESLGIARTHSKIEQAKVVLYLIDAAESMSVGLTEVEKFQQKLTNQQT
                     LMVLLNKADRAEAQHTELLAQNIESHLHCITLIISAKSGQNINELTQNLVNIGMSAQP
                     DGNNVVVTNARHYESLVNARDSLARVLSGLKSNLTGDLLAVDIHQALYHLGSITGEIT
                     NDEVLGYIFSKFCIGK"
     gene            complement(1793..>2046)
                     /locus_tag="PLX53_06490"
     CDS             complement(1793..>2046)
                     /locus_tag="PLX53_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY72766.1"
                     /translation="VENNFATTIWVIPNPTSGNVVIDLGGVYPAISVSITDQSGRNIR
                     NIRGENVDRIEIYLNEPKGIYLLTIQDGKQRATVRIVKY"
BASE COUNT          491 a          491 c          483 g          581 t
ORIGIN      
        1 cctctatttt tgtacctcat ctgcaaacgg tacaacaaag gtacaaattt gtacctaata
       61 aagcaaagta tcacaaaata aataataact ttgcaccaat aaaaagccct tatttcaggg
      121 cttcaagtgg gtttaatact tgttttatgt ttggttgttt tgtggtggtt actttccgat
      181 acagaatttt gagaatatgt agcctagcac ctcatcgtta gttatttcgc cggttatgct
      241 tcccagatgg tagagagcct gatgtatatc cactgccaac aggtcgccgg ttaggtttga
      301 ttttagtcca gataataccc tagcaaggct atcgcgtgcg tttacaagcg attcgtagtg
      361 gcgtgcgttt gtaaccacca cgttgttacc atcgggctgt gctgacatac caatatttac
      421 aaggttttgt gttagttcgt ttatgttttg tcctgatttt gctgaaatta tgagggttat
      481 acagtgaaga tggctttcaa tgttctgggc tagcagctcg gtatgttggg cttcggctct
      541 gtctgccttg ttcagcagca ccataagggt ttgctggttg gtgagttttt gctggaattt
      601 ctcaacttcg gtcaggccaa cgctcatgct ttctgcggcg tcaatcaggt agagcaccac
      661 tttggcctgt tcaattttgg aatgagttcg ggctatgcca agcgactcaa cctcgtcggt
      721 ggtgtgcctg agtccggcag tgtcaatgaa cctgaagttc attccggcca gctgtatggt
      781 gtcctcaatg gcatcgcggg tggtgccggc tatttccgaa acaattgcct tttcttcatt
      841 caataggcgg ttgagcaggg ttgacttccc gacgttgggc ttccctgcta tggtaacggg
      901 gatgccctgc ttaatggcgt tgcccatgct gaacgaatcg gccaaacggc taacctcttc
      961 atgaatgttg ccaacgaggg cttcgagctg tttgcggtcg gcaaactcca catcctcctc
     1021 gctaaagtcg agttctagct caatgagagc ggtaaacctc agcagctgat cgcgcagcag
     1081 cttcaactta tccgaaaacc ctccccgcat ttgctgcaag gccacgcggc gctcagcttc
     1141 actagttgac gctataaggt cggcaaccgc ttcggcctgc gacaggtcca tctttccatt
     1201 caggaacgcc cgcagggtga actcccctgg tttggcaagt cttgccccac ggcgaatcag
     1261 caactccaga atttgctgtt gaataacaac cgagccatgg cacgaaattt caactgcatt
     1321 ctcaccggta taggagtggg gcgcatgaaa aatgcttacc agcacctcgt caatcacccg
     1381 ttccatatca acaatgtgtc catagtgaag ggtgtagggt ttagccttta ggagactttt
     1441 cccggccatc acaggtttga acacaccatc ggtcagggtg atggaatcca caccgctaac
     1501 cctaatcaca gcaatggccc ccattccggg gggtgtggca atggccacta tggttgagtc
     1561 gtcgagcatc gtatattttt tgcaaagtta agctattaga ggatgaaatg aataatcggt
     1621 tggcagccgc catattctaa ctataccgct atccggaaat ggttagaagc ctacactaag
     1681 cttgaaaaag ggctggtctt gaattctgaa ttcaccaaaa aatccccggg ttaaaccccg
     1741 gggattttca atccagttga accctgcaaa aaaagtctgc tacggttttg ctctagtact
     1801 tgacaatcct gacagttgct ctctgcttgc catcctgaat ggtaagcagg taaatccctt
     1861 tgggttcgtt taggtagatt tcgattctat caacattctc acctcgaata ttcctgatat
     1921 tcctaccact ctgatcggtg attgaaacgc taattgcagg gtaaacgcca cccagatcaa
     1981 tcacaacgtt tccgctggtt gggttaggaa taacccagat ggtggtagca aagttgtttt
     2041 caacca
//
LOCUS       DAOOQX010000373         2044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_68412_length_2044_cov_3.039216, whole genome shotgun sequence.
ACCESSION   DAOOQX010000373 DAOOQX010000000
VERSION     DAOOQX010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68412_length_2044_cov_3.039216"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1467)
                     /locus_tag="PLX53_06495"
     CDS             complement(4..1467)
                     /locus_tag="PLX53_06495"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOY72767.1"
                     /translation="MAKVNIKLENLSEMFPNSWTDEQKAKGNTLFLKYLSEKAHKFYG
                     GKVQTVPKAPVPGFNWFNVWYTPGVSKVSTTIRDNNDTSFELTNRGNLVAVVSDSTRV
                     LGDGDCTPPGGLGVMEGKAFLMKYLGGVDAVALCIDSKGKDGKNDPEKIIDFVKMCQH
                     SFGAVNLEDISQPNCYRVLDVLREECIIPVWHDDAQGTACVTLAGLLNALKLANKKMS
                     NAKMVLFGAGAANTTIARLIIADGGDPHNMVIFDSHGGLHKNREDIKADHRFYRKWEL
                     CEQTNPQCINTMEEAMRNADVLISVSTPGPDTIKQEWVKLMAPKSVVFACANPVPEIY
                     PYAAKEAGAYIVATGRGDFPNQVNNSIGFPGILKGALIARASKITDSMAIAAAHSLAG
                     FAEKRGISPENIVPTMDEADVFPVEAADVAVQAVKEGVARRQVSWEEAYRIAKKDIES
                     SRSMTKALYDNGYIANPPAEMLEDALLWTIQQVEAKK"
     gene            1745..>2044
                     /locus_tag="PLX53_06500"
     CDS             1745..>2044
                     /locus_tag="PLX53_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014709327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOY72768.1"
                     /translation="MSILVGKNSRVLVQGFTGSEGTFHATQMIEYGTNVVGGVTPGKG
                     GQKHLDRPVFNTVDDGVKTTGADVSIIFVPPAFAADSIIEAAAAGIKLVVCISEGI"
BASE COUNT          527 a          452 c          455 g          610 t
ORIGIN      
        1 cttttacttt tttgcctcaa cctgttgtat agtccacagg agcgcatctt cgagcatttc
       61 tgctggtgga ttggctatgt acccgttgtc gtaaagtgct ttggtcatgg agcgcgagct
      121 ttcaatatcc tttttggcaa ttctgtaggc ttcctcccag ctaacctgcc ttcgggcaac
      181 gccttccttt acggcttgaa cggcaacatc ggcagcttca acagggaaaa catcagcttc
      241 atccattgtt ggaacaatat tttccgggga aatacccctt ttttcagcga agccagctaa
      301 tgagtgagct gcggcaatag ccatactatc agtaattttt gatgcgcggg caatcaatgc
      361 ccctttcaaa attccaggaa aaccaatcga attgttcacc tggttcggga agtccccgcg
      421 tccggtagcc acaatgtaag cgcctgcttc tttggcagca taggggtaaa tttccggaac
      481 gggattggcg caagcaaaaa caaccgattt gggtgccatt agcttaaccc attcctgctt
      541 tatggtgtcg ggacctggag tagaaaccga aataagcacg tcggcatttc gcatagcttc
      601 ttccatggtg ttgatgcatt gtgggtttgt ttgttcacag agttcccatt tacggtagaa
      661 cctgtgatca gcctttatat cctccctatt tttatgcaag ccgccgtgtg agtcaaaaat
      721 aaccatgttg tgcggatcac cgccatcggc aataatcaaa cgggcaatgg tggtgttggc
      781 tgctccggca ccgaaaagca ccattttggc attactcatt tttttgttgg caagctttag
      841 ggcattaagt aatcctgcta gcgtaacaca tgctgttcct tgtgcatcat cgtgccaaac
      901 cggaatgatg cactcttcac gaagcacgtc gagaactctg tagcagtttg gctgagatat
      961 gtcctcaagg ttaactgccc caaagctatg ctggcacatt ttaacaaaat caataatctt
     1021 ctcgggatca tttttcccat ctttaccctt gctgtcgatg caaagtgcaa cagcatcaac
     1081 tcctccaaga tacttcatca aaaatgcctt tccctccata acccccagac cacccggagg
     1141 cgtgcaatcg ccatcgccta gcacacgggt agaatcgcta acaacagcaa ccagattgcc
     1201 cctgttggta agctcaaaag aggtatcatt gttgtcgcga atggtggtgg aaactttcga
     1261 tactccgggg gtgtaccaca cattaaacca gttaaacccg ggaacaggag cttttggtac
     1321 tgtttgtact ttgccaccgt agaacttgtg ggctttttcg ctcaaatatt ttaagaacag
     1381 cgtattaccc ttagcttttt gctcatccgt ccagctgttt gggaacattt cactaaggtt
     1441 ctctagtttt atgtttactt tagccataac cagttaaatt ttaatgattt gtatgcattg
     1501 cccttttata aaataccctt accaagttac gcaaacattt tgtgatagct attcggaaat
     1561 gctgaaaaat tttatcgtaa acgtttgtaa tgtatataaa aggttagatt tgttagtaaa
     1621 attgttgcag ttttcctata aattggctag gtttgattcg ataatttttt tacttgaatt
     1681 taacttcttg catgcaggtt aaggtaattt ttgataggag ttgtaatcct aaaaatgagg
     1741 aaagatgagt attctcgtag gtaaaaattc cagggtgctg gttcagggct tcaccggtag
     1801 cgagggcacc tttcacgcca cccagatgat tgagtatggt actaatgtgg tgggaggggt
     1861 tacaccaggg aaaggaggac aaaaacatct ggacaggccg gtatttaaca ctgttgacga
     1921 tggagtaaag acaaccggag cagacgtttc aataattttt gtacctcctg cttttgcggc
     1981 cgatagcatt attgaagcag cggcagctgg tatcaaactt gttgtatgta ttagcgaggg
     2041 tatt
//
LOCUS       DAOOQX010000374         2041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_68539_length_2041_cov_3.661631, whole genome shotgun sequence.
ACCESSION   DAOOQX010000374 DAOOQX010000000
VERSION     DAOOQX010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68539_length_2041_cov_3.661631"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLX53_06505"
     CDS             <1..519
                     /locus_tag="PLX53_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOY72769.1"
                     /translation="DDKRLARGWALPGKKGLTHRVGGLEKDFLKGSLSHDPINHQRMV
                     DIRAAKVERVREFIPEQKVIGDSEGDLLVIGWGGTYGHLLDAVTDLRGKGKSVSLCHF
                     SYINPLPKNTRDILSRYKKLVVCELNMGQFANYLRMNFQEFNYLQFNKVQGLPFTVVE
                     LVEKFNQLLEGK"
     gene            522..1553
                     /locus_tag="PLX53_06510"
     CDS             522..1553
                     /locus_tag="PLX53_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOY72770.1"
                     /translation="MEEQYLNYTPQDFKSDQEVRWCPGCGDHAILSAVQKALPEVSKE
                     LKYNIERYVFVSGIGCSSRFPYYVNTYGFHGIHGRASAIATGVKVANPTLSVWQITGD
                     GDALAIGGNHFIHAIRRNIDVNILLFNNQIYGLTKGQYSPTSPLGKITKTSPFGTVEH
                     PFRPGELVIGAQGKFFARTLDVDVKLTSEIMVEAAKHDGTSVVEILQNCVIFNDGAYD
                     FISSKETRDDRVIVLHHGEKMVFGKNRDKGLVLSGLSLKVVTIGQGGFTMDDILVHDA
                     YNPNPGVHMMLANMEFPEYPIALGVIRNVKDSTYDDNVRDQVLEVMKDAKIKCVDDLL
                     NSGETWEVK"
     gene            complement(1682..>2041)
                     /locus_tag="PLX53_06515"
     CDS             complement(1682..>2041)
                     /locus_tag="PLX53_06515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY72771.1"
                     /translation="DYSYNVTKTGYNPILGNTFTVSGGDKNVDILLFPLSAGDSKLNA
                     AAAYPNPFDSYLRIGGAAEVEYIKLTSLTGQVVMEFRGNGSETYTLNTANLPAGFYLI
                     TMESASGEKLVKKVIKQ"
BASE COUNT          530 a          475 c          510 g          526 t
ORIGIN      
        1 gatgataagc gccttgccag aggctgggcg ctgcccggga aaaaaggtct tacccacagg
       61 gttgggggcc tcgaaaaaga ctttctgaaa ggttcgcttt cgcacgaccc aataaaccat
      121 cagcgaatgg tggatattag ggctgccaag gtagagaggg tcagggagtt tatccccgaa
      181 caaaaggtga ttggtgatag cgaaggcgac ctgctggtta tcggttgggg tggcacctat
      241 ggccacctgc tcgatgccgt aaccgacctg cgcggaaagg ggaaaagcgt ttcgctttgc
      301 cacttcagct atataaaccc actgccaaaa aatacccggg atatactgag ccgctataag
      361 aagctggtgg tttgcgagct aaacatgggg cagttcgcca actaccttcg tatgaatttt
      421 caggagttca actacctgca gttcaacaag gttcaagggt taccctttac cgtggttgag
      481 ctggtagaaa agtttaacca attactggag ggaaaataac catggaagaa cagtacttaa
      541 attatacacc tcaggatttc aaaagtgacc aggaagtgcg ctggtgcccc ggatgtggcg
      601 atcacgccat tctgagtgca gtccaaaaag cgctacccga ggtttcaaaa gagctgaagt
      661 acaacataga gcgctacgta tttgtttcgg gaataggctg ctcaagccgc tttccttact
      721 acgtaaatac ctatggcttt catggtattc atggtcgtgc ctcggccatt gctaccggtg
      781 ttaaagttgc caatcccacc cttagcgttt ggcagattac cggcgacggg gatgccctgg
      841 ctattggtgg caaccacttc attcacgcca ttcgacgcaa cattgacgtg aatattctac
      901 tattcaacaa ccaaatctac ggtttaacca aaggtcagta ctcccccacc tcgccacttg
      961 gcaaaatcac caaaacatcg ccttttggca ctgttgagca cccattccgg cctggtgagc
     1021 tggttattgg cgcacaggga aagttttttg cccgtaccct tgatgtggat gttaagctca
     1081 cctccgaaat tatggttgag gccgctaagc acgacggcac atcggtagta gaaattctac
     1141 agaattgcgt aatttttaac gatggggctt acgacttcat cagcagcaag gaaaccaggg
     1201 acgacagggt tattgtcctt catcacgggg aaaagatggt ttttggcaag aatcgggata
     1261 agggactagt gctcagcggg ttgagcctaa aggtggtaac cattggacag ggaggtttca
     1321 ccatggatga tatcctggtt catgatgcct ataaccccaa ccccggcgtt cacatgatgc
     1381 tggccaacat ggagtttcct gaatatccga ttgctcttgg cgttatcagg aatgtaaagg
     1441 attcaaccta cgacgataat gttcgtgacc aggtgctgga ggtaatgaag gatgcaaaaa
     1501 tcaaatgcgt tgacgacctg ctcaacagcg gcgaaacgtg ggaggttaag taggatacct
     1561 gcgctgcatt taatattgat ttaatttttt acttgtacaa ctacgaccgg ggggttcccc
     1621 ggtcgttttc attttatttc aacacctaag attcaacccc aatgggtgtg aaatacatct
     1681 actactgctt gattaccttt ttcaccagct tttcgccgct ggctgactcc atggtaataa
     1741 ggtaaaatcc ggccggcagg ttagctgtgt tgagtgtata ggtctccgat ccgtttcctc
     1801 taaactccat caccacctgc ccggtaaggc ttgtaagctt gatatattcc acctctgctg
     1861 cgccgcctat ccgtaagtag ctgtcaaatg ggttggggta agctgctgct gcatttagtt
     1921 tggaatcgcc agccgacagg gggaaaagta aaatatctac gtttttatcc cctccggaaa
     1981 cagtaaatgt attccccaga attgggttat atccggtttt ggtaacattg tagctataat
     2041 c
//
LOCUS       DAOOQX010000375         2036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_68801_length_2036_cov_3.378092, whole genome shotgun sequence.
ACCESSION   DAOOQX010000375 DAOOQX010000000
VERSION     DAOOQX010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68801_length_2036_cov_3.378092"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(453..1289)
                     /locus_tag="PLX53_06520"
     CDS             complement(453..1289)
                     /locus_tag="PLX53_06520"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOY72772.1"
                     /translation="MITVRRVIPAQQVTDGAGVKLNRVFGYDTRMETDPFLLLDYFGS
                     ENPTDYAAGFPWHPHRGIETITYMLQGKVEHSDSLGNRGEVNPGGVQWMTAGSGIIHQ
                     EMPKPISGLMYGFQLWLNLPRSFKMVPPTYHDLKSSDIPEVETSSSRVRVIAGRFRKA
                     VGLSTPYNSSIDVLDVRLWPDALFDEALNANSSYFLFVYDGQISFADYSKPIGSLNGV
                     ILDKGSILQLKAESNGANFLLFGGQPINEPIAWRGPIVMNTNDELSVAFEEINQGTFI
                     KE"
     gene            1522..>2036
                     /locus_tag="PLX53_06525"
     CDS             1522..>2036
                     /locus_tag="PLX53_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY72773.1"
                     /translation="MTKLRAFILSLTLAAAFSLAHGQETSISTREEALFAKAFELYQK
                     GLYASSQKTFQKFLSEYSSDVVAQLRTDAEFYSAICAIELNNNDAEYLLGRFIQNHPE
                     SQYIDLAYFVMGKRQYQQKNFTSSIYWLSMIDGEGLDREKRYEMQFMLGYCYFMTNDF
                     DLPALSIAELYR"
BASE COUNT          570 a          475 c          439 g          552 t
ORIGIN      
        1 agtgccgctt gggcattccc gcgctacgtt ccgcccccga cccatttgcg ggtcatgctt
       61 ttatgccgtt ttacttatac cagcaacgtt caacgtttcc cgcctttacg agcaccgcgg
      121 agaataccct tcccaagttg gggctgaagg caagggtaaa ggttaaagaa taagaatgtt
      181 caaggttaag aactttttta ttaaaagagt ttttaagccc cgaaggggct agagagccta
      241 gcccccgggc aaagccctgg gttttaaggt ggccacgggt atgttggcga tgcaagtagg
      301 agcctctcta aagaaccatt gagacctttt gcatcgcaac aggaaaaaat aaggtttata
      361 tcacacccct tcaggaattc agggaaaaat tgcacgaagt gaaatcaata ccaacaatac
      421 tcctctgccc gttaaatctc agagcaaaaa acttactcct taatgaatgt accctggttt
      481 atctcctcaa atgcaacgct gagctcatcg ttggtgttca ttactatggg gcctcgccat
      541 gctatgggtt catttatggg ttgaccacca aataagagaa agttggcacc attactttca
      601 gctttaagct gcagaataga tcccttgtcg agtataacac catttaagct gccaataggc
      661 ttagagtaat cggcgaacga gatttgccca tcgtaaacaa acaggaagta gctggagttg
      721 gcattcagcg cttcgtcaaa aagggcatcg ggccatagcc taacatcaag aacatcaatg
      781 ctgctgttgt agggtgtact taatccaacg gccttacgga atcgaccggc aattacccta
      841 accctcgatg agcttgtttc aacctccggt atatcggatg attttaaatc gtggtaggta
      901 ggaggtacca ttttaaagct acggggaaga ttcagccaaa gctgaaaacc atacattaac
      961 cccgaaatgg gcttaggcat ctcctgatgg attattccgc tacctgcagt catccactgt
     1021 acaccgcccg ggttaacttc acccctattg ccaaggctgt cggaatgctc gacctttccc
     1081 tgtagcatat aggtgatggt ttcaattcca cgatgggggt gccatggaaa tccagcagca
     1141 tagtcggtag gattttcgga accaaagtaa tccagcaaga ggaagggatc agtctccatt
     1201 cttgtgtcat acccgaaaac cctatttaac tttactcctg caccatcggt aacctgctgg
     1261 gcgggaataa ctctccttac cgtaatcacc acctgcttat tttagaccgt taagttagta
     1321 aatattttga taatgtcaaa ttaaaactct tatggcaagt ttttaacgtg catattgtag
     1381 aaccaggagc gcttttactt actatcagac tattttttac aataacactt acacccaatg
     1441 ctttgttgac aaaaaaatga agagccagta ggcagttttt ccatttctta ctacttttat
     1501 cggaaattaa gatttgatgg tatgacaaaa ctaagggcgt ttatactttc tttaacgcta
     1561 gctgctgcct ttagccttgc tcacgggcag gaaacaagca taagcacccg tgaggaagct
     1621 ctttttgcaa aagcctttga gctttaccag aaggggttgt atgccagctc gcagaaaaca
     1681 ttccagaagt ttctgtctga atattcctcg gacgttgtgg cccagctaag gaccgatgca
     1741 gaattttaca gcgccatttg tgccattgaa cttaacaaca acgatgctga gtaccttctt
     1801 ggccgcttca ttcaaaatca tcccgaaagc cagtacattg atttagccta ctttgtcatg
     1861 ggcaagcggc agtaccagca aaagaacttt accagctcaa tctactggct atccatgatt
     1921 gacggggaag gactagacag ggaaaaacgc tacgagatgc agttcatgct gggttactgc
     1981 tactttatga cgaacgattt tgatcttcct gccttatcaa ttgcagagct ttaccg
//
LOCUS       DAOOQX010000376         2035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_68832_length_2035_cov_3.784343, whole genome shotgun sequence.
ACCESSION   DAOOQX010000376 DAOOQX010000000
VERSION     DAOOQX010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68832_length_2035_cov_3.784343"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            549..2015
                     /gene="amrB"
                     /locus_tag="PLX53_06530"
     CDS             549..2015
                     /gene="amrB"
                     /locus_tag="PLX53_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOY72774.1"
                     /translation="MESSCKGQTGGSSRGVDRQPAVAGSFYPASREQLVKDLQGYFDA
                     AEVSLTSQPIALIVPHAGYVFSAPVAAAGFKQLDRERKFKHIFLIGSSHTMYFNGVSA
                     YTQGHFITPLGRVKVDDLAKELADRYDFINDDPMPHEREHGLEVQLPFLQYWLKHEFC
                     IVPLIIGGESQSTSTLLAEVLAPYLNEENLFVISSDFSHYPTYAEATKSDKSMADAIV
                     SNSSSAFMKAKLNSENSGADNLVTAMCGWTSGLTLLKMTEKIKGITYQPLIYMNSGDS
                     PYGEKDRVVGYWAIAAVRESNRSGDFSLTEEDKMELLKLARKTITDHLNGLKPTPPDL
                     QNLSANLKTNAGAFVTLRRDGQLRGCIGSFSTTTPLYQVVMAMAVAAATEDYRFPPVT
                     RNELGEVEIEISVLTPMKKISSIDEIELGRHGIYIRKGGHSGTFLPQVAAETGWSLEE
                     FLGHCARDKALIGWDGWRDAEIFTYEAIVFEEKDFKMN"
BASE COUNT          536 a          462 c          480 g          557 t
ORIGIN      
        1 taaacgagga tacacctatg agctgttcta actcggcatt tctaaccaac cttaagccac
       61 aatggttgct agcagcccag agtagtttat ttaggttaag cgtggtggac agtgcaataa
      121 acataaaagg cctgccctgc tcaacagtaa gtatttcctt cttttttgcc ggcattttag
      181 cttactagtc tagcacaaat ataaccctgt ttttaaacaa tcttaccgaa cccctaaatt
      241 tatcttaacg aaatcggacg gtgttggaag ctgaatgcga acgggttaat ttttagcatt
      301 tctgaatttc aactcctcgc ttaccctatt cccgaccaac cgttagcctt ctaaccatcg
      361 ggaatttgaa taggttttag caacttttcc caaagttgtc ttatggtagc tggctggagt
      421 gatatgaact tgatttttct tttattttga aataactttg aatgggaaag gtaaatattc
      481 gtgctatcat gccgggcttt caatcacgat attttgtaac gataagttta ctaataacac
      541 tagtagcaat ggaatcgtca tgtaaagggc aaacgggtgg aagtagtagg ggagttgacc
      601 gtcaacctgc agttgccgga tcgttctacc cagcctccag ggagcagctg gtaaaggatt
      661 tgcagggtta ttttgatgcg gctgaggtta gccttacatc tcaaccaata gctttaatag
      721 ttccccatgc cgggtacgtt ttctccgcgc ctgttgcagc tgcaggtttt aagcagctcg
      781 atagggaaag gaagtttaag catatttttt tgattggctc cagccacacc atgtacttca
      841 acggtgtatc agcatatacc caaggtcatt ttatcacccc ccttgggcgt gttaaggttg
      901 acgacctggc aaaggagctg gccgataggt acgattttat caacgacgat cccatgcccc
      961 acgagcggga gcatgggctg gaggtgcagc tcccctttct ccagtactgg cttaagcatg
     1021 aattctgcat tgttcctctt atcatcggtg gtgaatcgca aagtaccagc accctgctcg
     1081 ccgaagttct tgccccatac ctaaacgagg aaaacctgtt tgtaataagt tcagactttt
     1141 cgcattaccc gacatatgct gaggccacaa aatccgacaa aagcatggcc gatgcaattg
     1201 tttcgaactc gtcttcagca ttcatgaaag ctaagctgaa ctctgaaaac tctggtgctg
     1261 acaatctggt taccgccatg tgtggttgga cttcggggct aaccctgctg aagatgaccg
     1321 aaaaaattaa agggattacc taccaacccc tcatctacat gaattcgggt gattcgccat
     1381 acggcgaaaa agatagggta gtgggttact gggccattgc tgccgtgagg gaaagcaata
     1441 ggtctggtga tttttctttg accgaagagg acaaaatgga gctgcttaag ctggcgcgta
     1501 aaaccattac cgaccacctc aacggcttaa agccaacacc tcccgatttg cagaacctga
     1561 gcgctaacct gaagaccaat gccggtgcat ttgttactct tcgcagggac ggacagctta
     1621 ggggttgtat tggcagcttc tctactacta ctccactcta ccaggtggtt atggccatgg
     1681 ccgttgctgc cgctacggag gattaccgtt ttccccctgt aacccgtaat gagcttggtg
     1741 aagttgaaat tgaaatttcg gtgcttactc caatgaaaaa aattagcagc attgacgaga
     1801 tagagctggg caggcatggc atatacatcc ggaaaggcgg acactccgga acatttctcc
     1861 cacaagttgc cgccgaaaca ggctggagcc ttgaggaatt tcttggccat tgtgcacgcg
     1921 acaaggctct tattggctgg gacggatgga gggatgccga gatttttacc tacgaggcca
     1981 ttgtgtttga ggaaaaggat tttaaaatga attaaatctg ccggttatga atatg
//
LOCUS       DAOOQX010000377         2035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_68844_length_2035_cov_2.790909, whole genome shotgun sequence.
ACCESSION   DAOOQX010000377 DAOOQX010000000
VERSION     DAOOQX010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68844_length_2035_cov_2.790909"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2035
                     /locus_tag="PLX53_06535"
     CDS             <1..>2035
                     /locus_tag="PLX53_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOY72775.1"
                     /translation="LEEPSVLPTKIPLLLVNGTSGIAVGMATNMPPHNLGEVVDAICA
                     TIDSPDITTDELLKYVKGPDFPTGGVIYGTQGVKEAYRTGKGKIVVRSKIEIEQTESG
                     RSKIIVTEIPYMVNKAEMIRRIADLINEKKIDGISYINDESDRDGMRVVIILKKEAVA
                     NVVVNNLFKHSQLQSSFAINNIALVDGRPKLLNLKDLIVHFIKHRHDVIIRRSRFELE
                     EAEKRAHILEGLLIAIDHIDEVINLIRSAQSPAEAKEKLVERFGLSDIQSQAIIDMRL
                     RSLTGLEREKVKEEYNELQKLIAYLKQVLEDKALQMKIIKDELVEVKQKYGDARKTEI
                     IPDAEEFNPEDFYADEEVVITISHMGYIKRTPLYEYKLQSRGGVGAKGSDTRDEDFIE
                     HLYVATMHNTMLFFTENGKCFWLKVYEIPEGTKSSKGRAIQNLINIENDDVVRAYINV
                     KGLDDTDYLKNNYIVLCTKRGIIKKTPLEAYSRPRQNGVIAVTVKEGDQLIEACLTDG
                     KSHILIAARNGKAIRFPEHLVRPIGRTGSGVRAISISPDDEVVGMVCVEENASKDILV
                     VSENGYGKRSRLEDYRITNRGGKGVKTINITPKTGKLISIKAVDENNDLMIINRSGLT
                     IRLKISELRVMGRNAQGVRLINLKKNDSIAAIANVESSENEPDLKAIRERFIRI"
BASE COUNT          644 a          387 c          489 g          515 t
ORIGIN      
        1 ccttgaggag ccatctgttt taccaactaa aattcccttg ttgctggtta acggcacatc
       61 gggtattgcc gttggaatgg ctactaacat gcctcctcat aacctgggcg aagttgttga
      121 cgctatttgt gctacaatag atagtcctga tataactact gatgagctgt taaagtatgt
      181 taaaggtcct gatttcccaa ccggaggggt tatttatggc acgcagggcg ttaaggaagc
      241 ctaccggaca ggtaagggta aaattgtggt tcgttcaaaa atagaaattg agcaaaccga
      301 gtcgggaagg tccaaaataa ttgtaaccga aataccctac atggtaaata aggccgagat
      361 gatccgtcgg attgccgacc ttataaacga gaaaaaaatt gatggtatta gctacatcaa
      421 cgatgagtcg gatagggatg ggatgagggt tgtaataatt cttaaaaagg aagcggttgc
      481 taatgtcgta gtaaacaacc tttttaagca ttcacagctg cagtcatcat ttgccatcaa
      541 caacattgcc ctggttgatg gaaggccgaa gctgttgaac cttaaggacc taattgttca
      601 ctttatcaag caccgacatg acgtaattat tcgcaggtca aggttcgaac tggaggaggc
      661 cgaaaaaaga gcccacatac ttgaaggcct gcttatagcc attgatcata ttgacgaggt
      721 tattaacctt atccgttcag cccaatcacc ggccgaagcg aaggaaaaac tagttgaacg
      781 atttggcctt tcagatattc agtcccaggc tattattgat atgcgcctcc gcagccttac
      841 aggacttgaa cgtgagaaag taaaggagga gtacaatgag ctccaaaagc tgatagccta
      901 tcttaaacag gtgcttgagg ataaagccct gcaaatgaag attattaagg atgagctggt
      961 tgaggtaaag caaaagtatg gagatgcccg aaaaacagag ataattccag acgccgagga
     1021 gtttaaccct gaagattttt atgccgatga ggaagttgtc ataaccatct cgcacatggg
     1081 ctacattaaa cgaaccccgc tttatgagta taagctccaa tcgaggggtg gtgtaggggc
     1141 taagggtagc gatacacgtg atgaggattt tattgagcac ctgtatgttg ctactatgca
     1201 caataccatg ctgtttttta ccgaaaacgg caaatgtttc tggctaaaag tttatgaaat
     1261 tcctgagggt actaaatcat caaaaggaag agctattcag aatctcataa acattgaaaa
     1321 tgacgatgta gttagagcct acatcaatgt caaagggctt gatgacacag attatctcaa
     1381 gaacaactac atcgtgctgt gtaccaaaag aggcattatt aaaaaaacgc ctcttgaggc
     1441 ttacagccgg ccacgtcaaa acggtgttat tgcggtaacc gttaaggagg gcgaccagct
     1501 tattgaggct tgcctcacag atggcaaaag ccacatactg attgctgcaa gaaatggaaa
     1561 ggccatacga ttccccgaac atcttgtccg cccgataggt agaactggta gtggggttag
     1621 ggcaatttct atttcccctg acgacgaagt ggttggcatg gtttgtgttg aggaaaatgc
     1681 atcaaaagat atccttgttg tttctgaaaa tggttacggc aagcgatcca ggttggagga
     1741 ctatagaata accaacaggg gtgggaaagg ggtgaaaacc attaacatca cccctaaaac
     1801 agggaagctt attagcatta aagcggttga tgaaaataac gacttaatga ttattaatcg
     1861 cagcgggctt accataaggc ttaaaatcag cgagcttagg gttatgggtc gaaatgccca
     1921 gggagttagg ctgataaacc taaaaaagaa tgatagtatc gctgcaatag ccaatgttga
     1981 atcatctgaa aatgaaccag atctaaaagc tattagagaa cgttttattc gcatt
//
LOCUS       DAOOQX010000378         2033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_68941_length_2033_cov_3.300809, whole genome shotgun sequence.
ACCESSION   DAOOQX010000378 DAOOQX010000000
VERSION     DAOOQX010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68941_length_2033_cov_3.300809"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1381
                     /locus_tag="PLX53_06540"
     CDS             134..1381
                     /locus_tag="PLX53_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72776.1"
                     /translation="MIKYAISKADQNLKGEITLSPSKPMIHKFLVIRILKSSNLTQRL
                     TTESEDAQIVNSEVINEGIKKNRGTSAKALRHIRAFLNYFGGEWILSSSNILKDRSTE
                     RIVKVLQKSGLNVSYEKRSGRPPFRLTGKNLSGIIQRVDGSINSKIIESKLLFAPTAS
                     IDQIEEVCNRILSSNYVGTTLRALQYLGVNTDWKEDEVLVEHEINDGSEMVIEPDWSL
                     ASCWYQMVALAQKGELQINGLKIDTFQNESAIRHIYNDFGISTLIMPDGISIKRKGKL
                     TGKFTHDFTSYPNLLPCAITTCVGLGIPFEMKGIESLNAADPNRMQLLTEELVKVGAK
                     VELKSINGTQVATFNGTSGLSRLKSVEFESKNDYRILLALVSIAVRGIQVIIDSPMVT
                     NKAYPSFWDDMKKLGFVVNSVTE"
     gene            1778..>2033
                     /locus_tag="PLX53_06545"
     CDS             1778..>2033
                     /locus_tag="PLX53_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72777.1"
                     /translation="MRNKKVLRLGLLGLLATMMVASCSKEEDERLQEPISNDWTVRPD
                     FATLDTRPQDVIASFTLTEKDAELVKPDENAVKGAKYALVI"
BASE COUNT          613 a          454 c          442 g          524 t
ORIGIN      
        1 aagtaccttc gcgatagcct atcaagctga taatcaactg tcccatgaat tatgatatgg
       61 tagcttgcat taattagctt cattttactt acatttaccg ctgggaccat atcataattt
      121 aattcgatgc accatgatta agtatgccat ttcgaaagcc gatcaaaatt tgaaaggtga
      181 aattaccctt tctccctcca aacccatgat ccacaagttt ttggttattc gcattctcaa
      241 aagctccaac cttacccagc gactaaccac cgaatcggag gacgcacaaa tagtaaacag
      301 cgaggtaatc aacgagggaa ttaaaaagaa tcggggaacc tcggcaaaag ccttaaggca
      361 tatcagggcc ttcctcaact actttggcgg cgagtggatt ctctcatcat caaacatcct
      421 taaggataga tcaacagaga gaattgttaa ggttcttcaa aaaagcggtt taaacgttag
      481 ctacgagaag cgtagcggca gaccgccttt ccggcttacc ggcaagaacc tttccgggat
      541 cattcaacgg gttgacggat ccattaacag taaaattatt gagagcaagc tgctatttgc
      601 ccctaccgcc agcattgacc aaattgaaga ggtgtgtaat agaattctta gctccaacta
      661 cgttggtacc acccttcggg cacttcagta cctgggtgtt aacaccgatt ggaaggagga
      721 cgaggtgctg gtggagcatg agataaacga tgggtcagag atggttattg aacccgactg
      781 gtcgttagct tcgtgctggt accaaatggt tgcccttgcc caaaagggtg agctgcagat
      841 taacgggctg aaaattgaca cattccagaa cgaatcggct attcggcata tctacaacga
      901 ttttggcatt tcaaccctaa ttatgcccga cggcatcagc attaagcgaa agggtaagct
      961 gaccggtaag tttacccacg attttaccag ctatcccaat cttctcccat gcgctattac
     1021 aacctgtgta ggtctgggta ttccatttga gatgaaggga attgaaagcc ttaatgctgc
     1081 agaccccaac aggatgcagc tcctcacgga agagcttgtc aaggtaggag caaaagtaga
     1141 gctgaaaagc attaacggaa cccaggtggc aacctttaac ggaacctcag gcctaagccg
     1201 gcttaaaagt gttgagtttg agtcaaaaaa cgattaccgc atactacttg ctctagtctc
     1261 tattgcagta agaggcattc aagtgatcat agacagcccg atggtaacca ataaagctta
     1321 ccctagcttc tgggatgata tgaaaaaact agggtttgtg gtaaatagtg ttaccgaata
     1381 atagctatta ttcagagaac cccttgggct gccaaatcaa cccttaaaaa aaccgggctt
     1441 tttcgtcata aaaggcaggc tcattgctgt atgcgtcggt tagaattttt taacagcagg
     1501 taggttggct acccgctact ttgccatgca tggaagccgg ctaacggcca ttacaccaac
     1561 tccaccataa cttccggtaa gctgcaggta tttctactct aaaaattacc attttgtttt
     1621 atatgcagcg attccggtta ttcgtgcgta gcaagccgtc gtttgtcgca aaatcagctt
     1681 ttgtttgcgt aatcaaccta actttcggat gttgaatttc attttcaaca aaagatcaac
     1741 tcaaaccaat taaccaaacc aacaaattaa aagtgctatg agaaacaaaa aagtgttaag
     1801 gctagggtta ctaggcctac ttgccaccat gatggtagct agctgctcaa aggaagaaga
     1861 tgaacgtttg caggagccaa tctcaaacga ttggacagta cgtccggatt ttgctactct
     1921 agatacccgt ccacaggatg ttattgctag cttcaccctc accgaaaagg atgcagaatt
     1981 ggtaaaacct gatgaaaatg cagtaaaagg tgccaagtat gcactggtga ttg
//
LOCUS       DAOOQX010000379         2031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_69070_length_2031_cov_3.095648, whole genome shotgun sequence.
ACCESSION   DAOOQX010000379 DAOOQX010000000
VERSION     DAOOQX010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69070_length_2031_cov_3.095648"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1799
                     /locus_tag="PLX53_06550"
     CDS             <1..1799
                     /locus_tag="PLX53_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HOY72778.1"
                     /translation="FTNGWKQARHFRANMDTLVALSTSIAYLFSLFNTIYPEYWTSRG
                     LQGHIYYESASIIVAFILLGKWLEEKAKNQTTSSIKKIMGLQPDMSWLVEGESLSEVP
                     LSRIRKGDVVLVKPGSRVPVDGRVVDGQSVVDESTISGESIPVVKKVGDKAWAGTMNQ
                     RGSFKLIAEQVGSESVLGRIIATVESAQNSKAPVQHLVDKVAGIFVPAVMLVALLTLI
                     TWLVLDPANGFSHGLISMVTVLAIACPCALGLATPTAIIVGVGRAAEKNILIRDAESL
                     ETAHQVNYIVLDKTGTLSEGVPSVDRIEWFTHETTYPGILKFIEQNSGHPLADAIVRH
                     FETADSSGFVADSYESIAGKGVKTTVKGKKYLVGNPDFLRQEGVMFTDTIESSLSEIL
                     NNAGSVVGFAENNLLLCLIRVSDRIKPSSREAIKRLKARNIEVAMLTGDNRANAEFMA
                     RELEIAHYAGQQLPSDKADFIKTLKKQGKVVAMVGDGVNDSEALSVADVGIAMGKGSD
                     IAIDVAQVTILNSDLNMLVTLLDISGKTIRTIRQNLFWAFIYNIIGIPIAAGLLYPFY
                     GITLDPMFAGMAMALSSVSVVTNSLRLRTARI"
BASE COUNT          549 a          452 c          541 g          489 t
ORIGIN      
        1 tctttactaa tgggtggaag caggcccggc atttcagggc aaacatggat accctggttg
       61 cgctaagcac ctcaattgcc tacctcttta gtctttttaa cactatttac ccggagtatt
      121 ggacaagtcg aggccttcag gggcatatct actatgaatc ggcttctata atagtagcct
      181 ttatactgct aggaaagtgg cttgaagaga aagcgaagaa tcaaaccacc tcatcaataa
      241 aaaaaatcat gggcttgcaa cccgatatgt cctggttggt tgagggagag agcctgagcg
      301 aggtaccgct atcccgtatt aggaagggcg atgtagtgct ggtgaagcct ggtagtagag
      361 tccctgttga tggaagagtt gtggatggcc aaagcgtggt tgatgagagc accatttcgg
      421 gcgagtcaat acctgttgtc aaaaaagtgg gtgataaagc atgggccgga acaatgaacc
      481 aacggggcag ctttaagttg atagccgaac aggttgggtc ggaatcggtg ctgggtagaa
      541 tcatcgctac agttgaaagc gcacagaaca gcaaagctcc ggtgcagcat ctggttgaca
      601 aggtggcagg aatatttgtt ccagcggtaa tgctggttgc cctacttacc cttattacct
      661 ggcttgtgct agatccggct aacggcttct cacatgggct aatctccatg gttaccgtgt
      721 tggccatagc ttgtccgtgc gccttgggtc tggcaacgcc aacagccatt attgttggag
      781 ttggacgggc agcagaaaaa aacatcctga ttcgggatgc cgaaagcctc gaaacagccc
      841 accaggtaaa ctacattgtt ctcgataaaa cagggacgct gagcgaaggc gtgcccagcg
      901 tggatagaat cgaatggttt acccatgaaa ccacctatcc gggtatttta aaatttattg
      961 agcagaattc gggtcatcca ctggccgatg ctattgttcg gcatttcgaa acagccgact
     1021 cctcgggatt tgtggccgat agctacgagt ccattgccgg taagggggta aagacaacag
     1081 taaagggaaa aaagtatttg gttggcaatc cagactttct taggcaggaa ggagtgatgt
     1141 ttaccgatac aattgaaagc agcctttcgg agattttaaa caacgccgga tcggttgtag
     1201 ggtttgccga aaataacctt cttctgtgcc tgattcgggt atcggacagg ataaagccct
     1261 cctccaggga agccataaaa agattaaagg ccagaaacat agaggttgcc atgctcacag
     1321 gcgacaaccg tgcaaatgcg gaatttatgg cacgggaact cgagattgcc cactatgcag
     1381 ggcagcagct tccgtcggat aaggctgatt tcatcaaaac ccttaagaaa cagggaaaag
     1441 tggttgccat ggtgggtgac ggcgttaacg atagcgaggc gcttagtgta gctgacgtag
     1501 gaatagccat gggcaaaggc tctgacattg ccattgacgt ggctcaggtt actatcctta
     1561 actccgattt aaacatgctg gtaacgctgc tcgatatttc aggcaaaact atcaggacaa
     1621 tccgccagaa ccttttctgg gcatttatat acaacatcat tggtattcca attgcagcgg
     1681 gacttcttta cccattctac ggaataaccc tagacccaat gtttgcaggt atggccatgg
     1741 ctctcagctc ggtgtcggtt gtaacgaaca gccttcggct gagaaccgcc aggatttgaa
     1801 aaaggacaaa gtgagtttgt acaactaatg actgattcaa tataaaaagg ataaaggtta
     1861 aaaaacttgt ccgcctaccg gcggaacttg tccggctcct gacggattca agaacttgtc
     1921 cgcctaccgg cggaacttgt ccagctcctg acggatcttg tccgcctacc ggcggatttt
     1981 agccccgaag gggcgtaata agtagcaagg ggcgcagccc cttgtatgga g
//
LOCUS       DAOOQX010000380         2021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_69606_length_2021_cov_3.109359, whole genome shotgun sequence.
ACCESSION   DAOOQX010000380 DAOOQX010000000
VERSION     DAOOQX010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69606_length_2021_cov_3.109359"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="PLX53_06555"
     CDS             complement(<1..157)
                     /locus_tag="PLX53_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800285.1"
                     /note="catalyzes the formation of oxalozcetate and
                     L-glutamate from L-aspartate and 2-oxoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOY72779.1"
                     /translation="MPKISEKGLAMPASPIRKLIPFAEEAKRKGRKVYHLNIGQPDIP
                     TPQVALEA"
     gene            complement(183..599)
                     /locus_tag="PLX53_06560"
     CDS             complement(183..599)
                     /locus_tag="PLX53_06560"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOY72780.1"
                     /translation="MKRIFFAILLAAACSTMVNAQSTEKVDENAPEITFTSTEYDYGV
                     VAVNSDGTRKFEFTNTGKQPLILSNVSASCGCTVVEWAKDPVKPGEKGTITVKYNTAI
                     VGTFQKSIRVFSNAKTSPVMLVIKGEVKPNEPAPHQ"
     gene            complement(1186..>2021)
                     /locus_tag="PLX53_06565"
     CDS             complement(1186..>2021)
                     /locus_tag="PLX53_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOY72781.1"
                     /translation="PAGEEYVANDIYLDTTEQQIIIVTGPNMAGKSALLRQTALIVLM
                     AQIGCFVPAQAASIGLVDKIFTRVGASDNLSLGESTFMVEMQESASILNNLSSRSLVL
                     LDEIGRGTSTYDGISIAWAIVEYLHEHPNARAKTLFATHYHELNEMESTFPRVKNYNV
                     SIKELNDRVIFLRKLVRGGSEHSFGIHVARMAGMPPSVIKRAYEILAELEKSQQNETL
                     HKPVAEIGTRREGYQLSFFQLEDPVLKQIRDQIKTLDINNLTPLEALNKLNEIKRLTG
                     L"
BASE COUNT          485 a          436 c          505 g          595 t
ORIGIN      
        1 tagcctcaag cgctacctga ggagtaggaa tatcgggctg tccaatattc aggtggtaaa
       61 ccttacgccc ctttcgtttt gcctcttcgg caaaaggaat cagctttcgg attggagaag
      121 ctggcatagc cagccccttt tcggatattt ttggcatgtc tttatgtgat ttggtttgta
      181 atctattgat gtggtgcagg ctcgtttggt ttaacctcac ccttaattac tagcataacc
      241 ggactggttt tggcgttgga gaaaacgcgt atgcttttct ggaatgttcc aactatggct
      301 gtgttgtact taacggtaat ggttcccttt tcgcctggct taacaggatc cttggcccac
      361 tccacaaccg tgcagccgca tgatgccgaa acattactta aaatcaaggg ttgtttcccg
      421 gtgttcgtaa actcgaattt acgggttcca tctgaattta cggcaactac cccgtagtcg
      481 tactccgttg atgtaaaggt aatttcagga gcattttcgt ccaccttttc ggtgctttgt
      541 gcattaacca ttgtgctgca agctgcggcc aacaaaatgg cgaaaaaaat tcttttcatc
      601 gtaatctcgt tttagcaaaa agttaaacaa tatattgtaa ggcacacttt ttatcagagg
      661 agctttaggc tgagttgaaa ttgcacgaag ttggcagtgc atttcaggtt gcaaagttag
      721 ggttctttta gctatttggt ttaaactact gttaataaat gtaattgtac aatcccacag
      781 ctgggccaag gctacaactt gataaagaag tgcactgggt tgggcaaagt ttagcaaagc
      841 gttcatccgt cgagctattc ttttaggctg cgaccatttt tttagttgtt ttatgtttgg
      901 ctgaggctat aaaaagtaat aaacaattcg tagtctgcgg ttggttgcac cgccgttggg
      961 ctatcgaaag cgggatcagc ctgctattct tgaaatgaca tacggtggaa aacttacaat
     1021 tagctatatt tacagggcag agcccgaaga gatagatacg accgaggcac acagcctaat
     1081 ccaaacaggg aatctgctag atctactttt ctgaaatcaa gggtaggttt ctttggtttt
     1141 tatgggatgc tcggatgtta gcagatgtag aatagcaatc ggtggctaga ggccggtaag
     1201 ccgcttaatt tcattcagct tgtttagcgc ctccagcggg gtaaggttgt tgatgtccag
     1261 cgttttaatc tggtcgcgaa tctgcttcag cacggggtcc tcgagctgga agaagctcag
     1321 ctggtaaccc tccctccggg ttccaatttc tgctaccggt ttatgtagag tttcattctg
     1381 ttgcgatttt tcaagttctg caagtatttc gtaggccctt ttaatcacgc tgggtggcat
     1441 gcctgccata cgagcaacgt ggatgccaaa actgtgctcg cttccgccgc gaacgagctt
     1501 gcgcaaaaag attacccggt cgttgagttc ctttatggag acgttgtagt tcttcacacg
     1561 ggggaaggtg gactccattt cgttcagctc gtggtagtgg gtggcaaaaa gggttttagc
     1621 tctggcgttg ggatgctcat gcaggtattc cacaatagcc catgcaatgg aaatgccatc
     1681 gtatgttgag gttccccgac ctatttcgtc aagaagcaca aggctacggg acgagaggtt
     1741 gttgagaata ctggcgcttt cctgcatttc gaccataaat gttgactcac cgagcgagag
     1801 gttgtcggag gcaccaaccc gggtaaaaat tttatcaacc agcccaatgc ttgcagcttg
     1861 cgccggaaca aaacagccta tctgggccat gagaacaatc agggcggttt gcctgagcaa
     1921 ggctgatttt cctgccatat ttggtccggt aacaataata atctgttgct cggtagtgtc
     1981 gaggtagatg tcgttggcca cgtactcctc gcctgccggt a
//
LOCUS       DAOOQX010000381         2020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_69687_length_2020_cov_1.843257, whole genome shotgun sequence.
ACCESSION   DAOOQX010000381 DAOOQX010000000
VERSION     DAOOQX010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69687_length_2020_cov_1.843257"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1805
                     /locus_tag="PLX53_06570"
     CDS             150..1805
                     /locus_tag="PLX53_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOY72782.1"
                     /translation="MHLKRLSLLILFTAGLRLSLWAQPNRQIPSEKPRLIVEIVASQM
                     RFDYLTRYWDKFSDGGFKMLVNDGASCKNARYSYLLTQAYPGIATISTGANPSIHGII
                     SDSWFSRVNGEVVDAVADEKTTTTGGSFASGRASAKNLVTSTLGDEICLNNPQSKVVS
                     IALDAGSAILMAGHSASGIYWLDTEKGGWVSSSYFSDSLPAWVDTLNAKSLAKLYAEK
                     EWKAINPIQAYEETDTLTARTPEGKKRVSDKIKLLIDGIIGKSEPATSYKKLLETPFG
                     NSLTKDMAIASILGESLGSDENTDLLCITFSANRYITRKFGPHSIEMEDTYLRLDREL
                     AHFLQFLATQVGKENVLVVLTSDQGVSSTPEFLEKSKIPGGYFDPKKAMVLLGSYLNA
                     LYGQGSWITGYHEKQIYLNRRLIEDSNINLAEMQQKVADFMLEFSGVANSATAHTLQS
                     ANFANGIMVKFQNSYNQRRSGDVIINLEPGWVERNGSITSGNSAYDYDTHVPLIWYGW
                     KVKRRVITSPVNIYDIAPTLSSLLGITWPNGSMGSPVRELVEP"
     gene            complement(1829..>2020)
                     /locus_tag="PLX53_06575"
     CDS             complement(1829..>2020)
                     /locus_tag="PLX53_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOY72783.1"
                     /translation="SGAKNSVPAELVRQVRSQISIPLIVGGGLRSARQVDAVFAAGAD
                     MVVVGNSVENNPAILEELG"
BASE COUNT          575 a          493 c          472 g          480 t
ORIGIN      
        1 aaatatcttt atggtttggg taagagtaaa tgacacaata attgttagaa aaatagttca
       61 gattcaacaa aaataagttg ctttgtaccg gcattcccaa gcaacttggg gaaaaaacca
      121 ttaccaattt tttagaagtt ccataaacca tgcatttaaa gagacttagc ttgttaatcc
      181 tgttcactgc aggacttagg ttatccttat gggcacagcc caaccgtcaa attccatcag
      241 aaaaaccacg tttgattgtc gaaattgtag ctagccagat gcggttcgac tacctgacaa
      301 ggtactggga taagttttcc gatggtggat ttaaaatgct agttaacgat ggtgccagct
      361 gcaaaaatgc aaggtacagc taccttttaa cccaagccta cccaggaatt gcaacaatct
      421 ccactggcgc caacccatca attcatggca taatatccga cagctggttc tcgagagtta
      481 acggcgaagt ggttgatgct gttgccgacg aaaaaacaac cactacgggt ggcagcttcg
      541 ccagcggtag ggcctcggca aaaaatcttg ttaccagcac tctgggtgat gaaatttgcc
      601 taaacaaccc ccaatcgaag gtggtgagca ttgctcttga tgccggttcg gccatactca
      661 tggccggaca tagcgcgagt gggatttact ggctcgacac cgaaaagggt ggatgggttt
      721 ccagcagcta ctttagcgac agcctacccg catgggtgga tacccttaac gctaaatcgc
      781 ttgcaaagct ttatgccgaa aaggagtgga aagcgataaa ccccatacag gcttatgaag
      841 aaaccgatac actcaccgcc aggactccag agggaaagaa aagggtatcg gataaaatta
      901 agctactgat tgacggtata ataggaaaat cggaacctgc taccagctac aaaaagctgc
      961 ttgaaacccc atttggtaac tcgcttacca aggatatggc tatagcctcc atcttaggtg
     1021 aaagccttgg tagcgatgaa aacaccgact tactctgcat cacctttagc gccaaccggt
     1081 acataacccg aaagtttggc ccacattcca ttgaaatgga ggatacctac ctgaggcttg
     1141 accgagaact ggcacacttc ctccaattcc tggcaaccca ggtcggaaag gagaatgtgc
     1201 tggtggtgct cacctccgac caaggggttt cgtcaactcc ggaatttctg gaaaagtcaa
     1261 agattccagg tggatacttc gatcctaaaa aagccatggt gctgctgggt agctacctga
     1321 atgccctata cggacagggc agctggataa caggctacca cgaaaaacag atttacctca
     1381 accgaaggtt gatagaggat tccaacataa atcttgccga aatgcagcaa aaggttgccg
     1441 attttatgct tgagttctcc ggtgtggcca acagtgcaac cgctcatacc ctccaatcag
     1501 caaattttgc aaacgggata atggtgaaat ttcagaatag ctacaaccaa cgccgttcgg
     1561 gtgatgttat tattaacctg gagccgggat gggttgaacg gaatgggtcc attacctccg
     1621 gcaactcggc ctacgactac gatacccatg tacccctaat ctggtatggc tggaaggtta
     1681 aacgtcgcgt tattacgtca ccggtaaaca tttatgacat agctcctacc ctttcgagcc
     1741 tgctggggat aacctggcct aacggttcga tgggttcccc tgtaagagag ctggttgaac
     1801 cctaagttgt gcccttcagt aagcattgct agcccaactc ttcgagtatg gcagggttgt
     1861 tttcaacgct gttgccaact actaccatgt cggcaccggc tgcaaaaacc gcgtcaacct
     1921 gcctggcgct gcgaaggccc cctccaacga ttagaggaat ggaaatttgg cttcgcacct
     1981 gccttacaag ctcagccgga acggagtttt tagccccact
//
LOCUS       DAOOQX010000382         2014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_69990_length_2014_cov_3.294028, whole genome shotgun sequence.
ACCESSION   DAOOQX010000382 DAOOQX010000000
VERSION     DAOOQX010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69990_length_2014_cov_3.294028"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..968)
                     /locus_tag="PLX53_06580"
     CDS             complement(165..968)
                     /locus_tag="PLX53_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOY72784.1"
                     /translation="MIATKGLVFDIRHFSVHDGPGIRTAVFLKGCPLRCMWCHNPESQ
                     SLLPENMKRSRKVGEKSFDFDEMVGREMTPNEVVAELQKDIPIYDESGGGATFSGGEP
                     MIQFDFLYQVLEECRRMEIKTAIDTCGHAPLEQFRAVEPLTNLFLYDLKLIDSTQHKE
                     FTGVDNSLILQNLEMLLSRKARIHIRIPLIPGITDTDENLLGIKDFLLSYSGAERIDL
                     LPYHDIARSKYVRLGRDNPFKLSGAYDSQKLSRIQDLFIDLNIPVTVGG"
     gene            complement(1397..1606)
                     /locus_tag="PLX53_06585"
     CDS             complement(1397..1606)
                     /locus_tag="PLX53_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72785.1"
                     /translation="MGLDIKLPIGLMFTIFGIILTVYGIFTNSDPQMYQKSFLVNINL
                     WSGIGMLIFGIFMLALTKKKKSEKK"
     gene            complement(1627..>2014)
                     /locus_tag="PLX53_06590"
     CDS             complement(1627..>2014)
                     /locus_tag="PLX53_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72786.1"
                     /translation="MFWKRSTGHAAFTGLLAGFLIALLHHGLTVPAGATTLVKGGWIA
                     TLHVYPVEMAQNFWTAIFAFTTSALVTVVISLLTRRTKSNDELVGLVYSLTPRMKEDH
                     VVWYKRSSTLAVVVLLIATILTILFW"
BASE COUNT          586 a          436 c          435 g          557 t
ORIGIN      
        1 ggtggggcag ccctccacct gctgcgaaga gtagtatttt gttatcagca aagctcgttc
       61 ggccgacagg gtattcaccg ctttaaggct ttgctccctt agtgtcttta ttctttggtt
      121 catacttgta acatttaact atttaaaata agtcaatgga caaactagcc tccaacagta
      181 accgggatgt ttaaatcgat gaacaaatcc tgtatccggc ttagcttttg cgaatcgtag
      241 gcgcccgaaa gcttaaatgg gttatccctg ccaagcctaa cgtacttgct gcgcgcaatg
      301 tcgtggtagg gtagcaggtc aatacgctcc gcaccgctat aggagaggag gaagtcttta
      361 atacccaaca ggttttcgtc ggtatcggta atgccaggaa ttaggggaat acgaatatga
      421 attctggcct tcctggataa aagcatttcg agattttgta aaattagcga attgtcaact
      481 cctgtaaatt ccttatgctg ggttgaatca ataagcttta ggtcgtagag gaacaggttg
      541 gtcaacggtt caaccgctct gaattgttcc aatggagcat gtccacaggt atcaatcgct
      601 gttttaattt ccatcctgcg gcactcttcc aaaacctggt agagaaaatc gaactgaatc
      661 attggctcgc ctcccgaaaa agtagctcct ccaccagatt catcataaat aggaatatct
      721 ttttgcagct cggcaacaac ctcattgggg gtcatctcgc gtcccaccat ttcgtcgaag
      781 tcaaagctct tttcacccac cttacggctt cgcttcatgt tctcgggtag taggctctgg
      841 ctctcggggt tgtggcacca catgcagcgg agcggacaac ccttaaggaa tacggctgtt
      901 cttatgcccg gaccgtcgtg taccgaaaaa tggcgaatgt cgaacaccaa ccctttagta
      961 gcgatcatgt caaaatatta tgttagaatt gtgatactat aaagaacaat acagaggata
     1021 acactatcct ttagggagaa tgaatatggg attagaaaat tttcaacaaa tccagcaggc
     1081 aaagaggcct ttgccattat tatggtagag ggtggaggta ttggatttgt tgtacatgat
     1141 accgcaactt ctaaacctat ttttacaaaa atacgctatt tcaggatacc tgtcaatgat
     1201 atttgtcatt tattaaacat ttagcttgaa gcaatttaac ccaccggtta atggttgcca
     1261 atacaaattt tagggcataa gtaagccacc ttagtaatgc caggcatgtt atggtagctt
     1321 attaaggtaa gacccaaaaa aagaagaccg ctggtgctag accaacagcc tcctttgaat
     1381 tagctaaaga tgtccgctac tttttttcgg attttttctt tttagtgagc gccagcatga
     1441 aaatgccaaa aattagcatt ccaatgcccg accataggtt gatatttacc agaaaggatt
     1501 tttggtacat ctggggatca gaattcgtga atattccata cacggtaaga attattccaa
     1561 aaatggtgaa catcagccca ataggaagtt ttatatctag tcccatgaca cttattcttt
     1621 aaatgattac cagaacaaaa tagtaagaat agttgcaatc agcaaaacta caacggcaag
     1681 agttgacgag cgtttgtacc ataccacgtg atcctccttc attcttggag tgagggagta
     1741 aactagtcct accagctcat cgttcgattt ggttcttctg gttagaagcg aaattacaac
     1801 ggtaaccaga gcgctagtgg taaaggcaaa aatagcagtc cagaaattct gagccatttc
     1861 taccggatag acgtgtaatg tagctatcca gccaccttta accagggttg ttgcaccggc
     1921 aggcacggtt aatccatgat gcagcagggc tatcagaaaa cctgcaagca accccgtgaa
     1981 ggcagcatgg ccagttgacc tcttccagaa cata
//
LOCUS       DAOOQX010000383         2014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_69998_length_2014_cov_2.625830, whole genome shotgun sequence.
ACCESSION   DAOOQX010000383 DAOOQX010000000
VERSION     DAOOQX010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69998_length_2014_cov_2.625830"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..179
                     /locus_tag="PLX53_06595"
     CDS             9..179
                     /locus_tag="PLX53_06595"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOY72787.1"
                     /translation="MPERCRQAFILSRRDGLKYKEIAAKLSVSEKTVEADMTKALMAL
                     RQNLSRLGFNTH"
     gene            228..1235
                     /locus_tag="PLX53_06600"
     CDS             228..1235
                     /locus_tag="PLX53_06600"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOY72788.1"
                     /translation="MINSTPNTQDYKKISRYLCDDMLFPERLIFLALVACKPRLRKNF
                     KAARMDMAMADKLISAGSYNPNVAWEKLHDRLENESLIPDEIRNTVTFYKGAYLKYAA
                     IAALAILLIGIPGYFLFSPSRIVVENGRDGSTMITSLPDGTSVYLAENSTLSYPKHFK
                     GKLRSVTLNGEAFFEVAPNPHKPFIVNTKAATVRVLGTTFNIKSTDSNNFELCVVEGK
                     VKVSSLGGDVLATAGERVYVRNSLLQKSISAGQPTVKHRMTRLQFQDERMEDIVGIIN
                     RTYGSNIELMGNNLRNRRISVTFENELSSIVSILSASFNLQIETEPDGTIVLSEKSVS
                     P"
     gene            1195..>2014
                     /locus_tag="PLX53_06605"
     CDS             1195..>2014
                     /locus_tag="PLX53_06605"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY72789.1"
                     /translation="MAPLFSAKNQLARKANRLFRAIAYRVALLILFAGIGINGHADEA
                     DTLLTKPITISLPAENLYNALNHIGERAGCFFIYDSRDVRGSKRIKPVTWVNKPMYEL
                     LNLLVADSLVSYRVIDRHILIYRKSKDAHSGDSTRRESYLQVGGRLYDGETRQVITFA
                     TVTLNEIGLGTITNNDGAFTMRIPSSFIHASVVISHLGYQSQTIPIELLLGKKYTIYL
                     QPRYIPIQEVIIRNIDARGIVTAMVENRYKNYASNDVYLTGFYREGVSRNNQYLG"
BASE COUNT          594 a          440 c          473 g          507 t
ORIGIN      
        1 ttcaacagct gcccgaaaga tgccgtcagg catttatact tagccgtagg gatgggctta
       61 aatacaagga aattgcagca aaactttcgg tttcggaaaa aacggttgag gctgatatga
      121 caaaagcgct gatggctctc aggcagaacc tgtcaagatt gggttttaat acccattaaa
      181 ggaggcaaag gttttcaaaa caactatttt tatttttata gataacgatg atcaatagca
      241 caccaaatac ccaggattac aaaaaaatat cccgctacct atgcgacgac atgctttttc
      301 ccgagaggtt gatttttctt gccctggtag cctgcaagcc tcggttaagg aagaatttta
      361 aggctgcaag gatggacatg gcgatggcgg ataagcttat tagtgcagga tcatataacc
      421 ccaacgtggc gtgggaaaaa ttgcacgata ggttggaaaa cgaaagcctt atccctgatg
      481 aaataaggaa tacagtgact ttctacaagg gagcatactt gaaatatgca gccattgctg
      541 ctttggccat attactcatt ggaatacccg gctactttct tttttcgccc tcaagaattg
      601 tggttgaaaa tgggagagat ggaagcacaa tgattaccag ccttcccgat ggaactagcg
      661 tttacctggc cgaaaactcc accctgagct accccaagca ctttaaaggc aaattacgaa
      721 gcgttaccct taatggtgag gcattttttg aggttgctcc aaacccccat aaacccttta
      781 ttgtgaacac gaaggctgcc actgtcaggg ttttgggaac caccttcaac atcaaatcaa
      841 ctgatagcaa taattttgaa ctttgtgtgg tggagggaaa ggtaaaagtg agcagcttgg
      901 gcggtgacgt gctggctaca gccggtgaac gggtttatgt gaggaatagc ttgcttcaaa
      961 aatccatctc tgcaggacag ccaaccgtaa agcatagaat gacacgactt cagtttcagg
     1021 atgaaaggat ggaggatatt gtaggaataa tcaatagaac atacgggtca aacattgagt
     1081 taatgggcaa caatctgaga aataggagaa tttctgttac atttgaaaat gagcttagca
     1141 gcattgtctc cattctttcg gcctccttta acctgcaaat agaaacagaa cccgatggca
     1201 ccattgttct cagcgaaaaa tcagttagcc cgtaaggcaa acaggctgtt cagggcaatc
     1261 gcatatcgag ttgcgcttct aatccttttt gcaggtatcg ggatcaacgg ccatgctgat
     1321 gaagccgata cgctgctgac caagccaata accatatcgc ttccggccga aaatctttac
     1381 aatgccctga accacattgg cgaaagggca ggctgttttt tcatttacga cagccgcgat
     1441 gtgcggggaa gtaagcgtat caagccggta acctgggtaa acaaacctat gtatgagctg
     1501 ctgaacctcc tggttgccga ttcgctggta agctacaggg taattgacag gcatattctg
     1561 atttacagga aatcaaaaga cgctcattcc ggcgattcaa cccggcgcga aagctacctg
     1621 caggtgggtg gaaggctgta cgacggggaa acccgccagg taattacgtt tgcaactgtt
     1681 acgctaaacg aaattggact tggaaccata accaacaacg atggggcttt caccatgaga
     1741 attccctcct cgtttattca tgccagcgtg gttatatcac atttaggcta ccagagccag
     1801 accattccta ttgagctgct gcttgggaag aaatatacca tttaccttca accccgatac
     1861 attcccattc aggaggtaat tatcaggaat attgatgctc gtggaattgt gacggccatg
     1921 gttgaaaacc ggtacaagaa ctatgcctca aacgatgttt accttaccgg attttacagg
     1981 gaaggggtta gccgcaataa ccagtacctc ggct
//
LOCUS       DAOOQX010000384         2012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_70118_length_2012_cov_2.476239, whole genome shotgun sequence.
ACCESSION   DAOOQX010000384 DAOOQX010000000
VERSION     DAOOQX010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70118_length_2012_cov_2.476239"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="PLX53_06610"
     CDS             complement(<1..136)
                     /locus_tag="PLX53_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOY72790.1"
                     /translation="MNPIEKIELDGVKNIVVVASGKGGVGKSTVSSNLAISLARNGYK
                     T"
     gene            complement(142..1497)
                     /locus_tag="PLX53_06615"
     CDS             complement(142..1497)
                     /locus_tag="PLX53_06615"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY72791.1"
                     /translation="MKRTDVLVIGGSAAGMVTATTGKAHWPEKSFTLVKKQKEVMVPC
                     GIPYIFGTLESSEKNIMPVDSMMEKIGVKSLVDEATDIDTKGKVVSFASGETIQYEKL
                     VIATGSNPVKPKWLAGADLENVFVIPKDRVYLDEMKEKLGSVKNIAVIGAGFIGVELS
                     DELVKCGLNVTLIEKLPHILNLAFDVELSSKIHEMLTARGVKVITGVGISKVHGTGKV
                     QSIELEDGRKMEMDAVVLSMGYRPNTELAKKAGIFIDEDNFIAVDEYMRTHTPDVFAV
                     GDCAQKRDFVTRRRVATMLASTACAEARIAGMNLFNLSVVKTFSGTIAMYSTAIGETG
                     FGTAGVTEQRAQQEGLSTLTGIFEGVNRHPGNLPGTHKQIVKLVAASNSGVIVGGEVI
                     GGLEAGELTNVIGLAIQNRMSVNSFLTMQIGTHPCLTASPAAYPLIKAAEQIAGKMIK
                     K"
     gene            complement(1515..>2012)
                     /gene="cobO"
                     /locus_tag="PLX53_06620"
     CDS             complement(1515..>2012)
                     /gene="cobO"
                     /locus_tag="PLX53_06620"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOY72792.1"
                     /translation="HLYTGNGKGKTTAAIGLAVRAAGANKTIFIGQFVKGMHYAELVS
                     LQRIPQIKVKQFGLDCFIVNNPTQKDVDAARAGFDEISEIVLNNSYNMVILDEICIAI
                     YYRLIGVNEVIDVLGKKPREMEVVLTGRYAPQELIDIADLVTEMKEIKHYYNQGIEAR
                     KGIEY"
BASE COUNT          491 a          484 c          417 g          620 t
ORIGIN      
        1 ccgttttgta accgtttcgg gcaaggctta tggccaggtt cgacgatacg gttgattttc
       61 caacaccccc tttccccgag gcaacaacca cgatgttctt tacgccatct agctcaattt
      121 tttcgatagg attcataagg gctatttttt aatcatttta ccagctattt gttctgctgc
      181 tttaataagt ggataggcgg ctggcgatgc ggttaggcat gggtgagttc caatttgcat
      241 ggttaggaac gagttaaccg acatgcggtt ttggattgcc aatccaataa catttgtaag
      301 ctcgccagct tcaagaccac caataacctc gccacctaca attactccag aatttgatgc
      361 ggcaaccagc ttaacaattt gcttatgcgt tccaggaaga ttgccggggt gcctgttaac
      421 tccttcaaaa attccggtta gagtagataa tccttcttgt tgagcgcgtt gttctgtaac
      481 accagctgtt ccaaaccctg tttcgcctat tgcagtggaa tacattgcta tggtaccgct
      541 gaaagttttt actacggata ggttgaatag gttcatcccg gcaatacgtg cttcggcgca
      601 agcggtagat gccagcatgg ttgccacacg gcggcgggta acaaaatcac gcttttgagc
      661 gcagtcccct actgcaaata catcaggagt gtgagtgcgc atatactcat caacagcaat
      721 gaagttgtcc tcatcaataa aaattccggc ctttttagct agttcggtat tgggcctgta
      781 acccattgat agaactacag catccatctc cattttccgt ccatcctcta gctcaatgga
      841 ttgaactttg ccagtgccat gaaccttgct gatgcctact ccggtaataa ccttaactcc
      901 gcgagcggtt aacatctcat gaattttgct ggagagctcc acatcgaatg ccaggtttag
      961 tatatgcggg agtttttcga taagggttac gttaagaccg cacttcacca gctcatcgga
     1021 aagttcaacg ccaataaagc cagcaccaat aacagcaatg ttttttacgg agcccagctt
     1081 ttctttcatt tcatccaggt aaaccctgtc ctttggaatc acaaaaacat tctcaaggtc
     1141 ggcaccggca agccatttag gcttaacagg attggaaccg gttgcaataa ccagtttctc
     1201 gtactggatt gtttcgccgc tggcaaagct caccactttc cctttggtat cgatatcggt
     1261 agcttcgtca accaatgact taactccaat tttctccatc atgctatcaa cgggcatgat
     1321 gtttttctcg ctactctcca gagtaccaaa gatgtaaggg attccgcagg gaaccataac
     1381 ttctttctgt ttcttcacta aggtgaaact tttttcaggc caatgggctt taccggttgt
     1441 tgcggttacc attccggcag cgctacctcc aattactaat acatctgttc gtttcatact
     1501 ttattttttt gtggttagta ttcaataccc tttcgggctt caattccttg gttgtagtaa
     1561 tgttttattt ccttcatttc ggttacaaga tcggcaatgt caataagttc ttggggcgca
     1621 taccttccgg taagaacaac ctccatttcg cgtggttttt tgccaagcac gtctataacc
     1681 tcgttaacac ctattagcct gtagtatatg gcgatgcaaa tttcgtcaag gatcaccatg
     1741 ttgtagctat tgttcagcac tatttccgaa atttcatcga accctgctct tgcagcatca
     1801 acgtcttttt gcgtggggtt gtttacaata aagcagtcga gtccaaattg cttcacttta
     1861 atttggggga tgcgttggag cgatactagc tcggcataat gcatgccttt aacgaattgg
     1921 ccaataaata ttgttttgtt agcaccagcg gctctaacgg caagcccaat ggctgcagtt
     1981 gtttttccct ttccatttcc cgtataaaga tg
//
LOCUS       DAOOQX010000385         2001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_70620_length_2001_cov_3.534943, whole genome shotgun sequence.
ACCESSION   DAOOQX010000385 DAOOQX010000000
VERSION     DAOOQX010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70620_length_2001_cov_3.534943"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="PLX53_06625"
     CDS             <1..1161
                     /locus_tag="PLX53_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOY72793.1"
                     /translation="EYRGDGIGSVLLDEVEKELGIGVRPLSWPIGQGPTFKGVYNLFE
                     QKLFLFSPDFKQKVADEVVEITDINSPELDKYIKPYTAKLREELEIINEVYPPLDINT
                     YLSGRVAPVFFGSALNNFGVKELLDCFLQLAPYPRPAEADIRTISPFEQKFSGFVFKI
                     FANMDPNHRNRIAFLKVCSGKFERNVPYYHVRMAKTFRFSSPNAFLADKKSIVDVAYP
                     GDIVGLPDSGNFKIGDTLTEGEIINFKGIPSFSPELFRYIENAEPLKYKQLAKGIDQL
                     TDEGVAQLFTQNSNGRKIIGTVGALQFDVIQYRLEHEYGAKCKYEPLNIFKACWIVSS
                     NKAQLDDFRIRKYSNLATDKQGREVYLAESPYALQMAQDKFTDIKFYFTSEF"
     gene            1605..>2001
                     /locus_tag="PLX53_06630"
     CDS             1605..>2001
                     /locus_tag="PLX53_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY72794.1"
                     /translation="MAKKPKLRFWQIWNLSFGFLGVQAGFALQNANVSRILSNYGADL
                     HHLSFFWLLAPIMGLIIQPWVGAASDKTWNRMGRRKPFIFGGAIVATLAMLFMPNSQW
                     AVLIIPPVAFGAIMLALMDASFNVTFQPFR"
BASE COUNT          548 a          409 c          409 g          635 t
ORIGIN      
        1 gaatatcgcg gagatggaat aggttctgtt ttgcttgatg aggtggaaaa agagctaggt
       61 attggagtta ggccccttag ttggcccatt ggacagggcc caacttttaa gggtgtttat
      121 aacctttttg agcagaaact tttcctgttt tcacccgact tcaaacaaaa ggtagctgac
      181 gaggtggttg aaattactga tatcaattca cccgagctgg ataaatatat taagccatat
      241 acagcaaagc tcagggagga actcgaaatt attaacgaag tttatcctcc gttagatatc
      301 aatacctacc tttcgggcag ggttgctccg gtttttttcg gaagcgccct caataacttt
      361 ggcgttaaag aacttctcga ttgttttttg cagctggcac cctatcctag acctgccgag
      421 gccgacatcc gaacaatttc accctttgag caaaaattca gcggcttcgt ttttaaaatt
      481 tttgccaaca tggatcccaa ccaccgcaat aggattgcct ttctaaaagt atgctcgggt
      541 aaatttgaac gtaatgttcc gtactaccat gttcgcatgg cgaaaacatt ccggttttct
      601 agccccaacg cttttttggc tgataaaaaa tcaatagttg atgtggctta ccccggtgat
      661 attgtaggct tgcccgattc aggaaatttc aagattggcg atacgcttac tgagggtgaa
      721 attattaatt tcaaaggtat ccccagcttc tctcccgagc ttttcaggta tattgagaat
      781 gctgagccat tgaagtataa gcagctggct aagggtatcg accagcttac tgacgaaggg
      841 gtggcccagc tgtttaccca aaattctaat ggtcgtaaaa ttataggtac agttggagcc
      901 ctccagtttg acgttatcca gtaccgcctg gagcatgagt acggcgcaaa gtgcaagtat
      961 gaaccgctca atattttcaa agcatgctgg attgtcagta gcaataaagc tcaacttgat
     1021 gattttcgta ttcgtaagta tagtaatctt gccaccgata agcagggtag ggaagtttac
     1081 cttgccgaat ctccctacgc cctgcaaatg gctcaggata agttcaccga tatcaaattc
     1141 tacttcactt ctgaatttta gaactgccct ttaactccaa atgccccata taaagggtgt
     1201 gattttgtgt tgttttgcta ctgaaattat gatcctacct attttcagta gaaaacagta
     1261 aaatttacta gtgcaaacag cccaaaatgc aggaataacc acccaagcaa ttcacaatac
     1321 tggtttggca ctatctattt gcctgaatat ctgataggta gctatgattt ttcttttacc
     1381 taatgatgct acttttttag atgaacttta gctttatttt cagcatggaa agctattcga
     1441 agaaaaattg aaaattttta aaaaaaaatt attccaccgt aaacgattga aatgctgttt
     1501 gcgtgttctg aatccctttg cctataaagg ttttaagttt tcattaaggt ttagttaacc
     1561 ttttttaaat tacttttggt gattatgaaa aaatttagtt tgatatggca aaaaaaccta
     1621 aactccgttt ttggcaaatt tggaatctaa gctttggatt tcttggagtt caagctggat
     1681 ttgctcttca aaacgccaat gtatcgcgca ttctttcaaa ctatggcgct gatctgcatc
     1741 atctttcgtt cttctggctt ttagctccta taatggggtt gataattcaa ccttgggttg
     1801 gggcagctag cgataaaaca tggaatcgaa tggggcgtcg taaaccattt atctttggag
     1861 gtgcgattgt tgccacacta gccatgttat tcatgcccaa ctcccaatgg gcagttctca
     1921 ttattcctcc ggtagccttt ggagctatca tgcttgccct aatggatgcc tcgttcaatg
     1981 ttacctttca gcctttccgg t
//
LOCUS       DAOOQX010000386         1999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_70728_length_1999_cov_3.092593, whole genome shotgun sequence.
ACCESSION   DAOOQX010000386 DAOOQX010000000
VERSION     DAOOQX010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70728_length_1999_cov_3.092593"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="PLX53_06635"
     CDS             <1..1227
                     /locus_tag="PLX53_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY72795.1"
                     /translation="LNNNTYFYPSFNLSAVLSDLFDFQSPTLNMVQVRAGWAQVGTDT
                     DPYQLQPVYRFDQGWHESTKLPAIYIPNDLPNPNLKPQRTNSLEVGATLKLFMSRLGI
                     DLTYYNQKTFDQIIRLPVSATTGYTSKIVNAGEIDNSGIELTLDGVPVRTRSGFEWSI
                     NLNFAWNKNEVKELAEGLKQYVLGGYWSLDVIAIPGQPVGTLYGYDFKRNENGKIVFY
                     DGLPAQGEIHALGNYTPDWTGSIANSLRYKNLSLNFLIDARFGGEIYSMTSTWGRYAG
                     VLKETLKGREGGIIGVGVMQNEAGEWVPNNVVVTAEEFNKAAYNNNIAYSSVFDASFV
                     KLREISLGYTFNHIANNLIKEISIAVVGRNLALLYARVPHIDPETAFSNSNVQGLEFG
                     QLPSPRSYGLSLNLKF"
     gene            1252..>1999
                     /locus_tag="PLX53_06640"
     CDS             1252..>1999
                     /locus_tag="PLX53_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOY72796.1"
                     /translation="MWRWFKIYRWIAITGILVGMVSCTGDFEELNRNPNAIGANADHP
                     DMLLTHAVESLGDQLFDVWLGHEIGSCWVQHMAKVQYTDEDRYVPRNDVINSVWNNLY
                     AGPGIEIQRIYEIGQRKGHANYIGVALVLKSYLFSVLTDLYGDIPYTQAFNPDSTLTP
                     QYDTQESIYLNLIQNLKYANELLNPYGDGIEGDILFHGDILKWKKFANSLRLRILLRM
                     SGHAEQEQFVKSEMSSILGNSTDNPIFTSNS"
BASE COUNT          560 a          483 c          450 g          506 t
ORIGIN      
        1 ctgaacaaca atacctactt ctacccatcg ttcaaccttt cagctgtcct atccgatctt
       61 ttcgattttc aatcacctac tttgaatatg gttcaggtca gagctgggtg ggcacaggta
      121 ggcactgata cagatcccta ccagctacaa ccggtttacc gcttcgacca gggatggcac
      181 gagtcaacca agctgcctgc tatttacatt cccaacgatc taccaaaccc caaccttaag
      241 ccccagcgta ctaactctct tgaggtaggt gctacactga aattgttcat gagcaggctt
      301 ggtatagacc ttacctacta caaccaaaag acattcgacc aaataatccg cctacccgtt
      361 agcgccacca ccggctacac atccaaaatt gttaacgcgg gtgaaataga caacagcggt
      421 attgagctta cgctcgatgg cgtgccggtg agaacacgtt ccggattcga atggagcata
      481 aacctgaact tcgcatggaa caaaaacgag gtgaaggagt tagccgaggg actcaagcag
      541 tacgttttgg gtggatactg gagcctcgac gtaattgcaa tcccaggtca accagttggc
      601 acactctatg gatacgactt taaacgtaat gaaaacggaa aaatagtatt ctacgatggg
      661 ttacctgcac agggcgaaat acatgctttg ggtaactaca cccccgactg gacaggtagc
      721 attgctaact cgctacgata caaaaattta tcactcaact ttctcattga tgcccgattt
      781 ggtggcgaga tttattccat gacttcaacc tggggtagat acgctggtgt tttaaaggaa
      841 acattaaaag ggcgtgaagg tggaattata ggggttggcg taatgcagaa cgaagcgggt
      901 gaatgggtac ccaacaatgt agtagttacg gccgaagagt tcaacaaggc tgcctacaac
      961 aacaacatcg cctactccag cgtttttgac gccagctttg tgaagctccg cgagatatcg
     1021 cttggctaca ccttcaacca cattgccaat aaccttatta aggagatttc tatagccgtt
     1081 gttggtcgaa accttgcact gctctacgcc cgagtgcccc atattgaccc ggaaaccgct
     1141 ttcagcaaca gcaatgtcca aggccttgaa tttggccagc tgccctcgcc ccgtagctat
     1201 gggttaagtt taaatttaaa gttttaatgc tgacaaataa tggatacccc tatgtggaga
     1261 tggtttaaga tatatagatg gattgccata acgggcatac ttgtaggcat ggtatcatgc
     1321 accggtgatt ttgaggagct aaacaggaat ccaaatgcga ttggtgcaaa tgctgaccac
     1381 cccgacatgc tgctgaccca tgctgtcgaa tcgctgggcg atcagctttt cgatgtgtgg
     1441 ctgggtcacg aaattggctc atgctgggtt cagcacatgg caaaggttca gtacaccgat
     1501 gaggacaggt atgttccccg caatgatgtt atcaattccg tttggaacaa cctttacgcc
     1561 gggccgggaa ttgaaattca aagaatttac gagataggcc agagaaaagg ccatgccaac
     1621 tacatcggcg ttgctctggt gctgaaatca tacctttttt ctgtactcac ggatttgtat
     1681 ggcgatatac cctataccca agcattcaac ccagatagca cccttacgcc acagtacgat
     1741 acacaggaaa gcatctacct gaacctcatt cagaacctga aatatgccaa cgagcttctt
     1801 aacccttatg gcgatggtat tgagggcgat atcctttttc atggcgatat ccttaaatgg
     1861 aagaaatttg ccaactcatt gcgtttaagg attttactcc ggatgtcggg tcatgccgaa
     1921 caggagcagt tcgtcaagtc agaaatgtcc tcaattttgg gaaattcaac cgacaatcca
     1981 atatttacct ctaattctg
//
LOCUS       DAOOQX010000387         1999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_70737_length_1999_cov_2.635802, whole genome shotgun sequence.
ACCESSION   DAOOQX010000387 DAOOQX010000000
VERSION     DAOOQX010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70737_length_1999_cov_2.635802"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PLX53_06645"
     CDS             <1..209
                     /locus_tag="PLX53_06645"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOY72797.1"
                     /translation="LKSNIKPSQLARTQDDYYKMYLKGKNFSDDEWVQIYVDNPAILQ
                     RPIVVGRYKAVLAIPPENMDVLFK"
     gene            227..1087
                     /locus_tag="PLX53_06650"
     CDS             227..1087
                     /locus_tag="PLX53_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOY72798.1"
                     /translation="MKKKFVLIFLASVLMSGFLPAQNENELSISQLENEVHRLVNAHR
                     KSIGLGELAPNSYIRKEAYQHSLNMAEGKVPFSHKGFEERFERLSGFLDITSGAENVA
                     NGYNSAQDIVDGWLLSPGHKKNIEGDFNLTGIGIAQAPNGTYFYTQIFVKSQERPKIV
                     ASDFESELLGLINEHRKGLGLSALKSNDVVKAEAVNYSGQMAAGRAPIGPPGYDNPMK
                     ALLSKMNAVAMTEIIAYNYYTAKEVFNSWMASTRQRETIEGSFDLTGIGVVQSADGKV
                     FVTQIFLLSR"
     gene            1105..>1999
                     /locus_tag="PLX53_06655"
     CDS             1105..>1999
                     /locus_tag="PLX53_06655"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOY72799.1"
                     /translation="MAVPEFYYQERFPLGEEETEYYLLTRDFVSLSQFEGKDILRVDP
                     QGLELLARTAMHDTSFMLRTGHVRQLAAILNDPEASKNDKFVALVLLRNAEIAAKGIL
                     PLCQDTGTATIVAKKGQRVWTGADDYEVLSRGIFQTYTKESLRYSQTVALDMYSEKNS
                     GTNLPAQIDIYATTGEEYEFLFIAKGGGSANKSMLFQETKALLNPEKLEAFLIEKIKN
                     LGTAACPPYHIAIVVGGTSAEETLKTVKLASAKYLDNLPVKGNEFGQAFRDLELEEKI
                     FRAANSIGLGAQFGGKYLALDV"
BASE COUNT          551 a          443 c          513 g          492 t
ORIGIN      
        1 tcctaaagtc gaatattaaa ccaagccagc tagcccgaac tcaggatgat tactacaaga
       61 tgtatttgaa ggggaaaaac ttttccgatg atgaatgggt tcaaatttat gttgataacc
      121 ccgccattct ccaacgaccc atcgtggtgg gcaggtataa agccgtgctg gctattcctc
      181 ctgaaaatat ggatgtactc tttaaataaa taaatagttt gtcctgatga agaagaaatt
      241 tgtcttgatt tttcttgcct ccgtccttat gagcggattt ttgccagctc agaacgaaaa
      301 tgagttgagc atttcacagc tggagaacga ggttcaccgc ctggtaaatg cgcatagaaa
      361 atctattggg ctgggagagc tggctcctaa tagctatatt cgaaaggagg cctaccagca
      421 ctcgttgaac atggccgaag gtaaggttcc ctttagccat aagggttttg aagagcgatt
      481 tgaacggcta agcggttttt tggacattac ttccggggcc gaaaatgttg ccaacggcta
      541 caactcggct caggacatag tcgatggctg gttgttaagc cccgggcata aaaaaaatat
      601 tgagggtgat tttaacctaa ccggaattgg gattgcccag gcgcccaacg gcacctactt
      661 ctacacccag atatttgtca aaagtcagga acgccctaaa attgttgcat cagactttga
      721 gtcagaacta ttagggctga taaatgagca tcgtaaagga ttagggctta gcgcactgaa
      781 aagcaacgat gtggttaagg ccgaagcggt gaactactcc gggcaaatgg cagccggtag
      841 agcgcctata ggtcctccgg ggtacgataa ccccatgaag gcgttgcttt caaagatgaa
      901 tgctgttgcc atgaccgaaa taattgccta taactactac actgccaagg aggtgtttaa
      961 cagctggatg gccagcacca ggcaacgaga gaccatagag ggaagcttcg accttaccgg
     1021 cattggagtt gtgcagtctg ccgatgggaa ggtttttgtt acgcagattt ttctacttag
     1081 tcgttaacat aaaattcaac caaaatggct gttcctgaat tttactacca ggaaaggttt
     1141 cctttgggag aggaggagac cgagtactac cttctaacca gggattttgt ttccctttcg
     1201 caatttgagg gtaaggatat tctaagggtt gacccgcagg gcctggagct gttggccaga
     1261 acagccatgc acgacacatc gttcatgctt cgcaccgggc acgtcaggca gctggcagct
     1321 attcttaacg accccgaggc tagtaagaat gacaagtttg ttgcccttgt tctgcttagg
     1381 aatgccgaaa tagccgctaa gggcatactt cccttgtgtc aggatacggg aactgcaaca
     1441 attgtggcta aaaaggggca gcgggtatgg acaggtgccg acgattacga agtcctttca
     1501 aggggaattt tccaaaccta taccaaggag agtctgcgct actcgcaaac agttgcgctg
     1561 gatatgtact ccgagaaaaa ctccgggaca aatcttccag cacagattga catctatgcc
     1621 accactggcg aggagtacga attcctgttc atcgccaagg gcggaggttc agccaataag
     1681 agcatgctat ttcaggaaac aaaggccttg cttaaccccg agaagctgga ggcttttctt
     1741 attgagaaaa taaagaactt gggaaccgct gcctgcccac cctaccatat tgccattgta
     1801 gttggcggca catcggccga ggaaacccta aaaacggtaa agctagcctc ggctaagtat
     1861 ctcgacaacc tgcccgttaa ggggaatgag tttgggcagg ctttcaggga tttggagctt
     1921 gaggagaaaa ttttccgtgc agccaacagt ataggacttg gtgcacagtt cgggggcaag
     1981 taccttgccc tggatgtta
//
LOCUS       DAOOQX010000388         1994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_70998_length_1994_cov_2.618876, whole genome shotgun sequence.
ACCESSION   DAOOQX010000388 DAOOQX010000000
VERSION     DAOOQX010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70998_length_1994_cov_2.618876"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLX53_06660"
     CDS             complement(<1..363)
                     /locus_tag="PLX53_06660"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOY72800.1"
                     /translation="MNNVKFLTGGQLPFCKGCGHVAVAQNTEKALQKLDFNPLDVVLV
                     TDIGCHGIVDKNFLTHNVHGLHGRSSALAAGISAGLSNPNKKVIVFTGDGGATIGMQH
                     LVGGAHLGFDMTVVVHNNM"
     gene            complement(451..1575)
                     /locus_tag="PLX53_06665"
     CDS             complement(451..1575)
                     /locus_tag="PLX53_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOY72801.1"
                     /translation="MHPGKTSLAFIDGSRLITESLVRAGADTFIGYPITPANLLYLYA
                     TKRYPTMLAAPDEITTLQWMSGFAIAGHVPVTATSFPGYALMLESINMAAMFELPMVI
                     VLVQRLGPATGTATCGAQGDLSLVHGTLSGGYTLPTFSPSNLNDCWDISAKALHTAIR
                     LRTPVVLLTSKEMVMTLQAFDWSHLAEIETLKVKTFSGSDTYIPYKVDESLVPDFMPV
                     SQNNHAVRLTASTHDTKGFLQNSTPEAIENTKRLEAKVTKHLQEYLLFDLDEQAGAQH
                     LVVSYGISAQASREAVRQLRAGGEKVSLLVAKTLLPVSRDYVDIANRYERVVVVEENL
                     TGQLRQLLFGAAGRNGLSGVNAIARMISPSEIVDEVMRKF"
     gene            complement(1562..1927)
                     /locus_tag="PLX53_06670"
                     /pseudo
     CDS             complement(1562..1927)
                     /locus_tag="PLX53_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
BASE COUNT          534 a          465 c          457 g          538 t
ORIGIN      
        1 catgttgtta tgcacaacca ccgtcatgtc gaatccaaga tgagctccgc cgactaaatg
       61 ctgcatcccg atagttgcac ctccatcgcc cgtaaaaact atgaccttct tattcgggtt
      121 acttaatcct gccgatattc ctgctgctaa ggccgaagag cgtccatgaa ggccgtgcac
      181 gttgtgggtg agaaaattct tatcaacaat gccatggcaa ccaatatcgg taaccagaac
      241 tacgtcaagg gggttaaaat ctagcttttg taaggccttt tcggtgttct gggcaacagc
      301 aacatgtccg cagcccttac aaaagggtag ctgtcctccg gttaaaaatt ttacgttgtt
      361 cattctaaaa atcgttatga tttaaatata accgattcat tctagtattt ccgaaattca
      421 aatattaggg tttgcataag taaataattt ctaaaacttt ctcattacct cgtcaacaat
      481 ttcggaaggg cttatcatac gggcaatagc attaacaccg cttaacccat tacgaccagc
      541 agcgccaaaa agcagctggc gcagctgtcc ggtaaggttt tcctcaacca ccactacgcg
      601 ttcgtaacgg ttggcaatgt caacataatc acgtgaaacg ggcagaaggg tcttggcaac
      661 cagcagcgac accttttcgc cccctgctct tagctgccta actgcttcgc gtgaagcttg
      721 tgcagaaatt ccatacgaga caaccagatg ttgagctcct gcttgctcat caaggtcaaa
      781 taaaaggtat tcctgaaggt gcttggtaac cttagcctcc aaccgcttcg tgttttctat
      841 agcctcggga gttgaatttt gaaggaatcc ctttgtgtcg tgtgttgaag cggttaaccg
      901 cactgcatgg ttgttttggc taactggcat aaaatcggga acaagcgatt catcaacctt
      961 gtagggaatg taggtatcgc tgccactaaa cgttttaacc ttaagtgttt caatctccgc
     1021 caaatgtgac cagtcgaaag cctgaagggt catcaccatt tcctttgatg tgagtagcac
     1081 aacaggagtt ctcagcctga tggcagtatg aagggcttta gctgaaatat cccagcaatc
     1141 gtttaagttg cttggcgaga atgttggcag ggtgtaaccg ccggaaaggg taccgtgaac
     1201 cagtgaaaga tcgccctgag ccccacaagt agccgtacct gttgccggtc ctaagcgttg
     1261 aacaagaaca attaccatgg gcagctcaaa catggccgcc atgtttatag attccagcat
     1321 cagggcataa cctgggaatg aagtggctgt aactggtaca tgtccagcaa tggcaaagcc
     1381 gctcatccat tgaagtgtcg taatttcatc gggagcagca agcattgttg ggtatctctt
     1441 tgtggcgtag aggtatagca agtttgcagg agtaattggg taacctatga aggtatcggc
     1501 gccggcacga acaagcgatt cggtaataag cctcgatcca tctataaaag ccaaactggt
     1561 tttacctggg tgcatacaca acatttttat tgattaaacc ttcaatacaa tcatttgaga
     1621 aaccaagcgt ttccttaaga atttgaattg tatgctcacc gtaatatgga ggagcagcca
     1681 actctagggg cttggaattc tcattgtaag aaaaagccac cgttcttagt ccctttatcg
     1741 gggttccggc tgaagttgtt ccgctcataa ccagctcctg cgcttcgggt tggtcgaaca
     1801 cctcctgcat gttgtaaact ccacctgcag gtatccgcct tctggcgagt tcatttaaga
     1861 aaggatcacg gtctatttgg gcaactaaat cctgtaaaat tgggtaaatc tcgttgcgat
     1921 tcttgacgcg gttgaagttg gtagattact taggatcctg agccaattcg ggcttcccca
     1981 agattgagca cagc
//
LOCUS       DAOOQX010000389         1994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_70999_length_1994_cov_2.613203, whole genome shotgun sequence.
ACCESSION   DAOOQX010000389 DAOOQX010000000
VERSION     DAOOQX010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70999_length_1994_cov_2.613203"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLX53_06675"
     CDS             <1..266
                     /locus_tag="PLX53_06675"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOY72802.1"
                     /translation="KLFYMSMIVLGKKFQKKSTLTLVGEDTVHTAMAKTVGTPLAIAA
                     KMLMTGQVNDRGVVMPIKPHLYNPILNELEEYGVKFVEEERQL"
     gene            complement(619..1701)
                     /locus_tag="PLX53_06680"
     CDS             complement(619..1701)
                     /locus_tag="PLX53_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOY72803.1"
                     /translation="MKQLVILASSLLSFFTLTAQNEKETMLRSLEIADTLKVSDTLST
                     NHRKPNMVVNVEKTIKVADGDTVEETTSDTLVVDSNTGDNNVAYQGDTIIVDRNQGNT
                     KSDSTIIRLGEKTIVIVEGKDKTTVEIPEGLNKDFRNSFRRSNRLKFKGHWAGIEMGV
                     NGFMDADHSTSMSNELEWLDLKQSRSWNVNLNFLQYSMGFGTSWIGLVTGMGLEFNDY
                     HFNNPLTLKVVNGVTVADSSYLLAGYKVDKTKLSTVNLTLPLLVEFQIPTSESDRHKI
                     YISAGVIGGLRIGTHTKVVFDDGDRHKDKGRSDFNIATFRYGVTARAGYRSLRLFANY
                     YPTQLFEKNKGPEIYPFAVGLVLIPF"
     gene            complement(1774..>1994)
                     /locus_tag="PLX53_06685"
     CDS             complement(1774..>1994)
                     /locus_tag="PLX53_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72804.1"
                     /translation="GVAFREKELAPNVAPSTAKVYTLADIAGLSLKRLAGLVGISYNV
                     KTDSTNTSKHIEVDSRLIAISTIIRKNN"
BASE COUNT          509 a          429 c          474 g          582 t
ORIGIN      
        1 tgaaattatt ttatatgtca atgatagttc ttgggaagaa gtttcagaag aaatcaactc
       61 ttacgctcgt tggcgaggat accgttcata ccgccatggc caaaacggtg ggcacacccc
      121 tggccatagc tgccaaaatg ctaatgaccg ggcaggtgaa tgacaggggt gtggtgatgc
      181 ccattaaacc ccacctctac aaccccattc tgaatgagct tgaggagtac ggggtaaagt
      241 tcgtggagga ggagcggcag ctttaagtta gaaggggagg ttagttgaat tcttacgcat
      301 ctgcaaggag ctgcatttag cctgttcatg ataatgcctt attgaaagag acagtttatt
      361 caacccaatc aacctaatca tgaagttgac gtttaccgga ttaaatgctg gaatggaaaa
      421 acagaagcat ctgctgtatt ttttcagcct tacttttcct gtaagtaaga tgtaatggga
      481 aaagggagga agagtttgag gtggtaaaag gaaagcggcc ggggcaaagc cccggccgca
      541 gccactttag cccaaccttt atacgctgtc cgaaacgttt ggtattacga tgggcattct
      601 atattctatc agcttacact agaatggtat aagcaccagt cctaccgcaa acgggtaaat
      661 ttccgggcct ttgttctttt caaaaagttg ggttgggtag tagttggcaa aaagccgtaa
      721 actcctgtat cctgctctgg cagtaacgcc gtaacggaag gttgcaatgt tgaagtcgct
      781 acgcccctta tccttatggc gatcgccatc gtcgaaaact accttggtgt gggtgccaat
      841 cctcaaccca ccaattactc ccgccgaaat ataaattttg tggcgatcgg attcgctagt
      901 gggtatctga aactctacta gcaaaggaag ggtcaggtta actgtgctta gttttgtttt
      961 atcaaccttg tatccggcaa gcaggtagga gctgtcggca accgttactc cgtttacaac
     1021 ctttagagtc aaggggttat taaaatggta gtcgttaaat tcaagcccca taccggttac
     1081 caatccaatc cagctggttc caaaacccat gctgtactgt agaaaattta ggtttacgtt
     1141 ccacgaacgg ctttgcttca ggtcgagcca ttcaagctcg ttgctcatcg aggtggagtg
     1201 gtcggcatcc ataaagccat ttacacccat ttcaattcca gcccaatgac ccttaaattt
     1261 tagtcggttg ctacgcctga acgagttcct aaaatccttg ttcagaccct cggggatttc
     1321 aacggtagtt ttatccttac cctcaacaat cacgatggtt ttttcgccaa gcctgattat
     1381 ggtagagtcc gatttcgtgt tgccttggtt tctgtccact attatcgtgt cgccttggta
     1441 agcaacatta ttgtccccgg tattgctatc aacaacaagt gtgtcggagg tagtctcctc
     1501 aaccgtatcg ccatcagcta ctttaatggt tttctccaca ttaaccacca tgttgggttt
     1561 cctatgattt gtgctgaggg tatcagagac ttttaaagtg tcagcgattt cgaggctgcg
     1621 gagcatggtt tccttttcgt tttgtgcggt tagggtgaaa aagctcagca gcgatgatgc
     1681 aagaattact agctgtttca ttttagttga agttagaagg ttgattaaaa ttttctgtag
     1741 gacggtagga taggtcgttt tgttacagca ccgctagtta ttttttcgaa ttatggtgga
     1801 gatggctatt agcctggagt caacctcaat atgcttggag gtgttggtgc tgtcggtttt
     1861 tacgttatag ctgatgccaa ccaaccctgc aagacgtttt aaactcaggc cagcaatatc
     1921 ggccagcgtg taaactttgg cagttgatgg ggctacatta ggtgctaatt ctttttcccg
     1981 aaaagcaaca cctt
//
LOCUS       DAOOQX010000390         1992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_71073_length_1992_cov_2.502839, whole genome shotgun sequence.
ACCESSION   DAOOQX010000390 DAOOQX010000000
VERSION     DAOOQX010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71073_length_1992_cov_2.502839"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            357..1160
                     /locus_tag="PLX53_06690"
     CDS             357..1160
                     /locus_tag="PLX53_06690"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOY72805.1"
                     /translation="MTKADSFNRDVVFIGAGNLATSLSVAFFNAGWRVVQVYSRTYQS
                     AATLANRLNAEPTTTLNSLIPDAQLYVVALPDSVIQTVAEQLPSLKGFLVHTSGSTSI
                     EVLSVADVLGYGVFYPLQTFSKARLVEFKNVTVCIEGNSQATLNALSSIAQAITSSVV
                     ALDSEKRRWLHLAAVFASNFTNHMLAAAYRIAEQNGVDFKIFEPLVRETVSKAFLANP
                     KDFQTGPAVRFDQQTMEQHLSMLGFSDLELQNIYKELSLSIQNFAQSKK"
     gene            1166..1693
                     /locus_tag="PLX53_06695"
     CDS             1166..1693
                     /locus_tag="PLX53_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOY72806.1"
                     /translation="MRKNFKEMLALVKAFVFDVDGVFTDGTIIIHPSGELLRTSNTRD
                     GYAVGVAVGRGFPVAIISGGKSDAVGVRFRGLGVQDIYLGVADKMIALEEFRKKYDLE
                     LANLLYMGDDLPDLEVMKLVGVPTCPHDAAYEVKQVAVYISSFNGGRGCVRDVIEQVL
                     RLNGKWGESSDMYMQ"
     gene            1787..>1992
                     /locus_tag="PLX53_06700"
     CDS             1787..>1992
                     /locus_tag="PLX53_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOY72807.1"
                     /translation="MKFFQLIRYKNLLIIIATMILMRYFIIEPILLSYGLALQMPTGV
                     FIMLVMATVLIAAGGYVINDYFDTR"
BASE COUNT          495 a          413 c          484 g          600 t
ORIGIN      
        1 actgaagttg agtaggttat ggtgcttttt ttgcagttgg gcaggtagag tagcggtagc
       61 caatgacgta gctgtgaacc gggagaggga agaagctaag gaaagaggtt gagctatatt
      121 aaccatgaat ccgtcagatc ccaacgcctg tttgctaaaa gccaggcaag ttcagttaag
      181 aaccggacct gcccggacgc tgagcctgtc gcagggtaaa ctcctcggaa tgagacaagc
      241 tttcgtcaaa agccactcag acttcgattt tctttatccc ttgccttaat tcttgaatgt
      301 cctttctcca gtaaccttat tttattagct atctttatgc tttaaaatat atggtgatga
      361 ccaaggctga ttccttcaat agggatgttg tttttattgg tgctggcaat ctggccactt
      421 cactttctgt tgcctttttt aatgccggtt ggcgtgtggt tcaggtttac tcccgaacct
      481 accaaagcgc tgccaccctt gccaaccggc tcaacgcaga gccaactacc accctcaatt
      541 cgctaattcc cgatgcccag ctatacgtgg ttgcccttcc cgactcggta attcaaaccg
      601 tagccgaaca gcttccgtct cttaaaggtt tcctggtaca tacgagtggg agtacatcaa
      661 ttgaagtgct ttcggttgct gatgttctgg gatacggggt attttatccg ctgcaaacat
      721 tttcaaaggc caggctggtt gagtttaaaa atgtaacggt ttgcattgag ggaaatagtc
      781 aggcaaccct aaacgcctta agcagtatag cccaagctat tacatcatcg gttgtagctc
      841 tcgattcgga gaaacgcagg tggttgcatc ttgctgctgt ttttgcctca aattttacca
      901 accacatgct ggctgcagca tacaggattg ccgagcagaa tggagttgat tttaagattt
      961 ttgagccgct agttagggag actgtttcaa aagcttttct tgccaatcca aaggattttc
     1021 aaacggggcc tgctgtacgg ttcgaccagc aaaccatgga gcagcatctc tccatgcttg
     1081 gattttctga cttggagttg cagaatattt acaaggagct atctttgagc attcagaatt
     1141 ttgcccaaag taaaaagtaa tgcccatgcg taaaaatttt aaggagatgt tggccctggt
     1201 aaaggctttt gttttcgatg ttgatggggt ttttaccgat ggaacaataa ttattcaccc
     1261 ttcgggtgag ctgctgagaa cttcaaatac ccgcgatggc tatgctgttg gggtagcggt
     1321 tggaaggggt tttcctgtgg ctattatttc cggcggaaaa tccgatgccg ttggggttcg
     1381 tttcaggggg ctcggtgttc aggatattta ccttggggtg gccgacaaaa tgattgctct
     1441 tgaagaattt cgtaaaaagt atgacctgga gctggctaac cttctttaca tgggcgacga
     1501 cctacctgat cttgaggtaa tgaagctggt gggtgttcca acttgtcccc atgatgccgc
     1561 ctacgaggtt aagcaggttg ctgtttatat ctcctcattt aacggaggca ggggatgcgt
     1621 tcgagatgtt attgagcagg tgctaaggct caatggtaag tggggcgaaa gttccgacat
     1681 gtacatgcag taaccctact gcttttatag gtatgctgaa aatcaagtgg aataaaacaa
     1741 caaaataggc tacttttaca ttacaccagc tattttaact tgacgtatga agttttttca
     1801 actaattcgg tataagaacc tgcttatcat tatcgccaca atgatactga tgaggtactt
     1861 tattattgaa ccaattttgc taagctatgg gttggcgctt cagatgccaa ccggcgtttt
     1921 tattatgctt gtcatggcta cggtgcttat tgctgccgga ggatatgtta taaatgatta
     1981 ttttgatacc cg
//
LOCUS       DAOOQX010000391         1990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_71179_length_1990_cov_2.636693, whole genome shotgun sequence.
ACCESSION   DAOOQX010000391 DAOOQX010000000
VERSION     DAOOQX010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71179_length_1990_cov_2.636693"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..>1990
                     /locus_tag="PLX53_06705"
     CDS             85..>1990
                     /locus_tag="PLX53_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOY72808.1"
                     /translation="MESKKAFSGILSPFAIKGVELKNRVVFLPHEMYYGSEEHLPTKK
                     QRFYYEERAKGGVGLIIVPSIGVHPTGFYSCQVAAKKESIPGLREITEAVHKHGSKIF
                     AQMTHYGNQTKSVETLQATWAPSAIPDMTVREVPKEMTIDEIHTLVKAFGDSAKLLLD
                     ADFDGFEIKVAHDGILRQFLSPATNVRTDEYGGTLLKRARVVVEVLQEIRKYIGEKPL
                     GIRLCIDEFTPKGYTIDDGIEYAKIFNPYVEYINTDYGNWESMDRTLPSLNYEQGLHL
                     KAIARIKKAVSVPVIASGRIIHPEMAEGVLKDGTADLIGMARELIAEPYWVKKIEAGD
                     PDEIVECIGCNQKCIGRLLQNRAISCVVNPAAGFEEDFSNDKLFSKSGKWKKVVVVGG
                     GPSGMKAAEIAAKKGHKVVLFEKSSELGGRVKLESALPHKEELAGTSRYLKYALKKLN
                     VDVRLNKIATKEDILAENPDITVVATGANLVNGEGTYSVEDVIGNKVKGENVLVMDFD
                     ANIEGTGAVEHLANQGKTVHWVTPVFFNGQDNEVSTVLLPIYRRLGEKKVILHPMSVM
                     INFDIGKATLLNPFFNTTETLEGIDAIVMAGTKAANNSLYNEIRSSLQNVYVIGDAAA
                     PRNTGAAIADA"
BASE COUNT          598 a          353 c          518 g          521 t
ORIGIN      
        1 ggattgacaa ataacttgtt gactaggtta tagggtaaaa aaaaggattg aatgatgttt
       61 aacttaaaat aggagggcgt ttgtatggaa tcaaaaaaag cattttcagg aattctttcc
      121 ccttttgcca ttaagggtgt ggagttgaag aatcgagtgg tgtttttgcc ccatgagatg
      181 tattatggct cagaagaaca tcttccaacc aagaaacaga ggttttacta cgaggaacgt
      241 gccaaaggtg gagttggact cattattgta cctagcatag gggtgcaccc taccggtttc
      301 tattcttgcc aggttgccgc caaaaaggag agcattcccg ggctaaggga aattactgag
      361 gcggttcaca agcatggctc aaaaattttt gctcagatga cccactatgg gaatcaaact
      421 aaaagtgtgg aaaccctgca ggctacatgg gcaccttcgg caattcctga tatgacagtg
      481 agggaagttc cgaaggaaat gaccattgat gagattcata ctttagtaaa agcttttggg
      541 gattcagcta agctgctact tgatgccgat tttgacgggt ttgagattaa ggtagcacac
      601 gatggcattc tgagacagtt tctgtctccg gctaccaatg tcagaacgga tgagtatggc
      661 ggaactcttc tgaaaagagc aagagtagtg gtagaggtac tccaggaaat aagaaagtat
      721 attggggaaa agcctctggg tataaggctt tgcattgatg aatttacacc aaagggctat
      781 accattgacg acgggattga gtatgccaaa atttttaacc cctatgtcga atacattaat
      841 actgattacg gcaactggga aagcatggat cgcaccttgc catcgttgaa ctatgaacag
      901 ggattgcatc ttaaggctat agcccggata aaaaaggctg taagtgtgcc ggtgattgct
      961 agcggaagaa ttatacatcc ggaaatggcc gaaggagttc tgaaagatgg aacagccgat
     1021 ttgattggga tggcaaggga attaattgcc gagccttact gggtaaaaaa gatagaagct
     1081 ggcgatcctg acgaaattgt tgaatgtatt ggctgtaacc agaagtgtat cggccgactt
     1141 ttgcaaaatc gcgcaattag ttgtgttgtt aatcctgctg ccggttttga ggaagatttt
     1201 tctaatgata agctctttag taaatcaggt aaatggaaaa aggttgttgt tgtgggcggg
     1261 gggccttccg gaatgaaagc cgctgaaatt gcagcaaaaa aaggccacaa ggttgtcctt
     1321 ttcgagaaaa gcagcgagct gggcggtagg gtaaagttgg aaagcgccct tcctcataaa
     1381 gaggagttgg ctggtacttc gcgatatctt aaatatgcgt tgaagaagtt gaacgttgat
     1441 gtaaggctca acaaaattgc taccaaagag gatatacttg ctgaaaatcc agatattact
     1501 gttgttgcta ccggagctaa tttggtaaat ggagagggaa cctattctgt ggaggatgtc
     1561 attggcaaca aggtaaaggg tgaaaacgtt cttgttatgg attttgatgc caatatcgag
     1621 ggtacaggtg ctgttgaaca cctggccaac cagggtaaaa cagttcattg ggtaacacct
     1681 gtattcttca acggacagga caacgaggtt tctacggtcc tgcttccaat ttatcgtagg
     1741 ctgggtgaaa aaaaggtgat tctacacccc atgtcggtaa tgataaattt tgatatcggt
     1801 aaggcaaccc tcttgaaccc attttttaac acaacagaaa cgcttgaggg tattgatgcc
     1861 attgtaatgg ccggaacaaa ggcagctaat aattcgcttt acaacgaaat acgaagcagc
     1921 ctacagaatg tatatgttat cggcgatgct gctgctccgc gaaacacagg ggcggcaata
     1981 gccgatgcca
//
LOCUS       DAOOQX010000392         1988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_71247_length_1988_cov_4.240041, whole genome shotgun sequence.
ACCESSION   DAOOQX010000392 DAOOQX010000000
VERSION     DAOOQX010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71247_length_1988_cov_4.240041"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..937
                     /locus_tag="PLX53_06710"
     CDS             14..937
                     /locus_tag="PLX53_06710"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HOY72809.1"
                     /translation="MNKLTTTIIMVSFMITAFSGESKSQVGGMLKDRLKKAAVKSVLS
                     NQDEGQSDTISEKQQNQQAENPTETYMQAKMMGLMGKGNVKYDLSYTFTSSMKMDFEV
                     LDSAQNEPFTGTYTLYFEKDGKNFAMEVETAGKKDEPASKSLFIYDFRNLALLILSEQ
                     DGQKTGMAMAIPPDSSKVEPSQVAPTTDTELPGNEVSTGDVTFRATGRTKSILGYSCK
                     EYLMENPEGKVELWVTNDVRYDYSDAYNRMQGFQMMSNVNSSATNGTVLEMHFKDSGS
                     PAHSDFYVREINKDKSQTFNLAGYTIMSFGQ"
     gene            1786..1977
                     /locus_tag="PLX53_06715"
     CDS             1786..1977
                     /locus_tag="PLX53_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOY72810.1"
                     /translation="MFAGGKSSRMGKRLLPVNGKPLIQHIAEQLVDFLDEAIIGANDT
                     EKYGFLKLRVDPDIWRKAL"
BASE COUNT          581 a          386 c          460 g          561 t
ORIGIN      
        1 taaataacat accatgaata agttaactac aaccattata atggtttcat ttatgataac
       61 cgcttttagc ggggaatcga agtcgcaggt ggggggaatg cttaaggata ggcttaaaaa
      121 ggctgctgta aagagtgtgc tatccaatca ggatgagggt caaagcgata ccattagtga
      181 aaaacagcaa aaccagcagg ccgaaaaccc aaccgaaacc tacatgcagg caaagatgat
      241 ggggttgatg ggaaagggaa acgtgaagta cgatttatcc tacaccttca cctcgagcat
      301 gaagatggat tttgaggtgc tagattcagc gcagaatgag ccttttacag gaacctacac
      361 cctttacttt gaaaaggatg gtaaaaattt tgccatggag gttgagactg ccggcaaaaa
      421 ggatgagcca gcctcaaaat cgctattcat ctatgatttc cgcaatttgg ccctgctgat
      481 tcttagcgag caggacgggc aaaagaccgg catggccatg gctattccac ccgattcttc
      541 taaggtagaa ccttctcagg tggcgcctac cacggacact gagctacctg gcaacgaagt
      601 ttcaactggt gatgtaacct ttagggcaac cggacgaacc aaaagcatcc ttggctacag
      661 ctgtaaggag taccttatgg agaaccccga gggtaaggtt gaactttggg taacaaacga
      721 tgtgcgctat gactactccg atgcctataa ccggatgcag ggattccaaa tgatgtcgaa
      781 cgtgaactca tcagcaacca atggaacagt gctggaaatg cacttcaagg attctggttc
      841 acctgcgcat tccgactttt acgtcaggga gataaacaag gacaagagcc aaacatttaa
      901 cctggccggc tacaccatta tgagctttgg gcagtaggct ggttgaggat ttggaggcac
      961 ttaccgcatt acttcaaatg attgcggcat ttgccattta cggcagttta ttgcaggggg
     1021 agcgctgtag gaatatggag ctagatgaat atctaacctg acgcaatggt caaggttgtc
     1081 atttctaccg tttagttgtt attaccggtt ggtaatgcta gaaaggtttt ccagcttagc
     1141 tatccaacca ttaaaatggc tgcatgtttt ttttagaaag tcaatcccta tttccccaac
     1201 aattactgcc ccaacgctta ctttatcgta ttccggaata agttttatta ttcttttcga
     1261 aggtgctgtt tcggggttat cattaattag ctctggattc accttttcag cgagaagttt
     1321 ctgtaagtta cttatttctt tggagcgctc aaaatattca atttttaatt cttcaggttt
     1381 tgatagtatg agtgtttcaa attcctatag ctgaaggtag ggtataaatc tattatcttg
     1441 aatatcatct gcaaatgctt tttcgaggaa ttcgacccgt tcataggcat cattaatttt
     1501 ttttgactca ttatattttg gaaaatcttt tggttggcgc acgattctat tcctcagaat
     1561 gtttcggtag tggtggaatt aaattcgatt cgaaaagtcc ttgacacggg tctgttcatt
     1621 gtaataagaa atttaggaga aacaaggggt aaaaaagggc tgctcaaaag taattagcct
     1681 gccgataaaa ttattggctt tcaaaataac tcctgggttt ttgagccagg gcgtatattt
     1741 gttaaagacg gaacatagca tattcgtcaa aaagcaaccg caataatttt tgccggaggc
     1801 aaaagttccc gaatgggtaa gaggttgctc cctgtaaacg gaaaacctct aattcagcac
     1861 atcgcagagc agctggttga ttttctcgac gaggcaatta tcggggcaaa cgatacggaa
     1921 aagtatggct tcctaaagct gagggttgat cctgatattt ggagaaaggc actttaatgt
     1981 gaatttac
//
LOCUS       DAOOQX010000393         1984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_71480_length_1984_cov_3.227061, whole genome shotgun sequence.
ACCESSION   DAOOQX010000393 DAOOQX010000000
VERSION     DAOOQX010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71480_length_1984_cov_3.227061"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PLX53_06720"
     CDS             complement(<1..815)
                     /locus_tag="PLX53_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72811.1"
                     /translation="MRVSGIKNKIVALLLGSMVALGLQAQTSFTPINLGDRVNSEYPE
                     INPILHPDGKTLYFSRANNPENTWGGVNSQDIWYSVLGDDGTWSQAQRLPRAVNIGRY
                     NALFTILNNGKTFLINGHYSPNGKYWIERGLSTVEQVENENWGKPQSIEVKGYSNKNR
                     GKTTTIFVSPDEQKLFMAFSKKVNGKKASIYLSERKGDRRYSKPKKINFLGNNKCESM
                     EAPFLTSDGNALYYSCKINGDNNLYMSHRTDDTYRSWSEPIPLNDTINTPGWE"
     gene            complement(859..>1984)
                     /locus_tag="PLX53_06725"
     CDS             complement(859..>1984)
                     /locus_tag="PLX53_06725"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOY72812.1"
                     /translation="FDPNLLLDDLRDKNLFYMGENTDIFHIRTKILNGYYWFAIRQRM
                     ESSFFYPYDLFRMVIEGNEPYIGQTLDFSNLKIFTSLFNEYSFGMTKEYPHWIFGGRL
                     SLLQGLSNINFNPTRLSIKVEDEMYGQLANADAQLRTSGIPKDGNGDPSFDHVEEPEW
                     ITDYFTNFKNKGFALSGGATYRYDDRTRISLSFRDVGFITWKDSIETYTLNGNTNFTG
                     LDIMSDLLYGNDVEVDSIFENILDEDFHRDTIYNKYTTWLNPKFNLTASYDLARRTTV
                     GFSFSGVVNQKLYTALTLSLSQGIGRFFSIVGTASINQRSFTNLGLGLMLKPGPLQIF
                     VVADNLYAAHNPLTTTNFNIRVGVNLVFGRINQPEGLPFR"
BASE COUNT          485 a          431 c          481 g          587 t
ORIGIN      
        1 cattcccaac ccggagtgtt aattgtatcg tttagcggta tgggctccga ccagctgcgg
       61 taggtatcat cggtgcggtg gctcatgtac aggttgttat caccattaat cttgcagctg
      121 tagtacaggg cgttaccatc ggaggtaagg aaaggtgcct ccatgctttc gcacttgtta
      181 tttcctagaa aatttatttt tttaggtttg gagtaacgcc tgtcgccttt tcgctcggac
      241 aggtaaatgc tggccttttt accatttact tttttactga atgccatgaa gagtttttgc
      301 tcgtcggggc ttacgaatat agttgtggtt tttcccctat tcttgttgct ataacccttt
      361 acctcaatac tttgaggttt cccccaattt tcattctcaa cttgctcaac agtcgaaagt
      421 cccctttcaa tccagtactt tccatttgga gagtaatggc cattaatgag aaaagttttc
      481 ccgttgttca ggatggtaaa cagggcgttg tacctaccaa tattaacagc acggggtagc
      541 ctttgggcct ggctccatgt tccgtcgtct cctaaaacgg agtaccatat atcctggctg
      601 ttaacgcctc cccatgtatt ctctgggttg ttggcccgcg aaaaatacag agttttacca
      661 tcgggatgca gtatagggtt aatttcaggg tattcagaat ttaccctatc tcccaggttg
      721 ataggggtga aagaggtctg ggcctgcagg cctaacgcta ccatgcttcc aagaagcagt
      781 gctactattt tattcttaat tcctgatact ctcatgtgtg aaacggatta agtcggaaat
      841 tcatcaattt gtgttgcatt aacggaaggg caatccttcg ggttggttta ttctaccgaa
      901 tacaagattc acacctaccc taatgttgaa gtttgtagta gtcagcgggt tatgggcggc
      961 gtaaaggttg tcggcaacca caaaaatttg cagtgggccc ggcttcagca ttaggccaag
     1021 tcccagattg gtaaaagatc tctggttgat ggatgccgtg ccaacaatgc taaaaaaccg
     1081 gccaatcccc tgcgaaaggc ttagggttaa tgcggtgtag agtttctggt taactactcc
     1141 ggaaaaggaa aatccaacag ttgttctacg agccaggtcg tagctagccg taaggttaaa
     1201 cttcgggttg agccatgtgg tgtacttgtt gtatatggtg tcacggtgaa aatcctcatc
     1261 gaggatattt tcaaaaatgg agtccacctc aacatcgttg ccgtaaagga ggtcgctcat
     1321 gatatccagc ccggtgaagt tggtgtttcc gttaagggtg taggtttcaa tgctgtcctt
     1381 ccatgtgata aacccaacgt cgcggaatga aagcgaaatt cgtgtccggt cgtcatacct
     1441 gtaggtagca cccccgctaa gcgcaaatcc tttgttcttg aagttggtaa agtaatcggt
     1501 tatccactca ggttcttcaa catgatcgaa gctgggatcg ccattgccat ctttgggaat
     1561 gcccgatgtt ctcagctggg catctgcgtt ggccagctgg ccgtacattt catcttccac
     1621 cttaatgcta agccttgtgg ggttaaaatt aatgttgctt aggccttgta gcaggctaag
     1681 cctgccacca aaaatccagt gggggtactc cttcgtcatg ccaaaggagt actcgttgaa
     1741 tagggaggtg aaaatcttca ggtttgagaa gtcgagggtt tggccgatgt agggttcatt
     1801 accctcaata accatgcgga aaagatcgta tgggtagaaa aacgacgatt ccattctttg
     1861 cctaatggca aaccagtagt acccattaag aatttttgta cgaatatgga agatgtccgt
     1921 attctcaccc atgtagaata ggtttttatc cctgagatcg tcgagcagca ggttggggtc
     1981 aaag
//
LOCUS       DAOOQX010000394         1984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_71498_length_1984_cov_2.304821, whole genome shotgun sequence.
ACCESSION   DAOOQX010000394 DAOOQX010000000
VERSION     DAOOQX010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71498_length_1984_cov_2.304821"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PLX53_06730"
     CDS             complement(<1..732)
                     /locus_tag="PLX53_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72813.1"
                     /translation="MKINFLNRQQVDEARWNACMDASVNGMIYGYSWYLDIVSPGWCA
                     LADEDYHYIFPLPRLKRNFNEIIIQPLYTQQLGLFSDSHLSPEILDEFICAIPSRYRY
                     IGINLNTLNRTERYADNTSARVTYRLDLFSSYENIQKKYCQNTRRNNLKAYALGCRVE
                     RSITPAEFLGFYSKYGVPRQRMRHYVKVRQLVESLMKRDRGELVGVYTPGSQLCAVSL
                     ICKSNGKLIYLLAASSPLGRQNRAMF"
     gene            complement(722..>1984)
                     /locus_tag="PLX53_06735"
     CDS             complement(722..>1984)
                     /locus_tag="PLX53_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOY72814.1"
                     /translation="LIFNDILAIKFRITTSHEEAAAYHGPLLAYNKEPVNGAIQIVPH
                     GLLNEKGISPQNFEVLSWRNLPVFPVTSPNGDFPFDIFTASFYLVSRYEEQLPATLDE
                     HKRYRSEQSIAVKNNFLELPVIDLWAFQLLDMLQSRFNGVAGCSRKFSTMFTFDLDSA
                     YAYRHKGFVRSALGITRSLLNLRWKELQSRARVLSGAINDPNDVYAEVARKLKGFDGT
                     TWFIHAGSWGQHDKPISLLHPAMKKIVADLSKEFKLGIHPSYKSFRKDDTLSKEINRL
                     SGVVGMKITRSRQHYLRFRLPETYIELTKMGIHEEYSMGYPDRIGFRAGTCTPFTFYD
                     LASDETLNLKVYPFAVMDSTLIFTNNCSIGESIQTINGLIDVVKSVKGLFISVWHVDY
                     LSESYPGKGWWKVLDAVISRLKEAKGEN"
BASE COUNT          509 a          465 c          463 g          547 t
ORIGIN      
        1 aaacattgcc ctattttgcc tgcccaatgg tgatgaggca gcaaggaggt agattagctt
       61 gccattgctt ttacagatga gcgataccgc acatagctgg cttccggggg tgtaaactcc
      121 gaccaattca cccctatctc ttttcataag gctctcaacc agctgcctca ccttaacgta
      181 gtgcctcata cgctggcggg gaactccgta ttttgagtag aatcctagga attcagctgg
      241 tgtaatgctt ctctctactc tacaacctaa ggcgtaagct ttcaggttgt tcctgcgggt
      301 attctggcag tacttctttt gaatgttttc gtaggacgag aacaggtcga gtctgtaggt
      361 aacccgtgcg ctggtattat cggcatacct ttccgttcgg ttaagcgtgt tcaggttaat
      421 gccgatgtac ctatacctgc tgggaatggc gcaaataaac tcatcgagta tttcgggtga
      481 caggtggcta tccgaaaaca gtccaagctg ctgggtgtag agcggctgga taattatttc
      541 gttgaagttg cgcttcaggc gaggcaacgg gaaaatatag tggtagtctt cgtcagccaa
      601 agcgcaccag ccgggcgaga cgatgtcgag gtaccagctg tagccataaa tcatcccgtt
      661 taccgaggca tccatgcagg cattccagcg tgcctcgtca acctgctgcc tgtttaaaaa
      721 gttaattttc acctttagct tcctttaatc ggctgattac tgcatccaaa accttccacc
      781 atcctttgcc ggggtaggat tcggaaaggt aatcaacatg ccaaacgctg atgaacaaac
      841 cctttaccga ctttacaacg tcaatcaacc catttatagt ctgtatggat tcacctatgg
      901 agcaattgtt tgtaaaaatt aaggtggaat ccataaccgc aaatggataa actttgaggt
      961 ttagggtttc atcgcttgcc aaatcgtaaa aggtaaacgg ggtgcatgtc ccggctctaa
     1021 agcctatcct atcgggatac cccatagagt attcctcatg aatacccatt tttgtaagtt
     1081 caatgtaggt ttcgggcagt ctaaatctca ggtagtgctg ccggctgcgg gtaattttca
     1141 ttcctacaac ccccgacaac ctgttgattt ccttgctgag cgtgtcatcc ttcctgaatg
     1201 atttgtatga tggatgaatg ccaagtttga attccttcga caaatctgca acaatttttt
     1261 tcatggcagg atgtaacagg ctgatgggct tgtcgtgctg tccccaactt ccagcatgga
     1321 taaaccaggt ggtaccatca aaacctttta gcttcctggc aacttcggcg tacacatcat
     1381 ttggatcgtt aatggctccg cttaggactc ttgcccggct ttgcagctcc ttccatctga
     1441 ggtttagcag cgagcgggtg atacccaagg cagacctaac gaaccctttg tgccgatagg
     1501 cataggcgct atccaaatcg aaggtgaaca ttgtgctgaa cttacgggaa caacccgcta
     1561 caccattaaa ccttgattgc agcatatcaa gtagctgaaa tgcccacaaa tcaataacgg
     1621 ggagctccaa aaagttgttt ttaacggcaa tgctctgctc tgagcggtag cgtttatgct
     1681 catccaacgt agccggcagc tgctcctcat aacggctaac caggtagaac gaggctgtaa
     1741 aaatatcaaa tgggaaatcg ccattaggtg aggttacagg gaaaacaggt aagtttctcc
     1801 atgatagaac ttcaaaattt tggggtgata tccccttttc gttgagcaat ccatgtggaa
     1861 caatttggat agccccattt acaggctctt tattgtaggc taacagtggg ccatggtagg
     1921 ccgcagcttc ttcgtgcgag gtagtgatac gaaactttat ggccagaata tcattgaaga
     1981 ttaa
//
LOCUS       DAOOQX010000395         1980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_71687_length_1980_cov_2.569870, whole genome shotgun sequence.
ACCESSION   DAOOQX010000395 DAOOQX010000000
VERSION     DAOOQX010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71687_length_1980_cov_2.569870"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /gene="sufB"
                     /locus_tag="PLX53_06740"
     CDS             <1..1201
                     /gene="sufB"
                     /locus_tag="PLX53_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOY72815.1"
                     /translation="IFYAAPRKRNASTGGGGEIDPELKATFDKLGIPLEEQQMLSGVA
                     VDAVMDSTSVKTTFRDTLAEHGIIFCSMSEAIREHPDLVRKYLASVVPSTDNFFASLN
                     SAVFSDGSFCYIPKGVRCPMELSTYFRINAANTGQFERTLIVADDDSYVSYLEGCTAP
                     MRDENQLHAAVVEIVALENAEVKYSTVQNWYPGDKNGKGGIFNFVTKRGICKGKNSKI
                     SWTQVETGSAITWKYPSCILMGDNSYGEFYSVAVTNHHQQADTGTKMIHIGKNTRSRI
                     VSKGISSGEGSNSYRGLVKVLKGADNARNFSQCDSLLIGDRCGAHTFPYIEVDNPTAI
                     AEHEATTSKIGEDQLFYCNQRGISTEDAVGLIINGYAKEVLNKLPMEFAVEAQKLLQV
                     SLEGSVG"
     gene            1387..>1980
                     /gene="sufC"
                     /locus_tag="PLX53_06745"
     CDS             1387..>1980
                     /gene="sufC"
                     /locus_tag="PLX53_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOY72816.1"
                     /translation="MLIVNDLHARINDKEILKGINLEVKAGEVHAIMGPNGSGKSTLA
                     SVLTGREMVEVTHGQVTFNGKNLLEMPPEVRSREGIFLSFQYPVEIPGVSMVNFMKAA
                     VNEQRKYRGLEPLSATDFLKLMRERKELVEIDSALTNRSVNEGFSGGEKKKNEIFQMA
                     MLEPKLAILDETDSGLDIDALRIVANGVNKLKSPDNAV"
BASE COUNT          555 a          435 c          500 g          490 t
ORIGIN      
        1 aattttctat gctgctccgc gaaaacgaaa tgcttcaacc ggagggggcg gggaaattga
       61 ccctgagcta aaggcaactt tcgacaagct aggcattccc cttgaggagc aacaaatgct
      121 ttcaggagtt gctgttgatg cagttatgga cagcacttcg gtgaagacga catttcggga
      181 tactttagcc gagcatggca ttattttttg ttcaatgagc gaagccattc gcgagcatcc
      241 cgacttggtg cgtaaatacc tggcctctgt tgttccctcc accgacaact tttttgcctc
      301 cctcaactct gccgtcttca gcgatggttc gttttgctat attcctaagg gagtgcgttg
      361 ccccatggag ctaagcacct actttagaat aaatgctgcc aataccggac agtttgagcg
      421 caccttgata gttgccgacg acgatagcta cgtttcctac cttgaggggt gcactgcccc
      481 catgcgcgac gaaaaccagc ttcatgcagc agtggtagaa attgttgccc tggaaaatgc
      541 cgaggtgaag tattccactg ttcagaactg gtatccaggc gacaaaaacg ggaagggcgg
      601 tatattcaac tttgtaacca aacgtggcat ttgcaaggga aaaaacagca agatttcgtg
      661 gacacaggtt gaaacagggt cggccattac atggaagtat cctagctgca tacttatggg
      721 cgacaactcc tacggtgaat tttactccgt tgctgtaacc aaccaccatc agcaggccga
      781 cacgggaaca aaaatgatac acatcggcaa gaatacccgt agccgaatcg tttcaaaagg
      841 aatttcgtca ggtgagggca gcaatagcta ccgtgggctg gttaaggtgc ttaagggggc
      901 cgataatgcg cgcaacttct ctcagtgcga ctccttgcta attggtgata ggtgtggtgc
      961 gcataccttt ccctacatcg aagtggacaa cccaaccgcc atagccgagc atgaagcgac
     1021 aacttcaaaa atcggtgagg atcagctttt ttactgcaac cagcgtggaa tttcaactga
     1081 ggatgccgtt ggtctaatta ttaacggata tgcaaaggaa gtgctcaaca agctgcctat
     1141 ggagtttgcc gttgaggctc agaagctcct tcaggtgagc ctggagggca gcgtaggtta
     1201 gaaacattcg aagaaaggtg aaatggtaaa gctacttgaa tactaccggc ttaatcgccc
     1261 gtataaccta ctcgtttgga attgaagtcc tcaaatatac cggtttacat gaattttgat
     1321 gtgtaatttt atggtgggtc aacacggaaa tattaatttg gaaacatata cattaatcat
     1381 catagtatgc taatagtcaa cgatttacat gctagaataa acgataagga aatattaaag
     1441 ggtataaacc tggaggttaa agccggagaa gttcacgcca ttatgggtcc gaatggctca
     1501 ggcaaaagca ctttagcttc ggtgctaacc ggcagggaga tggttgaggt tacccatggg
     1561 caggttacct ttaatggaaa aaaccttttg gagatgccac ccgaagtgcg ttcgcgcgag
     1621 gggatttttc tcagctttca gtacccggtt gaaatacccg gcgttagcat ggtaaacttt
     1681 atgaaggctg cagttaacga gcagcgcaag tatagaggtc ttgaacccct ttcggcaacc
     1741 gattttctta agctgatgcg tgagcggaag gagctggttg aaattgactc tgccctaacc
     1801 aaccgttcgg taaacgaggg tttttccggt ggagaaaaga aaaaaaacga aatattccaa
     1861 atggccatgc tggaacccaa gctggctata cttgatgaaa ccgactcggg tttggatatc
     1921 gacgcactgc gaatagtagc caatggggtt aacaagctca aaagcccgga taatgccgta
//
LOCUS       DAOOQX010000396         1976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_71914_length_1976_cov_3.013535, whole genome shotgun sequence.
ACCESSION   DAOOQX010000396 DAOOQX010000000
VERSION     DAOOQX010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71914_length_1976_cov_3.013535"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PLX53_06750"
     CDS             <1..666
                     /locus_tag="PLX53_06750"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOY72817.1"
                     /translation="IVNAVAILVGGVLGLVLKSRLPEKYTAVFFQVIGLFTLVLGFSM
                     SIKTEKPLLMVFSLILGGVSGIILNLQQRIDTWAIALKNRFKLKGDRFAEGMVVAFLL
                     FCMGSLTVLGALEEGTGGEPKLFYVKSLMDGFSALALASTLGVGVIFSILPLLVYQGG
                     LTWIAAATAKSLSNAMVNELSAVGGVLLIALGINILGIKKLEVVNLLPSLVFIILLFY
                     LFG"
     gene            659..>1976
                     /locus_tag="PLX53_06755"
     CDS             659..>1976
                     /locus_tag="PLX53_06755"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOY72818.1"
                     /translation="MDNLIMMMHALEEQHAELAKLFRNTFSVEPLCFEPIPVSGSLRK
                     YYRLQNGNTSAVGTFSPDVDENRTFFKLTDCFTRQSLNVPQVLAISPCMRYYLQSDLG
                     STSLFDIITSPGKFPQHNLNNFLSIAVTSLVDFQVKGFNEISSSGQLQLPLFDRTSMM
                     WDMYYFKYCFLKPSGISFNEQLLESDFEWFAKELLEQKHFLFQYRDFQSRNIMVHNNQ
                     LYFIDYQGGRIGPPLYDIASFLYQARAKLDNDKRFELLSLYLDRLNCILSIDKEELKA
                     KFPAMAMFRIMQTLGAYGFRGFFEKKTHFIQSIPLAVDNFINLSRSAPGPHTAYITDL
                     LEQYRVLFQPSSCLSPSGQELTVYITSFSFKNGYPEEHPEHGGGFVFDCRCLPNPGRM
                     DRYKNLTGLDFEVIQFLDESDEAQRFIANATALVKDAVENYCQRRFS"
BASE COUNT          505 a          436 c          412 g          623 t
ORIGIN      
        1 atagttaatg ccgtggccat actcgtagga ggagtgctgg gcttggtgct gaagtcgcgg
       61 ctacctgaaa aatataccgc agtttttttt caggtgatag gcttgtttac tcttgttttg
      121 ggattttcca tgagcattaa aaccgagaaa ccactgctga tggttttcag cttaattcta
      181 ggaggtgttt cggggataat cctaaaccta cagcagcgaa ttgatacatg ggcaatagct
      241 cttaaaaaca ggtttaagct caaaggcgat aggtttgccg agggaatggt tgttgctttt
      301 ctgcttttct gcatgggctc tttgactgtt ctgggtgcac tcgaagaggg aaccggcgga
      361 gagccaaaac ttttctacgt taaaagcttg atggatggtt tttctgcctt agcgcttgca
      421 tcaaccctgg gagttggcgt tatcttttca atattaccct tgttggttta ccaaggcggt
      481 cttacatgga tagctgcagc cactgccaaa tctctatcca atgccatggt taatgaactt
      541 tctgcagttg gtggagtact gcttattgca cttggaatca atattcttgg aattaaaaag
      601 ctggaggtag tcaacctttt accttccctg gtttttatta tccttttatt ttacctgttt
      661 ggataatttg attatgatga tgcacgcttt agaagaacag catgctgagc tggcaaaact
      721 cttcagaaac acctttagcg ttgaacctct ctgcttcgaa ccaattcctg tttcaggttc
      781 ccttcgaaaa tactaccgct tacagaacgg caacacgtca gcagttggaa ctttcagccc
      841 tgatgttgac gaaaaccgca cttttttcaa gcttaccgat tgcttcacaa ggcaatccct
      901 taatgtacct caagtattgg ctatttcacc ctgtatgcgc tactacctgc aatcagattt
      961 gggctccaca tcccttttcg acattattac ttctcccggt aagtttcccc agcacaacct
     1021 taacaatttt ctatccattg cggttaccag ccttgttgac tttcaagtta aaggttttaa
     1081 tgagattagc tcttccggtc aacttcaact ccccttgttt gaccgcacct cgatgatgtg
     1141 ggatatgtac tactttaaat actgcttcct caagccctca ggaatttcat tcaacgaaca
     1201 gctactcgaa tccgattttg agtggtttgc caaggagctg ctagagcaaa agcactttct
     1261 atttcagtac cgtgatttcc agtcgcgtaa tattatggtg cacaacaacc agctgtactt
     1321 tatagactat cagggtggga gaatcgggcc accactttat gatatagcct cctttttata
     1381 ccaggcaaga gcaaagctgg ataatgataa acggttcgaa ctgctaagcc tttacttaga
     1441 caggttaaat tgcattctct ctatagataa ggaagaatta aaggctaaat ttccagcaat
     1501 ggccatgttt cgtatcatgc aaactctagg tgcttatggt tttagaggct tttttgagaa
     1561 gaaaacacat tttatccaaa gcatacccct ggctgttgac aactttatca acctctctcg
     1621 ctccgctcct gggcctcata cagcttatat taccgacctt ttagaacagt atagagtttt
     1681 atttcaaccc tcatcctgct tgtcaccatc gggtcaggag cttactgtat atattaccag
     1741 cttctctttt aaaaatggct atcctgagga gcacccggag catggaggag gctttgtgtt
     1801 cgattgcagg tgtttgccta accctggtcg aatggataga tataaaaacc ttacaggact
     1861 tgatttcgaa gtaattcaat tccttgatga gagcgatgag gctcagagat ttattgccaa
     1921 tgctactgcc ttggtaaagg atgcagttga gaattattgc caacgacgtt tctcct
//
LOCUS       DAOOQX010000397         1976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_71922_length_1976_cov_2.631442, whole genome shotgun sequence.
ACCESSION   DAOOQX010000397 DAOOQX010000000
VERSION     DAOOQX010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71922_length_1976_cov_2.631442"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="PLX53_06760"
     CDS             complement(<1..1263)
                     /locus_tag="PLX53_06760"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOY72819.1"
                     /translation="MNFTQGALFTISSIIISSGFAQARGSSDTISSRKSVEAFRIESH
                     ISINGHLDEPEWQLAGFAESFTQYEPYNGASPSQPTRVKMLYDDDALYVGAWLFDSSP
                     DSIYKELGQRDNSDNLKSDAFSVYISTYNDGVNYLQFTVSASGVQTDIKVTSDEEDRS
                     WNAVWESEVSMDNRGWYVEMKIPYSALRFSKNDTTVWGVNFLRLLKRYNEKCTWNFVD
                     KSKSGFTTQSGVLVGIAGIKPPPRLSFMPYLSAYANRYPDKSGTDYQVTGGMDLKLGL
                     SQSFTLDATLIPDFGQVRSDDRILNLSPFEVKYSEARQFFTEGTELFNKGGIFYSRRV
                     GSTPVDYDKAYESLDTNEVVSDNPSSTKLINASKLSGRTSGGLGIGVFNAVSRKTYAE
                     ITDTATGQHRKFLTQGLTNYNMLVFDQSL"
     gene            complement(1427..>1976)
                     /locus_tag="PLX53_06765"
     CDS             complement(1427..>1976)
                     /locus_tag="PLX53_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOY72820.1"
                     /translation="ISADRIIIDDFDGAYQATEHLIIQGRRKIVHFSGPQNRLIGQNR
                     LNGYLKAMRDNGVVIDERLIIHCDTFQSALIETQKLIDSGTKFDSIFTVNDFTAAGAM
                     KILIRNGIKIPQDVSVVGFGDDQTSLMVEPTLTTVNQPGFEMGKKAMEQLIRRITQTR
                     QEPPITEILKTQLIVRESSRLR"
BASE COUNT          525 a          448 c          449 g          554 t
ORIGIN      
        1 tagcgactga tcaaaaacca gcatgttgta gttggtaagc ccttgggtta aaaacttcct
       61 gtgctgcccg gtagctgtgt cggtgatttc ggcgtatgtt ttacgggata ctgcattgaa
      121 aacgccaata cccaatcctc cggatgttcg cccggaaagt ttgctggcat taatcagctt
      181 ggtgctggat ggattatcgc ttacaacctc gttggtatcg aggctttcgt aagccttatc
      241 gtaatcaacc ggagtgctgc caacccttcg ggagtaaaaa attcccccct tattgaatag
      301 ttctgtacct tcggtgaaga actgcctggc ctcgctgtac ttcacctcaa agggagaaag
      361 gttgagtatt ctatcgtcgg agcgaacctg cccaaaatcg ggtataaggg tagcatcgag
      421 agtaaaactc tggctaaggc ccaacttcaa gtccattcct ccggtaacct ggtaatcggt
      481 accactcttg tcgggatacc tgttggcgta agctgaaagg tagggcataa aagacaacct
      541 ggggggtggc ttaattccgg caattccaac cagcactccc gactgtgtgg taaatcccga
      601 ttttgattta tcaacaaagt tccaggtgca tttctcatta taccttttta agagtcgcaa
      661 aaaattaaca ccccatacag ttgtatcatt ttttgaaaac cggagggctg agtagggaat
      721 tttcatctcc acgtaccaac cccggttgtc catgctaact tcgctttccc aaacggcatt
      781 ccatgagcga tcctcctcgt cggaggtaac cttgatgtcg gtttggacgc ccgaagccga
      841 aacggtaaat tgaaggtagt ttaccccatc gttataggtg ctgatgtaaa cgctaaaggc
      901 atcggacttg aggttatcgc tattgtccct ttgcccaagt tccttgtaaa tactatcggg
      961 cgatgaatcg aaaagccatg cacctacgta gagcgcatcg tcatcgtaaa gcatctttac
     1021 cctggttggt tggcttggtg atgccccatt atatggctca tactgggtaa agctttcagc
     1081 aaaaccggca agttgccatt cgggttcgtc caaatggcca tttatgctaa tatgcgattc
     1141 gatacgaaat gcctctaccg atttcctact agaaattgta tcgctgctac cccgtgcctg
     1201 ggcaaaacct gatgaaatta tgattgagga gattgtaaaa agagcgccct gtgtaaaatt
     1261 catacctgct cttgctgttt gttagaatgt ttttcagttt gagcgagaag gatacaaagg
     1321 gtttaattgt ggtaaaaaac tttccgtaac caaaaggtaa atgtccgtct ttaaaatgaa
     1381 aacgcaagtg gtaggagtga aaacttaaaa ccgtgctttc ccggaactac cttaatcggg
     1441 acgattcgcg aacaataagc tgggttttaa gaatttcggt aattggaggt tcctggcggg
     1501 tttgggtaat tcttcgaatg agctgctcca tggctttttt gcccatttca aacccaggct
     1561 ggttaacagt ggtaagggtt ggctctacca taagggatgt ttggtcatcg ccaaaaccaa
     1621 caaccgaaac atcctgagga attttaatac cattgcggat aagaatcttc atagcaccag
     1681 cagccgtaaa atcgtttacc gtaaaaatgg aatcgaactt agtcccacta tcgattagct
     1741 tctgtgtttc aatcaatgca gactggaatg tatcacaatg gataatcaac cgctcatcaa
     1801 ttaccacgcc gttatccctc atggctttga ggtagccgtt aagcctgttc tggccaatca
     1861 gcctattttg aggccccgaa aaatgaacta ttttccttcg accctggata atgagatgct
     1921 cggtagcctg gtatgctccg tcaaaatcgt caataattat tctgtcggct gaaatt
//
LOCUS       DAOOQX010000398         1968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_72397_length_1968_cov_3.658129, whole genome shotgun sequence.
ACCESSION   DAOOQX010000398 DAOOQX010000000
VERSION     DAOOQX010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72397_length_1968_cov_3.658129"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PLX53_06770"
     CDS             complement(<1..916)
                     /locus_tag="PLX53_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOY72821.1"
                     /translation="MTTKRDFYEILGVSRNATKDEIKKAYRQMALKYHPDKNPGDKEA
                     EEKFKEAAEAYEVLGDDTKRARYDQFGHSGVGNGGGGGGFTHDWTIDDIFSQFGDIFG
                     GHFGSFGGFGGFGNSRANQRATSRGSDIRVKVKLNYSEIANGVEKKLKVNKYVTCHSC
                     NGSGAASLSDFQTCSTCRGSGYVTRVTNTLLGQMQSTAPCPTCNGEGKTITKKCTECN
                     GEGVVRDNEIITIKIPAGVAEGMQLSVSGKGNAARRGGVPGDLLVVIEEEKHPQLIRD
                     GNDLIYNLTISIPDAVLGAPAEIPTVDGK"
     gene            complement(930..1550)
                     /locus_tag="PLX53_06775"
     CDS             complement(930..1550)
                     /locus_tag="PLX53_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOY72822.1"
                     /translation="MSKSSKQSKAAEDEKELETQQQVDIPEAEDKISAEGAEPNAAPA
                     SENVMEEADATDKALVEKLQKELEELKDKHLRLSAEFDNYRKRTLKEKSDMLKYSAEV
                     VLKDLLPVVDDLERALQAIESTDEITAVKEGLSLIVSKFNDFLNSKGIKEIEAMGSEL
                     NTDLHEAVAKTPVQDESQKGRVVDVIQKGYMLHDKVMRFSKVVVGE"
BASE COUNT          487 a          530 c          382 g          569 t
ORIGIN      
        1 ccttcccatc caccgttgga atttcggcag gagcgcccag aaccgcatcg ggaatgctga
       61 tggtaaggtt gtagatcagg tcgttgccat cgcggattag ctgtgggtgt ttctcctcct
      121 caataaccac gagcaagtcg ccgggcacgc cacctcgtcg ggcagcgtta cccttacctg
      181 acacgctgag ctgcataccc tcggctacac cagcgggaat tttaatggta ataatctcgt
      241 tatccctcac cacgccttcg ccattacact ccgtgcattt tttagtgatg gttttaccct
      301 caccgttgca ggtgggacat ggtgcggtgc tttgcatctg ccccaacagt gtgttggtaa
      361 ctcgtgtaac atatcctgat cccctgcagg tggagcatgt ctgaaagtcc gatagactgg
      421 cagctccgct accattgcac gaatgacagg taacgtactt gtttaccttt aactttttct
      481 ctacaccatt ggctatctcg ctgtagttga gcttaacctt aacccgtata tcgcttccac
      541 gcgaggttgc cctctggtta gccctactgt tgccaaatcc accaaatccg ccaaaactgc
      601 caaaatgacc gccaaaaata tcaccaaact gtgaaaaaat atcatcaatg gtccagtcat
      661 gggtgaaacc gccacctcca ccaccattgc ctactccgga atgcccaaac tggtcgtagc
      721 gagcacgctt ggtgtcatcg cctagcactt cgtaggcctc ggccgcctcc ttaaactttt
      781 cctcggcttc cttatcaccc ggattcttgt cggggtgata cttcagcgcc atttgccggt
      841 aggccttttt aatttcatcc tttgttgcat tccggctaac gccaagaatt tcgtaaaaat
      901 ctctttttgt cgtcatgctc tacccactcc tattcgccaa caacaacttt tgaaaaacgc
      961 atcaccttgt cgtgaagcat atatcccttc tgaatgacat ctactactct ccccttctgt
     1021 gattcatcct gaacgggagt tttggcaacc gcctcgtgca aatcggtgtt cagctccgag
     1081 cccattgctt caatttcctt aatgccctta gaattgagaa aatcgttaaa ttttgaaact
     1141 atcagtgata ggccttcctt gactgcagta atctcatcgg ttgactcaat agcttgaagg
     1201 gctctctcaa ggtcatcaac caccggaagc aaatccttca gtacaacctc ggcagagtac
     1261 ttgagcatat ccgatttctc cttcagcgtt cttttacggt agttgtcaaa ctcggccgaa
     1321 agtcgcaggt gcttgtcctt aagctcctcc aactccttct gtaacttctc aactaaggct
     1381 ttatcggttg catcagcctc ttccattacg ttttccgaag caggtgctgc attgggctca
     1441 gccccttcgg cggaaatttt atcctcagcc tcaggaatat ctacctgctg ctgggtttcc
     1501 agctcctttt catcctcagc agctttgctt tgcttgctac tcttactcat atctcaatta
     1561 tatttgtttc atttttagat ggttacacga taaaccgctt acctacaaat aatgaagtaa
     1621 tacttcatag gggaaaatcc ttgttataca caatttaaat accaagcaga aacgctcccc
     1681 caccgccatt ctgtcgccca gtccaatcaa atattattcc agcagtgcaa tggcagacaa
     1741 aatgacacaa aagtagtaaa aaatagggga tgtttatacc gatagccaac gcttaattgt
     1801 tcggctttta tcagaataaa gaaattttag cagttaaact actgcttgtt acatcagtat
     1861 tctcgagcac agatattatt atggggacaa aaaagaataa gtgataaacc cttgttctag
     1921 ggtttttcaa aatgtgccat cacgaagagc tcgttgtcaa tgcttgaa
//
LOCUS       DAOOQX010000399         1968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_72423_length_1968_cov_2.258756, whole genome shotgun sequence.
ACCESSION   DAOOQX010000399 DAOOQX010000000
VERSION     DAOOQX010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72423_length_1968_cov_2.258756"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="PLX53_06780"
     CDS             <1..1130
                     /locus_tag="PLX53_06780"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72823.1"
                     /translation="IDKEKIKILGEPLPSEGQNTINWDTDKEFELAWEIGVAPEFEIK
                     LGKKDKIPFYNIAPDDKIRKQYIDSYLMRFGHFAEADETNERGLIEATLTELNSDETP
                     REGGLFVERATISVELVADPDERAKLVGVKVGDVLTIDVQKAFPNEADRASLLSTQKE
                     NLPNIQPVFQLTVNKTSNYQPAELNQELFDRVFGEGVVTTEEEFYQKIDEEIESGLKN
                     EGENRFAVELRNILLNKIDMQLPKDFLIRWLTTINEGKFTRDQVENEYPLFEGDLKWQ
                     LIRNKIASEQNFTVTNEEMESFAMEYAQQQFMAYGMSYLPKEYIERYAKDILKNQDEI
                     RKMQEGIISRKVIAWFKENLPLDVKEITHEEFDKLEPVSGK"
     gene            1257..1940
                     /gene="clpP"
                     /locus_tag="PLX53_06785"
     CDS             1257..1940
                     /gene="clpP"
                     /locus_tag="PLX53_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOY72824.1"
                     /translation="MNEFEKYATKHRGISSLSLQRFGTITNSYISPTIIEERQLNVAT
                     MDVFSRLMMDRIIFLGVPIDDDVANIIQAQLLFLESTDPSKDIQIYINSPGGSVYAGL
                     GIYDTMQYITSDVATICTGMAASMAAVLLTAGEKGKRTALPHSRVMIHQPMGGTEGPA
                     TDIEITTREILKLRDELYEIIATHSGNPIDRIRKDSDRDYWMTAREAMEYGMIDEVLT
                     RSKNLKETK"
BASE COUNT          598 a          417 c          480 g          473 t
ORIGIN      
        1 atattgataa ggagaaaata aaaattcttg gagagccatt gcccagcgag gggcagaata
       61 ccataaactg ggacaccgat aaggaatttg agctggcatg ggaaattgga gtagcccccg
      121 agtttgagat taagcttggg aaaaaggata aaattccatt ctacaatatt gcccctgacg
      181 acaagatacg caagcaatac attgacagct acctgatgcg gtttggacat tttgccgaag
      241 ccgatgagac caacgaaagg ggattaatcg aggcaacttt aaccgagctt aatagtgatg
      301 aaactcctcg cgaaggaggt ttgtttgttg aaagagccac gatctcggtt gagctggttg
      361 ccgatcctga tgaacgtgct aagctggtag gagttaaagt gggtgatgta ctcaccattg
      421 atgttcaaaa agcttttccc aatgaggccg acagggcttc attactttct actcaaaagg
      481 agaatttgcc caacattcag ccagttttcc agctcaccgt aaacaaaact tccaactacc
      541 aaccggcgga gcttaaccag gagctgttcg acagggtttt tggcgagggg gtggttacca
      601 ccgaggagga attctaccag aaaattgacg aggaaattga atcgggactc aagaatgaag
      661 gcgaaaatcg ctttgccgtt gagcttcgga atattcttct caataaaatc gacatgcagc
      721 tcccaaagga ttttttaatc aggtggctca ccaccatcaa cgagggtaag tttacccggg
      781 atcaggtaga aaacgagtac ccgctttttg aaggggattt aaagtggcag ctaatccgca
      841 acaagattgc cagcgagcaa aacttcaccg ttaccaacga agagatggaa agttttgcca
      901 tggaatacgc ccagcagcaa tttatggcct acggtatgag ctaccttccg aaagagtaca
      961 ttgagcgcta tgccaaggat atattgaaga accaggatga aatacgtaaa atgcaggagg
     1021 gcattatcag ccgcaaggtc attgcatggt ttaaggaaaa cctaccgctt gatgtaaagg
     1081 agattacgca tgaggaattt gataagctgg agccggtttc aggaaaatag gtggctggcg
     1141 taacagcagg ttaaatagtt ttgcctcatc tcagctaatt tttgttgaaa gttctaccgc
     1201 atttaacaat taggtgctaa attgcgttac taaaccaaat ttaaaaagta ggcatcatga
     1261 atgaattcga aaaatacgca actaagcatc ggggtatcag cagcctatcg cttcagcgct
     1321 ttggaacgat aaccaacagc tacatttcac ccacaattat tgaggagcgt cagctgaacg
     1381 ttgcaaccat ggatgttttc tccaggctta tgatggatag aatcatattc cttggagtgc
     1441 ctatagatga tgatgtggcc aacattatcc aggcgcagct gctattcctg gagtcaaccg
     1501 atccttcaaa ggatattcag atatacatta actcgcccgg cggtagcgtt tacgccggat
     1561 tgggtattta tgacaccatg cagtatatca cctccgatgt ggcaacaata tgcacaggta
     1621 tggctgcatc aatggctgcg gtgttactca cagctggcga aaagggtaaa cgaacagctt
     1681 tgccccattc gcgggtaatg attcaccagc caatgggtgg gactgagggg ccagctaccg
     1741 acatcgagat taccacccgt gaaattctta agcttcgcga cgagctctat gaaattattg
     1801 ccacccattc gggaaacccc attgaccgga tacggaagga ttccgaccgc gactactgga
     1861 tgactgccag ggaagccatg gagtatggga tgattgatga ggtccttacc cgatcaaaaa
     1921 acttaaagga gactaaatga ttcctttact gcaaaaaagc gccttaaa
//
LOCUS       DAOOQX010000400         1965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_72578_length_1965_cov_2.012042, whole genome shotgun sequence.
ACCESSION   DAOOQX010000400 DAOOQX010000000
VERSION     DAOOQX010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72578_length_1965_cov_2.012042"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PLX53_06790"
     CDS             complement(<1..842)
                     /locus_tag="PLX53_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOY72825.1"
                     /translation="MLLGLILGIIAIPLGANQLVSDYIKPFGTIFLNLLKLVAMPLIF
                     ASLVTGIAGMTDVGKLSRIGIKTIAMYLITTLLAVTIGLSLVSLIKPGSYFSDDRRQE
                     FVSKYSLDISSRQQAAESISQDSPLQFLVDIVPENIVQAASSNTAMLQVIFFALLFGI
                     ALVLVRNPKVEVVNNFIQGLNEVILKIVEIIMFFAPFGVFALMASLIVDFAGDSLSSS
                     LQLFASLGMYALTIIIGLIFIIVIVYPLFLKIISGTGFGKFYKSMFPAQLVAFSTSSS
                     AATLP"
     gene            complement(1018..1551)
                     /locus_tag="PLX53_06795"
     CDS             complement(1018..1551)
                     /locus_tag="PLX53_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72826.1"
                     /translation="MHRNRLVKLLIGIVLVAFTSCGGSDDDESDNFIRFGNEKFRVYL
                     GYQYKYSETLQETGSTPFVVSLLGEGVKYSTDNAAFTGTGSLIRLYMYSESNVEVKNG
                     DYTIDIFSTKQVYSADSCSVYFNYNFDVDTGKVYPVKAGVFKVENLGRLMSYKLNMVN
                     ADSLHFVGEYKGVFNTL"
     gene            complement(1669..>1965)
                     /locus_tag="PLX53_06800"
     CDS             complement(1669..>1965)
                     /locus_tag="PLX53_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOY72827.1"
                     /translation="GYGEVDEEAIIDLPKDIFVIDGVLQEDMLVVGNTLPLRDSEGNE
                     LNGTILEVGDTHVRMDFNHPLAGEDLYFTGKIIDVREATSEELSHGHVHAHGHH"
BASE COUNT          638 a          415 c          410 g          502 t
ORIGIN      
        1 ggaagggttg ccgcgctcga actggttgag aaagccacca gctgagcagg aaacattgat
       61 ttgtagaatt ttccaaaacc cgtaccggaa atgattttta aaaataatgg gtaaactata
      121 acaataatga atatgaggcc aataataata gtgagggcat acattcccag cgaggcaaaa
      181 agctgaaggg aggaagagag gctatcccca gcaaagtcaa caataaggga agccataaga
      241 gcaaaaacgc caaaaggagc aaaaaacatg atgatttcta caatcttcag gataacctcg
      301 ttaagcccct ggataaaatt gttaacaact tcaaccttcg ggtttctaac cagcactaag
      361 gctataccga aaagcagggc aaaaaaaata acctgaagca tggccgtatt gctcgatgca
      421 gcctgaacaa tattctcagg aacaatatca accagaaact gtagtggaga atcctgagag
      481 atgctctcgg cagcttgttg ccgagagcta atgtcaaggg aatacttaga tacaaactcc
      541 tgcctgcggt catcgctgaa gtagctaccg ggttttatca ggctaaccaa gcttaggcca
      601 atggtaaccg ccagcaaggt agtgattaag tacattgcaa tggttttaat ccctattctg
      661 gagagtttgc ctacatcagt catcccggca attcctgtaa ctagcgaagc aaaaattaac
      721 ggcatggcga ctagcttcag aagattaagg aatatggtac caaagggctt aatgtagtcg
      781 gaaaccagct ggttagcgcc aaggggaatt gcaataatgc ccagtataag gcctaataac
      841 atacctatta ggattttggc atatagtggt aatcgtcggg ctcctctcat aaatatgaat
      901 gttgaaaatt tctactttaa aatcattgac ttaccctaat aaaggctact gttgttaaac
      961 atatattaaa aagaccggac aaaccggtct atagcacaag ttgagtatag gttaacgcta
     1021 aagagtattg aacacacctt tatactcgcc gacaaaatga agggaatccg catttaccat
     1081 gttaagcttg tagctcataa gccgtccaag gttctcaact ttaaaaaccc ctgcttttac
     1141 aggataaacc ttaccggtgt caacatcgaa gttgtagttg aaatagacac tgcacgagtc
     1201 ggccgaataa acctgtttgg tagaaaaaat atcaatggtg tagtctccat ttttgacctc
     1261 aacattgctc tcggagtaca tatatagcct aattagggat cctgttccgg tgaaagcggc
     1321 gttatcagtg ctgtatttta cgccctcacc aaggagagat accacgaaag gggtgcttcc
     1381 ggtttcctgc aaggtttcgg aatacttgta ctggtatcct aagtaaacac gaaacttttc
     1441 gtttccaaat cggatgaaat tatcggactc atcatcgtcg ctacctccgc atgaagtgaa
     1501 agcaacaaga actattccga taagaagttt tactaaccgg tttctatgca tacgagtagg
     1561 tatgtggttg taattaaccc tgctaaaata aaaaaaaatt attacaaaaa aggtaaataa
     1621 aaaacgggaa attaaaaatt tcccgtcagt gtttacgaat tgaagagttt aatggtgccc
     1681 atgagcgtga acatggccat ggctgagctc ctccgaagtg gcttccctga catcaataat
     1741 ttttccggta aagtaaaggt cttctccagc caaggggtgg ttgaaatcca ttctaacatg
     1801 ggtatcgccc acctccagaa ttgtgccatt tagctcgtta ccctcggaat ctctcaacgg
     1861 caatgtgttt ccgactacca acatgtcttc ctggagcact ccatcaataa cgaatatatc
     1921 cttaggaaga tcaatgattg cctcctcgtc aacttctcca taacc
//
LOCUS       DAOOQX010000401         1963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_72676_length_1963_cov_2.562893, whole genome shotgun sequence.
ACCESSION   DAOOQX010000401 DAOOQX010000000
VERSION     DAOOQX010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72676_length_1963_cov_2.562893"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLX53_06805"
     CDS             complement(<1..320)
                     /locus_tag="PLX53_06805"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOY72828.1"
                     /translation="MRRFALLLAQLTLTAGVTIAQESSKVKWYTLEEALKLNTSEPRK
                     IFIDVYTDWCGWCKKMDSNTFNNPLIASYLTKDFYAVKVKAEGKNEITYKGQVYKNQG
                     EGQRS"
     gene            687..>1963
                     /locus_tag="PLX53_06810"
     CDS             687..>1963
                     /locus_tag="PLX53_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOY72829.1"
                     /translation="MNKDIFKLEPQRLWKHFYSLTQIPRPSKKEKAAVDFMINFGRNL
                     GLETITDEVGNVIIRKPATPGMENRKTVILQGHLDMVPQKNSDKKHDFEKDPIEAYVD
                     GEWVTANGTTLGADNGIGVAAAMAVLEAKDIAHGPVEALFTIDEETGMTGAFGIKPGL
                     LKGDILLNLDSEDEGELYVGCAGGLDANIRFSFTPQPVPSDMVAYKLAVLGLKGGHSG
                     LEINLGRGNSIKLLFRFLYRACKTLGVRIGTIDGGSLRNAIPRESFAVVTVPSKNATN
                     FEKLVREYEQIYKNEFSLTEPNLSFTCKTDSLPKFLVDENSQSRAIRAFFSCPNGVVR
                     MSDAVPGLVETSNNLARVYTEGNQIVGQCLMRSSVDSAKEALADKMAAIFELADAAIL
                     FEGGYPGWKPNPNSPILTVMQQVYKNKFGTTPEV"
BASE COUNT          548 a          427 c          407 g          581 t
ORIGIN      
        1 gaccgttgcc cctctccttg gtttttatat acttgcccct tgtaggtaat ttcattcttt
       61 ccctcagcct tgaccttaac ggcataaaaa tccttggtta ggtagcttgc aatcagcggg
      121 ttgttaaagg tattgctgtc cattttctta caccagccgc accagtcagt gtaaacatcg
      181 ataaatattt tacgcggctc gctggtgttc agctttaagg cttcttcaag tgtgtaccac
      241 ttaaccttgc tactctcctg tgctatggta actccagctg ttaatgttag ctgggctaac
      301 agtaatgcaa atcttctcat acatttcaaa tgtttaccac acaaaggtaa gtaaattgat
      361 gactatggaa tttgccctaa ggctaatttt tcgtaaaatc ggcacatcaa aagcccaaaa
      421 tataggtgtt cctcccgttt ttttaacgat gcacaaaagt taaggggtaa agtaccttgt
      481 aaccagaaac ctaaaaattc ccctgattaa attttttacc tacctcaaat cataaggcta
      541 aacaaaaaaa tgcattcttc cgtttggctt tatttccgat tttttcccaa ttcatttgca
      601 cctggagcat aatggtattt tcgggatatt aaaattcttg ctatctttaa aaattcaaaa
      661 aacatgttta cctaacgtat attgctatga ataaggatat ttttaaactc gagcctcaaa
      721 ggctatggaa gcacttttac agcttgacac aaatcccccg cccatccaaa aaggaaaagg
      781 ctgctgtgga ttttatgatt aatttcggga gaaacctcgg actcgaaaca atcaccgatg
      841 aggttggaaa tgttatcata cgcaagcctg ccactccggg tatggagaat cggaagactg
      901 tgattttgca agggcacctc gatatggttc cccagaagaa cagcgataaa aagcacgatt
      961 ttgagaaaga cccaattgag gcttatgttg atggagaatg ggttaccgca aacggtacaa
     1021 ctcttggcgc cgataatggc attggtgttg ctgcagcaat ggcggtgctt gaagcaaaag
     1081 acatagctca tggccctgtt gaggctcttt tcactattga tgaggaaact ggcatgaccg
     1141 gtgcctttgg cattaaaccc ggacttctta agggtgatat tcttttgaat ttagattcag
     1201 aagatgaggg agaactctac gttggttgtg ccggtggtct tgatgcaaat atccggttta
     1261 gctttactcc tcagcctgta ccttccgata tggttgccta caagcttgcc gttcttggac
     1321 ttaagggtgg ccattcaggg cttgaaatca accttggtag gggtaattcc ataaagttac
     1381 tattccgatt cctttaccgt gcctgcaaaa cactaggcgt tcgcattggc actattgatg
     1441 gtggaagcct tagaaacgct atacctcgtg aatcattcgc tgttgttaca gttccaagta
     1501 agaatgcaac caattttgaa aagttggttc gcgagtacga acaaatctac aaaaacgaat
     1561 tttccctaac ggagcccaac ctctcattta cctgcaaaac cgattcttta cccaaattct
     1621 tagttgatga aaactcccaa tcacgggcta ttcgcgcatt tttcagctgc cctaatggtg
     1681 tggttcgcat gagcgatgct gttcccggac ttgttgaaac ttcgaacaac ctggctagag
     1741 tttataccga aggaaaccag attgttggac aatgcttaat gcgtagctct gttgactcgg
     1801 caaaggaggc attagctgat aaaatggctg caatctttga gttagccgat gcagctattt
     1861 tattcgaagg cggctacccc gggtggaaac ctaatccaaa ttccccaatc cttactgtaa
     1921 tgcagcaagt ttataagaat aaattcggaa ccactcctga ggt
//
LOCUS       DAOOQX010000402         1962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_72739_length_1962_cov_2.615627, whole genome shotgun sequence.
ACCESSION   DAOOQX010000402 DAOOQX010000000
VERSION     DAOOQX010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72739_length_1962_cov_2.615627"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..562)
                     /gene="cysC"
                     /locus_tag="PLX53_06815"
     CDS             complement(20..562)
                     /gene="cysC"
                     /locus_tag="PLX53_06815"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOY72830.1"
                     /translation="MTSCTPATIWLTGLPSSGKSTIGLGLGRMLDGIGVGYVLLDGDE
                     VRSGVCSDLGFSDDDRKENIRRVAHIAKLLNSQGLVAICCLVSPLQSMRDLARSIVGS
                     RFFYEVFVNASLETCMKRDVKGLYAKAEQGQLNNLTGFSAAYENPTDPNLVIFNNSIG
                     VEEAVNMLFAHVVDWLEIPW"
     gene            complement(632..1255)
                     /locus_tag="PLX53_06820"
     CDS             complement(632..1255)
                     /locus_tag="PLX53_06820"
                     /inference="COORDINATES: protein motif:HMM:NF027898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOY72831.1"
                     /translation="MRSLMLFVFALLSVNLVAQESQDVPQTFWDFSSKMLEVSSINAN
                     FSLTIENRQENVANTQQGEILIKGNKYLLKIMGMEVYFDGSTKWQYIGEANEVTISTS
                     GGELDSPLDDPAGIFRDYESRFKVRFISETTVKNRTLVEMDFYPKALDSPYVSVKLRF
                     DKVSLEPVFIRYHERMGQATSSTLVNLKPTSLLPMAPLHSIFQPVRA"
     gene            complement(1318..>1962)
                     /locus_tag="PLX53_06825"
     CDS             complement(1318..>1962)
                     /locus_tag="PLX53_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOY72832.1"
                     /translation="DLIMTAGREVETPLARLAQLARAIGIHLIIATQRPSTNIITGVI
                     KANFPARIAFRVPSMIDSRTILDHPGANNLVGRGDMLVSAGSDMVRVQCAFIDIPEIE
                     RITDFIGNQQGYPEAYQLPDYAPEGQDGFREIDLNKRDELFEEAARMVVQTQIGSTSL
                     IQRKLSIGYNRAGRIVDQLEAAGIVGPFEGSKARQVLIADELSLEQILRGLSNQ"
BASE COUNT          518 a          492 c          451 g          501 t
ORIGIN      
        1 acagacaaaa gggttcaacc taccatggaa tctccagcca gtcaaccaca tgggcaaaca
       61 gcatatttac cgcctcctct accccgattg aattgttgaa tatgacaaga tttggatcgg
      121 ttggattctc atacgctgcc gaaaaacctg tgaggttatt tagctgtcct tgttcagcct
      181 tggcgtaaag tcctttaaca tcacgcttca tgcaggtttc gaggctggca tttacgaata
      241 cttcgtagaa aaacctactg cccacaattg atctggccaa atccctcatg gattgaagcg
      301 gtgataccag gcagcaaatt gccactaatc cttgggaatt gagcagcttg gcaatatggg
      361 caacccgccg gatattctcc tttcggtcgt catcggaaaa gcccaggtcg gagcatacgc
      421 cgctgcgaac ctcatcacca tccagaagca catatcctac gccaatgcca tcgagcatcc
      481 ggcctaagcc caaccctatt gttgatttac ccgacgaggg tagtcctgtt agccaaattg
      541 ttgcaggtgt acagctagtc ataaggaaat aactatgtaa tgctaatgag tacagaagaa
      601 aaaattccaa cctaccgtaa atctataacc tctatgccct tacgggttga aatatcgaat
      661 gtaaaggtgc catcgggagc agggacgttg gttttaaatt tactaatgtt gacgatgtag
      721 cttgtcccat cctttcatgg tatctgataa aaaccggttc gagcgaaacc ttgtcaaatc
      781 tcagcttaac cgaaacgtag ggagagtcaa gggcttttgg gtagaagtcc atttcgacca
      841 gcgtgcggtt tttcaccgtg gtttccgata taaatctcac cttaaacctg gattcataat
      901 ctctaaaaat gccagcgggg tcatcaagag ggctgtccaa ctccccaccg gaagtggaaa
      961 tggtaacctc gttggcctcg ccaatgtact gccacttggt tgagccatca aaataaacct
     1021 ccatgcccat aatcttcagc aggtacttgt tgcccttaat cagaatttca ccctgctggg
     1081 tgttggcaac attttcctgg cggttctcga tggtaagtga aaaattggca tttatactac
     1141 tgacttccag cattttactg ctaaaatccc aaaaagtttg tggtacatcc tgggattcct
     1201 gcgcaacgag gtttaccgat aatagggcga aaacaaacag cattaaactt ctcatagcct
     1261 aattattaaa cgaaatatcg ccatttagca gccatcaagg tgtacagctg gtgtggctca
     1321 ctggttagat aaacccctca atatttgttc caaagacagc tcgtcggcaa tcagcacctg
     1381 ccgggccttg cttccctcaa acgggccaac gataccggct gcttcgagct ggtcaactat
     1441 tcgtccggct cggttatagc caatcgagag cttacgttga attagggagg tggagccaat
     1501 ttgcgtttga accaccatgc gagcagcctc ctcaaaaagc tcatcacgct tattcaggtc
     1561 tatctcccta aagccgtcct gcccttcggg tgcatagtcg gggagctggt aggcttcggg
     1621 gtagccctgc tggttaccaa taaaatcggt tattcgttca atttcaggga tatcgataaa
     1681 agcacactgc accctcacca tgtcgcttcc ggcagaaacg agcatatcgc cacggccaac
     1741 caggttattg gcccccgggt ggtcaagaat tgtgcgggaa tctatcatgg aagggactct
     1801 gaaggctata cgtgctggga agttggcctt tataacgccg gtaatgatgt tggttgacgg
     1861 ccgttgcgtg gcaataatca ggtgaatgcc aatagctctg gccagctggg ctaaacgggc
     1921 caggggtgtt tctacctccc gcccggcagt cataatcaaa tc
//
LOCUS       DAOOQX010000403         1959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_72900_length_1959_cov_3.213761, whole genome shotgun sequence.
ACCESSION   DAOOQX010000403 DAOOQX010000000
VERSION     DAOOQX010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72900_length_1959_cov_3.213761"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1722)
                     /locus_tag="PLX53_06830"
     CDS             complement(<1..1722)
                     /locus_tag="PLX53_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOY72833.1"
                     /translation="MRPSGLKRQFSRTWHGFRRLGERRIMLILSVVVGVSSGLAAVLL
                     KNVLHVANVFVQESFPKDSANFLFFALPATGILLTIVFVRFLVKDDIGHGVSKVLYSI
                     SKQNGRIKPHNMYSSMVASTSTIAFGGSVGAEAPIVYTGAAIGSNVGNFFRLNYKNII
                     LLIGCGAAGAIASIFKAPLAGIVFTLEVLMLDLTTASIVPLLISAVSAATVSYFFLGR
                     EVVLAYDVVRPFALQKIPYYVLLGVFCGFISLYFIRVVMWIEKRFERITSIYRRWILG
                     AMILGTFTFLFPPLYGEGYEVLQALLDGRSSFIFNSSPFYEFQSNVWLLLVALLILIL
                     AKGVATAVTTGAGGVGGTFAPTLFIGGVAGFFVAKLLNQLGITVVSESNFALIGMAGA
                     MAGVMHAPLTAIFLIAEITGGYALFVPLIITSTISFLTILYFEPYSIYTKNLAKRGEL
                     ITHHKDKAVLTMLSVRKVLETDFTPVEANQTLGDLVKIVGQSKRNIFPVVQDDGQLVG
                     VVTLDDIRSKMFDTSAYTVVHVQDIMTLAPAVIDVDEPMEKVMQKFEDTGAWNLPVLD
                     NGYYVGFI"
BASE COUNT          591 a          480 c          433 g          455 t
ORIGIN      
        1 aataaatccc acataatatc cattatcaag aaccgggaga ttccatgcgc cggtatcctc
       61 gaatttttgc atcaccttct ccatcggttc atcaacatca atgacggccg gggcaagagt
      121 cattatatcc tgtacatgaa caacggtata ggccgaagta tcgaacattt tagagcggat
      181 atcgtcaagg gtaacaaccc ccaccaactg gccatcatcc tgcacaacag gaaagatgtt
      241 gcgcttcgat tggccaacaa ttttaaccag gtcgccaagg gtttgattgg cttcaacagg
      301 tgtaaagtct gtttcgagca ccttgcgaac gctcagcata gtgagcacag ccttatcctt
      361 atgatgagtg ataagctcac cccttttggc aaggtttttt gtgtagatgg agtagggctc
      421 gaagtaaaga atggttagaa acgaaatggt agaggtgatg attagcggga caaaaagagc
      481 gtacccgcca gtaatctcag caatcaggaa gatggccgtt aggggtgcgt gcatcacccc
      541 ggccatggcg ccagccattc caatcagtgc aaagttactt tccgacacaa cagttatacc
      601 aagctggttt aagagtttag ccacaaaaaa acctgccacc cctcctataa agagtgttgg
      661 ggcaaaagtt cccccaacgc caccagcacc agtagtaacg gctgttgcca cacctttggc
      721 taaaattaaa atgagaagcg ccaccagcag caaccaaacg ttactttgaa attcatagaa
      781 cgggctgcta ttgaatatga acgatgaacg tccatcgagt agagcctgca gcacctcgta
      841 accctcgccg taaagcggag ggaacaaaaa ggtaaaagta cccaaaatca tagcccctaa
      901 aatccagcgc cggtatatgc tggtaatcct ttcgaagcgt ttctcaatcc acattaccac
      961 cctgatgaag tagagcgata tgaaaccgca aaacaccccc agcagcacgt agtagggtat
     1021 cttctgaagc gcgaagggac gtacaacatc ataggctaaa actacctccc gacccaaaaa
     1081 aaagtaggaa accgtagcag ctgacactgc cgaaatgagc aggggaacta tagaggcagt
     1141 tgttaaatcg agcatgagca cctctagcgt gaatactatt cctgcaaggg gcgccttaaa
     1201 aattgatgca attgctccag cggcgccgca gccaataagc aagataatat tcttgtagtt
     1261 taacctgaaa aaattcccaa cgtttgatcc tatggctgcc cctgtgtaaa ctattggggc
     1321 ttcggcgcca accgacccgc caaaggcaat agtagaggtg ctggccacca ttgagctgta
     1381 catgttatga ggctttattc gtccgttttg cttggagatt gagtaaagca cctttgaaac
     1441 gccatgccca atgtcgtcct tcaccaaaaa acgcacgaaa acaatggtta gcaggatacc
     1501 tgttgccggt agagcaaaaa ataagaagtt ggctgaatcc ttaggaaacg attcctgaac
     1561 aaaaacatta gcaacatgaa gcacattttt aagaagaact gcggccagcc cgcttgaaac
     1621 gccaaccact acgctaagaa tcagcattat tcttctttcc ccaagccttc tgaatccatg
     1681 ccacgtccta ctaaattgcc ttttcaatcc tgaaggtctc atctaaaaga tttttgaata
     1741 aactattttg ctaccaatag caaatgtagc cattattttc agcaattggt tcccgggcat
     1801 gatgttaact ggattaagcc tgcaggacct gcctggccga tttcctgggg ttataatcaa
     1861 aaatagtaaa ggatacaagg aggtgggttt cagaactcgt ccgcctgctg gcggattcca
     1921 gaacttgtcc gcctactggc ggattccaga acttgtccg
//
LOCUS       DAOOQX010000404         1954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_73187_length_1954_cov_3.202738, whole genome shotgun sequence.
ACCESSION   DAOOQX010000404 DAOOQX010000000
VERSION     DAOOQX010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73187_length_1954_cov_3.202738"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..>1954
                     /locus_tag="PLX53_06835"
     CDS             29..>1954
                     /locus_tag="PLX53_06835"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HOY72834.1"
                     /translation="MKGDGMEDNMNLKSNPQQQDLPEEEQLDVKKLLFRVLANWYWYA
                     ITLFISLFIAYLINRYSEQSFSVSSSVIVRDDDQTKSYVGAEQLIQSLRLVKNTKSVQ
                     NEIGILQSYSLARQTVEELEDFNVTYVAVGRRGIKEQKLYTGSPFKVVIDSGATNMQG
                     HPVRVNILNSREYRLEIDEGMGISRTMRFGEPFTNESFNFTVFLRDPLNYDPKVAGRK
                     YYFTINDPNTVALSYKSKVTVTLNDKKGSILTLNSTGYVAQQEADYLNKLMEVYIRRG
                     LEEKNQIAENTIRFIDRQLEDMADSLRSAELHLQDFRTSNRVLDITGEGNLLLEQIKT
                     IQNEQSSMELKARYYAYLSDYLEQRKDFNTLVAPSVMGVDDPQLNALLSEISKAYIDR
                     EELKTTVREGSPGLEQLNIRIENLRSSLAEKVKSLIESNAIAQQELNSRLSRVDAEIK
                     RLPVNERMLIGFERDFSLIDKMYTYLQEKRAEAAIARASNIPDNKVLDYAIAQNASLI
                     KPKRSTNYLIALLVGLAIPFIIMLLYDFFNDKIVDLKEISKRTRVPVIGTLGHNRYQT
                     ELPVLEKPKSTLTESFRGLRTNLQYLMREPESRVVSVSSTVVGEGKTFIAVNLAAAIA
                     VTGKRVLLLGLDLRKPKL"
BASE COUNT          538 a          523 c          486 g          407 t
ORIGIN      
        1 taaatcgcaa ctaaagggta aaaaaacatt gaagggagac ggtatggaag acaacatgaa
       61 tcttaaatcc aacccgcaac agcaggacct ccccgaggag gagcagctgg atgttaaaaa
      121 gttgctgttc cgcgtgctgg ccaactggta ctggtatgcc atcacactct ttatctccct
      181 ttttatcgca tatctcatca accggtactc cgaacagtca ttcagcgtct catcaagtgt
      241 aattgttcgc gacgacgacc agactaagtc atacgttggc gccgagcagc tcatacagag
      301 cttgcggctg gtgaagaata ccaagagcgt tcagaatgaa atcgggatac tgcagtcata
      361 ctccctggcc cggcaaaccg tggaggagtt ggaggatttt aacgttacct acgtggccgt
      421 gggccgtagg ggaataaagg aacagaagct ttacaccggt tcccccttta aggtagtgat
      481 agactccggt gcaaccaaca tgcaaggcca tcccgttagg gttaacatcc tgaacagcag
      541 ggagtaccgt ttggagattg atgagggaat gggcatttca cgtaccatga ggtttggcga
      601 gccattcacc aacgagtcgt tcaactttac agttttcctg cgcgatccct taaattatga
      661 ccccaaggtt gcagggcgaa agtactactt taccattaac gatcccaaca ccgtagccct
      721 atcgtacaag tcgaaagtta cggttaccct taacgacaaa aagggttcaa ttcttacgct
      781 aaacagcaca ggctacgtgg cacaacagga ggccgactac ctcaacaagc tcatggaggt
      841 ttacatccga cgagggctgg aggagaagaa ccagattgcc gagaatacaa ttaggttcat
      901 tgaccgccag cttgaggaca tggccgactc ccttcgcagt gcggagttac atctgcagga
      961 ttttcgcacc tccaaccggg tgctggacat aacaggcgag ggcaacctgc tgctcgaaca
     1021 aataaaaacc atacaaaacg agcagagcag catggagctc aaagcacgct actacgccta
     1081 ccttagcgac tacctcgaac agcgaaagga tttcaacacc ctggttgccc catcggttat
     1141 gggggtggac gacccacagc tcaatgccct gctttcggaa atatcaaagg cctacataga
     1201 ccgcgaggag ctcaagacta ccgttaggga gggtagcccc ggccttgaac agctcaacat
     1261 ccgcattgaa aaccttcgaa gctccctggc cgaaaaggtg aagagcctta ttgagtcaaa
     1321 tgccattgcc cagcaggagc tgaactcacg gctaagcagg gttgacgctg agattaaacg
     1381 cctccctgtc aacgaaagga tgctgatagg ttttgagcgc gacttttcgc tgatagacaa
     1441 gatgtacacc tacctgcagg agaagcgcgc cgaggctgct atagcgcgcg cctcaaacat
     1501 ccccgacaac aaggtgcttg actatgcaat tgcccagaac gcttcgctca tcaaacccaa
     1561 aaggtcaacc aactacctta tagccctgct ggtggggctg gccattccgt tcatcatcat
     1621 gctactctac gactttttca acgataaaat tgttgacctg aaggagatat ccaaacgaac
     1681 ccgggtaccc gttataggaa cgctggggca caaccgctac caaacggagc tgcccgtgct
     1741 ggaaaagccc aagtcaaccc tcacggagtc gttccgcggg ctacgaacca acctgcagta
     1801 cctgatgcgt gagcccgaat ccagggtagt ttcggtatcc tccaccgtgg tgggagaggg
     1861 taaaactttc atagccgtca acctggcagc tgccattgcc gttaccggca aaagggtgct
     1921 gctgcttggt ctcgaccttc gaaaacccaa gctg
//
LOCUS       DAOOQX010000405         1946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_73676_length_1946_cov_3.059228, whole genome shotgun sequence.
ACCESSION   DAOOQX010000405 DAOOQX010000000
VERSION     DAOOQX010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73676_length_1946_cov_3.059228"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..>1946)
                     /locus_tag="PLX53_06840"
     CDS             complement(251..>1946)
                     /locus_tag="PLX53_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOY72835.1"
                     /translation="SELAILYTGAVNVPLSVKLDGNDLLFRLVHSETSTIIVSGIQAP
                     KITRIKKEIPNLKRIIYLDSLNQYSEGEMGIEEVFKLGDTFLAEHPGRVEEVSSSVKP
                     SDFANISYTSGTTADPKGIILSHNNYTANVEQALTLMDIPQSYRTLLILPLDHCFAHV
                     AGVYAFMAMGASAATVQVGKSAIDTLKNIPINIKEIQPSLLLSVPSLAKNFKKNIEVG
                     IREKGKFTEKLFNNALKVSYRYNKEGWNKGNNFTFIYKPLIWIYDKLIFSKVRKVFGG
                     RLEFFIGGGALLDMELQRFFYAIGIPMMQGYGLSEATPIISSNSLKRHKLGSSGYLVK
                     PLDLKICDESGNELPVGQKGEIVVRGENVMVGYWKNEKATADTIRNGWLHTGDMGYMD
                     KDGFLYVLGRFKSLLISSDGEKYSPEGIEGALEEHSKFIENVILYNNQNPYTIALIVP
                     SKESIRRALNEKGIVMNTHEAGIEAIKLINNEVSRFKTGGDLQNLFPERWLPSAFSIL
                     EEPFTEDNGLINSTMKVVRGKVEEKYRDKIEHLYTVEGKNIYNNSNISSMLKIISN"
BASE COUNT          497 a          401 c          388 g          660 t
ORIGIN      
        1 cgccgcccca cctccgcccc ggccgggtta ccccgaccgg ggcttttttt tattatagca
       61 ctttgatttg tttccccggt atatacgttc tttttttatt ctcctagttg gatttaaatc
      121 ttatatgtgt ctttagatta ttaggattgg ttgtttaaat atcttctagt tttattttat
      181 acgtgtatgt tcattttaac tgactaattt tatttatcaa gctaactcag gttataatat
      241 ataaatagca ctagttgctt ataattttaa gcattgagct tatatttgag ttgttatata
      301 tattttttcc ttccacagtg tataaatgct ctattttatc cctatacttt tcttcaacct
      361 taccccgaac aaccttcatt gtgctgttaa ttaaaccatt atcttctgta aatggttctt
      421 caagaatcga aaatgccgaa ggcaaccagc gttccggaaa taagttttga aggtcgcctc
      481 cggttttaaa tcgggacact tcattgttga ttagcttgat agcttcaata ccagcttcgt
      541 gagtgttcat aacaatgcct ttttcgttta gagctctccg aatagattct tttgaaggca
      601 ctatcagcgc tatagtgtaa ggattttggt tgttatatag gataacattt tcaataaact
      661 ttgagtgttc ttcaagtgct ccttcaatgc cctcggggct gtatttttct ccatcgctcg
      721 aaatcaggag gcttttaaaa cggccaagaa cgtagaggaa accatcttta tccatgtacc
      781 ccatgtcacc tgtgtggagc caaccatttc gtatagtatc ggcagtggct ttttcatttt
      841 tccagtagcc aaccatgaca ttctcgccac ggacaacaat ttcacccttt tggccaacag
      901 gaagttcgtt gccggattca tcacatattt taagatcgag gggtttaacc aggtatccag
      961 atgaaccaag cttatgtctt tttagggagt tggagctgat aatgggcgtt gcttctgaaa
     1021 ggccgtatcc ctgcatcatg ggaataccaa ttgcatagaa aaagcgctgg agctccatgt
     1081 ctagtaacgc tccaccacca ataaaaaatt ccaaacggcc cccgaaaacc tttcttactt
     1141 tgctgaatat caacttgtcg tatatccaga ttaaaggctt gtaaataaat gtaaaattat
     1201 ttcctttatt ccacccttct ttgttgtacc ggtatgagac tttaagggca ttgttgaaca
     1261 gcttttcggt aaatttccct ttttccctaa taccgacttc aatatttttt ttgaaatttt
     1321 tggctagcga gggcacgctt agcagtaagc taggttgtat ctccttgata ttgatgggaa
     1381 tatttttgag ggtatctatt gccgatttac ctacctgcac agttgcagcg ctagctccca
     1441 ttgccataaa ggcgtaaaca cctgcaacat gtgcaaaaca gtggtcgagc ggaagtatga
     1501 ggagtgttct gtagctttga ggaatatcca ttaaggttag ggcctgctcc acatttgctg
     1561 tatagttatt atgggatagg ataatgcctt tggggtcggc agtagttccg gaggtgtagc
     1621 tgatgttggc gaagtcggaa ggttttactg atgaggaaac ctcctctact cttccaggat
     1681 gttctgctag aaaagtgtcg ccaagcttga atacctcctc tattcccatt tcaccttcgg
     1741 agtattgatt caacgaatca aggtagatta tcctttttaa gttgggtatt tccttcttaa
     1801 ttcttgttat tttaggtgcc tgtattcctg aaacaataat ggttgaggtt tcggagtgaa
     1861 ccaaacggaa aagcagatca ttgccatcca gcttaaccga cagggggacg tttactgcac
     1921 cagtgtaaag aatcgccagt tcgctg
//
LOCUS       DAOOQX010000406         1945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_73733_length_1945_cov_2.788889, whole genome shotgun sequence.
ACCESSION   DAOOQX010000406 DAOOQX010000000
VERSION     DAOOQX010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73733_length_1945_cov_2.788889"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="PLX53_06845"
     CDS             <1..1193
                     /locus_tag="PLX53_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY72836.1"
                     /translation="IYDTTGMLLATSRSEIFEKQLVGPRMNPAAWHELATNHSPKFLD
                     NENIGKAKFLSAYVPILSSNREAIAYLNLPYFTRQEELRSELYSIVVAIVNIYALLTL
                     IAISIAIIISSQITRPLQLIRERIGKVSITGNNQTINYSSDDEVGQLIGEYNRMVNEL
                     TRSANELARNQRESAWREMAMQVAHEVKNPLTPIKLSLQHLVKAKKDNLPDWDARFER
                     FAQSLLEQIESLAVIASEFSLFAKLPSAKREPVKLLDIINDTANLYRGYRNVALNVIS
                     YMEAEPLVLADKEQLIRVFNNLIKNAIQSIERGKDGKVTITLAPENDLLIRVDVTDNG
                     VGIPEEVLPKLFTPNFTTKSGGTGLGLAISREIVLGFGGEILVKTQKDLGTTFSVLIP
                     FLKH"
     gene            complement(1211..>1945)
                     /locus_tag="PLX53_06850"
     CDS             complement(1211..>1945)
                     /locus_tag="PLX53_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72837.1"
                     /translation="NLFAYKEPTIPKSNKSLIMVFKEMVLVLKDYKFILMIVVYSGFW
                     VLYFQMFDSVLWFLKEYVDMTPVDTFVNSLLAIFVSNPNWHFDAEHVTVVNAGTIIAL
                     QLVISAIVKNTRALPTMIVGIAMGTIGMAVLATSNYAWVFIGGMIVFSLGEMIAQPKF
                     ISYIGLIAPDDKKALYMGYSFLYGVIGSGIGGVLGAYSYVKLVNEMGRPSMFWLMFSF
                     IGVATILGLLAYNRFILKKGTANPHS"
BASE COUNT          563 a          456 c          437 g          489 t
ORIGIN      
        1 atatttacga cacaaccgga atgctgctgg ctacatcgcg ttccgaaata tttgaaaagc
       61 agctggttgg accaagaatg aaccctgcgg catggcatga gctggcaacc aaccacagcc
      121 cgaaattcct tgataacgaa aatataggaa aggctaagtt tctttcggct tacgttccga
      181 tactaagttc gaatcgtgag gcaatcgcct accttaacct accctacttt acacgccagg
      241 aggaactacg ctccgagctc tactccattg tggtggcgat tgtcaacatt tacgctctcc
      301 ttactcttat agcaatttcc attgccatta tcatttcgag ccagataaca cgtcccctac
      361 agcttattcg agagcgtata ggcaaagtaa gcattacagg gaacaaccaa accattaact
      421 acagtagcga tgatgaggtt ggccagctaa ttggagagta caaccgcatg gttaacgagc
      481 ttacccgcag cgccaatgag ctggctagga accagcgtga gagtgcatgg cgcgagatgg
      541 ccatgcaggt tgctcacgag gtgaaaaacc ccttaactcc aataaagtta agcctacaac
      601 atcttgtgaa ggccaaaaag gataacttgc ccgactggga tgcccgattc gagcgttttg
      661 cccaatcact tttggagcag atagaatctc tggcggtgat agcaagcgag ttttccctat
      721 ttgccaagct tccttctgca aaaagggagc cggtaaagct gctggatata ataaacgaca
      781 ctgccaacct ttacagggga tataggaatg ttgcgctcaa cgttattagc tacatggagg
      841 ctgaaccgct ggtgctggcc gacaaggagc agcttatcag ggtttttaac aacctcatta
      901 aaaacgccat tcagtccatt gagcggggta aggatggaaa agttaccata acccttgccc
      961 ctgaaaatga tttgttgata agggttgatg ttaccgacaa tggggtggga attcccgaag
     1021 aggtgctacc caagctgttc acacctaatt ttaccacaaa gtccggggga actggtcttg
     1081 gcctggccat ttcgcgggag attgtattgg gctttggggg agaaatacta gtcaaaaccc
     1141 aaaaggattt aggcacaacc ttttcagttt tgataccgtt tttaaagcat tagcttctgt
     1201 ttttgaatta tcagctatgc ggattggcag ttcccttttt tagaataaac ctattgtagg
     1261 ccagcaagcc caaaattgtt gccacgccta taaagctaaa catcagccaa aacattgagg
     1321 gccgacccat ttcgttcacc agcttaacat acgaataagc gcctagcacg cccccaattc
     1381 cactgccgat aactccatag aggaacgagt agcccatgta gagtgctttc ttgtcatcgg
     1441 gtgcaattag cccgatgtag gagataaact tgggttgggc aatcatctcc ccgagcgaaa
     1501 agactatcat tcctccaatg aaaacccagg cataatttga cgttgcaaga acggccatgc
     1561 ctattgttcc catggcaatc cccacaatca tggtaggtaa tgcccgggtg ttttttacaa
     1621 tagccgaaat aaccagctgc agggcaatta tggttccggc atttacaacg gttacatgtt
     1681 ctgcatcgaa atgccaattg gggttgctta caaaaatggc aagcaatgag tttacgaaag
     1741 tatcaactgg cgtcatgtca acgtactctt ttagaaacca aagaaccgag tcaaacatct
     1801 ggaagtagag aacccaaaag cccgagtaaa ccactatcat aaggataaac ttgtagtcct
     1861 ttaaaaccag caccatttcc ttgaatacca ttatcaggct tttgttactc tttggtatgg
     1921 ttggctcctt ataagcgaag aggtt
//
LOCUS       DAOOQX010000407         1941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_73956_length_1941_cov_3.106575, whole genome shotgun sequence.
ACCESSION   DAOOQX010000407 DAOOQX010000000
VERSION     DAOOQX010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73956_length_1941_cov_3.106575"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PLX53_06855"
     CDS             complement(<1..843)
                     /locus_tag="PLX53_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOY72838.1"
                     /translation="MRSLEKLYSELEARNQAAEMAGGQKRIDKQHEGGKKTARERIVE
                     LLDPGTFTEIDKFVTHRTVEFGMADKHIPGDGIVAGYGKIDGRRVYVYAYDFTVFGGT
                     LSRANADKIIKLQQLALKMGVPVIGLNDSGGARIQEGVQSLAGYAEIFYNNVRSSGVI
                     PQITCIMGPCAGGAVYSPALTDFIVMVKDTSYMFVTGPDVIKAVTHEDVTKDELGGAV
                     THNSKSGVAHFIADNDEQALMLVRELMSFLPINNMEEPPIVPTTDDSTREDELLQTLI
                     PADPN"
     gene            complement(924..1433)
                     /locus_tag="PLX53_06860"
     CDS             complement(924..1433)
                     /locus_tag="PLX53_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOY72839.1"
                     /translation="MGLEVKIGDRVADVKLVKREGQQVTIEVDGKLYEVDIAQVENGV
                     YSILHNGKSYNIEIIPGDGPKSYFVNTFYNSHEVEIIDAHSRYMMSRNLSSENAADRV
                     ISTPMPGRVVKIPVTEGEQVSKGATVIVISAMKMESEYKSPVDGTIRKIHVAEGDNIA
                     ANQPLIEIE"
     gene            complement(1444..>1941)
                     /locus_tag="PLX53_06865"
     CDS             complement(1444..>1941)
                     /locus_tag="PLX53_06865"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014804.2"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOY72840.1"
                     /translation="SAEDPFNNFMPSPGLVRHISEPNGLGIRTDGYVYEGYEIPIHYD
                     PMISKLISWGRTREEAVDRMKRALFEYKITGVKTSLRFLSRIMENKDFRNGVYNTHFV
                     PDHFDELIGGEATEKPIYEDMAVIAAYFDFTSKMERVAMNDFSIQHKPDSWKVYGINR
                     NLMRI"
BASE COUNT          467 a          500 c          437 g          537 t
ORIGIN      
        1 attcgggtcg gcgggaatca gggtttgcag tagctcatcc tcgcgggtag aatcgtcggt
       61 tgttggcaca attggaggct cctccatgtt atttatgggc agaaaactca tcagctcccg
      121 tacaagcatt agggcctgtt cgtcgttgtc ggcaataaag tgggccaccc cgcttttaga
      181 gttgtgggta accgcaccgc ccagctcgtc cttggtaaca tcctcgtggg ttacagcttt
      241 aatgacgtcg gggccggtta caaacatgta gctggtgtcc tttaccatca cgatgaaatc
      301 ggttagagcg ggtgagtaaa cagctccgcc tgcacacggc cccatgatgc aggttatttg
      361 aggaattacc ccggagctcc tcacgttatt gtagaatatt tcagcatatc ccgcaagact
      421 ttgcacgcct tcctgtatgc gggcgcctcc ggaatcgttg agcccaatga ccggaacacc
      481 catttttaag gccagctgct ggagcttgat aatcttgtcg gcattggcac ggctcagcgt
      541 tcctccaaat accgtgaagt cgtaggcata aacgtaaacc cgtcgcccgt cgattttacc
      601 gtatccggct acgattccat caccgggtat atgcttatcg gccatgccga attctactgt
      661 ccgatgggtt acaaacttgt ctatttcggt gaacgttccc ggatcaagca gctcaacaat
      721 acgctcgcgg gcggttttct ttccaccctc gtgctgctta tcaatacgtt tttgaccacc
      781 ggccatttca gccgcctggt tacgagcctc cagctcgctg taaagttttt ctaaagacct
      841 cataaaaaga tggttaaatt tggcaggtag tagcttgacc taccgcaaaa tgaatcataa
      901 agaataagta gcccttaata gagctactca atctcgataa ggggttgatt tgcagcgata
      961 ttatcaccct cggcaacatg aatctttcgg atagttccat ccacggggct cttatactcg
     1021 ctctccattt tcatggccga aattacgatt accgttgctc cttttgacac ctgctcgcct
     1081 tcggttacag ggattttaac caccctaccg ggcattggtg ttgaaataac cctatctgct
     1141 gcattttcag acgataggtt tcggctcatc atgtaacggc tgtgggcatc tataatctca
     1201 acctcatgcg agttgtagaa ggtattcaca aagtaacttt taggaccatc gccagggata
     1261 atttcaatgt tgtacgattt gccgttgtgc aaaatggagt aaactccgtt ttccacctgg
     1321 gcaatatcca cctcgtagag tttcccgtca acctcaatgg taacctgctg gccttcccgt
     1381 ttaacaagct ttacgtcggc aacccgatcg ccaattttta cctcaagtcc catcctctga
     1441 aagttatatg cgcatcaagt ttctattaat accgtaaacc ttccagctgt cgggcttatg
     1501 ctgaatggaa aagtcgttca tggcaacgcg ctccatcttg gaggtaaaat cgaagtaggc
     1561 agcaataacg gccatatcct catatatggg tttttctgta gcttcaccgc caatcaactc
     1621 gtcaaaatga tcgggtacaa agtgggtgtt gtacacccca ttgcggaaat ccttattttc
     1681 cattattctg gaaaggaagc gaagcgaggt ctttacaccg gtaatcttat actcaaaaag
     1741 tgcccgcttc atgcggtcaa ccgcctcctc gcgagttcta ccccacgaaa tcagcttgct
     1801 aatcatagga tcatagtgaa tggggatctc gtatccctcg taaacataac catcggttct
     1861 aattcccagc ccgttgggtt cacttatatg ccgcactagt ccgggtgatg gcatgaagtt
     1921 attgaaggga tcttcagcag a
//
LOCUS       DAOOQX010000408         1940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_74000_length_1940_cov_3.881698, whole genome shotgun sequence.
ACCESSION   DAOOQX010000408 DAOOQX010000000
VERSION     DAOOQX010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74000_length_1940_cov_3.881698"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PLX53_06870"
     CDS             complement(<1..304)
                     /locus_tag="PLX53_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOY72841.1"
                     /translation="MGQIVNTDKRKDLGTLGEDSAAEYLVSKGYKILHRNWHFGHKEL
                     DIVARDGNMLVVVEVKTRTSDYWEEPKEAVRRKKQKRLVEAADAYVCHYNLDTEVRF"
     gene            409..1083
                     /locus_tag="PLX53_06875"
     CDS             409..1083
                     /locus_tag="PLX53_06875"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HOY72842.1"
                     /translation="MIRLKEQKWGHFRRFILVIPMLLLFPFNLLSNIDNFEGKIKFLS
                     EGVYDTLSMVLSVKNDLVRMDQYSGKNGLEISYLFDLRSGKVFALSGREKVYKEIYLH
                     DQSKRGETDVTIIKSQNSMLINGTTCYQWRVKNRQSMTEVTYWVAQSGFDFMEPLQRL
                     IGQLQTPFNLFTLCPDIKYFFPLMVVDRTIFRKFRCSLRVVDIKRVVLPDNLFAIPRG
                     YRELLG"
     gene            complement(1049..>1940)
                     /locus_tag="PLX53_06880"
     CDS             complement(1049..>1940)
                     /locus_tag="PLX53_06880"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOY72843.1"
                     /translation="ARFVSHEDVNPAIRFLTARGFIVKTAPSLFAKHFQFAGSDSERA
                     SDFMEMVNDSNVKAILCARGGYGTVRILDRINLRKLQLKPKWIVGYSDITVLHSIITS
                     WFGIETLHATMPVNFPKNINDSESASSMVDVLMGRNPAYSIPHHPLNRKGTATGFLTG
                     GNLSILASLIGTDADTSTQERILFIEDVDEYLYHVDRMMLQLKRAGKLKSIAGLVVGH
                     FSEMKDNNIPFGLDAYQIISEAVKEYRIPVCFGFPAGHQEPNLPLIMGRKVQLVVNSQ
                     GASLTFHESSSQAILDTHGE"
BASE COUNT          526 a          463 c          419 g          532 t
ORIGIN      
        1 cgaaacgcac ctcggtatca aggttgtagt ggcaaacgta agcatcggca gcctccacca
       61 gccgcttttg ctttttcctt cgaacggctt cttttggttc ctcccagtag tcgcttgtcc
      121 gagttttaac ctccacaacc accagcatat tcccatctct ggccacaata tcaagctcct
      181 tgtgcccaaa atgccaattt cggtgtaaaa ttttataccc ttttgatacg aggtactccg
      241 ctgccgaatc ctcgcccagt gtacctaaat cctttcgttt gtcggtgtta acaatttgcc
      301 ccatttcggg tgaaacatta tttagcgtta tgatgtataa acattacact ttaggttgaa
      361 aaccataaag gtcaaaactc acttacttca acagctaaca taaacctaat gatacggttg
      421 aaagaacaaa aatggggaca tttccgtcga tttattctgg ttatacccat gttgttgctt
      481 ttcccattca acctgctatc aaacatagac aattttgagg gaaaaatcaa gtttttaagc
      541 gagggggttt acgacacgct gagcatggtt ctcagcgtaa agaatgattt ggtcagaatg
      601 gaccagtact ccggcaaaaa tggtctggaa atatcctacc tcttcgacct gagaagcggt
      661 aaggtttttg ctctttcagg cagggaaaaa gtgtataagg aaatctacct gcacgatcag
      721 tctaaaagag gggagacaga cgtaaccatt atcaagagcc aaaactcaat gttgataaat
      781 ggaacaacct gctaccaatg gagggttaag aataggcaga gcatgaccga agtaacctac
      841 tgggttgccc aaagtggctt tgacttcatg gagccccttc aacggcttat tggccagctg
      901 caaacgccat tcaacctgtt tacgctttgc cccgacatca agtatttttt cccgctgatg
      961 gtggttgacc gaaccatatt ccgaaaattc aggtgcagcc taagggttgt agatataaaa
     1021 cgggtcgtac tccccgataa tttgttcgct attccccgtg ggtatcgaga attgcttggc
     1081 tagaactttc gtgaaaggta agcgaggcac cctgagagtt taccacaagc tgcactttac
     1141 gtcccatgat caatggaagg ttgggttcct gatgacccgc cggaaaaccg aagcataccg
     1201 gtatcctgta ctccttaacc gcctcactaa ttatctggta tgcgtccaac ccgaatggga
     1261 tattgttgtc cttcatttct gagaaatgac ccaccaccaa tccagctata gacttgagct
     1321 ttccagcccg tttaagctgg agcatcatgc ggtcaacatg gtagaggtac tcatcaacat
     1381 cctcgataaa aaggatgcgc tcctgtgttg aagtgtcggc atcggttcca ataaggctgg
     1441 ccagtattga aaggttacct cctgttagaa aacctgtagc cgttcctttt cgatttagag
     1501 ggtggtgtgg aatgctatat gccgggtttc tccccattaa aacgtccacc atgctgctag
     1561 ccgattcgct atcatttatg ttttttggaa agttaacagg catggtagca tgaagggtct
     1621 caatgccaaa ccagctggtt ataatgctat gaagaacagt aatatcgcta tatcccacta
     1681 tccatttggg tttaagctgg agcttacgca ggtttatcct gtccaaaatt cttaccgttc
     1741 cgtatcctcc acgagcgcaa agaattgcct ttacgttcga atcattaacc atctccatga
     1801 aatcggaagc gcgttcgcta tccgaccctg caaactgaaa atgtttggca aagagggagg
     1861 gtgccgtttt cactataaaa ccccgtgcag tcagaaaccg gatggccgga ttaacatctt
     1921 cgtggctgac aaaccttgca
//
LOCUS       DAOOQX010000409         1940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_74052_length_1940_cov_2.162334, whole genome shotgun sequence.
ACCESSION   DAOOQX010000409 DAOOQX010000000
VERSION     DAOOQX010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74052_length_1940_cov_2.162334"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="PLX53_06885"
     CDS             <1..1165
                     /locus_tag="PLX53_06885"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOY72844.1"
                     /translation="NLSGTGNNKRNFTLLGDPALMLRYPELGAEITSINGKPVDEEAD
                     TLKALSVANLSGRIFTPIGQVASDFSGNATITVFDKKSEIKTLANDGGMPMTFFSWDN
                     VLYKGRAIVNNGLFSIDFVVPKDIRLSFGTGRISIFADDLARVAMGYNESVIVGGVFN
                     SPYTDNEGPEIDVYLNDTLFNGGGISDSSPKLLIRLRDESGINTSGIGIGHDLTATLT
                     SPGGDEVNVILNMYYQSEPNSYQEGYVNYQLADLSEGRYWLRVKAWDTYNNSSEKIIS
                     FVVVGAGNLQLEKFYSYPNPFSEGTSFYFEHNMPEDEFNLTIQIFDVAGRLVSTITQS
                     QAYSGGYRVGPIYWNGTDSRGSRLGRGIYICRLSVKTSSGYAASLQHKLVILR"
     gene            1241..>1940
                     /gene="porV"
                     /locus_tag="PLX53_06890"
     CDS             1241..>1940
                     /gene="porV"
                     /locus_tag="PLX53_06890"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOY72845.1"
                     /translation="MRRILFTLLSFVGFIFFSQVSSAQFDSTVSLTGGLNALKIAVPF
                     LTIAPDSRAGGMGDAGVATSPDINSQHWNPAKFPFIESKKWGVAFSYTPWLRNLVNDI
                     NLTYLTGYYQVDKMQAISSSLLYFAMGEIQFTTDNPNDPPVKFFNPNEFAIDIAYSRK
                     FSDYISGAIAFRYIRSDLSGGYSQPGQASAKAGTSFAADIAMYYQHPVKLSDKFGQMA
                     FGFNISNIGTKLSYS"
BASE COUNT          525 a          440 c          429 g          546 t
ORIGIN      
        1 aaacctttcc ggaacgggta acaacaagcg caactttacc ctgctgggcg atccggcttt
       61 aatgcttcga taccccgaac ttggagctga aattaccagc attaatggaa aacctgttga
      121 tgaggaggcc gataccctca aagctttatc ggttgcaaac ctctcgggaa ggatatttac
      181 cccgattggc caggttgcat cagactttag cggaaatgcc accataactg tttttgataa
      241 gaaaagcgaa attaaaacgc tggcaaatga tggcgggatg ccaatgacct tttttagttg
      301 ggacaatgtg ctctataaag gcagagctat tgtcaataac ggattattca gcattgattt
      361 tgttgtacca aaggatattc gcctatcgtt tggcacgggt aggataagta tttttgctga
      421 cgacttggca agggtggcaa tgggctacaa cgaatcggta attgttggcg gggttttcaa
      481 ctcaccttat accgataacg aaggaccgga gattgacgtt taccttaacg atacactttt
      541 caacggggga ggaatatccg atagcagccc taagcttctt atccggttaa gggacgagag
      601 tggtattaat acttccggaa ttgggattgg acacgattta acggcaaccc ttacttcgcc
      661 tggtggcgat gaggttaacg taattctaaa catgtactac cagtccgaac ctaacagcta
      721 ccaggagggg tatgttaact accagctagc tgacctttcc gaaggaagat actggcttag
      781 ggtcaaggca tgggatacct ataataatag ctctgaaaaa attatttcgt tcgttgtggt
      841 tggagcagga aaccttcagc tggaaaaatt ctactcttac cctaacccat tttctgaggg
      901 aacatctttc tactttgaac ataacatgcc ggaagatgag ttcaacctta caattcaaat
      961 ttttgacgtg gcaggacggc tggttagcac aattacacag tcacaggctt actctggggg
     1021 ataccgggtt ggacctattt actggaatgg aactgacagc aggggaagtc ggcttggaag
     1081 gggcatatac atctgccgct tatccgttaa aacttctagc ggatatgcag ccagcttaca
     1141 gcataaactt gtcattttga ggtaaaatat aattccgagt tttacgttcg ctagaatata
     1201 tttcacaatc agctaaattt gcacctaaaa tttatatcag atgcgtagaa tactgtttac
     1261 acttctttct tttgttggct tcattttttt ttcacaagtc tcaagtgcac agttcgatag
     1321 cactgtttcg cttaccggcg gactgaacgc tctcaagatt gccgttcctt ttctaactat
     1381 tgcacccgat tcaagagctg gcggaatggg cgatgctggt gttgccactt cacccgatat
     1441 caactcccag cattggaatc cggctaagtt cccatttatc gaaagtaaaa aatggggtgt
     1501 tgccttttcc tatacccctt ggcttagaaa tttggtgaat gatatcaacc ttacctacct
     1561 caccggttac taccaggtgg acaagatgca ggccatcagc agctcgctgc tatacttcgc
     1621 catgggcgaa atccagttta ctaccgacaa ccccaatgac cccccggtta aattttttaa
     1681 ccctaacgag tttgccattg acattgctta ctcccgcaaa ttttccgatt acatttcggg
     1741 tgctattgcc ttcaggtaca tccgttccga cttgagcggt ggctactcgc aacccgggca
     1801 agcctcggct aaagctggaa cttcctttgc agccgatatt gctatgtact atcaacatcc
     1861 tgtaaagcta agcgataaat ttgggcaaat ggcttttggt ttcaacatct ccaacatcgg
     1921 cacaaaactt tcatacagcg
//
LOCUS       DAOOQX010000410         1935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_74343_length_1935_cov_2.180319, whole genome shotgun sequence.
ACCESSION   DAOOQX010000410 DAOOQX010000000
VERSION     DAOOQX010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74343_length_1935_cov_2.180319"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLX53_06895"
     CDS             <1..531
                     /locus_tag="PLX53_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY72846.1"
                     /translation="YLLGSNGNKEKAQRYIDLAEQHANNILKIKPGHVGAMAMNSALL
                     AFKVYLSPYKAPFLGPKSIKIIDEALKLDANNPYPLLEKANFLQYAPGVVGGNPTEAL
                     GYYQKATEILRKGNNGQHPKTWWYMNAWAQMALSAEKAGKIDLARKTFKEILAISPDF
                     KWVKTKLYPDFVKKHG"
     gene            complement(779..1003)
                     /locus_tag="PLX53_06900"
     CDS             complement(779..1003)
                     /locus_tag="PLX53_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOY72847.1"
                     /translation="MKTEQFSVGKMKCMGCVATITSGLSEIEGVSKVNAELESATVTV
                     TYENDGLREVIINKLEALGYPVNLDIKQGS"
     gene            complement(1236..>1935)
                     /locus_tag="PLX53_06905"
     CDS             complement(1236..>1935)
                     /locus_tag="PLX53_06905"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOY72848.1"
                     /translation="RIPTLRSILIHMWDKASQYLNKIGGVILVGVIIIWALGYFPRNV
                     QTSKDYDSLIGLKRAALTSAQATNAATAESLEQEIQEIEMEREALRQEQSYLGRIGKF
                     IEPALKPLGFDWKMGVSLISGIAAKEIVVSTLGVLHLAKEPNGSQSLPDKLKADIYTT
                     GEKVGQKVFTPLSSFSFLIFILIYFPCVAVISAIKQESGSWKWALFTIVFTTALAWVS
                     SFLFFQVGTLITGS"
BASE COUNT          565 a          418 c          388 g          564 t
ORIGIN      
        1 tacctgcttg gttcaaatgg aaacaaggaa aaggctcaac gctacatcga cttggccgag
       61 cagcatgcaa acaacattct aaagattaaa cctggacatg ttggtgccat ggcaatgaat
      121 tctgccctgt tggcttttaa agtttattta agcccatata aggctccttt tttagggcca
      181 aaaagcataa aaataattga tgaggcgctt aagctcgatg ccaataatcc gtaccccctt
      241 ttagaaaagg ccaatttttt acagtatgct ccgggtgttg tgggaggaaa ccctaccgaa
      301 gctttaggct actaccaaaa agctaccgaa attcttcgaa aaggtaataa cggacagcat
      361 cctaaaacgt ggtggtacat gaatgcctgg gcgcaaatgg cactttctgc tgagaaagct
      421 ggaaaaattg atttggcccg taaaactttt aaggaaattt tggctatttc acccgatttt
      481 aaatgggtaa aaaccaagct gtaccccgac ttcgtgaaaa aacacgggta aaggcagctt
      541 gactggtaaa atgtttctac ttgaaatttg aaacgcccat gtttttgcga atactaacac
      601 gatagaagta aaattatcaa catgggtgtt ctaaccacta agttcccatg cataaaagca
      661 tggactaaag tatttgcaat cggaaaccta ggtgtttttg taattaaggg ataccaaaag
      721 aggaagtgat accttcactt cctctacctg aatacatgaa cgtgcagatg gttttcggtt
      781 aggagccttg cttaatatca aggttaaccg gatacccaag ggcttcgagc ttattgatta
      841 ttacctctcg taaaccatca ttctcatagg ttaccgttac agttgccgat tcaagttcgg
      901 catttacttt tgatactcct tcaatttcag aaagaccgct ggtgattgta gctacacatc
      961 ccatacactt catctttccc actgaaaatt gttctgtttt catggttgct ataaatttag
     1021 ttttaagttt cacataattt gtaatgccat tcgtttgaca ctcaattagt agagcttact
     1081 tactcttcaa ctccctaatt ccacaagatg aggagctgca tccggcacaa tggcccaaat
     1141 tcttttgctt tggcctgaat acccggtaaa tgctgtatcc tacatagagg attgctgcta
     1201 ttatcaacat atatactaca atttcctgaa ccatactagc ttcctgtaat taaagtccca
     1261 acctgaaaga atagaaacga tgaaacccac gccagcgcag tggtgaaaac aatggtaaaa
     1321 agagcccatt tccacgatcc tgattcttgt ttaatagccg atattactgc tacacagggg
     1381 aagtaaatta gaataaatat taaaaaggag aatgaactaa ggggcgtgaa taccttttgg
     1441 ccaacctttt ccccagtagt gtaaatgtcg gccttaagct tgtcgggtag cgattgtgaa
     1501 ccatttggct ctttggccag atgaagtacc cccagggtgc ttaccacaat ttccttggca
     1561 gcaatccccg aaatcaggct aactcccatt ttccaatcga atccaagcgg tttgagggca
     1621 ggttcgatga actttccaat tcttccaagg tacgactgtt cttgtcgcag ggcttccctt
     1681 tccatctcga tttcttggat ttcttgctcc aggctctctg ctgtggcagc gttggtggct
     1741 tgtgctgaag ttagagcagc acgtttaagc ccaatcaaac tgtcataatc ttttgatgtc
     1801 tgtacatttc gtggaaagta tcctaaagcc catataatga taacccctac cagaattact
     1861 cctccgattt tattaaggta ctgggaggct ttatcccaca tgtgaataag tatcgaacgt
     1921 agggttggaa ttcgg
//
LOCUS       DAOOQX010000411         1931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_74558_length_1931_cov_3.030384, whole genome shotgun sequence.
ACCESSION   DAOOQX010000411 DAOOQX010000000
VERSION     DAOOQX010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74558_length_1931_cov_3.030384"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLX53_06910"
     CDS             <1..336
                     /locus_tag="PLX53_06910"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOY72849.1"
                     /translation="LQIFGQCSHPNWHGHNYELFVTVKGEVNADLGYAVNAKDISRIV
                     HDMVVLKLDHKNINMDVDFMRGKMASTENLAVSIWNELQKPLAVVGALLHCIRIVETE
                     NNSVEYYGE"
     gene            350..988
                     /gene="folE"
                     /locus_tag="PLX53_06915"
     CDS             350..988
                     /gene="folE"
                     /locus_tag="PLX53_06915"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOY72850.1"
                     /translation="MEEINQGASINDEEGYSRIERWNTETTEKLAYHYSEIIKLIGED
                     ASREGLVQTPVRVAKSIQFLTHGYNVDPHEMIRSARFKEDYRQMVLVKDIELYSLCEH
                     HMIPFYGKAHVAYIPNGYITGLSKIARVVDAYARRLQVQERLTVQIRDCIQETLNPLG
                     VAVVIEAAHMCMQMRGIQKQNSVTTTSAFTGAFLTHDNTREEFIHLIGVRLH"
     gene            complement(1057..1782)
                     /locus_tag="PLX53_06920"
     CDS             complement(1057..1782)
                     /locus_tag="PLX53_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOY72851.1"
                     /translation="MKRLLFTLVIVMAYGISNAQLFKVFGHEVGYIYVGPRLGMNFSK
                     FSDFNGTYEKSRFGYEFGAVGEFGFTTRFSIQTELMFLSKGSRDENFGKIRMNYIGIP
                     ILAKYGFRALGLDKVYVMGGTFANVRVGGAWVYDDGTTYELGGGMKKFEWGMGLGAGA
                     QYPFKTGVAALDLRYSLGFSDIHSDDNITTHSRSFEVILTYKFDLVDLMFKLKKNKTP
                     EPEPAGNEVDSNNAKGLKIEKRN"
BASE COUNT          536 a          446 c          421 g          528 t
ORIGIN      
        1 cttcaaattt ttggtcagtg ctcgcatccc aactggcatg ggcataacta cgagctattt
       61 gttacagtta agggcgaggt taatgcggat ttggggtatg cggtgaatgc caaggatatt
      121 agccggattg tgcatgacat ggtagtgctg aagcttgacc ataagaacat caacatggat
      181 gttgatttta tgaggggcaa aatggcctca accgaaaacc tggctgtttc aatctggaat
      241 gagctccaaa aacctttagc ggttgttggt gctttacttc actgcatcag gatagtagaa
      301 acagaaaaca actctgtaga gtactacggg gaataaattt attgtggaaa tggaagaaat
      361 taaccagggc gcatctataa acgatgagga gggttactcc cggattgagc gttggaacac
      421 cgagacaacc gaaaaacttg cctaccacta cagtgagata atcaagctta ttggcgagga
      481 tgcctcgcgg gagggcttgg ttcaaactcc tgtaagggtg gctaagtcca ttcagttcct
      541 tacgcacggc tataatgttg acccacatga gatgatacga agcgccaggt ttaaggagga
      601 ttaccggcag atggtgctgg tgaaggatat cgagctctac tcgctgtgcg agcaccacat
      661 gatacccttt tacgggaaag ctcatgttgc ctacatccca aatgggtata tcaccggttt
      721 aagtaaaatt gccagggttg tagatgccta tgcccgaagg cttcaggttc aggaacggct
      781 taccgttcaa atccgcgact gcattcagga aacgctcaac ccattgggag tggccgtggt
      841 gattgaagct gctcacatgt gcatgcagat gcggggcatt cagaagcaga actccgtaac
      901 caccacttct gcattcaccg gtgctttcct aacccacgat aacacccgcg aagagtttat
      961 ccacttgatt ggtgtcaggc tgcactagct tatcactttt aacaataaaa gcctgatggc
     1021 aggcttctac aaaataaata aggtaatagc taagggttag tttctttttt caatcttcag
     1081 ccccttggca ttgttgctat ccacttcgtt acctgctggt tcaggttccg gagttttatt
     1141 cttcttcaac ttgaacatca ggtcaaccaa atcgaacttg taggtgagaa ttacctcgaa
     1201 tgatctggag tgagtcgtaa tattatcatc cgaatgaata tctgagaacc ccaatgagta
     1261 ccttaaatcc aaagctgcta caccagtttt aaacgggtac tgcgctcctg cacccagccc
     1321 cattccccac tcgaattttt tcataccacc tcccagttca taggtagtgc catcgtcata
     1381 tacccaagcg ccgccaaccc ttacgttggc aaacgttccg cccatcacat aaaccttgtc
     1441 cagccccaac gccctgaatc cgtatttggc cagaatgggt atgccgatgt agttcatcct
     1501 gatttttcca aaattctcat ccctgcttcc cttcgacagg aacatcagct cggtttgaat
     1561 ggaaaaacga gtggtgaaac caaactcccc cactgctcca aactcatatc caaaccggct
     1621 tttttcataa gtgccgttaa agtcggaaaa cttggaaaag ttcatgccaa gcctgggtcc
     1681 aacgtaaata taccctacct cgtgtccaaa aaccttgaac agctgtgcat tcgagatgcc
     1741 ataagccatt actatgacca gggtgaatag tagcctcttc attattaggt taatttttta
     1801 ggttatctag taacacaaat taacaaatat tttttgaaac cgatagcggg tttgaaatga
     1861 aaaatttcat ttttggttgg ttagcaaata tttgctgata ttttattcac ttatggttta
     1921 acccgttcat a
//
LOCUS       DAOOQX010000412         1930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_74640_length_1930_cov_2.534400, whole genome shotgun sequence.
ACCESSION   DAOOQX010000412 DAOOQX010000000
VERSION     DAOOQX010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74640_length_1930_cov_2.534400"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLX53_06925"
     CDS             complement(<1..336)
                     /locus_tag="PLX53_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOY72852.1"
                     /translation="MPRILIIGKNSFIGKNYREYSKNSSIDEVDAKQYKPEELNFEGY
                     DVVLHLAAIVHQSGNIGLEEYTRVNTHLPVALAEQAKMAGVGQFIFMSTSKVYGKYNP
                     AMGSWTEDSP"
     gene            complement(385..1452)
                     /locus_tag="PLX53_06930"
     CDS             complement(385..1452)
                     /locus_tag="PLX53_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOY72853.1"
                     /translation="MDSNSKIYIAGHRGLVGSALERKLRQKGYYNIVVRTHAELDLLC
                     QQDVFNFFASEKPEYVFMAAARVGGIMANNTYRAQFIYENIQIQNNVIHAAYLNGVKK
                     LLFLGSSCIYPKDSPQPIKEEYLLTSPLEYTNEPYAIAKIAGIKMCESYNLQYGTNFI
                     SAMPTNLYGPGDSYDLAKSHVLPALIRKMYLGKCLSENRWEDLRHDLNKWPVDGVDGE
                     SSKNEIFAVLGKYGISETSITFWGSGKPMREFLHSDDMADASVFLMENVDFDALKGHG
                     EIRNTHINIGTGKDISITNLAGMVKEIAGYHGSIRWNTSMPDGTYRKMLDVGKLHSLG
                     WHHSIELEKGIGQAYTDYANS"
     gene            complement(1787..1921)
                     /locus_tag="PLX53_06935"
     CDS             complement(1787..1921)
                     /locus_tag="PLX53_06935"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOY72854.1"
                     /translation="MDLLVGDASKVDKLLGWKPKVQFEELVEIMVKADWDRLSINGRV
                     "
BASE COUNT          525 a          454 c          383 g          568 t
ORIGIN      
        1 gggtgaatcc tctgtccaac ttcccatagc tgggttgtac ttgccgtaaa ccttagaagt
       61 actcataaaa ataaactgcc caacaccagc cattttggct tgctcagcca aggcaactgg
      121 aaggtgtgta ttcacccttg tgtactcctc caacccaatg tttcctgact ggtgcactat
      181 tgctgctagg tggagaacca catcataccc ttcgaaattc aactcttcgg gtttgtactg
      241 tttagcatca acctcatcta ttgaggagtt cttgctgtac tccctataat tttttccaat
      301 aaagctgttc ttaccaataa tcagaattcg tggcatagtg ggtaatgatt aatatatcct
      361 gagtaattgg ttgcccattg tcgtttagct attggcataa tccgtatagg cctgtccgat
      421 tcccttttcc agttcaatag aatggtgcca ccccagtgaa tgcagcttac ctacatccag
      481 catcttacgg tatgttccat caggcataga ggtattccac ctaatgcttc cgtgataacc
      541 agctatctcc ttaaccatcc ctgccaggtt ggttatagat atatccttac ccgttccaat
      601 gttaatatgg gtattccgta tttcaccgtg gcctttcagg gcatcaaaat caacgttttc
      661 cattaaaaac accgaggcat cggccatatc gtcagagtga aggaattcgc gcatgggctt
      721 accgctgccc caaaaggtaa tagaggtttc ggaaataccg tatttgccta gcacagcaaa
      781 tatctcattt tttgacgatt ctccatcaac cccgtcaacc ggccacttgt taagatcgtg
      841 cctcaggtct tcccagcggt tctcggacaa acatttgcca agatacatct tgcgaataag
      901 cgccggaagc acatggcttt tggccaaatc atagctatcg ccgggaccat acaggttggt
      961 tggcatggct gatatgaaat tagttccgta ttgcaggttg tacgactcac acatcttaat
     1021 tccggcaatt tttgcaatgg catagggctc atttgtatac tccaacggcg aggtaagaag
     1081 atactcctcc tttatgggct ggggggaatc cttaggataa atgcacgagc tgccaagaaa
     1141 cagcaatttt ttaacgccgt taagataggc tgcgtgtatt acattatttt gaatctggat
     1201 attctcataa atgaactgcg ctctataggt gttattggcc attatacctc caacccttgc
     1261 agccgccata aaaacatact caggtttctc agaggcaaaa aaattaaata catcttgttg
     1321 gcaaagaagg tctaattcag catgggtgcg taccacaata ttgtagtagc ccttttgccg
     1381 aagtttgcgc tcgagagcgc taccgaccag ccccctatgg ccggcaatgt aaatttttga
     1441 gttggagtcc attttttttg tgctaaaaag attgaaatag ggttatagga gaaaaaaaga
     1501 aaaaaagtaa gtgtaaaggg taaaaatgtt aggattcaca ttccacggtt cacgaatcta
     1561 ttccacccct tcagttcatt aattaatgaa gatttcacta tttaaggaac ttgcaaccct
     1621 tgtattattg tgccccgttg gggctgaaga caagggtaaa ggataagata aacttgtccg
     1681 cctcgtggcg gattcctcct ttctgtaggt tcatgacagg tattagacat gtttaacttg
     1741 catcttctcg gttaactgct acagctcaca attcaatact cactggctaa actcttccat
     1801 ttatgctaag tctatcccaa tcggccttaa ccataatttc caccagctcc tcgaactgaa
     1861 cttttggctt ccatcccaat aatttatcaa ctttagaggc atcaccaaca agcaaatcca
     1921 cttcggtggg
//
LOCUS       DAOOQX010000413         1926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_74891_length_1926_cov_1.996793, whole genome shotgun sequence.
ACCESSION   DAOOQX010000413 DAOOQX010000000
VERSION     DAOOQX010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74891_length_1926_cov_1.996793"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLX53_06940"
     CDS             complement(<1..427)
                     /locus_tag="PLX53_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72855.1"
                     /translation="MEKFKSDLSGQEFPISERVSGKSIRQPIMELIQKDFPQFSAEEF
                     LSLTELNDYREKYITEYLVRQTGELTELEQKVIDSISDRATLSDKLVEDEAQRTFGQK
                     IADKVAAFGGSWTFIIAFLGFILIWICLNVFWLANRNFDP"
     gene            complement(513..1736)
                     /gene="hydF"
                     /locus_tag="PLX53_06945"
     CDS             complement(513..1736)
                     /gene="hydF"
                     /locus_tag="PLX53_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOY72856.1"
                     /translation="MQRGKESKPHIGIYGRRNNGKSSLINLLAGQNVAIVSDFAGTTT
                     DPVKKSFEITGFGPVILVDTAGIDDSGELGEKRVGKTLETINQVDLAILVVAGNTWGD
                     YEERLIEEFSTSDTPFLVIHNKSDLTPASEEFKRVVGSKPHRGFAEISCLWPNNVDTL
                     VEMIKGAIPESSYQVPTLLGDLISYGDVVLLITPIDVEAPAGRLILPQVQAIRDVLDN
                     DAVAIVLKEREVDDFLRKTRIKPALAVTDSQTFSKADASIPRDIPLTSFSILLARMKG
                     DFASYLKGTPKISQLKNGDRVLLLESCSHHVSCDDIGRTKIPRWLSNFTGKQLEYDVV
                     AGLSSLPRSITDYALVIQCGGCMITRKQLINRIRPAVEAGIPVTNYGMAIAYVQGIYQ
                     RAIAPFAPASPTSYL"
BASE COUNT          493 a          463 c          450 g          520 t
ORIGIN      
        1 aagggtcgaa gttccggttg gccagccaga aaacattcag gcaaatccag attaaaataa
       61 aacccagaaa agcaataatg aaggtccagc taccaccgaa tgcggcaacc ttgtcggcaa
      121 ttttctggcc gaaagtacgt tgagcttcat cctcaaccag cttatccgaa agggttgccc
      181 ggtcggatat ggagtctatc actttttgct caagctcggt gagctcgccg gtttgcctca
      241 ccaggtattc ggtgatgtac ttctcgcggt aatcgttaag ctctgttagc gataggaact
      301 cttcggctga aaactgaggg aaatcctttt gaatcagctc catgatcggc tggcggattg
      361 atttacctga aacccgttca gaaattggaa attcctgacc cgataggtca cttttgaatt
      421 tttccataac ctttcattaa tttgttcgta cctgaaggag atttgaagtt tcggactgct
      481 atattaagct ggggtaaaac gggtaaactc aactataggt agctggttgg actagccgga
      541 gcaaagggcg caatggcacg ctggtatatt ccctgaacat aggcaatggc cataccgtag
      601 ttggtaactg gtattcccgc ttcaacggca ggtcttatcc tgttgataag ctgcttgcgg
      661 gtaatcatgc agccgccaca ctgaataacc agtgcgtaat cggtgatgct gcggggtagg
      721 gagctaagcc ctgccaccac gtcgtactcc agctgcttac cggtaaagtt ggaaagccac
      781 ctggggattt ttgtacggcc aatatcgtcg caggaaacgt ggtggctaca gctttcgaga
      841 agcaacaccc tgtcgccgtt ctttagctgc gaaatttttg gggttccctt caggtagctg
      901 gcaaaatcac ccttcatcct tgcaagcagt atgctgaagc tggtaagggg aatatccctc
      961 gggatggagg catcggcctt ggagaaagtc tggctatcgg taacagccag cgcaggtttg
     1021 atacgggttt ttctgaggaa gtcgtcaacc tcacgctcct tcagcactat ggccaccgca
     1081 tcgttatcaa gcacatccct gatggcctga acctgtggta gtataagcct tccggctgga
     1141 gcctcaacgt caatcggagt gataaggagc accacgtcgc cgtaggatat taaatcaccc
     1201 aaaagggttg gaacctgata cgacgattcg ggaatagccc cctttatcat ctcaaccaac
     1261 gtatccacgt tgttaggcca aaggcagctt atttccgcaa agcccctgtg gggtttggaa
     1321 cccacaaccc gtttgaactc ctcactggca ggggttaaat cggatttatt gtggatgacc
     1381 aaaaaggggg tatcgctggt tgaaaactct tcgatcagcc tttcctcata atcaccccag
     1441 gtgttacctg ccacaaccag aatagccaaa tctacctggt tgattgtttc caatgtttta
     1501 ccaacccgtt tttcccccag ctcgccgcta tcgtcaattc cggcggtatc aaccagaata
     1561 accggtccaa agcccgtaat ctcaaacgat ttcttcacag ggtcggttgt ggttccggca
     1621 aaatcggata ctattgctac attctgacct gcaagcaggt tgataagcga gctttttccg
     1681 ttgttgcgtc ttccataaat gccaatgtgt ggctttgact cttttcctct ctgcatgggc
     1741 tggaatgttt gttggcaaat ttaggcattc gtcggctatt tattcgttcc ttgttaaaca
     1801 ttatgcacaa gcttcatgtc ggtatcaaga atcttaatat caacattaaa gaatgaaaat
     1861 aatttgcatt taacaaaact atcggctaaa ttgcatgtaa ttccttataa ataacatacc
     1921 atgaat
//
LOCUS       DAOOQX010000414         1921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_75200_length_1921_cov_1.972133, whole genome shotgun sequence.
ACCESSION   DAOOQX010000414 DAOOQX010000000
VERSION     DAOOQX010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75200_length_1921_cov_1.972133"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="PLX53_06950"
     CDS             complement(<1..1208)
                     /locus_tag="PLX53_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOY72857.1"
                     /translation="MRISTYIALIATGIIALLPLRSAYPQGIMDSQQVIADIIEDLTS
                     KAETDEDYSQLAEDLLELSENKINLNSAKKDDLLKLIFLSDFQVQSLLDYRDSTGSIL
                     SIYELQAVPGFDLVDVQRLLPFVKVENAEHAVTASELLGGRNEISIRFKTPLETSAGY
                     SDSYSAASRYTGSKDSYYTRYTYSGGKHFQVGLIAEKDAGEPMFDGTFKTGFDYLSGN
                     IVLSDVGRVKKLVVGDYHAEFGQGLTFWNGISFGKSGNVLSGHKRGRGLVRHSSAYES
                     QYLRGVGLTMPVLKADLTIFGSYRHIDAGIADTLDDGDLAFSSLPETGYHRTTSEIAN
                     RNTLQEMVAGANLTYNLRAVKTGLTFFHSEIEGVNAKTLAIYQLEPIRATKNALGMNV
                     DAYLGKHLLFG"
     gene            complement(1211..>1921)
                     /locus_tag="PLX53_06955"
     CDS             complement(1211..>1921)
                     /locus_tag="PLX53_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72858.1"
                     /translation="LNDSWAGFRNQAALAVNRNFWAGIHHENRFLTSELGLSTLGVLI
                     PVSPGTFGVDISHFGYSQLNTTRIGLSYGMKFSEKFMAGIGLAYHRMQVAGEYKDRNA
                     FTVEGGILYAPVEKLTLGAYIFNPTRSSFDDSQSLPPIIGAGATFRPSQKVVLTLQVD
                     DDTESSPNVRGGLEFTATKNFCFRLGYSSGNPEGLTGGFGWMVKDINFDLSFGYHRIL
                     GYTPQLSVSYCPGSKKNS"
BASE COUNT          467 a          479 c          480 g          495 t
ORIGIN      
        1 ccaaacagca ggtgtttacc caaataggca tcaacgttca tacctagagc gttctttgtt
       61 gcgcggatgg gctccagctg gtagatggca agcgttttgg cgttaacccc ctcaatttct
      121 gagtgaaaga atgttaagcc cgttttcact gccctgaggt tgtaggttag gttcgcaccg
      181 gctaccatct cctgtaaggt gtttcggttt gcaatctcgg aggttgttcg gtggtagccg
      241 gtttcgggaa gcgacgaaaa ggccagatca ccatcatcaa gcgtatcggc tatgccggca
      301 tcaatgtggc ggtaggagcc aaaaatggtt aaatcggcct ttagaacagg catggtaaga
      361 ccaactcccc gtagatactg actctcataa gctgacgaat gtcgcaccag cccacgtcca
      421 cgtttatgcc ccgaaagaac attacccgat tttccaaatg agatgccgtt ccaaaaggtg
      481 agtccctgcc caaactcggc gtggtagtcg cccaccacca gttttttaac cctacccaca
      541 tccgaaagga ctatattccc cgagaggtag tcaaaaccgg ttttgaatgt cccatcaaac
      601 atgggttcgc cagcatcctt ctcagctatt aaacctacct gaaagtgctt tcctccgcta
      661 taggtgtaac gggtatagta ggagtctttg ctccccgtgt accgtgatgc tgcggagtat
      721 gaatcggagt acccggccga tgtttccaaa ggcgttttaa accgaatgct gatttcattt
      781 ctaccaccta gcagctcgct ggcagttaca gcatgttcgg cattttcgac ctttacaaaa
      841 ggaagtaatc tttgaacgtc aaccaaatcg aaacccggaa cagcttgtag ctcgtaaatg
      901 ctaagtatgg agccggttga gtccctgtag tccagcaggc tctgtacctg aaaatcgctg
      961 aggaagatta gctttagcag gtcatccttt tttgctgagt tcaggttgat tttgttttcg
     1021 gacagctcaa gcaaatcttc agccagttgg gagtagtcct cgtcggtttc tgcttttgag
     1081 gtaagatcct caataatgtc ggctatcacc tgctgggagt ccatgatacc ctgcgggtat
     1141 gctgacctga ggggaaggag ggcaataatt ccggttgcga ttaaggcaat atatgttgaa
     1201 atcctcatgg ctaggagttc tttttgctgc ccgggcagta gctcaccgat agctgcgggg
     1261 tatagcctaa aatcctgtga tagccaaagg aaagatcaaa atttatgtcc ttaaccatcc
     1321 atccaaaacc accggttagc ccttcagggt ttccagatga gtagccaagc cggaagcaga
     1381 aattcttagt tgccgtgaac tcgagaccgc ccctcacgtt aggactactt tcggtgtcat
     1441 catcaacctg aagggtaagt acaactttct ggctgggtct gaatgttgcc cctgcaccaa
     1501 ttattggcgg cagcgattgg ctatcatcga atgaggatcg ggtagggtta aagatgtagg
     1561 cgccaagggt tagtttctct accggggcat ataggattcc accttcaact gtgaaggcat
     1621 tcctgtcctt gtactcaccc gccacctgca tccggtggta ggcaagtccg attccggcca
     1681 tgaacttctc cgaaaatttc atgccatacg acaagccgat gcgggtggtg tttagctggc
     1741 tgtagccgaa atgcgagatg tcaaccccaa aagtgccagg cgaaacagga atcaggactc
     1801 caagtgtgct cagcccaagc tcgctggtca gaaatctgtt ttcgtggtgg atacctgccc
     1861 agaagttgcg gtttacagct aacgcagctt ggttcctgaa ccctgcccag ctgtcgttca
     1921 g
//
LOCUS       DAOOQX010000415         1917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_75409_length_1917_cov_3.705693, whole genome shotgun sequence.
ACCESSION   DAOOQX010000415 DAOOQX010000000
VERSION     DAOOQX010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75409_length_1917_cov_3.705693"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLX53_06960"
     CDS             complement(<1..712)
                     /locus_tag="PLX53_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOY72859.1"
                     /translation="MSGIDHLIQSDRQFRLQLTSYLSDLVLERRLNLLREVLSRRTRY
                     ITVVLEDLYQPQNASAALRSCECLGIQDVHIIENRNSFNVNPEVDMGASKWLTIKKYS
                     GNKSITQEALQTLRSSGYRTVATSPHSLGCSIRDFDTEKGKFAILFGTELSGLSEEAM
                     SLSEEFITIPMYGFTESYNISVSVAITLYELVHRAKANPAYPLSEYEKEVVLLDWLRK
                     SVKNCHIVEKRFVAEYNKL"
     gene            complement(989..1261)
                     /locus_tag="PLX53_06965"
     CDS             complement(989..1261)
                     /locus_tag="PLX53_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOY72860.1"
                     /translation="MNKAQLIDAIAAEAKVTKADAKKALDAFIKTTGTALKKGDRVAL
                     VGFGSFSVAKRSARTGRNPQTGKPINIKAKKVVKFKAGSELNGKVQ"
     gene            1728..1816
                     /locus_tag="PLX53_06970"
     tRNA            1728..1816
                     /locus_tag="PLX53_06970"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1762..1764,aa:Ser,seq:tga)
BASE COUNT          512 a          395 c          409 g          601 t
ORIGIN      
        1 gcaatttatt atactctgca acaaatcttt tttcgacaat gtggcagttt ttcacggatt
       61 ttcttaacca gtcgagtagc acaacctctt tctcatattc cgacaggggg tatgcggggt
      121 ttgcttttgc ccggtgaacc aattcgtaga gggttatggc aacagaaacc gatatgttgt
      181 agctttcggt gaagccgtac atcggaatgg tgataaattc ttccgaaagg ctcatggcct
      241 cttcggacag gcccgagagt tctgtgccaa aaagaatggc aaattttcct ttttcggtat
      301 cgaaatcgcg aatgctgcac cccagtgaat ggggtgaggt ggcaactgtc cggtagccgc
      361 tactcctcaa tgtttgtaac gcctcttggg ttatgctctt attgccggaa tactttttaa
      421 tggtcagcca ttttgatgcg cccatgtcaa cctcggggtt aacgttaaaa gaattcctgt
      481 tttcgatgat atgaacatcc tgaatgccaa ggcattcaca acttcttaga gctgcgctgg
      541 cgttttgagg ttggtacaaa tcctctagaa ccacagtaat gtagcgtgtt cgtctgctca
      601 aaacctccct gagcaagttt agcctgcgtt caagcaccag gtcagacagg tagctggtta
      661 gctggagacg aaactgccga tcactctgta ttaaatggtc aatgccgctc attccatact
      721 attatcattc ttactaagtg ggttatgcag ttcagctcct ggggtgatta ggtcttgtaa
      781 cagctagcat catccgactg aatagggtta acgcccttca accaggttag ctgatgggta
      841 aaatttaccc cttaagccga atggctacca tgattaattt gcctggctgg aagcataaca
      901 tgtaacaaaa ggctagcttg cacggcttta ccttcaagta aaaaaaaagg agaacactaa
      961 ggttctccct tgggattttc tgtattagct attgaacttt tccattcagt tcgctaccag
     1021 ctttaaactt aacaaccttc ttagccttga tgttgatggg ctttccggtt tgagggttac
     1081 gtcctgtgcg ggcgctcctc ttggcaacgg agaatgagcc aaaacctacc agggcaactc
     1141 tatcaccttt cttaagggcg gtgccagtag ttttgatgaa agcatcaagt gctttctttg
     1201 catcagcctt ggtaacctta gcttcagctg caattgcatc aattaattga gctttgttca
     1261 taatagataa attttagggt taaaaaatag ttgttagttg gctttccatg acaaaattat
     1321 tacataaatc agtaaaagca agttttttca aaaaaaaatt tgtcatttca gccttttttg
     1381 ttggttttta agaaaccaaa atttttacca acgctaaatt attgaatttt taaacaatca
     1441 tgcaactatt tttataagaa aattcgtttt tttttgattt tctccgcaaa atttctaagt
     1501 gaaaaacgac attaagtgtt ataatgtatt atatatcaat aacatacaat ttatttcagg
     1561 tatctacttg attttaaaaa aattatcacc aagttatcaa cagttgctat gttgtaggct
     1621 gtttttacca ctattgcctc aaagttttgc cctaaatatg cctggaatta tatacttttt
     1681 cccttttttg ctttgcagga ataaaaaaag gcggtacttt tgccgccgga gaaatggcag
     1741 agcggtcgaa tgcggcggtc ttgaaaaccg ttgttcgcct tacggcgaac cgggggttcg
     1801 aatccctctt tctccgcaga aggcaagagc atgggaataa gtgcttttgc ctttttttgt
     1861 ttcgggggtt cgaaccgagg agcgccagcg acgagctcgc gagcggagcg agcaatc
//
LOCUS       DAOOQX010000416         1917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_75410_length_1917_cov_3.518260, whole genome shotgun sequence.
ACCESSION   DAOOQX010000416 DAOOQX010000000
VERSION     DAOOQX010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75410_length_1917_cov_3.518260"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1857)
                     /locus_tag="PLX53_06975"
     CDS             complement(<1..1857)
                     /locus_tag="PLX53_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY72861.1"
                     /translation="MKKFFLLLLMQLAAAWAFGNPTDFSGSLKVSGRVTDSEGNPLVG
                     ATVALEGTLLGTSTNPDGSFVLNRLRPGTYVLIASFVGFETARLNLVLQENVVINITL
                     KESTLMFDEVVVSATRASGRMPIAQTTISKEQIDGNNTGFDVPYLLELQPSVVATSEG
                     GTGIGNTAIRIRGTDMSRINVTVNSVPLNDAESQGVFWVDLPDFTASVDNLQVQRGVG
                     TSTNGAGAFGATINFQTVSLSQTPFTSFDIFAGSYNTWKLSARFGTGLVKEKFSFEGR
                     YSQLGSDGYIDRAGSDHRSMFMTAAWHGAKSLIRVNIIHGEEHTGITWEGTPDYMLQI
                     NRRYNPAGEYTDEFGNTRYYKDQKDNYWQTHYQLISTFSLGSNLTLNATVHLTDGKGY
                     YEEFKEDRKFSKYALPNLVLGIDTIRTSDIIQRKWMENQFYGAVASVNYRKKSLEVAV
                     GGGWNRYDGDHFGRILWSAYNYGLPKDYEWYRNNGLKTDWNIFAKSVWQPGKMLSLFA
                     DIQLRGIQYDLKGPDSDLLPVDQEHSWHFVNPKIGTTFRFSPASELYVSFGVANREPS
                     RSDLKDALKGATGIAPKPERLYDWELGYGLRHQDFAINTNIYYMKYRDQLVLT"
BASE COUNT          480 a          461 c          472 g          504 t
ORIGIN      
        1 ggttagcacc agctggtccc tgtacttcat gtagtaaata tttgtgttaa tcgcaaaatc
       61 ctgatgccta aggccatatc ccaactccca gtcgtaaagt ctttcgggtt ttggggcaat
      121 gcctgttgcg ccctttaggg catccttgag gtcgcttcgc gacggttcac ggttggcaac
      181 tccaaatgaa acgtagagtt cgcttgcagg agaaaaccta aatgttgtac caatctttgg
      241 gttaacaaaa tgccagctat gttcctggtc aaccggcaga aggtctgagt ccggaccctt
      301 taggtcatat tgtatgccgc gcagctgaat atcggcaaaa aggctaagca ttttaccggg
      361 ttgccaaacc gatttggcaa agatattcca gtcggttttt aggccgttgt ttcggtacca
      421 ttcgtaatct ttaggtaacc cgtagttata agcactccag agaatccggc caaagtggtc
      481 gccatcgtac cggttccaac caccaccgac agcaacttca agagattttt ttcggtagtt
      541 taccgaggca accgcaccgt agaactggtt ttccatccat ttgcgctgga tgatatcgct
      601 agtgcgaatg gtgtcaattc caagaacgag gttaggcagc gcatattttg aaaactttct
      661 gtcctccttg aactcctcgt agtacccttt tccatccgtc agatgaacgg tagcgttaag
      721 tgttaagtta gaacccaggg agaaggttga aataagctgg tagtgggttt gccagtagtt
      781 gtccttctgg tctttatagt agcgggtatt gccgaattca tcggtgtact caccggcggg
      841 gttgtagcgg cggttaatct gtaacatgta atcgggtgtg ccctcccatg ttattcccgt
      901 gtgctcctcg ccatggatga tgttaaccct aatcagactt ttagcgccgt gccatgctgc
      961 ggtcatgaac attgaccggt ggtcggagcc cgcgcgatca atatatccat cggagccaag
     1021 ttgcgagtaa cgaccctcaa agctaaattt ttcttttacc aaacccgttc cgaaccgagc
     1081 agagagtttc caggtgttgt aggagcccgc aaaaatgtcg aatgaggtaa atggtgtttg
     1141 cgatagggaa accgtttgaa agttgatagt tgctccaaat gccccggcac cgtttgttga
     1201 agtacccacc ccgcgttgca cctgaaggtt atctaccgaa gcagtaaagt cgggcaaatc
     1261 gacccaaaaa acgccctgcg attcggcatc gttcaggggc acgctgttaa ccgtaacgtt
     1321 tattcgcgac atatcagttc ctcgaatgcg aatagccgtg ttgccaatgc cagtcccccc
     1381 ctcggaggtt gcaacaacgg agggttgaag ttccaggaga taaggaacat caaaaccagt
     1441 gttgttcccg tcaatttgct ccttgcttat ggtagtttgg gcaattggca tacgtccgga
     1501 agcccgagta gcgcttacta caacctcgtc gaacatcagc gttgactcct taagagtaat
     1561 gttgataact acattttctt gcagcaccag gttgagcctg gcggtttcga aaccgacaaa
     1621 tgaggcgatc agcacatagg ttccgggtcg caagcggtta aggacaaagc tgccatcggg
     1681 gttggttgag gttcccagca gggtaccctc aagagctacg gttgctccaa ctagcggatt
     1741 accttcagaa tcggtaaccc taccgctaac ttttaatgag cctgaaaaat cggttggatt
     1801 accaaaagcc catgcagctg caagctgcat gagcaacaaa agaaaaaatt ttttcatggt
     1861 ttttaccctt ttttaagtgt taaacatcgg gccaccaaaa gcaatagcgg tgtgcac
//
LOCUS       DAOOQX010000417         1916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_75463_length_1916_cov_2.951102, whole genome shotgun sequence.
ACCESSION   DAOOQX010000417 DAOOQX010000000
VERSION     DAOOQX010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75463_length_1916_cov_2.951102"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..>1916)
                     /locus_tag="PLX53_06980"
     CDS             complement(37..>1916)
                     /locus_tag="PLX53_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOY72862.1"
                     /translation="SSYYHIKSTKREFIIEPYWFTYTGTNMKVLETSICVPLVDNDRF
                     VALFGFDIELERYHEFIDSVEIFPGSNSMLISQDGLIVAHSDSSLTGIRVDSIFNQQK
                     IGFNIIDKLKKESDFSFNVNMNGSEYYASFQSFAISGTTNRWTFASLSPLSIIVEESK
                     DVATRSRIVILAGLLILALVVWIISYSITHPLVKATRTLRELAGGEIDTSKKLAINSG
                     DEVEDIGSSINILIDSLYKTTNFAREIGKGNFNVDYSKLSENDKLGEALLDMRKSLEH
                     ARLQEVERKLGEDKNRWANEGVAKFAEILRQNSDDLNEFCYSFIYNLVKYVDANIGAI
                     YLVNNDNPNEVYFELMATYAYERRKYEEKRIDFGEGLVGRCAKEAETIYMTDLPKDYI
                     KISSGLGEESPTCLLLVPIKLNENVHGVIEMASFEPIEDYKIRFIEKIGESLAATISN
                     VKINIRTGKLLDESRVKSEELASQEEEMRQNMEELQATQEEAARKTAEMESLIGALHA
                     SSFVIEYDLNGNITSVNQAYLTLTGQSENEVIGTHHSDNLEMTDEQKKNYQKFWNDLR
                     NGMVKKETSRIKLGKKTLTFIETYSPIFNQNRQVVKILKIAHNITDFIEDEKVKGRKT
                     K"
BASE COUNT          497 a          419 c          351 g          649 t
ORIGIN      
        1 aaaaaagaga gtcgctgata agcgactctc ttttttttat ttcgtttttc gacctttaac
       61 tttctcgtcc tctataaagt cggttatgtt gtgtgcaatc ttcaggattt taacaacctg
      121 ccggttttgg ttgaagattg gcgagtaggt ttcaataaag gtaagcgttt tctttccaag
      181 cttaatacgg ctggtttcct ttttaaccat cccgttacgc aaatcgttcc agaacttctg
      241 atagtttttc ttttgctcat cagtcatttc cagattatca gaatggtggg tgccaataac
      301 ttcattctct gactgtccag ttagtgtaag gtaagcctgg tttacagaag taatattgcc
      361 gttcaaatca tactcaatta caaagcttga ggcatgtaat gctccgatga ggctttccat
      421 ttcggctgtt tttctagctg cttcctcctg ggtggcttga agttcctcca tgttttgcct
      481 catttcctcc tcctgagaag ccagctcttc tgatttaacc cgggactcat ccagcaactt
      541 tcctgtacgg atgttgattt ttacatttga tattgttgca gccaagcttt ctccaatctt
      601 ctctataaac ctgattttat aatcctctat aggctcaaat gaagccattt caataacccc
      661 atggacattt tcatttagct ttattggcac tagaaggaga catgtagggg attcctcccc
      721 aagtccggag gaaattttta tgtaatcttt tggcaggtcg gtcatgtaaa tggtttcggc
      781 ttccttagcg catcttccaa ctaacccttc accgaaatct attctttttt cctcgtactt
      841 cctacgctcg tatgcatagg tagccattag ctcgaagtaa acctcattag ggttgtcatt
      901 atttaccagg tatattgcac ctatgttagc atcaacatac ttaacaaggt tgtagataaa
      961 cgagtagcaa aattcgttca gatcatcgct attttgacga agtatctcgg caaatttagc
     1021 cacgccttca tttgcccatc tgttcttatc ttcacctagc ttacgctcaa cttcctgtag
     1081 tctggcatgc tccagacttt tcctcatatc tagtagggct tcaccaagct tatcgttttc
     1141 gcttaacttg ctgtagtcaa cattaaaatt gcctttccct atttcccttg caaagttggt
     1201 tgttttatat aggctatcaa ttagaatatt aattgagctt cctatatcct caacctcatc
     1261 tccagagttg atagctagct ttttactcgt gtctatttca cctccagcaa gttctctgag
     1321 ggttctggtg gcttttacca atgggtgggt aatgctgtag gaaataatcc atacaaccag
     1381 tgcaagaatc agcaacccgg ctaatatgac tattctggag cgtgttgcta catccttcga
     1441 ctcttcaaca ataatggata gaggtgaaag tgaggcgaag gtccaccggt ttgttgttcc
     1501 actaattgca aatgattgaa aagatgcata gtattcactc ccattcatgt taacgttaaa
     1561 ggagaaatca gattcctttt taagcttatc tatgatgtta aagccaattt tttgttggtt
     1621 aaaaatgcta tctacacgaa tcccggtcag acttgaatcg ctatgcgcaa ctatcaaacc
     1681 atcttgggaa atcaacatcg aattgctacc agggaatatt tcaacgctgt caataaattc
     1741 atggtaacgt tcaagctcaa tatcaaatcc aaatagagca acgaaccggt cattgtcaac
     1801 cagcggtaca caaatgctgg tttcgagcac tttcatatta gtcccagtat aggtaaacca
     1861 gtacggttca attataaatt ctcttttggt tgacttaatg tggtagtatg agctgt
//
LOCUS       DAOOQX010000418         1904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_76198_length_1904_cov_3.178475, whole genome shotgun sequence.
ACCESSION   DAOOQX010000418 DAOOQX010000000
VERSION     DAOOQX010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76198_length_1904_cov_3.178475"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PLX53_06985"
     CDS             <1..752
                     /locus_tag="PLX53_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HOY72863.1"
                     /translation="AGKNLVFILSQKEKIELLSKTGLDHLIIVRFTLEFSHISSVDFI
                     QHFLIAKLHARFVVVGFNHHFGHNREGDFEELKRLGLQYNFGVEEIPEQDIQQETVSS
                     TAIRKALLEGKIQRANAYLDHHFIIMGTLGKGAHIFQRAGFPTLTVQIEEAGKLIPHE
                     GIYAVTLEWNSTHYRAMAIIWLDNYGSNEPVLGPLNVDVHIMEFDNILSEDDAKLFFQ
                     KQLADHVDIADEERLTSQLSAACSSLDELIF"
     gene            925..1170
                     /locus_tag="PLX53_06990"
     CDS             925..1170
                     /locus_tag="PLX53_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOY72864.1"
                     /translation="MERRIGAALILVENRGSVEKLNQLFSAHSSVIVARQGIPLQQRG
                     VSVISLVLDGTSDEISALTGPVGKLDGVQVKTLMMKG"
     gene            1402..1878
                     /locus_tag="PLX53_06995"
     CDS             1402..1878
                     /locus_tag="PLX53_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY72865.1"
                     /translation="MKFNPEVYSIPDERMKPFIDPDEIWGYINSAKPTRERVREIIAK
                     SLDKHRLNLEEVAVLVNTSEPELVEEILQGARTLKQKVYGNRIVLFAPLYIGNKCTNN
                     CKYCGFRTSNTEAVRVTLSDEEITKEVEALEDNGQKRLILVYGEHPEYSADYIAHQ"
BASE COUNT          548 a          400 c          445 g          511 t
ORIGIN      
        1 atgctggtaa gaatctggtc ttcatacttt cacaaaagga aaaaattgaa cttttaagca
       61 aaacaggtct tgatcacctg attatcgtca ggtttaccct ggagttttcc catatctcgt
      121 cggttgactt tatacagcat tttttaatag caaagcttca cgccaggttc gtagttgtcg
      181 ggtttaacca ccattttggc cataaccgcg agggagattt tgaagagctg aaacgccttg
      241 gcctgcagta caactttgga gtagaggaaa ttccagagca ggatattcag caggaaaccg
      301 ttagctccac tgcaatccgt aaggctttgc tcgagggtaa aattcaacgg gccaacgcct
      361 acctcgacca tcactttatt attatgggta ctttgggcaa aggtgctcat attttccaga
      421 gagcaggatt ccccaccctt accgttcaaa ttgaggaagc aggaaagctg ataccccacg
      481 agggtattta cgctgtcacc ctcgaatgga atagcaccca ttaccgcgcc atggccatta
      541 tttggctcga caattacggc agtaatgaac ccgttttagg gccactcaac gttgacgttc
      601 atataatgga gtttgataat atcctttccg aggatgatgc aaagttgttt tttcaaaaac
      661 aacttgccga tcatgttgac atagctgatg aggaacgcct gactagtcag ctttcggcag
      721 cttgcagctc cttggatgag ctcatttttt aaggagcagc tccgataaat tttaaagctt
      781 tttgtgctga agcatagcgt aactatttgt aactttacga cgtaattaaa cttgtatttc
      841 aaggttaaaa atctgtatcc gggaaaggaa ttttaattgc tttcgaggat tcagctaaca
      901 gttctaatcc aaaggagaac tctgatggaa aggagaatcg gggcggcgct tatcctggtg
      961 gagaaccggg gaagcgtgga gaagttaaac cagctatttt cggcccattc cagcgtgatt
     1021 gtcgcccggc aggggattcc gcttcagcag cggggtgtta gtgttatctc gttggtgctc
     1081 gatggcacca gtgatgaaat tagcgcacta accggtccgg taggcaagct cgatggcgta
     1141 caggtaaaaa ccctcatgat gaaagggtaa ttctacctat tttctctcct ttacattaga
     1201 attgcgctat tctaacctat cattaggttg gagattatgg gtatgcaaag catattcatt
     1261 cccatgaagt atatatgtta tggggaagat ttagtacgaa gcgtgaaaaa aatgatgaat
     1321 tacttaaaat tatgaaatga ggggcattgc aactctttgc atgcattacc aactaaatat
     1381 taacaattaa tttgcaaaca aatgaaattt aatcctgaag tttacagcat tcccgatgag
     1441 cgaatgaaac ctttcatcga tcccgatgaa atttggggtt acatcaacag cgccaagccc
     1501 acccgcgaaa gggtgaggga aatcattgcc aaatcgctcg acaagcatcg gctaaacctt
     1561 gaggaggttg cagttctggt aaatacctcc gagcctgagc ttgttgagga aatcctgcag
     1621 ggagctcgta cacttaaaca aaaggtttac ggcaatagaa ttgtcctatt tgcgccgctt
     1681 tacatcggga ataagtgcac aaacaactgt aagtattgcg ggttcagaac ctccaacacc
     1741 gaagctgtga gggtaaccct ttcggatgag gaaattacca aggaggttga ggcgcttgag
     1801 gataacggtc agaaaaggct gatactggtt tacggagaac acccggagta ctccgccgac
     1861 tacattgccc accaatgaaa atggtattgg cgacaaccca ttgg
//
LOCUS       DAOOQX010000419         1899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_76473_length_1899_cov_3.554230, whole genome shotgun sequence.
ACCESSION   DAOOQX010000419 DAOOQX010000000
VERSION     DAOOQX010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76473_length_1899_cov_3.554230"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(338..1726)
                     /locus_tag="PLX53_07000"
     CDS             complement(338..1726)
                     /locus_tag="PLX53_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOY72866.1"
                     /translation="MAYRFARLLFAGLFFALPLAGYPQLTAPGRSWAGVTQYVNSPLH
                     QDSIFVFFSNVGSLRASHSTGNNASFKWYRYDPLAPSISERFVLFSTPSDGFFSQLDN
                     LAEGGYRVEVTDDADSTETFTAWLFTDNVTLTGIDVFSQCSFLQLTAISSPDQYAVEY
                     DRFVYYDLSRTSHTVNNKYGKDYFKVISWNASEPRVEVPDIQQLKLTLENPAPLWNSA
                     YTVTITNCFGRQLPVASTDVVTGIAAKSDMKIQVDANGVWTDWSSNERYEALLGLRLE
                     SLSVNVDSLYWQVVNEKIGKFANEYFVIWRDSSTVEQRKEAFPDKDLMAPGYYLVKHF
                     VINTTSGCRDSMVVNIEVDSSQIKADALPNVFSPNDDGVNDVFSFINPEENIKSIRTF
                     TIRILSRTGKQIYSYSGDPREWKGWDGRIEGSGAKAATGVYYFIIEARGWDDRTFKRG
                     PYKGFLHLYADR"
BASE COUNT          540 a          449 c          463 g          447 t
ORIGIN      
        1 gcgaacgatt tttacaagcc ctgaaggggc ggaataggtt tgaaacgaag aaccttgaga
       61 gtttggaacc aagaaccccg aaggggcgta atacctagcc cagggtaaag ccccgggttt
      121 aattgtggcc tcggaagtac tagcgatgca agtagcggct tttccaaaga gctgtacgga
      181 ttctttgcat cgcaacggga agaaataagt ataaagcgta aagggtaaaa aaaattccaa
      241 aacttgtttg cctgttgaag aattagccct taaccaggct ttggaatcga aaaacatctc
      301 caattttaaa agggtaacag ggaaaaacca tgaaaattta cctgtcggcg tagaggtgta
      361 gaaaaccctt gtaggggcca cgcttaaagg tgcggtcgtc ccagccccta gcctcaataa
      421 tgaagtagta aacccccgtg gcagccttgg ctccgctccc ctcaatgcgt ccgtcccagc
      481 ccttccactc gcgcgggtcg ccactgtagg agtatatctg cttgccggtt cggctaagta
      541 ttctaatggt aaacgtccgg attgacttga tgttctcctc ggggttaatg aaggaaaaca
      601 cgtcgtttac cccgtcatca ttgggtgaaa atacattggg cagggcatcg gcttttatct
      661 gcgaggagtc aacctcgatg ttcaccacca tggagtcgcg gcatccggaa gtggtgttga
      721 tcacaaaatg ctttaccagg tagtagcctg gcgccatcaa gtccttatcg ggaaaagctt
      781 cctttctctg ctccaccgtg gagctatcgc gccaaataac gaaatactcg ttggcaaact
      841 tgccaatttt ttcgttaacc acctgccagt aaagggagtc gacgtttacc gaaagactct
      901 ccaggcggag tcccaataga gcctcatacc tttcgttgct gctccaatcg gtccacacgc
      961 cattggcatc cacctgaatt ttcatgtcgg atttggcggc aatgcccgtc accacatcag
     1021 tagaagcaac cggtagctgc cttccgaagc agtttgtaat ggttaccgtg taagctgaat
     1081 tccaaagggg ggcaggattc tcgagggtaa gcttaagctg ctggatatcg ggaacctcca
     1141 ccctcggttc ggaggcattc cacgaaatca ccttaaagta gtcctttcca tacttattgt
     1201 ttaccgtatg agaggtacgc gaaaggtcgt agtacacaaa cctgtcgtac tcaacggcat
     1261 actggtcggg gctcgatatg gcagttagct gcaggaagct gcactggcta aaaacgtcaa
     1321 tgccggttag tgttacatta tcagtaaaaa gccatgctgt aaaagtctca gttgaatcgg
     1381 catcatcggt aacctcaacc ctataacctc cctcggctaa gttgtcgagc tgagaaaaaa
     1441 aaccatcgga gggagtgctg aagagcacaa atcgttcgct tatggaaggc gccagagggt
     1501 cataccggta ccatttaaat gaagcattat tgcctgtaga atgggacgcc ctaagcgagc
     1561 caacatttga aaaaaataca aaaattgagt cctgatgtaa tggtgagttc acatactgcg
     1621 taacgcctgc ccagctacga cctggagcgg taagctgtgg gtaaccggcc aggggtaatg
     1681 caaaaaacaa ccccgcaaaa agtaaccttg caaatcggta agccattgtg caaacgtaaa
     1741 attagctaca atattaacga ataaaaagta aattagtaat cccagtgcct gaatcgttaa
     1801 atatgagccc tatagtcgaa tcgtgaaaag gtagagtaga aggttaacag ctttatactt
     1861 gcccggcacg tgtcagggga acctgtctta aacccgaca
//
LOCUS       DAOOQX010000420         1899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_76481_length_1899_cov_3.018438, whole genome shotgun sequence.
ACCESSION   DAOOQX010000420 DAOOQX010000000
VERSION     DAOOQX010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76481_length_1899_cov_3.018438"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /gene="secF"
                     /locus_tag="PLX53_07005"
     CDS             <1..454
                     /gene="secF"
                     /locus_tag="PLX53_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOY72867.1"
                     /translation="STGAIVALFHDTMMIVGMYSLLYFRVPFGLEADQSFIAALLTII
                     GYSINDTVVIFDRIREYNKLYPKRSSRDNINNAINDTLSRTFVTSFTVLIVLVPMFFL
                     GGEVIRGFIFALIIGTISGSYSTVFIATPIAYDLEKAVEKRKNRKIKQ"
     gene            699..1688
                     /gene="trpS"
                     /locus_tag="PLX53_07010"
     CDS             699..1688
                     /gene="trpS"
                     /locus_tag="PLX53_07010"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOY72868.1"
                     /translation="MEIVLSGIRSTGKLHLGNYYGALLNFVQMQHEHKCFFFIADYHS
                     LTTHPTPTDLHGSVKQVLAEYLACGLDPEIATLYVQSDLPQVPELYLLLNMNAYVGEL
                     ERCTSFKEKIRKHPDNINAGLLTYPTLMAADILIHKATMVPVGKDQEQHLEMTRTFAN
                     RFNRLYKVDYFPEPVAYNFGKELVKIPGLDGSGKMGKSEGNAIYLSDDPKTIRSKVMK
                     AVTDSGPTQPNQEKSEPVKNLFTLMRVVSDPETYSYFEEQYSSCHIRYGDMKKQLAED
                     IIRITTPIRERIEEILANDAYLSQVAHHGAEKARESASKTINEVRQIIGFRNF"
BASE COUNT          586 a          464 c          391 g          458 t
ORIGIN      
        1 cagcaccggc gctattgtag cgcttttcca cgataccatg atgattgtgg gaatgtactc
       61 gctgctgtac ttcagggtgc cctttgggct cgaggccgac cagtcgttta ttgccgccct
      121 gctaaccatt attgggtact ccatcaacga caccgttgta atcttcgacc gtatcaggga
      181 gtacaacaag ctctacccca agcgttcatc acgcgataac atcaacaacg ctatcaacga
      241 caccctcagc cgaacctttg taacctcctt caccgtgctt attgttttag ttcccatgtt
      301 cttccttggt ggcgaggtta tcaggggctt tatctttgcc ttaatcattg gtactataag
      361 cgggtcatac tctaccgtat tcattgcaac acctattgcc tacgaccttg aaaaagctgt
      421 tgaaaaacgt aaaaacagga aaattaaaca gtaaaatccc gtagcaataa ttaggagcat
      481 tgttaaaaac tcaacccacg cagggttgag ttttttttta gtagaggtta aagtaaataa
      541 aatccgtagc agccacaaat caacccaaag taattgcttc aatatcaaaa tactacctta
      601 tatcaacatt gctgcatcgc tccctgccat tcaacatgga aattgtacgg cataaagttg
      661 tatttttgta gttaaacata acctacgcac cacaagccat ggaaattgta cttagcggaa
      721 tacgctcaac aggcaagctt catttgggaa actactatgg agctttgcta aactttgttc
      781 aaatgcaaca cgagcacaag tgcttctttt ttattgccga ctaccactcg cttaccactc
      841 acccaacacc cacagatttg catggatcgg ttaagcaggt tttagccgag tacctggcat
      901 gtgggcttga ccccgaaatt gccacgctat acgtgcaaag cgatttgccc caggttccgg
      961 agctctacct tttactcaac atgaacgctt acgtgggaga gttggagcga tgcacttcgt
     1021 tcaaggaaaa aatacgtaaa caccccgaca acataaatgc cgggctgctc acctatccca
     1081 cgcttatggc cgccgacatt cttatccaca aggctaccat ggttccggtg ggaaaggacc
     1141 aggagcaaca cctggagatg acccgcacat ttgccaaccg atttaacagg ctctacaagg
     1201 tggattactt ccccgaacca gtggcataca actttggaaa ggagctcgta aaaataccgg
     1261 ggcttgatgg ttcgggtaaa atggggaaat cggaaggtaa tgccatatac ctgtccgacg
     1321 acccaaagac catacgtagc aaggtgatga aagcagttac cgattcggga cccacccaac
     1381 caaaccagga aaaatcggag ccggtaaaaa acctttttac cctcatgcgg gtagtttccg
     1441 atcctgaaac gtacagctac tttgaggagc aatacagctc atgccatatt cgctacgggg
     1501 atatgaaaaa acagctggca gaggatatca tacggattac cacgccaata agggaaagaa
     1561 ttgaggaaat tctggcaaac gatgcctacc taagccaggt agcacaccat ggagcagaga
     1621 aagcgcgtga gagcgcatcc aaaaccatta acgaggtaag gcagattatt ggtttccgaa
     1681 atttttagga aacgatttgc gagagcaaca tgaacagtta ggtggtaaaa tcaatccacc
     1741 caacaaaaaa acatcctaaa acctggcaag ccaaccatag cagacaagca gaaaacatcc
     1801 tactattttc aagcggaaaa gccccattca aaaataattg taccagcagg atgttatatt
     1861 aacacgatta attttataaa tttgttcgtt cacccaaaa
//
LOCUS       DAOOQX010000421         1897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_76581_length_1897_cov_3.331705, whole genome shotgun sequence.
ACCESSION   DAOOQX010000421 DAOOQX010000000
VERSION     DAOOQX010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76581_length_1897_cov_3.331705"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="PLX53_07015"
     CDS             <1..1535
                     /locus_tag="PLX53_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOY72869.1"
                     /translation="SAEATDLLLVPPDEISVTSAADDKVAANSRRLAVEDSLRGCYIA
                     TFIDSLSAVKFAASMGINAEKAWHFLSKSRGNWPEIQNFITNMEPGETPVGMALLGIL
                     AEKDLHDITAATLRSHLKTVDSFPSLLDSRSFNLFDRYILSPRVGREFITPWREFLQG
                     YFTFDQIGFFRNDPTNLVKWISENIKIDSVENYYKVPISPEGVVRLGIADAYSRDILF
                     VAACRSFGIPARLEPSTKRPQYLMRGSWHDVDFGEREVETSGWGTLTLSATSGMEIAS
                     PSYYKHYTIARFENNRFVTLDFENDAQLSTLPFSLSLREGLYRLVCGTRDQNGAVSCR
                     VKYFSIYPDEKTFVTLEFPSSKGKTVVLGNLGDPLIPDIEGSYIKNLGEIGGGGSTVV
                     AIIDPMKEPTRHLVNDLAAIKTQLDKWGGAVVLAIAKDKLPSGFNAQKLLEGVPLPIG
                     VTIGYDSQQSLANALAIACGKPLSTDYPVVAVVNGKGEITFFSEGYSIGLGEKILKEV
                     GE"
BASE COUNT          482 a          417 c          476 g          522 t
ORIGIN      
        1 cttccgcaga agctacagac ctgttgctgg ttccacccga cgaaatttcg gttacgtctg
       61 ctgccgatga taaggttgct gcaaacagcc gccggctagc tgttgaggat agccttcgag
      121 gatgctacat tgctactttt attgattccc tgtcagctgt taagtttgca gcaagtatgg
      181 gtattaacgc cgaaaaggca tggcatttcc tctctaaaag tcgggggaac tggccggaaa
      241 ttcaaaattt tattaccaac atggagccgg gtgaaacacc tgtgggcatg gctctacttg
      301 gaattctggc ggagaaggac ttgcacgaca ttaccgctgc tactttaaga agccatctta
      361 aaactgttga ctccttcccc tcattgctcg atagccgtag cttcaattta tttgaccggt
      421 acattctttc ccccagggtt ggaagggagt tcatcacacc ctggcgggag tttttgcagg
      481 gctatttcac cttcgaccag ataggttttt tccggaacga tccaaccaac ctggtcaagt
      541 ggatttcaga gaacatcaaa attgattcgg ttgaaaacta ctataaggtt ccaattagcc
      601 ccgagggtgt agttaggtta ggcatagccg atgcctactc ccgcgatatc ctctttgtag
      661 cggcttgccg cagttttggc attcctgcgc ggcttgaacc ttctaccaaa cgtccgcagt
      721 acctcatgcg cggcagctgg catgatgttg attttggtga gagggaagtt gaaacttccg
      781 gctggggaac acttacactt tctgctacct ccggcatgga gattgcttca ccgtcgtact
      841 ataagcatta caccatagcc cgatttgaaa acaacaggtt tgttaccctt gattttgaga
      901 acgatgccca gcttagtacc ctgcctttta gcctatcact aagggaagga ctttacaggt
      961 tggtttgcgg cactagagat caaaacggag cggtgagctg tagggtaaag tatttcagca
     1021 tttatccaga tgaaaaaacc ttcgtaacgc tggaattccc atcgtcaaaa ggtaaaacgg
     1081 ttgtccttgg aaatttaggc gacccgctta ttcccgatat tgaaggaagc tatatcaaga
     1141 atctgggcga gattggagga ggagggtcaa cggtagttgc cattatagat ccaatgaagg
     1201 aacctaccag acatttggta aatgaccttg cggccatcaa aacgcagctc gacaaatggg
     1261 gcggtgctgt tgtactagct attgctaagg ataagctacc atctggcttc aacgctcaaa
     1321 agcttttaga gggggtgccc ttgcccattg gcgttactat tggatatgat tcccagcaaa
     1381 gtttggctaa tgcgcttgct attgcttgtg ggaaacccct ttcaaccgat taccctgtgg
     1441 tagcggtggt gaatggcaag ggggagataa cgtttttctc cgaggggtat agcattggac
     1501 tgggtgagaa aattttgaag gaggtggggg agtagtcctg ttgagccttg tccttagcag
     1561 ccccgtaaaa tattttttcc ccaaggaaga cgccaggata aaatttccct tgtttggaaa
     1621 agcacttcag cacctttgcg gttgggtatt tttcatccgg ggagatgcga aggtgcgaag
     1681 gcgacatgtc ggcattctcc tgtacatcat aataaaaccg gcattgaatg ttaagcgaaa
     1741 tgctcatttt agttatcttt gctagctaaa tttagcccaa ccaatctggt tttctatgga
     1801 ttataacttt aaggcaattg aggagaagtg gcaggcgctt tggcagcagt ggaataccta
     1861 tagggtagat gttaacccca acaggccaaa attttat
//
LOCUS       DAOOQX010000422         1893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_76821_length_1893_cov_3.188248, whole genome shotgun sequence.
ACCESSION   DAOOQX010000422 DAOOQX010000000
VERSION     DAOOQX010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76821_length_1893_cov_3.188248"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1861)
                     /locus_tag="PLX53_07020"
     CDS             complement(<1..1861)
                     /locus_tag="PLX53_07020"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72870.1"
                     /translation="MSGGSKSWATSLSQNYGTALTGAPDTLFEDNFEIPDGWTLTGEF
                     ERNTIIMGWDTIPTFAYSGIYCLGTDLSRQGAHKGMYEPNSSYEALSPAVDISSYANL
                     QLAYYRWFKVMEGDTAKVMASNNGLTWTTIWNSAGQEIADENWTEEIIDIPASLVNGS
                     SLTIKFVLKANGDENIAEGINIDDFRIIGNHFSTGMAYLQSPCFNFTGISIPVMDMSI
                     FNRTEAGVDGTTLYYSTDKGETWTPVDNNDTLDDYWNFYTDSLVSSIGLDGWNGLGSG
                     WENTRHLLPAALANNSSVIFRLGFKADKTNNDFGGTAIDNFKLLQAPFDVGVESILSP
                     STTCDLSQNQSIQVRLKNFGIQDMEAGDTIIVGIDIDHPMVPDSFTDTLVLSSTLAVN
                     SHLDYTISKTFNMSVSGDYQVTAFTKIENNPVFYHPEPNDTASTLITVQKPYVELGND
                     IYSYNPENIVLDATNPDPTVTYKWYKSPDLTTVIGTNPTYAIADHDGGKYLVTLTNSI
                     PCSSTDSVTVNRIIRDVGISTLVSPTSSCELSDETPLSVYVKNYGSDTLMSSDDEIYL
                     HYTFNGNPQVDTTWTITQNLLPGDSLLFTFNQTFDMQAVGSYPLHVWANTTLDE"
BASE COUNT          444 a          414 c          517 g          518 t
ORIGIN      
        1 tttcatctag tgtggtgttt gcccaaacat gtaacgggta agaaccaaca gcctgcatat
       61 cgaaagtttg gttgaaggtg aatagtagcg aatcgccggg aagcaggttt tgggtaatgg
      121 tccaggtggt atccacctgg gggttgccgt taaaagtgta atgaaggtat atttcgtcgt
      181 cgctactcat gagcgtatcg gatccgtaat tctttacgta aacgctcagg ggcgtttcat
      241 cggaaagctc acaagagcta gttgggctaa ccagggttga aatccccaca tcgcgtatga
      301 tacggttaac ggtaaccgag tcggttgagg agcagggaat agaatttgta agcgtaacaa
      361 ggtattttcc tccatcatgg tcggcgatgg cgtaggttgg attggtacca ataactgtag
      421 tcaggtccgg cgatttgtac catttatagg ttacggtggg gtcggggttg gtggcatcca
      481 gcacaatgtt ttcggggttg taggagtaaa tatcgttgcc cagctcaaca tagggtttct
      541 gcacggtaat taaagtagaa gcagtatcgt tgggctccgg gtggtagaat actgggttgt
      601 tttcaatctt ggtaaaagct gtaacctggt aatcgccgct aaccgacatg ttgaaggttt
      661 tggaaatggt gtagtccagg tggctgttca cagccagcgt ggaactcagc accagggtgt
      721 cggtgaagga gtcgggaacc atgggatggt caatatcaat gcccacgatt atggtatcgc
      781 cagcctccat gtcctgaatg ccgaagtttt tcaacctaac ctgaatggat tggttctggc
      841 ttaaatcgca ggtggtggaa gggcttaata tgctttctac acccacatca aatggtgcct
      901 ggagtagttt aaagttatca atggcagttc ctccaaaatc gttattggtt ttatcggctt
      961 tgaagcctaa cctgaaaatt acgctactat tattagccag agcagcagga agcaggtggc
     1021 gggtgttctc ccaaccactt cccaacccgt tccatccatc aaggccgatg gatgaaacca
     1081 ggctatcggt gtagaaattc cagtaatcat ccagggtatc gttgttgtca acaggagtcc
     1141 acgtttcgcc cttatcggtg gagtagtaaa gtgtggtgcc gtcaactccg gcttcagtgc
     1201 ggttaaaaat actcatgtcc atcacgggta tggagatacc ggtaaagttg aagcatgggc
     1261 tttgcaggta ggccattccg gttgaaaaat ggttgccgat aattctaaag tcatcaatat
     1321 ttatcccctc cgctatattc tcatcgccat ttgctttgag gacgaatttg atggtaaggc
     1381 tgctgccatt aacaagcgag gcgggtatat caataatttc ttcggtccaa ttttcgtcgg
     1441 ctatttcctg tccggcgctg ttccagatgg tggtccaggt aaggccgttg ttgctggcca
     1501 taactttggc cgtatcgccc tccattactt taaaccaacg atagtaggcc agctgcaggt
     1561 tggcgtagct gctgatgtca acagccgggc tcaatgcctc gtagctggaa ttgggttcat
     1621 acatgccctt gtgggcaccc tgtctgctaa ggtcggttcc gaggcagtat atgccgctat
     1681 aagcaaaggt tggaatggtg tcccaaccca tgattatggt gttccgttcg aactcgcccg
     1741 tcagcgtcca gccgtcgggg atttcgaagt tgtcctcaaa tagcgtgtcg ggtgcaccgg
     1801 tgagcgctgt cccgtagttt tgagaaaggc tggttgccca cgattttgaa ccgccgctca
     1861 tagccggttt ggaaatgctg ccccactgcc ata
//
LOCUS       DAOOQX010000423         1889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_77055_length_1889_cov_4.648855, whole genome shotgun sequence.
ACCESSION   DAOOQX010000423 DAOOQX010000000
VERSION     DAOOQX010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77055_length_1889_cov_4.648855"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..919
                     /locus_tag="PLX53_07025"
     CDS             347..919
                     /locus_tag="PLX53_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72871.1"
                     /translation="MDVETRTTAGQVMGVIGIVLGVVSLVLAFIPCIGVMAFIPGVLA
                     LTFSIISIVQASNGYGSKGLGIGALIVSLVSIVLAAAWLIVFSGTFAFADRAIRNSDK
                     IEIFGKEFGKAFEEEFGKGDVNLKIVSDSLENSLRQLENELDSLDGYIPEEKARKAGQ
                     AAARALKKSTRVVIETDSGKIQFETSNKSH"
     gene            925..1356
                     /locus_tag="PLX53_07030"
     CDS             925..1356
                     /locus_tag="PLX53_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72872.1"
                     /translation="MKPPSCKKGINVYASVNALFIVVILAVFMYSFAFKTEKYPIPAL
                     LTQSTGLIPPSRGLSKSFSEIVRGNISAAKLINPHSLQIFAFFLIQLVMRVAGFAAAK
                     IIGSPFPVKGIAVDVCISLALFVFCFAPLISYTFRLFVQLF"
     gene            complement(1336..>1889)
                     /locus_tag="PLX53_07035"
     CDS             complement(1336..>1889)
                     /locus_tag="PLX53_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72873.1"
                     /translation="HLHFDHCGGGIFRDSHGKFQLTFPNALYHVSAAQWEWATHPNSR
                     EDDSFLPENILPMKELGALHLIESEGELIPGLSLRIFNGHTRGQLIPLISYRGKTLVF
                     CADLFPFTAQIPLPWIMSYDVEPLKTLQEKEQFLNEALRGNYILLYEHDYFADCSRLQ
                     LTAKGIRATEGFSFQQLLKELNE"
BASE COUNT          510 a          401 c          432 g          546 t
ORIGIN      
        1 cagtccgcag atgaatttga acttgaagtt cctctttcgg ttgctctgaa atagcacaaa
       61 agcatcaacc cgctaacaaa agtagcaggt aattgttcaa ccctcaccgg ggttattatt
      121 tcagcttcta ctccctgctt tttatattgc agcatcggga aaaaaatcat gcctatcccc
      181 tttgaaaata aaaactctac cctaaaccct taggtcatgg cctaaagtag cattatctac
      241 tcctccagaa attttttttt ggatcaaaaa cagtagtttt tattgtgctc aatgcagcgg
      301 atgacttaaa ttttcgtcat ttttgtataa ctaaaaaaac cagaatatgg atgtagaaac
      361 aagaaccaca gccggacagg ttatgggtgt tatcgggata gtgttaggag ttgtttcgct
      421 ggtcttggcc tttataccat gtattggcgt tatggctttc attccgggag tgctggccct
      481 tactttcagc ataatctcta ttgtgcaggc ttcgaatggt tatgggtcta agggcctggg
      541 tataggtgcc ttgattgttt cgctggtatc tattgtgctt gctgccgcat ggcttattgt
      601 ctttagtggc acatttgcct ttgccgacag ggcaattagg aatagcgaca agatagaaat
      661 ttttggcaag gaatttggca aggcctttga ggaagagttt ggcaagggag atgtgaactt
      721 aaaaattgtt tccgacagcc tcgaaaactc acttcgccag ctcgaaaacg agctggacag
      781 tctggatggc tacataccgg aagagaaggc tcgcaaggca ggacaagctg ctgccagagc
      841 cttaaaaaaa tctacacggg tggtaattga aaccgactcg ggtaagattc aatttgaaac
      901 cagcaataaa agccattaaa gtagttgaaa cctccgtctt gcaaaaaggg tattaacgtt
      961 tacgcttctg ttaatgcact ttttattgtc gttatcctgg cagtttttat gtactccttt
     1021 gcgttcaaga ccgaaaaata tccaattcct gcactgctca cccaaagtac agggttaata
     1081 cctccgagcc ggggactttc aaaaagtttt tctgaaattg taaggggaaa tatatccgca
     1141 gcaaagctta tcaaccctca ttctttgcag atattcgcat tttttttgat tcagctggtc
     1201 atgcgggttg ccgggtttgc agcagctaaa attattggca gcccatttcc cgtaaaaggt
     1261 atcgcagtag atgtttgcat atcgctagcg ctttttgtct tctgctttgc cccgcttatt
     1321 tcatatactt tcaggctatt cgttcagctc ttttagaagc tgctgaaacg aaaaaccctc
     1381 cgttgccctt attcctttag cagtaagctg aaggcggctg caatcggcaa agtaatcgtg
     1441 ttcatacagt aaaatgtagt tcccccgaag cgcctcgttg agaaactgct ccttttcctg
     1501 aagcgtttta agcggttcaa catcgtagct cataatccag ggtaaaggaa tttgggcagt
     1561 gaaagggaaa agatcggcac agaataccag cgttttccca cgatagctga ttagcggaat
     1621 aagttgtccg cgggtgtggc cgttaaaaat tctaagagaa agtccgggta tcaattctcc
     1681 ttcggattct atcaggtgta gtgcacccaa ctccttcata ggaagaatat tttcaggaag
     1741 aaaggaatcg tcctcccggg agtttgggtg ggtggcccac tcccattgtg cggccgaaac
     1801 atggtaaagg gcattaggga atgtcagctg aaatttccca tgggaatcac ggaaaatccc
     1861 tccaccgcag tggtcaaaat gcaggtggg
//
LOCUS       DAOOQX010000424         1886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_77293_length_1886_cov_2.876570, whole genome shotgun sequence.
ACCESSION   DAOOQX010000424 DAOOQX010000000
VERSION     DAOOQX010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77293_length_1886_cov_2.876570"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PLX53_07040"
     CDS             complement(<1..729)
                     /locus_tag="PLX53_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOY72874.1"
                     /translation="MRIALAQLNYTINHFDLNRGKIVDAIERAKSEGADLVVFSELAV
                     CGYPPRDLLEQEEFIQKCYSSINAIASCCSGITAIVGAPSLNPNPKGKMLYNSAFVLQ
                     NGVVHSIHGKTLLPDYDIFDEYRYFEPADDGYDLVEVAGKRLAVTVCEDLWDDQPTGE
                     ANFRRHKLYRSSPMEHMAMLKPDIIINIAASPFSYNIEQQRLQVLQGNIVRYGLPVIY
                     VNQVGGNAELLFDGGSMVLDANGAV"
     gene            complement(768..1430)
                     /locus_tag="PLX53_07045"
     CDS             complement(768..1430)
                     /locus_tag="PLX53_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOY72875.1"
                     /translation="MTKRLVHVMAFLMLTSVGVNAQKFSFGVFVDPQLTWFNSDTKRY
                     DPNGPVAGFNIGFSGEKYFAQRYALSSGLSLNTLGGNIRFMQDTFTLKTNDGEYTVTP
                     QTNVKFRAQYLTLPVSLKFRSTQIGYTSFYANVGFKGSIRLKGFAWYSPQNVDREVAN
                     DHFAWGFVSYFIGLGMEYSLGGESALQCGLSFSDGLTGMLDTSNGKITSQSLTLRLGI
                     IF"
BASE COUNT          561 a          450 c          387 g          488 t
ORIGIN      
        1 tactgcccca ttggcatcga ggaccatgga accgccgtca aacagcagct cggcgttacc
       61 accaacctgg ttgacatata taaccggcaa cccgtaacga acgatatttc cctgcaaaac
      121 ctgcaaccgt tgctgctcga tattgtagct gaacggagaa gcagcaatgt taataattat
      181 gtcgggcttt aacatcgcca tgtgttccat agggctgctg cggtatagct tatgccgcct
      241 gaaatttgct tcaccggtag gctgatcatc ccataaatcc tcacacacgg taacagccag
      301 gcgttttccg gcaacctcaa ccagatcgta gccatcgtct gcaggctcaa agtacctgta
      361 ctcgtcaaat atgtcgtaat cgggtaaaag cgttttgcca tgtatgctgt gaactacgcc
      421 attctgcaac acaaaagcag agttgtagag catcttcccc ttaggatttg ggttaaggct
      481 aggagcgcca acaatggcag ttataccgct acagcaggat gcaatggcgt taatagagct
      541 gtagcacttc tgaataaact cctcctgttc cagcaagtcg cgcggagggt agccacatac
      601 agctagttcg gaaaaaacca ccaggtctgc tccttctgat ttagcccttt cgatagcgtc
      661 aacaattttc cccctgttca ggtcaaaatg gttgatggtg tagttgagct gggcaagcgc
      721 aattctcatt ttgcaaaatt catttggccc gacaagccta aaaccgttta aaaaattatt
      781 ccaaggcgca aagtaagcga ctgggaagta attttaccat tagatgtatc aagcattccg
      841 gttagaccgt cggaaaacga taggccacac tgaagagcgc tttcgccacc tagagagtac
      901 tccataccca gcccaataaa gtaggaaaca aaaccccaag caaaatggtc gtttgccacc
      961 tccctgtcaa cattctgtgg gctgtaccat gcaaacccct ttagcctgat gctgccctta
     1021 aacccaacat ttgcataaaa gctggtgtag ccaatttggg tagatcgaaa ctttaagctc
     1081 accggcaggg taaggtactg agccctaaac ttaacatttg tttgaggagt aacggtgtat
     1141 tcgccatcat tggttttcag ggtgaaggta tcctgcataa acctgatgtt tccacccaga
     1201 gtattcaacg atagtccaga tgatagggca taccgctggg caaagtactt ctcgcccgaa
     1261 aagccaatgt tgaaccctgc tactggccca ttgggatcgt agcgcttggt atcggagttg
     1321 aaccaagtaa gctgtgggtc aacaaaaaca ccaaagctga acttttgggc attaaccccc
     1381 acgcttgtaa gcattaaaaa tgccatcaca tgcactaacc tcttcgtcat catcctaaaa
     1441 aattaattat tagaatagta gtctagcata aatcattttg aagtaactct tacgggtaca
     1501 aaagaatggg atacaaacgt acatgataat tttcaattaa ccctcacacg caggaaaaac
     1561 ttttactgac actccaacaa agcagaagag acatatttgc acagattttt tacccgtgtt
     1621 tgtcagtagt atcagagtaa tccatgttcc acttttctgt tctagtgata tagaagaaaa
     1681 ggaaccattt tcagctgcat tgcgcattaa catgcttact tctgcattcc tacaacaata
     1741 tcattcaaac catttcaagt ttaaccaaag gttaagggta gccaaggttg caaatttacg
     1801 atgtgtgtcg aaatgaacat ttgctaaatt tgccgtaaaa atggataatg atagtcttcc
     1861 ccaatgctaa aatcaacatc ggactc
//
LOCUS       DAOOQX010000425         1884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_77418_length_1884_cov_3.390924, whole genome shotgun sequence.
ACCESSION   DAOOQX010000425 DAOOQX010000000
VERSION     DAOOQX010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77418_length_1884_cov_3.390924"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..652)
                     /locus_tag="PLX53_07050"
     CDS             complement(128..652)
                     /locus_tag="PLX53_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOY72876.1"
                     /translation="MGRKSLQVQGYSSEEIRVLFTKDERYTIGIRLYAVYQVSLGQAS
                     RKLESLYNTSFKQITNWVHRFEEFGLEGLKDKPKSGRKPRLAEEQLNELSSVLQNNRP
                     DEFGYNTATWNGPILKDYIEKHYHVAYQKAQVYNLLKKLGFTYQKSTSQYPEADEGKR
                     GEFKTTLKKTKRGT"
     gene            964..1176
                     /locus_tag="PLX53_07055"
     CDS             964..1176
                     /locus_tag="PLX53_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72877.1"
                     /translation="MRMLSVAYLVATSCCTVLYIPNSYNSPLLRGKEDARANFPAGTP
                     GFMSKMGYKRVRFYTCIGVSLPFTHL"
     gene            complement(1317..1877)
                     /gene="purN"
                     /locus_tag="PLX53_07060"
     CDS             complement(1317..1877)
                     /gene="purN"
                     /locus_tag="PLX53_07060"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOY72878.1"
                     /translation="MAILASGSGTNAENIIRYFSNHPLIRVTLVATDNPNAYVIERAK
                     TSAVPVVVFSMEELRNGVLLNILKLNNIDLIVLAGFIKLVPEQVVNAYPGRIVNIHPA
                     LLPAYGGKGMYGNNVHEAVLANGERESGITIHYVNNRYDEGDIIFQAKCEVNPSDTPD
                     TLAQKVHVLEYQHYPRVIESLLTSLI"
BASE COUNT          458 a          437 c          388 g          601 t
ORIGIN      
        1 tgtttggcgc tctcttctgt tttgctttga cactaccctg ggttgctttc cacgaaccgc
       61 ccagctgtat gatatcgttg ccgtgttgct taagctatac tcatcctcaa atagaataac
      121 ggtgctatca ggttcctctt ttagtttttt ttaatgtcgt tttaaactcc cctcttttcc
      181 cctcatccgc ttcgggatac tgggatgtgc tcttttgata ggtaaaacca agcttcttca
      241 gcaagttgta aacctgcgct ttttggtatg ctacatgata gtgtttttca atgtaatctt
      301 ttagtatagg cccattccat gtagctgtat tgtagccaaa ctcgtcgggt ctattatttt
      361 gcaacacact actcagctca ttcaactgct cttcagctaa ccttggctta cggccgcttt
      421 tgggtttgtc cttcagcccc tctaatccaa attcttcaaa tcgatgtacc cagttggtga
      481 tttgcttaaa actggtattg tataagcttt ctaatttgcg actggcttgc ccaagtgaaa
      541 cctgataaac cgcgtacagc ctgatgccga tggtatatct ttcatcctta gtaaacagaa
      601 ctctaatttc ttctgaacta tagccctgaa cttgtaatga ttttcttccc ataatgtagt
      661 aatttagttt tttcaaaatt actacattta atgtgtagtt acaaattata ctttatataa
      721 atgaatgtga tccacaggtt ggcgtgtggg cgttttttaa ctttcaaatg gctgaccttt
      781 ttttgtgcta tttcgtttcc ggtggattag gagaggcatt caatttaccg gtgggcggta
      841 cctacctatg gttggcgttt atgtggggag aagaatgatt tgtgcaaggc aacctgacgt
      901 tgagtttgag ccatacattt aaatatttta aaaaccgatg agaggaatgc tgcccaaaac
      961 tttatgagga tgttgtccgt tgcctacctg gtagccactt cctgttgtac cgttttgtat
     1021 attcccaaca gctataactc ccctttactc aggggcaaag aagatgcccg ggccaacttc
     1081 ccggctggaa ctccaggctt tatgtccaaa atgggctaca agcgggttcg attctacacc
     1141 tgcataggag tatccctgcc attcacgcac ttataagggc gaccgggatt acaacccgtt
     1201 tacattcccg gtaggtcttc actttaattt tggcaggcat aaccagaaat agctgctgga
     1261 taagccatac cccggctgct tgttgccggt tacatgcccc aaataggcaa ttcagcttaa
     1321 ataaggctgg ttagtagcga ctcgattacc cttgggtagt gctggtattc cagcacatgc
     1381 actttttggg caagggtatc gggagtatcg gatgggttta cctcgcattt agcctgaaaa
     1441 ataatatcac cctcgtcata ccggttattc acgtaatgta tggtaatacc actttccctc
     1501 tcaccgtttg ccagaaccgc ctcatggaca ttgttcccat acataccttt acccccgtat
     1561 gccggtagca gggcgggatg aatattcaca attcgtcctg gatatgcatt gaccacctgt
     1621 tccggaacaa gcttaataaa accagccagt acaattaagt ctatattatt caatttcaga
     1681 atattaagca gcactccgtt acggagctcc tccatggaaa acaccacaac cggaaccgcc
     1741 gacgtttttg cacgctctat tacgtaagca ttagggttgt cggttgccac taacgttacc
     1801 ctaataagtg gatgattgct gaagtagcgg atgatgtttt cggcattggt gcccgatccc
     1861 gaagccagta tagctatatt gttc
//
LOCUS       DAOOQX010000426         1883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_77485_length_1883_cov_3.425055, whole genome shotgun sequence.
ACCESSION   DAOOQX010000426 DAOOQX010000000
VERSION     DAOOQX010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77485_length_1883_cov_3.425055"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..870)
                     /locus_tag="PLX53_07065"
     CDS             complement(37..870)
                     /locus_tag="PLX53_07065"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOY72879.1"
                     /translation="MKHKPIIHVVFTLLLLVVSEATLSQSVNPCGIVNRSFASGEKLV
                     FEISYTWLFVWTDVGMAELTVSAEKRFGRDLLHIKASGKTHTFYNWFFEVHDLYETWV
                     DPVTVEPVYFNRDIYEGGFTKENEYFYDWNKNQVAIRVRRKKGPNRYDTLSVSRCTYD
                     VISAVYAARNLDFSGIKPGKTFPVEVLMDEEVYHIGYRFLGREEKRVSGLGRFRCLKF
                     QIDVVAGDIFSGDQKLYLWVTDDANKLPVAVETPIQVGSIKARLNTYEGLRHPLSSKI
                     P"
     gene            complement(1038..>1883)
                     /locus_tag="PLX53_07070"
     CDS             complement(1038..>1883)
                     /locus_tag="PLX53_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOY72880.1"
                     /translation="LVMTATPIPRTLAMTVYGDLDVSVIDELPPGRKPVKTLYYTDLK
                     RNVVFGLMRQQLKEGRQVYVVYPLIKESEKMENLKDLQDGYESIVRAFPPPEYVTVVV
                     HGQMKPEEKESSMDIFVKGKANIMVATTVIEVGVNVPNATVMVIESAERFGLSQLHQL
                     RGRVGRGADESYCILVSSLKISSESRKRLQIMVNTSNGFEIAEMDLKLRGPGDIEGTL
                     QSGIPFDLKIANLSTDGNLLQHARAVARDVLNDDPLLEHPRNALLSQRLDELANSRSN
                     FGRIS"
BASE COUNT          515 a          470 c          379 g          519 t
ORIGIN      
        1 ctaatttatg gaaaatagtt gtcataccca tacaacttag ggaatttttg atgataacgg
       61 atgccttaat ccctcgtatg tgttaagcct ggcctttatg cttcccacct ggattggagt
      121 ttccaccgca acaggcagct tgtttgcatc atcggtaacc cacaggtaga gcttttggtc
      181 gcccgaaaag atgtcgccgg ctaccacgtc aatttgaaat ttaaggcacc tgaaacgtcc
      241 aagccctgaa acccgttttt cttcgcgtcc aagaaacctg tacccgatat ggtaaacctc
      301 ctcatccatc aaaacttcaa caggaaatgt tttacccggc ttaatacctg agaagtccaa
      361 atttctagct gcataaacag ccgaaatcac atcgtaagtg cacctgctaa cactcaaggt
      421 atcgtatcgg ttaggccctt ttttacgacg tacccttatg gctacctggt ttttgttcca
      481 atcatagaag tactcgttct ccttggtgaa tccgccctca tatatgtcgc ggttaaagta
      541 aaccggctca acagtaaccg gatcgaccca tgtttcgtaa aggtcgtgga cctcgaaaaa
      601 ccagttgtag aatgtatgcg ttttccccga agccttaatg tgcagtaaat ctctgccaaa
      661 acgtttctca gcgctgactg taagctcggc cattcctaca tctgtccata caaaaagcca
      721 tgtatagctt atctcaaaaa ccaacttctc acccgatgca aaagacctgt tgacaattcc
      781 acagggattg accgattgcg atagggtagc ctcgctgaca accaaaagca aaagagtaaa
      841 aacaacatgt atgatgggtt tgtgtttcat tacgattctt attatcagca atcaactatt
      901 ttattgtaag ctagattatg aacatctagt tacttcaaca ctaaaaagta tattggaaat
      961 gaaggttcat tacaccccaa aaataatacg ataaaaatta ctactaagct aaaattttcc
     1021 cgcgtaacga ttagctgcta gctaattctt ccgaagttac tccgtgagtt ggcaagttca
     1081 tcaagccgtt gtgaaagaag tgcattccgg ggatgctcta atagcgggtc atcattaagt
     1141 acatccctgg caacagccct tgcatgctga agtagatttc catcggtgct aagattggct
     1201 attttcaggt caaagggaat tccgctttga agagtccctt caatgtcgcc gggcccgcga
     1261 agtttgaggt ccatttcggc aatttcaaaa ccattgctgg tattaaccat aatctgtagc
     1321 ctcttacgcg attcagaaga aatttttaag gatgagacaa gtatgcagta gctttcgtcg
     1381 gctccccgcc ctaccctacc gcgcagctgg tgcagctgcg acaggccaaa cctctcggca
     1441 ctctcaatta ccataacggt tgcattggga acattaaccc ctacctcgat aacagttgtt
     1501 gccaccatga tgttggcctt gcctttaaca aaaatgtcca tggaggattc cttttcctcg
     1561 ggtttcatct ggccatgaac cacaactgta acgtattcgg ggggcggaaa tgcccgtacg
     1621 atgctttcat agccatcctg aaggtctttc aggttttcca ttttttccga ttcctttatc
     1681 agcgggtaaa ccacgtaaac ctgccggccc tccttcagct gctggcgcat caggccaaaa
     1741 actacatttc gcttcaaatc ggtgtagtac agcgttttaa caggctttcg gccaggtggc
     1801 agctcatcaa tcacgctaac atccaaatca ccgtaaactg tcatggccaa ggttctcgga
     1861 attggcgtgg cagtcataac cag
//
LOCUS       DAOOQX010000427         1876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_77980_length_1876_cov_2.336628, whole genome shotgun sequence.
ACCESSION   DAOOQX010000427 DAOOQX010000000
VERSION     DAOOQX010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77980_length_1876_cov_2.336628"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PLX53_07075"
     CDS             complement(<1..960)
                     /locus_tag="PLX53_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72881.1"
                     /translation="MSKVRENLLVVVLVLFIARMGLPQYLKYILFPAVGLYGVWALWQ
                     FVEDRRWQGFRLRNTLIFTPLLVSMLIYFIMLVFTPNPQINLLRDAFNVLVFFSFVIA
                     LYLISYTPSGYQKVLNQVALYTFIISSLFAFLGVLKLVLQLYGITFEFLEVEMLGYPL
                     GTSLSVDNNSFAILCLLGLVLAIPYTTRKLKIRYSLLLQLGLTLIVVSAFFSTSRRGL
                     IIALLCLLICGLTWLVSIPFRSERLKNLRVNTSFFLLLSIMVIGNFYWFVNHMSPIER
                     YRFLYSHHFEKFEAIHFINRMAVQEQLISNGNTEYSDVEWKLWG"
     gene            complement(1075..>1876)
                     /locus_tag="PLX53_07080"
     CDS             complement(1075..>1876)
                     /locus_tag="PLX53_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72882.1"
                     /translation="VYCFVSPDFNGNDVRLELRGPVKGQTLSRFNFNERGRWVKLEAK
                     AIADKEGIVSGIFLFMQNNVKDFSALKGYVTFAYPQLYVKPKVGLTEVISDYKINLAG
                     FAGLGLQLGTSVDSTGSDGEFKFSMADDRFAGPRVDRWRYAIYLYRHEYPPLQKIFGG
                     GFGYTRKFAQQFLMDDRQLDYDYPHNPFLSILLYSGMLGLLISVWFIYQVFRLYWSHR
                     KVLWPFGLAFLASFFYAFFSSNNFFEPAIFGVLAFIPFYYKYLSVVKD"
BASE COUNT          624 a          427 c          360 g          465 t
ORIGIN      
        1 accccaaagc ttccactcaa catcggagta ctccgtgtta ccattcgaga tgagctgctc
       61 ctgaacggcc atcctattaa taaaatgtat tgcctcgaat ttttcaaaat ggtgactgta
      121 tagaaacctg tatctctcaa taggggacat atgattgaca aaccagtaaa agttgccaat
      181 taccattatg gatagaagca aaaaaaagga tgtgttaacc ctaaggtttt tcaacctctc
      241 actcctaaat gggatggaca ccaaccacgt caagccgcaa atcagcaagc agagcaatgc
      301 gataatcagt cctcttcgag aggtagaaaa aaatgcactt acaacaatca atgttaaccc
      361 cagctggagt aataggctat atcgaatttt gagttttctg gtagtatatg gtatagcgag
      421 aaccagtccc agtaagcaaa gtattgcaaa ggagttgtta tcaacactta gcgatgttcc
      481 taaaggataa cccagcattt caacctctag aaactcaaat gttattccgt agagctgcaa
      541 aaccagcttc aaaactccca ggaaggcgaa aagggaagat atgataaagg tatagagtgc
      601 tacttgattc aaaacctttt gataccccga tggggtgtag ctaattaggt atagcgctat
      661 aacaaaagaa aaaaatacca acacgttgaa agcatcccgt aaaaggttga tttgcggatt
      721 aggagtaaac accagcataa taaagtatat cagcatggaa actaaaagtg gggtaaaaat
      781 cagggtgttt cttagcctaa aaccctgcca tcttctgtcc tcaacgaatt gccatagcgc
      841 ccaaacgcca tacagaccaa ctgctgggaa aaggatgtac ttcagatatt gcggtaatcc
      901 catcctggct ataaaaagca ccagcaccac gaccaataga ttttccctta ctttcgacat
      961 gaattaaatt gttactacta ttccgccatc tacattcatc taattttcta tgttattcgc
     1021 cttatatata gactattaga agcataaagc gcacctaaca cctatttaaa cttgttaatc
     1081 cttcacaaca gataagtatt tgtaataaaa tgggataaat gccaaaaccc caaatattgc
     1141 gggttcaaaa aaattattac tgctgaaaaa agcatagaag aacgaggcca ggaaggctag
     1201 cccaaacggc caaagcacct ttcgatggct ccagtacagg cgaaatacct gataaataaa
     1261 ccagacgcta atcagcaaac ccagcattcc ggagtagagg agtattgata aaaatggatt
     1321 atgagggtaa tcgtagtcga gctgccggtc atccattaaa aattgctgtg caaattttct
     1381 ggtgtagcca aagccgcctc caaatatttt ctgtaatggg gggtactcat gcctgtatag
     1441 gtaaatggca taccgccacc ggtcaactct tggaccggca aacctgtcgt ccgccatcga
     1501 aaatttaaat tcgccatcgg agcctgtaga atcaacagaa gtccccagct gcaaacccag
     1561 cccggcaaaa cccgccaaat taattttata gtctgaaatc acctcggtaa gtccaacctt
     1621 aggttttacg taaagttgtg ggtaggcaaa ggtaacgtac ccctttaggg cactgaaatc
     1681 cttcacattg ttctgcataa aaaggaaaat acccgaaacg attccctctt tatctgcaat
     1741 agccttagcc tctaacttta cccatctacc acgctcattg aagttaaaac ggctaagtgt
     1801 ttgtccctta accgggccac gcagctccag ccgcacatcg ttaccgttga aatcgggcga
     1861 tacgaagcag taaact
//
LOCUS       DAOOQX010000428         1863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_78791_length_1863_cov_3.159845, whole genome shotgun sequence.
ACCESSION   DAOOQX010000428 DAOOQX010000000
VERSION     DAOOQX010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78791_length_1863_cov_3.159845"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PLX53_07085"
     CDS             <1..541
                     /locus_tag="PLX53_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOY72883.1"
                     /translation="AASAVTSILQYFSSEVSLKSFVIWTMGSLSSISGNQLWIFALLV
                     FLGLVLAMLVARPLNVLGVGENYAQLLGYNVKAIRMVVFISTSLLAGSTTAFVGPVVF
                     VGIAVPHVVRMAVKTSNHHVLLPTTLLAGAAFMLLIDTIAQLPGAGIVLPVNTVAALM
                     GIPVVIYVIFKGSHFHAAS"
     gene            528..1553
                     /locus_tag="PLX53_07090"
     CDS             528..1553
                     /locus_tag="PLX53_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY72884.1"
                     /translation="MQPAEVIGIKNLVVGYGEGAMSDFTYPPINANAYKGEMVALVGR
                     NGAGKSTLLRTISRLQPMMNGEITVNGATLESIGRSEFSQLVSFIPAEPVRLPNTTVS
                     DFITLARYPYHGWFDGLSHTDTTMVGKALKAVNLFDYRNRFLDRLSDGERQRVMVAFA
                     IAQDTPVILMDEPTAFLDLPSKFEIVRLLREQTLLGKTVIISTHDLQTAFGFVDTIWL
                     MLREGIRIGSPEDLVLTGAIDSLMDGTLVKFDMSSGQFKYTNSKTLSAYVSGSKRSLV
                     KWTAHALERLGYHVSSIVLDTTSLQVEVMETEGKIQWCIENHKHKPTFDSLRNLCLFI
                     KLEMNAG"
BASE COUNT          483 a          422 c          458 g          500 t
ORIGIN      
        1 tgctgcctca gcagtaacca gcattttaca gtattttagc agcgaggtat ccctcaaatc
       61 gtttgttatt tggactatgg gcagcctaag tagcattagc ggaaaccagc tatggatttt
      121 cgccctgctg gttttccttg ggcttgtgct ggccatgctg gttgcccgac cattgaacgt
      181 gctgggcgtt ggagaaaatt acgcccagct gctgggctat aacgttaaag ctatcagaat
      241 ggtagttttt atctcaacca gcctgctggc cggttcaacc actgcatttg tcggtccggt
      301 tgtttttgtt ggtattgctg ttcctcatgt ggtgcgaatg gccgttaaaa cttccaacca
      361 tcatgtgctt ttacccacca cgctattagc aggtgctgca tttatgctcc tgattgatac
      421 tattgcccag ctgcccggcg ccggaattgt gcttccggtg aataccgtgg cagcgttaat
      481 gggcattcct gttgtaattt acgtaatctt taaaggtagc cacttccatg cagccagctg
      541 aggtaatagg aattaaaaac ctggtagttg ggtatggtga gggggctatg tccgatttta
      601 cctatccacc tatcaatgcc aacgcttata agggagaaat ggtggcgctg gtgggacgga
      661 acggagcggg aaaaagcacc ctgctgcgca ccatttcacg gcttcaaccc atgatgaatg
      721 gcgaaattac cgtaaatggc gctactttgg aatcaattgg acgctctgaa ttttcacagc
      781 ttgttagctt tattccggca gaacctgttc ggttgcctaa taccacggtc tctgatttta
      841 ttacacttgc ccgttacccc taccatggct ggtttgatgg gctcagccac accgacacga
      901 ctatggtggg caaagccctt aaggccgtaa acctgtttga ctaccggaat cgcttcctcg
      961 ataggctgag tgatggcgaa aggcagcggg tgatggttgc ctttgccatt gcccaggata
     1021 cacctgtaat cctgatggac gagccaacgg cattcctcga tttgccgagc aagtttgaaa
     1081 ttgtaaggtt actccgcgag caaaccctgc tggggaaaac ggtcatcatc tcaacccacg
     1141 atcttcaaac tgcctttggc tttgttgaca ccatctggct tatgcttcgc gagggcatta
     1201 gaattggttc gcctgaggac ttagtgctaa ccggtgctat agacagcctg atggatggaa
     1261 ccctggttaa gttcgacatg agctccggac agtttaagta caccaactcc aaaaccctta
     1321 gcgcttacgt ctcgggaagt aaacgaagct tggtaaaatg gactgcccat gcgttggaac
     1381 gacttggcta ccatgtttca agtattgttt tagatacaac ttccctgcag gttgaggtga
     1441 tggaaaccga agggaaaatt cagtggtgta tagaaaacca taaacataag cccaccttcg
     1501 attccctaag aaacctttgc ctgtttataa agctggaaat gaatgctggg tagggcgtgg
     1561 agcagggaaa tgctacaagt gaaccgggaa ccggggttgg tgaaccgtaa accgaataaa
     1621 caaaaagtta actccccgtt acagcacgaa ccgacccgcc accaggtggg gagctcggcg
     1681 aagctaaccg tgcggcagca atgggacata taatttattc caatacgaag aagttcttcg
     1741 gttaaatata ttgcaaggct aaaaagtaga tagatagata aaaatatttt tgcaaaaaaa
     1801 agtttgatac aacattgaaa tttttatatt aactttcaac cgattttgaa aaatcgttaa
     1861 tgg
//
LOCUS       DAOOQX010000429         1862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_78876_length_1862_cov_2.523520, whole genome shotgun sequence.
ACCESSION   DAOOQX010000429 DAOOQX010000000
VERSION     DAOOQX010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78876_length_1862_cov_2.523520"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLX53_07095"
     CDS             <1..555
                     /locus_tag="PLX53_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOY72885.1"
                     /translation="PGLSLELLKMGPVVPPGHQEIAFSDFRFATPSGKIELLSEQVAS
                     LWNANPLPEFLPAMEISDANSTYPLLLLTPNTKNRIHSQFGNLKIIKFINGNPVVQMN
                     PSDAMHRGIKNGSLVKVYNARGSIQVSAQPTYEVRKGCVVIYNGWWKSQGAALNTLSA
                     GRETDMGHGAAFHNNRVEVNLVKK"
     gene            559..>1862
                     /locus_tag="PLX53_07100"
     CDS             559..>1862
                     /locus_tag="PLX53_07100"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HOY72886.1"
                     /translation="MQYNGFIFDYARCVGCHACVVACYNENGVQPPVLWRQVVGVNPE
                     KVPLYGFVSLSMACNHCTDAPCLKACPASAYYRDQETGAILHSNKRCIGCKYCTWACP
                     FDAPKYNPLRGVVEKCTMCNERLKSGEIPACTEACPTGALSFGSIETAASIQAPGISA
                     KPFSPRIVTKNEDIVNHIPHIDEQVTGYSPLQHTGMKHFAPSKINPIHELPLAIFTLI
                     CSLMVGWVGSMPWVGIASRGITLFILLGIVGALLSTFHLGKPFRALRSVANVKSSWLS
                     REILLFTLFFSLSALNLIFFNLIVLSVIAVILGLLLLVAVEMVYSVTDKKYKIHVHSA
                     NVVITALTIFSIFNSFERLLVLVLVLKAALYILRHANYSVSSLEFIFSFIRVFLGILI
                     PLGSIFFSNQHISMLMILFILIGELIDRIEFYGEQQVDTPRLL"
BASE COUNT          481 a          392 c          415 g          574 t
ORIGIN      
        1 cccggattat cattagagtt actgaaaatg ggtccagttg ttcctcctgg acatcaggaa
       61 attgcctttt ccgattttcg ctttgcgacc ccgtcgggta aaattgagtt gctttcggaa
      121 caggttgctt ctctctggaa tgcaaatcca ctaccggaat ttttgcctgc catggaaata
      181 agcgatgcca atagtactta cccattactg cttttgactc caaatacaaa aaacaggatt
      241 cattcccaat ttggcaacct gaaaattata aaattcatca acggcaatcc tgtagtgcaa
      301 atgaacccat cagatgctat gcatagaggc attaaaaatg gtagcctggt aaaagtttac
      361 aatgcccgcg gtagtattca agttagtgca cagccgacct acgaggtaag aaagggctgc
      421 gtggtgattt ataatggttg gtggaaatct cagggagctg cactcaacac gctttctgcc
      481 ggtcgcgaaa ccgatatggg acatggggct gcttttcata ataacagggt ggaggttaac
      541 ctggtaaaga agtaagcaat gcagtacaac ggttttatat tcgattacgc cagatgtgta
      601 gggtgccatg catgtgtggt agcctgttat aacgaaaacg gagttcaacc acctgttttg
      661 tggaggcagg tggttggtgt gaaccccgaa aaagttcccc tttatggatt tgttagctta
      721 tcaatggcat gtaaccattg tactgatgcc ccatgcctga aggcctgccc cgcttctgct
      781 tactatcggg atcaggaaac tggggctata cttcatagta acaaaagatg cattggttgt
      841 aaatactgca catgggcttg cccctttgat gcgccaaagt ataatccctt aagaggggtt
      901 gttgaaaagt gcaccatgtg taacgaaagg ttgaaaagtg gagaaatacc ggcctgtact
      961 gaagcatgcc ctacaggtgc tctctcattt ggcagcattg aaactgcagc tagcattcag
     1021 gcacccggca tttccgctaa accattttct cctagaattg ttactaaaaa cgaggatatt
     1081 gtaaaccata tcccccatat cgacgaacaa gttacgggct atagcccctt gcaacataca
     1141 gggatgaagc attttgcccc ttctaaaatt aaccccattc atgagttgcc tttagccatc
     1201 tttacgctaa tttgctcgct tatggttggg tgggtgggtt cgatgccatg ggtaggcatc
     1261 gcttcaaggg ggataaccct gtttattctg ttaggaattg taggggctct actgagcacc
     1321 ttccacctag gcaagccctt tagggcatta cgttccgttg ctaatgttaa gagcagctgg
     1381 ttgagccgtg aaatcctgct ttttaccctc tttttttcgc tgtcagcatt aaacctgatt
     1441 ttttttaacc tcatcgttct atcagtgatt gctgttattt tggggttgct tcttttagtt
     1501 gctgttgaga tggtttactc cgttactgat aagaagtata aaattcatgt tcatagcgca
     1561 aatgttgtga ttacagctct gacaatcttt tcaattttca acagctttga gcgcctactt
     1621 gtcctagtgc ttgttctcaa ggcagcctta tacatacttc gacatgcaaa ctattctgta
     1681 agttcattag aatttatttt tagcttcatt cgggtatttc tgggaattct tattcccctc
     1741 ggctcaatat ttttctcaaa ccaacacatt tctatgttga tgattttgtt cattttgata
     1801 ggagagctga ttgataggat tgaattttat ggggagcagc aggtagatac tccacggtta
     1861 ct
//
LOCUS       DAOOQX010000430         1857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_79199_length_1857_cov_2.601554, whole genome shotgun sequence.
ACCESSION   DAOOQX010000430 DAOOQX010000000
VERSION     DAOOQX010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79199_length_1857_cov_2.601554"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            416..>1857
                     /locus_tag="PLX53_07105"
     CDS             416..>1857
                     /locus_tag="PLX53_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOY72887.1"
                     /translation="MSKKGEKPSGRTPQKRGVTKRDLREGLMDVFIKNPNQSFNYKQL
                     STRLGLNNDNTRRMITEVLYELVGEERLVELSRGKFRLQEQTSLATGILVMSKDGSGE
                     VTTDDNREIFIPDFRLNHALHGDRVEISIYRHRRMGLLEGEVIRVIERAKRNFVGTIS
                     LLRNHAFVVPDNKLMPYDIFIPHVELRDIKNGQKVIVQITDWPPREKNPNGKIVEILG
                     NPGVHEVEMHAILAEFDLPYRFPEELEKLAEKIDDRISNADYASRRDFRDVPTFTIDP
                     FDAKDFDDALSVQILPSGNIEVGVHIADVTHFVKPNTPIDNEAIERGTSVYLVDRCVP
                     MLPEKLSNYICSLRPNEEKLCFSAVFELNDNAEVLSEWFGRTVIISKRRFTYEEAQAI
                     IETGEGDLHEQILLLHKLAQKLRRERFKNGAIAFERMEVKFELDAKGKPLGVYFKENK
                     ESNQLIEEFMLLANRKVAELVGKQKPGNER"
BASE COUNT          515 a          404 c          465 g          473 t
ORIGIN      
        1 gttttcgtct aaagcccttt cggaatcttt ctttcatgag ccatcctcta tgcccacctg
       61 ttcctttgaa gtttgggggt ttacttgctc ggctaagtct ttgacttagt cgtaaccaag
      121 tagcggtttc tgacggcgta agcttcagct tatagctagg cttaagggtt gtattacgtg
      181 tgtggctttg acctaaaatg gggaagtgta atttttttaa cggcggtcgg caggccatga
      241 ttttaaaacc gacaaagtta taagcttcat cgagtgggtt gggagaaaat gaaaatcggt
      301 tttttacacg aatgatagag tctttgctgc gctctttaaa attttaaaat caggatagcg
      361 caaaaacagc tacatttgaa attctaatat cagcataaaa ctcaaacatt taaaggtgag
      421 caaaaaaggc gaaaagccct cggggagaac tccccagaaa agaggggtta ccaaaaggga
      481 ccttcgcgag ggtctgatgg acgtattcat caaaaaccct aatcagagtt ttaactacaa
      541 gcagctatca acaaggcttg gcttgaacaa cgacaacacc cggaggatga taaccgaggt
      601 gctctacgag cttgttggcg aggaacggct ggttgagctt tcgaggggca agttccgcct
      661 acaggagcag acaagcctgg caacaggaat tctggtaatg agtaaggatg gatcgggaga
      721 ggtaaccacc gacgataacc gtgagatttt tattcctgat tttaggctta accacgccct
      781 gcatggcgat agggtggaga ttagcattta ccgccaccga cgaatggggt tacttgaagg
      841 cgaggttatt agggtgattg agcgtgccaa gcgaaatttt gtcggaacca tatcgttgct
      901 tcgcaaccac gccttcgtgg ttcccgacaa taagctgatg ccctatgata tctttattcc
      961 ccatgttgaa cttagggata ttaaaaatgg ccaaaaggtt attgtgcaaa ttaccgattg
     1021 gcctccccgg gaaaagaacc caaatggaaa aatagttgaa attcttggaa atccgggtgt
     1081 acacgaggtt gaaatgcacg ctattctggc agagtttgac ctgccctaca ggttccccga
     1141 ggaactcgag aaacttgccg agaagattga cgaccgtatt tccaatgccg actatgccag
     1201 caggcgcgat tttagggatg tgccaacatt caccattgac ccgtttgacg ccaaggattt
     1261 tgacgatgcg ctatcggttc aaattttacc aagtggcaac attgaggtgg gtgtccatat
     1321 tgccgatgtt acccacttcg tgaagccgaa tacccccata gacaacgagg ccattgaacg
     1381 gggaacttcc gtttacctgg ttgaccggtg cgttcccatg cttcccgaga agctttcaaa
     1441 ctacatttgc tcccttcgcc caaacgagga gaagctctgt ttttcggcag tttttgagtt
     1501 aaacgacaat gctgaagtcc ttagcgagtg gtttggacgc actgtaataa tctccaagcg
     1561 acgatttacc tacgaggagg ctcaagctat tatcgaaacc ggcgagggcg acctccatga
     1621 acaaatactt cttctccaca agctggcaca gaagcttcgg cgtgaacgct ttaaaaatgg
     1681 cgccattgca tttgagcgga tggaggtaaa attcgaactc gacgccaagg gtaagccact
     1741 tggagtgtac tttaaggaga acaaggaatc gaaccagctt attgaggagt tcatgctgct
     1801 tgccaaccga aaggttgccg agctggttgg caagcaaaaa cctggtaatg aacgatt
//
LOCUS       DAOOQX010000431         1854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_79353_length_1854_cov_3.278488, whole genome shotgun sequence.
ACCESSION   DAOOQX010000431 DAOOQX010000000
VERSION     DAOOQX010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79353_length_1854_cov_3.278488"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLX53_07110"
     CDS             complement(<1..762)
                     /locus_tag="PLX53_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOY72888.1"
                     /translation="MSNEHSRIVEIRNVTAGYGRDVVLKNVSLDIYDDDFIGIIGPNG
                     GGKTTFLKLLIGQVKPFSGEVSYSTSGKQLPIGYLPQAGMLDKNFPITVMEVVLSGLL
                     SQKGLTGRYSKAQRQLANDYLRKTGIEALKHRPIGELSGGQLQRTLLCRALISEPKLL
                     ILDEPNTYVDTNFEGELYEILKELNQRMAIIIVSHDLGTITSYVKTIACINRTLFYHK
                     SNQLSTELLAAYNCPIQIIAHGPVPHTVLLEHEHHA"
     gene            complement(755..1642)
                     /locus_tag="PLX53_07115"
     CDS             complement(755..1642)
                     /locus_tag="PLX53_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOY72889.1"
                     /translation="MRRISGIMVFLVVVLGLMLPACTEKKHTTSKPIVMVSIQPIKYF
                     VEQIADTLVDVRVMVPPGSSPEMFEPTPGQMVDLSNADIYFAIGLLDFEKGLEPKLKD
                     QLKVRYVNLSGKADLIKEGTDEHDSAGGHHHGVDPHIWMSVEEVRKMSMQILQNLQQL
                     YPAHSDFFSNNYIAFGSKLDSLKEQVNRILGKSSNRAFLVYHPALGYYAREFGLRQIS
                     IEQEGKNPSLKGVVDVVDQARRLGVSAVLSQSQFDIRNAQVIADELNLRVVKVDPLAE
                     DWENSMLHIARAISGEHDE"
BASE COUNT          445 a          451 c          420 g          538 t
ORIGIN      
        1 ggcatggtgt tcgtgctcca gcaggacggt atggggcacc ggtccgtgtg caattatctg
       61 aatgggacag ttgtaggctg ccagcagctc agtgcttaat tggttcgact tgtggtagaa
      121 tagggttcgg ttgatgcagg caatggtttt aacataggag gtaatggttc ctaaatcgtg
      181 tgaaacgatt atgatggcca ttcgctggtt cagctccttt agtatttcgt agagttcccc
      241 ctcaaaattg gtatcaacgt atgtgttggg ctcgtcgaga ataagtagtt ttggctcgga
      301 aattagtgct cggcagagca aggttcgttg aagctgtcca cccgatagct cccctattgg
      361 tcggtgtttc agcgcttcaa ttcctgtctt tcttaggtag tcattggcca gctggcgctg
      421 agccttgctg taacgaccgg taagcccttt ttgggataga agtccggaaa gaactacctc
      481 cataacggtt atgggaaaat ttttatcgag catacctgcc tgtggcaggt aaccaattgg
      541 aagttgtttt ccactggtag agtagcttac ctcaccgctg aagggtttta cttgccctat
      601 cagcagcttc aggaaagttg tcttgccccc gccattcggc ccaattatcc caataaaatc
      661 gtcgtcgtag atgtcgaggc tgacattttt caacaccacg tcacgcccat acccggcggt
      721 aacattccta atctctacta ttcttgaatg ttcgttactc atcatgctcg cctgaaattg
      781 cccttgctat atgcaacatg ctgttttccc agtcttccgc tagcggatca accttaacaa
      841 cccttaggtt aagctcatcg gctatgacct gggcattgcg aatatcgaac tggctttggg
      901 aaagaacggc cgatacgcct aacctgcgag cctggtcaac aacatcgacc acacccttaa
      961 gcgatggatt ttttccctcc tgctcaatgc taatttgcct gaggccaaac tcccgtgcat
     1021 agtaacccag tgcaggatgg taaaccaaaa atgcacgatt ggaagatttt ccaagtatcc
     1081 tgttaacctg ttccttcagg ctatccagct tgctgccaaa agcaatgtag ttgttgctaa
     1141 aaaagtcgct atgggcagga taaagctgct ggaggttctg taggatttgc atggacatct
     1201 tccgcacctc ctcaaccgac atccagatat gggggtcaac cccatgatgg tgcccgcctg
     1261 cagaatcatg ctcgtcggtt ccttccttta tcaggtcagc ttttcccgat aggtttacat
     1321 accttacttt cagctggtcc tttagtttag gttccagacc tttttcaaaa tcgagaagcc
     1381 cgatggcaaa gtatatgtcg gcgttactta aatcgaccat ttgtcccggg gttggctcaa
     1441 acatttcggg gctgctgccc ggaggaacca ttaccctaac atccaccagc gtgtcggcaa
     1501 tttgctcaac aaagtactta atgggttgaa tgctgaccat tacaataggc ttgctggtgg
     1561 tatgcttctt ttctgtgcag gccggtagca ttaaccctaa taccacgacc aaaaaaacca
     1621 tgattccact aatccttcgc atttcttgtt aatttagcat ctacacttcg ctaccctgct
     1681 tccaggttcg gttgggctga acggaccggt tgagacctac cggattgttt tgaagatgac
     1741 cagaacccgt gtaaacatca aacaaaccca aattaagcaa atttgtttac cagacatctt
     1801 ctttttaaaa gttttgcatt ttggttaaac ttttgttaat ttgccagtta aagc
//
LOCUS       DAOOQX010000432         1853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_79421_length_1853_cov_3.108454, whole genome shotgun sequence.
ACCESSION   DAOOQX010000432 DAOOQX010000000
VERSION     DAOOQX010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79421_length_1853_cov_3.108454"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="PLX53_07120"
     CDS             complement(<1..116)
                     /locus_tag="PLX53_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72890.1"
                     /translation="MSMFCFQCQETAKGTGCTIKGVCGKSDDLANMMDLLMFV"
     gene            complement(256..1179)
                     /locus_tag="PLX53_07125"
     CDS             complement(256..1179)
                     /locus_tag="PLX53_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOY72891.1"
                     /translation="MKRDIITIDREKCNGCGQCVGGCHEGALQLIDGKAVLVSELMCD
                     GLGACIGECPEGAITVERREADAYDEVTTLSQMVASGKNTTVAHLKHLKEYHQTDYLR
                     QAVEWLLSNRKNVSFSVDEVMQEVHNHSGSSCGCGNGGKHEPTQQSHHGKHVDGCPGS
                     AERSFGNNIRVEASKMVSGKSELTHWPVQLHLINPHSEHFKGSNLLLAADCVAYSQGS
                     FHSQHLAGKTLAIACPKLDSNKEEYIEKITAFIDDAQVDTITVMKMEVPCCGGLLQLT
                     RLARDKARRSVPIKVITVGIQGDILDSVWEN"
     gene            1439..>1853
                     /locus_tag="PLX53_07130"
     CDS             1439..>1853
                     /locus_tag="PLX53_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOY72892.1"
                     /translation="MVQVEEKPVQWVAGQNMANLKCIDNAFLLMENGLIKDFGSMRDV
                     PSFAGLPSDVLIYNVNGRMVFPSFCDSHTHLVYAGSREVEYIDKIKGLSYEEIARRGG
                     GILNSAKLLSQTSEDELYEQALPRVKEIIRKGTGAV"
BASE COUNT          466 a          447 c          414 g          526 t
ORIGIN      
        1 acaaacatta gtaggtccat catgttggca aggtcatcgc tcttaccgca tacccccttg
       61 attgtacagc cggttccttt ggctgtctcc tgacactgaa aacaaaacat gctcataatt
      121 caaaattttt aaattggtta gatattgttt gtaagtagta gctgtgcagc ttgtttataa
      181 ataggttgat ggggaaaacc ctcgagtcag gtaaatttaa aatattttat tcttgactct
      241 agttttttct ccatcttagt tttcccaaac ggaatccaga atatcaccct gtatgccaac
      301 ggttattacc tttatgggca cactacgcct ggccttatcg cgggcaagcc gggtaagctg
      361 gagcaacccg ccgcagcagg gaacctccat cttcatcact gtgatggtgt ccacttgggc
      421 atcgtcaata aacgcggtaa tcttctcaat gtactcctcc ttgttggagt caagcttagg
      481 acatgcaatg gccagcgttt ttccggcaag atgctggctg tggaagctcc cctgcgagta
      541 ggctacacaa tcggctgcga gcagcaggtt cgacccctta aagtgttccg aatgcggatt
      601 tatcagatgg agctgaaccg gccaatgggt tagctccgac ttgccggata ccatttttga
      661 tgcctcaaca cgtatattat ttccaaaact cctttctgct gaaccggggc atccatccac
      721 atgtttacca tggtgggact gttgtgtcgg ttcatgtttc cctccatttc cgcaaccaca
      781 gctgctgccg gagtggttat gaacctcctg catcacctca tcaacactaa aggaaacatt
      841 tttacgattt gaaagcagcc actctacagc ttgtcgtaaa tagtcggttt ggtggtattc
      901 tttaaggtgc tttagatggg caactgtggt atttttgccg ctggctacca tttggctgag
      961 ggtggttacc tcatcgtagg catcggcctc gcgccgctca acggtgatgg ctccctcggg
     1021 gcattcacca atgcaggctc ccagcccatc gcacatcagc tcgctaacta aaactgcctt
     1081 gccgtcaatc agctgcaggg caccctcgtg gcatccacct acacattggc cgcaaccgtt
     1141 acatttttcc ctgtcaattg taatgatatc tcttttcatg cctgcttttt tacgaaaaaa
     1201 aatttcaacc tttttctgga gcaaagctaa ggcaccaccc cctcctaatt atgtaacaaa
     1261 tgttacaaaa cgagtaaaat ttttaatacc agctcaaaaa cactaggatg ctggctggtg
     1321 cagggcttta acgcatacct tttattaaat aatatttagt tcttcccaat tttattgcta
     1381 ccttcgtcgg agatttatgc tgaaaaccat gcaaatactg ctacttaaca taaaaaagtt
     1441 ggtacaggtc gaggaaaagc ctgttcagtg ggttgcgggc caaaatatgg ccaacctgaa
     1501 gtgtattgat aacgcattcc tgctgatgga gaatggttta atcaaggatt ttgggtctat
     1561 gagggatgtt ccttcatttg ccgggctacc cagcgatgtg ctgatttaca acgtaaatgg
     1621 gcggatggtt tttccctcct tctgcgactc tcacacccat ctggtatatg ccggcagccg
     1681 cgaagttgag tatattgata agataaaagg gctatcgtac gaggaaattg ccaggcgtgg
     1741 cgggggaatt cttaactccg caaagctact ctcccaaact tccgaggacg agctgtacga
     1801 gcaggcattg cctagggtta aagaaattat ccgcaaggga acaggagctg ttg
//
LOCUS       DAOOQX010000433         1852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_79501_length_1852_cov_2.543127, whole genome shotgun sequence.
ACCESSION   DAOOQX010000433 DAOOQX010000000
VERSION     DAOOQX010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79501_length_1852_cov_2.543127"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PLX53_07135"
     CDS             <1..1018
                     /locus_tag="PLX53_07135"
                     /inference="COORDINATES: protein motif:HMM:NF025125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOY72893.1"
                     /translation="EFGVKEILYRIDSSKFEAYRYPIKAGSLTDGDHVLEWYATDMVG
                     NSEALQSHHFTVDKTPPIVVEEIAGNTYMVGNVEYSSGRSQLRIVAIDNRAGIKDIFY
                     SLNNGEYIRYEKQVMLSDILGTINVKTYAVDNVNNRSQSGANTESFVMPTIDITGPTI
                     GYQLLGRQLTLRDTLWIGQSTLVKLNAADKESGANRILYTIDKSGGMDYTGPFSLTEP
                     GFHEIRCTAYDNVDNLNFLSFKLGLDSEAPKIYHHFSVEPFKELPVGNVPVYPASVKL
                     YLAATDNLTGGVSVKYSVNEGKLTDYTQPIENFKPGNNYTLSITATDELGNSSTQTIN
                     FRVE"
     gene            1462..>1852
                     /locus_tag="PLX53_07140"
     CDS             1462..>1852
                     /locus_tag="PLX53_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72894.1"
                     /translation="MNLHRYTIKKLLSFTSTIILLSFLLFTSIYYIRQQAVRQDKNIL
                     QRLDRLEQNSLRIKMLQDEFLLRDFHNSQLYVLGQSPATLRLDSLFTKTLEELNYLKK
                     YFSSTNITEVERHIEQYKKNFNNLKEME"
BASE COUNT          602 a          458 c          366 g          426 t
ORIGIN      
        1 tgagttcggt gtgaaagaaa tcctctaccg gattgactcg tcaaaatttg aagcctaccg
       61 gtatccgatc aaggcaggaa gtctgactga cggagaccac gttttggaat ggtacgccac
      121 cgacatggtg ggtaatagcg aagccctgca gagccaccat ttcacagtgg acaaaacacc
      181 accaattgta gtggaggaaa ttgccggcaa cacctacatg gtgggcaatg tggagtactc
      241 atcgggtcga agccagctta gaattgttgc cattgacaac agagccggta ttaaggatat
      301 tttttactcc cttaacaatg gcgagtacat tcgctatgaa aaacaggtaa tgctctcgga
      361 tatacttgga accataaatg tgaaaaccta cgcggttgac aatgtaaaca accgcagcca
      421 gtcaggagca aacaccgaat cgttcgtaat gcccaccatt gacataaccg gcccaaccat
      481 tggttaccag ctgctgggcc gccagctaac cctccgcgac accctttgga ttggacaatc
      541 aacgctggta aagctaaatg ctgccgacaa ggagtcagga gcaaaccgca tcttatacac
      601 cattgacaaa tcgggtggca tggactacac cggcccattt agcctgaccg aacccggatt
      661 ccacgaaatt cgatgcaccg cctatgacaa cgtggataat cttaacttcc tatcgttcaa
      721 actagggctt gatagcgaag ctcccaaaat ctaccatcac tttagcgttg aaccctttaa
      781 agagctacct gttggtaatg ttcccgttta ccccgcaagc gtaaagcttt acctggctgc
      841 aaccgataac ctgacgggag gtgtttccgt aaagtattcg gttaatgagg gcaaactaac
      901 cgactacacc caaccgattg agaacttcaa acccgggaat aactatacct tatccatcac
      961 agccaccgat gagctgggaa acagctcaac acaaaccatc aactttaggg tggagtaggt
     1021 tccaaccgga gagacttgcc cgaaaacaca gcatgaatag caacaccggg gagaatttca
     1081 ttttcctcga ccaaagccct tccttcggat tgccataaac atggccggat ttcccttctg
     1141 ggagtcccct ctctagcgca attgctacca accagccgct tctggaacct tgttcaatat
     1201 aagaaaatcg ctactgacag gtaagcatat agcttactga taaaaaacag cagaaaaaat
     1261 atcaccactt atgaaattta aaattgcata gctataccct tatttaacca ggcaaaaaat
     1321 tgcgtagcag tgctccagct actgctttac aaaaggtgta acaataacaa cattaaccat
     1381 ttaaacaatt aacacataat taattgaatg gaaaccttga atatataaat aattggaaaa
     1441 tttgaagaaa aataagtagc catgaatcta cacaggtaca ccataaaaaa actgctctcc
     1501 ttcacctcaa ccatcatcct cctttcattt cttcttttca cctctattta ctacattcga
     1561 cagcaagcag tgcggcaaga taaaaacatt cttcaaaggc tagatcggct agaacaaaac
     1621 agcctgagaa taaaaatgct gcaggatgaa tttttgctac gcgacttcca caacagccag
     1681 ctttacgtgc taggacaaag cccggcaacg ctgaggcttg acagcctgtt tacaaaaacc
     1741 ctcgaagaac tcaactacct aaaaaaatat tttagttcga caaatattac cgaggttgag
     1801 cggcacattg agcagtacaa aaagaacttc aacaacctga aagagatgga ga
//
LOCUS       DAOOQX010000434         1850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_79606_length_1850_cov_3.907521, whole genome shotgun sequence.
ACCESSION   DAOOQX010000434 DAOOQX010000000
VERSION     DAOOQX010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79606_length_1850_cov_3.907521"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1063
                     /locus_tag="PLX53_07145"
     CDS             170..1063
                     /locus_tag="PLX53_07145"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOY72895.1"
                     /translation="MKPNLTAQSTNKLIDEKDITKLSRFFKGKAGEKFLKFLMHLLAI
                     DKVNWVYDHSFDYSGPEFTSRLLSDIGVDYKVGNPERLEKLPKGAFITIANHPYGGLD
                     GIMLIDILARIRPDFKIMANKQLSLIKTLASNFISVLPKTDANKGYSPFNFQSIRDTL
                     NLIRSGHAVGFFPSGAISDFKFRNLRVSDREWQDSIIRLIQKAQVPILPIRFFDRNSI
                     FFYFLGLIDWRIRSLRLPHEIFNKQGQCQRIGVGEIITVGEQAKYPDSEMFGMFLRNM
                     VYGMPLPSAFIQKSALCLPTK"
     gene            1258..1398
                     /locus_tag="PLX53_07150"
     CDS             1258..1398
                     /locus_tag="PLX53_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72896.1"
                     /translation="MWASSEIVLGSFLHNLKIPFSGSIPTAIGLVILISASYKWREKG
                     IL"
     gene            complement(1648..>1850)
                     /locus_tag="PLX53_07155"
     CDS             complement(1648..>1850)
                     /locus_tag="PLX53_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72897.1"
                     /translation="TTYDYHLKEFLSSYSSLRVEVNNPLVLANINTIDDYRQYFGDWK
                     LSKYQAPIQNSYDGFDVKKLTE"
BASE COUNT          529 a          413 c          380 g          528 t
ORIGIN      
        1 tggggctgta ctcctagaaa tcgtattcat caaccaatga gcaaggctgt attgcccaaa
       61 tatcaggctg agtcgaaagt taagcggaag tcttacacac tcgttgtcca ttttccaaat
      121 gcagataata tgtgtatttt tgttttcctc taaatgattt tgttctaaaa tgaagcctaa
      181 cctcaccgcc cagtcgacta ataagctcat tgacgaaaaa gacattacta aactatccag
      241 atttttcaag ggtaaagctg gagaaaagtt cttgaaattc ttaatgcacc tgcttgcaat
      301 tgataaggtt aactgggttt atgatcactc cttcgactac tcagggccag agtttaccag
      361 caggctgctt agcgacattg gggttgacta caaggttggt aaccccgaac ggcttgaaaa
      421 attgcccaaa ggagctttca tcaccattgc caaccatcca tatggaggcc tggatggaat
      481 tatgctgatt gatattttgg caagaattcg tcccgatttt aagattatgg ccaacaagca
      541 gctttcgcta atcaaaacac tagcctcaaa ctttatttcc gttcttccta aaacagatgc
      601 aaataagggc tactcaccgt ttaactttca gagtattcgc gacacgttga acttaattag
      661 aagtggccat gctgtgggtt ttttcccttc gggggctatt tccgatttca agttccgtaa
      721 ccttcgagta agcgaccgcg agtggcaaga tagcattatc cgcttaatac aaaaggcaca
      781 ggttccaatc ttacccatac gtttttttga tcgtaattcg attttcttct actttcttgg
      841 gctgattgac tggcgaattc gtagcttaag gctaccccac gaaatattta ataagcaggg
      901 tcagtgccaa cgcatcgggg ttggagaaat aattactgtt ggcgaacaag ccaaataccc
      961 cgactccgaa atgttcggca tgtttcttcg aaacatggtg tacggtatgc ctttaccctc
     1021 agcatttata caaaaatcag cgctatgtct tcctacaaag taggactgca atcaatttta
     1081 tttatcaacc caataggcaa ttcaatagta atggcagctt ggctggttaa aagataaaaa
     1141 tttcattaca gcatggccca aagggaaaaa tccgccactt ttttagctag cagcccgcta
     1201 caaaacactt ccatacagct aagtatcaag tgagttaggg ctttattgct tggcacaatg
     1261 tgggcatcat cggaaatagt tctaggtagc ttcctgcata acctaaaaat tccgttcagt
     1321 ggtagcatcc ccacagccat cgggctggta atcctcattt cggccagcta caaatggcgc
     1381 gaaaagggaa tattatgacg tgcagtcgtt atatgcgccg taataaaaat ccgatcaccc
     1441 agtgctgtta ttatcgggcc aatggttgcc attttcaacg aggcggctct gctggaaatc
     1501 tcagtgcaaa tcccaggaca caccatccca gcgtttgtac tagcaccctg cccacatccc
     1561 attttcaccc gtggggcatc agaattaaca tgtttatttg tgaagtaatg taaaaggatg
     1621 gggcaactgg aataaccttt aggctgccta ttccgttaac tttttcacat caaaaccatc
     1681 ataagaattt tggattggtg cttggtattt gctcagcttc caatcaccaa aatactgacg
     1741 gtaatcgtcg atggtattga tattagccag aacaagcgga ttgttcacct caacccgcaa
     1801 gctagaatag cttgaaagga actcctttag gtggtagtcg taagttgtgc
//
LOCUS       DAOOQX010000435         1840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_80323_length_1840_cov_2.264986, whole genome shotgun sequence.
ACCESSION   DAOOQX010000435 DAOOQX010000000
VERSION     DAOOQX010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80323_length_1840_cov_2.264986"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..>1840
                     /locus_tag="PLX53_07160"
     CDS             159..>1840
                     /locus_tag="PLX53_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY72898.1"
                     /translation="MSLSDTIRMDEVKVYGNLRESHSIGATIYRMDSVKLKIFQNRSA
                     AELLAMGSVNLKSYGVGGLSSLTMRGGNSSQTIVVWNGVNIQNPMNGGANLSLFPVNM
                     FSSIRLQYGGSGTIYGSGAISGILILNSNALFPQPNGGELSLSYGSGNTRSLSSQVKY
                     GDKSRAISVKVFGQLADNDFEFRNIYKIGSPKERITHAESKQLGILTDVNMRLHEKLF
                     WSFSGWGQLGDKNLQPIMSSTQPSYANQVDRSLALVSNLSLSPSKRILFNLKNAASLG
                     TIDYSDKGVNIFTNNSYASILTELESKVDVLNNGEVSMGINHTLETASSDDYVGSAKR
                     NRVSGYASFRKPLFSSRFLSVLSMRGELVDGDFKPVIYSVGAEWLPTKKLIFKMNASK
                     NYRIPTLNDLYWASTTYTEGNPNLEPESGWGGDVGFQVKSISSDRAINVSNSLFYSNI
                     ERWIVWLSIPTVSDSKWKPFNMSKGESWGWESQVDWSLRVNSLTIRVEGFYTFTHSVF
                     SEQPGSRAQPMIYTPTHRFSGSLNLQYEKYGLGYYHGFTGKRYRDFQSQLPA"
BASE COUNT          505 a          387 c          438 g          510 t
ORIGIN      
        1 cgaagattag aacgctgttc cattcagttc tacttttcac tatttccgag tgctgcgaat
       61 gattgattgc ctattaatca tttgtagcta tgcatcttaa aagggtttgc tcgatcgttt
      121 ttcttcagct tgtgctggtc ggttatttgt gtgctcaaat gtcgttatcc gacaccattc
      181 gcatggacga agtgaaggtt tacggcaatt tacgcgaaag ccattctata ggtgccacca
      241 tttatagaat ggattctgta aagctgaaaa tctttcaaaa tcgatcagct gccgagttgc
      301 tggcaatggg ttcggtgaac ctcaaatcgt acggtgttgg cggcctttca tcgctaacca
      361 tgcggggcgg aaactcctca caaactatcg tggtttggaa tggggtgaac attcaaaacc
      421 cgatgaatgg gggtgctaac ctttcgctat tccctgtaaa catgttcagc agcatcagat
      481 tgcagtatgg cgggtcagga accatttatg ggagcggggc catatcggga atactaattt
      541 tgaacagcaa tgcattattt cctcagccta atggtgggga gctatccctt tcatacggaa
      601 gcggaaatac ccgcagcctt tcatcacagg ttaagtatgg cgacaaaagc cgggcgattt
      661 cggttaaggt ttttggacaa ttagctgata atgacttcga gtttcgtaat atttacaaaa
      721 ttggcagccc aaaggaacgc attacacatg ctgagtcgaa acagctggga attttgaccg
      781 atgtcaacat gcggctgcat gaaaaattat tttggtcgtt ctcgggatgg ggccagctag
      841 gcgacaaaaa tctgcaaccc attatgagca gcacgcagcc cagctacgcc aaccaggttg
      901 accgaagcct ggcgttggtt tccaacctta gcttatcacc ttctaaaagg attcttttca
      961 acctgaaaaa tgctgcaagt ttgggaacta tagactactc agataagggc gttaatattt
     1021 ttaccaacaa cagctacgcc tctattctca ctgagctgga atcaaaagtg gatgttctga
     1081 ataatgggga ggtaagcatg gggattaacc acaccctcga aacagccagt tccgacgatt
     1141 atgtaggaag tgcaaaaaga aacagagttt caggatatgc ctcgtttcgt aagcctttgt
     1201 ttagctcaag atttttatca gtgctgagca tgcgtggtga gttggttgat ggtgatttca
     1261 aacctgtcat ctattctgta ggagcggaat ggcttcccac aaaaaagtta atctttaaaa
     1321 tgaatgcctc caaaaattat cgaataccta cacttaacga cctctactgg gccagcacaa
     1381 catataccga gggaaatccc aatcttgaac ctgaatcggg ttggggtggc gatgtgggat
     1441 ttcaggtaaa gagcatcagc agcgataggg caataaacgt ttcaaattcg ctattctaca
     1501 gcaatattga gcgttggatt gtatggctca gcattcctac ggtttccgat agtaaatgga
     1561 agcctttcaa catgagcaaa ggcgaatcgt ggggttggga gtctcaggtc gattggagtt
     1621 taagggttaa cagccttact attcgtgtgg aaggatttta cacttttacc cattccgtat
     1681 tcagtgagca accgggtagt agggctcaac caatgattta cacaccaacc cacaggtttt
     1741 ccggctctct taacctacag tatgaaaagt atgggctggg atactatcac ggttttaccg
     1801 gcaaacgcta tagggatttt caaagccagt tgccggcatt
//
LOCUS       DAOOQX010000436         1840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_80336_length_1840_cov_1.783193, whole genome shotgun sequence.
ACCESSION   DAOOQX010000436 DAOOQX010000000
VERSION     DAOOQX010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80336_length_1840_cov_1.783193"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /gene="rplT"
                     /locus_tag="PLX53_07165"
     CDS             complement(<1..225)
                     /gene="rplT"
                     /locus_tag="PLX53_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOY72899.1"
                     /translation="MPRSVNAVASRKRRKKILKQTKGNFLARRNVYTVAKNTLEKGLT
                     YAYRDRKKKKIEFRALWIQRINAAVRPYGLT"
     gene            complement(334..531)
                     /gene="rpmI"
                     /locus_tag="PLX53_07170"
     CDS             complement(334..531)
                     /gene="rpmI"
                     /locus_tag="PLX53_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOY72900.1"
                     /translation="MPKIKTHSGAKKRFSLTGTGKIKRKHAYKSHILTKKETKQKRNL
                     THFTLVDKADEKRVKKMLGLR"
     gene            complement(558..1112)
                     /gene="infC"
                     /locus_tag="PLX53_07175"
     CDS             complement(558..1112)
                     /gene="infC"
                     /locus_tag="PLX53_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOY72901.1"
                     /translation="MATPLHSQVKKDVDNKFLVNNEIRNKVVRLVGENIENPGIFSIE
                     EARRLANELELDLVMISDKADPPVCRIIDYQKFLYQQKKKQKEIKANAQKIVVKEIRF
                     GPNTDEHDYNFKLKHAQNFLKEGAKVKAYVFFKGRSILFKEQGEILLLRFAQDLEDLG
                     KVEQLPRLEGKRMIMLISPKGAKK"
     gene            complement(1142..>1840)
                     /locus_tag="PLX53_07180"
     CDS             complement(1142..>1840)
                     /locus_tag="PLX53_07180"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOY72902.1"
                     /translation="NFKEYTAQVSLRDPKDREKYIGTDENWEKAESAIIEAAAERGLK
                     TTTVVGEAAFYGPKLDFMVNDAIGRKWQLGTIQVDYNLPERFQLEYIGSDDKRYRPVM
                     IHRAPFGSMERFVAVLLEHTGGRLPIWLAPDQVVVLPISEKYSDYAKKVFNYLNNSDI
                     RTLIDDRNEKIGKKIRDNELRHIPYLLIVGEKEEENGTVAVRVQGEGDKGQMSLDDFI
                     MLVRNNIESQLTEI"
BASE COUNT          447 a          428 c          366 g          599 t
ORIGIN      
        1 ggttaaccca taaggcctaa cagcagcgtt aatacgctga atccacaaag cgcgaaactc
       61 aattttcttc ttctttcgat cccgataggc ataggttaaa cctttctcaa gggtgttctt
      121 agcaacggtg taaacgttgc gacgggcaag aaaattaccc ttggtctgct tcagaatttt
      181 tttccttctc tttcttgatg caacagcatt gactgaacgt ggcatagttt taaaattttt
      241 gaatggttag cgttccgcaa cccggacact taggcgctaa ttcattctgg ttagtaatta
      301 aaagtagctg taaataaaag gctaaagtca tggctacctg agcccaagca tttttttgac
      361 ccttttctca tctgccttgt caacaagggt aaagtgggta aggtttcttt tttgtttggt
      421 ttccttcttg gtaaggatat ggctcttgta agcatgtttc cttttaattt ttcctgttcc
      481 ggtcaaagaa aaccttttct tggcaccgga atgagtctta attttcggca ttctaaactg
      541 aaattattta ggtttattta cttctttgct ccttttggag atattaacat gatcattcgc
      601 ttgccttcca acctgggtag ctgctcaacc tttccaaggt cttctaaatc ctgggcaaat
      661 cgtaggagta atatttcgcc ctgctcctta aaaagtattg aacgtccctt aaaaaatacg
      721 taggctttca cttttgcccc ttctttaagg aaattctggg catgtttcag cttaaagtta
      781 tagtcatgct catcggtatt tgggccaaaa cgaatttcct taacaacgat tttctgtgca
      841 ttagccttga tttccttctg tttctttttt tgctggtaga gaaacttctg gtaatcaata
      901 atgcggcaca ccggtggatc ggccttatca gaaatcatca caaggtctaa ctccagctcg
      961 ttagccagcc gcctagcctc ttcgatggaa aaaatacccg ggttctcgat attttccccg
     1021 acaagccgca ctaccttgtt tcttatttca ttgtttacca ggaatttatt atctacgtcc
     1081 tttttaacct gggaatgtaa tggtgttgct atagctttgt cctcctattg gttatagtta
     1141 actatatttc agtaagctga ctttcaatat tattcctaac cagcataata aaatcatcga
     1201 gggacatttg tcctttatcg ccttcacctt gaacccgaac ggcaaccgtg ccgttttcct
     1261 cctccttttc gcccacaata agcaggtagg gaatatggcg taactcgttg tcccttatct
     1321 ttttaccaat tttctcgtta cggtcgtcaa tcagggtgcg aatatcggaa ttatttagat
     1381 aattaaaaac tttttttgcg taatcgctgt acttttcgct gatgggcagc actacaacct
     1441 ggtcgggtgc aagccaaata gggagcctcc ctccggtatg ctcaagaagc acagccacaa
     1501 agcgttccat tgagccaaat ggggcacgat gtatcatcac cgggcggtag cgcttgtcgt
     1561 cggagccaat gtactccagc tgaaagcgct caggcaggtt gtagtcaacc tgaatagtcc
     1621 ccagctgcca ttttcggcca atggcgtcgt taaccatgaa atcaagctta ggtccgtaga
     1681 atgccgcctc gccaacaacg gtggtagttt taagtccccg ttcggcagca gcctcaataa
     1741 tggcgctttc ggctttttcc cagttctcgt ccgttccaat gtatttttcc ctatcctttg
     1801 ggtcacgaag tgaaacctgt gcggtgtact ccttaaaatt
//
LOCUS       DAOOQX010000437         1839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_80364_length_1839_cov_3.367713, whole genome shotgun sequence.
ACCESSION   DAOOQX010000437 DAOOQX010000000
VERSION     DAOOQX010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80364_length_1839_cov_3.367713"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLX53_07185"
     CDS             complement(<1..421)
                     /locus_tag="PLX53_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOY72903.1"
                     /translation="MFTPGNISISDFEYSLPTDRIASHPLSKRDESKLLIYRDGEIGS
                     DVFKNIGNYLPEGSRLVLNNTKVVSARIIFLKETGATIEVFLLEPVFPADYSVSFSSH
                     EPVVWKCMVGNQKRWKTDTLERTVPQTDTKLKVRKSKK"
     gene            547..1623
                     /locus_tag="PLX53_07190"
     CDS             547..1623
                     /locus_tag="PLX53_07190"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HOY72904.1"
                     /translation="MKVEVGDRVRFLNEVGGGKVLRILDKRMVLVETDDGFEIPVLAA
                     EVVVINRNADSQIIGDDSLPMPTLPKQTDKHPKESNQKAGSAAGISMQGATERQGDDI
                     MLLLGFVPDSLYTLASSGLKLFLINDSSYKIFYTLSSWNEGKLNPIISGLLEADTKEQ
                     VAYLVNDEARSAKTLNLQAIYHKGMPFKAYQPEFYDVEIASLKLQLAASYVENDYFDE
                     KACLVTIADTQKEVLLERMDRESIQGIIQEKENQHVKNKPKKSLPVNEIEEVDLHNEE
                     LVDSWSNLSPGEILNIQLARFETALQGGIKSNTKRMVFIHGVGNGKLKYEIHKLLDTR
                     FAGLKYQDASFKEYGFGATLVYLK"
     gene            1639..>1839
                     /gene="rnpB"
                     /locus_tag="PLX53_07195"
     ncRNA           1639..>1839
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLX53_07195"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          556 a          348 c          417 g          518 t
ORIGIN      
        1 cctttttcga ttttctcacc tttaatttag tgtcagtttg aggtactgtt cgctctagcg
       61 tatctgtttt ccatcgcttt tgatttccaa ccatgcattt ccatactact ggttcgtggc
      121 ttgaaaagga aaccgaataa tctgccggga atacaggttc tagtaagaaa acctcaattg
      181 tagccccggt ttctttcagg aagattatcc gggcactaac taccttggtg ttatttaaaa
      241 ctaaacgact cccttcagga aggtagttgc ctatattttt gaaaacatcg cttccaattt
      301 ccccatccct gtatatcaac aacttcgact catccctttt acttaatggg tggctggcta
      361 ttcggtcggt tggtaagcta tactcaaaat cggaaatgct aatattgcct ggagtaaaca
      421 tcacattaga attcaaactc acgctgttgt atccctattc ccttgaaatg gcaaaattag
      481 ctaattttgc agttctttta actttaatgg attgggttaa atcatgagtt taaaaataaa
      541 tagagtatga aagttgaggt tggagatagg gtgcgttttc tgaatgaggt tggcgggggt
      601 aaggttctaa ggattcttga taagcgaatg gttttggttg aaactgatga cgggtttgaa
      661 ataccggttc tggcagcaga agttgttgtt attaaccgga atgccgacag tcagattatt
      721 ggtgatgatt cattaccaat gcccactctt cccaaacaaa cagataagca tcctaaagaa
      781 agcaatcaaa aggcaggtag tgcagcaggc ataagcatgc aaggagcaac ggagagacag
      841 ggtgatgata taatgctttt acttgggttt gttcctgata gcctatacac gcttgcaagc
      901 agtggcttaa agttgtttct aattaacgac agcagctata agatttttta caccttatca
      961 tcatggaatg aaggaaagct aaatccaatt atttcgggat tgcttgaagc cgatactaaa
     1021 gaacaggttg cataccttgt aaacgatgag gctagaagcg cgaaaacgtt gaacttgcag
     1081 gctatttacc acaaaggaat gccatttaaa gcctatcagc cagaattcta cgatgttgag
     1141 atagcctcgc tcaagcttca acttgcagcc agctacgtgg aaaatgatta tttcgatgaa
     1201 aaagcctgtc ttgttactat tgcagatacc caaaaagagg tgctgttgga aagaatggat
     1261 agggaatcta tacagggaat catacaagag aaggaaaacc agcacgttaa gaataagcct
     1321 aaaaaatcac ttccggtcaa tgaaattgaa gaggttgatt tgcataatga agagctagtt
     1381 gatagctgga gtaacctttc tccaggagaa attcttaata ttcagcttgc ccgctttgaa
     1441 acagcgcttc agggaggcat taagtccaac accaaaagaa tggtgttcat tcacggcgtt
     1501 ggtaacggca agctgaagta cgaaattcac aagctacttg acaccaggtt tgctggttta
     1561 aaatatcagg atgcctcatt taaagagtat ggatttggtg caaccttggt ttacctaaag
     1621 tagtataatc ttctatagca agttatccgc tctatcgctt cttgaaatac aggagaggaa
     1681 agtccgggca gcaaagggtg ccatgcttcc taacaggaag atgctcgtga gggcatagta
     1741 gtgtaacaga aaataaccgc tctgctgctg gcagggtaag ggtgaaaagg tggggtaaga
     1801 gcccaccggg ttccgtagca atacggaatg ccgtacacc
//
LOCUS       DAOOQX010000438         1828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_81169_length_1828_cov_2.566836, whole genome shotgun sequence.
ACCESSION   DAOOQX010000438 DAOOQX010000000
VERSION     DAOOQX010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81169_length_1828_cov_2.566836"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLX53_07200"
     CDS             complement(<1..418)
                     /locus_tag="PLX53_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HOY72905.1"
                     /translation="MRILVTGANGLVGKKITRQLLSGGKHEVYATSLKKLQIPGAVTF
                     TSNLLNADINFMVDETHPDVIIHCAALSSPDACEVDRFACKRLNVDLTARIAMACNDY
                     GVQLILMSTDFVFDGLKGQYAEEDRANPICYYGMSKA"
     gene            complement(524..>1828)
                     /locus_tag="PLX53_07205"
     CDS             complement(524..>1828)
                     /locus_tag="PLX53_07205"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOY72906.1"
                     /translation="CHKKQLDTMVESFIHFLWQYKSFAFSGLNTSDNCQLEVLDPGTY
                     NSDAGPDFFNAKVRLNGTTWVGNVEMHINSSEWFAHNHHTDRAYDNVVLHVVVNNDKP
                     AINSRGVPIPTLAISYPFGLEREYSRLTSSGSYIPCGEVLPNVDNLTKRALLDRMMVE
                     RLQAKTDEIGKSLEKCHGSWEEAFYRAVTRTFGLKVNAFPAEILAQETPLKVLAKHKN
                     NLFQLESLIFGQAGLLNRFDVEDEYLLSLRMEYSFLKKKFNLTPIDGSLWKFLRMRPV
                     SFPTIRLAQLAMLIHHSTSLFSKAVEASSLKQLQELFQVETSTYWYNHYLFGFPVKSR
                     KKSLGTATINVIIINAVIPFMFAYGGWRGNQKLKEKAISILEEIPPEENHIIKKFDSM
                     GVKSCNSSDSQAVIHLKSTFCDARKCIFCPVGIKLLLKAAYI"
BASE COUNT          525 a          390 c          381 g          532 t
ORIGIN      
        1 cagcttttga catcccgtag tagcagattg gattagcccg atcctcttcc gcatattgac
       61 ctttcaatcc gtcaaaaaca aaatcggtgg acatcagaat aagttgaact ccatagtcat
      121 tgcaagccat ggctattcgg gcggtgagat ctacatttag ccttttacag gcaaaacgat
      181 caacttcaca tgcatcggga ctggaaagtg cagcacaatg tataataaca tcgggatggg
      241 tttcatcaac cataaagtta atgtctgcat ttagcaggtt tgatgtaaag gtaacagcgc
      301 cgggaatttg cagctttttt agggatgtag cataaacctc atgctttccg cctgaaagta
      361 gctgccgggt aatttttttc ccaacaagtc cgtttgcccc agtaaccagt attctcatat
      421 tgcaattaaa tacgtgtatt ttctttaact ccagcaattc cccgatttcc gcaaatgtta
      481 taaagtttgt tgtagggtgt atttttacgt tcgttttaag actctaaata taggctgctt
      541 taagcaataa ttttatgcca acagggcaaa atatgcactt tcgagcatcg cagaatgttg
      601 actttaagtg tataacagcc tggctatcag acgaattaca tgacttaacc cccatagaat
      661 cgaatttttt tataatatga ttttcctcgg ggggaatctc ctccaaaatt gaaattgcct
      721 tttcctttag tttctggtta cccctccaac caccataggc aaacatgaag ggaatgacgg
      781 cattaataat aatcacgttg atagtagcag tgcccaaact tttttttcgt gatttaaccg
      841 gaaatccaaa taagtaatga ttataccagt aagtcgatgt ttcaacttgg aaaagttctt
      901 gcagctgctt taaggagctt gcctccaccg ctttggaaaa aagagaggta gaatgatgta
      961 ttaacatggc aagctgcgca aggcggatag tgggaaagga aacaggcctc attcgtaaaa
     1021 atttccatag gctaccatcg atcggagtaa ggttaaattt tttcttaaga aaggagtact
     1081 ccatcctgag cgaaagtagg tactcgtcct ctacatcaaa acggtttagt aatccggcct
     1141 gcccaaatat aagcgattcg agttgaaaaa ggttattctt atgctttgcc agcaccttta
     1201 aaggggtttc ctgggctaag atttcggccg gaaaagcgtt gaccttaagg ccaaaggttc
     1261 tggtgaccgc ccgatagaaa gcttcctccc agcttccatg acatttttcg agcgatttac
     1321 ctatttcatc ggttttggcc tgtaaacgct ccaccatcat tctgtcgagg agggctcttt
     1381 ttgttaggtt atcaacattt ggtagcacct caccgcatgg gatgtagctt ccagaactcg
     1441 tcaatcttga atattctctt tccaacccga acgggtagct tatggcaaga gtgggaatag
     1501 gcacaccgcg ggaatttata gccggcttgt cgttgttaac aaccacgtgc agcacaacgt
     1561 tatcgtaggc tctatcagtg tggtggttat gggcaaacca ttcggaagaa ttaatgtgca
     1621 tctcaacatt tcctacccag gttgttccgt tcaacctaac ctttgcatta aaaaaatcgg
     1681 gaccggcatc agaattatat gtaccggggt ctaacacctc aagctggcag ttgtctgaag
     1741 tatttaagcc cgaaaaggca aatgatttat actgccataa gaaatgaata aaagactcaa
     1801 ccatagtatc aagctgcttc ttatggca
//
LOCUS       DAOOQX010000439         1821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_81639_length_1821_cov_3.459796, whole genome shotgun sequence.
ACCESSION   DAOOQX010000439 DAOOQX010000000
VERSION     DAOOQX010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81639_length_1821_cov_3.459796"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            540..1448
                     /locus_tag="PLX53_07210"
     CDS             540..1448
                     /locus_tag="PLX53_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOY72907.1"
                     /translation="MSHQNESVKSILLALLANLGIAITKTIAAVVTKSGAMMAESIHS
                     FADCANQGLLFWGLKASRKKPNPEHPLGYGKEIYFWSFIVALILFSMGGLFSVYEGIH
                     KVSAHEGLRNTNVAIIVLVVSMVLESISLYGCLKQIRKVRHGESLWKWYKTSRQSELV
                     VVLGEDIAALLGLTLALVAVILSIATGNPIYDAAGSIGIGILLVIVSCFLAVKVKGLL
                     IGQSVDDETSNKIRKLIESRPEVEKIFNLITIQLGPQVMVAVKAKMKATDNINQLISN
                     INACEEALRQEISTIQWVFFEPDLKD"
BASE COUNT          588 a          343 c          399 g          491 t
ORIGIN      
        1 attagtccta aagttttcat tcactcggga gtaaaatttt acagaacaaa taatgtaatg
       61 ttatccggcc gattaaatta ccgtaacaca ttttaaatgt agctttacca ttatgcttac
      121 aatggccctc gccggttaac cagcgaaaag gttcaacaaa aataagtaac aacaaagcac
      181 atacatactg caacagctct tatgctcaga aactcctgtt gaagcagtca cataatacaa
      241 gtaatacaag tataaatgta tacctgagtt ttagcattaa gtcaaaagaa ttgctctacc
      301 aaaactggca gcatcggaat aaaaacattc aatgcgtggc tattccggag taacctggta
      361 agtaagaaaa ataatccgat atgccgaatt atagggaata taagaactcc tgtaaacttt
      421 tagaacatag atgtataggc aataaatgtt tcagccaaaa agtttatcat tctaaaaagt
      481 ctaaaaaata gctcgggtta ctccattttt tgctaactat gcggcaaaat tcaggcaaga
      541 tgtcccatca aaacgagtcg gttaaatcta ttctgctagc ccttttggcg aacctgggaa
      601 ttgcaattac caaaacgatt gcggcagtag taaccaaatc gggtgcgatg atggcggaat
      661 ccattcactc attcgccgat tgtgccaacc aggggctgct attctggggt ttaaaggcat
      721 ccagaaaaaa acccaatccc gaacatcctt taggatacgg aaaggaaata tacttctggt
      781 cgtttatcgt tgccctgata ctgttcagca tgggaggact cttttcggtt tacgagggca
      841 ttcataaggt tagcgcacac gaagggctaa ggaataccaa tgtggctata atcgttctcg
      901 tggtaagcat ggttttggaa tcaatctccc tatacggttg cttaaagcaa atacgtaagg
      961 ttagacatgg agaatcgttg tggaaatggt ataaaactag ccgccagagc gagctggtgg
     1021 tggtcctggg cgaagatatt gcagcccttc tagggcttac cctggcgctc gttgcggtaa
     1081 tactatctat tgcaactggc aaccccattt atgatgcagc aggtagcatt ggtataggca
     1141 ttttgcttgt gatagtttcc tgctttttgg cagttaaggt aaaaggttta ctgattgggc
     1201 aaagcgtgga tgacgaaacc agcaataaaa tcaggaaact aatcgaatca agaccagagg
     1261 tagaaaagat attcaacctg atcaccattc aacttggacc gcaggtaatg gttgctgtaa
     1321 aagcaaaaat gaaagctaca gataacataa atcagctcat tagcaatatt aatgcatgtg
     1381 aagaagcgct taggcaggaa atctcaacca ttcaatgggt attctttgaa cccgacttaa
     1441 aggattaaca cttgtgctca caaaaaaagg tatgcgttag tttctataac cgggcttggt
     1501 ggggctgaac agccggttaa atggtagtaa acgatgtgcg taaaatcaac agggaacaaa
     1561 ccatccgtgc ctaagaggac atctcataca cttaaaattt ttcacattta tttgtaaatt
     1621 tcaattgggc agtatatttt tgcaaggcaa ttcaacagta gagaagacta ttatttgaaa
     1681 gtcttgcgga agtagctcag ctggtagggc ataatccgac taccggcgga taagggttgc
     1741 cagtttgtag tagatttttg aaaattttgc ggaagtagct cagttggtag agcataacct
     1801 tgccaaggtt agggtcgcga g
//
LOCUS       DAOOQX010000440         1821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_81643_length_1821_cov_3.401472, whole genome shotgun sequence.
ACCESSION   DAOOQX010000440 DAOOQX010000000
VERSION     DAOOQX010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81643_length_1821_cov_3.401472"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1585)
                     /locus_tag="PLX53_07215"
     CDS             complement(26..1585)
                     /locus_tag="PLX53_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HOY72908.1"
                     /translation="MATPASILWVDDEIDLLGSHIIFLEQRGYHVQKAINGVDAIELV
                     RNNVFDIVFLDENMPGLSGLETLTQIKELKPHLPVVMVTKSEEENIMDMAVGSQIADY
                     LIKPVNPNQILLSIKKHVHGKTLVSAKQTSDYGVEFSQLSEQIAAAQSYSDWVELYRR
                     LSAWQLRISDNSEPNLRQMLDMQMAEANQLFSRYVRSSYIGWFKGNQDDKPLLSPNLL
                     RSKVFPLLDSGEKVLLVLIDNLRYDQWKAIEPLLADEWRVESDDVYFSILPTATQYAR
                     NAIFAGLMPLEIQQMFPDIWISDDDDEGKNNYELQLLTSQLQRLGKSYKVHYEKGNTL
                     RAGMPIADNVNLVLNNNLTVLVINFVDMMSHARTDSNMMKELASDEAAYISLVRSWFQ
                     HSDLRNILLEAARFDIKVVITTDHGSIRVQNPIKVVGDRATSTNLRYKLGRSLAFNTK
                     EVFEVRDPETAHLPKPNVSSSFIFAYGSNFFVYPNNYNYFASYYRNTFQHGGISLEEM
                     IIPVVVLTPNR"
     gene            complement(1595..>1821)
                     /locus_tag="PLX53_07220"
     CDS             complement(1595..>1821)
                     /locus_tag="PLX53_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72909.1"
                     /translation="NDFVEVVSVTGFQSADIALSYYKLITRDEKVFADFNRAEFQAFL
                     FSTENFNRFMNDKSIPDYSRFFKENYSVDN"
BASE COUNT          478 a          426 c          422 g          495 t
ORIGIN      
        1 tgcatttctc gattggtaac atgggctacc tgtttggagt gagcactacc accggaataa
       61 tcatctcctc caacgatatt cccccatgct gaaaggtgtt acggtagtag ctggcaaagt
      121 agttgtagtt gttgggatac acaaaaaaat tacttccata ggcaaaaata aaggaggaac
      181 ttacgttagg ctttggcaga tgagcggttt cggggtctcg cacctcaaac acctccttgg
      241 tattaaaggc taaacttctc cccagcttat accggaggtt agttgaagtt gccctatcgc
      301 caaccacctt aatggggttt tgaactctaa ttgaaccatg atcggtggtg attacaactt
      361 taatatcgaa acgggcagct tcgagcagga tattcctcaa atcggagtgc tggaaccatg
      421 atcgaaccag cgagatgtag gccgcctcgt cggaggccag ttctttcatc atgttggagt
      481 cggtgcgtgc atgcgacatc atatcaacaa agttgatcac cagcacggta aggttgttgt
      541 tcagcaccag gttaacatta tccgctatgg gcatgcccgc ccgcagggtg ttaccttttt
      601 cgtagtgaac cttatagctt tttccgagcc gttgcagctg ggaggtgagc agctggagct
      661 catagttgtt tttcccctca tcatcatcgt cggaaatcca aatatcggga aacatttgct
      721 gtatttccag aggcatcaac cccgcaaaaa tggcattccg ggcatactgg gtagcagtgg
      781 gcaggatgct gaagtaaaca tcatccgact ctaccctcca ctcgtcggcc agcaggggtt
      841 caatagcctt ccactggtcg tagcgaaggt tatcaataag aaccaggagg accttttcgc
      901 cggaatctaa taacggaaaa accttgctgc gaaggaggtt gggagataat agtggtttat
      961 cgtcctggtt acccttaaac cagccaatgt agctacttcg cacgtaccga gaaaatagct
     1021 ggttagcttc ggccatctgc atatcaagca tctgccttag gtttggctca ctattgtcag
     1081 aaattcttag ctgccaagcg ctaagcctac ggtagagctc aacccaatcg gagtagcttt
     1141 gagcagcagc aatttgctcg ctcagctggc taaattcaac cccataatcg gaagtttgct
     1201 tagcgctaac cagcgtttta ccatgcacgt gcttcttaat ggaaaggagt atctggttgg
     1261 gattaactgg tttaatcagg tagtcggcaa tttgagagcc aaccgccatg tccattatgt
     1321 tctcctcctc gcttttggta accattacca ccggaaggtg gggttttagc tccttaattt
     1381 gtgtaagtgt ttcgagcccg cttagcccgg gcatattttc atcgaggaaa acaatgtcga
     1441 aaacattgtt acgaaccagc tcaatggcat ccaccccatt gatggccttc tgcacatggt
     1501 aaccccgctg ttcgagaaag attatatgtg aacccaatag gtctatttcg tcatcaaccc
     1561 aaagtataga tgctggtgtt gccatagcat tttgttagtt gtcaacactg tagttttctt
     1621 taaaaaatct ggaatagtct gggatagact tatcgttcat gaaccgatta aaattctcgg
     1681 tgctaaaaag gaaagcttga aattcggcac ggttaaaatc ggcaaacacc ttttcatccc
     1741 gcgtaatcag cttgtagtag cttagggcaa tatcggcact ctgaaacccg gtaacactta
     1801 ccacctcgac aaagtcgttt a
//
LOCUS       DAOOQX010000441         1821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_81644_length_1821_cov_3.378822, whole genome shotgun sequence.
ACCESSION   DAOOQX010000441 DAOOQX010000000
VERSION     DAOOQX010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81644_length_1821_cov_3.378822"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PLX53_07225"
     CDS             complement(<1..653)
                     /locus_tag="PLX53_07225"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOY72910.1"
                     /translation="MTKIKYFAVTLVLLLGMGSCSQDFLEMEPLTDRVEGNFYKTEKD
                     AFEALVSIYDVLQWQSSYGYHPWDLVSNVLSDDAFSGGSGPGDRPGLNRMGKFSSYTT
                     DEEALGLWKDRYSGIYRANIFLEKIDGIQFKDEALKDRYIAEARFLRGYFYFELVQFY
                     GHVPLILKTLTTSEYKQSQANPADVYNQIASDLLFAWEKLPGNISAAEYGRVSKWAAG
                     "
     gene            complement(673..>1821)
                     /locus_tag="PLX53_07230"
     CDS             complement(673..>1821)
                     /locus_tag="PLX53_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY72911.1"
                     /translation="IGAFKYTSIIGSGSKYTFGSDEVITVGSNPSGIPNPDLKWETSE
                     QYNIGIDSRFFNERLNFTLDLYNKKTKDLLVVAPIPGYIGNAAPTVNGGTVTNKGVEL
                     ELGYKTSINKLDVDVSLSGAYNKNEVTDINNAEGKIYGAGLAVGMSNICMMQEGFPIA
                     YFWGYKTAGVFQNEAQILSYISSDSTVIQPNAKPGDLIWVDKNDDGKIDDNDRMLIGN
                     PYPDFTAGLNVNLAYRNFDFNMFWYGAFGQDIFDGTRRYDLPMSNWNASVLDRWTGEG
                     TSNSNPRVTIADPNKNYVNVSDFYVHNGSYLRLKNLTLGYTIPTTISQKVKIQRMRVF
                     MSVSNLLTFTSYKGFDPEIGAKSALDVGIDRNIYPQSRTFMFGVNLNF"
BASE COUNT          532 a          397 c          368 g          524 t
ORIGIN      
        1 ccggcagccc attttgaaac cctaccatac tcagcagctg aaatgttgcc tggcaatttt
       61 tcccatgcaa agagcagatc gcttgcaatt tgattgtaca catcagcagg attggcctga
      121 gattgcttat actcagatgt ggtaagggtt ttaaggatta agggaacatg accgtagaac
      181 tgaaccagct caaagtaaaa gtatccccta agaaaacgtg cttcggcaat gtagcgatct
      241 ttaagtgctt catctttgaa ttggattcca tcaatttttt cgagaaaaat attggctctg
      301 tatataccgg agtacctgtc tttccataaa ccaagcgcct cctcatcggt ggtatagcta
      361 gaaaactttc ccattctatt aagacctggt ctatcgccag ggccagatcc accactaaaa
      421 gcatcatcgg aaagaacgtt agaaactaaa tcccaaggat gataaccgta gctcgactgc
      481 cactggagaa catcatatat agaaaccaat gcctcaaagg catccttctc tgttttgtaa
      541 aagttaccct caaccctgtc ggttaaaggc tccatctcca aaaagtcctg agagcacgaa
      601 cccattccga gaagtaacac tagggtaaca gcaaagtatt taatttttgt catggtgata
      661 aaattttgtg gtttagaaat tcaggttgac tccgaacatg aaggtccgtg attgagggta
      721 aatgttgcgg tcaataccaa catcaagtgc agattttgca ccaatctcag ggtcaaaacc
      781 tttatagcta gtaaaggtta aaaggttgga aaccgacata aaaactctca tccgctgaat
      841 tttcaccttt tggctaatag tagtaggtat agtatagccc agggtaaggt tcttaagtcg
      901 aaggtaagag ccattatgaa cataaaagtc ggaaacattt acatagtttt tgttgggatc
      961 ggcaatggta acccttggat ttgaattaga ggttccttcg cctgtccaac ggtcgagaac
     1021 actagcattc cagtttgaca tgggcagatc gtaccggcgg gttccatcga atatatcctg
     1081 gccaaaagca ccgtaccaaa acatgttaaa atcgaagttc cggtatgcca ggttaacatt
     1141 taacccggcg gtaaaatcgg gatatggatt tccaatcaac atacggtcat tatcgtcaat
     1201 tttaccgtca tcatttttat caacccaaat aaggtcgcca ggtttagcat taggttgaat
     1261 aacggtgcta tcggatgaaa tgtagctaag aatttgagct tcattctgaa agaccccggc
     1321 agttttataa ccccaaaagt aagctattgg aaatccctcc tgcatcatgc agatattact
     1381 cattccaacg gcaagtcctg ctccataaat tttaccctcg gcattattta tatcggttac
     1441 ctcattttta ttgtaggcgc cgctaaggct tacgtctaca tccagcttgt tgatgctggt
     1501 tttataaccc agctctagct caacaccctt gttggtaact gttccaccat ttacggttgg
     1561 tgctgcatta ccaatatacc ctggaatggg agcaactacg agtaagtctt ttgttttctt
     1621 gttatacaag tctagagtaa agtttaacct ctcgttaaaa aaccgcgagt cgatacctat
     1681 gttgtactgc tcggaggttt cccattttaa atcgggattg ggtatgcccg aaggattgga
     1741 acctaccgtt attacctcat ctgaaccaaa tgtgtacttg cttcctgaac caattattga
     1801 ggtgtacttg aatgctccaa t
//
LOCUS       DAOOQX010000442         1820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_81710_length_1820_cov_3.518980, whole genome shotgun sequence.
ACCESSION   DAOOQX010000442 DAOOQX010000000
VERSION     DAOOQX010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81710_length_1820_cov_3.518980"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLX53_07235"
     CDS             <1..540
                     /locus_tag="PLX53_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOY72912.1"
                     /translation="YGIVSDSKDATYEVLAKAIAENEVVTLTGGVSMGDYDYVPEIME
                     KLGVTIHFSSVAVQPGKPTTFGTLDSKLVFGLPGNPVSAFIQFELLVKPAIRKLMGCK
                     QLNPTAIRLPLAEDYRRNRAERMALVPVKIIAGSAALPVEFHGSAHIFALGNADGIIA
                     VPIGTTKIDKGEIVDVRPL"
     gene            503..1324
                     /locus_tag="PLX53_07240"
     CDS             503..1324
                     /locus_tag="PLX53_07240"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY72913.1"
                     /translation="MIREKLLMYDRFNRQINYLRISVTDRCNLRCTYCMPEEGITQLD
                     HSEILSFEEIASFTRIAATKGFNKVRITGGEPLVRKGVVELVSMISNIEGITDLSMTT
                     NGVLLGKYAKELKYAGLQRVNVSLDTVNPTRYRQITRVGSLDDVLNGLECARAAGLTP
                     IKINCVVDKSSAEPDAVEVKKFAQANGFEVRFIPKMDLNAGIFGEVEGGNGGNCSICN
                     RLRLTANGFVKPCLFSNLGYSVRGLGYEGALLKAVNAKPARGTTNHDGKFYNIGG"
     gene            1326..1781
                     /gene="moaC"
                     /locus_tag="PLX53_07245"
     CDS             1326..1781
                     /gene="moaC"
                     /locus_tag="PLX53_07245"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOY72914.1"
                     /translation="MELSHTDRDGRANMVDVGSKPNQLREARAKGFIRLQPETLKLIT
                     ENQVKKGDVLTLAEIAGIQAAKQTPTLIPLCHTILLAKVDVKASIVQNGIEISSYIKC
                     IGQTGVEMEALTAVSVALLAVYDMCKAVDKEMTIESIKLTEKIKHDINS"
BASE COUNT          534 a          413 c          455 g          418 t
ORIGIN      
        1 tatggcattg tgagtgatag caaggatgct acctacgaag ttctagccaa agccattgcc
       61 gaaaacgagg ttgttacgct aacaggtggc gtttcaatgg gcgattacga ctacgtgccc
      121 gaaatcatgg agaagttggg ggttaccatc cacttcagca gcgtagcagt tcagcccggc
      181 aagcctacaa cgttcggaac cttggacagc aagctggtat ttgggctgcc aggtaatcct
      241 gtttcggcat tcatacagtt cgagctactg gttaagcccg ccatacgtaa gctaatgggc
      301 tgcaagcagc tgaaccctac ggccattcgt ttgcctttag cagaggatta caggcgaaac
      361 agggccgagc gaatggcgct ggttccggtt aagataatag cagggtcagc agctttacct
      421 gttgagtttc acggctcggc acacatattt gcccttggca atgccgatgg cattattgct
      481 gtccctattg gtacaacaaa aattgataag ggagaaattg ttgatgtacg accgctttaa
      541 ccgccaaatc aactacctgc ggatatcggt aaccgaccgt tgtaacctac ggtgcactta
      601 ctgcatgccc gaagagggta ttactcagct tgaccatagc gagatactct cgtttgagga
      661 aattgcatcc tttacaagaa ttgcagccac aaaagggttc aacaaggtga gaattacagg
      721 cggtgaaccc cttgttcgaa aaggtgtagt ggaactcgtg tccatgattt ccaacattga
      781 gggcatcacc gacctgagca tgaccaccaa cggagtgctt ctgggcaaat atgctaagga
      841 gttaaaatat gctggtttac agagagttaa tgttagcctc gatacggtca accccaccag
      901 gtacaggcaa attaccaggg taggtagcct tgacgatgtg cttaatgggt tggaatgtgc
      961 cagagctgca ggcctaaccc caataaaaat aaactgtgta gttgataaat catcggcaga
     1021 acccgacgcc gttgaggtaa aaaaatttgc ccaagcaaat ggatttgaag tcaggttcat
     1081 tcctaaaatg gatttaaacg ccggaatatt cggcgaggta gaggggggaa acggcggtaa
     1141 ctgcagcatc tgcaatcgcc tcaggttaac agccaacggt tttgtcaaac catgtctgtt
     1201 tagtaatctg ggctatagtg tacgggggct tggatatgag ggagccttac tcaaagcagt
     1261 gaatgccaaa cctgcccgtg gaactaccaa ccatgacggg aaattctata acataggagg
     1321 ttgacatgga actttcacat accgacagag atggcagggc caacatggtt gacgttggct
     1381 ccaagcccaa ccagcttagg gaagcacgcg ccaaaggatt tatcaggctt caacccgaaa
     1441 ccctaaagct tatcacagag aatcaggtaa aaaagggtga tgtgctcacc cttgccgaga
     1501 ttgccggaat acaggctgcc aaacaaacgc caacgctcat cccgctttgc cacaccatac
     1561 tgctagctaa ggttgatgta aaagcatcca tcgttcaaaa cgggattgaa ataagcagct
     1621 acattaagtg catcggccaa acaggtgttg aaatggaagc cctcacagcc gtttcagttg
     1681 ccctgttggc tgtttacgac atgtgcaaag ccgttgacaa ggaaatgaca attgagtcca
     1741 taaaactcac cgaaaaaata aaacatgaca ttaattcgta aagcaaaaag ttgtggatac
     1801 cagcgtaaaa gtactatcgg
//
LOCUS       DAOOQX010000443         1818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_81836_length_1818_cov_4.635848, whole genome shotgun sequence.
ACCESSION   DAOOQX010000443 DAOOQX010000000
VERSION     DAOOQX010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81836_length_1818_cov_4.635848"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="PLX53_07250"
     CDS             <1..1201
                     /locus_tag="PLX53_07250"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOY72915.1"
                     /translation="GLEYYQLYIAARDSVMGTRNLRKMLNVELQDEYSRLIKEHFERK
                     DQEILSKEQAQQSKTVLLYILSGAFIILLLFLVMFIRTLKNKQKINNMLIAQQAEVEH
                     QKQELMNQRDEIDTQKEMVVYQRDRIITMLTDLAESIDYAKKIQQAILPKDSLLQQLF
                     TDYFIIYQPKEAVGGDFYWAGRFNGTICFAVADCTGHGVPGGFMSMLGVSMLNDMATA
                     DSVQSPSLMLQSLRHNIVAALGQTNFDEDNHDGMDIAFCTFDIKSGILTYAGANMPII
                     INSKGEIPPSERIQPYQNGLYELKPDRMPVALYDKMDSFSEIRVKLSPGDAVYLFTDG
                     YVDQFGGPKSKKFGHDAFRTLINSVKDLSFSEQKQIIWSTIERWKGETENQTDDILVM
                     GLKLPDK"
     gene            1232..1807
                     /locus_tag="PLX53_07255"
     CDS             1232..1807
                     /locus_tag="PLX53_07255"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOY72916.1"
                     /translation="MKHELPQLPYSADALSPVISAKTIEFHYGKHHQAYVNNLNNLIV
                     GTPFENATLETIIKEAEGGIFNNGAQVWNHTFYFLTLSPNPKSEPSGKLGEQISLDFG
                     SFAEFKDKFEKASAGLFGSGWTWLVLNSKGQLEITQESNAGNPLRKGLKPLLACDVWE
                     HAYYLDYQNRRPDYLHSFWNIVDWKVVEGRF"
BASE COUNT          552 a          415 c          400 g          451 t
ORIGIN      
        1 aggactggaa tactaccagc tatacattgc cgcccgcgat agtgtgatgg gtacacgaaa
       61 cttaaggaaa atgcttaatg ttgagctgca agatgagtac tcccgactta ttaaagaaca
      121 ctttgaacga aaagatcagg agattttgtc taaagaacag gcacaacaaa gcaaaacagt
      181 tttactctat atactttcgg gggcatttat cattcttttg ctattcctgg taatgttcat
      241 ccgcacctta aaaaataaac aaaaaataaa taacatgctt atagcccagc aggcggaggt
      301 ggagcatcaa aaacaggagc tgatgaacca gcgcgacgag attgacaccc agaaagaaat
      361 ggtggtttac cagcgtgata ggattattac catgctcacc gatttggcag aatcaattga
      421 ctatgcaaaa aaaattcagc aagccattct accaaaagat tccctcctgc agcagctatt
      481 taccgattac ttcataatat accagcctaa ggaggctgtt ggaggcgatt tctactgggc
      541 cggccggttc aacggcacca tttgctttgc agtagccgat tgtaccggac atggcgtccc
      601 tggaggcttt atgagcatgc tgggagtttc aatgcttaac gatatggcta ctgccgacag
      661 cgttcaatcc ccatcattaa tgctgcaaag cctcaggcac aatattgtgg ctgcccttgg
      721 gcaaacaaac tttgatgagg ataaccatga tgggatggac attgccttct gcaccttcga
      781 catcaagtcg ggtattttaa cttacgcggg agccaacatg cctatcatta ttaactctaa
      841 aggcgaaata ccaccctccg aacgtattca gccctatcaa aatgggctct atgaactaaa
      901 accagaccga atgccagttg cactttacga taagatggac tcctttagcg aaattcgcgt
      961 caagctatcg ccaggagatg ccgtttacct ttttaccgat ggatatgttg accagtttgg
     1021 tgggccaaaa agtaagaagt ttggacatga cgccttccgt acacttatta actcagtgaa
     1081 agatttaagc ttttccgagc agaagcaaat catttggagt accatcgagc gctggaaggg
     1141 agaaaccgag aaccaaaccg atgacatcct ggttatggga ctaaagctcc ccgacaaata
     1201 aaaaacagtg taacctttaa aattaaccaa gatgaaacat gaactgccgc agctaccgta
     1261 ctcagccgat gccttgagcc cggtaatttc tgccaaaacg attgagttcc attacggaaa
     1321 gcaccaccag gcttacgtaa acaatctcaa caaccttatt gttgggacac cgtttgagaa
     1381 cgccacactt gagactatca tcaaggaggc agagggtgga attttcaaca acggtgcaca
     1441 agtttggaat cacaccttct acttcctcac tctttcgccc aatccaaaaa gcgaaccgtc
     1501 aggaaaactc ggagagcaga tttctttaga cttcggctca tttgcagaat ttaaagataa
     1561 atttgaaaag gcttcagccg gcctgtttgg ttccggatgg acctggttgg ttttaaactc
     1621 aaagggccag ctggagatta cccaggagtc aaacgcggga aatccccttc gcaaaggcct
     1681 aaagcctctt ttggcgtgtg atgtatggga acacgcctac tacctcgatt accagaatcg
     1741 ccggcccgat taccttcata gcttctggaa tatagttgat tggaaagttg tagagggaag
     1801 gttttagcta aaagattt
//
LOCUS       DAOOQX010000444         1817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_81949_length_1817_cov_2.786039, whole genome shotgun sequence.
ACCESSION   DAOOQX010000444 DAOOQX010000000
VERSION     DAOOQX010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81949_length_1817_cov_2.786039"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1806
                     /locus_tag="PLX53_07260"
     CDS             <1..1806
                     /locus_tag="PLX53_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOY72917.1"
                     /translation="IFGGVLADKVLGYGKTILLGILVMFIGYLLLAIPLEGNSVGLTM
                     MIISLVLIALGTGFFKGNLQALVGKLYDDKRYDTNRDIAFSIFYMFINIGAFFAPSAA
                     NYINNAVMKSSDFTYDASIPKNYVVLNDEKQAVDKNTFIVQNLTPAEQQLSGKEFDAV
                     LTKAKKKHKVVFTEDIEHALFRLKAAGLNQYTQSGKITDPAMMISNNEYELLQSRSQE
                     DMLKKAEVTAKIQAISITDPALEGGPNPDDFARNFGKHYVSESLSTSYNLAFGVACIS
                     LIISVLIFLLFKKSWKSVDKTVKQEIAESKANGKEVKVMSKEEVRERTVALLLVFFVV
                     IFFWMSFHQNGLTLTFFARDYTQSVISPGWTMLFNLFPLLSLIVFLYGIYLSTIGLLG
                     GKPNKKSGLLLTVLGIAGLAGYYFTRVKGIEVMTKITPQIFQQFNPMFIVILTPLTVA
                     LFSYLGKKKKEPSAPRKIGIGMFLAAIGFTIMLICSFGLPAPSEISGVSDQLVSPAWL
                     ISTYFVLTVAELFLSPMGLSFVSKVAPPQYSGLMQGGWLAATAIGNLLVGVIGTFWEK
                     LPLVGFWVILIICCVISGIFIFSVMKRLEKATGNV"
BASE COUNT          451 a          409 c          432 g          525 t
ORIGIN      
        1 atttttggtg gagttctggc ggataaggtg ctgggctacg ggaagaccat tttgctgggt
       61 attttggtca tgtttatagg gtatttactt ttggctattc cactcgaagg caattctgtg
      121 gggctgacta tgatgattat ctcgctcgtt ttgattgcgc tgggtaccgg attcttcaaa
      181 ggaaatttac aggcgctggt aggtaaactt tacgacgaca agcgttacga caccaaccgg
      241 gatattgcct tttccatttt ttacatgttc atcaatattg gcgccttttt tgccccaagt
      301 gctgctaact acatcaacaa tgccgtgatg aaatcgtccg atttcaccta tgatgcctca
      361 attcctaaaa attatgttgt tctgaacgat gagaagcaag cagtagataa aaacaccttt
      421 attgtacaaa accttacacc ggccgagcag cagctctccg gtaaggagtt tgatgcagtg
      481 cttactaaag ccaaaaagaa gcacaaggtg gttttcaccg aggatattga gcatgcgctt
      541 ttccgtctga aggctgctgg gctgaaccag tacacccagt cgggcaaaat taccgatccg
      601 gctatgatga ttagcaacaa cgagtatgag ctgctgcaaa gccgtagcca ggaggatatg
      661 ctgaaaaaag ctgaggttac tgccaaaatt caggcaatca gcattaccga tccggcgctg
      721 gaaggaggtc ccaatcccga cgattttgcc cgtaactttg gtaaacacta cgttagcgaa
      781 tcgctatcta catcctacaa cctggccttt ggcgtggcct gcatcagcct gattatctct
      841 gtactcatat tcctgctttt caaaaaatcg tggaaatcgg ttgataaaac agttaagcag
      901 gaaatagccg aatcaaaggc caacggcaag gaggttaagg tgatgtcgaa ggaggaggtt
      961 cgggaacgaa ccgtggcgtt gctgcttgta tttttcgtag taatcttttt ctggatgtcg
     1021 ttccatcaaa acgggttaac gctaaccttc tttgcgcgcg actataccca gagcgtaatc
     1081 tcgcccggtt ggactatgct gtttaacctg ttcccattgc tatcgcttat tgttttcctt
     1141 tatggaattt acctttctac aatcggattg ctgggtggta aacccaataa aaaatcagga
     1201 cttttactaa cggtgttggg tattgccgga ttggcaggct actactttac ccgcgttaag
     1261 ggaattgagg taatgactaa gattaccccg cagattttcc agcagtttaa ccccatgttc
     1321 atcgtcatcc taaccccgtt aaccgttgcg ctgttcagct atttaggtaa gaagaaaaaa
     1381 gaaccttcgg ctccccggaa aattggtatc ggtatgtttc tggcagccat tggttttacc
     1441 attatgctaa tctgctcttt cggactccct gcaccctccg aaatttcggg tgtttccgat
     1501 cagctggtgt ctccagcctg gctcataagc acctatttcg tgttaactgt agccgagcta
     1561 ttcctcagcc ccatggggtt atcctttgta tcgaaagttg caccgccaca gtacagcggt
     1621 cttatgcagg gtggatggct tgcagctacc gctatcggca atttgctggt aggggtaatc
     1681 ggcactttct gggaaaaact tccactggtt ggcttctggg taattctcat tatttgctgc
     1741 gtaatttcgg gcatattcat cttctccgtc atgaagaggc tcgaaaaagc cacagggaat
     1801 gtatagggga ggttgaa
//
LOCUS       DAOOQX010000445         1813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_82247_length_1813_cov_3.435154, whole genome shotgun sequence.
ACCESSION   DAOOQX010000445 DAOOQX010000000
VERSION     DAOOQX010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82247_length_1813_cov_3.435154"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1756)
                     /locus_tag="PLX53_07265"
     CDS             complement(<1..1756)
                     /locus_tag="PLX53_07265"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOY72918.1"
                     /translation="MRKLSGFLSFFFIILLVLPASKIAAQNIKISGTVISLTGGYPIR
                     GVTVVVVGSARGTFTDSLGRYELVTPSISKTLRFTCIGYKTQEVDIAGRRVIDVALEE
                     EAIEMQEVKVTALGIMRAERSTGYALQSVKGEDINRTSETSLLNALQGKVAGAIITST
                     SGAVGASSRIVLRGINSLNTDNQPLFVVDNVVLSNANFGNTTTEGVNRGSGVADINPN
                     DIEDITVLKGPNAAALYGSRAANGVIIIKTKSADVDDHFGVSFNSTITMESPMRLPEF
                     QNKYGQGSGGLFEFYDGMGNGINDNVDESWGPPLDIGLMIPQWNSPVAGGIRQPTPWI
                     SHPDNVKNFFETGMTFTNNVSVEGADESYAARFSYTNTEQKGMVPNTDQSKNNVMLNA
                     SISPYTYLTLNASLGYVNTHSDNLPAYGYDAQNVMQQFLWFGRQVDVADLRQYRFPDG
                     SKRNWNYSYHNNPYFTLYENLNGLDRDRLFGQGSINLKFTDWLNLKVGAGIDYFKNYN
                     NARSAFGDIDSPLGYYGESHLTFRELNTDFLLSFNRNLSSKLSLSFNLGGNLMRQYSQ
                     SLNSYATELSVEDVYTLEN"
BASE COUNT          447 a          426 c          414 g          526 t
ORIGIN      
        1 tattctcaag cgtgtaaaca tcttcaaccg atagctcggt ggcgtaggaa ttgagcgatt
       61 gggagtattg cctcatcagg ttacctccaa ggttaaagct taacgatagc ttgcttgaaa
      121 ggttgcggtt gaaggagagc agaaagtcgg tattcaactc cctgaaggtc agatgtgatt
      181 cgccatagta gcccaacggg ctgtctatat ctccaaaagc ggagcgggcg ttgttgtagt
      241 ttttaaagta gtcaatgcct gcaccaactt taaggttgag ccaatcggta aatttgaggt
      301 ttatacttcc ctgtccaaac agcctgtcgc ggtcaagccc gttgaggttt tcatacaggg
      361 tgaagtaggg gttattatgg tagctgtagt tccaatttcg ctttgacccg tcgggaaatc
      421 tatactgcct caaatcggca acatcaacct ggcggccaaa ccaaagaaat tgctgcataa
      481 cgttttgtgc atcgtagccg taggcgggca ggttgtcgct gtgggtgttc acgtagccaa
      541 ggctggcatt tagggttagg taggtatagg gcgaaatgga ggcgttgagc attacgttat
      601 tcttcgactg gtcggtgttc gggaccattc ctttttgctc ggtattagtg taggagaagc
      661 gtgcagcata gctttcatca gcaccttcaa ctgaaacatt gttggtaaaa gtcattcctg
      721 tttcaaaaaa attctttaca ttgtcgggat gggaaatcca aggagtaggt tgcctaatcc
      781 cgcctgccac aggagagttc cattgaggta tcattagccc aatgtcgaga ggcggtcccc
      841 agctttcatc aacattgtcg ttaatgccat tacccatgcc atcataaaat tcaaacaaac
      901 caccagagcc ttgtccgtac ttattttgaa attctggcag ccgcattggc gactccatgg
      961 tgatggtgct gttgaacgat acgccaaaat gatcgtcaac atcggcactc ttggtcttaa
     1021 tgattataac cccattggct gcacgcgatc cgtagagtgc tgccgcatta ggccctttta
     1081 gaacggtaat atcctcaata tcattaggat taatatcggc aacgccactt cccctgttca
     1141 ccccttcggt tgtagtgttt ccgaaattgg cgttgctcag caccacgtta tcaaccacaa
     1201 atagaggctg gttatcggta ttgagcgagt taattcccct aagcactatt ctggatgatg
     1261 cgcctactgc cccggacgtg cttgtaataa ttgcgcctgc tactttcccc tgaagagcat
     1321 tcagcaggct tgtctcgctg gtgcggttaa tgtcttcacc ctttacgctt tgtaaagcat
     1381 agccggttga tcgctccgcc cgcattattc ccaacgctgt aacttttacc tcctgcattt
     1441 caatggcctc ttcctcaagc gcaacgtcta taacccttcg tccggctatg tcaacctcct
     1501 gggtcttata gccaatgcat gtaaagcgta gagttttgga gattgatggg gttaccagct
     1561 cgtaccggcc caggctatcg gtaaatgttc cccgtgccga tccaactact accactgtaa
     1621 ccccccttat gggatatcct cctgttagtg aaattactgt tccacttatt tttatgttct
     1681 gtgctgctat tttgcttgcg ggtaatacca gtaaaataat aaaaaaaaat gagaggaagc
     1741 cactcaattt tctcatagaa acaaccattt taaatatttc tgattcgaac ctgcttaaat
     1801 attaagctat ttg
//
LOCUS       DAOOQX010000446         1809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_82564_length_1809_cov_2.396237, whole genome shotgun sequence.
ACCESSION   DAOOQX010000446 DAOOQX010000000
VERSION     DAOOQX010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82564_length_1809_cov_2.396237"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..236)
                     /locus_tag="PLX53_07270"
     tRNA            complement(150..236)
                     /locus_tag="PLX53_07270"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(200..202),aa:Leu,seq:gag)
     gene            complement(352..435)
                     /locus_tag="PLX53_07275"
     tRNA            complement(352..435)
                     /locus_tag="PLX53_07275"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(399..401),aa:Leu,seq:cag)
     gene            complement(507..>1809)
                     /locus_tag="PLX53_07280"
     CDS             complement(507..>1809)
                     /locus_tag="PLX53_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOY72919.1"
                     /translation="VRLNRLQNKVDILLADASTGSSSVLYTETNKYYIEEVTDAYPTF
                     TPDGKYFIITSEKDGYRHIYLYGMNGSLVNQLTAGINEVTEMYGYSPLSKMVYYQAYD
                     DSPLCKAVYAKKIDGKQPVKLSTNIGNNYAQFSSNFEYCMIFHSNIQEPLTVSLINAK
                     GKEVRVVEDNQKLRDRLKEFAIPSKEFYTFTTSEGVELNGYMVKPVDFDSTRKYPVMM
                     YQYSGPGSQTVTNSFRVGWDEYLATLGYLVVCVDGRGTGGRGEAFRKMTYGQMGYYES
                     IDQIEAAKYLQTLSWVDAGRIGIWGWSYGGYMSSLCLFKGADIFKMAIAVAPVSNWRY
                     YDSIYTERFMGLPQDNPLGYDSNSPINHADKLRGKLLLVHGTADDNVHFQNSMELVER
                     LVQSGKQFDMMIYPDKNHGIYGGNTTMHLYTLLSNYVQENL"
BASE COUNT          483 a          423 c          399 g          504 t
ORIGIN      
        1 cttgccgttg ggagtggtga acgagtcggc gctttgggca aacgagccgg tgaaagccga
       61 aaccagcaaa agggtaagtt ttacttttct catggtatta gatgttgatt tacccaaaaa
      121 taaaaaaacc ttgcaaaaat tacaaggttt ggtgcccagg acaagactcg aacttgcacg
      181 cttttgacgg cgccagcccc tcaagctggt gtgtctacca attccaccac ctgggcaata
      241 acaactggga attaacgaat gcaaaaatag cctatttttt caaatttcta ccgcaccagg
      301 gcgatatttt tggcgttttc gtaataaagg ttcaccatgg ggtgaacctt gtgcccagaa
      361 caggactcga acctgcacat cattgctgat actagtccct gaaactagcg cgtctaccaa
      421 ttccgccatc tgggctaaaa tgcggatgca aaattataca atttctgatt catccaaaca
      481 cgacacccgg aaatttagca ggaacactat aaattttctt gtacgtagtt gctcaagagc
      541 gtgtagaggt gcatggtggt gttacccccg tatatgccat gatttttatc aggatatatc
      601 atcatatcga actgttttcc actttgcaca agtcgttcga ctagctccat gctgttctga
      661 aagtgaacgt tgtcgtcggc agtgccatgc accagcaaaa gtttccctcg aagcttatcg
      721 gcatggttaa ttggtgagtt ggaatcgtag cccagaggat tgtcctgggg tagccccata
      781 aatcgttcgg tatagatgga atcgtagtaa cgccagttgg aaaccggagc aacagcaatg
      841 gccattttaa aaatatcggc tcccttgaaa aggcaaaggc tcgacatata tccaccgtag
      901 ctccaacccc aaattccaat tcttcctgca tcaacccatg aaagggtttg caggtacttt
      961 gcagcctcaa tctggtcgat ggattcgtag taacccattt gcccgtaagt catcttccta
     1021 aacgcttctc cccttccacc ggttcctcgt ccatctacgc aaacaacaag gtatccaaga
     1081 gtggccaggt attcgtccca accaactcta aagctgttgg ttactgtttg agatccggga
     1141 ccgctgtact ggtacatcat tacagggtac ttccgggtgg agtcaaaatc aaccggttta
     1201 accatgtaac cgttgagctc aaccccctcg ctggttgtga atgtataaaa ttcctttgag
     1261 ggaatggcaa actccttaag cctatccctt aacttctggt tatcctctac caccctaacc
     1321 tccttgcctt tggcgttaat cagtgaaaca gtaagcggtt cttgtatgtt ggagtggaaa
     1381 atcatgcagt actcgaaatt gctgctgaac tgagcatagt tgttgccaat atttgttgac
     1441 agctttacag gttgtttacc gtcaattttc ttagcataaa ctgccttaca aagaggcgaa
     1501 tcgtcgtagg cctggtagta aaccattttt gataaagggc tgtagccgta catctctgtt
     1561 acctcgttaa tgcccgcagt aagctggttt acaaggctgc cgttcatgcc gtaaagataa
     1621 atgtgtcggt aaccatcctt ttcggaggta ataataaaat acttaccatc gggggtgaag
     1681 gtcgggtagg cgtcggttac ctcttcaatg tagtacttgt ttgtttcggt ataaagcaca
     1741 cttgaactgc cggttgatgc atcggccaaa agaatatcta ctttattttg aagacggttg
     1801 agcctaacc
//
LOCUS       DAOOQX010000447         1805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_82860_length_1805_cov_2.413143, whole genome shotgun sequence.
ACCESSION   DAOOQX010000447 DAOOQX010000000
VERSION     DAOOQX010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82860_length_1805_cov_2.413143"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..724
                     /locus_tag="PLX53_07285"
     CDS             35..724
                     /locus_tag="PLX53_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOY72920.1"
                     /translation="MHPNINKNEFKWLRRISMKFINRRDFIRIERSSVSLAFKKGETI
                     LKQGEQPSHVVYLERGIVKFSYQNEAGKNLILTIVAAPKILGGANLFYKDNNLFSFVA
                     VDDCDVVLIDAQVLLSVMENNAKFSIMLFQLASGMFKKSILNFISLAYKQKEGRIADI
                     IIYLSAEVYNSRDFTLTLTRRELAEFAGCSTENVIMTLSKWQNENVIAAEGKQLQIID
                     MERLKHISKIA"
     gene            727..1134
                     /locus_tag="PLX53_07290"
     CDS             727..1134
                     /locus_tag="PLX53_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOY72921.1"
                     /translation="MLTQKTRYSMLAMIRLAREYGKGTLLINQIAESERIPKRFLEAI
                     LLELKNNGYLGSKLGKNGGYYLLKKPEEISLLEIIRLFEGSISLLQCTSERFYQPCEH
                     CKDENSCPLRTVFGDIRSYTFNRLDETTLDKLL"
     gene            1406..>1805
                     /locus_tag="PLX53_07295"
     CDS             1406..>1805
                     /locus_tag="PLX53_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HOY72922.1"
                     /translation="MAIALGISASNPQNVKGHPEEYVLLISFDGFRWDYPTMYHTPNL
                     DLIGIEGVHAKSLISCYPSKTFPNHYSLATGLYPDHHGIVNNSFFDKKLGYYSIGNRK
                     SVENGEFYQGEPIWVTAEKQGIRTASYYWVG"
BASE COUNT          564 a          393 c          391 g          457 t
ORIGIN      
        1 atatttaata ctttttccaa acagcctata actaatgcac cctaatatca acaaaaatga
       61 gttcaaatgg ttgcgaagga tttcgatgaa gtttatcaac cgcagggatt tcatcagaat
      121 tgaaaggtcg tcggttagct tagccttcaa aaagggagaa accatcctga aacagggaga
      181 acagccatcc cacgtagttt acctcgaaag gggcatagtg aagtttagct accagaatga
      241 ggcaggcaaa aacctgatac ttaccattgt tgcagcacca aaaatacttg gtggcgctaa
      301 cctattctac aaggacaata acctcttttc atttgttgcc gttgatgatt gcgatgtggt
      361 gctcatagac gctcaggttc tcctgtcggt aatggaaaac aacgctaagt tctccattat
      421 gctgtttcag ctggcctcag gcatgtttaa aaagtccata ctgaacttca ttagcctggc
      481 gtacaagcaa aaggaggggc gaatagccga tatcatcata tacctctcag ccgaggttta
      541 caactcgaga gattttaccc tcaccctgac ccggagggag ctggcagaat ttgccggatg
      601 ctcaacggaa aatgtcatta tgaccctctc gaaatggcaa aacgaaaatg tgatagcagc
      661 tgagggtaag caattacaga taattgatat ggaacgacta aagcacatca gcaaaattgc
      721 ctagccatgt tgacccaaaa aacaagatac tcaatgctgg ccatgataag gttggccagg
      781 gaatacggca aaggaaccct gttgataaat caaattgccg aaagcgagag aatacccaaa
      841 cgttttcttg aagctattct gctggagctt aaaaataatg gctaccttgg aagcaagttg
      901 ggtaaaaacg gcgggtacta cctgcttaag aaaccagaag aaataagcct gctggaaatc
      961 ataaggcttt ttgagggaag catctcgctc cttcagtgca cttccgaaag gttttaccag
     1021 ccatgcgagc attgcaagga cgaaaactcc tgcccgctgc gcacagtttt tggcgacata
     1081 agaagctaca catttaacag gttggacgaa actaccctcg ataaactttt atgaagaact
     1141 ttttctcctg tgcataaaac caaaactctt agaccgtgtc gaactatatc ggagttaacc
     1201 caccgtatta cacgttagaa atcatacagg ttaactttac ggcaagaggg aaactttcag
     1261 ggaaacaatg ccaatactgt tgaacaaatt atgatttatg tgtttgtact acgagtttca
     1321 atttcaggca aagccatcca ttaccttata aggttgtaaa gaaatgaaga aaaaattaaa
     1381 agttattttg ctgacagtaa gctgtatggc tattgcttta ggcattagcg catcaaatcc
     1441 gcagaacgtg aagggtcatc ctgaggagta tgttctgctt atctcatttg acggttttcg
     1501 gtgggactac cccaccatgt accatactcc caatctcgac ctgatcggaa ttgagggtgt
     1561 tcacgccaaa tcgttaatta gctgctaccc ctccaaaact ttccccaacc actacagcct
     1621 ggcgaccggc ctgtaccccg accaccatgg aattgtgaat aactcatttt ttgacaaaaa
     1681 gcttggttac tactccattg gtaacaggaa gagtgtggaa aatggggagt tctatcaggg
     1741 tgagcccatc tgggtaaccg ctgagaaaca aggcattagg acagcctctt actactgggt
     1801 aggaa
//
LOCUS       DAOOQX010000448         1802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_83053_length_1802_cov_3.095020, whole genome shotgun sequence.
ACCESSION   DAOOQX010000448 DAOOQX010000000
VERSION     DAOOQX010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83053_length_1802_cov_3.095020"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1802)
                     /gene="secD"
                     /locus_tag="PLX53_07300"
     CDS             complement(<1..>1802)
                     /gene="secD"
                     /locus_tag="PLX53_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOY72923.1"
                     /translation="NNALAEIQDATAKTDSIKSTSSKELTAKKDTVTDELLAKLKATS
                     TEDSLSATQADQTKKYPLFSLLQPNLYQDQPAPGPLVGYAHVKDTARVNEILRMPKIK
                     ALFPVDVKFLWGVKPPKWDKSESLYELIALKVTSRDGKAPLDGGVITDAREAFGETKG
                     SAEVDMSMNAEGARVWARMTGENIGRCIAIVLDNYVYSYPRVQSEIKGGRSQITGDFT
                     INEAKDLANILKSGKMPAPARIVQEELVGPSLGTESINSGMNSFILSFILVLLFMMVW
                     YSFKAGLVADIALFFNLFFIMGIMASFGATLTLSGIAGIVLTLGMAVDTNVLIFERIR
                     EELRSGKGTIPSVNEGYRNALSAIIDGNVTTLLTGIILYVFGTGPIRGFATTLIIGII
                     TSLFTGILISRLIQTWMINHNINIRYSVKFTEHAFRNLHIDFIGIRKYAYIFSGSLFL
                     IIFISLGVRGLNQGIDFTGGRSYVVRFDDAVSTTDIASDLKHVFNEAPEVKTFGGQNQ
                     VKITTKFKIDSKDENIENEMDSLLYVGLKPYLQDGTTLKDFQTTYKQSSIKVGPTIAQ
                     DIKRDAVIAVILALIVIFLYVLIRFRWWSYSTGA"
BASE COUNT          453 a          430 c          433 g          486 t
ORIGIN      
        1 tagcaccggt gctatagctc caccagcgga aacgaataag cacgtaaagg aatatcacga
       61 ttagtgccag gataaccgca attaccgcat cgcgcttgat gtcctgtgca atggtaggtc
      121 ctacctttat ggagctttgc ttgtaggtgg tttgaaaatc cttcagggtg gttccatctt
      181 gcaggtaggg ttttagccca acgtagagca gggagtccat ttcgttctca atattttcat
      241 cctttgagtc tattttgaac ttggtagtaa tcttcacctg gttctgaccc ccaaatgttt
      301 taacctcggg cgcctcgtta aacacatgct ttaggtccga ggcaatatcg gttgtcgaaa
      361 ccgcatcgtc aaaccttacc acatacgagc ggcctcccgt aaaatcaata ccctgattca
      421 acccccttac acccagcgat ataaatatta tcaaaaaaag tgaaccggaa aagatgtagg
      481 cgtactttcg tattccgatg aaatcaatat gtagatttct aaaggcatgc tcggtaaatt
      541 taacggagta ccggatgttg atgttatggt ttatcatcca ggtttgaata agcctggaga
      601 taagtattcc ggtgaaaagg gaggtaatga taccgatgat gagcgtggtg gcaaaacctc
      661 gaattgggcc tgtgccaaat acgtagagaa taattccggt tagcagggta gtaacgttac
      721 cgtcaataat ggcagagagg gcattcctat acccctcgtt gactgagggt atagtccctt
      781 ttcccgatcg taattcctcc cttattcgtt caaagatgag aacgtttgta tcaacggcca
      841 tacccagcgt tagcacaata cctgcgattc ccgaaagggt aagcgttgcc ccgaaagagg
      901 ccatgattcc catgatgaag aaaaggttga aaaacaaggc gatatccgca accaagcctg
      961 ccttgaagct gtaccatacc atcatgaaga gcagcacgag gataaaggag aggataaagg
     1021 agttcatacc tgaattaata gactcggttc ccagcgatgg gcccaccagc tcttcctgaa
     1081 caatgcgggc gggagccggc atttttccag actttaaaat gttcgcaagg tcttttgcct
     1141 cgttgatggt aaaatcaccg gttatttgtg aacgtccacc cttaatctcg ctctgcaccc
     1201 ttgggtagga gtaaacgtag ttatcaagta caatagctat acagcgacca atattttcac
     1261 cggtcatgcg cgcccaaact cttgcccctt cggcattcat ggacatgtca acctcggcgc
     1321 tgccctttgt ctctccaaac gcctctctgg cgtcggttat tacaccccca tcaaggggtg
     1381 ccttgccatc gcggctggtt accttgaggg ctataagctc gtagagcgac tcggatttat
     1441 cccattttgg aggttttaca ccccacaaaa atttcacgtc aacggggaaa agtgccttaa
     1501 tcttaggcat ccttagtatt tcgttaaccc tggctgtgtc ctttacatgg gcgtagccta
     1561 ccaaaggccc gggggcgggc tgatcctggt acaggttggg ttgaagcagg ctgaataacg
     1621 ggtatttttt tgtctggtcg gcctgagtgg ctgaaaggga atcctcggtt gaggtggctt
     1681 tcagcttggc cagcagctca tctgttacgg tatccttttt ggcggttagc tccttgctgc
     1741 tggtgctttt aatggaatcc gtcttagccg tagcatcctg aatctcagcc aacgcgttgt
     1801 tg
//
LOCUS       DAOOQX010000449         1801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_83129_length_1801_cov_2.979381, whole genome shotgun sequence.
ACCESSION   DAOOQX010000449 DAOOQX010000000
VERSION     DAOOQX010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83129_length_1801_cov_2.979381"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..724
                     /locus_tag="PLX53_07305"
     CDS             149..724
                     /locus_tag="PLX53_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOY72924.1"
                     /translation="MGRKIIVGVTGASGAIYARSLLEILGELSDQLEEVALIFSDNGR
                     AVWEFELGHIPPFSAPFRVYENNNLFAPVASGSAGFDTMFIAPCTMGTLGRIAAGTSS
                     DLICRSADVMLKERRRLIVVPRELPLSLIHIENMRTVTLAGGIIVPASPSFYSRPSSM
                     EELVRTVVFRMIDLAGLELPHYRWGEAKSDS"
     gene            678..1400
                     /locus_tag="PLX53_07310"
     CDS             678..1400
                     /locus_tag="PLX53_07310"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY72925.1"
                     /translation="MSCLITDGEKLKAIRSLINSNFFTMQGEEKVVSRYITNLIGMGE
                     GLTLDFKHSITDSKKIARSLAAFANTQGGTLLVGVKDNGSITGVDTDEEYYMVEAAST
                     LYCRPEVPFEVVKWNMNAKTVLEVKVKPSKLRPHKAPDKNGHYKAYIRIADENMVVTS
                     TQLKIWKAERSRKSIGLNLSNAQTALFEHFKQNPRITIDEFCKIGFLPATEAERILVN
                     LTVLGTIRYSGNPADEYFYPSV"
BASE COUNT          490 a          382 c          428 g          501 t
ORIGIN      
        1 taaatagtta gctaacgaca cgctaagccg gattggcgtg tgaacaaaat caacaagttg
       61 aaagtttttt tgatacattt gttggcagta taacaagtag aatgctgtag gcaaacgtcc
      121 ggactactta ctataggttg attttgacat gggacgaaaa attatagtgg gagtaacagg
      181 cgccagcggt gccatatatg cccgttcgct gctagagatt ttgggtgagc tttccgatca
      241 gctggaggag gtagcgctta ttttttccga taacgggcgt gcagtttggg agtttgagct
      301 tggccatatt ccaccttttt ctgctccttt ccgcgtttac gaaaacaaca acctgtttgc
      361 tcctgtggcc tccggatcgg ctgggttcga cacgatgttt attgcgccct gcaccatggg
      421 aaccttggga agaatagctg caggaaccag ctccgacctg atttgtcgat cagccgatgt
      481 tatgctcaag gagcgacgtc ggctgatcgt agtgccgaga gagctaccct tgagccttat
      541 acatattgag aacatgcgaa ccgttaccct cgcaggcggt attattgttc cggccagccc
      601 atcgttttat agccgccctt catccatgga agagctggtt aggactgtcg ttttcaggat
      661 gattgatttg gcagggcttg agctgcctca ttaccgatgg ggagaagcta aaagcgattc
      721 gtagcctgat aaattcgaat ttttttacca tgcaggggga ggagaaagtt gtgagtaggt
      781 acattactaa tttaattggc atgggggagg ggttgaccct cgatttcaag cattccatca
      841 ccgattcaaa aaagattgcc cgttcattgg ctgcatttgc aaacactcag ggcgggacgc
      901 tgctggttgg ggtaaaggat aacggcagca ttacgggtgt cgataccgat gaggagtact
      961 acatggtcga agctgcctca acactttact gcaggcccga agtacctttt gaggtggtta
     1021 agtggaacat gaacgccaaa accgttctgg aagtgaaggt aaaaccttca aaattacgcc
     1081 cccataaagc ccccgataag aatgggcatt acaaggccta catccggata gccgacgaaa
     1141 acatggtggt tacatcaacc cagcttaaaa tttggaaggc ggaacgttcg cgcaaatcca
     1201 tcggtcttaa cctatccaat gcgcaaacag ccctgtttga acatttcaaa cagaatccac
     1261 gcattaccat tgacgagttc tgcaaaattg ggtttctccc ggctactgag gcagagcgta
     1321 ttctggttaa cctcaccgtt ttaggaacaa taaggtattc aggtaatcct gctgacgaat
     1381 atttctaccc ttccgtctaa actttttatg cttattatgg ttttatggtc taaaggcaat
     1441 agctgcttac ggtttatctt ttgtagagtt catgttgacc tttcttcatt tttaatgaag
     1501 tacccaggca ggacacagtt tgcataagca cgtatgaaca accaggcgtt acttgcaagt
     1561 ccggtgaagc taagatttag gcgttacatt cgctcaaccc aaatgcctaa aaggcatgag
     1621 ctaaagtagc tgtaatcgga aacctgaaaa ttttgaacag atgaaaccat tatgatcagc
     1681 ataatttttt attagcgtca atttacaggg caatggtatt ttttagcgtg aaagacccca
     1741 attttttttt actttgtaaa aattttgaca aaggaaaaga ccatctctca gaaaattatt
     1801 c
//
LOCUS       DAOOQX010000450         1797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_83427_length_1797_cov_2.865672, whole genome shotgun sequence.
ACCESSION   DAOOQX010000450 DAOOQX010000000
VERSION     DAOOQX010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83427_length_1797_cov_2.865672"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            583..>1797
                     /locus_tag="PLX53_07315"
     CDS             583..>1797
                     /locus_tag="PLX53_07315"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOY72926.1"
                     /translation="MVRKIFFIPAGLILLFTSVVVLNSCAGKSTQGEDLSFAELSPAQ
                     RDEFINDLKSRTFRYFWEVVDTNTWQNDDRFPTYNFTSIAATGFALPSFIIGVENNFI
                     SRQQASERVLNTLEWLWSSKQGAEPDATGYKGFYYHFLSYGTGRRYDKVELSTIDTGL
                     LLAGILTCQTYFDGADKTEVRIRELADSIYLRVDWQWAMNGAANMSMGWTPEQGFIQS
                     TWNGYNEAMILLIMAIGSPTHPIDSTAWDSWCSTYKWDSFYGYEHVPFGPLFGHQYSH
                     QFIDFRGIQDSYMRSKGIDYFENSVRATLSNRAYCMENPLKFEGYSANMWGLTACDGP
                     AMAKIRANGRVVQFYDYRARSACSYEIIDDGTIAPTAAGGSIPFAPEECLSALYTMKT
                     MLGSNVYREYGFV"
BASE COUNT          511 a          378 c          413 g          495 t
ORIGIN      
        1 taatgcctgc aacgttcaac gattccagcc tttccgggca ctgcggagaa taccttttcc
       61 cccgttgggg cagaatacaa gggtaaaggt taatacatgg ataaaggtta aaggataaag
      121 gataaaaaaa atcaagcagg attcaagaac ctgtccgcct accggcggaa cttgtccggc
      181 tcctgacgga ttcaacattt agctaggggc attaacggca tgggtagcac tgacttagtc
      241 ataccctatg gttaggttat aaatcgtcta aattttatca taatgaatta aaaaccacaa
      301 aatcaatatg ttaatatttt ataggtgtaa ttaaatggtt tcgccgaaat cgttttcggc
      361 taaaaatatt tctataaatc ataattctaa agtatcagaa aaacagtata ataaagttta
      421 aatattaaaa atgacgtact gttaagcatg cttaaaaaat tcccttttgt tataaccgta
      481 gccgtgatga ggcggaattc tggacaaacg ttttatcgag tgttaacttt gagctaaatc
      541 aagttaaagg atataatgat ttgtaaattt tttgcttcaa ctatggttag aaaaattttt
      601 tttattccag caggcttaat actgttattt acctctgttg ttgttttaaa ttcatgcgct
      661 ggtaaatcaa ctcaagggga agatctttct tttgctgagc taagtcctgc tcaaagggat
      721 gaatttatta atgacttaaa aagtaggaca tttcgctact tttgggaggt ggttgacacg
      781 aatacctggc aaaatgacga ccgctttcca acctacaatt ttaccagcat tgctgcaacg
      841 ggttttgccc ttccctcttt tataataggt gtagaaaaca attttatttc ccgtcagcaa
      901 gcttcggaac gagtgctcaa caccctggag tggctttgga gttcaaagca gggagctgag
      961 cccgacgcaa ccggatataa aggcttttac taccacttcc tttcgtacgg gaccggcagg
     1021 agatatgata aggttgagct ttcgactatt gatactggct tgctcctggc cggaatactt
     1081 acctgccaga cgtacttcga tggggccgac aaaaccgaag ttagaatacg ggagcttgcc
     1141 gattccattt acctgcgcgt cgactggcaa tgggctatga atggtgcagc caacatgtcc
     1201 atgggctgga ctcccgagca aggattcata cagagcacct ggaacgggta taatgaggcc
     1261 atgattctac tcatcatggc tatcggctcc ccaacccatc ccatagactc caccgcatgg
     1321 gacagctggt gcagcaccta caaatgggat agtttttatg ggtatgagca tgtgcccttt
     1381 gggccactct ttggccatca gtactcccat caatttatcg acttcagggg aatacaggat
     1441 agctacatgc gttcaaaggg aattgactac ttcgagaact cggtaagggc taccctgagc
     1501 aacagggcct actgcatgga aaatccactc aaatttgagg ggtattcggc caacatgtgg
     1561 gggcttacgg cgtgtgatgg ccctgcaatg gccaaaatca gggctaatgg cagggtcgtg
     1621 cagttttacg actaccgggc aagaagcgct tgcagctacg agattatcga cgatggaaca
     1681 attgctccta cagcagcagg agggtcaatc ccatttgccc ccgaggaatg cctgagtgct
     1741 ctttatacca tgaagactat gctgggtagc aatgtttacc gggagtacgg ctttgtt
//
LOCUS       DAOOQX010000451         1789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_84005_length_1789_cov_2.356401, whole genome shotgun sequence.
ACCESSION   DAOOQX010000451 DAOOQX010000000
VERSION     DAOOQX010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84005_length_1789_cov_2.356401"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLX53_07320"
     CDS             <1..254
                     /locus_tag="PLX53_07320"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP carboxykinase; PEP carboxylase; PEPCK;
                     catalyzes the phosphorylation and decarboxylation of
                     oxaloacetate to form phosphoenolpyruvate using ATP;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOY72927.1"
                     /translation="IINAILNGSIEESEFELLPVFNLQVPLRVNGVNPHLLNPQKSWA
                     NPGDWDKAARNLAGMFIKNFENFTDNEWGKSLVKAGPHI"
     gene            658..1314
                     /gene="plsY"
                     /locus_tag="PLX53_07325"
     CDS             658..1314
                     /gene="plsY"
                     /locus_tag="PLX53_07325"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOY72928.1"
                     /translation="MEILSKIAIVFLAYLMGSIPTSVWVGKIFYGVDVREHGSGNAGA
                     TNTIRVLGLLPGIVVFIVDVLKGYLAVRLLYLTDFYIPKTGMFINFQLILGIAAMIGH
                     IFPIFAQFRGGKGVAVLSGVVFALHPYATLIVFGIWIVSVIITGYVSLSSMIAGFSFP
                     LILIFVYRTTNPSLILFAFILAILMLFTHQKNIERLFKGEESKFHLKKKPHNEPDKPK
                     "
     gene            1376..>1789
                     /locus_tag="PLX53_07330"
     CDS             1376..>1789
                     /locus_tag="PLX53_07330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOY72929.1"
                     /translation="MQIFYAPNITGSSYTLPEDEARHCLRVLRMRVGDEVELVDGKGN
                     LFTGRIANPDLKECTLEIVRKASNFGERHFYLHIAIAPTKNADRFEWFAEKVTEIGVD
                     EITPLLCERSERKSVNSERIERIVISAMKQSEKAYL"
BASE COUNT          465 a          372 c          384 g          568 t
ORIGIN      
        1 ccataatcaa cgctatcctg aatggctcca ttgaggagtc cgagtttgag ctgctacctg
       61 tttttaacct tcaagtccca ttaagggtta acggggttaa cccacattta ttaaatcccc
      121 agaagtcgtg ggcaaatcca ggcgattggg ataaagcagc cagaaatttg gctggaatgt
      181 tcattaaaaa ttttgaaaat tttaccgata acgagtgggg aaaaagcctg gttaaagcgg
      241 gtcctcacat ctaaacttac taagtaagtt tcggtttata cactacttgc tgcctaactc
      301 caccacatga ctgattatgt atgggcttgt ggtggtttta tttgacacgg gttttaatac
      361 tcctggctag caataagcta tgcctcaggt ttctaataat caacagcctt aaggtaaatc
      421 tcctatggct tgtattgaag atgtcctaat tttggtaatt tgtgtgagcg attagcctag
      481 ctcaaccctg ggcgttttat aacatttttt aacacttccc ccttgctttc aaatagctga
      541 catgaccagc tttaacctat aattttttcc atgaatgttt cggtttaaag gtggtatgag
      601 taacttttaa cacatattct tttgccctct taaagcaata ggcttatctt tgcctttatg
      661 gaaattttgt ccaaaatagc cattgtcttt ctggcttacc tcatgggctc cattcctacc
      721 tccgtgtggg tgggaaaaat tttttatggg gttgacgttc gtgagcatgg cagcggcaat
      781 gctggggcga ctaatacaat acgggtgctt ggccttctgc ctggaatcgt agtttttatt
      841 gttgatgttt taaagggtta ccttgctgtt aggctgcttt acctcaccga tttttacata
      901 cctaaaacag gcatgttcat taatttccag ctcattcttg gaattgccgc catgattgga
      961 catattttcc caatttttgc ccagtttcga ggtggaaagg gggtagccgt tctttcgggg
     1021 gttgtttttg ccctgcatcc ctatgccact ctgattgttt tcgggatatg gattgtatcg
     1081 gttatcatta ccggctacgt ttcgctaagc tccatgattg ctggtttttc atttccactt
     1141 attttgatat ttgtttaccg caccacaaat ccctccctga tacttttcgc ctttattctg
     1201 gccatcctta tgctattcac ccatcaaaaa aatattgaaa ggctgtttaa gggggaggaa
     1261 tcaaaatttc acttgaaaaa gaaaccacat aacgagcccg ataagcctaa atagccagtt
     1321 tatgaattat tttatcggtt ttatttgacc taacctgaat agccctataa ggcaaatgca
     1381 aattttttat gccccaaata tcacgggaag cagctatacc ctgcctgaag atgaggcacg
     1441 acactgccta agggttctta ggatgagggt gggcgacgag gttgagctgg ttgacgggaa
     1501 aggaaatctt tttaccggca ggatagccaa tcccgaccta aaggagtgta ctctggaaat
     1561 tgttcggaaa gccagcaatt tcggtgagcg ccacttctac cttcacatcg caatagctcc
     1621 aactaagaat gccgatcgtt ttgaatggtt tgccgagaag gttacggaaa ttggagttga
     1681 tgagatcaca cctcttcttt gcgagcgttc ggagcgcaaa tcggtaaatt ctgaaagaat
     1741 tgagagaatt gtgatttcgg ccatgaagca atccgaaaaa gcctatcta
//
LOCUS       DAOOQX010000452         1784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_84316_length_1784_cov_3.337189, whole genome shotgun sequence.
ACCESSION   DAOOQX010000452 DAOOQX010000000
VERSION     DAOOQX010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84316_length_1784_cov_3.337189"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLX53_07335"
     CDS             complement(<1..497)
                     /locus_tag="PLX53_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOY72930.1"
                     /translation="MRIKFFSISNRYRYVFIDLDRTLWDFERNSLEALHELFSEYKLD
                     SVVADATTFSRTYVKHNNYLWNSYCKGELTKEVLRYKRFDATLSELGVNNHQLAAKLG
                     EEYLRILPLKTILIPNAKELLDYLAPKYGLMILSNGFHEVQVDKLKRCGLNHYFDRVV
                     TSELSG"
     gene            831..>1784
                     /locus_tag="PLX53_07340"
     CDS             831..>1784
                     /locus_tag="PLX53_07340"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOY72931.1"
                     /translation="MNFSELNLNPHILEGISTMGYGNPTPIQEQAIPVILQGRDLIAC
                     AQTGTGKTAAFLLPILGKIAEKPTTEGYINTLIIVPTRELALQIDQQVEGFAYFISTS
                     SIAVYGGNDGNSWDRQRMALEQGADIIVATPGRLLQHIQMGYVKLDRLEHLILDEADR
                     MLDMGFFDDITAIISQLPTRRQTLMFSATMPPKIRQLAKTILDNPVEISLSVSKPVES
                     ILQAVYMVEDSEKIGLLQSLVAGKEYVQSVLVFASKKMEVKDIERKLVRESIMARAIH
                     SDLEQSQREQVLRDFRNRKFQVLVATDIVARGIDIEDIDLVV"
BASE COUNT          503 a          384 c          416 g          481 t
ORIGIN      
        1 cccgaaagtt ctgaggttac taccctgtcg aagtagtgat tcagcccaca ccttttcagc
       61 ttatcaacct gcacttcatg aaagccattg ctcagaatca taagcccgta ctttggcgca
      121 aggtaatcga gtagctcctt ggcatttgga attaggatgg tttttaaggg taatatgcgg
      181 agatactcct cgccaagttt ggctgcaagt tggtgattgt ttactcctaa ctcggaaagt
      241 gtggcatcaa atcgtttgta tcgcagcacc tccttggtta gctccccttt gcagtagcta
      301 ttccatagat agttgttgtg ctttacatac gtgcgggaaa atgttgtggc atcggcaact
      361 accgagtcaa gcttgtattc cgaaaacagc tcatgtagag cttcgaggct gtttcgctca
      421 aaatcccaaa gagttctatc caaatcaata aaaacgtacc tgtacctatt gctaatgctg
      481 aaaaatttaa tcctcataac ccgctccttt tcgctgcaaa agtagtaatt tacaaaatta
      541 tagctaccag gttacttccc cacaattagc cagaactaaa ggcataagaa aacggagtag
      601 ggcaatattt ataaatgaaa taattcagaa tggtagcggt ttacagagag tatttaatga
      661 atattctgac caaaaaaggg gggggtgaaa atttatgata gcatgaaata gcgcttctgc
      721 gcgtctttta ggttgaatat ttaacccagc accaggcagg gtagcagcac tttttcacct
      781 aaatttgaaa acagtatctt tgcatccgaa tacatcaaca aacattaaac ttgaatttta
      841 gcgagttaaa ccttaatcct cacatacttg aggggatttc gacaatgggg tatggaaatc
      901 ctaccccaat acaggagcag gcaattccgg taattcttca ggggcgtgat ttaatagcct
      961 gtgcacaaac cggaactggg aaaacagctg cttttttgct gcctattctg ggtaaaatag
     1021 ccgaaaagcc taccactgaa ggctacataa acaccctgat aattgttcct acgcgtgaac
     1081 ttgccctgca gattgaccag caggtagagg gttttgccta cttcatttca accagctcca
     1141 ttgccgttta cgggggtaat gatggcaaca gctgggatcg gcagcgcatg gccctggagc
     1201 agggggctga tattatagtt gcaactcccg gaaggctcct ccagcatatt caaatgggct
     1261 acgttaagct ggataggctg gagcatttaa ttcttgacga ggccgaccgc atgctcgaca
     1321 tgggtttttt tgacgatatc acggcgatta tctcccagct gccaacccga aggcaaacct
     1381 tgatgttctc tgcaaccatg cctcctaaaa tccggcagct ggccaagact atccttgaca
     1441 atccggttga aataagcctt tcggtctcta aacctgtcga aagtattttg caggcggttt
     1501 acatggtgga ggattcagaa aaaatcggat tgctacagtc gttggtggcc ggtaaggagt
     1561 acgtgcaaag cgttctggtt tttgcttcta aaaaaatgga agtaaaggac attgagcgca
     1621 agctggtaag ggaaagcata atggcaaggg caattcattc cgaccttgag caaagccagc
     1681 gtgaacaagt gcttcgggat ttccggaacc gaaaatttca ggtgctggtg gctaccgata
     1741 ttgttgcgcg tggaattgat attgaggaca tagacctggt ggtt
//
LOCUS       DAOOQX010000453         1779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_84711_length_1779_cov_3.212297, whole genome shotgun sequence.
ACCESSION   DAOOQX010000453 DAOOQX010000000
VERSION     DAOOQX010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84711_length_1779_cov_3.212297"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..702)
                     /locus_tag="PLX53_07345"
     CDS             complement(34..702)
                     /locus_tag="PLX53_07345"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOY72932.1"
                     /translation="MIKVAMQQDTYKHKGLRKRLIEEIESKGITDKQVLGAMLRVPRH
                     FFMESSFLKFAYSDQAFPIGYGQTISQPFTVAFQTQLLQVEHMNKVLEVGTGSGYQAA
                     ILMELGVRLFTIERQRELYLNAKSLLEEMGYHPSFHFGDGYKGLPGYSPFDRIIVTAG
                     APTIPDELVDQLAVGGRMVIPVGDENGQVMTLVEKISSTEVRKTTHGNFIFVPLLKGV
                     EKSK"
     gene            935..>1779
                     /locus_tag="PLX53_07350"
     CDS             935..>1779
                     /locus_tag="PLX53_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72933.1"
                     /translation="MLKLGFFTDNTQLAELVGDLGRQANIEVEGIWAEPLLNGDDKRN
                     FSNCFSDPEELYGSIDVGVISISSSELFMDIVTAAIKNGVHPLVVGLPDLPLSTYNQL
                     SLLSAEMGVELGFSHIYHRFSGFNYPLVSPIIAHLTRGITMNQLNQFEMAATLRFDIA
                     TILSLTTSEVRRIRTYVLPQSGECPSCMMVFIDFFNNSAITYNLTLGAKRNFLELRLN
                     SIGEAIELYEDEIKLATSSLGGMISTSIFLDDLNNFTGCIGIQREPLLGISFVMKILS
                     AVEAIS"
BASE COUNT          478 a          371 c          386 g          544 t
ORIGIN      
        1 ggttacatgt gttggtaggc atagataggc ttgttacttg cttttttcca ctccttttaa
       61 caacggaaca aagataaagt ttccgtgggt agtctttctt acttcggtgc tgctaatttt
      121 ttcaactagc gtcatcacct gaccgttttc atccccaacc ggaataacca ttcttccacc
      181 aaccgccagc tggtcaacaa gctcatcagg aatggttggc gcacctgcgg taacaataat
      241 tctgtcgaaa gggctatagc ccggtaatcc cttataccca tcgccaaaat gaaagctggg
      301 gtgatagccc atttcctcca gcagcgattt ggcgttaagg tatagctctc tttgcctttc
      361 gatggtgaac agcctaacgc ccagctccat aaggattgca gcctggtaac ccgagcctgt
      421 tcctacctcc agcaccttgt tcatgtgttc aacctgaagg agctgggttt ggaacgccac
      481 ggtaaagggt tgagatatgg tttgcccata tccaattggg aaagcctggt cgctgtaggc
      541 aaactttagg aaggaacttt ccataaaaaa atgacgggga acccggagca ttgccccaag
      601 cacctgctta tccgtaatcc ccttcgattc aatttcttca attaaccgtt tccgtagccc
      661 tttatgctta taggtgtcct gctgcatcgc aacttttatc aagttaaaat ggtcgttcaa
      721 atatacatct aaaagggcat ttgaaaattt taaatgtaat agacagcaaa agagatattt
      781 acactttttt tgctatacct aattttttta tgccagcttg ttgtatcatg gtgttgctat
      841 ttatttgaac cattgtagtt gataagttac ccgactaaaa tgttggagat gacggtaaaa
      901 tctataagtt tgcaactatt cacccattca acatatgctt aaacttggtt tttttactga
      961 taacacgcag ctggctgaat tggtaggaga tttagggaga caagccaata tagaagtgga
     1021 ggggatatgg gcagaacctc ttctaaatgg tgacgacaaa cggaattttt ccaattgttt
     1081 cagtgatcct gaagagctat atggcagtat tgatgtggga gttatttcca tttcatcatc
     1141 cgagctcttt atggatatag ttacggcagc cattaaaaat ggagtgcatc ctctggttgt
     1201 tggactgccc gaccttccgc tttctacgta taatcagctg agcctgctta gcgctgagat
     1261 gggggtggag cttggtttta gccatatcta ccaccggttt agcgggttta attatccctt
     1321 agtcagccca ataattgccc atctgacacg tggaataacc atgaaccagc ttaatcaatt
     1381 tgaaatggcg gctacgctta ggttcgatat agccactatt ctatcgctaa ctacaagtga
     1441 agtaagaaga attagaactt atgtgctgcc ccaatcgggt gaatgtccaa gctgcatgat
     1501 ggtttttatc gatttcttta acaactcagc aattacctat aacctgacgt taggggctaa
     1561 aagaaatttt ttggaacttc ggcttaactc aattggtgag gcaattgaac tttatgagga
     1621 tgagataaaa cttgcaacaa gctcccttgg cgggatgatt tcgactagca tttttttaga
     1681 tgatttaaac aactttacgg gttgtattgg catccaaagg gagccgttgc tgggcatttc
     1741 atttgtaatg aaaatactta gtgcggttga ggcgatttc
//
LOCUS       DAOOQX010000454         1775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_85019_length_1775_cov_3.276163, whole genome shotgun sequence.
ACCESSION   DAOOQX010000454 DAOOQX010000000
VERSION     DAOOQX010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85019_length_1775_cov_3.276163"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..>1775)
                     /gene="ggt"
                     /locus_tag="PLX53_07355"
     CDS             complement(154..>1775)
                     /gene="ggt"
                     /locus_tag="PLX53_07355"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOY72934.1"
                     /translation="TGLNFATRSEVIASHGMACTSQPLATQVAIDILKKGGTAVDAAI
                     AANAMLGLVEPTGSGIGGDLFAIVWDAKTQKLYGLNASGRSPKSLSLEYFKEHGIKHI
                     PAIGPLPVSVPGCVDGWFELHSRFGRLPMKVILEPTIGYAREGFPVSELIAYYWKGNA
                     ERLKQFEGFADVYLPNGKAPAKGEIFKNPYLANTLEMIAKNGRDEFYRGGIARTIDDY
                     MKRNGGFLSYNDLASHRSEWVEPVSTSYRGYDVWELPPNGQGIATLEMLNILENFDIA
                     SMGFGSADYMHLFVEAKKLAFEDRARYYADPDFTDVSYMSLLSKDYAKNRAALIDMNR
                     AARSYDAGELQTGNTVYLTVADSEGNMVSLIQSNYRGMGSGMTPTGLGFVLQDRGELF
                     SLEEGHPNVFQPGKRPFHTIIPGFITKSGRPWVSFGVMGGGMQPQGHVQVIVNLIDFG
                     MNLQEAGDAPRIQHEGSSEPTGERMNDGGNVFLESGFDYQEIRKLLGKGHRISYSLDG
                     YGGYQAIMYDVRNRVYVGASESRKDGQAAGY"
BASE COUNT          442 a          495 c          389 g          449 t
ORIGIN      
        1 gcttcacctc atgcttgcgg agtaaccctg cttggctcac cccgaaccga tggatacgtg
       61 ctcaacgatc ccgatggcaa ccccgatcct atcattcaca agtagttata ttgcgtcact
      121 aatcaaaaaa gaggctttaa gcctcttttt tgattagtat cctgctgctt gcccatcttt
      181 tctcgactcc gaagccccaa cgtaaaccct attccgtaca tcgtacatta tagcctggta
      241 tccgccgtaa ccgtcaaggc tgtatgaaat cctgtgtcct ttgccaagaa gtttacggat
      301 ttcctgatag tcaaagccag attccagaaa aacgttaccg ccatcgttca tcctctcacc
      361 cgttggctcg gagctaccct cgtgctggat tcgcggtgca tcgcctgcct cctgtaagtt
      421 cataccaaag tcgataaggt taacaataac ttgaacatgc ccctggggtt gcattccgcc
      481 acccataacc ccaaagctta cccatggcct tccactcttt gtaataaaac caggaataat
      541 ggtgtgaaat ggccttttcc cgggttggaa aacattggga tgtccttcct caagcgagaa
      601 cagctcaccc ctgtcctgca gcacaaaacc cagacctgta ggagtcattc ccgaacccat
      661 tcccctgtaa ttgctctgaa tgagcgaaac catgtttccc tcgctgtcgg caacggtgag
      721 gtaaacggtg ttgcctgttt gcaactcacc cgcatcgtaa cttcttgctg cacggttcat
      781 atcaataagg gcagcacgat tttttgcgta gtccttcgaa agcagactca tatatgaaac
      841 gtcagtaaaa tcaggatcgg catagtaacg tgccctatcc tcgaaggcga gtttctttgc
      901 ttcaacaaaa aggtgcatgt agtcagccga gccaaagccc attgaggcaa tatcgaaatt
      961 ttcgaggatg tttagcatct cgagcgttgc aatgccctgg ccgtttgggg gaagctccca
     1021 aacatcgtag ccccggtatg aggttgaaac gggttcaacc cattccgagc ggtgtgaggc
     1081 taaatcgttg taggacaaaa atccaccatt cctcttcatg taatcatcaa tggtccgggc
     1141 aatgccgcca cggtagaatt cgtcacgacc attctttgca atcatttcca aggtatttgc
     1201 caggtaggga ttcttgaata tttccccctt tgcaggcgct ttcccgttgg gcaggtaaac
     1261 atcagcaaaa ccttcgaact gcttaagcct ttcggcatta cccttccagt agtaggcaat
     1321 tagctctgat acaggaaatc cctcccgggc atagccaatt gttggttcaa ggattacctt
     1381 catgggcagc ctgccaaatc tactgtgcag ctcaaaccag ccatccacgc aaccaggcac
     1441 cgataccggc agcgggccaa tggcagggat atgctttatt ccatgctcct tgaaatactc
     1501 aagtgatagc gacttgggcg aacggccgct ggcatttaac ccatagagtt tttgcgtttt
     1561 ggcatcccat acaatggcga aaaggtcacc cccaatgccc gaaccggttg gttcaaccag
     1621 accaagcatg gcattagctg ctatggcagc atccacagca gttccgccct ttttcagaat
     1681 atctatggcc acctgagttg ccaacggttg gctggtacag gccataccgt gactcgcaat
     1741 cacctccgaa cgggtggcaa agttaagccc tgtca
//
LOCUS       DAOOQX010000455         1775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_85035_length_1775_cov_2.700000, whole genome shotgun sequence.
ACCESSION   DAOOQX010000455 DAOOQX010000000
VERSION     DAOOQX010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85035_length_1775_cov_2.700000"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PLX53_07360"
     CDS             <1..566
                     /locus_tag="PLX53_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOY72935.1"
                     /translation="LADSSNLAVLGICLGMQSINVAAGGTLYQDIPTDIYHLSTVESV
                     LAQEINQQHRNYNCSYGYEEQLLGGTFHQVEFSDELFSSIAAGKKPFVLSSHHQCLKM
                     LGDNIRVAGYSADGKVVEAIRHIKYKNVVGVQFHPEASLLYDRNELFLITPNQQSGQS
                     YLNLYPGESGENFHREFWRWFSALLVK"
     gene            complement(627..1460)
                     /gene="pyrF"
                     /locus_tag="PLX53_07365"
     CDS             complement(627..1460)
                     /gene="pyrF"
                     /locus_tag="PLX53_07365"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOY72936.1"
                     /translation="MTPEQLFDQIRLKRSFLCVGLDTELTKIPEFLLTKDDPIFEFNK
                     AIVDATHDLAIAYKLNLAFYEVHGATGWNSLVKTIKYIRRFYPSMFIIADAKRGDIAS
                     TARMYANAYFKSLDCDAVTVSPYLGHDSVTPYLNFEGKWTILLAITSNDGFRDFQLAV
                     NRDTGNFMFEDVVAKSLQWGNEANIMYVVGATHPEYFAAVRKIVPNHFLLVPGVGEQG
                     GSLEKVAEHGLNNRCGLIVNSSRGIIYADITSRFTSAARARALKLQQQMESILTAAKL
                     V"
     gene            complement(1473..>1775)
                     /locus_tag="PLX53_07370"
     CDS             complement(1473..>1775)
                     /locus_tag="PLX53_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOY72937.1"
                     /translation="QKSQLKNFDKALAELRTRLYNLEYQKYLDNLSSKRKTMVSTGDR
                     SAKIRTYNYPQGRVTDHRINLTLYNLSAIMDGDIQELIDKLQLAENAERLKAESTD"
BASE COUNT          471 a          404 c          431 g          469 t
ORIGIN      
        1 tgctggctga tagctcaaac cttgctgtgc tgggaatttg cctgggaatg cagagcatta
       61 acgtggcagc cggaggaacg ctttaccagg atattccaac cgatatctac cacctttcaa
      121 ctgttgaatc ggtgctggca caggaaatca accagcagca ccgtaactac aactgcagct
      181 atggttacga agaacagctt ctcggtggaa cttttcatca ggtagagttt tcggatgagc
      241 tgttcagctc aatagctgcc ggtaaaaagc cctttgtgct gagtagccac caccagtgtt
      301 tgaaaatgct aggcgacaac attagggtag caggatattc agctgacggt aaagtggtgg
      361 aggcaatacg ccatatcaag tataaaaacg tggttggtgt tcagtttcac cccgaggcat
      421 cacttctgta cgacaggaat gagctgtttt tgattacccc aaaccaacag tcaggccaat
      481 cgtacttgaa cctttatcct ggggaatccg gtgaaaattt tcacagggag ttctggagat
      541 ggttttcggc cctgcttgta aagtaaaaaa tccgtattgt ggctgtagag cattgaatat
      601 ctaccaacgt gtaaaatgaa aaagggctaa acaagttttg ccgcagttaa aatagattcc
      661 atctgctgct gcagctttag ggcacgcgct ctagctgcgc tcgtaaatcg gctggtaata
      721 tcagcataaa taatgcccct tgaagaatta acaattaacc cgcagcggtt atttaaacca
      781 tgttcggcaa ccttttcgag gctaccaccc tgttccccaa cacccgggac aagcagaaaa
      841 tggttaggaa caatttttcg tacagcggcg aaatactcgg gatgagttgc cccaaccacg
      901 tacattatat tagcctcgtt gccccactgc aacgacttgg caacaacatc ctcaaacata
      961 aagttacctg tatccctgtt aacggccagc tggaaatcgc gaaagccatc gttggaggtg
     1021 atggccaaca ggatagtcca cttgccctca aaattcaggt atggcgtaac ggaatcgtga
     1081 cccaggtatg gcgaaacggt aaccgcatca caatccagac ttttaaagta ggcatttgca
     1141 tacatgcgtg cagtgctggc aatatcgccc cgtttggcat cggcaatgat gaacattgag
     1201 gggtagaagc gtcgaatgta cttgatggtt ttaaccaggc tattccatcc ggtagcgcca
     1261 tgcacctcgt agaacgccag attcagctta taggcaatgg ccaaatcgtg agtggcatca
     1321 acaattgcct tgttaaactc aaaaatcggg tcatccttgg taaggaggaa ctcgggaatt
     1381 tttgtaagct ccgtgtccaa ccccacacat aggaaactcc gtttcaatct tatctggtcg
     1441 aatagctgtt cgggagtcat tctggctact ggttagtctg ttgattcggc ttttagcctt
     1501 tcggcattct cagctagctg aagcttgtca attagctcct gaatatctcc atccatgatg
     1561 gcagagaggt tgtaaagggt aaggtttatc ctatgatcgg taactcgtcc ctgcgggtag
     1621 ttgtaggttc ggattttagc cgatcggtcg ccggttgaaa ccatggtttt acgcttggac
     1681 gaaaggttgt cgaggtactt ttggtactca aggttataca agcgggtacg tagctcggcc
     1741 aacgccttgt caaagttttt aagctgtgat ttttg
//
LOCUS       DAOOQX010000456         1766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_85710_length_1766_cov_3.059614, whole genome shotgun sequence.
ACCESSION   DAOOQX010000456 DAOOQX010000000
VERSION     DAOOQX010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85710_length_1766_cov_3.059614"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..535
                     /locus_tag="PLX53_07375"
     CDS             398..535
                     /locus_tag="PLX53_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72938.1"
                     /translation="MRRRKLIKSGMLVALLAMVLVAGSCSKKTCPAYSDTNKAQVINR
                     A"
     gene            complement(689..>1766)
                     /gene="trkA"
                     /locus_tag="PLX53_07380"
     CDS             complement(689..>1766)
                     /gene="trkA"
                     /locus_tag="PLX53_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOY72939.1"
                     /translation="LGAKKVIARIDRYEYLQPNNKEIFLNMGIDSLIYPERLAAREVI
                     TLLGQTSSTEFVDFSGGKLSLLVFRLEEDSPVLDKTLFQATRNIQNLEYRAVAIAREG
                     ETIIPRGNDQFKLNDLVYVIANQAGIKDMLEYTGKENIDINNLMILGGSRIAIHLAGE
                     LENSMNVKIIEVDREKSQQLAEQFKNALVINGDGRDTDLLMEEGMQYMDAFVAVTGNS
                     ETNILSCAIAKSLGVKKTIAEIENMSYIRLAESIGVDTVINKKLITASRIFRYTMSTD
                     VSAIKCLTGSDAEVLEFIVKPGSPVTKGKLCEISFPKDAIIGGVVRGSTAFIAKGDSE
                     IKPYDRVVVFALPSAINRVGRFFN"
BASE COUNT          495 a          411 c          379 g          481 t
ORIGIN      
        1 caaccagggg aaatcaacta cccgtaagct ggttaagcag taaccaacca aaataactac
       61 aaaacccccg taacttgggg gttttgtttt ttacataagt gaacttctat tttattcgtc
      121 aatatttttt attcactggt caaggtattt taaacctaag tcaaatatat tttagtaact
      181 tgcgtaatat ttctttattg caacaatatt aaagcagcat tgttacttag ggtaatacag
      241 gggtatccta aaaatgaaaa agaaaataac tccaattgtt attgggctgc tgttctgcct
      301 gatgatgctg aactcctgcc acaacaacat accgtgccct gtatatgcag atgtggatat
      361 ttgtgaaaaa actggtaact gattaataac taataatatg cgaagaagaa aacttattaa
      421 atcggggatg ttggttgccc tgctcgccat ggtactcgta gctggttcgt gcagcaagaa
      481 aacgtgtcct gcctatagcg atacaaacaa agcacaggtt atcaacaggg cttaagtccg
      541 atggttagcc tgatttaggc atcagataat aatatcgttt aaaaaaatat cctctcgaac
      601 gctttagggg ttacctgaac acctgaagcg tttagttata cacttaatgt tagttgggaa
      661 aacttctaca agtccaaccc gaaaactgct agttgaagaa acgcccaacc ctgttaatag
      721 ccgaaggaag ggcaaaaacc accacccggt cgtagggttt aatttcggaa tcgcccttgg
      781 caataaacgc cgtgctaccc ctaacaaccc cgccaataat ggcgtccttg ggaaaagaaa
      841 tttcgcaaag cttaccttta gtaaccggag aacccggctt aacgatgaac tccagaacct
      901 cggcatcgga accggtaagg catttgatgg cggacacgtc ggtgctcatg gtgtagcgga
      961 atatgcggct ggctgtaatc agctttttat tgataaccgt gtccacccca atgctctcgg
     1021 ccaaccgtat atagctcatg ttttcaattt cggcaatagt cttcttcacc ccgaggcttt
     1081 ttgcaatggc gcacgaaagg atgttggttt cggagttacc ggtaaccgcc acaaatgcat
     1141 ccatgtactg cataccctcc tccataagca ggtcggtatc gcgtccatca ccgttaatca
     1201 ccagcgcatt cttaaattgt tcggccagct gctgactctt ttcacggtcc acctctataa
     1261 tcttaacatt catggagttt tccagctcac cggccaggtg aatggcaatt cggctaccgc
     1321 caagaatcat caggttgttg atgtcaatat tctcctttcc ggtgtattca agcatatcct
     1381 taatgccagc ctggttggca atgacgtaaa ccaaatcgtt cagcttaaac tggtcgttac
     1441 cccggggaat aatggtttca ccttcgcggg caatggcaac cgccctgtat tctaaatttt
     1501 gaatgtttcg ggtggcctgg aatagggttt tatccaaaac cggggaatct tcttcgaggc
     1561 ggaaaaccag caacgatagc ttgccacccg aaaaatcgac aaattcggtg gagctggttt
     1621 gacccaacag ggtaattacc tcacgggcgg ctaacctctc cgggtagatt agggaatcga
     1681 tccccatgtt aaggaatatc tccttattgt tgggctgaag atactcgtag cggtcaatgc
     1741 gggctatcac ctttttagcc cccagg
//
LOCUS       DAOOQX010000457         1762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_86007_length_1762_cov_3.409490, whole genome shotgun sequence.
ACCESSION   DAOOQX010000457 DAOOQX010000000
VERSION     DAOOQX010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86007_length_1762_cov_3.409490"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(376..>1762)
                     /locus_tag="PLX53_07385"
     CDS             complement(376..>1762)
                     /locus_tag="PLX53_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOY72940.1"
                     /translation="LASVLIEEPDILILDEPTNHLDLDMVEWLEDYLIHGQQTLLMVT
                     HDRYFLDRVCGCILEIDNQSLFRYKGNYSYFVEKRQDRINALASEVEKANNTLRYESE
                     WIHRMPKARGTKSKYRIDAFYKLREKTLLQNLESPMEINISASRLGKKVMEVKNISKA
                     FGSKVILRDFSYTFNRFEKLGIVGRNGTGKTTFLNIITGNTLPDSGLVDIGSTVAIGY
                     YKQQDVSFPNNARVIDIARDIAEVVTIADGKTITVSQFLNMFLFPPEKQHDFVQKLSG
                     GEKRRLYLLTVLMRSPNFLILDEPTNDLDIQTLCILEEYLKSFPGVVVVVSHDRFFMD
                     KVVDGLLIFEGAGEVSGFPGSYTELREWTLEQQNDCPQGKPSQKSVKTGSSRVRIKQR
                     LSFAQKKELEHLTSEIELLEREKLELEQQLSSGMLDASKLQEKSEQYGHLLERLGNIE
                     MRWLELSELEG"
BASE COUNT          468 a          424 c          345 g          525 t
ORIGIN      
        1 aagtgccagg gcaatctcct tgcctatgcc tgacgaggct cctgttaccc aagcaatgcg
       61 atttctaaag tttaccatgg tatgtgttct tctctaacgg gtttcagaaa cccttcagaa
      121 attttgagtg gcaaatttag ctctttttac atttgaataa aaggaagcag gggaggcaat
      181 ccaaagcaca gccgtactca aatgttccat tttgagaaat ttttacatta acttcattcc
      241 tgctatttcc caatgctcac acaaaaataa acataaaacg tttacctgct tgctttctcc
      301 cacctgattt tccagtctaa aaacaactat tatccagtaa tttcaaccaa gaggttggta
      361 ctttaatgtg gctacctaac cttcaagctc gcttaactct aaccatcgca tctcaatatt
      421 tcccagcctt tcgagtagat gtccatactg ttccgatttc tcctgcagtt tgctggcatc
      481 cagcattccg ctactcaact gctgctccag ctctagcttt tccctttcaa gcagctcgat
      541 ttcggaggtt aggtgttcta actccttttt ttgggcaaat gaaagccgct gcttaatcct
      601 caccctgcta ctcccggtct ttaccgattt ttgagaaggt ttgccctgcg ggcaatcatt
      661 ctgctgttca agagtccatt ccctcaattc ggtgtacgat ccggggaaac cgcttacctc
      721 gccggcgccc tcaaatatca gcaggccatc aacaacctta tccataaaaa aacggtcgtg
      781 agaaaccaca actacaactc cgggaaagct tttaagatac tcttcaagga tgcaaagggt
      841 ttgaatgtcg aggtcgttgg tgggttcatc gaggataaga aagttagggc tacgcataag
      901 aacggttaaa agatataacc tgcgtttctc ccctccgctt agtttttgga caaaatcgtg
      961 ctgcttttcg ggagggaaaa ggaacatgtt gagaaattgc gaaacggtaa ttgttttgcc
     1021 atcggcaatg gtaaccacct cggcaatatc tctggcaatg tctattaccc tggcattgtt
     1081 gggaaagcta acatcctgct gcttatagta gccgatagct accgtgctgc caatgtcaac
     1141 caacccgcta tcgggaagag tattacctgt gataatgttg agaaaagttg ttttaccggt
     1201 tccatttcta ccaacaattc ccagcttctc aaacctgtta aatgtatagc tgaaatccct
     1261 gagaataacc ttgcttccaa aagccttaga tatgttttta acttccataa cctttttccc
     1321 taatcgggag gcactgatgt taatttccat cggtgattcc aagttttgca gcaaggtttt
     1381 ttcccttaac ttgtaaaaag catcaatacg atacttggac tttgtgcccc tggcctttgg
     1441 cattcggtgt atccattcac tctcgtatct tagcgtgtta ttggcttttt caacctctga
     1501 agcaagggcg ttaatcctat cctgccgttt ttccacaaag taggagtagt ttcctttgta
     1561 ccggaacaag gactggttgt caatctcgag aatacaaccg caaaccctgt caagaaagta
     1621 cctgtcgtgc gtaaccatca aaagggtttg ctgcccgtga attaaatagt cctccagcca
     1681 ctcaaccata tccaaatcaa gatggttggt aggttcgtcg agtataagaa tatcaggttc
     1741 ttcaatgagg actgatgcaa ga
//
LOCUS       DAOOQX010000458         1754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_86596_length_1754_cov_3.414361, whole genome shotgun sequence.
ACCESSION   DAOOQX010000458 DAOOQX010000000
VERSION     DAOOQX010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86596_length_1754_cov_3.414361"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /gene="dnaG"
                     /locus_tag="PLX53_07390"
     CDS             <1..1567
                     /gene="dnaG"
                     /locus_tag="PLX53_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOY72941.1"
                     /translation="NILHKHSDGKNIGMAYFRERGFHDNIIEKFQLGYSLDQRNAFTR
                     TALHDGYKLDYLVKTGLTIEGKDGNPFDRFAGRVMFPIHSLSGRVIGFGGRVLRTDTK
                     TAKYLNSPESEIYHKSEVLYGIFQAKKAITQEGKCFLVEGYTDVISLHQAGVENVVAS
                     SGTSLTHDQIKLIKRFTSNVTILYDGDAAGIKASLRGIDMVLEEGLNVKIVLLPDGED
                     PDSFARSHSASEVLKFIDEHETDFIRFKTLLLLDDAKNDPIKRANLIGDIVQSISVIP
                     EVIKRSVYIKECSRLMDIEENVLYTEVNRQRRKRFEQLVQRESREPKEKETPRLPSYV
                     SGVVCEEQEKEIISFLLNYGSKTLYNSQSEENKDDEINITVGQYIIDEILNDELEFQN
                     LAYKMIFDEYAQLLSQNQAVDNRHFINHYNPQISQLAVDLMSSRHKLSRIWEKHKAEV
                     TDDPDFLLHAVPKAIQVYKSKVLKIAIKNLTDELTHLSAQQKDETDEILKRLLELNKL
                     MNSMSKGLDRVVL"
BASE COUNT          531 a          391 c          424 g          408 t
ORIGIN      
        1 caacatttta cataagcatt ccgatggcaa gaatatagga atggcctact ttcgtgaacg
       61 gggattccac gacaatatta tagagaaatt tcagctgggc tatagcctcg accagcgaaa
      121 tgcctttacc agaacagccc tgcatgatgg ttacaagctg gattacctgg taaaaaccgg
      181 gctgaccata gagggcaagg atggtaaccc tttcgacagg tttgccggta gggtgatgtt
      241 tccaatccac agtctgagcg gacgggtgat tggttttggc ggacgcgttc ttagaaccga
      301 cactaaaaca gctaagtacc ttaatagccc tgaaagcgaa atataccata aaagcgaggt
      361 actctacgga atctttcagg ccaagaaggc aataacccag gagggtaagt gtttcctggt
      421 ggaaggctac accgatgtta tttcgctcca ccaggcaggt gtggagaatg tagtggccag
      481 ttcaggtaca tcgcttaccc atgaccagat taagctcatc aagcgcttca ccagtaatgt
      541 taccattctc tacgatggag atgcagctgg tattaaggct tcattaaggg gtatcgacat
      601 ggtgctggag gagggtctaa acgtaaagat tgtgctgctc cccgacggcg aggaccccga
      661 cagctttgcc cgttcgcata gcgcttccga agtgctcaag tttattgatg agcatgagac
      721 cgatttcatc cgctttaaaa cactactcct actcgatgat gctaaaaatg atccgataaa
      781 aagggcaaac ctgattggcg atatagtgca gtccatcagc gtgatacccg aggttatcaa
      841 gcgttcggtt tacattaagg aatgctcccg gctcatggat atcgaggaaa atgttctcta
      901 caccgaggtg aacaggcagc gccgcaaacg cttcgagcag ctggttcaac gcgaatcaag
      961 ggaaccaaag gaaaaggaaa caccacgttt accttcatac gtatcaggtg ttgtttgcga
     1021 ggagcaggag aaggaaatta tcagcttcct gctgaactat ggcagcaaaa cgctctacaa
     1081 cagccaatca gaggaaaata aggacgatga gattaacatt acagttggcc agtatattat
     1141 tgacgagata ctcaacgatg agctggagtt tcaaaacctg gcctacaaga tgatttttga
     1201 cgagtatgcc cagctgcttt cccagaacca ggctgttgac aaccgacact tcatcaacca
     1261 ctataaccca cagataagcc agctggcggt tgacttgatg tcgagcaggc acaagttgag
     1321 ccggatttgg gaaaaacata aggccgaagt tacagacgat cccgatttcc tgcttcatgc
     1381 cgtgccaaag gctattcagg tttacaagag caaggtgctt aagattgcca ttaaaaattt
     1441 aaccgatgag ctgacccacc tatcagccca gcaaaaagat gaaacagatg aaatactcaa
     1501 gcggctactc gagctaaaca agcttatgaa ctccatgtcg aaggggttag acagggtagt
     1561 gctttagaaa cagcagaaaa gtaaacagga aacaaggata aagagatttc aaggtaaagg
     1621 attcctgatt ctagaacctg tccggctact ggtggaactt gtccggctac tggcggattc
     1681 cagattccag atttaagatt ccagatttaa gattccagaa ttaagattcc agattcaaaa
     1741 acctgcccgc tttt
//
LOCUS       DAOOQX010000459         1752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_86792_length_1752_cov_2.293459, whole genome shotgun sequence.
ACCESSION   DAOOQX010000459 DAOOQX010000000
VERSION     DAOOQX010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86792_length_1752_cov_2.293459"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1752)
                     /locus_tag="PLX53_07395"
     CDS             complement(<1..>1752)
                     /locus_tag="PLX53_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY72942.1"
                     /translation="AQPAGFKEIFTDAEYYLLMDDYQEALPLYQKLYKLDSTNSNIHY
                     RLGQCYLNISGKKQMAIPFLEMAAQSISPKYVEGSYRETKAPLNALFYLGQAYMINME
                     LSKALNAFTQFRENLNVGDIYNLDYVNQQIKACQTAASFLEKPLNIKKERFVLLPGRN
                     VSISYPVFSGDRQSLVFTVKEKFYTALYHSVKTNGEWGTPSNITLDLGVEGEVYSTSL
                     NHDGTKLLIFINDMGSGNIYISTLEGGKWQLAKKFQGDINSRDWETFASYSADDKYIL
                     FTSNRKGGVGGLDIYISELLPGDKVSPPSNLGEIINTPYNEESPILSPDGNTLYFASQ
                     GHNSMGGYDIFYSKRIPNGQWSNPVNIGYPINTPDDEYFYFPFDSSTAVMPLASNGES
                     PYNELYQISINPAPPVRYVGLDGSIYLTDNSDIQSADVKIIITSDSILNIRDTIVPSK
                     IGKFTCTLTPGTYRVEAASNFYSMEPVHLYIPRNFNQEVYPLELKLQPKMVAEGAVIK
                     LQNILFDFDSYALTREAKFELEKVFNLMAENPSLYIEVTGHTDSKGSPIYNLKLSAKR
                     ASAVVDYLVAKGIDERRF"
BASE COUNT          438 a          348 c          453 g          513 t
ORIGIN      
        1 gaaacgtctt tcgtcaatcc cttttgcaac caggtaatcc actacggcac tggcgcgctt
       61 ggccgaaagt tttaggttgt aaatgggcga gcccttacta tcggtatgcc ctgtaacctc
      121 aatgtataat gatggatttt cggccatcag gttgaaaacc ttttcaagtt caaacttagc
      181 ttcgcgggta agggcgtagc tgtcgaagtc gaaaagaata ttctgcagct tgatgacggc
      241 gccctcggca accattttgg gttgaagttt cagctctagc ggataaacct cctggttaaa
      301 gttgcgagga atatacaggt gcaccggctc catgctgtag aagttgctgg ctgcttcaac
      361 tctgtatgtc cctggtgtta gtgtacatgt aaacttgcca attttggagg gaacaatggt
      421 atctcttatg ttcagtatac tgtcggaggt aatgataatt ttaacatcag ccgattggat
      481 gtcggagttg tcggtaaggt agatggagcc gtcgagtcca acgtacctta ccggtggggc
      541 aggatttatg gagatttggt aaagttcgtt gtatggcgat tcaccgttgg aggctaaggg
      601 cataacggcg gtgctggaat caaaaggaaa gtaaaaatac tcatcgtcgg gagtgtttat
      661 ggggtatccg atatttacag gatttgacca ttgcccgttg ggaatacgtt tggagtagaa
      721 gatatcgtat ccgcccatgc tgttatgtcc ctgtgatgcg aagtaaaggg tattaccatc
      781 ggggcttaaa atgggcgatt cctcgttgta gggtgtattt atgatttcac caaggttgga
      841 cggaggcgaa actttatcac caggaagtaa ctcggaaatg tatatatcaa gccctccaac
      901 gccaccttta cggttcgaag taaaaagaat gtacttatca tcggccgagt aggaggcaaa
      961 ggtttcccag tctctagagt ttatatcacc ctgaaatttc tttgcaagtt gccactttcc
     1021 accttcaagg gtagatatgt agatattccc tgaacccatg tcattaataa aaataaggag
     1081 tttggtgcca tcatggttaa gggatgtgga gtaaacctca ccttctacgc ccaagtcaag
     1141 ggtgatgttg ctgggtgttc cccattcacc attggttttc acgctatggt atagagcggt
     1201 gtagaatttt tcctttacag taaatactaa actttgccta tcgcccgaga aaaccggata
     1261 gctaatgcta acatttctgc ctggaagcag gacaaaccgc tcctttttta tgttgagcgg
     1321 tttttcgaga aaagaggcgg cggtttggca ggccttaatc tgttggttta catagtcgag
     1381 gttgtatata tcgccaacgt tcaggttctc ccggaattgg gtaaacgcat tgagggcttt
     1441 ggaaagctcc atgttgatca tgtaggcctg cccaaggtaa aagagggcat tcaacggagc
     1501 ttttgtttca cggtatgacc cttctacgta ctttgggcta atgctttgag cggccatttc
     1561 taggaagggt atggccattt gttttttccc ggaaatattc aggtagcact ggccaagccg
     1621 gtaatggata ttgctgttgg tagaatccag cttgtaaagt ttctggtaaa ggggtagcgc
     1681 ttcctggtaa tcgtccatca gaaggtagta ttcggcatcg gtaaatattt ccttaaatcc
     1741 tgcgggttgg gc
//
LOCUS       DAOOQX010000460         1751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_86841_length_1751_cov_3.305425, whole genome shotgun sequence.
ACCESSION   DAOOQX010000460 DAOOQX010000000
VERSION     DAOOQX010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86841_length_1751_cov_3.305425"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..892)
                     /locus_tag="PLX53_07400"
     CDS             complement(113..892)
                     /locus_tag="PLX53_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72943.1"
                     /translation="MLIFFVVFCKVSFSQEKLLPFDTLSIVHFRVVNANTREPVERAY
                     ILNLTYNWGSISDLLGYSYLPIHFGDKLKISAIGYYTEELICVGQFKADSAFYEIKLK
                     PRVYELNEVKITKFSTYERFLRGVINLNIPKTRDDKQLEVIEKYLFGVVKKMDLKNTP
                     TMASGIAFGKDWYAKQNEKLAEQLEREKTRRVIDEKFNPGLVQKHTGLSDKELYEFIG
                     YLDFSDEFLLQATDYEIHQKIMEKYNVFMAGKSNELKNQEK"
     gene            complement(1132..>1751)
                     /locus_tag="PLX53_07405"
     CDS             complement(1132..>1751)
                     /locus_tag="PLX53_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOY72944.1"
                     /translation="PYRFIRPSVLSPILWVVRGYAVFDNMSMPIVARNGGEPNDTFIE
                     QLVENAKAAVDTLVGMGVANPQKVAIGGHSYGAFMTANLLAHSDLFAAGIARSGAYNR
                     TLTPFGFQNEERTYWQAPEVYNRMSPFMHADKINEPLLLIHGENDNNSGTFPLQSERL
                     FAAIKGHGGTARLVMLPYESHGYRARQSVFHTIWEMDCWLEKYLK"
BASE COUNT          485 a          416 c          340 g          510 t
ORIGIN      
        1 tttacactcg ccgacgaatg aaccggagta tgcttcacct tatcctttat cagctcatag
       61 gccgaaacta cgtcggaagc ttctggttga tagcagcttg aattctcctc actcattttt
      121 cttgattttt taactcgttt gattttcccg ccataaagac gttatacttt tccattattt
      181 tttgatgtat ctcgtaatca gtagcctgca ggaggaactc atcgctgaag tctaaataac
      241 cgataaactc gtaaagttcc ttatcactca ggcctgtatg cttctggaca agcccgggat
      301 taaatttttc atctattacc ctccgggttt tctccctctc cagctgttct gccaacttct
      361 cattctgctt ggcataccaa tccttaccaa aagcaatccc cgaagccatg gtgggtgtat
      421 tttttaaatc catcttcttt acaacgccaa acaggtattt ttcaatcacc tctagctgct
      481 tatcatccct ggtcttagga atatttaggt taatcacacc tctaagaaac ctttcgtagg
      541 tcgaaaactt ggtgatttta acctcgttca actcatacac cctgggtttt agctttatct
      601 cataaaatgc agaatcggct ttaaattggc caacgcaaat caactcttca gtatagtatc
      661 caattgcaga aattttaagc ttatcgccaa agtgtatcgg caaatagctg taacccagca
      721 aatcggaaat acttccccag ttgtaggtta aattcagtat gtaggctctt tcaacaggtt
      781 cccgggtgtt tgcattgaca accctgaagt gaacaatgct aagcgtatca aagggtagta
      841 gcttttcctg gctgaatgat actttgcaga aaaccacaaa gaaaataagc acccatattt
      901 tacgaaaacc aaccaagcta tcatttttcc caaaaatagc gtaatattta attattaaaa
      961 gtgatgcggc agcttcggtt aacttttttt aacgccatca atttactggg atgaatttcc
     1021 agaaaatcca aacaggtaag aaaaattgaa aagtgctaag aaaactccta aacataaacc
     1081 gtgaatcctt tatccatgaa ttatatcatc aaatctatta acaggctcat atcatttcag
     1141 gtatttttcg agccagcaat ccatttccca aatggtgtga aaaaccgact gcctagctct
     1201 gtagccatga ctttcgtatg gaagcattac cagcctggct gttccaccgt gtccttttat
     1261 agcggcaaac agccgttcgc tctgtagcgg gaatgttccg ctattattat cgttttcgcc
     1321 atgaatgagc aaaaggggct cgttaatttt atcggcgtgc atgaagggag acatgcgatt
     1381 gtaaacctca ggggcttgcc agtaggttcg ctcctcgttc tgaaagccaa atggggtaag
     1441 ggttctgttg taagccccgc tgcgggcaat tccggctgca aaaagatcgg agtgggcaag
     1501 caggttggca gtcataaaag caccgtagga atggccgcca attgccacct tttgggggtt
     1561 agccactccc atgcccacca gcgtatccac tgctgccttc gcgttctcta ccagctgttc
     1621 aatgaaggta tcgttgggtt caccgccatt tctggctact attggcattg acatgttatc
     1681 gaataccgca tacccacgca caacccaaag tataggcgac aaaacagacg gccggataaa
     1741 ccgatagggc g
//
LOCUS       DAOOQX010000461         1747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_87116_length_1747_cov_3.560875, whole genome shotgun sequence.
ACCESSION   DAOOQX010000461 DAOOQX010000000
VERSION     DAOOQX010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87116_length_1747_cov_3.560875"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLX53_07410"
     CDS             <1..345
                     /locus_tag="PLX53_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72945.1"
                     /translation="RMMKFDNLSPIENRTLDVVKKLVNLRRNTMALNYGNFETLLADS
                     TQYAYARTYLQSSAIVVFNKSDEDKSIIFKLPDYVVGRNFTSKFGSNFSIQNQIVKVD
                     LKPNSFEILISK"
     gene            complement(563..635)
                     /locus_tag="PLX53_07415"
     tRNA            complement(563..635)
                     /locus_tag="PLX53_07415"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(600..602),aa:Lys,seq:ttt)
     gene            927..1733
                     /locus_tag="PLX53_07420"
     CDS             927..1733
                     /locus_tag="PLX53_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY72946.1"
                     /translation="MKGKIAIITGASSGIGKACAYEFASRGAKLVLAARNLEVLNSIV
                     QDLLLNGHEAIAVRTDVTVEDDCKNLVDEALKRYGKIDYLLCNAGISMRALFKDVDLK
                     VIRQLMEVNFFGAVYCTKYALPHLLASRGSVIGVSSIAGFHGLPGRSGYSASKFALQG
                     FLETLRIENLKNGLHVMVAAPGFTATNVRVSALTADGSPQGVSPRDEKHMMTPQKVAR
                     TIAHGILRKRRNIIMTTEGKLSVLAQRVFPKIIDRILYMFMAREPNSPFK"
BASE COUNT          528 a          340 c          347 g          532 t
ORIGIN      
        1 aggatgatga aatttgataa cctttcacca atagagaaca ggacacttga tgttgtaaaa
       61 aaactggtga accttagaag aaacaccatg gcccttaact atggtaactt tgaaaccctt
      121 ttagccgact caacccagta tgcttatgcc agaacatacc tgcaaagttc tgcaatagtt
      181 gtctttaata agagtgatga ggataaatct ataattttta aattgcctga ttatgtggtg
      241 ggtagaaact ttacctcaaa gtttggctca aatttttcca ttcaaaacca aattgttaaa
      301 gtagatttaa agccaaactc atttgagata ctcatatcca agtagatacg ttttttatta
      361 ctcctaataa gagccctaca tcgtagggct tttttgttga gcattagctt attaaggcaa
      421 tggaaacagg aatccggctg taggaagtaa aaatttgaaa tctacgctct atttaatttg
      481 caggctgtga attttggaat ctgtacataa aaaaaccatc cactatgatt atggatggta
      541 atgttgaata gcctataact tgtgatccag ctgggattcg aacccaggac cccatcctta
      601 aaagggatgt gctctaccag ctgagctact gaatccttct atcaaatctt attataactt
      661 atgttacata agtttgccta aaggcctgca aatttaatta attttttacc taaacatcca
      721 atttcttacg ttgaattatt tacaaaccaa ataaaaaatt tatacaacat tgaaaatgag
      781 cacatcgcat tataatcttg tgcaagatat ctttaaaagt ttcgcaacac tttctaattc
      841 tccatgtttt acttattttt agtgcggaga atattttttt aaactttagt atattatagc
      901 taatttttat gtcaactcaa actagcatga aagggaagat tgccattata acaggcgctt
      961 cttcaggtat tggaaaggct tgcgcatacg aatttgcatc aagaggtgca aagctggttt
     1021 tagctgcccg taatcttgaa gtgctgaata gcattgttca ggatttgttg cttaacgggc
     1081 atgaagccat tgcagttaga accgacgtaa cagtcgagga tgattgtaag aatttagtag
     1141 atgaggctct gaaacgatac ggaaaaatcg attacctgct ttgcaatgcc ggcatttcga
     1201 tgcgtgcact tttcaaggat gttgatttaa aggtaattag acagctaatg gaggttaact
     1261 tttttggcgc tgtttactgc accaagtatg ccttacccca ccttctggca agtcgcggct
     1321 ctgttattgg agttagctca attgctggtt ttcatgggct gccgggtcgc tcaggatatt
     1381 cagcttctaa atttgccctt cagggttttc tggaaacact ccgtatcgag aatcttaaaa
     1441 atggcctcca tgtgatggta gcagccccag gatttactgc aaccaacgtc agagtttccg
     1501 ctctcacggc agatggaagc ccgcagggcg tgtctccccg cgacgaaaag cacatgatga
     1561 ctccccagaa ggtggccaga acaattgctc atgggatact gaggaaaagg cgaaatatta
     1621 tcatgacaac cgaaggtaag ctatctgtgc tggcgcaacg ggtcttccct aagattattg
     1681 accgaatact atacatgttc atggcccgag aaccaaattc tccgtttaaa tagccgtttt
     1741 acctgca
//
LOCUS       DAOOQX010000462         1743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_87447_length_1743_cov_2.699052, whole genome shotgun sequence.
ACCESSION   DAOOQX010000462 DAOOQX010000000
VERSION     DAOOQX010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87447_length_1743_cov_2.699052"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLX53_07425"
     CDS             complement(<1..376)
                     /locus_tag="PLX53_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOY72947.1"
                     /translation="MSSTAIIILIVCYFTALFIISYFTSRRSDNQSFFIGNRRSPWYL
                     VAIAMIGTSISGVTFISVPGWVGTSQFSYLQMVLGYLLGYFVIANVLLPLYYRLQLTS
                     IYTYLDKRFGFYSYKTGAFVEVG"
     gene            785..1411
                     /gene="recR"
                     /locus_tag="PLX53_07430"
     CDS             785..1411
                     /gene="recR"
                     /locus_tag="PLX53_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013265871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOY72948.1"
                     /translation="MNVQSYPSKLLETAVEEFSKFPGIGRRSALRLVLHLLRQDIEDV
                     DSFTRAITDLGHNVKYCRVCNNISDTDTCGICSSPHRDAGLICVVESIKEVMMIESTH
                     QFNGLYHVLGGVISPMEGIGPADLSIEKLVDRVNQGGVREVIMALSATTEGDTTNFYI
                     FKKLKDKPVLFSTLARGVAFGDELEFTDEVTLGRAILNRVKFDASIPD"
     gene            1506..>1743
                     /locus_tag="PLX53_07435"
     CDS             1506..>1743
                     /locus_tag="PLX53_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72949.1"
                     /translation="MGNRFTRILLLLLISSHSIAQTSFNELVSSANLNLKERNYKAAA
                     DQFKKALEIQPDDTFALQGVIKCYTLSEDYKNAQR"
BASE COUNT          494 a          351 c          437 g          461 t
ORIGIN      
        1 cacctacctc gacaaacgct ccggttttgt aggagtaaaa cccaaagcgt ttgtcgaggt
       61 aggtgtatat tgatgttagc tgaagccggt agtataatgg cagcagcacg ttggcaataa
      121 caaagtagcc gaggaggtag cccagcacca tttgcaggta ggagaactgg ctggttccaa
      181 cccatccggg aaccgaaata aaggtaactc ccgaaatgga tgttcctatc atggcgatag
      241 ccaccaggta ccaaggcgag cgcctgttac caataaaaaa cgactggtta tcggaacgcc
      301 tggaggtgaa gtaggaaata ataaagagtg ctgtgaagta gcagacaatc agaatgataa
      361 ttgcagttga cgacataaac tcctggtttt aggcgctgca agtttaatca atttaagcca
      421 aaatatgcta gggttcggga cagcggtttt tgattaggtg aatggggatt cgttttatac
      481 tgctcatccg ccaagcataa attccaaaat tttgttttat gcttgggtaa ttctttcagg
      541 taatctcctg cttgaaggga taaagctggt atttcatctt attttccttt aatacgggga
      601 ggaatttgcc atttaattct aaattcatgt agatttgtgg aaactttaaa ttttatcgaa
      661 cccaactttc cgattgtgag tggctgtgtc ccaagttgaa catgggaata gcctaaggga
      721 gtacgaagtg ccaaaaaaat tgatggttgc aaggctgttg ataaaaatga ataaagaata
      781 gagtatgaat gtccagtcat atccctcaaa gttgctggaa actgctgttg aagaattttc
      841 aaagttcccc ggaataggca ggcgttcggc cctgaggttg gtgctccatc tgctacgtca
      901 ggacatagag gatgttgata gcttcaccag ggccataacc gatctgggtc ataatgtgaa
      961 gtactgcagg gtgtgcaaca atatatccga tacggatact tgcggcattt gctcatcgcc
     1021 ccatcgcgat gcggggctta tttgcgtggt ggagagcatt aaggaggtca tgatgattga
     1081 gagcacccac cagttcaacg ggttatacca tgttcttgga ggcgtgataa gccccatgga
     1141 ggggattggc cctgccgacc tgagcatcga aaagctggtt gaccgggtaa accagggagg
     1201 agtaagagag gttataatgg ctcttagcgc taccaccgag ggcgatacca ctaattttta
     1261 catctttaaa aagttgaagg acaagcctgt tttattctcc acgttggcac gaggggtggc
     1321 ttttggtgac gagttggagt ttaccgacga ggtaaccctg ggcagggcaa tcctcaacag
     1381 ggtgaagttc gatgcttcta tcccggatta gctagaggaa aaatttgttt tggcatcacg
     1441 aattttggtg taactttatg attggtaatt tggtaaaaca tatcatccag caaatccctt
     1501 tagctatggg aaatcgattt acacgcattc tcctgctact ccttatttct agccacagta
     1561 ttgcccaaac cagcttcaac gagctggtaa gctcggcaaa cctgaacttg aaggaaagaa
     1621 actataaggc tgccgccgat caattcaaaa aggcccttga gattcaacct gacgatacct
     1681 ttgccctaca gggggtaatc aaatgctaca ctctttcgga agattacaag aatgcccagc
     1741 ggt
//
LOCUS       DAOOQX010000463         1739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_87796_length_1739_cov_2.988124, whole genome shotgun sequence.
ACCESSION   DAOOQX010000463 DAOOQX010000000
VERSION     DAOOQX010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87796_length_1739_cov_2.988124"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLX53_07440"
     CDS             <1..219
                     /locus_tag="PLX53_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOY72950.1"
                     /translation="YADAHDVKHEYGFDMATAPSGRYDAIILAVGHSQYAGLKEEWFK
                     RLAAEKCVFVDVKGIFRSKINNLTYWSL"
     gene            411..>1739
                     /gene="thrS"
                     /locus_tag="PLX53_07445"
     CDS             411..>1739
                     /gene="thrS"
                     /locus_tag="PLX53_07445"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOY72951.1"
                     /translation="MVKITFPDGSIREYPLGVTGIEIARSISERLAAEVLSITVNGEV
                     WDLTRGITTDATVKLHKWDDEEGKHTFWHTSAHLMAQALEALYPGIKLGIGPSIDNGF
                     YYDIDFDSYTISDDDFPKIEQKMAELAHQKLDVVRKDVSKDEALATYKAKGDPYKVEL
                     ISDLQDGTITFYTQGDFTDLCRGPHLPNTGYIKAIKILSIAGAYWRGNEKNKMLTRIY
                     GITFPQKKLLDEYLVMLEEAKKRDHRKLGKEMELFTFSQHVGQGLPLWLPRGAQLRFQ
                     LEMFLRKVQQKYGYEQVITPHIGQKELYEISGHWAKYGKDSFRPITTPQEGEEFLLKP
                     MNCPHHCEIYKSKPRSYKDLPVRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCR
                     PDQLKQEFVKVIDIVLYIFKTLNFKEYTAQVSLRDPKDREKYIGTDENWEK"
BASE COUNT          495 a          383 c          414 g          447 t
ORIGIN      
        1 tatgccgatg cccatgacgt aaagcacgag tatggttttg acatggcaac tgctccatcg
       61 ggcaggtatg acgcaatcat tctagccgtt gggcatagcc agtatgctgg actcaaggag
      121 gaatggttta agcgccttgc tgccgaaaag tgcgtttttg tagatgtaaa gggtattttt
      181 aggagcaaaa ttaacaacct cacctactgg agcctttagg tggtttgatg gatagtttgg
      241 ctggtatttc tactaattcg tactaaacat ggcataataa tctggccatg tttgccttaa
      301 cgggctaatg ccataaacca gccccattcg tgaatgcctt gcaaatttgg agttaatagt
      361 attactttgc aatctttaac ttttattaca agtatatcaa gtttataatg atggtaaaga
      421 ttacttttcc tgatggtagc atacgggagt atccccttgg ggtaaccggg attgaaattg
      481 cccgaagcat ttcggaacga ctggctgccg aggttctatc aatcactgtt aatggtgagg
      541 tttgggattt gactcgtgga attactaccg atgccaccgt aaagctgcac aagtgggatg
      601 acgaagaggg aaagcatacc ttttggcaca catccgctca ccttatggcg caggcactcg
      661 aggcccttta cccgggcatt aagttgggaa ttggccctag cattgacaac ggtttttact
      721 acgatataga ttttgatagc tacaccattt ctgatgacga tttcccaaaa attgagcaga
      781 aaatggccga gctggcccat caaaaacttg atgttgttag gaaggatgtt tcaaaagacg
      841 aggcactagc cacatacaag gctaaaggcg acccatacaa agttgagctt atcagcgacc
      901 tacaggacgg caccattacc ttctacaccc agggcgattt caccgacctt tgccgtgggc
      961 cacaccttcc aaacacaggc tacattaagg ctattaaaat tttaagtatt gcaggagcat
     1021 actggcgtgg gaacgagaaa aacaaaatgc taacccgtat ctatggcata acctttccac
     1081 agaaaaaatt gctcgacgag tacctggtaa tgctggaaga ggccaaaaag cgcgaccatc
     1141 gtaagctggg gaaagaaatg gagctattta ccttttcgca gcatgttggt caggggttac
     1201 ccctatggct gccccgtggc gcccagcttc gctttcaact cgagatgttc cttcgaaaag
     1261 tacagcagaa gtacggctac gaacaggtaa taactcctca catagggcaa aaggaacttt
     1321 acgagatttc tgggcactgg gcaaaatacg gcaaggatag ctttagaccc attactaccc
     1381 ctcaggaggg ggaggagttt ctccttaaac ccatgaattg cccacatcac tgtgaaattt
     1441 ataaatctaa accccgttcc tataaggact taccggttag gttagccgaa tttgggacgg
     1501 tttaccgcta cgagcaaagc ggcgagcttc acgggttaac acgcgttagg gggtttactc
     1561 aggacgatgc ccatatcttc tgtcgccccg accagctcaa gcaggagttt gtcaaggtga
     1621 ttgatattgt tttgtatatt tttaaaaccc tcaattttaa ggagtacacc gcacaggttt
     1681 cactccgtga cccaaaggat agggaaaaat acattggaac ggacgagaac tgggaaaaa
//
LOCUS       DAOOQX010000464         1738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_87876_length_1738_cov_3.033868, whole genome shotgun sequence.
ACCESSION   DAOOQX010000464 DAOOQX010000000
VERSION     DAOOQX010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87876_length_1738_cov_3.033868"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLX53_07450"
     CDS             complement(<1..217)
                     /locus_tag="PLX53_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOY72952.1"
                     /translation="MKDRQLDIAVISDLHLATYACKPKKILKYLKSIKPQMLVLNGDI
                     IDSWRFSRNYFPKNHMRVVRQIIKMMEK"
     gene            457..>1738
                     /locus_tag="PLX53_07455"
     CDS             457..>1738
                     /locus_tag="PLX53_07455"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HOY72953.1"
                     /translation="MKQPKKKREFPHTYVIIFTLIVVAALLTWFIPGGEFTREVKSIN
                     GIDREVIVPGSFHQVDNSPQTWQVFTSIFQGMRRTCDIIFYILIIGGSFWMLNESKAL
                     DVAILSFLNFTRRIERFKPLKAIGVNNLVIALIMICFSFFGAVIGMSEETIAFVVIFV
                     PLAISMGYDSIVGICLCFLGAGLGFASALLNPFTIGIAQGLSGIPLFSGIEYRFIIWL
                     VINTIGISYVLWYANRIRKNPKLSPVYEIDEHWRHKASEEGAIVKTKAGKIAWVVFAV
                     VAIVLAIFAYLYPTSQLVVGQNVISVPAIPIATVVWVILGLLALRHSVQLFIVNILMF
                     TILFLIVGVMGYGWYIKEIASLFLVMGILASAAFGKNANQLTRSFIDGARDIMSAALV
                     VGLASGIVVVLEQGKVIDSLLNYSAWRIVSPEASL"
BASE COUNT          427 a          400 c          436 g          475 t
ORIGIN      
        1 ccttctccat catcttgata atctgcctta caaccctcat gtggttcttg ggaaagtaat
       61 tcctgctgaa acgccacgaa tcaataatat caccgttgag gacaagcatc tgcggcttaa
      121 tagatttaag gtacttcaga attttttttg gtttgcaggc ataggtagcc aggtgcagat
      181 cggaaatcac ggctatgtcc aactgtctat ccttcattta gctacaggta taaattttat
      241 gcaaaggagc agtttagttg taaaatacat gttactaaaa cgttaggatt tgaataatcc
      301 tttgttacca ctttacaccg cacaattact atagggtagc attaaccatg cagggcgcat
      361 tttgccatta taaaaagaat aaaaatggat ggttgccacc accgacgatg gatgaatatg
      421 aaaaaaaact attttagcca ttcaaaaata ggcgcaatga aacaacctaa gaagaaacgc
      481 gagtttccgc atacctacgt catcatcttc acgcttatag ttgtggcagc ccttctcacc
      541 tggtttatcc ccggagggga gttcacccgg gaggtgaaga gtatcaacgg gattgatcgc
      601 gaagtgattg ttcccggctc ctttcaccag gttgacaaca gcccgcaaac atggcaggtg
      661 ttcacctcca tttttcaggg catgaggcgt acctgcgaca ttatcttcta cattctgata
      721 atcggcggtt cgttttggat gctaaacgag agtaaagcgt tggatgtggc catcctgtcg
      781 tttctgaact ttacccggcg aatagagagg ttcaagccac tgaaggcaat cggggtgaat
      841 aacctggtga tagcgctcat catgatctgc ttcagctttt ttggggcggt aatagggatg
      901 agcgaggaga ccatcgcctt tgtggtaatt tttgtgccgc tggccataag catgggctac
      961 gactccattg ttggaatatg cctgtgcttt ctgggtgcgg gtctgggttt tgccagcgca
     1021 ctgctaaacc cctttaccat aggaattgcc cagggacttt cgggaatacc gctgttttcg
     1081 gggattgagt accgattcat aatctggctg gtgatcaaca ccataggtat tagctacgtg
     1141 ctatggtatg ccaaccgcat caggaagaac cccaagctat ctccagttta cgagattgac
     1201 gagcactggc gccacaaggc ttcagaagag ggggcaatcg ttaaaaccaa agccggcaaa
     1261 atcgcatggg ttgtctttgc cgtggtagcc attgtgctgg ccattttcgc ctacctctac
     1321 cccaccagcc agctggtagt cggccaaaat gtcatttcgg ttccggctat tcccatagcc
     1381 accgtagtgt gggtcatact agggcttctg gcattaaggc actccgtgca gctgttcatt
     1441 gtaaacatcc tcatgttcac catcctgttc ctcattgttg gcgttatggg ctatggatgg
     1501 tacatcaagg aaatagcatc gctattcctg gtcatgggta ttctggcctc tgctgcattt
     1561 ggtaagaacg ccaaccagct tacacgctcg ttcattgatg gggcaaggga tatcatgtcg
     1621 gctgcgctgg ttgttgggct ggccagcggg attgtggtgg tgctggagca aggaaaggtt
     1681 atagattcgc tgctgaacta ctcggcctgg cggatcgtca gcccagaggc gagcctgg
//
LOCUS       DAOOQX010000465         1735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_88147_length_1735_cov_2.333333, whole genome shotgun sequence.
ACCESSION   DAOOQX010000465 DAOOQX010000000
VERSION     DAOOQX010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88147_length_1735_cov_2.333333"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..253)
                     /locus_tag="PLX53_07460"
     tRNA            complement(181..253)
                     /locus_tag="PLX53_07460"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(218..220),aa:Gln,seq:ctg)
     gene            complement(359..>1735)
                     /locus_tag="PLX53_07465"
     CDS             complement(359..>1735)
                     /locus_tag="PLX53_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOY72954.1"
                     /translation="ASDYFEQLYEWAEVLIKKGLAYVDDQTQEEIRINRGTVNQPGKP
                     SPWRNRSVDENLDLFRRMRAGEFGDGERVLRAKIDMAHPNMLMRDPIMYRIIHAEHHR
                     TGSKWCIYPMYDYAHGQSDSIEKITHSICTLEFDVHRVLYEWFIEKLEIFPSRQYEFA
                     RLNLSYTVMSKRKLLELVREKIVMGWDDPRMPTICGLRRRGYTPESIRYFADKVGVAK
                     RDNVIDLSLLEFCLREDLNKRAERRMAVLSPLRVLITNYPEGQVEELDAINNPEDDAA
                     GTRSVPFSREIYIEQDDFMETPPKKFFRLAPGQEVRLRYAYFIRCNEVIKDEQGNIIQ
                     LNCTYDPASRGGNSPDGRKVQGTIHWVSAQHAIKADVRLFDRLFSKPDPNDVPEGVDW
                     KSTINSDSLRVVEAYIEPSLKNVKPLDKFQFERLGYFSVDYDSTSQKLVFNRTVTLKD
                     TWAKIAGK"
BASE COUNT          414 a          456 c          427 g          438 t
ORIGIN      
        1 ctccaaacat actgagctgg caggcaagtt aatagaacta ctcttagcca tgaggcttga
       61 ggctaaaagg cagaaggact ttgcaacatc cgatagaatt cgcagcgagt tggaacagct
      121 gggagttgtt gtgcgtgacc gtaaagacgg gttcgactgg gagattagct agaaaaaatg
      181 ttgtcctgcc aggggtcgaa cctggactct tctgatccag agtcagatgt gttgccaatt
      241 acaccacagg acaattttta cggtgcaaat atagctaatt agccttttac tggtaattct
      301 accgggaaaa tttttattcc ccatgaccta ccaagccgca ataggctttt cgcctttgct
      361 acttccccgc aattttagcc catgtatcct tcagggtaac ggtgcggttg aaaaccagct
      421 tctgggatgt agagtcgtaa tccacgctaa agtagccaag cctttcaaac tggaatttat
      481 cgagaggttt aacattcttc aacgagggtt caatgtaggc ttcaaccacc ctgagcgagt
      541 cggaattaat ggttgatttc cagtcaaccc cctcgggaac atcgttgggg tcgggcttgg
      601 aaaaaagcct gtcgaacaag cgtacatcag ctttaatggc atgttgagcc gatacccagt
      661 ggatggttcc ctgaaccttc ctaccatcgg gtgaatttcc acctcgggag gccgggtcgt
      721 aggtgcagtt gagctgaata atgttaccct gttcgtcctt aatcacctcg ttacacctga
      781 taaagtaggc atatcgaagc cgtacctcct gccctggcgc caaccggaag aactttttcg
      841 gcggagtctc cataaagtca tcctgctcaa tgtagatctc cctactgaat ggcacactcc
      901 gagtaccggc cgcatcatcc tcggggttgt taatagcatc aagctcttcc acctgcccct
      961 cgggatagtt ggtaatcagc acccgcagcg ggctaagcac cgccatccgt cgttcggcac
     1021 gtttgttcaa atcctcccta aggcaaaact ccagaagtga aaggtctatc acgttatcgc
     1081 gcttggccac ccccaccttg tcggcaaagt agcggatgga ctccggagtg tagcccctgc
     1141 gtcgtagccc acaaatggtg ggcattcttg gatcgtccca ccccataaca atcttctccc
     1201 taaccagctc cagcagctta cgcttgctca tcaccgtgta ggagaggttg agccgggcaa
     1261 actcgtactg cctcgagggg aaaatttcaa gcttttcaat gaaccactcg tacagcacgc
     1321 ggtgaacgtc aaactcaagt gtgcagatgg agtgggtaat cttctcgatg gagtcggatt
     1381 gcccgtgggc gtaatcgtac attggataaa tgcaccactt gctacctgtt cgatggtgct
     1441 cggcatgaat aatcctgtac atgattgggt cgcgcatgag catgttgggg tgcgccatgt
     1501 caatttttgc acggagcacc ctttcgccat cgccaaactc cccggcccgc atccgacgga
     1561 acaggtcaag attttcgtca accgatcggt tacgccaggg gctaggttta ccgggctggt
     1621 taactgtgcc gcggtttatc cttatttcct cctgggtttg atcgtcaaca taggctagcc
     1681 cctttttaat gagaacctct gcccactcgt agagctgttc aaagtagtcg gaagc
//
LOCUS       DAOOQX010000466         1732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_88358_length_1732_cov_3.259988, whole genome shotgun sequence.
ACCESSION   DAOOQX010000466 DAOOQX010000000
VERSION     DAOOQX010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88358_length_1732_cov_3.259988"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLX53_07470"
     CDS             <1..352
                     /locus_tag="PLX53_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOY72955.1"
                     /translation="VEKFHIPMLWIGGALSVNPTRIDETVSQVDLASTLLNQLNLSDS
                     AFTFSKNILSSHFTPFAYYAFNDGFGFKNNTDTVIYDHAGKKYIKMSRANPKQAAQEA
                     WAFFSTYQDTFLGY"
     gene            complement(408..1493)
                     /locus_tag="PLX53_07475"
     CDS             complement(408..1493)
                     /locus_tag="PLX53_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72956.1"
                     /translation="MKAFLRISVTLFLLLSSLSGYSASLELSKQYDREFKVSPASKLY
                     VENKYGQISIENWDKNAIAIHVDVKVENANAERAKSMLDAINVEFSETENLVSAITRF
                     NEDIRKSSPRLFSSFSNTELSINYKIFMPKNIEVELLNKYGNIFVNELNARITVDLMY
                     GNFKANKLGLGDSQPLSSITVGYGDVAISEANWLKVEMKYSNLNIEAAQAMVVLSKYS
                     KVYLGRVNSLVLESKYDRYEVGSVMNLEGESGYTDFDISNLGKKFNLTTRYGNVKIID
                     VSDSFQSIRFEGAYTGLKAGINPNVSYRIDAEAAYGSINFSADKSSVNRIEDNTTIQV
                     SGLVGTNSRTNAEVYIRIKYGNANLNK"
     gene            complement(1541..>1732)
                     /locus_tag="PLX53_07480"
     CDS             complement(1541..>1732)
                     /locus_tag="PLX53_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72957.1"
                     /translation="STDPSVKPEAKQLIEEFDREGQRLKAELPKNPRSDMVLGAMYEN
                     YQSRLEVLQKIEGTLDKGK"
BASE COUNT          469 a          395 c          322 g          546 t
ORIGIN      
        1 cgttgaaaaa ttccatattc ccatgctgtg gataggaggc gcgcttagcg ttaatccaac
       61 acgaattgat gaaactgttt cgcaggttga ccttgcctca acgctcctca accagctcaa
      121 cctcagcgat tcagctttta ctttttccaa gaatatcctt agcagccact ttactccttt
      181 tgcatactat gcctttaatg acggctttgg ttttaagaat aataccgata cggtcatcta
      241 tgaccatgca ggtaagaagt acatcaagat gagccgagca aaccccaaac aggctgctca
      301 ggaagcatgg gcattttttt ccacttacca agacaccttt ttaggatact agtgtgcttg
      361 tcgccttatc aaattaaaac ggggaggttc cttgcctccc cattttttta cttgttaagg
      421 tttgcgttgc catacttaat cctgatgtac acttcagcat ttgttctgct atttgttcct
      481 accaggcccg atacctgaat tgtggtgttg tcttcaattc ggttgacaga tgatttatcg
      541 gcgctgaaat ttatactacc ataggctgct tcagcgtcaa ttctgtagga aacattaggg
      601 ttgataccag ccttcaagcc ggtgtaggcc ccctcaaacc gaatgctctg aaagctatca
      661 gacacatcta ttattttaac gtttccatac ctggtggtta ggttaaactt tttgcccaag
      721 ttggaaatat cgaaatcggt ataaccagac tctccttcca aattcataac cgaaccaacc
      781 tcatacctat catatttaga ttcaagaaca agcgagttta cccttcccaa gtaaaccttg
      841 gagtactttg aaagaactac cattgcctgt gctgcttcaa tgttaaggtt gctgtacttc
      901 atttcaacct ttaaccagtt tgcctcggaa atggcaacat caccatatcc aacggtaatg
      961 gagctaagag gttgcgaatc gcccagccct agcttgttag ccttaaaatt gccgtacatc
     1021 aaatcaactg taatcctggc atttagctcg ttcacaaata tattcccata cttattcaac
     1081 agctccacct cgatattttt cggcataaat atcttatagt taatgctaag ctcggtgtta
     1141 gagaaagatg agaacagtct aggagatgac tttcgaatat cctcattaaa ccgggtaata
     1201 gccgaaacca ggttttccgt ttcagaaaac tcaacattaa tagcatcaag catagattta
     1261 gccctttcgg cgtttgcgtt ttcaactttc acatctacat gaatggctat agcgttttta
     1321 tcccaattct caatgctaat ttggccatac ttgttttcaa cgtaaagttt agatgcaggc
     1381 gaaaccttaa actccctgtc gtattgttta gaaagctcga gggatgcgga atatccgctt
     1441 aagctgctca gtaataagaa tagagttaca gaaattctga gaaatgcttt catggttttt
     1501 tatctattaa gttaaattat taacatggca aaaagtgctg ctacttcccc ttatcaagtg
     1561 ttccttcaat tttttgtaga acttccagcc ttgactggta gttttcgtac attgctccaa
     1621 gcaccatgtc gctgcgggga ttctttggta gttctgcttt aaggcgttga ccctccctat
     1681 caaactcctc aatgagctgt ttggcttcag gttttacgga aggatcggtt ga
//
LOCUS       DAOOQX010000467         1732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_88385_length_1732_cov_2.341682, whole genome shotgun sequence.
ACCESSION   DAOOQX010000467 DAOOQX010000000
VERSION     DAOOQX010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88385_length_1732_cov_2.341682"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLX53_07485"
     CDS             complement(<1..483)
                     /locus_tag="PLX53_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HOY72958.1"
                     /translation="MKQVVIISRYLLAVVFIFSGFVKGIDPMGSAYKFHDYFMAFHMN
                     FMQPLSLAFAFILCAAELLIGLMLLYGVNLRIAAWGVFIFMLAFTPLTLILAIFNPVS
                     DCGCFGDALHLTNWQTFFKNIVLLSAAYTFFRYRASLPPSFGEKKQSAMFAVLCVVAF
                     L"
     gene            complement(509..1201)
                     /gene="tmk"
                     /locus_tag="PLX53_07490"
     CDS             complement(509..1201)
                     /gene="tmk"
                     /locus_tag="PLX53_07490"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOY72959.1"
                     /translation="MALIVIEGLDGAGKSTQINLLGSFLTQKGVASHFLHFPRVDAPY
                     FGELIARFLRGDLGRIDEVDPYVVAMLYALDRMDASGLIKSWLKTDEVVLLDRYVYSN
                     IAFQCAKLNDSSRVDKLRSWILNLEFDYFAIPKPDISIFLDVPFEFTSKRLSEQRDGA
                     DREYLNGRDDIHEKDLNFQQRVREVYLLQQSLDPQFVVIPCYDEHGSMLEPDKIFNRI
                     VEAILVHKALVI"
     gene            1304..>1732
                     /locus_tag="PLX53_07495"
     CDS             1304..>1732
                     /locus_tag="PLX53_07495"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOY72960.1"
                     /translation="MEQPPSYLKQRKTMTSNGRIISLDKPLVMGIINVTDDSFYSGSR
                     YKLGFRIAARAEQIIREGGSIIDVGACSTRPGAKPVSEQQELKRLAKALHAIRKKYPE
                     AIISVDTYRSRVARMAVGDYRANIINDISAGELDPEMFSTI"
BASE COUNT          578 a          385 c          372 g          397 t
ORIGIN      
        1 aagaaaagcc acaacacaga gaacagcaaa catagcagac tgttttttct cgccaaatga
       61 aggaggaagt gacgcccggt acctgaagaa agtatatgca gccgacagga ggacaatatt
      121 tttgaaaaat gtttgccaat tggtcaggtg tagggcatcg ccaaaacagc cgcaatccga
      181 aaccggatta aaaatagcca ggataagggt aaggggcgta aaggcaagca tgaatataaa
      241 aacaccccat gcagcaatcc gaagatttac accataaagc aacattaagc caatgagcag
      301 ctcagctgcg caaagaataa aggcgaaagc tagcgacagg ggctgcataa aattcatgtg
      361 aaaagccata aagtagtcat gaaacttata ggctgaaccc atgggatcta tgccttttac
      421 aaatcccgaa aaaataaata ccacggctaa aaggtacctg ctaatgataa cgacctgctt
      481 cataagtaga aacttttcaa tttaaatgtc aaattactaa tgctttatgt accagaatgg
      541 cctcaacaat tctgttaaaa attttatccg gctccagcat tgagccatgt tcgtcatagc
      601 aaggaataac aacaaattgt gggtcaagtg attgctgtag caggtaaacc tctcgaaccc
      661 gttgctggaa attcaaatcc ttttcgtgaa tatcgtccct cccgttaagg tattccctat
      721 cagcgccatc cctctgctcg gaaaggcgct tactggtaaa ttcaaatgga acgtctaaaa
      781 aaatactaat atcgggtttc ggaatggcaa aatagtcaaa ttcaaggttt aaaatccagc
      841 ttcttagctt atcaacccta ctgctgtcgt taagtttagc gcactgaaag gcaatgttgg
      901 agtaaacata gcggtcgagc agaaccacct cgtcagtttt aagccacgat ttaattaaac
      961 ccgacgcatc catccggtca agcgcataaa gcatggctac cacataggga tcaacctcat
     1021 caattctacc caaatcgcct cgaaggaaac gggcaatcag ctctccaaag tatggtgcgt
     1081 caaccctggg aaaatgaagg aaatggctgg caacaccctt ttgggttaaa aaagaaccca
     1141 gcaggtttat ttgagttgac ttaccagcgc catcgagacc ttcaataaca atcagtgcca
     1201 tgagtaaaat gtttaccttt gcagcaaaaa tcaacttgct gtggcaaaat tacatcaatg
     1261 tttagtaact ttagccatcg aacaagccgc aatatagcaa aaaatggaac aaccgccgtc
     1321 gtatctcaag cagaggaaaa ccatgaccag caatggccga attatcagct tggataaacc
     1381 actggttatg ggaataatca atgtaaccga cgattcattc tatagtggaa gtcgctacaa
     1441 actggggttt agaatagccg cacgcgccga gcaaattatt cgtgagggtg gaagcattat
     1501 agatgtagga gcatgctcca ctcgcccggg agctaaaccc gttagcgagc agcaggagct
     1561 taaacggcta gcgaaagccc tgcatgcaat ccgaaagaaa tatcccgaag ctataatttc
     1621 ggttgatacc tacagatcga gggttgcccg tatggctgtt ggcgattatc gggcgaacat
     1681 cataaacgac atttcagctg gagagcttga ccctgaaatg ttcagcacca tt
//
LOCUS       DAOOQX010000468         1728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_88690_length_1728_cov_3.488344, whole genome shotgun sequence.
ACCESSION   DAOOQX010000468 DAOOQX010000000
VERSION     DAOOQX010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88690_length_1728_cov_3.488344"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PLX53_07500"
     CDS             <1..657
                     /locus_tag="PLX53_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOY72961.1"
                     /translation="ALANNSAASSLRMLTEKNPVAEKNVRITYSVSTNHPIRNLTVYY
                     IPSRYPTRIEKQKGVVLECIDAERPNPTVKVENKSLRELTNEIIESHGCYEVLLVNAQ
                     GEITEGSRSNVFFISRDRLFTAPETKVLSGITRQKVVQICHSLNVELVERCIGIKELA
                     FMDGCFLTGTSPGVLPVRQVGNVSMNASIPVINAISMEYERLVEKSKAEWHSFTGSSK
                     "
     gene            831..1598
                     /locus_tag="PLX53_07505"
     CDS             831..1598
                     /locus_tag="PLX53_07505"
                     /inference="COORDINATES: protein motif:HMM:NF007757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOY72962.1"
                     /translation="MKISIIQASPFWESIEENLQLFTAKIESTSTDTNLILLPEMFTT
                     GFTMKPRECDASEAERTLDWMLQTSMVRKAAIVGSIIARKENKLVNRMAFVMPNGQVV
                     FYDKRHLFRMAGEHNQYSAGNQRVVVSYMGWRILLTICYDLRFPVWCRNRNDYDLMLN
                     VANFPDERSYAWNSLLVARAIENQCFVAACNRVGIDGSGTNYSGDSQVVDPMGQIVAK
                     ANPNSESTIEASISLEQLHEIRASFPAHLDADRFEIL"
BASE COUNT          535 a          375 c          383 g          435 t
ORIGIN      
        1 gcattggcaa ataattcagc tgccagttcc cttagaatgc tcactgagaa aaaccctgta
       61 gctgaaaaga atgtccgtat aacctacagc gtatccacca atcatccgat ccggaattta
      121 acggtttact acattccttc aaggtatccc accagaattg aaaaacagaa aggcgttgtt
      181 ctggaatgta ttgatgccga aaggcctaac ccgacggtta aagttgagaa caaatcgctg
      241 cgcgagctta ccaacgagat catagaaagt catggatgct acgaggttct tttggtaaac
      301 gcccaggggg aaattaccga aggaagccgt tccaatgttt tctttatcag ccgtgataga
      361 ttattcactg cgccagaaac taaagtgcta agtggaatta ccagacaaaa agttgtgcaa
      421 atatgccata gcttgaacgt tgaactagta gaaagatgta taggcattaa ggagctggcg
      481 tttatggatg gttgctttct tacaggaact tcaccaggag ttctacccgt tagacaggtt
      541 ggtaacgtga gcatgaacgc ctccatacct gtaattaacg ctatatcaat ggagtatgaa
      601 aggctggttg agaaatcaaa agccgaatgg catagtttca caggaagcag caaataagcc
      661 ctgcaaagaa aggtagaaat ctccggggag tgttcaatta agcattgagc agttgaccaa
      721 gcgctttttc acaacagcca agtgcctata ccaagcctaa aacttaaccc agaaaaaaat
      781 ttaggtatat tcgcagcatt ataccgaata gccaacacct aagctgcacg atgaaaatat
      841 ccatcattca agcatcgccg ttttgggaga gcatagagga aaaccttcag ctttttaccg
      901 ccaaaattga aagcacttca acagatacca acttgattct gcttcccgaa atgtttacca
      961 ccggtttcac catgaagccc agggaatgcg atgcaagtga agccgagcga actcttgatt
     1021 ggatgttgca aacctccatg gtaaggaaag ccgctattgt tggaagcatt attgcccgga
     1081 aagaaaacaa actcgtgaac agaatggctt ttgttatgcc caacggacag gttgttttct
     1141 atgataaacg ccacctcttc aggatggccg gtgagcacaa ccaatactcc gccggtaacc
     1201 aaagggtagt agtaagctac atgggctggc gaatacttct taccatttgc tacgatttgc
     1261 gtttccctgt ttggtgtcga aacaggaatg actacgacct gatgctcaac gtagctaact
     1321 ttccggatga acgaagctat gcgtggaatt ccctactggt tgcacgggct atcgaaaatc
     1381 agtgctttgt agccgcctgc aatcgagtgg gcattgatgg cagcgggacg aattattctg
     1441 gtgattctca ggtagttgac ccaatgggac aaattgtagc taaggcaaac cccaacagcg
     1501 aatcaactat tgaagcttca atctcattgg agcaactcca tgaaattcgt gcaagttttc
     1561 cggctcatct tgatgccgac aggtttgaaa ttctttaaag gagaaaacca tgcctgcaag
     1621 acatttattg taaagttttc gcttaacttt tcttccccga aattcatgta aacccttgta
     1681 tgttaagggt gtcaaaaatc acccgaaaga ttaaaaaaaa aaccgggt
//
LOCUS       DAOOQX010000469         1726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_88865_length_1726_cov_2.748055, whole genome shotgun sequence.
ACCESSION   DAOOQX010000469 DAOOQX010000000
VERSION     DAOOQX010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88865_length_1726_cov_2.748055"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /gene="gltX"
                     /locus_tag="PLX53_07510"
     CDS             complement(<1..836)
                     /gene="gltX"
                     /locus_tag="PLX53_07510"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOY72963.1"
                     /translation="MENRRVRVRFAPSPTGPLHIGGVRTALFNYFFARQHNGDFILRI
                     EDTDSQRFVPGAEEYINEALGWCGIKIDEGVKEGGLHAPYRQSERRQIYRQYADRLVA
                     SGNAYYAFDKPETLQKLRNEAENRGEAFTYNYANRNSMENSHTLGEDEVRNRIEGGDQ
                     WVIRYKMPADEEVVMHDIIRGEIRVNTSTLDDKVLFKSSDMLPTYHLANVTDDYLMEI
                     SHVIRGEEWLPSLPLHVLLYRALGWEDKMPQFAHLPLLLKPTGNGKLSKRDGDKMGFP
                     VFP"
     gene            complement(933..>1726)
                     /locus_tag="PLX53_07515"
     CDS             complement(933..>1726)
                     /locus_tag="PLX53_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOY72964.1"
                     /translation="VDYVVCDEVEAKVKVAVYSNLSCEIAVSNLQPTAWAVRQSSPEL
                     LKRINLWLENYLSTSKYARIRSKYYENPLQARRMSNDYFYVYQGKVSQWDEYFKHNSE
                     KIGWDWRLLASMVYQESHFDHNAVSPRGAVGLMQFMPTTADYFGIALNEMPIRQIEAG
                     VGYLKWIEDRLSSFNIPKKEEVKFVLAAYNAGIGHVIDARNLARKYGRNPNVWDDNVE
                     YFISNKAQFSGDPVVKYGSLKGKETTRYVEEILERYQHYQNIVEK"
BASE COUNT          449 a          424 c          361 g          492 t
ORIGIN      
        1 ggaaacacag gaaatcccat tttgtctcca tcacgcttgc ttagctttcc attaccggta
       61 ggtttaagca gtaggggcaa atgggcaaac tgtggcattt tgtcctccca tccaagggcg
      121 cggtataaca aaacgtgcag gggtagcgat ggaagccatt cctctcctct gataacatgc
      181 gaaatttcca tcaggtagtc atcggttaca ttggccagat ggtaggttgg aagcatgtcg
      241 ctactcttaa acagcacctt atcgtcaagc gtggaggtgt taaccctaat ctcgcctcga
      301 atgatgtcgt gcatgacaac ctcctcgtca gcaggcattt tatacctaat aacccactgg
      361 tcgccgcctt caattcgatt gcgaacctca tcctcgccta aagtatgcga gttttccatg
      421 gagttgcggt tggcataatt ataggtaaaa gcttctcccc tgttttctgc ctcattgcgg
      481 agtttctgaa gtgtttcggg tttatcaaac gcatagtaag catttccact agccaccagc
      541 ctatcggcat attgtctgta aatttgccta cgttcgcttt ggcggtaggg agcatgaagc
      601 ccaccctctt taactccttc gtcaatttta attccgcacc agccaagcgc ctcgttgata
      661 tactcctcgg cacctggtac aaaccgctga gaatcagtat cctcaattct aaggataaaa
      721 tcgccgttat gctggcgggc aaaaaagtag ttgaacaggg ctgttctaac ccctccaatg
      781 tgcaacggtc cggttggact aggggcaaaa cgaacccgta ctcttcggtt ctccatttca
      841 aattttttgt gaggtcaaaa ttatgaataa taagtgggct ataaacgtca acccgaaaaa
      901 acaagtacgg ctggctacaa aaaatctacc atttactttt ccacaatgtt ttggtagtgc
      961 tggtaccttt cgaggatttc ttcaacatac cgtgtagttt ccttaccctt aagacttcca
     1021 tatttgacta cgggatcgcc agaaaattgg gccttattgg atatgaagta ctcaacatta
     1081 tcgtcccaaa cattagggtt acggccatac tttctggcca agttacgggc atctataaca
     1141 tgacctattc cggcgttata agcagccagc acaaatttaa cctcttcttt tttagggata
     1201 ttgaagctgc tcagcctgtc ctctatccat ttaaggtacc caacaccagc ctctatctgc
     1261 cttataggca tttcattaag ggctatacca aaataatcgg cagttgtggg catgaattgc
     1321 ataagaccta ctgcgcctct gggtgatacg gcattatggt caaagtgcga ttcctgataa
     1381 accatggagg ctagtaaccg ccaatcccaa cctatctttt cactattatg tttaaagtat
     1441 tcatcccatt gcgaaacctt cccctggtaa acataaaaat agtcgttgct catgcgccgt
     1501 gcctgaaggg gattttcata atattttgaa cgtatccggg catacttcga ggttgacagg
     1561 tagttctcca gccacaaatt aatccgcttg aggagctcgg gtgatgattg acgaactgcc
     1621 catgctgtcg gttgaagatt actaactgca atttcgcagc taaggttact gtaaacggca
     1681 accttgactt tggcttccac ttcgtcgcag accacatagt caacct
//
LOCUS       DAOOQX010000470         1723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_89093_length_1723_cov_3.248201, whole genome shotgun sequence.
ACCESSION   DAOOQX010000470 DAOOQX010000000
VERSION     DAOOQX010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89093_length_1723_cov_3.248201"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLX53_07520"
     CDS             <1..436
                     /locus_tag="PLX53_07520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOY72965.1"
                     /translation="WPGLILRVVAATIVIVVFSYYLLPDKFLIMPREQPLMWAMIMIF
                     YPVWSAFTQEVIYRTYFFHRYGAAIPNEKVLALVNGLLFGFLHIIFRNWVAVIGATLM
                     GLLWAFSYLKHRSLLVVSIEHSIVGDMLYTFGLGYFFYVPDF"
     gene            complement(566..966)
                     /gene="ssrA"
                     /locus_tag="PLX53_07525"
     tmRNA           complement(566..966)
                     /gene="ssrA"
                     /locus_tag="PLX53_07525"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1049..>1723)
                     /gene="rho"
                     /locus_tag="PLX53_07530"
     CDS             complement(1049..>1723)
                     /gene="rho"
                     /locus_tag="PLX53_07530"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009041217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOY72966.1"
                     /translation="ANHPEVYMIVLLIDERPEEVTDMARSVNAEVISSTFDEPAERHV
                     RVANIVLEKAKRLVECGHDVVILLDSITRLARAFNTVQPASGKVLSGGVDANALQKPK
                     RFFGAARNIENGGSLTILATALTDTGSKMDDVIFEEFKGTGNMELQLDRKLSNKRIFP
                     SVDITASSTRREDLLLSKEVLQKIWILRNYLTDMNSVEAMEFLRDRLVKTATNEEFLW
                     SMNSDI"
BASE COUNT          414 a          414 c          417 g          478 t
ORIGIN      
        1 ctggcctggg ttgatactgc gtgttgtggc agcaaccatt gtaatcgttg tattcagcta
       61 ctacctgttg cccgacaaat tcctcataat gccgcgtgag cagcctctaa tgtgggctat
      121 gattatgatt ttttaccctg tttggtctgc ttttacccag gaggtgattt acagaacata
      181 cttttttcac cggtacgggg cagctatacc aaatgaaaaa gttctggcgt tggtgaatgg
      241 gttgctattc ggttttcttc atatcatttt ccgaaactgg gtggcagtaa tcggagctac
      301 gcttatgggg ctgctgtggg ctttcagcta cctgaagcat cgctcgttac ttgttgtttc
      361 tattgagcat tcaatagttg gcgatatgct ctacaccttc ggacttggat atttcttcta
      421 tgttcccgat ttctgaaagc ctctctccaa aataaaaaaa atggaaagta taacaaagac
      481 ttctggagta agggtatatt cttgaaataa aaaaagcgct ccaaattata acatttgagc
      541 gctttgggcc atcatacggg catgctggtg gagctggagg gaatcgaacc ctcgtccaaa
      601 caaggaacca gcttgttttc tacatgctta gcctttcttg gttgtcggga ctagccaggc
      661 gaaaggcaac cgaaccaatc cttaaccttt attattttac ccaagcttaa aggtgtcgct
      721 tgagctatcc cggttttgtc ggtgccccgt atgccggaaa caatcgggcg gaatttccgg
      781 cgggacatct cgtccccaca cacctaggcg tagggattaa gccattttgc tttgcaaaat
      841 taagcagcga gagcgtaatt tgattcgccg tttatttggt tgtgacctgt gattatcgag
      901 ccagcatcac gacgctcggc atgcttacaa acccattcta cttgctgtca aaaccacgtc
      961 agccccaatg gggttttagc aacggcaaag atacaataaa aaaccgccgg atatcaacat
     1021 tcggcggtta aagtttgcaa aaataaagct atatatcgct attcatcgac cagaggaact
     1081 cctcgttggt ggctgtttta accagcctgt ccctgagaaa ctccatggct tccaccgagt
     1141 tcatgtcggt gaggtagttg cgaagaatcc aaattttttg gagaacctcc ttacttaaca
     1201 gcaggtcttc gcggcgggtg ctggatgccg taatgtcaac cgaggggaat attcgcttgt
     1261 tggatagctt gcggtcgagc tggagctcca tgttgccggt tcccttaaac tcctcaaaaa
     1321 ttacatcatc catcttgctg ccggtatcgg taagagcagt ggccaaaatg gtaagcgaac
     1381 cgccgttttc aatgtttcga gctgcgccga agaaacgctt tggtttctgc agggcattgg
     1441 catcaacacc acccgagagc accttgcccg atgcgggctg aacggtgttg aacgcgcgtg
     1501 ccaggcgggt aatcgagtcg agcaggatta ctacatcgtg accgcactcc actagccttt
     1561 tagccttttc aagaactatg ttggccaccc ttacgtgccg ttcggcaggc tcgtcgaagg
     1621 ttgatgaaat tacctcggcg ttgacgctac gcgccatatc ggttacctcc tcgggtcgtt
     1681 cgtcaatcag cagcacaatc atgtaaacct ccggatggtt ggc
//
LOCUS       DAOOQX010000471         1719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_89509_length_1719_cov_2.003606, whole genome shotgun sequence.
ACCESSION   DAOOQX010000471 DAOOQX010000000
VERSION     DAOOQX010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89509_length_1719_cov_2.003606"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..609
                     /locus_tag="PLX53_07535"
     CDS             61..609
                     /locus_tag="PLX53_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOY72967.1"
                     /translation="MFEGFDYFHVEGYLVQNHEMLLRALEIASAKGLQISFDLASYNV
                     VEENRDFLNEIVKKYVTILFANEDEAKAFTGKKPREAVEVLAEIVPIAVVKLGPSGSY
                     IKRNSDFYQVGIIDVSSIDTTGAGDLYASGFLYGLMKGLPLDKCGQIGAILSGHVIEV
                     LGPKMDGERWTTIRRMVAEAET"
     gene            complement(704..1144)
                     /locus_tag="PLX53_07540"
     CDS             complement(704..1144)
                     /locus_tag="PLX53_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72968.1"
                     /translation="MKRILLAVAIIWVGLAFTARNANAQSEEELVQICGMIAGEAQYL
                     KDFRVKLDAGDPPPVQRIPILLKKDVKYRFTVCNSKDYEGKVVLQLFDNNRMLATTYV
                     IGTGKDYPSIDWVCTKTGAYHLFFSFRDGKAGLAVGLLSLVEMM"
     gene            complement(1159..>1719)
                     /locus_tag="PLX53_07545"
     CDS             complement(1159..>1719)
                     /locus_tag="PLX53_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72969.1"
                     /translation="VVDYISVIKRFADDIQVGQFFDFETLKRLATNKENLDSLILISQ
                     QSFNRIDSYLRETNRSTLSAVIVAGVWVEGAYLTSRVAQTSNHPRIRETIGEQKTVLG
                     FLLALLKAYSNDPNMAKLIAQLEPLKNQFDNVKITYELGEPEMIEVNGELTFIQKDKQ
                     LVDMDDVTYKEIISGIQTLRNSLIKL"
BASE COUNT          453 a          365 c          404 g          497 t
ORIGIN      
        1 tacttttgcg gtgtaccttg gagctgccct ggagctgacc gcagttgatt taaacccgaa
       61 atgttcgaag gttttgacta ctttcatgtt gaaggctacc tggttcaaaa ccacgaaatg
      121 ctactacgtg ctttggagat tgcttctgcc aaaggtcttc agatttcgtt cgatttggcc
      181 agctataacg ttgttgagga gaaccgtgat tttctcaacg agatagtaaa aaagtacgta
      241 accattcttt ttgccaatga ggatgaagcc aaggccttta caggcaagaa gcccagggag
      301 gcagttgaag tcttggcgga aattgtgccc atagccgtgg ttaagctggg cccttcaggg
      361 tcgtacataa agcgaaattc cgacttctac caagtgggca ttattgatgt ttcgagtata
      421 gatacaaccg gtgcgggcga tttatatgca tccggttttc tttacgggct gatgaaagga
      481 ttaccgcttg ataaatgcgg gcaaattggc gctattctgt cggggcatgt catagaggtg
      541 cttggcccca aaatggacgg ggaacgttgg actaccataa gaagaatggt tgcagaagcg
      601 gagacgtaat tagctaaaca aaaatggata tgctcaaacg cgctaaaaaa ggaactttaa
      661 taaatagttc ttttttgcta tgggtgtacg gtcttttata gatttacatc atctcgacaa
      721 gtgagagcag gcctactgcc agtccggcct taccgtccct gaagctaaag aaaaggtggt
      781 aggctccggt ttttgtgcac acccaatcaa tggatggata atcctttccg gtgcctatta
      841 cgtaggtggt tgcaagcata cgattgttgt caaaaagctg tagcacaact tttccctcat
      901 agtcctttga gttgcaaact gtgaaacggt atttcacatc tttctttaaa agaatgggaa
      961 ttcgctgaac aggtggcgga tcgcctgcat ccagcttaac cctgaagtct ttaaggtatt
     1021 gtgcttctcc tgcaatcatt ccgcatattt gaaccaactc ctcttcggat tgagcattag
     1081 cgtttcttgc tgtaaatgct agacccaccc aaataattgc aaccgctagt aatatccttt
     1141 tcatggccaa aaaatttcct acaatttaat gagcgaattt ctaagggttt gaatacctga
     1201 aataatctcc ttataagtta catcatccat atcaaccagc tgcttgtcct tctgaataaa
     1261 ggtaagctcc ccgttaactt caatcatctc gggttctccc agctcatagg taattttaac
     1321 gttatcaaac tggttcttaa gtggttcgag ttgagcaatg agttttgcca tgttggggtc
     1381 gttgctgtat gccttaagca gtgccagaag aaaccccaaa acggtttttt gctcgccaat
     1441 ggtttcgcgt atgcggggat ggttactggt ttgcgcaaca cgggaagtaa ggtaggcgcc
     1501 ttcgacccat acccccgcaa caataaccgc acttagcgtt gaacggttgg tttcgcgaag
     1561 gtagctatca atccggttaa aactttgctg ggagatcagg atgagggagt ccaggttttc
     1621 tttattggtt gccagtcttt taagtgtttc aaaatcgaag aattgaccca cctgtatgtc
     1681 gtcggcaaag cgtttgatca ccgagatgta gtcaaccac
//
LOCUS       DAOOQX010000472         1718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_89535_length_1718_cov_3.395069, whole genome shotgun sequence.
ACCESSION   DAOOQX010000472 DAOOQX010000000
VERSION     DAOOQX010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89535_length_1718_cov_3.395069"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PLX53_07550"
     CDS             complement(<1..1104)
                     /locus_tag="PLX53_07550"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY72970.1"
                     /translation="MKKVVTILLILGLSVAPGFLPGQTIPAALQAEMEKNIELARSYE
                     TAGDINSALPLYTKTATTYWVNGNSEEARKLFIKAIALSERIGNTNALKMLLTNLGML
                     YVDEENYPEAIVNFEKCLSLNRKQNNKTEIASTLLNIANAQKELGQLQKSLNAAEEAS
                     KIAAELNDPKLMRNSYSLLSELYKEAGNDEKSAEYFTLYSAFSKKIQREEQLKKENEA
                     KKIVSEAQSQLHEIKTEKELTEQELMAKQHALTTVKDSLQKVEQLTREQRMEIDLLAK
                     ENALRDARIKNQILIRNIFIIAIILTLGIAGLITYYYLQKKRSNELLAHQKDMLEVKS
                     NELMEAIKQVEKQNRDITSSINYAQRIQQALLPT"
     gene            complement(1277..1654)
                     /locus_tag="PLX53_07555"
     CDS             complement(1277..1654)
                     /locus_tag="PLX53_07555"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOY72971.1"
                     /translation="MDKLIIESRIDTPKVVADGNTGLFEITGKSLPEDAFEFYKPLER
                     YVQGYIKQPQQKTTINLKLEYLNSSSSKKLLDVIGYFEQLSKHGYEIELNWYHHDEDQ
                     DMIDEGTEFANMTSLRVNFIAEH"
BASE COUNT          375 a          360 c          402 g          581 t
ORIGIN      
        1 ggttggaagc agggcttgct gaattcgctg ggcatagtta atgctgctcg tgatgtcgcg
       61 gttttgcttt tcaacctgct taatggcttc cattagctca ttacttttta cctccagcat
      121 gtccttttgg tgtgccagga gctcgttgga gcgtttcttt tgcaggtagt agtaggtaat
      181 aagccctgca atgcccaggg tgagtattat ggcaatgatg aatatgttac ggataagaat
      241 ttggtttttt attctggcat cccttaaggc attttccttg gcaagtaggt caatctccat
      301 acgctgctcg cgggtgagtt gctctacctt ttgcaggctg tccttaacgg ttgttagcgc
      361 atgctgtttt gccatcaact cttgctcggt aagctccttt tctgttttta tttcgtgaag
      421 ctggctttgg gcttcgctaa ctattttttt agcctcattt tcctttttaa gctgctcctc
      481 gcgttgaatt ttttttgaaa atgcggagta gagcgtaaag tattcagccg atttttcgtc
      541 gttgccggct tccttataga gttccgaaag caacgagtat gagtttcgca tcagcttggg
      601 gtcgttcagc tcggctgcaa ttttgctggc ttcctctgcc gcgttgagtg atttttgaag
      661 ctgccccaac tccttttggg cattggctat gtttagcagg gttgaggcta tttccgtttt
      721 gttgttttgc tttcggttga gcgataggca tttctcaaag ttgacaatgg cctcggggta
      781 gttttcctcg tcaacataca acatgccaag gttggtcaaa agcattttga gggcattggt
      841 atttccaata cgctcgctaa gcgcaattgc tttgataaac aattttcttg cctcctcgga
      901 attaccgttt acccagtagg tggtggctgt tttggtgtaa aggggtaggg cggagttaat
      961 gtcgcctgca gtttcgtagc ttctggccaa ctcaatattt ttctccattt cggcctgaag
     1021 ggctgcagga atggtttgtc cagggagaaa accaggtgca acgctaagtc caagaattaa
     1081 aagtatggtg acaacttttt tcacaacata ttcggtatta tgtgtgctcc taaataattt
     1141 gcttttgaaa actcgaactc ctacttgcct tcttgtgcgt tagccctgtt agcgccttat
     1201 acaccggtta caggacaaaa tctggattag tgtgatatta ctactaagtg cttgctgcac
     1261 aaccgcatgg gtatgcctag tgctcggcaa taaaatttac ccgaagggag gtcatgttgg
     1321 caaactctgt accctcgtct atcatatcct gatcctcgtc gtgatggtac cagttaagct
     1381 caatttcgta accatgcttt gatagctgct caaagtaacc aatcacatca agcagctttt
     1441 tggaggatga ggagttgagg tactccagct taaggtttat tgtggttttt tgttggggct
     1501 gtttgatgta tccctgaaca tacctttcca gaggcttgta gaactcaaag gcatcttcgg
     1561 gtagcgactt gccggtaatt tcaaacaagc cggtattgcc atcagccaca acttttggag
     1621 tatctatccg gctttcaatt attagtttat ccatgttgct cattttattt caatatttac
     1681 gcaaaattgt aaccaaaggt tttaccataa gcagggca
//
LOCUS       DAOOQX010000473         1717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_89624_length_1717_cov_3.129362, whole genome shotgun sequence.
ACCESSION   DAOOQX010000473 DAOOQX010000000
VERSION     DAOOQX010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89624_length_1717_cov_3.129362"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PLX53_07560"
     CDS             complement(<1..865)
                     /locus_tag="PLX53_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOY72972.1"
                     /translation="MGKFKKVNNITGWIVFAISAVVYLLTIEPTASFWDCGEFIATAF
                     KQEVGHPPGAPLFMMVARFFTLFASDVTKVAALVNAMSALASAFTILFLFWTITHLAR
                     KLIIKDAENISTGNLVAVMASGVVGALAYTFSDTFWFSAVEGEVYAMSSFFTAIVVWA
                     MLKWEEVADEPHSNRWLIFIAYMMGLSIGVHLLNLLTIPALAFIYYYKKYKPTRNGIF
                     VALLVSAVILVAILYIIIPGTFKLGSLFELMFVNGFGLPYFSGLIFYFILLFTLIAWG
                     IYNSHKKKKVLL"
     gene            1130..1477
                     /locus_tag="PLX53_07565"
     CDS             1130..1477
                     /locus_tag="PLX53_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOY72973.1"
                     /translation="MKREEQDNDKLKFAYSTNPDFKASNEMQPEQESISPEKQKLVVC
                     IERKNRGGKCVTLVKGFRGSNDDLDTLGKQLKARCGVGGSVKDGEIIIQGELRQKVAA
                     ILRSYGYNVVVSG"
     gene            1531..>1717
                     /locus_tag="PLX53_07570"
     CDS             1531..>1717
                     /locus_tag="PLX53_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72974.1"
                     /translation="MILSFGAAADTLTVMHYNLMYYDREYEECTAANNNVDSKDEYLR
                     TIIGYARPDILTVNEVNA"
BASE COUNT          569 a          328 c          388 g          432 t
ORIGIN      
        1 ttagcagcac tttcttcttt ttatgggagt tgtaaattcc ccatgcaata agggtaaata
       61 gtagtatgaa gtagaaaatc agcccgctga agtagggtaa tccaaaccca ttaacaaaca
      121 ttagctcaaa gagggagcca agtttaaatg ttccggggat tataatgtaa agtatcgcca
      181 ccaggataac agctgaaact agcagggcca caaaaatacc gttgcgcgta ggcttgtatt
      241 ttttgtagta gtaaataaat gcgagcgccg gaatggtgag caggttgagc aggtgaacgc
      301 caatggataa tcccatcatg taggcaataa aaatcagcca gcggttggaa tggggctcat
      361 cagcaacctc ttcccacttt aacattgccc aaacaacaat ggcggtaaag aacgatgaca
      421 tggcgtaaac ctcgccctct acggccgaga accagaaagt gtcggaaaaa gtataggcaa
      481 gtgcacctac tactccgctt gccataacag caacaagatt tccggtagaa atattttcgg
      541 catctttgat gattagcttg cgggcaagat gggtgatggt ccaaaaaaga aacaggattg
      601 taaaggcact agctaatgcc gacatggcat ttaccaatgc tgccaccttc gtaacatcgg
      661 atgcaaacag ggtaaagaaa cgggcaacca tcataaaaag aggcgcacca ggaggatgac
      721 ccacttcttg tttaaaggct gtggcgatga attccccgca gtcccaaaaa cttgcggtag
      781 gttcaatagt caaaaggtac actactgctg aaatagcaaa tactatccaa ccagtaatgt
      841 tgtttacttt tttgaatttt cccataggaa agttggtttt gcaaacaatt taacacaatt
      901 atggcaacaa atgtagaatt tttagaagaa taatggtttg aaaaatactg aaatctttgg
      961 gctaaccatc tgctaccaac gaaaaccaca aaaatttact ttaccattag cagctcttta
     1021 aggttgatta ctaacattta ttaaactttg cgcataactt tgcaacatgc cagaagcaga
     1081 gtgtctggtt aaggtagcat taatcaggag ctaactattt ataggcaaaa tgaagagaga
     1141 ggaacaggat aatgacaagt tgaagttcgc ctattcaacc aaccccgatt ttaaggcgag
     1201 taatgaaatg caaccggagc aagaaagtat ttcgcccgaa aagcaaaaat tggtggtatg
     1261 cattgaacgg aaaaacagag gtggaaaatg cgtaacttta gttaagggat tccggggtag
     1321 caacgatgac ctagacacat tagggaaaca actcaaagct agatgtgggg ttggtggatc
     1381 ggttaaggat ggagaaatta tcattcaggg tgagctgcgg caaaaggttg ccgcaattct
     1441 ccgttcttat gggtataatg ttgtagtttc aggttagaaa acaaccctaa ggcattgact
     1501 atgaaattta tatttcaact tttgatattt ttgattctat cctttggtgc tgctgccgat
     1561 acgcttacag tcatgcatta caacctgatg tactacgacc gggaatatga ggagtgtacg
     1621 gcagcaaaca acaatgttga cagcaaggac gaatatctgc gaaccattat tggctatgct
     1681 aggcccgaca tactaaccgt taacgaggta aatgcat
//
LOCUS       DAOOQX010000474         1716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_89695_length_1716_cov_3.827213, whole genome shotgun sequence.
ACCESSION   DAOOQX010000474 DAOOQX010000000
VERSION     DAOOQX010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89695_length_1716_cov_3.827213"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1716
                     /gene="htpG"
                     /locus_tag="PLX53_07575"
     CDS             <1..>1716
                     /gene="htpG"
                     /locus_tag="PLX53_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOY72975.1"
                     /translation="DKYINQIAFSGAEEFMEKYKNQANGIIGHFGLGFYSSFMVSDKV
                     EIVTKSWRDDAGAVKWSCDGSPEFAIEEATRSSRGTDVILHISDESKEFLEESRISEL
                     LLKYCRFLPIPIAFGTIKEWKDGKEVDTGKEKIINETNPLWVRKPVDLTEEDYMNFYR
                     NLYPTQEDPLFYIHLNVDYPFHLTGILYFPRIKTNFDIQKNRIQLYSNQVYVTDSVEG
                     IVPEFLTLLHGVIDSPDIPLNVSRSYLQSDSNVKKISSHITKKVADRLQEIFKNDREQ
                     FEKKWDDLKLFITFGMITDEKFYERAEKFALLKNVDGKYFTLEEYEKLVKDNQTDRNK
                     NVIYLYASDREAQHSYIEEAKNRGYDVLLIDGQLDPHFINTLESKLKDTRFARVDSDV
                     VDKLISKEETREVSLSAEEQDDLRAVFEGALPEGDHYWVSFEELGETANPVIITHSEF
                     MRRMKDMAAMSPTASFYDKMPDSHNLVVNTAHPLVKAIVEAKNKQVGGKLKELNAGIE
                     PLKVSVDELHKAQMEKKEEEIPQAQKEELAEREKQLEELKEERRNLLKNFGREHKLVK
                     ELTDLA"
BASE COUNT          480 a          404 c          453 g          379 t
ORIGIN      
        1 tgataagtac atcaaccaga ttgccttctc gggcgccgag gagtttatgg agaagtacaa
       61 gaatcaggcc aacggcatca tcggacactt tggccttggg ttttactcat cattcatggt
      121 gagcgacaag gtggaaattg taaccaaatc gtggcgcgac gatgccggag cagttaaatg
      181 gtcatgtgac ggctcacccg agtttgccat tgaggaggca acccgcagct cgcgtggaac
      241 cgatgttatt cttcacatca gcgatgagtc gaaggaattt cttgaggagt cgcgcattag
      301 cgagctgctg ctcaagtact gtcgttttct acccattccc attgcctttg gcaccatcaa
      361 ggagtggaag gatggcaagg aggttgacac cggcaaggaa aaaatcatca acgaaaccaa
      421 cccactttgg gtgcgtaagc cggttgacct caccgaggag gactacatga acttttaccg
      481 caatctctat ccaacgcagg aggaccctct attttatatc cacctgaacg tggattaccc
      541 tttccacctc accggaattc tctacttccc acgcataaaa accaacttcg acattcaaaa
      601 aaacaggata cagctctact ccaaccaggt ttacgttacc gattcggttg aaggtattgt
      661 gcccgaattt ctaaccctgc tgcacggggt gatagactcc cccgatattc cccttaacgt
      721 ttcgcgaagc tacctgcaaa gcgattccaa cgtaaagaaa atatcgagcc atatcaccaa
      781 aaaggtggcc gacaggctgc aggaaatttt taagaacgac cgcgagcagt tcgagaagaa
      841 gtgggacgac ctgaagctgt tcatcacctt cggaatgatt accgacgaga agttctacga
      901 acgtgccgag aagtttgccc ttctcaagaa tgtggatggg aagtacttca cccttgagga
      961 gtacgagaag ctggttaagg acaaccagac cgaccggaat aagaatgtaa tttaccttta
     1021 tgccagcgat agggaagccc agcacagcta cattgaggag gctaagaacc gtgggtacga
     1081 cgttctgctg atagatgggc agctcgatcc gcacttcata aacacgctgg agagcaagct
     1141 taaggatacc cgttttgcca gggttgactc cgacgtggtt gacaagctga tatccaagga
     1201 ggaaacccgt gaggttagcc tgtctgctga agagcaggat gacttgcggg ctgttttcga
     1261 gggtgcgctt cccgaaggcg accattactg ggtttcgttt gaagagctgg gcgaaaccgc
     1321 taaccccgtt atcattactc attcggagtt catgcgccga atgaaggata tggctgccat
     1381 gagcccaacc gctagctttt acgataaaat gcccgatagc cacaacctgg tggtgaacac
     1441 cgcacacccg ttggtgaaag ccattgttga agctaagaat aagcaggttg gcggtaagct
     1501 gaaggagcta aacgccggca ttgagcctct aaaggttagc gttgatgagc tgcataaagc
     1561 ccagatggaa aagaaggagg aagaaattcc tcaggctcag aaggaggaac ttgccgaaag
     1621 ggagaagcag ctcgaggagc tgaaggagga gaggcgcaat ttactcaaga attttggacg
     1681 tgagcataag ctggttaagg agctaactga tttggc
//
LOCUS       DAOOQX010000475         1713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_90013_length_1713_cov_2.471653, whole genome shotgun sequence.
ACCESSION   DAOOQX010000475 DAOOQX010000000
VERSION     DAOOQX010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90013_length_1713_cov_2.471653"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="PLX53_07580"
     CDS             complement(<1..1297)
                     /locus_tag="PLX53_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOY72976.1"
                     /translation="MEKKLIILAVTIPMLFYACQKNRIQYPETKRVDVVDNYFGTKVP
                     DPYRWLEDENSPEVAEWVSKQNELTSSYLAKIPFREKIRERLTGIWNYTTMGVPFKEG
                     GRYFFFKKDGRQNQSVLYVMETLNSEPRVLLDPNTFSADGTIALSEVSPSRDGKYLAY
                     AVNDGGSDWQKIKVIALPEGKELKDEISWVKFSSIAWYANGFYYSRYDEPKKGEALST
                     LNQYHKVYYHTMGTDSNEDKLIFANSQHPLRNYSAQTTENEKYLIIYETESTYGNSIY
                     IKNLTDKRGEFSQLTTGFEYEYSVLENVDDNLYVLTNYKAPKYKLIKININSMDVGNW
                     KDILPERKDVLTSCQLVGGKIIANYVVDAKSKAEVYTLSGEKTDEIHLPTIGTISGFS
                     GSYSDTLAFYSFSSFTVPPAVYKFNVAKNESTEFFKPDIK"
BASE COUNT          464 a          341 c          359 g          549 t
ORIGIN      
        1 atttgatatc gggcttgaaa aattcggttg attcattttt ggcaacgttg aacttgtaaa
       61 cagccggagg aacagtaaac gaggagaagc tgtagaaggc caacgtgtcg ctataggagc
      121 cgctgaaacc ggaaattgtt ccgatggttg gtaggtgaat ttcatcggtt ttttcaccac
      181 tgagggtgta aacctcggcc ttgctcttgg catctaccac gtagttagcg ataattttgc
      241 cacccactag ctggcaggag gtaagcacat ccttacgttc aggcaggatg tctttccagt
      301 ttccaacatc catgctgtta atgttgattt taataagctt gtattttgga gctttgtagt
      361 tggttagaac gtaaaggttg tcgtcaacat tttccaacac gctgtactca tactcgaaac
      421 cagttgtgag ttgagagaat tccccgcgct tgtcggtgag gtttttaatg tagatggagt
      481 taccgtaggt cgattcagtt tcgtagataa tcaggtattt ttcgttctcg gtagtttggg
      541 cgctatagtt ccttagggga tgctgactgt ttgcaaaaat cagcttgtct tcatttgaat
      601 cggtccccat ggtgtggtag taaactttat ggtactggtt aagggttgag agcgcttcac
      661 cttttttagg ctcgtcgtaa cggctgtagt aaaaaccgtt ggcataccac gcaattgacg
      721 agaacttaac ccaggaaatt tcgtctttca gctcttttcc ttcaggtaac gcaatcactt
      781 taatcttttg ccagtcagaa cctccgtcgt tcactgcata ggccaggtat ttaccatcgc
      841 gcgatgggct tacttccgaa agggctatgg taccatccgc actaaaggta ttggggtcaa
      901 gtagaactct tggttcagag tttagagttt ccataacgta gagcacactc tggttctgcc
      961 gtccatcctt cttgaaaaag aagtatcgcc ccccttcctt aaagggaacc cccatggtgg
     1021 tataattcca gatgccggtt agcctttccc gaatcttctc acggaatgga attttagcaa
     1081 gatacgatga ggttagctcg ttctgcttcg aaacccactc ggctacctca ggcgaatttt
     1141 catcttccaa ccagcggtag ggatcgggaa ccttagttcc aaagtagttg tctactacat
     1201 caaccctttt ggtttcggga tactgaatcc gatttttttg acaagcgtaa aacagcatgg
     1261 gtattgttac tgcaagtatg attaattttt tttccacgga tacaataatt tagttgaaca
     1321 tatttgaatg taatcgtatt ggcatcaaac tacctgaata cattgtagtg ttaataggat
     1381 attaaaatga ctacctttat actagcccaa aattatatta aattcatcag cttctcatag
     1441 atttgtcttc aagttaactt tcaagaactc tacccggggc ataaaacttc atcccaatca
     1501 ggctttccat aagaaattga agcggttaaa ggatatataa agttagccat gtttaagtac
     1561 gcctatgtta ttcaacaaaa gtataatatt tttaacaaag tattattatt gagcaccatt
     1621 gattaaagta ttttttaggc ataaggatag ttttgttatc cgacaattat taagtatcac
     1681 gttcccattt atttaatcta ataacatata agt
//
LOCUS       DAOOQX010000476         1711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_90171_length_1711_cov_3.116546, whole genome shotgun sequence.
ACCESSION   DAOOQX010000476 DAOOQX010000000
VERSION     DAOOQX010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90171_length_1711_cov_3.116546"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /gene="rfbB"
                     /locus_tag="PLX53_07585"
     CDS             <1..823
                     /gene="rfbB"
                     /locus_tag="PLX53_07585"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOY72977.1"
                     /translation="GVIHLAAESHVDRSIADPMSFIKTNIVGTTTLLNAALKSWGHNL
                     QNKLFYHISTDEVYGSLGSEGFFTEQTSYDPRSPYSASKASSDHLVRAYHHTYGLPTV
                     ISNCSNNYGPNQFPEKLIPLTINNIKNKKPIPVYGKGENIRDWLYVEDHAAAIDLVFH
                     RGEHGKTYNIGGNNEWKNIDLIRLLCRILDDKLDRQKGESEKLITFVRDRAGHDLRYA
                     IDSSKIRSELGWRPSVSFEEGLERTVDWYLENNAWLEEVVTGQYLTYYEKQYANR"
     gene            complement(935..>1711)
                     /locus_tag="PLX53_07590"
     CDS             complement(935..>1711)
                     /locus_tag="PLX53_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOY72978.1"
                     /translation="ETADTLAAIKLAKEAGALVLGICNVVGSSIPRETHAGVYTHAGP
                     EIGVASTKAFTAQVTVLIMMAILLGRKRGTLTEEHYQTLIQEMEKVPEKIEQILNSDS
                     IVKSISERFKTTNNFLYLGRGYYFPVALEGALKLKEISYIHAEGYPAAEMKHGPIALI
                     DDTMPVVVIATKDNSYEKIVSNIQEVKARKGVIIAIVTEGDTVIREMADYVIEIPEAV
                     EAIGALLAVIPLQLLAYHIALLRGCNVDQPRNLAKSVTVE"
BASE COUNT          457 a          418 c          395 g          441 t
ORIGIN      
        1 cggagttatc cacctggcag ccgagtcgca tgttgaccgc tccatagcag accccatgtc
       61 gttcattaaa accaacattg ttggaactac aacccttttg aatgctgccc tgaagagctg
      121 gggacacaac ctacaaaata agctgttcta ccacatttca accgatgagg tttacggcag
      181 cttaggcagc gaaggattct ttaccgagca aacatcatac gatccacgta gcccttactc
      241 cgcctcgaag gcctcttccg accacctggt acgagcctac caccatacct acgggttgcc
      301 aaccgttatt tcaaactgct ccaataacta cggtcccaac cagttcccgg agaagttgat
      361 tccgctcaca ataaacaaca ttaaaaacaa aaaacccatt ccggtttacg ggaaaggtga
      421 aaatattcgc gactggcttt acgttgaaga ccatgccgct gccattgacc tcgtttttca
      481 taggggcgaa cacggtaaaa cgtataatat tggaggaaac aacgagtgga agaatattga
      541 tctcatccga ttgctttgtc ggattttgga tgataagctg gacaggcaaa aaggagagtc
      601 tgaaaagctg attacctttg tcagggatag ggctggacac gacttgaggt atgcaataga
      661 ctccagcaag atccgtagcg agcttggatg gcgaccatcc gttagctttg aggaggggct
      721 tgagcgaacc gtggattggt atctggaaaa caacgcctgg ctagaggaag ttgttacagg
      781 ccagtactta acctattacg agaaacagta tgccaacagg tagcccttta gtgcttgact
      841 aaacatacaa acataaaaaa ggagatagaa agccatctcc tttttaattt taaaatgtgc
      901 ctgaaacaat ttctggagta tttttctgaa aattctactc caccgtaacc gatttggcca
      961 ggttgcgtgg ctggtcaaca ttgcaacccc taagcagggc aatgtggtag gctagcagct
     1021 gcaatggaat aacagccaac agggcgccaa ttgcctcaac ggcttcggga atttcgatca
     1081 cgtaatcggc catttccctg attaccgtgt ctccttcggt aactattgcg atgattacgc
     1141 cctttcgtgc tttaacctcc tggatgttgc tcacaatctt ttcatagctg ttgtccttag
     1201 tggcaataac cacaacgggc atcgtatcgt caattagcgc tattggccca tgcttcatct
     1261 ctgcagctgg atacccctca gcatgaatgt aggatatctc cttcagctta agcgctccct
     1321 caagggccac ggggaagtag tagcctctgc caaggtacag gaagttattg gttgtcttaa
     1381 atctctcgga aatgctttta acaattgaat cggagttaag tatctgctca attttttcgg
     1441 ggaccttctc catttcctga attaatgtct ggtagtgctc ctcggtaagt gttccccttt
     1501 tgcgtcccag caaaatggcc atcataatta gaacggtaac ctgagcggtg aaagctttag
     1561 tagaggcaac ccctatttcg gggccggcat gggtgtaaac gccggcatgg gtttcgcggg
     1621 gtatgctgga acccaccacg ttgcaaatac ccagcaccaa ggctcctgct tccttagcaa
     1681 gtttaatggc agctagggta tcggcagttt c
//
LOCUS       DAOOQX010000477         1708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_90441_length_1708_cov_2.652148, whole genome shotgun sequence.
ACCESSION   DAOOQX010000477 DAOOQX010000000
VERSION     DAOOQX010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90441_length_1708_cov_2.652148"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="PLX53_07595"
     CDS             complement(<1..1188)
                     /locus_tag="PLX53_07595"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOY72979.1"
                     /translation="MNQLKVDLSNIYTQVAKEEVFALKTKIAQNQLLLEQGTGKGSDF
                     LGWLNLPSSITKEQLYDIQHTASELRAKSDVVVIVGIGGSYLGAKAAIDSLSSSFTQL
                     TEDVLPKIIFAGNSICEDYHAELLELLETKRYSIVVISKSGTTTEPAITFRVLKQHLE
                     RQVGKTEAASRIVAITDLSKGALRKLADSEGYRTFVIPDDVGGRFSVLTPVGLLPIAI
                     AGIDIVKLVEGARKAMDDTSSSVAFDQNPACIYAAARNALYQKGKKVEILVNFTPRLH
                     YLTEWWKQLFGESEGKEGKGIFPAGVDFTTDLHSMGQYIQEGERMLFETVLSVEKPSH
                     RLVVPDDPDNLDQLNFLSGKRFSEVNLMAQMGTIAAHSDGGVPCISISIPELSPYWVG
                     YLFY"
     gene            complement(1218..>1708)
                     /locus_tag="PLX53_07600"
     CDS             complement(1218..>1708)
                     /locus_tag="PLX53_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HOY72980.1"
                     /translation="YFETHYIKTNIGTDIYGTEYSAVLKNIYAVASGIAHGLNYGDNF
                     LAVLISNAQREIERFLKKTYPSKRRLSTSVYLGDLLVTCYSQFSRNRTFGTMIGKGYS
                     VKSAMLEMSMVAEGYNAAKCIWEINKKYNVKMPIANAVYNILYENISPAIEMKLLTAK
                     LR"
BASE COUNT          444 a          409 c          390 g          465 t
ORIGIN      
        1 gtagaacaag taacccaccc agtagggtga aagttcggga atactgatag aaatgcaggg
       61 tacaccgcca tcgctatggg cagcaatggt tcccatttgg gccatcaggt tgacctccga
      121 gaagcgttta cccgagagga agtttagctg atcgaggtta tcgggatcgt ctgggacaac
      181 aaggcggtgg gagggttttt cgaccgaaag aacagtttcg aaaagcatac gttctccttc
      241 ctggatgtac tgccccatgg agtgaaggtc ggtggtgaag tcaacgccgg cggggaaaat
      301 tcccttacct tcctttccct cgctttcgcc aaaaagctgc ttccaccact cggtaaggta
      361 gtgaaggcgt ggggtgaagt taaccaaaat ttccaccttc ttgccctttt ggtaaagggc
      421 attgcgggct gcagcatata tgcatgcggg gttctggtca aaagctacag atgaagaggt
      481 gtcgtccatg gcttttctgg ccccttccac cagcttcaca atatcaattc cggcaatagc
      541 aatggggagt aaaccaacgg gtgttaaaac cgaaaacctt ccacccacat catcgggaat
      601 gacaaaagta cggtatccct cgctatcggc cagcttacgc agcgctcctt tcgacaggtc
      661 agttatagcc acaatacgac ttgcagcctc ggttttccca acctgtctct caagatgctg
      721 cttcagcacc ctaaaggtta tggcaggttc agtggttgtg cccgatttgg agataacaac
      781 aattgagtag cgcttagttt cgaggagttc gagcagttcg gcatggtaat cctcacaaat
      841 gctgtttcct gcaaaaatta ttttaggcaa aacgtcctcg gtaagctggg taaagctgct
      901 acttagtgaa tcaatagcgg cctttgcccc caggtatgaa cccccaatcc caactatcac
      961 cactacatca cttttagccc gaagctcact ggctgtatgt tgtatatcgt aaagttgttc
     1021 cttagtaata cttgagggta agttgagcca acccagaaaa tcactacctt taccggtacc
     1081 ctgctcaagg agcagctgat tttgcgctat tttggtttta agggcaaaga cttcctcctt
     1141 tgctacctgg gtataaatgt ttgataagtc aactttcaac tgattcattt ttataaatta
     1201 acgggttaaa atttcatcta ccttagcttt gctgtcaaca gcttcatctc aatagcaggc
     1261 gagatattct cgtaaagaat gttgtaaacg gcgttggcta tgggcatttt aacattatac
     1321 tttttattaa tctcccaaat gcacttagct gcattatacc cctcggccac catgctcatc
     1381 tccagcatgg ccgatttaac gctgtagccc ttcccaatca tggtgccgaa agtgcggttc
     1441 cggctaaact gcgagtagca ggtaaccagc aaatcgccca ggtagaccga agtacttagc
     1501 cttcgcttac tgggatatgt tttcttaaga aatcgctcaa tttcacgttg ggcattgctg
     1561 ataagcacgg ccaaaaagtt gtcgccatag ttaagcccgt gggcaattcc tgaggcaact
     1621 gcataaatat tcttaagcac agcgctatac tctgtgccat aaatatccgt tccaatatta
     1681 gttttgatgt agtgtgtttc aaagtagg
//
LOCUS       DAOOQX010000478         1704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_90764_length_1704_cov_3.150394, whole genome shotgun sequence.
ACCESSION   DAOOQX010000478 DAOOQX010000000
VERSION     DAOOQX010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90764_length_1704_cov_3.150394"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLX53_07605"
     CDS             <1..489
                     /locus_tag="PLX53_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HOY72981.1"
                     /translation="PLSELHFDPVKISISMFVSEVIYRTVREVEPNSELYEFLENSIR
                     LLDVMHDGVANFHLIFLIQLTRYLGFYPANGWSETMPFFDYKNGFFCPSPPSHSFNLD
                     KTSSRRLGMLLGTPFYRSNEISLNHAQRIQAINDLLCYYNFHLGSPLDFKTLPILIQL
                     FQ"
     gene            589..>1704
                     /locus_tag="PLX53_07610"
     CDS             589..>1704
                     /locus_tag="PLX53_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOY72982.1"
                     /translation="MKQINAEFGVDTANSLLKLINSHNAIDLTAGWTRFPIPVQVADL
                     VARELRQTHGRAPIEGTLELRKHVSEYIQKKYFRTYHPAAEIAITSGAAQAIFSAVAA
                     LVSDGDEVIIFEPTRNDTALAIEVCGARPVYLKLHEDNASIDWHEVQKSVTPRTKLII
                     ISSPNSLTGSILSPDDMENLQKLINGTKLKIISDETLSDIVYPGVIPSSVAFFPKLAD
                     NAYIIGSLSQTLGISDWDLGYCLAPAAMMNNFQRIQRAIANTANHALQLAIADFMSTN
                     ETSFPYHAVIQKNRQILLDSLSSTRFKPLPMQAGYTQTLDYSDVSDLKDVEFAEMLVR
                     DYGVGVSPVSIYFHDKTIRKHLLINLAVEVDVMTEAAL"
BASE COUNT          458 a          411 c          357 g          478 t
ORIGIN      
        1 cctctgagcg agcttcactt cgatcctgtt aaaatcagca tttcaatgtt tgttagcgag
       61 gtgatttacc gcaccgttag ggaggttgaa cccaactcag agctgtatga atttttggaa
      121 aattctataa ggctgctgga tgtcatgcac gatggagtgg ctaacttcca cctgattttt
      181 ctcatccagc taaccagata cctgggtttt tacccggcaa acggatggag cgaaaccatg
      241 ccgtttttcg actataaaaa tggctttttc tgtccttcac caccatccca ttctttcaac
      301 ctcgacaaaa cttcaagccg tcgcctggga atgctgctag gcaccccatt ttataggtca
      361 aatgaaatat ctctaaacca tgctcaaaga attcaggcaa tcaacgacct gttgtgctac
      421 tacaacttcc accttggctc ccctctcgat tttaaaacgc ttccaatttt aattcaactt
      481 tttcaataac gcactttcac caccaaattg gctcaatttg tcaaacattt atggtggggt
      541 ggcacaactt gagtattttt ataaaacaat gataaatttt tcttttgaat gaaacagatt
      601 aacgcggaat ttggagttga tacggcaaat tccttgctta aacttattaa tagccataat
      661 gcgatagatc taactgcggg atggacaagg tttcccatac cagtgcaggt ggccgatttg
      721 gttgccaggg agttgaggca aacgcatgga cgtgcgccca tagagggcac gctagagctc
      781 agaaagcatg tttctgagta cattcagaaa aaatactttc ggacatacca tcctgcagct
      841 gaaattgcca ttacctcggg tgctgcccag gcaatttttt cggcagttgc ggctttggtg
      901 agtgatggtg atgaggttat cattttcgag cccacacgca acgacacagc gctagccatc
      961 gaggtttgtg gtgcccgacc tgtttatctt aagctacacg aggataacgc aagtattgat
     1021 tggcatgaag ttcaaaagtc ggttaccccg cgtaccaagc tcataatcat aagctctcct
     1081 aacagcctta ccggaagcat cctttcgcct gacgacatgg agaatttgca aaagctgata
     1141 aacggaacaa agcttaaaat tatcagcgat gaaactttga gcgatatcgt gtaccctggc
     1201 gttattccat ctagcgtggc gttttttcct aaattggccg ataacgcata cataattggc
     1261 tcgttgagcc aaaccctggg aatttccgat tgggacttag gttactgcct tgctccagca
     1321 gccatgatga acaactttca acgtattcag cgagccattg ccaacaccgc caaccacgcc
     1381 ctacagctgg ctattgccga tttcatgagc accaacgaaa cttctttccc ctaccatgct
     1441 gtaattcaga aaaatcgcca gattctgctt gattctcttt catctaccag gttcaaacca
     1501 ctacccatgc aggccggata cactcaaacc ctggactact cggacgtgtc ggatttaaaa
     1561 gatgttgaat ttgccgaaat gctggttagg gattatggcg ttggggtttc gcctgtttcc
     1621 atttacttcc acgataaaac cattcgaaag cacttgctga taaacctggc tgttgaagtt
     1681 gacgtaatga ccgaggctgc tctt
//
LOCUS       DAOOQX010000479         1704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_90803_length_1704_cov_2.192238, whole genome shotgun sequence.
ACCESSION   DAOOQX010000479 DAOOQX010000000
VERSION     DAOOQX010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90803_length_1704_cov_2.192238"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1020
                     /locus_tag="PLX53_07615"
     CDS             109..1020
                     /locus_tag="PLX53_07615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOY72983.1"
                     /translation="MEEYACKKYITTVDDLKDTLKEYGVAIIPKVLNDDECKSMVSGM
                     WTFLEYITSEWETPISSEDNETWSEFYKLYPGHNMMIQHYGVGHAPISWQIRQNKKIV
                     NIFAKLWNVEAEDLLVSFDGLSFHLPSEITKKKSMNNAWYHSDQNYKPNNFECIVENF
                     SVRDFLPSDFEALSLVWSLTGLGNPARGDNLEIIMQSIAMGGKLLVATSSQGLVVGTS
                     WMTFDGRRIHLHHFGVHPSYQRRGIGKMLAAESLRFVKQKGYQVKLEVHQGNLAAINL
                     YKQFGFQRLGDYDIYIIRDLTVIDSVL"
     gene            complement(1054..>1704)
                     /locus_tag="PLX53_07620"
     CDS             complement(1054..>1704)
                     /locus_tag="PLX53_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOY72984.1"
                     /translation="RSNRNSNVVICYSNKQANRYNIAIRSRILFNEEELSVGDYLMVA
                     RNNYYWVADNPNIAFIANGEIVEVKRIGKHYNLYGFRFADVTLKLTDEDNQEVDARII
                     LDSLTIDGPSLGSEQMKNLYAAVAQDYQHIKTYRTRLQKIREDPFFNAIQVKFAYAVT
                     CHKAQGGQWPNVFIDQGFFRESMINREYLRWLYTAVTRATSKVYMVNFNDIFFGNE"
BASE COUNT          516 a          315 c          381 g          492 t
ORIGIN      
        1 tcttttcgaa gaaaaattat atatgtaaat attcaatgca ttttatataa aaaaagtgaa
       61 agtaaaactt attattaata tatcaataat attaatataa taaagacaat ggaagagtac
      121 gcgtgcaaaa agtatattac aactgttgat gatttgaaag atactttaaa agaatatggt
      181 gtagctatta taccaaaggt tttgaatgat gatgaatgca aatcgatggt ttcaggcatg
      241 tggacatttt tagaatatat aacgagtgaa tgggaaacac caattagtag tgaagataat
      301 gaaacatggt cagaatttta taaactttat cctgggcaca atatgatgat tcaacattac
      361 ggtgtaggac atgctccaat atcatggcaa attagacaaa ataaaaagat agttaatata
      421 tttgcaaagc tatggaatgt tgaagcagaa gatttacttg tatcttttga tggattaagt
      481 tttcatcttc cttcagaaat aacaaaaaag aaatcaatga acaatgcatg gtatcattct
      541 gatcaaaatt acaagccgaa taattttgaa tgtatcgtgg aaaatttctc agtaagggat
      601 tttttaccaa gcgattttga agcattatcg ctagtttgga gcctaacggg tttgggaaat
      661 cctgctcgtg gtgataacct ggaaattatc atgcagagca tagctatggg cgggaagctg
      721 ctggttgcta ccagctccca gggccttgtg gtgggtacta gctggatgac ctttgacggg
      781 cgtagaatac accttcacca cttcggcgta catcccagct accagcgaag ggggattggc
      841 aagatgctgg cagccgagtc actaaggttt gtgaagcaaa aaggctacca ggtgaagctg
      901 gaggttcatc agggtaacct ggctgcaatc aacctttaca agcagtttgg ctttcaacgt
      961 ctgggtgatt acgatatcta catcattaga gatttaaccg tgattgatag tgtgctctag
     1021 ccttacccgt tagacttagc acagcagtgc aagctactca tttccaaaga aaatgtcgtt
     1081 aaaatttacc atgtaaacct ttgaggtagc acgggtaaca gccgtgtaga gccagcggag
     1141 gtactcacgg ttgatcatgc tctccctaaa aaatccctgg tcaataaaca cgttgggcca
     1201 ctgtccaccc tgggccttat ggcatgtaac tgcataggca aacttaacct gaatagcgtt
     1261 gaagaaagga tcctccctaa ttttttgcag ccttgtccgg taggttttaa tatgctggta
     1321 atcctgtgct acggctgcgt aaaggttttt catctgctcc gagccgagcg agggtccgtc
     1381 aatggtgagt gagtcgagaa taatcctggc atctacctcc tggttatcct cgtcagtcag
     1441 cttcagggta acatcggcaa aacgaaagcc gtaaaggttg taatgtttac ctatgcgttt
     1501 aacctctaca atttcaccgt tggcaataaa agcaatgttt gggttatctg ccacccagta
     1561 gtagttgtta cgggccacca taaggtagtc gcctaccgaa agttcctcct cgttgaacag
     1621 tatccgtgaa cgtatggcta tgttgtagcg gtttgcctgc ttgttggagt aacagatgac
     1681 cacgttgctg tttctattgc tacg
//
LOCUS       DAOOQX010000480         1701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_91008_length_1701_cov_3.126974, whole genome shotgun sequence.
ACCESSION   DAOOQX010000480 DAOOQX010000000
VERSION     DAOOQX010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91008_length_1701_cov_3.126974"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            475..>1701
                     /locus_tag="PLX53_07625"
     CDS             475..>1701
                     /locus_tag="PLX53_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOY72985.1"
                     /translation="MNNAIFKYDFPKNEPILGYLPGSPERLALEAEIKKLSSEVMDIP
                     LIIGGKEVRTGKTGKVVMPHDHQHVLANYHIAGEEEVKMAIEAALKAHEYWGTVSWVE
                     RLSITVKAAELLSTKYRALINAATMLGQDKNVYQAEIDSACEAIDFLRFNAYYISEVY
                     NHQPSSDKSSINRLEYRPLEGFVFAVTPFNFTAIASNLNMSPVLMGNTVVWKPATTAI
                     LSNYILMKVYKEAGLPDGVINFIPGKGSAIGNIVLNHPMLAGIHFTGSTATFNQFWKT
                     VSNNIGHYRSYPKLIGETGGKDFMFLHSSANINEAVTAAVRGAFEFQGQKCSAASRAY
                     IPQSLWPEFKSKMTATLKEIKQGDPTDYETFINAVIDEASFDNIMQYINQAKTSDKAE
                     IIWGGKGDTSRGYFVEP"
BASE COUNT          502 a          381 c          366 g          452 t
ORIGIN      
        1 agctacagcc ttttcgccgc caccctgcag gatttgctcc ttgcgttttt ttagctcctc
       61 tactttgcta tctttttcat gcatagcttg atttgtttgg ttagtaaatt gtccacctgt
      121 aatcctcatc atacatcgaa agtgcaaggt tactaaatag cggccatata taaaagaata
      181 ttgacttctt ttttagcctg taagagtatt aacatgctga ttttattgat gttacattga
      241 tgacttgact attcagttac cgcccaagca cattaacgta accgaaatat gcaatagtac
      301 gggtgaacat aagggatata ttcttgttaa gtttttggcg agttttaagt aaaaacaact
      361 gcctcagaaa aattggtctt aacctacttt tcaacttatt cctgataatt atcatttgtt
      421 ttttagttcg atatgaatac atttgcataa cgaataaaat aactaactga agctatgaac
      481 aacgctattt ttaagtacga ttttccgaaa aatgaaccca tcctgggtta cctacctggt
      541 tcgccagaga ggctggcact tgaagcagaa ataaaaaagc tgtcatcgga ggtaatggat
      601 atacccctga taattggggg caaggaggtt cgcacgggaa aaaccggaaa ggtggtaatg
      661 ccacacgacc atcagcatgt actggccaac taccatattg ccggcgagga agaggttaag
      721 atggccattg aggctgctct aaaagctcac gagtactggg gaacggtttc gtgggttgag
      781 aggttgagca taaccgtaaa agcagccgag ctgctttcaa ccaagtacag agcgctcatc
      841 aacgctgcaa caatgcttgg gcaggataaa aacgtgtacc aggcagaaat tgactcagca
      901 tgtgaagcta ttgattttct gaggtttaat gcctactaca tttccgaggt ttacaaccac
      961 cagccctcat cggataaaag tagcatcaac aggttggaat acagaccatt ggagggcttc
     1021 gtatttgctg taaccccgtt caacttcacc gcaatagcct ccaatctgaa catgtcgcct
     1081 gtgctgatgg gcaacaccgt tgtatggaaa cccgccacaa cagccatact ctccaactac
     1141 atcctgatga aggtttacaa agaagccgga cttccggatg gtgtcatcaa ctttattccc
     1201 ggtaagggct cagcaattgg caatatagtg cttaaccacc ccatgctggc cggaattcat
     1261 ttcaccgggt caactgctac ctttaaccag ttttggaaaa ccgtaagcaa caacataggc
     1321 cactaccgct cctaccctaa gcttattgga gaaaccggcg gaaaagactt tatgttcctc
     1381 cattcaagcg caaacatcaa cgaagcggtt actgctgctg taagaggagc gtttgaattt
     1441 cagggacaaa aatgctctgc cgcttccaga gcctacatac cccaatcgct atggccggaa
     1501 tttaagagca agatgacagc cactttgaaa gagattaagc agggcgaccc caccgattat
     1561 gagacgttta taaatgcggt gattgatgaa gcttcgtttg acaacatcat gcagtacatc
     1621 aaccaggcta aaacttctga caaggccgaa atcatctggg gtggaaaagg cgacacatcg
     1681 agaggctatt ttgttgagcc t
//
LOCUS       DAOOQX010000481         1699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_91171_length_1699_cov_3.044404, whole genome shotgun sequence.
ACCESSION   DAOOQX010000481 DAOOQX010000000
VERSION     DAOOQX010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91171_length_1699_cov_3.044404"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..226)
                     /locus_tag="PLX53_07630"
     CDS             complement(5..226)
                     /locus_tag="PLX53_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HOY72986.1"
                     /translation="MSIRLKILSGFIILASLLIISGLISIYELTKLGNSVNKLLRDNY
                     LSIDYARQMSHSLEKQNSAIQFWHILCSL"
     gene            363..932
                     /locus_tag="PLX53_07635"
     CDS             363..932
                     /locus_tag="PLX53_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOY72987.1"
                     /translation="MDDKDLVGRVIESNDHKAFAQLVERYQKMVVTTCRGFVQSYADA
                     EDLAQDVFIELFESLPGFRNESKLSTWIYRIAVNKSLNFVRRRKREVLLNNLYFWNKD
                     SNSLALGQAVSPEGEQAMLDAERSKQLKLAINKLAENQRVALILSRYQELSYKEIAEV
                     MGVSVSSVESLLFRAKENLRKMLLKGKNI"
     gene            979..1437
                     /locus_tag="PLX53_07640"
     CDS             979..1437
                     /locus_tag="PLX53_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOY72988.1"
                     /translation="MKMNCKLAQQNMLNYLDGTIDKSIINAFEEHVGSCSSCRDELQL
                     LKPIYKQVEGDLGEYAPNPYFAAKVLAKLTEKREKPTTIFTQKSYILAVSLAAAAVAV
                     GLFVGIQFVSSGSSLAQPEIATILANDYSNFSDSNPYNFEVEEVDTSLNK"
     gene            1444..>1699
                     /locus_tag="PLX53_07645"
     CDS             1444..>1699
                     /locus_tag="PLX53_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72989.1"
                     /translation="MNFLTKNRILVIVVVILSLLLLSILGGLGYGYYRSKQASKLPPP
                     ANRFEHQAAFISRSLNFSPIQNEQFIRLGEGFHFKFDSLAT"
BASE COUNT          503 a          337 c          394 g          465 t
ORIGIN      
        1 tctgctacag ggaacacaat atatgccaga attgaatggc actattttgt ttttccaggc
       61 tatgcgacat ttgccgggca tagtcaatgc ttaagtagtt atcccttaaa agtttattta
      121 ccgagttgcc tagcttggtt agctcataaa tcgaaattag ccctgaaata atcagcagcg
      181 aagccaggat tataaacccc gaaagtattt ttaggcggat actcattttt ttttacaaat
      241 gtataacgta atggtggagt ttaatacgat tgggattgtt aaattcgatt ttttagtaaa
      301 agtagattgc accgcaagaa atggcaggtt gagttgtcaa aaatacatta cattcgtagc
      361 tgatggacga caaggatttg gtaggcaggg ttattgagag taacgaccac aaggcttttg
      421 cccagctggt ggagcggtac caaaaaatgg tggttacaac ctgccggggg tttgttcaat
      481 cctacgccga tgccgaggat ttagcacagg atgttttcat cgagctcttt gagtccctac
      541 ccggctttcg taacgagtcg aaactttcaa cctggatata ccgaatagcg gttaataaat
      601 cgttaaattt tgttcgccgg cgtaaacgcg aggtgctgct gaacaacctg tatttttgga
      661 ataaggatag caactcgttg gctcttggac aagccgtttc cccggaggga gagcaggcaa
      721 tgctggatgc tgaaagaagc aagcagctga agctggccat taacaagctg gccgaaaatc
      781 aacgggtagc cctaattcta agcaggtatc aggaattgtc gtataaggaa attgccgaag
      841 ttatgggagt ttctgtatct tcggttgaat cgttactgtt cagggctaaa gagaaccttc
      901 gaaaaatgtt gctcaagggg aaaaatattt gaaaatcatc gcaagaaatc atactacaac
      961 ttgtctaatt ataaaattat gaaaatgaac tgtaagctgg cacagcaaaa tatgctgaac
     1021 taccttgatg gcacaattga caagagcatt atcaacgcct tcgaggagca tgttggttca
     1081 tgcagcagct gccgggatga gctacagcta ctaaaaccaa tatacaagca agtggaagga
     1141 gatttgggcg agtatgctcc aaatccttac tttgccgcaa aagttttggc aaagctgact
     1201 gaaaagcggg agaaaccaac gaccattttt acacaaaaga gctacatact ggcagtatcg
     1261 ctagccgctg ctgctgtggc tgttggacta tttgtcggga tacaatttgt tagctcaggg
     1321 tcatcgttgg cacagcctga gattgctaca attcttgcca acgattatag taatttctcg
     1381 gatagcaatc cgtacaattt tgaggttgaa gaggttgata cctcactaaa taagtaagcc
     1441 accatgaact ttttaacgaa aaacagaata ctggtaattg tcgtagtcat tctttcactg
     1501 ctgctccttt caattctggg aggtttgggg tatggctact acaggagcaa acaagcctca
     1561 aagctacctc cgcccgccaa caggtttgag catcaggcag catttatttc ccgatcgcta
     1621 aatttttcac ccatacaaaa cgagcagttc atcaggcttg gggaaggatt tcatttcaag
     1681 tttgactcgc tggcaacac
//
LOCUS       DAOOQX010000482         1696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_91447_length_1696_cov_2.606338, whole genome shotgun sequence.
ACCESSION   DAOOQX010000482 DAOOQX010000000
VERSION     DAOOQX010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91447_length_1696_cov_2.606338"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLX53_07650"
     CDS             complement(<1..199)
                     /locus_tag="PLX53_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HOY72990.1"
                     /translation="MVKNTDICSFCGRSRKEVSLLIAGMDGFICDLCVEQASIIVSEE
                     LKKKGEFKLQKSKLMRPTEIKK"
     gene            complement(382..1062)
                     /locus_tag="PLX53_07655"
     CDS             complement(382..1062)
                     /locus_tag="PLX53_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOY72991.1"
                     /translation="MPVAVFLSAALMLGIIRFISKPDHRYVIRGIDVSAHTGSISWEK
                     LKDQRVYFAFVKASEGATLKDKNFQDNVNGARAAGIPVGAYHFFNFNRDGLTQANNFL
                     STIGRQKYNLPLVVDVEEWGNNIRRSRSQIIADLREFVSKVEKNRYQKIIIYTNEDCY
                     KLYIQDVFPDNDIWICSFNHKPKLNREWTFWQYEHNGRLKGVKGKVDFNVFRGSMLEW
                     RQYIEDRR"
     gene            complement(1073..1420)
                     /locus_tag="PLX53_07660"
     CDS             complement(1073..1420)
                     /locus_tag="PLX53_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY72992.1"
                     /translation="MAVILVNRIGWIRIFTKIKKHSKLHEKVDKVESCRVVMGNGLKM
                     THPKLHEISIYSCTGDLCFWLQNSAVRPIPNIGFSSIVNGIKCKDFPTIVARNFERLY
                     LCGISYGKEIEEA"
     gene            1395..>1696
                     /locus_tag="PLX53_07665"
     CDS             1395..>1696
                     /locus_tag="PLX53_07665"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY72993.1"
                     /translation="MRLTRITAILFFSCIALAANGQEIGMTGTKANAERVMADSLLKN
                     QQYAQALPIYSRLLEQHSRDPQIQLNLGICYLYGTRRMDLAVKFLSQASTGDVSSIV"
BASE COUNT          468 a          416 c          320 g          492 t
ORIGIN      
        1 atttctttat ctcggtgggt ctcatcagct tgctcttctg cagcttgaac tcgccctttt
       61 tcttaagttc ttcacttaca atgatgctgg cctgctcaac gcaaaggtcg catataaacc
      121 catccatccc ggcaatcagc aagcttactt cctttcggct ccttccacaa aaagagcata
      181 tatcggtgtt tttaaccatt attcctgaaa tttatttctg cgacaaaaat agcaaatttt
      241 ttgcagcagg ttgctgttaa gctgggttaa tttggtttta ccgtgttgat tacatgattg
      301 ctaccactac catgtttacc tatggtttct gatttagcat cccattaata acgaaatgga
      361 gctcgcaaaa aaagcagatg tttaccgcct atcctctatg tattgcctcc actccagcat
      421 gctcccccta aaaacgttaa aatcgacctt gccctttacc cccttaagcc tcccgttatg
      481 ctcatactgc caaaatgtcc attccctatt gagctttggc ttatggttaa acgaacaaat
      541 ccaaatatcg ttatctggaa aaacatcctg aatgtaaagc ttatagcaat cctcgttggt
      601 gtaaataata attttttgat accggttctt ttcaacttta ctaacaaatt cgcgtagatc
      661 ggcgatgatt tggcttcgcg acctgcggat gttgttgccc cactcctcca catccacaac
      721 cagcggcagg ttatactttt gcctacctat ggtagaaaga aagttatttg cctgggtgag
      781 tccatcccgg ttgaagttaa aaaaatgata agcacctaca ggaatgccgg ccgcccgggc
      841 tccattaaca ttatcctgaa aatttttatc cttcagagtg gcaccctcgg aggccttcac
      901 aaaagcaaag taaacacgtt gatcctttag cttttcccag ctgatgcttc cggtatgcgc
      961 cgaaacatca atgccccgaa taacatacct atggtcaggt ttggaaataa atcgaataat
     1021 gcccagcatc agggcagcag agaggaaaac tgctacgggc acagaccaac ttttatgctt
     1081 cttcgatttc cttaccataa gaaattccac aaaggtaaag ccgttcaaaa tttcgcgcaa
     1141 caatagtagg aaaatcttta cattttatcc cgttaacgat agaagaaaaa ccaatgttgg
     1201 gaatgggacg aacagcgcta ttttgaagcc agaaacacaa atcgcctgta cagctataga
     1261 tacttatttc gtggagcttg gggtgtgtca ttttcaaccc atttcccatt acaacccggc
     1321 agctctcaac tttgtcaact ttttcgtgta actttgaatg ttttttgatt ttcgtaaaaa
     1381 tcctaatcca gcctatgcgg ttaaccagaa taactgccat actttttttc tcctgtatag
     1441 cccttgctgc caatggtcag gaaatcggca tgacaggaac aaaggcaaat gcggagcgtg
     1501 tcatggccga ttcgcttctg aaaaaccagc agtatgcgca ggcgctaccc atttactcaa
     1561 ggttgctgga gcagcactcc cgcgatcccc aaattcaact aaacctggga atttgttacc
     1621 tctacggaac ccgccgtatg gatttggcgg taaaattcct ttcgcaagcc tcaacgggtg
     1681 atgtaagcag catagt
//
LOCUS       DAOOQX010000483         1696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_91448_length_1696_cov_2.601463, whole genome shotgun sequence.
ACCESSION   DAOOQX010000483 DAOOQX010000000
VERSION     DAOOQX010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91448_length_1696_cov_2.601463"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLX53_07670"
     CDS             <1..645
                     /locus_tag="PLX53_07670"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOY72994.1"
                     /translation="VEYDTSFNHLKNVVVDSGYSRIPVYSETFDSIRGILYVKDLIPY
                     IDREDDFHWQNLIRTPYFVPESKRINDLLSEFQLKRIHMAIVVDEYGGTCGIITLEDI
                     LEEIVGEISDESDDETPLYTVVDSSTYIFEGKILLNDFCKVIGCSDDVFDDVRGEAET
                     LAGLILEITGQFPSRNDVVTCKQFEFTVDMVDSRRIKRVKVKVHDREDEQSNSI"
     gene            623..1201
                     /gene="gldD"
                     /locus_tag="PLX53_07675"
     CDS             623..1201
                     /gene="gldD"
                     /locus_tag="PLX53_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOY72995.1"
                     /translation="MSNRIPFSLLAILFLSVMLFGCADPPVPKPRGYFRIDFPAKSYR
                     LFDSIYPFRFMYPTYSVIVPKESADSGVWLNVDFTGYKATLHLSYKNVHGDISKMIED
                     ARSLAYKHTVKADAIDEQFFSNQQEKVYGVLYDIRGNSASSVQFFVTDSTQHYLRGAL
                     YFRCVPNKDSLAPAVDFFKKDVVHLLETLSWK"
     gene            1217..>1696
                     /locus_tag="PLX53_07680"
     CDS             1217..>1696
                     /locus_tag="PLX53_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOY72996.1"
                     /translation="MPIIKEILNEGNRIVVWQFHDGEFESPALQHESISDAVAHPKRI
                     LERQAVLAILKYMGFANGYSYTPESRPFIPGSNQHISISHSGSVAVVAVNKSTPVGVD
                     VEQLDRPFDRVAKKFLSKEEQLVVNVHDKSELALIWCVKEATYKLPWAKPCSLADDMV
                     "
BASE COUNT          441 a          384 c          398 g          473 t
ORIGIN      
        1 gtggagtacg acacttcgtt caaccacctt aaaaacgtag tggtggattc gggctacagc
       61 cgcatccctg tttactccga aaccttcgat agcattcgtg gcatactcta tgttaaagac
      121 ctgatcccct acattgaccg tgaggacgac tttcactggc aaaacctgat tagaactccc
      181 tacttcgtcc ccgagagtaa aagaatcaac gacttgctat cggagtttca gctgaaacgt
      241 atccacatgg ccattgtggt tgacgagtat ggtggaacat gcggaataat cacccttgag
      301 gatattcttg aggagattgt aggcgaaatt tctgatgaat ccgacgacga aactccgctt
      361 tacacggtag ttgacagctc aacatatatt tttgagggca aaattctgct caacgacttc
      421 tgtaaggtta ttgggtgcag cgacgatgtg tttgatgacg taaggggtga ggccgagact
      481 cttgcagggc tcattcttga aattaccgga caatttcctt cccgaaacga tgtggtaacc
      541 tgcaaacagt ttgagtttac cgtggatatg gttgatagcc ggagaataaa acgtgttaag
      601 gttaaagtgc atgatagaga agatgagcaa tcgaattcca tttagcctgc tggcaatcct
      661 gtttctttct gttatgctat tcggttgtgc cgatcctccg gttccgaaac caaggggcta
      721 ttttcggatt gatttccctg caaaaagcta ccggctcttc gattctattt acccttttcg
      781 cttcatgtac cctacctatt ctgtgattgt tcctaaggaa agcgctgatt ctggtgtatg
      841 gcttaatgtt gactttacag ggtataaggc tacactccac ctgagctaca aaaacgtgca
      901 tggcgatatt tctaaaatga ttgaggatgc ccgctccctg gcctacaagc acaccgtaaa
      961 ggctgatgct attgatgagc agtttttttc aaatcagcag gaaaaggttt acggagttct
     1021 atacgatata cgcggcaact ctgccagctc cgttcaattc tttgtaaccg actccaccca
     1081 gcattacctg cgtggtgccc tttactttag gtgcgttcca aacaaggatt ctcttgctcc
     1141 agctgttgat ttctttaaga aggatgtagt tcacctgctt gaaacccttt catggaagta
     1201 gctcaaattt aagtatatgc caattattaa ggagattcta aacgagggta accgaattgt
     1261 ggtttggcag ttccacgacg gagagttcga gtctcctgcg ctacagcatg aaagcataag
     1321 cgatgccgtt gctcacccaa aacgtattct tgagcgacaa gctgttctgg ctatactaaa
     1381 atacatggga tttgccaatg gctactccta cacccccgaa tcgcgtcctt ttattcccgg
     1441 ttccaaccag catatctcca taagccattc cggcagtgtg gcggtggtgg cggtaaataa
     1501 atcaacgccg gttggggttg acgtggagca actggacaga ccattcgata gggttgcaaa
     1561 aaaattcctc tccaaagagg agcaacttgt agttaacgtt cacgataaaa gtgagttggc
     1621 gctgatttgg tgcgtaaagg aggctaccta caagcttcca tgggctaaac cttgcagcct
     1681 ggctgatgac atggtg
//
LOCUS       DAOOQX010000484         1692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_91793_length_1692_cov_2.431888, whole genome shotgun sequence.
ACCESSION   DAOOQX010000484 DAOOQX010000000
VERSION     DAOOQX010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91793_length_1692_cov_2.431888"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..1296)
                     /locus_tag="PLX53_07685"
     CDS             complement(268..1296)
                     /locus_tag="PLX53_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOY72997.1"
                     /translation="MDSNVLVSLLVILLPLSFISERITNLVKLFLPTSNFLSIGNVRY
                     RENNPLLEKKRERRIFLISLLSGELVAFSLKADLFTILNSGKFGWDEPASGFSWIIGC
                     LFTGFFLSWGSKFWHDLLGIILEAKNMRRAIIDEKNLDVQLRAFGTQETSSPSEEVAA
                     VTIKPNQPQAAKPEFVQDKPTLQRIAKLHPLLREEAKEIYLQILAQGIAVRFTDTLRT
                     FAEQDELYAKGRTKPGKIVTQAKAGQSFHNYGLALDFCLLLADGREASWDRHTDINLN
                     LRYDWDEVVALFRHYGWEWGGDWVNFKDYPHFQKTFGLSTAQLLKLYNRKEVDGEGYV
                     VIKENGFS"
BASE COUNT          479 a          394 c          340 g          479 t
ORIGIN      
        1 caactgtcct ttgtaaattc tgcttttgct gtgtaataaa gttctggtat tcgggattgg
       61 gagttttctt gcaaagctta tcgaaatctc taacaactcc tacaacccaa atagccagta
      121 gagcagcagc aatgaaaaga aatacttttc ttttgcccat tttactatga tattatggtt
      181 tcctgaagct cttttgtaaa aagtatgacg gtaacagtat gaatcggagc tgcaagtttc
      241 tacattaaga aaaattatta atgccagcta gctaaatcca ttctctttaa taacgacata
      301 cccctctcca tcaacctcct tacggttata tagcttcagc agctgggcgg ttgatagccc
      361 gaaagttttc tgaaaatggg gataatcctt aaagttaacc cagtcgcctc cccattccca
      421 tccataatgc ctgaaaagag caaccacctc atcccagtcg tatcttaaat tcaggttgat
      481 atcggtgtgc ctgtcccacg aagcctccct tccgtcagcc aacaataggc aaaaatcaag
      541 ggcaagccca tagttatgaa acgattgtcc ggctttggcc tgggtaacta tttttccggg
      601 tttggttctt cccttagcat acagctcatc ctgttcggca aaggtcctca gcgtatcggt
      661 gaagcgaacg gcaatgccct gggcgagtat ctgcaaataa atctccttgg cctcctcgcg
      721 aagcagggga tgcagcttgg caattcgctg aagtgtaggc ttatcctgca cgaattctgg
      781 tttagctgct tgtggttgat taggcttgat cgtaacagca gccacttcct cagatggact
      841 gctagtttct tgtgtaccaa aagccctaag ctgtacatca aggttcttct cgtcaataat
      901 agccctacgc atgtttttgg cctctaggat aatacccagc aaatcgtgcc agaatttgct
      961 cccccagctc agaaagaagc cggtaaacag gcagccaata atccacgaaa agcccgatgc
     1021 aggctcgtcc cacccaaatt ttccgctatt cagaatagta aaaaggtctg ccttgagtga
     1081 gaaggcaacc aattcgcccg ataaaaggct gatcaggaat attctgcgtt cacgcttttt
     1141 ctccagtagg gggttgttct ccctgtagcg tacattccca attgacaaaa agttggaggt
     1201 tggcaggaat agttttacca ggttggttat ccgctcggag ataaaactca atggaagcag
     1261 gatgaccaac aacgaaacca gaacgttgga atccatttct cataagttat tttaccgatg
     1321 gaagttacaa aattttttga atgaaaaatt tttcagctga attttttaga aaaccataaa
     1381 gttattctac cctgctatct ataggtaaaa aatattaatg tatattttgg tagaataggt
     1441 attgaatgcc cttaattacc gaccatacag cagaaaaacc atgctggtaa aaaagaatgg
     1501 gtatttttat agtagtaggc ttgtagcagt tcagacagct gcctactcac aataaaggtc
     1561 caaaaccctg ttaatccctc gtttacaaac ctggcaaaat tcctttccgg caatcatgat
     1621 gcagttgacc tccggacgga acattccctt gctaaggtag tttgccccct cgtatgcccc
     1681 tacctttccg gc
//
LOCUS       DAOOQX010000485         1691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_91847_length_1691_cov_3.709658, whole genome shotgun sequence.
ACCESSION   DAOOQX010000485 DAOOQX010000000
VERSION     DAOOQX010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91847_length_1691_cov_3.709658"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PLX53_07690"
     CDS             <1..669
                     /locus_tag="PLX53_07690"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HOY72998.1"
                     /translation="AGCSSFLYSLVTASKFIESGTHKKVVVVGADKMSSITDYTDRAT
                     CPIFGDGAGAVLLEPNYDGLGLLDAKMQVDGSGRKHLHQVAGGSCYPASFETVAKRQH
                     YIYQEGQPVFKAAVSNMADVSVEIMERNNIKPENLAWLVPHQANMRIIEATAHRMGIS
                     KDQVMINIQRYGNTTSATIPLCLWEWESQLKKGDNIIIATFGAGFTWGAAYIKWAYDG
                     NSKH"
     gene            complement(877..>1691)
                     /locus_tag="PLX53_07695"
     CDS             complement(877..>1691)
                     /locus_tag="PLX53_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOY72999.1"
                     /translation="YDRRKFMEKNILPNEGLVGRCYVEGKTILMTDVPKNYIKITSGL
                     GGENPRCLLIVPLKVNDTVLGVIELASFNPYQKYQVDFIEKIAESIAATLSSVRVNIR
                     TAELLERTQQQAEEMKAQEEEMRQNMEELHATQEEMERKRLEQESINTQLAEDRTILN
                     SLMQNLPDCMFQKDVNGRYVRLSNSMLNLLGIDQPDEVIGLTDFDLFPTSLAKLLSKL
                     DSEVLSSRQPITSKPIELVLKNGVEQDALLTLMPLVEENGDAIGVMGVMKLA"
BASE COUNT          397 a          404 c          412 g          478 t
ORIGIN      
        1 gccggatgct ccagcttttt atattcgttg gttactgctt ctaaattcat agaatccgga
       61 acgcacaaaa aggtggttgt ggttggagcc gataagatgt cgtccattac cgactatacc
      121 gaccgggcta catgtccgat ttttggcgac ggggccggag ctgtgctgct tgaacccaac
      181 tacgacgggt tagggctact agatgccaag atgcaggttg acgggtctgg ccgtaagcac
      241 ctgcaccagg tggctggagg ttcatgctat cctgcttcat ttgaaaccgt tgcaaaacgt
      301 cagcactaca tttatcagga gggtcaaccg gtttttaaag ctgcagtttc caacatggca
      361 gatgtgtcgg ttgagattat ggaacgaaac aacattaagc cggagaacct ggcatggctg
      421 gttccacacc aggccaacat gcgtataata gaggctacag ctcatagaat gggcatcagt
      481 aaggatcagg tgatgatcaa cattcagcgc tacgggaata ctaccagtgc aaccattccc
      541 ctctgcctct gggagtggga atcgcagctg aaaaagggcg ataacatcat aattgccacg
      601 tttggcgctg gttttacatg gggtgctgct tacattaaat gggcatacga cggtaactcc
      661 aagcactagt agctagtaac atccttagtt tttttattcg aagtttggtt gaagcagaaa
      721 cccttgtcgc taagaagacg tgggaggttg tctatttaat ctactctgcc aggcaggtaa
      781 gcaatttcct tgatgcctct ttttgtgtat gaggtgttca cctagcttta caggtgctgg
      841 agttgatgct caattcctcg agccgcttta ttccgcttaa gcaagtttca tgacgcccat
      901 aactccaata gcatctccat tctcttcaac caggggcata agcgtgagta gggcatcctg
      961 ttctactcca ttcttaagca ccagctcaat gggtttactg gtaataggtt gacgagagct
     1021 cagcacttcg ctgtccagct tggaaagtag cttcgccagc gaagtaggaa acaggtcaaa
     1081 atcagttaac cctataacct cgtcgggctg gtcgattcct aaaaggttga gcatggagtt
     1141 gcttagccta acgtagcggc cgttaacatc cttttggaac atgcagtcgg gtaaattctg
     1201 catcaggctg ttgaggattg ttctatcctc agcaagctgg gtattaatgg attcctgctc
     1261 aagtcgttta cgctccatct cctcctgggt ggcgtgtagc tcctccatgt tttgcctcat
     1321 ttcctcctct tgcgctttca tttcctctgc ctgctgttgt gtacgttcca gcagctcggc
     1381 ggtacggatg ttcaccctaa cactcgacag cgtggcagct atgctttcgg ctattttttc
     1441 gataaaatca acctggtact tctgataagg gttgaatgag gccaactcta taacccccaa
     1501 caccgtgtcg tttaccttta aggggacaat taggaggcat cgggggttct cgccgcccag
     1561 ccctgaggtt attttaatgt agttcttggg aacatcggtc atcaaaattg tttttccctc
     1621 aacgtagcac ctgcccacca atccctcgtt gggcagaatg tttttttcca taaattttct
     1681 acggtcgtag g
//
LOCUS       DAOOQX010000486         1684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_92479_length_1684_cov_2.476366, whole genome shotgun sequence.
ACCESSION   DAOOQX010000486 DAOOQX010000000
VERSION     DAOOQX010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92479_length_1684_cov_2.476366"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PLX53_07700"
     CDS             <1..507
                     /locus_tag="PLX53_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOY73000.1"
                     /translation="YISPVVGILFVLGLISAAYSSADSALTALTTSFTIDILNTKDMD
                     EATLQKVRKRVHLMISAVVVAVILIFNAFNDRSVIDSIFTVAGYTYGPLLGLYAFGMF
                     TRFQTNDKLVPLIALLSPIICLLLSTYSKELFGGYEFGFELVILNGLLTFTGLYFTRR
                     KGVKIPKV"
     gene            664..>1684
                     /locus_tag="PLX53_07705"
     CDS             664..>1684
                     /locus_tag="PLX53_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOY73001.1"
                     /translation="MVETPLMKQYLSIKQKHPDAVLLFRVGDFYETFGEDAILASGVL
                     GITLTRRANGAASFVELAGFPHHALDTYLPKLVRAGYKVAICDQLEDPKLAKTIVKRG
                     ITELVTPGVAYNETILEHKENNFLGCIYLDKKLAGISFLDISTGEFLTSEGNMDYIDK
                     LLSNFKPKEVLVERSKLREFTEHFGDKYYTTRLDEWAFSEDAAREKLLNHFKTTSLKG
                     FGIDSLKLGVISAGAILYYLDITQHSQAEHISSISRIDEENYVWVDRFTSRNLELFTS
                     INEGAKTFIEVIDKTASPMGGRLLKRWVSLPLKNLNAINDRLNMVDYFFNNPDLRSTL
                     LDRIRG"
BASE COUNT          466 a          400 c          378 g          440 t
ORIGIN      
        1 tacatttcac cggttgttgg aattctcttt gtgctggggt tgatttctgc tgcctactcc
       61 agcgccgact cagcccttac tgctcttacc acctcgttca ctatagacat cctcaacaca
      121 aaggacatgg atgaggctac acttcaaaag gtgcggaaac gggttcattt aatgatatct
      181 gctgttgttg tagccgtaat tcttatcttc aacgcattta atgaccgaag tgtaattgac
      241 tccattttta ccgtagctgg ttacacctac ggcccacttc ttggactata cgcttttggc
      301 atgttcaccc gttttcaaac aaacgataag ctggttccgc taatagccct gctgtcgccc
      361 atcatctgtt tattgctaag cacatactcc aaggaacttt ttggcgggta cgagttcgga
      421 tttgagctgg taatcctgaa cggcctccta acctttacgg ggctttactt cacacgccga
      481 aaaggtgtaa aaattccgaa ggtatagccc tttttcatcc catagcccct actcatttaa
      541 tcaggctaaa actgctaatc cggtttacca aaatgttaca ttttaagctg aaacttggtg
      601 aggatttgat aattttgtgg gtaaacaaaa gcaaacacct tggacgaagg caggaataag
      661 ggcatggtgg aaacacccct gatgaagcag tacctgagca ttaagcagaa acatcccgat
      721 gcagtgctac tctttagggt gggcgatttc tacgagacct ttggagagga tgccatcctg
      781 gcatcaggcg ttctcggtat aaccctcacc cgaagggcca acggagcagc ttcttttgtg
      841 gagctggcag gatttccgca ccatgccctc gatacctacc tcccaaaact cgttagggca
      901 ggctataagg ttgccatttg cgaccagctg gaagacccaa aactggccaa aaccattgtg
      961 aaaaggggca tcaccgagct ggtaacgccc ggtgtagcat acaacgaaac catacttgag
     1021 cataaggaaa acaactttct gggttgcata tacctggata agaagttggc tggcatctcg
     1081 ttcctcgaca tatcaacagg tgaattccta acctccgagg gcaacatgga ttacattgat
     1141 aagctgctaa gcaactttaa acccaaagag gtgctggttg aacgctcgaa actgcgcgag
     1201 tttaccgaac atttcggcga taagtattac accacccggc tcgatgaatg ggcgtttagc
     1261 gaggatgccg ctcgcgaaaa actccttaac cactttaaaa caacctcgct caagggattc
     1321 ggaattgata gcttaaaact gggagtaatc tcggcgggag ccatactcta ctaccttgac
     1381 atcacgcaac acagccaagc tgagcatatt tcaagcattt cgagaattga tgaggaaaac
     1441 tacgtttggg ttgaccgctt tacttcaaga aatctggagc tgttcacctc cattaacgag
     1501 ggggctaaaa cattcatcga ggttattgat aaaaccgcat cgccaatggg tggtaggtta
     1561 ttaaaacgat gggtttcgct acccttgaag aatttgaatg ccattaatga caggctcaac
     1621 atggtggatt acttcttcaa taaccccgat ttaagaagta cactgctgga tagaataagg
     1681 ggta
//
LOCUS       DAOOQX010000487         1683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_92544_length_1683_cov_3.076781, whole genome shotgun sequence.
ACCESSION   DAOOQX010000487 DAOOQX010000000
VERSION     DAOOQX010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92544_length_1683_cov_3.076781"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..570
                     /locus_tag="PLX53_07710"
     CDS             226..570
                     /locus_tag="PLX53_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73002.1"
                     /translation="MRIGELVKHIDGKVLCCEECLDSEVVYAFASDLMSDVLTVKCEN
                     FLLITGLSNIQAVRTAEMSDIQVIVFVRNKRVSEDMIRLARENSIVLVECSCTMFKVS
                     GILYGLGIKAVY"
     gene            576..995
                     /locus_tag="PLX53_07715"
     CDS             576..995
                     /locus_tag="PLX53_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY73003.1"
                     /translation="MKFEYKVQGGDFVRAGHASSEVKKILKQLNVEPSLVKRVVVALY
                     EAEVNIVAHAYNGMAYVDIDASRVLVRLDDNGPGISDIELAMQPGFSTASSTVREMGF
                     GAGMGLPNIKSNSDEFSISSEVGKGTHLEFVVYLNSK"
     gene            1083..>1683
                     /locus_tag="PLX53_07720"
     CDS             1083..>1683
                     /locus_tag="PLX53_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOY73004.1"
                     /translation="MRVCPTEALRVRGGKALLQSNRCIDCGECYRVCPTGAIIVEQDD
                     FNSIFNYKYRVALVPSVLLAQYPEDVSVDMAIHALLRIGFTKVVIVEDSVDYLITRIN
                     AYIINNPSRKPIISSFCPAIVRLVQVRFPWLVGNIMLVKPPIDLTSSFIRRKLTEEGI
                     PEQEVGIFYITPCAAKIAAVKSPEGEDVSEINGVINMDLI"
BASE COUNT          493 a          278 c          417 g          495 t
ORIGIN      
        1 gaactcgcaa ttagcaaccg gttgttcata cgcccttgta caatttgccc agcatgagca
       61 acaaaagtag gagaatgagt aaaaatggat ttttcatcag ccgttagtca atgggtataa
      121 tcgttagcca aatagcgtat gtattataca tgtttaataa tacatgttat attaataaca
      181 ataaatatat ttgtggcgta taattattgt agtaaaaaat attttatgcg gataggtgag
      241 ctggtaaagc atatagatgg aaaggtgcta tgctgtgagg agtgcctcga cagcgaggta
      301 gtttatgctt ttgcctcgga tctgatgagc gatgtgctaa ctgttaaatg tgagaatttt
      361 ttgctgatta cagggttgtc caacattcaa gctgtacgca ccgccgagat gtccgatatt
      421 caagttatcg tatttgttcg gaacaagcgg gtgagcgagg atatgattag gttggcaagg
      481 gaaaatagca tagttttggt ggagtgcagc tgcaccatgt ttaaggtttc tggaatttta
      541 tacggcttgg gcatcaaagc agtttactga taacgatgaa atttgaatat aaagtacagg
      601 gtggtgattt tgtgagagcc ggccacgcat caagtgaggt taaaaaaatt ttaaaacagc
      661 tgaatgtaga accttcactg gtgaaacggg tggtggtggc actttatgag gcagaggtta
      721 atattgtagc tcatgcgtat aacggaatgg catatgttga tattgatgca agtagggtgc
      781 tggttaggct tgatgataat ggcccaggaa tttcggatat tgagctggcc atgcagcccg
      841 gtttttcaac cgcatcttca accgtaaggg agatgggctt tggagcagga atggggttgc
      901 caaatattaa aagtaattcc gatgaattta gcatttccag cgaggtgggg aaaggaactc
      961 atttggaatt tgtagtttac ctaaacagta aataacgtga gaggaggtta gagttggaaa
     1021 cttctacttt tcaccacgct ttaaaaattc aatccgatgt ctgcattggc tgtgcacatt
     1081 gtatgagggt atgcccaacc gaggctctta gagtaagagg aggtaaagcc ttacttcaaa
     1141 gcaacaggtg tattgattgt ggtgaatgct acagggtttg tccaaccgga gcaattattg
     1201 tagaacagga tgactttaat agcattttca actacaagta tagggtggct cttgtaccat
     1261 cagtgcttct tgcccagtat cccgaagatg tttcggttga tatggccatc cacgctctac
     1321 taaggatagg atttactaag gtagtaattg ttgaagactc cgtcgattac ctaattacca
     1381 ggataaacgc ttatattata aacaacccta gccgaaagcc cataatctct tctttttgcc
     1441 cggcaattgt caggttggtt caggtccgat ttccctggct tgtaggaaac attatgttgg
     1501 taaagccccc aatagatctt acttcatcat tcataagaag gaagcttacg gaggaaggaa
     1561 ttcctgaaca ggaggttgga attttttaca ttactccatg tgcggccaaa attgccgcag
     1621 ttaaaagccc cgaaggggag gatgtatccg aaataaatgg ggttattaac atggatttaa
     1681 ttt
//
LOCUS       DAOOQX010000488         1683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_92587_length_1683_cov_1.708231, whole genome shotgun sequence.
ACCESSION   DAOOQX010000488 DAOOQX010000000
VERSION     DAOOQX010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92587_length_1683_cov_1.708231"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PLX53_07725"
     CDS             complement(<1..964)
                     /locus_tag="PLX53_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73005.1"
                     /translation="MTRRILILVATILGFTVGLGAQNSQGKTDDLGRIALAAIVPEQA
                     EGIPQSARQQLVNKMEQIAVQNGLGAAAANPRFCIVPMVNVLSKEITPTAPPMQAVNL
                     EVTFYIVDAASQNIFSQTSIQVKGVGQTEDKAYIQALKGVNVKAGQFKGFVEKGKEKI
                     IEYYNSQCDVVLKGAQALAGQKKYEEALYILLSVPDVCMECFDKSMDLSVEIYKQYAN
                     YKCTEYMAGAKAAWANMNVDKAAEYLGKITPDMECYSEAVKLADEITQKQLADGANVW
                     DFKMKQYDDSIEKDKMMIQAGKEVAQSWAYWGAASYFNWDWSWLY"
     gene            complement(993..>1683)
                     /locus_tag="PLX53_07730"
     CDS             complement(993..>1683)
                     /locus_tag="PLX53_07730"
                     /inference="COORDINATES: protein motif:HMM:NF040342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HOY73006.1"
                     /translation="MKNLEQERAESSMLTSSSSGSEMAESPIDALKRVAKADIIMDLD
                     FEVKRQGPQKYIVFNLKGLDAYTAKQVSGVAGAGNPSSAASPELLLEEAVLSHMDNFN
                     AGLMRHFEDMFANGREVKVQIKVWSNWGENLESEFGDNSEELSTIIENWFADNCVQGR
                     FSLSDATENFMKFEQVRIPMMRTDDKGRERAVDTRSFVSDLRKYLNGMGIPSKLYLRG
                     LGEAWLILGEK"
BASE COUNT          365 a          469 c          384 g          465 t
ORIGIN      
        1 cgtaaagcca actccaatcc cagttgaagt aggaggcagc gccccagtat gcccacgact
       61 gggctacctc cttgcctgcc tgtatcatca tcttgtcctt ctcaatggaa tcgtcgtact
      121 gcttcatctt gaaatcccaa acattagccc cgtcggcaag ctgcttctgg gttatctcat
      181 cggccagctt cactgcttcg ctgtagcact ccatatcggg ggtaattttc cccaggtact
      241 cggcagcctt gtcaacgttc atgttggccc acgcagcttt tgctccagcc atgtactccg
      301 tgcacttgta gttggcgtac tgcttgtata tctctacgga taaatccatg ctcttgtcga
      361 agcactccat gcaaacatcc ggaacggaga gcaggatgta gagggcctcc tcatatttct
      421 tctgaccggc aagggcctgg gcgcctttca gcaccacgtc gcactgcgag ttgtagtact
      481 ctattatctt ttcctttccc ttttcaacaa aacccttgaa ctgccctgct tttacgttga
      541 ctcccttcag cgcttgaatg taggccttat cctccgtctg cccaacaccc ttaacctgaa
      601 tgctggtctg gctgaagatg ttctggcttg cagcgtcaac aatgtagaag gttacttcaa
      661 ggtttacagc ctgcattggc ggtgctgttg gggttatctc cttgcttaac acgtttacca
      721 taggcacaat gcagaaccgg gggtttgcag ccgctgcgcc cagcccattc tgcacggcta
      781 tttgctccat cttatttacc agctgctggc gagccgattg gggaatgccc tcggcctgct
      841 cgggaactat cgctgcaagg gcaatgcgcc ctaagtcgtc ggttttaccc tgactatttt
      901 gggcacccaa tccaacggta aatccaagga tggtagcaac aagaatcaat attcgtctag
      961 tcatcacctt ttattttctg attaatagca ttttactttt caccaaggat tagccaggcc
     1021 tctcctagcc ctctcaggta taactttgaa ggaatgccca ttccgttgag gtacttgcgc
     1081 aagtcgctta cgaacgaacg ggtgtcaacc gcacgctccc gacccttatc gtcagtacgc
     1141 atcatgggaa tgcgcacctg ctcaaacttc atgaaatttt cggtagcatc ggaaaggctg
     1201 aagcggccct gaacgcagtt gtcggcaaac cagttctcga tgatggttga aagctcctcg
     1261 gagttgtcgc caaattccga ttcgaggttt tcaccccagt tgctccatac cttaatctga
     1321 accttcacct cgcgaccatt tgcaaacata tcctcaaaat gcctcatcag acctgcatta
     1381 aagttgtcca tgtggctgag aacagcttcc tccaggagaa gctcgggtga ggcagccgaa
     1441 gatgggttac cggcacctgc tacgcccgat acctgtttgg cggtgtaggc atcaaggcct
     1501 ttaaggttga aaacgatgta cttttggggt ccctgccttt tcacctcgaa gtcgaggtcc
     1561 atgatgatgt cggcttttgc aacacgcttt aatgcgtcaa tagggctttc ggccatttcg
     1621 gagcctgaag atgaactggt aagcatggat gattctgctc tttcttgttc caaattcttc
     1681 atc
//
LOCUS       DAOOQX010000489         1666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_94166_length_1666_cov_2.642458, whole genome shotgun sequence.
ACCESSION   DAOOQX010000489 DAOOQX010000000
VERSION     DAOOQX010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94166_length_1666_cov_2.642458"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1456
                     /locus_tag="PLX53_07735"
     CDS             245..1456
                     /locus_tag="PLX53_07735"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOY73007.1"
                     /translation="MIILVLNCGSSSIKYQLINMANESQLLAKGIVERVGFKDAILTH
                     KPVGKDKYEMVASIPDHTTGINLILEALVDKNHGVVESLSQIKAVGHRVAHGGEFFTK
                     SCLIDSNTKREIEKCIELAPLHNPANLKGILSMEKLLPNVPQVAVFDTSFHQTMPKEA
                     YLYALPYEYYENYKIRRYGFHGTSHMFVAKKACKILGADFDQLRIITCHLGNGASIAA
                     IKNGKSIDTSMGFTPVEGLIMGTRSGDIDPGILLFIAEKENLSIQQVNDVINKKSGVF
                     GISGISSDMRDLEDAAASGNERARLALDMYYYRVKKYIGSYAAAMDGVDLIIFTGGIG
                     ENDSTLREKVCGALDFMGVEFDVEANRGVRGQDKLLTKPNSLVKVMAITTNEELVIAT
                     DTANIINNLKN"
BASE COUNT          503 a          296 c          371 g          496 t
ORIGIN      
        1 tttttgccta tacgattttt attcaacata ttttattggt cattttagca tgttgtagaa
       61 cattttactg ttcttgtgtc gcaaatagcc atgttacagc attttttaaa cagtagctca
      121 gctatttgta ggtcgtaagg tttcttaaat tacttaaaac acgttgaaat ccataagttt
      181 tatttctaat tttgcatagt tttattacaa atttcaacta actgattacc aaataataac
      241 aaaaatgatt attttagttt tgaactgcgg aagttcgtca atcaagtacc agctgattaa
      301 catggctaac gaaagccagc tgttagctaa gggtattgta gaacgtgttg gttttaagga
      361 tgccattctt acccacaaac cagttggaaa ggataagtat gaaatggtgg cttccattcc
      421 ggatcataca accggtatta atctcatttt agaggcactt gttgacaaaa atcatggagt
      481 ggtagaaagc ttaagtcaga taaaagcggt tggccacagg gtggctcatg gtggtgagtt
      541 ttttaccaag agctgcctga tagatagtaa cacgaaaagg gagatagaga agtgtattga
      601 gttggctccc ctgcacaacc ctgcaaactt aaagggtatt ctttcaatgg agaagctttt
      661 gccaaatgtg cctcaggttg ctgtgtttga tacatccttt caccaaacca tgcccaagga
      721 agcctacctt tacgctcttc cctacgagta ctatgaaaac tataaaattc gcaggtatgg
      781 atttcatggc accagccaca tgtttgttgc aaaaaaagct tgtaaaattt taggagccga
      841 ctttgaccag ctacgaatta ttacatgcca tttggggaat ggtgcttcaa ttgcagccat
      901 caagaatggc aaatctattg atacctcaat gggatttact cctgtggaag gcttaataat
      961 ggggacacga agcggcgata ttgatccggg catcttgctg tttattgccg aaaaggaaaa
     1021 tctttccatt cagcaggtta atgatgtaat caataaaaag agcggcgttt ttggaatttc
     1081 cggaatatcc tccgacatgc gcgaccttga ggatgcggct gcaagcggaa acgaacgtgc
     1141 acgcctggcg ctggatatgt actactacag ggtaaaaaaa tacataggct cgtatgcagc
     1201 agcaatggat ggtgttgatt taatcatctt tacgggagga attggtgaaa atgacagcac
     1261 tcttcgcgaa aaggtatgcg gtgcccttga ttttatgggg gttgaatttg atgtagaggc
     1321 aaataggggc gttcgtggac aggataagct acttacaaaa cctaactccc ttgtaaaggt
     1381 tatggctatt acaaccaacg aagagcttgt gattgcaact gatactgcaa atattatcaa
     1441 taacttgaaa aactagaaca attaatttta actatagagg agattaggaa tatgagagta
     1501 gataagcttg accaactttt cgatttggta aaatcaaagc cccgaagaag acttgttgct
     1561 gcatttgcca acgatgccca cactattgag gcagttagcc tggctgttga taggggtatt
     1621 gttgatgcca ctcttgtttg cgatgagcct gttatgaaaa aggtgt
//
LOCUS       DAOOQX010000490         1665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_94263_length_1665_cov_2.749689, whole genome shotgun sequence.
ACCESSION   DAOOQX010000490 DAOOQX010000000
VERSION     DAOOQX010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94263_length_1665_cov_2.749689"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(337..1326)
                     /locus_tag="PLX53_07740"
     CDS             complement(337..1326)
                     /locus_tag="PLX53_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOY73008.1"
                     /translation="MVNSWFVIVNPRAGGGKGEVDWPLIKGLLTKEGIAFGFALTEHK
                     HHAVELTVKAINSGYRQLIAVGGDGTLNEVVNGIFIQQSVPPAEILVGVIGVGTGNDW
                     FRMYEIPYRYADSVRAIKNRKVFYQDVGVVEYYESSVKHKRYFVNTAGIGFDAEVALR
                     TNRLKDLGRHGFLLYLISILRALFSYKTTRINVNVDGRHLQDKIFSITLGICRYNGAG
                     MLQVPFAISDDGMLDVTVIRRISKLDALTSIPRLYNGKILKHSRVTGLRGQIVEIMSI
                     PSTNLEADGESLGETPLRFTVLTRAIGVVVDENFDAKDNRPAVIQEKQTLSAV"
     gene            1574..>1665
                     /locus_tag="PLX53_07745"
     CDS             1574..>1665
                     /locus_tag="PLX53_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOY73009.1"
                     /translation="MGKVIAIANQKGGVGKTTSAINLSASLAVL"
BASE COUNT          450 a          364 c          384 g          467 t
ORIGIN      
        1 atgaaacact tctggaacat ggattatgat ttttcctcgt aaaagtaaac aaatcaaatt
       61 aatatgaaac ctccatgctt caaaagcaca gacgtaacca aagtaaaacc tgttacgagg
      121 aggttccaac ataccgttca tttagatgtt aatgatgatt tttttgagct ttgatcttaa
      181 tggttacccc ggcatgtttt ttcggtatga tgcctgctgg taagaaacga atctaggggg
      241 ttgtctagta ttggctgcta tataaatgtt gaaaaagtaa tgcccagggt agatgtgcgg
      301 gtgaatggaa actctaataa gcgggaagca ggatgattag actgccgaga gggtttgttt
      361 ctcctgaata acagcagggc ggttatcctt agcatcaaag ttttcgtcaa ccaccacgcc
      421 tattgcacgg gttaggacgg tgaagcgcag aggggtttcg cctagcgatt cgccatctgc
      481 ctcaagatta gttgagggaa tgctcataat ttcaactatt tggccacgca gtccggttac
      541 cctggagtgt tttaagattt tcccgttgta gagccgggga atgctggtta gagcatccag
      601 cttgctgatt ctgcggataa cggtaacgtc aagcattcca tcatcggaga tggcaaaggg
      661 aacctgtagc ataccggcgc cgttgtaacg gcaaatcccc agggtaatgc tgaaaatttt
      721 atcctgtaaa tggcggccat ccacatttac gtttatccgg gttgttttgt agctaaaaag
      781 agctcgaagg atgctgatga ggtagagcaa aaaaccatgt cttcccaaat cctttaacct
      841 gttggttcgg agcgccactt cagcatcaaa gcctattccg gcggtgttaa caaagtatcg
      901 cttatgcttt acgctagact catagtactc caccacacct acgtcctggt agaaaacctt
      961 acgatttttt attgctctta cgctatcggc gtacctgtat ggaatttcgt acattcgaaa
     1021 ccagtcattg ccggttccta cgccaattac tccaaccaga atctcggcag gtggaaccga
     1081 ctgctggatg aaaatgccgt ttaccacctc gttgagcgtt ccatcacccc caacggcaat
     1141 cagctgcctg tagccgctat tgatggcttt caccgtaagc tccacagcat ggtgcttatg
     1201 ttcggtaaga gcaaatccaa aagctatccc ctccttagtc aacaaaccct tgatgagcgg
     1261 ccagtcaacc tcacccttac ccccacctgc ccgggggttt acaataacaa accagctgtt
     1321 taccaatcag atagatttta gcatcgaatt ccagctgcaa agatagcaca tagaagttta
     1381 attgtatggc ttaaggcagg gtcaaccttt tttttgaaaa atttctatct ttgccggtaa
     1441 gtatgattaa atcggttgtt gaaaacatgt ttatatcctg ttgaatacta ttcatattcc
     1501 ccagcggtgg gggcaggatg gttgcgggag gttttgtatt tttgacgctt gttttaatgt
     1561 taaagattag gtaatgggaa aagttattgc aatagccaac cagaagggtg gggtgggtaa
     1621 aaccaccagc gctatcaatt tgtcggcaag tctggcagtg ctcga
//
LOCUS       DAOOQX010000491         1663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_94476_length_1663_cov_2.416045, whole genome shotgun sequence.
ACCESSION   DAOOQX010000491 DAOOQX010000000
VERSION     DAOOQX010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94476_length_1663_cov_2.416045"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1663
                     /locus_tag="PLX53_07750"
     CDS             <1..>1663
                     /locus_tag="PLX53_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOY73010.1"
                     /translation="IANGNSAFFEGADASGANSFVAIRPIIIGSCPQSWALVLLVPKG
                     VVLHDATVIFWKTLLVGLAGILLLTLLIQAVARKYILEPIIHVSENLSRLAKGHVDGS
                     MKVHVEVSDELGAMSEYLNRTVDGLTKKTEFAQTIGQGNYGAKMELMSDEDALGQSLI
                     DLSANLKKAQEEVQKRAADDQKRQWTNEGLAKFSEILRQNNDRLDLLSINILRNLIDY
                     LGANQGGLFVYNDDDPSNPFFDLLAAYAYNRQKFIKKRILPGEGLIGTCAIEKQTIYL
                     TEIPENYITISSGLGEAKPRNLLIVPMKVDENVLGVIEIASFSAIMDYQVEFVEKLAQ
                     SIAQTLTTVKTNLKTAELLEKTQQQTEEMKAQEEEVRQNLEELATIKEELEKRNEDIL
                     ENQKQLEWEKSLLDSLLNYVPDKIYFKDLKSRFIKVSRSTLKAHGFENPDDLIGKTDF
                     DLFGEEHARPAYEDEQNIIRTDSPIIGLVEKEVFTDGRVSWAETSKLPLKNPRGETIG
                     TFGITRDITASKLMEEEVKKKEMDAVAYQRDMQKKALEAQNLYEA"
BASE COUNT          495 a          395 c          415 g          358 t
ORIGIN      
        1 ttattgccaa cggcaactct gccttttttg agggtgccga tgctagtgga gcaaattcat
       61 ttgtggccat tcgtccaatc ataattggta gttgccccca gagctgggcg ttggtgttgc
      121 ttgttccaaa aggtgttgtg ctgcatgatg ccaccgtaat tttttggaaa accctgctgg
      181 ttggcctggc cggcattctg ttgctcacct tgctgataca ggcagtagcc aggaaataca
      241 ttctggagcc tattattcac gtttctgaaa accttagccg gctggcaaaa ggacatgtgg
      301 atggcagcat gaaggtgcat gttgaggtta gcgatgagct gggggccatg tcggaatatt
      361 tgaatagaac agttgacggt ctcaccaaaa aaactgagtt tgcccaaact atcgggcagg
      421 gtaactatgg agctaaaatg gagctgatga gcgatgagga tgcccttggc caatcgctaa
      481 tagacctatc ggctaacctt aaaaaggcgc aggaggaagt tcaaaaacga gcagctgatg
      541 accaaaagcg gcaatggact aatgaggggc tggcaaaatt cagtgaaatt ctccggcaga
      601 ataacgatag gctcgacctg ctgtccatca acatccttcg taacctgatt gactaccttg
      661 gggctaacca gggtggcctg tttgtttaca acgacgacga cccgtcaaac ccattcttcg
      721 acctactggc tgcctatgct tacaaccgtc aaaagttcat taagaaacgg atactccctg
      781 gcgagggttt aatcggaaca tgtgccattg agaagcaaac catttacctc accgaaatac
      841 cagaaaacta catcaccatt tcctccggac ttggagaagc caaaccccgc aacctcctta
      901 ttgtgcccat gaaggttgac gaaaacgttc tgggagttat agaaatagcc tctttctcag
      961 ccataatgga ctaccaggta gagtttgtag aaaagttagc tcaaagcata gcccaaaccc
     1021 ttaccaccgt gaagacaaac cttaaaaccg ccgagctgct cgaaaaaacc cagcaacaaa
     1081 cagaggagat gaaggctcag gaggaggagg taagacagaa cctggaggaa cttgccacca
     1141 taaaggagga actcgaaaaa cgcaacgagg acattcttga aaaccagaaa cagctcgagt
     1201 gggaaaaatc gctactcgac tcgctgctaa actacgtccc cgacaaaata tacttcaaag
     1261 acctgaagag ccgcttcatc aaggtaagca ggtcaaccct aaaagcccac gggttcgaga
     1321 acccggatga cctgattggc aaaaccgact tcgacctttt tggagaggag catgcgcgtc
     1381 ccgcctacga ggacgagcag aacattatac gaaccgactc tcctattatt gggctggtgg
     1441 agaaggaggt gtttaccgac ggcagggtat cgtgggccga aacttccaag ctgcctctta
     1501 agaatccaag gggagaaact attggcacct tcggaattac tcgcgatatt accgcatcga
     1561 agctcatgga ggaagaggtt aagaaaaagg aaatggatgc cgttgcatac cagcgcgaca
     1621 tgcagaaaaa agccctagag gcgcaaaacc tctacgaggc aat
//
LOCUS       DAOOQX010000492         1655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_95169_length_1655_cov_3.582500, whole genome shotgun sequence.
ACCESSION   DAOOQX010000492 DAOOQX010000000
VERSION     DAOOQX010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95169_length_1655_cov_3.582500"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLX53_07755"
     CDS             <1..305
                     /locus_tag="PLX53_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOY73011.1"
                     /translation="EGTRSADGTIHRFKDGAFILAQKSEKPILPVVIDGSGEIMQNPL
                     KLKLAQTLTIKILPEISYESFREFSPNELARHVQQLMEKEHMAIAPERYHGSMVNS"
     gene            433..744
                     /gene="rplU"
                     /locus_tag="PLX53_07760"
     CDS             433..744
                     /gene="rplU"
                     /locus_tag="PLX53_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOY73012.1"
                     /translation="MYAIVDIVGQQFKVEKDQKLFVNRMAGETGTEVKFDKVLLVDND
                     GAVTVGKPQVENASVTAKILRHVRGDKVIVFKKKRRTGYKKKNGHRQNYTEIQISEIV
                     A"
     gene            765..1025
                     /gene="rpmA"
                     /locus_tag="PLX53_07765"
     CDS             765..1025
                     /gene="rpmA"
                     /locus_tag="PLX53_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOY73013.1"
                     /translation="MAHKKGVGSSRNGRESHSKRLGVKLFGGQVAKAGNIIVRQRGTK
                     HNPGQNVGMGRDHTLYALVDGEVSFRKKTDNRSYVSVIPKEA"
     gene            1115..>1655
                     /locus_tag="PLX53_07770"
     CDS             1115..>1655
                     /locus_tag="PLX53_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOY73014.1"
                     /translation="MLTLKQIRENKEEVIRRLAVKHFDGAAIVGKIIELDDRRKAIQL
                     QLDTNLAEQNSLARQIGKLFAEGKSVEANDAKARTTNLKEQSKLLTQQLAGVEAEQRQ
                     LLLQIPNLPHDSVPKGVGADDNVIVRSGGEIPELPGDVLPHWDLAKKYDIIDFELGVK
                     ITGSGFPVYKGKGAKLQRAL"
BASE COUNT          521 a          312 c          406 g          416 t
ORIGIN      
        1 ctgaagggac acgttctgct gatggtacga ttcatcggtt taaggacggg gcttttattc
       61 ttgcccaaaa gtctgaaaag ccaattttac ctgtagtaat agatggttct ggcgaaataa
      121 tgcaaaaccc cctgaagcta aagttggcgc aaacccttac cattaaaatt ctacccgaga
      181 tttcatatga gagtttcagg gaattttccc ctaacgagct ggcaaggcac gtccagcagc
      241 taatggaaaa agagcacatg gctattgctc ccgaaaggta tcatggcagt atggttaaca
      301 gctagccata ttaactttgg tgaagttgta ggtaagtcaa attaaaaaaa atggttcaaa
      361 acatttgtcg taaagtaaaa cttttatatt tttgcaagcc caaaatatgt taatcatttc
      421 attctgaaaa taatgtatgc aattgttgat attgtaggcc agcagtttaa ggttgaaaag
      481 gatcagaaac ttttcgtcaa ccgcatggct ggggaaaccg gtaccgaggt aaaatttgat
      541 aaggtactgc tagttgacaa cgatggggct gtaaccgttg gaaagcctca ggtagaaaac
      601 gcatcggtta cagcgaaaat tcttcgccat gtaagaggcg ataaggttat cgttttcaag
      661 aaaaaacgtc gcacaggcta taagaagaaa aatggccatc gtcagaatta caccgagatt
      721 caaatatcag aaatcgtagc ttaacatttt aaaggtagcg aatcatggca cacaaaaaag
      781 gagttggtag ttcgagaaat ggcagggaat cgcatagtaa acgcctgggt gttaaacttt
      841 ttggggggca ggttgctaag gctggtaata ttattgtacg ccagcgtgga acaaagcaca
      901 atcccggtca aaacgttggg atgggacgcg atcataccct atatgccctt gtagatggcg
      961 aggtgagctt ccgtaaaaaa accgacaaca ggtcatacgt atcggttatc cccaaagaag
     1021 cttgaggtag gtaaaaaaat acttttaact ttgatctcct gctcaatgct gaatgtgttg
     1081 tgcaggattt tttgttattt taaaatatgc cgtcatgctt acactgaagc agattcgtga
     1141 gaataaggag gaagttatcc gtcgtttggc tgtaaagcac ttcgatggag ctgcaatcgt
     1201 cgggaaaatt atagagctgg atgatagacg gaaggcaatt cagctacaac tcgacaccaa
     1261 cctggccgag caaaatagcc ttgcacgtca gatcggaaaa ctttttgccg agggcaaatc
     1321 tgttgaagca aatgatgcta aggctagaac aaccaacctt aaggagcagt caaagctact
     1381 aacgcagcag ctggccgggg tggaagccga acagaggcag cttctacttc agatacctaa
     1441 tttgccgcac gattctgtac caaaaggggt tggtgccgac gataatgtta ttgttcgttc
     1501 gggaggtgaa atacccgaat taccgggaga tgtgcttcca cattgggatt tagccaaaaa
     1561 atacgacata attgactttg agctgggtgt aaaaatcacc ggaagcggat tcccggttta
     1621 caaggggaaa ggggcaaagc tacagcgggc gctta
//
LOCUS       DAOOQX010000493         1653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_95354_length_1653_cov_2.971840, whole genome shotgun sequence.
ACCESSION   DAOOQX010000493 DAOOQX010000000
VERSION     DAOOQX010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95354_length_1653_cov_2.971840"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..390
                     /locus_tag="PLX53_07775"
     CDS             205..390
                     /locus_tag="PLX53_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73015.1"
                     /translation="MEILLLLNNRSFSRGRISMYKMFDFGYGQIEENKEIMPEKCSTL
                     HFRNPNMDKNHLRGNLT"
     gene            499..954
                     /gene="rplM"
                     /locus_tag="PLX53_07780"
     CDS             499..954
                     /gene="rplM"
                     /locus_tag="PLX53_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOY73016.1"
                     /translation="MDTLSYKTVSANKATVTKEWVVIDATNQVLGRLSSQVAKILRGK
                     HKPNYTPHVDCGDNVIIINAEKVKLTGHKLTQKQYVRHTGYPGGQRFSTPKEMLQRKP
                     IWVVEHAVKGMLPKNRLASDLFRNLHVYEGPEHPHEAQKPRVLDLSTFK"
     gene            963..1349
                     /gene="rpsI"
                     /locus_tag="PLX53_07785"
     CDS             963..1349
                     /gene="rpsI"
                     /locus_tag="PLX53_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOY73017.1"
                     /translation="MQIYNGLGRRKSAVARVYLREGKGNIVINGRDMKDYFQSELLQY
                     VVMQPLNVLNVGGNFDIKVTLDGGGVKGQAEAVRLGISRALVEYNPDNKPTLKANGFM
                     TRDSRSVERKKPGRPKARKRFQFSKR"
     gene            1460..>1653
                     /locus_tag="PLX53_07790"
     CDS             1460..>1653
                     /locus_tag="PLX53_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOY73018.1"
                     /translation="MSRTSFQELLDAGVHFGHLKRKWNPKMAPYIFMEKNGIHIIDLH
                     KTVVKIDEAAAAVKQIAKSGR"
BASE COUNT          536 a          350 c          379 g          388 t
ORIGIN      
        1 cgttggcttt cgctcaactc gtcaaggtag ctgtgcatgg tgtggtattt tcaacctgca
       61 aaaatagtcc tgaaaaatgg ctatacaaaa aatgaccggc aaaacatcgg ttgaaagagc
      121 ttcgataatt ataagtctca taatatcagc accgaaggaa cagttttaga agaatagacg
      181 attgaaggaa aacacatcgg gacggtggaa attttacttc tactaaacaa ccgcagcttc
      241 tcccggggcc gaatttccat gtacaagatg ttcgattttg gttatggcca aattgaggag
      301 aacaaagaaa ttatgcccga aaaatgctca accctgcatt ttcgaaaccc aaacatggat
      361 aaaaaccatt taaggggaaa cttaacataa aaaggtgttg tggtttaaat taatagttat
      421 acctttgcaa cccacttgat gtttaaaaat gcaaggatca caaaacaaca ggtattaact
      481 taaaactagg agtttacagt ggatacacta agctacaaga cggtttcggc caacaaggca
      541 accgttacaa aagagtgggt tgtgattgat gccaccaacc aggttctggg taggttgtcg
      601 agccaggttg ccaagatact tagaggcaag cacaaaccca actacacgcc acacgttgac
      661 tgtggtgaca acgttattat cattaatgct gagaaggtta agctcaccgg ccataagctt
      721 acccagaaac agtacgtacg acacacaggc tatcccggag gacaacgatt ctcgaccccg
      781 aaggaaatgc ttcagcgcaa gcccatttgg gttgttgaac atgctgttaa gggtatgctc
      841 cccaaaaaca ggcttgcatc ggatctgttc aggaacctac atgtatatga gggtcccgag
      901 catccgcacg aggcacaaaa gccaagagta ctagatttga gcacttttaa atagtttaag
      961 gtatgcagat ttacaacgga ttaggaagaa gaaagtcggc tgtagccaga gtttacctgc
     1021 gcgagggcaa aggtaacatc gttattaacg gccgggatat gaaggactac tttcaatcgg
     1081 agctgctgca gtacgtcgtt atgcaaccac ttaacgtact taatgttggt ggaaatttcg
     1141 atatcaaggt tacccttgat ggtggcggtg ttaagggcca agccgaagca gtaaggctgg
     1201 gtatctccag agcgctggtt gagtacaacc ccgataacaa gcccaccctt aaggcaaatg
     1261 ggtttatgac acgcgattca cgcagcgttg agcgcaagaa acccggtagg ccaaaggcta
     1321 gaaagagatt tcaattcagc aaacgttaaa attataaatt ttcccatagt agtttggcaa
     1381 gcagtttagt gataaattgc ccagactccc ccacgggact actgtgcgat tgccaattaa
     1441 tctaaactaa aaaacaacca tgtcaagaac atcatttcag gagttgctcg atgcaggagt
     1501 gcactttggc catctaaaac gcaagtggaa tcccaagatg gcgccataca tcttcatgga
     1561 gaagaatggc attcacatca tcgatctcca caaaaccgta gtaaaaatag atgaggctgc
     1621 tgctgcagta aagcaaattg ccaaatcggg acg
//
LOCUS       DAOOQX010000494         1653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_95374_length_1653_cov_2.493742, whole genome shotgun sequence.
ACCESSION   DAOOQX010000494 DAOOQX010000000
VERSION     DAOOQX010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95374_length_1653_cov_2.493742"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLX53_07795"
     CDS             complement(<1..235)
                     /locus_tag="PLX53_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73019.1"
                     /translation="MNETLSKEEFSNLILKVQRDEINGFKIYSKLAKIEKDASNREIL
                     SRIAAEEYSHYNFFKSISGREVGHNRLTTWFYFT"
     gene            complement(384..1142)
                     /locus_tag="PLX53_07800"
     CDS             complement(384..1142)
                     /locus_tag="PLX53_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY73020.1"
                     /translation="MKIALITGATSGIGRATALRLAQDNFNLIVTGRRLTQLEQLKKE
                     VEVKFKKEVHILNFDIRFREQVDSAIDSLPEHWRKIDVLVNNAGLALGLNHLQDGMVE
                     DWEQMIDTNIKGVLYITRKVAPLMIERGGGHIVNIGSTAGTQVYENGNVYCATKHAMH
                     ALSQSMRQDMLKHNIRVTAVRPGLLETEFSLVRFKGDKKKAKDTYAGLPPLSAEDIAE
                     VIYFALTRPPHVCINDIEVTPTAQANSYYTNRKV"
BASE COUNT          400 a          366 c          378 g          509 t
ORIGIN      
        1 gggtgaagta gaaccaggtg gttagcctgt tgtgtcctac ctccctgccc gaaatgctct
       61 tgaaaaagtt gtagtggctg tactcctcag cggcaattcg ggataggatt tcccgattcg
      121 aagcatcctt ttctattttg gccagcttgc tgtaaatctt aaatccgttt atttcatccc
      181 gttgtacttt caaaatgagg ttgctaaact cctccttgct caacgtttca ttcatagtgc
      241 attaggtttg agaaatattt tcgtttaaaa gggatttcaa ccctcaacaa tagtatgaat
      301 caattcttga tgtaaaagta gggtaaaatt agctattttc tgaagtgaca gcacgaccgg
      361 caaaaaaaag tctgagccga agctcagact tttctatttg tgtagtatga atttgcctgg
      421 gcggtagggg taacctcaat atcgttgatg caaacatgag gtggacgtgt aagcgcaaag
      481 tagataacct ctgcgatatc ctcggccgaa agagggggga gaccggcata ggtgtccttt
      541 gcctttttct tatcgccctt aaaccgcacg agcgagaact cggtttccag aaggcccggc
      601 ctaaccgcgg ttaccctgat gttatgcttt agcatatcct gcctcatgga ctgtgagagg
      661 gcgtgcatgg cgtgcttggt agcgcagtaa acattgccat tctcgtaaac ctgggttccg
      721 gccgtggagc caatgttgac aatgtgtcca cctccccgtt caatcatcaa aggcgctact
      781 ttgcgggtga tgtacagaac acccttgatg ttggtatcga tcatctgctc ccagtcctcc
      841 accatgccat cctgcaggtg attcaacccc agcgccagac cggcattgtt aaccaacacg
      901 tcaatctttc gccagtgctc cggcaggctg tcaattgcgc tatcaacctg ctccctgaag
      961 cgaatgtcaa aattcaggat gtgcacctcc tttttaaact tcacctcaac ttcctttttc
     1021 agctgttcca gctgggtaag ccggcgcccg gttactatca ggttgaagtt gtcctgggct
     1081 agtcgtaggg ctgttgcacg gccaattcct gaggttgctc ctgtgattaa tgctattttc
     1141 atccgtacaa aattttagta ttattaatta gcaatcaatt agctgcgacc tggtattcta
     1201 cactcgttaa cgtttggata aaacctcaat ggcagcaggt aaacaaaccc ctaattgttt
     1261 agcttagcaa agctcgcaat tgacgctcgc ttaatcctct tttttaacca ggctatccta
     1321 taggggtatt ccattgttat gttgtaatgc tttgcatatc aatatgtaga cacaatttta
     1381 tgtaaaattt aagcggtgtc aaacgtactg taaaattttg tattgcaggc tgcttttcgt
     1441 cattttttac ctattcatgt tcagtggata cagcgtttaa agggtaaatg gtttggttta
     1501 tatggaggaa gtaatgtctg gttggctgag tctgggttaa aagtatatac aagtggatgt
     1561 tgatgtacag cttgattaga attctttgct aaaagccttg tttgtagctg ctgtggttgc
     1621 agctgcctcg tttatcccgg tttggcgcag ggc
//
LOCUS       DAOOQX010000495         1652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_95478_length_1652_cov_2.755792, whole genome shotgun sequence.
ACCESSION   DAOOQX010000495 DAOOQX010000000
VERSION     DAOOQX010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95478_length_1652_cov_2.755792"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1652)
                     /gene="dnaE"
                     /locus_tag="PLX53_07805"
     CDS             complement(<1..>1652)
                     /gene="dnaE"
                     /locus_tag="PLX53_07805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOY73021.1"
                     /translation="NSRFDRTDKEDRHNFHLILLAKNLEGYHNLVKLVSYGYTEGFYY
                     KPRIDKELLRQYSKGLIASSACLGGEIPQAILNNNIAEAERLIMEYRDIFGDDFYLEL
                     MLHKSGEPRIDSEVYENQLKVNKALLELSVKTGVKCITTNDVHFVMSDDAASHDLLIC
                     LNTDKRIDDTNRMRYTFQEYLKSEEEMLSIFSEHPEAVHNTMEILDKVEFYDLSHKPI
                     MPNFPLPDGFTDDNDYLRHLTYEGAKLRWGDTLSDEIGSRIEFELQVIERMGYPSYFL
                     IVWDFIRAAREMGVSVGPGRGSAAGSVVAYCLKITDIDPIKYNLLFERFLNPDRISMP
                     DIDIDFDEDGREDVLQYVVQKYGEKRVAHIVTFGTMAAKSSIKDVARVLNLDLSTSER
                     LSKLVPERIGITLPAAIKEVPELEKELKSDNELVRATLKHAQVLEGTIRQTGVHACGI
                     IIGRDDLENYIPLCINKEAKLFVTQYEGNHMEDIGLLKMDFLGLKTLSIVKDALQLIM
                     ESTGKSLDIDNIPLDDSTTLELFSRGDTTAIFQFESAGMKKY"
BASE COUNT          372 a          391 c          439 g          450 t
ORIGIN      
        1 gtattttttc attccggccg actcaaactg gaaaatggcg gtggtatcgc cccgggagaa
       61 gagttcgagt gttgtcgagt catccaaggg gatattgtct atatcgagcg attttccggt
      121 tgattccatg ataagctgca gggcatcctt aacaatggaa agggttttca gtccaagaaa
      181 gtccattttc aggaggccaa tgtcctccat atggttgccc tcatactggg taacgaagag
      241 tttggcctcc ttgttgatac aaagggggat gtagttttcc aaatcgtcgc gtccaatgat
      301 gatgccgcag gcatggacac ctgtttgtct gatggttcct tcaagaacct gtgcgtgctt
      361 tagtgttgcc ctcaccagct cgttgtccga tttcagctcc ttttccagct cgggaacctc
      421 tttaatggca gccggcaggg taatgccaat gcgttcggga acaagtttcg acaaacgctc
      481 ggaggttgag aggtcaaggt ttaacactcg ggcaacatcc tttatggacg atttggctgc
      541 catggtgcca aaggttacaa tgtgggccac acgtttttcg ccgtactttt gaaccacgta
      601 ctggagcaca tcctcccggc catcctcgtc aaaatcaata tcaatatcgg gcatggagat
      661 gcggtcgggg ttgagaaacc gctcgaacag caggttgtat tttatggggt ctatgtcggt
      721 aatttttagg cagtaggcga ccacggagcc agcggccgaa cctcgtccgg gaccaaccga
      781 tacccccatt tcgcgggcag cccttataaa gtcccaaaca atcaggaagt agctggggta
      841 gcccatacgt tcaattacct gaagttcgaa ctctattcta ctgccgatct cgtcgctaag
      901 ggtatcgccc cagcgcaatt ttgctccttc gtaggtaagg tggcgcaggt aatcattgtc
      961 atcggtgaaa ccatcgggaa gcgggaagtt aggcataatg ggcttgtggc taagatcgta
     1021 aaattcaacc ttatccagta tttccatggt gttgtgaact gcttcgggat gctcggaaaa
     1081 gatggaaagc atttcctcct cgcttttcag gtactcctga aaggtgtagc gcatacggtt
     1141 ggtgtcgtca atccgcttgt cggtgttaag gcatattagc aggtcgtgcg aggcggcatc
     1201 atcggacatg acgaagtgga cgtcgttggt ggtaatgcac tttactcctg tttttaccga
     1261 cagctctaac aatgccttgt ttaccttcag ctggttttcg taaacctcgg agtcaatgcg
     1321 gggttcaccg gatttgtgga gcatgagctc caggtaaaag tcgtcgccaa aaatatccct
     1381 gtactccatt attagcctct cggcttcggc gatgttgttg ttaagtatgg cttgaggtat
     1441 ttcgcctccc aaacaggcgc tggaggcaat tagccccttg ctgtactgtc gcaggagctc
     1501 cttatcaatg cggggcttgt agtagaaacc ctcggtgtag ccatagctaa ccagcttcac
     1561 caggttgtgg taaccttcca ggttcttggc cagaagaata aggtgaaagt tatggcggtc
     1621 ctccttgtcc gtgcggtcga aacggctgtt tt
//
LOCUS       DAOOQX010000496         1651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_95553_length_1651_cov_3.396617, whole genome shotgun sequence.
ACCESSION   DAOOQX010000496 DAOOQX010000000
VERSION     DAOOQX010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95553_length_1651_cov_3.396617"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..>1651
                     /locus_tag="PLX53_07810"
     CDS             400..>1651
                     /locus_tag="PLX53_07810"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOY73022.1"
                     /translation="MNSLLNRINSSRLRSIDYFRRHPQEVQEQTLFNLLSRAKNTEYG
                     QLNGFSRFKSIADFQQGLPVMEYEDFKPYIDRMRSGEENLLWPGKISWFAKSSGTTQN
                     KSKFIPVSREGLNGCHFRGSRDVIAIYFDLYPKNGLFRGKGLTLGGSHQVDTLSGSSR
                     YGDLSAILLQNQPWWTDFIRTPSQRVALIPDWEQKLEKITGETLAQNVTSITGVPSWN
                     LVMIKHILNHTGKSNLLEVWPNLELFIHGGVSFTPYRDQYFKLIPSPQMHYMEAYNAS
                     EGFFAIQDNPETDDMLLMLDYGVFYEFIPAEQLGQPNPPAYPIGEVEVGKNYAMLITT
                     SSGLWRYLIGDTVTFTSKSPYKIKITGRTRHFINAFGEEIIIDNAEKAIKKACDATGA
                     QVAEYTAAPVYMETDKKGSHEWLIE"
BASE COUNT          455 a          399 c          404 g          393 t
ORIGIN      
        1 gatgccattt accaggattc atacagggcc aaggagctgg gtgttaatgc tgttccttcc
       61 ctgctgatta acggcaggaa gctgctaagc ggagcacata agtacgaaac actaaaggag
      121 ctgactcaga gagaacttat aggaaaatag cttggtttgt gctctggtct gaccgggttg
      181 aagaactaga acttgttcaa caccctggtc agactttttt ttgcatcttc tatacggtat
      241 aaacccatat taattgagaa gaacgccggt ccggggagac aaccgtacca taccggttgt
      301 tgaaggcatt tgtttacttc tcctatgcaa agggatggaa atttgtagat ttagtttaaa
      361 tttgctagga aatcgaacaa ctttaagggt atgcctttgg tgaattcact cctcaaccgg
      421 attaactcca gccgtttacg cagcattgac tacttccgaa ggcacccgca ggaggtgcag
      481 gagcaaaccc ttttcaacct gctttccagg gcaaaaaata cggagtatgg acagctgaac
      541 ggatttagca ggttcaaatc catagccgat tttcaacagg gactacctgt aatggagtat
      601 gaagacttca aaccctacat agaccggatg agatccggtg aggagaacct gctttggccc
      661 ggtaaaatta gctggtttgc caaatcttcg ggaacaacac agaacaagag taaatttata
      721 ccggtaagcc gggaggggtt gaacggatgc cacttcaggg gaagccgcga tgtgatagct
      781 atttactttg acctgtaccc taaaaatggc ctttttagag gcaagggact taccctgggc
      841 ggaagccatc aggtggatac cctttcgggc agctcccgct acggcgattt atcggctata
      901 ctcctgcaaa accaaccctg gtggaccgat tttatccgca ctcccagcca acgggttgcc
      961 cttatccccg actgggagca gaagctggaa aagattaccg gcgaaacctt ggcccagaac
     1021 gtaaccagca ttaccggtgt gccatcatgg aacctggtga tgattaagca tattctgaac
     1081 cacaccggaa agtcgaacct gctggaggtt tggccaaacc tggagctatt cattcacggt
     1141 ggcgttagct ttacgcctta ccgcgaccaa tactttaagc taattccctc accccagatg
     1201 cactacatgg aggcctacaa cgccagtgag ggtttttttg ccattcagga caacccggaa
     1261 accgatgata tgctgctgat gctcgactat ggcgttttct acgagtttat tcctgccgaa
     1321 cagctggggc agcccaatcc cccggcctac cccattggtg aagttgaggt gggtaaaaat
     1381 tacgccatgc tgattaccac cagttcaggg ctgtggcgat acctgattgg cgatacggta
     1441 accttcacct caaaatcacc ctataaaata aagataaccg ggcgaacccg acatttcatc
     1501 aacgcttttg gcgaggagat cattattgat aacgccgaaa aagcaattaa gaaagcctgc
     1561 gatgcaactg gggcacaggt tgccgagtac accgcagccc cggtttacat ggaaaccgat
     1621 aaaaagggta gccacgaatg gcttatcgag t
//
LOCUS       DAOOQX010000497         1646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_96039_length_1646_cov_3.161534, whole genome shotgun sequence.
ACCESSION   DAOOQX010000497 DAOOQX010000000
VERSION     DAOOQX010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96039_length_1646_cov_3.161534"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLX53_07815"
     CDS             <1..472
                     /locus_tag="PLX53_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOY73023.1"
                     /translation="AFLSIAVGYIPFGNFISSDSMPLESHINWGVASASIIVSFTGII
                     IAWVLYKKPSNLPERIVSSIKVLYTWAYHKFYIDEVYLFVTKKIIFNLISRPIAWFDR
                     HVVDGAMNGIAWTTSWVSESIKGLQSGQLQKYALAFVSSTIILSALLIYILLNV"
     gene            485..>1646
                     /locus_tag="PLX53_07820"
     CDS             485..>1646
                     /locus_tag="PLX53_07820"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009235979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOY73024.1"
                     /translation="MNLSLFVIIPLLTVLLILFSKSIRQTRLIAAIGMSVQLVLSAIL
                     VVLYVSLRKHGNADQFVFVKDIEWFKSLNFNFTMGVDGISVALIGLTSIVIFAGVFAS
                     WKMEDLHREFFISLLVLAGGVFGFFITIDMFSMFVFYELAVIPMYILIGIWGTGKKEY
                     SAMKLTLMLMGGSALIIVGILGLYFHSGVSGGPLTMNLMNLSKVHFPVEVQMFLFPFL
                     FIGFGVLGALFPLHTWSPDGHASAPTAVSMLHAGVLMKLGSYGVFRIAIYLLPEAARQ
                     MGPFFMVLATIGIVYGAFGAIMQKDLKYINAYSSVSHCGLILFGLLMMNKTAMDGAIL
                     QMISHGIMTALFFALIGMIYGRTHTRLINEMGGLLKVMPFLGAAYVIAGLASL"
BASE COUNT          368 a          368 c          383 g          527 t
ORIGIN      
        1 agcctttctc tccattgctg ttggctacat accctttggt aattttattt cttctgacag
       61 tatgcccctg gaaagtcata taaattgggg agttgcatct gcttccatta ttgtaagctt
      121 taccggaatt attattgcat gggtgcttta caagaaaccc tccaacctgc ccgaaagaat
      181 tgtcagcagc atcaaggtgc tctacacctg ggcctaccat aagttttaca ttgatgaggt
      241 atatcttttt gtaaccaaaa aaatcatttt taacctcatt tcaaggccaa ttgcctggtt
      301 cgacaggcat gtggttgacg gggcaatgaa tggcatagca tggactacca gctgggtttc
      361 cgaaagtatt aagggtttgc aatcgggaca acttcaaaaa tacgctcttg cattcgtttc
      421 aagcaccatt attttaagcg cattgcttat ctacattttg ttgaacgtct aacatggtga
      481 gaatatgaac ctttcgcttt ttgtaatcat cccgctacta accgttttgc ttattctttt
      541 ttctaagagt ataaggcaaa cacggttgat agcggctatc ggaatgtctg ttcagctggt
      601 gcttagcgca atactggttg tgctgtatgt tagcctgcga aaacatggga atgccgatca
      661 atttgtgttt gtaaaggata tcgagtggtt taaaagctta aatttcaact tcaccatggg
      721 ggttgacggc atttcggttg ccctaatcgg ccttacctca atagtcattt ttgccggtgt
      781 gtttgcctcg tggaagatgg aagacctgca tcgcgagttt ttcatctcgc tactcgtgct
      841 tgcgggtggg gtgtttggct tcttcatcac catcgacatg ttctccatgt ttgtttttta
      901 cgagttggcg gtgataccca tgtacatttt aattggtatt tggggaaccg gcaagaagga
      961 gtactcggcc atgaagctca ccctgatgct catgggaggt tctgcactta tcattgtggg
     1021 tattttgggt ctatacttcc attcgggtgt aagcggcggc ccgctaacca tgaacctgat
     1081 gaacctgtcc aaagtgcatt ttcccgttga ggttcagatg ttcctgtttc ctttcctctt
     1141 tattggtttt ggcgttttgg gtgcattatt tcccctgcat acatggtcgc ccgatggaca
     1201 tgcctcggca ccaaccgctg tttctatgct tcatgccgga gttcttatga agctcggaag
     1261 ttacggcgtt tttcgtatcg caatttatct cctacccgaa gcagccaggc aaatgggacc
     1321 attttttatg gtgctggcaa caatcggaat cgtgtacgga gcctttgggg ccatcatgca
     1381 aaaggactta aagtacatta acgcttactc gtccgttagc cactgcggac tcatcctttt
     1441 tggattgctg atgatgaaca agacggccat ggatggcgct atcctccaga tgatctccca
     1501 tggaattatg accgccctct tctttgccct tatcgggatg atatacggcc gcacccacac
     1561 gaggttgatc aacgagatgg gtggactttt aaaggtgatg ccatttcttg gcgccgctta
     1621 tgtaattgca ggtcttgcct cgcttg
//
LOCUS       DAOOQX010000498         1645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_96164_length_1645_cov_2.559119, whole genome shotgun sequence.
ACCESSION   DAOOQX010000498 DAOOQX010000000
VERSION     DAOOQX010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96164_length_1645_cov_2.559119"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1617
                     /locus_tag="PLX53_07825"
     CDS             193..1617
                     /locus_tag="PLX53_07825"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOY73025.1"
                     /translation="MLLQYLSLMRQEVSLIFIAVLVLICELTVNQRYKKVVIPITLVL
                     FGVHTILGFVPFDGGKLFGGMFATSHAQALMKSVLNVGVFIILLQSVAWLKKEVNFQK
                     SNEFLILLFSTLTGMYFMISSGDFLMFYIGLEIASIPMAMLAAFDTFRSKSSEAGIKF
                     LLNSALSSGILLFGISLFYAISGTIYFDDLPLYFSPVSPLFALGFIFFISGLAFKMSL
                     VPFHLWTADVYEGSPTIVTSYFSVISKGAAAFITSILLFKVFFNQLYLWKDIIYLLSV
                     LTMTVGNLFALRQTNIKRFLAFSSISQAGFILLGLVNVDSLGVTAIIYFILIYILSNL
                     SAFGVVMAIANVTGKEEIKDYNGLYKTNPLLSLTMMLAMFSLAGIPPTAGFFSKFFLF
                     TDAAKGGYYWLVFIAVLNTIISLYYYLLVVKAMFISPNEQPIERFRSDIYTRTALLIT
                     VVGIFAIGIYSPIFEYIRNIIDKF"
BASE COUNT          396 a          377 c          319 g          553 t
ORIGIN      
        1 cctgtcaaaa acgaacacca cctgctgctt accgatgcaa cctggtggga acggctttcg
       61 gctggtacct tgattctttt tatcactata ataggttttt atccctattt tcttaccaac
      121 gacctcattg gtaatagctt ggaacccatt ctacaaaaaa ttttgatgtt taactaactg
      181 ataacctaaa ttatgctatt acaatatctt agcctaatgc gtcaggaggt atcgctgatt
      241 tttatagctg tgttagtatt gatatgtgaa ttaactgtaa accaacgata taaaaaagtt
      301 gttatcccca tcaccctcgt attatttggc gtgcacacca ttttgggatt cgttcctttc
      361 gatgggggta agctgttcgg cggaatgttt gccaccagcc atgcccaagc cctgatgaag
      421 agcgtactta acgtaggcgt ttttattatt ctactccaat cagtagcatg gctgaagaag
      481 gaagtaaatt ttcagaaaag caacgagttc ctaatcctgt tgttttccac ccttaccggg
      541 atgtacttta tgatttcatc aggcgatttc ctgatgttct acattggact tgaaattgca
      601 tcaattccta tggccatgtt ggctgctttt gatactttca ggagcaaatc atcggaggcg
      661 ggaataaagt ttctcctaaa ctcggctttg tcgtcgggta ttttgctgtt cgggatttcc
      721 ttgttttatg ccatcagtgg aaccatttat tttgatgacc tgccgttgta cttttcgcct
      781 gtttcacccc tctttgccct gggattcata ttcttcattt ctggcttggc ttttaagatg
      841 tcgctagtcc cattccatct ttggacagcc gatgtttacg agggctctcc caccattgta
      901 acctcatatt tctccgttat ttctaagggt gcggctgcat ttatcacctc catactgctc
      961 tttaaggttt ttttcaacca gctttacctt tggaaggata tcatttacct gctctcggtg
     1021 ctcacaatga ccgttggtaa cctgtttgcc ctccggcaaa ccaacatcaa aagatttctg
     1081 gcattctcat caatctccca ggccggcttt attcttcttg gtctggtaaa cgttgattca
     1141 cttggagtta cagctattat ctactttatt ttgatctaca tactttccaa cctatccgca
     1201 tttggcgtgg taatggctat tgcaaatgtt acagggaagg aggaaataaa ggactacaac
     1261 ggcttataca aaaccaaccc gttgctatcg ctaaccatga tgcttgccat gttctcgctt
     1321 gccggcatcc cgccaacggc tggctttttc agcaaatttt tcctttttac tgatgcggca
     1381 aagggtggct actactggct tgtttttatt gccgtgctaa ataccatcat ttcgctctac
     1441 tactacctcc tggtggttaa ggccatgttt attagcccta acgaacagcc aattgaacgc
     1501 ttcaggagcg atatttatac ccggactgcg ctgctaatca cggttgtggg aatttttgct
     1561 atcggcatct atagcccgat attcgaatac atacgaaaca ttattgataa attttaaata
     1621 cggctatggg taaatttagc tcaaa
//
LOCUS       DAOOQX010000499         1643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_96369_length_1643_cov_2.852015, whole genome shotgun sequence.
ACCESSION   DAOOQX010000499 DAOOQX010000000
VERSION     DAOOQX010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96369_length_1643_cov_2.852015"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /gene="ispE"
                     /locus_tag="PLX53_07830"
     CDS             <1..799
                     /gene="ispE"
                     /locus_tag="PLX53_07830"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOY73026.1"
                     /translation="INIGLNIVDRRSDGFHNISTFFYPVKGLCDMLEVIIAEDQDLPL
                     TFSQTGVETNCPANENLCVRAVGLMSKRLALPPIAMHLHKVIPTGAGLGGGSADGSFT
                     LVAINELLGNPLGKKELMELSLQLGSDCPFFIENQPCLAEGRGEKIRPLKIDLSGYFL
                     LVVKTDIQVETGKAYSETKPAPWGVSLVNLIKDDINTWRLTIKNDFEEIIFTRYPKIG
                     KIKKRLYGMGAIYAGLSGSGSAVYGIFDKEPKGWVKREDYFIRCVRL"
     gene            complement(851..1210)
                     /locus_tag="PLX53_07835"
     CDS             complement(851..1210)
                     /locus_tag="PLX53_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOY73027.1"
                     /translation="MTKIDINGNTHDTPKLDDSQLNTLAVEDGEYKTLFTPKFSGYKK
                     WEQHNEKLIRAFIPGTIVEVFVKPGQPVKQGDMLLILDAMKMNNQVKSPINATVKEVN
                     VKVGDKVSKGVDLVVLE"
     gene            complement(1222..>1643)
                     /locus_tag="PLX53_07840"
     CDS             complement(1222..>1643)
                     /locus_tag="PLX53_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOY73028.1"
                     /translation="IRHGAKLLYAYSEATVPKLTVILRKAYGGGYIAMSSRHLRADFV
                     FAWPGAEIAVMGPEGAANIIFRKEISEAANPEEMRRQKVEEYKEKFANPYVAAAKGYI
                     DTVIEPAETRKLLLHALEVASTKVSDRPFKKHGIPPF"
BASE COUNT          442 a          392 c          377 g          432 t
ORIGIN      
        1 aatcaacatc ggactcaaca ttgttgaccg acgtagcgat ggtttccata atatttctac
       61 ctttttctat ccggtaaagg gtttatgcga tatgctggag gtcataattg ccgaagatca
      121 ggatttgccc ttaaccttca gccaaacagg agttgaaaca aactgccctg ccaacgagaa
      181 cctttgcgta agggcggttg gactaatgag taaacgactg gctttacccc caattgccat
      241 gcacctgcac aaggttatcc ccaccggagc tggtctgggg ggtggttcgg ccgatggctc
      301 ctttacgctt gttgccatta acgagctgct tggcaatcct ttagggaaaa aggaattgat
      361 ggagctatcc ctgcagcttg gcagcgattg ccctttcttt atcgaaaatc aaccctgcct
      421 tgccgaaggg cgtggcgaaa aaattagacc cttgaaaatt gacctctccg gatacttcct
      481 gctggttgtg aagaccgaca ttcaagttga aaccggaaaa gcgtactccg aaactaaacc
      541 tgccccatgg ggcgtgagcc tcgtaaacct tataaaggac gatataaaca cctggcggtt
      601 gaccattaaa aacgattttg aggaaattat tttcacccgc taccccaaaa tagggaaaat
      661 aaaaaaaagg ctgtacggta tgggtgcaat ttatgcgggg ttatcgggaa gtggatcggc
      721 agtgtacggc atttttgaca aagagccaaa aggttgggta aaaagagaag actacttcat
      781 ccgttgtgta aggctataaa aaaaagggcg ctgtggcgcc cttttcatat agcatgcttc
      841 acaagtgtag ctactcaaga acaactagat caacaccctt gcttacctta tcgccaacct
      901 taacattcac ctctttaacg gtggcgttga tgggcgattt aacctggttg ttcattttca
      961 tggcatccag aataagcagc atatcaccct gcttaacagg ctgcccgggt tttacgaaaa
     1021 cctcaacaat agtacccggt ataaaagccc ggataagctt ctcattatgc tgctcccact
     1081 tcttgtagcc cgaaaacttc ggggtgaata aggttttgta ctcaccatcc tcaaccgcca
     1141 aagtattgag ctgtgaatcg tccaatttag gcgtatcgtg agtattacca ttaatatcaa
     1201 ttttcgtcat attccaactt tttaaaatgg aggaattccg tgttttttaa atggtctatc
     1261 ggaaaccttg gtgctggcca cctcaagagc atggagcaaa agcttgcggg tttcagcggg
     1321 ttcaatcacc gtatcaatgt aacccttagc agcagccacg taagggttgg caaatttttc
     1381 cttgtactcc tcaaccttct ggcgtcgcat ttcttcgggg ttggcggctt ccgaaatctc
     1441 cttgcggaaa atgatgttgg ctgcgccttc ggggcccata acggcaattt cagcacccgg
     1501 ccatgcaaat acaaaatctg cacgcaggtg gcgtgaactc atggcaatgt atcctccgcc
     1561 gtacgcttta cgtaaaataa ccgtcagctt gggaactgta gcctcgctgt aggcgtaaag
     1621 caacttggcg ccatgcctaa taa
//
LOCUS       DAOOQX010000500         1643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_96385_length_1643_cov_2.587531, whole genome shotgun sequence.
ACCESSION   DAOOQX010000500 DAOOQX010000000
VERSION     DAOOQX010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96385_length_1643_cov_2.587531"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PLX53_07845"
     CDS             <1..872
                     /locus_tag="PLX53_07845"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOY73029.1"
                     /translation="LPELEDLALPVEGTAHNIALVKFRDYYPGQAYKIMNAIWGAGQM
                     MFNKLLVALPKDIDLTNADQILHSLVNFVDPERDLMFGKGPLDILDHATPTQAFGSKL
                     FIDATNQTVVMPHLQISIKDVKELLERETSRVKNFNLNLLNRGISILIISVDKQAVTN
                     VKSYMQQLFRCQELAGLRAMVAVDMGDAVSDLSMLTWFASSNIDPIRDVFILNLNDGY
                     PARVGVDGTRKAYPQDNFKREWPNVVAMSREVVDHVNKQWEKYGIGGMVESPSKQLLP
                     LVAGEGPVVKLNS"
     gene            1131..>1643
                     /locus_tag="PLX53_07850"
     CDS             1131..>1643
                     /locus_tag="PLX53_07850"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOY73030.1"
                     /translation="MLLFFIKGGGELFAQKTEVEVFDANNGLPQTYVCCLIQDSKGFL
                     WLGTHSGLCLYDGYQFTTFSNNPLDSLSLCNNFVKSIAEDKNGDIWVATLSGLSRYER
                     ATGKFISYFSEPTNPASLAGDKIYYVYVDREKNLWVHTPGTLNLYSYKSNDFTRFNYF
                     NDLFTQPVETD"
BASE COUNT          455 a          353 c          363 g          472 t
ORIGIN      
        1 tgctaccgga gctggaggat ttggctcttc cggtggaagg cactgctcat aacattgctt
       61 tagttaagtt cagggattac taccctggcc aggcatacaa aataatgaat gcaatctggg
      121 gcgcagggca gatgatgttt aataagctac tggttgcttt accaaaagac atagacctta
      181 caaatgccga ccaaatactt cactcccttg ttaactttgt tgaccccgaa agggatttaa
      241 tgtttggtaa ggggcctctc gatatccttg accatgctac tcctacacag gcatttggaa
      301 gcaaactctt tattgatgcc accaaccaaa cggttgttat gccccacctt caaattagca
      361 tcaaagatgt aaaggagcta cttgaaaggg aaacttccag ggtgaaaaat ttcaacctga
      421 atcttctcaa caggggaatt tccattctta tcatttctgt tgataagcaa gccgtaacaa
      481 atgtaaaatc ctatatgcag caacttttcc gttgccagga gttggctgga ttaagggcta
      541 tggttgccgt tgacatgggt gatgctgttt ccgatttgtc tatgctaaca tggtttgctt
      601 caagtaacat cgacccgata agagatgtct tcatactcaa cctgaacgat ggttacccag
      661 cccgagttgg agttgatggc acccgcaagg cataccctca ggataatttt aagcgcgaat
      721 ggccaaatgt tgtggccatg agccgggagg tggttgacca cgtaaacaaa caatgggaaa
      781 aatatggaat tggcggaatg gttgaatccc cctcgaaaca gcttttaccc cttgtggctg
      841 gtgaagggcc ggtggtaaag ctcaacagct gaacttttac attgatatgg cttcaccata
      901 atcacttttt tgggactcat agctgcattc aacttactgt ttctgagtta gcgctgagca
      961 tccgcttaaa atttcctcag gagtaccgtt taatgctgca ggatataggc tactacttgg
     1021 gccaggtggt tatcatcctg ataaattcaa gcctagtttt gttatgctga aaaataagta
     1081 tattagcggg taccatgaga tgtaaagctg tttttaaatg cttgttgccg gtgctgcttt
     1141 tttttataaa aggcggaggt gaactttttg ctcaaaaaac agaggttgaa gtgttcgatg
     1201 ctaacaatgg cctgccccaa acctatgttt gctgtttaat tcaggattca aagggatttt
     1261 tatggcttgg aacccatagc ggcctttgcc tatatgatgg ctaccagttc actactttta
     1321 gcaacaaccc tttagattca ttgagccttt gcaacaactt cgttaaatca atagctgagg
     1381 acaaaaatgg cgatatttgg gttgccacac tatcggggtt aagccgctat gagcgggcaa
     1441 ccggaaaatt tattagctac ttttccgaac caaccaatcc tgcatccctt gctggggaca
     1501 aaatttacta cgtttacgta gacagggaga aaaacctttg ggttcacaca cccgggacat
     1561 tgaacctgta cagctataag tccaatgact tcactcgatt taactatttt aacgacctct
     1621 ttacccaacc ggttgaaacc gat
//
LOCUS       DAOOQX010000501         1639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_96748_length_1639_cov_3.136995, whole genome shotgun sequence.
ACCESSION   DAOOQX010000501 DAOOQX010000000
VERSION     DAOOQX010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96748_length_1639_cov_3.136995"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PLX53_07855"
     CDS             complement(<1..957)
                     /locus_tag="PLX53_07855"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOY73031.1"
                     /translation="MEWQVMAQAGDCFVDVVLPLALPRLYTYSIPDEMIGRVEQGMRV
                     VVSFGRKKLYAAVVFRVHSDAPNGYQTKDIIQLIDEQPVVLPGQLKFWSWVADYYMCY
                     LCEVMKAALPSGLKMESESFVSQANVEDINFSDSERIILHLLDGKKKRRIQDIATMAG
                     FKNPMGAIKSLLEKNAIFISEEIEEAYRPKTEKYLSISPKYCSNDAINELLNSLGRAP
                     AQQRLLLGMLSLIDNFSELSDVCVPKRKVLEKVDVSAAVVGSLVAKGVICEVEKAVSR
                     VNDADNQLGKPVELSSAQLEALNRVKESFATHHVTLLHGVTSS"
     gene            1271..1344
                     /locus_tag="PLX53_07860"
     tRNA            1271..1344
                     /locus_tag="PLX53_07860"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1305..1307,aa:Pro,seq:ggg)
     gene            complement(1418..>1639)
                     /locus_tag="PLX53_07865"
     CDS             complement(1418..>1639)
                     /locus_tag="PLX53_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73032.1"
                     /translation="KDRELLINNKRVHYLNCGDWVEHFTAAEYYNGSWHLYVQNETGD
                     ELLYDEPEIPAGRQLYQKVKLELAFSNLG"
BASE COUNT          450 a          420 c          345 g          424 t
ORIGIN      
        1 gctagaggtt acaccatgaa gcagggttac atggtgggtg gcaaacgatt ccttaactct
       61 attcaaagct tcaagctgag cactgctcaa ctccaccggc tttcccagct ggttgtcggc
      121 atcgttaacc cgcgaaacag ccttttcaac ctcacaaatt actcctttgg caacaagact
      181 accaacaaca gctgctgaga catcaacttt ttcgaggact ttgcgtttcg gcacacaaac
      241 atcggacagt tcagaaaaat tatcgatcaa cgaaagcatc ccaagcagca acctctgctg
      301 agccggagcc ctgccaaggc tattaagcaa ctcgttgata gcatcgttgc tacaatattt
      361 tggtgaaatt gacaagtact tctccgtttt tggtctgtac gcctcctcaa tctcctcgga
      421 gatgaagatt gcatttttct ccaacagcga cttaatcgcc cccataggat ttttaaaacc
      481 tgccatggtg gcaatatcct gtattctacg cttcttctta ccgtcaagca ggtgcaggat
      541 aatccgttcg ctatccgaaa agttaatatc ttcaacattc gcttggctca caaagctttc
      601 gctctccatc ttcaggccgg agggaagtgc agccttcatc acctcacaca aatagcacat
      661 gtagtagtcg gccacccaac tccaaaactt cagctggccg ggtagtacaa ccggctgctc
      721 gtcaataagc tggatgatgt ccttggtttg atacccattg ggggcatcac tatgaaccct
      781 gaacacaacc gcagcgtaaa gtttctttcg tccaaacgaa accacaaccc gcattccctg
      841 ttcaacccta cctatcattt catcgggaat ggagtaggtg tagagccttg gaagcgctag
      901 tggaagcaca acatcaacaa agcaatctcc tgcctgagcc attacctgcc actccaatta
      961 gccattacct gatgtaaaaa aaaacaatga gccctgcatc attaaaaact cgtgtgtaaa
     1021 ggaaaatatg caagtagaaa ccatctgcaa agtgcagccg gatttgttac acaagccctt
     1081 cgcctagccg ggagagctgg cttcctaaag taaaacatac gggcaaggta tgaaaatctt
     1141 tgcaggattg aataaaaatt tttgaaacaa actaaaaagg gtgtattttt gcaacgcttt
     1201 gctcagaaat tcgagagatg gctaattttt tcaaccattt tcggttatga agcagggcga
     1261 aattttgctt cggggtgtgg cgcagttggc tagcgcactt gcatggggtg caagtggtcg
     1321 tccgttcgag tcggatcacc ccgacaaaac agtaaaaggc aaccggtggt tgccttttta
     1381 atttcaatac ccggctattt aaaatttacc cggcgagcta ccccaggtta gaaaatgcca
     1441 actcgagttt aaccttctgg tacagctggc gacctgctgg tatttccggc tcatcatata
     1501 gcagctcatc cccggtttcg ttttgaacgt aaagatgcca ggaaccattg tagtactcgg
     1561 ctgcagtgaa gtgctccacc caatcgccac agttcaggta gtgaaccctc ttgttgttta
     1621 taagcagctc cctatcctt
//
LOCUS       DAOOQX010000502         1636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_97058_length_1636_cov_1.958254, whole genome shotgun sequence.
ACCESSION   DAOOQX010000502 DAOOQX010000000
VERSION     DAOOQX010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97058_length_1636_cov_1.958254"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="PLX53_07870"
     CDS             <1..1147
                     /locus_tag="PLX53_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73033.1"
                     /translation="GVRFTLAKKYRFSLQMAYDQLEFGRTPDSLQTKTALGTAANFTY
                     SASQLRVSVRYKAGEKYFYSGYNGRSELMAQADYNFKNSSRLIYNAIDNRNSPGDYRT
                     YGLTYQPYTNFHRQLLTYGVNFTPRFYLFTGPGFELNSTDIFPVIRRGEDYFSTIVYN
                     YNAGLRFKGSRPGSSLTTQMFAGWVNVAHQPTHFQDPGFSIPDSKNKFDYQFFSINLR
                     QPMWGAQAMYTNGPRSIYQQFSWYYSSRPTRLLRVMPYFDAFIYKDMLKLMVNVSYTN
                     DLVSKSSYSNITSQLYWYLPRDWQIRLLNVYTIQSRTTGTETVERFQNMYFEASVRKE
                     FGFQQPTVKYYDLKLVFFKDYNGNRIKNDNEPGIKNVLLSIQRTDTP"
     gene            1196..>1636
                     /locus_tag="PLX53_07875"
     CDS             1196..>1636
                     /locus_tag="PLX53_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73034.1"
                     /translation="MFFENIPAGIYDLSYNPVGTEAGTFSKADIESTIRFDKNTTLYI
                     PFVEKNKVFGKIILNRSKLSGLGKVDVSNVRITVTDSRGNTFSTLTDKNGEFMIFAPI
                     TDEYIVSINNIFYEDFNLRQNNFRVQFNGYKQFEVNFVFDEKVRR"
BASE COUNT          424 a          428 c          382 g          402 t
ORIGIN      
        1 aggcgtaagg tttacccttg caaaaaagta ccggttttcg ctgcaaatgg cctacgacca
       61 gctggaattt ggaaggaccc ctgatagcct acagaccaaa acagcgctgg ggacagctgc
      121 aaactttacc tactccgcct cgcagctgag ggtgtcggtg cgctacaagg ctggtgagaa
      181 atatttttac tcgggctaca atggtcggtc ggagcttatg gcccaggccg attacaactt
      241 taaaaatagc agccgactta tttacaatgc aatagataac cggaatagcc ccggagacta
      301 ccgcacctac gggctaacct accagcccta caccaacttt cacaggcagc tgcttactta
      361 tggggttaac tttactcccc gtttttacct gttcacaggg ccgggttttg agctcaactc
      421 taccgatatc ttcccggtta tcaggcgtgg cgaggactac ttctccacaa ttgtgtacaa
      481 ctacaatgcc ggcttgaggt tcaagggatc ccggccgggc agctcgttaa ccacccagat
      541 gtttgccgga tgggttaatg ttgcgcacca acctacccat tttcaggatc cgggcttttc
      601 aattcccgat agcaagaaca agttcgacta ccagtttttc tccatcaacc tgcgccagcc
      661 catgtggggt gcccaggcca tgtacaccaa cgggccacgc tccatctacc agcagtttag
      721 ctggtactac agctcccgac caacgcgttt gctccgggtg atgccctact ttgacgcctt
      781 tatctataag gacatgctga agctgatggt gaacgtctcc tacaccaacg acctggtttc
      841 aaaatcgagc tactccaaca tcacctcgca gctctactgg taccttccgc gcgactggca
      901 aatcaggctg ctgaatgttt acaccattca gtcgcgcacc acgggaaccg aaacggtgga
      961 gcggtttcaa aacatgtact tcgaggcaag cgtgcgcaag gagtttggtt tccagcagcc
     1021 caccgttaag tactacgacc tgaagctggt tttctttaag gactacaacg gcaaccggat
     1081 aaagaacgac aacgaaccgg ggataaagaa cgtgctgcta agcatacagc gtaccgatac
     1141 cccgtagttc cccgattttt catccggcga gctactatcc aaccagtacg gcgaggtgtt
     1201 tttcgagaat attcctgccg gaatttacga tctttcatac aatcctgtgg gtaccgaggc
     1261 cggaacattt tccaaggccg atattgagtc aaccattagg ttcgataaga atactaccct
     1321 atacataccc tttgttgaga agaataaggt ttttgggaaa atcattctca atcgcagcaa
     1381 gctatcaggt cttggaaagg ttgatgtttc gaatgtgagg attactgtaa ccgatagtcg
     1441 gggtaacacc ttctcaacgt tgactgacaa gaacggggag ttcatgattt ttgccccaat
     1501 caccgatgag tacatcgtta gcatcaacaa catattctat gaggatttta acctgcgcca
     1561 gaacaacttc agggtgcagt ttaacggcta caagcagttt gaggtgaact ttgtattcga
     1621 cgagaaggtt cgtaga
//
LOCUS       DAOOQX010000503         1635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_97100_length_1635_cov_3.329747, whole genome shotgun sequence.
ACCESSION   DAOOQX010000503 DAOOQX010000000
VERSION     DAOOQX010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97100_length_1635_cov_3.329747"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLX53_07880"
     CDS             complement(<1..265)
                     /locus_tag="PLX53_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOY73035.1"
                     /translation="MASENLNEELASERTKNFLEEIIEEDIKSGKHGGRVLTRFPPEP
                     NGYLHIGHAKSICLNFGLAQRYGGKTNLRFDDTNPAKEDVEFVD"
     gene            506..1519
                     /locus_tag="PLX53_07885"
     CDS             506..1519
                     /locus_tag="PLX53_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOY73036.1"
                     /translation="MILVTGGTGLVGAHLLYELTSRGESVRALKRERSNTEFVDWVFK
                     LYSHESSKLLQGIEWVDGDLLDYQSLLDATKGVQTVYHTGGIVSFNPLQSSSVIETNI
                     TGTGNLVDACMDNGVKEICHVSSIAALGQANESGMVDEDCRWIESKGQNAYAVSKLYG
                     ENQLWRGFAGGLRVIVVNPTVILGPGRWDSGSGQLFKRVYKGMPFYTNGVTGFVDVRD
                     VARAMVVLMEETGINGERFILNSENVSYRDIFTMMAASLGKRKPSIRVPAGVVDAVYP
                     LIQLFGQLSGRGNLVSKANLKTAFRVTLYSSQKIREKIGFEFIPIEETIRFVGKAFRN
                     AEP"
BASE COUNT          418 a          365 c          419 g          433 t
ORIGIN      
        1 cgtccacaaa ctccacatcc tcttttgccg gattggtgtc gtcaaaccgc aggttggttt
       61 tgccgccata cctttgggca agcccaaagt tcaggcatat tgatttggca tggccaatat
      121 gcaggtagcc gtttggctct ggtggaaacc gggttaagac ccttccgcca tgctttcccg
      181 acttaatatc ctcctcaatt atctcctcca gaaaattttt agtcctttcg gaagctaact
      241 cctcatttag gttctcgctt gccatcatat ttgggttgat tcacagaagc aaaagtagaa
      301 atttgccgct aaagtttatt agcacctaat aaattttaca ctccttccct taagtttaga
      361 cgtaaccgcc catataatgg cagcttcaaa tcacccgtaa atcttcaaca ttcctgattt
      421 ttaccaattt agctgaaatt ttgctatgtt tgaacctggg tgcagaatac ttgttcttaa
      481 tagcaaacca acagatggaa atagtatgat tttggtaacc gggggaaccg gactggtagg
      541 ggcgcatctt ctatacgagc taacttcacg cggcgaaagc gtgcgggcgt tgaagcgtga
      601 gcgctccaac actgaatttg ttgattgggt attcaagctt tacagccatg agtccagcaa
      661 gctgctacag ggaatcgagt gggttgatgg cgatttgctc gactaccaat cgctacttga
      721 tgcaacaaag ggtgttcaga cggtttacca tactggtgga attgtttcgt ttaaccccct
      781 gcagagctcg agcgtgattg aaaccaacat taccggaacg ggcaatttgg ttgacgcctg
      841 catggacaat ggggtaaaag agatttgcca tgtaagctcg atagccgcat tgggccaggc
      901 caacgaatcg ggaatggttg atgaggattg ccgatggata gagagtaagg ggcaaaacgc
      961 ctacgctgtc agcaagctgt acggggagaa tcaactttgg cgaggctttg ccggaggatt
     1021 gcgagttatt gttgttaacc caacggtaat tcttgggccg ggacgctggg atagtggaag
     1081 cggccaactc tttaagcggg tatataaggg catgcctttc tacaccaacg gcgttacggg
     1141 ctttgtagat gtgcgtgatg ttgcaagggc aatggtggtg ctcatggaag aaaccggcat
     1201 caacggtgaa aggtttatcc tcaactccga gaacgtcagc tacagggata tcttcacgat
     1261 gatggctgcc tcgctgggca aacgcaagcc cagcatcagg gtgccagccg gtgtagtgga
     1321 tgcggtttac ccacttatcc agctatttgg acagctctca ggaagaggga acctcgtttc
     1381 caaggccaac ttaaagacgg ctttcagggt aaccctgtac tcctcgcaga aaataaggga
     1441 aaaaattggg ttcgagttta tacccattga agaaaccatc aggtttgttg gaaaggcatt
     1501 taggaacgcg gagccatagc gcaacaatta ttccacactg gctttagggc aattgggttg
     1561 cagcatactt tgtttccaca tcactgcttg gttggcttgt caagggtgaa gctaaatgtt
     1621 gtccccttgt taagg
//
LOCUS       DAOOQX010000504         1623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_98205_length_1623_cov_3.197066, whole genome shotgun sequence.
ACCESSION   DAOOQX010000504 DAOOQX010000000
VERSION     DAOOQX010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98205_length_1623_cov_3.197066"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PLX53_07890"
     CDS             complement(<1..276)
                     /locus_tag="PLX53_07890"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOY73037.1"
                     /translation="MEAIKIQGTSQYPTIVLDSEAGRFEFSGNSLPEDPKAFYEPILS
                     WLDAYAQNPNPETVVRFKMVYYNTPSSKQIFQIFKKFEKMAASGHKVS"
     gene            complement(432..1334)
                     /locus_tag="PLX53_07895"
     CDS             complement(432..1334)
                     /locus_tag="PLX53_07895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY73038.1"
                     /translation="MIQLLTPESWPEYELIDSGDGEKLERIGSQMVARPEPQALWRKQ
                     LSNEEWVKLADAYFVKSKKTKPTENEWGEWRLKSGAKEQWVVSYNQSGLAFRLRLGLT
                     SFKHVGIFPEQAVSWNYIYNTIKQLDLVETKVLNLFAYTGASSLAARAAEAKVTHVDS
                     VKQVVSWANENMLLSGMDNIRWIVDDALKFVSREVRRGNLYNGIILDPPAYGRGPDGE
                     KWTVEENLYQLLELCKQVMEPKNNFLVLNLYSMGFSPLIAENLIRDIFGVPSYEVGEL
                     FVSDRAGRKLPLGIFVRFRSGSNF"
BASE COUNT          436 a          379 c          317 g          491 t
ORIGIN      
        1 cgaaacctta tgccccgaag cagccatttt ttcaaatttc ttaaaaatct ggaaaatctg
       61 ctttgagctg ggggtattat agtaaaccat cttaaagcga acaactgttt cgggattggg
      121 gttctgggcg tatgcgtcga gccagctaag aattggctcg taaaacgctt ttggatcctc
      181 gggcaaagag ttccctgaga attcaaacct tcctgcttca ctgtcgagca caatagtagg
      241 atattggctg gttccctgta tttttattgc ttccataagc tctccaattt taaaacctaa
      301 tcttgttgga agtacaattg ataatcaaat atatacaaaa tcttgacaaa ggtaataaaa
      361 acaccttact aaattcacca tcattttttt taacaccttt aaaatgggct aacaattaac
      421 caatcagcaa gttaaaaatt actaccagag cgaaatctga caaaaattcc aagcggcagc
      481 ttgcggcctg ccctgtcgga aacaaaaagt tcgcccacct catagctggg aacgccgaaa
      541 atgtcccgta tcaggttttc ggctattaag ggtgaaaagc ccattgagta taggttgaga
      601 acaaggaagt tgtttttagg ttccattacc tgcttgcaca gctcgagaag ttggtagagg
      661 ttctcctcta ctgtccactt ctctccatcg gggcccctgc cgtaggctgg aggatcgagt
      721 ataattccgt tgtagaggtt accccgcctg acttcacggc ttacaaattt caaggcatca
      781 tccacaatcc aacggatgtt atccattcct gaaagtagca tattctcatt ggcccatgaa
      841 acaacctgct ttaccgagtc aacgtgggtt acttttgctt ctgctgctct ggcagccagc
      901 gacgatgccc ccgtataggc aaataggttt agcaccttgg tttcaaccaa atcgagctgc
      961 ttaatagtgt tgtagatata attccagcta accgcctgct cagggaaaat gcctacgtgt
     1021 ttaaacgagg ttaatcccaa ccgtaggcga aatgccaggc cgctctggtt atatgaaacc
     1081 acccattgct cttttgcccc cgactttagg cgccattcac cccactcatt ttcggtcggt
     1141 ttggtttttt tgcttttaac aaaatacgca tcggcaagct tgacccattc ttcatttgat
     1201 aattgcttcc tccataacgc ttgtggttcg ggcctggcaa ccatttgcga cccaattctc
     1261 tccagctttt caccatcacc actgtcaata agctcgtact ctggccagct ttcgggagtt
     1321 aaaagttgaa tcatctgcaa tctttttaag ctgtggcggg tatatgcttt agcacttccc
     1381 gaatcagaaa ctttatcgaa aatacctgtt ttttcagttt tatgcagatt ttttaagaat
     1441 tttaatcagt ataaaatgtt taggtttctg attacaaata cattatgtta ttccttatag
     1501 gtatcgaggt aaagcgtatg gaacgccaat attttggctt cgccaaaccc gtaagtaacg
     1561 cccggttgtt caagcgtatt tatacaagcc gcccttcatt ggtgtttcat ttcttccaca
     1621 ata
//
LOCUS       DAOOQX010000505         1620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_98470_length_1620_cov_3.370607, whole genome shotgun sequence.
ACCESSION   DAOOQX010000505 DAOOQX010000000
VERSION     DAOOQX010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98470_length_1620_cov_3.370607"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..488
                     /locus_tag="PLX53_07900"
     CDS             33..488
                     /locus_tag="PLX53_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73039.1"
                     /translation="MAFIHKKGHIGVVSRSGTLTYEAVDQLTKLGLGQSTGIGIGGDP
                     IIGTTTLDAIKLFMNDPDTHGIVMIGEIGGSMETEAAQWVKIHGTKPVVGFIAGQTAP
                     KGRKMGHAGAIIGGTADTAEAKMNTMRECGIHVVESPANIGRAMKDALK"
     gene            complement(501..>1620)
                     /locus_tag="PLX53_07905"
     CDS             complement(501..>1620)
                     /locus_tag="PLX53_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOY73040.1"
                     /translation="SPKDNPFDHKELYEVLDLCLMCKGCKSECPSSVDMTKLKTEFLQ
                     HWYDIHGIPFRNKMVAYITQIYRLGAIFPKFTNLLLSSKVTSFAIKNMLGFAQSRALP
                     KLSSITLRRWQAKQKNSNKYPNGIVYLLPDEFTNFNESHIGVKAAELLNYLGYEVRIP
                     DILESGRTYISKGLIRTAKRIANNNIDKLKGIIGEDTPLVGIEPSAILSFRDEYPDLA
                     DDRNTKASKELAMNSLLFEEFFMREASKGKIAKEHFKQGNLRIKLHGHCQQKAVASTQ
                     ATKAMLSFPEGYEVSEIPSGCCGMAGSFGFEKEHYELSMKIGELVLFPEIRKSSETDV
                     IVAPGTSCRQQIYAGTGRYALHPIEVMWQAANISSQRS"
BASE COUNT          416 a          354 c          389 g          461 t
ORIGIN      
        1 gttagggtgc ccgaacggga tactacaccg atatggcctt tattcataaa aaaggccata
       61 tcggtgtagt atcccgttcg ggcaccctaa cctacgaggc tgttgaccag cttaccaagc
      121 tgggattggg gcaaagtaca ggaattggta ttggcggtga tcccataatc ggcactacaa
      181 ccctggatgc aattaagcta ttcatgaacg accctgacac tcatggcata gtgatgatcg
      241 gcgaaattgg aggaagcatg gaaaccgagg cggcacaatg ggtaaaaatt catggaacta
      301 agcctgtggt tggctttatt gcggggcaaa ctgcgccaaa aggaaggaaa atggggcatg
      361 ccggtgcaat tattggtgga actgccgata ctgccgaagc taaaatgaat actatgcgtg
      421 aatgcggcat tcatgttgtg gaatcgcctg ccaacattgg ccgggcaatg aaggatgcac
      481 ttaaatagtg gggtggtagc ctatgaacgc tggctgctaa tgttggcagc ttgccacatc
      541 acctctatgg gatgaagtgc ataacgacct gttccagcat aaatttgctg cctgcagcta
      601 gtcccgggtg ctactattac atcggtttct gaagacttac gaatttccgg gaagagtacg
      661 agttcgccta tcttcattga aagttcgtag tgctcctttt caaatccaaa ggaaccagcc
      721 attccgcagc aaccgcttgg tatttcgctt acttcatacc cttcagggaa tgatagcatg
      781 gcttttgttg cctgggtgga agcaaccgct ttttgctggc aatggccatg aagtttaatt
      841 ctcaaatttc cctgcttaaa gtgctcttta gctatcttcc ctttgctcgc ctctcgcata
      901 aagaattcct cgaatagcag ggaattcatg gccagctcct tcgaagcctt tgtattacgg
      961 tcgtcggcca agtcggggta ctcatccctg aagctaagta tggctgaagg ctcaatgcct
     1021 accagcggtg tgtcttcacc tattattccc ttaagcttat caatattatt gttggctatg
     1081 cgcttggctg ttctgataag gccttttgaa atgtaagttc tgccactctc aagtatatcg
     1141 ggaatacgta cttcgtagcc aagatagttt aacagctcag ctgcttttac tccaatatgc
     1201 gactcgttga agtttgtaaa ctcatcgggc aacaggtaaa ctataccgtt agggtattta
     1261 tttgagttct tctgttttgc ctgccatctg cgcagagtta tactacttag tttaggcagt
     1321 gctctgcttt gagcaaaacc taacatgttc ttgatagcga atgaggttac cttgctggat
     1381 aacaatagat tggtaaattt gggaaaaatt gcccccaaac gataaatttg ggtgatatag
     1441 gcaaccattt tgtttcggaa cggaatgcca tgaatgtcgt accagtgctg aaggaattca
     1501 gttttaagct ttgtcatgtc aacacttgaa gggcattcgc ttttacaacc cttgcacatc
     1561 aggcagaggt ctaaaacttc gtacagctcc ttgtgatcaa aaggattgtc tttagggctg
//
LOCUS       DAOOQX010000506         1619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_98588_length_1619_cov_3.347826, whole genome shotgun sequence.
ACCESSION   DAOOQX010000506 DAOOQX010000000
VERSION     DAOOQX010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98588_length_1619_cov_3.347826"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PLX53_07910"
     CDS             complement(<1..454)
                     /locus_tag="PLX53_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73041.1"
                     /translation="MNKRNVVIIGAAGRDFHNFNTFFRNKEEYNVVAFTAAQIPDIDG
                     RKYPAELAGKLYPNGIPIVSEEELPRLIKEKNVHDCVFSYSDVKYQNVMRISSIVNAA
                     GANFMLLGPNETMLKSTKPVIAVVATRTGCGKSQTSRKVIEYLMGLGLK"
     gene            complement(847..>1619)
                     /locus_tag="PLX53_07915"
     CDS             complement(847..>1619)
                     /locus_tag="PLX53_07915"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOY73042.1"
                     /translation="VYSNAETPTVILQIKGEVIPHVKTPDEIFNISIGGKLYFEKTHI
                     PFSRLFPGKIYTDTLKFMNKSSEVVKVAANAGGLNFLTVRVEPQSIKPKQVGNIIVTY
                     DPQKRNDWGFVMDRFMLSINDSLITNTPISITGSIEEDYSALSASELEKAPRVEFNTT
                     SYDFGEKAEGEPVEYSFTIKNTGKSDLQIRKVKASCGCTTVQPSKNILKPGESTEIKA
                     LFRTNGYSGRQSKSITVITNDPKSSTTVLRLSGNVVKK"
BASE COUNT          429 a          345 c          347 g          498 t
ORIGIN      
        1 ccttcaggcc aagccccatc aggtactcta taacctttcg ggatgtttgc gatttaccgc
       61 atccggttcg ggttgctacc acagcaataa ccggcttagt gctcttcagc atggtctcat
      121 tgggccccaa cagcataaag tttgcacctg ctgcgttcac aattgagctg atccgcatta
      181 cattctgata cttgacatcg ctgtaggaga aaacgcaatc atgaacgttc ttctccttga
      241 tcaggcgggg tagctcctcc tccgatacta tgggaattcc atttgggtaa agctttcctg
      301 ccagctctgc aggatatttt ctaccatcaa tatcgggaat ctgagctgct gtaaaggcta
      361 caacgttgta ctcctccttg ttgcgaaaga aagtgttgaa gttgtgaaaa tctcttccgg
      421 cagcgccaat aattactaca ttacgtttat tcataaagcc tcctttaaag tgtttactaa
      481 atttaaggtt tgttaaaatc cataacgctc acaaaattaa gtgcattaag aaataattat
      541 ttttcgttgg aatttttttt taggttcgta tttttcacaa aaaatgaaaa ttcaagtcaa
      601 atgtctgtta ttcttatact tagtgatgtt ttctgaaaaa catgttaaga aacatgtaat
      661 ggcaggcttg ttcggtagcc ccttgccagc agcaaatgta agctggcgaa gtgatgccgt
      721 gcatggagcg aacaacgggg aaggtcaaaa taattgcgag cagatggaat gaatggtggc
      781 ttaatctcac gaaaaagggc tgcaagttgc agcccttttt ccgcatcatt acatcaatgc
      841 agcttactat ttcttaacaa catttcccga taaacgcaga accgtggttg aacttttagg
      901 atcgttggtt atcacggtaa tagatttact ctgcctaccc gaatacccat tagtcctgaa
      961 cagggcttta atctcggttg actcgcctgg tttaagaatg ttttttgaag gttgaacggt
     1021 tgtgcagccg cagctggctt taaccttacg aatctgcaga tcgcttttcc ctgtgttctt
     1081 aatggtaaag ctgtactcta cgggttctcc ctcagccttc tcgccaaaat cgtaggaggt
     1141 tgtgttaaat tctaccctgg gagccttttc cagctcactg gcgctgaggg cggagtaatc
     1201 ctcctcaatg ctgccggtaa tcgatattgg ggtgtttgta atcagggaat cgttgataga
     1261 aagcataaac ctgtccatta caaaacccca gtcgtttcgt ttttgaggat cgtaggtaac
     1321 tataatattt ccaacttgtt taggcttaat actctggggc tcaaccctta cggttaagaa
     1381 atttaaccca cctgcattgg cagcaacctt aaccacctcg cttgatttgt tcataaattt
     1441 gagggtgtcg gtataaattt tcccggggaa tagccggcta aaaggaatat gggttttttc
     1501 aaagtatagc ttaccgccga tggaaatgtt gaatatctca tcgggagttt taacatgggg
     1561 aataacctcc cctttaattt gtagaataac cgtaggtgtt tcggcatttg agtaaacag
//
LOCUS       DAOOQX010000507         1616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_98883_length_1616_cov_3.377963, whole genome shotgun sequence.
ACCESSION   DAOOQX010000507 DAOOQX010000000
VERSION     DAOOQX010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98883_length_1616_cov_3.377963"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="PLX53_07920"
     CDS             complement(<1..1232)
                     /locus_tag="PLX53_07920"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOY73043.1"
                     /translation="MTLSKLNRKEPPERRAVRKIELPEPSISTLPNGAKLAVINAGTQ
                     DIVKMEVIFNGGTRYQKKILSSQAAVSLMCEGTTTRNSNQLAEQLDFLGSFLQHSFDR
                     DFASVSLFTTRKHFAASLEVVADILTNPTYPENELEIFRKKGKQSLTVELEKVETLAK
                     QGFFQCLFGPDHPYGSFATPDDFDKLSQPDVADFHKNFHNLSNSFIILAGRVDEHEVK
                     TVETLLGANSPGRRLGALNNSLPAAAQTPREKFTYKENAIQSAVRIGKQMVSRNHPDY
                     IGLMVLNTILGGYFGSRLMQNLREDKGFTYGISSILIPYRETSVFMIGTEVGREVTKP
                     AVEEIYNELDRLVRVPVPAEELELVKSQMTGDLLRTLDGPLAIADSIAELYQYNNMNL
                     NFLDQTIETINTITSNKL"
     gene            complement(1247..>1616)
                     /locus_tag="PLX53_07925"
     CDS             complement(1247..>1616)
                     /locus_tag="PLX53_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOY73044.1"
                     /translation="NAFVTGDIDEGLLVVTGQLFPSTSFSDADESITSELEELKIKPV
                     SDYELEKVKNRFESNFVFEETSILNKAINLSFYEQLGNAGYINQEVEKYRAVSTNRIM
                     EVAREILSVNNCSTLYYQKN"
BASE COUNT          395 a          353 c          382 g          486 t
ORIGIN      
        1 tgcagcttgt tggaggtgat ggtgttaatg gtttcaatag tttggtcaag gaaatttaaa
       61 ttcatgttgt tgtactggta gagttcggca atgctgtcgg caatagctag tgggccatcc
      121 agggttcgaa gcagatctcc agtcatctgg ctttttacca gttccagctc ttcagcgggt
      181 accggaacac gaaccagcct gtcaagttca ttgtaaatct cctcaacggc aggtttggta
      241 acttccctgc ctacctctgt gccaatcata aatacggatg tttccctgta gggaatcaga
      301 atggatgaaa tgccgtaagt gaatcccttg tcctctcgaa ggttctgcat aagccttgag
      361 ccaaagtatc cacccagaat cgtgtttaga accataagcc caatgtaatc gggatgattt
      421 cggctaacca tttgttttcc aattcttact gccgactgaa tggcattttc tttatacgtg
      481 aatttttctc tgggggtttg tgcagctgcg ggtaaagaat tgttcaaagc ccctaaacgt
      541 cttcccggtg aattggctcc cagaagtgtt tcaacggtct tcacttcatg ctcatcaact
      601 cttccggcca gaatgatgaa gctgttggaa aggttgtgga agttcttatg aaaatcggcc
      661 acatcaggct gggatagctt gtcgaaatcg tccggggtgg caaagctgcc gtagggatgg
      721 tcgggaccaa aaagacactg aaagaaaccc tgcttagcca gtgtttcaac tttttccagc
      781 tccacagtaa ggctctgttt tcccttcttt ctaaaaattt cgagttcatt ctccgggtat
      841 gtgggattgg ttaggatgtc agctactacc tcgagtgaag cggcaaagtg tttacgggtg
      901 gtaaatagcg ataccgacgc aaagtcgcga tcaaatgaat gttgcagaaa actacccaaa
      961 aagtcgagct gttctgcaag ctggtttgaa tttcgggtgg ttgtcccctc gcacatcaac
     1021 gaaacggctg cctgtgacga tagaattttt ttctggtatc gggtgccacc attaaaaata
     1081 acttccattt tgacaatatc ctgggtaccc gcatttatta ccgccagttt agcaccgttg
     1141 ggcagggtgc tgatggacgg ttcaggtagt tctattttcc taacagctct tctttctgga
     1201 ggctctttcc tgtttagctt gctgagggtc atttatattt tgatgtttag ttcttctgat
     1261 agtatagggt ggagcaatta tttacggaaa gtatctcccg cgcaacctcc ataatccggt
     1321 ttgtgctaac cgctctatat ttttctacct cctgatttat atagccggca ttgccaagtt
     1381 gctcgtagaa cgagaggtta atggccttgt tcagaatact ggtttcttca aaaacgaagt
     1441 tggattcaaa gcggttctta accttttcaa gctcatagtc agaaacaggc ttgattttga
     1501 gttcttccag ctcggaggtt atgctttcat ctgcatcgct aaaggatgta gagggaaaga
     1561 gttgcccggt aacaaccagc agcccctcgt caatatcgcc ggtaacaaag gcatta
//
LOCUS       DAOOQX010000508         1615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_98988_length_1615_cov_3.437821, whole genome shotgun sequence.
ACCESSION   DAOOQX010000508 DAOOQX010000000
VERSION     DAOOQX010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98988_length_1615_cov_3.437821"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLX53_07930"
     CDS             <1..322
                     /locus_tag="PLX53_07930"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HOY73045.1"
                     /translation="ALLRLAYKSQLDVSNYSEARTFRVDTKGTTRLFIGLGRNDGMTP
                     KKISSFIRQEVQIPDNKIDDIVVRDDFSFITVPFVDAEKILKAFANQETDGKPLITKA
                     KPKK"
     gene            337..1527
                     /locus_tag="PLX53_07935"
     CDS             337..1527
                     /locus_tag="PLX53_07935"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOY73046.1"
                     /translation="MVEKFEMIAKTLQGLEGVLADELTELGAEDVKIENRAVSFRGTT
                     ETLYKANFWLRTAIRVLKPVYSFSARTIEEFYGNARKFDWTSLMDLSQKFAVDSVVNS
                     EVFPHSRYIALKFKDAVVDQFRDRYGKRPFVDTKNPQIQFHVHISGDSCTILLDSSGE
                     SLHRRGYRTAQDIAPINEVLAAGLIKLSGWNMDSTFIDPMCGSGTIVIEAALMAYGIA
                     PGVFRRQFGFENWLDFDEKLLQSIYNDDSMEREFTHPIIGRDISRQTVESALLNVKHA
                     GLQKKVDIEVGSIFDFQPPADSQGIVVTNPPYGERLRREQIETFYKQLGDCFKQKYEG
                     YNVWMISSNMDALKNFGLRPSKRYTLYNGALECKFQRYSMFAGSVKAKYQGKGERIKS
                     KDRE"
BASE COUNT          430 a          376 c          388 g          421 t
ORIGIN      
        1 tgccctgttg cgcctagcct acaaatcgca actcgatgtc tccaactact ccgaggctcg
       61 tacttttagg gtggatacta aaggcacaac ccgcttgttt attggacttg gtcgtaatga
      121 tggcatgacc ccaaaaaaga tttcttcctt catcaggcag gaggttcaaa tccccgataa
      181 taagattgac gacattgttg ttcgcgatga tttttccttt attaccgtgc cctttgtgga
      241 tgccgaaaaa attctgaagg cctttgccaa tcaggaaaca gatggtaaac cccttataac
      301 aaaagccaaa ccaaaaaaat aagcgagtag gcctttatgg tggaaaagtt tgaaatgatt
      361 gccaaaaccc tccaggggct ggagggtgtt cttgccgatg aactcaccga acttggtgct
      421 gaggatgtga agatcgaaaa tagggccgtg agcttccggg gaacaacgga aaccctttac
      481 aaggctaact tctggttacg aacagccatt cgggtgctta agcctgttta ctcatttagc
      541 gcccgaacca ttgaggagtt ctacggaaat gcccgtaagt tcgactggac ttcactgatg
      601 gacttatcgc aaaaatttgc agttgacagc gtggtcaact ctgaggtttt cccacattca
      661 cgttacattg cccttaaatt taaagatgct gtggttgatc agttccgcga tcgctacgga
      721 aaacgtccgt ttgttgatac caaaaatccc caaatacagt tccacgtcca catttcgggc
      781 gatagctgca ccatcctgct cgattcctca ggggaatcgt tgcaccgtcg cggctaccga
      841 actgctcagg atattgcccc tatcaacgag gtgctggcag cagggctaat caaactttcg
      901 ggttggaata tggattcaac ctttattgac ccaatgtgcg gctctggaac tattgttatt
      961 gaggcagccc ttatggcgta tggaatagca cccggtgttt ttcgccggca gtttggtttt
     1021 gaaaactggc tcgattttga tgagaaactt ctccagtcaa tctataacga cgatagcatg
     1081 gagcgggagt ttacacatcc aattattgga cgtgatatct cgcgccagac cgttgaatcg
     1141 gcgttgctca acgttaagca tgcaggtttg cagaaaaaag ttgacattga agtaggctcc
     1201 atcttcgact ttcagcctcc tgccgattct cagggcattg ttgttaccaa tccaccttac
     1261 ggcgaaaggc ttcgacgtga gcaaatcgaa actttctaca agcagctggg cgactgcttt
     1321 aaacagaaat atgaggggta caacgtttgg atgatcagct cgaacatgga tgccctgaaa
     1381 aattttggtt tgcgaccatc aaaacgctac accctctata acggcgctct tgaatgtaag
     1441 ttccaacgct actccatgtt tgccggtagt gtaaaggcga agtaccaggg taaaggggaa
     1501 aggataaaga gtaaggatag agaataagat aaacttgtcc gcctaccggc ggaacttgtc
     1561 cggctcccga cggattcacc cttcgacagg tgttacagtg agtagttcgg ctggc
//
LOCUS       DAOOQX010000509         1610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_99549_length_1610_cov_1.668810, whole genome shotgun sequence.
ACCESSION   DAOOQX010000509 DAOOQX010000000
VERSION     DAOOQX010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99549_length_1610_cov_1.668810"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1610
                     /locus_tag="PLX53_07940"
     CDS             <1..>1610
                     /locus_tag="PLX53_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73047.1"
                     /translation="NFLSATRNQSMLLYGPTRFYRIAISKGLSSTYELYMWASSSAYF
                     ELFGYANEGHPDEKQLLVNNNSLGLAYGTVRVGANITVPRLNGGGNYNVSENAILWVD
                     GGTVTKPSGTAVVVYGKVKVSAGTFNANINSGITTRLNGVFESTGGTTNLGQFRTSVY
                     GTQHIGGFIQSGGNVTVDQAYGSSSDYYLFSLSYEGNIFTLTGGTLHIKGTNAKGSVF
                     INSDPVNQNVSTNATLILESTSTSPFRITSRAPFPNVTMSRSGSGTRTLVLEGGIVGS
                     NPGNQAELPALPLVTKGTLTINDNVVFDAKGQDVTIGRSYSIGSGANYIAASNTTTFQ
                     GASQNYIIAINAAATTKYFHNLTINNNGYTGTLSVSDITVGNNLTITEGSLATGAKTV
                     TVRGNIINSDTIKSTSGGILITNRGIVNLVTVTNGGSYTSVPTVTIAAPGGSGIQATA
                     VAIFNGTPSGSNPLPVARIAITNTGSGYTSAPTVSFSAGAATATASISTTHELGGNGN
                     GVFGSLELNEPHPSEASEKVEVTYLSAN"
BASE COUNT          442 a          446 c          384 g          338 t
ORIGIN      
        1 caacttcctg agcgccacgc gtaaccagag catgttgcta tacggcccaa cccgtttcta
       61 ccgtatagca atcagcaagg gactctccag cacctacgaa ctctacatgt gggcatccag
      121 ctcagcctac ttcgagcttt ttggctatgc caacgagggg caccccgacg aaaagcagct
      181 gttggttaac aacaactcgt taggcctggc ttacggaacc gtaagggttg gggcaaatat
      241 aacggtgccc cgtttgaatg gaggaggcaa ctacaacgtt tcggaaaatg ccatcctttg
      301 ggttgatggc ggcacagtta cgaaaccaag cggaaccgct gtggtagttt atggcaaggt
      361 aaaggtttcg gcaggaacgt tcaacgctaa cataaacagc ggaatcacca ccaggttaaa
      421 cggcgtgttt gagtccaccg gtggcaccac caacctgggc cagttcagaa catccgttta
      481 tggaacccag cacattggcg gattcattca gtccggaggc aatgtaaccg tagaccaggc
      541 ctacggttca tcttctgact actatctctt ctcgctatcc tatgagggaa acatattcac
      601 cctgaccggt ggaacgctgc acatcaaggg aacaaacgcc aagggttcgg tttttatcaa
      661 ttccgaccct gtaaaccaga atgttagcac caacgccacc ctgattttag aatccacgag
      721 cacttcccct ttccgcatta cctcacgagc tccctttcca aatgtaacca tgagccgttc
      781 cggctcagga acaagaacct tggtgcttga ggggggaatt gtaggaagca atcccggtaa
      841 ccaagctgag ctgccggccc tacccttggt aacaaaggga actctgacaa taaacgacaa
      901 cgtagtattc gatgccaagg ggcaagacgt aactattggg cggagctaca gcattggttc
      961 gggagccaac tacattgcag caagtaacac gacaaccttc cagggggcat cccagaacta
     1021 cattattgct ataaatgcgg ctgcaactac aaagtacttc cataatctca caataaacaa
     1081 caatggttac accggaaccc tttcggtaag cgacatcaca gttggtaaca atttgaccat
     1141 taccgaaggg tcgcttgcaa ccggcgctaa aacagtaact gtgcgcggca atatcattaa
     1201 ctccgacacg attaaaagca catcaggagg tattcttatc acaaaccgtg gtattgtgaa
     1261 tctggttacg gttactaatg ggggcagcta cacctccgtt ccaacggtaa ccattgcagc
     1321 tcccggcgga tcgggaatcc aggccactgc tgtggccata ttcaacggaa ctccctctgg
     1381 atctaacccg ctcccagtgg ccagaatagc cataaccaac accggtagcg ggtacacctc
     1441 agccccaacg gtgagcttca gtgctggggc agccactgcc acggcatcca tctcaacaac
     1501 ccatgagctt ggtggcaacg gcaacggcgt atttggcagc ctggagctga acgaacccca
     1561 cccaagcgaa gcatccgaaa aggtggaggt aacatacctg tctgcaaatc
//
LOCUS       DAOOQX010000510         1607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_99826_length_1607_cov_2.446521, whole genome shotgun sequence.
ACCESSION   DAOOQX010000510 DAOOQX010000000
VERSION     DAOOQX010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99826_length_1607_cov_2.446521"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PLX53_07945"
     CDS             <1..688
                     /locus_tag="PLX53_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73048.1"
                     /translation="LLAETIMLLAQSDEDPLIFIDGDAFPVTPLDDFIGMQLERYPLA
                     AVQRLENVGDIQPHPSFCLTTVGFWKSISGDWLKGYEWTNSRGVKRTDVGGNLLKILN
                     DLGVEWKPLLRSGQLSEHPLMFGIYENAIYHHGAGFRQPLSMYDRLKPTSHVVKNKAV
                     AGGIDLVLDKLPLKNRFFLQRHLGLKKNILRQNVELSEAIYHDFASIISKGEAYDFIS
                     KSRRFPVTGS"
     gene            complement(713..1234)
                     /locus_tag="PLX53_07950"
     CDS             complement(713..1234)
                     /locus_tag="PLX53_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOY73049.1"
                     /translation="MDTLQDFVPVSPYELNDNFFDAVDNGWMLITAGDEQKFNTMTAS
                     WGSFGILWNMPIAQVFIRPQRYTFQFVEQHSYYTLSFFDEKWRKALNFCGAKSGRKFD
                     KPKETGLVPAATGHGIAFQQARMVLECRKLYADNLKPEQFIDRDIIRKNYPKSDFHRF
                     FIGEIVNVYLARG"
     gene            complement(1245..>1607)
                     /locus_tag="PLX53_07955"
     CDS             complement(1245..>1607)
                     /locus_tag="PLX53_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOY73050.1"
                     /translation="GLITGIAASLSMAASQYISVSTENSGQNALKSSIYTGIAYVITV
                     IILIAPYLILQSYFAALALTLTFAILIILVFNYYISVAKDLNFKRRFSEMAIISLGVA
                     ALTFGIGFLIRKYLGVDI"
BASE COUNT          415 a          374 c          401 g          417 t
ORIGIN      
        1 cctgctggcc gaaaccatca tgctgctggc acaatcggac gaagacccgc ttatctttat
       61 cgatggcgat gcctttccgg taacacctct tgatgatttt atcggcatgc agcttgagcg
      121 ctaccctctg gctgcagtgc aacgcttgga aaacgtgggc gatatccagc cccatccttc
      181 attttgcctc accacggtcg ggttttggaa aagcataagt ggagactggc ttaagggata
      241 cgagtggact aactccaggg gagttaagcg caccgacgtg ggtggtaacc tccttaaaat
      301 actgaacgat ttaggtgttg agtggaaacc gctactgcga agcgggcagc tgagcgagca
      361 ccccctgatg tttggcatat acgaaaatgc catttaccac cacggggcgg ggtttaggca
      421 acccctctcc atgtacgaca ggcttaaacc tacatcgcac gtagtgaaaa acaaggctgt
      481 tgccggtgga attgacctcg tccttgataa gctacccctg aagaatcggt ttttcctaca
      541 gcgtcactta ggcctgaaaa agaatattct caggcagaat gttgagctaa gcgaggcaat
      601 ctaccatgat tttgcttcta tcatatccaa aggcgaagcc tacgatttta tttccaaatc
      661 gaggcgtttt ccggttaccg gcagctgaat tttttagaat ggacagagga agttagcctc
      721 ttgccaggta aacattcaca atttcgccga tgaaaaaccg gtgaaagtcg cttttggggt
      781 agtttttcct gatgatatcc ctgtctatga attgctcagg tttcaggtta tcggcataca
      841 gctttctgca ttcaagcacc atgcgggcct gctgaaaggc tataccatgc ccggtggctg
      901 ccggtaccaa tcctgtttcc tttggcttgt cgaactttcg gcccgatttt gccccgcaaa
      961 agttgagtgc cttgcgccat ttttcatcaa aaaagctaag ggtgtagtag ctgtgttgct
     1021 caacaaactg gaaggtgtag cgttggggtc ggataaaaac ctgtgcaatt ggcatattcc
     1081 atagtatgcc gaagctaccc cacgaagccg tcatggtgtt aaatttctgc tcgtcgcctg
     1141 ccgtgattag catccagccg ttatcaacag catcgaagaa gttatcattc agctcgtagg
     1201 gtgaaaccgg aacaaaatcc tgaagtgtgt ccatgcaatc cgaattaaat atcaactccc
     1261 aaatacttgc gaataaggaa tcctattcca aaggtgaggg cagctactcc caggctgatt
     1321 atggccattt cgctgaagcg ccttttgaaa ttcaaatcct tagccaccga tatgtagtag
     1381 ttgaacacca gtataatcag gatggcaaag gttagggtaa gggcaagcgc agcgaagtag
     1441 gattgtagta tgaggtaggg agctatcagg atgataaccg ttatgacgta tgcaatgccg
     1501 gtgtaaattg atgatttcag ggcattttgg ccgctattct cagttgatac cgagatgtac
     1561 tgggaggccg ccattgatag cgaggcagca ataccggtta tcaatcc
//
LOCUS       DAOOQX010000511         1604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_100086_length_1604_cov_2.989025, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000511 DAOOQX010000000
VERSION     DAOOQX010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100086_length_1604_cov_2.989025"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1604
                     /locus_tag="PLX53_07960"
     CDS             <1..>1604
                     /locus_tag="PLX53_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOY73051.1"
                     /translation="FVVPFTTIFTPAIGAPSLSVTLQEGAPMAGVNIVVKGTTNGTNT
                     DVDGKYSISTPANAILQFSFIGMKSVEEAVGGRSIIDVVLESESMDISEVVVIGYGTS
                     RKKDITTAVSVVSTEDFDKRPVTNVAQAIQGKAAGVQVVLPSGKPGGDAFIRVRGATS
                     VLAGNDPLFVIDGVPTTSMQALNPQDIETVQVLKDASSAAIYGARAANGVVIITTKHA
                     KAGVASVSVNSYMGFSNLANSLDVLNTEQYRELMDEVKGPGTVPTSQTNYTNWIDETF
                     STGQIQNYQVSVSNGTEKMKYYFSAGFSDEKGIVKPAENKKYTIRTNINSDLFKWLKF
                     GTNISFIHNWYNDVSDNANSGRAGTILSAINTPPFLHIWDPNNPEQYEVNPFQNSWEN
                     PLAYASRINQSKTERVLANANTEITFLKDFKLKSNFGIDGYIYQRDLYIDPVKTVYGR
                     TENGIGEADKSNSLVWLIENVLSYEKTFGNHNLSVQAGQSAQSSRWDQSYISAKDFPK
                     NSAVTTINAANQITYAASSASEWAIA"
BASE COUNT          450 a          379 c          380 g          395 t
ORIGIN      
        1 cattcgtggt tccctttaca acaatattta cacctgccat tggcgctccc tccttatcgg
       61 taacccttca ggagggagcg ccaatggcag gtgtaaatat tgttgtaaag ggaaccacga
      121 atgggacaaa taccgacgtg gacggaaagt atagcataag cacaccggcg aatgctattc
      181 tacagttctc ctttattggt atgaagagcg ttgaggaggc tgttggcggt cgttccatta
      241 ttgatgtagt tctcgaaagc gaaagcatgg acatttctga ggtggtggtt ataggatatg
      301 gcaccagccg gaaaaaggac attaccaccg ctgtttcggt ggtttcgact gaagattttg
      361 acaagcgacc cgtaaccaac gtggcacagg caatacaggg aaaggccgca ggagttcagg
      421 ttgtgcttcc ttcgggtaaa cccggagggg atgcatttat cagggttaga ggtgcaacat
      481 cggtgctggc cggtaacgac cccctgtttg taattgatgg agttcctact accagcatgc
      541 aagcacttaa cccgcaggat attgaaacgg ttcaggtgct gaaggatgcc tcttctgccg
      601 caatttatgg cgcacgtgct gcaaatggtg tggtaattat taccaccaaa catgcaaagg
      661 ccggtgttgc ttcagtttcg gttaatagct acatgggctt ctcaaacctg gctaactcac
      721 tggatgtgct caacaccgag cagtaccggg agttaatgga cgaagtaaag ggaccaggaa
      781 ctgttccgac ctcccagact aactacacca attggattga tgaaaccttc agcaccggac
      841 aaattcaaaa ctaccaggtt tcggtttcca acggtaccga gaagatgaag tactactttt
      901 cggctggttt ttccgatgaa aagggtattg ttaagcccgc tgaaaataaa aagtacacca
      961 ttagaaccaa catcaacagc gacctgttca agtggcttaa atttggaacc aacatcagct
     1021 ttattcataa ctggtacaac gatgtaagcg ataatgcaaa ctccggaagg gcaggtacca
     1081 tccttagcgc catcaacacc ccacccttcc ttcatatctg ggatccgaat aaccccgaac
     1141 agtacgaggt taacccattc cagaattcat gggaaaaccc tctggcttat gcttcgcgta
     1201 tcaaccagtc gaaaactgag cgggtgctgg ccaacgcaaa taccgagatt accttcttaa
     1261 aggattttaa gctgaaatca aatttcggga ttgatgggta tatttaccag cgcgatctct
     1321 acatcgaccc tgttaaaacc gtttatggac gtacggaaaa cggaattggc gaggccgata
     1381 agtcaaactc cttggtttgg ttgatagaga acgtgctatc ctacgaaaag acctttggca
     1441 accacaacct ctctgttcag gcaggtcagt cggcccaatc gtcacgttgg gaccaatcct
     1501 acatatccgc taaagacttt ccaaagaatt ctgctgtaac taccattaat gctgctaacc
     1561 agattaccta tgctgcatca agcgcttcgg aatgggcaat tgca
//
LOCUS       DAOOQX010000512         1604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_100094_length_1604_cov_2.797289, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000512 DAOOQX010000000
VERSION     DAOOQX010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100094_length_1604_cov_2.797289"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1386
                     /locus_tag="PLX53_07965"
     CDS             214..1386
                     /locus_tag="PLX53_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOY73052.1"
                     /translation="MDELLSFLTPTAIKGVNFSSQNSRMLQQQVVPLNKTNYKHLKKN
                     DIVVIGVNESRNSPNPSIKNSPETIRKYLYGLSTIKLAGKIYDAGDLVETSTPLKTYE
                     ALNRVVEILFQTGAATVVLGGTQELTYCLYEGVKSNKSLVTISIIDCLIDADEDLSDF
                     HSRNYVNRLLGEKLRSLFELSIVGYQGYYVGGDMVNLFQKNNHELLRLGYVRSSYREV
                     EPTLRNADIVSFDMEAIRACDSPANAIPSPNGLYAEEACQLAHYAGVGERASLFGIFN
                     YNPDTDNTEQSALLAAQLAWHFIEGYSKRKNVPKTLNTPDYLKFIVGTGTSGVDMVFY
                     CDEALDRWWMEIPISPEMGIPTNRIIIPCSHSDYTLASRQELPDRWLRIFQKIQQL"
BASE COUNT          485 a          371 c          319 g          429 t
ORIGIN      
        1 tacatttcat tcaacaagca gaactacaaa atacccaagg acaaagatcc gaaattgctc
       61 accttcgatg actgcatgaa gctcattgac gaacaggctg aaaaaggaag taaaaggaat
      121 tctactgcca aaaaaacttt gcccaagcgg caggggaaaa aaggaaacac aaaatagttt
      181 gccatttggc gccaccatat aacttttaaa accatggacg agctgctgtc atttttaaca
      241 cccacagcca ttaagggggt taactttagc tcccaaaaca gccgtatgct ccagcaacaa
      301 gtagtcccgc ttaacaaaac taactacaag cacctaaaaa aaaatgatat agttgtaatc
      361 ggcgtaaatg aaagccgtaa ctccccaaat ccatctatca aaaactcccc tgaaaccatt
      421 agaaagtacc tttatgggct ttcgaccata aagctagccg gtaaaatata tgatgcaggc
      481 gatctggtgg aaaccagcac ccccttaaaa acctatgagg ccttaaatcg agttgtagaa
      541 attctattcc agaccggagc agcaacagtg gtattgggcg gaacccagga gttaacttac
      601 tgcctttacg aaggcgtaaa gtctaacaaa tcgctggtaa caatttccat tatcgattgt
      661 cttattgatg ccgacgaaga cttgtctgat tttcattcac gtaactatgt caacaggctg
      721 ctgggcgaaa agctgcgctc gcttttcgaa ttaagcattg ttggctacca gggctactat
      781 gttggcggag atatggttaa cctgtttcag aaaaacaatc acgaactttt acggcttggc
      841 tatgtcagga gtagctatcg cgaggttgag ccaacccttc gaaatgccga cattgtaagc
      901 ttcgacatgg aagccataag ggcctgcgac tctcctgcca acgccattcc ctctcctaat
      961 gggctctacg ctgaggaagc ttgtcaactt gcccattatg caggtgttgg agaacgagct
     1021 agccttttcg gaattttcaa ctacaatcct gatacggaca acacagaaca gtcggcctta
     1081 cttgcagccc agctagcatg gcacttcatt gagggttatt caaaaaggaa aaacgttccc
     1141 aaaaccctaa atacacctga ctacctgaag tttatcgtag gaactggcac atcaggagtt
     1201 gacatggttt tctactgcga cgaagcgctc gatcgctggt ggatggagat tcctatttcc
     1261 cctgaaatgg gcattcccac caatagaatt attatcccat gctcccatag cgattacacc
     1321 ctggcaagcc gacaggagct gcccgaccgg tggctccgaa tatttcagaa aatacagcag
     1381 ctctagtgca gctcatcttt tgctttaggg ttaagctgta gctgctattg ttaaacttga
     1441 ccctactttt ctatattttt gaattatacg gtaacccttt tggtcaaaaa tctgtataac
     1501 attctgcctt cggttaaaat tttatcatat ttgttgataa atttgtatgc ctctaatttt
     1561 tttgcttact ttactatcta attgaaaaga acgttgaata tttt
//
LOCUS       DAOOQX010000513         1602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_100317_length_1602_cov_2.692954, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000513 DAOOQX010000000
VERSION     DAOOQX010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100317_length_1602_cov_2.692954"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PLX53_07970"
     CDS             <1..1209
                     /locus_tag="PLX53_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOY73053.1"
                     /translation="RYLFAKKSHNVINIISIISLVGVATGVMALVVVLSVFNGFDTLI
                     ERLYSNIDADIKILPREGKTFSITGEKFDKLAKMNGVAVFAEIFEENALFSYRGKQHI
                     GIIKGVSRNYSELTGLDSMMKDGEFKLWMGSQPMAVVGQGVAYYLNANLAQFDPLFVY
                     IPKRGRASLTMESAFGKKAIMPSGIFSVEQDFDTQYVIAPVEFVRNLLGYDSTMVTAI
                     EVKVAGGVDAESIRSSMEEVLGSDFRVLNRYQQNESLYRTMKSEKFAIGLILTLILVI
                     ASFNIVGSLSMLIIDKRKDVETLRSLGADNGLIQKIFLVEGMLISIGGTIIGTLLGLL
                     ICWLQVHFKLITLEGRGNFIIDAYPVDIQLPDIGAIILVVLLIGYLAARFPVRMITRR
                     ILNEDIRSNE"
     gene            complement(1233..1355)
                     /locus_tag="PLX53_07975"
     CDS             complement(1233..1355)
                     /locus_tag="PLX53_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73054.1"
                     /translation="MLRPILSPRVVTLAFGFVDMGGEAEAGKGNVARGLCLASM"
BASE COUNT          386 a          379 c          422 g          415 t
ORIGIN      
        1 cgttacctct ttgcgaagaa atcgcataat gtcatcaaca tcatctccat catttcgctg
       61 gtaggggtgg caactggggt aatggcgctg gtggttgtgc tttcggtgtt caacgggttc
      121 gacaccctta tcgagcggct ttactcgaat attgatgccg atattaagat tcttccccgt
      181 gagggtaaaa ccttcagcat taccggggaa aaattcgaca agctggcaaa aatgaatgga
      241 gtagccgttt ttgctgagat ttttgaagaa aatgccctat tcagctaccg tggcaagcag
      301 cacataggca ttattaaagg cgtaagccgg aactacagcg agcttaccgg actcgactcc
      361 atgatgaaag acggcgagtt caagctatgg atgggtagcc agcccatggc tgtggtgggg
      421 cagggtgtgg cctattacct caatgccaac ctggcgcaat tcgaccccct ttttgtttac
      481 attcccaaaa gagggagggc atcccttact atggaatcgg cttttgggaa gaaggccatc
      541 atgccgtcgg gaattttctc cgttgagcag gattttgata cgcagtacgt cattgcgccg
      601 gttgagtttg tgcgaaacct gctgggttac gactccacca tggttactgc tattgaagtc
      661 aaagtggccg gcggggtgga tgccgagagc atcaggagta gcatggagga ggtactgggc
      721 agcgatttta gggtgctaaa ccgctaccag cagaacgagt cgctctaccg gaccatgaag
      781 tcggagaagt ttgccatagg gctgatttta actctcatac tggttatagc ctccttcaac
      841 atcgtggggt cgctctccat gcttatcatt gataagcgca aggatgtgga aaccctccgc
      901 agcctgggtg ccgacaacgg cttgatacaa aaaatattct tggttgaggg catgctcatt
      961 tccattggcg gaacaattat cgggacactt ttgggactgc tcatttgctg gcttcaggta
     1021 catttcaagt tgattaccct tgaaggtcga ggcaacttca tcattgatgc ctacccggtt
     1081 gatatccagc tgccggacat aggggcaatt atcctggtgg ttctcctgat tggctacctg
     1141 gctgcccgat tccccgtgcg gatgattacc cgccgcatac tgaatgagga tatccgtagt
     1201 aacgagtagg ctttgggcat tttttgggcg gtttacatgc ttgccaggca cagccctctg
     1261 gccacatttc ccttcccggc ttcagcctct ccgcccatgt ccacaaaacc aaatgccagg
     1321 gtgacgaccc tagggcttag tatgggtcgc agcatttagc caggaattgc accccaggga
     1381 tagccaaaac catgtgtaaa aagggtaatt ctgtgagtgg cagcttacca ggttaatcaa
     1441 aaaaatcagg taaaatttga ttttttcaaa atcaaatggt aaagttgttt accagaacag
     1501 cggctatgag cagcttttca cggtgccctc gctcggcgaa gcctgcaact tcgtcgtacc
     1561 tgagtagagg tgtctgaccg tgcaagattc taccagtaag ct
//
LOCUS       DAOOQX010000514         1600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_100515_length_1600_cov_3.001294, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000514 DAOOQX010000000
VERSION     DAOOQX010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100515_length_1600_cov_3.001294"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..>1600)
                     /locus_tag="PLX53_07980"
     CDS             complement(3..>1600)
                     /locus_tag="PLX53_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HOY73055.1"
                     /translation="ANNIQFPSGALSELYSVPTQGGRIAQILTTPAEQAVYNPEGTEI
                     AYTDIKGYEDKWRKHHKSSVTRDIWIYDISAGKHRKVTTFEGEDRNPAFAPDGKSLFY
                     LSEMQGNSNVFSVSLSSIGQPESVSSFTNHPVRFLSVARNGMLCYQYNGELYTQKPGS
                     SPQKVNIQIATDQTETRVKYENLSTGITEMNVSPDGKEVAFIIRGDIFVTATDYPTTK
                     RITNTPDQERSVSFSADGRKLLYSSERDGSWKIYESSISSAEEPNFSLSTIIKEQVIV
                     DIPEEAFQPLYSPDGKEVAFLKERTTLCVVNLETKKIRTVLDGKYNYSYSDGDQWFQW
                     APDGNWLAVNFMETPSWPDSDIALVKADGSGEFHNITQSGYDDRNPKWTMDGKAIIWL
                     NDKMGMRSHGSWGSQFDVYSVFLTREAYDEFKLSKQEYELLKEKRSKDKKDNGAGEDK
                     ESSSDKKKEVKAKKDKEAQRKPLIIDYENLDDRKVRLTINSSDLSDAILTDDGEKLYY
                     LSKLRRDTTFGLTTLRIMKPSCW"
BASE COUNT          346 a          363 c          380 g          511 t
ORIGIN      
        1 tttcaccagc agcttggttt cattatcctt aaggttgtta acccaaaggt cgtatccctt
       61 ctcaacttgc tgaggtagta gagtttttcc ccatcatcgg tgagaattgc atcggagagg
      121 tcggatgaat ttattgtgag ccgaactttg cggtcatcga ggttttcata gtctatgatt
      181 aggggtttgc gttgtgcttc cttgtctttt ttagctttta cctccttttt cttatcggat
      241 gagctttctt tatcctcgcc tgctccattg tcttttttat ccttacttcg tttttcttta
      301 agcaattcgt actcctgctt gctcagttta aactcatcat aagcctcccg ggtaagaaaa
      361 acggaataca cgtcgaattg tgagccccat gagccgtgac ttctcatccc cattttgtcg
      421 tttaaccaga tgatagcctt tccgtccatg gtccacttgg ggtttcggtc gtcgtacccg
      481 ctttgggtga tgttgtgaaa ttctcctgaa ccatctgcct ttacaagggc aatatcggaa
      541 tcgggccacg aaggcgtttc catgaagttt acagctagcc aattcccgtc gggtgcccat
      601 tggaaccact gatccccatc gctataggaa tagttatact ttccgtcgag gacggttcta
      661 atcttttttg tttcgaggtt taccacgcaa agcgtcgttc tttccttaag aaaagcaacc
      721 tctttcccat ctggcgagta taggggctga aaagcttcct cgggtatgtc aacaataacc
      781 tgttctttga tgatggtgga aagtgagaag tttggttcct cggctgatga aattgacgat
      841 tcgtatatct tccagctacc gtcgcgttct gaggagtata gtagcttccg accatcggcg
      901 ctgaagctga ccgaacgttc ctgatcgggc gtgttggtta tccttttggt ggtagggtag
      961 tcggtagcgg taacaaaaat atcacctctg ataataaaag ccacctcctt accatcgggc
     1021 gaaacgttca tttcggtaat gccggtggat aagttttcat actttacccg ggtttcggtt
     1081 tggtcggtag ctatttgaat gttgaccttt tgcggactgc tacctggctt ttgagtgtat
     1141 agctctccgt tgtactggta gcagagcatt ccattccggg ccaccgaaag gaatcgtacc
     1201 gggtggttgg tgaagcttga aacagattct ggttgcccta tactggataa gctaaccgag
     1261 aaaacgtttg agttgccctg catttctgag aggtagaaaa gcgatttgcc atcgggagca
     1321 aatgccgggt ttctgtcttc gccctcaaaa gtggttacct tacgatgctt tccggcgcta
     1381 atatcataaa tccatatatc acgggtaact gacgatttat ggtgttttcg ccatttatcc
     1441 tcataacctt ttatatcggt gtaggcaatt tcagtcccct ctgggttgta aacggcctgc
     1501 tcggctggag tagtgagaat ttgtgctatc cgacctccct gagttggaac tgagtaaagc
     1561 tccgataggg ctcccgaagg gaattgaatg ttgttagcgt
//
LOCUS       DAOOQX010000515         1598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_100754_length_1598_cov_2.293584, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000515 DAOOQX010000000
VERSION     DAOOQX010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100754_length_1598_cov_2.293584"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /gene="guaA"
                     /locus_tag="PLX53_07985"
     CDS             complement(<1..708)
                     /gene="guaA"
                     /locus_tag="PLX53_07985"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOY73056.1"
                     /translation="MYEKIVILDFGSQYTQLIARRIRELNVYCEIYPYNQIPVLDGTI
                     KGVILSGSPFSVNDNASPKPDLSGIRGKLPILGICYGAQLLAASSGGQVMPSKSREYG
                     RATLEVMDVSSSLFKDFPIQSQVWMSHGDTITALPRNFLITASTTDIQVGAFQVTNEQ
                     TYGIQFHPEVYHTTEGITLLRNFVVDICGCSQTWTPASFIEETTRNLRETLGSDKVVL
                     GLSGGVDSSVAALLLHRA"
     gene            complement(811..>1598)
                     /locus_tag="PLX53_07990"
     CDS             complement(811..>1598)
                     /locus_tag="PLX53_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOY73057.1"
                     /translation="FHIRMNRINHIFITHLHGDHVLGLFGLISTFNLLGRKQPLHLFA
                     PKELESVLNFNVGVFTDKLSFPVIFHPVDPNMSQLVFEDRHVSVTSLPLRHKVPTVGY
                     LFKETPSLPNIRKDCIQQYSLGLADIVKIKNGADYIAPDGKIIPNAELTFHARLPRSF
                     AYCSDTLYSERLVGLIRGVDLLYHEATFLDADVDLARQTGHSTALQAATAAVGAHVGK
                     LVIGHFSSRYKNISQHLQEARNIFPETYAAEDGQVYAIPEKFG"
BASE COUNT          446 a          353 c          392 g          407 t
ORIGIN      
        1 tgccctgtgc aaaagcaggg cagcaactga agagtcaacc ccacccgata gtcctaatac
       61 taccttatcg ctgcccagag tttcccgaag atttctggta gtttcctcaa taaacgatgc
      121 aggtgtccat gtttggctgc aaccgcaaat gtccaccaca aaatttcgga gtagggtaat
      181 tccctctgta gtatggtaaa cctcaggatg gaactgaata ccataggttt gctcgtttgt
      241 tacctgaaat gcgccaacct gaatatccgt ggtgctggca gtaatcaaaa agttacgggg
      301 gagcgcggta atagtatcgc catgcgacat ccaaacctgc gactgtatgg ggaaatcttt
      361 gaataagcta gatgaaacat ccattacttc aagtgtagca cgtccatact ccctactttt
      421 agaaggcatc acctggcccc cgctgctggc ggcaagtagc tgggctccat agcaaattcc
      481 gagaattggc aatttccccc taattccgct caaatcgggt ttaggagagg cgttatcatt
      541 cacggaaaat gggcttcccg aaaggattac tcctttaatt gtgccatcca acaccgggat
      601 ttggttgtag gggtatattt cacagtaaac atttaactct cttattcggc gggcgattag
      661 ctgcgtgtac tgtgagccga aatcgagtat aacgattttt tcgtacattg aaaaatgact
      721 atttcagaat ttgcaaaggt ataaaataaa ccttaaatga actaggaata tggtagttgg
      781 ggttaaaatt ctgttgtaac aagcatccgg ttagccgaat ttttcgggaa tggcatatac
      841 ttggccatct tcggcagcat aggtttcagg gaaaatattt cttgcctcct gcaggtgttg
      901 cgaaatgttc ttgtaacggg atgaaaaatg tccaatgacc agcttcccaa cgtgcgcccc
      961 aacggcagct gttgcagctt gcaaggcagt ggagtgtccc gtttgacggg ccagatctac
     1021 atctgcatcc agaaaggtag cctcatggta aagtaaatcc acacctctaa tcagcccaac
     1081 cagtctctcg ctgtagaggg tatcggaaca gtatgcaaat gagcggggca accgggcatg
     1141 gaaggtcaac tccgcatttg gaatgatttt gccatcagga gcgatatagt cagcgccgtt
     1201 tttaattttt acaatatcgg ccaagcccag tgagtattgc tgaatgcagt cctttcgtat
     1261 gttgggtagc gagggggttt ccttaaaaag ataacccacg gtaggaactt tatgcctaag
     1321 gggtagcgag gtaacgctga catgcctatc ctcaaaaacc agctgcgaca tgttggggtc
     1381 aaccggatgg aaaataacag ggaacgagag tttatcggta aaaacaccca cgttgaagtt
     1441 tagcacgctt tccagctcct taggggcaaa cagatgcagg ggctgcttcc gacctaatag
     1501 gttaaaggtt gaaatcaggc cgaaaaggcc cagaacgtga tcgccatgaa ggtgggtgat
     1561 gaaaatatgg ttaatacgat tcatccggat atgaaagc
//
LOCUS       DAOOQX010000516         1593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_101261_length_1593_cov_2.783485, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000516 DAOOQX010000000
VERSION     DAOOQX010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101261_length_1593_cov_2.783485"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLX53_07995"
     CDS             <1..416
                     /locus_tag="PLX53_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73058.1"
                     /translation="FNSYRQATESFIASSNASLNEYITTIRPMMETIQNSVDNLLLIN
                     QQALTETAAILKNSPYRTIMPGLIVIITSVVFSMLFYYMISYYFVKPVIGITKGINDF
                     IKYKRPFEVAVDTKDEVNELKEAVKNLTTLKNTPR"
     gene            420..>1593
                     /locus_tag="PLX53_08000"
     CDS             420..>1593
                     /locus_tag="PLX53_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOY73059.1"
                     /translation="MRLHVLSRYIGFVLLLNAAFILFSLLLSIHYHDSGFYPLLFTLL
                     LTTLWAVFPMIFVPKTQSLSNKEGYLVVVLSWIISCFIGMLPLLLYGGEFTVVKAFFE
                     SVSGYTTTGASVLKDVEALPKSLLFWRSSMHWIGGIGIVVFMLVILPSLGKAQLTLSR
                     MEISPLAQENFRFRTRKMLNVILYVYIGLTLCNIILLRIFGMNLFDAVTHAFATIATG
                     GFSTKNASIAWFNSVPIEMVTMFFMFVSGIHFGLLYSTFTFKKSNVFRSPIVKYYFWV
                     TIAGIVAVAINLHGSTYDTWGECFRFASFQVISLSTTTGFANADSSIWPPLSIVIIIF
                     FTIQCACAGSTAGGLKVDRVVIFFKALKSQVLKIEHPQAVIPVRVGNRNISDEVVSS"
BASE COUNT          414 a          387 c          318 g          474 t
ORIGIN      
        1 tctttaacag ctacaggcag gctacagaat catttattgc atcgtctaac gcctcactga
       61 acgaatacat tacaactata agaccaatga tggaaaccat acagaacagt gtggacaacc
      121 tcctgctaat aaaccagcaa gccctaaccg aaacagctgc cattcttaaa aatagccctt
      181 accggacaat catgcctgga ttgatagtga ttatcaccag cgtagtgttc tccatgctat
      241 tttactacat gatttcctac tactttgtta agccagtaat cgggataacg aaggggatta
      301 atgatttcat aaagtataaa cgtccatttg aggttgctgt tgataccaag gatgaggtaa
      361 acgagctgaa ggaagctgtc aagaatctta ccaccctaaa aaatacaccc aggtaataga
      421 tgagacttca tgttctttcc cgctacattg gatttgttct cctgctaaac gcagctttca
      481 tcctgttttc acttctacta tccatccatt accacgatag tggtttttac ccgctactct
      541 tcaccctgct gctaaccacc ctatgggcgg ttttccccat gatatttgtt ccaaagacac
      601 agagcctttc caacaaggaa ggctacctgg ttgttgtgct cagctggatt atatcatgtt
      661 tcattggaat gcttccgctt ctactttacg gtggtgaatt tacggtggtg aaggcattct
      721 tcgagagcgt ttcgggctac accacaaccg gagcttcagt tttaaaagat gtggaagccc
      781 tgcccaaaag cctgctattc tggcgctcct ccatgcactg gattggaggt attggcattg
      841 ttgttttcat gctggttatt ctcccctcgc tgggtaaagc ccagctgacc ctatccagaa
      901 tggaaatctc accacttgcc caggaaaact tcagattccg aacacgcaag atgctaaacg
      961 tcatcctcta cgtgtacatc gggctaaccc tttgcaatat catcctgtta agaatttttg
     1021 gcatgaacct gtttgatgcg gtaacccatg cctttgctac cattgccaca ggggggtttt
     1081 caacaaaaaa cgccagcatt gcatggttta acagcgtgcc tattgagatg gtaaccatgt
     1141 tcttcatgtt tgtttcgggt attcattttg ggcttctata ctcaactttt acctttaaga
     1201 aaagcaatgt tttccgctcc cccattgtta aatactactt ttgggtaacc atagccggaa
     1261 tagttgcagt tgccatcaac cttcatggat caacctacga tacatggggc gaatgcttta
     1321 ggtttgcctc ttttcaggta atttccctct ccaccaccac gggttttgcc aatgccgatt
     1381 catctatttg gccaccccta tctattgtca tcatcatatt ttttaccatt caatgcgcct
     1441 gcgcaggttc aacagccgga ggtttaaagg ttgacagggt tgttattttc tttaaagcgc
     1501 tcaaaagtca ggtgcttaaa attgaacacc cccaggcggt tatcccagtt agggttggca
     1561 acagaaatat ttccgacgag gtggtaagct cgg
//
LOCUS       DAOOQX010000517         1590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_101561_length_1590_cov_3.866450, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000517 DAOOQX010000000
VERSION     DAOOQX010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101561_length_1590_cov_3.866450"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1074)
                     /locus_tag="PLX53_08005"
     CDS             complement(13..1074)
                     /locus_tag="PLX53_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY73060.1"
                     /translation="MNADRLIKSPQAVALIISVISALIAGVVSAAIFGVATEKQVLSC
                     VAIAMASMAGTFIASTILVSLFAARKIKPLYKILYQVDVKNSDIANEFEDNNLLENIN
                     DSLVSWTTSKTEEIDRLKEMERYRKEFLGNVSHELKTPIFNIQGYVLTLLDGGLADSH
                     INRKYLERTEKSINRMIGIVEDLEIISKLEAGELKLNMERFNIVGMVEDIFEALEERA
                     RKKSISLEFDKVYERPVWVYADKKRIYQVLHNLIINSINYGIQHGHTVVAFSDSGKRV
                     TTWVTDDGIGISQKDIPRIFERFYRVDKSRSREQGGTGLGLAIVKHIIEGHKQSIKVE
                     SSLNKGTTFSFTLDKPTKQ"
     gene            complement(1140..>1590)
                     /locus_tag="PLX53_08010"
     CDS             complement(1140..>1590)
                     /locus_tag="PLX53_08010"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOY73061.1"
                     /translation="TVTAIRETLEKSNLGTLKVGDKVNVERSMRIDSLLDGHLVQGHV
                     DQTAVCTGVEEAEGSWYYTFEYDPSKGNITVEKGSIAVNGVSLTVVNSRSSSFQVAII
                     PYTYEHTNFHQIQKGTVVNLEFDIVGKYITKMLRQYIESGQIANLSR"
BASE COUNT          363 a          434 c          340 g          453 t
ORIGIN      
        1 tttgtttcca catcactgct tggttggctt gtcaagggtg aagctaaatg ttgtaccctt
       61 gttaaggcta ctctccacct tgatgctctg cttatgaccc tcgatgatat gcttcacaat
      121 ggcaagtcca agccctgtgc ctccctgttc gcgcgaccgg cttttatcaa ccctgtagaa
      181 acgctcaaaa attctcggaa tgtccttctg gctaatgcct attccatcgt cggttaccca
      241 ggttgttacc cttttgcccg aatcggaaaa cgcaacaaca gtgtggccat gctgaattcc
      301 gtagtttatt gagttgatga tgaggttatg cagcacctgg taaattcgct ttttatcggc
      361 gtaaacccac accggccttt cgtaaacctt gtcgaactca aggcttattg atttcttcct
      421 ggcccgctcc tcaagcgcct caaagatatc ctctaccatg ccgacaatgt tgaaccgttc
      481 catgttcagc tttaactcac cagcctcaag cttcgagata atttccaaat cctccacaat
      541 gccgatcatc cggtttatgc ttttttcggt ccgctccagg tatttcctgt tgatatggga
      601 atcggcgagc ccaccgtcga gcagcgtcag cacgtacccc tgaatgttga aaattggcgt
      661 tttcagctca tgcgacacat ttcccaaaaa ctcctttcgg taccgctcca tctcctttaa
      721 ccggtcaatc tcctcggttt tgcttgttgt ccagcttacc agactatcgt tgatattctc
      781 gagcaggttg ttatcctcaa attcgttggc aatatcggaa ttcttcacat ccacctggta
      841 tagaatttta tataggggtt tgatttttcg ggcggcaaaa aggcttacca gaattgtgga
      901 agctataaat gtgccggcca tcgatgccat ggcgatggcc acacaggata atacttgctt
      961 ttcggtggct accccgaaaa tggcagccga taccacaccg gctattaagg ccgaaataac
     1021 cgaaatgatg agggctactg cctggggaga tttaatcaga cgatcagcat tcatttgaat
     1081 gggttattcg ttacccaaac cacagctctg ttgtaaaacc tgacagctcc cttcatggcc
     1141 tacctggata agttggcaat ttgacccgat tcgatgtact ggcgaagcat cttggttatg
     1201 tacttaccca cgatgtcgaa ctccagattc accacagtcc ccttttgtat ctggtgaaag
     1261 ttggtgtgct cgtaagtgta gggaataatg gccacctgaa acgagctgct gcgcgagttc
     1321 accacggtga ggctaacccc gttaacggca atggagccct tttcaaccgt tatattcccc
     1381 tttgaagggt cgtactcaaa ggtgtagtac cagctgccct cggcttcctc cacacccgtg
     1441 cacacagcgg tttggtcaac atgcccctga accagatgcc catcaagcag gctgtctatc
     1501 ctcatgctac gctctacgtt caccttatca cccaccttta gcgtccccag gttcgatttc
     1561 tccagggttt cccttatggc cgtaacggta
//
LOCUS       DAOOQX010000518         1589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_101717_length_1589_cov_2.237940, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000518 DAOOQX010000000
VERSION     DAOOQX010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101717_length_1589_cov_2.237940"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1589
                     /locus_tag="PLX53_08015"
     CDS             <1..>1589
                     /locus_tag="PLX53_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOY73062.1"
                     /translation="AELREAIVDYHVGSVLNSSNNRARSLAAWNSIVSTIQNVAIAKT
                     RLAIPVLYGIDAVHGANYTAEATLFPQQIGMAATWNDSLVQAIGSITAYETRASGIPW
                     VFSPVLDLGADPRFPRQYEGFGEDPHLCSVLGVALVNGYQGASVSSPLKVAACAKHYL
                     GYSAPSSGKDRTPAQLPNITLKEYHLPPFKAAVEAGVKTFMVNSGVINGLPVHASHYI
                     ITELLKEELGFEGVVVSDWNDIENLYRRDRVVATEKEAIGLAVNAGIDMAMIPYRYQD
                     FCNQLVELVREGSVEESRIDDAVMRILKLKAELGLFERPTTVLGDYPKAGSEEFAKVS
                     YQAAGESITLLKNSNGILPLSKDKRYKILVAGPNANSMRTLDGGWSYSWQGEKVEEFA
                     GRYNTIFEAIKSKVPQSEVSYIPGVSYPAYGRYFEEQYDKFEESAAAARKADVIILCL
                     GENSYAEKPGDLNDLYLGSKQLNLAEELAKTGKPIILVLNEGRPRVISPIEPMMAAVV
                     QTYLPGSYGADALADILFGDI"
BASE COUNT          410 a          366 c          425 g          388 t
ORIGIN      
        1 ggctgaacta cgggaagcaa ttgttgacta ccatgtgggt tcagtgctaa acagcagcaa
       61 taatcgagct cgctcactgg ctgcgtggaa cagcatagtt tctaccattc agaatgtagc
      121 catagctaaa actcggctag ctataccagt tttatatgga attgatgcgg ttcatggcgc
      181 taactatacg gctgaggcca ctctttttcc acagcagata ggcatggcgg caacatggaa
      241 cgattccctt gttcaggcaa ttggaagcat tacagcctac gaaacacgtg caagcggaat
      301 accatgggtt ttctccccgg ttcttgattt aggagccgac cctcgtttcc cccgacagta
      361 tgagggattt ggcgaggatc cgcacttgtg cagtgttttg ggggttgcgc tagtaaatgg
      421 ctaccaggga gcaagtgtat caagtccgct gaaagttgct gcctgtgcca agcactacct
      481 gggctactcg gcgccctcat ccggcaagga tagaactccg gctcagctgc caaacattac
      541 gctaaaggaa taccacctgc ctccatttaa agctgctgtt gaagcggggg ttaaaacgtt
      601 tatggttaac tccggggtaa ttaacggact tccggttcat gccagccact acattattac
      661 cgagctactg aaggaggaac ttggttttga aggggtagtg gtttctgatt ggaacgacat
      721 tgaaaacctt taccgtcgcg atagggtcgt ggccactgaa aaagaggcta tagggctggc
      781 tgtaaatgcc ggaattgata tggctatgat tccttaccgt taccaggatt tttgtaacca
      841 gctggtggag ctggtgaggg aggggtcggt agaggaaagc cgaattgacg atgctgtgat
      901 gcgaatactt aagcttaagg ctgaacttgg attgtttgag cgtccaacta cagtgctggg
      961 cgattacccc aaggctggct ccgaggagtt tgccaaagtt tcataccagg cggcaggcga
     1021 gtctattacc ctgctcaaaa atagcaacgg catcctgccc ttaagcaagg ataaaaggta
     1081 taaaattctt gttgccggcc ccaacgcaaa ctccatgaga accctcgatg gtggatggtc
     1141 atactcgtgg cagggtgaga aggtagaaga atttgccggc aggtacaaca ccatttttga
     1201 ggccattaaa agtaaagtgc cccaatcgga ggtcagctat attcccgggg taagctaccc
     1261 ggcttacgga cgatactttg aggaacagta cgataagttc gaagagtctg ctgctgccgc
     1321 tcgtaaggcc gatgttataa ttctttgcct tggcgaaaac agctatgctg aaaaacccgg
     1381 cgaccttaac gacctgtact tggggagcaa gcagcttaat ttggcagaag agttggccaa
     1441 aaccggtaag cccatcatac ttgttttaaa cgaaggacga cccagggtta ttagccctat
     1501 tgaacccatg atggcagcgg tagttcaaac ctaccttccg ggtagctatg gtgccgacgc
     1561 actggccgac atcctttttg gcgacatta
//
LOCUS       DAOOQX010000519         1584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_102172_length_1584_cov_3.100719, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000519 DAOOQX010000000
VERSION     DAOOQX010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102172_length_1584_cov_3.100719"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1070)
                     /gene="rlmN"
                     /locus_tag="PLX53_08020"
     CDS             complement(39..1070)
                     /gene="rlmN"
                     /locus_tag="PLX53_08020"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOY73063.1"
                     /translation="MEKQNNKVPLIGFTPQELCSTMQEYGGTSFQAESLMRWIYRKRE
                     KDISTFSDIPKAILQSVEQSYTVGLYPPKESKRSDDGSVKYLFANGQNQVFEAVAMSS
                     GNRHTLCLSTQSGCRMGCRFCRTGSIRFNGNLSAGDIINQVLSIPDSKRLNRIVLMGM
                     GEPLDNLKVVNKTLEILTADWGCALGKSNITISTVGLLSELEAFLKSPGCNLAISLHS
                     PFPDERAMLMPVERTNPILEIIKLLKHYPLKKPLRLTFEYVALGSLNLSKNHARAIAT
                     LLSGLSCHLNIIPWNRHNAATFQSPTSDELTNFMELLNDAGIPTNLRKSMGGDIDAAC
                     GQMAGNAPH"
BASE COUNT          418 a          336 c          366 g          464 t
ORIGIN      
        1 gttaggatta ttaaattttg attgatccag ctttagtctc aatggggggc attgcccgcc
       61 atttggccac atgccgcatc aatatcccct cccattgatt tgcgcaggtt tgtcgggata
      121 cctgcatcgt tgaggagctc cataaagtta gtaagttcgt cgctggtggg tgattggaag
      181 gttgctgcat tgtgccggtt ccatgggata atgttgagat ggcagcttag tccacttagc
      241 aatgttgcta ttgccctggc gtgattttta gatagattta ggctgcctaa cgcgacatac
      301 tcaaaggtga gccttagcgg tttttttagg gggtagtgtt tgagcagctt gattatttca
      361 agtattggat ttgttctttc aacgggcata agcattgccc tttcatcggg aaaaggtgaa
      421 tgcaggctaa ttgccaggtt acagccgggc gattttagaa aggcctccag ttcggaaagc
      481 aaaccaactg ttgaaatggt aatattcgat ttacctaagg cgcaacccca atcggctgtc
      541 aggatttcaa gcgttttgtt tacaactttc aaattatcaa gcggttcgcc catacccatc
      601 agcacaattc tgttcaacct tttgctatct ggtatgctaa gcacctggtt gatgatgtcg
      661 cctgctgata ggttgccatt aaagcggatg cttcctgtgc gacaaaatct gcatcccatt
      721 ctacaccccg attgggttga caggcatagg gtatgccgat tgcctgaact catggctaca
      781 gcttcgaaaa cctggttttg cccgttggca aacaggtact tcacagagcc atcgtcgctc
      841 cgtttacttt cttttggagg gtatagacct accgtatagg actgttcaac tgattgtaga
      901 atagcttttg gtatatcgga gaaggtagaa atatcctttt cgcgtttacg gtatatccag
      961 cgcatgaggc tttcagcctg aaagctagtt ccaccatatt cctgcatggt gctgcaaagc
     1021 tcctgtggag tgaaacctat gagcggaacc ttgttatttt gtttttccat caacttcaat
     1081 tagcacaggt ttactgaaag gcatatcaac atatcaacca atgctgtaag cgctattctg
     1141 attattgggc ctccatgtaa aattgaatac ttggcaattc tttttacctt tgtatccacg
     1201 aaagcatcaa tccaaatgag caatcggaca ggagcacatt ctgcatatca aaagagcatt
     1261 cttttcattc ttgcttttgt gatattgctc cccctattcg ggtgtagcaa cagaacggtt
     1321 gttcaaaaat cgggaaaggc taagtacacc aaaaagtata agtgcaaatg tagtaagcct
     1381 aaggcgaacc gtcaaaccta caagtatctg aaatgagtac aaatgctgag attgagaaaa
     1441 aaccttctat tgccgtagtt ggaagcggaa gttgggcaac cgcactggca aaggtgctac
     1501 atgaaacaca ggactcaatt ggctggtgcc ttagggatcc ggagttaatt ggctatatta
     1561 aaaagtacag gcataacccc aact
//
LOCUS       DAOOQX010000520         1581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_102477_length_1581_cov_2.848624, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000520 DAOOQX010000000
VERSION     DAOOQX010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102477_length_1581_cov_2.848624"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1525)
                     /locus_tag="PLX53_08025"
     CDS             complement(<1..1525)
                     /locus_tag="PLX53_08025"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOY73064.1"
                     /translation="MQHLHELYKFYLKHPSLKQLLDAGQNLEGGFFLKGLAGSAVAFV
                     LSGYVEQRRRPSLVVVNDKEEAAYLFSDLSCLLPENQVYFFPSSYKRSVQYGQTLPDA
                     IVQRTDVVALLPALNENDERPVVVVTYPDALFEKVVSLDTLSRNSIKIQKGEKVSPDF
                     ICDTLTEYGFQRSDFVYEPGQFSVRGSIVDVFSYSASTPYRLDFFGDEVETIRSFDVE
                     DQLSKELLSEVVIIPNLQSPEDNPSRILLTDLLPPSTTLWVKNPELVLNRMDELSRIV
                     STATQLNASNFASRDIFSNSLLTRSTLYFGIKPLSVQLPTFDFGTSPQPAFGKNFELL
                     VQKINEDAGRGYQTFILSDNSAQLERLDGIMHGIAPDIAYNPVEGTISEGFVDHNLRI
                     NLYTDHQIFERYHRYKLKHEFNRRDAITMQELVDLQPGDYVVHIDHGIGIFGGLVSTE
                     INGKKQEAIRLVYQDNDTLLVSIHNLHKISKYRGKDAESPKIYKLGSGAWQRLKQNTK
                     "
BASE COUNT          422 a          376 c          371 g          412 t
ORIGIN      
        1 ttttggtgtt ctgcttaagc cgctgccatg cacccgaacc cagcttatag attttaggag
       61 attcggcatc ctttccacgg tactttgaaa tcttatgcag gttatgaatg cttaccagca
      121 gggtatcgtt atcctgatac accagcctga tagcctcctg cttttttccg ttaatctcag
      181 ttgaaacaag acctccgaaa attccaattc catggtctat atgcaccacg tagtctcccg
      241 gctgtagatc taccaactcc tgcatggtta tggcatcccg acggttaaac tcgtgtttca
      301 gtttgtaccg gtggtacctt tcaaatatct ggtggtcggt gtacaggtta attcggaggt
      361 tatggtcaac aaaaccttcc gagatggtcc cttccaccgg gttgtaggca atgtcggggg
      421 caataccgtg cattattcca tcgagccgct ccagctgagc gctgttgtcg gacagaataa
      481 aagtttgata gcccctcccg gcatcctcgt tgattttttg aacaagaagt tcaaaatttt
      541 tcccaaatgc gggttgggga gatgtgccaa aatcgaaggt gggtagctgc acggacaacg
      601 gtttaatacc aaagtatagg gttgaccggg tgagcaggct gttggaaaaa atgtctcggg
      661 aagcaaagtt actggcgtta agctgggttg ctgtggaaac aatacggctt agctcatcca
      721 tccggttcag caccaattcc ggatttttta cccagagcgt ggtgcttgga ggcagcaaat
      781 cggtcagcag aatccttgaa gggttgtcct cgggcgattg caggttggga ataataacta
      841 cttcgctgag cagctccttc gatagctggt cttccacgtc aaacgaccta atagtttcca
      901 cctcatcacc aaagaaatca agcctatagg gagtggaagc agagtatgag aaaacatcca
      961 caatgctccc ccttacggag aactgtccag gttcataaac aaaatcggaa cgctgaaagc
     1021 cgtactctgt aagtgtatcg caaatgaagt cgggagaaac tttttcgccc ttctggattt
     1081 ttatgctgtt acggctgagc gtatccaggc tcaccacctt ttcaaatagg gcatcgggat
     1141 aggtaacaac tacaacaggc ctttcgtcat tttcgttgag agctggaagg agcgctacaa
     1201 cgtcagtccg ctgaacaata gcatcgggga gagtttgccc atactgaacc gaacgcttgt
     1261 aggacgatgg gaaaaagtac acttgatttt cgggcagcag acacgacaaa tcactaaaaa
     1321 gataagctgc ttcctcctta tcgtttacca caaccagcga tggccgtcgc ctctgctcaa
     1381 cgtaaccgga cagtacaaat gcaaccgcag acccggcaag gcctttaagg aaaaaaccgc
     1441 cctccagatt ctgaccagca tccaaaagct gcttcagcga aggatgcttt aggtaaaact
     1501 tatatagctc gtgtaggtgt tgcaataaaa aaattttcag caaaagtaag aatctgctcc
     1561 aagattcctt tttctttctt t
//
LOCUS       DAOOQX010000521         1576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_102955_length_1576_cov_3.820513, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000521 DAOOQX010000000
VERSION     DAOOQX010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102955_length_1576_cov_3.820513"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..868
                     /locus_tag="PLX53_08030"
     CDS             236..868
                     /locus_tag="PLX53_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73065.1"
                     /translation="MRKIIANLTKHSIKILSYLLVATGVMLIGSARAQDQEQGRNFDR
                     FSDRLFFGGSFGLAVGSRVTQVDVAPMMGIWIFPEWSLGLGGRYSYRRERFSINGFTT
                     EPIETHVWGVSGFTQVIPITDFHRTFGIDLHGGIILHGEFESLYLDRNFFDQSSSYGK
                     GWVNMYLVGGGWHQPIGDRAAINFLVLWDLTDNRYSPYNSNPILRFTITF"
     gene            complement(921..1121)
                     /locus_tag="PLX53_08035"
     CDS             complement(921..1121)
                     /locus_tag="PLX53_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOY73066.1"
                     /translation="MKGKVKWFDAAKGFGFIQTEEGSDVFVHYTGIAKDGFKRLDEGQ
                     EVSFEITEGKRGPQATNVVVLE"
     gene            1363..>1576
                     /locus_tag="PLX53_08040"
     CDS             1363..>1576
                     /locus_tag="PLX53_08040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOY73067.1"
                     /translation="MNISNDKVVSISYELKVNGDLVDSAVADNPLVFLYGHGQLLPLF
                     ETNIHNLKTGDSFEFSIPYADGYGEVD"
BASE COUNT          433 a          305 c          321 g          517 t
ORIGIN      
        1 gatttcgcaa gctgcagtac tgtttggtat ccaatacctc tggaggctcc ggttacaata
       61 atattcataa ttttttgttt actaaccttt tttctttcat tttgttttga taaaattgca
      121 cgagcaggca gatttccgtt ccgtatatac ggcattacct tgaccctatc ctgggtggct
      181 aattaaaaaa gggaaggtaa tactttattt cataaacctg attagtgttg tccgtatgag
      241 aaaaataatt gccaacttaa ccaaacattc aattaaaatt ctttcctatc tcctcgtggc
      301 cacaggagtt atgttgatag gttcagcaag agcgcaggac caggaacagg gtagaaattt
      361 tgaccgattt tccgataggc tattttttgg gggaagtttt ggattggctg ttggtagcag
      421 ggttacgcag gttgatgttg cccccatgat gggtatatgg attttcccgg agtggtcttt
      481 agggttaggc gggcgctaca gctaccgtcg tgaacgcttt agcattaatg ggttcaccac
      541 cgaacctata gagacacacg tctggggagt gtcgggattt acccaggtta ttcctattac
      601 cgatttccat aggacttttg gcattgatct gcatggggga attattttgc atggtgaatt
      661 tgaaagtctt tacctcgacc gaaacttttt tgaccagagt agcagctatg ggaaaggctg
      721 ggtgaatatg tacctggttg gaggaggttg gcaccaaccc attggcgata gggctgccat
      781 taacttttta gtgctgtggg atttgaccga caaccggtat tcaccctaca acagcaatcc
      841 cattttgcga tttaccatta ccttctaata aaaaaaggcc tccattggag gccttactgg
      901 ctagtatgtt aggaataaac ttattctaac actaccacat tggtagcctg tgggccacgt
      961 tttccttcgg taatttcaaa acttacctcc tgaccttcgt caagacgctt gaaaccatcc
     1021 ttagcaattc ctgtgtagtg aacaaataca tcactacctt cctcggtttg aatgaagcca
     1081 aagccttttg ctgcatcaaa ccattttact ttacctttca tgttaaaatt gatataaatg
     1141 atgaattaaa ttaattaata gcgttgcaaa tatggcatat aaaattgaat attcaacatt
     1201 attctaaaaa aaaaattatt tattgttaaa atctttgcct caagggatat acatgctttt
     1261 aagtaaggtc gctttttcat tcgaacattt ggggatttct attgtttttt accgaaatta
     1321 gatattcctt tggcaattat taattaaatc aaatcaattt acatgaacat ttctaatgac
     1381 aaagttgtat ccattagcta tgagttaaaa gtcaatggcg atttagttga ttcggcggtt
     1441 gctgacaacc cgttagtttt cctttacggc catggccagc tgctgcctct cttcgagacc
     1501 aacattcaca acctgaaaac aggtgattca ttcgaatttt cgattcccta tgctgatggt
     1561 tatggagaag ttgacg
//
LOCUS       DAOOQX010000522         1576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_102984_length_1576_cov_2.942801, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000522 DAOOQX010000000
VERSION     DAOOQX010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102984_length_1576_cov_2.942801"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="PLX53_08045"
     CDS             complement(<1..1090)
                     /locus_tag="PLX53_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOY73068.1"
                     /translation="MEKPDNTDSSGIEDKYQALLGLIPDMIFIFDSDVRYVGYHGGKD
                     APTYVPPEMFLGKRIEEVLPEPVASNARVVVEEVLKTGKMQTYSYQLTIDNKPQFFES
                     RMVRYGTSKVLAIVRDVTSLTNAENALKESEEKYYNLYNLMRLMADTIPDMVWAKDLN
                     QNYIFANKAFCSTILNARDTDEPVGKNDMFFAKRERESHPENPEWHTFGELCMNSDLD
                     TLRELKEMQFDEYGNVKGKFIFLDVHKSPLYDKNGKLIGVVGSGRDITEKKKMEEHLA
                     LSEKTYRGIINSISEAVYVQDADGTFLDVNKACEKYYGYPREYFIGKKPKDLSAPEIN
                     DLDKVTDYVSKAFDGQPQIFEFWGLRNDG"
BASE COUNT          415 a          358 c          336 g          467 t
ORIGIN      
        1 tgccatcatt tcggagaccc caaaattcga agatttgggg ttgaccgtcg aaggccttac
       61 taacgtaatc ggtaacttta tccagatcat ttatctccgg agccgataaa tcctttggct
      121 ttttccctat aaaatactcc cgcgggtatc catagtattt ttcgcaggcc ttatttacat
      181 ccaggaaggt gccatcggca tcctgaacat aaaccgcttc ggatatgctg ttgataatgc
      241 cgcggtaggt tttttcagat agagccaggt gttcctccat tttctttttc tcggttatat
      301 cccttccgct tcctactacg cctattagtt ttccattctt atcgtacagg ggtgacttat
      361 gcacatcaag aaagatgaac tttcccttaa catttccata ctcatcaaac tgcatctcct
      421 tcagctcacg aagggtatcc aggtcagagt tcatacataa ttcaccgaat gtgtgccact
      481 caggattttc agggtgggat tccctttccc ttttggcaaa aaacatgtca tttttgccaa
      541 ctggttcatc ggtatccctg gcgtttagta tggtgctgca gaatgccttg ttggcaaaaa
      601 tgtagttttg atttaagtcc tttgcccaca ccatgtcggg aatagtgtcg gccattaacc
      661 tcatcaggtt gtaaaggttg tagtactttt cctcgctctc cttaagggca ttctccgcat
      721 ttgtaagact ggttacatcc cggacaattg ccaaaacctt ggaagtgccg tatctaacca
      781 tccggctttc aaagaattgt ggcttgttat caatggtaag ctggtagctg taggtttgca
      841 ttttaccggt tttcagcacc tcctcaacca caactcgggc atttgaagca acaggctcgg
      901 gcagcacttc ctctatgcgt ttgccaagaa acatttcggg tggaacatag gtaggtgcat
      961 ccttgccgcc atggtagccg acataccgta catcggagtc aaaaataaaa atcatatcgg
     1021 ggattaagcc aagcagcgcc tggtatttat cctcaatacc gctactgtca gtattgtctg
     1081 gtttttccat tttgagcaaa gtaatgttat taatttttac aatttaggta aacgatagga
     1141 gaaagtcaac attttttaag tgaacatgat gtaatagggg ataaagtgaa aaagataaag
     1201 gataaaaatg ttcaaggtta agaacttttt tattaaaaga gttatttagc cccgaagggg
     1261 cgagaaagcc tagcccaggg caaagccttg ggtttaactc caaagcataa ttttgaccct
     1321 ttgcatcgca acgggaaaaa caactttaaa tattccgccc cttcagggct taatatatca
     1381 taccgccacg ccacaagggg ctgcgcccct tgctacctat tccgccccgt tggggctaaa
     1441 gaaagaggaa agtataaagt ggatttgtcc ggttttagaa ggattcaccc ttcgacaaac
     1501 tcagggcagg ctccttcgac tggtgttaca gtgagtagtt cggctggtgt tatagtgagc
     1561 cagtcgaact actcac
//
LOCUS       DAOOQX010000523         1575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_103104_length_1575_cov_3.092105, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000523 DAOOQX010000000
VERSION     DAOOQX010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103104_length_1575_cov_3.092105"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1192
                     /locus_tag="PLX53_08050"
     CDS             134..1192
                     /locus_tag="PLX53_08050"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HOY73069.1"
                     /translation="MSYNRIVELLGDKSEFLLNHKCETVKKEQLHLPGPDFIERVVAL
                     SNRSPQVLRNLQEMFNHGRLAGTGYLSILPVDQGIEHSAGASFAPNPIYFDPENIVRL
                     AIEGGANAVASTFGVLASVSRKYAHRIPFIVKLNHNELLTYPNKYDQIMFGSVEEAWY
                     LGAVAVGATIYFGSEESTRQIIEVAQAFERAHELGMVTVLWCYLRNNAFKKDGVDYHV
                     AADLTGQANHLGVTLQADIIKQKAPENNGGFKAIGFGKTSDKVYSQLSSDHPIDLTRY
                     QVVNCYNGRAGLINSGGASSGSSDLAEAVFTAVVNKRAGGMGLISGRKAFQRPMDEGV
                     TILNAIQDVYLESKIDIA"
BASE COUNT          411 a          331 c          356 g          477 t
ORIGIN      
        1 catgggttcc cctgcagcaa aaatttatgt ggagggatat taccttcaac tttggtattg
       61 gctatccttt ctaagatttt gttttatacc ctatttttta tagttttgta taaaccatta
      121 taatctcaat gccatgagtt acaatagaat tgttgagctg ctcggcgata agtccgaatt
      181 tcttctaaac cacaagtgtg aaacggtgaa gaaggaacag ctgcacttgc ccggtcccga
      241 ttttattgaa agggttgttg cactatctaa ccggtcacct caggttttac ggaaccttca
      301 ggaaatgttc aaccatggaa gacttgctgg cacaggttac ctgtccattt tgccggttga
      361 ccagggtatt gaacatagcg ctggtgcatc ttttgcccca aatccaatat attttgatcc
      421 ggaaaatatt gtaaggcttg ctattgaggg tggtgcaaat gcagttgcct ctacttttgg
      481 agttttagca tcggtttccc gaaagtatgc ccaccgcatc cccttcattg ttaagcttaa
      541 ccataacgag ctgcttacct atccaaacaa gtacgatcaa atcatgtttg gctcggtcga
      601 agaggcatgg taccttggtg ctgttgccgt gggggcaaca atctactttg gctcggaaga
      661 aagcacccga cagattattg aagtggctca agcctttgag cgtgctcacg agctaggtat
      721 ggttaccgtc ctttggtgct accttcgcaa caatgcgttt aaaaaggacg gggtggacta
      781 ccatgttgct gccgacctta ccggccaggc aaaccatctg ggagttacac tacaggctga
      841 catcataaag cagaaagcac ctgaaaacaa tggggggttc aaggctattg gatttggaaa
      901 aaccagcgat aaggtttaca gccagctttc atccgatcat cccatcgacc taactcgtta
      961 tcaggttgtt aactgctaca atggacgtgc tggccttatc aactcgggag gtgcttcgag
     1021 cggttcgagc gatctcgcag aggccgtatt tacagctgta gtcaataaac gggccggggg
     1081 tatgggctta atcagcggca gaaaagcttt ccagcgccct atggatgaag gtgttacaat
     1141 actaaatgct attcaggacg tttaccttga aagcaagatt gatatagcgt aaatcaactt
     1201 catattttct gtaaacccac aactttgttg ttgtgggttt tttgctaata cccgagcaaa
     1261 catttgcatt tattctgcta attttataaa attgtttacc agaagcttaa gcttccgatt
     1321 ttgagaatgc atttatccgt gttgatttat tgtaaatatt gttttctggc tgcattactt
     1381 taagctggtt ctggttgggt tgattgctcc tttatacata gccggcaggt tgccatgaat
     1441 aagtttaaat caaaattgaa agactacttt tcatttaccc ggagcgagcg caacggaacg
     1501 ctagtactat catttctgct tgtggttttt atgcttgctc ccgcagcaat caggcatata
     1561 aagtttagca ggaat
//
LOCUS       DAOOQX010000524         1571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_103527_length_1571_cov_3.013852, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000524 DAOOQX010000000
VERSION     DAOOQX010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103527_length_1571_cov_3.013852"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..>1571
                     /locus_tag="PLX53_08055"
     CDS             156..>1571
                     /locus_tag="PLX53_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73070.1"
                     /translation="MNHSYTLADKTFFCEQVLPLLHAELNSDTQKHLSTYRHERVKKT
                     VIYIGITTSSVVAGALKTLKAIEGYLEENSVDAIIVPVGSYGLCSFEPIVEVQLPGKK
                     RVAFRNVTYDRVEFLLDGAFNSFVQPDTALFQHNSDILEPWPDIPVKDDFPFFAKQDR
                     LLLRNIGIINPENLLEYISFGGYRALAYTLRNQTSDSVCRIVELSGLRGRGGGGYPTG
                     LKWRKTLSVAATERIMICNADESDPGAFMDRLLVESDPFRIIEGLTISAYAIGANKAI
                     IYIRNRNKLAVDRLTLAMEQAYDFGLLGYNILDSGYSLDISIFQGPGAYVCGEETALI
                     QSLEGKRGTPSIKPPYPNEVGLNGKPTVVNNVETLASIPDILLNGADWFMKIGTATSK
                     GTKIFSVNGKASLTCVVEISMGRPLSAIVELAGGVKPDCTLKAIHLGGPSGVSVTPDE
                     LYLPVDFDELKVHGIPLGSGGV"
BASE COUNT          423 a          342 c          373 g          433 t
ORIGIN      
        1 acctcacacc ggctaaactt ggtgaagttc ttgccgggtt agccgagtcg gagtagtgcc
       61 atttggatat tcaacatttt catccacaat gggttaagtt gtgatgctat atttgatata
      121 cttggagttt tgaacatagc tgttattgaa aggttatgaa ccacagttac actttagccg
      181 ataaaacttt tttttgtgaa caggtgctac ccttgcttca tgccgagcta aatagtgata
      241 cccaaaaaca cttatcaaca tacaggcacg aaagggtaaa aaaaacagtc atatacattg
      301 gcattacaac ttcatcggtt gtggctggcg ccctgaaaac tctgaaagca atagagggct
      361 atcttgaaga aaattcagta gatgccataa ttgtacccgt tggcagctat ggcctttgct
      421 ccttcgagcc aattgttgag gttcagctgc cggggaaaaa gcgggtggcc ttcagaaacg
      481 taacatacga tagagttgaa ttcctgctcg atggtgcatt taacagcttt gttcaacccg
      541 atacggcact ttttcagcac aattccgata ttcttgaacc gtggcccgat attccagtta
      601 aggatgattt cccttttttt gcaaaacagg ataggctact tcttagaaat attggaatta
      661 taaatcccga aaatctactg gagtacatct cattcggagg ttaccgggca cttgcctata
      721 ccctgcggaa tcaaacatcc gattccgttt gccgtattgt tgagctaagc gggttgaggg
      781 gacgtggagg cggaggctat cctaccggat tgaaatggcg taaaaccctt tcggttgctg
      841 ctaccgaaag gattatgatt tgtaatgctg acgaaagcga tcccggcgct tttatggata
      901 ggctattagt ggaaagcgat cccttccgta tcattgaagg tttaacaata agcgcttatg
      961 ccattggtgc aaacaaagca attatctaca ttagaaaccg taacaagctg gccgttgacc
     1021 ggcttaccct ggccatggag caagcttacg attttggttt gcttgggtac aacattttag
     1081 atagcggcta tagcctcgat atttcaattt ttcaaggccc tggagcttac gtttgcgggg
     1141 aggaaactgc tctaatccaa agcctcgaag ggaaacgtgg tacgccttct attaaacctc
     1201 cctaccctaa cgaggtgggg ttaaatggta agcctaccgt ggttaacaat gtcgaaaccc
     1261 ttgccagtat cccagatatt ctccttaatg gagctgactg gtttatgaaa atagggacgg
     1321 ccacatcaaa aggaactaaa attttctctg taaatggtaa ggcttccctt acctgtgtgg
     1381 tggaaatatc catgggcagg ccactctcag ccattgtaga gctggcagga ggggttaagc
     1441 ccgactgcac gttaaaggca atccatctgg gaggcccatc tggggttagc gttacccctg
     1501 acgagctata cctccctgtt gactttgatg agctgaaagt ccacggcata ccacttggtt
     1561 cgggaggagt c
//
LOCUS       DAOOQX010000525         1570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_103612_length_1570_cov_3.322772, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000525 DAOOQX010000000
VERSION     DAOOQX010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103612_length_1570_cov_3.322772"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..>1570
                     /locus_tag="PLX53_08060"
     CDS             114..>1570
                     /locus_tag="PLX53_08060"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOY73071.1"
                     /translation="MQMSFQFTILIPLIPLISFLIIGFLYSKMSNKLAGIWGSVSIFS
                     SFLLSLFTAYRYFFEVGRVNGVYPKILGFDFEWLRFSDSLSAHLGTLIDPISVMMLVV
                     VTTVSLMVHIYSIGYMKGDRGFNRFFAFLSLFSFSMLGLVLSTNIFEIYIFWELVGVS
                     SYLLIGFYYEKNSAVAASKKAFIVTRFADLFFLIGILILSFYTKTFDFISLTDPSNIA
                     VMQTGSITFMGLSVTTWALLLIFIGGAGKSAMFPLHIWLPDAMEGPSPVSALIHAATM
                     VVAGVFLVARLFPIYALGSDQALQVIGYVGAFTSLFAAVIAMTQMDFKRVLAFSTISQ
                     IGYMMVGLGVSGYGNEAGLGYMASMFHLFTHAMFKALLFLCAGSVIHVVHTNFINEMG
                     GLRKHMKITHITFLIATLAIAGFPGFSGFFSKDEIITAAYHNNKIIFYTELLVAGITA
                     FYMFRLYFRVFWNQEASYEHKPHEVEKTMTIPLIIL"
BASE COUNT          363 a          351 c          351 g          505 t
ORIGIN      
        1 attgccgttg cagcagctga ggctgcagtt gccattgcta ttattatcaa catttaccgc
       61 cgatttaagt ccatcaatat tgaaaatgta agtgaaatga aatactaacc actatgcaga
      121 tgagcttcca gtttaccata ctaatcccgc ttattccgct tatcagcttt ttgattatag
      181 gatttctata cagtaaaatg tccaataagc tggctgggat atggggttca gtttctattt
      241 tttcatcgtt cctcctgtcg ttgtttacgg cctatagata tttttttgag gttggcaggg
      301 tcaacggggt gtaccctaaa attttagggt tcgattttga gtggctaaga tttagcgact
      361 cgctgtcggc ccacctgggc acactgattg accccatttc ggtgatgatg ctggtggtgg
      421 ttaccactgt ttcgctgatg gttcatatct acagcatcgg ctacatgaag ggcgacaggg
      481 gatttaaccg cttttttgct ttcctctcgc tattcagctt ttccatgctg gggttagttc
      541 tatcaaccaa tatttttgag atatacattt tctgggagct ggttggtgtt tcgtcctacc
      601 ttctaattgg attttactat gaaaagaatt ccgcggtagc ggcttcaaag aaagccttca
      661 tcgtaacccg atttgctgac ctcttctttt taataggaat tctaatccta tcgttttaca
      721 ccaagacgtt cgatttcatt tcgctaaccg accccagcaa cattgcggta atgcaaaccg
      781 gaagtataac ctttatgggg ctaagcgtaa ccacttgggc attgctgctt attttcattg
      841 gaggagcagg taagtcggcc atgtttccgc ttcatatctg gcttcccgat gccatggagg
      901 ggccatcccc ggtgtcggcc ctaattcatg cggcgaccat ggttgttgcg ggtgtattcc
      961 tggtagcccg gctttttccg atttatgctt taggttctga tcaagcactt caggttattg
     1021 gctacgtggg cgccttcacc tccctgttcg cagccgttat tgccatgaca caaatggact
     1081 ttaagcgcgt tttagctttc tccaccatat cccaaattgg ctatatgatg gtgggattgg
     1141 gagtttcggg ctacggaaac gaagcaggcc tgggctacat ggcatcaatg ttccatttgt
     1201 ttacccacgc catgttcaaa gcgcttttgt tcctttgtgc cggatcggtt attcatgttg
     1261 tccacaccaa ctttatcaat gaaatgggcg ggctgcgaaa gcacatgaag ataacccata
     1321 ttacattttt aattgcaact ctagcaattg ccggattccc gggcttctca ggattcttca
     1381 gcaaggatga aatcattact gctgcatacc ataacaacaa gattattttt tacacggaat
     1441 tgctggtggc aggaattact gccttctata tgttccggtt gtactttagg gttttctgga
     1501 accaggaagc tagctatgag cataagccac atgaagtgga gaaaaccatg accattccct
     1561 tgattattct
//
LOCUS       DAOOQX010000526         1567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_103957_length_1567_cov_2.629630, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000526 DAOOQX010000000
VERSION     DAOOQX010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103957_length_1567_cov_2.629630"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..751)
                     /locus_tag="PLX53_08065"
     CDS             complement(158..751)
                     /locus_tag="PLX53_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73072.1"
                     /translation="MKKTVLLGFAALSMVAVLSGCGKVPQEKIDAANAAIDSAKMVEA
                     DVYVAADFAAVQDSMSAVMADIEVQKSKLFKKFGPANEKLDRILSQAHQIQANAVTKK
                     QEVKVEVEGLMTEVTTLIDQDKTLMKKAPRGKEGAAVLEQIKTEIAAIEASVAEAKTM
                     YDNGKYMDASNKLKAAKDSATKINTELTDAIAKVKKR"
     gene            complement(998..1387)
                     /locus_tag="PLX53_08070"
     CDS             complement(998..1387)
                     /locus_tag="PLX53_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOY73073.1"
                     /translation="MVPENAETFSTLVLGASCKAGRFSYLAIKSLVKHGVRVYAIGSR
                     PCFVDGVQVHAEKINLTNLHTVTLYLNSQNQTTYYDYILSLKPKRIIFNPGAENPELV
                     RLAHENRIDYVFGCTLVMLNDGSFFKS"
     gene            complement(1393..>1567)
                     /locus_tag="PLX53_08075"
     CDS             complement(1393..>1567)
                     /locus_tag="PLX53_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOY73074.1"
                     /translation="QRPKLMDIIPNMQLTLASVLKMDMEDISIKATTTEKLGFVGNEE
                     GVTAYAVVLIQKD"
BASE COUNT          442 a          312 c          310 g          503 t
ORIGIN      
        1 gcaaaattag catacattgg ctaatctatc aatattacta ggatattcgt tgaggtgaaa
       61 atcctcgtcc aatattaaaa catggtaact aaataataat agcctataaa aaaccccgaa
      121 gtttaacaac cccggggttt cggaaaatcg aattatacta tctctttttt accttagcaa
      181 tagcatcggt aagttcggtg ttgattttag tagcgctatc cttagcagct ttcagcttgt
      241 tggaagcatc catgtacttt ccattgtcgt acattgtttt ggcttcagca acgctagctt
      301 cgatagcggc aatttcggtt ttgatttgtt caagaacagc agcgccttcc tttccacgag
      361 gagctttttt catcaaagtt ttgtcctggt cgattaaggt ggttacttcg gtcattaaac
      421 cttcaacttc taccttaact tcctgcttct tggtaacggc gttagcttga atctgatgag
      481 cctggctcag aattctgtca agcttttcgt tagcaggtcc aaatttcttg aaaagtttgg
      541 atttttgaac ttcgatgtcg gccataacag cgctcatcga atcttgaact gctgcaaaat
      601 cggctgcaac atatacatca gcttcaacca ttttggcaga gtcaatagct gcattggcag
      661 catcaatttt ctcctggggc accttgccac aaccagaaag aactgcaacc atgcttaagg
      721 ctgcgaatcc tagtaaaact gtctttttca taatcgaatt gtttgggtta atgaagttaa
      781 atatctattg gtaattgtta tttaccgatg caaatttaca taagttttaa gcttatgtat
      841 taaaaaaaca taaatatttt aacaaaattt taaaaacatg gcttttctcc ttggcaagtg
      901 ctattcgtaa atagctgaaa ttaagttatt tatcaatttt aataattagt agcatggaat
      961 ttgccagcag ggttgctgca gaatgtttaa tgggaatcta tgatttaaaa aaactcccat
     1021 cgttaagcat aactaaggtg caaccaaaaa catagtctat tctgttttca tgtgctaatc
     1081 tcaccagctc ggggttttca gcaccgggat taaaaataat ccgtttgggt tttaggctta
     1141 aaatatagtc gtagtaggtt gtctgatttt gactgttcag gtaaagggtc accgtatgga
     1201 ggttggtgag gttaattttt tctgcatgta cttgaactcc gtcaacaaag catggcctcg
     1261 agccaattgc atagacccta accccgtgtt tcacaaggct tttaatggcc agataggaaa
     1321 acctccctgc tttacagctg gcccccagaa caagggtaga aaacgtttct gcattttcag
     1381 gtaccatgtg ggtcaatcct tttgaatcag gacaacggca taggcagtaa cgccctcttc
     1441 atttccaaca aatcccaact tttcggtggt tgtggcctta atagaaatat cctccatgtc
     1501 cattttaagg acggatgcca gggttagctg catgttggga atgatgtcca taagcttagg
     1561 gcgttgg
//
LOCUS       DAOOQX010000527         1567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_103988_length_1567_cov_2.270503, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000527 DAOOQX010000000
VERSION     DAOOQX010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103988_length_1567_cov_2.270503"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1567)
                     /gene="ftcD"
                     /locus_tag="PLX53_08080"
     CDS             complement(<1..>1567)
                     /gene="ftcD"
                     /locus_tag="PLX53_08080"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOY73075.1"
                     /translation="EAVEGVKLIDVDPGKATNRTVVTFVGTPVEVVEAAFRAVAKAKE
                     LIDMSRHHGAHPRFGATDVCPLVPVANITMDEVVEYARALAKRIGDELGIPVYCYEYA
                     ALEEKRRNLASCRAGEYEGLPKKLQDPEWKPDFGPAQFLPKTGAIAVGARNFLVAYNV
                     NLNTTSTRRANAVAFDIREKGRPMRVGNPVTGKIATDENGKEIWIPGTLKACKAIGWF
                     IEEYGIAQVSINLTDITVTPVHVAFEEASKKAQERGLRVTGSEIVGVVPLSAMLEAGK
                     YFLRKQQRSVGIPDREIIRIAVKSMGLDELYPFDPDKKIIEYILEEKGTRQLAGMTLE
                     SFSRETASESPAPGGGSISAAMGAFGAALAAMVANLSAHKPGWDDRWEEFSTWAEKGK
                     YYMEQLLALVDEDTRAFNRIMEAYGLPKGTDLEKAARSKAIQEATIQAIEVPFRVMNL
                     CYESLEVIRAMARQGNPSSVSDAGVGALAARSAVLGAFLNVKINSSGLKDKALVKDYL
                     DRGNQIASKTIAME"
BASE COUNT          316 a          480 c          391 g          380 t
ORIGIN      
        1 cctccattgc aatcgttttc gatgcaattt ggttgcccct gtccaggtaa tccttaacca
       61 acgccttgtc cttcagcccg ctggagttga tttttacatt caggaatgct cccagcacag
      121 ccgatcgggc agccagcgcc cctacaccgg catcggaaac ggagcttgga ttgccctgcc
      181 gggccatagc cctgattacc tccagcgatt cgtagcaaag gttcatcacc ctaaagggaa
      241 cctctatggc ctgaatggtt gcttcctgaa ttgccttgct gcgtgcggcc ttctcgaggt
      301 cggttccctt gggaagcccg taggcctcca ttatccggtt aaaggctcgg gtatcctcgt
      361 caactagcgc tagcagctgc tccatgtagt attttccctt ttctgcccaa gttgaaaatt
      421 cctcccaccg gtcgtcccat ccgggtttgt gggccgaaag gtttgccacc atggcggcca
      481 gcgctgcccc aaaagcgccc atggccgctg aaatggaacc accgccgggc gcgggcgatt
      541 cggaggctgt ttcgcggcta aaactttcaa gtgtcattcc tgcaagctgc cttgtgccct
      601 tttcctcgag aatgtactcg ataatctttt tatcggggtc gaacgggtag agctcatcaa
      661 gccccatgga cttaacggca atccggatga tttccctgtc ggggatgccc accgatcgct
      721 gctgcttacg aaggaagtac ttcccggcct ccagcatggc gctcaggggc acaacgccca
      781 caatctcgga gccggtaacc cgtaatccac gttcctgagc ctttttactt gcctcctcaa
      841 aagccacatg caccggcgta accgttatgt cagttaggtt gatggatacc tgtgcaatgc
      901 cgtactcctc aatgaaccag ccgattgcct tgcacgcctt tagcgttccg ggaatccata
      961 tctctttccc attctcatcg gtggcaattt taccggtaac ggggttccca acccgcatgg
     1021 gacgaccctt ctcacgaata tcgaatgcca cggcattggc ccttcgggtt gaggtggtgt
     1081 taaggttgac gttgtaggcc accaggaaat tccttgcacc aacggcaata gcgccggttt
     1141 tcgggagaaa ttgcgcaggg ccgaagtcgg gtttccactc cggatcctgc agctttttag
     1201 gaagtccctc atactctccc gccctgcagc tggccaggtt gcgacgtttc tcctcaagcg
     1261 cagcatactc atagcagtaa accggaatgc ccagctcgtc gccaatcctt ttggccagcg
     1321 cccgggcata ctccaccacc tcatccatgg tgatgttggc cacaggtact agcgggcaaa
     1381 catcggtggc gccaaaccgg ggatgtgcgc cgtggtgcct gctcatgtca ataagctcct
     1441 ttgctttggc aactgcccgg aatgccgctt caaccacctc aacgggggtt ccaacaaagg
     1501 ttacaaccgt acggttggtt gccttgccag ggtcaacatc tataagctta acaccttcaa
     1561 cagcttc
//
LOCUS       DAOOQX010000528         1556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_105195_length_1556_cov_2.280480, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000528 DAOOQX010000000
VERSION     DAOOQX010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105195_length_1556_cov_2.280480"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(423..>1556)
                     /locus_tag="PLX53_08085"
     CDS             complement(423..>1556)
                     /locus_tag="PLX53_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOY73076.1"
                     /translation="IFLQQYWWFIVSLLGALLVFLLFVQGGQTLIYTLGKNNTERTML
                     VNVLGRKWEFTFTTLVTFGGGFFASFPLFYSTSFGGAYWVWMAILFCFTLQAISYEFR
                     TKPNNFLGQKTYEVFLFLNGLLGTILLGTAVATFFTGSQFSVNKLNLVSSGNPVISSW
                     ETPWHGLEAALNFHNLSLGLAVFFLARTLAILYFMNSVNHDVILERSRKHLLYNAIPF
                     LVFFLTFVIWLMVRDGFAVNPSNGAVFMEKYKYLHNLLQMPAVLVLFLVGVVLVLWGI
                     GKSLLSKYSKGIWFAGFGTVFTVLALFLIAGYNNTAFYPSTFNIQHSLTIYNSSSSKF
                     TLTVMSIVSILIPFVVAYIWYAWKSLNVKKIDEDEIKTESHLY"
BASE COUNT          492 a          332 c          360 g          372 t
ORIGIN      
        1 aagattagca gaagcttaag gcggttactt ctaaccagcc tgttgagctt cagcaacctg
       61 tatatactag gcattatccg aaggatgttt cctctctgta cgcctaattg cccataggga
      121 caacaggtag gttaggtaaa tcaacaccgg ccagcttaac agccataaaa ttccggaaag
      181 gtatttcatc ggaaaatatt tattaggtta attgctatag gttacaattt tatcttttcg
      241 tttcctccta cccaaaaagc tcatcaccca acatatttca tccgtagttg aatcttcctg
      301 gcttataaac cacagctgag tgctagagat gccacctgaa tgttttactt gccttgatat
      361 tcaaagtaaa gatgaacggg tagataccat agaaacatca ttgtttaaag taaagtccaa
      421 agttagtaga gatgcgattc ggtcttaatt tcgtcctcat caattttttt cacattcaag
      481 cttttccagg cgtaccagat gtaagccacc acaaagggaa taagtatgga aacaatgctc
      541 atcaccgtaa gggtgaactt gctcgacgag ctgttgtaga tggtaagaga atgctggata
      601 ttgaaagttg aagggtaaaa ggcggtgttg ttgtagcctg caatcaggaa aagagccagg
      661 acggtgaaaa cggttccaaa accggcaaac caaatacctt ttgaatattt ggaaagaagc
      721 gacttaccaa ttccccaaag caccagcacc acgccaacaa ggaaaagaac cagcacagct
      781 ggcatttgca acaagttatg caggtacttg tatttctcca tgaaaacagc gccgtttgaa
      841 ggatttacag caaatccatc ccttaccata agccaaatta caaaggtgag gaagaatacc
      901 agaaaaggga tggcgttgta gagcaggtgt tttcgggagc gttcaaggat gacatcatgg
      961 ttaacgctgt tcatgaagta taggattgcc agcgttcggg ccagaaagaa aacagccagc
     1021 cccaacgaaa ggttatgaaa gtttagagca gcctcgagtc catgccatgg ggtttcccag
     1081 gacgagatta cggggttgcc cgagctgacc aggttgagct tgtttaccga gaattgcgat
     1141 ccggtaaaga aggtggccac ggctgtgccc aaaaggattg tacccagcag cccgttaagg
     1201 aaaaggaaca cttcgtatgt tttctgcccc agaaagttgt tgggtttggt ccggaattca
     1261 taggaaatag cttgaagggt gaagcagaat agaatggcca tccaaaccca gtaggcacct
     1321 ccaaagctgg tagagtagaa tagcggaaac gatgcaaaaa aacctccacc aaaggtaacc
     1381 agcgtggtga aagtaaactc ccacttgcgg cccagcacat tcaccagcat ggtgcgctcg
     1441 gtattgttct taccaagggt ataaatcaag gtttgtccac cctgaacaaa tagcaagaat
     1501 actagtaagg cacctaaaag cgaaacgatg aaccaccagt attgctgcag gaatat
//
LOCUS       DAOOQX010000529         1554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_105322_length_1554_cov_3.885257, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000529 DAOOQX010000000
VERSION     DAOOQX010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105322_length_1554_cov_3.885257"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="PLX53_08090"
     CDS             complement(<1..127)
                     /locus_tag="PLX53_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOY73077.1"
                     /translation="MAKKVVASTQQKGAKNFTRCIKMVKSPKTGAYTFKEEMVANT"
     gene            complement(135..317)
                     /gene="rpmG"
                     /locus_tag="PLX53_08095"
     CDS             complement(135..317)
                     /gene="rpmG"
                     /locus_tag="PLX53_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOY73078.1"
                     /translation="MAKKGNRVQVILECTEHKESGMPGTSRYITTKNKKNTPDRLERR
                     KYNPILKRVTVHREIK"
     gene            complement(330..572)
                     /gene="rpmB"
                     /locus_tag="PLX53_08100"
     CDS             complement(330..572)
                     /gene="rpmB"
                     /locus_tag="PLX53_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOY73079.1"
                     /translation="MSRVCQITGKKKMVGNNVSHSKRRTKRRFYPNLKKKRFFLEEEN
                     RWVTLKVSAAAIRTINKNGLKATLNKAVEKGILKTY"
     gene            complement(636..>1554)
                     /locus_tag="PLX53_08105"
     CDS             complement(636..>1554)
                     /locus_tag="PLX53_08105"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HOY73080.1"
                     /translation="VPSSCTVLPNYFGTAPGMLFKKGIHMLVSLPGVPFEMKALFENE
                     VTPLLKQLPVEGVIIHKVIHTFGIAESNLSDVLNEFEKSISPSIKLAYLPDPSGIRLR
                     LSAYGNDRKLLSQQVDEAIEKLRATIPQYIFGYGETSLPEVVGKMLLSCNATVSVAES
                     CTGGKISHLIVEIPGASSYFNGGVVAYSNQVKENLLGVKHQTLIKYGAVSREAVEEMA
                     VGSLNLLSTDYSIAVSGIAGPDGGTPDKPAGLVWIAAASVNSLEVEKYIFSNDRSINI
                     QRASYSALNMLRKLLLKEHPELKNIFEKV"
BASE COUNT          419 a          331 c          304 g          500 t
ORIGIN      
        1 cggtattggc aaccatttct tccttaaagg tgtaagcacc tgttttgggt gattttacca
       61 ttttaatgca acgggtaaaa tttttagcac ccttttgctg cgtagaagca actacttttt
      121 ttgccatggc tctactattt tatttcacga tgaacagtta ccctcttaag aataggattg
      181 tacttgcgac gttcaagcct atcaggggtg tttttcttat tctttgtagt tatatacctg
      241 gaagttcccg gcattccgct ttccttgtgc tcagtacatt caagtatgac ctgaaccctg
      301 ttacccttct tagccatttg ttaacagatt tagtaggttt taagaattcc tttttctaca
      361 gctttattaa gcgtagcttt cagtccattc ttattgattg ttcgtattgc agcagcagaa
      421 accttgaggg taacccaacg attttcctcc tcaaggaaaa accttttttt cttgagatta
      481 gggtaaaatc tgcgcttagt cctcctttta gaatgggata cattgtttcc caccattttc
      541 ttctttccgg ttatttgaca aactctcgac atcgtaatat tgttctaagt aagttttcta
      601 aacaggtcgc aaatttcggc aaaattatga atttatcaaa ctttttcaaa aatatttttc
      661 aactccgggt gttcttttag cagtaacttt cgaagcatgt tcagcgctga gtatgatgcc
      721 ctttggatat ttatggatcg gtcgttgctg aaaatatatt tttcaacttc cagggagttt
      781 accgaggcag ctgcaatcca tacaagccca gctggtttat ccggtgttcc tccatcagga
      841 cctgctattc ccgaaacggc tatggagtaa tctgtggaaa gtagatttaa agagccaacc
      901 gccatttctt ctactgcctc acgactaaca gcaccatact taattagtgt ttgatgtttc
      961 actccaagga gattttcctt tacctggttg gagtaggcga caacaccgcc attaaaatag
     1021 ctagaagcac ccggaatttc tacgataagg tgtgaaattt ttcctcctgt gcagctctcc
     1081 gctacagaaa ccgttgcatt gcagcttaat agcattttcc caaccacttc aggaagtgaa
     1141 gtttcaccat acccaaagat atactgagga attgttgccc ggagtttttc tatagcttca
     1201 tcaacctgtt gtgataagag cttcctatcg tttccataag ccgaaaggcg aagtcgaatg
     1261 cccgaagggt caggcagata ggcaagctta atggaaggtg aaatgctctt ttcgaactca
     1321 ttaagcacat ccgaaaggtt tgactcggca atgccaaagg tatgtataac cttatgaata
     1381 ataacacctt caactgggag ttgctttagc aagggagtaa cctcattttc aaaaagtgcc
     1441 ttcatttcga agggtacacc tggaagggaa accagcatat ggattccctt tttaaacaac
     1501 atgccaggag ctgttccaaa atagttaggt agtacagtgc aggaagatgg aaca
//
LOCUS       DAOOQX010000530         1554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_105333_length_1554_cov_3.132755, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000530 DAOOQX010000000
VERSION     DAOOQX010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105333_length_1554_cov_3.132755"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1554
                     /locus_tag="PLX53_08110"
     CDS             <1..>1554
                     /locus_tag="PLX53_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOY73081.1"
                     /translation="ADTNFRSHSVNLILTIRNPFTIDEKGNKVKSNFKKFKVKRVFIY
                     PNFEPLEYLTYQQQATLDTVYYNDIQFIFSGDAGVNLAVIYENILIRPGLTYSSEAVK
                     KSQANLSSIRLFKFVNISFKEAKEAEFPISPFDFLDEDSASSDSTEYAYLDCYIQLTQ
                     NTLQSYQVELVGTNTTGSLGAEANLSYQHKNLFRGAEVFDFKLRGLVETAQQKINFDN
                     TLELGGSMGLSIPKYIGPFSSRVQVSRSAPKTQVTASYSYQHRPDYTRTIASLLFGYS
                     WRSGSFITHNVNPAEVNAITISRISDDFKAYIDTTSLKYSYISQIVTVSSYGFTFNNQ
                     NPQKPGSYTYLRYNFELSGNLLNLAFAAFNQPKNADGSYSIFNTNFSQFVRSDLNYTY
                     NQYVDANNSFAYRIFLGVGYPYGNSKALPFEKRYFTGGANGIRAWQARALGPGSYYQA
                     KERYPNRTADIKLEANLEYRYKLFWKVEGALFIDAGNIWSMPGIDDRKGATFHFDKFY
                     EQLALGSGL"
BASE COUNT          442 a          365 c          323 g          424 t
ORIGIN      
        1 ctgctgatac taatttccgt agccacagcg taaaccttat cctcaccatt cgtaaccctt
       61 ttacaattga tgaaaagggg aataaggtta agtcgaattt taaaaagttt aaagttaagc
      121 gggtgtttat ttatccaaat tttgagcccc tagaatactt aacgtatcaa caacaggcaa
      181 cgcttgatac ggtttactac aatgacatac agttcatttt ttcgggtgat gccggagtta
      241 acctggctgt tatttacgaa aatattctta ttcgacccgg cttaacctat tccagcgagg
      301 cagtcaaaaa atcacaagcc aaccttagct ccataaggct atttaaattt gtaaacattt
      361 cgtttaaaga ggctaaggaa gccgaatttc ctattagccc tttcgatttt ttggatgagg
      421 acagcgccag ctccgattcc accgagtatg cctatctcga ttgctacatt caacttaccc
      481 agaatacgct gcaaagctac caggttgaac ttgtgggaac taacaccacc ggttcgctcg
      541 gtgccgaagc taacctttcc taccagcaca aaaacctatt caggggtgcc gaggtgtttg
      601 attttaagct taggggcctg gttgaaacag cccagcaaaa gataaatttt gataacaccc
      661 tagaacttgg cggctccatg gggctgagca tacccaagta tattggccct ttttcttcca
      721 gggttcaggt tagccggtcg gctcccaaaa cccaggtaac cgcatcctac agctaccagc
      781 atcgccccga ttatactcga accattgcca gcttgctgtt tggctatagc tggagaagcg
      841 gaagctttat tacccacaac gtcaacccag ccgaggtgaa tgccattacc ataagccgta
      901 ttagtgatga ttttaaggcc tacattgata caacttcgct caagtatagc tatatcagtc
      961 agattgttac cgtgtcgagt tacggcttta cctttaacaa ccagaatccc cagaagcccg
     1021 gtagctacac ctaccttcgt tacaactttg aactttcggg taaccttctt aaccttgcct
     1081 ttgcagcctt taatcaacct aaaaatgccg atggttctta cagtatattc aatacaaatt
     1141 tttcacaatt tgtccggagc gatttaaact atacctacaa ccagtatgta gatgcaaata
     1201 actcgtttgc gtatagaatt tttcttggcg taggctaccc ctatggcaac tcaaaagcat
     1261 taccattcga aaaacggtac ttcaccgggg gcgcaaatgg aataagggcg tggcaggcaa
     1321 gagcgctagg acctggatca tactatcagg caaaagaacg ctatcctaac cgtacggccg
     1381 atatcaagct ggaggctaat ttagaataca ggtataagct cttctggaaa gtagagggtg
     1441 ccttgttcat agatgccggc aacatctggt ctatgcccgg aattgacgac aggaaaggtg
     1501 caacatttca tttcgataag ttttacgaac agctggcttt gggtagcggg ttgg
//
LOCUS       DAOOQX010000531         1554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_105371_length_1554_cov_2.385590, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000531 DAOOQX010000000
VERSION     DAOOQX010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105371_length_1554_cov_2.385590"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..>1554
                     /locus_tag="PLX53_08115"
     CDS             58..>1554
                     /locus_tag="PLX53_08115"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HOY73082.1"
                     /translation="MTDSAGQGQQPLGEVYCLHQLSNGAILAGTSNGLVSVNLLKGSK
                     NLYFENLIIPSISARAIEEDVKGNFWLSTSKGITCYNPSTGQLASYTSDDGLLGNEFN
                     LLSSFQSPSGTIYFGGMQGISYFHPDSVMLNSHIPPLSFTKISLASVSSTKNIHPFGI
                     DEVRIPQGFSSLTIEFSALDFNHPEKNLYRYRLQGPGSSKWVELGNKHSITLTNFREG
                     VFRLEVMGANSDCVWTTEPLSLRIIVVAPFWRSRIAYTVYALLLLFVLIAYGSYRRRN
                     SLRIKRLLAEREDVMAKLELQKEELENKNKNITDSIHYAKRIQDAMLPTVDMFKSHIP
                     NSFILFKPKDIVSGDFYWVKETDSKCFVAVVDCTGHGVPGAFMSIIGIELLRNITEVE
                     AVEDTAEVLNRLSASVHETFSSVSYPNESGLKVKDGMDVTFCTFDKQYDIMQFTGAFS
                     DLYIIRDGNIIEVKGDRFSVGMADDAGRYMFSSSYIPLMPDDMIYLFTD"
BASE COUNT          434 a          300 c          361 g          459 t
ORIGIN      
        1 gcaaatggtt tatcccgtat atcggacaat tgttcaagcg tgattaatat aacggttatg
       61 accgattccg ctggtcaagg acaacaaccc ctaggtgaag tttactgctt gcaccagcta
      121 tcaaatggag caattctggc tggtaccagc aacgggttgg ttagcgttaa cttgttgaaa
      181 gggagcaaaa acctttattt cgaaaacctt atcatcccat caatatccgc aagggcaatt
      241 gaggaggatg taaagggtaa tttttggctt agcaccagca agggtattac ctgctataac
      301 ccatcaacag ggcaacttgc cagctataca tctgacgacg gcttgctggg gaacgagttt
      361 aacttgttgt ctagcttcca gtcgccttcg ggaacaatat actttggagg gatgcaggga
      421 attagctact tccatcccga ttcggtaatg cttaatagcc atattcctcc attaagtttt
      481 actaaaatat ccttagcgag cgtaagcagt accaaaaata ttcacccttt tggtatagat
      541 gaagtaagaa tcccgcaggg atttagctca ctgactattg aattttcggc gctggatttt
      601 aaccatcccg agaaaaattt ataccggtac aggctacagg gacctggtag tagcaagtgg
      661 gttgaacttg gcaacaagca ttcaatcacc ttaacgaact ttagagaggg tgttttcagg
      721 cttgaggtta tgggggctaa cagcgattgt gtttggacta ctgaaccttt atccctccgg
      781 attattgttg ttgctccttt ttggcgatca cgtattgcat acactgtcta tgccttgctt
      841 ttactattcg tattgatcgc ttatggaagc tatcgaagaa gaaattcact aaggataaaa
      901 aggttgcttg cagagcgcga ggatgttatg gccaagcttg aacttcaaaa agaggagctg
      961 gaaaataaga acaaaaatat aaccgatagc attcactatg ctaaaaggat tcaggatgcc
     1021 atgcttccta ctgttgacat gtttaaaagc catattccaa attctttcat cctctttaaa
     1081 cctaaggata ttgtaagtgg ggatttttat tgggtaaagg aaactgatag taaatgcttt
     1141 gttgccgttg ttgactgcac tgggcacgga gttcccggcg cattcatgtc aatcattggc
     1201 attgagctgc ttagaaacat caccgaggtt gaggcagttg aggatactgc tgaagtgctg
     1261 aataggctta gcgctagtgt tcatgaaacc ttttcgtcgg ttagctaccc taacgaaagt
     1321 ggacttaagg ttaaagatgg gatggatgtt accttttgca cctttgataa gcagtatgac
     1381 ataatgcagt ttacaggggc gtttagcgat ttgtacatta tccgtgatgg taatattatc
     1441 gaggttaagg gcgacagatt ttctgttggt atggctgatg atgcagggcg ctacatgttt
     1501 agcagctcat acataccttt aatgcccgac gatatgatat acctttttac cgat
//
LOCUS       DAOOQX010000532         1553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_105479_length_1553_cov_2.339786, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000532 DAOOQX010000000
VERSION     DAOOQX010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105479_length_1553_cov_2.339786"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PLX53_08120"
     CDS             <1..293
                     /locus_tag="PLX53_08120"
                     /inference="COORDINATES: protein motif:HMM:NF027968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HOY73083.1"
                     /translation="LVELKKSTPVLAAGDRGADIFRIKSNADSAVFAFTRKNESQALF
                     AIFNLSANQQIATLTGNDYAGTYTNVMTGEKFVFQGGEEISLKPWEFYIYCN"
     gene            546..>1553
                     /locus_tag="PLX53_08125"
     CDS             546..>1553
                     /locus_tag="PLX53_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOY73084.1"
                     /translation="MHYSVPVSNKIHWIGVNDRRKRLFENLWPLERGVSYNSFLIVDE
                     KTALVDTIDGTAVGNYIERIEQLLNGRPLDYLIVNHMEPDHSGEVKAIIDRFKGVKLV
                     GNVKTFKIVEAYWGITEGLIDVEDGRTLDLGYHKLKFLMTPWVHWPETMMTYDTTDQI
                     LFTGDAFGSFGAFEGGIFDDELDPLYFEDEARRYYSNIVGKYSNMVQKAIAKLEGIPV
                     RTIAPTHGPVWRSNPTIIMDWYNRWSRFEAAKGAVIIFASMYGNTEDIADYLARRLCE
                     NGVRNIRVFDVSKTHISYLISEIWKFNGLVLGSCAYNSQMFPLMEQLTHELLHIGLKS
                     KH"
BASE COUNT          456 a          317 c          329 g          451 t
ORIGIN      
        1 agttggttga gcttaaaaaa tctacgcctg ttctagccgc cggggacagg ggtgctgata
       61 tattccgaat caaaagcaac gccgacagcg ctgtattcgc ttttacacgg aagaatgaaa
      121 gtcaagctct atttgccatt tttaacctct cggctaatca gcaaatcgca actcttactg
      181 gcaacgacta cgctggtacc tacaccaacg taatgacagg tgaaaaattt gttttccaag
      241 gcggggagga gatttcatta aaaccatggg aattttatat ctactgtaat taaaatttca
      301 aatatttgtg attcagggcg ttagtagtaa cgcccttttt ctttaggtaa attgccgcat
      361 caacatgaac gtaatatatt gtagagtttg gtgcatatac tgcaatgacc catttatcat
      421 aaacccacac cgtgctaaaa tcttctttcc cttacaacat aaaataactt tgtcctcaaa
      481 tcaatagagc ctccagctaa aattgctatt tttgtgcttc agaatgatat taaactctat
      541 ttcttatgca ctattcagtt cctgtaagca ataaaatcca ttggattggt gttaacgacc
      601 gccgaaagag gttgtttgag aatttatggc ctctcgaaag gggtgtatcc tacaactcat
      661 ttttaatagt tgacgaaaaa acagcgctag ttgatacaat tgacggtaca gccgtaggca
      721 actatatcga acgcattgag cagctgctaa acggacgccc tctcgactat ttaattgtaa
      781 accacatgga acccgaccat tcgggcgagg tgaaagccat tatagatcgc tttaagggcg
      841 ttaagctggt agggaacgtt aaaaccttta aaattgttga agcatactgg ggaattacag
      901 aaggtttgat tgatgttgag gatggtagaa ctcttgattt gggataccat aagctgaaat
      961 tcctgatgac accttgggta cattggcccg aaaccatgat gacctacgat acaaccgatc
     1021 aaatactttt tacgggtgat gcctttggta gttttggcgc ttttgaaggt ggaatttttg
     1081 acgatgagct tgaccctctt tactttgagg atgaagctcg ccgctactac tccaatattg
     1141 ttgggaagta cagcaatatg gttcaaaaag ccattgccaa acttgaagga atccctgtta
     1201 gaaccattgc accaacccat gggcctgtct ggcgttcaaa cccaaccatt attatggact
     1261 ggtataatag gtggagtcgc tttgaagctg ctaaaggagc cgttatcata tttgcctcaa
     1321 tgtatggcaa cactgaggat attgccgatt acctggcccg caggctttgt gagaatggtg
     1381 ttagaaatat tagggtcttc gatgtctcca aaacacacat tagctacctg attagcgaga
     1441 tatggaagtt taacggtctt gttttaggat cgtgtgccta caactctcaa atgtttccac
     1501 tcatggaaca gcttacacac gaactgcttc acattggact gaaaagcaaa cat
//
LOCUS       DAOOQX010000533         1550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_105748_length_1550_cov_3.919732, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000533 DAOOQX010000000
VERSION     DAOOQX010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105748_length_1550_cov_3.919732"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1550
                     /locus_tag="PLX53_08130"
     CDS             <1..>1550
                     /locus_tag="PLX53_08130"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOY73085.1"
                     /translation="LKPGNVPSLVYQPEVPFTQPKEFEVVGLEAENVIIGYRFRGANS
                     PDNDMLDLISAILYNGRAGLIDLNINKKQLTLGCQAYNRAMADYSILQFSGRNKKGQS
                     LEEVKNLLLQQVELLKKGDFPDWMLEAAVNNVKLRQMSQLESARGRASLMYSAFLNNI
                     EWADEVAYISRLEKITKEQVVRFANDNLGSNYVVVYKRQGKPEEVAKVAKPPITPIYI
                     NRDAESDFLKMIKNSTVKPIEPRFIDYDKDITKFKLNTNVEVLYTKNAENSTFNLTLY
                     YPFGRLANPLLTHAADYIQLLGTSKLTAEQLSQEFYKLACNFNISVRDEEMLISLTGL
                     SDNADKAFELMENLLADCQPNSDALSMYITNQLKSRDDAKKRQQSVFSALVSYATYGE
                     ENPQRLILSNEELKGLTAQPLVDLIREFTSYPHIILYYGPQDVKGLKKMLTEKHIQPK
                     NYKSLPVNKKFEETSTTTERVLFVNYDAKQSYLQTISKGELYSDNLYPVVTLYNEYFG
                     GGMNAIVF"
BASE COUNT          460 a          365 c          343 g          382 t
ORIGIN      
        1 gcctgaaacc tggaaatgtt ccttccttgg tgtatcaacc cgaggtgcct ttcacccaac
       61 caaaggaatt tgaggttgtg gggttggaag ccgaaaacgt gattattggc taccgtttca
      121 ggggtgccaa ctcacccgat aacgatatgc tcgaccttat ttcggcaata ctttacaacg
      181 ggcgtgccgg attaatcgac ctcaacatca acaagaagca gctgacgctt ggatgtcaag
      241 cctacaacag ggctatggcc gactactcaa ttcttcaatt ttcagggcgt aacaaaaagg
      301 gacagtcgct cgaggaggtt aaaaacttac tgctccagca ggttgagctg cttaaaaagg
      361 gcgattttcc cgactggatg ctcgaagctg ccgttaacaa cgtaaagctg cgccaaatgt
      421 cccaactcga aagtgcacgc ggtagggcta gtttgatgta ctcagccttt ttgaacaaca
      481 ttgaatgggc cgatgaggtt gcctacattt ccagattgga aaaaattacc aaagagcagg
      541 tggttcgctt tgccaacgat aacctgggta gcaactacgt tgtggtttat aagcgacagg
      601 gtaagcccga ggaggttgcc aaggttgcca aaccgcccat aactccaatc tacatcaacc
      661 gcgatgccga atcggatttc ctgaaaatga ttaaaaactc taccgttaaa ccaattgaac
      721 caaggtttat tgactacgat aaggatatta ccaaattcaa gctgaacacg aacgtagagg
      781 tgctttatac caagaatgcc gaaaacagta ccttcaacct gactttatac tacccatttg
      841 gaagattggc taatccttta ctaactcatg cagccgacta catccagctg cttggaactt
      901 caaagcttac cgccgaacag ctaagccagg agttctacaa gctagcttgc aacttcaaca
      961 tttctgtcag ggacgaggag atgctaattt cgttgacggg cctgagcgat aatgccgata
     1021 aagcctttga gctgatggaa aaccttcttg ccgattgcca gcccaacagc gatgccctct
     1081 ctatgtacat taccaaccag ctaaaatcga gagacgatgc caagaaacgg cagcaaagtg
     1141 tgtttagcgc attggttagc tatgctactt atggagagga aaacccacaa cgcttaattc
     1201 tctcaaatga agagttgaag ggtcttactg cccaaccgct ggttgaccta atcagggagt
     1261 ttacctcata cccgcacatc attctttact atggacctca ggatgttaag gggttgaaaa
     1321 aaatgctcac cgaaaagcat attcaaccta aaaactacaa atcgttgccg gtgaacaaaa
     1381 agtttgagga gacttcaaca actaccgaaa gggtgctctt tgtaaactac gatgccaagc
     1441 aatcgtacct tcaaaccatt agtaagggcg agctttacag cgacaacctt tatcctgtgg
     1501 taacccttta caacgaatat tttggtggtg gtatgaatgc cattgttttc
//
LOCUS       DAOOQX010000534         1547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_106119_length_1547_cov_3.235925, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000534 DAOOQX010000000
VERSION     DAOOQX010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106119_length_1547_cov_3.235925"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..619)
                     /locus_tag="PLX53_08135"
     CDS             complement(116..619)
                     /locus_tag="PLX53_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY73086.1"
                     /translation="MKHIFTTLILTLSMLAGYSQEVAMQVLATAGGYYENTGAGLSLS
                     WTLGEPAYTTLTSSEVILTQGFQQGNLFGTNLEEIPTDVTAIRVYPNPVTSTVNIAVT
                     LPNAKGRATVEVYDIAGRMVKSTSAVMVGHEPYRLDVSNLPSGIYLLRVATESPAAVR
                     VVKLVKE"
     gene            777..1484
                     /locus_tag="PLX53_08140"
     CDS             777..1484
                     /locus_tag="PLX53_08140"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HOY73087.1"
                     /translation="MMNDVIRLLNEQHSQSTPLEVLKWFLQEYQGRIALSSSLGAEDQ
                     VLTDMVVKIDPATKIFTLDTGRLFYETYELMEKTCLRYGISISVYFPEAGRVERMVTE
                     KGINLFYESVENRKECCHVRKVEPLKKAFQGLDVWICGLRRSQTITRADGQLVEWDEE
                     NGLIKLNPLIGWSEDEVWSYILENHVPYNTLHDKGFPSIGCQPCTRAVMPGEDIRAGR
                     WWWENQDTRECGLHVKK"
BASE COUNT          402 a          352 c          396 g          397 t
ORIGIN      
        1 tgggctcttg cctcaacacc tatcagagtc accagcaatg ctgtaaaaag gcaactcctc
       61 actctcatgg ctatttatcc ttaaaagttt ttttattcca tttactacat agctactact
      121 ccttcaccag cttcaccacc cgcacggcag cgggagactc cgtggccacc ctcagcaggt
      181 agattccgga cgggaggttg gatacatcta gcctgtaggg ttcatgtccg accatcactg
      241 ctgatgtgct ctttaccatg cggccagcaa tgtcgtaaac ttcaaccgtg gctcgtccct
      301 tggcgttggg cagcgtaacg gcaatgttca ccgtgctggt cacagggttg gggtaaaccc
      361 gtatagctgt aacatccgtc ggtatttcct ccaggttagt tccaaagagg tttccctgct
      421 gaaacccctg ggtaaggatt acctccgaag aggttagcgt ggtgtaggcc ggttcgccca
      481 gcgtccagct tagcgacaga cctgcacccg tattctcata gtatccacct gccgtggcca
      541 gcacctgcat ggctacctcc tgggagtacc cggccagcat ggatagcgtt aaaatcagcg
      601 ttgtgaagat gtgtttcatc ctttttcact ttaaattgtt tagttgtttg ttgacttgta
      661 attagttttt acacaagtta atacattttt ctaaatgaaa gtcaagttat ttacataaaa
      721 atgatgagta tttttgacga gtggtatatt ttgttaacta tttcaatttc agagtaatga
      781 tgaatgatgt aattcgattg ctaaacgagc agcatagcca atcaacccct ctggaagtgc
      841 tgaaatggtt tcttcaggag tatcagggac ggatagccct ctcatcgagc cttggcgctg
      901 aggatcaggt gcttaccgac atggtggtga agattgaccc agctaccaaa atattcacac
      961 ttgacaccgg aaggcttttc tacgaaacct acgagctgat ggagaaaacc tgccttaggt
     1021 acggaatttc catcagcgtt tacttccccg aggccggcag ggtggagagg atggttacgg
     1081 aaaagggaat taacctgttt tacgaaagcg ttgaaaacag gaaggagtgc tgccatgtaa
     1141 gaaaggtaga gccactgaaa aaggcatttc agggacttga tgtttggatt tgcgggttaa
     1201 gacgtagtca gaccatcaca agagccgacg ggcagctggt ggaatgggat gaggaaaatg
     1261 gtcttatcaa gctaaacccg cttattggct ggtcagagga tgaggtttgg agctacatcc
     1321 ttgaaaacca cgtaccctac aacacgcttc acgataaggg tttcccaagc attggatgcc
     1381 aaccatgcac cagggcagta atgcccggag aggatatcag ggcaggacgc tggtggtggg
     1441 agaatcagga cacacgggag tgcgggctgc acgtaaagaa ataaaaaagc ccctgttggg
     1501 gcttatagat gaggtcccga gcggattcga accgctgtaa atggttt
//
LOCUS       DAOOQX010000535         1546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_106251_length_1546_cov_2.935614, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000535 DAOOQX010000000
VERSION     DAOOQX010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106251_length_1546_cov_2.935614"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLX53_08145"
     CDS             <1..311
                     /locus_tag="PLX53_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOY73088.1"
                     /translation="IPRVLDAGQCNDSYSLAIIALKLKEIFQLNDVNELPIAYNIAWY
                     EQKAVIVLLALLYLGVKNIHLGPSLPGFLSPNVTNVLVDNFGISGITTVDEDIKLLVG
                     "
     gene            409..735
                     /locus_tag="PLX53_08150"
     CDS             409..735
                     /locus_tag="PLX53_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOY73089.1"
                     /translation="MNNEFEKEKKFNFLDEVDYSTGGVVSKNVIKKPTGNISLFAFEK
                     GEGLSEHTAPFDALLQVIDGEAKVTINGKPHDLKAGESIIMPANSPHAVFAAERFKMV
                     LTMIKE"
     gene            1007..1312
                     /locus_tag="PLX53_08155"
     CDS             1007..1312
                     /locus_tag="PLX53_08155"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOY73090.1"
                     /translation="MNIITPENAEKVPFALNGRKMYSSPTAEIVHLTLKPGETVDRHS
                     NPFDVVFYMLEGEATIEIDGVKAILKTDCCLEVKRDKPRGMSNNTGKDVRILVFKLF"
BASE COUNT          475 a          348 c          330 g          393 t
ORIGIN      
        1 gaataccccg ggtgctggat gccgggcagt gcaacgactc ctactcgctg gccatcattg
       61 cccttaagct gaaggagatt ttccagctga acgatgtcaa cgagctgcca attgcctaca
      121 acatagcatg gtacgagcag aaagctgtta ttgtgctact cgccctgctc taccttgggg
      181 taaaaaatat ccatttaggc ccgtcacttc cgggtttcct ttcaccgaac gtaaccaacg
      241 tgctggttga caactttgga ataagcggta taactactgt tgacgaggat attaagctac
      301 ttgtagggta attcttaaaa tttcaaaaaa acaccatcct gtgtgcccgg gtattcaaga
      361 aaccgcacca ggatggttaa ttttaaaccc ttcaaaaaaa atacagccat gaacaacgag
      421 tttgaaaagg aaaagaagtt caactttctg gatgaggttg attacagcac gggaggcgtt
      481 gttagtaaaa acgtgattaa aaaaccgaca ggtaatattt cgctgtttgc attcgagaaa
      541 ggagaagggc ttagcgagca tactgcacca tttgacgccc tgctgcaggt tattgacggc
      601 gaagctaaag ttaccataaa cggcaagcca cacgacctaa aggccggaga atctataatc
      661 atgcctgcca acagccccca tgcagttttt gcagctgaac gcttcaaaat ggtccttacc
      721 atgatcaagg agtaaaccat catgaagcct ataaaaaatt caccagcctg aacacacgtt
      781 cgggttggtg gattctaaaa acattgtaag gagcaataat caaccctatt aggctacacc
      841 aatactcagt atttcgaagg gagtatttat ccggttttcc ctccaaagtt tttagcttaa
      901 ccttttacaa gctaaaaaca accatttgaa ccaccagtaa tcggattatt agtgatttaa
      961 aattcgtcat gcaacggttt tttttaattt taaaatgata gcaaacatga atataattac
     1021 tcccgaaaat gcggaaaaag tcccttttgc actcaacggg cgcaagatgt acagcagccc
     1081 tactgcagaa atagttcatc taacactaaa acctggcgaa acggttgata ggcactccaa
     1141 ccccttcgac gtagtgttct acatgctcga gggcgaggct accatagaaa ttgacggggt
     1201 gaaagccatt cttaaaaccg attgttgcct tgaggtaaag cgtgataagc ctcgtggaat
     1261 gagcaataat acaggaaaag atgtacgaat actcgttttt aagctgttct gagacgatag
     1321 ccgatgagta aaaataaaga ataatgataa aggataagat aaacttgtcc gcctcctggc
     1381 ggaacttgtc cgcctcccga tggaacttgt ccgcctcccg atggaacttg tccggctcct
     1441 gccgtattcc agattcaaaa tttaaaattt cagaacctgt ccgcctactg gcggattcac
     1501 ccttcgacag gtgttacagt gagtagttcg gctggctcac tataac
//
LOCUS       DAOOQX010000536         1541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_106827_length_1541_cov_2.984522, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000536 DAOOQX010000000
VERSION     DAOOQX010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106827_length_1541_cov_2.984522"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLX53_08160"
     CDS             <1..482
                     /locus_tag="PLX53_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY73091.1"
                     /translation="LNPRLTIGQALVEPVVVHGLAGKNEAKSRVLELLDRVKLPADSF
                     YRFPHEFSGGQRQRLVIARALTLQPKVLICDEIISALDIAIQVQILNLLEELKRDFNL
                     TYLFISHDLGVVRHISNRVIVLKEGTIDEEGPAEAIFAQPKSTYTKTLIAAMPQLLA"
     gene            complement(535..>1541)
                     /locus_tag="PLX53_08165"
     CDS             complement(535..>1541)
                     /locus_tag="PLX53_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOY73092.1"
                     /translation="LDSIKYYKKFLRSSFMAINPHDGHVKAYVGGPDFRHFKYDMVRQ
                     GKRQVGSTIKPFLYTLAMQEGYSPCMEVPNVPQTFVVGDTTWNPKNSGSTKYDGQMVT
                     LKWGLANSINNISAWVMKQFTPQIVADMINQLGIKTHVDPVPSIFLGTIDFSLFEMVG
                     AYGTFANKGIFIDPIFVTRIEDRNGNVLATFNTVKTEAISEQTAYLMINLLEAVVNQG
                     TGIRIRYKYQLPGKIAGKTGTSQNHSDGWFMGVTPNLVAGVWTGAEDRAVHFDNISMG
                     QGASMALPIFATFLQKVYANPSLGVSPNDDWDKPILKQNVNLDCNKQFDNEINEIELY
                     "
BASE COUNT          411 a          382 c          363 g          385 t
ORIGIN      
        1 cgttaaaccc acgcttaaca attggacaag ctcttgtaga acctgttgta gtccatgggc
       61 tggctggcaa gaacgaagca aaatcacgcg tgctggagtt gctggacagg gtgaagctac
      121 ctgctgatag cttctaccgt tttccacacg aattctcagg tggtcagaga caacggctgg
      181 taattgcccg tgcactaacc cttcagccca aagtgttgat ttgcgatgag attatttccg
      241 cccttgacat tgccatacag gttcaaattc tgaacctcct cgaagagcta aaacgggact
      301 tcaacctaac ctaccttttc atctcgcacg atttgggggt agttaggcat atcagcaacc
      361 gggtgatagt cctaaaggag ggaacaattg acgaggaagg ccctgctgag gcgatttttg
      421 cacagcccaa aagcacctac acaaaaacgc tcattgcggc catgccgcag ctactagcct
      481 gatgtaaccc gaaacgatca agcccaaacc tgctaccatg cacgaagcaa aaggctagta
      541 cagctcaatt tcgttaatct cattatcaaa ctgcttattg cagtcgaggt taacgttttg
      601 ctttaggatg ggtttatccc aatcatcgtt gggcgaaact ccaagcgatg gatttgcata
      661 taccttctgc aaaaaagtag caaaaattgg caaagccatg ctggcgccct gccccatgga
      721 aatattgtca aaatgaacag ccctgtcctc ggcgccggtc caaacgccag caaccaggtt
      781 tggagtaaca cccatgaacc atccgtcgga gtgattttgg gatgtccccg ttttcccggc
      841 gatttttccg ggcagctggt acttgtagcg tatgcgaata ccggtgccct ggttaacaac
      901 agcttcgagc aggttgatca tcaggtaggc tgtttgctcg cttatagcct cggttttcac
      961 cgtgttaaag gtagccagca catttccatt cctatcctcg atgcgggtta caaaaattgg
     1021 atcgataaaa atgcccttat tggcaaaagt gccgtaggcc cccaccatct cgaaaaggct
     1081 aaagtcaatg gtgccaagaa aaattgaagg aaccggatca acgtgagttt taatacccag
     1141 ctggttaatc atgtctgcaa ctatttgcgg ggtaaactgc ttcataaccc aggccgaaat
     1201 gttgttgatg gagttggcca aaccccactt aagggttacc atctgcccgt cgtacttagt
     1261 actgccggaa tttttggggt tccatgttgt atcgcccacc acaaaagttt ggggaacatt
     1321 tggaacctcc atgcatgggg aatacccctc ctgcatggca agtgtgtaaa gaaaaggctt
     1381 aatggtggag ccaacctgtc ttttcccctg cctaaccatg tcgtacttaa agtgtcgaaa
     1441 atcagggcca cccacgtatg ccttcacatg accatcgtgg gggttaatgg ccataaaact
     1501 cgaccgaaga aactttttgt agtacttgat ggagtcaagg g
//
LOCUS       DAOOQX010000537         1540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_106902_length_1540_cov_4.480808, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000537 DAOOQX010000000
VERSION     DAOOQX010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106902_length_1540_cov_4.480808"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="PLX53_08170"
     CDS             complement(<1..1235)
                     /locus_tag="PLX53_08170"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HOY73093.1"
                     /translation="MAARLWNVKFYLLGLVLLNSCSLAPAPPKAEVHPYQMVTCGDTR
                     IDPYFWLCYRDSQKVLDYLLAENAYTEAMMKSTMPLQEKLFNELKSRKKVEDVTVPAF
                     MNGYFYYTRFEEGKEYPINCRRKGNMDAPEEVILDENEVAAGQRYCQVASLDISPNNQ
                     ILAFGVDTLGKRLFTIYFKNLETGKLLPIKIDNTTGTIAWASDSQTLFYTQKDQTLRP
                     SRIYRHKISKNANPKDELVYYEADSTFSTHVVNSKSLEYIFIFSSSGSCSEYRYLKTS
                     TPEGKFKVFQPRMPGVEYTVEHSGNFFYIMTSWNAENNRIMETPLTSTRKESWRELVP
                     NRSDVLVMNVDIFKDFLVATERKDALIQVKVISLRDKSQHYIDFGEEVYTALPSVNSE
                     FNTSSFVYFYTSLTTPGVT"
BASE COUNT          407 a          345 c          334 g          454 t
ORIGIN      
        1 caggttactc ccggtgtggt gagcgaggtg taaaagtaaa caaatgatga agtgttaaac
       61 tcggagttta ccgatggtaa agcagtatat acctcctcgc caaaatcgat gtagtgctgc
      121 gatttatccc tgagtgatat caccttaacc tggattaaag cgtcttttcg ctctgtagca
      181 acaagaaaat ccttgaagat gtccacattc atgaccagca catcactcct gttcgggaca
      241 agttccctcc agctttcctt tcgggtagat gtcagaggcg tttccataat cctattgttt
      301 tcggcattcc agctggtcat aatgtagaaa aagttacccg agtgctctac ggtgtattcc
      361 acaccgggca tacggggttg aaatacctta aatttacctt cgggtgtgct ggtctttagg
      421 tagcggtact cggagcatga accgctgctg ctgaatataa atatgtattc caacgatttg
      481 gagttaacaa catgagtgct aaatgttgaa tcggcttcat agtaaaccag ctcatccttt
      541 ggattggcat ttttggaaat tttatggcga taaatccttg acgggcggag ggtttggtct
      601 ttctgcgtgt aaaagagcgt ttggctatcg cttgcccagg caattgttcc ggtagtatta
      661 tcaatcttga ttgggagcaa cttgccggtt tccaagtttt taaagtaaat ggtgaacaat
      721 cttttgccta gggtatcaac cccgaaggca agaatttggt tgttgggcga aatatccaag
      781 ctggcaacct ggcagtaccg ctgaccggca gcaacttcat tctcgtcgag aattacctcc
      841 tcgggggcat ccatgttacc ttttctgcgg cagttaatgg ggtactcttt cccttcctca
      901 aatcgcgtgt agtaaaaata cccgttcata aacgcaggaa ctgtaacatc ctctaccttt
      961 ttgcgcgatt tcaactcgtt gaatagcttt tcctgaagtg gcatggtgga cttcatcatg
     1021 gcctccgtgt aggcattttc agctagcagg tagtccagca ccttttgcga atcgcgatag
     1081 cacaaccaga agtagggatc aatacgggta tcgccacaag taaccatttg gtagggatgc
     1141 acttcagctt tgggtggagc gggtgccagg gagcaactat tcagtaaaac caatcccaac
     1201 aggtagaact ttacgttcca taagcgagct gccataattt tcgataaata aaaccaaaat
     1261 tatgattttg gccggttacc tgcaaatcta ctccacattt attcgtaaca ttttaacgta
     1321 caatacgttg cagatattgt aaagaaaact atccattcag atggtcagct cctactggtt
     1381 acttctcctt atatgcactt acctcaatat ttaaaattcc cctaattttg agcatctatt
     1441 gtgttgaaat ccatgacaaa gcttatttca gccattactt cggatggctc gagcacgatc
     1501 tactccgaac ttcatggcgc atactaccat tcacttaacg
//
LOCUS       DAOOQX010000538         1540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_106919_length_1540_cov_3.330640, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000538 DAOOQX010000000
VERSION     DAOOQX010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106919_length_1540_cov_3.330640"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..909)
                     /locus_tag="PLX53_08175"
     CDS             complement(214..909)
                     /locus_tag="PLX53_08175"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOY73094.1"
                     /translation="MDNLVEQLEVKFLQKGNPAVALQQKKYMRNQFEFFGIQSADRRE
                     IQKLLFTPEHLPAKNELGSTINRLWLKPQREFQYFAQEMAYKFHKQLELADISLYENM
                     VTTKSWWDTVDYIASRLMGEYFASFPEQRKPFIQKWVTSDNLWLKRSALIFQLNYKQQ
                     TDEVLLAATIHSLLPSSEFFICKAIGWVLRNYSKVNPEWVLQFVAHTPLPPLSKKEAT
                     RLIVQPPLRHKQV"
     gene            complement(1046..>1540)
                     /locus_tag="PLX53_08180"
     CDS             complement(1046..>1540)
                     /locus_tag="PLX53_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOY73095.1"
                     /translation="QVASNFTTLRMINDYQNRFYNKLYFRSSELIENDFEVAKKISSW
                     KRRITRNWDEIEIIQVKQFDVSREAIIMGKEYTAEVILDMGSLGPDEIGVEMVLADLI
                     EGGEVKVKRVHEFQVQAVEGSRVTYRLNLIPVEPGAFECGIRIYPKNPMLPHRMDFPL
                     VRWA"
BASE COUNT          391 a          316 c          370 g          463 t
ORIGIN      
        1 ccttcatctg ggcctcagac aaaacgggag tagcaatgaa aacggtttcg taatggttca
       61 ccataaaata tgattttagt taatagggtg caaaattata gctaattttt aaatcttcca
      121 aatacacaga gggtagaatt ctcaagaata aatagcatcg gcttaaggtg tttgcggttt
      181 gcaccctcat gttcagggaa aacttgttga agcctaaacc tgtttatggc gcagtggagg
      241 ttggacaatt aaccttgtag cttctttttt gctgagggga ggcaagggtg tgtgggctac
      301 aaattggaga acccattcag ggttgacttt gctataattt ctaagcaccc atcctatggc
      361 cttacatata aaaaattcac tgctaggcag caaggaatga attgtggcgg cgagaagtac
      421 ttcgtcggtt tgctgcttgt agttaagctg gaagattagt gccgagcgtt taagccaaag
      481 gttgtccgag gtaacccatt tttgaatgaa cggtttccgt tgttcaggaa atgaggcaaa
      541 gtactcgccc atgagccgtg aggcaatgta gtcaaccgta tcccaccacg atttggtggt
      601 taccatgttc tcgtataggc ttatatcggc tagctccagt tgtttatgga atttgtaggc
      661 catttcttgg gcaaagtatt gaaattcccg ttggggttta agccagagcc tgttgatggt
      721 tgagccgagt tcgtttttag caggcaggtg ttctggtgta aacaatagct tttgaatctc
      781 tcgtcggtca gccgactgaa tgccgaaaaa ctcaaactga tttcgcatgt attttttttg
      841 ttgaagcgcc accgctggat tgcccttttg taggaatttt acttccagct gctctacgag
      901 gttgtccatt tgcttggttg tagtttttcg tattatattt aagctactgc tacaggatta
      961 ttatcaaggt tcggcccaat caaagcaaag cttatccgca caacgtggat aaacaagtat
     1021 ggggtgctga actgcatggt ttttgctatg cccagcgtac caacgggaaa tccatgcgat
     1081 ggggaagcat ggggttttta ggatatatgc gtattccgca ttcaaaagca cccggctcta
     1141 caggtatgag gttgagcctg taggtaaccc tggagccttc aactgcttga acttgaaatt
     1201 catggacccg tttaaccttt acttcgcctc cttcaattag gtcggccagc accatctcaa
     1261 ccccgatttc gtcgggacca agggaaccca tgtccaggat aacctcggcg gtgtactctt
     1321 tacccatgat aatggcctcg cgcgaaacat caaactgttt gacctgaatg atttcaattt
     1381 catcccagtt acgggtaatc cgtcgtttcc atgacgaaat ttttttggct acctcaaagt
     1441 cgttttctat aagctcggag gaacggaagt agagcttatt gtaaaacctg ttctggtagt
     1501 cgtttatcat cctaagggtg gtgaagttag atgctacctg
//
LOCUS       DAOOQX010000539         1539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_107053_length_1539_cov_2.687332, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000539 DAOOQX010000000
VERSION     DAOOQX010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107053_length_1539_cov_2.687332"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1539
                     /gene="feoB"
                     /locus_tag="PLX53_08185"
     CDS             <1..>1539
                     /gene="feoB"
                     /locus_tag="PLX53_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOY73096.1"
                     /translation="GFVKGKRVDVVKNAPLNDPIEYSIMGYQVSLRRSEASLIEVVTP
                     DEAKKILAENHTEPLTTLTEPELVQTGLKVMGKTINIALVGNPNSGKTTIFNFLSGSR
                     EHVGNYSGVTVDSKIAHFQHKGYTFNITDLPGTYSLTAYTPEEVYVRNYIHENHPDLI
                     INVVDASNLERNLYLTTQLIDMDVRIVVALNMYDELLDKRDRFDYEMLGKMLGIPFVP
                     TVGSRAKGFDRLKDTIINVFSEKDKTSRHIHINYGAVIEESIAAIQHEVRKNETLTVN
                     YSSRYLSIKLLEKDRLTDRMLKDFPNYAEIRKIARQEVERLEALFHEDSETLITDAKY
                     GFIAGALKETYHESSIKKRQTTEIIDTFITHKLFGFPIFIFLIWLTFFTTFTLGEYPM
                     NWIEAGVAWISRSINGLMPNGPLKDMLVDGIISGVGSVIVFLPNILILFFFISLLEDT
                     GYMARAAFIMDKLMHKIGLHGKSFIPLVMGFGCNVPAIMASRTIENKNNRLVTMLITP
                     FMSC"
BASE COUNT          458 a          350 c          340 g          391 t
ORIGIN      
        1 tgggttttgt gaaaggtaaa agggtggatg tggtgaagaa tgccccattg aatgacccca
       61 ttgagtactc cattatgggc taccaggttt cgcttcgcag aagcgaggct tccctcatag
      121 aagttgtaac ccccgatgaa gcgaaaaaaa tattggccga aaaccacacc gagcctttaa
      181 ctaccctaac agaaccggaa ctggtgcaaa ccggcttgaa ggtgatggga aaaaccatca
      241 atatagccct tgttgggaat cccaattccg gtaaaaccac catattcaac ttcctttccg
      301 gttcaagaga gcacgtggga aactacagcg gcgttacggt tgattccaaa atagcccatt
      361 ttcagcacaa ggggtacacc ttcaacatta ccgacctacc gggaacctac tcgctaacag
      421 cttacacccc tgaggaggtt tacgttagaa actacatcca cgaaaaccat cccgacctta
      481 ttatcaacgt agttgatgcc tcgaacctcg agcgaaacct ttacctcacc acccagctca
      541 tcgacatgga tgtgaggatt gtggttgccc taaacatgta tgatgagctg ctggataaaa
      601 gggacaggtt tgactacgaa atgctgggta aaatgctagg cattccgttt gttcccaccg
      661 ttggttccag agctaaaggt ttcgacaggc tgaaggacac cattataaac gtattctcgg
      721 aaaaggataa aacctcgcgt catatccaca taaactatgg agctgtaatt gaggaatcaa
      781 tagcagcaat ccagcatgag gttagaaaaa atgagactct tacggttaac tactccagca
      841 ggtacctatc aataaagctt ttggaaaaag ataggctgac tgaccgcatg ctgaaggatt
      901 ttccaaatta cgctgaaata cgcaaaattg cccggcaaga ggtagaaagg ctagaagcgc
      961 tgttccatga ggattctgaa accctgatta ctgatgccaa atacggattc attgcaggag
     1021 ccctaaagga aacctaccac gaaagcagca taaaaaaaag gcaaacaact gaaattattg
     1081 acacgtttat cacccataag ctcttcgggt tcccaatttt cattttttta atctggctca
     1141 cctttttcac cacctttact ttaggtgagt accccatgaa ctggatagaa gcgggggttg
     1201 catggattag tcgttctata aatggcctaa tgcccaatgg cccattaaag gacatgttag
     1261 tagatggaat tattagcggt gttggtagtg taatagtctt ccttccaaat atcctcattc
     1321 ttttcttctt tatatccctt ctggaggata ccgggtacat ggccagggct gccttcatca
     1381 tggataagtt gatgcacaag atagggctgc atggcaaatc gttcatcccg ctggttatgg
     1441 ggtttggatg taacgtgccg gctataatgg cctcacgaac cattgaaaat aagaataacc
     1501 ggctggttac catgcttata actccattca tgagctgta
//
LOCUS       DAOOQX010000540         1531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_107961_length_1531_cov_3.085366, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000540 DAOOQX010000000
VERSION     DAOOQX010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107961_length_1531_cov_3.085366"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1403)
                     /locus_tag="PLX53_08190"
     CDS             complement(120..1403)
                     /locus_tag="PLX53_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HOY73097.1"
                     /translation="MDSITAVNRKALPPIFIIGALFFVFGFVTWLNGILIPYFKISCE
                     LTDFEATLVAFAFYISYTIMALPSSWILKRTGFHKGMTLGLLLMSVGTLLFVPAALSR
                     AYWMFLSGLFVMGAGLAILQTAANPYIAILGPRESAARRISIMGICNKLAGAVAPLIL
                     AYYILQDGDTFVQSLSGLDEVARMAALDSLAARVIGPYLVMTGVLVVLAIFVRYSPLP
                     EVEAEPEEVEADVAELAQKKNILQFPHLVVGAIALFFYVGVEVIAGDTIIRYGISQGI
                     AISTAKAFTSYTLFAMIVGYLLGIALMPKIISQRTALTISASLGLLFSLGVILSRGST
                     SIVFLALLGFANALIWPTIWPLAIHNLGRFLKTGSAILIMAIAGGAIIPLIWGKLSDI
                     YSQTPQVAYMVLIPSYLIILYYSVWGYKLRSWRRR"
BASE COUNT          455 a          326 c          384 g          366 t
ORIGIN      
        1 acgaaacttt atggccagaa tatcattgaa gattaaccgg gcaatgtagg tcaggcgtga
       61 ggaattattt ttggtatata taagtagttc cactacaggt agttacaggt gatatggttt
      121 taccttctac gccatgatcg tagtttatac ccccaaacgg agtagtagag gatgataagg
      181 taagatggaa taagtaccat gtaggcaact tgaggggttt gggaatatat atcgctaagt
      241 ttaccccaga taagcgggat gatggctccg ccggcaatgg ccatgataag aattgccgag
      301 ccggttttaa ggaatcggcc caggttgtga atggcaagcg gccaaattgt tggccaaatc
      361 agggcgttgg caaagccaag cagggccaga aacacgattg atgtactgcc ccttgacaaa
      421 atcaccccaa ggctaaaaag caaccccagc gatgctgaga tggtaagggc tgtccgctgg
      481 gagataattt ttggcatgag cgctataccc agcaggtagc ctacgatcat ggcgaataga
      541 gtgtaggagg taaatgcttt tgcggttgat attgcaatac cctgcgaaat tccgtagcga
      601 ataatagtat cgccggctat tacttcaacg cctacgtaga agaatagtgc tatagcgcca
      661 acaaccagat ggggaaactg caggatgttt ttcttttggg ccaattcggc aacgtcggct
      721 tccacttctt cgggttcagc ttctacctcg gggagagggg agtaccgaac gaatatggcc
      781 agaacaacta gcactccggt cataaccagg taggggccaa taacccttgc cgcaaggcta
      841 tcaagtgctg ccatacgggc aacttcgtcg agcccgctaa gcgactgcac aaatgtgtca
      901 ccatcctgaa gaatgtagta ggccaggata agtggggcaa ctgcgccggc cagcttgttg
      961 catataccca tgatgcttat ccgtcgggct gcactttcac gaggtcccag tatggctatg
     1021 tagggatttg ctgcagtttg cagaatggcc aacccggcgc ccattacaaa aagcccggaa
     1081 aggaacatcc agtaagctct acttaacgct gctggaacaa aaagtaacgt acctaccgac
     1141 attaacagca gccctaaggt cattccctta tgaaaacccg ttcgcttgag aatccatgag
     1201 gagggaaggg ccattatggt gtaggatatg tagaaggcaa aagctaccag cgtagcctca
     1261 aaatcagtca gctcacacga aattttgaag tatgggatta atattccgtt aagccatgtt
     1321 acaaatccaa aaacaaagaa tagcgcacca atgataaata tgggtggtaa agcctttcgg
     1381 ttaacagctg ttattgagtc catagaagtt cggttaggtt tctcaaaaat attaaatggt
     1441 tgaaatttta agcaatagcc ttcgaaataa tttagggaat taatgcgatt aataaccgga
     1501 ataacggcct agtctttaag tgagtaaccc a
//
LOCUS       DAOOQX010000541         1524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_108807_length_1524_cov_3.210347, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000541 DAOOQX010000000
VERSION     DAOOQX010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108807_length_1524_cov_3.210347"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1150)
                     /locus_tag="PLX53_08195"
     CDS             complement(53..1150)
                     /locus_tag="PLX53_08195"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOY73098.1"
                     /translation="MRFSSMMVGLFLGLTMLSCSNKMATVSPVRSAPPVKDYSFIYTL
                     PRTVILIKVDACRSVFYPGPYAAYARKYLGISDVPTLKSEQWTITDITIKGISESDPS
                     MLFAVFTDGHVPDALVQMTEAGLVIPENRAVIVSMMSKDLRRIENQAIPFSDLSVEPF
                     IGEEKNILYTKVQRDSSFVRVPIQKSMIVEKNADEKAGEAADLIFNLRKRRLEFLTTD
                     VDQPLEGNAVHVILDEIYRLENEYLSLFLGKTAVDTISKTYLFTPSGENNEKAIAFRF
                     SPTKGLLAQNDLSGSPMLVEVEPEPVPDSSEPLLATIGRLAEKQKLAQIFYRIPVVST
                     VKISDGKSEIASRRIPIYQLGPLVKIPAEFY"
     gene            complement(1170..>1524)
                     /locus_tag="PLX53_08200"
     CDS             complement(1170..>1524)
                     /locus_tag="PLX53_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOY73099.1"
                     /translation="CGWKRLERAREVAQRYQHVIILKGAYSQVVLPDGRVFFNSTGNP
                     GMATGGSGDVLSGIVVGFLAQGFSIADAALLATFVHGLAGDIAASDLGENALVATDMI
                     SYISNALLEIEISNL"
BASE COUNT          459 a          343 c          336 g          386 t
ORIGIN      
        1 tcaaagtttg taagcaagct aacgagcggt gaataagttt agcttacctg gattaataaa
       61 actctgcagg aattttaacc aaaggtccta gctggtaaat aggtatgcgg cgggaagcaa
      121 tttccgactt accgtcggaa atttttacgg ttgagacaac cggaatacgg taaaaaattt
      181 gtgccagctt ttgcttttcg gcaagacgac cgatggttgc caaaagaggt tcggaggaat
      241 cgggaaccgg ttcgggttca acctcaacaa gcatgggact acccgacaag tcattttggg
      301 caagcagccc ttttgtaggg gaaaatcgaa aggcgatagc cttttcatta ttttctcctg
      361 agggggtaaa aagataggtt ttggaaatcg tatcaacagc tgttttacct aagaacaacg
      421 aaaggtattc gttttcgagc ctataaattt catcgagaat tacatgaaca gcattgccct
      481 ccaagggctg atcaacatca gtagtaagga actccagcct tcgcttgcgt aggttgaaaa
      541 tgaggtcggc tgcctcgcca gccttttcat cagcattctt ctcaacaatc atgctttttt
      601 gaataggaac ccttacaaag ctactatcgc gctgcacttt ggtgtataaa atatttttct
      661 cctccccaat aaagggttca accgataggt cggagaaagg tatagcctga ttttctattc
      721 tccttaaatc tttactcatc atggacacaa taacagccct attttcggga attaccaagc
      781 cagcctcagt catctgaacc agagcatcag gcacatgacc atcggtgaat acggcaaaaa
      841 gcatggatgg atcggactca cttatacctt taatagtgat atcggtaatg gtccactgct
      901 cgctttttag agttggcaca tcggaaattc ctaagtattt cctggcatac gcagcatagg
      961 gaccaggata gaacacgcta cgacaggcat caaccttaat caaaataaca gttcggggta
     1021 aggtgtatat gaagctgtaa tcctttactg gtggagcaga cctaacaggc gaaacggttg
     1081 ccattttgtt gctgcacgag agcatagtca ggcctaagaa aagaccaacc atcattgatg
     1141 aaaagcgcat gatcatagtt tggctaaatt tacagattcg agatttcaat ttcaagtagt
     1201 gcatttgaaa tgtagctaat catatcagtt gcaacaaggg cattctcacc taggtcgctg
     1261 gcagcgatat cgccggccaa accatgtaca aaggtagcaa gtagcgccgc atcggctata
     1321 gaaaaacctt gggctaaaaa acctacaacg atacccgaga gaacatcacc acttccgcct
     1381 gtagccatgc ccggatttcc ggtagaatta aaaaagacac gcccatcggg caaaactacc
     1441 tgggagtaag caccttttag aataattacg tgctgatagc gctgagcaac ctccctggca
     1501 cgctccaaac gtttccagcc acaa
//
LOCUS       DAOOQX010000542         1524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_108852_length_1524_cov_2.411164, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000542 DAOOQX010000000
VERSION     DAOOQX010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108852_length_1524_cov_2.411164"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLX53_08205"
     CDS             complement(<1..328)
                     /locus_tag="PLX53_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY73100.1"
                     /translation="MITVKNLSKSFDGRMVIDNITATFEKGKTNMVIGQSGSGKTVLL
                     KCIVGLLDVDEGEIYYNDIQFNALSFKKKKEIRQKIGMLFQGSALFDSQTVEENVMFP
                     LNMFTNM"
     gene            complement(325..1068)
                     /locus_tag="PLX53_08210"
     CDS             complement(325..1068)
                     /locus_tag="PLX53_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOY73101.1"
                     /translation="MVRIFGLIGEYFLLLKKVFSKPEKGRIYFKQTIKEMDKLGIKSI
                     GIVTIISVFMGAVITLQTAYNMENPFIPRYMIGLGTRDSMFLEFSSTIIGLILAGKVG
                     SSIASELGTMRVTEQIDALEIMGVNSASYLILPKIIATLIFNPFLCMLSMVVGIAGGW
                     MVGEFSGVISTQAYIYGIRYAFIPFYITYSLIKTVVFAFLITTISAFYGYRVQGGSLE
                     VGQASTNAVVVSSVFILLFNLILTQLLLS"
     gene            1416..>1524
                     /locus_tag="PLX53_08215"
     CDS             1416..>1524
                     /locus_tag="PLX53_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009293221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOY73102.1"
                     /translation="MNNKNFYCIIMAGGIGSRFWPLSKPACPKQFIDILG"
BASE COUNT          430 a          338 c          301 g          455 t
ORIGIN      
        1 ccatgttggt gaacatgtta agaggaaaca tcacattttc ctcaacggtt tgcgaatcga
       61 atagcgctga cccctgaaaa agcatgccta ttttttgtcg tatttctttt ttctttttga
      121 acgaaagggc gttaaactga atgtcgttgt agtaaatttc tccctcatcc acatccagca
      181 ggcctactat gcacttcagc aggacggttt ttcccgaacc gctttggccg attaccatgt
      241 tggtttttcc cttttcaaac gtggccgtta tattgtcaat caccattcgg ccatcaaacg
      301 attttgacag atttttaacc gtaatcacga cagtagcagt tgggttagaa ttaggttaaa
      361 caggagaata aagacgctgc tgacgacaac cgcattggta cttgcctggc ccacctcgag
      421 cgacccgccc tgaacgcggt aaccataaaa tgcagaaata gtggtgatta ggaaggcaaa
      481 taccacggtt tttattagcg agtaggtgat gtaaaagggg ataaacgcat accttatacc
      541 gtaaatgtag gcttgtgtag atattactcc tgaaaattct ccaaccatcc atcctccggc
      601 tatcccgacc accatgctta gcatgcaaag aaaagggtta aaaataaggg tggcaataat
      661 tttaggaaga atcaggtagc tggcggagtt aacacccatg atttcgaggg catcaatttg
      721 ctcggttacc ctcatggtgc ctagttcaga tgctatgctg gaacccacct tgccggcaag
      781 tattaggcca atgattgttg aagagaactc cagaaacatg ctgtcgcgcg tgcccaaccc
      841 aatcatgtag cggggaataa aagggttttc catgttatat gcggtttgta gggttattac
      901 cgctcccata aaaaccgata ttatggtaac aatcccgatg gatttaatac ccagcttatc
      961 catctcctta atggtctgct taaagtatat ccttcccttc tcaggttttg aaaaaacctt
     1021 tttcagcaga agaaaatact ccccgattaa tccgaatatt ctaaccatct tcgacttttt
     1081 tacttcttaa cgttcccaga aagctagctt cctgtaataa aatgccagca accgaatatt
     1141 aacctgcagc aaaattagtc aatattaaag tggtactaaa attaatcata ctcatttaaa
     1201 cctgctctat tctccctgcc ctttgagtgg gataagctct gattttaacg ctgccaggac
     1261 aatccgcagc tggttttatg tcaacgtttt tttgttcggc ttgttctaaa agcctatttt
     1321 tactggctcc tgttagcttt tcaactcaat atcggatgtt gaaagtttcc atctcaagct
     1381 aaaaatccgt acgtttgcat tttaatgcat gagcaatgaa caataagaac ttctactgta
     1441 taattatggc cgggggaatt ggaagccgtt tttggccttt aagcaaacct gcttgtccaa
     1501 aacagtttat tgatatactc ggcg
//
LOCUS       DAOOQX010000543         1522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_109096_length_1522_cov_2.199727, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000543 DAOOQX010000000
VERSION     DAOOQX010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109096_length_1522_cov_2.199727"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="PLX53_08220"
     CDS             <1..1290
                     /locus_tag="PLX53_08220"
                     /inference="COORDINATES: protein motif:HMM:NF027450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family zinc-binding domain-containing
                     protein"
                     /protein_id="HOY73103.1"
                     /translation="TYVHIYKKNDPAKFAEYRLSDGLYDRIQENMNLPTVDTFGIVSS
                     PVFGPASAWKNAEWNGSALDDGKGDRVRVKVIGVQANGTETGLFDVPVTQSSVDLSSV
                     SPLQYPEIKMALYNTDSIRGTPWNLNYWRVYFTPVPEGALAANLLLQDKDTLQQGETY
                     DFNLMDFCSAYKLSSVRAYSSLKILEREGYLELTDELDNPSRIMFLAGKQDLYKFQVA
                     HAELDGFIKAILRNFTGVFSQYVRIDEVYLARLAGITVDKVIENLKTLGRYKIISYIP
                     KKRTPLLVLVEERLDDKNLRIEPSSYRLLRSRFEDRVKAMLSYANNQSKCRSQMLSEY
                     FGQKDSYRCGKCDVCTSRNELDMSKYEFDMILDGLKRRLQAGPCSEEELIDAIKRNPD
                     RVARVIKYLMDTGKIFHNEKGMLAWHHRKEGGFEVEE"
BASE COUNT          444 a          321 c          367 g          390 t
ORIGIN      
        1 acctacgtac atatttacaa aaagaatgac ccggccaaat ttgccgaata ccgcctgagt
       61 gatgggctgt acgaccgcat acaggaaaat atgaacctgc ctaccgttga tacctttggt
      121 attgtttcgt caccggtatt tggccctgca agtgcctgga aaaatgccga atggaatggc
      181 agcgcgctag atgatggtaa gggcgaccgg gttagggtaa aagttatcgg ggtgcaggcc
      241 aatggaaccg aaaccggttt atttgatgtg ccggttaccc aaagttcagt tgatttatct
      301 tcggttagcc ccttgcaata ccccgaaata aaaatggccc tatacaatac cgattcaata
      361 agaggaaccc catggaacct gaactattgg agggtatatt tcacgccggt accagagggc
      421 gcattagcag ccaacctgct tctccaggat aaggataccc tgcaacaggg cgaaacctac
      481 gatttcaacc ttatggactt ttgctcagcc tataagctta gctccgttag ggcttatagc
      541 tcgctaaaaa ttcttgagcg tgaagggtac cttgaactta ccgacgagct tgataacccc
      601 agccgcatca tgtttcttgc cggtaagcag gatctctaca agttccaggt tgctcatgca
      661 gaactggatg ggtttattaa ggcgatactc cgaaatttta ccggagtttt ctcccaatac
      721 gtccgaattg acgaggttta ccttgcccgg ctggcgggta ttactgttga taaagtgatc
      781 gaaaacctta aaacccttgg caggtataaa atcataagct acattcccaa gaaaagaact
      841 ccgctgctgg tactcgttga ggagaggctt gacgataaaa atttgcgcat tgaaccaagc
      901 tcatacaggt tgcttaggtc acgctttgag gatagggtaa aggctatgct ttcttatgca
      961 aataaccaat ccaagtgcag aagccaaatg cttagcgaat actttggcca gaaggactcc
     1021 taccgctgtg ggaaatgcga tgtatgcaca tcgcgcaacg agttggacat gagcaagtat
     1081 gagtttgata tgattctgga tgggcttaaa aggcgcttgc aagctggccc atgtagcgag
     1141 gaggagctta tagatgcaat taaacgtaat cctgatagag tagccagggt gatcaagtac
     1201 cttatggata ccggaaaaat tttccataac gaaaagggta tgctggcatg gcaccatcga
     1261 aaggaaggcg gattcgaggt agaggagtga gcacctaagc gactattggc ttcaacttaa
     1321 agttaagaag aatcaattag gcgtgaaaaa cctagttttg acacgaaaag tgtttttagt
     1381 aagtatgtta aaagttaatt ggataaagaa tacttttaag ccatcaacgg ttgaacaagt
     1441 tccaccggga ggcggctttc aaattatact ttattcttta tcctttaccc tctttaatct
     1501 ggaatccgcc agtagtcgga ca
//
LOCUS       DAOOQX010000544         1521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_109183_length_1521_cov_2.554570, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000544 DAOOQX010000000
VERSION     DAOOQX010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109183_length_1521_cov_2.554570"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="PLX53_08225"
     CDS             <1..1064
                     /locus_tag="PLX53_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOY73104.1"
                     /translation="LIELPFNSIVRNYINVYTQKKKSQVEVMLGLTDYYFPIFEEILD
                     KYQLPQELRFLPVIESALNPKAVSRAGATGLWQFMYGTGRRYNLTVNSYVDERRDPIA
                     STHAAARFLRDLYAIYGDWTLVIAAYNCGPGNVNKAIRRSGGKRNYWDIYYYLPRETR
                     GYVPAFIAASYTYYFSHEHNLVPQPINIPPTTDTIVINDMLHLQQVSEVLDIPLDMLR
                     DLNPQYKLDVIPGKFKPYALRLPLDGVGPFIDREKDIYAYKDSLFFNPRVTISPNKYT
                     SSSQYDIPSGSIRYVYRVKEGDVLGIIAEKYGVSVGQLRSWNNLRGNIIRIGQRLVIY
                     IPERTANKMGISGDKSQTS"
     gene            1162..>1521
                     /locus_tag="PLX53_08230"
     CDS             1162..>1521
                     /locus_tag="PLX53_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73105.1"
                     /translation="MAHNRNKAALSIALSFLLFVTTACYSGKKTADYSTLTNNAFAFV
                     EYYQTTDVQVMAGEYHGGRRIDSPTYTFNPEDRTLNIYRYPDGLQPDSAIVVLGVGKI
                     LKNAAGSGVSSFLVGANQ"
BASE COUNT          431 a          386 c          330 g          374 t
ORIGIN      
        1 cgcttattga gctgccattc aacagcatag tcaggaacta catcaacgtg tacacccaga
       61 agaagaaaag ccaggttgag gtgatgctag gactaaccga ctactacttc cccatatttg
      121 aggagatact cgataagtac cagctgccac aggagctaag gtttttaccg gtaatagaat
      181 cggcgttaaa ccccaaagct gtttctcgtg ccggcgccac aggattgtgg cagttcatgt
      241 atggaacagg tcgaaggtat aacctaaccg ttaactctta cgttgacgag cgtcgtgacc
      301 caattgcctc aacgcacgct gcagcacgat ttctgagaga tctctacgcc atttatggag
      361 actggacatt ggtgattgcc gcctacaact gtggtccagg caacgtgaat aaggcaattc
      421 ggcgctcggg cggcaagcgc aactactggg acatatacta ctacctacca agagaaacac
      481 gcggctacgt cccggccttt atagccgcca gctacaccta ctattttagc cacgagcata
      541 acctggttcc ccagcctatt aacatacccc ctactaccga taccattgtg attaacgaca
      601 tgcttcacct ccaacaggtt tccgaggtgc tggatatccc tttggatatg ctccgcgacc
      661 ttaaccccca gtacaagctc gacgttattc ccggcaagtt caagccttac gcccttaggc
      721 tgccgctcga cggagtagga ccttttattg acagagaaaa agatatctat gcctataagg
      781 acagcctgtt ttttaaccca cgggtaacca ttagcccaaa taaatataca tcatcctccc
      841 agtacgatat accatcgggc tcaatccggt atgtttacag ggtaaaagaa ggcgatgtat
      901 tagggataat tgccgaaaaa tacggtgtat ccgtgggtca gctaaggtca tggaacaacc
      961 ttaggggcaa catcattcgc attggacagc gcctggttat ctacattcct gaacgtactg
     1021 caaataaaat ggggattagt ggcgacaaga gccaaacctc atagagtttt cactctagca
     1081 tgaattgaca cattcatgga aagtatgacc ttaccagcat aaggcagatg ggttttgcaa
     1141 aacacctaca agcaattcgc tatggcacac aatcgtaata aagcagcact ttctattgct
     1201 ctttcttttt tattatttgt aactaccgca tgctattccg gtaaaaaaac agccgattat
     1261 tcaaccttaa ccaacaatgc ttttgccttt gttgagtact accaaacaac agatgttcag
     1321 gtaatggcag gtgagtatca tggcggcagg cgaattgatt cgccaaccta cacattcaac
     1381 cccgaggaca gaacacttaa catataccgt taccccgacg ggctgcaacc cgattccgct
     1441 atagttgttc ttggggtggg caaaatctta aagaatgctg ccggaagcgg ggtttcctca
     1501 ttcctcgtag gggctaacca a
//
LOCUS       DAOOQX010000545         1520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_109259_length_1520_cov_3.834130, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000545 DAOOQX010000000
VERSION     DAOOQX010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109259_length_1520_cov_3.834130"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="PLX53_08235"
     CDS             <1..1363
                     /locus_tag="PLX53_08235"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOY73106.1"
                     /translation="ILTAAISSPTANQQCLVSYKSSDFEPSSTTTRTDTLFKENFNEN
                     NLDKFDTVSVKGAQKWAASAYSGNYFAKISGYSGGVNNENEDWLIIPAIDLSGVQSAT
                     LNFTTAWKYDGNDLLLKYSTNYSGSGDPNAASWTNLSFDLGTGNFVFCPSGNVPLPKA
                     KIYIAFVYTSTTSASRTWEVDDVVITAKVLAKGKSVTADLFKNYNDYYRFTGSKWEKS
                     KGVLCVQPFEYSLMGLTGNAFTNTNPASRYIPTYLSQKYPYAQDGDTCIMVYNYPNAN
                     TKVAGKYQNSAGSWMVYNGIVQQSGQFIHTGEHWIFDPTVHLTPSASDLQLLVDYVYL
                     TYGRQYGSSYGNDEFYFGASAYYKNFDLRLSTRSQYNVPGFAELATDEEKIALTWQRL
                     QEGLTILLKLKYANDAVLEVDGIPVYYWVTFNTYENDLAKKTYVGIFKCTQPAPDPIF
                     ERD"
BASE COUNT          447 a          327 c          345 g          401 t
ORIGIN      
        1 gattctgaca gctgcaattt cgtcgcccac agctaaccag caatgcctgg tttcctataa
       61 atcgtctgat tttgagccat ctagtacaac aacacgcact gatacccttt ttaaggaaaa
      121 ttttaacgag aacaacctgg ataagtttga cactgttagc gtcaaagggg cgcaaaagtg
      181 ggctgcttca gcatatagcg ggaactactt tgccaaaata tctggttact ccggtggagt
      241 aaacaacgaa aatgaggatt ggcttatcat tccggcaatt gacctctcgg gcgtgcaaag
      301 cgcaacgctc aactttacta cagcatggaa gtacgatgga aacgatttac tgctgaagta
      361 ttcgaccaac tacagcggta gcggcgatcc aaatgcggcc tcatggacaa acctgagttt
      421 tgacctggga acaggcaact ttgtattttg tccttcggga aatgttcccc ttcctaaagc
      481 caaaatttac attgcatttg tgtacacttc aaccacatcg gcaagtagaa catgggaggt
      541 agatgatgtg gttattacag ccaaggtgtt ggctaaaggg aaaagtgtta cagccgacct
      601 tttcaaaaac tacaatgatt actataggtt tacaggctcc aagtgggaaa aatcaaaagg
      661 ggtgctttgc gttcaaccat tcgaatacag cctaatgggg cttacaggga acgccttcac
      721 aaatactaat cccgctagcc ggtatattcc aacctacctt tcccaaaaat acccctatgc
      781 acaggacggg gatacctgca taatggttta caactatccg aacgccaata ccaaagtggc
      841 cggtaaatac cagaatagtg cagggagttg gatggtgtac aatggtattg tgcagcagtc
      901 gggacagttc atccacactg gagagcattg gatttttgat ccaaccgtac atttgactcc
      961 tagtgcctcc gacctgcagc tgttagttga ttatgtttac cttacttatg gacgccagta
     1021 cggaagcagc tatggtaacg acgagtttta ctttggagcc agcgcatact acaaaaattt
     1081 tgaccttagg ctttctacaa gaagccagta caacgtgccg ggatttgccg aacttgccac
     1141 cgatgaggaa aaaattgcgt taacgtggca aaggctccag gaaggtctga ctattttgct
     1201 aaagttgaag tacgcaaacg atgcagtatt agaggttgat ggaattcctg tttactactg
     1261 ggttacattc aacacctacg aaaatgactt ggccaaaaaa acctatgtgg gtatttttaa
     1321 atgtacccaa cccgcacctg atccaatttt tgaacgcgat taagatgctg aggatctggc
     1381 ggtaagtgat ggcaagcttt tacaatcagc tgtaagttgg aatagggaaa agtagtttta
     1441 gctaatagct agtagtaata aagcgcccga attaacatcg ggcgctttta ttgtatccat
     1501 ggccatttag acgtttcttt
//
LOCUS       DAOOQX010000546         1518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_109485_length_1518_cov_2.961722, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000546 DAOOQX010000000
VERSION     DAOOQX010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109485_length_1518_cov_2.961722"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /gene="rplO"
                     /locus_tag="PLX53_08240"
     CDS             complement(<1..380)
                     /gene="rplO"
                     /locus_tag="PLX53_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOY73107.1"
                     /translation="MNLNNLKPAKGSTRNRKRVGRGTGSGHGRTSGRGQKGAQSRSGH
                     SYKVGFEGGQMPLQRRMPKYGFKNPFRIEYQAINIGTLQALAEKNGYSIIDVDTLIEA
                     GLVTRNERIKILAKGELTAKLEVKA"
     gene            complement(412..585)
                     /gene="rpmD"
                     /locus_tag="PLX53_08245"
     CDS             complement(412..585)
                     /gene="rpmD"
                     /locus_tag="PLX53_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOY73108.1"
                     /translation="MAKVKITQIKSVIGHPERQRRTLRSLGIKRMHQTVEVEGTPQVL
                     GMIAKVRHLIRVE"
     gene            complement(598..1119)
                     /gene="rpsE"
                     /locus_tag="PLX53_08250"
     CDS             complement(598..1119)
                     /gene="rpsE"
                     /locus_tag="PLX53_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOY73109.1"
                     /translation="MSANTNIHRVKASDLELKDRLVSIQRVTKVTKGGRTFSFSAIVV
                     VGNEDGVVGYGLGKASEVTAAIAKGIEDAKKNLVKIPVFKGTIPHEQEAKYGGAKVII
                     RPASQGTGVKAGGAMRAVLESVGITDVLAKSKGSSNPHNLVKATVMALLELRDARTIA
                     EQRGISLSKVFNG"
     gene            complement(1125..1484)
                     /gene="rplR"
                     /locus_tag="PLX53_08255"
     CDS             complement(1125..1484)
                     /gene="rplR"
                     /locus_tag="PLX53_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOY73110.1"
                     /translation="MALSKEERRLRIRRRIRKKISGTAEKPRLTVFRSNTNIYVQFVD
                     DVTGRTLASASSMVKDIAGKPKITKTEQAKLVGQLAAKNALEKGITTVVFDRSGYLYH
                     GRVKALADAARESGLKF"
BASE COUNT          348 a          401 c          308 g          461 t
ORIGIN      
        1 gctttaacct ctagctttgc agtaagttcg cccttagcaa gaattttgat acgctcgtta
       61 cgggtaacca accctgcttc aatcagggta tccacgtcaa taattgaata tccatttttc
      121 tcagccagcg cctgtagtgt cccgatgtta atggcctggt attcaatcct gaatggattt
      181 ttaaaaccat atttaggcat tcgtcgctgc aggggcatct ggccaccctc gaaaccaacc
      241 ttgtaggaat ggcccgaacg cgattgtgct cccttttgac cgcgtccgct ggttctgccg
      301 tgcccggatc ccgttccacg tccaaccctt ttccgattgc gggtagatcc cttggcaggt
      361 tttaagttat ttagattcat agttttatcc atttttaagg ttctgtaggt actactccac
      421 ccgtataagg tgtcttacct tggctatcat gcccaatacc tgtggagtac cttctacttc
      481 aacggtttga tgcattcttt taatgcccag cgacctgagg gtacgccgtt ggcgttctgg
      541 gtggccaata acgctcttga tttgggtaat tttaactttt gccatggctt taaaaaacta
      601 accgttaaaa actttgctta atgagatacc ccgttgctcg gcaatagttc tggcatcacg
      661 aagctcgaga agtgccatta ccgtagcttt aaccaagttg tgagggttag atgagccttt
      721 agattttgcc agcacatcgg taattccaac gctctccagc acagcgcgca tggcgcctcc
      781 cgctttaacc cctgttccct gggaggcagg tctgatgata accttggcac caccgtactt
      841 ggcttcctgc tcgtggggaa tagtaccctt gaaaaccggt attttaacca ggtttttctt
      901 ggcatcttca atacccttgg caatggcggc agtaacttca ctggctttac ccaaaccgta
      961 acccacaacg ccatcctcgt tgccaactac tacaattgct gaaaagctga aagtccggcc
     1021 acctttggta accttggtta cacgctgtat gcttaccagc cgatccttta actccaggtc
     1081 gctggctttt accctatgta tgttagtgtt tgctgacatt atcgttagaa tttaagtcct
     1141 gattcccgag ctgcatcggc taatgcctta accctaccat ggtataggta gccactgcgg
     1201 tcaaacacca cggtggtaat tcccttttcc agggcattct ttgcagccag ctgcccaacc
     1261 aatttggcct gttcggtttt ggtaatttta ggtttcccag caatatcctt taccatcgac
     1321 gatgcgctgg ctaaagttcg accggtaacg tcatccacaa actgaacata aatattggta
     1381 ttactcctaa aaaccgttaa tcgtggtttt tcagccgtac ccgaaatttt cttccgaatt
     1441 cgtctcctta tacgaagcct tctttcctct ttacttaaag ccatatctca aaatagatta
     1501 acaggttaca cacttgcc
//
LOCUS       DAOOQX010000547         1517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_109584_length_1517_cov_3.807798, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000547 DAOOQX010000000
VERSION     DAOOQX010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109584_length_1517_cov_3.807798"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1517)
                     /locus_tag="PLX53_08260"
     CDS             complement(<1..>1517)
                     /locus_tag="PLX53_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOY73111.1"
                     /translation="LMLNSKGVYEYAFQDGKGDITLSGVEANNPAERTKAEREFLGKV
                     SKGLYYSPSQLSLMKSIWEIKSAESLKAFPTTGNRKLICILMGFKDKAFTKTQSDFNN
                     LFNQVGYTTGGATGSVKDFYMENSWSQFNLTVDVFGPYTSSQNMSYYGANDASGYDVN
                     PRALVTEAVNAADASANYANYDNDGDGTVDGVYVIYAGYGEEAGASADCIWAHAWSIT
                     PVTKDGKTISKYSCSSELRGTSGTTITAIGVICHEFGHVLGAPDYYDTDYSTGGQYEG
                     TGYWDLMASGSWNNNGVTPAHHNAYTKVKVYGWATATVLSSAATISVTNAEANKSFYQ
                     INSTTTNEYWIMENRQQVGFDANIPGHGLLIYHVHKDIATASSSNNINATYPQKMYPV
                     CASATTNPGSTPSSYGSISSTGCPWPGSSSKTSFTDATTPNMKSWAGANTAKPMTNIA
                     ENTSTKTVTFDFMGGSGGGTAPTATTLAASAISTSSATLNGQVNANNATTTVTFE"
BASE COUNT          326 a          363 c          398 g          430 t
ORIGIN      
        1 attcaaaggt taccgttgta gtggcgttgt tggcattcac ctgtccgttc agggttgcgc
       61 tgctggttga aattgccgag gctgccagcg tggttgctgt gggagccgtt ccgccaccag
      121 aaccgcccat gaagtcaaag gttacggttt tggttgaagt gttttcggca atgttggtca
      181 tgggtttggc agtgttagcg ccggcccacg atttcatgtt gggagtagtg gcatcggtga
      241 atgatgtttt actgctagag ccaggccatg ggcatccggt gctgctaatg ctaccgtatg
      301 agctcggagt gctgccgggg ttggtggttg cactggcaca aacgggatac attttttgtg
      361 gataggttgc gttgatgttg ttgctgctgc ttgccgtggc aatatccttg tgaacatggt
      421 aaattaaaag accatgaccg ggaatgttgg catcaaaacc aacctgctgg cggttctcca
      481 taatccagta ctcgttggta gtggtggagt ttatctggta aaagctcttg ttagcctcgg
      541 cattggtaac cgatatggtg gctgcagagg agagaacggt agcagtagcc cagccgtaaa
      601 ccttaacctt ggtgtaggcg ttatggtggg cgggcgttac cccgttgttg ttccacgatc
      661 cgctggccat taaatcccag tagcctgttc cttcatactg gccaccggtg ctgtagtcgg
      721 tatcatagta atcgggagca cccagcacat gaccaaactc atggcaaatt acacctattg
      781 cagtgattgt tgttcccgaa gttcccctaa gctcggagga gcaggagtac ttgctgatgg
      841 ttttgccatc ctttgtaacc ggagttatgc tccaggcatg agcccaaatg caatctgcgc
      901 tggcacctgc ttcctcgcca tacccggcgt agataacgta aactccatcc accgtgccat
      961 cgccatcatt gtcgtagttg gcatagttgg ccgaggcatc ggcggcattc acggcttcgg
     1021 ttaccaatgc tcttgggtta acatcataac cagaggcatc gtttgcacca tagtacgaca
     1081 tgttctggct ggaggtgtag gggccaaata catcaacggt aaggttaaac tggctccatg
     1141 aattttccat gtagaagtcc ttgacgctcc cagtagcacc gcctgtagtg tagccaacct
     1201 ggttgaacag gttgttgaaa tccgattgtg ttttggtgaa ggctttatcc ttgaatccca
     1261 tcaatataca aatcaacttt ctgttgcctg ttgttgggaa ggccttcaag ctctcagccg
     1321 attttatttc ccaaatactt ttcatcagcg aaagctggct tgggctatag tacaacccct
     1381 tgcttacctt tcctaaaaat tcacgttccg cttttgtcct ttcagcaggg ttatttgcct
     1441 caacgcccga tagcgttata tcgcccttgc catcctgaaa agcatactcg taaactccct
     1501 tgctgttgag cataagg
//
LOCUS       DAOOQX010000548         1504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_111215_length_1504_cov_2.728778, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000548 DAOOQX010000000
VERSION     DAOOQX010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111215_length_1504_cov_2.728778"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLX53_08265"
     CDS             <1..184
                     /locus_tag="PLX53_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOY73112.1"
                     /translation="LQFLTADGTKVSVRPSGTEPKIKFYIAIRGDFSPNAGIDEQLAL
                     LDERISRIAAELKITR"
     gene            215..631
                     /locus_tag="PLX53_08270"
     CDS             215..631
                     /locus_tag="PLX53_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOY73113.1"
                     /translation="MKSYRKELWFETRNRRELINITGEVEKCLAESGISEGLALVNAM
                     HITSSVFINDDESGLHHDFEIWLEKLAPEKPYAQYRHNGFEDNADAHLKRTIMGREVV
                     VAITNGKFDFGPWEQIFYGEFDGKRRKRVLVKMIGE"
     gene            complement(674..1048)
                     /locus_tag="PLX53_08275"
     CDS             complement(674..1048)
                     /locus_tag="PLX53_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOY73114.1"
                     /translation="MKKKLMVILLLTTALAASAQDFVGSAPEWVNNPPKSVRKFYGVG
                     VGTSTTPEMAEEKAKMDAKTAIATQAGAVKVENIKSDKGVTQRKIVNATLSEVKMVKK
                     ACMKDGERYTVWVLMEMKGKRK"
     gene            1289..>1504
                     /locus_tag="PLX53_08280"
     CDS             1289..>1504
                     /locus_tag="PLX53_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOY73115.1"
                     /translation="MDSVRELSAKYSPAGIEDKWYRYWLEKGFFSSTPDEREPYCIVI
                     PPPNVTGVLHMGHMLNNTLQDVLIRRAR"
BASE COUNT          377 a          327 c          346 g          454 t
ORIGIN      
        1 gctgcaattc cttactgccg atggaacaaa ggtttcggtt cgtccttcgg gcaccgagcc
       61 caaaataaag ttctacattg cgattagggg cgattttagc cccaatgctg gcattgacga
      121 gcagcttgcg cttttggatg aaagaatttc acggattgct gccgagctaa agattacccg
      181 gtaattttgc ctatgtgcag gttaaacctt atttatgaag tcgtacagaa aggaattatg
      241 gtttgaaaca cgcaacaggc gtgagctgat taacattacc ggggaagtgg agaagtgcct
      301 ggctgagagc ggtatttccg aggggctggc attggtcaac gccatgcaca tcacttccag
      361 cgtattcata aatgatgatg agagcgggct tcatcacgat tttgagattt ggcttgagaa
      421 gctggctccg gaaaaaccat acgcccagta ccggcacaac ggttttgagg ataatgccga
      481 tgcacattta aagcgaacca tcatgggacg cgaggtggtg gtagccataa ccaatggtaa
      541 gttcgatttt ggaccctggg agcaaatttt ttacggcgag tttgacggta aacgacgaaa
      601 aagggtgctg gttaagatga ttggggagta atccagatta cagctctttg atttccgaag
      661 tgtcctttag ccgctatttt cgttttccct tcatttccat caaaacccaa acggtgtacc
      721 gctcaccatc cttcatgcaa gcctttttta ccattttaac ctccgaaagg gtggcgttaa
      781 caatttttct ttgggtaacc cccttatccg atttaatatt ttcaaccttt actgcaccag
      841 cttgtgtggc gatagcggtt ttggcatcca tttttgcctt ttcctcggcc atttccggcg
      901 tagtagatgt tccaacacca acaccataga attttcttac cgattttggt gggttattaa
      961 cccattcagg cgctgaacct acaaaatcct gtgctgaggc agctagggct gtcgttagta
     1021 ggagtataac cattagtttc tttttcattc cggttaatat taaaggttat aagccattta
     1081 ctagttgtac aaccactaat tttttttaaa atttactcaa acctatttcc gatttttata
     1141 catcacattt tgtgccattg cattgtgcca ggctctagtt tccccaatcc ttggaaaatt
     1201 ttaattgctc gggtaggtag gatgctccat gcgttgagct ttcaaaattt agctattttt
     1261 accgttcgat ttttttttga tctagcctat ggatagcgta agagaactgt cggccaagta
     1321 cagtccggcc ggaattgagg ataagtggta caggtactgg ttagaaaagg gtttctttag
     1381 ctcaacgccc gatgagcgtg aaccctactg cattgtaatt cctccaccaa atgttacggg
     1441 tgttttgcac atggggcaca tgctcaacaa cacccttcag gatgttctca tccggcgggc
     1501 gagg
//
LOCUS       DAOOQX010000549         1500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_111724_length_1500_cov_2.528720, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000549 DAOOQX010000000
VERSION     DAOOQX010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111724_length_1500_cov_2.528720"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="PLX53_08285"
     CDS             <1..910
                     /locus_tag="PLX53_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HOY73116.1"
                     /translation="LLTTILILIFFHFGVVHNLAHALIGLAIVLIISFLFTTVAANAI
                     AIVGTNPVSGMTLMTLIITSLVMASVGLSGTSGMVAALIIGGVVCTALSMAGGFITDL
                     KIGYWLGSSPFKQQTWKFLGTFVSAATVGGVIMLLNKTYGFTGKDALVAPQANAMAAV
                     IEPMMSGKPAPWLLYAAGAFFSLILTMIKVPALPFSLGMFIPLQLNTPLLVGGIISHI
                     VSTRSKDAAVNNARKDRGTLVASGFIAGGALMGVISAILRFFNVNYVNAQWFESHSAE
                     LLGLVMFLILCGYLIWESLRAKPEGE"
     gene            1108..>1500
                     /locus_tag="PLX53_08290"
     CDS             1108..>1500
                     /locus_tag="PLX53_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HOY73117.1"
                     /translation="MIEQQVVDRIIAAADILEVLQDFVTLRKRGVNYLGLCPFHNEKT
                     PSFTVSPSKGIYKCFGCGKGGNVVNFIMEHEHLGYVDALKFLAKRYNIEIVEKEATPE
                     ELEQRNERESLMVVSAYVQKYFSNILHKH"
BASE COUNT          374 a          367 c          348 g          411 t
ORIGIN      
        1 cctgctaacc accattctca ttcttatttt cttccacttt ggcgtggttc ataaccttgc
       61 tcacgccctg atcggtctgg ccatagttct tataatatcc ttccttttca ccaccgtagc
      121 ggccaacgca atagccattg ttggaaccaa ccctgtttcg gggatgaccc tcatgacgct
      181 catcatcacc tcgctggtaa tggcttcagt agggctttcg ggaacgtcgg gaatggtagc
      241 cgcactcatt ataggaggcg tggtttgcac cgccctgtca atggcaggag gtttcatcac
      301 cgatttgaaa atcggctact ggttgggttc atcacctttt aaacagcaaa catggaagtt
      361 tcttggtact ttcgtatcgg ccgccacggt tggcggagtt atcatgctgc tgaataaaac
      421 ttacggtttt acaggcaaag atgcgctggt ggccccacag gctaacgcca tggctgccgt
      481 tatcgaacct atgatgagcg gtaaacctgc tccctggctg ctctacgctg ccggagcttt
      541 cttctccctg atacttacca tgattaaggt tcccgccctc cctttctccc ttggtatgtt
      601 catcccgcta cagcttaaca ccccattact ggtaggtgga ataatttccc atattgtttc
      661 tacacgctca aaggatgctg ctgttaacaa cgctcgtaag gacaggggca cgctggttgc
      721 ctctggattc atcgccggag gcgcattaat gggtgttatt agcgccatcc tgagattttt
      781 taatgtgaac tatgtaaatg cccaatggtt cgaatcgcac tcggcggaac tgctcgggct
      841 ggtaatgttc ctgatactct gcggctacct gatttgggag agccttaggg ccaaacccga
      901 aggggagtag caactcattt ctatactctc gatagcggct accctgccat cttgctggct
      961 gtgtatccgc tatctttttg ttatccagca tgttatatgg ccaaagcaac caatatgcaa
     1021 agctgctttt ttaacaattc tatcggtcaa aaagaaatga aaaaaacatc aaagttccta
     1081 cctttaccat agaaataaag cagaaagatg attgagcagc aagtagttga caggataatt
     1141 gccgcagcag acatactgga ggtcctacag gatttcgtta cccttcgtaa acggggagtt
     1201 aactacctcg ggctatgccc atttcataac gaaaaaacac cttcgtttac cgtcagccca
     1261 tcaaagggaa tttacaagtg ctttggttgc ggaaagggcg gtaatgtggt taacttcatc
     1321 atggagcatg agcatttggg ctacgtggat gcgctaaagt tcctggccaa aaggtacaat
     1381 atcgagattg ttgaaaagga ggctactccc gaagagctgg aacagcgaaa cgagcgcgaa
     1441 agccttatgg tggttagcgc ttatgtacaa aagtacttca gcaacatttt acataagcat
//
LOCUS       DAOOQX010000550         1498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_111954_length_1498_cov_3.742204, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000550 DAOOQX010000000
VERSION     DAOOQX010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111954_length_1498_cov_3.742204"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..>1498
                     /locus_tag="PLX53_08295"
     CDS             132..>1498
                     /locus_tag="PLX53_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOY73118.1"
                     /translation="MKRRYLLPIWLILFIFPSLISAQNQKGAKYVFLFIGDGMGIQQV
                     NAAQVYADSVLHDTLGVTFMQFPVTTLATTYAANRYVTCSAAAGTALSTGYKTSIGTL
                     GLNANHTDTLYSIATRFRDNGRKVAILTSVSIDHATPAAFYAHAGSRSSYYQIALDLL
                     KSGYNFYGSGGFLEPNGSKHQGSTVASIYELGTRAGYGFTSSFTGVDSLKMLGVKSIV
                     YSTPNAAPEQTLQYEIDRKTSDISLADLTRKAIEVVDNPKGFFMMVEGGKIDWACHDN
                     DAATAIHEVLALSRAVNVALDFYSKHPSETLIIVTADHETGGMSLGNNANGYDLHLTA
                     LSLQKASADRLQAELYDLWSKNPKATFEDYLGLLEARIGLSENGAFALTADEMNTLEA
                     AYKRLVNLNAGESKNAQKNAGLLAGDAISILNRKAGIGWTSHSHTGSPVPVFIMGRGS
                     SQLPG"
BASE COUNT          411 a          353 c          335 g          399 t
ORIGIN      
        1 caaaccgatt aggctagcaa gcctcacaat agcaattctt gttaatcaac tttaacatca
       61 taatagctta tagctaatag ttttgttaat aattccgact aattttgttt aaaatactaa
      121 tctaaactat catgaaaaga cgatacttac tgcctatttg gttaattctg ttcatatttc
      181 cgtctcttat ttctgcccag aatcagaaag gggcgaaata cgtatttttg tttattggcg
      241 atggaatggg cattcagcaa gttaatgctg cccaggtata tgccgacagc gtgctacacg
      301 atacgctggg ggttaccttc atgcaattcc ctgtcacaac ccttgcaaca acctatgctg
      361 ccaaccgata cgtaacctgc tccgcagcag ccggaactgc cctatcaacg gggtataaaa
      421 catctatcgg gacgttaggg ctgaatgcaa accataccga caccctttat agcattgcaa
      481 ccaggttcag ggataacggt cgtaaggttg ctattcttac cagtgttagc atagatcacg
      541 ccacacctgc tgccttttat gcacatgcag gctcccgaag cagctactac caaattgcct
      601 tggatttgct caaaagcggg tataactttt atggctccgg tggtttcctt gaaccaaatg
      661 gctccaaaca ccaagggagc actgttgctt ctatttatga gttgggaact agggcgggct
      721 atggctttac ctcttctttt accggtgtag attcccttaa aatgctgggc gtaaaatcaa
      781 ttgtttactc aacgccaaat gccgcaccgg agcaaaccct tcagtacgag atagatcgta
      841 aaacctcgga catttcactg gctgacctta cccgcaaggc aattgaggtg gttgataacc
      901 ctaagggctt ttttatgatg gtagagggtg gtaaaattga ctgggcttgc cacgacaacg
      961 atgctgctac cgcaatccac gaggtgcttg ctctatccag ggcagtgaac gtggcgttgg
     1021 atttttacag caagcatcct tcggaaaccc tcattattgt tactgccgat cacgaaacag
     1081 gcggaatgtc gttaggaaat aatgccaatg gctacgattt gcacctgaca gcgctaagcc
     1141 ttcaaaaggc atcggccgac aggcttcaag ccgaacttta cgatttatgg agtaaaaatc
     1201 ctaaagccac cttcgaggat taccttggcc tgctggaggc caggatcggg ctttcagaaa
     1261 atggtgcatt tgcgttgact gctgatgaaa tgaacaccct tgaagctgcc tataagcgct
     1321 tagtcaacct aaatgcaggg gaatcaaaaa atgcgcaaaa aaacgctgga ttacttgcag
     1381 gtgatgccat ttctatactc aacagaaaag caggaattgg ttggacaagc cactcccaca
     1441 cgggctcgcc tgtgccggtt tttataatgg gtcggggatc atcgcaactt ccgggatt
//
LOCUS       DAOOQX010000551         1494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_112490_length_1494_cov_3.318277, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000551 DAOOQX010000000
VERSION     DAOOQX010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112490_length_1494_cov_3.318277"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="PLX53_08300"
     CDS             <1..154
                     /locus_tag="PLX53_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HOY73119.1"
                     /translation="EVKGYPILNLDVWEHAYYLKYQNRRPEYIQNWWNVVNWSAVEKR
                     FLEGLK"
     gene            302..>1494
                     /locus_tag="PLX53_08305"
     CDS             302..>1494
                     /locus_tag="PLX53_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY73120.1"
                     /translation="MNRLFKYFTFLILAIPTGLPRMAAAMVNKATPDTLQINGNLNTV
                     RSLITSREFSAAAMLTKEALSNSRKINYIKGVAWSEYYLGMICFENSTLDSAIYWLKR
                     SENDFAAVRDYSARVKACNLLGIYYRQSDSNDSSLDCHTRALKIAMENNDSAGIAESH
                     YGIGMTLNQLGYYPETMEHFHAAISIREKLNDSSGVAAILNSMGILFRNQGDYANALD
                     FYVRALNIHEKLGNIQGLAYQHNNMGLIYRDIGDYGKALDHFNKSWALKVKINDSRGI
                     SNSIMNIGSVYLLQNKLDSALIFFDQAASIKEQIGDKSGIANINLFRGECYQKLGLYK
                     KALDFLESSRSDYHALKEPRGETEAIIQLASTYFNSGMHDKAFQLIDEAKNMVVTLKM
                     LDLQQ"
BASE COUNT          457 a          311 c          318 g          408 t
ORIGIN      
        1 ggaggtaaaa ggctacccca ttctcaacct cgatgtatgg gaacatgcct actatcttaa
       61 ataccagaat cgtcgccccg aatacattca gaactggtgg aatgtggtga actggtcggc
      121 cgttgaaaaa agatttttag aaggcttaaa ataacctgtt agggtcattt tgttttgtta
      181 tttggttttg aggctgggac aatttccccg cctctttttt tgtactgctg tttttatttt
      241 ggttatactt ttgtaaattt aagcttataa aatatgctat tctgcctata ataagctgct
      301 gatgaataga cttttcaaat actttacctt tttaatttta gctatcccca cgggcttgcc
      361 aagaatggct gcagccatgg taaataaggc tacaccggac accctgcaaa ttaatggaaa
      421 tctgaatacg gttaggtcgc taataacctc ccgagagttt tcagcagctg caatgctcac
      481 caaagaagcc ctcagcaatt caagaaaaat caactacatt aaaggtgttg catggagtga
      541 gtactacctg ggaatgatat gttttgagaa ttcaacacta gattctgcaa tatactggct
      601 aaaacgatca gaaaatgatt ttgctgccgt tcgcgactat agtgcacggg taaaagcatg
      661 caacctgctg ggaatttact atcgccaatc cgatagcaac gacagctccc tggattgcca
      721 cacccgagct ttaaagattg ccatggaaaa taacgactcg gccggtattg ccgagtcgca
      781 ctatggaatt ggtatgaccc tcaaccagct cggctattac cccgaaacca tggagcattt
      841 tcacgcagcc atctcgataa gggaaaagct aaatgacagc tcaggagtag cggcaattct
      901 gaatagcatg ggcatcctct ttagaaatca gggcgattat gccaatgcgt tagatttcta
      961 tgtccgggca ttgaacattc atgagaagtt gggaaatatt cagggattag cctaccagca
     1021 caacaacatg ggtctaattt acagggacat tggcgattac ggtaaggcac tcgatcattt
     1081 caacaaatca tgggcattga aagttaaaat aaacgatagc cggggaattt ccaactcaat
     1141 tatgaatatt ggttcggttt accttttaca gaacaagctg gacagcgcac taatattttt
     1201 tgatcaggcc gcctccataa aggagcagat aggtgataag agtggaattg ccaatattaa
     1261 tttattcagg ggtgaatgct accaaaaact tggtctttac aaaaaagctt tagactttct
     1321 cgaatcatcc agaagtgact accatgctct aaaagaaccc cggggtgaaa ccgaagcaat
     1381 catccagctg gcttcaacat actttaactc gggtatgcac gacaaggcct ttcagctgat
     1441 cgatgaagct aaaaatatgg tagtaacatt gaaaatgctc gacctgcagc agaa
//
LOCUS       DAOOQX010000552         1494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_112493_length_1494_cov_3.250869, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000552 DAOOQX010000000
VERSION     DAOOQX010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112493_length_1494_cov_3.250869"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="PLX53_08310"
     CDS             <1..1301
                     /locus_tag="PLX53_08310"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOY73121.1"
                     /translation="QKRFPTSWVEGRGWNQNEWKVREFPTNELLNRAFPDKPVYIVRI
                     DGHAAIANNAALKIAGVTASTRVEGGEVVLQKGKPTGLLLDNAMELVRRIIPKPTIDE
                     KAAALQNAQKKCFAVGLTGVQDAGLDVDEVLLIDSLQQSGKMEIWVNAMLNPTPANYE
                     RFISKGVYRTNKLTVRSIKLYADGALGSRGALLLEPYSDDRGNRGLQVEPTEKLDSLC
                     GMAFKAGYQVCTHCIGDAAVKLMLDIYSKYLPAGNILRWRIEHAQVVDRNDLPRFGSL
                     AVIPSVQTTHATSDMDWADERLGSRIKNAYAYQKLLLQNGWIANGSDFPIESINPLYG
                     FYAGVARKNLEGKPDGGFQPENALTRKQALCAMTIWAAKAAFCEDARGSLEPGKAADF
                     VILNTDLMNDELPAIPRATILKTFVSGKEVFGSNAFSHGN"
     gene            1291..>1494
                     /locus_tag="PLX53_08315"
     CDS             1291..>1494
                     /locus_tag="PLX53_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73122.1"
                     /translation="MEIRQAQKQQLIEVLYIIRECSNQLVEKGVNFWSNSHADMEEIS
                     SDIEQGYVYVMVNNHVSIATVTLK"
BASE COUNT          408 a          358 c          386 g          342 t
ORIGIN      
        1 accaaaagag atttccaaca agctgggttg agggtagggg ttggaaccag aacgagtgga
       61 aggttaggga gttccccacc aacgagctgc ttaaccgggc attccccgat aaacctgttt
      121 acattgttcg gattgacggc catgcggcca ttgcaaataa tgctgctttg aaaattgccg
      181 gcgttactgc cagcacacga gttgaaggcg gtgaagtggt tctgcaaaaa ggaaaaccta
      241 cggggttgct gctcgacaat gctatggagc tggtgcgtag gataattccc aagccaacta
      301 tagatgaaaa agccgctgcg ttgcagaatg ctcagaaaaa atgctttgcc gttgggctga
      361 ccggagttca ggatgcaggg ctcgatgttg atgaggtgct gcttattgac tcgctgcagc
      421 aatccggcaa aatggaaatc tgggtgaatg ccatgctcaa tccaaccccg gcaaactacg
      481 aaaggtttat ttcaaaaggg gtgtatcgga ccaacaagct taccgtccgg tcaataaagc
      541 tatacgccga cggggcgttg ggctcaagag gcgccctgct tcttgaacca tactctgacg
      601 atcgtggtaa ccgtggcctt caggttgaac caacggaaaa actcgattcg ctctgcggca
      661 tggcatttaa agccggatac caggtgtgca cccactgcat tggcgatgct gctgttaagc
      721 tcatgcttga catctactca aaatatttgc ctgcaggaaa tatccttagg tggcggattg
      781 aacatgcaca ggttgttgac aggaacgatt tgccacgttt tggttcactt gccgtaatcc
      841 cctcggttca aaccacccat gccacctcgg atatggactg ggcagatgaa aggctgggca
      901 gcagaattaa gaatgcctat gcctaccaga agctactgct tcaaaacggc tggattgcca
      961 acggaagcga tttcccaatt gaaagtatca acccgcttta cggtttctac gctggcgttg
     1021 cccgtaaaaa cctggaaggc aaacccgacg gaggatttca acccgagaac gcccttaccc
     1081 gaaaacaggc cctctgcgcc atgactatat gggctgcaaa agccgctttc tgcgaagatg
     1141 ctaggggtag ccttgagcca ggaaaagctg ccgattttgt tattctcaac accgacctga
     1201 tgaacgatga gctgccggca attccaaggg caaccatcct gaaaaccttt gtctccggca
     1261 aggaggtttt tggttctaac gcttttagcc atggaaatta gacaagcaca aaaacagcag
     1321 ctgattgagg tgctctacat cattcgggaa tgctccaacc agctggttga aaaaggggtt
     1381 aacttctgga gcaacagcca tgccgacatg gaggaaatat caagtgatat tgaacagggc
     1441 tacgtgtatg tcatggtaaa caaccatgtt tccattgcaa ccgtaacctt aaaa
//
LOCUS       DAOOQX010000553         1494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_112536_length_1494_cov_2.396803, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000553 DAOOQX010000000
VERSION     DAOOQX010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112536_length_1494_cov_2.396803"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLX53_08320"
     CDS             complement(<1..295)
                     /locus_tag="PLX53_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOY73123.1"
                     /translation="MSYVTKEKLFSKAWFKAYTLITIGAFVLAAGFVLFIDPHRIAPG
                     GVYGIGIIVHYLTQGVFSWAPEGFPIGTVGLLLNIPLTIIGIKILGPRFGVKTV"
     gene            complement(897..>1494)
                     /locus_tag="PLX53_08325"
     CDS             complement(897..>1494)
                     /locus_tag="PLX53_08325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOY73124.1"
                     /translation="VVSSDFDYLFDEARLPDDISAYLGYKSGTWIGVAPFAKHKGKIY
                     PLNLMEAVVSRLAANKSYKIVLIGGRNFEKELLEEWAKKYTNTFSVAGKYSILNELKI
                     ISNLDVLISMDSANMHLASLVNTPVVSIWGATHRFAGFYGWKQNADNTVEVELSCRPC
                     SVYGNKPCFRKDYACLNRITPEMIISKVNSVVDRYGRI"
BASE COUNT          464 a          316 c          254 g          460 t
ORIGIN      
        1 ccaccgtttt aacaccaaaa cgtggcccaa gaattttaat tcctataatg gtaagcggaa
       61 tgttgagtag caggcctacc gtcccaatcg gaaatccttc gggcgcccag gaaaacacgc
      121 cctgggtaag gtagtgtaca attattccga ttccgtaaac acctccaggt gcaattctat
      181 ggggatcgat aaatagcacg aacccagcag ctaacacaaa tgcaccaatt gttattaggg
      241 tgtaggcttt aaaccaagcc ttactaaaca acttttcttt cgtaacgtac gacatacaat
      301 atttattttg ctgcaaaggt aagtaaaaaa caccatgcaa gtggctgtgg cattaaaact
      361 tgcccgctta agtaaaaatt aaaatatttt tattcgggtt attttctggt tgttgtgatt
      421 tatgattaaa tatttactac agtgtttgaa aagcttatct ccaatattta catttaaaga
      481 acgggtaata aaggtgttgg gttaatcgct ggttgagcca cttagtgcgc tatccatcca
      541 ttcagaaatt ttattttttt gtggtagccc tcgaaccgta atccctccgt tagttcgatt
      601 atatcgtctg aaattgtttt cacaaaatat tagcactttt aaacaatacc aaccatggtg
      661 gtgatgggta aactaatcag aaagacaacg gagtgctata aatcataatt acattataat
      721 atgctcataa cctgtaatat taacattttt atatgatgtt tgagttcaga taatcaatat
      781 ttttcttctc atttatatta attaaagtta tatatctctt tatcattcaa cagtataaaa
      841 ttaaatttaa cgataaaccc attcagctgt caactttagg ctaaatcatg gatttttcaa
      901 attcttccat atctatcaac cactgaattt accttactaa taatcatttc gggggtgatt
      961 cggtttaaac atgcgtagtc cttacggaaa caaggcttgt tcccatacac cgagcaaggg
     1021 cgacaggaca actcaacttc tacggtgtta tcggcgttct gcttccaccc gtaaaatccc
     1081 gcaaaacgat gggtggcgcc ccaaatgctc acaaccggag tgttaacaag cgaagcaagg
     1141 tgcatattgg cagagtccat ggatataagc acgtccaaat tagaaataat tttaagctcg
     1201 tttaaaatac tgtatttacc ggcaactgaa aatgtatttg tgtatttttt tgcccactcc
     1261 tccagcaact ccttttcaaa atttctacca ccaataagca ctatcttata actcttattt
     1321 gctgccaatc tcgacacaac ggcttccatc aggttgagcg gatatatctt ccctttatgc
     1381 tttgcaaaag gagctacacc tatccaggtg ccacttttat accccaggta tgcactaata
     1441 tcatcaggca accgggcctc gtcaaataag taatcgaaat cgctactaac gacc
//
LOCUS       DAOOQX010000554         1493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_112607_length_1493_cov_3.659944, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000554 DAOOQX010000000
VERSION     DAOOQX010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112607_length_1493_cov_3.659944"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PLX53_08330"
     CDS             <1..717
                     /locus_tag="PLX53_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOY73125.1"
                     /translation="KLKIVVRAGAGYDNLDLAACSAKGIVAMNTPGQNSNAVAELAIG
                     LMIYMARNLFQPGTGTEISGKKLGIHAYGNVGKLVAKYGKGFGMAVCAFDPFVSKDEM
                     EKDGVKAVDSIDTLYSKCDYVSLHIPANDKTKKSIGYELLSKMPKGATLINTARKEVI
                     DEEGLKKALAERPDLKYATDIMPDCHAELVEKFPGRVFATPKKMGAETAEANVNAGLA
                     AARQIVAFIKNGDKTFQVNK"
     gene            1155..>1493
                     /locus_tag="PLX53_08335"
     CDS             1155..>1493
                     /locus_tag="PLX53_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOY73126.1"
                     /translation="MVKIKSFKGIRPPKELVKEIAARPYDVLNSAEAQKEAGEKSLLH
                     ITKPEIDFNPIIDEHSKEAYDKAVENFRKWKEKGWLVQDKKENYYIYAQTMEGRTQYG
                     LVACCHYEDYM"
BASE COUNT          461 a          296 c          358 g          378 t
ORIGIN      
        1 aagctcaaga ttgtggtacg ggcaggtgcg ggctacgaca atctcgattt ggctgcttgc
       61 tcagccaagg gaattgtagc catgaataca cctgggcaaa actccaacgc agttgccgag
      121 cttgccattg gccttatgat atacatggct cgtaaccttt tccaaccagg aacaggcacc
      181 gagatttcag gcaaaaaact tggtattcat gcctacggca acgtgggtaa gctggtggct
      241 aagtacggca agggatttgg aatggctgtt tgcgccttcg acccgtttgt atccaaggat
      301 gaaatggaga aggatggggt aaaggctgtt gactcaattg atacacttta ctcgaagtgc
      361 gactacgttt cgctgcacat acctgcaaac gacaaaacaa agaagtctat cgggtacgag
      421 ttactctcca agatgcccaa gggcgctaca ctaatcaaca ctgcccgcaa ggaggttatt
      481 gatgaggagg ggttgaaaaa agccttggcc gaacgtcccg atttgaaata tgccaccgac
      541 attatgcccg attgccatgc cgagctggtg gaaaaattcc ccggaagggt gtttgcaaca
      601 cccaagaaaa tgggtgccga aactgctgaa gccaacgtta acgctggcct ggctgctgcc
      661 cgtcaaatag ttgcctttat caagaatggc gataaaactt ttcaggtgaa taagtaataa
      721 caggtgaaaa gttaaaagtg aaaagttaaa cgagaaggtg gagcatacta agctagaggc
      781 ttggaaagag gctgtagagt taacagttga aatttatgaa ctaactaaat cttttcctaa
      841 agaagagact taggggttaa cctctcagat tagtcgagca gtagtttcta ttccatcaaa
      901 tattgctgtg ggctgtgcaa gaaatagttc ggcagaaaca aattcatttt ctttattttg
      961 cagctgaatc tttagccgaa ttagaaacac agctaataac ttagaatagg ctagggtata
     1021 tatcggatat atcagaaatt atgaagcata ttcttttagt taagcaatta aattttggcg
     1081 tgattaaaag ccttaaaatc aaccgtttaa cattattgtc ttttaacttt tcaactctta
     1141 acaattaact tttcatggta aaaataaaat cattcaaggg cattcggccc cctaaggagc
     1201 tggttaagga aattgccgct cgcccgtatg atgtgcttaa ctcggctgaa gctcagaagg
     1261 aagccggtga gaaatcgctg cttcacatta ccaagcccga aatagacttc aacccaatta
     1321 ttgatgagca ttccaaggaa gcctacgaca aagcggttga gaatttcagg aagtggaagg
     1381 aaaagggctg gctggttcaa gataagaagg agaactacta catctatgct caaaccatgg
     1441 agggtcgtac gcagtacgga ttggttgcat gctgccatta tgaggactac atg
//
LOCUS       DAOOQX010000555         1490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_112988_length_1490_cov_3.206969, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000555 DAOOQX010000000
VERSION     DAOOQX010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112988_length_1490_cov_3.206969"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLX53_08340"
     CDS             <1..555
                     /locus_tag="PLX53_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOY73127.1"
                     /translation="ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKFE
                     QEYERTPSPEELAELLDISKDKINDTLRISGRHVSVDAPFVDGEDNSLLDVIVNNDSP
                     NADKGLINESLSKEIERALATLTERERDIVRYFFGISCQEMTLEEIGEKFNLTRERVR
                     QIKEKAIRRLRHTSRSKLLKSYLG"
     gene            769..>1490
                     /gene="dapF"
                     /locus_tag="PLX53_08345"
     CDS             769..>1490
                     /gene="dapF"
                     /locus_tag="PLX53_08345"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOY73128.1"
                     /translation="MLITFSKYHGTGNDFVMVDNRSGGLNPTVDLVEKLCNRHFGIGA
                     DGLILLENAKESALMMRYFNSDGRESTMCGNGGRCFAHFAHQLGLAGQHVLFNGIDGF
                     HEAYLLDNGNIRLRMIDVPVVESAEEGFFLNTGSPHFVVFVRSVENVNVDLEGRLASK
                     SVNVKNGGTNVNFIQPVGEKVLKIRTYERGVEAETLSCGTGSVASAIAYADFFAISEG
                     PVEQKNQHMQLLKPLSRFRNCI"
BASE COUNT          390 a          300 c          381 g          419 t
ORIGIN      
        1 atatcctatg cagtttggtg gattcgccag tcaattcttc aggctttggc agaacagtcc
       61 agaattgttc gcctgcccct aaaccaggtg ggttcgttaa ataagattaa caaggctttt
      121 tctaagtttg aacaggagta tgaaagaact ccctcacctg aggagctggc cgagctactt
      181 gatatctcaa aggataaaat taacgatacc cttcgtatca gtggtcgcca tgtttccgta
      241 gatgcacctt ttgtagatgg cgaggataac agcctgcttg atgttattgt taataacgat
      301 tctccaaatg ccgataaggg gctaattaac gagtcgttga gtaaggaaat tgaaagggca
      361 ctagccaccc ttaccgagcg cgaacgtgat attgtcaggt atttttttgg catcagctgc
      421 caggagatga ccctggagga aattggcgag aagtttaacc ttacccgtga acgggtacgt
      481 cagattaagg aaaaagccat acggagactt cgtcatacct cacggagtaa gctgttgaag
      541 tcttatctag gttagcctat tttgctggat gatttgtttg ataactaaaa gccccctgtg
      601 cggggctttt tttgttacct gttggtgggg tgaagttgat gaatgttctc ttactcctgc
      661 attctaatcg ggatatgacc gattggggaa gaggtcttaa cccgatgtca acattattgg
      721 aaaaaatcac ctaactttca ccaaaaattt taaaaattca cacgtgctat gctgataacc
      781 ttctcgaaat atcacggaac gggcaacgat ttcgtaatgg ttgacaaccg ctcaggaggg
      841 ttaaacccta cagttgactt ggttgaaaag ctctgtaaca ggcattttgg aattggagct
      901 gatgggctaa ttcttctgga gaacgccaag gagagtgctc tcatgatgcg ctacttcaac
      961 tccgatgggc gtgagtcaac catgtgcggg aatggtggcc ggtgttttgc ccatttcgcc
     1021 caccagcttg ggttagccgg gcagcatgtg ctcttcaacg gaattgatgg ttttcacgaa
     1081 gcctacctgc tcgataatgg aaacattcgg ttacgtatga tagatgtacc ggttgttgag
     1141 tctgctgagg aaggcttttt cctcaatacc ggttcgcccc actttgtcgt ttttgtacgt
     1201 agtgttgaga acgttaacgt tgaccttgag ggtaggcttg ccagcaagtc ggtaaatgtg
     1261 aagaatggtg gaaccaacgt taattttatt cagccggtag gtgagaaggt attgaagata
     1321 agaacgtatg agcggggagt ggaggcagaa accctttcat gcggaaccgg ctcagtggct
     1381 tcagcaattg catatgctga tttttttgcg atttccgaag ggccagttga gcaaaaaaat
     1441 cagcatatgc aattgctgaa gccactgagc cggttccgca attgcatatg
//
LOCUS       DAOOQX010000556         1489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_113092_length_1489_cov_3.728731, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000556 DAOOQX010000000
VERSION     DAOOQX010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113092_length_1489_cov_3.728731"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PLX53_08350"
     CDS             complement(<1..462)
                     /locus_tag="PLX53_08350"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY73129.1"
                     /translation="MTATAIPFRKKLALNLFKTYRRNAAKLHELNYVLWEATLRCNLS
                     CLHCGSDCKMDASVGDMPADDFLRVMDQVAEVANPNSTMVVFTGGEPLLRNDLEQVGL
                     SLYRKGFPWGIVTNGMLLSKQRIDSLVDSGLRSVTVSLDGLEASHNWLRGSG"
     gene            complement(459..899)
                     /locus_tag="PLX53_08355"
     CDS             complement(459..899)
                     /locus_tag="PLX53_08355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM-associated putative lipoprotein"
                     /protein_id="HOY73130.1"
                     /translation="MKKAKRIILVSYNVLITCLMAILGFLSSCRDTPADEYGSPHALF
                     KAKGNVSSAAAQQPIEGIKVTSQIDTVFTGIEGQFEINAGVDLPTGQSFNLKFQDSDG
                     AANGEFQELDTIVEFNNPVFTGGDGHWYQGETEKEFNVQLKGKE"
BASE COUNT          402 a          395 c          315 g          377 t
ORIGIN      
        1 ccccgagccc ctcagccaat tgtgcgaggc ctccaacccg tcaaggctaa ccgtcacaga
       61 cctaagccct gaatcaacta aggagtcgat ccgctgcttg ctcagcaaca tcccgttggt
      121 tacaattccc cagggaaaac ctttccggta aaggcttaac cccacctgtt ccaaatcatt
      181 tcgtaaaaga ggctcaccac cggtaaacac aaccatggtg ctgttgggat tggcaacttc
      241 ggcaacctgg tccattacac gcaagaaatc atcagcaggc atatcgccta cagaggcatc
      301 cattttacaa tcgcttccgc agtgaaggca actcaggttg cagcgtaggg ttgcctccca
      361 aagaacgtag tttaactcgt gcagctttgc cgcattacgc cggtaggtct tgaaaaggtt
      421 aagggctagc tttttcctaa aagggatagc ggtagctgtc attctttccc cttcagctga
      481 acgttaaact ccttttcagt ctcaccctgg taccaatggc catcgccacc agtaaaaacc
      541 ggattattga actcaacaat ggtatccagc tcctgaaact ctccgttcgc cgctccgtcg
      601 ctatcctgaa acttaaggtt aaacgattgc ccggtaggta aatccactcc ggcgttaatt
      661 tcgaactgcc cttcgattcc ggtgaacacc gtatctatct gcgacgtcac cttaataccc
      721 tcaatgggct gttgggctgc agcagacgaa acatttccct ttgccttaaa cagggcatgt
      781 ggagaaccgt actcgtcggc cggggtatcc ctgcatgagg ataaaaatcc caaaattgcc
      841 atgaggcagg taatcagcac gttgtaggaa acaagaatta tacgcttagc tttcttcata
      901 gctgacatat taaactacaa tttaagcaag ttaaaaaaaa acaggacaaa ccagtaagtg
      961 ccgggtaaaa tattgaaagg aaataaaaaa attgtcacca aaagtgctgt ggagaagcaa
     1021 gataccacac gaaataattt atacctgagt gaataaaaat cccttagcta ccttacggtt
     1081 gtcctcggcg ttttccgccg gctggtggat cggttcgtga cgtgtggcaa tcccatccct
     1141 actctcaaca acatactaag cccatcctta cttttgtagc tgataatccc gctgaattat
     1201 cttgttacta cccattactt tctccctact accacttact ttttttcttc ttcgacacta
     1261 ccacgcgaga ggactaactt cgttgagctc cctacggtcg gttcgtgagg ttgcatgggt
     1321 ccaacggtcg acggtatgaa acgtgccgga ttgctgggct gcggctctgt cccccgtgaa
     1381 aaacttacga ggcgggacgg aacgctcgaa tacccactaa aaccggcatg ttttatgacc
     1441 gcgtgttgtg gccagtaatt ttttcatctt aatataaaac taatcggaa
//
LOCUS       DAOOQX010000557         1486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_113437_length_1486_cov_3.231307, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000557 DAOOQX010000000
VERSION     DAOOQX010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113437_length_1486_cov_3.231307"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..555)
                     /locus_tag="PLX53_08360"
     CDS             complement(22..555)
                     /locus_tag="PLX53_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73131.1"
                     /translation="MKKKISTVLMLCALAFGFASCESDDSDSYDNYITISGSVTIDGK
                     TYSNPTFNLGTSSEIQGFLYQMEVGKNQTSYIDISPKAGTISSVDGLNIHYYLVLEGD
                     KPGNAGLTAMILFNKNDENIFTLESVQVQATITKIDEVGGFIEATYSGSFTDHNTSSQ
                     TYSAQGKIKAKRIQPPI"
     gene            complement(827..>1486)
                     /locus_tag="PLX53_08365"
     CDS             complement(827..>1486)
                     /locus_tag="PLX53_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOY73132.1"
                     /translation="HLGDMDFKVAGTRDGITATQMDIKVEGLSYEVLSKALKQAHEGR
                     MHIMNIMLNTIPAPRADFKPHAPRIEQFEIPRDLIGAVIGPGGKVIQEIQRETSTTIN
                     IEEANNHGIVSVFAENAENRDAAVKWIKSIVAVPEVGEVYRGKVKSIMPFGAFVEILP
                     GKDGLLHISEIEWKRLEKVEDVLKEGDEIDVKLIDVDKKTGKLKLSRKVLLEKPATPK
                     K"
BASE COUNT          372 a          315 c          328 g          471 t
ORIGIN      
        1 caggatgccc tggctttctt gctatatagg tggttgaatt cgttttgcct taatctttcc
       61 ttgggcgctg taggtttggg acgatgtgtt gtgatctgta aacgatccgg agtacgtagc
      121 ctcaatgaat cctcccactt catcaatttt ggtgattgtg gcttgcactt gaaccgactc
      181 aagagtaaaa atattttcgt cattcttgtt gaacaaaatc atagcagtta gtccggcatt
      241 tcctggttta tccccctcta aaacaaggta gtaatgaatg ttaaggccat caacagagct
      301 aattgtgcca gccttgggtg aaatgtcaat gtagctggtt tggtttttcc caacctccat
      361 ttgataaagg aatccctgaa tttcactaga agtgccaagg ttaaaggttg gattgctgta
      421 ggtttttccg tcaattgtta cactcccgga aatagtgatg tagttgtcgt agctatcgct
      481 gtcatcgctt tcgcacgaag cgaatccgaa tgctaatgcg caaagcatta aaactgttga
      541 gatttttttc ttcatagagc attatgtatt ggttttagct aaaatagcaa gttttagctt
      601 aacagttttt caattttgac gagtggacat tttagccata catgtagcgc aattttaagg
      661 atgtgtggcg atgggtatag gttaatttca tgatgttgag gttaatccaa aaaaacctga
      721 ctttaagagg gaaaatggga ttgcaggagg taaaattcgt aaaaaaaacc tgccagaagt
      781 ttgctggcag gctttttaat actatgagtc atttactttg aatcacttac tttttaggtg
      841 tggcgggttt ttcgagcagc accttgcgtg agagtttaag cttgccggtt tttttatcaa
      901 cgtcaatcag cttaacatca atctcatcgc cttccttcag aacatcctcc accttttcca
      961 ggcgcttcca ttcaatttcc gagatgtgta gaagtccgtc ttttccgggt aatatttcca
     1021 caaacgcccc aaaaggcata atggatttaa ccttccccct gtaaacctcg cctacttcag
     1081 gaaccgcaac tatgctctta atccacttaa ctgcagcatc gcgattttcg gcattttcgg
     1141 caaatacgct tacaattccg tggttgttag cctcctcaat gttgatggtg gtggaggttt
     1201 cacgctgtat ttcctgaatc actttacctc cgggaccaat aacggcacca atcaggtccc
     1261 tgggaatttc aaattgctct attcggggcg catggggttt gaagtcggca cggggagccg
     1321 gaatggtgtt tagcatgata ttcataatgt gcatgcggcc ttcatgagcc tgcttaagag
     1381 cttttgaaag cacttcgtag gaaagtcctt ccaccttaat atccatttgg gtggcggtta
     1441 tcccatcacg cgtgccggca accttaaagt ccatatcgcc caggtg
//
LOCUS       DAOOQX010000558         1485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_113586_length_1485_cov_2.990210, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000558 DAOOQX010000000
VERSION     DAOOQX010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113586_length_1485_cov_2.990210"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PLX53_08370"
     CDS             <1..457
                     /locus_tag="PLX53_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73133.1"
                     /translation="SVKLLPASSKTLRDLPIRLLLLLITMPVALYGAVNNAIPIAVPY
                     LLSKKFKDVQFHSSIKHAGALLLTPLAYLVQTLVVGFVLKNIWLALAYLATLPLGAAA
                     FYLWRREFFRFTSQLSLRRFRKAKAEEFDLVNHLNNQIYRTVTGLVDKE"
     gene            complement(630..>1485)
                     /locus_tag="PLX53_08375"
     CDS             complement(630..>1485)
                     /locus_tag="PLX53_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73134.1"
                     /translation="LVAQKVQRFATVEDLKKHKCTVEEMEEYEPHVVRGNVIYAGVDY
                     EAILREAEKDPSGCDVILWDGGNNDFSFYKPDLTITVADPHRPGNEVSYYPGEVNLRM
                     ADVVVINKMDSAAPEGIQMVRDNIRRVNPKAIVVDGASPIKVDNPELITGKRCLIVED
                     GPTLTHGEMKIGAGTIAARKFGASEEVDARPFLVGKLKETYQIYPNIGRILPAMGYGE
                     QQLKDLEGTINATDCDSVIIGTPIDLNRIIKIKKPSTRVYYDLVEIGLPDLKGVLEKF
                     VKQHKLDK"
BASE COUNT          380 a          370 c          321 g          414 t
ORIGIN      
        1 ttccgtaaaa ctcctgcctg cttcctcaaa aacccttcgg gatttaccaa tcagattgct
       61 cttgctgcta attaccatgc cagttgccct gtatggggcg gtaaacaacg ctattcccat
      121 agctgtcccc tacctgctca gcaaaaagtt caaggatgtt cagtttcaca gctctataaa
      181 acatgccggg gcgttgcttt taacccccct ggcatatcta gtacaaaccc tggttgttgg
      241 atttgtgctg aaaaacattt ggctagcttt ggcctacctg gccacgctac cccttggtgc
      301 tgctgccttt tacctatggc gaagggagtt cttccgattt acctcccagt tatcgctacg
      361 aaggttccga aaagctaaag ctgaagagtt cgacttggtt aaccatctta acaaccagat
      421 ttacagaact gtaacaggtt tggttgacaa agaataggct acaaaacttc ccattctcca
      481 agatcaagat ttagaaaagg ctttagtctc taaatggcca atatgggcca catttaggta
      541 tgattttttt caccgcaagg catcactaaa aagaattaag gcagccttgt tgagccgcct
      601 taaccattat acctcaaaaa tactgcttac tacttatcaa gcttgtgctg tttcacaaat
      661 ttctcgagta ctcctttgag gtcgggaaga ccaatttcaa ccaaatcgta gtaaactcgg
      721 gtgcttggtt tctttatttt gataatgcga ttaaggtcta ttggtgtgcc aataatcacg
      781 ctatcgcaat cggtagcgtt gatggttcct tccaaatctt tgagctgctg ctcaccgtag
      841 cccattgcag gaagaatccg gccaatgttt gggtaaatct ggtaggtttc cttaagcttt
      901 ccaacgagga acggacgggc atccacctct tcggaagccc caaacttacg agctgcaatg
      961 gtgccggcac ctatcttcat ctcgccatgg gtgagggttg ggccatcctc tacaatgagg
     1021 cagcgtttgc ctgtaatcag ctcggggtta tcaactttaa tgggtgaagc gccatcaaca
     1081 acaatggcct tggggttaac cctacggatg ttatcacgaa ccatctggat accctcagga
     1141 gcagcggaat ccattttatt aattacaact acatcagcca tacggaggtt aacttcgccg
     1201 ggatagtagc ttacctcgtt gcctggacgg tgagggtcgg caacggttat ggtcaaatcg
     1261 ggtttgtaga acgaaaagtc gttgttgcca ccatcccaaa ggattacatc acaaccgctt
     1321 ggatcctttt cggcttcacg aagaatggcc tcgtagtcaa cgccagcata gataacattc
     1381 ccgcgaacaa cgtggggttc gtactcctcc atttcctcaa cggtgcactt atgcttttta
     1441 aggtcttcaa ccgtagcaaa ccgttgtacc ttttgggcaa ccaaa
//
LOCUS       DAOOQX010000559         1483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_113813_length_1483_cov_3.738095, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000559 DAOOQX010000000
VERSION     DAOOQX010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113813_length_1483_cov_3.738095"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLX53_08380"
     CDS             <1..647
                     /locus_tag="PLX53_08380"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOY73135.1"
                     /translation="YNSHLRFGIQTSGIVRTRNYSGLVFPDMIDINGDVTGTYNTSGI
                     TTWNYDFSLGVAGDFENFYGGIAVHHLLEPVEATYQDTKAYIPRKYTVHIGADFNLYK
                     WYKFKEVLLLSPNIIYIQQAKFNQLNIGFYLSRHWLVGGFWLRENLSLNSHSFIFTIG
                     YTDDLFRMGYSYDFSIMKYGLRGLPTSSHEVTLGWNFEYKKGKSKYKYIKCPKF"
     gene            727..>1483
                     /locus_tag="PLX53_08385"
     CDS             727..>1483
                     /locus_tag="PLX53_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOY73136.1"
                     /translation="MNLKNKPLSLILVAMLGLTSCGISGKGGSNVSSKTGWAYNSPDE
                     GGFEVVTDWKPEAGPGLVFIEGGTYIMGRVEQDLMYDWNAKPRRVTVTSFYMDETEVS
                     NVDWREYIYWLKRVYSDYPQVIKQALPDTLVWRSPLGYNEPFVTDYFRHPAYNDYPVV
                     GVNWLQANDYCQWRTDRVNEMLLVQKGILEIELGQKGDDVFNTEAYLAGQYVGKVKKN
                     LPSLNPEQPEGRPVRFEDGILLPKYRLPTEAEWE"
BASE COUNT          437 a          297 c          344 g          405 t
ORIGIN      
        1 cctataactc acatttgcgt tttggtattc aaacaagtgg cattgtgcgt acccgtaact
       61 actcggggct tgtatttccg gatatgatcg atataaatgg agatgtaacc ggtacttata
      121 atacttcggg aataacaaca tggaactatg atttcagcct tggagtagcg ggggattttg
      181 aaaattttta tggaggaata gctgttcacc accttctgga gccagtggag gcaacctacc
      241 aggacacaaa agcttacata ccacggaagt acaccgttca tattggcgct gacttcaacc
      301 tgtataaatg gtacaaattt aaagaggttc tcctgctgtc accgaacata atatacattc
      361 aacaagccaa gtttaaccag ctaaacattg ggttctatct ttcccggcac tggttggtgg
      421 gaggattttg gctaagggaa aacctaagcc tgaacagcca ttcatttatt ttcacgatcg
      481 ggtacaccga cgatttgttt agaatgggct atagctacga cttcagcata atgaagtatg
      541 gccttagagg attaccaacc tcatcgcatg aggtaaccct gggatggaat tttgagtata
      601 aaaagggaaa aagtaaatat aagtacatta aatgtccgaa attttaattt tttacgttac
      661 tacattgtgg taaacaattt tttatcacta tatttgaact tattttaagg gtaaaaataa
      721 atagttatga acttaaagaa taaaccatta agccttattt tggtagccat gttggggctt
      781 acttcatgtg gaatttctgg taaggggggg tcaaacgtat catcaaaaac cggatgggca
      841 tacaattcgc ccgacgaagg tggttttgaa gttgttaccg actggaaacc ggaagccggc
      901 ccaggattgg tgttcattga gggtggaacc tacattatgg gtcgggttga acaggactta
      961 atgtacgatt ggaatgccaa gcccagaagg gtaaccgtaa cctctttcta catggatgaa
     1021 acagaggtta gtaacgttga ctggcgggaa tacatatact ggcttaaacg cgtttattcg
     1081 gactacccgc aggtaataaa gcaggctctt ccagatacgc tggtttggcg atccccattg
     1141 ggctataacg aaccattcgt taccgattac ttccgtcacc ctgcatataa cgactatccc
     1201 gttgttggcg taaattggct tcaggcaaat gactactgcc agtggcgtac cgatcgggta
     1261 aatgaaatgc tgcttgttca aaagggaatt cttgaaatag aactcgggca aaagggtgat
     1321 gatgttttca acaccgaagc ctaccttgcc ggtcaatatg tgggaaaagt taagaaaaat
     1381 ttgccaagcc ttaaccccga gcagcccgaa ggccgtccgg ttcgctttga agatggcatt
     1441 ctgctgccaa aataccgcct cccaaccgaa gccgagtggg aat
//
LOCUS       DAOOQX010000560         1481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_114104_length_1481_cov_3.141655, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000560 DAOOQX010000000
VERSION     DAOOQX010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114104_length_1481_cov_3.141655"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /gene="rmuC"
                     /locus_tag="PLX53_08390"
     CDS             complement(<1..830)
                     /gene="rmuC"
                     /locus_tag="PLX53_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOY73137.1"
                     /translation="MTGIVLVGILVVLAVVGISILLIRKPTVDIKPQLREVENLMVGF
                     GATLDRVEKSIKDEFQRNRAETNEISKTNREELTKSLDTFSSTFEKSVKELNDVLKAK
                     YDELRNQQADFNRQISDANKANREELANSLKSFETKFTENIRELNELLRQKFSDFSQQ
                     QTEVNRQLTENIKDVKQSVDNQLKSIREDNTHQLDEMRKTVDEKLQKTLNDRLSQSFE
                     TVGKQLQAVQVGLGEMKNLAADVGGLKRVLSNVKMRGGIGEIQLSMLLEQILAPDQYE
                     A"
BASE COUNT          360 a          349 c          337 g          435 t
ORIGIN      
        1 gcttcgtact ggtcgggtgc aagaatctgc tccagcagca tggagagctg gatttcacct
       61 attcccccac gcattttcac gttgcttagc acccgtttta gtccacctac atcggcagca
      121 aggtttttca tttcgcccag ccccacctgt acggcttgaa gctgcttgcc aaccgtttcg
      181 aatgactggc taagcctgtc gttaagtgtt ttctgcagct tctcatcaac tgttttccgc
      241 atctcgtcga gctggtgggt gttgtcctca cgaatggatt tcagctggtt atcaaccgat
      301 tgcttcacgt cctttatgtt ttccgtcagc tggcggttca cctcggtctg ctgctgcgaa
      361 aagtcgctga atttctgacg aagcagctcg ttcagctccc ggatgttctc ggtaaatttt
      421 gtctcgaacg atttaaggct gtttgccagc tcctcgcggt tggccttgtt ggcatcggaa
      481 atctgcctgt tgaaatcggc ctgctggttc cgtagctcat catacttggc tttaagcacg
      541 tcgttaagct ccttaacgct tttttcaaaa gtgctgctga aggtatcgag cgatttggta
      601 agctcctccc ggttggtttt tgaaatttcg ttggtttccg cgcggttccg ctgaaattcg
      661 tcctttatgg atttctctac tcggtcgagg gtggcgccaa aacccaccat caaattttca
      721 acctcacgca gctggggctt aatatccact gtgggcttcc taatcagcag tatggatatg
      781 cccaccacag ccaacaccac cagaattcca accagaacaa ttcctgtcat agcttaattt
      841 tttaaagtca acatgtatgc ctgaataccc gtagcaggtt gttaaggtaa cctccacaat
      901 tatcgccggc ttaaaggtat aaaaataagc ccttgggcgt attctaaata tcgaattact
      961 ttttaggtgg cagctaattt tgcagccggg catgtgaaag aggtgggtgc ccatcgttcg
     1021 gcttatgccc ttgctaggct taggttttaa cttaagccga aaattaacaa acaagcttca
     1081 gcctgctagc agggtgaatc agacgataga aacaataatg aatagacctg ccgtgtaata
     1141 gtaacgaccc atgcagctat ttggaaagtt aagtagcatg gacactcctt tccatgtggc
     1201 aatcctgttg actgctggaa aaaccggtca ctttagggcg gattttagca tttcattatc
     1261 ggccataggt agcagctgca ccaccaggat ggtttaagcg gtagccgatt tgcggtgcat
     1321 tcaaaaatca gtcattgcca atgatttaaa agaccacaaa aagttgaaac ccacgtaagc
     1381 aaaagtttaa ctaaagccag cggcacacct ggagagttgt gaaaaagtaa atgtgtttgg
     1441 tttattgaat gagattttat attacttttg aatttaaggg t
//
LOCUS       DAOOQX010000561         1476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_114741_length_1476_cov_2.822660, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000561 DAOOQX010000000
VERSION     DAOOQX010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114741_length_1476_cov_2.822660"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PLX53_08395"
     CDS             <1..418
                     /locus_tag="PLX53_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOY73138.1"
                     /translation="TGAIAHGMLVADRLNLPFVYVRSSPKSHGLANRIEGEYRREQKV
                     VVIEDLISTGQSSIAAVEALKEAGCNVLGLLAIFSYQLEQADENFTSAGVEFTTLTNY
                     SELIGVAVSTGVVNASQLQLLMSWHENPESWGNITL"
     gene            502..1026
                     /locus_tag="PLX53_08400"
     CDS             502..1026
                     /locus_tag="PLX53_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOY73139.1"
                     /translation="MPIIGTKYRLFIFVVESNPDCVARLMQGLEVPEQSLIKAFSSGE
                     SFIDFFPKSKIPKRKLILIFLGYTFSDEANQNMMNGIEILETVKMLNPAVHVVMLTHN
                     GEQEYGGHAKSMGANSCIMKDESMVIRANSLIRLFLSEKKLGNRRRQLKVAAVSWLVA
                     LVCFLLSVGVSCFS"
     gene            1220..>1476
                     /locus_tag="PLX53_08405"
     CDS             1220..>1476
                     /locus_tag="PLX53_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY73140.1"
                     /translation="MQTGKIGVTTENIFPIIKKFLYSEHEIFLRELISNAVDATQKLK
                     TLASVGDYKGEVGDTTIRVKLDKKAGTLTISDSGVGMTADE"
BASE COUNT          415 a          310 c          358 g          393 t
ORIGIN      
        1 taccggtgca atagcccacg gaatgcttgt tgccgacagg cttaacctgc cgttcgttta
       61 tgttagatcg tcacccaaat cgcatgggct agccaacagg attgaagggg aataccgtcg
      121 tgagcaaaaa gtggtggtaa ttgaagacct catttcaacc ggtcagagca gcattgcggc
      181 ggttgaagcc ctaaaggaag ctggctgtaa tgtgctgggg cttttggcta tattcagcta
      241 ccagcttgaa caagctgatg aaaattttac cagcgccggt gttgagttta ccactcttac
      301 gaactatagc gaacttatag gggtggctgt cagtacaggc gttgtgaatg ccagccagct
      361 gcagctcctg atgtcgtggc acgaaaaccc tgaatcatgg ggaaatataa ccttgtaggc
      421 tgaatttctc tttggcgggc ataaatatat ttcgttcatt ttattacatt tatcctaatt
      481 ttcaactaaa cgggtgctag catgcccatc attggcacga aatacagact cttcatattt
      541 gttgttgaga gcaaccccga ttgtgttgcc aggttgatgc aaggcctgga agttcctgaa
      601 caatccctaa taaaggcatt ctcgagcgga gagagtttta ttgatttttt ccccaagtcc
      661 aaaatcccca aaagaaagct aattctgatt tttttgggct ataccttttc cgatgaggcc
      721 aaccaaaaca tgatgaacgg tatcgaaatt ctagaaaccg ttaaaatgct taaccctgct
      781 gttcatgttg taatgctaac ccacaacggc gagcaggagt acggtggtca tgcaaaaagc
      841 atgggggcca acagctgcat tatgaaggac gaaagcatgg taatcagagc caacagcctt
      901 atcaggttat ttctaagcga aaagaaattg ggcaataggc gacgtcagct taaagttgca
      961 gcggttagct ggttagttgc gctggtttgc tttttactct ccgttggtgt tagctgcttt
     1021 tcctagccaa cctgccaaaa ttgcgcctac cgtgtccgct tggcagttgt attaagaatc
     1081 acccgacaaa gtgtaattat caataaatca acatgatgat aatcattttc tatgccctac
     1141 ccggtggtaa attttgtatg gcattcaact tgcaggtaga ggctggaaaa aatttgttaa
     1201 acctttaaat gtggatgcaa tgcaaacggg taaaattggg gtaacaacgg agaatatttt
     1261 cccgatcatc aaaaagtttc tctattccga gcacgagata tttctgaggg agctcatatc
     1321 gaatgccgtt gatgctacac agaagttgaa aacgctggca tcggtgggcg attacaaggg
     1381 cgaggtgggc gatacaacca tcagggttaa gctcgataaa aaagctggaa ccctcaccat
     1441 cagcgatagc ggggtgggca tgacagccga cgagat
//
LOCUS       DAOOQX010000562         1473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_115134_length_1473_cov_2.658674, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000562 DAOOQX010000000
VERSION     DAOOQX010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115134_length_1473_cov_2.658674"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1473)
                     /locus_tag="PLX53_08410"
     CDS             complement(<1..>1473)
                     /locus_tag="PLX53_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73141.1"
                     /translation="SYTLLLVLIFQVNAIWAQTTGRFPVMNFHHREYNGHAQSWAITQ
                     DNRGLIYIANNVGVIEYDGSEWRHISINGALARCLDTDKNGRIWVGGQDEFGYLAADS
                     TNTLSFHSLSQHIPETAKPLGMIRQLYASDSSIYFSSFTTLFRLNKDLTVSEWSPKTQ
                     FHRSYLTDGVLFIHQRDYGLCYLSNDSLRLVPGGDYFAPQLIYAILPFNQKYFLICTQ
                     NDGFYLLSRQALDPNFTGYTKPAIVKFSTSDDNFFASSKVYAGLKLDDNTFAISTYSG
                     GVVFIDTTGNITRRIDTSVGLQDNTAWYLYMDNQKNLWIALNNGISYTPIFSQLTSWT
                     ENDGIKGVPQSVVRFKNHIYFTTNVGIFWFSNGRIEQVTGLSDLSWKLRTIRTMNGDE
                     ALLATTSSGIFQVEGGKATLIENGKYHSYCLLQSKLYPKITYVGLREGVGVIAENNGR
                     FKFLGELTGTKGEVYSLAEDLNGDLWYSERYKGINYVDMV"
BASE COUNT          394 a          326 c          339 g          414 t
ORIGIN      
        1 taaccatatc aacgtagttg atacccttgt accgttcaga gtaccatagg tctccgttta
       61 aatcttctgc cagggagtaa acctccccct tagttccggt cagctccccc aaaaatttga
      121 accgtccgtt gttttctgct attacaccga caccttcgcg taacccaacg taggttattt
      181 tcgggtacag cttcgattgg agaaggcagt aggaatggta cttcccattt tcaattagtg
      241 tcgcctttcc tccttctacc tgaaaaattc cacttgaggt ggtggcaagc aaggcctcat
      301 cgccattcat tgtgcgtatg gttcgcagct tccatgataa atcggataaa cccgtaacct
      361 gctctatcct gccgttgcta aaccagaaaa taccaacgtt ggtggtgaag tagatgtggt
      421 ttttaaaacg gacaaccgat tgtggaacgc ccttaattcc atcgttttca gtccagctgg
      481 taagctggga gaaaatgggt gtgtaggata tcccgttgtt tagtgcaatc caaagatttt
      541 tttggttatc catgtataga taccatgcag tattatcctg taggccaacc gaagtgtcaa
      601 tgcgtctggt tatgttgcct gtagtatcaa taaataccac tccaccgctg taggttgaaa
      661 tggcaaaggt attatcatct agtttaagcc ctgcatacac cttggaggat gcgaaaaaat
      721 tatcgtcaga agtggaaaat ttaacaatag caggctttgt atatccggtg aaatttgggt
      781 ctagagcttg cctgctgagc aggtaaaagc catcgttttg agtgcaaatc agaaagtatt
      841 tttggttgaa tgggagtatt gcgtatataa gctgcggggc aaagtaatct cctcccggaa
      901 ctaggcgcag ggagtcattg ctcaggtagc aaagcccata gtcgcgctgg tggataaaca
      961 acacaccatc ggtaaggtag gagcggtgaa actgtgtttt tggcgaccat tccgaaacgg
     1021 ttaaatcctt gtttaacctg aatagagtag taaaagatga aaagtaaatt gatgaatcgc
     1081 tggcgtagag ctgacgaatc attccgagcg gcttggccgt ttcgggtata tgctgggaaa
     1141 gggaatggaa gcttagggta ttggtcgaat cggctgcaag gtatccaaat tcatcctgac
     1201 caccaaccca aattcttccg tttttgtcag tatcaaggca gcgtgctagt gctccattaa
     1261 ttgaaatatg tcgccactcg ctcccatcat actctataac cccaacgttg ttggcaatgt
     1321 atattagccc ccggttatcc tgcgttattg cccagctttg agcatgccca ttatactccc
     1381 tatggtgaaa gttcatcacc ggaaaccttc cggtggtctg agcccaaatg gcattaacct
     1441 gaaatatcag aaccagaagc agtgtataac ttg
//
LOCUS       DAOOQX010000563         1469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_115611_length_1469_cov_3.004243, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000563 DAOOQX010000000
VERSION     DAOOQX010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115611_length_1469_cov_3.004243"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /gene="gldL"
                     /locus_tag="PLX53_08415"
     CDS             <1..867
                     /gene="gldL"
                     /locus_tag="PLX53_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOY73142.1"
                     /translation="ATAAPQISVQHAGAGAGGALIFTEKFNQMLENAEISPALFDKVS
                     QGLNKLSQASSSIADITTTAVHVKEFTDNMAKASESVKTFHENYSQNSQVLNESMNHL
                     SDSIQKTAGEMSESGKNFMDGVAGSVQNLENELTAAGQKVGGRIVESCETVAGKLNMA
                     AEGLANAYQQTAEGVAITYQQLAEAMKNNGSTITKGSTTYNQQLEQLNKNMAALNTVH
                     EMHLQETTQRLKEAEKLYQGVDGMIKKLNVTVDESEKFKSALETLNSNITSLNNVYGN
                     MLAAINSISTSK"
     gene            901..>1469
                     /locus_tag="PLX53_08420"
     CDS             901..>1469
                     /locus_tag="PLX53_08420"
                     /inference="COORDINATES: protein motif:HMM:NF023504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73143.1"
                     /translation="MAHGKETPRQKMIGMMYLVLTAMLALNVSTDVLEAFVLVDNGLS
                     RTTENYRLKNDKLYSEFDKAMLVNPVKVGPWKSKADEIKEMAREIYDYVQDLKIEVVK
                     TSEGDDTPAINGREVISELIEDKANLDIGGQILIGMEGNGKAFELKRKIIKFREKAIS
                     LLDPVRGEEMIKSINSILNTDDPPVREDGT"
BASE COUNT          469 a          330 c          360 g          310 t
ORIGIN      
        1 gccaccgcag ccccgcagat ctcggtgcaa catgctggag caggagctgg cggtgccctc
       61 atctttaccg agaagtttaa ccaaatgctc gagaatgccg agattagccc agccctattc
      121 gataaggtaa gccaaggact taataagcta agccaggcat caagtagcat tgcagatatt
      181 accacaactg ccgttcatgt taaggagttt accgataaca tggccaaagc ttctgaatca
      241 gttaaaacat tccacgagaa ctactcacag aatagccagg ttctcaacga atccatgaat
      301 cacctttcgg acagcattca gaaaaccgcc ggcgaaatgt ccgaatcggg caagaacttt
      361 atggatggag ttgccggttc ggttcagaat ttggagaatg agcttactgc tgcaggtcaa
      421 aaagtagggg gaaggattgt ggagtcatgc gagaccgttg ccggcaaact caacatggct
      481 gcagaggggc tggccaacgc ctaccagcaa acagccgaag gggttgcgat aacttaccag
      541 cagctggccg aggcaatgaa gaataacggt tcaaccataa ccaagggtag cactacctac
      601 aaccagcagc tggagcagct aaataagaat atggcggctc ttaacacggt tcatgagatg
      661 caccttcagg aaacaactca acggcttaag gaagccgaaa agctgtacca gggggtggat
      721 ggaatgataa agaagttgaa tgttaccgtt gatgaatccg aaaagttcaa atcagcactg
      781 gaaacactta acagcaatat cacgtctcta aataatgttt atggcaacat gctggcggct
      841 atcaactcga tttccaccag taagtaaatc aactgcatct taagactcac ctaacggtct
      901 atggcacacg gaaaagaaac tcccaggcaa aaaatgatcg gcatgatgta ccttgtgctg
      961 accgctatgc ttgcgctgaa tgtctcaact gatgttctgg aggcttttgt actggttgac
     1021 aacgggttat cgcgcaccac cgaaaactac aggctcaaga acgataaact ctactccgag
     1081 tttgataagg ccatgctggt taaccccgta aaagtcggcc cttggaaaag caaagctgat
     1141 gagataaaag aaatggccag ggagatatac gactacgttc aggatctcaa aattgaggtg
     1201 gttaaaacat ccgaaggaga tgatacacct gcaattaacg gtagagaggt cataagcgag
     1261 ttgatagagg ataaagccaa cctggatata ggagggcaga tcctaatcgg catggagggc
     1321 aacggcaagg cttttgagct gaagcgtaaa ataattaagt ttcgtgaaaa agccatttcc
     1381 ctactcgacc ccgttcgtgg tgaagaaatg ataaaatcca tcaatagcat tctaaacact
     1441 gacgatcccc ccgtaaggga agatggaac
//
LOCUS       DAOOQX010000564         1468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_115733_length_1468_cov_3.027601, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000564 DAOOQX010000000
VERSION     DAOOQX010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115733_length_1468_cov_3.027601"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1468)
                     /locus_tag="PLX53_08425"
     CDS             complement(<1..>1468)
                     /locus_tag="PLX53_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY73144.1"
                     /translation="ENEDIPGVTWKPSTDGVANPDQMISRTTEADMIRVRDHLINTYN
                     YDPGKYKNFDPFQNKNTKILARVDWNISKNHKFTIRYNDVVGTSDVETNFNSGPPNNA
                     RNSGRISSQSLAFSNSFYGFENTVRSITGELNSTFLTNISNKFLGSYTHIQDSRTSPS
                     KLFPFVDIWEDGDQYMSFGYELFSYNNDVINNTLSLLNNTTINLKKHTLTLGVSFDRL
                     YFKNSYIREGTTYYRYASVDDFINGADPIGFGVTYGYNGEDAPGAELTFGMGAFYAQD
                     EWKVNTNLKVTYGIRLELPFYFDDMKDNPAISALTFANSQKIDVGSWPDPQLLFSPRL
                     GFNWDVKGDRSLQVRGGTGIFTGLLPFVWFTNQPTNSGMIQSPEIGWGPGNANLVDLQ
                     FNPNFKEFIASNPPLFPQAPGTLPNNAGLAEVSKDFKMPQVWRSNLAVDVELPYNFVF
                     TGEALFGKDINAVQQININEANPTGNMVGPDNRPFWATA"
BASE COUNT          403 a          321 c          329 g          415 t
ORIGIN      
        1 ttgctgttgc ccaaaacggc ctattatctg gaccaaccat attgccagtt ggattggctt
       61 cgttgatgtt aatctgctgc acggcattaa tatctttccc aaataatgcc tcaccggtaa
      121 acacaaagtt atagggtagc tcaacatcaa ccgccaggtt ggaacgccaa acctggggca
      181 tcttaaagtc ttttgaaact tcagccaatc cggcattatt aggcaatgtt ccgggtgctt
      241 gagggaaaag cggaggattg cttgctatga attccttgaa gttgggatta aactgcaagt
      301 ccactaaatt cgcattgcca gggccccatc caatctcggg tgattgaatc atacccgagt
      361 ttgttggctg gttggtgaac caaacgaagg gtaaaagccc tgtaaaaata ccggtacctc
      421 ctcgaacctg caacgagcga tcgcctttaa catcccagtt aaatcctaaa cgaggggaga
      481 aaagcagctg cggatcgggc caactaccca catcaatctt ttgactattt gcgaaggtaa
      541 gtgcagaaat tgcagggtta tccttcatgt cgtcgaagta gaagggaagc tccaaacgaa
      601 tgccataggt aactttcagg ttggtattca ccttccactc atcctgagca taaaaagctc
      661 ccattccaaa agtaagctca gctccgggag cgtcttcacc attataccca taggttactc
      721 caaaccctat gggatcagca ccatttatga aatcatcaac cgatgcatac cggtagtagg
      781 ttgtcccctc ccggatgtat gaattcttaa agtaaagccg gtcaaaggaa accccaaggg
      841 tgagagtgtg tttttttagg ttaattgtgg tgttgttgag caaacttaac gtattgttga
      901 tcacgtcgtt gttatatgaa aacaactcgt atccaaagga catatactga tcgccgtcct
      961 cccatatatc tacaaagggg aacagctttg atggagatgt tctggaatcc tgaatgtggg
     1021 tgtagctacc taaaaacttg tttgaaatat tcgttaaaaa ggtgctgttt agctcaccgg
     1081 taatcgaacg aacagtgttc tcaaaaccgt agaacgagtt agagaaagcc agggattggc
     1141 tactaattct tcctgaattt ctagcgttgt tgggaggtcc actgttaaag ttggtttcaa
     1201 cgtccgaagt acccactacg tcgttatagc gaatggtaaa cttatgattt ttgctgatgt
     1261 tccagtcaac acgcgctaaa attttggtgt ttttgttctg gaagggatca aagtttttat
     1321 acttacccgg gtcatagtta tatgtgttga ttagatgatc ccttacccta atcatatcgg
     1381 cttcggttgt acgcgaaatc atctgatcgg ggttggcaac cccatcggtg ctgggtttcc
     1441 atgtaacccc aggaatatct tcattttc
//
LOCUS       DAOOQX010000565         1468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_115759_length_1468_cov_2.709130, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000565 DAOOQX010000000
VERSION     DAOOQX010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115759_length_1468_cov_2.709130"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="PLX53_08430"
     CDS             <1..891
                     /locus_tag="PLX53_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73145.1"
                     /translation="TPKDLSEQKIIAREIESIAYARKQLANPASHTIKPVNQNVNTRF
                     PEVNPLSDTTGNLLIYTSLQQFYNAILVSQRKEDLWTNPINLNAQLFADGAIKTVGIS
                     PNGNMLILARNDNDTYNLYFSRYDRTKNTWSPIEKFPKEINSSGWEISGSLSSRGDTL
                     YFSSNRAGGFGGFDIYMSVKTITGWSNPVNLGNKVNTPFDEISPFVTENGKQLFFSSN
                     GHRSMGGFDLYLSGYENKTWGLPHNLGYPINTLDDDTFLFPLNNGKRGFISRASEESS
                     GEDDIYEVTLDLSTLLNGDK"
     gene            1026..>1468
                     /locus_tag="PLX53_08435"
     CDS             1026..>1468
                     /locus_tag="PLX53_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73146.1"
                     /translation="MTILKKLLPVGICLCLTIAGKPQSKLEIEEIYRDANSYFYFEDY
                     EEALTLYLQVYSHQPENYNLCYRIGFCYLNIPGSKKNAIPYLEKATGNITRFYREESI
                     LETKAPPDAIFYLGNAYLVNNQVENAIKEYEKFWTDTRKQLAAAIP"
BASE COUNT          448 a          382 c          299 g          339 t
ORIGIN      
        1 actccaaagg atctctcgga gcaaaaaata attgccaggg aaattgagtc catcgcctac
       61 gccaggaagc agctcgccaa ccccgctagc cataccatta aacccgttaa ccagaatgtg
      121 aatacccgtt tccccgaggt taaccccctt tccgatacaa cgggcaacct gcttatctac
      181 acctctcttc agcagttcta caacgccatt ctggttagcc agcgcaagga agacctctgg
      241 accaacccca taaatcttaa cgcccagcta tttgccgatg gggctattaa aactgtgggc
      301 atttcaccca acggaaacat gcttattctg gcgcgtaacg ataacgacac ctataacctc
      361 tacttcagcc ggtacgacag aacaaaaaac acatggtcgc ccattgagaa gttcccaaag
      421 gagattaact ccagcggatg ggaaatttcg ggctcgctct ccagtcgtgg cgatacccta
      481 tacttcagca gtaacagggc aggtggtttt ggcggattcg acatctacat gtcggtgaaa
      541 accataacag gttggtctaa cccggttaac ctgggaaata aagtcaatac tcctttcgat
      601 gaaatatccc catttgttac cgaaaatggc aagcagctct tcttcagctc caacggacac
      661 cgctccatgg gaggattcga cctgtacctc tccggttatg agaataaaac atgggggtta
      721 ccccataacc tgggctaccc tatcaacacc cttgacgacg atacatttct attcccactc
      781 aacaacggta aaagaggatt tatttctcga gcctcggagg agtcaagcgg cgaggacgac
      841 atctacgagg taacccttga cctcagcacc ttacttaacg gggataaatg acggattgtt
      901 acaatagcgg cataaagcaa ctataacgtg tccaactcat atgaaaaatg ttttgagctt
      961 agagcctcat tccgcaggga caaacccgta aagaaatgaa cctttgaagg aaaacaagtg
     1021 catcaatgac aatcttaaag aaactcctac ctgttggaat ttgtctatgc ctcaccatag
     1081 ccggaaaacc acaatccaaa ttagaaattg aggaaatata tcgtgacgcc aactcctact
     1141 tttacttcga ggactacgaa gaggccctga cattatacct tcaggtatat agccaccaac
     1201 ccgaaaacta caacctttgc taccggattg gcttctgcta cctgaacatt ccggggagta
     1261 aaaagaatgc cattccctat ctggaaaagg caactgggaa tattacccgt ttttacaggg
     1321 aggagtctat tcttgagaca aaggccccac ccgatgcaat tttttacctt ggaaatgcct
     1381 acctggtaaa taaccaggtg gaaaatgcta ttaaggagta tgaaaaattc tggacagata
     1441 cccgcaaaca actggcagca gctattcc
//
LOCUS       DAOOQX010000566         1465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_116191_length_1465_cov_1.995035, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000566 DAOOQX010000000
VERSION     DAOOQX010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116191_length_1465_cov_1.995035"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..>1465)
                     /locus_tag="PLX53_08440"
     CDS             complement(99..>1465)
                     /locus_tag="PLX53_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOY73147.1"
                     /translation="YKKDFSPSIIRDVDKCIMCRRCETMCNQVQTVGALSAVNRGFMA
                     VVAPAFEQDLVKSPCTYCGQCVAVCPTGALTEVDHTAKVIRALADPTKTVMVQTAPAV
                     RAALGEEFGLEPGTLVTGKMVSALRKIGFNYIFDTDFAADLTIMEEGTELLDRLTRHL
                     NGDKSVKLPILTSCCPAWVNFFEHNYTDMLDVPSSAKSPQQMFGAIAKSYFAEKLGKK
                     REDLVVVSVMPCLAKKYECQRDEFKHEGNPDVDLSLSTRELANLIKQFNINFMELPDE
                     DFDRPLGESTGAAVIFGATGGVIEAAVRTAYELHTKKALPRVDFTELRGMDGIRTATI
                     DFDGLPIKIGIAHGLGNARKLLDQVRSGQADFHAIEIMACPGGCIGGGGQPLHHGDSS
                     IIKARMAAIYREDAGKSIRKSHENPYIIQLYEEFLGKPMSEKSHHLLHTQYFDKGKKD
                     IYIK"
BASE COUNT          367 a          371 c          335 g          392 t
ORIGIN      
        1 accgggatta ttcccaaagg atttgcagat ttcctttatt ttagccacct gatcgggctt
       61 aagttctact ttaatgtgcg acatgggttt cttcctccct atttaatata aatatccttt
      121 ttacctttat caaagtattg ggtatgaagt agatggtgtg atttctcact cataggttta
      181 cccaaaaact cctcgtaaag ctgtatgatg tagggatttt catgcgattt tcggattgat
      241 ttaccggcat cctccctgta aattgcggcc atacgagcct tgataattga tgagtcgcca
      301 tggtgtagag gttgtccacc tccacctatg cagcctccag ggcatgccat aatttcaatg
      361 gcatggaaat cagcctgtcc tgaacgaact tggtcaagca gcttgcgggc gttacccagc
      421 ccatgcgcaa ttccaatctt tatcggaagc ccatcaaaat caattgttgc agtccttatg
      481 ccatccatac cgcgaagctc ggtaaagtca accctgggaa gtgctttctt ggtatgcagc
      541 tcataagcgg tacgaacagc agcctcaatt acgccaccgg ttgcgccaaa tatcactgcg
      601 gctcccgttg attcgcccag tggtcgatcg aaatcctcat cgggcagctc cataaagttg
      661 atgttgaact gcttgataag gttggccagc tcgcgggttg acagcgatag gtcaacgtca
      721 gggttgccct catgcttaaa ctcatcacgc tggcattcat acttcttagc caggcagggc
      781 atcaccgata caaccaccaa atcctcacgt ttcttgccaa gtttttcagc aaagtacgat
      841 ttggctatcg ctccaaacat ttgctggggc gatttagcgc ttgagggaac atcgagcata
      901 tcggtgtagt tatgctcaaa gaagtttacc catgcggggc agcagctggt aagaatagga
      961 agcttaacgc tcttatcgcc attgaggtgg cgggttaacc tgtcgagaag ctcggttccc
     1021 tcctccatta tggtcaaatc ggccgcaaaa tcagtatcaa aaatatagtt gaacccaatt
     1081 tttcgaagtg ccgacaccat ttttcccgta acaagagttc ctggctccag gccaaactcc
     1141 tcgccaagag cagctctaac tgcaggggct gtttgaacca taacggtttt ggtgggatcg
     1201 gcaagcgccc ttattacctt ggcagtgtgg tcaacctcgg ttaaagcgcc agtagggcac
     1261 acagcaacgc attggccgca atatgtacag ggcgatttta ccagatcctg ctcaaaagca
     1321 ggggccacaa cagccataaa acccctattc acggcgctta gtgcaccaac tgtttgcacc
     1381 tggttgcaca tggtctcgca cctacggcac atgatgcact tatcaacatc gcggataatt
     1441 gagggcgaaa aatctttttt gtagg
//
LOCUS       DAOOQX010000567         1464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_116241_length_1464_cov_3.415188, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000567 DAOOQX010000000
VERSION     DAOOQX010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116241_length_1464_cov_3.415188"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="PLX53_08445"
     CDS             complement(<1..1086)
                     /locus_tag="PLX53_08445"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOY73148.1"
                     /translation="MSIATSQNRLKLSTFKLNALLGMAQAISAELKTEELVDRFRSIL
                     NDDLGIDRILLYKKEEDRWELLLNSNCPQKVVEGISVERDLMAFNEITFVGVAENPLL
                     LELDVIIPIVQNNAPVGYVLIGDTSEYEKGVSATIRHLNFVQTLSIIIFVAIENLRLF
                     HKSLEQEAMRKELELASKMQSMLIPTPESMPHYPHISIASFYNPHFEVGGDYYDVIPL
                     NDNEFGFCIADVSGKGISAALLMSNFQANLRALFSLDIPLTRLVEKLNERVMATANGE
                     KFITLFVGRYNVEKHLLEYVNAGHNPPFMIDKQSKIISTLSNGCVGLGMLDCIPVINQ
                     GAVTIDQPSLLVCYTDGLVEIMRNNKVE"
     gene            complement(1194..>1464)
                     /locus_tag="PLX53_08450"
     CDS             complement(1194..>1464)
                     /locus_tag="PLX53_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOY73149.1"
                     /translation="NADLLKEGQYVEMMVEADKENILTCELPPFVEMEVTYTEPGLKG
                     DTASSTALKPATVETGSVINVPLFVNTGDKIRIDTRTREYYERVK"
BASE COUNT          373 a          324 c          353 g          414 t
ORIGIN      
        1 ctccactttg ttgttgcgca taatttccac caggccatcg gtgtagcaaa ccagcaagga
       61 cggctggtca atggtaacgg ctccctgatt aataaccgga atgcagtcaa gcattcccaa
      121 gcccacacag ccgttggaaa gggttgaaat tattttgctt tgcttgtcaa tcatgaaggg
      181 tggattgtgc ccggcgttga cgtactccag gaggtgcttc tcaacgttgt atcgcccaac
      241 aaatagggta atgaattttt cgccattggc agtagccata accctttcgt ttagcttttc
      301 aactaatctt gtaaggggga tatccagtga aaaaagtgcc cgcaggttgg cctgaaagtt
      361 cgacatgagc agggctgcgg aaattccctt gcctgaaaca tctgcaatgc aaaagccgaa
      421 ttcattatcg tttaggggga taacatcgta gtagtcgccg cctacctcaa aatgtgggtt
      481 gtagaatgag gcaatggaaa tgtgggggta gtgtggcatg gactccgggg taggaattag
      541 catggactgc atcttggagg caagctcaag ttccttccgc atggcctctt gctccaaact
      601 tttatggaaa agcctaaggt tctctattgc cacgaaaata atgatggaaa gggtttgcac
      661 aaagttgagg tgcctgatgg tggcgcttac ccctttttcg tactcgctgg tatcgccaat
      721 caggacgtaa cccaccgggg cattgttttg aacgatggga atgattacat cgagttccaa
      781 tagcaggggg ttttcggcta ctccaacaaa ggttatttcg ttaaaggcca tcaagtcgcg
      841 ctcaaccgat atcccctcaa ccactttttg cgggcagttg ctgtttagaa gcaactccca
      901 gcgatcctcc tcttttttgt aaagtagtat cctgtctatt ccaagatcgt cgttaaggat
      961 tgatctaaac ctgtcaacca gctcctcggt tttcaactcg gcgctaattg cctgggccat
     1021 accaagtagg gcatttagct tgaatgtgct taactttaac ctattttgcg aggttgctat
     1081 agacatggtt taaaaaagta gcccaatcca acttggaaaa gtggaataag ttaacaataa
     1141 aaaaagggga agtcaaaatt ttatgaataa tttagcttgg atagcgatga aaactacttc
     1201 accctttcgt agtactccct tgttcttgtg tctatacgga ttttatctcc tgtattgaca
     1261 aataagggaa cgttgatcac cgaaccggtt tcaacggttg cgggtttcag ggcagttgaa
     1321 gaagcggtgt cgcccttgag gcctggttcg gtgtaggtaa cctccatttc cacaaacggg
     1381 ggaagctcgc aggttagtat gttctcctta tcggcttcta ccatcatttc aacgtattgt
     1441 ccttccttaa gcaggtcggc gttt
//
LOCUS       DAOOQX010000568         1459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_116935_length_1459_cov_2.319801, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000568 DAOOQX010000000
VERSION     DAOOQX010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116935_length_1459_cov_2.319801"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLX53_08455"
     CDS             complement(<1..485)
                     /locus_tag="PLX53_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOY73150.1"
                     /translation="MLEELYHHYLNHGSVATDSRSIPKGAIFFGLKGDKFDGNKFAEQ
                     ALNNGASLAVIDNPTFMTHDCILVENTLKTLQELANHHRKQLKAKIIGITGTNGKTTT
                     KELIQCVLSKKYHAVATKGNLNNQIGVPLTLLSLTADVEVAIVEMGASHPGDIAELAP
                     IA"
     gene            complement(731..>1459)
                     /locus_tag="PLX53_08460"
     CDS             complement(731..>1459)
                     /locus_tag="PLX53_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOY73151.1"
                     /translation="TYEERMVERRIYPWNGHNVRNSESKNRGQMMANFVRGRGDYMGL
                     AGNLNDQNTFPGPVKSYWPNDYGLYNMAGNVNEWVLDVYRPLSYEDFDEIRPFRGNVF
                     KTPYDKEGNFAKKDSLGRIPYREVTEEEAANRRNYNKAYYINYKDGDVASTLNYVEGE
                     TLKTKDSNAMYDQGKPDAKGAGMTSLINDHVRVYKGGSWKDRAYWLSPATRRYLDEAS
                     STNDIGFRCAMEAVGPQSARQRGE"
BASE COUNT          341 a          306 c          336 g          476 t
ORIGIN      
        1 gctattgggg ctaactctgc aatatccccg ggatggctag cacccatttc cacaatggca
       61 acttctacat cagccgtaag ggaaagaagc gttagcggaa ccccaatttg gttattcaaa
      121 tttccttttg tagcaaccgc atggtatttt tttgagagaa cgcattgaat cagctccttg
      181 gtggtggttt taccgttggt cccggttata ccaattattt tggcttttag ttgtttccgg
      241 tgatggttag cgagctcctg tagggttttt agggtgtttt caaccaaaat gcaatcatgc
      301 gtcataaagg ttggattgtc gattacagcc aaggatgctc cattatttaa tgcttgttct
      361 gcaaacttat tcccatcaaa tttgtctcct ttcaagccaa aaaatattgc acctttagga
      421 atgcttcggc tgtcggtggc aactgagcca tgatttaggt agtggtggta gagttcttca
      481 agcatgggtt gggtttttat atcctgtttt ttattattaa gttgattaac ttaggttctt
      541 tactgggtgt gtatgtggtt gatggtgatg gtaaactttt atagcttggt tgtagggtag
      601 gggtaatata caaatgtaag gcttgtcaat ggcatcggaa attcttttaa aggttatacc
      661 ttcaaaaagc agaaaacctc atgtttatga ggttttctcg atattaattg aagtgtttcc
      721 aaaaaaaaca ctactcgcct ctttgacggg ccgactgggg gccaacagcc tccattgcgc
      781 aacgaaatcc gatatcgttg gttgaagagg cttcgtccag ataccttctg gtggcgggac
      841 tcaaccagta ggctctatcc ttccaggaac cacccttgta aactctaaca tggtcgttaa
      901 tgaggctggt catgccggca ccttttgcat caggtttacc ctgatcgtac atggcgttag
      961 aatctttggt tttaagggtt tccccttcga cgtagtttag agtagaagca acgtcgccat
     1021 ctttatagtt aatatagtag gccttgttat agtttcgcct gttggcagct tcttcctcgg
     1081 taacctccct gtatggaatt ctacccaggc tatccttttt tgcaaagttg ccttctttat
     1141 catatggggt tttgaataca tttccacgaa atggtctaat ctcgtcaaag tcttcgtagc
     1201 ttaaaggacg gtaaacgtcg agcacccact cgttgacatt accggccatg ttgtaaagcc
     1261 cgtaatcgtt aggccagtag gacttaacag gacctgggaa ggtattctgg tcgtttaggt
     1321 ttccagcaag tcccatgtag tcgccgcgtc ccctgacaaa gtttgccatc atttgtcccc
     1381 tgttcttaga ttcagagttt ctaacgttgt ggccattcca tgggtagatg cggcgctcaa
     1441 ccattcgttc ttcgtaggt
//
LOCUS       DAOOQX010000569         1456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_117323_length_1456_cov_2.413990, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000569 DAOOQX010000000
VERSION     DAOOQX010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117323_length_1456_cov_2.413990"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="PLX53_08465"
     CDS             <1..1135
                     /locus_tag="PLX53_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73152.1"
                     /translation="TMFVERLSKSNKTIVALERVKLKESLGGIRMLSISNDSLLFIND
                     RLFNTISLFSAGSGKLVGNFTLDSAWYAAPYSKDTFKLRQIWMNQHVLERASYPQYYF
                     KGVFNHRGRTIISAICYYPFTTGKDFFILPKNLFFMLDTLLMPISYRWLYIDKETPIV
                     NFYNAVFFQDDNLITTTFDVVNNRLNFNPDFLYYFKPEGDSLIFDKSLHVEPPSFIKN
                     GYSMNGFSLNYISEYMGNITVWFSYYPEFFDLRLEKWVSIDTGYVDSKISTNTFEQGQ
                     PVNFQVLGVWAEANQYSMILRFGLQVYYMVQADKNIIQSALIYTLSEGNQLQMVRKSK
                     NEFVGTDYNSKTGDTEIIKFTINGVQTFISPSLNEACAFVVPM"
BASE COUNT          436 a          302 c          286 g          432 t
ORIGIN      
        1 aaccatgttc gttgagcgat tatccaaaag taacaaaacg attgtagcac tggaaagagt
       61 aaaacttaaa gagtcgctgg gtggaatcag aatgttgtcc atatcaaacg attcgttgct
      121 ttttataaat gacaggctgt ttaacaccat ttcgctgttt tcagctggca gcggaaaact
      181 agtcggaaat tttacgctag acagcgcttg gtacgcggca ccctatagta aggatacctt
      241 taagctaaga cagatatgga tgaatcagca tgtacttgaa agggcatctt acccccagta
      301 ctattttaaa ggagttttca accatagagg tagaactata atttctgcaa tatgctacta
      361 cccgttcaca actggtaaag acttttttat actacccaaa aacctttttt ttatgctgga
      421 cacactattg atgcccataa gctacaggtg gctttacatt gataaggaaa ccccgatagt
      481 taatttttat aatgctgtat tttttcaaga tgataacctc atcaccacca cctttgatgt
      541 tgtcaacaac cggctaaact ttaaccccga ttttttatac tattttaaac ccgaaggcga
      601 ctcgttgatt tttgataaat ccctacatgt agagccacca agttttatca agaatggata
      661 ttctatgaat ggtttttcat tgaactacat atctgagtat atgggcaata ttaccgtttg
      721 gttctcctac taccctgagt tcttcgacct aaggttggag aagtgggtat caatagatac
      781 aggctacgtt gattctaaaa tatcaactaa cacctttgaa cagggtcagc ctgttaattt
      841 tcaagtgctg ggcgtatggg ctgaagcaaa ccagtacagc atgattctaa ggtttggcct
      901 gcaagtttac tacatggttc aagctgataa gaacataatt caaagcgctc taatctatac
      961 ccttagcgaa gggaatcaac tccaaatggt tcgaaagagt aagaatgagt tcgttggaac
     1021 tgattacaac tcaaaaacgg gagatactga gattatcaaa ttcacaatta atggggtgca
     1081 aacatttatt tcaccctcgc tcaacgaagc ctgtgccttc gtcgtaccta tgtagcgttc
     1141 tctgacctca taagatacta tcagtaagct ttccattggg gtaaatataa acaatttcaa
     1201 gcggtttaac tttcgttcgg ttaaatttgt ggacatcagc ccactctgtt aattcgggct
     1261 ttgccaggta aatatagcta attgtaagtt ttccagcgta tgtcatttca aaaatagcag
     1321 gcgcagccag ctactgatag ccctgcggcc gcagaaccac cgccaaaagc gtttagatta
     1381 tttcaacctg taacatcagg cgggctgagg ctaggttagt gtttcccttc actaatccag
     1441 cccaaccttc aggggg
//
LOCUS       DAOOQX010000570         1450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_118101_length_1450_cov_2.471685, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000570 DAOOQX010000000
VERSION     DAOOQX010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118101_length_1450_cov_2.471685"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLX53_08470"
     CDS             <1..423
                     /locus_tag="PLX53_08470"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY73153.1"
                     /translation="IRAFGKGYEEFFQQSKGMGVNFIKGKVAKINQKDDNSGDLILRY
                     EDVTTGALKEANHDLVVLSVGVLPNNGITKVFTDDKLELDNQGFIKSIDELVSPSLTS
                     IDGVFVAGTAAGPKDIPDSILSAGSAASEAASYINSTL"
     gene            501..>1450
                     /locus_tag="PLX53_08475"
     CDS             501..>1450
                     /locus_tag="PLX53_08475"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY73154.1"
                     /translation="MMKQRIGVYICHCGGNISDYVDVEQLGKLMAKEDGVIISKDVVF
                     ACSDSTQKDIEKDIKEMNLDAIVVASCSPKLHTHTFRGVAERAGINPYNYVQVSVREQ
                     CSWAHSDKPMDATIKAYGLIRAGINRVSHSEALEIIEIMAQKAVAVIGAGVSGLRAAI
                     DLARMGNQVYLIEKEAEVGGKVLTSGKLFMTGENGKEVIDRLYQRIKEMNNINIFTNA
                     NLENISGSFGNFMIDVNVAGELLKLNVGSVLVTTGFDFYQPKEGEYGYQLSDKVITLP
                     QFNKLINSNSGKLTYNGKTIKTIAYIYCVGNRQSKGENKY"
BASE COUNT          467 a          273 c          333 g          377 t
ORIGIN      
        1 ataagggcat ttggcaaggg ctacgaggag ttcttccagc aatcaaaagg aatgggggtc
       61 aactttatta aagggaaagt tgccaaaatc aaccaaaagg atgacaatag tggcgattta
      121 attctccgat acgaggatgt tacaaccgga gccctcaagg aggctaatca cgatttggtt
      181 gtgctttccg ttggggtttt accaaacaac ggaataacaa aagtttttac tgatgataaa
      241 ttagagctgg ataatcaggg ctttataaaa tcgattgacg aacttgtaag cccctcgtta
      301 accagtatcg atggtgtatt tgttgcaggt accgctgcgg gtccaaagga tattcccgat
      361 tcaatactat ctgcaggatc agcggcatcg gaggcagcaa gctatatcaa cagcacacta
      421 taagctgtca tactgagcaa ggcgaagaat caataaatac ttcacctcgc tcattactac
      481 taacaaacta tacattgagc atgatgaaac aacgaatagg agtatatata tgccattgcg
      541 ggggaaacat ctctgactat gttgatgtgg aacagcttgg aaagttgatg gcaaaagagg
      601 atggagtaat tatatcaaag gatgtggttt ttgcctgctc agattctact caaaaagata
      661 ttgagaaaga tattaaagaa atgaatctcg atgccattgt ggttgcatcg tgctcgccaa
      721 aacttcatac gcatacattc cgcggggttg cagagcgtgc tggaattaat ccttacaact
      781 acgttcaggt aagcgtgcgc gagcaatgct cgtgggctca ttccgataaa ccaatggatg
      841 caaccataaa agcctacggc ctaattcgtg caggcataaa tagagtatcg cactccgagg
      901 ctttggaaat catcgaaatt atggcacaaa aggctgttgc tgtaattggc gcaggagtgt
      961 cgggtttacg ggctgcaatt gacctagcta gaatgggcaa ccaggtttac ttaattgaga
     1021 aggaagctga ggttggcgga aaagtattaa cctctggcaa gctatttatg actggagaaa
     1081 atggtaaaga ggttatcgac cggctatacc aacgcataaa ggaaatgaac aatatcaaca
     1141 tattcacaaa tgctaacctt gaaaatattt cgggaagctt tggtaatttt atgattgatg
     1201 tgaatgttgc tggcgaattg ctcaagctca acgttggctc agttctagta acaaccggtt
     1261 tcgattttta tcaacccaaa gagggtgaat atggatatca gctatccgac aaggttataa
     1321 cacttcctca atttaacaaa cttatcaatt ccaattctgg gaagcttact tacaatggca
     1381 aaaccattaa aactatagcc tacatttact gcgttggaaa ccgccagagc aaaggtgaaa
     1441 ataagtactg
//
LOCUS       DAOOQX010000571         1446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_118667_length_1446_cov_2.158160, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000571 DAOOQX010000000
VERSION     DAOOQX010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118667_length_1446_cov_2.158160"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLX53_08480"
     CDS             <1..310
                     /locus_tag="PLX53_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842201.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOY73155.1"
                     /translation="FGGRLLNTRTVYHGVATPINICSPPHYIFQGIPSPFEGGRYHSW
                     IVDRETLPDGLKIEATDSSNQIMALSSKNFDVCGVQFHPESILTPMGKQVVFNWLGQF
                     "
     gene            388..1383
                     /gene="trpD"
                     /locus_tag="PLX53_08485"
     CDS             388..1383
                     /gene="trpD"
                     /locus_tag="PLX53_08485"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOY73156.1"
                     /translation="MELKSILNKLLESQRLSHLEARDLMHGIAGGAVNEAQMSAVLTA
                     YVMRSISLDELIGFREALLELSQEVKLLNGEVIDLCGTGGDGKNTFNISTASTFVVAG
                     ANVPVAKHGNYSASSITGSSNIMEYLGYSFSSNPDKLKREIEQTNVCFLHAPLFHPAL
                     KAIGPVRRQLGVKTFFNMLGPLINPARPKYQLSGVFNAEVGRMFSYLFNRCLTDYRVV
                     YSLDGYDEISLTSDVKVFSKMGDECFSPESIGFSRLSPSCAAGGATVEESAGIFLSVL
                     GNQADKGKISIIIANSALAISCFKGISITEAAHQAQESIESGRAMEQLKRLLAMQ"
BASE COUNT          380 a          325 c          373 g          368 t
ORIGIN      
        1 ttttggaggt aggctgctaa acacccgaac cgtttaccac ggtgtggcaa ccccaatcaa
       61 catttgctcc ccaccccact acatttttca gggcattccc agtccattcg agggtgggcg
      121 ctaccattcg tggattgttg acagggaaac tcttcctgac ggtctcaaga tagaggcaac
      181 cgacagcagc aaccaaatca tggcgctaag ctctaagaat tttgatgttt gtggcgttca
      241 gtttcacccc gagtccattc ttacccccat gggtaagcag gtagtgttca actggctagg
      301 tcagttttaa aataggcagc caagggtgct ctgaaaatta ttgtttcagc tttttaaagc
      361 gcagctaaac agaaaagtat cagatttatg gagttgaaaa gtattcttaa caagttgctg
      421 gagtcgcaac gattgagcca tttagaggca agagacctta tgcatggcat tgccggtggc
      481 gcggtgaatg aggcgcaaat gtcagctgtg cttacggcgt atgttatgcg gagcattagc
      541 ctggatgaac ttataggctt ccgggaggcg ctgcttgagc tttcgcagga ggtaaagcta
      601 cttaacgggg aggtaattga cctttgcggc actggtggtg atgggaaaaa cactttcaac
      661 atttccactg catcaacttt tgttgtagcc ggagccaacg ttcctgttgc caagcatggc
      721 aactatagcg cttcgtcaat aaccggatcg tccaatataa tggaatacct cggctatagt
      781 tttagcagca accctgataa gctgaagcgc gaaattgagc aaaccaatgt ttgcttcctg
      841 catgcccccc tgtttcatcc tgcgcttaag gctattggcc ctgtcaggcg gcagcttggc
      901 gtgaaaacct tttttaacat gcttgggcca ttaataaacc ccgctcgtcc taaataccag
      961 cttagcggag tgtttaatgc cgaggtcggc aggatgttca gctacctttt caaccgatgc
     1021 ctgaccgact acagggtggt gtatagtctc gacgggtacg acgaaatatc cctcaccagc
     1081 gatgtgaaag tgttttctaa gatgggcgat gagtgttttt cgcccgaaag tattgggttt
     1141 agcaggctaa gcccttcgtg tgctgctgga ggagccactg tggaggaatc ggccggcata
     1201 ttcctttcgg tgctgggaaa ccaagccgat aagggtaaaa ttagcataat cattgccaac
     1261 tcggctctag ccattagctg ttttaagggg atttcaatta ccgaggcagc ccatcaggca
     1321 caggaatcaa tagagtcggg tagagcaatg gagcagttaa aacgattact ggcaatgcag
     1381 tgaaccatga acagactaga cgaaattgta gcatgtaaaa aacgtgaggt agccgaaaaa
     1441 aaggag
//
LOCUS       DAOOQX010000572         1445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_118734_length_1445_cov_3.030216, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000572 DAOOQX010000000
VERSION     DAOOQX010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118734_length_1445_cov_3.030216"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="PLX53_08490"
     CDS             complement(<1..862)
                     /locus_tag="PLX53_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOY73157.1"
                     /translation="MDFISSHTGELAALLTAVFWTVTSLAFESASKTVGSLSVNFIRL
                     VLAFIFLSLYSLFARGLLLPTDAGGHQWLWLSLSGLVGFVMGDLFLFQSYVLVGARIS
                     MLIMSLSPPIAAGIGWLSMGETLTLKQALGMLLIFIGIAMVVLRSEKTPNGSKITGRK
                     IKFGYSIPGLLLAFGGAVGQAGGLVLSKYGMAGYDVVASVQIRVITGIVGFAAVLLFM
                     HKSGNLLVAVKNAGAMRRITVGSFFGPFLGVSFSLLAVRYTSTGVASALMSIVPVLII
                     APSAVLLKEKI"
     gene            1064..>1445
                     /locus_tag="PLX53_08495"
     CDS             1064..>1445
                     /locus_tag="PLX53_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOY73158.1"
                     /translation="MNKLVKYIIIVFVVAAVAFLLWYFSSIVAYILISAVLSLMGKPI
                     VDGVCRLKIRNWHPPKAVGATCALIVIWVLFVAFFSFMIPLVVSQFNSLSGVDVGTLV
                     NRFAHPIAGLQEFIQNYLPASAKEF"
BASE COUNT          401 a          352 c          319 g          373 t
ORIGIN      
        1 ttattttctc tttaaggaga accgccgatg gtgcaataat aagcaccggg actatggaca
       61 ttagggcaga ggctacaccg gtgctggtgt agcgaacagc tagcagcgag aaagatactc
      121 ccagaaatgg cccaaagaac gatccaaccg ttatccgacg catagccccg gcgtttttta
      181 cggctacaag cagattgcca gacttatgca tgaagagcag cactgcggca aagcctacta
      241 ttccggtgat aacccttatt tgaaccgagg caactacgtc gtaacccgcc attccatact
      301 tgctgagaac cagtccgcca gcctggccaa ctgcaccacc aaatgccagt aacagcccgg
      361 ggattgaata cccgaacttg attttacgac cggtaatctt actgccgtta ggggtctttt
      421 cgcttctaag aactaccatt gcaatgccaa tgaaaatcag cagcatgccc agggcttgct
      481 ttagggtgag cgtttcgccc attgatagcc agcctattcc cgcagcaatt ggcggtgaca
      541 acgacatgat aagcatcgaa atacgagcac ctaccagcac gtatgactga aacaggaata
      601 gatcacccat aacaaagccc accaagcccg aaagcgatag ccatagccac tgatgccctc
      661 cggcatcagt aggcaatagt aaacctctgg caaataatga gtaaaggctt aaaaaaatga
      721 aggccagaac cagcctgata aaatttaccg atagggagcc tactgttttg cttgccgatt
      781 caaaggccaa cgaggttact gtccaaaaaa ctgcggttag caatgcggcc aactcgcccg
      841 tgtgcgaact tatgaaatcc acttttttta aatttgcttc cccgttggag aacaatttcc
      901 ctgaatagct taatcggtcg agaaaattta tttcgtcatg ctaaaaatca gcccaacaaa
      961 ccaggttgac ctgttggtat gctaaaattt attcttatac ttgcactatc ctttccagaa
     1021 gatacggtta gctgtaagca gcatttgtag aaaatttcat gcgatgaaca agcttgttaa
     1081 gtacatcatt atcgtttttg tagttgcagc ggtagccttt cttctctggt atttctcatc
     1141 tattgtagcc tacattctga tctcagcagt gctgagctta atgggtaaac ccattgtaga
     1201 tggggtttgt aggctgaaaa ttaggaactg gcatccaccc aaggcagtag gcgctacctg
     1261 cgcgcttatt gttatctggg tgctgtttgt ggcatttttt agctttatga tacctctggt
     1321 ggtttcgcag tttaactccc ttagcggagt tgacgttggc acactagtca ataggtttgc
     1381 ccatccaatt gccggactgc aggaattcat ccaaaactac ctaccagcta gcgccaagga
     1441 gtttt
//
LOCUS       DAOOQX010000573         1439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_119584_length_1439_cov_2.986272, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000573 DAOOQX010000000
VERSION     DAOOQX010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119584_length_1439_cov_2.986272"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>257)
                     /locus_tag="PLX53_08500"
     CDS             complement(<1..>257)
                     /locus_tag="PLX53_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HOY73159.1"
                     /translation="YEMNFCQEFYTEGVSIQPARVFNRLYSNSPTPFGAFLKIGEKFL
                     MAASPERYLRKNGNVVVSQPIKGTAPRMASPDMDSQVVEKL"
     assembly_gap    258..267
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<268..819)
                     /locus_tag="PLX53_08505"
     CDS             complement(<268..819)
                     /locus_tag="PLX53_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73160.1"
                     /translation="MRKRLTYLVENPEVVTDNMAKALLSWDVGIVLRGKDAMPVPGTD
                     MVSQFDCAAGFGVAASCKPADNFFKNFEYFTDANNDWILGFLGYDLKNELENLQSGNF
                     DGLAFPEMFFFVPEVFMSIADHNLCVYYYPGKTSDLTVSKLVDDLLRVELAANPQQPT
                     ITFKSRYSRYEYLTQVQRIKNHIG"
     gene            complement(914..>1439)
                     /locus_tag="PLX53_08510"
     CDS             complement(914..>1439)
                     /locus_tag="PLX53_08510"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOY73161.1"
                     /translation="VVFFFYPADFTFVCPTELLAFQDKLEEFEKRNTQVVACSVDSEF
                     SHWKWLQTEQKDGGIKGVTYPIVSDLSKTISENFDVLAGHYDYDDNNGDAVFVGVPQA
                     FRGLFLIDKSGVVRHQVVNDMPLGRNVDEVIRMVDALQYFEEHGEVCPANWKKGEKAM
                     KATQDGVSDYLSHR"
BASE COUNT          403 a          348 c          297 g          381 t
ORIGIN      
        1 ttagcttttc caccacctgc gaatccatgt ctggggaagc cattcttggc gcagtgcctt
       61 taattggctg gcttaccact acattcccgt ttttcctaag gtaacgttcg ggagaagctg
      121 ccattaaaaa cttttcgcca atttttaaaa atgcgccaaa cggtgtaggt gagttgctat
      181 ataagcggtt aaacacccgg gcaggctgaa ttgatacccc ttcggtgtaa aactcctggc
      241 aaaaattcat ctcgtaannn nnnnnnngcc gatatggttc ttaatgcgct gaacctgggt
      301 aaggtattca tatctcgagt atctggactt aaaagtgatg gtaggctgtt gagggttagc
      361 agccaattca actcttagca ggtcgtcaac aagcttgcta acagttaggt cagaggtttt
      421 accgggatag tagtacacgc aaagattgtg gtcagcaata ctcataaata cttccggaac
      481 gaagaagaac atttcgggaa atgccagccc atcgaaattc cccgattgaa gattctccag
      541 ctcattttta aggtcgtaac ctaaaaaacc taaaatccaa tcgttattag catcggtaaa
      601 gtattcgaag tttttaaaaa aattatccgc aggtttgcag ctggcagcca caccaaatcc
      661 tgctgcacaa tcgaactggc ttaccatgtc tgttcccgga accggcatag catcctttcc
      721 tcttaaaaca atgccaacat cccagcttag cagggcttta gccatgttgt cggtaaccac
      781 ttcagggttt tcgacaaggt atgttaaccg tttcctcatg ccacaaaatt agcattaaag
      841 atgaaggatt tagcacaaaa aaaccgcccc tagaggcggt tcagcaagtt aagtggatat
      901 gcaagtaggc ttactaccta tggcttaggt agtccgaaac gccgtcctgg gttgctttca
      961 tggccttttc accttttttc cagttggcag ggcaaacctc gccatgctcc tcgaaatact
     1021 gaagtgcgtc aaccatacgg attacctcgt ccacattcct tcccagaggc atatcgttaa
     1081 ccacctgatg gcggacaacc ccggacttgt caatcagaaa caatcccctg aatgcctgtg
     1141 gaacgccaac aaatacagca tctccgttat tgtcatcgta gtcgtaatga ccggccagca
     1201 catcaaaatt ctcggagatt gttttggata aatccgaaac aattgggtag gtaaccccct
     1261 taatgccgcc atctttttgt tcggtttgca gccatttcca gtgcgaaaac tccgaatcaa
     1321 ccgagcatgc aaccacctgt gtgtttcttt tttcaaactc ctccagctta tcctgaaatg
     1381 ctaaaagttc tgttgggcaa acaaaagtaa aatcggcagg gtagaaaaag aacaccaca
//
LOCUS       DAOOQX010000574         1437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_119932_length_1437_cov_1.922576, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000574 DAOOQX010000000
VERSION     DAOOQX010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119932_length_1437_cov_1.922576"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLX53_08515"
     CDS             <1..373
                     /locus_tag="PLX53_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOY73162.1"
                     /translation="MLKQGYGAIVNTSSDAGLIALPGLNPAYVAAKHGIVGLTKIAAI
                     EFADRNIRVNAIAPGMTHTGMTHDLIAGAPSEREVASYLPIKRVADPAEIAEAAIWLL
                     SGKASFVTGAILSVDGGSVAD"
     gene            428..1021
                     /locus_tag="PLX53_08520"
     CDS             428..1021
                     /locus_tag="PLX53_08520"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOY73163.1"
                     /translation="MKKLFLLLGLASALTFTVGAQTSAWVIDNMHSNFGFKISHMGIS
                     LVEGEFNEFSGTITTSREDFSDAKAEILVKAASINTDVQPRDQHLRSADFFEVEKYPD
                     ITFVSTKMERTYGNNFKLTGNLTMHGITKTIVLDVTYNGQAYEEQMKVQKAGFTVQGV
                     LNRDDFGISAFSFPLPSGAPMVGREVFLTIPLELIKQ"
     gene            1099..>1437
                     /locus_tag="PLX53_08525"
     CDS             1099..>1437
                     /locus_tag="PLX53_08525"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOY73164.1"
                     /translation="MKAARLHELGKPLVVEEVSIPELNDDDVLVEVKATFVAPSMKDI
                     SHLGGNFIRPTLPAILGSDAVGVISKLGRRVKRLRVGQYVWVNSMLYYNTDEFALRGQ
                     EGLSDSMAFQG"
BASE COUNT          396 a          299 c          324 g          418 t
ORIGIN      
        1 aatgctcaaa cagggctacg gtgctattgt gaatacctca tccgatgcgg gattaattgc
       61 cctaccgggt ctcaaccccg cttacgttgc agctaagcac ggcattgttg ggctaacaaa
      121 aattgccgcc attgagttcg ccgataggaa tattcgagtt aacgccattg caccagggat
      181 gacacatacc ggaatgactc atgacttaat tgccggagcc cccagcgaaa gggaagtagc
      241 ttcatacctc ccgattaaaa gggttgccga ccctgccgaa attgccgagg ccgcaatttg
      301 gttgctttcc ggcaaagcct cgtttgtcac aggagctata ctatcagttg atggcggctc
      361 agtggccgac tagatggcgc ttatgtaatt ttaaccttac tgatcattaa ccttaaaact
      421 atttgctatg aaaaaattat ttttgctact tggtttggct tcagccctaa cttttactgt
      481 tggggcacag acttctgcat gggtaataga taacatgcac tccaattttg gctttaaaat
      541 atcccatatg ggtatatcac tggttgaggg cgagttcaac gaattttcgg gaactatcac
      601 tacctcaagg gaggattttt cagatgcaaa agctgaaata ctggttaaag ctgcaagcat
      661 caatactgat gttcagccta gagaccagca cctaaggtca gcagatttct tcgaagttga
      721 aaaatatccc gatatcacgt ttgtgagtac aaagatggaa cgcacctatg gtaataattt
      781 caagctcact ggtaatctaa ccatgcacgg gattaccaaa accattgttc tcgatgttac
      841 atacaatggt caggcttatg aggaacaaat gaaggtacaa aaagccggat ttacggttca
      901 aggagtgtta aaccgtgacg attttgggat ttcagcattt agctttccat taccttctgg
      961 cgccccaatg gttgggcgtg aagtatttct cactattcca cttgagctaa tcaaacagta
     1021 atgttataat tctttaatga ttgcccttat atcattcact attcttatca cttaatatga
     1081 atttctataa attatgctat gaaagctgca cgtttgcatg agctgggcaa accccttgtg
     1141 gttgaagaag tgtccattcc tgaacttaat gacgatgacg ttctggttga agttaaggcg
     1201 acctttgttg ctccttctat gaaggatatt tcacacctag gaggaaattt cattcgtcca
     1261 accctccctg ctattctcgg atcggatgct gtgggtgtta tctcaaagct tggtagaagg
     1321 gtaaagaggc tgagagttgg acagtatgtt tgggtgaatt caatgctata ctacaatacc
     1381 gatgagtttg ctttaagggg acaggaaggg ttgagcgatt ccatggcatt tcaggga
//
LOCUS       DAOOQX010000575         1435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_120168_length_1435_cov_2.318841, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000575 DAOOQX010000000
VERSION     DAOOQX010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120168_length_1435_cov_2.318841"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLX53_08530"
     CDS             complement(<1..611)
                     /locus_tag="PLX53_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73165.1"
                     /translation="MLVNSHLKNGFLLLAGLLVSNAAIAQENLEADSIFTWKLVELTN
                     PSSHSCIQFIPRVDITPVSWDFGDGSPASADVSPVHTYTYVSWSDSVEVTLTYTAGGA
                     NTVKTRQIPLSPAYFHCIHDSNLGQAASFKYIFRNAYMIDNNPGNLGNLHFSWTIDSE
                     PLAENTFSNPDLGQWPNIYYTFLNGGTHQVKLEVYNTTNATEVA"
     gene            complement(731..>1435)
                     /locus_tag="PLX53_08535"
     CDS             complement(731..>1435)
                     /locus_tag="PLX53_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOY73166.1"
                     /translation="TSKISTFFTTSMIANLFSSTANFVVNLGIALFAITFITFFFLKD
                     DTLFYNGVILLFPSRFEQQLSHALSSINRLLRRYFIGLLIESTLIMLLDTLWLVLIGL
                     HFQTAIVIGLIIGILNVIPYVGPLIGIIIGTLIGLASQLQLISATELLPLTLWMVGAM
                     LATKLIDDAVFQPVIYGNSVKSHPLEIFLVILIAGSLAGVLGMLLAIPAYTVLRVFAK
                     EFFNNFRLVQKITEKI"
BASE COUNT          435 a          282 c          351 g          367 t
ORIGIN      
        1 gcaacttctg tagcattagt agtattgtaa acttcgagtt taacctggtg ggttcctccg
       61 tttaaaaagg tgtagtaaat atttggccat tgccccaggt ccggattgct aaaggtattc
      121 tcggcaaggg gttctgaatc aatggtccat gagaaatgaa gatttccaag gttacctggg
      181 ttgttatcaa tcatgtatgc attcctaaaa atatacttaa aggaggccgc ttgtcccaga
      241 ttggagtcat gaatacaatg aaagtaggcc gggcttaagg gtatctgacg ggttttaacc
      301 gtattggctc cgcctgcggt ataggtaagc gtaacctcaa cgctgtcgct ccagctaacg
      361 taggtgtaag tatgaacagg gctaacgtcg gctgaggctg gacttccatc gccgaagtcc
      421 cagctaaccg gagtaatatc gactcgggga ataaattgaa tgcagctatg cgagctgggg
      481 ttggtaagct caaccagctt ccaggtaaaa atggagtcag cttcaaggtt ctcctgggca
      541 attgctgcat tcgataccaa aagacctgcc agcagcaaaa agccgttttt tagatgagaa
      601 tttacgagca tgaggttttc tgttaaattt tcctgataaa tctatagtga gtgaagcgtt
      661 tatcaactag taagttacaa acatatagtt gttttatagc agtaaaaata cacttttaca
      721 tcagtaaacg ctaaattttc tctgtaattt tttgaaccag cctgaagtta ttaaaaaact
      781 ccttggcaaa aaccctaagt accgtgtagg caggaattgc cagaagcatt cccagaacgc
      841 cggcaaggct gcccgcaata agaattacca ggaaaatttc gaggggatga gatttgacgc
      901 tgttcccata aatcacaggt tgaaaaaccg catcgtcgat tagcttggtg gcaagcatgg
      961 ctccaaccat ccaaagagta aggggcagca attcggtagc cgaaatcagc tgcagctggg
     1021 aagccaaccc tattagcgtt ccaattataa tgccgataag ggggccaaca taaggaataa
     1081 cgtttagaat gccgataatc agcccaatca ctattgccgt ttgaaaatga agaccaatga
     1141 gtacaagcca gagggtatca agcagcatga tcagggtgct ctctattagg agcccaataa
     1201 agtagcgtcg gagcagcctg tttatgcttg ataaagcatg cgacagctgc tgttcaaatc
     1261 ttgacgggaa aagcaggatt accccgttgt aaaaaagggt atcgtccttt aggaagaaga
     1321 aggtaatgaa tgttatggca aaaagagcga tgcccaggtt aacgacgaag ttcgccgtag
     1381 agctgaacag gttggcaatc atggatgtag taaaaaaggt gctgattttt gatgt
//
LOCUS       DAOOQX010000576         1433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_120394_length_1433_cov_2.757620, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000576 DAOOQX010000000
VERSION     DAOOQX010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120394_length_1433_cov_2.757620"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLX53_08540"
     CDS             complement(<1..425)
                     /locus_tag="PLX53_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOY73167.1"
                     /translation="MMIGYGGLPEAFTNYSTSKVAVLPVAYDGTSTWVKGADKGPYAL
                     LEASANMEVYDIETNSEVYRIGIFTDEIMEGDFSSPDQMVSAVRSRTLKLINDGKLVA
                     TIGGEHSVSIGAVQAHARTYDHLTVLQIDAHADMRSSYEG"
     gene            complement(605..1117)
                     /locus_tag="PLX53_08545"
     CDS             complement(605..1117)
                     /locus_tag="PLX53_08545"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOY73168.1"
                     /translation="MKRLSILLLGAIMAAGFTANAQNCGKGMGMGMGAPKMNPDSLQS
                     CNIPNLTPDQQKKIYDLRVAHIKAVTPLRNQLAEKYAHLKTLESAEKPDLNAINKTID
                     EIVSIKGQLMKSRAAHRADVSALLTDEQKVFFNAHQGMGNRMHQKGMGAGMHQGMGKN
                     KPMGSCQQMN"
     gene            complement(1142..>1433)
                     /locus_tag="PLX53_08550"
     CDS             complement(1142..>1433)
                     /locus_tag="PLX53_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73169.1"
                     /translation="IRLVSGEINNEITRAEVDTLKLNMLIDRYASLQRQLKELSVRHL
                     MEVRALCNPGQQQQFCRMARQINRRHQQMNRNNSNDRMRGMPPRGHQNQNNF"
BASE COUNT          350 a          334 c          308 g          441 t
ORIGIN      
        1 ccctcatagc tgctgcgcat atcggcatgt gcatcaattt gaagaacggt gaggtggtca
       61 taggtgcggg catgagcctg aacagcacca atgctaacag aatgctcacc ccctatggtt
      121 gcaacaagct taccgtcgtt gattaatttt agtgttcggc tgcgaaccgc actaaccatt
      181 tgatcggggc tgctgaaatc gccctccatg atttcatctg taaaaatgcc tatcctatat
      241 acctcggagt tggtttcaat gtcgtaaacc tccatgtttg cggaagcttc gagaagtgca
      301 tagggtccct tgtcggcgcc ctttacccat gtgctggtgc catcgtaagc cacaggtaga
      361 acggctacct ttgatgtact gtagtttgta aatgcctctg gtagtccgcc gtatccaatc
      421 atcataaaac cttaaaaaac tttgcaaaat tactaaattt ttggagaatt taacattgaa
      481 tttttcatga taatggccaa taaccaactg cccgaaacag ctagggtttt catcatacaa
      541 aaaaactggc ctccccgctt gggagaagcc agcattttac cttcaattct tatttttaat
      601 tggcttagtt catttgttgg catgaaccca tgggtttatt ctttcccatt ccctgatgca
      661 tcccagcacc catgcccttt tgatgcattc tattacccat cccctgatgg gcgttaaaga
      721 aaactttttg ctcatcggta agtaacgctg aaacatcggc cctatgagca gcacgactct
      781 tcataagttg ccctttaatg ctaacaattt catcaatagt tttgttgatg gcattcaaat
      841 caggcttttc ggccgattcc agtgttttca gatgggcata cttctcagcc agctgattac
      901 gaagtggggt aacggctttg atgtgggcta cccgaagatc gtaaatcttt ttttgttgat
      961 cgggagtaag gttagggatg ttgcagcttt gtaggctatc aggattcatt ttgggtgctc
     1021 ccattcccat gcccataccc ttaccgcaat tctgcgcatt ggctgtaaat ccggctgcca
     1081 taatggcacc aagcagtaaa atactcaatc gtttcatagt tttgcaagtt taagttaaaa
     1141 tttagaaatt attctgattt tggtggcccc tggggggcat accacgcatg cgatcattac
     1201 tgttatttcg gttcatttgt tggtgacgcc ggttgatttg acgtgccatc cggcagaact
     1261 gttgttgttg accagggttg caaagtgccc ttacttccat tagatgtctt accgatagct
     1321 ccttaagctg tcgttgcagg ctggcatacc tgtcaatgag catattcagc ttaagggtat
     1381 caacttcggc ccgggtaatt tcgttattaa tttcacccga aaccagccga atc
//
LOCUS       DAOOQX010000577         1428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_121114_length_1428_cov_2.286235, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000577 DAOOQX010000000
VERSION     DAOOQX010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121114_length_1428_cov_2.286235"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLX53_08555"
     CDS             complement(<1..238)
                     /locus_tag="PLX53_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOY73170.1"
                     /translation="MALILLLDNYDSFTYNLLHYVREFGRHKVEVFRNDQLMVEDVNK
                     YDGIIVSPGPGLPSEAGNMPAIVKTYAKSKRIFGV"
     gene            complement(279..>1428)
                     /locus_tag="PLX53_08560"
     CDS             complement(279..>1428)
                     /locus_tag="PLX53_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOY73171.1"
                     /translation="IIAFVDSFIKSFTLEGSNIHPETAGFYGFTAYDAVNFFDSVNIN
                     TLDDSIPLLRYDFYQVILAFNHFNNTLTLVEYTAPGMNAIGDRVLSLLANRNAPVFPF
                     VAAAEEESNMSDEQFMAMVEQGKLHCARGNVFQIVLARQFSRGFSGDDFNVYRALRSI
                     NPSPYLFYLDYLNYRIFGSSPEAHLKVNSGIATINPIAGTAVRTGNADMDKALAANLL
                     QNPKENSEHCMLVDLARNDLSRYAHNVTVEKYREIYTYSHVVHMVSNVRGTLSTSYSP
                     YALMASTFPAGTLSGAPKYKAIQLISEIEPGPRGIFGGAVGFIGLDGNLNHAIIIRSF
                     LSKMGVLKYQAGAGVVNGSTPEGELAEVNSKVTALRMAVDKASVNFSN"
BASE COUNT          403 a          336 c          305 g          384 t
ORIGIN      
        1 aaactccaaa aattcgcttc gactttgcgt aggttttcac tatagccggc atgtttccag
       61 cttcggaggg caagcccggc cccggcgata caattattcc atcatacttg ttaacatcct
      121 caaccatcag ctggtcgttg cggaaaacct caaccttatg cctcccgaat tcccggacat
      181 agtgaagcag gttgtaggta aacgaatcgt agttgtcgag tagtagaatt agcgccatag
      241 tcaaaattga ttaaattact tttgggtttt cacgcatttt aatttgaaaa atttactgaa
      301 gccttatcaa cagccattct gagggcagta acttttgagt taacctcggc cagctcgcct
      361 tcgggggtgg agccattaac aacacctgcc ccggcttgat acttaagaac tcccatcttg
      421 ctaagaaaag aacgaatgat gattgcatgg ttgaggttgc catctaatcc gataaacccc
      481 acagcaccac caaatattcc acgcgggccg ggttcaatct cgctaattaa ctgaatagcc
      541 ttgtacttgg gtgctccact tagcgtccct gcaggaaagg ttgaagccat caaagcgtaa
      601 ggactgtagc tggtcgacaa tgttcccctg acatttgaaa ccatatgaac cacatgagag
      661 taggtatata tttccctgta cttttcaaca gtaacgttgt gcgcatacct gctcaggtca
      721 tttcttgcca ggtcaaccaa catgcaatgc tccgaattct cctttgggtt ttgtagcaaa
      781 ttggcagcaa gtgctttatc catatctgca ttacctgtcc ttactgccgt accggcaata
      841 gggttaatgg ttgcaatgcc gctatttacc tttaggtgag cctctggtga cgagccaaaa
      901 attctatagt taaggtagtc gaggtagaaa aggtaggggg aaggatttat ggagcgtagc
      961 gccctgtata cattaaaatc gtctccacta aaacccctgc tgaattgtct agccagcact
     1021 atttgaaaca cgttcccacg tgcacaatgt aattttcctt gctcaaccat agccataaac
     1081 tgctcatcgc tcatattcga ctcttcctca gcggcggcca caaatgggaa aacaggcgca
     1141 ttcctgttgg ccagaagcga aagcaccctg tctcctatag cattcatccc cggtgcagta
     1201 tattcaacaa gcgtcagggt gttgttaaaa tggttgaaag ctagaataac ctggtagaaa
     1261 tcatacctta acaagggaat tgaatcgtca agggtattaa tattgacgct gtcaaaaaag
     1321 ttgacggcat cataggccgt aaacccataa aatccggctg tttccggatg gatgtttgaa
     1381 ccctcaagag taaaactttt aataaaggag tcaacgaaag cgataatt
//
LOCUS       DAOOQX010000578         1427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_121205_length_1427_cov_3.063411, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000578 DAOOQX010000000
VERSION     DAOOQX010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121205_length_1427_cov_3.063411"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..522
                     /locus_tag="PLX53_08565"
     CDS             121..522
                     /locus_tag="PLX53_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOY73172.1"
                     /translation="MTVEDFNSCVDLYSDGIYRFALKMLGDTMLAEDNVQDSFERLWV
                     KRGEIDRSKAKSYLFTTSYHASVDAIRRDRHKQELENAQPVLEIGYQPNFDVKEIINH
                     AVDQLPTVQKSVLLLRDYEGYSYGEIAGKQD"
     gene            611..>1427
                     /locus_tag="PLX53_08570"
     CDS             611..>1427
                     /locus_tag="PLX53_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73173.1"
                     /translation="MSINLNTYEEYLLDYIDGKLCPEVEAELVKFLRLHPDIASELDG
                     LNQLTIPKPNLSFSGKQRLRRTVEQDLFSCNEMDYLCIAELEGDISEVEKARLEELKG
                     QHPEVERFRNLYAHTLLLSENSHTFPNKASLKRHSIISKRRIMLTAAAAITAAIVGLH
                     TIHKLSLPDDLQPQNQMAKSAIIESQPREYRPTSTTTYPQTQPKPESRVAVSLAYKTS
                     TSTISMDNEELQPMRTNVAELKQIASKEPIIEPIASITPKPFPITGKVQGVAFR"
BASE COUNT          445 a          338 c          310 g          334 t
ORIGIN      
        1 taaagggcga taaattgcgg tataactttt gaacaacttg tcctgctcca tttaaaggat
       61 accttgagaa gtgtaacttt taagcaactc catcgtctta catgaaattt acagccatag
      121 atgaccgttg aggattttaa cagctgcgta gatttatact ccgacggaat atatcgcttt
      181 gcattgaaaa tgcttggcga caccatgctg gcagaggata acgtgcagga ttctttcgag
      241 aggctatggg ttaagcgtgg tgaaattgat aggtcaaaag caaagtcata ccttttcacc
      301 acttcgtacc atgcctcggt tgacgccatc aggagggata ggcacaagca ggagctagaa
      361 aatgcgcagc cagtattaga aattggctat caacccaatt ttgacgtaaa ggaaattatc
      421 aaccatgcgg tggaccagct tccaacggtt caaaaatccg tgctgctgct ccgcgactac
      481 gaggggtact cctacggtga aattgcaggg aaacaggact gaacgaatcg caggttaagg
      541 tttacatcta cagagccaga gttttcctaa aggaaaaact cgtaagtatt gaaaatttaa
      601 tttaactccg atgagcatca acctaaatac atacgaggag tacctgctcg actacatcga
      661 tggcaagctt tgcccggagg ttgaggctga actagtaaag tttcttcgct tacatcccga
      721 cattgcttca gaacttgatg gattaaacca gctaaccatt ccaaaaccaa acctttcctt
      781 ttcagggaaa caacggctta ggagaaccgt tgaacaggat ctcttctcat gcaacgagat
      841 ggactacctt tgcatcgccg aactggaggg tgatatttcc gaggttgaaa aagcaaggct
      901 cgaagagctg aaagggcagc atcccgaggt tgaacggttc cgcaaccttt atgcccatac
      961 ccttttactt tccgagaata gccatacttt tccaaacaaa gcttcgctaa aaaggcatag
     1021 tatcatttca aaacgaagga ttatgctaac agcggcagca gcgattactg cagccattgt
     1081 tggccttcat accatccaca agctaagcct tcccgacgat ttgcaacccc aaaatcaaat
     1141 ggccaaaagc gcaatcattg aatcgcagcc aagggagtac aggccaacct ccaccaccac
     1201 ttacccacaa acccagccaa aaccggagag ccgggtagca gtgtcgctgg catataaaac
     1261 ctctacatca acaataagca tggacaacga agagcttcag cccatgagaa caaatgtggc
     1321 agagctaaag caaatagcct ccaaagagcc gataattgag ccaattgcta gtattacccc
     1381 taaacccttt ccaataactg gcaaggtaca aggtgttgct tttcggg
//
LOCUS       DAOOQX010000579         1424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_121636_length_1424_cov_2.845142, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000579 DAOOQX010000000
VERSION     DAOOQX010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121636_length_1424_cov_2.845142"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..>1424
                     /locus_tag="PLX53_08575"
     CDS             311..>1424
                     /locus_tag="PLX53_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73174.1"
                     /translation="MNNLKRIFLAGLTLVGSAASGQIYFTEGFENGTRPSGWSEIYVS
                     GSQVWLYRNGGHSPNDPSWSIPASQEDITRNPSSAHSGTYNAIFFKQSSAHEQTKLVT
                     KAIDLSEGIKPELSFWLCQVPWTFGGSTNNDELRVYYKNTFGGAWTLLAEYTDPIFDW
                     TEYKINLPNPSSTYYIAFEGKINWGFGTCIDDIVIEEKGYQERYVSELTVSQADFSFI
                     PSGSPNEPILKVGVKVFGNMGSATLNTLTAKSLNTSDNDIEANGVKLYFTPTGTFSAD
                     NLLVEGKNFTSGQVTFNNINFNLPNGQSYLWLTYDVKQNAVHGNIMDARLDAGSIQVS
                     DSLYPKVNESPEGNRVIYETIYRQDFEGTHQWLLSGE"
BASE COUNT          407 a          339 c          321 g          357 t
ORIGIN      
        1 gcgccccttg ctgcatattc cgccccttcg gggcttaaaa cactagacaa gttccgccgg
       61 tagtcgagca agttccgaaa tccgccggta ggcggacaag ttctggaatc taaaatccgc
      121 cagtagtcgg aaaaattctt gaatcctgct tgattttttt tatcctttat cctttaccct
      181 ttaacctttt tggatatttt atcgtaaaat aatattaatt tgcattagta aactacctct
      241 taccctaact taataatcat ctattaagct gataaataga tacgctaaaa ccataagaaa
      301 atatacccta atgaataacc taaaaagaat atttctggcg ggcttaactc ttgtaggttc
      361 ggctgccagc ggacagatat attttaccga gggatttgaa aacggtacca ggccttctgg
      421 ttggagcgag atttacgttt cgggaagtca ggtatggctg taccgcaatg ggggtcacag
      481 ccctaacgat cccagctgga gcattccagc ctcccaggag gacatcaccc gcaacccttc
      541 ttcggcccac tcgggtacct acaacgccat attcttcaaa caaagttctg ctcacgagca
      601 gaccaagctg gttactaagg caatagacct aagcgagggg ataaaaccag aactcagctt
      661 ctggctttgc caagttcctt ggacttttgg gggtagcacc aacaacgatg agctgagggt
      721 gtactacaag aatacgtttg gtggagcctg gacattgctg gccgaataca ccgatcccat
      781 tttcgattgg acagagtata agattaacct tccaaacccc tcgtcaacct actacatagc
      841 ctttgagggt aaaatcaact ggggctttgg aacctgtatt gacgacattg ttattgagga
      901 aaaaggctac caggaaaggt atgtgagcga gcttactgtt agccaggccg atttttcatt
      961 cattccaagc ggttccccca acgagcccat cttaaaggtt ggggttaagg tttttggtaa
     1021 tatgggtagc gcaacactca acaccctaac ggcaaagtcg ctaaacacca gcgataacga
     1081 tattgaggcc aatggagtaa aactctactt caccccaacc ggaacattta gcgctgacaa
     1141 cttgctggtt gaggggaaaa attttacctc aggccaggtt accttcaaca atatcaactt
     1201 caacctaccc aacgggcagt cgtacctatg gctaacctac gatgtgaagc agaatgctgt
     1261 tcatgggaat ataatggatg ccaggctgga cgccggaagc atacaggtta gcgactcgct
     1321 ttacccaaag gtgaacgaat cgcccgaagg taaccgggtg atatacgaaa caatttaccg
     1381 tcaggacttt gaagggacac accagtggtt gctgagcgga gagt
//
LOCUS       DAOOQX010000580         1423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_121777_length_1423_cov_3.089181, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000580 DAOOQX010000000
VERSION     DAOOQX010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121777_length_1423_cov_3.089181"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..>1423
                     /gene="pckA"
                     /locus_tag="PLX53_08580"
     CDS             81..>1423
                     /gene="pckA"
                     /locus_tag="PLX53_08580"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOY73175.1"
                     /translation="MANLDLSAYGVNQPAEVFYNPSYELLYQHETDPSLQGYERGIVT
                     KLGAVNVDTGVFTGRSPKDKYVVKEPNTEKNVWWAQPGKKSSDNKPITQEVWNHLKDI
                     SAKQLSGKKLYVVDAYSGANEDTRLCVRFVMEVAWQAHFVKNMFIRPTDEELKNFKPD
                     FIVLVASKTSNPKWKEQNLNSEVFVAFHLTERMALIGGTWYGGELKKGIFSVMNYFLP
                     LKGIASMHCSANVDQKNGNVAIFFGLSGTGKTTLSADPQRALIGDDEHGWDDNGIFNL
                     EGGCYAKCIDLDPEKEPDIYAAIRRDALLENLDVDSEGNIDFHSKVKTENTRVSYPIY
                     HIQNIVKPVSKAGHAKHVIFLTADAFGVLPPVSRLSPEQMQYHFLSGFTAKLAGTERG
                     VVEPQPTFSSCFGQAFLMLHPTVYARELVKKMQLHGSKAYLVNTGWIGGPYGVGKR"
BASE COUNT          395 a          333 c          340 g          355 t
ORIGIN      
        1 tgtttaaggg gttacatttt ggccattcgt aattatattg tatgtttggt tcaaaattaa
       61 actataaaaa tccattaacc atggctaatc tggacttatc ggcatacggt gtaaaccaac
      121 ctgccgaagt tttttacaat ccgagttacg agcttcttta tcaacacgaa accgatcctt
      181 cactccaagg atacgagaga ggcattgtaa ccaagttggg cgctgttaat gtggacaccg
      241 gcgtttttac cggacgctca cctaaggata agtatgtggt gaaggagccc aataccgaaa
      301 aaaatgtttg gtgggcccag ccagggaaga aaagttccga caacaagccc atcacccagg
      361 aggtatggaa tcacctgaag gatatcagcg caaaacagct cagtgggaaa aaactctacg
      421 ttgttgatgc ctatagcggt gcaaatgagg acacacgcct ttgcgtcagg tttgtcatgg
      481 aggttgcctg gcaggcacat tttgtaaaga acatgttcat taggccaacc gacgaggaac
      541 ttaaaaattt taaacccgac tttattgttc tggtagcctc caaaacctca aaccctaaat
      601 ggaaggagca gaaccttaac agcgaggttt ttgtagcctt tcatcttacc gagcgcatgg
      661 ccctaattgg tggcacctgg tatggcggag agttgaaaaa gggtattttt tccgtaatga
      721 actatttcct gcccttaaag gggattgcct ccatgcactg cagcgctaac gttgatcaaa
      781 aaaatgggaa tgttgccatt ttctttgggc tttcaggaac aggaaaaaca accctttccg
      841 ccgatcccca acgcgccctt ataggcgatg acgagcatgg gtgggacgac aacggaattt
      901 tcaaccttga gggtggatgc tatgccaaat gcatagacct ggaccccgaa aaggaacccg
      961 acatctatgc cgctattcgt cgggatgcac tgctcgagaa cttggatgtt gacagcgagg
     1021 gaaatattga ctttcactct aaagttaaaa ctgaaaacac ccgggtatcc tatccaattt
     1081 accatatcca aaatattgtt aaaccggttt cgaaggcagg acacgctaaa cacgtaatat
     1141 tcctcaccgc cgatgccttt ggagtgctac cgccggtatc gcggctttcg cccgagcaga
     1201 tgcagtacca ctttctaagt ggttttactg ccaagcttgc aggcacagag aggggagtgg
     1261 ttgagcccca gccaactttc agcagctgtt ttggccaggc cttccttatg ctccatccca
     1321 cggtatatgc ccgtgagctg gttaaaaaaa tgcagcttca cggctcaaaa gcttatctgg
     1381 tcaacaccgg ctggattggc ggaccctacg gggttggtaa acg
//
LOCUS       DAOOQX010000581         1421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_122070_length_1421_cov_2.773792, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000581 DAOOQX010000000
VERSION     DAOOQX010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122070_length_1421_cov_2.773792"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..378)
                     /gene="rpsM"
                     /locus_tag="PLX53_08585"
     CDS             complement(1..378)
                     /gene="rpsM"
                     /locus_tag="PLX53_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOY73176.1"
                     /translation="MARIVGVDLPKNKRGEVALTYIYGIGRSSSRRILDKAGISYDKK
                     VSEWDDNELNAIRTILNEEYKVEGELRSMVQMNIKRLMDIGCYRGIRHRLGLPVRGQS
                     TKNNARTRKGRKKTVANKKKATK"
     gene            complement(422..538)
                     /gene="ykgO"
                     /locus_tag="PLX53_08590"
     CDS             complement(422..538)
                     /gene="ykgO"
                     /locus_tag="PLX53_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOY73177.1"
                     /translation="MKVRTSIKKRSDDCKIVRRKGRLYVINKKNPKFKQRQG"
     gene            complement(558..776)
                     /gene="infA"
                     /locus_tag="PLX53_08595"
     CDS             complement(558..776)
                     /gene="infA"
                     /locus_tag="PLX53_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOY73178.1"
                     /translation="MSKQPAIEKDGTIVEALSNAMFRVELDNGHVIIAHISGKMRMHY
                     IKILPGDKVRVEMSPYDLTKGRITFRYK"
     gene            complement(789..>1421)
                     /gene="map"
                     /locus_tag="PLX53_08600"
     CDS             complement(789..>1421)
                     /gene="map"
                     /locus_tag="PLX53_08600"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOY73179.1"
                     /translation="RDNGAKPGFLGYDGFPATLCVSVNDVVVHGIPSNRVLHEGDIVS
                     VDCGTYMHGFYGDSAYTFEVGDVAPQVKRLLRVTRECLEHGVEAAVEGARIGDVSHAI
                     QTHAESNGYSVVRELVGHGLGRDMHESPEVPNYGNKGRGPKLPAGLVICIEPMINLGT
                     RYVYQDSDGWAVRTQDGKPSAHYELAVVVRNGKAETLSTFQYVEQVITKS"
BASE COUNT          341 a          376 c          278 g          426 t
ORIGIN      
        1 ttatttggtt gctttcttct tgttggcaac agttttttta cggccctttc gagtgcgggc
       61 gttattcttg gttgattggc ctctaacagg caaaccaaga cggtggcgaa ttccacggta
      121 gcaaccaatg tccattaaac gtttaatgtt catctgcacc attgagcgaa gctcgccttc
      181 tactttatac tcttcgttca aaatggttct aatggcgttg agctcattgt catcccattc
      241 ggacaccttc ttatcgtagc taattcccgc cttatcaaga atcctgcgcg agctgcttcg
      301 gcctatcccg taaatgtagg ttagcgcaac ttcaccacgc ttatttttcg gcaaatcaac
      361 accaactatt cgtgccatac ctgatttctt aaaaaatttt tagactgcaa aattaaacaa
      421 tttatccttg acgctgcttg aacttaggat tttttttatt aatcacgtac aagcggcctt
      481 ttcgcctgac tattttacaa tcgtcgctgc gcttcttgat tgaggttctt actttcatct
      541 cctttgataa tttaagttta cttataccta aatgttatgc gtcctttggt taaatcatag
      601 ggcgacatct ccactctaac cttatcgccg ggtagtattt tgatgtagtg catgcgcatt
      661 ttacccgata tatgcgcaat aataacatgg ccgttgtcaa gctccacccg aaacattgcg
      721 tttgacaatg cttcaactat ggttccatct ttttctattg ccggttgttt cgacatccgc
      781 tcgccttatt aacttttagt aattacctgt tcaacgtact ggaaagtgga gagggtttcg
      841 gcttttccat tcctcactac tacggcaagt tcgtagtgtg cagatggttt tccatcctgc
      901 gttcgaactg cccacccatc actatcctgg taaacatacc ttgttcccag gttaatcatt
      961 ggctcaatac atataaccaa acctgccgga agtttaggtc cgcgcccctt attgccataa
     1021 ttgggaacct cggggctttc gtgcatgtcg cgcccaaggc catgaccaac cagctcccga
     1081 accacggagt agccattact ctcggcatgg gtttgaatcg catgtgaaac atcgcctatg
     1141 cgggcaccct caaccgctgc ttctacaccg tgctccaggc actcgcgggt aaccctgagc
     1201 aaccgtttaa cctgtggagc cacatcacca acttcaaatg tataggccga atcgccatag
     1261 aatccatgca tgtaggttcc gcagtcaacc gaaacaatat ctccctcgtg cagcacccgg
     1321 ttagaaggaa ttccatgaac caccacatcg ttaacggata cacaaagagt tgccggaaac
     1381 ccgtcgtagc cgagaaagcc aggctttgcg ccattatcac g
//
LOCUS       DAOOQX010000582         1419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_122357_length_1419_cov_2.874633, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000582 DAOOQX010000000
VERSION     DAOOQX010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122357_length_1419_cov_2.874633"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLX53_08605"
     CDS             <1..307
                     /locus_tag="PLX53_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73180.1"
                     /translation="IPNDGNDASNNRAHPTDEDAECCALKHDYLIPDDGFEYKPAVTN
                     IHHGFNIAGGFITSCASIPLQIFLVNTRLYGGYTNNFHFSSYVSIPGLRSPPAKNLL"
     gene            530..>1419
                     /locus_tag="PLX53_08610"
     CDS             530..>1419
                     /locus_tag="PLX53_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73181.1"
                     /translation="MKRLFILIFSITIYGCSHTADSSAGAAHQHDESSHEAEAGDDAQ
                     TFTIYTSTCELFAEAKPFVVGTTTTVLSHFTNLADFKPLTSAAITIKLTVNGKEYSQT
                     IAIPEKNGIYHFTVTPDLSGQGQLVWIVERDGKSELFDAGLVRVFANEHEAEAFHAEH
                     IPADAIRFTKEQSWQVDFSTEWPCMEAIGEVIKTTARVEPAPTDEMVISAKTGGIVSF
                     YSSKPLVLGQHVERGDKLLEIVSSGLANEDLSVRYAEAKSNFERAKSNYERILELSKS
                     KVASEKELGIALNEYQNASST"
BASE COUNT          407 a          283 c          319 g          410 t
ORIGIN      
        1 aattccgaac gatggaaatg atgcaagcaa taaccgggct catcctaccg acgaggatgc
       61 tgaatgctgt gcgctaaagc atgactacct gattcccgac gatgggtttg aatataaacc
      121 tgcggttacc aatattcatc atggctttaa catagctggt ggctttatta cttcttgtgc
      181 ttcaattcct cttcaaatat ttttggtaaa cacaaggttg tatgggggtt atactaacaa
      241 ttttcatttt tctagctacg taagcattcc tggcctacgg tctcctccgg ctaaaaacct
      301 cttatagcct aatcttttcc aaccacctaa atggtggatc ttcctgtaat ttactcaagt
      361 ttaagtgact gtttgtgaat cagctgcgtt tcgacgttta gctgcttgca ggtaaatagt
      421 tcatggagga tgatttcaaa aaaatcttta agttttcctg cattcaacgt aaacgcttga
      481 aatgcaaaaa tatacgtaat tacagtgtaa cttaaaattt tatatacgga tgaagcggtt
      541 attcattctg atttttagca ttacaattta tggctgtagc cacactgccg atagcagtgc
      601 tggtgcagcc catcaacatg acgaaagtag tcatgaagct gaggccggtg atgatgctca
      661 aacctttacc atttatacat caacctgtga gctatttgcc gaagctaaac cttttgttgt
      721 tgggacaact actacagtgt tgtcccattt tactaatctt gccgatttta aacctcttac
      781 aagtgcggct attaccataa agctgacggt gaatggtaaa gagtactccc aaacaattgc
      841 catccctgaa aaaaatggta tttaccattt tacggttaca cccgatctgt ccggacaggg
      901 acagctggtg tggattgttg aaagggatgg taaaagcgag ctctttgacg caggcttggt
      961 tagggtgttc gctaacgaac atgaggcgga agcgtttcat gctgaacata tacctgccga
     1021 tgcgattaga tttactaagg aacaatcgtg gcaggttgac ttttcaaccg aatggccatg
     1081 catggaagcc ataggcgagg taattaaaac taccgcaagg gttgaacctg caccaaccga
     1141 cgaaatggtt atttccgcaa aaactggtgg tattgtatct ttctatagca gtaaacctct
     1201 ggtgctaggg cagcatgtcg agcggggtga taagttgctg gaaattgtaa gtagtggttt
     1261 ggccaatgag gatttgagcg ttaggtatgc cgaggcaaaa agcaacttcg agagggctaa
     1321 gtctaactac gaacgaattc tcgagctttc taaaagtaag gtggcttctg aaaaagagct
     1381 gggcattgca ctaaacgagt atcaaaacgc aagttcaac
//
LOCUS       DAOOQX010000583         1418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_122563_length_1418_cov_2.134263, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000583 DAOOQX010000000
VERSION     DAOOQX010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122563_length_1418_cov_2.134263"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PLX53_08615"
     CDS             <1..737
                     /locus_tag="PLX53_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73182.1"
                     /translation="SYWINHGIDYDYLYNGSYYYGRRPGESSFNTSSRRTASGFAPAA
                     SQAVTRQRGEVQSSSINPIQSTKNTQTRVTPTYENNRRSVEGTQRSGEQITAPRRETG
                     TNLSNPSSTRGSAVSSGTRSERPTRSIGISEPTGRAYNPGYDKPRTSSSGTQSIERPS
                     TGRGTSTSPSGRSSSSPTYNSPGRVSAPSNGSATRSGNSVQSKPEGSKSSGNSSGTYT
                     PRTESSSSGSKGNSNSGSSGAKTRGR"
     gene            848..>1418
                     /locus_tag="PLX53_08620"
     CDS             848..>1418
                     /locus_tag="PLX53_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73183.1"
                     /translation="MIRHISRFFLVLMASGTVTQLFSQTIEDGIRFSQTLNGGTARSI
                     SLGGAIGALGGDFTSLSINPAGLGVYKSGELTVTPSFKNQKVSSTYNGIKATDTRNRL
                     YFDNVGVVMSLKPNGSDDKGLVNLNMGVGYTRTNDFFSNSIATGDNPNNSIMDFFAVK
                     AKGYYYNDLLVDDNYNPFSESSAPWDVIMA"
BASE COUNT          423 a          364 c          304 g          327 t
ORIGIN      
        1 gaagctactg gataaaccat ggcattgact acgattacct ttataatggc agctactact
       61 atggccgccg ccccggtgaa tcatccttca acacttcttc ccgccgaaca gcatcaggat
      121 ttgcgccagc tgccagccag gctgttaccc gccagagagg cgaagtgcaa agcagctcca
      181 taaatcctat tcaatctacc aaaaacaccc aaacacgcgt aacacccacc tatgaaaaca
      241 acagaagaag tgtagaaggt actcaacgtt cgggcgaaca gattactgct cctcgcagag
      301 aaaccggcac taatctatca aacccttctt ctacaagagg ttcagctgta agttctggaa
      361 cgcggagcga aaggcctacg cgctccattg gaatatccga gccaaccggg agagcataca
      421 accctggcta cgataaaccc cgaaccagct catccggaac acagtctatc gagcgaccaa
      481 gcactggcag aggtacaagc acatcaccat cgggtagatc gtcttcctct cccacataca
      541 atagccccgg cagggtttca gcaccttcaa atggctcagc aacacgatca ggaaattcgg
      601 tgcagtcaaa acctgagggt tccaagtctt cgggaaattc cagcggaact tacactccaa
      661 gaactgaaag ttcgtcatca ggttcaaaag gaaatagcaa ctcgggatcc tctggagcca
      721 aaacacgtgg ccgataaaaa tctcttagct gtgtagcagg gaggaataac aaaattcctc
      781 cctttatcca gcaatttata cactgtggtg aaaaaacaat cattagcatc tacataaacc
      841 tattacaatg ataaggcata tttcgagatt ttttctggta ttaatggctt caggtactgt
      901 cactcaactt ttttcacaaa ccatagagga tggaattcga ttttcgcaaa cccttaatgg
      961 aggaacagcc cgcagcattt cgctgggagg agccattggt gccttggggg gcgatttcac
     1021 ctccctaagc atcaatccgg caggattggg tgtttacaag tcaggagagc taaccgttac
     1081 cccttcattt aaaaaccaaa aggtttcttc aacctataac ggcattaaag ctacggacac
     1141 aaggaatcgc ctgtacttcg ataacgtggg tgttgtaatg tcgcttaaac cgaacggaag
     1201 cgacgataag ggcttggtta acctaaacat gggagttggg tacacccgca caaacgactt
     1261 tttctccaac tccattgcca ccggcgataa tcccaacaac tccattatgg acttctttgc
     1321 tgttaaggct aaggggtact actataacga tttgctggtt gatgataatt acaatccatt
     1381 tagcgaaagc agtgctccct gggatgtgat tatggcat
//
LOCUS       DAOOQX010000584         1415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_123046_length_1415_cov_1.980882, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000584 DAOOQX010000000
VERSION     DAOOQX010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123046_length_1415_cov_1.980882"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..>1415
                     /locus_tag="PLX53_08625"
     CDS             336..>1415
                     /locus_tag="PLX53_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOY73184.1"
                     /translation="MQKLTFTFIVSLILTLGAAGQKISISLFNNLKLSTILITPTSGS
                     YRLILGDNEVELKVNQILYISRSGDSLMVRDANQNMGTWKRVTVVGQTGEDIIRVKPI
                     VPASPARLYDDNLSFYVDLDRIFAINLIDQEKYVAAVVEAEGGLNREPEFYKVQALIV
                     RTYTFAHLNKHLAEGFNLCDEVHCQSYLGRSEKNSDILDAVLDTKDMVIVDAERNPIT
                     AAFHANCGGQTANSEDVWVKPLPYLVSVSDRYCLGSNSSTWERSIPIGEWKKFLSQNG
                     VDTLKIKKNSDLDFQAKTRPKYYAVMGVEIPTTDIRKHFRLRSAWFSVSAGKSEVRLS
                     GRGYGHGVGLCQDGAINMAQKGWTYD"
BASE COUNT          390 a          289 c          339 g          397 t
ORIGIN      
        1 gccccgaaag ggaaaaaatg ctgaccagct atgctcagct tcaaaagcaa atggggggta
       61 gtggcgcttc catgcgggtt ggtgacaaga tagttgagct tatttcacgc tgattttatt
      121 gctcatcttg ttcattgata gcaaatctcc cccctacagc aattctttaa cagaatttag
      181 ttgttgccag cgcgctgttt ttgtaagcag gtgaatcaat attttacgtt ttacctatat
      241 tgcaccccaa tttgggaaat gaaatagtac atgctactta aagccttagg ttgcattaac
      301 agatacggat gtaagtcaaa tttcaacagt attttatgca aaagctaacc tttacgttta
      361 tagtatcgtt gatccttacg ttgggtgctg caggacaaaa gattagcatt agcctgttca
      421 acaaccttaa gctatcaacc attttgatta cgccaaccag cggcagctac cgcttaattc
      481 tgggtgataa tgaggttgaa ctaaaggtga accaaatact ttacattagt cgttctggag
      541 attccctcat ggtaagggat gctaaccaaa acatgggaac atggaagagg gttaccgtgg
      601 ttggccaaac cggtgaggac attattcgag taaaaccaat tgtacctgca agtccggctc
      661 gtttgtacga cgataacctg agtttctatg ttgacctcga ccgtattttt gccatcaacc
      721 taattgacca ggaaaagtat gtggcagctg ttgtagaagc tgaaggagga cttaatcggg
      781 aaccggagtt ttataaggtt caggcgttaa ttgttcgaac ttacaccttt gcccacctaa
      841 ataaacacct ggccgagggt tttaacctat gcgatgaagt tcactgccag tcatacctag
      901 gtcgaagcga gaagaatagt gatattctgg atgccgtact tgatactaag gatatggtaa
      961 ttgttgacgc cgaaaggaat ccaattactg ctgcattcca tgctaattgt ggaggacaaa
     1021 ctgccaactc cgaggacgta tgggtaaaac ccttgccata cctggtttct gtgtccgaca
     1081 ggtactgcct gggttcgaat agctcaacat gggagcgcag cattcccatc ggggagtgga
     1141 agaagttcct ttcacaaaat ggggtggaca cccttaaaat taaaaagaat tccgatttgg
     1201 attttcaggc caaaacccga ccaaaatact atgctgtcat gggggttgaa attcctacca
     1261 ctgacattcg taagcatttc aggttgcgtt cggcatggtt ttcggtttca gctggtaaga
     1321 gcgaggttcg cctttcgggt cgtggctacg ggcatggggt tggcctttgc caggatggtg
     1381 ctataaatat ggctcaaaag ggttggactt acgat
//
LOCUS       DAOOQX010000585         1414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_123153_length_1414_cov_2.463576, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000585 DAOOQX010000000
VERSION     DAOOQX010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123153_length_1414_cov_2.463576"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="PLX53_08630"
     CDS             complement(<1..147)
                     /locus_tag="PLX53_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOY73185.1"
                     /translation="MTKADIVNEISKTTGIEKVEAQKVVEAFMEIVKDSLAKEKNVYL
                     RGFGS"
     gene            283..1356
                     /gene="mutY"
                     /locus_tag="PLX53_08635"
     CDS             283..1356
                     /gene="mutY"
                     /locus_tag="PLX53_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOY73186.1"
                     /translation="MDFSALLLEWFSVHHRDLPWRNTSDPYHIWVSEVILQQTRVNQG
                     TEYYQRFIHIFPDIPALAEAPLDAVMKAWQGLGYYTRARNLHAGAKQVMSEFGGKLPT
                     SYSELLRIAGLGPYSAAAVASFAFNEAVPAVDGNVYRILARVFGVFTPVNSSQARREF
                     FNLAGELIDRNRPAEFNQAIIDLGALVCTPRRPSCSACPFSDYCYAYLNNLTGLLPLK
                     NRKNPPRSRYFTYLMINFNGTTFIRKREGNDIWHSLYEFPLIETPEQLDTTQLVETEA
                     WKELMGDSSAEIIHVSDPIKHQLSHITLHARFIIVQMRNPTYALRTGYRNISIGNLQE
                     LSVPRLIDSYIASEPAERYFTGK"
BASE COUNT          360 a          329 c          296 g          429 t
ORIGIN      
        1 gctgccgaac cctctgaggt aaacattttt ttcctttgcc agggagtcct tcactatttc
       61 catgaaggct tctacaacct tctgtgcttc taccttttct atcccggtag tcttggaaat
      121 ttcatttaca atatctgcct ttgtcatttc taaaagtatt taattatcaa tatgttacac
      181 atagtggatg atttcgaact gcaaatataa accttttatc tcgataattg aaacggcaag
      241 tattaatttt gacaagaatt tgttttgcta agtgctgatt gtatggattt tagtgcattg
      301 ctcctggaat ggtttagtgt acaccatcgt gatttaccct ggaggaatac gtccgatcct
      361 taccatattt gggtttcgga ggttatactt cagcaaacaa gggtaaatca gggaacagaa
      421 tactatcaaa ggtttattca tattttcccc gatataccag cattggccga agcgcccctt
      481 gatgctgtta tgaaggcatg gcaagggctg ggatactaca cccgtgcccg caacctgcat
      541 gcaggtgcca aacaggtaat gtccgaattt ggtgggaagc tgcccaccag ctactccgaa
      601 cttctgcgta ttgccggact cggaccatac tcagccgctg cagtggcttc gtttgccttc
      661 aacgaggctg tgcctgctgt ggatggaaat gtttacagaa ttctggctcg tgtgtttggt
      721 gtgtttactc cggttaactc ctcacaggcc cgaagggagt tttttaacct tgctggtgag
      781 cttattgaca ggaatcgccc tgctgaattt aaccaggcta ttattgacct gggtgcattg
      841 gtttgcacac cgcgcaggcc ttcgtgttca gcctgccctt tttccgacta ctgctatgcc
      901 tatttaaaca acctgactgg gttgctcccc cttaaaaaca ggaagaatcc accccgatca
      961 cgatacttca cctaccttat gattaacttt aacggaacaa cctttatccg caagcgggag
     1021 gggaacgata tatggcactc cctgtatgaa tttccgctca ttgaaactcc agaacaactt
     1081 gataccactc agctggttga aaccgaagcg tggaaagagc taatgggcga ttcatcagcc
     1141 gagattattc atgtgtccga cccaatcaag catcagctta gccatataac cctgcatgcc
     1201 cgttttatta tcgtgcaaat gagaaacccc acctatgcct tacgaaccgg atatcgaaac
     1261 attagcattg gaaacctaca agagttgtcg gtaccgcgtt taattgattc atacatagct
     1321 tctgaacctg ctgaacgcta ctttacggga aagtgaaatt tatttgcttt gtatattttt
     1381 taagtattta atactatcgt cttttgaggc atcg
//
LOCUS       DAOOQX010000586         1413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_123269_length_1413_cov_2.955081, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000586 DAOOQX010000000
VERSION     DAOOQX010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123269_length_1413_cov_2.955081"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLX53_08640"
     CDS             complement(<1..226)
                     /locus_tag="PLX53_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HOY73187.1"
                     /translation="MARMKPTLKENKPKKLLVIRLSAIGDVAMTVPVLWSVVKHNPDV
                     RVTFVSQSFARDIVSQIPGIQFYEADTKNRH"
     gene            complement(201..824)
                     /locus_tag="PLX53_08645"
     CDS             complement(201..824)
                     /locus_tag="PLX53_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HOY73188.1"
                     /translation="MVKNFSELCNNIFDECTKYYHTMDNVDANPNVPYKSNTIEHSLF
                     IKNWIDAVQWHLEDLIRDPNINPEKALLLKRRIDKSNQDRTDLVEQIDSYFLEYFKDV
                     RPQNGATINTESPAWAVDRLSILALKIYHMEQEVSRTNASADHVATCRQKLTILLEQR
                     KDLSMAIDQLLDDIANGRKYMKVYRQMKMYNDPSLNPILYGKNETNP"
     gene            complement(1091..>1413)
                     /locus_tag="PLX53_08650"
     CDS             complement(1091..>1413)
                     /locus_tag="PLX53_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOY73189.1"
                     /translation="LGRRLGEWSNSYGNIATERKQYVGKISNYFSDMGGAELNVEATE
                     LYLNDKVLIIGPTTGVIETLVSEIRVDNQQASFAPKGLPCSIPVPSKVRRSDKLYRII
                     YSKP"
BASE COUNT          394 a          286 c          282 g          451 t
ORIGIN      
        1 tatggcgatt cttggtgtcg gcttcataaa attgaattcc aggaatttgg ctaactatgt
       61 cgcgagcaaa gctctgcgat acaaaagtaa cgcgaacgtc agggttgtgc tttactaccg
      121 accagagaac aggaacggtc atggcaacat caccaatggc gctgaggcgg attaccaaaa
      181 gtttcttcgg tttgttctcc ttaagggttg gtttcattct tgccataaag aatggggttc
      241 agagagggat cgttatacat tttcatttgc ctgtatacct tcatatactt tctgccattg
      301 gcaatgtcgt ccaacaactg atctattgcc atggagagat cttttcgttg ctccagtaaa
      361 atagttaatt tttgtcggca agtagccaca tgatcagctg aagcattagt tctgctaacc
      421 tcctgttcca tgtggtaaat ttttagggct agaatggaga gcctgtcaac tgcccatgcc
      481 gggctttcgg tattgattgt agccccattt tgggggcgaa catccttaaa gtattctaaa
      541 aagtagctgt caatttgctc aaccaaatcg gttctatctt gatttgactt gtcgattctt
      601 cgttttagca gaagcgcttt ttcgggattg atgttcgggt cgcgaatcaa atcttccagg
      661 tgccactgaa ctgcatcaat ccaattctta atgaagaggg agtgctcgat ggtgttactt
      721 ttataaggaa cgtttgggtt agcatccaca ttatccatag tgtggtagta ttttgtgcat
      781 tcatcaaaaa tgttattgca caactcgcta aaattcttaa ccatagttaa tttatattaa
      841 gttacttaat gatatattta aataaattca atgattaaca attcaaaaag atcttttgtg
      901 gttattacaa tcaccgattt aagcaatgtg ccgaaatcct tgtaaaaatc tttaaaccat
      961 catctaaaat aaaaatttca acttgagcat ttataatttt gtttatcaat catgtattga
     1021 tgtttctgaa aatatcactt aaaacattct acccattcct aagttaacgc ttgggtggta
     1081 taagctgcaa tcaaggctta gaataaataa tcctgtaaag cttatcggag cgacgaacct
     1141 ttgatgggac aggaatagag cacggaagtc cctttggagc aaagctggcc tgctggttat
     1201 caacacgtat ttcggatacc agtgtttcaa taacgccggt agtaggccct attattaaaa
     1261 ccttatcatt aaggtatagc tccgttgcct caacgtttag ctcggcacct cccatgtcgg
     1321 aaaagtagtt gctaattttt cccacatact gcttgcgctc cgttgcgata tttccatagg
     1381 aattgctcca ctcccccaac ctgcgcccaa ggt
//
LOCUS       DAOOQX010000587         1413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_123292_length_1413_cov_2.502209, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000587 DAOOQX010000000
VERSION     DAOOQX010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123292_length_1413_cov_2.502209"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PLX53_08655"
     CDS             complement(<1..671)
                     /locus_tag="PLX53_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73190.1"
                     /translation="MERKSSKLEELAMSGSPIQLAIRRLKASWIAVASFCLIGIVVLI
                     SILGYLITPDPTPYANNQHLELSSCKPGAEVTMLKVRRQQDIQHRSWFGRMLYGDESD
                     YREIPINSFYFEDASIVIEGYNPIPESEPVVARFSLAEVAYRIEPPAKPIIRNDSVLF
                     VQADGGATAMTVSQLQERVEMENFVTHTYILGTDRFGRDLLSRMIIGARVSLSVGFIA
                     VAISLL"
     gene            complement(638..>1413)
                     /locus_tag="PLX53_08660"
     CDS             complement(638..>1413)
                     /locus_tag="PLX53_08660"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY73191.1"
                     /translation="FGVELARFNLVAITDADCVPAATWLNDISRRANGYALLQGCVLV
                     ERKPNLLSWFEALDYASLMAVSAGSFGLKRPVVAASANLVFRKDMINVRPETLRVDVA
                     SGDDMFLLHEVKKDHSNNMLFSTNLAGSVLTRFDGGFVGAIRRRRRWASKAPAYTDIE
                     TILVGLIVLVFNLWLLVLPMICIFNLEAWNLLVGSLLVASLANFCLLFIYLKQTNQLK
                     LILVFLPLQLIYPIYLCTVAIAGFAGGQNWKGRAVSWKN"
BASE COUNT          413 a          351 c          321 g          328 t
ORIGIN      
        1 agcagggata tggcaacggc aataaatcca actgacaggg aaactcttgc accaataatc
       61 atcctgctca gcaggtcgcg gccaaaccga tcggtgccca gaatataggt gtgggtaaca
      121 aaattctcca tctccaccct ttcctgcagc tggcttaccg tcatagctgt tgctcctcca
      181 tcagcttgaa caaaaaggac agaatcgtta cggattatcg gcttagcagg tggttcaatt
      241 ctatacgcca cctctgcaag agaaaatctg gctacaacag gttccgattc cggaatggga
      301 ttatacccct caatcacaat gctggcatct tcaaagtaaa agctattaat gggaatttcc
      361 ctataatcgg actcatcgcc atagagcatt ctcccaaacc atgagcggtg ctgaatatcc
      421 tgttgtcttc taactttaag catggtaacc tcagctcctg gtttgcagga tgacagctcc
      481 aaatgctggt tgttggcgta gggtgttgga tcgggagtaa tcaggtatcc taatatggaa
      541 atcagcacaa caatcccgat caggcaaaat gaggcaacag caatccagct ggctttcaac
      601 cgtcggatgg ctagctggat gggtgaacca ctcatggcta gttcttccag cttactgctc
      661 ttcctttcca attttgtcct ccggcaaaac cagctattgc gaccgtgcat aggtaaatgg
      721 ggtaaatcag ctgtaaaggt aaaaaaacaa gtatcagctt cagctggttt gtttgtttca
      781 ggtatatgaa gagtaggcaa aagttagcca gtgaggcaac caacaagctg ccaaccagta
      841 ggttccatgc ctctagatta aagatgcaaa tcatcggtag aactagcaac caaaggttaa
      901 aaaccaaaac tattagcccc acaagaatgg tttcgatatc ggtgtaggca ggggctttgg
      961 atgcccaacg gcgacgacgt cgaattgccc caacaaaacc tccgtcaaaa cgggttagta
     1021 cgcttccggc gagattagtg ctgaatagca tgttgttcga gtggtccttc ttaacctcgt
     1081 ggagcaggaa catatcatca cccgatgcaa catcaacacg gagcgtttca ggacgcacgt
     1141 taatcatatc cttacgaaaa acgaggttag cggaagctgc aactacaggg cgctttagcc
     1201 cgaacgaccc cgccgaaaca gccattagcg aggcgtaatc caacgcctca aaccaggaga
     1261 ggaggtttgg tttcctttca actaaaacgc aaccctgtag caaggcatac ccattggccc
     1321 ttcgggaaat atcgttaagc catgtagcag caggaacaca atcggcatca gtgatagcta
     1381 ccaggttaaa cctagccaat tcaaccccaa ata
//
LOCUS       DAOOQX010000588         1412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_123426_length_1412_cov_2.418570, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000588 DAOOQX010000000
VERSION     DAOOQX010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123426_length_1412_cov_2.418570"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /gene="tsaD"
                     /locus_tag="PLX53_08665"
     CDS             complement(<1..696)
                     /gene="tsaD"
                     /locus_tag="PLX53_08665"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOY73192.1"
                     /translation="MSITILGIESSCDDTSAAVIRDGFLLSNLISSQEVHREYGGVVP
                     ELASRAHQANIIPVVDGALKKAGVGIGEINAVAFTQGPGLMGSLLVGSSFAKGVALSR
                     QIPLVAVNHLQGHVLAHFISDDKAQDHPSFPFLCLLVSGGHTQLIIVRKPLEMEIVGQ
                     TIDDAAGEAFDKCAKVMGLPYPGGPVVDKLAAGGNIHAFRFARPVIKGLDFSFSGLKT
                     SFLYTLRDRVAGDP"
     gene            789..>1412
                     /locus_tag="PLX53_08670"
     CDS             789..>1412
                     /locus_tag="PLX53_08670"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOY73193.1"
                     /translation="MRIKFRFTIPINIGKGIKKTAKILLRVVIVLLAVPSLIWLTLQN
                     SRIQTYLVDQVTASLKERLGIEVSVGRVDFRPFASLLLRDVFIADLKGDTLLHSGRIA
                     VTLLGLKLAPSNIELGRANFDQTTFNFVTDSSGVMNLSGLIRKIKETFPSKKDSSDFR
                     LSANSISISNSRFILKKTAPKARNSDFGMNFQDMNFSNFNLKADNLYI"
BASE COUNT          413 a          353 c          297 g          349 t
ORIGIN      
        1 agggtcgcca gcaaccctat cgcgcagggt atataaaaac gacgttttaa gtccactaaa
       61 gctgaagtca agtcctttaa ttacgggacg ggcaaatcta aaagcatgaa tgttcccccc
      121 ggcagccagc ttatcaacca caggccctcc cgggtagggt aaccccatca ccttggcaca
      181 tttgtcgaaa gcttcgcctg cagcatcatc aatagtttgc ccaacaatct ccatctcaag
      241 gggcttacga acaataataa gctgagtatg cccacccgaa accagcaggc ataggaatgg
      301 gaacgatggg tgatcctgag ctttgtcgtc gctgatgaag tgggcaagca catgcccctg
      361 caaatggtta actgcaacaa ggggaatttg acgcgacaag gctactccct tggcaaacga
      421 actcccaacc aggagcgaac ccattaagcc gggaccctgt gtaaaggcaa ctgcattgat
      481 ttcgccaata cccacaccgg ctttttttag cgctccgtca actactggaa ttatattcgc
      541 ctggtgagct ctcgaggcaa gttccggaac aacgcctcca tactccctat gcacctcctg
      601 gctgcttatt aggttggaga gcaagaatcc atcgcgaatc accgctgcag aggtgtcatc
      661 gcatgacgat tcaatcccca gaatagttat gctcatcgtt aatttttatt aaaccatcaa
      721 attgattgaa acattcggaa tggacaattc caaaaaacat ttctaattgc ttactttcgg
      781 aacgaagttt gagaattaaa tttaggttta ctatacccat aaatataggc aaaggtatca
      841 aaaaaacagc aaaaatattg ttaagggtag tcattgtcct gctggccgta ccctcattaa
      901 tctggttaac ccttcagaac agccggatcc aaacctatct ggttgaccag gtaaccgcct
      961 cccttaaaga gcgcttagga attgaggtat cggtaggcag ggttgatttt cgcccatttg
     1021 ccagcttgct tctaagggat gtttttattg ccgacctaaa gggcgacaca ctcctacatt
     1081 ctggcaggat agccgttacc ctgcttggcc ttaaactggc acccagcaac atcgagctgg
     1141 gaagggcaaa cttcgatcag accactttca actttgttac cgattcgtcg ggcgttatga
     1201 accttagcgg gttaattaga aagataaaag aaacctttcc ctcaaaaaaa gattcatcgg
     1261 atttcaggct cagcgctaac agtatttcta ttagcaactc caggtttata ctgaaaaaaa
     1321 cagctccaaa agccagaaat tcggatttcg gcatgaactt tcaggatatg aacttcagca
     1381 acttcaacct gaaggctgac aacctataca tt
//
LOCUS       DAOOQX010000589         1409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_123830_length_1409_cov_3.536189, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000589 DAOOQX010000000
VERSION     DAOOQX010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123830_length_1409_cov_3.536189"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(329..1243)
                     /locus_tag="PLX53_08675"
     CDS             complement(329..1243)
                     /locus_tag="PLX53_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOY73194.1"
                     /translation="MMLNKMIAAMLPYLPKKLIWVFSKRYIAGETVNDAIRVARDLNH
                     KGVKVTLDILGEFIKTLEEAEKNKQEYLSLIDIIQKEKIDGNYSVKPTMFGLLIDKEV
                     CYQHLREIVAKAASYNSFIRIDMEDSSCTDLEIELYRRLKSEYPKNVGLVVQAYLKRT
                     LDDLKKLLDLNSKEIPMNYRLCKGIYVEPAEIAFKKYEEVNQHYLEDLEFMFQNGIYA
                     CIATHDKPLVEGAYKLIEKYKVPSNMLEFQMLYGVTPELRDSIVGKGYTMRVYVPFGK
                     QWFGYSTRRLKENPKMTSHIIKALFVRG"
BASE COUNT          367 a          287 c          295 g          460 t
ORIGIN      
        1 ctgctggccg atacgaaatt tctggttgag tagctttcgc aaccttccag aaaggagcaa
       61 aactccttgg ccaccgttaa aaactccaac gtattacggc cgtaaacagt tgcttttata
      121 tccatctctc accattatga gctacaaaga tagctattgt ggtgaggtct tttgtaggat
      181 agcttacaaa taaacgaatg acagcatttt taacagccta acgatggctg aaactgccca
      241 atttgttttg gtacagtagc acataaaccc ggtagggtgg tgaaaattgt ggagagtttt
      301 agtcgggaat ttctgaatgc aggtggtgct accctctaac aaacagggct ttaataatat
      361 ggctggtcat ttttgggttt tctttcagcc tacgggtgga gtagccaaac cattgcttcc
      421 cgaatggaac gtaaaccctc atggtatatc ccttacccac aattgagtct cgaagttcag
      481 gagttacacc gtaaagcatc tggaattcga gcatattgct ggggactttg tacttttcta
      541 tcagcttata tgcaccttca actaggggtt tgtcatgggt tgcgatacag gcgtatattc
      601 cgttttggaa catgaactcc aaatcttcca ggtaatgctg gtttacttcc tcgtattttt
      661 tgaatgctat ttctgcaggt tctacgtata tgcccttgca aaggcggtag ttcatgggaa
      721 tttcctttga attcaggtca agcaactttt tcaaatcatc cagggttctc tttaggtagg
      781 cttgtaccac caaaccaaca tttttgggat attctgactt gagcctccgg tatagctcta
      841 tttccaggtc ggtacatgat gagtcttcca tatctattct gatgaaactg ttgtaggaag
      901 cagccttcgc aacaatttcg cgtaaatgtt gatagcaaac ttccttatca atcagtagtc
      961 caaacatggt tggctttacg gagtagttac cgtcaatttt ttctttttgg atgatgtcta
     1021 tcagcgaaag gtactcctgc ttgttctttt ccgcctcttc aagagttttg ataaattccc
     1081 ccagtatgtc gagggtaact tttacgccct tgtggttgag atctcgggca acccttatgg
     1141 catcattaac ggtttcaccg gcgatgtagc gcttggaaaa aacccaaatc agctttttag
     1201 gaaggtaggg cagcatggct gcaatcattt tattcagcat cattttttaa tatttatgtt
     1261 gaaaacgaga atatggttaa gctatgaagt ccaacttgta catataaagt tcaaaaggtt
     1321 taaaaagttc aaagttttga tgtttaaaag ttgatgatga acatcttgtt tttggatttc
     1381 tttacccttc ccttgtcttc agccccagc
//
LOCUS       DAOOQX010000590         1408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_124063_length_1408_cov_2.209904, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000590 DAOOQX010000000
VERSION     DAOOQX010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124063_length_1408_cov_2.209904"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /gene="ricT"
                     /locus_tag="PLX53_08680"
     CDS             <1..490
                     /gene="ricT"
                     /locus_tag="PLX53_08680"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOY73195.1"
                     /translation="HFASVSTNSAKVQELSLNPQKLAGQCGKLKCCLNYELDSYMDAR
                     KDFPDTSIALETADATYYHFKTDIFRRSMWYGSEPSSSVNLIEVSVDRVNEIIALNRK
                     GIKADKLLENTTSDQNNSRPNGFTDGLLDEGSLTRFDKKGGKTAKRKKSRNRNRTRPS
                     NE"
     gene            483..962
                     /locus_tag="PLX53_08685"
     CDS             483..962
                     /locus_tag="PLX53_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOY73196.1"
                     /translation="MSKSLPIAFLAATLTLACCNRDAVFDSYVAIPRETWSADSLASF
                     NIAIPDTSQSYDIMFNLRNSTDYPFQNLFLFISIYSPNGGFVRDTFECFLADDNGRWT
                     GRGSGRIHDNRFIYREGVKFYQPGSYRFDLQQAMRVEDLRGVVDVGIRIEQSQQNFK"
     gene            1017..>1408
                     /gene="trmB"
                     /locus_tag="PLX53_08690"
     CDS             1017..>1408
                     /gene="trmB"
                     /locus_tag="PLX53_08690"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOY73197.1"
                     /translation="MGKNKLKRFAENETFSNLFQPPLEEVLNDKFELKGKWGNSYFKN
                     INPLVLELGCGKGEYTVSLAGMNPQKNFIGIDIKGARIWRGAKTALENKLNNVAFVRT
                     RIEHICSIFAQNEVDELWITFPDPQERE"
BASE COUNT          410 a          345 c          318 g          335 t
ORIGIN      
        1 acactttgcc tcggttagca ccaattccgc taaggttcag gagctgtcat taaatcctca
       61 aaaacttgct ggtcagtgcg gaaaacttaa atgctgcctt aactacgagc tcgacagcta
      121 catggatgcc cgcaaggact tccccgatac cagtattgct cttgaaaccg ccgatgccac
      181 ttactaccac ttcaaaaccg atatcttcag acggagtatg tggtacggca gcgagccttc
      241 ttcgtcggtt aaccttatcg aagtctctgt tgatcgcgtt aacgaaatta tcgccctaaa
      301 cagaaaggga attaaagccg acaagttgtt ggaaaatact acatccgacc agaataactc
      361 ccggccaaac ggcttcaccg atggtttact cgacgagggt agcctgaccc gatttgataa
      421 aaagggggga aagaccgcaa aaaggaaaaa aagcagaaat cgcaaccgaa ccaggccaag
      481 caatgagtaa aagcctacca attgcttttt tggcagctac gcttacattg gcttgctgca
      541 acagggatgc cgtatttgat tcatacgtag ctattccccg tgaaacttgg agcgccgata
      601 gcttggctag cttcaacatt gctattcccg acacttccca atcctacgac atcatgttta
      661 atctacgcaa ctccaccgac tacccgtttc aaaacctttt tcttttcatc agcatttact
      721 cccccaacgg tggctttgtc cgtgatacct tcgaatgctt cctggccgac gataatggtc
      781 gctggacggg taggggtagc gggagaattc acgacaaccg gttcatttac cgcgagggtg
      841 taaagtttta tcaacctggc agctacagat ttgaccttca gcaggccatg cgagttgaag
      901 acctcagggg agttgttgat gttggaataa gaattgaaca atcccaacaa aactttaaat
      961 aactagggca caagctactc agcccatatc acgtaaaaat cataactctc tgcaaggtgg
     1021 gtaagaataa gcttaagcga tttgccgaga acgaaacatt ctccaacctc tttcagcccc
     1081 cccttgagga ggtgcttaac gacaaattcg agctaaaagg gaaatggggg aacagctact
     1141 ttaaaaatat caaccctttg gttctggagc tgggatgcgg caaaggggag tacaccgtta
     1201 gcctggccgg tatgaaccca cagaaaaatt ttatcggtat cgacataaaa ggggccagaa
     1261 tatggcgcgg cgcaaaaacc gcacttgaaa acaagctgaa caacgtggcg tttgttcgaa
     1321 cccgcattga gcatatatgc tcaatttttg ctcaaaatga ggtggatgag ctgtggatta
     1381 cctttcccga ccctcaggaa cgagagat
//
LOCUS       DAOOQX010000591         1406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_124319_length_1406_cov_2.613620, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000591 DAOOQX010000000
VERSION     DAOOQX010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124319_length_1406_cov_2.613620"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLX53_08695"
     CDS             <1..546
                     /locus_tag="PLX53_08695"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOY73198.1"
                     /translation="ELSWRAYIQSRSLDEVTELAINKERMIECVPAIQPISVKNLVRI
                     SDFFGYRRDPITRAPTMHEGLDFAGPIGTPIYSTGDGIVIEAGYSFNGYGNQVMIDHG
                     FGYKTRYAHLSKVNVKVGERVKRAEVIGKLGNTGKSTGPHLHYEVIVRGKPVNPINYF
                     NDMTEQDYEAMLKNYSSQFLD"
     gene            564..>1406
                     /locus_tag="PLX53_08700"
     CDS             564..>1406
                     /locus_tag="PLX53_08700"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOY73199.1"
                     /translation="MAKYKYKFHPEQLTFVRVRATLKEKLFVALKYMVATAIVGIISY
                     LVFPFFIDTPEVRKLKRENQELMLNFELIDKRLDQLRAVIENLQQRDDNIYRIIFEAE
                     PIHPSVREAGMGGVDRYSYLEGLSNNKIIVETARKIDLLSKKAYIQSRSFDEISTLAK
                     RKEEMVRCIPAIMPINNKDLTRMASSFGMRIHPFYKVLKMHTGMDFTSPLGTEVYSTG
                     DGVVTNVEYAQRGYGYNIIVDHGFGYKTRYAHLGKIIVKPGQKVRRGMVIGLVGNTGT
                     SVAPH"
BASE COUNT          386 a          314 c          337 g          369 t
ORIGIN      
        1 gagctgtcat ggcgggccta catccagtcc cgttcactcg acgaggttac cgagttggcc
       61 atcaataaag agcgaatgat tgagtgcgtt cctgccattc agcccatttc tgttaaaaat
      121 ctggttagaa tatccgactt ttttggctat aggagagacc caattacccg tgctccaact
      181 atgcacgagg gtttggactt tgctgggcct attggaactc ccatttactc aactggcgat
      241 ggtattgtta ttgaggccgg ctactccttt aatggctacg gtaaccaggt tatgatcgat
      301 catgggtttg gctataaaac ccgctacgca caccttagca aggtaaacgt taaggtgggt
      361 gaaagggtta aacgggcgga ggtgataggc aagcttggca ataccgggaa gagcaccggt
      421 cctcatttac actacgaggt gattgttcgg ggtaaacccg ttaacccgat aaactacttt
      481 aacgatatga ccgagcagga ttacgaggcc atgctcaaga attattctag ccaattcctt
      541 gattaactat ctacattgga aaaatggcaa agtacaagta taaattccat cccgagcagc
      601 taacattcgt tagggttaga gctaccctta aagaaaagtt gtttgttgcc ttgaagtaca
      661 tggtggcaac agctattgtt ggtataatat cctacctggt tttccctttc tttatcgata
      721 cccccgaggt tcgaaagtta aagcgggaga accaggagct tatgcttaac tttgaactta
      781 tcgacaagcg gttggatcag ctcagagcgg ttattgaaaa cctacagcag cgcgacgata
      841 acatttaccg catcattttc gaggccgaac ctattcatcc ttctgtaaga gaagcgggga
      901 tgggcggtgt tgacaggtac tcctacctcg aaggcctttc aaacaacaag attattgttg
      961 aaactgcccg gaaaattgac cttttaagca aaaaggctta cattcaaagc cgttcgttcg
     1021 atgagatttc cactctggct aaaagaaagg aggaaatggt acgctgcatt cctgccatta
     1081 tgcctataaa caataaagac ctcacccgga tggcctcatc gtttggcatg agaattcatc
     1141 cattctacaa ggtgttgaaa atgcacacgg gcatggattt tacatccccg ttaggcaccg
     1201 aggtttactc aacaggcgat ggggttgtta ccaatgtgga gtatgcccaa cggggctatg
     1261 gctacaacat tattgttgac cacggatttg gctacaaaac ccgctacgcc catttgggga
     1321 aaattattgt taaacccggc caaaaagtta ggcggggcat ggtcattggc ctggtaggaa
     1381 acacgggaac ttctgttgcg ccccat
//
LOCUS       DAOOQX010000592         1405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_124504_length_1405_cov_2.179259, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000592 DAOOQX010000000
VERSION     DAOOQX010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124504_length_1405_cov_2.179259"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLX53_08705"
     CDS             <1..373
                     /locus_tag="PLX53_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOY73200.1"
                     /translation="LYSPDCWGLTASDDNNGYGAHEPLNDNGTITPTAALSSMPYTPE
                     ESMRVARFLYYTLGDKLWGDYGFYDAFNPTAGWWGKSYLAIDQGPIIIMIENYRTGLC
                     WNLFMSAPEVDGALSKLGFSH"
     gene            389..>1405
                     /locus_tag="PLX53_08710"
     CDS             389..>1405
                     /locus_tag="PLX53_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOY73201.1"
                     /translation="MRIRKLSEITLAAIMSLTILSCSKTTMPYSDVVDQKVDSILMLM
                     TLEEKIGQLNLVTSDMDVTGPFIRKGYEEDIAKGRVGAIFNAYGAGYTRKLQELAVNN
                     TRLRIPLLFGYDVIHGHKTIFPVPIGEASSWDLEAIERSARVAAEEASAQGLHWTFAP
                     MCDIARDPRWGRVVEGSGEDPLLGSLIAQARVRGFQGTDLSQTNTILACVKHFAAYGA
                     AQAGRDYHSVDMSERQLREVYLPPYKAAIDAGALSVMSSFNDLNGIPATASKYLMTDI
                     LRNEWGFQGFVVTDYTSIMELLHHGVAADTLDAARLALNAGIDMDMQSGFYNSALLKL
                     SKINI"
BASE COUNT          341 a          347 c          357 g          360 t
ORIGIN      
        1 gctctacagc cctgattgct ggggcttaac cgcctcggac gacaacaacg gctatggcgc
       61 tcacgaacca ttgaatgata acggaaccat cacccctacc gctgcactat cgtccatgcc
      121 ctatactccc gaggaatcca tgagggtggc tagatttcta tactacactt taggcgacaa
      181 gctatgggga gactacggtt tttacgatgc ttttaacccc actgccgggt ggtggggaaa
      241 gtcgtacctg gctatagatc agggaccgat catcatcatg attgaaaatt accgaaccgg
      301 gctgtgctgg aacctgttta tgtctgcccc ggaggtggat ggtgcactca gcaagcttgg
      361 tttttcacac taaaattgtt taaagatcat gcgtattagg aagctatctg aaattacact
      421 tgctgccatt atgagcttaa ccatactttc atgcagtaag acaaccatgc catactccga
      481 tgtggttgac cagaaggttg actctattct gatgctgatg actcttgagg aaaaaatcgg
      541 acagctaaac ctggttacca gcgatatgga tgttactggt ccattcattc gtaaggggta
      601 tgaggaggat attgcaaagg gtagagttgg ggccatattt aatgcctatg gcgcaggcta
      661 cacccgtaaa cttcaggagc tggctgttaa taataccagg ctccgcattc cgttactctt
      721 tggctacgat gtgattcatg gccacaaaac catattccca gttcccatag gcgaggcctc
      781 cagctgggat ttagaggcca ttgagcgttc ggctcgggta gctgccgagg aggcttcggc
      841 tcaaggccta cactggactt ttgcccccat gtgcgacatt gcccgcgacc cacgatgggg
      901 gagggttgtt gagggttcgg gcgaggatcc cctgcttgga tcactcatag ctcaggcaag
      961 ggttagaggt tttcagggaa ccgacttaag tcaaaccaac actatacttg cttgcgtgaa
     1021 gcattttgct gcttatggtg cagctcaggc tggccgcgac taccatagcg ttgatatgag
     1081 cgaacgacag ctgcgtgaag tttacctacc gccatataag gctgctattg atgccggcgc
     1141 actttcggtt atgtcctcat tcaacgacct taatggcatc cccgccacag ccagtaaata
     1201 ccttatgact gatatcctcc gaaatgagtg gggttttcag ggatttgtgg taaccgacta
     1261 cacctcaatt atggagctgc tccaccatgg agttgcggcc gataccctcg atgcagcccg
     1321 cctggctctc aatgccggaa ttgatatgga tatgcagtcg ggattctaca acagcgcctt
     1381 gttgaagctt tcgaaaataa atatc
//
LOCUS       DAOOQX010000593         1396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_125771_length_1396_cov_3.148397, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000593 DAOOQX010000000
VERSION     DAOOQX010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125771_length_1396_cov_3.148397"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLX53_08715"
     CDS             complement(<1..322)
                     /locus_tag="PLX53_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73202.1"
                     /translation="MNNFNVNPMNNIDSINHVTGKSIYLDDITTLKGTLHLEIFGSAV
                     AHAKILSIDLTYAARVEGVVGIFTAKDIPGENQIGGIVEDEVLLAETEVHYQGQPIAI
                     VAATT"
     gene            complement(344..>1396)
                     /locus_tag="PLX53_08720"
     CDS             complement(344..>1396)
                     /locus_tag="PLX53_08720"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOY73203.1"
                     /translation="TSYCLSHERPSIEGAINAVSGNICRCTGYKSIERAAADLNQIIR
                     DKNLVNPVGWMVEHGYLPAYFLDVPRLLGGIHPLDYTAPENSTYIGGATDLMVQKPNH
                     ILNDPILPVGALRGLKGIELDGSRCVIGAATTFNELTHSDLLCSIIPTLRNIMPFVAS
                     DQVRNSATIAGNIVNASPIADLSIILLSLNAEISTSLNGNMRIQPLREFYTGYKKLNL
                     YEGERVESISFEIPSPKSIFNFEKISKRKYLDIASVNSSILACTEGNVITSCHVSAGG
                     VAPIPTYLFNTSMFMVGKTINQETFEKANTIMQAEISPISDIRGSADYKRLLLRQLLF
                     SHLSKLFPELLKVTWL"
BASE COUNT          361 a          309 c          326 g          400 t
ORIGIN      
        1 ctgttgtggc tgcaacaatg gcaataggct gtccctgata atgcacctcg gtttcggcca
       61 gcaagacttc gtcctcaact attccgccaa tctggttctc gccgggtata tcttttgccg
      121 tgaatattcc tacaacaccc tccacccttg cagcataagt aaggtcaatg cttaaaattt
      181 tcgcatgggc cactgctgaa ccaaatattt ctaaatgaag tgtccccttc agggtggtta
      241 tatcatccag gtaaattgat ttgccggtta catgattaat ggagtctatg ttgttcatcg
      301 ggttcacgtt gaagttattc atccaatgtt cattcaaaat atgctaaagc catgtcacct
      361 ttaacagttc agggaaaagc ttcgagagat gtgaaaaaag gagctgcctc agaagcaacc
      421 ttttgtaatc agcactccct cttatgtcgg aaattggcga aatttcggcc tgcatgatgg
      481 tgttggcctt ctcaaaagtc tcctggttaa tggtttttcc taccatgaac atggaggtat
      541 taaaaagata ggtgggtatt ggagccaccc ctcctgctga aacatgacag ctggtgatga
      601 cgttcccctc agtgcaggca agaatggatg agttgacgct ggcaatatca agatactttc
      661 gcttgctgat tttctcaaag ttgaatatag attttggtga tggaatctca aagctgatgc
      721 tctcaacacg ctcgccttca tacaaattca actttttgta gccggtgtag aattccctaa
      781 ggggttgaat cctcatattt ccatttaacg aggtggaaat ttctgcattt agcgaaagca
      841 ggatgatgct gaggtcggca ataggggagg cgtttacaat gttaccagca atggttgcac
      901 tgtttcttac ctgatcggaa gctacaaagg gcatgatgtt tctaagggtt ggaatgatgc
      961 tgcataaaag gtcagagtgt gtaagttcgt taaatgttgt ggcagcgcca atcacgcacc
     1021 tgcttccatc caattcgata cccttaagcc ccctaagagc gccaactggc aatataggat
     1081 catttagaat atgattgggc ttttgcacca tcaaatcggt tgcaccgcca atgtaggtag
     1141 aattttcggg agcggtataa tcaagcggat gaattccgcc aagcagcctc gggacatcaa
     1201 ggaagtatgc tggcaggtaa ccatgctcta ccatccagcc caccgggttg accaggtttt
     1261 tgtcccgaat aatttgattt aaatcggctg ctgccctctc tattgatttg tagccggtgc
     1321 atcggcaaat gttaccgctt acagcgttta tagcgccttc gatgctcgga cgctcatgac
     1381 ttaggcagta ggaggt
//
LOCUS       DAOOQX010000594         1394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_126082_length_1394_cov_3.825243, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000594 DAOOQX010000000
VERSION     DAOOQX010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126082_length_1394_cov_3.825243"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1370
                     /locus_tag="PLX53_08725"
     CDS             15..1370
                     /locus_tag="PLX53_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOY73204.1"
                     /translation="MKSSYCLTEPSSGSDALSAKSTAILNVEGTHYILNGQKMWITNA
                     GFADTQVVFAKIENDRVLSAFIVESKWPGVVVGPDEHKMGIKGSSTAQIYYNDVKVPV
                     ENLLGNRGEGFRIALSILHMGRMKLGANVIGAAKETITQSVRYANERKQFNTQIANFG
                     SIKHKLAEQVIRTFAHESAIYRVSQNIDELITRYKAEGCDYGKAAIDAISHYAVEDAI
                     LKVNGSEMLDFVVDEGVQIHGGMGYSAEMNVERGYRDSRINRIFEGTNEINRLLVVDT
                     AIKRSLKGDFNLMGPAEELYTNLDGIKVEMGAAGYFEQKKQLIRNFKKIALLGIFGAN
                     KTFAKQFVSEQEVQNNLSNIIMDIYVAESLALRVEKLESRGHASMDIYRSILDVFIFD
                     AASRIRKNAIDAISSFAAEPELGKLIAATERLSAVNPINVKEARRKIADKLIADNVYK
                     F"
BASE COUNT          422 a          294 c          344 g          334 t
ORIGIN      
        1 tgatcacagg agagttgaaa tctagttatt gtcttacaga accttcaagt ggatcagatg
       61 ctttatccgc aaaatccacc gccatattaa atgtagaagg aacacattat atcttaaatg
      121 gccagaaaat gtggattacc aacgccggat ttgcagatac ccaggtggta ttcgcaaaaa
      181 ttgaaaacga cagggtgcta agcgccttca ttgtggaaag taagtggccg ggcgttgtgg
      241 ttggccccga tgagcacaag atgggaataa aaggatcctc taccgctcaa atctactata
      301 acgatgtgaa agttccggtc gaaaacctgt tgggaaaccg tggtgagggg ttccgaattg
      361 cccttagcat tcttcacatg ggacgaatga agctgggggc taacgttatt ggggcagcca
      421 aggaaaccat aacccaatcg gttcgttacg ctaatgagcg caagcaattc aacacgcaaa
      481 tagccaactt tggctccatt aagcataagc tggccgagca ggttatcaga acatttgccc
      541 acgaatcggc aatctaccgg gttagtcaga acatcgacga gctgattaca cgctacaaag
      601 ccgaaggttg cgactacggc aaggcagcta ttgatgccat aagccactac gctgttgagg
      661 atgccattct gaaggtaaac ggttctgaaa tgctcgactt tgtagttgat gagggtgttc
      721 aaatccatgg cggaatgggc tactctgccg aaatgaatgt ggaaagaggc taccgcgact
      781 cccgtatcaa ccggattttt gagggaacca acgaaattaa ccgcctgctg gtagttgaca
      841 cagcaataaa gcgctcgctg aaaggcgact tcaaccttat gggaccagcc gaggaacttt
      901 acaccaacct ggatggaatt aaggtagaaa tgggtgctgc tggttacttt gagcagaaaa
      961 aacagctcat caggaacttc aaaaagattg ccctgctggg gatttttggt gccaacaaaa
     1021 cttttgcaaa gcagtttgta agcgagcagg aggttcaaaa taacctcagc aacatcatca
     1081 tggatattta cgttgccgaa tcgcttgccc taagggtcga aaaactagag agcagaggtc
     1141 atgcttcaat ggatatatac aggagtattc tggacgtttt tatttttgat gctgcctcca
     1201 gaatccgtaa aaatgccatt gatgccatat catcctttgc tgctgagcca gaactaggta
     1261 agctgattgc tgctaccgaa cgcctaagcg cagtaaaccc cattaatgtt aaggaagccc
     1321 gtagaaaaat tgccgataag ctgattgccg ataatgtgta taagttctaa ttgaggggag
     1381 taagaatgct aaaa
//
LOCUS       DAOOQX010000595         1389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_126809_length_1389_cov_3.931784, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000595 DAOOQX010000000
VERSION     DAOOQX010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126809_length_1389_cov_3.931784"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..>1389
                     /locus_tag="PLX53_08730"
     CDS             255..>1389
                     /locus_tag="PLX53_08730"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HOY73205.1"
                     /translation="MFRFYRCLKFAAIAVLSFFCTTLHAQTDTEFWFVVPEITISHDP
                     PGGQPASFRISTGLLPATVTISMPANQYDPDTNPAGFPDIVFNMPDSSFHIEDLTCWI
                     MSPCSPPTGASTDINKLENKPINASGINNFGILITATNPITAYWEVSRTNNKDIWALK
                     GHNALGTDFYTPFQTHKDNQSINPVQPYSAIDVVATEDFTTVTFDLPAGIAASYGQPM
                     TNVPAGGILNVNLMRGETFSLFPINKSRAAADRLRGTHVTSNNPIAITLKDDSFLHTS
                     GGCYDVAGDQLVPTSIAGKEYAVIRTFLNNHDHIYILGTVDGTIVTVYNTGGDIVAPS
                     PTTINAGQQLYYQLPAGQTYYRIVADKPVYVWHVGGFGCEQGGA"
BASE COUNT          359 a          370 c          318 g          342 t
ORIGIN      
        1 ccccgttggg gctgaagaca aggataaagg ttaaaggata aaggataaaa aattgcctgg
       61 ctaacgacga aacttgtccg gctcctggcg gattcaccct tcgacaggtg ttacagtgag
      121 gcctttcact ctttatcatg cccaatttaa cttttgatga accaacaatt tatggtggta
      181 aacgtataaa cattggatat aaattttcgt attttataaa ctaatgtatt tttgttctgt
      241 caattccgga ttgcatgttt agattttatc gttgcctgaa gttcgcagct attgcggttt
      301 tgagtttttt ttgcactacc ctccatgccc aaaccgacac ggagttttgg tttgttgtgc
      361 ccgaaatcac tataagccat gatcctcccg gaggccagcc tgccagcttt agaatatcca
      421 cggggctgct gccagcaacc gttaccataa gtatgccggc caaccagtat gatccggata
      481 ccaaccctgc tggtttccct gatatcgttt tcaacatgcc cgacagctcg ttccatattg
      541 aggacctcac ctgctggata atgtccccct gttccccgcc caccggagca tcaactgata
      601 taaacaagct ggagaataag ccgataaacg cttcgggcat aaacaatttt ggaattctca
      661 taaccgccac caaccccatt acagcctact gggaagtttc cagaaccaat aacaaggaca
      721 tctgggcgct gaaggggcat aacgccctgg gcaccgactt ctacacgcct tttcaaactc
      781 acaaggataa tcaaagtata aatccagtgc agccctactc ggccattgac gtggtggcca
      841 ccgaggattt caccacggtt acctttgatc tcccggccgg tattgcagcc tcatacggcc
      901 aaccaatgac caacgtcccg gctggtggta tcttaaacgt taacctgatg cgtggcgaaa
      961 ccttttcgct attccccata aacaagagca gggcggcagc cgataggcta aggggcaccc
     1021 acgttaccag caataacccc attgccatca ccctgaagga tgattccttc ctgcacacat
     1081 caggtggctg ctacgacgtg gccggcgacc agctggttcc caccagcatt gcaggaaagg
     1141 agtacgcggt gatcagaaca tttttgaata atcatgatca tatctacatt ctgggcaccg
     1201 ttgacggaac catcgttacg gtttacaata caggaggcga cattgtggca ccatcaccca
     1261 caaccataaa tgctggtcag cagctctact accagctgcc tgcaggtcaa acctactaca
     1321 ggatagtggc cgacaagccg gtttacgtgt ggcatgtggg tgggtttggc tgcgagcagg
     1381 ggggggcca
//
LOCUS       DAOOQX010000596         1387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_127189_length_1387_cov_2.512012, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000596 DAOOQX010000000
VERSION     DAOOQX010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127189_length_1387_cov_2.512012"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            437..>1387
                     /locus_tag="PLX53_08735"
     CDS             437..>1387
                     /locus_tag="PLX53_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HOY73206.1"
                     /translation="MKKLLALFFLVSLIVGNAYTQELPEKNLKIQVEEVTVFIDGAQL
                     TGKATTELSKGKSLLKFTNLSPYIDAKSIRVKASNSVSVLAVNHQQNFVDRLQKPKEL
                     TDLEATLRTVAEGLKLENINLEINSEELKFMQDNRIIGGKNQETSVANFRETSSFYGT
                     KISALKLKELEINKKIEELNKKQNELQNQLNTLTSQKQYPSGEVMVNVEASNDVTATF
                     EVTFMVGNAGWFPSYDIRALSIDQPVVLVYKANIKQDSKTDWKNVKLKLSTSEPNVSG
                     VPPTLKTYFLNYNTLPPTYSQQLGRVTGRVMDSERNPLPGV"
BASE COUNT          462 a          298 c          272 g          355 t
ORIGIN      
        1 tatttttcat ttgggtagat tccaactaca taaattcaac gacagctgct tcagggtaac
       61 atccattcta tacagcaaag gtttgattag cttgcacaag tgcacatccc cgtgttggca
      121 aattccccgg ccaatagttg aaaaaattaa aaagttatca ctacatgcca ttccctccag
      181 gttttggtca cccacaggtt gatactgctg gtttcaactt ttttgcctac ctttaaagcc
      241 agctgttgca ccaatttagc tccgtataaa tcatataata tggcttcacc taaaattttc
      301 cccttcacca tcttgaaatt ctttacgaaa cggctacgtg ttttacatca acccatgtaa
      361 gcgatgaagt aaaaattctc aactaacgaa gttcagctaa atcaatttta tttaacacta
      421 ataataactt gaagcaatga aaaaactact tgccctattt tttttagtct ccttgatagt
      481 gggaaatgcc tacacccagg aactcccgga gaaaaaccta aagatacagg ttgaggaggt
      541 tacggtattc atagatggcg cccagctaac cggaaaggcc actaccgagc tttcaaaggg
      601 taaaagcttg ctgaaattta cgaatctttc accctatatt gatgcaaaaa gcattcgggt
      661 aaaggctagc aatagtgtaa gcgtgctggc ggttaaccac caacaaaatt ttgtagatag
      721 gctgcagaag ccaaaggagc taaccgatct ggaagctacc cttcggacag tagcagaggg
      781 attgaaactt gaaaacatca acctggaaat taactccgaa gagctgaagt tcatgcagga
      841 taacaggata attggcggca aaaatcagga aaccagcgta gctaacttta gagaaacatc
      901 ctcattctac ggcactaaaa tttcagctct gaagttgaag gaactcgaaa ttaacaagaa
      961 gatagaagag ctgaataaaa agcaaaatga acttcaaaac cagctgaaca ccctcaccag
     1021 ccagaaacag tatcctagtg gtgaagtgat ggttaacgtt gaagcgtcaa atgatgtaac
     1081 ggctactttt gaggtaacct tcatggttgg aaatgcaggt tggtttccct cttacgatat
     1141 cagggcttta agcatagacc aacctgttgt attggtttat aaggcaaaca taaagcaaga
     1201 ttcaaaaacc gattggaaaa atgtgaagtt gaagctatca acttccgagc caaatgtttc
     1261 aggagtaccc cctacgctaa agacttactt cttaaactac aacaccctac cgccaaccta
     1321 tagccagcaa cttggaaggg ttacgggaag ggtaatggat tctgaacgaa accccctacc
     1381 gggagta
//
LOCUS       DAOOQX010000597         1384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_127638_length_1384_cov_2.744169, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000597 DAOOQX010000000
VERSION     DAOOQX010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127638_length_1384_cov_2.744169"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..>1384
                     /locus_tag="PLX53_08740"
     CDS             172..>1384
                     /locus_tag="PLX53_08740"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOY73207.1"
                     /translation="MTSRDLAQLIAAHSRLILPNLGAFLHKDAADETFSASKVTFSPF
                     LKYNDGKLEDYLSKTTGISKEESFKQVHALVSSINQQLESSGKYPIPGLGQLVRDNQG
                     AISFVADTSVNTDTIEANFSGKISNSPPKDDGKTGEESMITGDNKASSYNSTPANRAS
                     SIKINVEADTSNPDSTAKSAIEDSINKTDSEPEKQTQDKAENIGKLENEPVKGYFKMG
                     KRSSGKKKSKLLLPALFVITSLVVIFIIALVIRHIALSPSYNYSDEDVTPENDYTPGI
                     SNMTTPKPKDKIDRAYDSLVGQIKKEESNPSSPEPSIEDKIEQEVIENYKAKDGEPTG
                     KYYLIVGSYKNADNARNMASKLKDKGFKSSVVSRPDGISCVALGAYSSNGEAEKAKLA
                     YEKQFPGIWILT"
BASE COUNT          492 a          277 c          282 g          333 t
ORIGIN      
        1 atataggtaa aattgaacag caccccggtt tgcgaataat atggataaac caacttatta
       61 tagcctattc gcaggtaata accttcattt ttttaaagag ctccttttac tccttactgt
      121 aaaatatttt taactttaca gcgctatttc aatcaaggga aaaaggtaaa aatgaccagc
      181 agggatttag ctcagcttat tgccgctcac tccaggctaa ttcttccaaa cttaggagca
      241 ttcctccaca aggatgcagc tgatgaaacg tttagcgcca gtaaagttac ctttagccca
      301 tttctaaagt ataatgatgg aaagctggag gattacctta gtaaaacaac aggcataagc
      361 aaggaggagt cgttcaaaca ggttcatgcg ctggtaagta gcattaacca gcaacttgag
      421 tcgtctggaa aataccccat tcccggttta ggacaacttg tccgcgataa ccaaggagct
      481 atttcctttg ttgcagatac atcagtaaac accgacacca tagaagctaa tttttctggt
      541 aaaatttcca attccccccc gaaagatgat ggcaaaaccg gtgaagaaag catgataacc
      601 ggggacaaca aggcttccag ctacaattcg acacctgcaa atagagcaag ttcaataaaa
      661 attaatgttg aagcagatac gagtaaccca gattcaacag caaagagtgc tatagaagat
      721 agtatcaata aaacggatag cgaaccggaa aagcaaaccc aggacaaagc tgagaatatt
      781 ggaaaattgg aaaatgaacc tgtaaaagga tattttaaaa tgggtaaaag aagtagcggt
      841 aagaagaaga gcaaactttt actaccagcc ctgtttgtga taacatcgct ggtcgtaatc
      901 tttattatcg cccttgttat tagacatata gccctaagcc caagctacaa ctactccgat
      961 gaagatgtaa ccccagaaaa cgattatacc ccgggtattt ctaacatgac aaccccaaaa
     1021 ccaaaggata aaattgacag ggcatacgat tccctagtgg gccaaatcaa aaaggaagag
     1081 agtaaccctt caagccctga gcctagcatt gaggacaaaa ttgagcagga agttattgaa
     1141 aactacaagg ctaaagatgg cgagccaaca ggtaaatact acctgatagt tggcagctat
     1201 aaaaatgccg ataatgctcg gaatatggct agtaagctta aagataaagg atttaaaagt
     1261 tcggttgttt caaggcctga cggtataagc tgtgttgccc taggagcata ttcatcaaac
     1321 ggtgaagcgg aaaaagccaa gctagcctat gaaaaacaat ttcctggaat atggattttg
     1381 accg
//
LOCUS       DAOOQX010000598         1383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_127732_length_1383_cov_4.029367, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000598 DAOOQX010000000
VERSION     DAOOQX010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127732_length_1383_cov_4.029367"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="PLX53_08745"
     CDS             <1..947
                     /locus_tag="PLX53_08745"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOY73208.1"
                     /translation="WAAGAMLARVYLFHKGYGKGVLGITSDLTVDNVPITESNIEQIV
                     DDIINLSGHALVPNFADLWGVNHENNKESIFEIQHTDKSNWGDWGWRNGTEGNWTVVM
                     SGFRDVSDPIYDLGWGFQPASQSLANEFEPGDPRFSVSILDAAAENLVYKPQNCYQHT
                     GYAFKKYYPKKADKPLSNPDLNWGYNRVVIRLADVLLMGAELYLDNDLLKAQGYYEQV
                     RKRAFGQSHVAPTLTNDQNGLDLIYHERRVEFAGEGIRYWDLLRRGLNYAQQKIDAAS
                     SASPYDETFKPATKGLLPIPQSEISISGNILVQNEGYI"
     gene            972..>1383
                     /locus_tag="PLX53_08750"
     CDS             972..>1383
                     /locus_tag="PLX53_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73209.1"
                     /translation="MSKKNILFGVLVLVGLTWHACEPSEVGKPDVGKAPRVEDLSFTI
                     TPGEDAFHFVFTNTSSLKGIANWDLGNGSKAIGNTVTAYFPIAKTYTVTLTLYASGGS
                     ASLSQELTQNETDWDFLASPLITAITGGVESANGK"
BASE COUNT          375 a          312 c          326 g          370 t
ORIGIN      
        1 aatgggctgc cggagcaatg ctcgctcgag tttacctctt ccacaaggga tatggaaaag
       61 gtgttcttgg gattacttct gatttaactg ttgacaatgt ccccataacc gaatcgaata
      121 ttgagcagat tgttgatgac ataattaatc taagcggcca tgcccttgtt cctaactttg
      181 ccgatttgtg gggagtaaat catgaaaata acaaggagtc catattcgaa attcaacaca
      241 ccgataaaag caactggggt gattggggct ggagaaatgg aactgaaggt aactggaccg
      301 ttgtaatgtc gggatttcgt gatgtatccg accctattta cgatttgggt tggggctttc
      361 agcctgcctc ccagtctctg gctaatgaat ttgaacctgg cgatccacgc ttctccgtta
      421 gcattttgga tgcagctgct gaaaatctcg tttataagcc tcagaattgc taccagcata
      481 ccggctacgc ctttaaaaag tactatccaa agaaagccga taaaccgttg tcgaatcccg
      541 acttaaactg gggctacaac agggtggtaa ttcgccttgc cgacgttctg ctaatgggag
      601 ctgagctata cctggataac gatcttctta aggcacaggg atactacgaa caggttcgga
      661 agcgtgcttt tggccagtcg catgttgccc caactttaac caacgaccag aacggccttg
      721 accttatcta ccatgaacgt agggtagagt ttgccggcga aggcattaga tactgggatt
      781 tactacgccg tggtttaaac tatgcacagc aaaaaattga tgctgcaagc agtgcaagcc
      841 cctacgacga aacatttaaa cctgcaacca agggattact gcccattcca cagtcggaaa
      901 tctccatttc gggtaatatc ctggtgcaga atgaggggta catttaattt attaacaata
      961 atctaatagc tatgtcgaaa aaaaatatac tattcggagt tctggttcta gttggcctta
     1021 cgtggcatgc ctgcgaacca tctgaagttg gtaaacctga tgttggcaaa gcacctaggg
     1081 ttgaagacct gagctttacc attacccccg gcgaagatgc atttcacttt gtttttacca
     1141 acacctcttc gttgaaggga attgcaaact gggatttggg aaatggttct aaggctatcg
     1201 ggaatactgt tactgcctat ttccctattg ccaaaaccta cacagttacg cttaccctgt
     1261 acgcttcggg gggaagcgcc tcgctttcgc aggagttgac tcaaaatgag acagactggg
     1321 acttccttgc tagcccgctg attactgcta taacaggggg cgttgagtct gccaacggca
     1381 aaa
//
LOCUS       DAOOQX010000599         1383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_127790_length_1383_cov_2.478916, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000599 DAOOQX010000000
VERSION     DAOOQX010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127790_length_1383_cov_2.478916"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..458)
                     /locus_tag="PLX53_08755"
     CDS             complement(222..458)
                     /locus_tag="PLX53_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73210.1"
                     /translation="MPFFNSTMVRLKGGNFNEIRKGETNKLFVIVKNGMTEVAVIRIN
                     KENGCYIVEKASVMRKAYVNRKELLWQRERTLLT"
     gene            complement(777..>1383)
                     /locus_tag="PLX53_08760"
     CDS             complement(777..>1383)
                     /locus_tag="PLX53_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY73211.1"
                     /translation="LHYHKDNFIVDVAGFYNIVNSYIFISPTGDTTASGIYIFSYKQA
                     NSILFGGEAGLHVHPKSLKWLHLETTFSSVIGKQRNDLYLPFVPANKLRFELRGEKEK
                     LALINNAFASISINTAFKQNNAAPDETPTAGYTLIDLSIGGQIKVQNQLISFSISANN
                     LTDKKYIDHLSTLKEVDLYNPGRNISLSIHIPFELKASSEN"
BASE COUNT          400 a          293 c          280 g          410 t
ORIGIN      
        1 ccttttaacc gaaccatagt ggaattgaaa ttgtacattc aacggctaaa agtctaaaac
       61 tatggcactt ttaaccgaac catagtggaa ttgaaatttt tgactttaac cttaaaatgg
      121 tttacctttg tatttaggag ttgttgtgtg cttctaagcg ttctgttatc gctgcctgcc
      181 caaataagca gcatagcagg tcaaagaaac ctgagggcag ttcaggttaa tagggttcgc
      241 tcccgttgcc acagcaactc ttttctgttt acatatgctt tccgcatcac acttgccttt
      301 tcaacaatgt agcatccgtt ttccttgttt atccgaataa cggcaacctc tgtcatccca
      361 ttcttaacta ttacaaacaa cttatttgtc tcccctttcc ttatttcgtt aaagttaccc
      421 ccttttaacc gaaccatagt ggaattgaaa aaaggcacca ctagttatgg tgaaaaaagc
      481 taaacccggt ggtgccacga tatcaacaag gaatacattt acgcccgcca ttagggtcgg
      541 tgccccacag gaaaaccaca gccgtgctat acggtaagcc ggcataaaaa aatgggaaaa
      601 aggaagaatg gagctaccag gagcaggaag gggtgccaac agcccgagta gccctgcgag
      661 ctatttcggg ttattaacac cccatcagca tcaggcctta aagctaaaat tcagccagga
      721 aatggctata agcgttccta aaaatgggga gagtcatagc ccctttttag tagctgttaa
      781 ttctcactac ttgcttttaa ctcgaatgga atatgaatgc ttaacgaaat attccgaccg
      841 ggattgtata aatccacttc tttcagtgtt gaaagatggt caatgtattt cttgtccgtt
      901 aggttgtttg cgcttatgct aaatgaaata agctgattct gcactttaat ttggccacca
      961 atgcttaaat caataagcgt atatccagca gtaggcgttt cgtcaggcgc agcgttgttt
     1021 tgcttaaatg cggtgtttat gcttatagag gcaaatgcat tattaataag cgctaatttt
     1081 tccttttcac cccgtaattc aaaccgtaat ttattagctg gcacaaatgg caggtagagg
     1141 tcattccgtt gcttgccaat tacggatgaa aacgtggttt caaggtgcag ccattttaac
     1201 gatttgggat gaacatgcaa acctgcttcg cctccaaaca atatggaatt tgcctgcttg
     1261 tagctaaaaa tatatatccc cgaggcagtt gtgtcgcctg ttggtgaaat aaatatgtag
     1321 ctattgacaa tgttgtaaaa acctgccaca tcaacgatga agttatcctt atggtaatgt
     1381 agg
//
LOCUS       DAOOQX010000600         1379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_128444_length_1379_cov_2.530211, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000600 DAOOQX010000000
VERSION     DAOOQX010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128444_length_1379_cov_2.530211"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLX53_08765"
     CDS             <1..759
                     /locus_tag="PLX53_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOY73212.1"
                     /translation="QLDELTDFVKRPQVGASGLVYIRYNDDGTLKSSVDKFYSADDLM
                     LVAKVFEAKPGDLMLILAGKRNKTLPALSELRLEMGNRLGLRPKNQFAPLWVVDFPLL
                     EWDEELQRFFAMHHPFTSPHPDDIPLFQSNPAAIRANAYDMVINGVEVGGGSIRISNR
                     ELQQTMFNTLGFSDDEAEKQFGFLLNAFRYGAPPHGGIAFGFDRLCSLFGGSDSIRDY
                     IAFPKNNAGRDVMIDSPSSISQQQLDELNIIIKE"
     gene            complement(846..1031)
                     /locus_tag="PLX53_08770"
     CDS             complement(846..1031)
                     /locus_tag="PLX53_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73213.1"
                     /translation="MKKIALFVIAAGMISMVACKSAPKQEEATEQPATEQTQQETAQP
                     DSAAQAAETPAQPAPAQ"
     gene            complement(1118..>1379)
                     /locus_tag="PLX53_08775"
     CDS             complement(1118..>1379)
                     /locus_tag="PLX53_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73214.1"
                     /translation="TLGVGKLVGMPIAGTMTAVWWESQVDPTLVFGIPQMGVKDLNGK
                     YLENQQLNPDIKVTISPEEAQSGVDSQLIKAVEELLLELNQK"
BASE COUNT          349 a          313 c          314 g          403 t
ORIGIN      
        1 cagctcgatg agctgaccga ctttgtcaaa cggccacagg ttggagccag cggtttggtt
       61 tatattcgct acaacgatga tgggacactg aaatcgtcgg ttgacaagtt ttactctgcc
      121 gacgatttga tgctggttgc caaagtgttt gaagccaaac ctggcgactt gatgctgatt
      181 ttggcaggca agcgcaacaa aacccttcct gcacttagcg agttaaggct cgaaatggga
      241 aaccgcttgg ggttacgccc taaaaatcag tttgctcccc tatgggtagt tgacttccct
      301 ttactcgaat gggacgagga gttacagcgg ttttttgcca tgcaccatcc ctttacttcg
      361 ccccatcccg atgatatacc cttattccaa tcaaatcctg cggccatacg ggccaacgcc
      421 tatgatatgg ttatcaacgg agttgaggta ggtggtggat ccattagaat ttccaacagg
      481 gagctgcagc aaaccatgtt taacacgttg ggtttttccg atgatgaagc ggaaaaacaa
      541 tttgggtttc ttttaaatgc cttcaggtac ggcgctccgc cccacggagg aatagcgttt
      601 gggttcgatc gcctctgctc cctttttgga ggctccgatt ccatcaggga ttatattgcc
      661 tttccgaaga ataatgcggg ccgggatgtg atgattgact cgccttcatc cattagccaa
      721 cagcagcttg atgagctgaa tatcatcatt aaggagtagt ttttactggc actacccttt
      781 ttttaaaaaa taaaacaccc cacattaaaa atatggggtg tctttatttg tccttatagc
      841 aacaactact gtgcaggagc aggttgagca ggagtttcgg cagcttgtgc tgcagaatcg
      901 ggctgagctg tttcttgctg agtttgttca gtagcaggtt gttcagtagc ctcttcctgt
      961 tttggagcag acttgcatgc aaccatggaa atcattccgg cggcaattac aaataaagca
     1021 atctttttca tagtagtata gatttagtta gtttgtacaa ttacaaaggt ataaataata
     1081 tcatttacta caaaaatttc catgacatta ttgagtccta tttttggttg agctctaaaa
     1141 gcagctcttc aaccgctttt atgagctggc tatctacgcc cgactgggct tcctccggtg
     1201 aaatggtaac tttaatatcg ggattcagct gttgattttc cagatacttg ccgtttaaat
     1261 ccttaacacc catttgtgga atcccaaaaa ccagggtggg gtcaacttgc gattcccacc
     1321 aaactgcagt catggttccg gctataggca taccaaccag cttcccaacg cctagagtt
//
LOCUS       DAOOQX010000601         1378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_128597_length_1378_cov_2.675737, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000601 DAOOQX010000000
VERSION     DAOOQX010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128597_length_1378_cov_2.675737"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..>777
                     /locus_tag="PLX53_08780"
     CDS             52..>777
                     /locus_tag="PLX53_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOY73215.1"
                     /translation="MKSFIQTLKNIFKIEDLRKRILYTLGILLVYRLGSFIVIPGLDP
                     DQLSALQEQTKEGIMGLLNMFSGGAFSNASIFALGIMPYISASIVVQLLGIAIPYFQR
                     LQKEGESGRNKLNQITRILTIAILILQAPAYLANLHAQLPESAFIVKGASFTIFATIV
                     LIAGTMFIMWLGEKITDKGIGNGISLIIMIGIIARLPFALVAEFFSRAEELGGGLVMF
                     LAELVVLFVVFMFTILLVQGTRKI"
     assembly_gap    778..787
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <788..>1378
                     /locus_tag="PLX53_08785"
     CDS             <788..>1378
                     /locus_tag="PLX53_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HOY73216.1"
                     /translation="GNKQYGGVRQYIPLKVNAAGVMPIIFAQAIMFIPMLFARFDSTA
                     GIAAAFNYTGFLYNFTFAILIILFTYFYTAIIINPNQMAEDMKKNGGFIPGVKPGKKT
                     VEFLDSIMSKITLPGSIFLAIIAILPAFAMILGVNNQFAHFFGGTSLLILVGVVLDTL
                     QQIESHLLMRHYDALTKSGRVKGRAGYSLMPAPTFFT"
BASE COUNT          338 a          302 c          297 g          431 t
ORIGIN      
        1 caatcgaggc tatcgaatca gccaacggaa cggctgttaa aatttaaatg gatgaaatcc
       61 tttattcaaa cacttaagaa tattttcaag attgaggacc ttcgaaagag gatcctctat
      121 actcttggaa ttctcttggt ttacaggctt ggtagtttta ttgtaattcc agggttagac
      181 cctgaccagc tttctgctct gcaggaacaa accaaggaag gaatcatggg gcttctgaac
      241 atgttctcgg gtggtgcttt ttctaatgca tccatatttg ccctgggtat tatgccctac
      301 atttcagcct ccattgtggt gcagctactt ggaattgcaa taccctactt tcaacggtta
      361 cagaaggagg gagaaagcgg tcgtaacaag ctaaaccaaa ttacccgaat tcttaccatt
      421 gctattctta ttcttcaggc tcctgcctac ctggcaaacc ttcatgccca gcttcctgag
      481 tctgccttta ttgttaaagg tgctagcttt accatatttg ctaccattgt gctgattgcc
      541 ggaaccatgt ttatcatgtg gcttggcgaa aagattaccg ataagggtat tggcaacggg
      601 atttcactta taatcatgat tgggattatt gcccgacttc ctttcgcact ggttgctgag
      661 ttcttctcaa gggccgagga gcttggtgga ggtttggtta tgttccttgc cgagctggta
      721 gtgctttttg ttgtattcat gtttacaatc ttgcttgttc agggaacccg taaaattnnn
      781 nnnnnnnggc aacaagcagt acggtggcgt tagacaatac attcccctta aggtgaacgc
      841 agcaggcgtt atgcctataa tttttgccca ggccatcatg ttcattccca tgctgtttgc
      901 ccggtttgac tccactgcag gaattgctgc tgcttttaac tataccggct tcctttacaa
      961 ctttaccttt gcaattttaa tcatcctttt cacctatttc tacacggcaa ttatcatcaa
     1021 tcccaaccag atggctgagg atatgaaaaa aaatggtgga tttattccag gcgttaaacc
     1081 aggaaagaaa acagtggagt ttttggattc catcatgtcc aagattaccc ttccgggttc
     1141 aattttcctg gccataatag ccattcttcc tgcatttgcg atgatacttg gggtaaacaa
     1201 ccagtttgca cacttttttg ggggcacatc gctgttaatt cttgtaggag ttgttctgga
     1261 tacactacag caaattgaaa gccatttgct gatgcgacac tacgacgcac tgacgaagtc
     1321 tggtcgggtt aagggaaggg caggttactc gttgatgcct gcacctacgt tctttaca
//
LOCUS       DAOOQX010000602         1373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_129322_length_1373_cov_4.544006, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000602 DAOOQX010000000
VERSION     DAOOQX010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129322_length_1373_cov_4.544006"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLX53_08790"
     CDS             <1..287
                     /locus_tag="PLX53_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HOY73217.1"
                     /translation="AYVSSLVQTLTFSGDINLTPKWKIAFSSGFDLKSRQLTTTSLNF
                     YRDLHCWEMRLTVIPIGYYKSFSFQINVRSSILQDLKLTKRDHYLDNLGE"
     gene            369..746
                     /locus_tag="PLX53_08795"
     CDS             369..746
                     /locus_tag="PLX53_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOY73218.1"
                     /translation="MKKIINTNDAPRAIGPYSQAVEANGFLFVSGQVPVNPSTGKIVE
                     GGIAEQTEQVVKNIGAILAKAGLTYANVVKTTCLLSDMENFATMNEVYARYFTAEMPA
                     RAAFGVVKLPLGALVEIECIAIR"
     gene            complement(795..1241)
                     /gene="rplI"
                     /locus_tag="PLX53_08800"
     CDS             complement(795..1241)
                     /gene="rplI"
                     /locus_tag="PLX53_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOY73219.1"
                     /translation="MEIILLQDMPSLGSKNDIINVRNGYARNYLIPKGFATIATESAK
                     KVLAENTKQRAHKEAKIKADAEELAKKLNGLKLTIGAKTSSTGKIFGSVNTIQISEAL
                     AEKGFEIDRKLIFIQDDQIKEVGSYTAEVKLHKEVKVSIEFDVVSE"
     gene            complement(1263..>1373)
                     /gene="rpsR"
                     /locus_tag="PLX53_08805"
     CDS             complement(1263..>1373)
                     /gene="rpsR"
                     /locus_tag="PLX53_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOY73220.1"
                     /translation="PRRLTGTSQKYQRKVSTAIKRARHLALLPFVTDLMK"
BASE COUNT          340 a          317 c          311 g          405 t
ORIGIN      
        1 ctgcctatgt atcctcctta gttcagaccc tgaccttttc gggcgatatc aacctgactc
       61 caaaatggaa aattgccttt agctccgggt tcgatttgaa gagccggcag cttacaacca
      121 cgtccctcaa tttctacaga gacctgcact gctgggagat gcggctgacc gttataccta
      181 ttggatacta caaaagcttc agctttcaga taaacgttcg ctcctccata cttcaggatt
      241 taaagcttac caagcgcgac cactacctcg ataacctggg cgaataaccg ggctgatttt
      301 cttgattgct tactagcatg ataaattttt gccgaaatct atatttatta cactaaaatt
      361 tgttaatcat gaaaaagatt atcaacacaa atgatgctcc tcgggcaatt ggcccataca
      421 gccaggctgt tgaggccaat gggtttcttt ttgtgtcggg gcaggttccc gtgaatccat
      481 caaccggtaa aattgtagag ggaggaattg ctgagcaaac cgagcaggta gttaaaaaca
      541 taggggcaat tctggcgaag gccggcctta cctatgccaa cgtggtgaaa actacctgcc
      601 tgcttagcga tatggagaat tttgccacca tgaacgaggt atatgctcgt tactttacag
      661 ctgaaatgcc ggcaagggct gcctttgggg ttgttaaact cccgcttgga gccttggtgg
      721 agattgagtg catagccata aggtagaaaa aaaaacctgc caacggcagg tttttttatg
      781 ctggatgagt agtgctattc cgatactaca tcaaattcga tgctgacctt cacctcctta
      841 tgcagcttta cctcggcggt gtaggagcca acctctttaa tttggtcgtc ctgaatgaag
      901 attagcttgc ggtcaatttc aaatcctttc tcggcaagcg cctccgaaat ctgaatggtg
      961 ttcaccgaac caaaaatttt accggtcgag ctggttttag ctccaatggt aagcttcagg
     1021 ccgttaagct ttttagccag ctcttcggca tcggctttaa tttttgcctc cttatgggct
     1081 cgctgcttgg tgttctcggc cagcactttt ttggccgatt ctgtggcaat ggtggcaaag
     1141 cctttaggaa taagatagtt tcgagcgtaa ccgtttcgaa cgtttataat atcgttttta
     1201 cttcccaaac tgggcatgtc ctgtaatagt atgatttcca tttgcttatc tcctaccttt
     1261 aattatttca tcaaatcggt tacaaatggc agtagggcta aatgccgagc ccgcttaatg
     1321 gcagtagata cctttcgttg gtacttttgc gaggttccgg ttaaccgccg ggg
//
LOCUS       DAOOQX010000603         1369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_129984_length_1369_cov_3.156773, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000603 DAOOQX010000000
VERSION     DAOOQX010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129984_length_1369_cov_3.156773"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="PLX53_08810"
     CDS             complement(<1..1148)
                     /locus_tag="PLX53_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73221.1"
                     /translation="MNHNKGKWCRIGSMVLLTALLTGCYDFSNFENFKVEPVNPSIVA
                     PVVNSTITFKELAETEDANTIVIVKPGDTRFYLSFRDTMEIANASGEFSIPPFAYSKT
                     YQPDAGEIPPVAPPAGESITFSPKTFTQTWQPDADSELKLIKLSQGSMNITVTNNFGN
                     IISGSITLNFLLTPQNQPYTINIPNINPASSQAISLTNLAGYSFNLFDGTSYNTLTFT
                     ATITLTSMGNSINPGDNINIDISANNLDFTEIYGKVNKTINLSDQSFPLDIFRSTYIA
                     DQHLAEPSLMLKFANSYGIPLSLNVNQLVASNDQTGSMNVVNETVPPDGSLLIGAPNS
                     LNYVGSPSQEFAMDSLKLDYQNSNIEDVFDFAPNNFTLKSGITLGDAST"
BASE COUNT          356 a          226 c          371 g          416 t
ORIGIN      
        1 gttgaggcat cgccaagggt aattcccgat ttaagggtaa aattgttagg ggcaaagtcg
       61 aaaacatcct caatgttgga gttttgatag tccagcttta gggagtccat tgcaaattcc
      121 tgggatgggc tcccaacata gtttaacgaa ttgggtgcac caatgagtag tgacccatcg
      181 ggaggaacag tttcgttaac cacgttcatg gatccagttt ggtcgttgct ggctaccagc
      241 tggttaacgt taagcgacag gggaatcccg tagctgttgg cgaactttaa cataaggctg
      301 ggctcggcca ggtgctggtc ggcaatgtag gtggaacgga atatatccaa cggaaaagat
      361 tggtcggaaa ggttgatggt tttattgacc ttgccgtaaa tttcggtgaa gtcgagattg
      421 tttgccgaaa tgtcgatatt gatgttatcg ccagggttta tagaattgcc catagatgtg
      481 agggtgatgg tagcggtaaa tgtaagggtg ttgtagctgg tgccgtcgaa aaggttaaag
      541 ctgtagcccg ccaggtttgt tagcgaaatg gcttgacttg aagcgggatt gatattggga
      601 atgttgatag tgtagggttg attctgaggg gttagtagaa agtttaacgt gatgctgccg
      661 ctaatgatgt ttccgaagtt gttggtcacg gtaatgttca tacttccctg agatagcttg
      721 atgagtttta gctcagaatc ggcgtcaggt tgccaggttt gggtgaaggt tttggggctg
      781 aaggtaatac tttcgccagc tggaggtgca accggaggaa tttcacctgc atcgggttga
      841 taggttttgg agtaggcaaa tggtggaatt gaaaactcac cactggcatt ggcaatttcc
      901 atggtatcgc ggaaggagag gtaaaacctg gtatcgcctg gtttaacaat cacaatggta
      961 ttggcgtctt cagtttcggc cagctcctta aaagttatag tggagttaac caccggagca
     1021 actatggagg ggtttacagg ttcaaccttg aaattttcaa aatttgaaaa atcgtagcat
     1081 cctgtaagta gtgctgtaag caaaaccatg ctgcctattc tgcaccattt acctttattg
     1141 tggttcataa ccagcgtttt tgtttatgaa attttggtga aatttagcaa tatttaaatc
     1201 acaattcaaa attttagtaa acacattctc atagattttg tatttatgcc tgataatgtt
     1261 ctatgagtgg ctgttgtcta gtcagctttt atgatttgtt atgctctgat gagtagtgcg
     1321 ataaggcgct gaaattgaac accttgttgc ccgatgatca aagatatta
//
LOCUS       DAOOQX010000604         1368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_130144_length_1368_cov_3.047982, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000604 DAOOQX010000000
VERSION     DAOOQX010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130144_length_1368_cov_3.047982"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..983
                     /locus_tag="PLX53_08815"
     CDS             270..983
                     /locus_tag="PLX53_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOY73222.1"
                     /translation="MEWIERTELLLGAAKLQKLSRAHVLVVGLGGVGAYAAEMICRAG
                     VGQLTIADGDTIHPTNINRQLVALNSTVGLPKAEYMGKRLLDINPDIKLMVVPKYLRD
                     DEMKDIINQPYSYVVDAIDTLSPKIFLIRDALEYGHRLVSSMGSGGKLDPTMVSIADI
                     SETHSCPLARILRKRLHRLGIRKGFKVVYSPEETPENATRPCVGEPNKKTTVGTISYM
                     PAVFGCFIASVVIRDLLAD"
     gene            1144..>1368
                     /locus_tag="PLX53_08820"
     CDS             1144..>1368
                     /locus_tag="PLX53_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY73223.1"
                     /translation="MISVNQLTLSFGGFELFKEISFQLNEGERVGLVGRNGAGKTTLL
                     KIIAGEMQPTSGRVSMPGSVSIGYLPQQMAL"
BASE COUNT          360 a          352 c          328 g          328 t
ORIGIN      
        1 agctggccga atcgcttacc gttatcccca aaaacaggct gttttttgaa accgatacct
       61 ccgaaatgcc tattggggag atatactccg ccgctgcaga tatcctcgac acctcagttg
      121 atgagcttac cctgcaagtt gagaataacc tgaatacgtt tttcgggttt aatggctaat
      181 tgccatgtaa aattacccgg ctaaaacacc ccttgcctca cacccgagga aatgtagcaa
      241 ctttatgagc actctaccaa cttacagcaa tggaatggat tgagcgaacc gaactcctgc
      301 ttggagctgc taaactgcag aagttaagcc gggcgcatgt gctggtggtt gggcttggcg
      361 gtgttggcgc ctatgctgcc gaaatgattt gtcgcgccgg agttgggcag cttacaatag
      421 ccgatggcga taccatccac cctaccaaca tcaacaggca gctggttgcc ctgaacagca
      481 cggttggcct gcccaaagca gaatacatgg gaaaacgcct gctggatata aaccctgata
      541 ttaagctcat ggtggtgcct aagtacctgc gcgacgatga gatgaaggac atcatcaatc
      601 agccctacag ctacgtggtg gatgccattg acaccctctc acctaaaata ttcctgattc
      661 gcgacgccct ggagtatgga caccgccttg taagctcaat gggtagcggt ggaaaacttg
      721 atccaaccat ggtttccatt gccgatattt cggaaaccca cagctgcccg cttgcccgaa
      781 ttcttcgcaa acggctccac aggttgggta tccgtaaggg atttaaggtg gtttactccc
      841 ctgaggaaac gcccgaaaac gccacaagac catgtgtggg tgaaccaaat aagaaaacta
      901 ctgttggcac aatctcttac atgcctgctg tttttgggtg ctttattgct tcggtggtga
      961 tacgtgacct actggccgac taacccacaa tggttgtggt taacgcaatc actcctcctc
     1021 catcaacatc atcacaactg cacatccaca gcttcacggt ctggatggca caataatgcc
     1081 attgatggca gggtgaagcg aaattagtat ctttgcagcc aataactttt cgagtagtct
     1141 cagatgatat cagtaaacca gctaaccctc agcttcggag gttttgaact tttcaaggaa
     1201 atcagctttc agcttaacga gggcgaacgg gttgggttgg ttggccgaaa cggcgcagga
     1261 aaaactaccc tactaaagat tattgcaggc gaaatgcagc caacctcagg acgcgtttcg
     1321 atgccaggca gcgttagcat tggatacctc ccccagcaaa tggcattg
//
LOCUS       DAOOQX010000605         1365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_130660_length_1365_cov_2.383206, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000605 DAOOQX010000000
VERSION     DAOOQX010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130660_length_1365_cov_2.383206"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1365)
                     /locus_tag="PLX53_08825"
     CDS             complement(<1..>1365)
                     /locus_tag="PLX53_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOY73224.1"
                     /translation="IEVDVQRIGGGFGGKEDQPSQFAALAALATHLLKQPVKLSLSRH
                     DDMRMTGKRHPYSADFKIGLDKNLKIIAYETTLYQNGGASADLSPAIMERTLFHSTAS
                     YFIPNTRVTVYSCRTNLPPNTAFRGFGAPQGMFVMESAIAKAAHELGVAAQTIQELNL
                     ISEKDEFQYGQVAENAYATKCFERAKEQFKIEVLQKEIKLYNSKNRFSKRGMAVMPLC
                     FGISFTNITMNQARALVHIYQDGSVSVSTGAVEMGQGVNTKLAQVAAITLGIGIERVK
                     IYSTNTSRVANTSPTAASTGADINGNALLIAATRLLERLKQVAAEMLSCRASEVEIKN
                     EEVLCRGERSSTSWQKLIQTAYLKRICLTENGHYSTPKIYFDKTKEKGHPFAYMVYGT
                     GIVTTTVDCIRGTYSIDEVLVVHDFGNSLNPIIDTGQLEGAIVQGIGWATIEELMFNN
                     KGTLL"
BASE COUNT          297 a          335 c          330 g          403 t
ORIGIN      
        1 aggagggttc ctttgttgtt gaacataagc tcttcaatgg tagcccatcc aattccctga
       61 acaatagcgc cttcaagctg tccggtatct ataatggggt taaggctatt gccaaaatcg
      121 tgaactacca gcacctcgtc aatagaatat gtgcccctga tacagtcaac ggtggtagta
      181 acgatgcctg tcccgtaaac catgtaggca aaaggatggc ctttctcctt ggtcttgtca
      241 aagtatattt ttggggtaga gtagtgcccg ttttcggtga ggcatatcct tttcaggtag
      301 gcagtttgaa tcaacttttg ccaggatgta ctgctgcgct ctccccgaca taaaacttcc
      361 tcatttttaa tttccacctc gctggccctg cagctgagca tttctgcggc cacctgcttt
      421 agcctttcaa gaagccgggt tgcagctatc agcagggcat ttccattgat atcggcacct
      481 gtacttgcgg cggtaggcga cgtgttggca accctggatg tgttggtaga gtatattttc
      541 accctttcaa ttcctatgcc taaagtaatg gcagcaacct gggcgagttt ggtatttaca
      601 ccctgtccca tctctacagc tcctgtgcta accgatacgc ttccatcctg gtatatatgc
      661 accaaggccc ttgcctggtt cattgttatg ttggtaaatg atatgccgaa gcacaggggc
      721 atgacggcca tgcctcgctt gctgaaccta tttttgctgt tgtaaagttt aatttccttt
      781 tggagcactt cgattttgaa ctgttctttt gccctttcaa agcattttgt agcataagcg
      841 ttctcggcca cctgtccata ctgaaactcg tctttttcac taatcaggtt cagctcctgt
      901 atggtttgtg ctgctacacc cagctcgtga gcagctttag caatggccga ttccattaca
      961 aacattccct gaggtgcccc aaacccacga aatgctgtgt tgggtggcag gtttgtccgg
     1021 cagctgtaaa cggttacccg cgtattgggg ataaagtagg aggcagttga atggaatagc
     1081 gttcgctcca tgatggcggg tgatagatcg gccgaagccc caccgttctg gtaaagagtg
     1141 gtttcgtagg ctataatttt aaggtttttg tcgagtccaa ttttaaaatc ggcgctgtag
     1201 ggatgtcttt tccctgtcat ccgcatgtcg tcgtggcgcg agagcgaaag ctttacaggc
     1261 tgcttaagca ggtgtgtggc cagtgctgcc agtgctgcaa actgcgaagg ctgatcctcc
     1321 ttgccgccga atccacctcc aatgcgttga acgtcaactt caatc
//
LOCUS       DAOOQX010000606         1364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_130741_length_1364_cov_3.320856, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000606 DAOOQX010000000
VERSION     DAOOQX010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130741_length_1364_cov_3.320856"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PLX53_08830"
     CDS             <1..654
                     /locus_tag="PLX53_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOY73225.1"
                     /translation="ISYAGQPSPDGLAQAFIIGENFIGSDSVCLVLGDNIFYGGNLTE
                     MLLESAKLQDGAIVFGYNVKDPQRYGVVGFDSDGKVNSLEEKPTNPKSNYAVTGLYFY
                     SNDVVAKAKGLKPSKRGELEITDLNRLYLEEGRLKVKLMGRGMAWLDTGTHESLLQAS
                     IFIQTIEERQGLKIACLEEIAYRNGYINLEQLRKLATPLENNQYGEYLKRLASGNFS"
     gene            694..1263
                     /gene="rfbC"
                     /locus_tag="PLX53_08835"
     CDS             694..1263
                     /gene="rfbC"
                     /locus_tag="PLX53_08835"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOY73226.1"
                     /translation="MLTVEKTSIPDLLILKPNIFEDSRGYFFESFNAKLFDDFAITSN
                     FVQDNESFSKYGVIRGLHYQLSPYSQTKLVRVVYGKILDIALDIRRNSPTFGRYVAIE
                     ISAENKAQFYIPKGFAHGFSVLSDSAIVCYKCDAYYSKEAERGIRFNDPGLKIDWGIP
                     DGSMVVSEKDLKLPFFSEADINFNYGSIT"
BASE COUNT          374 a          305 c          328 g          357 t
ORIGIN      
        1 atcagctatg ccgggcagcc ctcacccgat gggcttgcac aggctttcat cattggcgaa
       61 aactttattg gctccgacag cgtttgcctg gtcctgggcg ataatatttt ctatggcgga
      121 aaccttactg aaatgctcct cgaaagtgcc aaacttcagg atggagctat tgtttttggc
      181 tacaacgtta aggatcccca gcgctatggt gttgtgggtt tcgatagcga cggaaaagtg
      241 aatagcctgg aggaaaaacc caccaaccca aagtcgaact acgccgtaac agggctttac
      301 ttctacagca acgatgtggt ggccaaagct aaaggtttaa aaccttcaaa acggggcgag
      361 ctggaaataa ccgacctgaa caggctttac ctcgaagagg gcaggctaaa ggttaagctg
      421 atgggacgcg gtatggcctg gctcgacacc ggtacgcacg aaagcctgct gcaagcctcc
      481 atttttattc agaccattga ggaacgccag gggcttaaaa ttgcctgtct ggaggaaata
      541 gcttatcgca acggctacat caacctggag caactccgga aattggcgac gcctctggag
      601 aacaaccagt acggtgagta cctcaagagg ctggcttctg gtaatttttc ctaaccgcta
      661 acccgtttct tttttaacga aagctgataa gtgatgctca cagtcgaaaa aacctcaata
      721 cccgatctac tgatacttaa acccaatatt tttgaggatt ccagaggata tttttttgaa
      781 agctttaacg ccaagctatt tgatgatttt gccattactt ctaattttgt tcaggacaac
      841 gagtcgtttt cgaagtatgg cgtcatcagg gggttacact accagctgtc accctactcc
      901 caaacaaagc tggtaagagt ggtttacgga aaaattttgg atattgctct ggatataagg
      961 agaaattctc ccacttttgg caggtatgta gctatagaaa tctctgctga gaacaaggca
     1021 cagttttaca ttccaaaagg atttgcacat ggtttttcag tgctgagcga ttcagctata
     1081 gtttgctaca agtgcgatgc ctactacagt aaagaagcgg agaggggaat acgcttcaac
     1141 gatccgggtt tgaagattga ctggggcatt cccgatggta gcatggttgt ttcggaaaag
     1201 gacctcaagc ttcccttttt ctctgaagca gatattaatt ttaattatgg cagcatcacc
     1261 tagcagggtt ttggttacag gtgcaaacgg gcagcttgga accgctcttc gtaaacgggc
     1321 aaccaagccg gcgaatcatc tcgtagctac atttacagac ttta
//
LOCUS       DAOOQX010000607         1364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_130837_length_1364_cov_1.954163, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000607 DAOOQX010000000
VERSION     DAOOQX010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130837_length_1364_cov_1.954163"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="PLX53_08840"
     CDS             complement(<1..107)
                     /locus_tag="PLX53_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOY73227.1"
                     /translation="MADLKKFAEDLVNLSVKEVNELAKILKDEYGIEPAA"
     gene            complement(142..666)
                     /gene="rplJ"
                     /locus_tag="PLX53_08845"
     CDS             complement(142..666)
                     /gene="rplJ"
                     /locus_tag="PLX53_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOY73228.1"
                     /translation="MTREEKKNLIDALTETINRYPHFYLTDTSALNAEDTSKLRRSCF
                     QKEVKMVVVKNTLFKKALEQSGKFDFSPLFTELKGTTSVMFTESGNVPAKLIKEFRTE
                     HPKPLLKAAFVEESIYVGDEQLNMLASLKSKNELIADVIALLQSPAKNVIASLQSGGN
                     ILSGVVKTLSEKEK"
     gene            complement(694..1311)
                     /gene="rplA"
                     /locus_tag="PLX53_08850"
     CDS             complement(694..1311)
                     /gene="rplA"
                     /locus_tag="PLX53_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOY73229.1"
                     /translation="MLKDITYTKFDASVDIDVRLNVDPRKANQMVRGVVTLPHGTGKQ
                     VRVLVLCTPDKEEAARQAGAEFVGLDEYIEKIKGGWTDVDVIITMPTVMGKIGALGRI
                     LGPRGFMPNPKSGTVTMDVDKAVKEVKQGKIDFKVDKFGIIHASIGKVSFEPEKIVDN
                     AIEFLNTIIKLKPTTAKGTYLKSIYLSTTMSPGLAIDTKSIAAAN"
BASE COUNT          353 a          318 c          285 g          408 t
ORIGIN      
        1 gcagcgggtt caataccata ttcgtcttta agaatcttag ctaactcgtt aacttcctta
       61 accgaaaggt taactaaatc ttctgcaaat ttcttgagat ctgccatttt gataagtgtt
      121 taattgattt caaactttaa gttatttttc cttttcagaa agtgttttaa caacaccaga
      181 tagaatattt cctcctgatt gaagagaagc gataacattc tttgcgggtg attgcagcag
      241 ggcaataaca tcggcaatga gctcgttctt gctcttcagc gatgcaagca tattcagctg
      301 ttcgtcgccc acatatatgg actcctctac aaaggcagct tttagtaagg gtttcggatg
      361 ttcagttcgg aactccttga taagcttagc cggcacattc cccgattcgg taaacattac
      421 cgatgtggtg ccctttaact ctgtaaatag cggcgaaaag tcaaatttgc cgctttgttc
      481 cagggctttc ttaaacaggg tatttttaac caccaccatt ttaacctctt tctgaaagca
      541 cgagcggcga agcttgctag tgtcctcagc attaagcgcc gacgtatcgg tgaggtaaaa
      601 atgcgggtag cggtttatag tttcggtaag cgcatcaatc agattttttt tctcttccct
      661 tgtcatagtg aattgggtct gtaaacggtt ttattaattc gcagcagcta tagatttggt
      721 atcgattgcc aaaccggggc tcatagtagt ggaaaggtaa atgctcttca ggtaggttcc
      781 tttagccgtt gtgggtttga gcttgataat ggtattaaga aattcaatgg cattatcaac
      841 tattttctcg ggctcaaaag aaaccttacc gattgaagcg tgaattatac caaacttgtc
      901 aaccttaaag tcgattttcc cctgcttaac ctccttaaca gccttatcaa cgtccatggt
      961 tacagtgccg cttttagggt tgggcataaa tcctctaggt ccaaggatac ggcccagggc
     1021 gccaatttta cccataaccg taggcatggt aatgataaca tcaacatctg tccaccctcc
     1081 tttgattttt tcaatatact catcaagccc aacaaactcg gctccggcct gacgagcagc
     1141 ttcctcctta tcgggagtac aaagcaccag caccctcacc tgtttgccgg ttccgtgagg
     1201 aagtgtaacc accccgcgaa ccatctggtt ggccttacga ggatcgacat taagcctcac
     1261 gtcgatgtca accgaagcgt cgaactttgt gtaggtgata tccttcaaca gggcagctgc
     1321 ctcagaaagc ttgtactgcc tgttcggctc aaccttcgat aaag
//
LOCUS       DAOOQX010000608         1363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_130921_length_1363_cov_2.918196, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000608 DAOOQX010000000
VERSION     DAOOQX010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130921_length_1363_cov_2.918196"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1363)
                     /locus_tag="PLX53_08855"
     CDS             complement(<1..>1363)
                     /locus_tag="PLX53_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOY73230.1"
                     /translation="HLLEVARICVGEIGLGAQAAIAAILHETLENKQVTRVEIEKHFD
                     SKVAEIVEGYTLISGLDPKDPKTQAELFRQMLITLSGNAVVILIKLADRLETMRSLSR
                     AEPEVRQKRSWETFHLYAPLAHRLGLYPIKSEMEDLAMKHIYPKEYKFIVKKLRETTS
                     SRNRFIRSFIEPIEQELKNRHFDFEIKGRPKSVYSIFRKMQKQDVDFEDVYDVFAIRI
                     ILNTDPEHEKSDCWQVYSIITDKYTPNPERLRDWISAPKSNGYESLHTTVLGPEGKWI
                     EVQIRTARMDEIAEKGFAAHWKYKGIKQEQGVDEWVSMVRNILESPDGSFGDKTDLLQ
                     LNLNSKDIFVFTPKGEIRKLPSKATVLDFAFDIHSEVGSKCTGAIVNGKNAPIKQVLQ
                     NGDIVEVLTSKKQKPKKDWLNFVTTSKAKTRIKQQLREVENQEIARGREMLLRRLKNW
                     KVDN"
BASE COUNT          303 a          341 c          307 g          412 t
ORIGIN      
        1 ggttgtcaac tttccagttt ttaagccggc gaagcagcat ctccctaccc ctggcaattt
       61 cctgattttc aacctcccgg agctgctgtt taattcgggt ttttgctttt gaggtggtta
      121 caaagttgag ccagtctttt ttgggctttt gtttctttga ggtgagcact tcaacaatat
      181 cgccattctg caacacttgc ttgattggag catttttgcc attcacaatg gcaccggtgc
      241 atttcgaccc cacttcggag tggatgtcaa aggcaaagtc cagaacggtg gctttgctgg
      301 gtaattttct tatttccccc ttgggggtaa atacaaatat atccttgctg ttaaggttga
      361 gctgcagcag gtcagtttta tcaccaaaag aaccatcggg cgattcgagt atattcctaa
      421 ccatacttac ccattcgtca accccctgct cctgttttat ccccttatac ttccagtggg
      481 ctgcaaatcc cttttcagct atttcgtcca ttcgtgcggt gcgaatctga acctctatcc
      541 acttgccctc ggggccaagc accgttgtgt gaaggctttc gtagccgttg ctctttggcg
      601 ctgaaatcca atcacgtagc cgttcgggat tgggggtgta cttgtcggtg atgatggagt
      661 aaacctgcca gcagtccgac ttttcatgct cgggatcggt gttaagaatt atcctaatgg
      721 caaaaacatc ataaacatcc tcaaaatcaa catcctgctt ttgcatcttg cggaatatgg
      781 agtacaccga ctttggccta ccctttatct cgaaatcgaa atggcgattt ttgagctcct
      841 gctcaattgg ctcaataaag ctacggataa acctgtttcg gcttgaagtg gtttccctaa
      901 gtttcttaac tatgaacttg tactccttgg ggtagatgtg cttcatagcc aggtcttcca
      961 tctccgattt gatggggtaa agtccaagcc tatgggcaag tggagcatac aggtgaaatg
     1021 tttcccacga gcgcttttgc ctcacctctg gctctgcacg gctgagcgag cgcatggttt
     1081 caaggcggtc ggccagcttg atgagaatta ccacagcatt ccccgatagg gttatcagca
     1141 tttgcctgaa cagctctgcc tgagttttag gatcctttgg gtcaagtccg gatatcagcg
     1201 tgtaaccctc aacaatctcg gctactttcg aatcaaaatg cttctctatt tcaacccttg
     1261 ttacctgctt gttttctagt gtttcgtgaa gtatagcagc aatggcagcc tgggctccaa
     1321 gcccaatttc gccaacacaa atccgggcaa cctcaagcag gtg
//
LOCUS       DAOOQX010000609         1363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_130948_length_1363_cov_2.510703, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000609 DAOOQX010000000
VERSION     DAOOQX010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130948_length_1363_cov_2.510703"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1363)
                     /locus_tag="PLX53_08860"
     CDS             complement(<1..>1363)
                     /locus_tag="PLX53_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOY73231.1"
                     /translation="PVLLNRAPTLHRLGIQAFQPKLIEGKAIQLHPLACTAFNADFDG
                     DQMAVHLPLSNEAILEAQILMLGSHNILNPANGAPITVPSQDMVLGLYYITKPRKGVK
                     GEGLIFYSPEEVIIAYNEHAVDLHAIIKVRLMLPDKNGQLTPQLVETTVGRVLFNQNV
                     PAEMGYINEVLTKKSLRDIIGAVLKKAGTAKAAKFLDDIKDMGYYMAFKGGLSFNLDD
                     VVIPPEKAQLIEEGYAQVDDVMANYNMGLITNNERYNQIIDIWTHTNAKLTLTLLKTL
                     SEDNQGFNSIYMMLDSGARGSKEQIRQLGGMRGLMAKPQKSGSVGNEIIENPILSNFK
                     EGLSVLEYFISTHGARKGLADTALKTADAGYLTRRLVDVSQDVIITENDCGTLRGLVA
                     TAIKKNEDVVETLYERILGRTTVHDVFHPLTGELIVAAGEEITEEIASIIEDSPIEQV
                     EIRS"
BASE COUNT          337 a          329 c          331 g          366 t
ORIGIN      
        1 ccgatcgaat ttcgacctgt tcaattggtg aatcttcaat aatggaggca atttcttcgg
       61 ttatttcctc gcctgcagcc acaataagct caccggttaa cgggtggaat acatcatgaa
      121 cggtagttct tccaagaatt ctctcgtaaa gggtttccac cacatcctca ttcttcttga
      181 tagcagtagc taccaaacca cgtaacgttc cacaatcgtt ctcggtaata ataacatcct
      241 gcgatacgtc aaccaacctt cgggtaaggt aaccggcatc ggctgttttc agagcggtat
      301 cggccaaacc cttacgtgca ccgtgagtag agatgaagta ctccaacacg gatagcccct
      361 ctttaaagtt cgatagaatg gggttctcga tgatttcgtt tcccaccgat cccgactttt
      421 gaggcttggc catcaggcca cgcatacctc caagctggcg tatctgctcc ttcgaacccc
      481 tggctccaga atcaagcatc atgtagatgg agttaaagcc ctggttgtcc tccgaaaggg
      541 tttttagcag ggtaagggta agcttggcgt tagtatgtgt ccagatatca ataatttggt
      601 tgtaccgctc gttattggta atcagaccca tgttgtagtt ggccataacg tcatcaacct
      661 gggcatagcc ctcctcaata agctgggctt tttccggggg tataaccaca tcgtcgaggt
      721 tgaatgaaag tccaccctta aaggccatgt agtatcccat atcctttatg tcgtcgagga
      781 acttggcagc cttagccgtg ccggctttct tcaaaacagc accaattata tcgcgaagcg
      841 acttcttggt tagcacctcg ttgatgtagc ccatttctgc aggaacattc tggttgaata
      901 aaacccgtcc aacagtggtt tcaacaagct gcggggtcag ctgcccattc ttatcgggta
      961 acataagccg aaccttaatg atggcatgta ggtcaacagc atgctcgttg taggcaatta
     1021 ttacctcctc gggcgagtag aaaattaagc cctcgccctt aacgcctttt ctaggtttgg
     1081 tgatgtagta aagaccaaga accatatcct gcgatggaac ggtaataggc gctccgttgg
     1141 cagggttgag aatgttatgt gagccgagca ttaaaatttg tgcttccagg atggcctcgt
     1201 tgctcaaggg caagtggact gccatctggt cgccatcaaa gtcggcgtta aatgctgtgc
     1261 aggcaagggg gtgaagctga attgccttac cttcaatcag cttgggttgg aatgcttgaa
     1321 tacccaagcg gtgaagggtt ggagcacggt taagtagcac ggg
//
LOCUS       DAOOQX010000610         1362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_131173_length_1362_cov_2.009181, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000610 DAOOQX010000000
VERSION     DAOOQX010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131173_length_1362_cov_2.009181"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="PLX53_08865"
     CDS             <1..1037
                     /locus_tag="PLX53_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOY73232.1"
                     /translation="RDGEKLVLRRNAHYFELDNEGKRLPYLESVVVTFVVDKQSEFIE
                     FISGNIDMLSGVHASSKDELLTRTGNLNPKYREKVLMLTGPYLNTEYLGVLLDSIGLE
                     SLENSALHDLRVRKALGYGFDRNAMMLYLRGNLGFPATRGIVPPAMPGFETSTEGFYY
                     NPDKSRQLLAEAGYPNGNGLPPITISTTDDYLDVCEFIQHQLGEVGFDIRIDVLPGAA
                     YRDMMANSKLSLFRASWVADYADPENYLALFVSSNFSPLGPNYTHFSSSEYDQLYRKA
                     LAEPLYDNRLPYYRAMDSLVIANAAVIPLYYDKVVRFISKDVEGITLNPMNLLELKRV
                     RKSYSGLAPS"
     gene            1072..>1362
                     /locus_tag="PLX53_08870"
     CDS             1072..>1362
                     /locus_tag="PLX53_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73233.1"
                     /translation="MIVVADSGATKTDWRIIMEDFSSASFETVGLSPYFNTELDFNDA
                     LTHGFPKGLNPNEVSKVFFYGAGCGTQERGSRVESYLHSFFPAAEIFAWSDAL"
BASE COUNT          345 a          307 c          317 g          393 t
ORIGIN      
        1 ggcgcgatgg tgaaaaattg gtgcttcgtc gtaatgccca ctactttgag cttgacaatg
       61 aagggaaacg actaccttac ctggaatcgg tagtggtaac ttttgtggtg gataaacagt
      121 cagaatttat agagtttatt agcggaaaca ttgatatgct ttccggcgtt catgcctcct
      181 caaaggacga gctgctaacc cgaaccggca accttaatcc gaagtatagg gaaaaggtac
      241 taatgctcac cggcccctat ctgaatacag agtatctggg agtgctcctc gactccattg
      301 ggctggagag cctagaaaat agtgcgctgc acgatttacg ggttagaaaa gctttaggtt
      361 atggctttga caggaatgcc atgatgcttt acctccgggg aaatttagga tttccggcaa
      421 ccagggggat tgtacctcct gctatgcccg gttttgaaac ttcaacagag ggtttttact
      481 acaaccccga taaatcaagg cagctactag ccgaagctgg ctatcccaac ggaaatgggc
      541 tcccaccaat taccatttca acaacagacg attaccttga cgtgtgtgag tttatccagc
      601 accagcttgg cgaggttggc tttgatatta ggattgatgt gctcccgggg gctgcctacc
      661 gtgacatgat ggctaattcc aaactatccc tgtttagagc ctcttgggtt gccgattatg
      721 ccgaccccga aaactacctg gccctttttg tgagtagcaa tttttcaccc cttggaccta
      781 attacaccca tttttcttca tcggaatacg atcaacttta tcgtaaagca ttagctgaac
      841 ccttgtatga taatcgcttg ccatactacc gggcaatgga ttccttagta attgccaatg
      901 ctgctgttat acccctgtac tacgataagg tggtacgatt tatttctaag gatgttgagg
      961 gtatcacctt aaatcctatg aatttgcttg agctaaaacg ggtcaggaag tcatactcgg
     1021 gtcttgcccc tagctgattt tttatggtat taattctaat tcacagataa aatgattgtt
     1081 gttgccgata gcggggccac aaaaaccgac tggcgaatta ttatggaaga tttttcttca
     1141 gcatcgtttg aaaccgttgg ccttagtccc tactttaaca ccgagctgga ttttaacgac
     1201 gccttgacgc atggatttcc taaaggattg aatcctaatg aggtttctaa agtttttttc
     1261 tacggtgccg ggtgcggaac ccaggagcgc ggcagcagag ttgagagcta ccttcactcc
     1321 ttttttcctg cagcagaaat atttgcttgg tccgatgcgc tg
//
LOCUS       DAOOQX010000611         1358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_131817_length_1358_cov_1.759018, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000611 DAOOQX010000000
VERSION     DAOOQX010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131817_length_1358_cov_1.759018"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PLX53_08875"
     CDS             <1..765
                     /locus_tag="PLX53_08875"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOY73234.1"
                     /translation="SISGRIHFITRRYFFGQFYTEIVPQSRYDYYEPRTEGRKLKLPR
                     SIYFNWYGSPDYRKTLALDHQLEYWKADGYNQYGFSYTIAPRIRFSDKLLLVNSWSQS
                     FDYNAIGYFGGSGSNITMGKRDVVTLTNTLSIQFTFSSKSFINLRARHYIRHYKYDSF
                     YTLNLDGTLTPIASPEYSNKNYNLFNVDFYYQWIFAPGSELVFAWKNMEEEMGTIPIN
                     SYFDNVDRVFGSPQYNSFSIKILYYIDYQMIARLAR"
     gene            794..>1358
                     /locus_tag="PLX53_08880"
     CDS             794..>1358
                     /locus_tag="PLX53_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HOY73235.1"
                     /translation="MINKHGFASDNNSGVHPAILQAIMEANQGHTLAYGEDMYTEKAK
                     ALFRELLGTDVEVFFVFNGTAANVLGLQAATQRHNAVICAETAHINVDECGAPERITG
                     CKLLTVKTPDGKLTPSLVSTQMHGFGFQHHSQPKVISISQASELGTVYTPEEVKALSD
                     FAHSHGLYLHMDGTRITNAAVSQSCSLK"
BASE COUNT          378 a          313 c          297 g          370 t
ORIGIN      
        1 tccatttcgg ggaggattca ttttattacc cgtcgatatt tttttggtca attttacacc
       61 gagattgtac cccagtccag gtatgactac tatgagccta ggaccgaggg ccgaaagctc
      121 aagctgccgc gaagcatata ctttaactgg tatggttcgc cagattaccg gaaaactctt
      181 gcactcgacc accagctgga gtactggaaa gccgatggct acaaccaata cggattttcc
      241 tacaccattg ctccccggat tcgctttagc gataagctgc tgctggtgaa ctcatggtcg
      301 caatcgttcg actataatgc cattggatac tttgggggta gcggcagcaa cattacaatg
      361 ggtaaacgag atgtggttac ccttaccaac acgcttagca ttcagtttac cttcagctca
      421 aaatctttta tcaaccttag ggcaaggcac tacatacgcc actacaagta tgatagcttc
      481 tacaccctaa atttggatgg aacgcttaca ccaattgcca gccctgaata ctctaacaaa
      541 aactacaacc ttttcaatgt tgatttttac taccagtgga tatttgctcc cggaagcgag
      601 ttggtttttg catggaaaaa catggaggaa gaaatgggca caattcctat taatagctat
      661 tttgacaatg tggacagggt ttttggaagt ccacagtaca acagcttctc aatcaaaatt
      721 ttatactaca ttgattacca gatgattgct aggttagcca gatagttttt aacaaccttt
      781 taagtttaca tatatgataa acaagcatgg atttgccagc gacaacaatt caggtgttca
      841 tccagccatt ctgcaggcta ttatggaggc aaaccagggg cataccctgg cttatggcga
      901 ggatatgtac accgaaaagg ccaaagcgct ctttagggag ttgcttggaa ccgatgttga
      961 ggtatttttt gttttcaacg gcaccgctgc aaacgtgttg ggtttacaag ctgcaactca
     1021 acgccataat gcagtaattt gcgccgaaac agcccacatt aacgttgatg agtgtggtgc
     1081 acccgagcga attaccgggt gcaagctgct cactgttaaa actcccgatg gaaagctaac
     1141 acctagtttg gtaagcaccc agatgcatgg cttcgggttt caacaccatt ctcagcctaa
     1201 agtaatttct atttctcagg ccagtgagct gggaacggtt tatacacccg aagaagtgaa
     1261 ggcactttcc gattttgccc atagccatgg gctttacctg cacatggatg gaacccggat
     1321 taccaatgct gccgtttcgc aaagctgcag tttaaaag
//
LOCUS       DAOOQX010000612         1357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_131884_length_1357_cov_3.289555, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000612 DAOOQX010000000
VERSION     DAOOQX010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131884_length_1357_cov_3.289555"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLX53_08885"
     CDS             complement(<1..357)
                     /locus_tag="PLX53_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HOY73236.1"
                     /translation="MSSWIIYAIGFIAQALFSSRVFLQWIISEKHKKVLTPKLFWELS
                     LLASVLLFIYGYLRHDFPIMLGQVITYFIYIRNIQLENQWKKFPKWLRVTLFVFPFVI
                     LIYGYNNNIMDRANLFN"
     gene            complement(361..1092)
                     /locus_tag="PLX53_08890"
     CDS             complement(361..1092)
                     /locus_tag="PLX53_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY73237.1"
                     /translation="MDFELTIIVPVYNEEDNLERVYQELSSYIGTAERKAKVLLVNDG
                     STDGSLAKVKEICTHDNRFFYCSFDRNYGLSAAIKAGFDYADTPWVGYIDADLQTSPQ
                     DFNLLFEYANDYDLVTGVRSMRKDSFVKNMSSLIANNIRRAFTHDGMDDTGCPLKIIR
                     TEFAKRIPMFNGLHRFLPAMILLQNGRIKQVPVRHYPRVAGKSKYHLWNRLFGPLMDC
                     FAYLWMKKKYINYHVAENNIQPPTL"
BASE COUNT          355 a          281 c          346 g          375 t
ORIGIN      
        1 attgaacagg ttggctctgt ccatgatgtt gttgttgtag ccgtatatca ggatgacgaa
       61 cggaaaaacg aagagggtaa cacgtagcca tttgggaaat tttttccact gattttccag
      121 ctgaatgttc ctgatgtaga tgaagtaggt gataacctgt cccagcatga tggggaagtc
      181 gtggcgcaag tacccgtaaa tgaaaagcag caccgacgcc agcaagctca actcccagaa
      241 tagctttggg gtgagtacct ttttatgctt ttcggaaata atccattgca ggaaaacccg
      301 acttgagaaa agcgcttgtg ctataaaccc aatggcgtat attatccagc tgctcatggg
      361 ttatagggtg ggaggttgaa tgttgttttc ggccacatgg tagttgatgt acttcttttt
      421 catccatagg taggcaaagc agtccatcag gggtccaaag agcctgttcc agaggtggta
      481 cttcgatttg ccggctacgc gtggatagtg cctcacaggt acctgcttaa tgcgtccgtt
      541 ttgcaggagt atcatggccg ggaggaaacg gtgaaggccg ttaaacatgg gaatacgctt
      601 ggcgaattct gttctgataa ttttaagggg gcagccggta tcgtccattc cgtcgtgcgt
      661 aaaggctctg cgtatattgt tggctatgag cgacgacatg ttcttcacaa aggagtcctt
      721 ccgcatagac ctgacgcctg taaccaggtc gtagtcgttg gcatactcaa acaggaggtt
      781 gaaatcctga ggtgaggttt gcaggtcggc atcaatgtag ccgacccatg gcgtgtcggc
      841 atagtcgaag ccggccttaa tggctgcgct taacccgtaa ttacggtcaa atgaacagta
      901 gaagaagcgg ttgtcgtgcg tgcagatttc cttaaccttg gcaaggctgc cgtcggtgga
      961 gccatcgttt accaacagca ccttagcttt cctttcagct gttccgatgt agctgcttag
     1021 ctcctggtaa actcgttcca ggttgtcctc ctcgttataa accggaacaa tgattgtcag
     1081 ctcaaaatcc atattgttta gataattccg ttgttgcctt aggattcact aaaaatagcc
     1141 agctatttca aagttgtaaa agtataaaag gttttacact tttagtaagc tgttaaaatg
     1201 ttgttcttat taggggaaca aaattcatgc acaagagttt tccctgagaa aaagattatt
     1261 gggcttgaaa gcgagcgggg gaaagtcaaa agttaaatgg ctttatggaa gtttttacac
     1321 cacactaacc cgttggagcc taaaacgttc cgccaaa
//
LOCUS       DAOOQX010000613         1356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_132065_length_1356_cov_2.927748, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000613 DAOOQX010000000
VERSION     DAOOQX010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132065_length_1356_cov_2.927748"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLX53_08895"
     CDS             <1..356
                     /locus_tag="PLX53_08895"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOY73238.1"
                     /translation="SGGTTDQILKIVNECPTNALTFSWLENSRNEHETSPKVVREIKP
                     TSEAGTENVKPVKIQVMPNGPLLLSGDFQLFDADGVELKSMKMVSVCRCGHSQGKPFC
                     DGMHFKIGFRDKEEQ"
     gene            359..574
                     /locus_tag="PLX53_08900"
     CDS             359..574
                     /locus_tag="PLX53_08900"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOY73239.1"
                     /translation="MIESAKHAASLHFLKHGPVRVEGTFRIVDIDGNELEVAEAGKVY
                     LCTCGKSNTKPFCDCSHQEELKLRATN"
     gene            complement(576..>1356)
                     /locus_tag="PLX53_08905"
     CDS             complement(576..>1356)
                     /locus_tag="PLX53_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOY73240.1"
                     /translation="NGVRVTFDCALIVIHGNPGENGLLQGYFEMLNIPYTTGGVLNEA
                     ITFNKHFCKLLLTGTGVKLAREVLVHRGEEIDVDAVAGKLGLPVFVKPNASGSSYGVS
                     KVKHPDEILPALNRAFSEDSTVIIEEFIAGRELTCGAFKSFNREFILPVTEIVPKKEF
                     FDYEAKYLNKSKEITPAPIPEDLYGTIQGLTSKIYDVLECRGIVRVDYIAGANGIYFL
                     EVNTVPGMSEASIVPQQVRVMGENMRDMLTLVIEDALRRAK"
BASE COUNT          379 a          335 c          314 g          328 t
ORIGIN      
        1 tgagcggggg aaccaccgat cagatactta aaattgtaaa tgagtgtccg acaaacgccc
       61 tcaccttcag ctggctcgaa aacagcagaa acgagcatga aacctctccc aaggtggtca
      121 gggagattaa gccaacaagt gaagcaggca ctgagaatgt taaaccggtg aaaattcagg
      181 taatgcccaa tgggccgttg cttctatccg gagattttca gctattcgat gctgatggtg
      241 tcgagctgaa aagcatgaaa atggtatcgg tgtgccgatg tgggcactcg cagggaaagc
      301 cattctgcga cggcatgcat ttcaaaattg gatttaggga taaggaggag caataacaat
      361 gatcgaatcg gcaaaacatg cagcatcgct gcatttctta aagcatggcc cggtgagggt
      421 tgaggggaca ttcaggatag ttgacattga cggaaacgag ctggaggttg ccgaagccgg
      481 taaagtttac ctttgcacct gcggaaaatc gaacaccaag cccttttgcg attgcagcca
      541 tcaggaagaa cttaagctaa gagctactaa ctaaactatt ttgcccttcg cagggcatcc
      601 tcgataacca gcgttagcat atcgcgcata ttttcaccca ttaccctcac ctgctggggc
      661 acgatgctag cttcgctcat acccgggacg gtgtttacct ccagaaagta aatgccattt
      721 gctcccgcaa tatagtcaac cctgacaatt ccccgacatt ccaaaacgtc atatatttta
      781 gaggttagcc cctgtatggt tccatacagg tcttcaggaa taggagcggg agtaatctct
      841 ttggatttgt taagatactt ggcttcatag tcgaaaaact cctttttggg aacaatctcg
      901 gtaacgggca gtatgaactc cctgttaaac gacttaaaag caccacaggt cagctcccta
      961 ccggcaataa actcctcaat aatgacagtg ctatcctcgg aaaatgccct attgagagca
     1021 ggcagaattt cgtccgggtg cttcaccttg ctaaccccgt agctgctacc gctggcgttg
     1081 ggcttcacaa aaacaggcaa cccgagttta ccagctacag catcaacatc aatttcttct
     1141 ccccgatgaa ccaaaacctc tcttgccagc tttacccctg tacctgtgag gagcaacttg
     1201 cagaagtgct tgttgaaggt aatagcctcg ttgagcacac cacccgttgt gtagggtatg
     1261 ttgagcattt caaagtatcc ctgaagtaag ccgttctccc cggggtttcc atgaataaca
     1321 attagtgcac agtcaaaagt taccctaacg ccattc
//
LOCUS       DAOOQX010000614         1356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_132074_length_1356_cov_2.757879, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000614 DAOOQX010000000
VERSION     DAOOQX010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132074_length_1356_cov_2.757879"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1356
                     /locus_tag="PLX53_08910"
     CDS             <1..>1356
                     /locus_tag="PLX53_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOY73241.1"
                     /translation="MPFPRMYSSDPQHISAYKSWTNFKGRPVNVNTQDGKQQTIYTPT
                     FGENLKFFFSYQLGFMYFRYFMWNFSGRQNDIQGHGDVMKGNWICGIPFIDNARLGSQ
                     ELLPDTYKQNKGRNRYFMLPLILGLSGLIFQYRRRMKDFWVVMTLFVMTGIAIVVYLN
                     QTPYQPRERDYAYAGSFYAFSIWVGLGVMALYELTRRIKDGSATALATGAACTLMAPT
                     LMAFQNWDDHDRSGSYIPADFGYNMLVGCKPNSILFTYGDNDTFPLWYNQEVEGVRTD
                     VRVANLSYLRGDWYIEQMQRKVYESDPLPLRMTRKQYFSGKRDVVLVFDRIKEPINLN
                     MAVNFMLSDDPAAELKSPFDPGERINYIPSSNLFLPVDRQQVIKTQTVLPSYYDRIVD
                     TMRWKLNKNMVMKDGQVILDLLGTNSWVRPIYFGTTVNSDTYHGLNKYFHLEGLMYRI
                     LP"
BASE COUNT          345 a          315 c          345 g          351 t
ORIGIN      
        1 atgcctttcc cgcgcatgta cagcagcgat ccgcagcaca tcagcgccta taaaagttgg
       61 accaacttta agggtaggcc ggttaatgtt aacacccagg atggtaagca gcaaaccatc
      121 tatactccta cttttggcga gaatctgaaa tttttcttca gctaccagct gggtttcatg
      181 tacttccggt acttcatgtg gaacttttcc ggacggcaaa atgacattca gggccatggc
      241 gacgtgatga agggcaactg gatttgcggg attcctttta ttgataacgc cagacttggt
      301 tcgcaggagt tactccccga cacctacaag cagaacaagg gacggaacag gtatttcatg
      361 cttccgctca ttctcggatt atcagggttg atattccagt acaggcgtag gatgaaggat
      421 ttctgggtgg tgatgacgtt gtttgtaatg accggcatag ctattgtggt ttacctaaac
      481 caaaccccat accagcctcg agaaagagat tatgcctatg ctggtagctt ttacgctttc
      541 agcatttggg ttgggttagg tgtgatggcc ctatacgagc ttactcgtag aattaaggat
      601 ggttcagcca ccgctttagc cacgggcgct gcttgcaccc tgatggcgcc taccctgatg
      661 gccttccaga actgggacga tcacgaccga tcgggaagct atattcctgc cgattttgga
      721 tacaacatgc tggttggctg caagccaaac tccatcctgt ttacctatgg cgataacgat
      781 acctttccct tgtggtataa ccaggaggtg gagggcgtac gaaccgatgt gagggttgcc
      841 aacctcagct accttcgggg tgactggtat attgagcaga tgcagcgcaa agtttatgag
      901 agcgatcccc ttccgctgag aatgaccagg aaacagtact tctccgggaa acgagatgtg
      961 gtgctggttt tcgacagaat taaggagcca atcaacctga acatggcggt aaacttcatg
     1021 cttagcgacg atcccgctgc tgagctgaaa tcgccattcg atccgggcga gaggattaac
     1081 tatattccct ccagcaacct gtttttgccg gttgacaggc agcaggttat aaaaacccaa
     1141 accgttttgc cctcatacta cgacagaatt gttgatacca tgcggtggaa gctgaataaa
     1201 aatatggtga tgaaggacgg gcaggtaatt ctcgacctgc tgggaactaa tagctgggtg
     1261 cgcccaattt actttggaac tacggtgaat agcgatacat accacgggct taataagtat
     1321 ttccaccttg aaggattgat gtacaggatt ttacct
//
LOCUS       DAOOQX010000615         1353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_132497_length_1353_cov_3.185670, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000615 DAOOQX010000000
VERSION     DAOOQX010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132497_length_1353_cov_3.185670"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..>1353)
                     /locus_tag="PLX53_08915"
     CDS             complement(147..>1353)
                     /locus_tag="PLX53_08915"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOY73242.1"
                     /translation="NPRFGSQPPAKNATIADLKTACTSNLVKITQDFVVEAVVLANDK
                     SGNIYKSLFIEDESGAIEFKIDVYDLFVDFPVGTKIAISCKDMYVGKYGGVVQLGGVY
                     NNAIGRISANEFYQKVFVVGSGFEVTPIPVSINELTADLVGKLVTISDVQYIDSELGK
                     TYAESSITNRHVEDALGSTAIVRTSDRADFAGITLPGKRGSITAVLSKYYNDYQLYIR
                     DLTDVWMTQPRRVKNYILKQDFSSATANSPISVGGWQSIAEVGTRRWMAKVYSGNTYA
                     EMNPYQSGEASNIGWLISPAIPVASGVTTYLTFNTQFAYWANSTLEVFVSENYDGANP
                     ASATWTKLSNAHIVTQSEGQNTWVSSGLVDISEFPGTIYLGFKYSGGGSIGQTTAFRV
                     DDVNVFTQQ"
BASE COUNT          357 a          322 c          314 g          360 t
ORIGIN      
        1 gttccgcaag taggcggaca agttacgccg ggaggcggac aagtcttgaa accactttat
       61 actttatctt agaatttgtg ggttaaagta aaggccaacc gataatccgg ttggccttcg
      121 agtaccaaag taacagctaa ataattttac tgctgtgtaa aaacattaac gtcatcaacc
      181 ctaaaggcgg tggtttggcc aatagaacct cctccagagt acttgaaccc gagataaata
      241 gttccgggaa actccgaaat atcaaccagg ccggagctta cccatgtgtt ttgcccttcg
      301 ctctgcgtta cgatgtgagc attggaaagt tttgtccatg ttgccgaggc agggttagct
      361 ccatcgtaat tttccgacac aaatacctcg agcgtggagt tggcccagta ggcaaattgg
      421 gtgttgaagg taagataggt tgttacgccc gaagcaacag gaatagcagg ggaaatcagc
      481 catccgatat tactggcttc gcccgactgg tatggattca tttcggcata ggtgttgccc
      541 gagtaaacct tggccatcca tctgcgtgtt ccaacctccg caatggattg ccatccccca
      601 acgctaattg gactgtttgc ggttgcagag ctaaagtcct gtttaagaat gtagttttta
      661 acccgacgtg gttgggtcat ccaaacgtcg gtaaggtcac gaatgtaaag ctggtagtcg
      721 ttgtagtact tgctcagcac ggcggttata cttccccttt tgcctggaag ggtaataccc
      781 gcaaaatcag cacggtcact ggttctcaca atagcggtac tacccaaagc gtcctccaca
      841 tgacgattgg tgatactcga ctcggcgtat gttttgccca actcggaatc tatatactga
      901 acatcagata tggtaactaa tttgcctacc aggtcggctg ttagctcgtt gatgctcaca
      961 ggaattgggg ttacttcaaa tcctgaacca accacaaaga ctttctggta gaactcgttg
     1021 gcggaaatcc tgccaatggc attgttgtaa acgccaccca gctgcaccac accgccatat
     1081 ttacccacgt acatatcctt acaggaaatg gcaatttttg ttccaaccgg aaaatcaaca
     1141 aataggtcgt acacgtctat cttaaactct attgcccccg attcgtcctc aataaacaga
     1201 cttttgtaaa tattcccgga tttgtcgttg gctagtacaa ccgcctcaac cacaaaatcc
     1261 tgtgtaattt tcaccaggtt tgaggtgcaa gcggtcttta aatcagcaat ggtggcattt
     1321 ttagcaggtg gttgagagcc aaagcggggg ttg
//
LOCUS       DAOOQX010000616         1353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_132523_length_1353_cov_2.720339, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000616 DAOOQX010000000
VERSION     DAOOQX010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132523_length_1353_cov_2.720339"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..865)
                     /locus_tag="PLX53_08920"
     CDS             complement(17..865)
                     /locus_tag="PLX53_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73243.1"
                     /translation="MKSKANILALLLAMMATTAIAQDSFIVTRIKGNVVNYRTGTSLR
                     AGDVLQPSDKVTFDNFDSYVISIGQNMGRYMIKLHEPPAPDAIQQLTAIVKDVAVPTK
                     RRSLMAERYRPNESVVEDLGSYFGNDKFSVIGNEVLVPVNPDKFQLNDSRFIVFYYRV
                     NNNPVSKKIGYEQNVLKIEKDKLLNTSAGTISNDEIPNVAVYRYESNTHSSEEITRFN
                     LIFVDKEQLTNEFLTILPILHRQNMSKDDIKKYLIEYYYDFYGVTDSKSIKKFVDDLV
                     DGSAGN"
     gene            complement(984..1307)
                     /locus_tag="PLX53_08925"
     CDS             complement(984..1307)
                     /locus_tag="PLX53_08925"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOY73244.1"
                     /translation="MNVEEIRDYCLSKPGATESLPFDDETLVLKVGGRIFAMISLSGD
                     SSLTLKCDPEIALELQEKYTFVEPGYHMNKKFWITLRDTTLIPRNLVMQWIDSSFVLV
                     AAGKK"
BASE COUNT          353 a          288 c          299 g          413 t
ORIGIN      
        1 gtggtagtga cctaaactaa tttccagcgg agccatcaac caaatcatct acaaattttt
       61 taattgattt ggaatcggtt acaccgtaaa agtcgtagta gtactcaatg agatactttt
      121 taatgtcatc cttgctcata ttctgccgat gaaggatggg cagaattgta agaaactcgt
      181 tggtaagctg ctccttatca acaaaaatca ggttaaatct ggtaatttcc tcgctggaat
      241 gggtgttact ttcgtacctg taaacggcaa catttggaat ttcgtcattg gaaatagttc
      301 ctgcgctggt gttgagcagc ttatcctttt caattttaag cacattctgt tcgtatccga
      361 ttttcttgga aaccggatta ttgttgaccc tgtagtagaa aacaatgaac ctgctatcgt
      421 tcagctggaa cttgtcagga tttacgggga cgagcacctc gttgccgatg accgaaaatt
      481 tatcgttgcc gaagtagctt cccaagtctt caaccacact ttcgttgggc ctataccgtt
      541 cagccataag gcttctgcgc ttagtgggta cagcaacatc ctttacaatg gcagtaagct
      601 gttgaatggc atcaggggca ggaggctcat gaagcttaat catgtagcgg cccatattct
      661 ggccaatgga gataacgtag gagtcaaagt tatcaaaggt aaccttatcg ctaggttgga
      721 gtacgtctcc cgctctgaga gatgttcccg ttcggtagtt aaccacattc cccttaattc
      781 gggttacaat aaaactatcc tgagcaattg ccgtggtagc catcatagcc agaagaagag
      841 ctagaatatt tgctttagat ttcatggcgt taggtttttg ttattgcttt tttttgattt
      901 catcaattcc tgtaccaaaa tagcgaaatg ttgggatttg ccatcccttt aacttgctcc
      961 gacaggtgat gcaaagtatt gccctatttt ttgcctgcag caaccagcac aaaggagcta
     1021 tcaatccatt gcatgacaag gtttctcggt ataagggttg tatccctaag ggttatccag
     1081 aattttttgt tcatgtggta gccgggttca acaaaggtat atttttcctg cagctcaagg
     1141 gcaatttcgg gatcacactt caaggttagg ctgctatcgc ccgaaaggct tatcatggcg
     1201 aatattctgc cacccacctt tagcaccaag gtttcatcgt cgaacgggag gctctcggtt
     1261 gcgccaggtt ttgaaaggca gtagtcacga atttcttcaa cattcatacc gttaaagtgt
     1321 tgggtaaaag gcatcctcga tatgctctat agc
//
LOCUS       DAOOQX010000617         1352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_132643_length_1352_cov_3.369314, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000617 DAOOQX010000000
VERSION     DAOOQX010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132643_length_1352_cov_3.369314"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PLX53_08930"
     CDS             complement(<1..693)
                     /locus_tag="PLX53_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY73245.1"
                     /translation="MDESKVIEISNANIFQEDNLILSDVNLSLAQGEYLYIIGRVGSG
                     KTSLIKTITAELPLKSGEATVCGFNLRTIKRRHIPKLRRKIGVVFQDFQLLSDRSVYD
                     NLLFVLRSTGWKDKQAIKQRITEVLEMVGLKFKDYKLPHQLSGGEQQRVVIARALLNN
                     PEIILADEPSGNLDMESSEGIMQILHSIAESGKSVVMATHNHQLIKKFPARTLKCEKG
                     KLLEIEQMGEIDF"
     gene            929..>1352
                     /locus_tag="PLX53_08935"
     CDS             929..>1352
                     /locus_tag="PLX53_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73246.1"
                     /translation="MSTSDKNDILSVLYEAIPAGNKQGEDIFTFGSVYVAASTSEGNI
                     GVCATLGVTVPCFNPAYFNIDRVADRVALNALINARVNYSVLYDGAADIFDVVDFTRY
                     QSIVMIGYFRSLVDKLSSSGVETFIFDLNQHDVPVMPIK"
BASE COUNT          337 a          289 c          325 g          401 t
ORIGIN      
        1 gaagtcaatt tctcccatct gctcgatttc gaggagtttt cccttttcac acttcaaagt
       61 gcgggcagga aactttttaa tcagctggtg gttatgggtg gccattacaa ctgactttcc
      121 actttcggca atgctatgga gtatctgcat aatcccttcg gacgattcca tgtccaggtt
      181 acccgagggc tcgtcggcca gaattatttc ggggttgttg agaagcgcac gggcaatgac
      241 tacccgctgc tgctcacccc cagaaagctg atggggcaac ttgtagtcct taaactttag
      301 cccaaccatt tcaagcacct cggttattcg ctgcttgatg gcctgcttat ccttccagcc
      361 ggttgacctt agcacaaaca gaaggttgtc ataaacgctc cgatcagata gcagctgaaa
      421 atcctgaaac acaaccccaa ttttacggcg aagcttgggg atatgcctgc gtttgattgt
      481 tcgaaggttg aatccacaaa ctgttgcctc gcccgattta agggggagtt cagccgttat
      541 ggtttttatt aggctggttt ttccgctgcc aaccctaccg atgatgtaga gatattcgcc
      601 ctgagccagt gacaggttaa catcggaaag tatgaggtta tcctcctgga agatgttggc
      661 attgctaatt tctattacct tgctttcgtc catggtatgg gttaagcgca aaatttgttg
      721 ctaaggtata atatttattt aaacgctagg ataccacatc taagggttgg ctttgtattt
      781 tttactgttt agatgaaagg agtgccatgg ataaagttgt aggtagtact taactacccc
      841 tctacctttg gcagaatggg ggatgtgaat ttttcagcta cttttggcat ttggtacaaa
      901 cagttatttc ggtaaataaa aatatggcat gagcacgagt gacaaaaatg atatcctgtc
      961 tgttctttat gaggctatac ctgctggcaa caagcaggga gaagacattt ttacctttgg
     1021 atcggtttat gtggcggctt caacaagcga ggggaacatt ggtgtatgcg ccacgttggg
     1081 tgttaccgta ccctgcttta accctgccta ttttaatatt gaccgggtag ccgaccgggt
     1141 agcgctgaac gcactgatta acgccagggt aaactattcg gttttatatg atggcgcagc
     1201 agatatcttc gatgttgtag attttacaag gtaccaaagc attgtgatga ttgggtattt
     1261 caggtcgctg gttgacaagc tcagcagcag cggggtcgaa acatttattt tcgacctgaa
     1321 ccaacacgat gtacctgtaa tgccaataaa gc
//
LOCUS       DAOOQX010000618         1341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_134478_length_1341_cov_3.474339, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000618 DAOOQX010000000
VERSION     DAOOQX010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134478_length_1341_cov_3.474339"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLX53_08940"
     CDS             <1..234
                     /locus_tag="PLX53_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY73247.1"
                     /translation="FPNPADKKLNIRIDGENEATFTIDLLNHQGIRVNSAKLQVPSEL
                     STTFDVEGLAPGIYFLHVYSSKNSTVFKVVVKR"
     gene            590..>1341
                     /locus_tag="PLX53_08945"
     CDS             590..>1341
                     /locus_tag="PLX53_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY73248.1"
                     /translation="MYKIQTHPILEVPKTESITFLFNGNEVKGAKGFTIAAALHQAGY
                     PVHSHSLENRERSLECGIGKCGACEMLVDGQIKRICITPVDGVKEVSEIPKDYAPGVK
                     PADKLHSTRVYKTTVAIIGAGPAGLAARELLLQHGIENIVIDNNATIGGQFNMQTHQF
                     FFFEKEKKFGGMRGFEIAKTLAGDNAEGIFLNSTVWDILENKRIAVKNIRTDEIYFVE
                     ADHLVVATGAVPFMPTFENDDLPGVYTAAVVQ"
BASE COUNT          402 a          296 c          297 g          346 t
ORIGIN      
        1 tttccaaacc cagcagacaa aaaactaaac attagaattg acggggagaa tgaagctacc
       61 tttaccattg acctcctaaa tcatcagggc atcagggtca actcggccaa attgcaagta
      121 ccttcggagc ttagcactac cttcgacgtt gagggattgg ctccggggat ttacttcctg
      181 catgtttact catctaagaa ttccactgtg tttaaggttg ttgtgaaaag ataaccaaca
      241 aattcggtag gcaaggtgct aaacgctcaa cccagaattt cactgaaatg gggttgggcg
      301 tttggcagta ttatctggtc aataaacagg tctactgaaa atgggcattc ccaaaagcga
      361 gcctacaacc ctttctttcc atcaccttct acattgaaat aaaacatgca accacttaat
      421 tttaagattg aggtcaatga gtaaacaacc cctctcataa gttttacccc acgaattcac
      481 atatttttta taaaccctga cgaatatcat taacagccat acttggtaat tcatactttt
      541 gtaatcaaat tacaagaaat tctatcatag ttcttctaaa tggcaagtta tgtataaaat
      601 tcaaacacac cccatacttg aggttccaaa aacggaaagc attacttttc tatttaacgg
      661 taatgaagta aagggggcaa aaggatttac cattgcagcg gcgcttcatc aggcaggata
      721 tccggttcat agccacagtc tcgaaaatcg ggaacgtagc ctcgaatgcg gtataggcaa
      781 gtgtggagca tgcgagatgc tggtagatgg tcaaattaaa cgtatttgca ttacccctgt
      841 tgatggggtt aaggaggtta gtgaaatacc caaggactat gcaccgggcg taaaacctgc
      901 tgataagctg cacagcacca gggtttataa aaccaccgtg gccatcattg gcgccgggcc
      961 tgccggcttg gcggcacgcg agctgctgct tcaacatggt attgagaaca ttgtgattga
     1021 taacaacgca acaattggcg gacagttcaa catgcaaacc caccagttct tcttctttga
     1081 aaaggagaag aagtttggcg gaatgcgagg cttcgagatt gccaaaaccc tggccggcga
     1141 taacgccgag ggtatttttc tgaacagcac cgtatgggac attctcgaga acaagcgcat
     1201 tgcggttaaa aatatccgaa cggatgaaat ctactttgtt gaggccgacc acctggttgt
     1261 agccacagga gcagttccgt ttatgcccac ttttgagaat gacgatttac ccggtgttta
     1321 cacggcagcg gttgttcaga a
//
LOCUS       DAOOQX010000619         1340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_134647_length_1340_cov_3.120623, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000619 DAOOQX010000000
VERSION     DAOOQX010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134647_length_1340_cov_3.120623"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLX53_08950"
     CDS             complement(<1..712)
                     /locus_tag="PLX53_08950"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOY73249.1"
                     /translation="MLSKIKNYLAKLHIPYFPFARYLKAGGSAIGFMLIGISGGHSQI
                     VSQPVTRHITVDEGLAASEVYHVFQDSRGYIWFGTNNGVSRFNGYEFENFDLVSGLPD
                     NTVFEIYEDYKHRIWFIPFSGSLSYYENGEIKSYRYNSKIVEHLPNSRGPSKLSFFVD
                     SLDNVSFGLKDFGIISIDQEGIYKHIDDSNTDAQVVVRSVGSGKFLVSDSHEPRKYGL
                     FYFDGRQSYRFTLSELGIS"
     gene            complement(971..>1340)
                     /locus_tag="PLX53_08955"
     CDS             complement(971..>1340)
                     /locus_tag="PLX53_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOY73250.1"
                     /translation="IKEAEAYPGPSLIIAYSPCINHGIRKGMGSTQSEAKQAVKSGYW
                     HLYRYNPLLEAQGQNPFQLDSKEPDWGMFQDFLKGEVRYTSLQLSFPDQANELFKAAE
                     ENAKWRYNSYKKLAGVVEKP"
BASE COUNT          394 a          304 c          265 g          377 t
ORIGIN      
        1 ggctaatgcc cagctcgctt aaagtaaacc tgtatgactg ccttccatca aagtagaata
       61 ggccatactt ccgaggttca tgtgaatcgg aaactaaaaa ttttcccgac cctacgcttc
      121 tgacaacaac ttgggcatcg gtgttgctat cgtcgatatg cttgtaaatc ccctcctggt
      181 ctattgaaat gattccgaag tcctttagac caaaagaaac attgtccaag ctgtccacaa
      241 aaaatgacag cttagaaggg cccctgctgt tgggaagatg ctcaacaatt ttggagttgt
      301 acctatagct ctttatttct ccgttttcat agtatgagag ggaacctgag aacggtataa
      361 accatattct gtgcttataa tcctcgtaga tttcaaaaac cgtattatca ggcaaccccg
      421 ataccaagtc gaagttctcg aactcatagc cgttaaaacg gctaacgccg ttgttagtcc
      481 caaaccagat atatcctcgg gaatcctgaa aaacatggta aacttcgctt gcggcaaggc
      541 cttcatcaac tgtaatatga cgtgttacag gctgggacac gatttggcta tgcccaccgc
      601 ttatgcctat caacataaac cctatggcac ttcctcctgc tttaagatag cgggcaaaag
      661 gaaagtaggg aatatgcaac tttgctaaat aattttttat cttagacaac atgccacagg
      721 atttttattg agtaacttta taatcatccc cggttgatat ctttttgcga atcgcaaaaa
      781 attcctatcg gcttataaaa gtaaaaaaac attgcatgaa aaaaaatttg cccatctaaa
      841 tatggcaaat ttcataatca cttttgcaag ttattttaaa cgtaaaaagg cttccctgca
      901 atcaggaaag ccttttacaa atggtctata agaaaatgtg atcagctaaa ataccttcta
      961 cacaatcaaa ttaaggcttc tcaacaacgc cagcaagttt tttataggag ttgtacctcc
     1021 acttagcatt ctcctcggca gctttaaaca gctcgttggc ttgatcgggg aaggaaagtt
     1081 gaagggaggt gtacctcact tcgcccttca ggaaatcctg gaacataccc caatcgggct
     1141 cttttgaatc gagctggaat gggttttgtc cctgagcctc aagtaggggg ttgtagcggt
     1201 acaggtgcca gtaacccgac ttaacagcct gtttcgcctc actttgagtt gaacccattc
     1261 ccttgcgaat accgtggttt atacacggcg aataggcaat tataagcgat ggtccgggat
     1321 atgcctcggc ttccttaata
//
LOCUS       DAOOQX010000620         1339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_134853_length_1339_cov_2.517134, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000620 DAOOQX010000000
VERSION     DAOOQX010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134853_length_1339_cov_2.517134"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLX53_08960"
     CDS             <1..234
                     /locus_tag="PLX53_08960"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HOY73251.1"
                     /translation="QAPVIMMSQNRQESKDRERAKKDYMINLKAELEIRSLHEKIDHL
                     ILRQQQEIVEMQKVQTDMLSDIAKEVVSSSERE"
     gene            complement(249..>1339)
                     /gene="priA"
                     /locus_tag="PLX53_08965"
     CDS             complement(249..>1339)
                     /gene="priA"
                     /locus_tag="PLX53_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOY73252.1"
                     /translation="YGLVELADRYLNMELPQVEVVDVREARKRKLMRSHFSEKLIEEI
                     GLSLERNEQVILFQNRRGFAPFIECHECGWVPHCENCDVTLTYHKASDLLVCHYCGYS
                     VKPTHECMACGSTNLQPQGFGTEKVEDELQIFFPEASIERIDLDSTRSRTAYEGIIAR
                     FETGQTDILIGTQMVTKGLDFDRVSLVGVLNADNMLNFPDFRASERSFQLMAQVSGRA
                     GRKNRRGLVIIQTSQPNHPVIKQVKENDYMAFYATVVGERQKFLYPPFCRLISISIKH
                     KDQNVARQASEVMANGLKHSLGNRVLGPEAPMVGKIQGYYIQSILVKAGGQQSLSRVK
                     ELIAYMVNSLRVNGEYRSLVVAVDVDPQ"
BASE COUNT          348 a          351 c          316 g          324 t
ORIGIN      
        1 caggcacctg ttattatgat gagccaaaac cgtcaggagt ctaaagatcg ggaaagggct
       61 aaaaaggact acatgataaa ccttaaggct gagctggaaa tcaggtcgct tcacgaaaaa
      121 attgatcacc tgattttacg ccagcagcaa gaaatagttg agatgcagaa ggttcaaact
      181 gatatgctca gcgacattgc caaagaggtg gtgagtagct cggaaaggga gtaaagagat
      241 agccctccct actggggatc aacatcaacc gcaacaacca gcgaacggta ctcgccattt
      301 accctcaggc tgttgaccat gtaggcaata agctccttaa ccctcgaaag ggattgctgc
      361 ccgccagcct taaccagaat gctttgaatg taataaccct gaattttccc aaccatcggg
      421 gcttctggcc caagcaccct gttacccaag ctatgcttca acccattggc cattacttcg
      481 gaagcctgtc gggcaacatt ttggtcctta tgtttaatcg aaatggaaat taaccggcaa
      541 aatggtgggt agaggaactt ctgtcgctcc ccaactacag tggcgtaaaa agccatgtag
      601 tcgttttcct tcacctgctt aatcaccgga tggttaggtt gcgatgtttg aatgattacc
      661 aagccccgtc ggttcttacg cccggcacgt ccgcttacct gcgccatcag ctgaaaactc
      721 ctttcggatg ccctgaaatc gggaaaatta agcatgttgt cggcatttag tacacccacc
      781 aggctaaccc tgtcgaaatc caagcccttt gtaaccattt gtgttccgat gagtatatcg
      841 gtttgccccg tctcaaaccg ggcaataatt ccctcgtagg cagttcgtga acgggttgag
      901 tcaagatcta tgcgttcaat tgaagcttcg gggaaaaata tttgcagctc atcctccacc
      961 ttctcggttc caaatccctg cggttgcagg ttggtgctac cacatgccat gcactcatgg
     1021 gtaggtttaa cagagtagcc gcagtagtgg cacaccagca ggtccgatgc cttatggtag
     1081 gttagggtta cgtcgcagtt ctcgcagtgg ggaacccagc cgcactcatg acactcgata
     1141 aatggcgcaa agcctcgcct gttctgaaac agaatgacct gctcattgcg ttcaagggat
     1201 agcccgattt cctcaataag cttttcggag aagtgcgagc gcatcagctt gcgctttcgg
     1261 gcctcccgta catcaaccac ctccacttgg ggcagctcca tgtttaggta gcggtcagca
     1321 agctccacca aaccatact
//
LOCUS       DAOOQX010000621         1337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_135131_length_1337_cov_3.776911, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000621 DAOOQX010000000
VERSION     DAOOQX010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135131_length_1337_cov_3.776911"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1337)
                     /locus_tag="PLX53_08970"
     CDS             complement(<1..>1337)
                     /locus_tag="PLX53_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOY73253.1"
                     /translation="QKGEGLLAVARRYNIDAKTIETYNQEVLKDGLKLGEYLRIPAVK
                     QHPADSLMVTSKPEPVAFFSTCKNNFRYNGQPFDISLLLPFSQGSAVDSDELIDEAEE
                     VKMDPGTTNTLESSRITSQETLTSLNFYEGFLLAVDSMKKAGVSMNINVFDTKHQPEE
                     VTSILNKGKLDKSNIIIGPLFVNEIKPVVAYAERKSIPMVSPLYSGNEVISEYPGLIS
                     VNQGYGEQLEVFIKNFKFSDTCHYVVIYDMEKILTGPIQNFSSALNQRIRESGVRVDS
                     VVHQTAAMKSEEVQAKLHSYMKADGCNVVIIPSQDEPFVSDLLGQLYAVKSLNGLKMI
                     VYGPARWQKMKNVPPDYLYKLNARVFTPFYVDYSRDEVKRFVASFRDRYRAEPNQFSF
                     LGYDVGIYFISALKTYGPDFTKCLKDFQKDMLQSTFTFDVVSNESRLSNTHQF"
BASE COUNT          369 a          288 c          294 g          386 t
ORIGIN      
        1 taaattggtg ggtgttgcta agtctggatt cattcgaaac cacatcgaaa gtaaaggtgg
       61 actgaagcat gtccttttgg aaatctttta aacatttagt aaagtcgggg ccataggttt
      121 tcagcgccga aataaagtaa atgcccacat cataacccaa gaatgaaaac tggttgggtt
      181 cagctctgta cctatccctg aagctggcaa caaatcgttt aacctcgtct cttgaatagt
      241 caacgtaaaa tggagtaaac acacgggcat tcagtttgta caggtaatca ggtggaacat
      301 ttttcatctt ttgccagcgt gcagggccat aaacaatcat cttaagccca ttcaaagact
      361 taaccgcata gagttgcccc agcaaatcgg aaacaaaagg ctcatcctgc gaggggatta
      421 taactacatt acacccgtcg gctttcatgt agctatgaag cttagcttga acctcttccg
      481 atttcatcgc tgctgtttga tgaacaacag agtcaaccct aacacccgat tcgcggattc
      541 tttggttgag tgcagagcta aaattttgaa ttggacctgt aagaattttc tccatgtcgt
      601 aaataacaac atagtggcag gtatcgctaa acttaaagtt ttttataaaa acctccagct
      661 gttcgccata tccttggttc accgatatta gaccagggta ttctgatatg acttcattcc
      721 cactgtagag aggcgaaacc atgggaatcg attttctttc ggcataagcc acaactggtt
      781 tgatctcgtt aacaaacagt ggacctatga taatgttgga cttatcgagt ttgcccttat
      841 ttagaatact tgtaacctct tcgggttggt gtttagtgtc gaaaacgttg atattcatgg
      901 aaactccagc tttcttcatc gagtcaacgg caagtagaaa gccctcataa aagtttaagg
      961 aggtaagagt ttcctgcgag gtaattcttg acgattcaag ggtgttggta gtaccaggat
     1021 ccattttgac ctcttcggcc tcatcgataa gctcgtcgct atcgacggcg ctaccctggc
     1081 taaagggaag aagtagcgaa atgtcaaagg gttggccgtt gtacctaaag ttgtttttgc
     1141 aggtagaaaa gaatgccacc ggttctggtt tgcttgtaac cataagcgaa tcggcagggt
     1201 gctgctttac agcaggtatt ctaaggtact cgcctagctt aaggccgtcc tttagaactt
     1261 cctgattgta agtctcaatg gttttagcat cgatgttata tcgtcgtgca acggccagca
     1321 acccctctcc cttctgc
//
LOCUS       DAOOQX010000622         1336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_135354_length_1336_cov_2.609680, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000622 DAOOQX010000000
VERSION     DAOOQX010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135354_length_1336_cov_2.609680"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1336)
                     /locus_tag="PLX53_08975"
     CDS             complement(<1..>1336)
                     /locus_tag="PLX53_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73254.1"
                     /translation="ISDTIEIVATSEQLPDINAFATITDTLNEGEQRLAKLNGQAKIT
                     ENTNYTIGITSSTNQDSITANNSDNIVFSVSQSVYATDHGATGGFTLGENQPFGNIFK
                     VNKPSFIKGIQVGWSPISTIPEAEYPIPFWVAVYQLDPLDSSCYFPVKTGYFEKQYSQ
                     VGASSDYPFDEPIYCLPGFSYFVTVTQLSSASLGVGCDGNSYGSFWKRNGSTFKPEKL
                     ANPTIGNAAIRLYFNKPIENPIVTFTVKNENNEPVQNARVYVREVDSTLITGVSGEAS
                     IELSNGTYTCRLDSAGFAPVTKQVKVFYQDLFIDFKLQKTYQAKFKVLDEDLLTPIAG
                     AEINILSESIDITFPEEPYLTDQNGLDSLQIAPGYYNFSVRASGYNMSSPGYFFIEPS
                     DTLIEVFMQPATTHSLTLQVIDEKGTSLPGAQVTTLNHGAKLTNDNGKALFSN"
BASE COUNT          334 a          263 c          342 g          397 t
ORIGIN      
        1 cgtttgaaaa taatgcttta ccattatcgt tagttagttt agccccgtgg ttcagtgttg
       61 taacctgggc accgggtaga gaggttccct tttcatcaat aacctgcagg gttaggctgt
      121 gggtggttgc gggttgcatg aaaacttcaa tcagggtatc gctgggttcg ataaaaaagt
      181 agccggggct gctcatgtta tatccggaag ccctgacgga aaagttgtag tatccagggg
      241 caatttggag gctatccaat ccgttttggt cggttaggta gggctcttca gggaaggtaa
      301 tatcaattga ttcagacagt atgttgattt ctgctcctgc aattggagtt agcaggtctt
      361 cgtccagcac cttgaattta gcctggtagg ttttttggag tttaaaatca atgaataggt
      421 cctggtaaaa gacctttacc tgcttggtaa ctggcgcgaa gccagcggag tcgagcctgc
      481 aggtgtaggt accgttggaa agttcaattg atgcttcgcc tgaaaccccc gttataaggg
      541 ttgaatcaac ctcacgaaca tacaccctgg cgttttgcac gggctcattg ttttcattct
      601 ttactgtgaa agtaacaatg ggattttcaa taggcttgtt gaagtaaagc ctgatggctg
      661 cattgccaat ggttgggttt gccagttttt caggtttaaa cgtgcttcca tttcttttcc
      721 aaaacgagcc gtagctgttg ccatcgcacc ccacaccaag actagcgcta cttaactggg
      781 ttacagtaac aaagtaggaa aatcctggta agcagtagat tggttcgtca aaggggtagt
      841 cgcttgaagc gcccacctgg gagtactgtt tttcgaaata tcctgttttt acagggaaat
      901 aacaggaact atccaggggg tcaagctggt aaactgctac ccagaatgga atggggtatt
      961 ctgcttcggg aatagtgctg ataggcgacc agcccacctg aatacccttg ataaatgacg
     1021 gcttgttaac cttgaaaatg ttgccaaagg gttgattctc tccgagtgta aaaccacctg
     1081 ttgcaccatg gtctgtagca taaaccgact gggatactga aaaaacgata ttgtccgagt
     1141 tgttggcggt tatgctatcc tgatttgttg aggaggttat acctatggtg tagtttgtat
     1201 tttcagtaat tttagcctgt ccgttgagct tagctaaacg ttgctcacct tcgtttaagg
     1261 tatcggtgat agtggcaaaa gcattaatat caggaagttg ctccgatgtg gcaacaattt
     1321 caatggtgtc gctaat
//
LOCUS       DAOOQX010000623         1333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_135832_length_1333_cov_2.904538, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000623 DAOOQX010000000
VERSION     DAOOQX010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135832_length_1333_cov_2.904538"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLX53_08980"
     CDS             <1..349
                     /locus_tag="PLX53_08980"
                     /inference="COORDINATES: protein motif:HMM:NF022059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOY73255.1"
                     /translation="PRKKFKDTIIGFEGVPTGGSMMLFNSSRSMYNVLKNYLDFFSEE
                     SCGQCTPCRVGCQQLILGIEAVKKGDRHAAYLDQLQKLAQTMKITAKCGLGQSVANSF
                     FSIVENFKEEMIY"
     gene            367..>1333
                     /locus_tag="PLX53_08985"
     CDS             367..>1333
                     /locus_tag="PLX53_08985"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOY73256.1"
                     /translation="MAKQINLTIDGFPITVEEGTTILQAAKKLNIHIPTLCYHDDLCV
                     AGNCRVCVVEMKGSKTLPASCAMPAAEGMEIFTNTIKVRTARKHIIDLLLSEHNAKCT
                     TCYKNGNCELQNLASEYKISEPMFIDLVPYKNYQMDAYSPSIIKDDSKCIRCQRCVRT
                     CEELQHVSALGVAYKGANMKISTFFENPMFEVVCTNCGQCVNRCPTGALVERNYVEEV
                     WDAIYNPNKHVVVQTAPAVRVALGEALGMKPGQIVTGKMVTALRRLGFDSVLDTDFSA
                     DLTIMEEGFELLGRLKKALVDKDSTVKIPMATSCSPGWIKFIEHTY"
BASE COUNT          374 a          297 c          317 g          345 t
ORIGIN      
        1 tccccgaaaa aagttcaagg ataccataat cggatttgag ggtgttccta ctggaggttc
       61 catgatgctc tttaacagct cgcgatccat gtacaacgtg cttaaaaatt acctggattt
      121 cttttccgag gagtcatgtg ggcagtgcac tccgtgtcgc gttggatgcc agcagcttat
      181 acttggtatt gaagcggtta agaagggcga tcgccatgct gcatacctcg atcagctgca
      241 gaaactcgcc caaacaatga agataaccgc aaaatgcggt ttggggcaaa gcgtggcaaa
      301 ttcgttcttc tctattgtgg agaactttaa ggaagagatg atttactaaa ttaaaatttc
      361 aaattcatgg ctaaacaaat aaacctcacc atagatggtt tcccaatcac tgttgaagag
      421 ggaactacaa ttctacaggc tgctaaaaaa ctaaatatcc acattccaac gctatgttat
      481 catgacgatt tatgcgtggc aggaaattgt cgtgtttgcg tggttgaaat gaagggatcg
      541 aaaacacttc ctgcttcatg cgccatgcct gctgcggagg gaatggaaat ttttacaaat
      601 acaattaagg ttcgcaccgc ccgtaagcac atcatcgacc tcttgctctc cgagcacaac
      661 gccaagtgca ccacatgcta caagaacggt aattgcgagc tgcaaaacct ggcctcggaa
      721 tacaaaattt ccgagcccat gtttatcgat ctggttccat acaaaaatta ccagatggac
      781 gcctattcac catcaataat taaggatgac agcaagtgca tccgttgtca gcgttgcgtg
      841 cgtacctgcg aagaactcca gcatgttagc gcactggggg ttgcatataa aggtgccaac
      901 atgaaaattt ccaccttttt cgaaaatccc atgttcgagg tggtttgtac caactgcggc
      961 caatgcgtaa accgctgtcc aaccggagcg ctggttgaac gcaactacgt ggaggaggtt
     1021 tgggatgcca tttataaccc taacaagcat gttgttgttc aaactgcacc ggctgtaagg
     1081 gttgcccttg gagaggcgct ggggatgaaa cctggtcaaa tcgttaccgg caaaatggta
     1141 accgccctta gaaggctagg gtttgattca gttttggata ctgatttttc agccgaccta
     1201 accattatgg aggagggatt cgagttgctt ggcaggctca aaaaggcatt ggttgacaag
     1261 gatagtactg tgaagattcc catggcaacg agctgctctc ccggttggat taagtttatt
     1321 gaacatacct atc
//
LOCUS       DAOOQX010000624         1332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_136046_length_1332_cov_2.393892, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000624 DAOOQX010000000
VERSION     DAOOQX010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136046_length_1332_cov_2.393892"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /gene="hydG"
                     /locus_tag="PLX53_08990"
     CDS             <1..957
                     /gene="hydG"
                     /locus_tag="PLX53_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOY73257.1"
                     /translation="HRSPGYGVKKDRGEIRRVNINAAPLSVEDFLKVKKAGIGTYQIF
                     QETYNPEAYSWYHQAGKKRDFEWRLTSLDRAQEAGLDDVGIGALMGLYDWRFEVLGLV
                     RHTNHLEACYNVGPHTISFPRIKSASGLDITNQYFVSDDDFVKLVAILRLAVPYTGMI
                     LTAREPVHVRQKVLQFGVSQIDGGTKLELGAYIESKNEKQNLNREQFEIGDSRSLGEV
                     VDDLVEHDYIPSFCTACYRLGRTGEHFMEFSVPGFIKRYCTPNAVLTLTEYLVDYATP
                     EVKDKGYRLIDKTLTELDGNQNVEEIKRRMERIKAGERDLYF"
BASE COUNT          355 a          266 c          334 g          377 t
ORIGIN      
        1 caccgttcgc ctggttacgg ggttaaaaag gatagaggtg aaattcggcg ggtaaacatt
       61 aatgctgcac cgctaagcgt tgaagatttt ttgaaagtga aaaaggcagg gattggcacc
      121 taccagattt ttcaggaaac atacaaccct gaagcttata gttggtacca ccaggcaggt
      181 aaaaagcggg attttgaatg gcggctcacc tcgctcgata gggcacagga ggctggtttg
      241 gacgatgttg gaattggtgc attgatgggc ctgtatgact ggcgttttga ggtgttgggg
      301 ctggttaggc ataccaacca ccttgaggct tgttacaacg ttggccccca caccatatct
      361 tttccgcgta taaagtcggc ttcaggtctg gatattacca accagtattt tgtttctgac
      421 gatgactttg tcaagttggt ggcaatcctg cggctagctg tcccctacac cggcatgatt
      481 cttaccgctc gtgaacccgt gcatgttagg caaaaggttt tgcagtttgg cgtctctcaa
      541 attgatggag gaactaagct ggagctgggc gcttatattg agtctaaaaa cgagaagcaa
      601 aatcttaatc gtgagcagtt tgaaattggc gacagccgat ctttgggcga ggtggttgac
      661 gatttggtgg aacatgatta tatcccctcg ttttgcacgg catgttaccg cctggggcga
      721 accggcgagc attttatgga gttttctgta ccagggttta tcaaacgtta ctgtacccca
      781 aatgcagttc tcaccctaac cgagtatctg gttgactatg ctacacccga ggttaaggat
      841 aaggggtaca ggttgattga taaaaccttg accgagctgg atggaaatca gaatgtagag
      901 gaaataaaac gccgtatgga gcgaataaag gcaggggaga gggatttata cttttagcaa
      961 aggttccttc cgtttttact agaattatgt gatgcacagc ttataacctt gtatagccca
     1021 tctaccttaa tagcagatgg gtttttttat gtaaagtgtt aaccatagct gtcagctaat
     1081 cccattcatt ttgcgcgtag caagtgcggt tttaggaacc tgccatctgt ccacgacgcg
     1141 aatcggagta taacatcaac ccatctaaaa ataagcaaga tatgtagaac tctggaaaaa
     1201 ttgtagagaa ttaatctgaa aggcttaaaa atggttgaat tggctattta aacgttatta
     1261 gaactcaccc tttttttttg ccagattttt caccttgcca atttccataa aggttgcgcc
     1321 attgctcggg cc
//
LOCUS       DAOOQX010000625         1331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_136175_length_1331_cov_2.820533, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000625 DAOOQX010000000
VERSION     DAOOQX010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136175_length_1331_cov_2.820533"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PLX53_08995"
     CDS             complement(<1..534)
                     /locus_tag="PLX53_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOY73258.1"
                     /translation="MRNSKNFTGHQLLMAILLAFILVFSSCKYSAKEEIPFQVQHADW
                     VYNATIYEVNVRQYTPEGTFKAFESHMPRLKELGVEILWFMPIHPIGEVNRKGALGSY
                     YSVKDYYGVNPEFGTLDDFKSVVAQAHQLGMKVIIDMVANHTSHDAVLVKEHPDWYVH
                     DSLGNLVSPFDWTDVAKL"
     gene            830..>1331
                     /locus_tag="PLX53_09000"
     CDS             830..>1331
                     /locus_tag="PLX53_09000"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOY73259.1"
                     /translation="MNRVLVATGGGDCPGLNAVIRAVVKRASQEPDWEVIGSIQSFDG
                     ILREPTEIRVLDEKAVSGIHVQGGTIIGTTNKGGPFAWPIKNKDGSWGAVDRSDEMIR
                     KLQYLGIDAVISIGGDGSQKISQQLYEKGLNIIGVPKTIDNDLSATDFTFGFQTAVQI
                     ATDAVDK"
BASE COUNT          417 a          252 c          302 g          360 t
ORIGIN      
        1 aagcttagca acatctgtcc agtcgaatgg tgaaacaagg ttccccaagc tgtcgtgaac
       61 gtaccaatcg ggatgctcct tcaccagcac ggcatcgtgg gaggtgtggt tggctaccat
      121 gtcaattatt accttcattc ccagctggtg agcttgggca acaaccgact taaagtcgtc
      181 gagggtacca aactcagggt taactccata gtagtcctta acggaatagt agctaccaag
      241 cgctccttta cggtttacct cgccaatggg atgaataggc ataaaccata gaatctcaac
      301 cccaagttct ttcagcctgg gcatgtggct ttcaaaggcc ttaaaagtac cctcgggagt
      361 gtactgccta acattaacct catagatggt ggcattgtaa acccaatcgg catgttgtac
      421 ctgaaagggg atttcttcct tggcgctgta cttgcacgaa gagaaaacca gaataaaagc
      481 aagcaaaata gccattagta gctgatggcc tgtaaaattt ttagaatttc tcatagatgt
      541 aaattttagt agggttactt ataacttatc attatattcc caaaaaaatg aaaaaatgaa
      601 aaggaaaacc atcaaatttc aaacgattgc aacatggcgt acttaataaa tacagtagca
      661 ttaagcctca tagcaaagat aacattgttt ttttttatac ttttgcaaaa gtgggaaaaa
      721 ttggttttcc agcactgaaa cttataggtg gaaccatgga agtccatgtt taacctgaac
      781 cagattatga gatgttctgt ataacaataa gataactaaa ttttagcaga tgaatagagt
      841 tttagtagcc actggaggcg gcgattgtcc tgggttaaat gcagtgataa gggcggttgt
      901 aaagcgagcc tctcaggaac ccgactggga ggttattgga agcattcagt cttttgatgg
      961 aatacttcgt gagccaacag aaataagagt attggacgaa aaggcagtat cagggattca
     1021 tgttcaagga ggaactatta tcggtacaac caataaggga ggcccatttg catggcctat
     1081 taaaaacaaa gatggttctt ggggtgctgt tgaccgttcc gatgaaatga ttaggaagct
     1141 gcagtattta ggtattgatg ctgtaataag tataggaggt gatggttcgc agaaaatatc
     1201 gcagcaactg tatgaaaagg ggctaaatat tattggtgtt cccaaaacca tagataacga
     1261 cctttcggcc accgatttca ccttcggatt tcaaactgcc gttcaaattg ctacagatgc
     1321 ggttgataag c
//
LOCUS       DAOOQX010000626         1331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_136271_length_1331_cov_1.721787, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000626 DAOOQX010000000
VERSION     DAOOQX010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136271_length_1331_cov_1.721787"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1027
                     /gene="metX"
                     /locus_tag="PLX53_09005"
     CDS             2..1027
                     /gene="metX"
                     /locus_tag="PLX53_09005"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOY73260.1"
                     /translation="MEGNFRIDTLPLECGQILKQVNIRYHILGDIGRNADKVVWICHA
                     FTANSNPAEWWPGIVGEGKYFDPDKTPVVCANILGSCYGSTGPKSVNPETNQPYLRNF
                     PLVTFRDVVRAHNLLRIHLGINRIWMLIGGSVGGYQAMEWECMFPGTTQHLVLIATAA
                     ISSPWVKAFSQSQRLALLADQSFVNLEVDGGRAGLVAARSIALLSYRGRSSYNITQQD
                     TCASRLEGYRACTYQTYQGEKLANRFDAYSYYTLSLMLDSHNMGRSRAGACEALASLR
                     ANTLVVGIDSDILFPVEEQIFLARHIPKARYVEIHSDFCHDGFLIENEQLSSAVSSYL
                     SCFGEVV"
     gene            1046..>1331
                     /locus_tag="PLX53_09010"
     CDS             1046..>1331
                     /locus_tag="PLX53_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73261.1"
                     /translation="MDTTIVRKTNKQAHKSYPITQQVIGLFGFGNVGKGLYHVLCHVP
                     REKAYIKTICVKDRSKKRDECANLITYNPDDILRDPDINLVVELIDSARDA"
BASE COUNT          345 a          291 c          343 g          352 t
ORIGIN      
        1 tattgaaggg aactttagaa ttgatactct acccttagag tgtggtcaaa ttttaaagca
       61 agtaaacata aggtatcaca tactaggtga cattggtaga aatgccgaca aagttgtttg
      121 gatatgccat gcatttacgg ccaacagcaa tccggccgag tggtggcctg gaattgtggg
      181 cgaagggaaa tacttcgacc ccgataaaac gcctgtggtt tgcgccaata ttctaggctc
      241 atgctacgga tcaacgggac caaaatcggt taacccggag acaaaccaac cataccttcg
      301 taattttcca ctggttacct ttcgcgatgt tgtccgagcg cacaacctgc ttaggattca
      361 cttggggatt aataggattt ggatgttgat tggcggttcg gttggaggtt atcaggcaat
      421 ggagtgggag tgcatgtttc ccggcacaac gcagcacttg gtgctaatag ccacagcagc
      481 gatttcgtcg ccctgggtta aagcgtttag ccagtcgcag cggcttgccc ttttggccga
      541 ccagtcgttt gtcaaccttg aggttgatgg tggaagagca gggttggttg ctgcccgcag
      601 catcgcattg cttagctata ggggtcggag cagctacaac attacccagc aggatacctg
      661 cgcttcccgg ctcgaagggt acagagcctg tacctatcaa acctaccagg gcgaaaagct
      721 ggccaaccgt ttcgatgcat actcatacta tacgttgagc cttatgctcg attcccataa
      781 tatgggcaga agccgagctg gagcttgcga ggcactcgct tctttacggg caaatacgct
      841 ggttgtgggc atagattccg atatcctttt cccggttgag gagcaaattt ttcttgccag
      901 gcatattcca aaagcccgtt acgtagaaat acattctgat ttttgtcatg atggtttttt
      961 aattgagaat gagcagcttt ccagtgccgt aagcagctat ctgagctgtt ttggcgaggt
     1021 ggtctagcta aaaatttgag ttgaaatgga tacaactata gttaggaaaa ctaataaaca
     1081 ggcccataag agctatccta taacccaaca ggttattggc ctatttggat ttgggaatgt
     1141 gggcaagggg ctataccatg tgctttgcca cgttccacgc gaaaaagcct acatcaaaac
     1201 aatttgcgtg aaggatagga gcaagaagcg ggatgaatgt gccaacctga ttacctataa
     1261 tcctgatgat attctccgcg atcccgatat taacctggtg gtagagctga ttgacagtgc
     1321 ccgggatgct t
//
LOCUS       DAOOQX010000627         1330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_136358_length_1330_cov_2.861961, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000627 DAOOQX010000000
VERSION     DAOOQX010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136358_length_1330_cov_2.861961"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PLX53_09015"
     CDS             complement(<1..896)
                     /locus_tag="PLX53_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOY73262.1"
                     /translation="MSEISLKVKELSEKYRDYTASNLSRLVRIKSLSTQEHDVQLELK
                     RQMEEAGFDEVRIDGLGNVVGRIGNGSRILAIDGHMDTVDIGYRNNWDFDPLGGEIRD
                     GFVHGRGSVDQKGGPASFVTSGRILKELGFDRDLTIYFVGSVMEEDCDGLCWKYIIEE
                     DGIKPDFVISTEPTNLNIYRGHRGRMEMHVQFYGVSAHGSAPERGKNAIYMASRAALE
                     IEKLNEHLKVDEFLGKGSVTISEIVSGSPSLCAVADYARIHLDRRLTWGETMETAVGE
                     VEEIVKNMNAKVEVLNYSEKAYT"
BASE COUNT          334 a          348 c          262 g          386 t
ORIGIN      
        1 gtgtaggcct tctcggagta gttcagcacc tcaaccttgg cattcatgtt tttaacaatc
       61 tcctccacct cgccaacagc cgtttccatg gtttcacccc aggtcagcct gcggtcgaga
      121 tgaattcggg cataatcggc taccgcacat agcgacggac tccccgatac gatttcagaa
      181 attgtaacgc ttcccttgcc caaaaattcg tcaaccttca ggtgttcgtt gagcttctca
      241 atctccagag cagcccttga ggccatgtag atggcatttt tgccacgctc aggagccgag
      301 ccatgggctg acaccccgta aaattggacg tgcatctcca ttcttcccct atgccctctg
      361 tagatattta ggttagttgg ttcggtgctg attacaaaat cgggtttaat cccatcctcc
      421 tcgatgatgt acttccagca tagcccgtcg caatcctcct ccattacgct gccgacgaag
      481 taaatggtga gatcgcggtc gaagcccagt tctttcagta ttcgtcctga agttacgaat
      541 gaggctggac cacctttctg gtcaacagag ccccttccgt gcacaaaacc atcgcgaatt
      601 tcacctccta acgggtcgaa gtcccagttg ttgcggtaac caatgtcaac ggtatccatg
      661 tggccatcta ttgccaatat cctactaccg ttgcctattc ggccaactac attccctaac
      721 ccatcaatcc ttacctcatc gaagccggct tcctccattt gtctctttag ctccagctga
      781 acatcatgct cctgggtgct tagggatttt atcctgacca aacgcgacaa gttactagcc
      841 gtatagtcgc ggtatttctc ggaaagttcc ttaaccttca agctaatctc actcatgatg
      901 attatcctat tttggttcaa aattaccgga tatatcaata gccacatcaa gttttagttg
      961 taaattatac attttattca aatttagtat ttttctcata gtattaactt acattatagg
     1021 tattttttct atatataaag gctaactaaa ggatggttaa tatagctgta ccatgaaaat
     1081 ggttaaatat taaaggaatt cagggcccgt caaactctga cggagcttgt gtaactcttg
     1141 gcggagaaaa gttaaatgtt acgcggtcgg gaaccgagcc gttttttggc gagttcgccg
     1201 aaggcaaacc gctacttaaa tacgacgaag ttacgctttg ctaaacttcg ccgagagggg
     1261 gttaaccttt tcccgttctg cctctcaacc agagcaccta gtactcccat ccttaaattc
     1321 ataaagcata
//
LOCUS       DAOOQX010000628         1328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_136673_length_1328_cov_3.498036, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000628 DAOOQX010000000
VERSION     DAOOQX010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136673_length_1328_cov_3.498036"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PLX53_09020"
     CDS             complement(<1..861)
                     /locus_tag="PLX53_09020"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOY73263.1"
                     /translation="MKRLAILLLFSILTPIQNFGQTVSLVLSGGGAKGCAHIGVIKAL
                     EENGIPIDNVSGTSIGAIVGGLYAMGYSPDEMVNIFKSRDFYNWSNGLIDESYKFNIN
                     DLSTTDAENLSVGFTFDRGGLKTKLTSSIVPPVGLDIAFEEMFAQGTAISRGNFDKLF
                     VPFRCNASDVVNKKQVYFREGNLGKAIRTSMTFPLYFTPVYIDSLLLFDGGIYNNFLW
                     KEALQEFHPNIIVGSKVASNSKLPSDEDPLLQVEAMIVGITDYSIPDSLGIVIDTRLE
                     NIELLDFDKVD"
     gene            complement(1108..>1328)
                     /locus_tag="PLX53_09025"
     CDS             complement(1108..>1328)
                     /locus_tag="PLX53_09025"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOY73264.1"
                     /translation="YLFTDGYVDQFGGPEGKKFKFRRFRRLLLNIYRLPLETQQKILE
                     ESITEWMGGQEQVDDILVIGIRTPTKAK"
BASE COUNT          377 a          324 c          261 g          366 t
ORIGIN      
        1 atcaacctta tcgaaatcaa gtagctcaat attctccaac cgggtatcaa taactattcc
       61 caacgaatcg ggaatgctat aatcggtaat gccaacaatc atagcctcga cttgaagcaa
      121 ggggtcttca tcgctgggca gcttgctgtt agaggccacc ttactcccca caataatgtt
      181 gggatgaaac tcctgcagcg cttccttcca taaaaaattg ttgtatattc ccccatcaaa
      241 cagtagtagg gaatcaatat aaactggcgt gaagtaaagc gggaatgtca tggatgtccg
      301 gattgcttta ccaaggttac cctctctaaa gtaaacctgc ttcttgttta ccacatcgga
      361 ggcgttgcac ctaaaaggaa caaaaagttt gtcaaaattt cctctggaaa tcgccgttcc
      421 ctgagcaaac atttcctcaa aggcaatatc cagccctacc ggcggtacta tgctggaggt
      481 aagcttagtt tttaacccgc ctctgtcaaa ggtaaacccc acgctcaggt tctcggcatc
      541 ggtagttgac aaatcgttga tgttaaactt atagctttca tcaatgaggc cattgctcca
      601 gttgtaaaaa tctctagact taaaaatatt aaccatttca tcgggtgaat agcccattgc
      661 gtataggcca ccaacaatcg cccctattga tgttcccgaa acattatcaa taggaatccc
      721 gttttcctcc agcgctttaa ttacaccaat gtgggcacat cctttggctc ctcccccgct
      781 aagcacaagg ctaactgttt ggccaaaatt ctgaatggga gttaaaatcg agaacagcaa
      841 caggattgct aatcgtttca tccttaccaa ttttttagat ttctgacttc tatacccttt
      901 aggcatcggg tttaaatata tggaacatcc atttttggct tcgtcgaacc cgcaagtaac
      961 gcccggttgt tcatacgtgt ttgtgctagc cgtcctacat gggtgcttca taactagaaa
     1021 caaattagaa tcgtttagat ggcattaaaa gggttagatg atcaacatgc cgcctcaggc
     1081 aagtagctag cagcttaacc ctacatgcta ctttgcctta gtaggagtac gaatgccaat
     1141 aaccagaatg tcgtcaacct gttcctgacc gcccatccat tccgtgatac tttcctcaag
     1201 tattttctgc tgagtttcaa ggggtagcct ataaatgttg agcagcaggc ggcggaagcg
     1261 acgaaactta aattttttcc cctcgggccc accaaactgg tcaacgtaac catcggtaaa
     1321 aaggtata
//
LOCUS       DAOOQX010000629         1328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_136721_length_1328_cov_2.619010, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000629 DAOOQX010000000
VERSION     DAOOQX010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136721_length_1328_cov_2.619010"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /gene="lpxB"
                     /locus_tag="PLX53_09030"
     CDS             complement(<1..984)
                     /gene="lpxB"
                     /locus_tag="PLX53_09030"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOY73265.1"
                     /translation="MRYFIVAGEASGDLHASNLMRGIKAKDSSAEFRFFGGDLMKAQG
                     GTLVKHYRDMAFMGFLEVLLNLRTISGNMRLCKDEILAFKPDVLILVDYPGFNLRIAR
                     FAKKNEIRVFYYIAPKVWAWNESRVKVLKKYVDTLFIIFPFEQEYFARHGINAVYLGN
                     PTPDAIEQKIGGKVVTFNQFVEDNKLSQKPIIALLAGSRKQEIRYNLPVMLRMVEKFP
                     DYQLVIVGAFSLSEDVYRPYVAGKNVAIVFDQTYQLLSCSTLALVTSGTATLETALLN
                     VPQVVCYRGNILSMLVAWVVIRVKYISLVNLNMQAEVVKELKQFDLTPENLY"
     gene            complement(995..1291)
                     /locus_tag="PLX53_09035"
     CDS             complement(995..1291)
                     /locus_tag="PLX53_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73266.1"
                     /translation="MNSAKLNRQRVGYLFGILLPLLVLVVIFFTMYTGKSFWAFLQIM
                     WIQGNLSQVLSLAVIPNLAIFFIFIWTKKLKTAQGVLGATFILAFLIIILRFAR"
BASE COUNT          377 a          318 c          290 g          343 t
ORIGIN      
        1 gtaaagattt tcgggagtta aatcgaactg cttaagctcc tttactacct cagcctgcat
       61 gttcaggtta accaacgaaa tgtactttac ccgaataacc acccaggcta caagcatcga
      121 aagaatgttg ccccggtagc aaaccacctg aggcacgttg agcagagcgg tttcgagggt
      181 tgcagtaccc gaagtaacca gcgccagagt agagcagctt aacagctggt aggtctggtc
      241 gaaaacaatg gctacgtttt ttcctgcaac gtagggccga tatacatcct cgcttaacga
      301 aaatgctccg actatcacaa gctggtagtc gggaaacttc tccaccattc tgagcataac
      361 cggtaggtta taccttattt cctgcttacg acttccggca agtagggcaa ttatgggctt
      421 ttgagatagc ttattatcct caacaaactg gttgaaggtt acaaccttac cgccaatttt
      481 ctgctcaatg gcatcgggag ttgggttgcc caggtaaact gcatttattc cgtgtctggc
      541 aaaatactcc tgctcaaaag ggaaaataat gaagagcgta tctacgtatt tttttagaac
      601 tttaacgcgc gattcgttcc atgcccacac cttgggggca atgtagtaga atacccgtat
      661 ttcgttcttt tttgcaaagc gtgctatccg aaggttaaac ccggggtaat caaccagtat
      721 aagcacatcg ggcttaaagg caagaatctc atccttacaa agcctcatgt tgccggaaat
      781 agtgcgaaga ttcagcagta cctccaggaa gcccatgaag gccatatctc ggtaatgctt
      841 taccagtgtt cctccctgag ccttcatcaa atcgccacca aagaatctga attcggccga
      901 agaatctttg gccttaattc cccgcatcag gttggaggcg tgcaaatcgc ccgatgcttc
      961 tcctgcaact ataaagtagc gcatttagct ggttttatcg tgcaaaacgt aaaataatga
     1021 tgagaaatgc aagtataaat gttgccccca gcacaccctg ggcagtcttc aatttttttg
     1081 tccaaatgaa tatgaagaaa atagccaggt tgggaataac agcaagcgac agcacctgcg
     1141 ataggttgcc ttgaatccac ataatctgga gaaaagccca gaagctttta ccggtgtaca
     1201 tagtaaagaa aatcaccacc agcaccagca ggggtagcaa aatcccgaag aggtaaccta
     1261 cccgttgtct atttagcttg gctgagttca ttttttacgg cattgtaaag gtcattctcc
     1321 cacccttg
//
LOCUS       DAOOQX010000630         1328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_136764_length_1328_cov_2.036135, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000630 DAOOQX010000000
VERSION     DAOOQX010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136764_length_1328_cov_2.036135"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1141)
                     /locus_tag="PLX53_09040"
     CDS             complement(164..1141)
                     /locus_tag="PLX53_09040"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOY73267.1"
                     /translation="MDLFGCRRPRLISVQLPVAIIIIHTLTLQQVVVDLAKRFISKVI
                     SRKSAAINCYFSAVVFALLALFVCHPTFAQVDRCSRDIPHLSANERITYKAYYNWKFI
                     WLRAADILFTSNDTSYNNVPAHHFLAEGRSLKEYDLFFKVRDRFESIVAKEGFRPLYF
                     IRDTYEGGYKAFNKYIYNYTGSSLYMETQTSERPFRIDIKPINTCTFDVLSAIHYCRG
                     LDFSSHSKGDIIPFAIAIDNEVFELYIRYQGRERLELRDGRIYNTVKFSVKLVEGTVF
                     KGGEDMVVWASDDPGHVPIMVEAKILIGSVKAILSTAENLAFPLLFEKK"
BASE COUNT          377 a          297 c          281 g          373 t
ORIGIN      
        1 cttccattcg gctaccagaa tataggattt agctacagga ttggccatag agtagccatt
       61 tttatatcgc ttaaaagcca tcttaacaat gccgaccaca tctatgccgg gttagcctta
      121 agcatctgaa aggtatttgc ccaactgtgg actatgcagc aagctatttt ttttcgaata
      181 acaaggggaa tgccagattt tcggcagtgc tgagtattgc tttaacagaa ccaatcagaa
      241 tttttgcttc aaccatgatt ggaacatgtc cagggtcgtc gctggcccaa accaccatat
      301 cctcacctcc tttaaaaacc gttccctcca ccagcttaac gctgaatttc accgtattat
      361 aaattcttcc atcgcgtagc tccagccttt cccttccctg gtaacgaata tatagctcaa
      421 atacctcgtt gtcaatagca atggcaaagg gtattatgtc gcccttgcta tggctcgaaa
      481 aatccaagcc cctgcagtag tgaatggcac ttaaaacatc aaaggtgcat gtgttaatgg
      541 gtttaatatc aatcctgaag ggtcgttcgg atgtctgggt ctccatgtac aaggagctgc
      601 cggtatagtt atagatgtac ttgttgaaag ctttatagcc tccctcgtag gtgtcgcgaa
      661 tgaagtaaag gggtcgaaaa ccctccttgg ccacaatgga ttcgaacctg tcgcgaacct
      721 tgaaaaaaag gtcgtactcc tttaggctcc gcccttcggc aagaaagtga tgtgcaggca
      781 cattattata ggaggtatcg tttgaggtaa aaaggatgtc ggctgctcta agccatataa
      841 acttccagtt atagtaagcc ttgtaggtga tgcgctcgtt ggcgctgaga tgtggaatgt
      901 ccctggagca tctatcaacc tgggcaaatg tggggtggca tacaaaaagt gcaagcaatg
      961 caaacaccac tgcactgaaa tagcagttga tagccgctga tttccttgaa attacttttg
     1021 agatgaatcg ttttgccaag tcaactacta cttgttggag tgttagcgta tgaataataa
     1081 taatggcaac tggtagctga acgctgatta gccttgggcg cctgcagcca aataaatcca
     1141 tggaacttca gctgcaaatt ttttaaatca acagacaaag gacaatcatt taccctataa
     1201 catccaccag gctcccataa ttccaatgca ggccatacaa taagtgcatg atcctatact
     1261 ttcttgtatg accttatcat tgggattaaa tatttaccaa agatctactt aagttgggct
     1321 attgctgc
//
LOCUS       DAOOQX010000631         1327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_136912_length_1327_cov_2.234277, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000631 DAOOQX010000000
VERSION     DAOOQX010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136912_length_1327_cov_2.234277"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..976)
                     /locus_tag="PLX53_09045"
     CDS             complement(107..976)
                     /locus_tag="PLX53_09045"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOY73268.1"
                     /translation="MENKMTRFFKRISANPYISTILLGIGLFVVLLIVLGISLSIYTR
                     HGQTFAIPDFRGESVDKLPELVRKAGLRMEVTDSVYIFNRAPGSIIDQNPKPGTHVKK
                     NRRVFVTINAKNPIKVEVPDVKGYTLRQAKAILEQQGFEVGKLSFTPDMGVNNVLDQL
                     YKGKPAIPGMLIPKGSKIDLLLGMGMHGERTGLPLLIGVKFEEAKNRIIEASLNVGRI
                     KFDETIETYRDSLEARVYSQYPAYSEAISLSFGTRVDIWLTLNESRIPAIKSFNADSI
                     PAVGSYNGEDIIE"
     gene            complement(1133..>1327)
                     /locus_tag="PLX53_09050"
     CDS             complement(1133..>1327)
                     /locus_tag="PLX53_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HOY73269.1"
                     /translation="TRLAIAFGADDIDGTIDDTTKIYSMAGAEEQKPAMTVEKLTQLI
                     RESGKVPVERNSLYHAIKYY"
BASE COUNT          371 a          305 c          285 g          366 t
ORIGIN      
        1 agaaagattt accaatgtat cctgagcttc caccgacatt acgtggagag tcatcattgc
       61 caaaaaggag gaatgcctga tacctttcat gaagattttg aagtagctac tcaattatgt
      121 cctccccatt atagctgcca acggcaggaa tagagtcggc attaaagctc ttaatagctg
      181 ggatacgcga ctcgtttagg gtgagccata tatccacccg tgttccaaaa ctaagagaaa
      241 ttgcctcaga gtatgcagga tattggctgt aaaccctagc ctccaacgaa tccctatatg
      301 tttcgatggt ttcatcaaac ttaatgcgac caacgtttag cgaggcctca ataatcctat
      361 tcttggcctc ctcaaactta actccaataa gcaacggaag gcctgttcgc tcgccgtgca
      421 ttcccatacc aagcagcagg tcaattttag agcctttggg gatgagcatt ccggggatgg
      481 ctggtttgcc tttataaagc tggtcgagca cattgttcac tcccatgtcg ggtgtaaagg
      541 acagctttcc aacctcgaat ccctgctgtt ctagtatggc cttagcttgg cgcagggtgt
      601 accctttcac atcgggtact tcaactttaa tgggattttt agcattaatg gtaacgaata
      661 cccgcctatt cttcttcaca tgagttccgg gcttaggatt ctgatcgata atgctcccgg
      721 gggcgcggtt gaagatgtaa accgaatcgg taacctccat tcttagcccc gcttttctga
      781 caagttcggg caacttgtca accgattcac ctctgaaatc aggaatggca aaggtttgtc
      841 catgccgggt gtatatgctt agcgaaatcc ctaacactat cagcaataca acaaaaagcc
      901 caatacccag cagaatagtt gaaatgtagg gattggccga aattctctta aaaaaacgtg
      961 tcatcttatt ctccataacc gtaggtgtgg aagtggcaaa tttaacacta ttgcagcaac
     1021 aaaaaagcgt ttgctaccgc cgattttcta ataaccagat cctagcaaaa agtagcaacc
     1081 atgagcatga aaaaatcatt gtataaaaat ttgtcaattt agataactgc acctagtagt
     1141 attttattgc atggtaaagc gaattgcgtt caacgggaac tttgccggat tctctgatta
     1201 gctgggtaag tttttcaacc gtcatggcag gtttttgctc ctcggcacca gccatggagt
     1261 atattttggt ggtatcgtca atagtgccat caatgtcatc ggctccaaaa gcaatggcca
     1321 aacgggt
//
LOCUS       DAOOQX010000632         1323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_137588_length_1323_cov_2.621451, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000632 DAOOQX010000000
VERSION     DAOOQX010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137588_length_1323_cov_2.621451"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1323)
                     /locus_tag="PLX53_09055"
     CDS             complement(<1..>1323)
                     /locus_tag="PLX53_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73270.1"
                     /translation="FFIKEASRGTTLQNSLLHQDVEKELPEKANYRIYTVPSKAKRLL
                     GNYLSNRMATMLTSSPTGKTGALACQLAGGNRNIVATLTVNPSVKEQVAALPVEWVCQ
                     LSNTPTLMPQVVVNHNTGEKEVLVQDKANTLYLIDRFGKTIWKRTVDGQVIGNVTQVD
                     IFNNRKLQLAFSTSSKLYVIDRNGKDVQGFPVIFQSRATNPLMVVDYEGNHSYRFFQA
                     CSDRKIYVFDSYGKIVPGWMFGSTETIVSERIGYARVAGLDYIIVFDQNRPYILNRKG
                     EERVKPERFFAKNPNSHYALGKDEKGSYFVVTSDTKGLIRFMYLNGKVKDMALRHFTS
                     SHSFGYFDVTSDGQDDFVVADGEYLLATDHLGKKIFSTRFKTKLQPELYYFKLEQIMG
                     IGVRAASNDENYLVDRSGEIVTGFPIEGCTMFSVAKLSNLSKMYNLIVG"
BASE COUNT          333 a          303 c          293 g          394 t
ORIGIN      
        1 ccaactatca ggttgtacat tttggacaag ttgctcagct tggcaactga aaacattgta
       61 cacccctcaa tgggaaagcc tgttactatc tctcccgatc tgtcaaccag gtagttttca
      121 tcattacttg ccgctctaac accaataccc ataatttgtt caagtttaaa gtagtaaagt
      181 tcgggttgca atttagtttt gaagcgggtg gagaatattt tctttcccag atggtcggtg
      241 gcaaggaggt actcaccatc ggcaacaacg aagtcgtctt ggccatcgct ggtaacatca
      301 aaatatccaa aggaatgcga tgaggtgaaa tgacgcaatg ccatatcttt aaccttaccg
      361 ttgaggtaca tgaacctgat taagcctttt gtatccgatg taactacgaa gtagcttcct
      421 ttctcatctt ttcccagggc gtaatggcta ttggggtttt ttgcaaaaaa cctttcgggt
      481 ttaacccgct cctcgccctt tcggttaagt atatatgggc ggttttggtc aaaaactatg
      541 atatagtcta gtccggcaac tcttgcgtaa ccaattcgtt cactgactat agtttccgta
      601 ctaccaaaca tccatccggg aacgattttt ccataggaat cgaatacata aatttttcgg
      661 tcgctgcaag cctgaaagaa ccggtagcta tggtttccct catagtcaac caccatgagg
      721 ggattggtgg ctctggattg gaaaatgacc ggaaatcctt gaacatcctt cccgttgcgg
      781 tcaataacgt aaagcttgct gctggtgcta aatgccagct gtagcttgcg gttgttgaaa
      841 atatcaacct gggtaacatt accaataact tgcccgtcaa cagtacgttt ccagattgtt
      901 ttcccaaagc ggtcaataag gtaaagcgtg ttggccttgt cctgaacgag cacttccttt
      961 tcgccggtgt tatggttaac cacaacctgt ggcataaggg ttggtgtgtt gctgagctgg
     1021 caaacccact ccactggtaa tgctgcaact tgttctttta cagagggatt aactgtaagg
     1081 gtagccacaa tatttcggtt accaccggcc agctggcatg ccaatgcccc ggttttacct
     1141 gttggggatg aggttagcat ggtagccatc ctgttgctta aatagttgcc taatagcctt
     1201 ttcgctttag aaggaactgt ataaatacgg tagttcgcct tttccggcag ctccttttcc
     1261 acatcctggt gtaacaagct gttttgaagg gttgttcccc gcgacgcttc cttaatgaaa
     1321 aat
//
LOCUS       DAOOQX010000633         1322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_137738_length_1322_cov_3.051302, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000633 DAOOQX010000000
VERSION     DAOOQX010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137738_length_1322_cov_3.051302"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..>1322
                     /locus_tag="PLX53_09060"
     CDS             25..>1322
                     /locus_tag="PLX53_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY73271.1"
                     /translation="MDSKFSGPATYYYSHIAYLQKKYATALKGFEKLQNDETFAPIVP
                     FYISQIYYLQGDYQKVVAYAPPLVADNATRRTPEIARIVGDSYFKLRQYDSALVYLNK
                     YVEKTPQLSRDDYYLVGFTYYKTGNFSEAAKHLERVSTVDDSLSQNANYHLADCYLKL
                     NDKPKARQAFGMAAKLDFDSFIKEDALFNFAKLTYEQLYAPFNEAIEAFRQYITLYPN
                     TPRTDEAYNYLTLAYLSTRNYKEAVDALEKIKVQDVTIKTAYQKATFYRGLELFQNMQ
                     FDDAITMFAKSLENSAYNPTIAALATYWQAEAYYRLEQFEKAAEGYNRFILTSGAFSL
                     PEYLMAHYNLGYSNFKLKRYDEAVVWFRKFTNLSNDEKNVFVGDAYNRIGDSFFIQRR
                     YWAAIDYYDKAMAIGTIDADYALFQRGFALGLVERPEKKI"
BASE COUNT          403 a          332 c          267 g          320 t
ORIGIN      
        1 aagtggttat accaggtaaa ggacatggac agcaaatttt ccgggcctgc aacctactac
       61 tactcacaca ttgcctacct tcaaaaaaaa tatgcaaccg cactaaaggg ttttgaaaag
      121 cttcagaacg atgaaacgtt tgctcctatt gttccctttt acatatccca aatctactac
      181 ttgcagggcg attaccaaaa ggtagtggct tatgctcctc cactggtagc cgataatgcc
      241 accagacgaa cccctgaaat agcacgaata gttggtgatt catacttcaa acttcggcag
      301 tacgacagcg cgctagttta cctgaacaag tatgttgaaa aaactcccca gctatcgcgc
      361 gacgattact acctggtagg atttacctac tataagacag ggaatttttc tgaagcagcc
      421 aagcaccttg agcgagtttc cacggttgat gactcgttaa gccaaaatgc caactaccac
      481 ctggccgact gctacctgaa gctaaacgac aagcctaaag ccaggcaagc ttttggaatg
      541 gctgcaaagc tagatttcga ctcttttatc aaggaggatg cactttttaa ctttgccaag
      601 ctcacctacg agcagcttta cgcacccttc aacgaggcaa ttgaagcatt caggcagtac
      661 atcacccttt acccaaacac tccacgtact gacgaagcct ataactactt aaccttggcc
      721 tacctaagca caagaaacta caaggaggcg gtagatgcgc tcgaaaaaat caaagtgcaa
      781 gacgtaacca ttaaaaccgc ataccaaaag gcaacattct acagaggatt ggagctattc
      841 caaaacatgc aattcgacga tgccattacg atgtttgcaa aatcgctcga aaattcagcc
      901 tataacccta caattgcagc gctggcaacc tactggcagg ccgaggctta ttatagacta
      961 gagcagtttg aaaaagcagc tgagggctac aaccgattca ttttaacttc cggtgccttc
     1021 agcttacccg agtacctaat ggcacactac aaccttggtt attccaactt caaacttaaa
     1081 cgttacgatg aggccgttgt gtggtttcgc aagtttacca accttagcaa cgatgagaaa
     1141 aacgtttttg tcggcgatgc ctacaacaga atcggagatt catttttcat tcagcgtcgc
     1201 tactgggctg ccatagacta ctacgataaa gccatggcta ttggcacaat tgatgccgat
     1261 tatgccctgt tccaaagagg cttcgctctt gggctggtcg aaaggcccga aaaaaagatt
     1321 ga
//
LOCUS       DAOOQX010000634         1319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_138215_length_1319_cov_2.816456, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000634 DAOOQX010000000
VERSION     DAOOQX010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138215_length_1319_cov_2.816456"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..>1319
                     /locus_tag="PLX53_09065"
     CDS             73..>1319
                     /locus_tag="PLX53_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOY73272.1"
                     /translation="MGPKRAELLQKELNISTFGDLLYFFPFRHIDRSKIYKISEINED
                     LPYIQIVGRILRIEEVGVGRNRRLTAYLSDGSGSVELVWFRGVSWMAKWLKPNIEYLV
                     FGKANVFGGHFSIAHPEMEEYKTGQEKIMGTLQPVYSTTEKLKSNRITSKTILGLQQT
                     LIQAVNGKIEETLPPYLIEQQKLMTLPEALRNIHFPQSVLLLRKAEHRLKFEELFFIQ
                     LDILRKQTIRQSKINGHLFSRVGEMFNRFYSERLPFPLTNAQKRVIREIRRDMGSGKQ
                     MNRLLQGDVGSGKTIVALMSMLIAVDNGFQACLMAPTEILANQHFDSITSLLGDLPVT
                     VGLLTGSTRQSERNTLHKSLEAGELQIIIGTHALIEDVVKFKNLGFVVIDEQQRFGVA
                     QRAKLWEKNNNPPHVLVMTATPIP"
BASE COUNT          371 a          289 c          310 g          349 t
ORIGIN      
        1 aagtcggaca agctctggaa tttaacgtat atggctgatt tgattttaaa tgatataaaa
       61 tttctaccgg gtgtggggcc taagcgcgcc gaactactcc aaaaggagtt gaatatcagc
      121 acatttggcg atttgctcta tttctttccc ttcaggcata ttgaccggtc taagatttat
      181 aagatctcgg agataaatga ggatttaccc tacatccaaa tagttggtcg aatacttcgg
      241 attgaggagg ttggtgtggg gcgcaacagg cggctgacag cctatctgag cgatggaagt
      301 ggaagtgtag agctggtatg gtttcggggt gtttcatgga tggcaaagtg gttaaaaccc
      361 aacatcgagt acctggtatt tggcaaggct aatgtatttg gaggtcattt cagcattgcc
      421 caccccgaaa tggaggagta caaaaccggt caggagaaaa ttatggggac cctgcaaccc
      481 gtttactcca ccaccgaaaa gctaaaaagc aacaggatta cctccaaaac catactggga
      541 cttcagcaaa cccttattca agctgtcaat ggaaaaattg aggaaaccct acctccctac
      601 ctgattgagc agcaaaagct catgacactt cccgaggctc tcaggaacat tcattttccc
      661 caaagcgtct tacttttgag gaaagccgaa catcggctta agtttgagga gcttttcttc
      721 atacagctcg atatccttag aaaacaaacc atacggcaaa gcaagatcaa cgggcatttg
      781 ttttcgcgag ttggtgaaat gttcaaccgt ttttacagcg aaaggctgcc attcccttta
      841 accaatgctc agaaacgcgt tattcgtgaa attcgtcgcg atatgggcag tggaaagcaa
      901 atgaataggc ttttacaagg agatgttggt agcggcaaaa ccatagttgc attgatgtcc
      961 atgcttattg ctgtcgataa tggttttcag gcatgcctca tggcacctac tgagatttta
     1021 gccaaccagc actttgactc cattacctct cttttaggag acttacccgt tactgttggg
     1081 cttcttacgg gcagcacccg ccagtcggaa cgcaacacgc ttcataaatc ccttgaggca
     1141 ggtgaactgc agataattat tggcactcat gcgcttattg aggacgttgt aaagttcaaa
     1201 aaccttggtt ttgtggtgat tgatgagcag caacggtttg gtgttgccca aagggcaaag
     1261 ctatgggaga aaaacaataa tccaccccat gtgctggtta tgactgccac cccaattcc
//
LOCUS       DAOOQX010000635         1315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_138958_length_1315_cov_2.713492, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000635 DAOOQX010000000
VERSION     DAOOQX010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138958_length_1315_cov_2.713492"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="PLX53_09070"
     CDS             <1..1187
                     /locus_tag="PLX53_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOY73273.1"
                     /translation="KYPNQFEATRTLLSGLKYIRAFGRTSISARGYYRKNFDRFELFR
                     ENPPSWYMGHNYHTTDVYGLKANISMLYGFGKTEVGAEIRREEILSSNLGEALSDSVR
                     VKGRNAWYTKGGNRNLLNIFASQTFYLKRATLSGGLQLAYNSSFEYIWCWGADISYSL
                     NNHSRFFASVNRSYRVPTFTDLYYSSPTNVGNPNLKPEFATTIEGGLKANRKAWSVQL
                     AAFYRQGSRIVDWVKEPTDEAWHTINHTSLNTTGVEVFVQADLSEIFRDAGSINFSYS
                     FTSAEKNEDALLDSYYAMDYLRHNLRLALNQQFFKSLTLNLCYHFMARAGEFQDFATS
                     QIRKYPGVHLADVRLTYSTTRKLDLFIDVSNIFNAHYMDVANVMQAGRWVGAGMNLNF
                     NL"
BASE COUNT          362 a          291 c          311 g          351 t
ORIGIN      
        1 caaaataccc caaccagttt gaggctacaa gaacgctgct aagcggactt aagtacattc
       61 gggcttttgg tcgaacctca atttcagcca gaggttacta ccgtaagaat tttgacaggt
      121 ttgagctttt tcgtgagaat cctccttcgt ggtacatggg gcataactac cacacaaccg
      181 atgtttacgg gttaaaggca aacatctcaa tgctgtatgg atttggcaaa accgaagttg
      241 gagccgaaat caggagggag gagattctta gcagcaacct gggtgaggca ctttcagatt
      301 cggtgagggt gaaaggccgt aatgcctggt acaccaaggg tggaaatcga aatttattga
      361 atatttttgc ctcccaaacc ttttacttaa aaagggctac cctgtcgggt ggactgcagc
      421 tggcttacaa cagtagtttc gaatacattt ggtgctgggg tgccgatatt tcctactcgc
      481 tgaataacca ttccaggttt tttgcctcgg tcaaccgctc atacagggta ccaaccttta
      541 ccgatctata ctacagcagt cctaccaatg ttgggaatcc aaaccttaaa cctgagtttg
      601 ccacaaccat tgagggcggg ctaaaggcca ataggaaagc gtggtcggtg cagcttgccg
      661 cattctaccg acagggaagc agaatagttg actgggtgaa ggagcccacc gacgaggcgt
      721 ggcataccat taaccatact agcctgaaca caaccggcgt ggaagttttc gtacaggctg
      781 atttgagtga aatttttagg gatgccggta gcataaattt cagctactcc tttaccagcg
      841 ctgaaaagaa cgaggatgct ctgctcgact catactatgc aatggattac ctacggcata
      901 acctgaggtt ggctttaaac cagcaatttt ttaagagcct tacgctaaac ctctgctacc
      961 attttatggc cagggcagga gagtttcagg atttcgccac ctcgcaaatc aggaagtacc
     1021 ccggtgttca tctggccgat gttaggctta cctacagcac cacgagaaag cttgatctgt
     1081 ttattgatgt ttcgaatatt tttaacgccc actatatgga cgttgccaac gtaatgcagg
     1141 ccggacgttg ggtaggtgca ggcatgaacc ttaactttaa cttgtaattc tgctttaaaa
     1201 atgcaagcaa agctgaacat gcggtatttt gaaatatctc gtgatgtttt ccttaaaaaa
     1261 tgatcgttga gttttgaaaa gtattcttga attaaggtaa ccgcatgata acccc
//
LOCUS       DAOOQX010000636         1313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_139334_length_1313_cov_2.527027, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000636 DAOOQX010000000
VERSION     DAOOQX010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139334_length_1313_cov_2.527027"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLX53_09075"
     CDS             complement(<1..219)
                     /locus_tag="PLX53_09075"
                     /inference="COORDINATES: protein motif:HMM:NF019644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HOY73274.1"
                     /translation="MDNQKDSPIYGIRPVMEALESGMKISRVVVKQGLSGSLVSELMV
                     LLKQRNVPVQYVPLEAFDKFKDKNHQGVV"
     gene            complement(231..>1313)
                     /locus_tag="PLX53_09080"
     CDS             complement(231..>1313)
                     /locus_tag="PLX53_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOY73275.1"
                     /translation="RIKMLERMEIIEVDEDNTASVNIRFPSAPRSGDVVCEAKDVAKS
                     FGSKKIFGGVNFTIERGQKVAFVGRNGEGKTTMSRIITGELSHEGSLRIGHNVAIGYF
                     AQNQDELLDGSLTVLETIDRVAVGDIRTRMRDILGAFLFRGDDVDKKVSVLSGGERNR
                     LALAKLMLQPYNLLVLDEPTNHLDMHSKEVLKSALMQYDGTAILVSHDRDFLDGLVGK
                     VYEFSDGAVREHIGGIYDFLQRKKLESLKQLEHKARVDAQTTSSGNGSDLKRLWSERR
                     ELDREIRNMENSLKQVESKISAMENEIDDINRKLAEPDSHSINLSDSDFFAGYENKKR
                     QLLDYMQRWEELSLKLEELKLKRNAL"
BASE COUNT          304 a          364 c          279 g          366 t
ORIGIN      
        1 cacaaccccc tggtgatttt tatccttaaa cttgtcgaac gcttcaagcg ggacgtactg
       61 gacaggaaca ttcctttgct ttagcagcac catcagctcc gacaccaggc tgccgctcaa
      121 cccctgcttt acaactaccc tgcttatttt cattcccgat tccagagctt ccatcaccgg
      181 acgaataccg tagattgggc tgtctttttg attatccata gctgccagtg ttaaagtgca
      241 tttcgtttca acttcagctc ctccagcttt agggagagct cctcccagcg ctgcatgtaa
      301 tcaagaagct gccttttttt attttcgtag cctgcaaaaa agtcggaatc cgacaggttt
      361 atgctgtgtg agtccggttc ggccagtttg cggttaatat catcaatctc attctccatg
      421 gccgatattt tgctttcaac ctgcttcagg ctattttcca tgtttcgtat ttcgcgatca
      481 agttccctgc gctccgacca tagcctttta agatccgatc cattgccgga gctggtagtt
      541 tgagcatcaa cccgagcttt gtgctccagt tgtttcaggc tttccaactt cttacgttgg
      601 aggaaatcgt agattccccc aatgtgctca cgcacagcac catcggaaaa ctcataaacc
      661 ttgcctacaa gtccatccag aaaatctctg tcgtgtgaaa ccagtattgc cgttccatca
      721 tactgcatca gggccgattt gagcacctcc ttcgagtgca tgtcaaggtg gttggttggc
      781 tcgtcgagga caagcaggtt gtagggttgg agcatcaact tagccagggc caggcgatta
      841 cgctcaccac cggaaaggac gctgactttt ttatctacat catcacccct gaaaaggaaa
      901 gcgccaagaa tatcgcgcat cctggttcgg atgtccccaa ccgcaaccct gtcaatggtt
      961 tcgagaacgg tgagggagcc gtcgagcagc tcatcctgat tctgggcaaa gtagccaatg
     1021 gctacattat ggcctattct aagtgagccc tcatggctaa gctcgccagt tattattcgg
     1081 ctcatggtag tttttccctc cccatttcta ccaacaaaag ccaccttttg cccgcgctcg
     1141 atagtgaagt tcaccccgcc aaaaattttt ttggaaccaa agctttttgc cacatctttt
     1201 gcctcacaaa ccacatcgcc cgagcgggga gcagagggga agcggatgtt taccgaagca
     1261 gtattatcct catcaacctc aataatctcc atcctttcga gcattttgat gcg
//
LOCUS       DAOOQX010000637         1312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_139532_length_1312_cov_2.172633, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000637 DAOOQX010000000
VERSION     DAOOQX010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139532_length_1312_cov_2.172633"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PLX53_09085"
     CDS             <1..660
                     /locus_tag="PLX53_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73276.1"
                     /translation="LEPFSHARISFDSVPIVDIFILLVLAAAVVVILLAKRKNVLRKM
                     AAWASLSLTIAYFGTSAIIKTCVNGETRKSLITKGATYTRIHSTPLPLTNLIWMVVVE
                     DSSGYTISQKNLINGKTTHKAYLARNEGLLKCCENNNNMGKLLNFTRGFYTVEKSSNE
                     HLKIYDLRFSSLAGVYPQAFVFMFEVNCNDGKLLDHPAHPSRGIIPRNVKNLWNSLWQ
                     S"
     gene            complement(657..1070)
                     /locus_tag="PLX53_09090"
     CDS             complement(657..1070)
                     /locus_tag="PLX53_09090"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HOY73277.1"
                     /translation="MTPIKVFDSSYALVTFDIERKIIKVIWNGNHTVSEYKAAIGEAL
                     NFQRVSGFPVYTYLSDIRNQGIVNPESRKWFERVAIPKAVNGGLRRAAVVYDGSVFKN
                     YYLNLILLAANVYQLPLKFFGSMEEAYEWIESFHD"
BASE COUNT          403 a          293 c          263 g          353 t
ORIGIN      
        1 ctcgaacctt ttagccatgc cagaatttcg ttcgactcgg tgcccatcgt ggatattttc
       61 atcctacttg tactggcagc tgccgtggtg gtaattttac ttgcaaaaag gaaaaatgtt
      121 ctgcgtaaga tggctgcctg ggcgtcactt tccctaacca tagcctattt tggcacttcg
      181 gctataatta aaacatgtgt taacggtgaa accaggaaaa gccttataac caaaggtgca
      241 acctatacac ggattcatag cacgcctctc ccactgacaa acctaatatg gatggtggtg
      301 gttgaggata gcagcggata caccatcagc caaaaaaatc taattaacgg caaaactacc
      361 cacaaagctt acctggctcg aaatgaaggt cttcttaagt gctgtgaaaa caacaataac
      421 atgggtaagc tgctgaattt cacaaggggt ttctacactg tggaaaaatc aagcaatgaa
      481 catttaaaaa tttatgattt acggttctct agtcttgcag gcgtttatcc ccaagcattc
      541 gtattcatgt ttgaagttaa ctgcaatgat ggtaaattac tggatcatcc cgctcacccc
      601 agcaggggaa taatcccccg taatgttaaa aatctatgga acagcctatg gcagagctaa
      661 tcgtgaaaac tttcaatcca ctcataagcc tcctccatag agccgaagaa cttcaagggt
      721 agctggtaaa cgttagccgc cagcagaatc agattaaggt agtagttctt gaacacgcta
      781 ccatcataaa caacagcggc tctgcgtaac cctccattaa cggccttggg aatggctacc
      841 ctttcaaacc atttacggct ctcgggatta acaataccct gatttcggat atcggacaag
      901 taggtgtaaa ccgggaaccc gcttaccctt tgaaaattaa gggcttcgcc aatggctgcc
      961 ttatactcgc taacggtatg gttgccattc catataacct tgataatttt tcgttcgata
     1021 tcgaaagtta caagagcata ggaagaatcg aaaaccttaa ttggggtcat gttttttaag
     1081 taagttacca ccctacagct aaggagtcaa catcgattac acgaatatca tcaggccatc
     1141 ggtgtaaggc attttttaga tggtagaaag gaaactcatg agttggcccc gtcattccat
     1201 taccacattt ttacaatact atcccgtgtc aaaaattatt atagacaaaa ttaggctttc
     1261 aaaaacaagt atctctgggt aaaaattagg caactggagc aggatgccat tt
//
LOCUS       DAOOQX010000638         1310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_139807_length_1310_cov_3.255777, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000638 DAOOQX010000000
VERSION     DAOOQX010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139807_length_1310_cov_3.255777"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLX53_09095"
     CDS             complement(<1..762)
                     /locus_tag="PLX53_09095"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOY73278.1"
                     /translation="MSQVAKWLFRILAVAVFLLIAAAVVLPLVFKPQLKQLAKIEINK
                     NINATVEFDDFQVSLLRGFPNLFVGLKGVSIVGVERFAGDTLVSFGELGVRVNLWSII
                     DMEEIEVRSVYLSDAKVFARVTADGSKNWSIVKTSGLPQTEEEDTAAAKPTNIRIALT
                     RFKINNATFFYADDSLGMETTLRGVNLLISGNMVKRQTEIRLKSSADELDLRVKGIRY
                     IRDAEVRFNANMGVNLDNGTLSFRENELRINQLTLL"
     gene            1127..>1310
                     /locus_tag="PLX53_09100"
     CDS             1127..>1310
                     /locus_tag="PLX53_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73279.1"
                     /translation="MMKKIYLISFAAVAWLSACTTGEHKLATGPWLGVIQLDSTGGKL
                     DVPFNFELKNDSGTYSM"
BASE COUNT          392 a          293 c          258 g          367 t
ORIGIN      
        1 aagtaaggtt aactgattta tacgcagctc attctccctg aacgataggg tgccgttatc
       61 gaggttgacc cccatgttag cgttaaacct aacttcagca tcacgaatgt agcgtatgcc
      121 ttttaccctt aaatctaact catcggccga ggatttaagc ctgatttcgg tttgcctttt
      181 aaccatgttg cccgaaatca agaggttcac gccccgaagt gtcgtctcca tgccaagtga
      241 atcatcagca tagaaaaaag tggcgttgtt aattttaaac cgggttagtg caatcctgat
      301 gttggttggc ttagcggcag ctgtatcctc ctcctcggtt tgaggcaagc ctgaagtttt
      361 gacaatgctc caatttttgc tgccatcggc agtaaccctg gcgaatacct tggcatcact
      421 taggtaaacc gacctcacct caatttcctc catatcgatt atgctccata ggttcacccg
      481 tacgccaagc tctccgaacg atacaagggt atcgccagca aagcgttcaa caccaacaat
      541 gcttacccct ttaagcccca caaataaatt cgggaaacct cttaaaagcg aaacctgaaa
      601 atcgtcaaat tcaactgtag cgttaatatt tttattaatt tcgattttgg ccagctgctt
      661 aagctgaggt ttaaaaacca gtggtaacac cacagccgcc gcaattaaaa gaaaaacagc
      721 tacggctaaa atgcggaaca gccattttgc aacttggctc atctttttgg gtaaatttta
      781 ggttaatgcc atggagatga ttagtgatga tgaagattga aacaaattta gacaaacttt
      841 taaataagcc cgaatatggt tacacaacac gacaaataat tatagaaaag ttcttcctat
      901 caatgtttta acgtaatcaa aagtttaagt ttgctacttg tagctgcgct tcgcatgtag
      961 aaccagagtc aacaggctat cattcttgac gagaacaaag ggtaaaagga aattttagat
     1021 ttttttcagg gtcaaaataa tagatgcatt cgcaattttc tttaagttta gggcaaccat
     1081 taaactcagg cattgcctaa aaatcaaata aaagttaacc attacgatga tgaaaaaaat
     1141 atacctgatt tcttttgctg ccgttgcctg gttatcggcc tgtacaacag gcgagcacaa
     1201 gttggcaacc ggcccctggc tgggggttat ccagctcgat tctacaggcg gcaagctgga
     1261 tgttcccttc aacttcgagc ttaaaaatga ttccggaacc tactccatga
//
LOCUS       DAOOQX010000639         1310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_139814_length_1310_cov_3.099602, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000639 DAOOQX010000000
VERSION     DAOOQX010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139814_length_1310_cov_3.099602"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PLX53_09105"
     CDS             <1..264
                     /locus_tag="PLX53_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOY73280.1"
                     /translation="FLSIDYNDIPDTVKQQGKRTYRSITVGNDELEFSEGFTDLHTTS
                     YRDILSGNGFGLNDARSSIETVYAIRNAVPVGFKGDYHPFCKK"
     gene            288..>1310
                     /locus_tag="PLX53_09110"
     CDS             288..>1310
                     /locus_tag="PLX53_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOY73281.1"
                     /translation="MFKELVSKNEKMAVIGLGYVGLPIALEFAKKISVVGFDIKPDRV
                     ELMKRGVDPSHELESTSFQNCDITFTSDINDLRKASFYVVAVPTPIDEHNLPDLKPLL
                     AATRTVGQVLKKGDYVVYESTVYPGCTEEDCVPILEELSGLKFIQDFKVGFSPERINP
                     GDKNHTLTTIKKITSGCDPESAEEIAKTYELIIKAGIHRASSIKVAEAAKIIENTQRD
                     INIAFMNELSIIFSRMGINTYEVLEAAGTKWNFLNFSPGLVGGHCIGVDPYYLVYKAK
                     ELGYHPQIISAGRYINDTMGGYVAKQTVKKIIAAGTNPMDARVLVMGVTFKENVSDIR
                     NSKVVDI"
BASE COUNT          374 a          283 c          311 g          342 t
ORIGIN      
        1 tttctaagca ttgattacaa cgatattccc gacacagtga agcagcaggg gaaacgaacc
       61 taccggtcca ttaccgtagg gaatgatgag cttgagttta gcgagggttt taccgatttg
      121 cacaccacca gctaccgcga tattctttct ggaaacggat ttggcctgaa tgatgcccgt
      181 agctcaattg aaacggttta tgcgattcgc aacgctgttc cggtcgggtt caagggcgat
      241 tatcatcctt tttgcaagaa gtaatagaat ataacaacat tcactatatg ttcaaggagt
      301 tagtttcaaa gaatgaaaaa atggcggtta taggactagg ttatgtcggc cttccgatcg
      361 ccctggaatt cgccaaaaaa atttcggtag ttggattcga tattaaaccc gacagggttg
      421 agctgatgaa gcgtggagtt gacccaagcc atgagcttga atctacgtcg tttcagaact
      481 gcgacataac atttacttct gatattaacg atttacgaaa ggcgtcattt tacgttgtgg
      541 ccgttcccac acccattgat gagcataacc ttcccgattt gaaaccatta cttgccgcta
      601 cccgaacggt aggccaggtg ctgaaaaaag gtgactacgt ggtttacgag tcaacagttt
      661 atccgggttg caccgaggaa gattgtgtcc ccattcttga ggagctttcc gggttaaaat
      721 ttattcagga ctttaaggtt ggattttcgc ccgagcgaat aaacccgggc gataagaatc
      781 acacacttac aaccataaaa aaaattactt cgggctgcga tcctgaatca gccgaggaaa
      841 tagctaaaac ttatgagttg attattaaag cgggcattca ccgtgccagc tccattaagg
      901 tggccgaagc tgcaaaaatt attgagaaca cccaacgcga tattaacatt gcgtttatga
      961 atgagctctc catcatattc agccgcatgg gtataaacac ctacgaggtg ctcgaagcgg
     1021 caggaaccaa gtggaacttt cttaacttct cacccgggct tgttggaggc cattgcattg
     1081 gagttgatcc atactacctg gtttacaagg ccaaggagct gggctatcat ccgcaaatta
     1141 tcagcgctgg tcgttacatc aacgatacca tggggggata tgtggccaag caaaccgtta
     1201 agaaaattat tgctgccgga acaaatccta tggatgcaag ggtgctggtt atgggtgtta
     1261 ccttcaagga aaatgtaagc gatattcgga actccaaggt agtcgacatc
//
LOCUS       DAOOQX010000640         1307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_140398_length_1307_cov_2.230032, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000640 DAOOQX010000000
VERSION     DAOOQX010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140398_length_1307_cov_2.230032"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLX53_09115"
     CDS             <1..229
                     /locus_tag="PLX53_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY73282.1"
                     /translation="FFAGGVIAVVLVANKIWSQTHMLRYRPVTDQPLFYIALAIAIIG
                     VQLFLAGFIGELVSRSASDRNTYIIEEDTGA"
     gene            341..1087
                     /locus_tag="PLX53_09120"
     CDS             341..1087
                     /locus_tag="PLX53_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOY73283.1"
                     /translation="MKALHSHRSIRRYKSTPIDESLLNEILQAGIRASTTGNMQVYSI
                     IVTKSQEVKQQLLPCHFNQQMVVEAPVVLTFCADFNRFNKWCGQRKAEPGYDNFISFF
                     TGAIDALLVAQNVCIAAEENGLGICYLGTTTYTADRIVEVLKLPKGVVPVTTVVMGYP
                     DESPELTDRLPLAGVVHHEVYQDYSEGVIDQIYMNKESLQLTKKLLEENGKETLAQIF
                     TDIRYTRADNLLFSKVFLKTIEQQGFMNNK"
BASE COUNT          359 a          306 c          308 g          334 t
ORIGIN      
        1 gttttttgcc ggcggggtga tagccgttgt actggtggcc aacaaaatat ggtcgcaaac
       61 ccacatgctg aggtacagac cagtaactga ccagcccctg ttctacattg ccctggctat
      121 agccattata ggagttcaac ttttcctggc tggcttcatc ggcgagttgg tgtcccgcag
      181 cgcctccgac aggaacacct acattattga ggaggatact ggggcataaa aaagatgagc
      241 cgcagcggac tcgtgtaata tgaatgtcca agaacatgaa tttttttgaa ccattttgtt
      301 tcaaaattag ttcctttggt tcattaaaac tcataccaaa atgaaagcac tccattccca
      361 tcgttccata cgcaggtata agagcactcc gattgacgaa tcgctgctta acgaaatcct
      421 tcaggcgggt attcgcgcct caacaactgg caacatgcag gtttacagta ttatagtaac
      481 caaaagccag gaagtaaaac aacagcttct tccctgccac tttaaccagc aaatggttgt
      541 agaggctccg gttgtgctga ccttttgtgc tgatttcaac cgattcaaca aatggtgcgg
      601 gcagagaaag gctgaacccg gatacgataa cttcatctcg tttttcaccg gtgcaataga
      661 cgctttgctg gtggcacaaa atgtatgcat tgccgccgag gagaacggcc ttggtatttg
      721 ctacctgggt accaccacct acaccgccga ccgcatcgtc gaagtactga agctgccaaa
      781 gggagttgtc cccgttacta cggttgtaat gggctacccc gacgagtcgc cggagctaac
      841 cgatcgttta cccttagccg gagtagttca ccatgaggtt taccaggact actcggaggg
      901 cgtcatagac caaatttaca tgaacaagga atcgctgcag ctgactaaaa agctacttga
      961 ggaaaatggt aaggaaaccc tggcccaaat atttaccgat attcgctaca cccgagccga
     1021 caacctccta ttttcgaaag tatttcttaa gaccatcgag cagcaagggt ttatgaacaa
     1081 caagtaggat tgggtttata tgcagctaca cacttttact tcaacacaac tctaggagaa
     1141 aaagcatgaa gacttttaag actattttac tggtgctggt gctgctcctg ataggtgcag
     1201 gggctttcct ctattttgga gtttatgaaa agggtgttat ggccggccga attttaaggg
     1261 tgagccaaaa gggtgttatt tttaagacat acgaggcgaa gctcagc
//
LOCUS       DAOOQX010000641         1306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_140470_length_1306_cov_4.204636, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000641 DAOOQX010000000
VERSION     DAOOQX010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140470_length_1306_cov_4.204636"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            420..>1306
                     /locus_tag="PLX53_09125"
     CDS             420..>1306
                     /locus_tag="PLX53_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY73284.1"
                     /translation="MKAGYRGFLAAAVAVLLVSLVYPSGVFSQDLSQQIVNYEQLAAE
                     FEKNGDANQAAYYFDKAANQAWQARDLNRAIANFDRALANIKQVGNVNGAKTIYTNLG
                     LIYCEKGNHNEAYNNFLAALAAARSLGRKDDIANALVNISTEQIEMSDFTGAQKSLDE
                     AQRLAQEVDNARALKNIYFNYNKLYEKLGSKDKADEYFRLYMMLNKKLQDDEFKARQM
                     LAQAKVDSAGRIINQISYEKESTARKLMETSHELQQTTNILKETERISTEQRMQIDLL
                     NTQMKLREAEIRNQHLLQR"
BASE COUNT          422 a          265 c          282 g          337 t
ORIGIN      
        1 ctaatggcct aaaaatcttc atctactcct aaatcagtat ttttagcata acccccgatt
       61 tccaaccgga agtttttatg attaaaacga gtattgattt tgacccagct gctccgttgg
      121 catttcatat tttacatttt atcccagaaa acttacgatt taagtaaaaa gccgttcatt
      181 ttgtatcgga ataaattgaa aaccgtagaa aatatcaaaa tctagaacgt taaacatttc
      241 gtgttattga tttaataggg gttgcctgaa tagatttata cagaacacct tggcgcaaag
      301 tttttggagt gttttttgcc tccatttaac tcaaggagca ccgtgcaagg taacgtgttg
      361 gccacgtcta taaggcgatt gttttttcgg agggtataaa aaatcaactt ttacgcaaca
      421 tgaaagcagg gtataggggt tttctagcag cagcagtagc agttttactc gttagtttgg
      481 tttatccttc tggcgttttt tcacaggatt taagccagca aatagttaat tacgaacagc
      541 ttgccgctga atttgagaag aatggcgatg ccaaccaggc agcctactac tttgataagg
      601 cagccaacca ggcatggcag gcaagagacc tgaacagggc aattgctaat tttgataggg
      661 cgttggcaaa catcaagcag gtggggaacg tcaacggcgc taaaacaatc tatacaaacc
      721 ttgggctaat atactgcgag aagggcaacc acaacgaggc ctataacaac tttttggcag
      781 cacttgcagc tgccagaagc ttaggccgaa aggacgatat tgccaatgcc ctggttaaca
      841 tttcaacaga gcagattgaa atgtccgatt ttactggtgc acagaagagt ctcgatgaag
      901 cccaaaggtt ggcgcaagaa gttgataatg ccagagcctt aaaaaatatt tacttcaact
      961 ataataagct ttacgaaaaa cttggcagca aggataaggc tgatgagtac ttcaggctct
     1021 acatgatgct aaataagaaa ttgcaggatg acgagttcaa ggcaagacaa atgctcgctc
     1081 aggccaaggt tgacagcgct ggaagaatta ttaatcagat ttcatacgag aaggaatcta
     1141 ctgctcgtaa gctaatggaa accagccatg agcttcagca aaccaccaac atactcaagg
     1201 agaccgagcg aatttccacc gaacagcgta tgcagataga cctgctaaat actcagatga
     1261 agctccgcga agccgaaatt aggaaccaac atctactcca aaggat
//
LOCUS       DAOOQX010000642         1301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_141410_length_1301_cov_3.369181, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000642 DAOOQX010000000
VERSION     DAOOQX010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141410_length_1301_cov_3.369181"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLX53_09130"
     CDS             <1..373
                     /locus_tag="PLX53_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HOY73285.1"
                     /translation="LSVDISRGGLIIPEENGIKQGQEVDLMICWFSEKPLVVGGKYAV
                     KHTTNDLRCLIKEVKYKVNINTLEKDYHEKSIGMNDIACITIKTTKPLFYDSYRQNRI
                     TGSLIIIDEATNVTVGAGMIV"
     gene            complement(374..811)
                     /locus_tag="PLX53_09135"
     CDS             complement(374..811)
                     /locus_tag="PLX53_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOY73286.1"
                     /translation="MYCYEYPRMLVTVDAVIFRTDTQGHPVDLLLIERKNEPYKGCYA
                     LPGGFPEMNELLSDAAARELKEETGLDGITLKQVGAFDRVDRDPRDRNISVAYLGFAS
                     ADSPLPNAGDDAATASWFPLNNLPPLAFDHAEILEKALSNIGI"
     gene            complement(993..>1301)
                     /locus_tag="PLX53_09140"
     CDS             complement(993..>1301)
                     /locus_tag="PLX53_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOY73287.1"
                     /translation="DCSGLVQVVFEIAGTMLPCDARDQAGIGQTIEFANEAKPGDLAF
                     FDDENGVITHVGIVAEDATIIHCSGFVRVDRFDHQGIFNLEAHRYTHKLRIIRRVIFD
                     "
BASE COUNT          354 a          299 c          303 g          345 t
ORIGIN      
        1 gttatcggtt gacatatcgc gtggtggcct catcattccc gaggagaatg gcatcaagca
       61 ggggcaggag gtggatctga tgatttgctg gttttccgaa aaaccactcg tggtgggagg
      121 taagtatgcc gttaagcaca ccaccaatga cctgaggtgt ctgattaagg aggtaaagta
      181 caaggtaaac atcaacaccc tggagaagga ttaccatgaa aagagcattg gaatgaacga
      241 tatagcctgc attacaatta aaaccaccaa gccgcttttc tacgattcat accggcaaaa
      301 caggataacc ggcagcctga taattattga cgaagccaca aacgttactg ttggcgctgg
      361 tatgattgtg tagctaaata ccaatgttgc ttagagcctt ctcaagtatt tcggcatggt
      421 cgaatgccag cggtggaaga ttattaaggg gaaaccagct agcagtagcg gcatcgtctc
      481 cagcatttgg caaagggcta tcagcagagg caaatcctaa gtaggcaaca gaaatgttgc
      541 ggtcgcgggg atctctatcc accctgtcga aagcccctac ctgcttcaac gttattccgt
      601 caagcccagt ttcttccttt aattcacggg ctgctgcgtc cgaaagcagc tcattcatct
      661 ctggaaatcc cccgggcaat gcgtagcaac ctttataggg ttcgtttttc ctttctatga
      721 gcagcaagtc aacagggtgg ccttgagtat cggtcctgaa gatcactgcg tcaactgtta
      781 ccagcattcg gggatattcg tagcagtaca tttttcagct tattacatgg acaactcatc
      841 attattttca tcgtcatctc cctgattgcg ccgcttatcc cttattttaa aggcaatcca
      901 gactgcaata aggatgatga caaagacgat gactatctga tttttcagca tggatttctt
      961 tttggtaaat ataggccttt ggaaaaagat tatcaatcaa aaattactct tcggataatt
     1021 cttagcttat gggtgtagcg gtgggcttcg aggttaaata tgccttggtg atcgaacctg
     1081 tccacacgta caaatcccga acaatgtata atggtggcat cttcggcaac aataccgaca
     1141 tgggtgataa ccccattttc atcgtcgaaa aatgccagat ctccaggttt tgcttcattc
     1201 gcaaattcta tggtttggcc aatgccagcc tgatcgcggg catcacaggg cagcatagtt
     1261 ccggctatct cgaaaaccac ctgcacaagg ccggagcagt c
//
LOCUS       DAOOQX010000643         1301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_141459_length_1301_cov_2.509631, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000643 DAOOQX010000000
VERSION     DAOOQX010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141459_length_1301_cov_2.509631"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PLX53_09145"
     CDS             <1..806
                     /locus_tag="PLX53_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOY73288.1"
                     /translation="SRSVVIAKMASTVRHRESIIMEAIFSMSDIFDTSTVNPSAFTLA
                     SNATIEGGDVLVVRDDVLLIGNGARTNTHGIDFILNRLQLNSDARKKYILVQELPYKP
                     ESFIHLDMVFTLLDADKCMVYDPIIIQPNRYQTVQITIANGRVEEIKTVDNLLVALKK
                     LGMDLKPVYCGGTTDRWVQEREQWHSGANFVAIGPGKVIGYARNVHTIEEMNKNGFEV
                     IRAKDVIAGRVDLGSYSSYVVTIDGSELPRGGGGARCMTMPVRRKKVNW"
     gene            complement(815..>1301)
                     /gene="sucC"
                     /locus_tag="PLX53_09150"
     CDS             complement(815..>1301)
                     /gene="sucC"
                     /locus_tag="PLX53_09150"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539527.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HOY73289.1"
                     /translation="GQRELDEEEPAEIEAGKNNLNFVKLDGNVGCMVNGAGLAMATMD
                     IIKLSGGEPANFLDVGGSANAKTVAAGFRIILKDPNVRVILINIFGGIVRCDRVAQGV
                     VEAYREIGNIPVPVIVRLQGTNALEGRKLIDQSGLRVYTATSFQEAANLVKEMVEKQA
                     F"
BASE COUNT          347 a          293 c          298 g          363 t
ORIGIN      
        1 tatccagaag cgtagtcata gccaaaatgg caagcacggt taggcatcgc gaaagcataa
       61 taatggaggc aatcttttca atgtcggata tttttgatac ctcaacggtt aatccttctg
      121 cttttacact tgcaagcaac gctaccattg agggtggcga tgtgctggtt gttagggatg
      181 atgttttact cattggtaac ggtgcccgaa ccaacactca tggtattgat ttcattttga
      241 ataggttaca attaaacagt gatgctagga aaaagtatat tcttgttcag gagctaccct
      301 ataagcccga gtcctttatt catctcgata tggtttttac tttactggat gcagataagt
      361 gtatggttta cgatccaatt attattcagc ccaaccggta ccaaacagtc caaattacca
      421 ttgcgaatgg aagggttgag gaaataaaaa cggttgacaa cctgttggtt gcgctaaaga
      481 agttgggaat ggacctcaag ccggtttact gcggaggcac caccgacagg tgggttcagg
      541 agcgggagca atggcatagc ggggctaatt ttgtagccat agggcccggc aaggtaattg
      601 gctacgctcg taacgtacac accattgagg agatgaacaa aaacgggttt gaggttatta
      661 gggctaaaga tgtaattgct ggaagggttg atctaggcag ttactccagc tatgtagtaa
      721 ccatcgatgg aagcgaactt ccacgaggtg ggggtggagc caggtgtatg acaatgcctg
      781 tgcggcgcaa aaaagttaac tggtaggatt ccgtttaaaa agcctgcttt tcaaccatct
      841 cctttaccaa attggctgct tcctgaaacg atgttgcagt atataccctt aatcctgact
      901 gatcgataag ctttcggcct tccaaggcat ttgttccttg aagccttact attaccggaa
      961 ctgggatatt gcctatttcc ctgtaggcct ccacaactcc ctgggctacc ctgtcgcaac
     1021 gaactattcc gccaaaaatg tttatcagga taacccttac gttgggatcc ttaagtatga
     1081 ttctaaaccc cgcagcaacg gtttttgcat tagcactgcc acccacatcc agaaaatttg
     1141 ccggttcccc tcctgaaagt ttaataatat ccatagttgc catggccagc ccagccccat
     1201 tcaccatgca gccaacgtta ccatcgagtt taacaaagtt aaggttattc tttcctgctt
     1261 caatttcggc aggttcttcc tcgtcaagtt cgcgttgccc t
//
LOCUS       DAOOQX010000644         1296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_142290_length_1296_cov_3.265109, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000644 DAOOQX010000000
VERSION     DAOOQX010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142290_length_1296_cov_3.265109"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1296)
                     /gene="pheT"
                     /locus_tag="PLX53_09155"
     CDS             complement(<1..>1296)
                     /gene="pheT"
                     /locus_tag="PLX53_09155"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOY73290.1"
                     /translation="LTPNRIDAASHYGVARDLAAYQAFHSNTDTKAVKPDVSGFSIDS
                     KSLTIPVEVVNTSACRRYSAITVSNVTVAPSPEWLQKRLKSIGINPINNIVDVTNYVL
                     HEVGQPLHAFDADAITGGRVVVQTLAEGTPFITLDGVERKLSADDLMICNGSEPMCIA
                     GVFGGLSSGITAKTTRVFIESACFNPVYVRRTARKHGLSTDASFRYERGTDPNITIWA
                     LKRAAMLIREVAGGQISSDIVDVYPEPLDHFRVEFNVDSACRLIGMRIPNDQIERILT
                     SLEISVIEKNDYHWVLAVPTYRVDVTREADVVEEILRIFGYDNVEIPEKVSSTLNFSK
                     KPDNFTMQSIASGFLTGNGFNEIMCNSLTKKDYYEKLTTYPARSSVEILNPLSSDLNV
                     MRQTLLFGGMESIQRNTNYRRPNLKLFEFGNCYRYNADLA"
BASE COUNT          329 a          320 c          302 g          345 t
ORIGIN      
        1 gccaaatcgg cattataccg atagcaattc ccaaattcaa acaacttaag gttgggcctc
       61 ctgtagttgg tgttccgctg aatgctctcc attcccccaa ataggagggt ttgccgcatc
      121 acgttcaagt cgctgctcag cggatttagt atttcaaccg agctacgggc gggataggta
      181 gtcaattttt cgtagtaatc ctttttggtt agtgagttac acataatttc gttgaaacca
      241 tttccggtaa ggaagccgct ggcaatgctc tgcatggtaa agttatcggg ctttttggag
      301 aaattcagag tgctgctgac cttttcggga atttccacat tatcgtaccc gaaaattctg
      361 agaatttcct ccacaacatc ggcctccctg gtaacatcaa ccctataggt aggcacggcc
      421 aacacccagt ggtaatcgtt tttttcgatg actgaaattt caagagatgt cagaatcctt
      481 tcaatctggt cattgggtat cctcatccca ataagccgac aggcactatc aacgttgaac
      541 tctaccctaa aatgatctaa aggttcggga tacacgtcaa ctatatcgga tgaaatttgg
      601 ccaccagcta cctctctgat gagcatggcg gcacgcttta gcgcccaaat ggtaatgttg
      661 gggtctgttc cacgttcgta tcgaaacgag gcatcggtgc taagtccgtg tttgcgggct
      721 gttcgcctta cgtaaaccgg gttaaaacat gcgctctcaa taaacaccct ggtggtttta
      781 gccgtaattc ccgaagacag cccaccaaac acaccggcaa tgcacatggg ttcggagcca
      841 ttgcaaatca tgaggtcgtc ggcagaaagt ttacgttcaa ccccatcgag ggtgatgaat
      901 ggggttccct ctgccagcgt ttgtaccacc acccttcctc cggtaatggc atcggcatcg
      961 aacgcatgca gcggttgtcc cacctcgtgg agcacatagt tggtaacatc aacaatgttg
     1021 ttgatgggat ttatgcctat ggactttagc cttttctgga gccattcggg cgatggcgca
     1081 acagtaacat tacttactgt aatggccgag tagcgacggc aggccgatgt attcaccacc
     1141 tcaacaggga tagtcaaact tttgctatca atgctaaaac cagacacgtc tggcttaaca
     1201 gccttagtat cggtattgct atgaaaagcc tggtatgcag ccaaatctcg ggctacacca
     1261 tagtgcgatg cagcatcaat gcgatttggt gtaagc
//
LOCUS       DAOOQX010000645         1293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_142852_length_1293_cov_3.697092, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000645 DAOOQX010000000
VERSION     DAOOQX010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142852_length_1293_cov_3.697092"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..>1293
                     /locus_tag="PLX53_09160"
     CDS             157..>1293
                     /locus_tag="PLX53_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY73291.1"
                     /translation="MSVGYSEESIKTLEWQDHIRKRPGMYIGKLGNGSQPDDGIYVLL
                     KEVVDNSVDEFMMGAGKRIEISVVGHTVSVRDYGRGIPQGKLMDVASKMNTGAKYDSK
                     AFKKSVGLNGVGIKAVNALSKKFIIRSIREGIAKEIEFSAGLPVREQNYASNGELTGT
                     FIEFLPDDAVFGEYAFQDEYIEQMIRNYTYLNTNLTITFNGKEFVSKNGLLDLLNENL
                     GGEPLYPIIHLTGNDIEVAITHSSGYGEDYYTFVNGQHTIQGGTHLTAFRESYVKTIR
                     EFYKKDFDPADVRMSLIAAISIKVEEPIFESQTKTKLGSKDMSPDGPSIRQFIGDFIK
                     KELDNYLHKHSETASILLQKILESEKERKAISGIKKIARERAKKA"
BASE COUNT          380 a          299 c          303 g          311 t
ORIGIN      
        1 cctttatcct tgtattaccc tttatccttg tcttgccttt tcccagcaga gcgtaaaagt
       61 tcaaaaaaaa gccttacttg ttgatattta ttgccagcag cagtggctag catatttgga
      121 ataataccta cttttgctgc aaattcaaac gtagctatga gcgtaggtta ctcggaggag
      181 agcattaaaa cacttgaatg gcaggatcat attcgcaagc ggccggggat gtacatcggc
      241 aagctgggca atggctctca gcccgatgat ggtatttacg ttctacttaa agaggtagtt
      301 gacaactccg ttgatgagtt catgatgggc gccggtaaac gaattgaaat atccgtagtt
      361 ggtcataccg tttctgttcg agactacgga aggggcatcc cgcagggaaa actcatggat
      421 gtggcctcta aaatgaatac cggagctaag tacgactcta aggcatttaa aaaatcggtt
      481 gggcttaacg gtgtaggaat aaaggctgta aatgccctat cgaaaaagtt catcatacgt
      541 agcattcgcg agggcattgc caaggaaata gagttcagcg cagggctccc cgttagagag
      601 caaaactacg cctccaatgg agagcttacc ggaacattca tcgagttttt acccgatgat
      661 gccgttttcg gcgaatacgc ctttcaggat gagtacattg agcagatgat ccgtaactac
      721 acctacctga acaccaacct taccatcacc ttcaacggca aggagtttgt atcgaaaaac
      781 gggctgctcg acctgcttaa cgaaaacctt ggcggcgaac cgctctaccc cattatccac
      841 cttaccggta acgatattga ggtggccatt acgcacagta gtggttatgg tgaagactat
      901 tacacttttg ttaacggaca gcacaccatt cagggaggca cccacctaac cgcattcagg
      961 gaatcctacg ttaaaaccat tcgggaattc tacaaaaagg acttcgatcc ggccgatgtt
     1021 cgcatgtcgc tcattgcggc tatcagcata aaggtggaag agcctatttt tgaatcgcaa
     1081 accaaaacaa agctgggaag caaggatatg agccccgacg ggccatccat taggcagttc
     1141 attggtgatt ttattaaaaa ggagctggac aactaccttc acaagcacag cgaaaccgcc
     1201 agcatcctgc tccaaaagat tcttgagtcg gaaaaggagc gtaaggctat ctccggaata
     1261 aaaaaaattg cccgtgaacg ggcaaaaaag gcc
//
LOCUS       DAOOQX010000646         1293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_142888_length_1293_cov_2.889338, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000646 DAOOQX010000000
VERSION     DAOOQX010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142888_length_1293_cov_2.889338"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            701..>1293
                     /locus_tag="PLX53_09165"
     CDS             701..>1293
                     /locus_tag="PLX53_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73292.1"
                     /translation="MAKRLTRREFIRNGLLAAGGLYLTRFTSLRATAAGNQKPWKWSR
                     EAMFYTLTPRGARCEICPNECTIRPGESGDCRNRENTNGKLYSIAYGNPCSVHVDPIE
                     KKPLYHFLPGSQSFSIATAGCNFACMNCQNWEISQTSPRKTRNIELFPEQVVEQCLLS
                     GSKTIAYTYSEPTSLYEYMFDTAVIAQLPSGKVYQFG"
BASE COUNT          366 a          300 c          292 g          335 t
ORIGIN      
        1 gtaagctgtg aaaacatgca aatttcaatt ctcgaaaaat agttgtttct tatcaaaaat
       61 cacaccagat aagcctattt ttttacataa attaggtttg aatattttct gaaacaccca
      121 tgtaaggcag cttgcacaaa cagggatgaa ccgatccgcc agctggcgga gagctcggcg
      181 aagccaataa aatattggtg ttccatccga acgttaacga gtgcttaagg cgaaatcgta
      241 agttattgtc aattaataat attagatttt atacggatga aaacaatgaa caaagacata
      301 actaagccca tataccataa gggtttcatt gataatttgt acgttctgtc aaatctaaat
      361 taggtgcatt tgggaagaga ggtaaccacc caataattta ctgtatatgg cctcagccat
      421 tatgagaaag cgcataacag accctcttgc tcccacacga cggaagtcca gcggcagcaa
      481 gggagtttac ctgacgaatt ttggaagcta cgcctattat cggtaccgac cagcttctct
      541 gtatatggtt ctttgctgcc gttgtctggc aggggttacc cccccaaatt tgcttaaatg
      601 tatggtaatg gttaacttta ttgcccacct caggtagggc taatgatacc gttatgaggt
      661 aagttttggg taacattttg tgtacgtata gctaggtagc gtggctaaaa ggttaacacg
      721 acgtgagttt atcaggaacg ggctgctggc agcgggaggt ttatatctta cccgttttac
      781 aagtctaagg gcaacggctg ccggcaacca aaagccatgg aaatggagca gggaggctat
      841 gttttacacg ctaaccccac ggggtgcgag gtgtgaaata tgccctaacg agtgcactat
      901 tcgtcccggg gaatcgggcg actgtcgtaa cagggagaac accaacggca agctttactc
      961 cattgcctat ggcaacccct gctcggttca tgtggaccca atcgaaaaaa agcctcttta
     1021 ccactttttg cccggttcgc agtccttttc aatcgctact gccggatgca actttgcctg
     1081 tatgaactgc cagaactggg aaatttcaca aaccagccct cgaaaaaccc ggaatattga
     1141 actcttcccc gagcaggtgg tggagcaatg cctgctatcc ggatcgaaga ccattgccta
     1201 cacttactcc gagcccacct cactttacga gtacatgttc gacaccgctg taatcgccca
     1261 gctcccatcc ggaaaagtgt atcagttcgg caa
//
LOCUS       DAOOQX010000647         1293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_142901_length_1293_cov_2.659935, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000647 DAOOQX010000000
VERSION     DAOOQX010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142901_length_1293_cov_2.659935"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1155
                     /locus_tag="PLX53_09170"
     CDS             82..1155
                     /locus_tag="PLX53_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73293.1"
                     /translation="MKKLVKLMMAMLVLAFAFASCGGDDETQYAEFENGAIILNEGAY
                     GNSNASITFIGYGSDIADNQIFYRINGRELGDVLQHAAIYNGKIYMVLNNSGKVEVAS
                     SATFIQLQTITGLVNPRYVAAKDGKIYVTQWGSWGEQGTVEVFDANSYSHTNTIKVGT
                     GAEGLLCTDGKIWVANGGGYGLDSTVMVVDAATGAVSDTVTVGYNPKELVEDASGNIW
                     VICSGADIYDDSWEVVGHLPSKLVMIDRQSRQVVHSLIISNTSHPAHIDVSSGKETVY
                     FGGGFGVNGIFSVSITQPVIPQTPVINGFFYGFNIRPSNGDIYCMVAPNFTSAGELKI
                     YSSAGAALKSYSVGIAPSTALFN"
BASE COUNT          353 a          256 c          316 g          368 t
ORIGIN      
        1 ccatgccact taggagttat atgttcacca taaatattga ctttaccagg taaattgatt
       61 cattaactta aacctttaac catgaagaaa ttagtaaaat taatgatggc gatgcttgtg
      121 cttgcctttg cctttgcctc ttgcgggggt gatgatgaaa ctcagtatgc tgagtttgaa
      181 aacggtgcaa ttattctgaa cgagggagct tacggtaact ccaatgcttc gattaccttc
      241 attggttacg gttcagatat tgccgataac caaatatttt accgcataaa tggccgtgag
      301 ctgggcgatg tgcttcagca tgccgctatc tataatggaa agatttacat ggttttgaac
      361 aactctggca aggtggaggt ggccagcagc gctacattca ttcaacttca aacaattact
      421 ggactggtaa acccacgtta tgtggctgca aaagatggaa aaatttatgt tacccaatgg
      481 ggtagttggg gcgagcaggg cactgtcgag gtgttcgatg cgaatagcta ttcacatact
      541 aatactatca aggttggaac aggcgcagag ggtttacttt gtaccgatgg gaaaatttgg
      601 gtcgccaatg gaggagggta tggcttagac tccacggtta tggtggttga tgctgcaacc
      661 ggcgctgttt cggacaccgt aactgttggc tacaatccca aagagctggt tgaggatgcc
      721 agcgggaata tctgggttat ttgttccggt gccgatatct acgatgacag ctgggaagtt
      781 gtaggtcatt tgccttccaa gctggtaatg atagaccgtc agagccggca agtagttcat
      841 agcctgatta tttccaatac cagccaccct gctcatatcg atgtaagttc gggaaaagaa
      901 accgtgtact ttggcggagg attcggggta aatggaattt tctctgtaag cattactcaa
      961 cccgtgattc cccaaacacc tgttattaat ggtttctttt atgggttcaa cattaggccc
     1021 agcaatggtg atatctactg catggttgca ccaaatttta cctcggcggg tgagctgaaa
     1081 atatatagct ctgctggcgc tgccctaaaa agttatagtg taggaattgc acccagcact
     1141 gccttgttca actaatgaaa tatcaggtat tatgcaaaaa aacctgtcga aagtacatga
     1201 aacataagcg acaggttttt ttaagcatta ctgtgattta ggccttctta ggggtgaagg
     1261 cacaacatgg gcatccataa attcgctgtc cag
//
LOCUS       DAOOQX010000648         1293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_142920_length_1293_cov_2.512924, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000648 DAOOQX010000000
VERSION     DAOOQX010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142920_length_1293_cov_2.512924"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..>1293
                     /gene="ygeW"
                     /locus_tag="PLX53_09175"
     CDS             253..>1293
                     /gene="ygeW"
                     /locus_tag="PLX53_09175"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF005538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOY73294.1"
                     /translation="MDRVEIIERLKTNLHGRDFLLTWEKSEDELRMILGVADILKEMR
                     NRNISTRVFDSGLAISNFRDKSTRTRFSFASASNMLGLAVQDLDEEKSQISHGETVRE
                     TANMISFLSDFIGIRDDMYLGEGNKYMRQVGTALDDGFANGVLPVRPGIVNLQCDMDH
                     PTQVMADLLHLTHEFGSLEALRGKKLVMSWAYSPSYGKPLSVPQGVIALMTRFGLNVE
                     LAYPEGYDLIPEIVDVARNNAAKSGGSFTIKHSMAEAMRGADIVYPKSWAPFHIMEQR
                     TQLLKSGDKKLLNELEQTCLANNARHKDWEYNEEMRRLTSKSDALYLHCLPADISGES
                     CAQGEVSAETFER"
BASE COUNT          332 a          288 c          331 g          342 t
ORIGIN      
        1 cgggcaacga ggtgctggct catgctccaa acgaaaaggt gcctgttaac gatttggtgg
       61 ttgcctcagc attttacacc ctatttgcct acaccatcta gctggttgaa atttttaggt
      121 ttactttctc tttaccattt tagggcagtg atgctgctgt tatttttgtt tagaggaatt
      181 ttaatggaag ctgcctatag ctattgctaa tcgagttaaa aagttgtctg tttatttaaa
      241 caatttatgt tgatggatag agttgaaatc atcgaaaggt taaaaaccaa cctgcatggc
      301 agggacttcc tcctaacgtg ggagaaaagt gaagacgagt taaggatgat acttggggta
      361 gccgatatcc ttaaggagat gagaaaccgt aatatatcta caagggtatt cgactcgggc
      421 ctggcaattt ccaattttag ggacaagtca acccgtaccc gattttcttt tgcatcggcc
      481 tccaacatgc tggggctggc tgttcaggat cttgacgagg agaaatcgca gatttcgcat
      541 ggggaaaccg ttcgtgaaac tgccaacatg atttcgtttc tatccgattt tataggcatt
      601 cgcgacgata tgtaccttgg cgaaggtaac aagtacatgc ggcaggttgg cacagcactc
      661 gacgatgggt ttgccaacgg cgtgctaccg gtgaggccgg gtattgtcaa cctgcaatgc
      721 gatatggacc acccaaccca ggttatggcc gatttgcttc accttaccca tgaattcggc
      781 tccctggaag ccttgagggg taaaaagctg gtgatgagtt gggcttactc ccctagctac
      841 ggaaaacctc tatcggtccc ccagggggtt attgccctta tgacccgttt cggcttgaat
      901 gtggagctcg catacccgga aggttacgac ttaatacccg agattgttga tgtagctagg
      961 aataatgctg ccaaaagcgg cggatcgttt accattaaac attccatggc cgaagccatg
     1021 cgcggcgctg atatagttta tccaaaaagc tgggctccat ttcacatcat ggagcagcga
     1081 acccaattgc taaaaagtgg cgataagaag ttactgaacg agctggagca aacctgcctg
     1141 gctaacaatg cccggcataa ggactgggag tataacgagg agatgcgtag gttgaccagt
     1201 aagtccgacg ccctttacct ccactgcctg cccgccgaca tttccggtga atcgtgtgca
     1261 caaggtgagg taagtgctga aacttttgag cgc
//
LOCUS       DAOOQX010000649         1291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_143323_length_1291_cov_2.266990, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000649 DAOOQX010000000
VERSION     DAOOQX010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143323_length_1291_cov_2.266990"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PLX53_09180"
     CDS             complement(<1..585)
                     /locus_tag="PLX53_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOY73295.1"
                     /translation="MVKFILQQSLNPYFNIASEEYLLRSFSDDFIYIYRCNPSIVVGK
                     HQNTFAEVNIRLVERLSIPVVRRLSGGGTVFHDLGNINFCFISNGVPGQLVDFKRFTL
                     PVIDFLRSLGVNAYLGQKHDIRVGDRKISGNASYAFGNRVMHHGTLLFSSDLEFLAEA
                     IRVEPGLFTGSAIQSNRSRVANTVDFLSPKMDIEQ"
     gene            658..1065
                     /locus_tag="PLX53_09185"
     CDS             658..1065
                     /locus_tag="PLX53_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HOY73296.1"
                     /translation="MTTYESKIVAISKRAEDIYGMLSDFRNFTPMVAQRVEEWQAEEQ
                     RCSFKYQGKGPVGLKIVEKEKNKTIKITGDDNLPMQFYLWIQLKEVAPYDTRMKITLK
                     AELNMMMKMLLGKKLDEGVNAMAEGIAAAFNTI"
     gene            complement(1087..>1291)
                     /locus_tag="PLX53_09190"
     CDS             complement(1087..>1291)
                     /locus_tag="PLX53_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY73297.1"
                     /translation="SSELKGKLFKADRDATTPGLHNEKGSGLGLILCKTFVEQMGGNI
                     LVESQPGLGSTFTVTLKLEGIPG"
BASE COUNT          382 a          295 c          292 g          322 t
ORIGIN      
        1 ctgctcgata tccattttcg gcgaaagaaa atcaaccgta ttggcaactc tgcttctgtt
       61 cgattgaatg gcactaccgg taaaaagccc cggttcaacc ctaatagctt cggcaagaaa
      121 ctccaaatcg gatgaaaaca ggagagttcc gtgatgcatt acccgattac caaaagcata
      181 gcttgcattg cccgatattt tcctatcgcc cacccgaatg tcgtgcttct ggcccaaata
      241 ggcattaacc ccgaggctac gaagaaaatc gataaccggt aaggtaaaac gtttaaaatc
      301 taccagctgc ccagggaccc cattcgaaat aaagcaaaag ttaatgttgc ctaaatcgtg
      361 aaacacggtt cctccacccg atagccttct aaccacagga atggagagcc gctccaccaa
      421 ccgaatgttg acctcggcaa acgtattttg gtgcttacca acaactatcg aggggttgca
      481 tcggtagatg tagataaaat cgtcgctaaa gctgcgcagc aggtattcct ccgaggcaat
      541 gttaaagtag ggatttaggc tttgctgaag aataaactta accatggagt aagcaccggg
      601 ggctaaccgg aatacttaaa ttttcactta atttgcatcg acaaatttag gcaaaaaatg
      661 accacatacg aaagtaaaat tgttgccatc tccaagcgcg ctgaggacat ttacggaatg
      721 ctttcggact ttagaaattt tactccaatg gttgcccaaa gggttgagga atggcaggcc
      781 gaggagcaaa gatgtagctt taaataccag ggaaaagggc ctgtagggct aaagattgtg
      841 gaaaaggaaa aaaataaaac catcaagatt accggtgatg ataacctccc catgcaattt
      901 tatctttgga ttcagctaaa ggaggttgct ccctacgata cccgaatgaa aattacccta
      961 aaggctgaac tcaacatgat gatgaaaatg ttgctgggta agaagctcga tgagggcgtt
     1021 aatgccatgg ccgagggaat tgcggcagca ttcaatacca tttaaatggg tagtattgag
     1081 gagcggctag ccaggtattc cctctagttt taatgtaacg gtaaacgtgc ttcctagtcc
     1141 cggttggctc tccaccagga tgtttccgcc catttgttct acaaacgttt tgcagagtat
     1201 cagcccaagc cccgaaccct tttcgttgtg gagacccgga gttgttgcat ctctatcggc
     1261 tttgaatagc ttccctttaa gctcgctgct t
//
LOCUS       DAOOQX010000650         1289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_143599_length_1289_cov_3.047812, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000650 DAOOQX010000000
VERSION     DAOOQX010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143599_length_1289_cov_3.047812"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..>1289)
                     /locus_tag="PLX53_09195"
     CDS             complement(7..>1289)
                     /locus_tag="PLX53_09195"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HOY73298.1"
                     /translation="KPLGAQFYPPDMTIEEFEAFSSPDKTSQYTLIRRNSDGKLESVP
                     YHITYKDRIDKAAELMVQAANLAEDAGLKRYLNLRAKALQTDEYFESDMAWMNMKNNN
                     IDFVVGPIENYEDALFNYKAAYEAYILIKDLEWSQKISHYAALLPKLQESLPVDPAYK
                     KEVPGLDSDLGVYDVVFYAGDCNAGSKTIAINLPNDPKVQLKKGSRKLQLKNAMRYKF
                     DKILLPIGNLLIDEEQREHIKFDAFFENVMFHEVAHGLGIKNRVDGKGGVQESLKEMH
                     SALEEGKADILGLYMVSQLAAMGELPDKDMMDNYVTFVAGIFRSVRFGAASSHGKANM
                     VCFYYLKERGAFERNPETGTYRVNFDKMTEAMNSLAHDILILQGNGDYEGVKKLFADK
                     GFIAEELQHDLDWVSEASIPRDIRCIQGKEIFGL"
BASE COUNT          312 a          325 c          280 g          372 t
ORIGIN      
        1 tacaagctat aagccaaata tttctttgcc ttgaatacat ctaatgtctc gtggaatgct
       61 agcctccgaa acccaatcca ggtcatgctg tagctcctcg gctataaatc ccttatcggc
      121 aaaaagtttt ttaacaccct cataatcccc gttcccctga agtattagga tatcgtgggc
      181 aagggagttc attgcttcgg tcattttatc gaagttaacc cggtaggttc cggtttcggg
      241 atttcgctca aaggcaccac gctccttgag gtagtaaaag caaaccatgt tcgcttttcc
      301 atgcgaagag gcagctccaa aacgtacaga tcggaaaata ccggcaacaa aggttacata
      361 gttgtccatc atgtccttat ccgggagttc tcccatggca gccagctgcg acaccatgta
      421 aagaccaagt atatcggcct tcccctcttc cagtgcgctg tgcatctcct taagcgattc
      481 ctgcacacca cctttcccat caacccggtt tttgattccc aagccgtggg caacctcatg
      541 aaacattacg ttctcaaaaa aagcatcaaa tttgatatgc tcgcgttgct cctcgtctat
      601 cagcaagttg ccaatgggca ggagaatctt atcgaacttg tacctcatgg catttttcag
      661 ctgcagcttt cggctaccct tcttgagctg aacttttgga tcgtttggta ggttaatggc
      721 aattgttttg cttcctgcat tgcagtcgcc tgcatagaac acaacatcgt acacgcccaa
      781 atcggaatca agcccaggaa cttccttctt atatgcagga tcaaccggca ggctctcctg
      841 cagcttggga agcagagcgg cgtaatggct aattttttgt gaccattcaa gatccttaat
      901 caggatgtag gcttcgtagg cggccttgta gttgaacagt gcgtcctcgt agttttcaat
      961 tggcccaaca acaaaatcta tgttgttatt tttcatgttc atccaggcca tatcgctttc
     1021 aaagtactcg tcggtttgca gcgctttagc tctaagattt aaatatcgtt tcaagcctgc
     1081 atcctcggct aagttggctg cctgcaccat tagctcagcc gccttgtcta tcctatcctt
     1141 ataagtaata tggtagggaa ccgattcaag cttcccatca gagttgcgcc tgatgagggt
     1201 gtactggctg gttttgtcgg gagagctaaa ggcttcgaac tcctcaatgg tcatgtcggg
     1261 aggataaaat tgggcgccta ggggttttt
//
LOCUS       DAOOQX010000651         1285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_144348_length_1285_cov_2.868293, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000651 DAOOQX010000000
VERSION     DAOOQX010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144348_length_1285_cov_2.868293"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /gene="lpxC"
                     /locus_tag="PLX53_09200"
     CDS             complement(<1..706)
                     /gene="lpxC"
                     /locus_tag="PLX53_09200"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOY73299.1"
                     /translation="MADKQRTISQPVTLTGKGLHTGVDVEVTICPAEVNTGVNFQRTD
                     LEGQPKIEAVADNVIDTSRGTSIGTKNVKVNTIEHMVAALYGMGIDNALVKVNGPEMP
                     IMDGSSRFFVEAIASVGTVEQGAIRNYFTIKEKMVFSDEKNGIEIVAYPDDGFSIDLM
                     VDYNSRVLGHQYAKMGNIDEFASQIAPCRTFVFFHELEVLLKHNLIKGGDLENAIVII
                     EHEVPQPELDRIADLFN"
     gene            complement(815..>1285)
                     /locus_tag="PLX53_09205"
     CDS             complement(815..>1285)
                     /locus_tag="PLX53_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOY73300.1"
                     /translation="ANYKKIPQIGNVVLEDYVEIGANATVDRATMGSTILHRGVKIDD
                     HVHIAHNVEVGENTVMAAQCGVAGSTKIGKNCMFGGQVGASPHIKIADGVMAGAQSGI
                     AGDIRKPGTIILGSPAFDVSKQRRSMAVYKNLPDMANKLNEMQRTIEELKKKLL"
BASE COUNT          326 a          310 c          268 g          381 t
ORIGIN      
        1 tgttgaagag gtcggcaatt ctgtcgagct cgggctgcgg aacctcatgc tctataatga
       61 caatggcatt ttccaggtca ccacccttaa taaggttatg ctttagcaac acctcgagct
      121 cgtgaaagaa tacgaaggtt ctgcaaggtg ctatttggct ggcaaactcg tcaatattcc
      181 ccattttggc gtactgatga cccagcaccc ttgagttgta gtcaaccatc aggtcgatgc
      241 tgaatccgtc gtcgggatag gcaactattt cgataccatt tttttcatcg gagaaaacca
      301 ttttctcctt tatggtaaag tagttacgaa tggcaccctg ttcaactgtt ccaacactag
      361 caatagcctc aacaaagaac ctggagctac catccataat tggcatttcg gggccattca
      421 cttttaccag ggcattatct attcccatgc cgtaaagagc ggcaaccatg tgctctatgg
      481 tgttgacttt tacatttttg gtgcctatgg atgttcccct ggaggtgtca ataacgttat
      541 cggcaaccgc ttcaatcttg ggttgtcctt ccaaatcggt tcgttgaaag ttaacaccgg
      601 tgttcacctc tgccggacaa atagtaacct caacatcaac accggtatgc aacccctttc
      661 cggtaagggt aactggctgg cttattgtgc gttgtttatc tgccataaaa aggttttcaa
      721 attaaacata ttccctggtt ccaggggttc tacatattca aggtttcttt ttaataagtc
      781 aaaaaatata aagagaaacc ggctatagca ataattataa taattttttc ttcaactcct
      841 caatggtgcg ctgcatttcg tttagcttat tggccatgtc aggcaaattc ttataaacag
      901 ccatagaccg gcgttgtttg gagacatcaa atgccgggct tcccaaaata attgtaccgg
      961 gtttccgaat gtcgccggca ataccggact gtgcgccggc catcacacca tcggctattt
     1021 tgatgtgggg gctggctcct acctggccac caaacatgca gtttttccca attttagtcg
     1081 atccggccac tccgcattgg gctgccataa cagtattttc accaacctcc acgttgtggg
     1141 caatatgaac atggtcgtca attttaacgc ccctatggag aatggtagaa cccattgttg
     1201 cccggtcaac agtagcattt gcaccaattt ctacataatc ctcaaggact acgttgccaa
     1261 tttgcggtat ttttttgtag ttagc
//
LOCUS       DAOOQX010000652         1276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_145990_length_1276_cov_3.131859, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000652 DAOOQX010000000
VERSION     DAOOQX010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145990_length_1276_cov_3.131859"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..>1276)
                     /locus_tag="PLX53_09210"
     CDS             complement(131..>1276)
                     /locus_tag="PLX53_09210"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOY73301.1"
                     /translation="RRQVQSSGSGVILTNDGFIVTNNHVVENAEDITVVLNDKHKFSA
                     KVVGADPNTDIALLKIDAKDLPTIPFGNSDDIKVGEWVLAIGNPFNLNSTVTAGIVSA
                     KARNIQILSNREFAIESFIQTDAAVNPGNSGGALVNLRGELIGINTAIASRTGTFAGY
                     AFAVPSSIVKKVVTDLIEFGEVRRAVMGVRIQELTDELAKEKGIKDIKGVYVADVEKN
                     GAADKAGIKAGDVILSINNVDVNSTAQLQEQISRYRPDQQVDVLVNRDNTKKHYTVTL
                     SNLKSEMKFSKSEDAFSSLGATFAEVPAKLKRDLGIDYGLQVVELADGKLKDGGIRQG
                     FIITRVNRTPIRSEDDLKRVLSISSGGVLIEGVYPNGTVGYYAIGVD"
BASE COUNT          337 a          329 c          259 g          351 t
ORIGIN      
        1 tcccaagccg gcgtgtgcaa gaacttgcat cttgccggta gtgtagtgta gtagtgtttg
       61 cccgacacac cataccaata aggccgcttg ataagcggcc ttatataacc tataagatat
      121 tttcctggtt ctaatctaca ccaattgcgt agtaaccaac agttccgttg gggtaaacac
      181 cctcaatcag cacacccccg gacgaaatgc taagcacccg cttcaaatca tcttcactcc
      241 ttatcggtgt tcggttaaca cgggtaatta taaatccttg cctaatgccc ccatccttaa
      301 gcttgccatc ggctagctca acaacctgaa ggccatagtc tatgcccaaa tcccttttca
      361 gcttggctgg tacctcggca aaggttgcac caagactgct gaaggcatct tcagactttg
      421 aaaacttcat ctcgcttttc agattgctaa gggtaacggt gtaatgtttc ttagtattat
      481 cacggtttac cagaacatcc acctgttggt caggccggta gcggctaatt tgctcctgaa
      541 gctgggcagt gctgtttaca tcaacattgt tgattgaaag aataacatcg cccgctttga
      601 tacctgcttt atcggcagca ccattctttt caacatcggc aacatacact ccttttatat
      661 ccttaatgcc tttttcctta gccagctcat cggttagctc ctggattcga acacccataa
      721 cagcacgccg cacctcacca aactctatta agtcggtaac caccttttta actattgatg
      781 agggaacagc aaaggcatac cctgcaaatg tgccagtacg ggaagcaatg gcggtattaa
      841 tgccaatgag ctcacccctt aggttaacca gcgcaccacc actattgccc gggttaacag
      901 ctgcgtcggt ttgaatgaac gattcaatgg caaactccct gttgctaagt atttgaatgt
      961 tacgagcttt agcacttaca atacctgcag taaccgttga gttaaggttg aaagggttgc
     1021 caattgccaa aacccactcc cctaccttaa tgtcgtcgga attaccaaaa ggaatggtgg
     1081 gaaggtcctt ggcatcaatt ttaagaaggg caatgtcggt gttggggtcg gctccaacaa
     1141 cctttgcaga aaatttatgc ttatcgttta aaactacggt aatatcctcg gcattttcca
     1201 ccacatgatt gttggttacg ataaaaccgt cgtttgtaag aattacaccc gacccggaac
     1261 tctgcacttg acgtcg
//
LOCUS       DAOOQX010000653         1276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_146073_length_1276_cov_2.041769, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000653 DAOOQX010000000
VERSION     DAOOQX010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146073_length_1276_cov_2.041769"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /gene="pepT"
                     /locus_tag="PLX53_09215"
     CDS             <1..1182
                     /gene="pepT"
                     /locus_tag="PLX53_09215"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOY73302.1"
                     /translation="QSDDTVSDRFPSTEKQLVLSNLLVKELKELGVSDATIDEFGYVM
                     GTIPANSTKKVPVIGFLAHVDTSPDMPGKNVKPRFVESYDGSDITLNAEKGVILSARE
                     FPELKEYTGQTLIVTDGTTLLGADDKAGIAEIMTAVEYITGNPTLRHGKIRIGFTVDE
                     EVGRGVDHFDVKRFGADYAYTLDGGAVGELEFENFNAASAKITIQGRNIHPGYAKDKM
                     LNAILIAQEFNSMLPPHESPEHTEGYDGFYHITKIEGTVEKASFQYIIRDHDMRKFER
                     RKAYMERVARYLNSKYGDQTVTLEINDQYYNMREKVEPVYHVVQTAIEAMEKVGIKPK
                     VRPIRGGTDGARLSYMGLPCPNLFAGGENFHGKHEYVPVESMVKATETILKIVEIYES
                     K"
BASE COUNT          360 a          303 c          315 g          298 t
ORIGIN      
        1 caaagcgacg atacggtatc cgatcggttt ccaagcactg aaaaacagct ggttctatca
       61 aatttgctgg tgaaggagtt aaaggagctt ggcgtatccg atgcgacaat cgacgagttt
      121 ggctacgtta tgggcaccat tcctgccaat tcaacaaaga aagttcctgt tataggcttt
      181 ctggcacatg ttgatacctc gcccgatatg cccgggaaaa atgtcaagcc acgctttgtt
      241 gagagctacg atggctccga cattaccctg aatgcagaaa agggggttat tctatcggcc
      301 agggagttcc ccgagctcaa ggagtatact gggcaaacac ttatagttac cgatggcaca
      361 accctgcttg gagccgacga taaggccgga atagctgaaa tcatgacggc cgttgagtac
      421 atcaccggca atccaacttt acgacatggt aaaattcgta ttggcttcac ggttgacgag
      481 gaggttggtc ggggggttga ccacttcgat gtgaaacgtt tcggcgccga ctatgcctat
      541 acgcttgatg gtggtgctgt tggagagctg gagtttgaga acttcaacgc tgccagtgcc
      601 aagattacca ttcagggccg gaatatacat ccgggctatg ctaaggataa aatgcttaac
      661 gccattctta ttgcccagga gttcaacagc atgttaccgc cccacgagag ccccgagcac
      721 accgagggct acgatggttt ctaccatatc accaaaattg agggaaccgt tgagaaagct
      781 tcatttcagt acatcatccg cgatcatgac atgaggaagt ttgaaagacg caaggcatac
      841 atggagcgtg ttgcccggta ccttaacagt aagtacggcg accaaacggt tacccttgag
      901 attaacgacc agtactacaa catgcgcgaa aaggtggaac ctgtttacca cgtggtgcag
      961 accgccattg aggcaatgga aaaagttggt attaagccta aggttcgacc catacgcggt
     1021 ggaaccgatg gtgccaggct ttcctacatg ggactccctt gcccaaacct gtttgcaggc
     1081 ggtgaaaact ttcatggaaa acacgagtat gtcccggttg aaagcatggt gaaggccacc
     1141 gagaccatac tgaaaattgt tgaaatttac gagtcaaaat agatatttaa gtttcaaccc
     1201 gcatacactt caaccgtaaa caacatttat cctgtaagca gaatgccgct cataaacttt
     1261 catacccacc tcacaa
//
LOCUS       DAOOQX010000654         1275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_146191_length_1275_cov_2.740164, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000654 DAOOQX010000000
VERSION     DAOOQX010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146191_length_1275_cov_2.740164"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLX53_09220"
     CDS             complement(<1..217)
                     /locus_tag="PLX53_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73303.1"
                     /translation="MKIARVIVVSLALIMAVTTARAQDYKTGLGLRGGYPSGITVKHF
                     FGSKTAVDGILSFGWGGILLESGVKVHK"
     gene            complement(300..>1275)
                     /locus_tag="PLX53_09225"
     CDS             complement(300..>1275)
                     /locus_tag="PLX53_09225"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOY73304.1"
                     /translation="DFDYMVGAQLEGFDNMVGLNNQTNLAVFEGDEYLTSPVDPRPKF
                     HLYKPTIAVISGIAWDHFNVFPTIENYRDQFVGFISCIEPNGTLVYCSEDDEVAGVVS
                     STKRSDLELIPYTTHPYRIEPNVGCQLVTEQACYPLKIFGRHNMQNISAAKAVCLKLG
                     VSESNFYRAISTFKGAAKRLQRLAEGANSTVFLDFAHSPSKVAATIGAVLEEYPNRKL
                     VACLELHTFSSLNINFMSQYRNVFSGVAVPIVYFDPVTVEHKNLPPLNINEVRDAFGQ
                     PIPDVCISASEMVEKLNGMNWENANLLLMTSGNFSGLNLNGLALQIAR"
BASE COUNT          350 a          294 c          272 g          359 t
ORIGIN      
        1 atttatgaac tttaactcct gattcaagta aaattccacc ccagccaaac gaaaggatgc
       61 cgtcaacagc tgttttactg ccaaaaaaat gtttaaccgt gatacccgaa gggtatccgc
      121 ctcgtaatcc caaaccggtt ttgtagtcct gcgctcttgc ggttgttacc gccattataa
      181 gcgctagcga taccactatt actcttgcta ttttcatgtg cttttatttt ggttagttaa
      241 tatgtgttgt aaaagtagga acaatatcct ataacaggat atctttaaca aagtttaaat
      301 catcgtgcta tttgtaaagc caacccgtta aggtttaaac ccgaaaagtt ccccgaagtc
      361 atcagcaaca ggttggcatt ttcccaattc atgccgttaa gtttttcaac catttcgctt
      421 gccgaaatgc acacatctgg tataggctgt ccaaaagcat cccgcacctc gttaatgttt
      481 aatggaggta aatttttgtg ttccactgta accgggtcaa agtaaactat cggcaccgca
      541 acacctgaga aaacattacg gtactggctc ataaaattta tgttcaagct gctaaaggtg
      601 tgcagctcca ggcaggcaac cagcttacga ttggggtatt cctccagaac agcgccaata
      661 gtggcggcaa ccttcgatgg cgagtgggca aaatcgagaa aaactgtgga gtttgctcct
      721 tcggctagtc gttgcagtct ttttgcagct cccttgaagg tggagattgc ccggtaaaaa
      781 ttcgattcgc taactccaag ttttaagcat acagccttag cagcgctaat gttctgcatg
      841 ttatggcgac caaaaatttt taaggggtag catgcctgct cggtaaccag ctggcagcca
      901 acgttgggct cgattcggta aggatgtgtt gtgtacggaa tcaactccag gtctgaacgt
      961 tttgtggagc ttactacccc tgctacctca tcatcctcgc tacagtaaac tagcgttccg
     1021 tttggctcaa tacagctgat gaaacccaca aattgatcac ggtaattttc aatggtaggg
     1081 aagacgttaa aatggtccca tgcaataccc gaaataactg caatggttgg cttgtaaaga
     1141 tggaatttcg ggcgtgggtc cacaggtgag gtcaaatatt catcgccctc aaatactgcc
     1201 aggttggttt ggttgttaag tccaaccata ttatcaaatc cctccagctg ggctcccacc
     1261 atataatcga aatca
//
LOCUS       DAOOQX010000655         1272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_146759_length_1272_cov_2.703369, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000655 DAOOQX010000000
VERSION     DAOOQX010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146759_length_1272_cov_2.703369"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLX53_09230"
     CDS             <1..796
                     /locus_tag="PLX53_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73305.1"
                     /translation="IPLSLNVNQLVASNDQTGNVPVVNEGVPAEGSLLIGQPNPVAYL
                     ENPEDDFALDSLILNYQNSNIEDAFDIAPNSFTLKSGITLGDASTSHDYFVRKNSNLT
                     LFSNVEVPLVGWVETNGIRDTVEIDLPDIENDLNMEDDDELSLTLKFRFNNGIPLNVY
                     FQAYFIDDYDHELTRLYTNVAEEQLIESAAVNPSTGKVTTPNVKISEISIDRNRYKQM
                     QGATKLVLQVRFKTGGSTHQNIVVESTNSIDIQMSIKAKGTVRFDS"
     gene            987..>1272
                     /locus_tag="PLX53_09235"
     CDS             987..>1272
                     /locus_tag="PLX53_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOY73306.1"
                     /translation="MKRLSLLALILTVGIYAKAQSELTLPFMPGVFQSSFVNPTVIPE
                     HTFSMGFSIYTSPEIRLTLKASGRKQMILCGFESHICVYQTALDLLRHGFG"
BASE COUNT          377 a          292 c          265 g          338 t
ORIGIN      
        1 gattcccctg tcgcttaacg ttaaccagct ggtagccagc aacgaccaaa ctggaaatgt
       61 gccagttgtt aatgaggggg ttcccgctga gggttcgttg ctcattggac agcccaatcc
      121 ggtggcctac cttgaaaatc ctgaagacga ttttgcgctg gactcactga ttctgaacta
      181 ccaaaactcc aacatcgaag acgcctttga tattgctccc aatagtttta ctctgaaatc
      241 gggaattacc cttggcgatg cctcaaccag ccacgactac tttgttagaa aaaactccaa
      301 ccttaccctt ttcagtaatg tagaagttcc gctggttggc tgggttgaga ctaacggcat
      361 tcgcgatacc gtagagatag accttccgga tattgagaac gatttgaaca tggaagatga
      421 cgacgagttg agcctaactc tgaagttcag gtttaacaac ggtattcccc tgaatgttta
      481 cttccaggct tactttattg acgattacga ccatgaactt accaggctat acacaaatgt
      541 ggcagaagag cagcttattg aaagcgctgc agtaaaccca tcaaccggaa aagtcacaac
      601 tccaaacgta aaaatttctg aaatttcgat tgacagaaat aggtataaac aaatgcaggg
      661 tgccaccaag ctcgttcttc aggtacggtt taaaacgggg gggagcactc atcaaaacat
      721 agttgttgaa agcaccaact ccatagatat tcagatgagc atcaaggcaa aaggaacggt
      781 tagattcgat tcctagtgtt aggagtatgc cgataaatag ctatacagct acaaatgaag
      841 cgcccacgat ttgatttaac gcaaccgtga ataagttaaa cttgaatatt tgcctttggc
      901 aagccttatg aactccaaat gctggtgaaa actaaaaagt gaaagcacaa cttattgtca
      961 actaataaca ttaacatact tttcggatga aacgtctgtc tttactagca ctaatactca
     1021 ccgtcggcat ttatgccaag gcccaatcgg agctaacgct accattcatg cccggtgttt
     1081 ttcagtcatc gttcgtaaat cctaccgtta tccctgaaca tacattcagc atgggcttta
     1141 gtatttatac cagtcctgaa attcgactta cgcttaaagc ttccggacgc aaacagatga
     1201 ttttatgtgg ctttgaaagc cacatctgtg tttatcaaac tgcgctggat ctgctccggc
     1261 acggcttcgg tg
//
LOCUS       DAOOQX010000656         1268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_147559_length_1268_cov_2.309151, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000656 DAOOQX010000000
VERSION     DAOOQX010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147559_length_1268_cov_2.309151"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PLX53_09240"
     CDS             complement(<1..415)
                     /locus_tag="PLX53_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73307.1"
                     /translation="MVKVKYHFNPHTLKFDAVSLPFYKRLAKILIHLGFYLLVFVVLG
                     YGFSMVFDTPIAQGLKRTNSEYSLKYEMALRQMQEFNDILTEIEQKDNNIYRAIFECD
                     SIPFSIRRGGYGGSERYSELVNNSNSHLLVKTFKIL"
     gene            566..>1268
                     /locus_tag="PLX53_09245"
     CDS             566..>1268
                     /locus_tag="PLX53_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOY73308.1"
                     /translation="MTSAEIRRAFLNFFEGKSHTIVASAPMVVKNDPTLMFTNAGMNQ
                     FKDIFLGNTPAKFKRVANSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     NEAIDWAWEFLVDVLKIDSTRLYATIFEGSPDEGVEKDYEALNCWAKHLPSNRIIEGN
                     KKDNFWEMGETGPCGPCTEIHIDLRGEDERKANPGEKMVNTGHPLVIEIWNLVFIQFN
                     RKANGQLEPLPAKHVD"
BASE COUNT          401 a          268 c          261 g          338 t
ORIGIN      
        1 caagaatttt aaaagtcttc accagcaaat gcgagttact gttgtttacc agctcggaat
       61 acctttccga acccccgtaa ccacccctcc taatggagaa cggaatggag tcgcattcaa
      121 aaattgcccg gtagatgttg ttatccttct gttcaatttc agtaagtatg tcattgaact
      181 cctgcatctg ccgcaaggcc atttcatact ttagcgagta ctcagaattg gtgcgcttca
      241 aaccctgcgc tattggagta tcaaacacca tggaaaaccc ataccccaaa acaacaaaaa
      301 ctaaaaggta aaatcccaaa tgaattagta tctttgccaa ccgcttataa aaaggcaggg
      361 aaacagcatc aaatttaagg gtatgagggt tgaaatgata ttttactttt accatccttt
      421 tttaactttt gtaactatgt ttgcctcctt tttgataaga tgcaacaaat ctatggtaaa
      481 aaatgaattt ttcaatgcca aaaataattt tttttttgaa aaatgattaa ttaataaatg
      541 ttaacctttt caaagcaagc attttatgac ttctgcagaa attcgtcggg catttttaaa
      601 cttttttgaa ggcaaatcgc acaccattgt tgcctctgca ccaatggtgg tgaaaaacga
      661 tccaaccctt atgttcacca acgccggaat gaaccagttt aaggatattt ttctgggcaa
      721 tactccggca aaattcaaaa gagtggccaa ctcgcagaaa tgccttaggg tttcaggaaa
      781 acataacgac ttggaagagg tcggtcatga tacataccac cacaccatgt tcgaaatgtt
      841 gggaaactgg tcgtttggcg actacttcaa gaacgaagcc attgactggg catgggaatt
      901 tctggtagat gtacttaaaa ttgacagcac caggttgtat gctacaattt tcgaaggaag
      961 ccccgatgag ggtgttgaaa aggactatga ggcgttaaac tgctgggcaa aacacctacc
     1021 ctctaatcga attattgagg gcaacaagaa ggataacttc tgggaaatgg gcgaaaccgg
     1081 accctgtggt ccctgcaccg aaatccatat agacctgcgt ggtgaagatg agcggaaagc
     1141 taaccccggg gaaaagatgg tgaataccgg ccacccgctg gtaattgaga tatggaacct
     1201 ggtttttata cagtttaacc gcaaggctaa tggccagctt gaacccctac ccgcaaagca
     1261 cgtagata
//
LOCUS       DAOOQX010000657         1267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_147704_length_1267_cov_3.050330, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000657 DAOOQX010000000
VERSION     DAOOQX010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147704_length_1267_cov_3.050330"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLX53_09250"
     CDS             <1..487
                     /locus_tag="PLX53_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOY73309.1"
                     /translation="DHASFYSKNIPVFFFTTGIHQDYHTPFDDVDKLNLTGMAQVTDY
                     AYRLIYKLSTMDSSLTFSKSGSRMVPTNHGNKVKVKLGIMPDVSGSTGNGLKVIAVSD
                     DKPAMQAGIQSGDIITSLGGVPVTNIYDYTEQLGKLEPGITVEVKVLRNNQELTFTLK
                     L"
     gene            513..>1267
                     /locus_tag="PLX53_09255"
     CDS             513..>1267
                     /locus_tag="PLX53_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73310.1"
                     /translation="MKKALLWIAAIIVTILAAIYQRDTGPTNPKRITYTIEGTTYKAK
                     LPRSGNSTSGCKVNLSLPLTSTATLYFRRYSTQDSFSPIPFIPEDEKTQSAYLPQMPP
                     AAKLEYYIVVNEGNNPMEIMRNSPVIIRFKGDIPAWALIPHILAMFLAMLYSSLAGLA
                     AAFKMESFRKFAIITLVLLLVGGFVLGPIIQFYAFGQAWTGFPLGFDLTDNKTLIALL
                     IWLAAVIANRKHSRPKIVIAAAFVTLVVFSIPH"
BASE COUNT          344 a          353 c          271 g          299 t
ORIGIN      
        1 cgaccacgct tcgttttact ccaaaaacat tccggttttc tttttcacca caggcattca
       61 ccaggactac cacacgcctt tcgacgatgt tgacaagctg aaccttacag ggatggcgca
      121 ggtaaccgat tacgcctacc ggctaatcta taagctatcc accatggaca gctcgctcac
      181 cttcagcaag tcgggtagcc ggatggtacc cacaaaccac ggcaacaagg ttaaggtaaa
      241 actgggtatt atgcccgatg tgagcggttc cacaggaaat gggcttaagg ttatagccgt
      301 ttccgatgat aaaccggcaa tgcaggctgg cattcaaagc ggtgatatca tcacctcttt
      361 gggcggagtt cctgttacta acatctacga ctacaccgaa cagctgggca agctggaacc
      421 cggcatcacg gtagaggtaa aagttcttag aaataaccag gagctgacgt tcacgcttaa
      481 actttagaaa ctacaaacta aaacataccg aaatgaagaa agcccttctc tggatagcag
      541 ccatcattgt aaccatcctg gcagctattt accaaaggga caccggcccc accaacccca
      601 aaagaattac ctacaccatc gagggaacca cctacaaggc aaaacttccg cgcagtggca
      661 acagcacatc cggctgcaag gttaacctaa gcctgccgct tacctcaacc gcaaccctat
      721 acttcaggcg ttactccaca caggatagct tctcgcccat ccctttcatc cctgaagatg
      781 aaaagacaca aagcgcatac ctgccccaaa tgccgcccgc tgccaaactt gaatactata
      841 ttgtggtaaa cgaaggtaac aaccccatgg aaattatgag gaatagcccg gtgattataa
      901 ggttcaaggg tgatatacct gcatgggcgc ttatccctca tatcctggcc atgttcctgg
      961 ctatgctcta ctccagcctg gcagggttgg cagctgcctt caaaatggaa tccttcagga
     1021 aattcgccat tattaccctg gtgcttttgc tggttggagg ctttgttctg ggaccaatta
     1081 tccagttcta cgcctttgga caggcatgga cgggctttcc ccttggattt gacctgaccg
     1141 ataacaaaac tctcatcgcc ctgctaattt ggcttgctgc ggttatagcc aaccgcaagc
     1201 actcccgacc caagatagta attgctgcag catttgttac gctagtggtt ttctcaattc
     1261 ctcatag
//
LOCUS       DAOOQX010000658         1267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_147741_length_1267_cov_2.638614, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000658 DAOOQX010000000
VERSION     DAOOQX010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147741_length_1267_cov_2.638614"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLX53_09260"
     CDS             <1..196
                     /locus_tag="PLX53_09260"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOY73311.1"
                     /translation="VSIVAALAINLLFMLYAKHIARFLNRFNLMNPLVRIFGLIVATI
                     GVQMTLNGIWGFVVSVAGHV"
     gene            268..1089
                     /locus_tag="PLX53_09265"
     CDS             268..1089
                     /locus_tag="PLX53_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOY73312.1"
                     /translation="MEQKNFIDIDQVIASKSPTLQKSLPRFIVNYLKRIVHQDEINEI
                     LAKHGHKQGAEFVEAVLSTMGVNYSVEGLENLQDNQRYLFASNHPLGGLDGMVLIHLL
                     AKRFDEVKFPVNDLLMHIKPLSSVFIPVNKLGTQSASNAIQLEEAYSSSAQILYFPAG
                     LCSRKQKGRIEDLEWKKNFIVKAIKHERDVVPVFFDGRNSTFFYNLARLRAKLRIKTN
                     IEMLYLSDEMFRQKGKSITVKIGKPIPFSTFDNTRNAHEWAQWVKHVVYQMKPAS"
BASE COUNT          381 a          292 c          270 g          324 t
ORIGIN      
        1 tgtttccatt gttgctgccc ttgccattaa cctcctcttc atgctctacg ccaagcatat
       61 agcaagattc ttaaaccgtt tcaaccttat gaatccactg gttagaattt ttggacttat
      121 agtggccact attggggttc aaatgacgct aaatggaatt tgggggtttg tggtttcagt
      181 tgctggacac gtgtaacttc gagctaataa tcaccacaaa aaatcctcat acattgctat
      241 atttgcagaa cataagaaac attatcggtg gaacaaaaaa attttataga cattgatcag
      301 gttattgcct caaaaagccc tacgcttcaa aaaagcctcc cccgtttcat tgtcaactac
      361 ctaaagcgta tagttcatca ggacgaaata aatgagattc tggccaagca tgggcataag
      421 cagggagctg agtttgtgga ggcagttctc agcacaatgg gggtaaacta ctcggttgag
      481 gggttggaaa acctgcagga taaccaacgc tacctttttg catcgaacca cccgctcggt
      541 gggctcgatg gcatggtgct tattcacctg ctggcaaaaa ggtttgacga ggtgaagttt
      601 cccgtaaacg acctgctcat gcacattaaa cccctctcca gcgtattcat accagttaat
      661 aagcttggaa cgcagagcgc ttcaaacgcc attcagcttg aggaggctta cagctcatcg
      721 gcacaaattc tatactttcc tgccggcctt tgctcacgca agcaaaaggg tagaattgag
      781 gacctggaat ggaaaaaaaa ttttattgtt aaagccatta aacatgagcg ggacgtggtg
      841 ccggtatttt ttgacgggcg taactccacc tttttttaca acctagccag gttacgggct
      901 aagctgagga ttaaaaccaa catcgagatg ctctaccttt ccgatgaaat gttcaggcaa
      961 aaaggaaaga gcattacggt taaaataggt aagcctatcc cattcagcac ctttgacaac
     1021 accagaaatg cccatgaatg ggctcaatgg gttaagcatg tggtttacca gatgaagccc
     1081 gcttcataaa ctgtcaaatt gatgcagtaa aaaccaaccc agtaaactcc aattctagaa
     1141 gatactagcc atgcaggaaa taatcgaacc ggttgaccgg aacctactaa tggcagaact
     1201 cacccccgag aagttcgtta ggaacaccaa caatgcggaa aatgagctct acctctttac
     1261 cgcccat
//
LOCUS       DAOOQX010000659         1266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_147910_length_1266_cov_3.232865, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000659 DAOOQX010000000
VERSION     DAOOQX010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147910_length_1266_cov_3.232865"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLX53_09270"
     CDS             <1..358
                     /locus_tag="PLX53_09270"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOY73313.1"
                     /translation="RKNMAILMLGFAIGIISGVFGAGGGGMILLILVFVLNFPIRVAI
                     GTSSLLMAITSFSGALGYFIHGNIRLVPGLVMGSGAALSGVVSARFANRVNEDILSIA
                     VGVVFFMLGTLMILIA"
     gene            591..>1266
                     /locus_tag="PLX53_09275"
     CDS             591..>1266
                     /locus_tag="PLX53_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOY73314.1"
                     /translation="MFFWVLPGVAVAQARPTSLQKPNGTAIIITGAAAKIAQEAALLQ
                     NLYEKGMLKNVVFISGASSGALNAVMLNAILEGKLTWKDYKSILGQLSNEKVFKPDGN
                     RLPVNTTPLRNTISSIVCDRLGYRILSDLPIPTSISVVNFKIAPFSDRTSRLCNLKIN
                     PESDPSLNLVDVLMASTSIPVIFPPSPIKNVRTIPNVPYYDGGIAADHVPYQALVEYE
                     KFRGVPV"
BASE COUNT          325 a          265 c          303 g          373 t
ORIGIN      
        1 gcgtaagaat atggcaattc ttatgctggg ttttgccata ggcattattt ctggagtttt
       61 cggtgccggg ggaggaggaa tgattctttt gattttagtt tttgtgctta actttcccat
      121 aagggtagct ataggaacat cgtcgcttct tatggctata acttcgttct cgggagcact
      181 tggttacttt attcatggca atataaggct tgttcctgga cttgtgatgg ggtcgggagc
      241 tgccttgagc ggcgtagtca gcgccaggtt tgccaatagg gttaacgagg atattctttc
      301 cattgctgtt ggggtggtat tttttatgct ggggacttta atgatactga tagcatgatt
      361 tgactctttg ctgtggcagc agccgttgtg gggggatgct ttaacattgg actaagccta
      421 ttttgaagta aaatgacggc aagtatcctt aagccttaag ccagacaacc aaagctatat
      481 agcagcgttt ggacatgagc taatttcata cgaagattat gacgaattta tggctgtgct
      541 ggttaaaacc cgctttcttt tcagtaaaaa ggtattgcat tgtagcactg atgttttttt
      601 gggtattgcc aggtgtagca gttgcccaag ccaggccaac cagccttcaa aagcccaacg
      661 gcactgcaat tataataaca ggagctgctg caaaaatagc ccaggaggca gcccttcttc
      721 agaatctcta cgaaaaggga atgcttaaaa acgtggtatt tatttctggc gccagctccg
      781 gggcgcttaa tgctgttatg ctgaacgcaa ttcttgaggg taaattaacg tggaaggatt
      841 acaagagcat tctgggtcag ctatcgaacg agaaggtttt taaaccggat gggaacaggt
      901 tacccgtaaa tacaaccccg ctaagaaata ctattagcag cattgtgtgc gataggttag
      961 gctaccgaat tctttccgat ttgccgattc ccacttccat ttcggtggtt aacttcaaaa
     1021 tagccccttt cagcgataga acatcccggc tttgcaacct aaaaattaac cctgaaagcg
     1081 atccttcgct caacttagtt gatgttctga tggcatcaac ctcaatcccg gtaatttttc
     1141 caccgtcccc cattaaaaat gtaagaacca tacctaatgt tccctactac gacggcggaa
     1201 ttgccgccga ccatgtgcca taccaagcac tagttgagta tgagaaattt cggggtgtgc
     1261 ccgtcg
//
LOCUS       DAOOQX010000660         1265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_148174_length_1265_cov_2.436364, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000660 DAOOQX010000000
VERSION     DAOOQX010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148174_length_1265_cov_2.436364"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1265)
                     /locus_tag="PLX53_09280"
     CDS             complement(<1..>1265)
                     /locus_tag="PLX53_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOY73315.1"
                     /translation="ITQPDGTKISCFVSGDEFHNWLHDAAGYTIMRNPANGFYVYAVE
                     KNGKLTSSDYVVGKVNPRNSKDLKPYVNISRNEYLAKRHRKQTDNHHVNLDITKGTSG
                     IAPLRAVKGVTMNNIVVFIRFKDDVVADENISYYENVYNGSSASVANYYSTVSYGKFS
                     VSSTFYPQLNGTLVTWYVDENNRNYYQPYDATTNPNGYNPDASNYNDPTSSTYREHTL
                     LKKAVEAVGASIPTGINLDINGDNYIDVISFILAGSNDSWNDLLWPHQWSLWSQSAFI
                     NGKQVGDYTLQLQYFGGSQIDLGTLCHEMFHAVGAPDLYHYDDDLNLSPVGYWDIMEG
                     TLDYPQNMGAHMKYLYGGWIDQIPEITTSGTYTLSPLATSPSGNSYKIPSPNSYSEYF
                     VVEYRKKESYDSDLPGNGLLVYRINSKLE"
BASE COUNT          299 a          276 c          333 g          357 t
ORIGIN      
        1 ttcaagcttc gagtttattc ggtacaccag aagtccgttg cccggtaaat cactgtcgta
       61 ggattccttt ttcctgtact ccactacaaa gtactcactg tagctgttgg gcgaggggat
      121 tttgtagctg ttaccggatg gagatgtggc caaaggtgaa agcgtgtagg tgccggaagt
      181 ggtaatttca ggtatttggt caatccaacc cccgtaaagg tacttcatgt gggcacccat
      241 gttctggggg tagtccaggg ttccttccat gatatcccaa tagccgacag gactaaggtt
      301 tagatcatcg tcgtagtggt ataggtcggg tgcgccaact gcatggaaca tttcgtggca
      361 gagggtaccc aggtcaattt gtgaacctcc gaagtactgc agctgaaggg tgtaatcgcc
      421 aacctgttta ccgttaatga atgcactctg cgaccagagg ctccactggt ggggccacag
      481 caggtcgttc cacgagtcat tgctaccggc taaaatgaac gaaattacgt caatgtagtt
      541 gtcgccattt atatcaaggt taattccggt tggaatgctt gccccaacag cctccaccgc
      601 ttttttcagc agggtgtgct cccggtaggt tgaactcgta ggatcgttat agttcgaagc
      661 gtcggggttg tagccatttg ggtttgtggt ggcatcgtag ggttggtagt agttccggtt
      721 gttctcgtca acataccagg ttacaagggt gccgttgagc tgggggtaaa aggtggagct
      781 aacactgaac ttaccgtacg aaacagtaga gtagtagttg gctaccgatg ctgacgaacc
      841 gttgtaaaca ttttcgtagt aggaaatatt ctcatcggct acaacatcat ctttaaaacg
      901 gataaaaacc acaatgttgt tcatggtaac acccttaacc gctctaagtg gggcaatacc
      961 gctagtccct tttgtaatat caaggtttac atgatggttg tcggtttgct tgcggtggcg
     1021 tttagccagg tactcattac gcgaaatatt cacgtagggc tttaaatcct ttgagtttcg
     1081 aggatttact tttcctacaa catagtctga tgacgtcagc ttcccattct tttcaacagc
     1141 ataaacgtag aacccgtttg caggattacg catgatggtg tagcctgcag cgtcgtgcag
     1201 ccagttatga aactcatcgc cgctgacaaa gcagctgatt tttgtgccgt cgggctgggt
     1261 gatgg
//
LOCUS       DAOOQX010000661         1264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_148314_length_1264_cov_2.893300, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000661 DAOOQX010000000
VERSION     DAOOQX010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148314_length_1264_cov_2.893300"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLX53_09285"
     CDS             <1..184
                     /locus_tag="PLX53_09285"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HOY73316.1"
                     /translation="NTASSKMILLLLSKLKDLSLLGKNVRVTWCFPSHDSEMEEAGEE
                     LAEILNIPFDFIPKNS"
     gene            202..609
                     /locus_tag="PLX53_09290"
     CDS             202..609
                     /locus_tag="PLX53_09290"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOY73317.1"
                     /translation="MEKLYIEPKKDSPEVIFDPKNGNFSITGISHPENVATFFEPIFS
                     WLEAYFVELGRTDSIRMKPINVRLFFKYINSATFKQLVTLMQKFKQFVETGYKVNITW
                     QFEPDDEDMKEAGYELIEISGINTTFSCEVCNN"
     gene            complement(693..>1264)
                     /locus_tag="PLX53_09295"
     CDS             complement(693..>1264)
                     /locus_tag="PLX53_09295"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOY73318.1"
                     /translation="NRVLILEVMGRFAGWIALHAAIAGGAEVCLIPEIPYDINKVAEK
                     LNSRYNKGKGHAIVVIAEGAKPIGGTLTSEQSDEVGYNNLRLGGVAHSLTNDLKRIGF
                     EADIRETVLGHLQRGGIPVAYDRVLATQFGVKAFEMVLQGKFGEMVAYRHPDIISVPL
                     IDAINRPNYVDPNCDLVRTARGVGISFGD"
BASE COUNT          363 a          291 c          250 g          360 t
ORIGIN      
        1 caacacggcc tcatcaaaga tgatactcct cctgctcagc aagcttaaag atttatccct
       61 acttggcaaa aacgttcggg ttacctggtg cttcccttct catgattctg aaatggagga
      121 agcaggtgag gagctggccg aaattcttaa cattcctttt gattttattc caaaaaacag
      181 ctagcctgga actaatttaa aatggaaaag ttatatatag aacctaaaaa ggattcaccg
      241 gaggttatct ttgaccccaa aaatggaaat ttctccatta ccggtatttc ccaccccgag
      301 aatgtggcta ctttttttga gccaatattt tcatggctcg aagcttattt tgtagaattg
      361 ggaaggaccg attccatccg gatgaaaccc ataaacgtta ggttattttt caagtacatc
      421 aactccgcta ccttcaagca gctggttacg cttatgcaga aatttaaaca gtttgttgaa
      481 acgggataca aggtaaacat tacctggcag tttgagcctg acgatgagga tatgaaggag
      541 gctggttacg agttgatcga aatatcaggc attaatacta ccttttcctg cgaggtttgc
      601 aacaactaga taatttttat tcttcatgta gaacataaag aaaaaaagga acttagccaa
      661 agttcctttt actgttgctg ttataattca aattaatctc caaaactaat accaacaccc
      721 ctggcagtgc gaaccaaatc gcagttggga tcaacgtagt ttggacggtt aatagcgtca
      781 ataaggggta ccgaaataat gtcggggtga cggtaggcta ccatttcacc aaattttccc
      841 tgaagcacca tttcaaaagc tttaacccca aactgggttg ccagtacacg atcgtatgct
      901 actgggattc ctcctctttg aagatgtcca agcactgttt ccctaatatc tgcctcaaag
      961 ccaatccttt tcaaatcgtt agttagcgag tgggctactc caccaagacg taggttgttg
     1021 tatcccacct catcgctctg ctccgaagtt agagtgccgc ctattggttt tgccccttcg
     1081 gcaataacca caatggcgtg acctttcccc ttattatagc ggctgttaag cttctctgca
     1141 accttattga tgtcataggg aatctctgga ataaggcaaa cctctgctcc tcctgctatg
     1201 gctgcatgaa gggctatcca tccggcaaag cgacccataa cctcaagaat aagcaccctg
     1261 ttat
//
LOCUS       DAOOQX010000662         1262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_148653_length_1262_cov_3.720795, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000662 DAOOQX010000000
VERSION     DAOOQX010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148653_length_1262_cov_3.720795"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1262)
                     /locus_tag="PLX53_09300"
                     /pseudo
     CDS             complement(49..1262)
                     /locus_tag="PLX53_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647187.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
BASE COUNT          317 a          326 c          272 g          347 t
ORIGIN      
        1 gctggctaaa aacattgttg attacaggaa gcagaatggc ccattcttca gctccgttcg
       61 gcttctgaat gggccattct gcttcctgta atcaacaatg tttttggcca gctgtggtcc
      121 taagccagag acataggtta gcaaatgcat gctggcagta ttcaggttaa cccctacatg
      181 gttaacacaa ctctcaacaa ccctgtcaag cccctcttta agctttgact ggtcaacatc
      241 gtgctggtac tgccctaccc caattgattt tggatcgatt ttaaccagct cggcaagcgg
      301 gtccatcagc ctacgaccaa tggaaactgc accccgaaca gttacatcat agtcgggaaa
      361 ttcctcacgg gcaactggag aagcggagta aacagatgcg ccatcctcgc tgaccacaaa
      421 gaccttaaca tctcggttga aatgaatacg ctttacaagc gactctgttt cgcgaccggc
      481 ggttccgtta ccaatggcta ttgcatcaat tttgaatgtt tcaacaaggc ttgaaatttt
      541 tgacatagcc tttccctgct cgttgcgggg ttgatgtgga taaatggttt cgttatggat
      601 aaggtttccc tgagcatcaa ggcaaaccat cttgcagccc gtacggtagc ccggatcaat
      661 agccagcaca tttttctgtc ccaacggagg ggaaagcagt agctggcgaa ggttttcggc
      721 aaatactcgt attgcctcag catccgcctt ttcctttagc tcggcagtaa actcattttc
      781 aattgatggt tctatcaacc ttttatagga atcggctagt gcaagttcga gctgcttggc
      841 tgtttcaccg gttgacttca gcagcgattt ccgtagctca tcaagaacgc catcggcagg
      901 aggggttatg gaaacccgga gaaaaccctc atcttcgcct cgtttaatag ccaaaaacct
      961 gtgagaagcg cagcgtcgga caggctcttc aaactcaaag taatcggaat attttatgcc
     1021 ttcggcttcc ttccccttga caactcttga acgaataatg gctttctgct taaaattgcg
     1081 gcgtacagta tttctaactc tttcatcctc gcttagcatt tcggcagcta tatcgcgcgc
     1141 accctgtaac gcctcctcaa cattggcaac cttatcggat ataaaggttt gggcttttgt
     1201 taaaatatcc ttttcaacct gattaaacac cagcaaggca agcggttcca gacccttctc
     1261 cc
//
LOCUS       DAOOQX010000663         1262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_148675_length_1262_cov_3.096935, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000663 DAOOQX010000000
VERSION     DAOOQX010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148675_length_1262_cov_3.096935"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLX53_09305"
     CDS             <1..423
                     /locus_tag="PLX53_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOY73319.1"
                     /translation="GFVPSPFKLKDVNGHEVTLDQLEGKYVYLNFCTTTSYTCLQDFS
                     LLEKIYRKYRKKLEIVTISADMEKKDLESFLKTTGYSWTFLHFGEKPDVIKDFDVRAY
                     PTYFLIGPDRKIILSPAPSPREGFEQRFFELLRSRGEI"
     gene            complement(420..1043)
                     /locus_tag="PLX53_09310"
     CDS             complement(420..1043)
                     /locus_tag="PLX53_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOY73320.1"
                     /translation="MASEYVIFFNDRRFTITSDLAGYFVGNEKGYFLGHASSYDIPKL
                     VKIFQSHPAICNVFVGNGSVDKLFSWFTSQFKFLAAAGGLVRNNAGQYLMIYRRGKWD
                     LPKGKVEPSETPVEAGLREVEEETGISGLTVVRPLTCTYHTYEEKGNTMLKQTHWFLM
                     QVRGTLSLKPQVEEDIEIAEWVDADGVENRLKDSYPSIRMVFASLRS"
     gene            1024..>1262
                     /locus_tag="PLX53_09315"
     CDS             1024..>1262
                     /locus_tag="PLX53_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOY73321.1"
                     /translation="MTYSEAINAESIAANLLKIKAIRLSPTAPFTWASGLKSPIYCDN
                     RITLSYPDVRNRICNAFVELVRRLYPDVELVAGVAT"
BASE COUNT          338 a          307 c          278 g          339 t
ORIGIN      
        1 ggatttgtgc caagtccttt caagctgaag gatgtaaacg gccatgaagt tacgctcgac
       61 cagcttgagg gaaaatacgt ttatctgaac ttctgcacca ctaccagcta cacatgcctt
      121 caggattttt ccttgctgga aaaaatctac cggaagtata gaaaaaaatt agaaattgtt
      181 accatttcgg cggatatgga gaaaaaggat ttggaaagtt ttcttaagac taccgggtac
      241 agctggacat tcctgcactt tggagaaaaa cccgacgtga ttaaggattt tgatgtgagg
      301 gcttacccca cctacttcct gattggcccc gatagaaaaa ttatactttc tcccgctcct
      361 tcgccaaggg aagggttcga gcagcgattt tttgagctgc tccggagcag gggcgaaatc
      421 tagcttcgta aactcgcaaa taccatccgg atagatgggt aggagtcttt caaacggttt
      481 tcaaccccat ctgcatcaac ccattcagct atttcaatat cttcctcaac ctgaggcttc
      541 aggctgagag tgcccctaac ctgcatcaaa aaccagtggg tttgcttgag catagtgttt
      601 cccttctcct cgtaggtgtg gtaggtgcat gtaagcgggc ggacaaccgt tagacctgaa
      661 attcctgttt cctcctccac ttcgcgcaag cctgcctcaa caggggtttc tgaaggttcg
      721 accttcccct ttggtagatc ccattttccg cgtcggtata tcatcagata ctgtccagcg
      781 ttattccgca ccaaccctcc ggcagctgca aggaacttaa actgagaggt aaaccaggag
      841 aagagcttat ccacgctgcc gttaccaaca aaaacattac aaattgccgg gtgactttga
      901 aaaattttga ctaactttgg aatgtcatag ctggaggcat gtcccaaaaa ataccctttt
      961 tcgttgccaa cgaagtaacc agccaaatcc gatgtgatgg taaacctcct atcgttgaaa
     1021 aatatgacgt actctgaagc cataaatgct gaatcaatcg ctgcaaattt actaaaaatt
     1081 aaggcaattc gtttaagtcc gacagctcct tttacctggg cttcgggtct taaatcgcca
     1141 atatactgcg ataaccgaat aaccctttcc tatccggatg ttcgaaaccg gatatgcaac
     1201 gcctttgtgg agcttgttcg tcggttgtat cccgacgtcg agctggttgc gggagtggct
     1261 ac
//
LOCUS       DAOOQX010000664         1257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_149747_length_1257_cov_2.927621, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000664 DAOOQX010000000
VERSION     DAOOQX010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149747_length_1257_cov_2.927621"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PLX53_09320"
     CDS             <1..818
                     /locus_tag="PLX53_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOY73322.1"
                     /translation="TGFYRETIKQRRDYVSIAEAVTEIYKSPYKFTLESDRIKVIKGR
                     KGDNVKKADTLIVKMQGGPNVSLYLDIVRYPELILGLEEMKHYQYELTDMVSIDNRSN
                     YVISFKPLAKLEYPLYYGKIYVDVESLAITMAEFSMDISDVEKATQAFVRKKPAGLIF
                     EPVSTSYLVTYKKLGDRYYLNYMRSEIKFQADWKKKIFKTSYTLMSEMAITERDTVNA
                     AKIAAKESFKPYAILTDKVNEYFDEDYWGSYNTIEPDESIQSAIAKYNRRFKR"
     gene            905..>1257
                     /locus_tag="PLX53_09325"
     CDS             905..>1257
                     /locus_tag="PLX53_09325"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HOY73323.1"
                     /translation="MSLNGLNRQSRIKDDSIGDFEVLFKTYYVPLCRFAFSIVRDSDQ
                     AEEIVQEFFYNYWKNRKNIGIKFSIRAYMYKAVKNSALRYLAHRKVESSYGVTLGSTS
                     GGFDNLDESSDELLK"
BASE COUNT          371 a          276 c          278 g          332 t
ORIGIN      
        1 tgaccggttt ctatcgcgaa accattaagc agcgtcgcga ctatgtgtcc attgccgaag
       61 ctgttaccga aatctacaaa tcaccctata agtttaccct ggaatcggat aggattaagg
      121 ttatcaaggg gcgtaaaggc gataacgtga aaaaggccga tacccttatc gtgaaaatgc
      181 agggtggccc caatgtttcc ctttatctgg atatagtcag gtaccccgag cttatactgg
      241 gattggagga aatgaagcac taccaatacg agcttaccga tatggtcagc atcgacaacc
      301 gttccaacta tgttatttca tttaagccac ttgctaagct cgaatacccg ctttactacg
      361 gtaagatata cgttgacgtg gagtcgctgg ccatcaccat ggccgagttt agcatggata
      421 tttcggatgt tgaaaaggct acccaggcat ttgtcaggaa aaaacctgcc gggctgatct
      481 tcgaacccgt cagcaccagc tacctggtta cctacaaaaa gttgggcgac aggtactacc
      541 taaactacat gcgcagcgaa attaaatttc aagccgactg gaaaaagaag atttttaaaa
      601 ccagctacac cctcatgtcc gaaatggcca ttaccgaacg tgacaccgtt aatgctgcca
      661 agattgctgc aaaggaatcg tttaaacctt atgccatttt aaccgacaag gtaaacgagt
      721 actttgacga ggactactgg ggctcatata acaccattga gcctgatgaa tccattcaat
      781 cggctattgc caagtacaac cgtaggttta aaaggtaacc ctatgggcat cttagcctac
      841 ctattgtttc tgcccgtagc caattatttt atacctttgg actaataccg tagcctgatt
      901 atagatgagc ctgaacgggt tgaaccggca aagcaggatt aaggatgata gcattggtga
      961 ttttgaggtt ctcttcaaaa cctactatgt cccgctttgc cggtttgcct tttcaatcgt
     1021 cagagattcc gatcaagccg aagaaattgt tcaggaattt ttttacaact actggaaaaa
     1081 ccgtaaaaat atcggcatca agttttcaat cagggcatat atgtacaaag ctgttaagaa
     1141 tagcgcattg cgatacctgg cacatagaaa ggtggagagc agctatggtg ttaccttagg
     1201 tagtacatct ggaggttttg ataatcttga tgagagctcc gacgagctgt taaaaat
//
LOCUS       DAOOQX010000665         1256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_149939_length_1256_cov_2.761865, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000665 DAOOQX010000000
VERSION     DAOOQX010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149939_length_1256_cov_2.761865"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLX53_09330"
     CDS             <1..324
                     /locus_tag="PLX53_09330"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73324.1"
                     /translation="KITGGKPKIIGSGGNINKLYRLAGKVEGRPLSFAELQKLFAQLK
                     SYTFEERISILGMNPDRADVIIPAARIYLKTMEWTGASKILVPTIGVSDGIVRMLYKN
                     YRSRS"
     gene            452..>1256
                     /locus_tag="PLX53_09335"
     CDS             452..>1256
                     /locus_tag="PLX53_09335"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOY73325.1"
                     /translation="MVEQRKRVAFYTLGCKLNFSETSTIARQLVEMGYERVSPDDKAD
                     VYVINTCSVTEHADKKCRQAIRKFASRSPGALIAVVGCYAQLKPDEVAAIEGVDLVVG
                     ASAKFQLPQLLATHVGKDGAKVYSCDIDRVADFSPAYSSGDRTRSFLKVQDGCDYHCS
                     YCTIPLARGKSRNMPVTNIVAQAQAIAAQNVKEIVLTGVNTGDFGRSTGESFIELLKE
                     LDAVEGIERYRISSIEPNLITDEIAHFVASSKKFLPHFHIPLQSGSNRIL"
BASE COUNT          338 a          295 c          313 g          310 t
ORIGIN      
        1 aaaattaccg gcggaaagcc taaaattata ggctcgggcg gaaacatcaa caagctctac
       61 cggctggctg gaaaggttga gggaaggccg ttgtcgtttg ccgagcttca gaagctattc
      121 gcacagttaa aatcctacac ctttgaagag cgtatcagca tcctgggaat gaaccccgat
      181 agggctgatg tgattattcc tgccgccagg atttacctga aaacgatgga gtggaccggg
      241 gccagtaaaa tactagttcc caccattggc gtttcagatg gtattgtgcg aatgctttac
      301 aagaattacc ggagtagaag ctagcgcagg ttttctactt ttctatccat acaaattgtg
      361 caagcggttt acttttaggg gcaatttgca cgctacgagc caatcatcaa tctcgccaat
      421 gattgctacc tttgcaaaaa ataactgaat aatggtggag cagcgcaaac gggttgcctt
      481 ttacacgcta ggatgtaagc ttaacttttc cgaaacgtca accattgcca ggcagcttgt
      541 cgaaatgggg tacgaaagag tttcgcccga cgataaggcc gatgtttacg tgattaatac
      601 ctgctcggtt accgagcatg ccgataagaa atgcaggcaa gccatcagga agtttgccag
      661 tcgttcgccc ggcgccctga tagccgtagt gggttgctac gcccagctta aacccgatga
      721 ggttgccgcc attgaagggg ttgacctggt agtaggcgct tcagccaaat tccagctgcc
      781 ccagctgcta gctacacatg taggtaagga tggagcaaaa gtttacagct gcgatattga
      841 cagggtagcc gatttttctc cagcttactc ctcgggcgat cgaacccgtt cgtttcttaa
      901 ggttcaggat ggatgcgact accattgcag ctactgcacc ataccgcttg ccagagggaa
      961 aagcaggaat atgcctgtta ccaacattgt ggcacaggct caggcaattg cagcacaaaa
     1021 cgttaaggaa atagttctta caggggttaa taccggcgat ttcggtaggt caaccggtga
     1081 aagtttcata gagctgctaa aagagctcga tgccgttgag ggtattgaac gctacaggat
     1141 ctcctccatc gagccaaacc ttataaccga tgaaatagcc cattttgtgg ccagctcaaa
     1201 aaagtttctg cctcatttcc atattcctct tcaatcgggt tcgaacagaa tactcg
//
LOCUS       DAOOQX010000666         1256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_150009_length_1256_cov_2.178185, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000666 DAOOQX010000000
VERSION     DAOOQX010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150009_length_1256_cov_2.178185"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLX53_09340"
     CDS             complement(<1..661)
                     /locus_tag="PLX53_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOY73326.1"
                     /translation="MKRIAILGGGESGAGAAVLAFKKGFDVFLSDSSTIKPRYREWLQ
                     RYSIPFEENGHTEALILNADEVIKSPGIPDKASIVARIREQGIPVISEIEFAGRYNTA
                     RTICITGSNGKTTTTSLIYHMMQKAGLNVGLAGNIGNSFAYQVAEDNFDYYVIELSSF
                     QLDGVYEFKPDIAILLNITPDHLDRYDYKMQNYVESKFRITRNLSEDDCFIFCSDDAV
                     TV"
     gene            complement(809..>1256)
                     /gene="mraY"
                     /locus_tag="PLX53_09345"
     CDS             complement(809..>1256)
                     /gene="mraY"
                     /locus_tag="PLX53_09345"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOY73327.1"
                     /translation="SGNVIYSEYLNIMYIPHISELVVFMSALIGALIGFLWYNSYPAQ
                     VFMGDTGSLTIGGIIAVYAIVVRKELLLPILCGIFLVEALSVLIQVSYFKVTRKIYGE
                     GRRVFLMSPLHHHYQKKGYAEPKIVTRFWIISMLLAVITVVTLKIR"
BASE COUNT          333 a          307 c          283 g          333 t
ORIGIN      
        1 caacggtaac ggcatcgtcg gaacaaaaaa tgaagcaatc atcctctgat aggtttcggg
       61 tgattctgaa cttcgattcc acatagttct gcatcttgta gtcgtatcgg tcgagatgat
      121 caggggtgat gttcagcaga atggcaatgt cgggtttgaa ctcgtaaacg ccatcgagct
      181 ggaagctgct gagctcaata acgtagtagt cgaaattgtc ttcggccacc tggtaggcaa
      241 agctgttgcc tatgtttcca gccaggccca catttagccc cgccttctgc atcatgtggt
      301 atatcaacga ggtagtggtg gttttgccat tgctgccggt tatgcagatg gttcgggctg
      361 tgttgtatcg tccggcaaac tcaatctccg aaataacagg gataccctgc tccctaattc
      421 ttgcaacaat tgaagcctta tcgggaatgc cgggactttt aatcacctcg tcggcgttaa
      481 gaatgagcgc ctctgtgtgg ccgttttcct caaaaggtat gctgtacctt tgcagccact
      541 cacggtagcg gggctttatg gttgatgaat ccgatagaaa aacatcaaag cccttcttga
      601 atgccagcac agccgctccg gcaccgctct ctccacctcc cagaatagct attcgcttca
      661 ttttaaagaa atatttgtat tcattcgttt ccggctagct gcagccatct gccacggtag
      721 ccaattatag ggtatcctcc ctacgttaac ctttttatac cgtatgatac catttagccc
      781 catatttgca ccagctaaac acatataact acctgatttt taaagttacc acggtgataa
      841 cagctagcaa catgcttata atccaaaacc tggtaacaat tttaggttcg gcataaccct
      901 ttttctggta gtggtggtgg agaggcgaca tgagaaatac ccttcgtccc tccccgtaaa
      961 ttttccgggt aaccttaaag tagctgacct ggatgagaac tgaaagcgct tcaaccagaa
     1021 agattccaca gagtatgggc agcaggagtt ccttacgaac aacaatggca taaacggcta
     1081 taatgccacc tatggtaagg ctcccggtat cgcccataaa cacctgggcg gggtagctgt
     1141 tgtaccagag aaatccaata agcgccccta tgagtgcgct catgaaaaca accaactcac
     1201 taatgtgcgg tatatacatg atgttgagat actcagagta aataacgtta cccgag
//
LOCUS       DAOOQX010000667         1254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_150296_length_1254_cov_3.766472, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000667 DAOOQX010000000
VERSION     DAOOQX010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150296_length_1254_cov_3.766472"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PLX53_09350"
     CDS             complement(<1..341)
                     /locus_tag="PLX53_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOY73328.1"
                     /translation="MTKKSLIFFLYFWVTLIPVKADEGMWLPLLIGKNTSNMQALGCK
                     LTSEEIYSVNHSSLKDAVVQFGGGCTAEIISPDGLIITNHHCGFSSIQSHSSVQNDFL
                     TNGFWAMSRSE"
     gene            complement(346..948)
                     /locus_tag="PLX53_09355"
     CDS             complement(346..948)
                     /locus_tag="PLX53_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOY73329.1"
                     /translation="MLLQELLTGYKLVLASGSPRRKDLLGGLNIPFEVWPTEHQDESF
                     PSGLDTEIIPQYLAKKKSLAFTGKLDNSTILITSDTIVACNGMVLGKPADMVEASNML
                     KLLSGNMHTVVTGVCISSSSRSATFQSSTNVYFRNLSAEEIDYYVQHYNPLDKAGAYG
                     VQEWIGFVGVERIEGSYFNVMGLPVQRLYIELVSFLQNNS"
     gene            complement(958..>1254)
                     /locus_tag="PLX53_09360"
     CDS             complement(958..>1254)
                     /locus_tag="PLX53_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73330.1"
                     /translation="PVRYGIKTAKVTAASMLGITMLALVYLFAAYMRQLSTGEFDFVT
                     FSYFFLFIVVPCFVLLVMILLAQEKKDYHRASGFCKLIMLFGLLYAFIFRWFIV"
BASE COUNT          390 a          268 c          274 g          322 t
ORIGIN      
        1 tcttcggagc gagacatggc ccaaaaacca tttgtaagaa aatcgttttg taccgatgag
       61 tgcgattgaa tggaagaaaa tccgcaatga tgattggtaa taattaatcc atcgggtgaa
      121 ataatttcgg cagtgcagcc accgccaaac tgcaccacgg catccttaag gctggagtgg
      181 ttaacgctgt agatttcttc ggaggttagc ttgcacccca gggcctgcat gttggaagta
      241 ttttttccaa tcagcagggg aagccacata ccctcatcgg ccttaaccgg aataagggtt
      301 acccaaaaat acaggaaaaa aattaatgat tttttagtca tagttttagc tattattttg
      361 taaaaaacta accagctcaa tatataggcg ctgtacgggc aatcccatta cgttaaagta
      421 ggagccttct attctttcga cacctacaaa cccaatccac tcctgtactc cataggctcc
      481 agccttatca agtgggttat agtgctgtac gtagtagtct atctcttcgg cagaaagatt
      541 tcgaaagtaa acattcgtgg aggattgaaa agtagccgac ctactactgg agctaatgca
      601 gactccagta acaacggtat gcatgttgcc cgaaaggagc ttcaacatgt tactagcctc
      661 caccatatca gcaggtttac ccagcaccat tccgttacat gctactattg tgtcggaggt
      721 tatcagtata gttgaattat cgagtttgcc agtaaaggct aacgattttt ttttagctag
      781 gtactggggt attatttcag tgtccaaacc tgaagggaaa gactcatcct gatgttcggt
      841 aggccatacc tcaaatggga tattcaatcc cccaagcaaa tcttttcgtc tgggagagcc
      901 cgaggcaagc actagcttgt atcctgtcaa aagttcttgt aaaagcatca ataaaagtta
      961 aacgataaac caacggaaaa taaatgcgta gagaaggcca aatagcatga taagcttgca
     1021 aaagccactt gccctgtggt agtccttctt ttcctgcgcc agaagtatca tgaccagcag
     1081 cacaaagcat ggaacaacta tgaataaaaa gaagtaggaa aaagtaacaa aatcgaattc
     1141 gccggtgcta agctgacgca tgtaagcggc aaaaaggtaa accagagcaa gcatggtgat
     1201 tccaagcatg gaagctgctg taaccttggc tgttttgata ccatatcgga cagg
//
LOCUS       DAOOQX010000668         1254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_150308_length_1254_cov_3.214345, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000668 DAOOQX010000000
VERSION     DAOOQX010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150308_length_1254_cov_3.214345"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PLX53_09365"
     CDS             complement(<1..929)
                     /locus_tag="PLX53_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOY73331.1"
                     /translation="MSKDKYPIIEKVRIEDIAAEGKSIARIDGRVLFVPYAVPGDLVD
                     VQLTRKRKNYMEGYVVRYHEYSPIRVEPFCRHFGTCGGCKWQNLPYAEQLKIKQRQVE
                     DQLRRIGRVNFPTVSPILGSERTTGYRNKLEFTFADRRWFSGDELAKADKLPDEPALG
                     YHIPGRFDFVFDVEECFLQPEPSNSVRLAVKNYAADHHIPFMNLRSHEGTLRNLIVRT
                     SSTGDVMVILSLRQLSSDVEQLLDFMVEKFPQVSSFNYVVNAKLNDTINDLEVVCYHG
                     NKYITEEMEGIKFRVGPKSFYQTNSEQACVLYGV"
     gene            995..>1254
                     /locus_tag="PLX53_09370"
     CDS             995..>1254
                     /locus_tag="PLX53_09370"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOY73332.1"
                     /translation="MDSLTHIVLGAVVGEAAVGKKMGNKALVYGAIAGSLPDFDVFVA
                     RFFDPVDALFVHRGFSHSLFLLVILAPLAAYAVKHFSHNPRLT"
BASE COUNT          321 a          315 c          277 g          341 t
ORIGIN      
        1 actccgtaaa gcacacatgc ctgttccgag tttgtctggt aaaacgattt tgggccaacc
       61 ctaaacttta tgccctccat ttcctcagta atgtatttat ttccatggta gcacactacc
      121 tccaaatcgt ttatagtatc gttcaacttg gcgtttacaa cgtagttgaa cgacgagact
      181 tggggaaact tttcaaccat gaagtcgagc agctgctcta cgtcggagct aagctgcctc
      241 aacgacagga taaccattac atcaccagtt gacgaagtac ggacaataag gttacgaagt
      301 gtgccctcgt gcgaacgaag attcatgaac ggaatatggt ggtcagcagc ataattttta
      361 acagcaagcc taacggagtt tgatggttcg ggttgcagga aacactcctc cacgtcgaac
      421 acaaagtcga aacgaccggg aatatggtat cccagcgcag gctcatcggg tagcttgtcg
      481 gctttggcca gctcatcgcc actaaaccag cgtctatcgg caaaggtaaa ttcgagcttg
      541 tttcggtaac cggtagttct ttctgagcct agaatgggcg aaacggttgg aaaattgacc
      601 cttccaattc gtcgaagctg atcctcaacc tgtctttgct ttattttgag ctgctcggca
      661 tagggcaggt tttgccactt gcacccgccg catgttccaa aatgcctgca gaatggttca
      721 acccgaatgg gcgaatactc atggtaacga accacataac cctccatgta gtttttccgc
      781 tttcgggtta gctgaacgtc aaccaaatcg cccggaacag cgtagggaac aaacaatacc
      841 ctaccgtcaa tcctggcaat agatttacct tcggcagcta tatcctcaat tctaaccttt
      901 tcgattatcg ggtacttatc tttactcact ttatcgtgct atatttgaac caacatcaac
      961 attggctgtt agggtgcaaa ggtagcaaat tgttatggac tcactaaccc acatcgtttt
     1021 gggcgctgtt gttggagaag cagcagtagg taaaaagatg ggcaacaagg ctttggttta
     1081 cggggctata gccggaagtt tacccgactt cgacgttttt gtagccagat ttttcgaccc
     1141 tgttgatgcc ctgttcgtgc atcgggggtt tagccactcc ctctttctac ttgtaatcct
     1201 cgccccatta gctgcctacg cagtaaaaca tttctcccat aaccccaggc taac
//
LOCUS       DAOOQX010000669         1254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_150371_length_1254_cov_2.437031, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000669 DAOOQX010000000
VERSION     DAOOQX010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150371_length_1254_cov_2.437031"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1189
                     /locus_tag="PLX53_09375"
     CDS             227..1189
                     /locus_tag="PLX53_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOY73333.1"
                     /translation="MGKISTFKQVVQLAILAVAIVAVLPACKNKGAKVPAETPTSGSI
                     KIEADESFKPIVDAEVSTFTSLYNLAHIQTVYKPERELIADFLNDSVQVIVSSWTPTE
                     EQRKTLLDAQTVVRTTLVAYDALAIVLNKGNNDSLLTYDNVKDIFTGKITDWNQLNTR
                     SKLGKISIIFDNIHSGNIRYFREKFNLPQQLPPNFYAVNSNAEVVNYVAKSPGALGIV
                     SVNWISDTDDSTSFDLGSKVKLAAISQQYFDKDSYYLPVQGSIYQKTYPFIREVNINS
                     RESFYGLGSGFISFVAGEKGQRIILKSGLVPATMPIRIVEVKNE"
BASE COUNT          389 a          258 c          269 g          338 t
ORIGIN      
        1 ttttacatgc ggggttggtg tatatttgcc cgctctaatg ttaaaaagta ttaaatgata
       61 atggtaacaa gttgatagct agcagggtat taactattat ggactgaaat ttgaagatac
      121 ctaaatacta taagttttca agataattac taatattgtg cgttgttaaa gtattgaata
      181 gggcaaagcg aagaatatta gttgttggcc ggaaaaattt aatactatgg gaaaaatttc
      241 aacattcaaa caagtcgtac agctggcgat attagctgta gccattgtgg ctgtgctacc
      301 cgcatgtaaa aacaagggag caaaggtccc tgccgaaact ccgacctctg gtagcataaa
      361 gattgaagca gatgaatcgt tcaagcccat tgtagatgct gaggttagca cattcacctc
      421 gctctataac cttgcccata tccaaaccgt ttacaaaccc gagagggagc ttattgccga
      481 ttttctcaac gattcggtgc aggtaatcgt ttcgtcatgg actccaaccg aggagcaaag
      541 gaaaaccctg cttgatgctc aaactgttgt tcgtactacg ctggttgcct atgatgcgtt
      601 ggcaattgtg ctgaacaagg gaaataacga ctccctttta acctacgata atgttaagga
      661 tatttttacc ggaaaaatca ccgactggaa ccagctgaat acccgttcca aactaggtaa
      721 gattagcatt atattcgaca acatccactc aggaaatatc cggtacttca gggaaaagtt
      781 caaccttcca cagcagctac cccccaactt ctacgctgta aactcgaatg cagaagttgt
      841 aaactacgtg gctaagagtc ctggtgctct cggaatagtt agcgtaaact ggataagcga
      901 taccgatgat tccacaagtt ttgaccttgg cagcaaggta aagctagcgg ccatttcgca
      961 gcagtatttc gataaggatt cctactacct tcccgttcag gggtcaattt accagaaaac
     1021 ctaccccttt atcagggagg ttaacataaa ctcaagggag tcattctacg gcctaggatc
     1081 cggattcata agttttgttg caggtgaaaa gggtcaaaga atcatactaa agtcgggctt
     1141 ggtcccggct accatgccga ttagaattgt tgaagtaaaa aacgagtaac taaaattaac
     1201 agcaatgaac atgagacgta ttaaccgagc attgcttcct gttgccttat taag
//
LOCUS       DAOOQX010000670         1252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_150819_length_1252_cov_2.132832, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000670 DAOOQX010000000
VERSION     DAOOQX010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150819_length_1252_cov_2.132832"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PLX53_09380"
     CDS             complement(<1..829)
                     /locus_tag="PLX53_09380"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOY73334.1"
                     /translation="MKTTQYLIAFGLQLLLLGCGDKANREEVIVTNAEEQAYFAAETS
                     PAESKSVIQESSSDFSGSSNVSAKIIRHGNINIEVKDYLVARQQIDSLANLYEAAVIS
                     ESHQENETQRGNSISIRVPAEKFDLLLGELAKIAHRVDYQQISTEDVTTEYIDVNTRL
                     QNKMKVERNYQRLLSQATSVDDILKIENQLGAVRAEIESLQGRMNYINHQVSKSLITV
                     YVYQKVEYRYRPDEMPSFGERIKQAINAGWKGLVWLVVFIIKIWPIWLAALVVFVLVN
                     "
     gene            complement(999..1241)
                     /locus_tag="PLX53_09385"
     CDS             complement(999..1241)
                     /locus_tag="PLX53_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOY73335.1"
                     /translation="MEVNFELEGEYIPLDGLLKRCSLASSGAEAHLLVDDGKVKLNGK
                     TESRRRAKVHPGDTVQACGHTIHISASNSNRFDEIS"
BASE COUNT          298 a          307 c          264 g          383 t
ORIGIN      
        1 ggttaactag cacgaatacc accagggcag ccagccaaat tggccaaatt ttgataatga
       61 aaacaaccag ccaaaccaaa cccttccagc ctgcattaat ggcttgcttt attctttcac
      121 caaagctggg catctcgtcg ggcctatacc tgtactcaac tttttggtaa acgtaaaccg
      181 ttatcaggct tttgctaacc tggtggttta tgtagttcat acgaccctga agcgattcga
      241 tttcagccct tactgcacct agctgatttt caatttttag aatgtcgtca acactggtag
      301 cctgggacag tagtctttga tagttccttt ccaccttcat cttgttttgc aggcgggtat
      361 tcacatcaat gtactcggtg gttacatctt cagtcgaaat ttgctggtaa tcaaccctgt
      421 gggcaatttt tgccagctct cccagaagta ggtcaaactt ttcggccggt accctaatgc
      481 ttatgctgtt gcccctttgc gtttcgtttt cctgatggct ttccgaaata acagctgctt
      541 cgtacaggtt ggcaagggag tctatctgct gtctggcaac caggtagtcc ttgacttcaa
      601 tgttgatgtt gccgtggcgg ataatcttcg cagaaacatt cgaggaacct gaaaaatcgg
      661 aagaactctc ctgtatgact gatttacttt cagctggcga agtttcagca gcaaagtatg
      721 cctgttcttc agcattggtg actataactt cctccctgtt agctttgtcc ccgcaaccta
      781 gtagtaggag ctgtagcccg aaggcaatga gatattgggt tgttttcatt ctggtaagaa
      841 ttatttatcc taataatttc gatttatgtc aggcaatttt tttgtattgg ttcgtcggca
      901 aataccgata ataccccccg gtagccatgc gaaaaggttt gcacctgctt gtagtaaaca
      961 tctgctgtta tcacgttggt agctgttgta agaagatatc agcttatttc atcaaaacgg
     1021 ttgctattgc tggccgaaat atgaatggta tgcccacatg cctggacggt atcgcccggg
     1081 tggactttag ccctcctgcg cgactcggtt ttcccgttaa gctttacctt tccatcatca
     1141 accagcaggt gagcctccgc tcccgaagaa gccaggctgc atcgctttaa cagcccgtct
     1201 aacggtatgt actcaccttc caactcaaag ttaacttcca taacctatac ct
//
LOCUS       DAOOQX010000671         1250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_151194_length_1250_cov_2.335565, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000671 DAOOQX010000000
VERSION     DAOOQX010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151194_length_1250_cov_2.335565"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLX53_09390"
     CDS             <1..471
                     /locus_tag="PLX53_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOY73336.1"
                     /translation="RQTDLLIAAAKTGRVVNIKKGQFVSPESMKYAAQKVVDSGNSKV
                     ILTDRGTQFGYQDLIVDFRGIPQMRAFGFPVVVDVTHSLQQPNQTSGVTGGRPDLIET
                     IARAAVATGADGIFMETHPNPAVALSDGANMLDLKYMEELMNHLVQIRQVIRNF"
     gene            complement(458..>1250)
                     /locus_tag="PLX53_09395"
     CDS             complement(458..>1250)
                     /locus_tag="PLX53_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOY73337.1"
                     /translation="LYTDGLRLRQVLVNLISNALKFTEKGFVEFGFVNRPEERQIEFF
                     VRDTGIGIPTDKQDIIFERFRQIQEGSTRKYGGTGIGLYISKSVVNLLGGDIWFESTC
                     GVGTTFYFTIPYEDVNVAHSGAKFYRTSSPEYSWEGRNILVAEDTESNYLYISTILAK
                     TKANVIWARNGEEAVNAALRGAVPVDLILMDIQMPVMDGYEAVTAIRRQNAEVPIIAQ
                     TAYALPHDNIRCFEVGCNDYISKPINALLLKQKINELLNAYIKNS"
BASE COUNT          318 a          306 c          311 g          315 t
ORIGIN      
        1 cgccaaaccg acctgctaat agccgcggcg aaaacaggta gggtggtaaa tatcaaaaaa
       61 gggcaattcg tttcacctga atccatgaag tatgcagcgc aaaaagttgt ggattccgga
      121 aatagtaagg taatccttac tgaccggggc acccaatttg gctaccagga tttgatagtt
      181 gactttaggg ggatacccca aatgagagcc tttggatttc ctgttgttgt ggatgtaacc
      241 cattcgctac agcagcctaa ccaaacaagt ggagttacag ggggaagacc cgacctgata
      301 gagactattg cacgggcagc agtagctacc ggtgccgatg gtatttttat ggagacccat
      361 ccaaatcctg ctgttgcact ttcagatggt gctaacatgt tggatttaaa atatatggaa
      421 gaattaatga accatttggt tcaaatcagg caggtaatca ggaattttta atgtaagcgt
      481 ttagtagctc attaatcttt tgcttgagaa gcaaggcatt aataggcttt gaaatgtagt
      541 cgttacatcc aacctcaaag cagcggatgt tatcgtgggg taaggcatag gcagtttggg
      601 cgattatggg gacttcggca ttctgccttc tgatggcggt aaccgcctca tagccgtcca
      661 ttaccggcat ctggatatcc atcaggatga ggtcaaccgg aactgctccc ctcaaagcgg
      721 cattaacagc ctcctcgcca ttcctggccc aaataacgtt tgcctttgtt ttggcaagta
      781 tggttgatat gtacaggtag ttggactccg tatcctcggc cacaaggata ttcctaccct
      841 cccagctgta ctccggcgag ctcgtcctgt agaacttggc ccccgaatgg gctacgttta
      901 cgtcctcgta ggggatggtg aagtagaagg tagttcccac cccgcaggtt gactcaaacc
      961 agatatcccc accgagcagg ttcaccacgc ttttgctgat gtatagccca atgccggttc
     1021 ccccatactt gcgggtcgat ccctcctgaa tttgccggaa gcgctcgaag attatatcct
     1081 gcttgtcggt agggatgcca atacccgtat cccgaacaaa aaactctatt tgccgctcct
     1141 cgggacggtt cacgaagcca aactccacaa aacccttctc cgtgaacttg agggcgttgc
     1201 tgatgaggtt gaccagcacc tgtcggagcc ggagcccatc ggtgtaaaga
//
LOCUS       DAOOQX010000672         1246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_151994_length_1246_cov_2.476071, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000672 DAOOQX010000000
VERSION     DAOOQX010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151994_length_1246_cov_2.476071"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PLX53_09400"
     CDS             complement(<1..384)
                     /locus_tag="PLX53_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73338.1"
                     /translation="MRKLIFATFLVGLGTMVQAQTSWLNTKVSEMSFYTLPFEYESLE
                     PIIDKATVEIHYSKHQRSYFDKLISAAKGTEYYNKTLAEIFEEINSVPAAIRNNAGGY
                     FNHILYWDVLKPGNRSNTSKELLNAI"
     gene            566..970
                     /locus_tag="PLX53_09405"
     CDS             566..970
                     /locus_tag="PLX53_09405"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOY73339.1"
                     /translation="MAKLNENELTANNINLVGLGTEISGDISSNGDLRIDGTLIGNLS
                     VKGKVVIGETGRIKGELVCKNSDISGQVEGKITVQELLSLKASARVNGDLNVGKLAIE
                     PGCKFTGYCNMLSAEQNFRSDSKLDSGTSLGK"
BASE COUNT          391 a          212 c          273 g          370 t
ORIGIN      
        1 aatagcattc aataactctt tactggtgtt actacgattt ccgggtttta gaacatccca
       61 atagaggatg tgattaaagt agccaccagc attgttgcgt atagcagcag gaaccgagtt
      121 aatttcttcg aaaatttcgg ctagggtttt attataatat tccgttcctt tagcagccga
      181 aattaactta tcgaagtagg agcgctgatg cttggagtaa tgaatttcca cagtagcctt
      241 gtcaataatg ggttccagcg attcatattc aaagggcaag gtgtaaaaac tcatctccga
      301 cactttagtg tttagccacg atgtttgggc ttgaaccata gttcccaagc caaccaaaaa
      361 ggtagcaaaa attaactttc tcatagtaat taatttttgg tatgctcgtt aaacatgtgg
      421 gctgaaatta agttcatgag aatggtgtac aattattcaa gccgaaaaaa atgggttaaa
      481 caatagtaaa aatttaacaa tagctttgat aaatttatat atttgctgcc ttaaaggaga
      541 tatgttgaac tcaaaatagt tggaaatggc aaaacttaat gaaaacgaac taaccgccaa
      601 caacattaac ctggttggcc taggaaccga aattagcgga gatatttcca gtaacggcga
      661 tttaaggatt gacggtacac taatcggaaa cctatcggta aaggggaaag tggtaattgg
      721 cgagacaggg aggattaaag gtgagctggt ttgcaagaac tctgatattt ccggacaggt
      781 tgagggtaag attacagtac aggagttgtt gtcgctgaag gcatcagcac gagtaaacgg
      841 cgatttaaac gtagggaagc tggctattga acccggctgc aagtttacag ggtattgcaa
      901 tatgctttca gcggagcaga atttcaggtc agatagcaaa cttgactccg ggacatcttt
      961 aggtaagtag gcctactgaa aaatgggtaa tccttttcat aggatgatag cacaggtggt
     1021 agtaatcacg atagtggtat cggttattat caacctttat tttgtttata tttacaagca
     1081 accggtcggg gttctttcta cgatgcttcc atttttccca gccgttgtaa gtataatttt
     1141 ttacctgcag ctagcgcgat tcttttcaaa ggacagcaaa aactttaacc aaaaatattt
     1201 attgtttagc acagctaagc tttttatcaa tgttgcaatc tttgta
//
LOCUS       DAOOQX010000673         1243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_152611_length_1243_cov_2.638889, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000673 DAOOQX010000000
VERSION     DAOOQX010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152611_length_1243_cov_2.638889"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1223)
                     /locus_tag="PLX53_09410"
     CDS             complement(51..1223)
                     /locus_tag="PLX53_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73340.1"
                     /translation="MRPDFKNIDYKLVAITDANGNYLYNQGIEDIAFVEGKVTHDMLM
                     NASDSSRWVRLRTFSEPLPYQLLTGTNRPQRDLIELNFSRKPVGGFKLVQVDNPVSEK
                     WYIPEPDNEGDTVKLWIISEKISAADTLKLALSYLKTDSLNMLQPRIDTLRFVYYESE
                     EAGNDSRSKRGKKEEVVKPTGFKVETSIKNMAVAIPGVPFEFTLPSPARKVNGALVQI
                     FNQTDSILEPGVKLVADSINPRIYRFNTSWKPNVTYKMIVLPGAFESYTSTLNDTLKL
                     QLTGADPESFGTLTAKFSGVSKAVVAELLTEKDAVVDRKAVVGNQSVTFTFIAPAKYK
                     LRFIDDLNANGTWDSGNYLKRIQPERVIPFTEGKNKGVINVRANWDNEISVSLQKP"
BASE COUNT          308 a          283 c          293 g          359 t
ORIGIN      
        1 ccttatcggt gggaaacatg gcaaaattca tcagtacaga cataggtatt ttatggtttt
       61 tgcaggctaa ccgaaatctc gttatcccag ttggcccgaa cgtttatcac acccttattt
      121 ttcccttcag taaacgggat taccctttca ggctgaatgc gcttcaagta gttgccgcta
      181 tcccaggtac cgtttgcgtt taaatcatca ataaatcgaa gtttatactt tgcaggggct
      241 atgaacgtaa aggtcacgga ttggttgcct acaacggctt tcctatctac cactgcatcc
      301 ttttcggtca gcagctcagc tacaaccgcc ttacttacac cagaaaattt agctgtgagg
      361 gttccaaaac tttcggggtc agctccggta agctgaagct taagggtgtc gttcagggtt
      421 gaggtgtagc tttcaaaagc acccggcaaa acaatcattt tgtaggttac attgggtttc
      481 caggaggtgt tgaaacggta aatcctgggg tttatcgagt ctgcgacaag cttcacgcca
      541 ggctccaaaa tgctgtcggt ttggttaaaa atctgcacta gcgcaccatt cacctttctt
      601 gcaggtgaag gaagggtaaa ctcaaaggga actccgggga tagcaactgc catgttcttg
      661 atggaggttt caaccttgaa tccggttggt tttacaactt cctccttctt tccgcgcttt
      721 gaacgggagt cattcccggc ttcctcggat tcatagtaaa caaacctaag ggtatctatc
      781 cgaggctgaa gcatgttgag ggaatcggtt ttgaggtacg acaaagccag ctttagcgtg
      841 tcagcagccg aaattttctc cgagataatc caaagtttaa cggtgtctcc ctcgttgtcg
      901 ggttcgggaa tataccattt ctcgctaacg gggttgtcaa cttgtaccag cttaaaaccg
      961 ccaacgggtt tgcgggagaa gtttagctca attagatccc gctgagggcg gttggtaccc
     1021 gtaagtagct ggtagggcag tggctcgcta aatgttcgca gccttaccca cctagacgaa
     1081 tcggaagcgt tcattagcat atcgtgtgtt accttaccct cgacaaatgc aatatcctca
     1141 atgccctggt tgtaaaggta gttaccgttg gcatcggtaa tggcaacaag cttgtaatca
     1201 atgtttttaa aatctggtct catattttta attttgagct atc
//
LOCUS       DAOOQX010000674         1241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_152986_length_1241_cov_3.058179, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000674 DAOOQX010000000
VERSION     DAOOQX010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152986_length_1241_cov_3.058179"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..>1241
                     /locus_tag="PLX53_09415"
     CDS             77..>1241
                     /locus_tag="PLX53_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOY73341.1"
                     /translation="MKSKELIAEQLNKRILVLDGAMGTMIQQLGLTEEDYRGAVFANH
                     PVNLKGNNDVLALSRPDVIASLHKQYLEAGADIIETNTFNANAVSQADYALEDWIEQI
                     NLEAAGIARRLADDFTTLRPDKPRFVAGSIGPTNRTASMSPDVNRPGYRAVTFDTLVQ
                     AYSLQIKALIKGGVDLLLVETVFDTLNAKAALFAAASVMEELQTDIPVMISATIADLS
                     GRLLTGQTLEAFVTSVSHYPLLSIGLNCAFGAEQLLPYVKQLAVVTPFRVSVHPNAGL
                     PNQLGGYDQSAEMMAGWVEQMVQAGAVNIVGGCCGTTPKHIERVAAVASKYPPRKTPT
                     LPRFTRLSGLEMLEIRPESNFINIGERTNVAGSKKFEKLIIEEKYGEALAIARE"
BASE COUNT          333 a          289 c          314 g          305 t
ORIGIN      
        1 gtttactatc tttaccggtg aatacagcgg tggaattctc tcttaaaacc ccacaaacgt
       61 aaaggaaagg caattaatga aatcgaagga attgatagct gagcagctta acaaaaggat
      121 actggtgctt gacggggcca tgggaaccat gattcagcag cttggcctta cggaggagga
      181 ttaccgtggc gcagtgtttg ccaaccatcc tgtcaacctg aagggtaata atgatgtgct
      241 tgcgctttct cgtcccgatg ttattgcatc gcttcataag cagtaccttg aagcaggtgc
      301 tgatatcata gaaacaaata ctttcaacgc aaacgcagtt tcgcaagccg attacgcatt
      361 ggaggattgg attgagcaga taaatttgga ggcagccggt attgcacgac gactagccga
      421 cgattttaca acacttagac ctgataaacc caggtttgta gcgggctcaa ttggccctac
      481 taaccgaaca gcatccatgt cgcccgatgt taaccggcct ggttaccgtg cggttacatt
      541 tgatactttg gttcaggcat acagcttgca aatcaaagcg ttgataaaag gtggagtgga
      601 tttgctgcta gttgaaacgg ttttcgatac gcttaacgcc aaggcagcac tgtttgccgc
      661 tgccagtgtc atggaagaac tgcaaaccga catcccggtg atgatttcag caacaatagc
      721 cgatttgagc ggacgtttgc taaccggcca aaccctggaa gcttttgtta cctcggtttc
      781 acactatccg cttttgagca tcgggcttaa ctgtgctttt ggtgccgagc agctgctacc
      841 ctatgtgaag cagcttgctg tggtaacgcc atttagagtt agcgttcacc ctaacgccgg
      901 gcttccaaac cagcttggag gttacgacca atctgccgag atgatggccg gttgggtgga
      961 gcaaatggta caggcaggag cggtgaacat tgtgggcgga tgctgtggta caacgccaaa
     1021 acacatcgag cgggttgccg ccgttgcttc caaataccca cctcgaaaaa cacctacatt
     1081 accgcgcttt acccgcttaa gcggactgga aatgctcgaa attcgacccg aaagtaattt
     1141 tataaatatt ggtgaacgca ctaacgtagc cggctctaaa aaattcgaga agctaattat
     1201 cgaggagaag tacggtgaag cgctagccat tgctcgtgaa c
//
LOCUS       DAOOQX010000675         1240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_153200_length_1240_cov_2.967089, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000675 DAOOQX010000000
VERSION     DAOOQX010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153200_length_1240_cov_2.967089"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>578
                     /locus_tag="PLX53_09420"
     CDS             <1..>578
                     /locus_tag="PLX53_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOY73342.1"
                     /translation="FCSDDAVTVEQLKRIILRAQQLPFSQRVKENQAAWLDDDNLLIN
                     YNNSDFSMSINDLSLKGKHNIYNSMAAGIAGHVLNIRKDVIRQSLSDFQGVEHRLEPV
                     LTIKGVRYINDSKATNVNSAWYALESMDTPVVWIAGGVDKGNDYSELMDLVKLKVKAI
                     VCLGVDNVKLHKAFGNVVPVMVDVLSAEDAVR"
     assembly_gap    579..588
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            712..>1240
                     /locus_tag="PLX53_09425"
     CDS             712..>1240
                     /locus_tag="PLX53_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOY73343.1"
                     /translation="MGTFVDKYFKGDRAIWLVVILLSAFSLLVVYSSTGSLAFRYHGG
                     NTTYFLVKQLVFLVLALSIVFVTHLISYRVYYPLSSLLMLISIPLLLFTLVFGTRLNE
                     ASRWLEIPGLGITFQSSDVAKFALVMYMAKILSQKQKDIKDLKQGFLPLALPVILICG
                     LILPANFSTAALLGLT"
BASE COUNT          288 a          274 c          304 g          364 t
ORIGIN      
        1 ttttttgttc cgacgatgcc gttaccgttg agcagctcaa acgcattatc ctcagagccc
       61 agcagctgcc cttttcccag cgtgttaagg aaaaccaagc tgcatggctc gacgatgata
      121 acctactaat caactataac aatagcgact tcagcatgtc aattaacgat ctttcgctta
      181 agggtaagca taacatctac aactctatgg ccgcaggcat tgccgggcat gtgctgaaca
      241 tccgtaagga tgtaatccgg cagtcgctat ccgattttca gggggtagag catcgccttg
      301 agccggtttt aactattaaa ggagtcaggt acatcaacga ttcaaaggcc acaaacgtta
      361 actcggcttg gtatgctctg gagagtatgg atacgcccgt ggtatggatt gccggcgggg
      421 ttgacaaggg aaacgattac tcagagctaa tggacttggt aaagttgaag gttaaggcaa
      481 tcgtttgcct tggtgttgat aacgtcaagc tgcataaggc ttttgggaat gttgtcccgg
      541 ttatggttga tgtgctgtcg gctgaagatg ccgttagann nnnnnnnngg ccaatccggg
      601 cgacacggtt ctgctttcac ctgcctgtgc cagcttcgat ttgtttgaaa actatgagga
      661 tagagggcgt aaattcaagg atgcagttag aatgctttaa gcattatacc aatggggact
      721 tttgtagata aatactttaa gggcgatagg gcaatatggc tggtggtaat tctgttatcg
      781 gcattctccc tgctggtagt ttacagctca acgggctcgc tggcctttcg ttaccatggc
      841 ggcaacacca cctacttttt ggtaaagcag cttgtttttt tggtgctggc cctatcaatt
      901 gtatttgtta cccatctgat tagctatagg gtttactacc cgctgtcgtc gcttcttatg
      961 cttatttcca ttcccctgtt gctctttacg ctggtatttg gaaccaggtt gaatgaagcc
     1021 tcccgctggc tcgaaatacc tgggctgggc ataacattcc agtcgtcgga tgtggctaag
     1081 tttgccctgg tgatgtacat ggccaaaata ctttctcaga agcaaaagga tataaaggac
     1141 cttaagcagg gatttttacc gcttgcactt cccgtaatcc tgatttgtgg gcttattctt
     1201 cctgccaatt tttccaccgc tgcacttttg gggcttacat
//
LOCUS       DAOOQX010000676         1235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_154241_length_1235_cov_2.746610, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000676 DAOOQX010000000
VERSION     DAOOQX010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154241_length_1235_cov_2.746610"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1235
                     /locus_tag="PLX53_09430"
     CDS             <1..>1235
                     /locus_tag="PLX53_09430"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HOY73344.1"
                     /translation="PFNPRYDDIAVVIIDGEVKASAERGLSKFSHQSNMFEPDSSLGL
                     EFANGTTGLRILNQDQNGNIWFEAYNFSANRWLERAVKYPKNIYRREPAQFRTIPETI
                     FYDIFPEDNNKVVWIACTDGLFRFDEDAGNLKNPIFRVLIRQVSAGPDHILFNGVYYD
                     GNEGITFPTSLPYNEVKSTTLPYKNNSLAFTYSAPFFGQQQKLLYSYMLVGFDKTWSD
                     WQPNQRKEYTNLPFGTYQFNVKAKNLFNIESPAETFQFTIQKPLYLQWWAITGYVVAL
                     LLIIWLSIRINTQLLRNSNIKLQQLVDERTLELSKSEQALIEKNVELQRQKEEILTQR
                     DELEEHNRHINDSIIYAQTIQQAILPDLSQSLGNIFEHFLIYKPKELVSGDFYWISHA
                     GPKNNPNEKIFIAVVDCTG"
BASE COUNT          376 a          298 c          243 g          318 t
ORIGIN      
        1 ccattcaatc caaggtacga cgatattgct gtggttataa ttgatggaga agtaaaagct
       61 tctgccgagc gtggactttc aaaattcagc caccagagta acatgtttga acccgactcc
      121 tccctagggt tggaatttgc caatggaaca accgggttac gaatacttaa ccaggaccaa
      181 aacggtaaca tttggtttga ggcatacaat ttttctgcaa accgatggct tgagcgcgct
      241 gttaaatatc caaagaatat ttaccgcagg gaacctgccc aattcagaac catccccgaa
      301 actattttct acgatatttt tcccgaagat aataataagg ttgtttggat agcttgtacc
      361 gatgggcttt tccgtttcga tgaggacgcc ggaaatttaa aaaatccgat atttagggtt
      421 ctcattaggc aggtttcggc cggtcctgac cacatactat ttaacggggt ttactacgat
      481 gggaatgagg ggataacatt tcctacaagc ctcccctaca acgaagtaaa atccacaaca
      541 cttccataca aaaacaactc cttagcattc acctactctg ctccgttctt cggtcagcag
      601 caaaaactct tatactccta catgctggta ggatttgata aaacatggag tgattggcaa
      661 cccaaccaga gaaaggagta caccaatctg ccctttggca cataccagtt caacgttaag
      721 gcaaaaaacc tctttaacat cgaaagtcct gccgaaacat tccaattcac cattcaaaaa
      781 cctctctacc tccagtggtg ggcaattact ggctatgtag ttgcactttt gctaattatc
      841 tggctatcca tcagaattaa tacccagctg cttagaaact caaacattaa actccagcag
      901 ctggttgacg aaagaaccct tgagctctcg aaatccgaac aggcccttat tgaaaagaac
      961 gtcgagctac aacggcaaaa ggaggaaatc cttacccagc gggatgagct ggaggaacac
     1021 aaccgccaca tcaacgatag cattatatat gcccagacca ttcagcaggc cattcttccc
     1081 gatttatccc aatcgctggg aaatattttt gagcatttcc taatttataa gcctaaggag
     1141 ctggtctcgg gcgattttta ctggatatcc catgctggcc ctaaaaacaa cccaaacgaa
     1201 aaaattttta ttgctgtggt ggactgtacc gggca
//
LOCUS       DAOOQX010000677         1230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_155279_length_1230_cov_3.323404, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000677 DAOOQX010000000
VERSION     DAOOQX010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155279_length_1230_cov_3.323404"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..475
                     /gene="rsfS"
                     /locus_tag="PLX53_09435"
     CDS             62..475
                     /gene="rsfS"
                     /locus_tag="PLX53_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOY73345.1"
                     /translation="MAGKKGNADETLQLLKCIVDAIEEKKGLDIVSIEIGGLQNAVCD
                     YFVICHANSTTQVDAIAGNIEFKTLELQNQRVYRSSGYENAIWIVLDYIDIVVHVFLS
                     EWRNYYKIEELWADGKLCRYSDPDDDFSSMQPATF"
     gene            528..>1230
                     /locus_tag="PLX53_09440"
     CDS             528..>1230
                     /locus_tag="PLX53_09440"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HOY73346.1"
                     /translation="MSNNKNSGNQMPGDRKGQVPKPPRFNVYWIYILIILGVFLIQFF
                     FSGKSPVVTSWQEVKNQMLANDEVSKLVVVRNMGKVEVYLKEDKLTKYKDRLGEGMGA
                     VPKSGPHFYFTIGSVETFEKQLNEAQQGKPDAQIIYPEYTDQHNYWGDILSWVIPILL
                     IVGLWAFFFRRMTKNAPGGAAGPGNIFSVGKSKAQLFDKESNQKIDFKDVAGLEEAKV
                     EVMEIVDFLKNPKKYT"
BASE COUNT          365 a          230 c          287 g          348 t
ORIGIN      
        1 cctgtttttc acggccaatg aaaattgttt taactttgct ggctaaacta atattgaatt
       61 aatggcgggg aaaaaaggaa atgctgacga aacgctacag ctacttaaat gcattgttga
      121 tgccattgag gaaaaaaaag gattggatat tgtttcgatt gaaattggtg ggcttcaaaa
      181 cgctgtttgc gactactttg taatttgcca cgcaaactcc accacgcagg ttgatgctat
      241 tgccgggaat attgaattta aaactttgga acttcaaaac cagcgggttt accgttcatc
      301 aggatatgaa aatgcaatct ggattgtgct cgactacata gatatagtgg ttcatgtttt
      361 cctttcggag tggaggaatt actataaaat tgaggagctt tgggccgacg gtaaactatg
      421 ccgctacagt gaccccgatg acgatttttc cagcatgcaa ccagctactt tttaaacttt
      481 tggttacttt aatggttatc ttaccttcaa accgtaataa atgaattatg agcaacaata
      541 aaaatagtgg gaatcaaatg ccgggcgata gaaaagggca agtgcctaaa ccgccaaggt
      601 ttaatgttta ctggatttac attcttatta ttcttggcgt tttcttaata caattctttt
      661 tctcgggaaa aagtcctgtt gttacctcct ggcaggaggt taagaaccag atgttggcca
      721 acgacgaggt tagcaagctt gttgttgttc gcaacatggg gaaagttgag gtgtacctta
      781 aggaggacaa acttaccaag tacaaggaca ggctgggaga aggaatgggg gctgttccaa
      841 aatcaggtcc tcatttctac ttcaccattg gctcggttga aacatttgag aagcagctta
      901 acgaagctca acagggaaaa cccgatgcgc aaattatcta cccggaatat accgatcagc
      961 ataactactg gggcgatatt ctctcatggg tcattccaat cctgctaatt gtgggtcttt
     1021 gggctttctt tttccgtaga atgaccaaga atgcacccgg aggtgcagcc ggccccggaa
     1081 acattttttc ggtaggcaag tcgaaagccc aacttttcga caaggaatcg aaccaaaaga
     1141 ttgattttaa ggatgtagca ggtcttgaag aggccaaggt tgaggtgatg gagattgtgg
     1201 attttttaaa aaatccaaag aagtacaccg
//
LOCUS       DAOOQX010000678         1229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_155494_length_1229_cov_3.936968, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000678 DAOOQX010000000
VERSION     DAOOQX010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155494_length_1229_cov_3.936968"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1106)
                     /gene="xerD"
                     /locus_tag="PLX53_09445"
     CDS             complement(210..1106)
                     /gene="xerD"
                     /locus_tag="PLX53_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOY73347.1"
                     /translation="MGWSAAIEDYINFLKLEKSLSKNSVAAYHTDMMKLYGYAEIIGV
                     EPESVTGEQIQDFIAHLHDSGLSKRSQARIVSSVRGFYKYLLIEEVVDSDPTELIELP
                     KTGRKLPEVLSVEEIDAMEAAVDLSMPDGHRNKAIIETLYSCGLRVSELVNLRLTDLF
                     FDDGYVRVLGKGNKERLVPIGHKAIADINMYLSQRIMVSITVDAKSGNIVFLNRRGKQ
                     LTREMVFLIIKKYAKAAGITRNISPHTLRHSFATHLVEGGADLRAVQEMLGHESILTT
                     EIYTHLDKEFLRETILKFHPRG"
BASE COUNT          341 a          306 c          227 g          355 t
ORIGIN      
        1 actttttaat atcataaaag caccgttggg gcgaatggag cttgccatcg gcaaccagct
       61 tcttaatcac cttgtcaacc tccttcttat caattccggc agcctcggca atttctccgg
      121 ccttcatcgg ttttccggca gatgataggg ttttaacaat tttttctcca gtatccatag
      181 ctaaatgatt taaggatgtt tgcacaaagt taacctcttg ggtgaaattt caaaatggtt
      241 tcacggagaa attccttgtc taggtgggta tagatttcgg tagtcagaat ggattcatgc
      301 cccagcattt cctgaactgc acgcaaatcg gcacctccct ccaccaagtg ggtggcaaag
      361 gagtgtctta aagtatgcgg gctaatattc ctggttatcc ctgctgcctt ggcatatttt
      421 ttaatgatga gaaaaaccat ttcgcgggtt agctgttttc ccctacgatt tagaaaaacg
      481 atgttcccag acttggcatc aacagtaatg cttaccatta tgcgctgcga caagtacatg
      541 ttaatgtccg ctatagcctt atggccaatg ggtaccagcc tctccttgtt tccctttccc
      601 aatacccgca cgtaaccatc atcaaaaaaa agatcggtaa gccttaggtt taccagttct
      661 gaaacacgta acccacagct gtaaagggtt tcgatgattg ccttgttgcg atgcccatcg
      721 ggcatactca aatcaacggc tgcctccatg gcatcaatct cctcaacgct taaaacttca
      781 ggcagctttc gcccggtttt aggcagctct ataagctcag ttggatcgct gtcaaccacc
      841 tcctcaataa gcaagtattt gtagaagccc cttacacttg aaactatcct ggcctgagag
      901 cgtttactca acccgctatc gtgaaggtgt gcaataaagt cctgaatttg ctctccggta
      961 accgattcgg gctctacccc tataatctct gcataaccat acaacttcat catgtctgta
     1021 tggtaagctg ccaccgaatt tttagagaga gatttttcca gctttaaaaa attaatataa
     1081 tcctcaatag cggcgctcca tcccataaaa ccattctttt acaattcttt caccacaata
     1141 caaccagatt tataagtata acgaaaatac aaatcgccat acccagagtt tgctgcaaat
     1201 aaacataacg aaatggcaat tttacaaag
//
LOCUS       DAOOQX010000679         1229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_155529_length_1229_cov_2.796422, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000679 DAOOQX010000000
VERSION     DAOOQX010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155529_length_1229_cov_2.796422"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /gene="rplD"
                     /locus_tag="PLX53_09450"
     CDS             complement(<1..523)
                     /gene="rplD"
                     /locus_tag="PLX53_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOY73348.1"
                     /translation="MEAKVLSISGQDTGRTVQLDDSIFAIEPNDHAIYLDVKQILANK
                     RQGTHKSKERSEVSGSTRKLKRQKGTGTARAGSIKSPLFRGGGRVFGPVPRDYSFKLN
                     KKVKLLARKSALTYKVRENAFIVVEDFNIETPKTKEFITICKNLNVADKKTIFVLSET
                     NKNIYLSSRNLQQQ"
     gene            complement(523..1143)
                     /gene="rplC"
                     /locus_tag="PLX53_09455"
     CDS             complement(523..1143)
                     /gene="rplC"
                     /locus_tag="PLX53_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOY73349.1"
                     /translation="MPQGLIGRKIGMTSVFVEDGKNVPCTVIEAGPCVVTQVKTVESD
                     GYTAVQLAFDEKKEKQTTAPMMGHFKKANTTPKRKLAEFRDWSGEMQLGDVITVDIFN
                     TDRWVDVTGITKGRGFQGVVKRHGFGGVGGQTHGQHNRQRHPGSMGASSYPSRVFPGK
                     RLAGRMGGDRVKIINLRVLKVIPESNLLLVKGSIPGAKGSYLIIEK"
BASE COUNT          287 a          307 c          250 g          385 t
ORIGIN      
        1 tctgctgttg taaatttctg gacgacaaat atatattttt attcgtttcg ctcaacacga
       61 atatggtttt tttgtcggct acgttgaggt ttttacaaat agtgatgaac tcctttgttt
      121 ttggggtttc gatattaaag tcctccacaa cgataaaagc attctccctt accttatagg
      181 ttagggcgct tttccgggca agcagcttca ctttcttgtt cagcttaaag ctgtagtcgc
      241 gtgggacggg gccaaaaact ctacccccac ctctaaatag gggcgatttg atgctacctg
      301 cacgggcagt tccggttccc ttttgacgtt tcagcttcct ggtgctacca gaaacctcgc
      361 tacgttcttt tgatttgtga gttccctgtc gtttattggc aagaatctgc ttcacatcca
      421 ggtaaatggc atgatcgtta ggctcgatgg caaaaattga atcgtccagc tggacagttc
      481 tgcccgtatc ttgtcctgat atgctaagta cttttgcttc cattacttct caataattaa
      541 gtaagaaccc tttgctcccg gaatagaacc cttaacgagg agtaggttgc tttcagggat
      601 tactttaagc acgcgtaggt tgataatttt aaccctgtcg ccgcccattc tacctgccag
      661 tctcttgcca ggaaacacac gtgacgggta ggatgaagca cccattgaac ctgggtggcg
      721 ttgccggttg tgctggccgt gggtttgccc accaacacca ccaaatccat gacgctttac
      781 cactccttga aatcctctac ccttggtaat tccggttaca tcaacccatc tatcggtatt
      841 aaagatatca actgtaatca catcgcctag ctgcatttcg ccgctccagt ccctgaactc
      901 ggcgagctta cgcttgggtg tagtgtttgc cttcttaaaa tgccccatca ttggggcagt
      961 agtctgcttt tcctttttct cgtcaaaagc tagttgcacc gcagtataac catcagattc
     1021 gacagttttt acctgagtaa caacgcaagg acccgcttca ataacggtgc aggggacatt
     1081 tttcccatcc tcaacgaaaa cggaagtcat tccaatttta cgtccaatta atccttgtgg
     1141 cattgttttt tacaatttac aggttacact catactttaa tttctacttc gactccactt
     1201 ggaagttcga gcttcatcag cgcatcgat
//
LOCUS       DAOOQX010000680         1226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_156115_length_1226_cov_3.717336, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000680 DAOOQX010000000
VERSION     DAOOQX010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156115_length_1226_cov_3.717336"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(283..915)
                     /locus_tag="PLX53_09460"
     CDS             complement(283..915)
                     /locus_tag="PLX53_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73350.1"
                     /translation="MSRKSLILFTLLSLFGMAARGQLVQDLYKIYDPVFGNDDHDSTA
                     ILTPFGVIKPGVSYNLSVGTSYSSFGSGMGLSETYISPRVSYSPNQKLQVVAGISFSR
                     TGTHGLSTPSPTNISTGQALDNPYQAWAYTQYNFNNRFSIYAMGSVSQNQTYFSPYSR
                     TLGSYNSQMYGVGFNYRISSKASIGASFNFVNRNPFGYQLGGYSNPFIPY"
     gene            complement(1022..1192)
                     /locus_tag="PLX53_09465"
     CDS             complement(1022..1192)
                     /locus_tag="PLX53_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73351.1"
                     /translation="MISKDLRVNMRNLNVNSKNGQFEATIQLYVQDKKHLEMLLYRLG
                     AIPGVSKAERLR"
BASE COUNT          321 a          254 c          301 g          350 t
ORIGIN      
        1 gtatcagtac tataaaagtt gtatgtacac aaatgtagtg tacatgttac aactcatcaa
       61 ataggcttct aatttcctgc aaacttttca ggctcgcttg ggtgtaagca tctgtagctt
      121 gaccacgttt atggccatgt agcaaccgga taaaccaata gtggaccagc ttagagcagg
      181 tgttcggaac gactggtctt agaatgatga atacttagga tagtagcaac ctggggaaaa
      241 ttgttttgcc ctatgtttcg gcttctttaa ccgttaattt ttctagtagg gaataaaggg
      301 gttgctatat ccaccgagct ggtagccaaa ggggttccgg ttaacgaagt tgaagctggc
      361 accaatgctt gccttactac ttatgcggta gttaaagcca acaccgtaca tctggctgtt
      421 gtagctccca agcgttcggg aatatgggga gaagtaggtt tgattctggc taaccgagcc
      481 catagcgtaa atgctaaatc ggttgttgaa gttatactga gtgtaggccc atgcctggta
      541 tggattgtca agggcttgac cggtgctgat gttggtgggg gagggtgttg acaacccgtg
      601 cgttcccgtt cggctaaagg aaattccggc tacaacctgc aacttttggt tgggtgagta
      661 gctcacccta ggcgatatgt aggtttcaga tagtcccatc ccgctgccaa acgaagagta
      721 ggaggtgcca actgacaggt tgtagcttac accgggtttt ataactccaa aaggagttaa
      781 aattgctgta gagtcatggt cgtcgttacc gaaaacaggg tcgtagattt tatatagatc
      841 ctgaaccagc tgtccccttg ctgccatccc aaacaggctt agtagggtaa atagaatcaa
      901 cgattttctg ctcatagcct taccaattta tgaacatcca aatatactaa aagtatggga
      961 gagttgaaat gcaaccgctc caaattagct aactttaagc aattgcaact cggcagcttt
     1021 gctaccttag cctttcggcc ttacttaccc ctggtatggc tccaagccgg taaagcagca
     1081 tttcgagatg ctttttatcc tgaacgtaga gctggatggt agcttcaaac tgtccgtttt
     1141 tggagttgac gttgaggttg cgcatgttta ccctcaaatc cttgctgata atctcggata
     1201 tccgagatta tcagcaagga tttgag
//
LOCUS       DAOOQX010000681         1224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_156534_length_1224_cov_3.979470, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000681 DAOOQX010000000
VERSION     DAOOQX010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156534_length_1224_cov_3.979470"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PLX53_09470"
     CDS             complement(<1..774)
                     /locus_tag="PLX53_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOY73352.1"
                     /translation="MEPFLKLVAKDLYNLLGSELSDITLVFPNRRASLFFARYLAEQL
                     SSPTWQPAFTTVNELMYKVAGLRPANNLWLNYKLYQSYTDITGINQPFDDFYFWGNVM
                     LSDFDQVDKYQVDPQKIFANIKDIKDIDRRFDEFDAEHVEAIKKFLNLIDDHGSSAIR
                     SRYSEIWSKLGVIYSQLRERLLQEGTAYEGLAYRMASEEMNKSQLLTLNERFALIGFN
                     ALNACEKTLFNHLKNFNNALFYWDYDKYYMQNQDYVHEAA"
BASE COUNT          320 a          251 c          279 g          374 t
ORIGIN      
        1 agctgcttca tgcacgtagt cctgattctg catgtagtat ttgtcgtaat cccagtaaaa
       61 cagggcgtta ttaaaatttt tcaggtgatt aaatagtgtt ttttcacaag cgttgagcgc
      121 gttgaaccca attagggcaa atcgttcgtt tagggtgagc agttgggatt tgttcatttc
      181 ttcagaggcc atcctatagg ctaagccttc gtatgccgtt ccctcttgca gcaatcgttc
      241 acgtagctgg gagtatatca ctccaagctt cgaccaaatt tcggagtacc gtgatcgaat
      301 agccgagctg ccgtggtcgt caatcaggtt caaaaacttc ttaattgctt caacatgctc
      361 ggcatcaaac tcatcgaatc ggcgatctat atccttgata tccttaatgt tggcaaatat
      421 tttttggggg tcaacctggt acttgtccac ctggtcaaaa tccgacagca tcacgttacc
      481 ccaaaagtag aaatcatcaa aaggttgatt tattccggta atatccgtgt aggattggta
      541 aagtttatag ttaagccaca ggttatttgc ggggcgcagg cctgctactt tgtacatcaa
      601 ctcatttact gtagtaaagg caggctgcca tgtgggcgag cttagctgtt cggccaggta
      661 cctggcaaaa aagagagatg cccgtcggtt cgggaaaacc aacgtaatat cggatagctc
      721 actccccagt aggttgtaaa gatctttggc tacaagtttt agaaacggct ccaatgatgg
      781 gtagttttag tttgacatga agtattatag gctgttgtgc tctattgact tagctatacc
      841 agttattaat ccaacatgct aatgtagtta cgattggtga tataatttgc tagggttgaa
      901 aaagtttttt tgaaaaaatt ttggtttatt ttccacaact cctgaattct tttacccctg
      961 tcttcagccc caactgggcg gaatagctag caaggggcgt aagccctttg tcaacgaggc
     1021 agcatggtgt attaagccct gaaagggcgg aataattttg gcgaatccgc caatgtaccg
     1081 cgggtaggcg aacaagtttt tcttatactt tatcctttat ccttgtcttc agcactttct
     1141 ggggaaaggc attctccgcg ttgctcggaa atgcaggaat cattaaacgt tgcttgtatt
     1201 agtaaaatgg cataaaagcc tgac
//
LOCUS       DAOOQX010000682         1222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_157045_length_1222_cov_2.599829, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000682 DAOOQX010000000
VERSION     DAOOQX010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157045_length_1222_cov_2.599829"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /gene="sucC"
                     /locus_tag="PLX53_09475"
     CDS             complement(<1..215)
                     /gene="sucC"
                     /locus_tag="PLX53_09475"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF020031.2"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HOY73353.1"
                     /translation="MNLHEYQSKALLKKSGVNVPLGSVAFTPDEAVKVAESVIEATGT
                     DVIAVKAQIHAGGRGKGGGVKIAKSTE"
     gene            291..833
                     /locus_tag="PLX53_09480"
     CDS             291..833
                     /locus_tag="PLX53_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOY73354.1"
                     /translation="MAKNRKLSNEELHRKTVDEYKMVEKFPIVVVLDNVRSLNNIGSV
                     FRTSDALMVERVALCGICGKPPHPEIHRTALGAENSVLWEYFDKTEMAIEKLRGEGYR
                     IISFEQTEHSVNLVDYMPMPGVKYALVFGNELRGVGQDIVDLSDECVEIPQFGTKHSF
                     NISITAAIVLWDFFAKLKKH"
     gene            complement(917..>1222)
                     /locus_tag="PLX53_09485"
     CDS             complement(917..>1222)
                     /locus_tag="PLX53_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73355.1"
                     /translation="ILKGQYPAAAEYLGGTDSFNQGLALLLSGKVDAAKNTFAKVETA
                     KGYYGRAIVGARMQEEATTLDNLRTAIGKDASLKARAKDDVEFRNFFQNEAFKAIAE"
BASE COUNT          337 a          266 c          256 g          363 t
ORIGIN      
        1 tcctcggttg atttggcaat ctttacccca cctcccttac ctcttccacc tgcatgtatt
       61 tgggccttaa ctgctataac atctgttcct gtggcttcta tcacactttc agctacctta
      121 acagcttcgt cgggcgtaaa tgccacacta cccaatggta cattaactcc cgatttcttt
      181 agtaatgcct tagactggta ctcgtgtagg ttcatactcg gctttctatt ttgatttaag
      241 ttgttaaata taaaaaaaat gattgagtaa ttttgtcgaa aattcaactt atggctaaaa
      301 acagaaaact ttccaacgaa gagcttcaca ggaaaactgt tgacgagtat aaaatggttg
      361 aaaagtttcc tattgtcgtt gttctggaca atgtcaggag cctaaataat attgggtcgg
      421 tttttagaac ttccgatgcc ttgatggttg agagggtagc cctttgtgga atatgcggaa
      481 aaccaccaca tcccgaaatt catcgtactg ctcttggagc cgaaaattct gttttatggg
      541 agtattttga taaaactgaa atggctattg agaagttgag aggggaggga taccgcatca
      601 tctcttttga gcaaaccgag cattctgtca acttagttga ctatatgccc atgccaggag
      661 tgaaatatgc tttagtattt ggcaatgagc ttcgtggggt agggcaggat attgttgacc
      721 tttcagatga atgcgtagag ataccgcagt ttggcaccaa gcattcgttc aacatttcta
      781 taactgctgc catagttctt tgggacttct tcgctaagct taagaagcat taaggcagag
      841 cggcaaacca acaaccgtag caccaataaa aaaacctgcc aacggcaggt tcttttataa
      901 actttttact gttcagctat tcagcaatag ccttgaaagc ttcgttctgg aagaagttac
      961 gaaattcaac atcatccttt gcacgagcct tcaacgaggc atccttgcca atagcggtgc
     1021 gtaggttatc aagtgtggta gcttcctcct gcatgcgggc acctacaata gcacggccat
     1081 aataaccctt agctgtttca acctttgcaa aagtattctt tgctgcatca acctttccgc
     1141 taagaagcag ggcaagtccc tggttgaacg agtcagttcc gccaaggtat tcagcagcag
     1201 caggatactg acctttaaga at
//
LOCUS       DAOOQX010000683         1218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_157825_length_1218_cov_4.253654, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000683 DAOOQX010000000
VERSION     DAOOQX010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157825_length_1218_cov_4.253654"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PLX53_09490"
     CDS             complement(<1..426)
                     /locus_tag="PLX53_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOY73356.1"
                     /translation="MQNIRQLFLSHVAQTSDAPLMLEVSRAEGVFLFGPSGEKYYDLI
                     SGISVSNVGHANPRVVKAVQDQASAYMHLMVYGEFIQSPQVLLAAKLSSMLPQTLSSV
                     YFVNSGSEAVEGAMKLSKRFTGRTEIISFRNAYHGGTQGA"
     gene            complement(445..1155)
                     /locus_tag="PLX53_09495"
     CDS             complement(445..1155)
                     /locus_tag="PLX53_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOY73357.1"
                     /translation="MNCIQVDDDELSRRVIEDYASKIDFIRLTGSFPNAVDALNFLKN
                     SLEPVNLIFLDIEMPEMSGIDFLEATSQEPQVIIISSKEKYALKAFDYSVTDYLLKPI
                     TFPRFTKAVSKAYDKFNQQRSPYDDDKEIFIKKNNMLIRVKYSDILWIEALENYIVIN
                     TVNERFTIHLTMKSIENQLSSVRFKRIHRSFIVNVGKIESIEDNATVIRTPEGRKIIP
                     IGKSYRDKLLSEINLINK"
BASE COUNT          331 a          255 c          263 g          369 t
ORIGIN      
        1 cgcaccctgt gtgccgccat ggtaggcatt tcggaacgag attatttctg tcctaccagt
       61 aaaccttttg gatagcttca ttgccccctc aacagcttcg cttccagagt taacaaagta
      121 aaccgagcta agcgtctggg gtagcatgga tgaaagttta gcggccagta gtacctgagg
      181 gctctggatg aactcgccgt aaaccatcag gtgcatgtat gccgaggcct gatcctgaac
      241 agctttgact actctggggt tagcgtgtcc aacattgctc accgagattc ccgaaatcaa
      301 atcgtagtat ttttcgccac ttggcccaaa aagaaaaacc ccctcggctc tgcttacctc
      361 aagcatcagg ggtgcatcgg atgtttgggc aacatggcta agaaaaagtt gcctgatgtt
      421 ctgcatataa aaggttacct gctattactt gttgatcaga ttaatttcgc ttaacagttt
      481 atcgcggtat gactttccga tagggattat tttacgtccc tcaggagttc ggatcactgt
      541 agcgttatcc tcaatgcttt caattttgcc aacattcact ataaatgagc gatgaatcct
      601 tttaaatcga accgaagaaa gttgattttc aatggatttc atggttagat gaatggtaaa
      661 ccgctcgtta acggtattaa taacaatgta gttttcaagt gcctctatcc atagtatgtc
      721 gctatacttt acccgaatca acatgttgtt tttcttgatg aagatctcct tgtcatcgtc
      781 gtagggagag cgttgctggt taaacttgtc gtaagccttg ctaacagctt ttgtaaagcg
      841 gggaaatgta atgggtttca ggaggtagtc ggtaacgctg tagtcaaatg ccttaagggc
      901 gtacttttcc ttcgaggaga ttataataac ctgtggttct tgggaggtag cctctaaaaa
      961 gtctatgccg ctcatttcgg gcatctctat gtccagaaaa attaaattga ctggttcaag
     1021 tgaattttta agaaaattaa gggcatcaac tgcgttggga aaagagcccg ttagccgtat
     1081 gaagtcaatt tttgaagcat aatcttcaat aactctgcgc gatagctcat catcatctac
     1141 ctgtatgcag ttcatcttgt tctagttaag ttaacctgaa aaattctttc taaagaacct
     1201 acacaaactt aatgcaat
//
LOCUS       DAOOQX010000684         1210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_159627_length_1210_cov_2.871861, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000684 DAOOQX010000000
VERSION     DAOOQX010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159627_length_1210_cov_2.871861"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PLX53_09500"
     CDS             complement(<1..885)
                     /locus_tag="PLX53_09500"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HOY73358.1"
                     /translation="MTKREKYSWVYWLSILAFVIALQLLVFPDTQGPREFTYSEFIDS
                     VNAGKVERIVFFEDKIIGQLKSGEETGQKSITQAPTAPWRLRIPSIEQQVKRQFVVNR
                     IPNMNEEYLISILTGTNVSFKGHVENNVLRNFFLNWLLPLLIFMALWGFFFKKMGSKN
                     PMLEIGKNKAQIHAEKPKNPITFKDVAGVDEAVDEVRELVDFLKYPEKFTRLGGKLPK
                     GVLLVGPPGTGKTLLAKAVAGEADVPFFSLSGSDFVEMFVGVGAARVRDLFNQAKVHA
                     PCIIFIDEIDAIGKSRAQN"
BASE COUNT          349 a          268 c          213 g          380 t
ORIGIN      
        1 attttgagcc ctgcttttac ctatagcgtc aatttcatca atgaatatta tacaaggagc
       61 atgaactttt gcctggttga acaaatcgcg gactctggcg gcgccaactc caacaaacat
      121 ttcaacaaag tccgaaccac ttaagctaaa aaagggcaca tcggcttcgc cagctacggc
      181 tttagccagg agagttttac cggtccctgg aggaccaaca agcaacaccc ctttaggcaa
      241 cttccctcct aacctggtga atttttcggg gtacttcaga aagtcaacca gctctctaac
      301 ctcatcaact gcctcatcaa cccccgctac atccttgaat gttataggat tttttggctt
      361 ttcggcatgt atttgcgctt tattcttacc aatttcaagc attggatttt tcgatcccat
      421 ctttttgaaa aagaaccccc atagggccat aaaaataagc agcggtagaa gccagttcag
      481 aaaaaagttt cttaaaacgt tattctctac atgcccttta aagctaacat ttgttccggt
      541 gagaatgctt ataaggtatt cttcattcat gttgggtatc ctattcacca caaattgcct
      601 ttttacttgc tgctcaatac tgggaatgcg aagcctccat ggtgcagtag gcgcctgagt
      661 tattgatttt tgacctgttt cctcaccgct tttgagttga ccaattatct tatcctcaaa
      721 aaaaacaatt cgctcaactt tgcctgcatt gacagaatcg ataaattcac tataagtgaa
      781 ttctcttggc ccttgggtat caggaaatac tagcagctgt aaagctatta caaaagctaa
      841 aatgcttagc cagtataccc acgaatattt ttcacgtttt gtcatgttga gttttttttt
      901 tcgcaatatc caaaactgaa ccaattacta atcatgacaa tttttcagca taggaaaaag
      961 ctgtaggttt aattttgaca tctattacag cgttacagga atttctgtac tccattttgt
     1021 catgcttttg tttggggatg aagatttcaa ttgcctcaga gattcttaga aatactataa
     1081 cccctatgat tgggaaaaag taaacctcaa cccgagtttt ctttgggcat tattatatgt
     1141 atatagtata gtgctcgctc gaagagttaa atgacgtgta aaacccattt gccaacccaa
     1201 tcaagggatg
//
LOCUS       DAOOQX010000685         1205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_160664_length_1205_cov_4.646957, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000685 DAOOQX010000000
VERSION     DAOOQX010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160664_length_1205_cov_4.646957"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLX53_09505"
     CDS             complement(<1..258)
                     /locus_tag="PLX53_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HOY73359.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGKEDLITVEEEVELAQRIR
                     KGDQAALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLG"
     gene            675..>1205
                     /locus_tag="PLX53_09510"
     CDS             675..>1205
                     /locus_tag="PLX53_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOY73360.1"
                     /translation="MNLFTPLNSESGKNLSGSPKPVEHVRVLILGSGPAGYTAAIYAA
                     RANLSPVVYEGIEPGGQLTTTTEVENFPGYPDGVTGTQMMEDLKKQAERFGTDVRFGI
                     ATAADLSSMPYRITIDDEKVVETDALIIATGATAKYLGLESENKFKGQGVSACATCDG
                     FFYKGKDVAVVGGGDTA"
BASE COUNT          346 a          267 c          256 g          336 t
ORIGIN      
        1 ccccaaattc ccctcgttta taaggtcggg caggcttaac ccctgatttt gatactgctt
       61 ggctacagaa acaacaaatc gaaggttggc tcgggttaat ttctccagag ctgcctggtc
      121 gcccttcctg attcgctggg ccaactccac ctcttcctca accgtaatga ggtcctcctt
      181 cccaatttcc tgtaagtact tatcgagcga tgcactctcc cgattagtaa ttgattttgt
      241 gatttttaac tgtctcattc gaaaccttct atttattttg caaagttatg gctcttcaac
      301 atgcaattca agtttatgca gcaaaaaata atgactgcga aactacttag ctcaacttct
      361 atataatgct tccctaaaac ggttggcatt ttgaaattgt tacattattc cggccgaata
      421 ttcttaatta ctattaaaaa tttagcttga ttcaattcag gcttaccgaa aatggaggtg
      481 tatttccaaa acaaaaaagc cttaagcacc tttattgcta caacatttct caaggatatt
      541 tgttgtgagt tggctgtgcc ttaatagcta ctgagtagcg atatggcctt ttttacactt
      601 taaaaacatg aaattagcag actaaaagca gtagatttgt aaccatcaat attaaaattt
      661 caaaagaata agctatgaac ctattcactc ctttaaattc cgaaagcgga aaaaatttat
      721 ccggatcacc aaagccggtt gagcatgtta gggtgctaat actaggctcg gggccggcag
      781 gctatacagc agcaatatat gctgcacgag ccaaccttag ccctgtggtt tacgagggta
      841 tcgaacctgg ggggcaacta accaccacca ccgaggttga aaattttcct ggctacccgg
      901 atggggttac cggcacacag atgatggaag atttgaaaaa gcaggccgaa cgctttggaa
      961 ccgacgttcg attcggaata gccacagccg ctgacctttc aagtatgccc taccggataa
     1021 ccatagatga cgagaaagtt gtagaaaccg atgcgcttat catagctaca ggggcaactg
     1081 ccaagtacct aggtctggaa tcggagaata agttcaaggg gcaaggcgtt tcggcatgcg
     1141 ccacatgcga cggatttttc tacaagggaa aagatgttgc cgttgtgggt ggtggtgata
     1201 ctgca
//
LOCUS       DAOOQX010000686         1204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_160977_length_1204_cov_2.583116, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000686 DAOOQX010000000
VERSION     DAOOQX010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160977_length_1204_cov_2.583116"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLX53_09515"
     CDS             complement(<1..220)
                     /locus_tag="PLX53_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOY73361.1"
                     /translation="MKTIINLFEESVTKYPDNACIWEKLTDKYESSSYSEVRKEVYRL
                     AAGFLSLGIGKNDKVALLSEGRKLWITSE"
     gene            749..>1204
                     /locus_tag="PLX53_09520"
     CDS             749..>1204
                     /locus_tag="PLX53_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HOY73362.1"
                     /translation="MIDHGNLLSCNLCPHGCGANRSMVRGICKTPDTPLVSSVVVHRG
                     EEPVLSGEQGVCNVFFAHCNLQCSYCQNHQISRNSITNREWKMNVNEVAEEICGLLNR
                     GISMLGFVSPSHQVVQMLEILELVRREGYSPRIIYNSNGYDSVETLRQLE"
BASE COUNT          338 a          217 c          275 g          374 t
ORIGIN      
        1 gttcgctggt gatccacagc tttctaccct cactgagcag tgctacctta tcgtttttcc
       61 ctattcctag gctaagaaaa cctgctgcca gccggtaaac ctccttcctt acctcagaat
      121 atgaggatga ttcatactta tcggttagct tttcccaaat gcaggcgtta tcggggtatt
      181 tcgttacact ttcctcaaaa agattgatga ttgttttcat ccgaactatg ttttaatgtg
      241 ttaatattta acttgttaca aaattataca tccttttctt tatcatagta gtttaaatcc
      301 aaaattctgt tcatctacat ttatttttct ttttggtgtg ctaaaactac tggttttctg
      361 acagataggc aggtaaaact agtgattcct agggcaaatg tacaccattt agagaatgta
      421 ccaaggggcg tagtattttt ggggtatatt gttcgtgtta caaatggata cacgaaaggg
      481 ttatcttgct tacaaataga taaaattgtt tgatactagc caacaataat tagcctggtt
      541 aaagctgcat taacgagaag gatgccctga gggcttcaac cttgaaatta ttacaatggg
      601 ttgttgggtc aaaaatgtat tgtaataaca acaaagcgga acagcctgta tgttgaacac
      661 aaaacaatca taatgtaagc aaccaggtta ggtgagttga gagacaagaa aaaatgggaa
      721 gtgcgtatat ttgcaaaaat ttatttaggt gattgatcat ggtaatcttt taagttgtaa
      781 tctttgcccg cacggctgtg gggcaaatcg tagtatggtg aggggtattt gtaaaacccc
      841 cgatactcct ctggtttcat cggttgtggt gcatagggga gaggaaccgg tgctttcggg
      901 tgagcaagga gtttgcaacg tattttttgc acactgcaac cttcagtgca gctactgcca
      961 gaaccatcag attagccgga actccattac gaatcgggag tggaagatga atgtaaatga
     1021 ggttgcagag gaaatttgtg ggctgcttaa ccgtggcatt agcatgttgg gttttgtttc
     1081 gccttcgcat caggtggttc agatgttgga gattctggag ctagtcagga gggaaggata
     1141 ctctcccaga attatttaca actcaaacgg ctatgacagc gttgaaaccc tcaggcagct
     1201 tgag
//
LOCUS       DAOOQX010000687         1204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_160980_length_1204_cov_2.536118, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000687 DAOOQX010000000
VERSION     DAOOQX010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160980_length_1204_cov_2.536118"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PLX53_09525"
     CDS             complement(<1..617)
                     /locus_tag="PLX53_09525"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOY73363.1"
                     /translation="MKLNKLLAAAILFPAVVSCQSTNGKIGKVAMKNDTDSISYAIGV
                     MYAQNLKQSQLETVNYQAVARAIQDIYSGDSANVKMNDNQAMAYINEYFRKLDQKIAE
                     KNLAESTKFLDENKTKEGVVVDSTGLQYKIITEGNGPIPTADAVVKVHYKGTLVNGKQ
                     FDSSYENGEPAQFKLNQVIRGWSIGLQKMKVGSKFMLYIPADLAYG"
     gene            complement(895..>1204)
                     /locus_tag="PLX53_09530"
     CDS             complement(895..>1204)
                     /locus_tag="PLX53_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HOY73364.1"
                     /translation="EVQISSLFSITKICTDFILWPAPPNRYNLIYFDAFAPDDQPEMW
                     TQPQFLKLFNSLLEGGILVTFSSKGSVKEALRQSGFTVHRIPGPPGKRHILRACKSAT
                     "
BASE COUNT          334 a          257 c          242 g          371 t
ORIGIN      
        1 ccataggcca aatcggctgg gatgtagagc ataaacttgg aacccacctt cattttctga
       61 aggcctatgc tccagcctct aattacctgg ttaagtttga actgggcggg ttcaccattt
      121 tcgtatgatg aatcaaactg cttcccgtta acaagagtgc ccttgtagtg aactttaacc
      181 acggcatcgg cagtaggaat ggggccattc ccctcggtaa taattttata ctgaagtccg
      241 gtggaatcaa caactacgcc ctcttttgtt ttgttttcat ctagaaattt ggtgctttca
      301 gccaggtttt tctcggcaat tttctggtca agcttcctga agtactcgtt aatgtatgcc
      361 atagcttgat tgtcgttcat cttgacattg gctgagtcgc cactgtaaat atcctgaatg
      421 gcgcgtgcca cagcttgata gttgacggtt tcgagctgac tttgctttaa attctgcgca
      481 tacatcacac caatagcata gctaatacta tcggtatcat tcttcatggc cacctttcca
      541 atcttaccat ttgtactttg gcacgaaaca acggctggaa aaagaattgc cgcagcaagt
      601 aacttgttta atttcatctg attaatttta tttgattagt tgatattcag attgacacaa
      661 aatttatctc atccagataa cttctagcaa actttcacaa ccgcctcggg ttcaacagca
      721 acgcttctct tccactaatc ctagaattgc ctaaataccc aagttttttt tttaaaatta
      781 ctcaacaata atattccctg cgaaggtaat acatatttaa actgaattaa aattttaggg
      841 taaaaatttc ttggtgctgg tttttgaaac gaaaaattgt aacatttaac aatactacgt
      901 ggcagattta caagccctga gtatatgcct tttaccggga ggtccgggaa tacgatgaac
      961 tgtaaagccc gattgcctca gggcctcctt cacgctgccc tttgatgaaa aagtaaccag
     1021 tattccacct tctagcaagg agttaaagag tttaagaaac tgtggttgag tccacatttc
     1081 gggttggtcg tcgggggcaa acgcatcgaa gtatatcagg ttgtaccggt ttggaggtgc
     1141 aggccataaa ataaagtcgg tgcaaatttt agtgatggag aacagggagc taatctgaac
     1201 ctca
//
LOCUS       DAOOQX010000688         1200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_161786_length_1200_cov_3.384279, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000688 DAOOQX010000000
VERSION     DAOOQX010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161786_length_1200_cov_3.384279"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..>1200
                     /locus_tag="PLX53_09535"
     CDS             368..>1200
                     /locus_tag="PLX53_09535"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOY73365.1"
                     /translation="MATLEKIRNRAGVLVAVVIGLALLAFILGDLLSSGGSLFNRAQM
                     EVAKIGGTSIPFEMLQAKIDESENLQKMFSNQLSLDEQTVLRIREQVWQDLVRTYTME
                     DQYKKLGLSIHPDELFDMVQGRNIHPIIQQQFSDPNTGQLNKEFITMFLKNMDQDPRS
                     KTYWLFLEQEIQKDRLFNKYFNLITKGLYVTSVQAQRALEDRTKKVNFDYTLVAYSSI
                     PDSSISVEGKEIKNYYKSHLSEYKQTASRDIEYVVFPIQPSESDRQAAEEWINKMKDE
                     FL"
BASE COUNT          339 a          267 c          255 g          339 t
ORIGIN      
        1 gcattcccgc gctacgttcc gcccccgacc catttgcggg tcaggctttt atgccattta
       61 cttataccag taacgttcaa cgattcccgc ctttccgtgc accgctgaga atacctttcc
      121 cccgctgggg ctgaagacat ggagaaagtt gaagggtaaa ggataaggtt aacttttccc
      181 ggtttggtaa aaggcaaacg atttacgatt ctatccttta tccttgaaca tcttgactca
      241 ctttctctct cccttttagt taacccccat ataaaaaaat tgcttggtgc ttaaagccct
      301 attgctaaaa ttgctatatt cgtggcttga attttagctg attttctata atttaaatat
      361 catttaaatg gctacactcg aaaaaattag aaaccgggca ggtgttcttg ttgcggtggt
      421 gattggttta gcattgctgg cattcatttt gggcgacctg ttaagttcag gcggatcgct
      481 atttaaccgt gctcaaatgg aggtcgctaa aattggtggc acttctatac ctttcgaaat
      541 gctgcaggca aagattgacg agagcgagaa tcttcagaag atgttttcca accagctatc
      601 actcgatgag caaacggtgc tgcgtatcag ggagcaagtt tggcaggatt tggttagaac
      661 atacaccatg gaagaccagt acaaaaagct ggggctctcc attcaccctg acgagctttt
      721 tgatatggtt cagggaagga acatccatcc aataatccag cagcagttta gcgatccaaa
      781 taccggccag ctgaacaagg agtttatcac catgtttctt aaaaacatgg accaggatcc
      841 ccgctccaaa acatactggc tttttcttga gcaggaaatt cagaaggata ggcttttcaa
      901 taagtacttc aaccttatta ctaagggctt atatgttacc tcggttcaag cccaacgcgc
      961 attggaggat agaactaaaa aggtaaattt cgactatacc ctcgtggcct actcttctat
     1021 tcccgattcc tctatttccg ttgagggaaa agaaataaaa aattactaca aatctcacct
     1081 aagcgagtac aagcagaccg cctcaaggga tatcgaatat gtagttttcc ccattcaacc
     1141 atccgaaagc gataggcagg ctgctgagga gtggattaat aagatgaagg atgagtttct
//
LOCUS       DAOOQX010000689         1198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_162222_length_1198_cov_4.185477, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000689 DAOOQX010000000
VERSION     DAOOQX010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162222_length_1198_cov_4.185477"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PLX53_09540"
     CDS             <1..341
                     /locus_tag="PLX53_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOY73366.1"
                     /translation="VLRIPPALAPVNLAIFPLVKKDGLPEMARKIMDDLKYDFTCQYE
                     EKDTIGKRYRRHDAIGTPFCITVDHQTLDDETVTIRYRDSMEQDRVPASKLREIMGEK
                     VSLTNLLKQV"
     gene            complement(400..1014)
                     /locus_tag="PLX53_09545"
     CDS             complement(400..1014)
                     /locus_tag="PLX53_09545"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOY73367.1"
                     /translation="MHIAVAGNIGSGKTTLTSLLAKYYKWKPQYEDVDDNPYLNDFYE
                     DMERWSFNLQIYFLNSRFSQIVSIHRSGEDVIQDRTIYEDAHIFAPNLHQMGLMSSRD
                     FNNYLNLFNLMTSLVKPPDLLIYLRASVPTLVRQIQKRGREYEKNIRLDYLQRLNERY
                     EAWIEGYNLGKLLIIDVDHLDFSEKPEDLRTVINRIDAQIHGLF"
BASE COUNT          293 a          275 c          306 g          324 t
ORIGIN      
        1 ttgttctccg tattccgcct gcccttgccc ctgttaacct cgcaattttc ccgctggtaa
       61 aaaaggatgg actaccagaa atggctcgta aaattatgga tgatctgaag tacgacttta
      121 cctgccagta tgaggagaag gataccattg gcaagcggta ccgccgtcac gacgccattg
      181 gaactccatt ctgcatcacg gttgaccacc aaacgcttga cgacgaaacg gttaccattc
      241 gctatcgcga cagcatggag caggaccgtg tgcctgcgtc aaagctcagg gaaataatgg
      301 gcgagaaggt ttcgcttacc aacctgttga agcaagttta accctatcat ccggagcgag
      361 aggctccgat acgttacttt ttggaagact tgctactttt cagaaaagtc cgtggatttg
      421 cgcatcaatc ctgttgatta ccgtgcgcag gtcttcgggt ttctccgaaa agtcgaggtg
      481 gtcaacatca ataatcagga gttttccgag gttgtagcct tcaatccatg cctcataccg
      541 ctcgttaagc cgctgcaggt agtcgagccg gatattcttc tcgtactccc ttcctctttt
      601 ctggatttgc ctgaccagcg tgggaacaga tgctctaagg tagatgagca ggtcgggagg
      661 cttcaccagc gaggtcatca ggttgaacag gttgaggtag ttgttgaaat cgcgactcga
      721 catgaggccc atctggtgga ggttgggggc aaagatatgg gcatcttcgt aaattgtcct
      781 atcctgaatt acatcctcgc cgctccggtg aatgctcaca atctgggaga aacggctgtt
      841 gaggaagtaa atttgcaggt tgaagctcca ccgctccata tcttcgtaga aatcgttcag
      901 gtaggggtta tcatccacat cctcatactg gggcttccac ttgtagtatt ttgcaagcaa
      961 gctagttagc gtggttttcc ccgaaccaat gttaccggca actgcaatat gcataatctt
     1021 ttgtggcttt tagctggttg tttgattttt taaaggtagc aatttacatt aaatgaatgg
     1081 catgttgtga ctggttgtag ctgtaacttt gacagaagtg gcataaggta gcattaacag
     1141 caaggaggag atggcaaatg gctagctggc cgatgacagc tccctgtgaa tagccagc
//
LOCUS       DAOOQX010000690         1196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_162795_length_1196_cov_2.168273, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000690 DAOOQX010000000
VERSION     DAOOQX010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162795_length_1196_cov_2.168273"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="PLX53_09550"
     CDS             <1..991
                     /locus_tag="PLX53_09550"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOY73368.1"
                     /translation="YSIKVAEKDTKTNLMHRILIYDHSSDEGNNTVTYADSGYMKPTG
                     DQKFLIATLYNGYSYKEVKEKSGSRRNPSTNYPAQKQYFEKQVIVFELKGFDLQRTDE
                     SFFKDSYQVLNLSQLEKAEDSLSTALNLQLESFSQNLTQNSIMRNPNWLKRTGSSPVS
                     YKLDFDSLFSKLSPEQKQMAIARALDNARSTKIFVSNSKDEFKYRQKYISRHKIEWNR
                     KFTLSFACFVFFFIGAPLGAIIRKGGLGMPVVISVLFFIAYYIITITGEKFARELVWS
                     PGLGMWVSSFILLPLGIFLSYKATNDSSLMNADFYTEKLKKILRLGRKAKSNK"
BASE COUNT          358 a          314 c          247 g          277 t
ORIGIN      
        1 ttacagcatc aaggtggctg aaaaggacac caaaactaac cttatgcacc gcatcctcat
       61 atacgaccac agctccgacg agggcaacaa cacggttacc tatgccgact caggctacat
      121 gaagcccaca ggcgatcaga aatttttaat tgccaccctt tacaacgggt attcctacaa
      181 agaggttaag gaaaaatcgg gctcacgaag aaatcccagc accaactacc ctgcccaaaa
      241 acaatatttt gaaaaacagg tgatagtttt tgagctcaag ggatttgacc ttcagcgcac
      301 cgatgagagc tttttcaagg acagctacca ggtgctaaac ctttcacaac tcgaaaaggc
      361 agaggattcg ctctccaccg ccctaaacct gcagcttgaa agtttttccc agaaccttac
      421 ccaaaacagc atcatgcgaa atcctaactg gcttaagaga accggttctt cgccggtatc
      481 ctacaagctg gattttgatt cgctgttcag caagctatca ccggaacaga aacaaatggc
      541 cattgccaga gctctcgaca atgcccgctc caccaaaata ttcgtatcca actcaaagga
      601 tgaattcaag tacaggcaaa agtacatcag ccggcacaag attgaatgga acaggaagtt
      661 cacgctttct tttgcctgct tcgtattctt tttcattgga gctcccctgg gggcgataat
      721 cagaaagggt ggattgggta tgccggtggt aatttcggtt cttttcttta tcgcatacta
      781 cataataacc attaccggag aaaagtttgc ccgagagctg gtctggagtc ccggcctggg
      841 catgtgggtt tcatcattca tactgctacc cttgggaatc tttctatcct acaaggctac
      901 aaacgactct tcgctaatga atgccgattt ctacaccgaa aaactcaaaa agatacttcg
      961 acttggccgg aaggcaaaaa gcaacaagta accacaggca attaacatct ccctcatagg
     1021 agctatattc ataaggcaat gagaatactg gttctagcgc ataaaccgcc attccctcca
     1081 gtggacggtg gaactctggc cacccttaac atgtgcatag gacttgtgaa ggcaggaaac
     1141 acggtaaccg tactcacgct cagcaccccc aagcacccca gctctattga gcgaat
//
LOCUS       DAOOQX010000691         1192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_163683_length_1192_cov_2.034301, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000691 DAOOQX010000000
VERSION     DAOOQX010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163683_length_1192_cov_2.034301"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            384..>1192
                     /locus_tag="PLX53_09555"
     CDS             384..>1192
                     /locus_tag="PLX53_09555"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOY73369.1"
                     /translation="MTKVDVLLGLQWGDEGKGKVVDVLTPNYDAIARFQGGPNAGHSI
                     EFNSIKHVLHTIPSGIFHPSCINIIGNGVVLDPIVFAEEVEILTKMNVDLHKTLLISK
                     KAHLILPSHRELDAASEAAKGKGKIGSTLRVIGPTYMDKTGRNGLRVGDILSPDFTRN
                     YEFLKNKHKELLQQYTYQSNIEQHEDAWFKGVEVIKQFRLVDSEYDINEMLVAGKRVL
                     AEGAQGSLLDIDFGSYPFVTSSNTICAGACTGLGVSPHSIGEVYGIFKAYCT"
BASE COUNT          331 a          284 c          259 g          318 t
ORIGIN      
        1 taatctgtac acgctgcggg aaggttaccg agttttgcga ccccagaatt caggaaataa
       61 tcaactcggc aaaacgaaac tccaacttca ccatctctca ccactcgctt tacatatacg
      121 gcatttgtgc cgaatgtcgt aacgaaatcg aaaaagttcc aatcaccctg taacgctccc
      181 ttgccgggta tgtgtcatca cggcaagcct gggctatggt tgtcttttcc acattactaa
      241 ttccaacagc caaccccatt accagaatcc actccgtatt aaaattggtg ctgtttaaaa
      301 cttttacagc gccatttacc taaattttgt aactttaaga ggttaaattt tatgatcgat
      361 tccataatct tttcatataa gctatgacaa aagttgatgt actgctaggg ttacagtggg
      421 gcgacgaggg gaagggcaag gtagttgatg tgcttacccc taactatgat gcaatagcac
      481 gctttcaggg aggcccaaat gccggccatt caattgagtt caacagcatc aagcatgtgc
      541 tgcacacaat tccctcggga atattccacc cttcatgcat taacatcata gggaacgggg
      601 tggtgctcga ccccattgtt tttgccgagg aggttgaaat tctaactaaa atgaatgttg
      661 acctccataa aaccctgcta atttcaaaaa aggcccacct gatactcccc tcccaccgcg
      721 aattagacgc agcttccgaa gcagctaaag gaaagggtaa aattggctct acccttagag
      781 tcattggccc cacatatatg gacaaaaccg gccgcaatgg ccttagagta ggcgacattc
      841 tttcccctga ttttaccaga aactacgagt ttttaaaaaa taagcataag gaacttctcc
      901 agcagtacac ataccagagc aatatcgagc agcacgagga tgcttggttt aagggcgttg
      961 aggtgataaa acagtttcgg ctggttgact cggagtatga tattaacgaa atgctggtcg
     1021 ctggtaaacg ggtgcttgct gagggcgctc agggttcgct cctcgatatt gattttggtt
     1081 cgtatccatt cgttacctcc tcaaatacca tttgcgccgg cgcatgcacc ggtttaggag
     1141 tttcgcccca tagcattggg gaggtttatg gaatttttaa ggcatactgc ac
//
LOCUS       DAOOQX010000692         1191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_163851_length_1191_cov_2.540493, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000692 DAOOQX010000000
VERSION     DAOOQX010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163851_length_1191_cov_2.540493"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLX53_09560"
     CDS             complement(<1..184)
                     /locus_tag="PLX53_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73370.1"
                     /translation="MDFDFNVQKIEEAYRHELLSYLNQLFTGVNLPSHDISHHERVWR
                     YCRSLLLEINRFGLDVP"
     gene            complement(268..528)
                     /locus_tag="PLX53_09565"
     CDS             complement(268..528)
                     /locus_tag="PLX53_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOY73371.1"
                     /translation="MTKIITFNELRRVKDNLPAGSMQVIADRLNINVETVRNYFGGTN
                     YKDGAAVGFHVEQGPDGGLVTLDDTTILDIAYEILGNPRELV"
     gene            675..>1191
                     /locus_tag="PLX53_09570"
     CDS             675..>1191
                     /locus_tag="PLX53_09570"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOY73372.1"
                     /translation="MSIRLVATDLDGTLMRSNGEFSVEDIGTLCRLGKLGVVRVIATG
                     RSPYSASRVLSPDFPIDYLVFSSGAGILKWDTKTLVHTNEMPAALVQTIISWLCFHGI
                     DFMVHNPIPLNHYFQYFTAGNSNPDFERRLNLYKEFATPLIQGVPYPNPASQVVAILP
                     NDEGMFNSLRSG"
BASE COUNT          338 a          275 c          243 g          335 t
ORIGIN      
        1 cagggacatc aagaccgaac ctgtttattt ccagaagcaa actcctgcag tagcgccaaa
       61 ccctctcatg gtgcgatatg tcatgcgatg gtaaatttac ccccgtaaat agctggttaa
      121 gataggatag cagctcgtgg cggtaagcct cctcaatttt ctgaacgtta aaatcaaaat
      181 ccatttgggt aagaattcca gatgtataaa aaaacaggat atcgccaaag gtatcctgtt
      241 tttacaacga agcaaaaaga atgttgttta caccaactcc ctcgggtttc caagaatttc
      301 gtaggctatg tcgagtattg tggtatcatc aagagtaacg agtcctccat cggggccctg
      361 ctcaacgtga aatccaactg ctgctccatc cttgtagttg gtgccgccaa aataattgcg
      421 gacagtttca acattaatgt tcaacctgtc cgcaataacc tgcatgctgc cggctggaag
      481 gttatcctta accctcctga gctcattaaa tgtgattatt tttgtcataa ttctagcggt
      541 ttagataatg agtttaacgt tttaaatata tgcaattaat cctaaaataa aaattattta
      601 acacatttta ttttgaattt agccagactg caaaaaaccg ctaatcgtcc tatatttggg
      661 aaaaaataaa actcatgagt ataagattag tggctaccga cttggacggg accctcatgc
      721 ggagcaatgg tgaatttagc gttgaagata ttggcacact atgccgatta ggtaagctgg
      781 gtgttgtaag ggtaattgcc accggccgtt cgccttactc cgcatccagg gttctttcgc
      841 ctgatttccc aatagactac ctggttttct catcaggagc tggaatttta aaatgggaca
      901 caaagaccct tgtgcatacc aatgaaatgc cggcggctct agttcagacc attatttcat
      961 ggctttgttt tcatggcatt gattttatgg tgcataaccc cattccgctc aaccactact
     1021 tccagtactt caccgcgggc aacagcaacc ccgattttga aagacgcttg aacctttaca
     1081 aggaattcgc cacgcctcta attcagggag ttccgtaccc caatccggca tcccaggttg
     1141 tagccatact acccaatgac gaaggcatgt tcaacagctt gcgttcagga g
//
LOCUS       DAOOQX010000693         1188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_164562_length_1188_cov_2.352162, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000693 DAOOQX010000000
VERSION     DAOOQX010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164562_length_1188_cov_2.352162"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PLX53_09575"
     CDS             <1..637
                     /locus_tag="PLX53_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73373.1"
                     /translation="HDTYVVTSQVVVAADHPDYVPYIETGMSIYANFDDIFCKFAVDN
                     ADNVGSFSLIANPETGYKNDSKTCLQFTKVNSQWANVYIKLSSAYRFDLTVQTKFKVL
                     VYGNAGDKVLLKLENTDMGGNAWQTATHDLFYTIQESNEWEIAEFNFSGIGAGWDWTG
                     TIFTSDVTTDPNFNTGFYNVVRIMVNPGDATATYSVILDNWAGPTVNGWKK"
     gene            740..>1188
                     /locus_tag="PLX53_09580"
     CDS             740..>1188
                     /locus_tag="PLX53_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOY73374.1"
                     /translation="MLKRLLPILIAVALVGCKNQQLKQTEVGSGKFAVNKQVEAKVDS
                     LLSLMTLEEKIGQLNQLSGNGEVTGPISFPAEYMEALRKGLVGSMLNINGAAYTYKVQ
                     KVAVEESRLHIPLIFGYDVIHGYKTIFPIPLGEAASWDMEAIEKSARV"
BASE COUNT          332 a          244 c          278 g          334 t
ORIGIN      
        1 ccatgacacc tatgtggtta ccagtcaggt tgttgttgct gctgatcacc ccgactatgt
       61 gccctatatt gagacgggca tgtccatcta tgccaatttt gacgatatat tctgcaagtt
      121 tgctgttgat aatgccgaca atgtgggttc tttcagcttg attgctaacc ccgaaacagg
      181 ttacaaaaac gacagtaaga cctgccttca atttaccaag gtcaacagcc aatgggcaaa
      241 tgtttacatt aagctttcca gcgcatacag gttcgattta accgttcaaa ccaagttcaa
      301 agtgctggtt tacggtaatg ctggcgacaa ggtgttgctt aagctcgaga acactgatat
      361 gggaggcaat gcatggcaaa ctgccactca tgatttgttt tacaccattc aggaatctaa
      421 cgagtgggaa attgccgaat ttaacttttc gggcattggc gccggatggg attggacagg
      481 cacaatcttt acaagtgatg ttactacgga tcctaacttt aatacgggct tctacaacgt
      541 tgttcgcatt atggttaacc cgggtgatgc tactgcaacc tatagcgtta ttctggataa
      601 ctgggcaggc ccaacagtaa acgggtggaa aaagtaaatt tttgaaatgc accagaggta
      661 attcctttgt tggaattatc tctggtttgc tccgacaacg cattccactt caatattttt
      721 atacactaaa atgaaaaaga tgcttaaaag attattgcca attcttattg ccgttgccct
      781 tgtaggatgc aaaaatcagc agctgaaaca gacagaagtt ggtagtggca agtttgccgt
      841 taataagcag gttgaggcta aggttgactc cctgcttagc cttatgacgc ttgaggagaa
      901 aatcggacag ctaaatcagc tatctggaaa cggcgaggtt actggcccaa tttctttccc
      961 tgccgaatac atggaagcac tccgaaaggg actggttggt tcgatgttga atatcaacgg
     1021 tgcagcatat acctacaagg tgcaaaaggt tgctgttgag gagagccggt tacatattcc
     1081 tttgattttt ggatacgacg ttatccacgg atacaaaaca attttcccaa ttccgttggg
     1141 cgaggctgca tcatgggata tggaagctat tgaaaagtcg gccagagt
//
LOCUS       DAOOQX010000694         1183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_165757_length_1183_cov_2.378546, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000694 DAOOQX010000000
VERSION     DAOOQX010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165757_length_1183_cov_2.378546"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1183)
                     /locus_tag="PLX53_09585"
     CDS             complement(<1..>1183)
                     /locus_tag="PLX53_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOY73375.1"
                     /translation="VKGDVHDIGKNIVSVVLACMGYEVIDLGVMVPTDKIINGVIGHK
                     ADILGLSGLITPSLEEMANVARELEHSGLTIPLLVGGATTSELHTALKIDTEYSGSVI
                     HVKDASLAGGVVAELLSPNQHEMYTQKVKERYAGLRSTYSEQKKTLISIDDARQNRFK
                     FDWSSYTPPQPNHRGIQPFLNVDISELRPYISWTFFFHAWGLKGRFPEILTDPVTGSE
                     AKKLLDEANRMLDDIAAAKSIRASGVAGLFPAASQNDDILVFTDETRSSVMATLPQLR
                     NQQLKTNGEFNLSLADYLAPLNSGIPDWLGCFVVTSGIGVDELANKYRAAGDDYKAIM
                     VKLLADRLAEAFAEKLHQQVRKELWGYRPNENLSPAEVLAGRYDGIRPAPGYPACPDH
                     RG"
BASE COUNT          298 a          307 c          266 g          312 t
ORIGIN      
        1 cccccggtga tcggggcagg caggataacc aggagccgga cgaataccat catacctacc
       61 agctaaaact tcggcaggtg agaggttttc gttaggtcta taaccccaaa gctcctttcg
      121 tacctgctga tgtagctttt cggcaaaagc ttcggccagc ctgtcggcca acagtttaac
      181 catgatagct ttgtaatcgt cgcctgcagc acggtacttg ttggccagct catctacccc
      241 aattcctgaa gttaccacaa agcaacctag ccagtcgggt atgcccgagt ttagcggtgc
      301 cagatagtca gccagtgaaa ggttgaactc cccatttgtc tttagctgct gatttctaag
      361 ctgtggcagg gtagccatca ccgaacttcg ggtttcgtcg gtaaatacca aaatatcgtc
      421 gttctgcgag gcagcaggga aaagccctgc tacacctgac gctcgaattg attttgctgc
      481 ggctatgtca tccagcattc ggttagcctc atcgagaagc tttttagcct cggaacctgt
      541 aacaggatcg gtaagaattt cgggaaatcg tcccttgagc ccccaggcgt gaaaaaagaa
      601 tgtccagcta atgtagggtc ttaactccga aatatcaaca tttaggaatg gttggattcc
      661 cctatggttg ggttgtggcg gtgtgtagct gctccaatcg aacttaaacc tgttttgacg
      721 ggcatcgtca atagaaatca gtgttttttt ctgctcgcta taagtgcttc ggagtccggc
      781 gtaacgttcc ttaacttttt gagtgtacat ctcatgttga ttcggagata acaactcagc
      841 aaccacgcca ccggctagcg aagcgtcctt tacgtgaata accgaacctg aatactcggt
      901 atcaattttc agggcagtgt gtagttcgga tgttgttgcc ccaccaacaa gtaaggggat
      961 agttaatcca ctatgctcca actctctggc cacatttgcc atttcctcaa gtgatggagt
     1021 tataagcccg ctaagcccca aaatatcggc tttatggcca attaccccat taataatttt
     1081 atcagtcgga accatcacgc ccaggtcgat cacctcatac cccatgcagg ccagcaccac
     1141 actcacaata tttttaccaa tatcatggac atcaccctta acg
//
LOCUS       DAOOQX010000695         1182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_165934_length_1182_cov_2.906832, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000695 DAOOQX010000000
VERSION     DAOOQX010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165934_length_1182_cov_2.906832"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1182
                     /locus_tag="PLX53_09590"
     CDS             <1..>1182
                     /locus_tag="PLX53_09590"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73376.1"
                     /translation="SIQVVSIDTLLPPNGKKSLQLSTFGIKGTNEAYLEASVTPPLNL
                     NKRLGYLIQYPHGCVEQTTSAVFPQLYLSQLVDLTADQQKKTSRFVSEAIKKLAGFQN
                     SDGSFRYWPSYVYGDDWSTSYVGHFLLEARSKGYDVPEGMLSRWTSYQGKSARNYIPR
                     PTNPFNSDLVQAYRLYTLALAAKPEVGAMNKLKEQELGLIAKMRLAGAYALIGQKDVA
                     LAIVGSVPIKFSPYRELSYTYGSDIRDRGIVLETLILLGLDSKAYPIALEISKALSQN
                     DWMSTQETAVALLALSRLSIDKEKGGELNLSYTLAKNSRVISSRRPVAREQLEIRDGS
                     NTLEIQNMTKNNVYASVVLKGVPAPGNETSYSSNLNIMARYRSASGDELNPYNLKKGM
                     DF"
BASE COUNT          338 a          283 c          276 g          285 t
ORIGIN      
        1 tcaattcaag ttgtaagcat tgataccctc ctaccgccaa acggcaagaa aagtttgcag
       61 cttagcactt ttggcataaa aggcacaaat gaagcttacc tcgaggcatc ggttacacca
      121 cctctaaacc taaacaaaag gttgggctac cttatccagt atccccatgg ctgcgtggag
      181 caaacaacct cagcagtatt cccccaactt taccttagcc aactggttga tctgacagcc
      241 gaccagcaga agaaaacctc acgattcgtt tctgaggcta ttaaaaagct ggcagggttt
      301 cagaatagcg atgggagctt tcgctactgg ccatcctatg tttatggtga tgactggagt
      361 accagctatg taggccattt tcttcttgag gctcggagca aagggtatga tgttccagag
      421 ggcatgctat cgagatggac cagctaccag ggaaaatcgg caagaaatta cattccccgg
      481 cctaccaacc cgtttaacag cgatttggtt caggcatacc ggttgtacac ccttgctctc
      541 gcagcaaaac ccgaagttgg cgctatgaat aagcttaagg agcaggaact tggccttatt
      601 gcaaaaatga ggctggccgg cgcctatgcc cttattggcc aaaaggatgt agcgttggcc
      661 atagtaggaa gtgttcccat taaattcagc ccatacaggg agctaagcta cacttatggc
      721 tcggacatta gggacagggg aatagttctg gaaaccttaa ttttgctggg tttggattca
      781 aaagcttatc cgattgccct ggagattagc aaggctctct cgcaaaacga ctggatgagc
      841 actcaggaaa ctgcagtagc attacttgcc ctctccaggt tatccattga caaggaaaag
      901 ggtggcgagc taaatctatc ctatactctt gccaaaaatt ccagggtgat ttcaagtcgc
      961 aggcctgttg cccgcgagca gttggaaata cgcgatggct ctaacactct tgaaattcaa
     1021 aatatgacta aaaacaacgt atatgccagc gttgttctaa aaggagtacc tgcaccaggc
     1081 aatgagacat cgtacagcag caacctcaac atcatggctc ggtaccgttc agcttcaggt
     1141 gatgagctga atccctataa cctcaaaaag ggaatggatt tc
//
LOCUS       DAOOQX010000696         1182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_165943_length_1182_cov_2.799468, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000696 DAOOQX010000000
VERSION     DAOOQX010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165943_length_1182_cov_2.799468"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLX53_09595"
     CDS             complement(<1..533)
                     /locus_tag="PLX53_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOY73377.1"
                     /translation="MKALKRVALPVIVGFLAVLGTAAAPLATDSLARILNQAGQDTAS
                     LNAVFKKLISVDLPNPTQTDSLLNELLSRFKKLNYNDGVNRVLSFRGDLYYQQGNFQQ
                     ALEVEKELASKYGGSKTNMLPLANAYKMIAGCFFNMGIHDSAVVYAEKSISLFKNLND
                     SQNVARSLNLLGGIYWN"
     gene            complement(690..1079)
                     /locus_tag="PLX53_09600"
     CDS             complement(690..1079)
                     /locus_tag="PLX53_09600"
                     /inference="COORDINATES: protein motif:HMM:NF019903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOY73378.1"
                     /translation="MLAQSTLNGREITIELASEMIEKLVKNPARKDLSIDYIQKVVCE
                     YFGLPVDVLQAKTRKREIVQARQIAMFFSKNLTKSSLSTIGAVIGSKDHATVLHACKT
                     INNLMETDKRFKAQIEDIEKRIKYSNQ"
BASE COUNT          307 a          249 c          258 g          368 t
ORIGIN      
        1 atgttccagt aaatacctcc aagaagattg aggcttcgtg caacattttg gctatcgttc
       61 agatttttga aaagtgaaat agatttctcg gcatacacaa cggcgctatc gtgaataccc
      121 atgttaaaaa aacacccggc aatcatcttg taggcgttgg ccagcggcag catgttagtc
      181 tttgacccac cgtacttgct ggccagctct ttttcaactt caagtgcctg ctgaaagtta
      241 ccctgctggt agtatagatc tcccctgaaa gatagcaccc ggtttacgcc atcgttgtag
      301 ttaagcttct taaacctgct aagtaattcg tttaagaggg aatcggtttg agtagggttt
      361 ggcaaatcaa cggaaatcag cttcttaaaa acggcattca atgaagcagt atcttgtccg
      421 gcctggttta aaattcgggc taaactatcg gtagccaaag gagcagctgc agtgcccaac
      481 acagccagaa atcctacaat tacaggtaga gcaacccttt ttaatgcttt catggcaagc
      541 tgaaatcagc tttaaaatta acttaagctt ttctcccggc tggtaaaccc tacaacaatt
      601 tacaaagata gccttatttg gattaaaatg cagctgggtg aattttaatc aagccgatgt
      661 agtatagggc tgcttgttag gttgtttctt tactggttgc tatactttat gcgtttctca
      721 atatcctcaa tctgggcttt gaatcttttg tcggtctcca ttaggttgtt aattgttttg
      781 caggcatgga gaactgttgc atgatcttta ctgccgatca ctgcacctat tgttgagagg
      841 gaggatttgg tgaggttttt ggaaaaaaac atggcaatct ggcgagcctg cacaatttct
      901 cgttttcggg tttttgcctg gagcacatct actggtaaac caaagtactc gcacacaacc
      961 ttttgtatgt agtctatgga caaatccttt ctggcagggt ttttaaccag tttttctatc
     1021 atttctgatg ccagctcaat ggtaatttcc ctgccgttga gggttgactg tgcaagcatg
     1081 gagatgagcg ctccctccag ctcgcggatg ttctaggtaa tattggaggc tatgtattca
     1141 attacctctt cagggataac aatgccgtcg ttataaacct ta
//
LOCUS       DAOOQX010000697         1178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_166905_length_1178_cov_2.886910, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000697 DAOOQX010000000
VERSION     DAOOQX010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166905_length_1178_cov_2.886910"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLX53_09605"
     CDS             complement(<1..381)
                     /locus_tag="PLX53_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOY73379.1"
                     /translation="MFALKLKLLLSCFVAVLALVNPIQKVFVMTSLQDQFSEADTKMV
                     ALKSSLTALLILLFFLLLGEVVFNYVFHVELYAFQITCGVVLFYNGITGLQKGIFIKV
                     DQNISIHDITAVPIAIPMIAGPATI"
     gene            complement(586..>1178)
                     /locus_tag="PLX53_09610"
     CDS             complement(586..>1178)
                     /locus_tag="PLX53_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOY73380.1"
                     /translation="LKTGQPVESSIPVKLKVNGVETRAFIYDSTTAAYTLKLPVGVSY
                     DILPSVKNFYNKYEQVDLTKTKKGTKVPKNFYITPIEVGQAVNIDFIYFETGKSTLKP
                     QSFRSLNALVDFLNEYPNVIVEIGGHTDNTGSAAINEKISNLRAKAVADYVVSMGIPA
                     SRITSKGYSFNKPKASNATATGRAQNRRVEFTIVGI"
BASE COUNT          313 a          243 c          277 g          345 t
ORIGIN      
        1 aatggttgct ggtcctgcaa tcatgggtat ggcaatggga acggccgtta tatcgtgtat
       61 gctgatgttc tggtcaacct ttataaaaat gcccttttgc aatccggtaa taccattgta
      121 aaacaaaacc acgccgcagg ttatctggaa ggcgtacagc tcgacatgga aaacgtagtt
      181 gaaaacaacc tctcctagca ggaggaagaa caacagaatg agcaatgctg ttaggctcga
      241 cttaagagca accattttgg tatcggcttc gctgaactga tcctgaaggc ttgtcattac
      301 gaataccttt tggatggggt taaccagcgc cagcacggct acaaaacagc ttagcagcaa
      361 ttttagcttt agggcaaaca tgtttcaact tctgtgtgaa aaactactac cggtagtcaa
      421 caggttggcg taaaaatgcc ttttggtttc attgcgggag atgaataggc agtaaaaggt
      481 tgacacagcc aacattattg attggtaccg ggtattgctt taaacaataa aaagaacgag
      541 tatgttctta cacatgacca cactcgttcg tttcatcaga ttgtattata ttcctacaat
      601 ggtaaactca acccttctgt tctgagccct gccggttgct gttgcgttgc tggctttagg
      661 tttgttgaag ctataacctt ttgaagttat ccgcgaggcg gggatgccca tcgataccac
      721 atagtcagct acagcctttg ccctaaggtt ggatattttt tcgtttatag ctgccgaacc
      781 cgtgttgtcc gtgtgcccgc caatttcaac tattacattg gggtactcgt tgaggaaatc
      841 caccagggca ttaagcgatc ggaacgactg gggtttcagg gttgatttac ccgtttcgaa
      901 gtagataaag tcaatgttaa ccgcctgtcc aacctcaata ggtgtaatgt agaagttctt
      961 gggaaccttg gtgccttttt tagtcttggt caggtcaacc tgctcgtact tgttgtaaaa
     1021 gttttttacc gatggaagaa tgtcgtatga aacgcctaca ggaagtttca aggtgtaggc
     1081 agcagtggta gaatcgtaaa taaaggccct ggtttcaact ccattcacct tgagttttac
     1141 aggaatactc gattcaactg gttgtccggt ttttagat
//
LOCUS       DAOOQX010000698         1178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_166927_length_1178_cov_2.637578, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000698 DAOOQX010000000
VERSION     DAOOQX010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166927_length_1178_cov_2.637578"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLX53_09615"
     CDS             complement(<1..286)
                     /locus_tag="PLX53_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOY73381.1"
                     /translation="MSNPNFIDYVKIYCRSGQGGAGSVHFRREKYIPKGGPDGGDGGR
                     GGSIYIRGNQQLWTLLHLKYKRHIFAGDGESGSGARCTGKDGKDEIIEVPV"
     gene            complement(326..901)
                     /locus_tag="PLX53_09620"
     CDS             complement(326..901)
                     /locus_tag="PLX53_09620"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOY73382.1"
                     /translation="MLNIILFGPPGAGKGTQSQKIIEKYNLQHLSTGDMLRSAIQQNS
                     PLGLKAKEFMDKGQLVPDEMVIDLIASRIDANGSTKGFVFDGFPRTTLQAAKLDRMLE
                     QRKTPITLLISLEVDYDELIARLVNRGKVCDRTDDQDENVIRGRIDVYNKTTLPVLDY
                     YKAQGKLRSVSGMGSIDEIFERICHIIDCVV"
BASE COUNT          308 a          304 c          243 g          323 t
ORIGIN      
        1 caaccggaac ttcaataatt tcatcctttc cgtccttacc agtgcatctt gcccctgacc
       61 ctgattcgcc atcacctgca aaaatatgtc gtttatattt cagatgaagt aacgtccaca
      121 gctgctggtt tccccttatg tagatggagc caccccttcc gccatcacct ccatcagggc
      181 cacccttagg aatatacttc tcgcggcgga agtgaacgct tccagcgccg ccctgacccg
      241 agcggcaata gatttttacg tagtcaataa agttgggatt ggacatcttg caacaggtat
      301 aatgtgtggt tgcggaagca ttatgctaaa caacgcagtc aataatatga cagattcgct
      361 caaaaatttc atctatagag cccatgccgc tcacgctacg cagtttcccc tgagccttgt
      421 agtaatccag aactggcagg gttgtcttat tgtaaacgtc aattctaccg cgaatgacat
      481 tttcgtcctg gtcgtcggtt cggtcgcaaa ctttacccct gttaaccagc cgggctatga
      541 gctcatcgta gtccacctca agggaaatta acagggtgat tggcgttttt ctctgttcaa
      601 gcatacggtc gagcttggct gcctgaaggg ttgtccgagg aaaaccatcg aatacaaaac
      661 ccttggtgct accattagcg tcaatcctgg aggcaatcag gtcaataacc atctcatcgg
      721 gtacaagctg gcctttatcc ataaactcct tggcctttaa acctaaaggg ctgttctgct
      781 gaatggccga tctcagcata tcgccagttg ataaatgttg aaggttatac ttttcaatta
      841 tcttctgcga ttgagttcct ttcccggccc cgggaggtcc gaacaagatg atgttaagca
      901 tggcggtatg ttagcattaa ggttaaaaat caaggtaaaa ggataaaggt taaaggataa
      961 aagataataa acctatctgc ctcctgacgg aacttgtccg gctcctgacg gattcaaaaa
     1021 atctcggctg taatagattc aaatgattca tccccaacca tttaacactt agctggccca
     1081 agtttaacag catatttccc cttccctttc ccaataactt tcacccttca gggcttacgg
     1141 ctcgctgttc gagcgtccca acattaactt tcaggcca
//
LOCUS       DAOOQX010000699         1174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_167911_length_1174_cov_2.243968, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000699 DAOOQX010000000
VERSION     DAOOQX010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167911_length_1174_cov_2.243968"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..>1174
                     /locus_tag="PLX53_09625"
     CDS             68..>1174
                     /locus_tag="PLX53_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73383.1"
                     /translation="MRVRSCLFTALLVTLICVGARAQAPNGISYQAVLRDADGKALAN
                     QAATLRLSLTSSDGTTVYFSETHSVQTDALGMMKVVLGGGQVETGSFAGVPWSSGGPI
                     YLKVEVKLDGQPSYTPMGSEQLQSVPYALASADGVGGSGTKGTLAIWDGAKTLADLPN
                     MTFSNSLSVVGSPTANPDDPIFEVKNSNGDIIFGVYQEGVRINIKDDPIGKGVKGGFA
                     VGGLTNQTKAGPVEYLRITPDSARIYVKQETGTKGLKGGFAVGGLTNQVKGVVSQNLL
                     FLAPDSARIYVKEDGTKGVKGGFAVGGLNNQTKENSAQLIHLNKENYLIGYEAGSSIT
                     TGKYNSFMGYNTGKNTTWGDDNIFIGYQAGFSNTT"
BASE COUNT          302 a          289 c          361 g          222 t
ORIGIN      
        1 ggtggtgaag ctggtgaagg agtagcagca atgcagaata ttgagtaaaa aaattaaagc
       61 aaaagccatg agagtgagga gttgcctttt tacagcattg ctggtgactc tgatatgtgt
      121 tggggcaaga gcccaggcgc ctaacggcat cagctaccag gccgtgctgc gggatgcaga
      181 cggaaaagcg ttggccaacc aggctgctac gctcaggcta agcctgacaa gcagcgatgg
      241 taccacggtt tactttagcg agacccacag cgtgcagacc gatgcgctgg ggatgatgaa
      301 ggttgtgctt ggaggcggac aggtggagac gggaagcttc gccggggttc cctggtcgtc
      361 gggcgggcca atttacctta aagtagaggt aaagctcgat gggcaaccct cctacacccc
      421 gatggggagc gagcagctgc agtcggtgcc ctacgcctta gcttcggccg atggagttgg
      481 tggtagcgga accaagggaa cattggccat ttgggatggt gcaaaaacac tagccgacct
      541 gcccaacatg actttcagca acagcctgtc ggtggtgggt agccccacgg ccaaccccga
      601 cgacccgata ttcgaggtga agaacagcaa cggggatata atttttggcg tgtaccagga
      661 gggtgtccgc atcaacatca aggacgaccc tataggtaag ggtgtaaagg gcggctttgc
      721 cgtgggcggc ctcaccaacc agaccaaggc ggggccggtg gagtacctgc gcatcacgcc
      781 cgatagcgcc cgcatctacg tgaagcagga gacaggaacc aagggattga aggggggatt
      841 tgccgtgggc ggcctcacca accaggttaa gggggtggta tcccagaacc tgctgttcct
      901 tgcacccgac tctgcccgca tctacgtgaa ggaggacggc acaaagggcg ttaagggcgg
      961 ctttgccgtg ggcggcctca acaaccagac caaggaaaat tctgcacagc ttattcacct
     1021 gaataaggaa aactacctca taggctacga ggccggtagt tctattacaa ccggaaaata
     1081 caactctttc atgggataca acacggggaa aaacactaca tggggtgatg ataatatttt
     1141 tatagggtac caggccggct tttcaaacac aacc
//
LOCUS       DAOOQX010000700         1173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_168199_length_1173_cov_1.991950, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000700 DAOOQX010000000
VERSION     DAOOQX010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168199_length_1173_cov_1.991950"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="PLX53_09630"
     CDS             complement(<1..1077)
                     /locus_tag="PLX53_09630"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOY73384.1"
                     /translation="MAGSDFYTYFADRTMRIDYRHSGDDKTESFEMAKIASEGSWAGR
                     TNHLNDPWKLGLYFFEVKDAASGKTIYSQGFCSVFGEWQTTAEAKAGVKTFNETIRLP
                     WPKNEVNVVLQKRDSLNRLVPVWNLTVNPVEYEDLIPTRPSAFEPKVLLDNGDPKFKA
                     DIVILGDGYAENEDSLFWKDAQRFSDAFFSVEPFKSRIADFNVRAVLGKSTQSGIRLQ
                     RKDFYPDNLLKTSYYAFGIERYVLASDDWIVKDYASIVPYDFIVILMNSDKYGGGGIF
                     NLYITAAAHSRASEYVMVHEMGHHIAGLADEYYTSDVAYEPANTKVEPWEFNITALIN
                     PDSLKWRDLVAPGTPIPTPWNKVEY"
     gene            1079..1173
                     /locus_tag="PLX53_09635"
                     /pseudo
     CDS             1079..1173
                     /locus_tag="PLX53_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583754.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
BASE COUNT          306 a          285 c          274 g          308 t
ORIGIN      
        1 atactccacc ttgttccatg gagttggtat aggtgtacca ggggctacga gatccctcca
       61 cttcaggctg tcgggattta taagggctgt tatattaaac tcccagggtt caaccttggt
      121 gttggcaggc tcgtaagcca cgtcgctggt gtagtactcg tcggctaggc cggctatgtg
      181 gtgacccatc tcatgtacca tcacgtattc ggatgctctg gagtgggcag cggcggtaat
      241 atacaggttg aaaatgcccc caccaccata cttatcagaa ttcatgagaa ttactataaa
      301 atcgtaggga acaatggagg cgtagtcctt aacaatccaa tcgtcggatg cgagcacgta
      361 gcgctcaatt ccaaaagcat aatagctggt ttttagcagg ttatcgggat aaaaatcctt
      421 acgttgaagc ctaatcccgc tttgcgttga ctttccaagc actgccctga cattaaaatc
      481 agcaattctc gatttaaaag gttcaacgct aaaaaatgcg tcggaaaacc gctgtgcatc
      541 cttccaaaaa agggagtcct cattttcagc atagccatcg ccaaggatga caatgtcggc
      601 tttaaatttt ggatcgccat tatccagcaa aactttaggt tcaaaagcag atggccttgt
      661 gggtatcaga tcttcgtatt caaccggatt aacagttaag ttccaaacag gcacaagccg
      721 gttaagcgaa tcccttttct gcagcaccac gttaacctca ttttttggcc agggcaaccg
      781 aatggtttcg ttaaaggttt taacaccggc cttggcttcg gcagttgtct gccactcgcc
      841 aaaaacgctg cagaaaccct gcgagtagat agttttgcca ctggcagcat cttttacctc
      901 gaaaaagtaa agtccaagct tccatgggtc gttaagatgg ttggttctgc ctgcccagct
      961 accttcagaa gcaattttgg ccatttcgaa actttcggtt ttgtcatcgc ctgaatggcg
     1021 gtagtcaatc cgcatggttc ggtcggcaaa gtaggtgtaa aaatcactcc cggccacggg
     1081 ctacggacct tatggggttg ccatcctagt tgagaccgca tcctacacga acggtagcga
     1141 atatccgaag ctacttcaac aagcatggag gct
//
LOCUS       DAOOQX010000701         1168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_169383_length_1168_cov_1.947889, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000701 DAOOQX010000000
VERSION     DAOOQX010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169383_length_1168_cov_1.947889"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PLX53_09640"
     CDS             complement(<1..300)
                     /locus_tag="PLX53_09640"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOY73385.1"
                     /translation="MEWWIYLLVVLAGVAAGFINTLAGSGSLLTLPLLIFIGLPANVA
                     NGTNRIGILMQSMVAVGRFKQKKVFEWSDGLWLTIPAGLGAVLGALVAVDLNEVLM"
     gene            complement(324..806)
                     /locus_tag="PLX53_09645"
     CDS             complement(324..806)
                     /locus_tag="PLX53_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73386.1"
                     /translation="MELFHIKLNEFPYRKSKFFRLLIVAAIILALLAILTILIFVNRQ
                     EKGFVGIILIWVSYFLVYLYWAWYSFRVKLYIHADEFAIKFKFGMVPSLSQVILWETI
                     EKVRLGPTYIAFYKKTGRRKMAKLGWLPYSKVVEIKDKVANACRIKHIPVEVADFTHY
                     "
     gene            complement(965..>1168)
                     /gene="glyA"
                     /locus_tag="PLX53_09650"
     CDS             complement(965..>1168)
                     /gene="glyA"
                     /locus_tag="PLX53_09650"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157671.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOY73387.1"
                     /translation="DSRSPFQTSGIRVGTPAITTRGLKEQHMNHIVDLMDRVISSADD
                     EKTITAVKEEVNKFMRDFPLYAW"
BASE COUNT          375 a          281 c          219 g          293 t
ORIGIN      
        1 cattagaacc tcattaaggt caacggcaac cagtgcgccc agcacagcgc ccagacctgc
       61 cggaatagtc agccaaagac catcggacca ctcaaaaacc tttttttgct taaacctacc
      121 aaccgcaacc atgctctgca taagaattcc aatacggttg gttccgttgg ctacatttgc
      181 aggtaaacca atgaaaatca ataaaggtaa ggtaagtagc gaaccactac cggcaagtgt
      241 gttgataaaa cctgctgcaa ctcctgccag aacaactaat aggtatatcc accactccat
      301 atcaaataaa caaatcaaaa gtatcagtaa tgagtaaaat cggccacctc aacgggaatg
      361 tgctttattc tgcatgcgtt ggcaaccttg tccttaattt caaccacctt agagtagggc
      421 agccagccta acttagccat ttttcgccta ccggtcttct tgtaaaaggc aatataggta
      481 gggcccaacc taaccttctc aatggtttcc cataaaatta cctgcgacaa gctgggaacc
      541 atgccaaact tgaacttaat ggcaaattca tcggcatgga tgtagagctt taccctgaac
      601 gaataccatg cccagtagag gtaaaccaag aaataggaaa cccagattaa gattatgcct
      661 acaaatccct tttcttgccg attaacaaaa atcaaaatgg ttaaaatagc gagaagggct
      721 aaaataatag ccgccacaat tagcaacctg aaaaatttcg atttcctgta gggaaactcg
      781 tttagcttaa tgtggaacaa ttccatcttt ttgttagcta ggagtggtag acatgtatta
      841 gcttggtaag ctaatcgccg ttataataat ggaaccaaac ctgcaggctc ggttccactt
      901 ttatacacca cgtgcattaa atccaggttc atactaacaa gagtgcaaaa tacgtaaaac
      961 aagcctacca tgcataaagt ggaaagtccc tcataaactt gttaacctcc tccttaacgg
     1021 cagtaattgt tttttcatca tcagcacttg agataaccct atccatcaaa tcgacaatat
     1081 ggttcatgtg ctgctccttc agcccacggg tagtaattgc aggtgtaccg accctgatac
     1141 cggaagtttg gaatggcgaa cggctgtc
//
LOCUS       DAOOQX010000702         1167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_169531_length_1167_cov_2.654676, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000702 DAOOQX010000000
VERSION     DAOOQX010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169531_length_1167_cov_2.654676"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PLX53_09655"
     CDS             complement(<1..968)
                     /locus_tag="PLX53_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOY73388.1"
                     /translation="MAYKSLHHFINKLEEQNELIRISAFVDPVLEIAEITDRMSKQPN
                     GGKALLFENTGTSFPVLTNALGSINRICLALGVSGLDDVGSDINKLFAELAGPKTNIW
                     EKLGTIPTLGKLSAWLPAQISSRGACQEVVHAEPNINLLPVMQCWPFDGGRFITLPMV
                     NTKDPFTGIRNVGMYRMQVFDPKHTGMHWHQHKVGARHFEAYKKLGKRMPVAVALGGD
                     PVYTYAATAPMPDNMDEYLLAGFLRKKKVKLVKCLTVDLEVPEDVDFVLEGFVDPTEE
                     LAWEGPFGDHTGFYSLADWYPRFHLTCITHRRDAVYPATLVGVPPM"
     gene            complement(1050..>1167)
                     /locus_tag="PLX53_09660"
     CDS             complement(1050..>1167)
                     /locus_tag="PLX53_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73389.1"
                     /translation="GSASRSDRIAKYNQLLRIEEMLGPSAIYPGRNFKYIKK"
BASE COUNT          320 a          277 c          278 g          292 t
ORIGIN      
        1 tccatgggag gcacaccaac cagggtagca ggataaaccg catccctgcg atgggtaatg
       61 caggttaagt gaaaacgggg gtaccagtcg gcaagagagt agaaaccggt atgatcgccg
      121 aatggcccct cccatgcaag ttcttccgtt ggatcgacaa atccctccag tacaaaatcc
      181 acatcctcag ggacttctaa atctaccgtc aggcacttga ccagcttaac cttttttttt
      241 cgtagaaaac ccgcaagtaa gtactcatcc atgttatcag gcattggggc cgtggcagca
      301 taggtataaa caggatctcc accaagcgca acggcaacag gcatgcgctt gcctaatttt
      361 ttgtaggctt caaaatgtcg agctccaact ttatgctgat gccaatgcat ccctgtatgt
      421 tttgggtcga aaacctgcat gcggtacatt cccacgttac gaataccggt gaaggggtct
      481 tttgtattta ccatgggtag ggtgatgaaa cgcccaccgt caaaaggcca gcactgcata
      541 acgggtagca ggttgatatt aggctcggcg tggacaacct cctggcaagc acccctcgag
      601 ctgatttgag cgggaagcca agccgagagc ttacctagcg ttggaattgt gccaagcttc
      661 tcccaaatgt ttgttttggg cccggccaac tcggcaaaga gtttattaat atcggagcca
      721 acatcgtcga gcccagaaac gcccaaggcc aaacaaattc ggtttataga acccaaagca
      781 ttggtaagca ctggaaaaga tgtccctgta ttctcgaaca ggagcgcttt acccccgttg
      841 ggttgcttgg acatcctgtc ggtaatctcg gctatttcca gcacagggtc aacaaaagct
      901 gaaattctta tcagctcgtt ttgctcctcg agcttattga taaagtggtg taacgattta
      961 taggccatat caaaattttg ccaataaatt taaaaaaagg ggttggatgt taatccgaac
     1021 ccctcgattt aatttttaga aagcaaccgt tactttttaa tgtacttaaa gttacgaccg
     1081 gggtaaatag cagaaggacc cagcatttct tcaatacgaa ggagttggtt gtacttggca
     1141 attctatcgg agcgtgatgc ggagcca
//
LOCUS       DAOOQX010000703         1166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_169762_length_1166_cov_2.902790, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000703 DAOOQX010000000
VERSION     DAOOQX010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169762_length_1166_cov_2.902790"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1166
                     /locus_tag="PLX53_09665"
     CDS             <1..>1166
                     /locus_tag="PLX53_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOY73390.1"
                     /translation="DEIREYLILSLRKYRQEKRRGKIVTFDSDWMVDESNIVSLADGS
                     FGGKGRGLSFINTLLYTFDVSQYTPNINLHTPRTSIIGTNEFENFMANNSLYEVVFSA
                     KSYDEIKRYFLKAELSNQLKIRLDRLLQIYYRPLAIRSSGMLEDSIMQPFAGIFDTYM
                     IPNAHADRSIRLQSLMNAIKLVYASVFSPTARAYVKAINFKIEDEKMAVIVQEVVGNK
                     FENYYYPHISGVAQSYNYYPFGHINPEDGFANIAVGLGKHVVEGGKSYRFCPRYPTLI
                     NYTLEDLVKNSQTDFLAIDLSRPDFDLLSGDEAALARLDMSYAEQHGNLKHCASVYSS
                     NNNSLIAGIGQPGPRVINFPNILKYNYIPLSQTIDVILDIVKEAFGSPCEIEFA"
BASE COUNT          316 a          279 c          262 g          309 t
ORIGIN      
        1 ccgatgaaat tcgcgagtac cttattttgt ccctcaggaa gtacaggcag gaaaagcgta
       61 gagggaagat agttaccttt gatagcgatt ggatggttga tgaaagcaac attgttagcc
      121 tggccgatgg ttctttcggg ggaaagggga ggggattgtc gtttatcaac accctgctct
      181 acacgtttga tgtttcgcag tacaccccca acatcaacct gcacacccca agaacatcca
      241 ttatcggcac aaacgagttt gagaacttca tggctaacaa tagcctttac gaggtggttt
      301 tcagcgctaa aagctacgat gagattaaac ggtattttct gaaggcggag ctatcaaatc
      361 aactgaaaat caggctggac aggttgctgc agatttacta taggccactt gccatccgat
      421 cttcaggtat gttggaagac tccattatgc agccttttgc gggtattttt gacacctaca
      481 tgattcctaa tgcccatgcc gaccgctcaa tccgacttca aagcctgatg aacgccataa
      541 agctggttta cgcctccgtt ttctcaccca cagcccgtgc ctatgttaag gccattaact
      601 ttaagattga agatgaaaag atggccgtta ttgttcagga ggtggtggga aacaaattcg
      661 aaaactacta ctatccccat atcagcgggg ttgcacaatc atacaactac tacccctttg
      721 ggcacattaa ccccgaagat ggatttgcca atatagccgt ggggcttggc aagcatgtgg
      781 ttgaaggagg aaaatcctat cggttttgcc cccggtaccc aaccctaatt aactacaccc
      841 tggaggacct ggtaaaaaat tctcaaaccg attttctcgc cattgacctc agcaggccag
      901 acttcgactt gctttcaggt gacgaggctg cccttgcgcg tctggatatg agctatgccg
      961 agcaacatgg taatttaaag cattgtgcat cggtttatag ctccaacaat aatagcttga
     1021 ttgccggaat cgggcaaccg ggtccgaggg ttatcaattt ccctaatatt ctcaagtaca
     1081 actatattcc tctttcccaa acaattgatg taatcctcga cattgtaaag gaagcctttg
     1141 gctccccctg cgaaatagag tttgct
//
LOCUS       DAOOQX010000704         1165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_170102_length_1165_cov_2.253153, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000704 DAOOQX010000000
VERSION     DAOOQX010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170102_length_1165_cov_2.253153"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PLX53_09670"
     CDS             complement(<1..745)
                     /locus_tag="PLX53_09670"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOY73391.1"
                     /translation="MKLELICEQVVTEAKKAGSFAMEQRANFNINKVEVKGKHNFVSY
                     VDRTCEAMLVDGLSKIVPQAGFIAEEGTGKPNKGGLNWVVDPLDGTTNYIHGISPFAV
                     SIALMDDQKLLLGVVYEANLKECFWATADSQAYLNGAPIKVSETGKVNDALVATGFPY
                     YDYSRINPFFQTMEYFMRNSHGLRRLGSAATDLVYVACGRFDAFYEYGLSPWDVAAGA
                     LIVERAGGRVCDFKRGNDFIFGGEIMATNS"
BASE COUNT          341 a          309 c          219 g          296 t
ORIGIN      
        1 tgctgttggt agccattatt tcacctccaa aaataaaatc attaccccgc ttaaagtcgc
       61 ataccctacc tccggcacgc tccacaatca aagctccggc agccacatcc caagggctta
      121 gcccatactc gtagaaggca tcaaatcgtc cgcaggccac gtaaaccaaa tcggtagcag
      181 cagagcctaa ccgtctcaaa ccatgggaat tacgcatgaa gtattccata gtctgaaaaa
      241 acgggttgat gcgagagtag tcgtagtagg gaaaaccggt tgcaacaagg gcgtcgttca
      301 ccttaccggt ttccgacact ttaataggtg caccatttaa ataggcctgg ctgtcggcag
      361 ttgcccagaa gcattccttt aagttggcct catacacaac gcccagcaac agtttttgat
      421 cgtccatcag ggcaatactg accgcaaaag gggatattcc atgaatgtag ttggttgtcc
      481 catccagggg atccaccacc cagttcagcc cccctttatt tggcttaccg gttccctcct
      541 cagcaataaa gccggcttga gggacaattt tcgagagacc atcaaccagc atcgcctcgc
      601 atgtccggtc aacatacgat acaaaattat gcttgccttt cacctcaacc ttattgatgt
      661 tgaagtttgc ccgttgctcc atggcaaagc tacccgcctt tttggcttcg gtaacaacct
      721 gttcacaaat caactctaac ttcacgttaa tattcagctt aataagtgtt tatgccaaca
      781 aaaaaataac tttcaacctg catacatatc atatagcatg caagcgcata gctaagatga
      841 ttaatgcaaa ttagtccgta ttttagctat gtaattcacc agcatcaatt tgatcagcat
      901 gcagctttcc aaaactttaa aggtattcag gaggaatttc tgaaaaaatt ttctccagtt
      961 attaaagcag caagcccatg cctgataaac tctaaaagcc caactccgac tcaattggag
     1021 caagtgcctc cagcgaaagg ctgacgaaat cgtcaatcga aagccctaac aattcacact
     1081 ccatgatagt gctacgattc acagaggcgg caaactgctt ctccttcatt cttttgacaa
     1141 ccgacttagg cttaacgctg ctaat
//
LOCUS       DAOOQX010000705         1164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_170243_length_1164_cov_3.248873, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000705 DAOOQX010000000
VERSION     DAOOQX010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170243_length_1164_cov_3.248873"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..953
                     /locus_tag="PLX53_09675"
     CDS             105..953
                     /locus_tag="PLX53_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOY73392.1"
                     /translation="MVDAIIEFFSYGFVRNAIIASALISILVGFIGTYVVSRRLVFLS
                     GGITHASFGGIGIAWYLGWNPLLGAAIFSIVSAVGIEYLTGEKRLREDSAIGMLWALG
                     MALGVFFVFLTPGYAPDLMSYLFGNILTISTSDNLMLLVLSLVTALFFTLFYYPILFL
                     SFDEEYVKTQHVKARVINFTLKILVALAIVLSIRAIGIILVISLLTIPANIANLFTKR
                     FGNIIWLSALICFGSTILGIILSYRLNVPSGATIVIALALIYLLTRFYFHLKGAIFRD
                     SLRKGL"
BASE COUNT          292 a          281 c          221 g          370 t
ORIGIN      
        1 aacaccatgc cgataacaac ttaacagaat agtaaacata aactaaaggc cacaattaaa
       61 ctaccgccgc ctttgcggat aatcaacaat taggtaaaaa caaaatggtt gacgcaatca
      121 tagaattctt tagctacgga tttgtgcgga atgcaattat cgcctctgcg cttatctcca
      181 tactggttgg ttttattgga acatacgttg tatcgcgcag gcttgtattc cttagcgggg
      241 gaattactca tgcctccttc ggaggaatag gaattgcctg gtacctggga tggaatccgc
      301 tgcttggcgc tgccattttc tccattgttt cggccgtggg tattgagtac cttaccggcg
      361 aaaagcgtct ccgcgaggat tcggctattg ggatgctctg ggcgttgggc atggcgcttg
      421 gtgtgttttt cgtttttcta acccccggct atgcccccga cctgatgtcg taccttttcg
      481 gcaatattct caccatttct accagcgaca acctgatgct gctggtgcta tcacttgtta
      541 ccgccttgtt ttttaccctt ttctactacc ccattctttt tctttccttt gacgaggagt
      601 atgttaaaac ccagcatgta aaagccaggg tcatcaactt tacgcttaaa attcttgtgg
      661 cccttgccat tgttctctcc atcagggcta ttggaattat ccttgtgatt tcgctattaa
      721 ccatacctgc caacattgct aacctgttta ccaagcgttt tgggaacatc atctggctat
      781 cggcgttaat ctgcttcggc tcaactattc tgggaataat tctttcatac aggctgaacg
      841 ttccctcagg agcaacaata gtaatcgccc tggcactaat ctacctttta acacgattct
      901 actttcacct taaaggagct atctttaggg attcgctaag gaaaggcttg taaacacctt
      961 actacgacat aattcacacc tacaactaat cggataacca gattaacctg gccagttttc
     1021 tatgccattc ttctaaaaaa actacacgcc ctcatttgct tatcaagctc tttgatgatt
     1081 ggctattgca cagctttgaa tgtattcatt aacccaaaac cctaaatttt taggtaatag
     1141 atattttcat tttatatagt agct
//
LOCUS       DAOOQX010000706         1163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_170510_length_1163_cov_2.888989, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000706 DAOOQX010000000
VERSION     DAOOQX010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170510_length_1163_cov_2.888989"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLX53_09680"
     CDS             complement(<1..320)
                     /locus_tag="PLX53_09680"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOY73393.1"
                     /translation="MKNRFLAIFRKSEILQNASILVSGTILAQAIPILLQPFLRRYFS
                     PHDFGVYSVYSSLIGILLVISSFRYEQAIVLPKRNRDASVLVTSSILFSLVLLLSSSQ
                     PFTS"
     gene            complement(447..>1163)
                     /locus_tag="PLX53_09685"
     CDS             complement(447..>1163)
                     /locus_tag="PLX53_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73394.1"
                     /translation="QVKPIIRLIVHFGVATASLYLLGGLRKPVTPGIDILSIPAVINP
                     LAVVGMVWFINLYNFMDGADGFASVEAISIALVLFAFTGSMELLLLVSAVLGFLYWNW
                     PKAKIFMGDVGSTQLGFILVVLGINYHNTLDFSIFNWLMISSPFWFDATLTLFRRWRN
                     KEKLSEAHRKHTYQRFIQAGYSHKELDLALIFFNGTIFLMILVYREFEIMKIPITLFT
                     LGILYYFTRRVDRLFPFAKD"
BASE COUNT          392 a          225 c          242 g          304 t
ORIGIN      
        1 cacgaggtaa aaggttggct tgatgataaa agtaaaacta aactaaaaag aattgatgat
       61 gtaacgagta ctgaggcatc cctatttctt ttaggtagca caatagcctg ttcgtaacgg
      121 aaactggaaa ttacaagcaa aattccaatt aaactgctat aaaccgagta aaccccaaaa
      181 tcgtggggtg agaagtagcg acgaagaaat ggttgcagca gtatgggtat ggcctgagcc
      241 aaaatggtac cgcttaccag tattgaagca ttctgaagga tttcagattt tctaaaaata
      301 gctaaaaagc ggtttttcac cgaaattcgt ttttaatcta agtgttcatc aaattaacaa
      361 tcttaacggc ttgatgtaaa cccgacatgt ggtatgtgct ttttttactt ttgaatttgt
      421 gtttctcaga aatggggatt tcggtattaa tcctttgcaa aagggaaaag acggtcaacc
      481 ctgcgggtaa agtagtataa aatgccaagg gtgaataggg taattggaat tttcataatt
      541 tcaaattccc tatatactag aatcataaga aagattgtgc cattaaaaaa tataagggct
      601 aaatccagct ccttatgtga atagcctgcc tgaataaatc gttggtaagt gtgtttacga
      661 tgagcctcgc tcaacttttc cttatttcgc caacgtcgaa aaagtgtaag ggttgcatca
      721 aaccagaatg gtgacgaaat catcaaccag ttaaaaattg agaaatcaag cgtgttgtga
      781 tagtttatcc ccagcacaac cagaataaaa cccagctggg tacttcccac atcgcccata
      841 aaaattttgg cttttggcca attccaatac aaaaatccca gcacagccga aactagaagt
      901 aaaagttcca tgcttccagt gaaagcgaaa agaactaggg cgatagaaat tgcctcaacc
      961 gaggcaaacc cgtcagcgcc atccatgaag ttgtacaggt tgataaacca gaccattccc
     1021 acaacggcca gcggattgat tactgcggga atgctaagta tatcaatacc gggggttacc
     1081 ggtttgcgta aacctcccag caggtataaa gaggcagtag ccacaccaaa atgaactatc
     1141 agtctaatta tgggctttac ctg
//
LOCUS       DAOOQX010000707         1162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_170731_length_1162_cov_3.364047, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000707 DAOOQX010000000
VERSION     DAOOQX010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170731_length_1162_cov_3.364047"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PLX53_09690"
     CDS             <1..793
                     /locus_tag="PLX53_09690"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOY73395.1"
                     /translation="FYQTFAPNLKEWPNRSAEGDPYKGITPYYQIVEPISLYPFSTKP
                     EKKISVQDVMAFQRSTFEGTIYDMTNDPSWLVPNGKGGYEKSPLATPFPGSDLRKLLK
                     ITYRRPVARHRGHYGMVLQLRGWLPNEIGGVYWVYLDNPYFSPYVPIYAGNLSVHDSY
                     STYDPNRYDEKSARWAIDFVDNLANLKFQAIAADVRAVRSPFEAKIFENQAAVETEAL
                     RLYKKNPQSAQEYLTGYTNGLMSDVTAMFLGLRNQIITNYTNNHE"
     gene            complement(886..>1162)
                     /locus_tag="PLX53_09695"
     CDS             complement(886..>1162)
                     /locus_tag="PLX53_09695"
                     /inference="COORDINATES: protein motif:HMM:NF021546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOY73396.1"
                     /translation="SGSKQSVQIFVFSKKYQEIADRITGDLHRGVTVVDGVGWFSKEP
                     QKVLITLVRKYESNDVYKIIKEIDPEAFLSVSTVMGVYGRGFDVIKH"
BASE COUNT          318 a          300 c          242 g          302 t
ORIGIN      
        1 tttctaccaa acatttgccc caaaccttaa ggaatggccc aaccgtagtg ccgagggtga
       61 tccctacaag ggaataaccc cttactacca gattgttgaa ccgatttcgc tttacccgtt
      121 ctcgactaaa ccggagaaaa aaatttccgt tcaggatgtt atggcatttc agcggtcaac
      181 cttcgaggga accatttacg acatgactaa cgatcctagc tggcttgttc ccaatggtaa
      241 aggtggatat gagaaaagtc cgctggcaac gccattcccg ggtagcgact tacgaaagct
      301 actaaagatt acctatcgtc gtccggttgc ccgccatcgt gggcattatg gaatggtgct
      361 ccagctacgt ggctggcttc caaatgagat tggtggggtt tactgggttt acctcgacaa
      421 tccctacttt agcccgtatg taccaatata tgcaggaaac ctatcggttc atgattccta
      481 ctcaacctat gatccaaacc ggtacgacga gaaatcggcc cgatgggcaa ttgattttgt
      541 tgacaacctg gccaacctca aatttcaggc tattgcagct gacgtcaggg cggtcaggtc
      601 gccttttgaa gccaaaattt ttgaaaatca agctgctgtt gagacggaag cacttagact
      661 gtacaaaaaa aatccacaat cagcacagga gtaccttaca ggttacacca acgggctgat
      721 gagcgatgtt acggcaatgt ttcttggttt acgcaaccag ataatcacga actacaccaa
      781 caaccatgaa taggtaacac taccagcatg aagaattgca gaaaggccgg tagcttaccg
      841 gcctttctgc actctatact tccgtttact ctttaacccc ggttcttaat gcttaattac
      901 atcaaacccc ctgccatata ctcccatcac ggtagaaacc gaaaggaatg cttcggggtc
      961 gatttccttg attatcttgt aaacatcatt tgattcatat tttcgcacca gggtaataag
     1021 caccttttgg ggctccttgc taaaccagcc taccccgtca accaccgtta ctcccctgtg
     1081 caagtccccg gtaatcctat cggcaatctc ctgatatttt ttggaaaaga caaaaatttg
     1141 cacggactgc ttgctgcccg aa
//
LOCUS       DAOOQX010000708         1160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_171276_length_1160_cov_2.450679, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000708 DAOOQX010000000
VERSION     DAOOQX010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171276_length_1160_cov_2.450679"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            480..>1160
                     /locus_tag="PLX53_09700"
     CDS             480..>1160
                     /locus_tag="PLX53_09700"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HOY73397.1"
                     /translation="MKRNIIQRGITMVLLTAGSISAFGQVEDPVQWSISSKKIDAKKY
                     ELIITATIEQGWHLYSTKLPEGGPLPTSFNFADSKDYKLVEGVKELTKPKTEHDDIFN
                     LDLSFFEGEAQFGQIVDLAGDEASINLTVEYQACYADKCIYLEKDLSHKLGKNGSVVL
                     AGEEVQPEKTTTATLAPTNDESAKKSSSSLWVFFFVSFAAGLAGLLTPCVFPMIPMTV
                     SFFMGKQGN"
BASE COUNT          372 a          242 c          254 g          292 t
ORIGIN      
        1 cagctgtaaa gtgcatttgc agcacgctcc atcaattcat atgaggatat tggctcatgc
       61 ttaatggtgt aggcatcgag ttcgcgaatc tgttttgttg taaacaactt catgctgaaa
      121 gctaccctgg ttgacttttt ttggctcaat ggggtactac aaaagtagaa aatgtggcga
      181 ttttttctta acaaaagctc ttaaatgaca aaaatcaaaa agtaagccga ttcaaaatct
      241 atatatttgc cgcaacaaaa actacgaggg aaaatttaat tttgctggtg caccagcgaa
      301 taaaatgatt acaactttct atggcccgaa gttgttaacc cgtaacccac aaacgaatta
      361 tggatttttg tagaaaagcc ggtaagtgat gagcaagata gagataaaat tcactaacct
      421 cgacaaggta gcttaagtat tttttctcac aattcaacat gtaaccaaaa acgtggcaaa
      481 tgaaacgaaa cattatccag aggggaataa ccatggtgtt gctcaccgcg ggcagcatta
      541 gtgcatttgg acaggtagaa gacccggttc agtggagcat ctcgtcaaaa aaaattgatg
      601 ccaaaaaata cgaacttatt ataacagcca cgatagagca aggctggcat ttgtactcaa
      661 caaagctgcc cgagggcgga cctctgccca ccagctttaa ctttgccgac agcaaggact
      721 acaagctggt tgaaggggta aaagagctaa ccaaacccaa aacggagcat gacgacattt
      781 tcaaccttga cctttcgttc ttcgaggggg aggcgcagtt tggtcaaata gttgaccttg
      841 caggtgatga ggcaagcatt aaccttacgg ttgaatatca ggcttgctac gccgacaagt
      901 gcatatacct tgaaaaggat ttaagccaca agctaggaaa gaatggttct gtagtacttg
      961 ccggtgaaga ggttcaaccc gaaaagacaa caactgcaac ccttgcacca acaaatgatg
     1021 aaagtgcaaa aaaatcaagc agctccctat gggtattttt ctttgtatcg tttgctgcag
     1081 gcctagccgg attgctaaca ccctgcgtgt tcccgatgat acccatgaca gtttccttct
     1141 ttatgggaaa gcagggcaac
//
LOCUS       DAOOQX010000709         1159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_171480_length_1159_cov_2.778986, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000709 DAOOQX010000000
VERSION     DAOOQX010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171480_length_1159_cov_2.778986"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PLX53_09705"
     CDS             <1..710
                     /locus_tag="PLX53_09705"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOY73398.1"
                     /translation="WLFRYRGVLPLIILAVGAFIYIRAELNPSPSSFMEVNSYKSIYE
                     ISCLLVSLFGLAIRVYTIGHTPSKTSGRNIKEQVAESLNTTGIYSVVRHPLYLGNFFM
                     WLGPAMLTANFWFIVSFCLAYWIYYERIMFAEEQFLRRKFGEAYTEWASRVPAFIPCF
                     KHFKKPCHSFCTRKVVAKGKNGLLAVFLIFCSFNVVGELVHKGKHHFNMIFIGMCILS
                     IVLYIVVKIFRKKINLV"
     gene            complement(761..>1159)
                     /locus_tag="PLX53_09710"
     CDS             complement(761..>1159)
                     /locus_tag="PLX53_09710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOY73399.1"
                     /translation="PHTVIDTIEVNDELEETIQTYTSMAGVDSKIRLHLGDACQIIPT
                     LDYTYDFAYIDGDKREYPEYYKLVLPKVNSGGLIIADNVLWGGKVVDASENDEHTKAL
                     RAFNTMVQNDMGVENVLLPLRDGIMLIYKL"
BASE COUNT          286 a          253 c          265 g          355 t
ORIGIN      
        1 actggctatt caggtacagg ggcgtgctac cacttataat tttggctgta ggggctttca
       61 tctacatacg agctgaactt aaccctagcc catcatcatt tatggaggtt aatagctata
      121 agtcgattta cgaaatatcc tgcctgctcg tcagcctctt tggactggcc ataagggttt
      181 acacaattgg gcatactcca agcaaaactt caggtcgaaa cataaaggaa caggtagccg
      241 aatcgctcaa cactactggc atatactcag tcgtcagaca cccgctctac ctcgggaatt
      301 ttttcatgtg gctaggtcct gccatgctga ctgcaaactt ttggtttatc gtttcattct
      361 gcctggcata ctggatttac tacgaacgga ttatgtttgc cgaggagcaa tttcttaggc
      421 gaaaatttgg cgaagcctat acggagtggg ctagtagagt tcctgccttt attccctgtt
      481 ttaagcattt taagaagccg tgccattcat tctgcactag gaaggtggtg gctaagggaa
      541 aaaatgggtt gctggctgtt ttcctaattt tctgctcgtt taatgtagtt ggcgagcttg
      601 tgcataaggg taagcaccac ttcaacatga tttttattgg gatgtgcatc ttgtctatag
      661 tgctttacat agttgttaaa attttcagga agaagataaa tcttgtatag cagtagtaaa
      721 gacaatttgc taaagctggt tttccctgct tacattccag ttataacttg taaatcaaca
      781 taataccgtc gcgtaagggg agcaggacgt tttctacccc catatcattt tgaaccatgg
      841 tgttgaacgc cctcagggct ttggtgtgct cgtcgttttc actagcatca accactttcc
      901 ctccccagag cacgttatct gcaataatta accctccgct gttaaccttg ggtagaacaa
      961 gcttgtagta ctcggggtac tctcttttat cgccgtcaat gtaggcaaaa tcgtaggtgt
     1021 agtcaagggt agggataatc tggcaggcat cgccaaggtg cagcctgatt ttgctatcaa
     1081 ccccggccat ggaggtgtag gtttggatgg tctcctccaa ctcgtcgttt acctcaattg
     1141 tatcaataac tgtgtgggg
//
LOCUS       DAOOQX010000710         1155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_172452_length_1155_cov_3.280909, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000710 DAOOQX010000000
VERSION     DAOOQX010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172452_length_1155_cov_3.280909"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1155)
                     /locus_tag="PLX53_09715"
     CDS             complement(<1..>1155)
                     /locus_tag="PLX53_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOY73400.1"
                     /translation="ILTVVIAIASIGLIFLQSRWIGVALQIKEEQFVQTASLAMERII
                     DEVEKQETVFQVIDEIKPYYSVNTKSSARLDYQQNILNKTKSGIRTKQINQQVLTFSS
                     LDSVKIPSITKKLWDSSLVSINNGDLHIPSLSKGKNLKNIDLGLDEKLVNKTIFVENI
                     VDKMIRVELPIQERISQVQLDSIVRRELLRKGIKARYEYRITNERDSTIYASPKFKAD
                     FKGLVLKDKLFPNDFFARRYFLTLYFPNQKTYLLNSLGRITLSSLALTFLIVFTFSVT
                     LYIIFKQKKISEIRNDFVNNLTHELKTPISTISLAAQMLNDKSIPLERKNLDYLGTLV
                     ADESKRLGLQVEKVLQMAIFEKAKLKLRLKEVNVHEIIKRVSNNFALQLQS"
BASE COUNT          267 a          251 c          284 g          353 t
ORIGIN      
        1 ggattgaagc tggagggcaa agttgttgga tacccgcttg ataatttcgt ggacgttcac
       61 ctccttgagc cgaagtttta gcttggcttt ctcaaagatg gccatttgta gcaccttttc
      121 cacctgcaac ccgagccgtt tgctctcgtc ggcaaccagg gtgccaaggt agtctaaatt
      181 ttttcgttca agcggtatag atttgtcgtt gagcatttgc gctgccagcg aaatggtgga
      241 gatgggcgtt tttagctcgt gcgtaaggtt gttgacgaaa tcgtttctta tctccgaaat
      301 ttttttctgc ttgaatatga tgtaaagggt tacgctaaag gtgaagacaa ttagaaaggt
      361 gagcgccagc gatgaaaggg tgattcgacc cagcgagttg agcaggtatg tcttctggtt
      421 ggggaagtaa agggtaagga aatacctgcg tgcaaagaaa tcgtttggga aaagcttatc
      481 ctttaacaca agacctttga aatcggcctt aaacttaggg ctggcataaa tggtgctatc
      541 cctttcgttg gttatcctgt actcgtagcg ggcttttatt cccttgcgta gcaattcacg
      601 gcgcacaata gagtcgagct gtacctgcga aatgcgctcc tgaatgggca gctccacccg
      661 aatcatcttg tcaacaatgt tctccacaaa aatggtcttg ttaaccagct tctcatcgag
      721 tcccaggtca atatttttca aatttttacc cttcgatagg ctgggtatat gcaggtcgcc
      781 gttgttgatg ctcaccaagc tgctgtccca taacttcttg gtgatggagg gtattttcac
      841 agaatcaagg ctgctgaagg tgagcacctg ctggttgatt tgcttggtcc gaatcccgct
      901 ttttgttttg ttcagaatgt tttgctggta gtctagcctg gcgctgcttt tggtgttgac
      961 cgagtagtat ggctttatct cgtcaattac ctgaaaaact gtttcctgct tttcaacctc
     1021 atcaataatt cgctccatgg caaggcttgc cgtttgaaca aattgctcct cctttatctg
     1081 cagggcgacc cctatccacc tcgactgtag gaatatcagg ccgatagatg cgatggcaat
     1141 aactactgtt agaat
//
LOCUS       DAOOQX010000711         1155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_172556_length_1155_cov_2.220909, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000711 DAOOQX010000000
VERSION     DAOOQX010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172556_length_1155_cov_2.220909"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PLX53_09720"
     CDS             <1..736
                     /locus_tag="PLX53_09720"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOY73401.1"
                     /translation="GFQAEVVDAKDAIFTFKDQKVKFLWMSMPPDPRFIVSYRLIPIN
                     GNGEAVVSLSGKFSYLVGEATRVVDIIQKDVDLGNLDPKSIEGLLASIPGAEIAAPSS
                     SSFSSSFTSAGSDGGIDIPVQYQVIPEKSKKIAQPEQNMMLYMLEPEKGVYYRVQVAA
                     GHKPINIKRYFLRHNVTYEVRTEKHEGWYKYSIGSFKEYKEARDFRVQIWNTTKINDA
                     FVAAYNNGVRITVQEALMITNQTWFR"
     gene            755..>1155
                     /locus_tag="PLX53_09725"
     CDS             755..>1155
                     /locus_tag="PLX53_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73402.1"
                     /translation="MRRFIARNKITLFTLCISGMLLCTYNSAHAQDDEEYYKNLLDTV
                     VEVINPVYKPVLSFGAGIMSFWGDVQNPVTNPVNGKYGFKFNVSTLFGKNYFKGNIFA
                     IYGQFQGQNFDITRQMQLQPLPLDDIGNPIYP"
BASE COUNT          330 a          247 c          261 g          317 t
ORIGIN      
        1 gggctttcaa gcagaggtgg tagatgccaa ggatgccatt tttactttta aagatcaaaa
       61 ggttaaattt ctatggatga gtatgccgcc cgaccctcgc ttcattgtgt cgtaccggct
      121 aattcctatt aacggaaacg gggaagccgt tgtcagcctt tcgggaaaat tttcctacct
      181 tgttggtgag gctacacggg tagttgacat tatccaaaag gatgttgact tgggcaatct
      241 cgacccgaaa tccatcgagg gattgctggc ctccatcccg ggcgctgaaa ttgcagctcc
      301 ctcttcttca agcttcagca gcagctttac atctgctggc tccgatggtg gaatagatat
      361 tcctgttcag taccaggtaa ttccagagaa atcaaaaaaa atagcccagc ccgaacaaaa
      421 catgatgctc tacatgctgg agcctgaaaa aggggtttac tacagggttc aggtggctgc
      481 cgggcataag cccatcaaca tcaaaaggta ttttttacga cataatgtta cctacgaggt
      541 gcgaaccgag aagcatgagg gttggtataa atactccata ggatcgttta aggagtacaa
      601 ggaagcgcgc gactttaggg tgcaaatatg gaataccact aaaattaacg atgcgtttgt
      661 ggccgcctac aacaacggtg ttagaattac cgttcaggaa gcactaatga taaccaacca
      721 aacttggttc agatagctag ctgttaaagt tctgatgaga aggtttattg ccagaaataa
      781 aattacactc tttaccctct gcatttcggg tatgctcctt tgcacctata attcagccca
      841 tgcacaggat gatgaggaat actacaaaaa cttgctcgat acggttgttg aggttattaa
      901 ccccgtttac aaaccggttc tatcatttgg tgcaggaatc atgagttttt ggggggacgt
      961 tcaaaaccct gtaaccaatc ctgttaacgg aaagtatggt ttcaaattta acgtttcaac
     1021 gctttttggg aagaactact ttaagggaaa tatttttgcg atttacggcc agtttcaggg
     1081 tcagaacttt gatattaccc gtcagatgca gctacaacca ttacccctag acgatattgg
     1141 aaatcccatt tatcc
//
LOCUS       DAOOQX010000712         1155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_172574_length_1155_cov_2.136364, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000712 DAOOQX010000000
VERSION     DAOOQX010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172574_length_1155_cov_2.136364"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1155)
                     /locus_tag="PLX53_09730"
     CDS             complement(<1..>1155)
                     /locus_tag="PLX53_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73403.1"
                     /translation="SGTTLNSADQEALGMLSNQISVQVESRYDLLRQEVTGSGKQTFE
                     EVFKGVISTYSSATLRNTERIVISNEPDAKVFRYIKRDEIDKIFSDRERKIVEFARNG
                     EQAALSGRVADALRYYYWSLTLLRSHPNANTIGMKDEKGNDRLLISWLPMQINTVLAD
                     VRVTIAGTNRMDDLTRYFLNIRYQNEPARNFDYSYWTGRDWTNIYSAKDGIGIAEFFG
                     KSDINEIRIKAEYVFEGETAIDNELRDVMAKLSVVPFRGSYFSVPVGKVEEPISLPET
                     AGGVTMVQDTKPYEEKMNSLLNSIRTQNYQAAQSLFTPEGYEMYKSLLQYGQARIVKE
                     PEFKFIHFEKVVMCRALTMSFHFKNNNRNFIEDVVFQFNEEKKISNISFG"
BASE COUNT          276 a          275 c          241 g          363 t
ORIGIN      
        1 aatccgaagg agatatttga tattttcttc tcctcattaa attgaaaaac aacatcctct
       61 ataaagttgc ggttgttgtt cttaaaatgg aagctcatgg tgagcgccct acacataacc
      121 actttttcaa aatggataaa cttaaattcg ggctctttta ctatccttgc ctgcccgtac
      181 tgcagaagac ttttatacat ctcgtaaccc tcaggggtaa aaagtgattg cgctgcctga
      241 tagttttggg tacggattga gttgagcagg gagttcatct tttcttcgta gggtttcgtg
      301 tcctgtacca ttgtaacacc tccagctgtt tctggcaggc taattggctc ttcaactttt
      361 ccaacgggca cgctaaagta gctgccacgg aagggaacca cgcttaactt tgccattaca
      421 tccctcagct cattatcaat ggcggtttcg ccctcgaaaa catattcggc cttaatcctt
      481 atctcgttta tatctgattt tccaaaaaac tctgcaatgc caataccatc ctttgcgctg
      541 taaatatttg tccagtccct gccggtccag tagctgtagt cgaaatttcg tgctggttcg
      601 ttttggtagc gaatatttaa aaagtaacgg gttaaatcat ccatcctgtt ggttccggca
      661 attgttaccc taacatcagc gagaacagtg tttatctgca tgggcagcca ggatattagt
      721 agcctgtcat tccctttctc atccttcatg ccaatagtat tggcgttggg gtggctacga
      781 agcagagtta gcgaccagta gtagtagcgc agggcatcgg caaccctgcc gctgagtgca
      841 gcttgttcac cgttacgtgc aaattcaaca atttttcttt ccctgtcgga gaatattttg
      901 tctatttcgt cccgcttgat atacctgaaa accttggcat caggttcgtt gcttattaca
      961 atacgctccg tattcctaag cgttgctgat gagtaggtgc ttatcacccc tttgaaaact
     1021 tcttcaaagg tttgtttacc cgaacccgtt acttcctgtc gcagtaggtc gtatcggctc
     1081 tcaacctgaa cagaaatctg attggaaagc attcccaaag cttcctggtc ggcgctgttg
     1141 agggttgttc cacta
//
LOCUS       DAOOQX010000713         1150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_173930_length_1150_cov_1.999087, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000713 DAOOQX010000000
VERSION     DAOOQX010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173930_length_1150_cov_1.999087"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLX53_09735"
     CDS             <1..560
                     /locus_tag="PLX53_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOY73404.1"
                     /translation="LVRYAQTIHAKRSSFVEQLIPIFMDYYNRVSGGEERVSIAYRSH
                     LNDGDFKTMLRNNIQRDRTLQFTSIGIHRDDMVLSIDGYPIRREGSQGQQKTFLIALK
                     LAQFEFLSRVSGIKPLLLLDDIFDKLDMDRVGFLINQVAHDGFGQIFITDTSKNRLDM
                     ILNRLNSGFRLFSIENSEVSIIAST"
     gene            565..855
                     /locus_tag="PLX53_09740"
     CDS             565..855
                     /locus_tag="PLX53_09740"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOY73405.1"
                     /translation="MSNHNTKSLGEAIREFITAMKWDEKINETSIIGDWEKIVGRIVQ
                     MHTTKIKLKDGQLTIQVDSSALRHELHMRKQQLIKRINDFYHASIVSDIVLR"
BASE COUNT          348 a          288 c          231 g          283 t
ORIGIN      
        1 agcttgtccg gtatgcccaa accattcatg ccaagcgcag cagctttgtt gagcagctca
       61 tccccatttt catggactac tacaacaggg tatccggcgg agaagaaagg gtgtccatag
      121 cctacaggtc gcacctcaac gatggcgact ttaaaaccat gcttcgcaac aacattcaaa
      181 gggatagaac tttgcagttt acctccatag gcatacaccg cgacgacatg gtgcttagca
      241 ttgacgggta ccccattcgc cgcgagggtt cacagggcca gcaaaaaacc ttcctcattg
      301 cccttaagct agcacaattc gaattcctgt cgagagtctc cggaattaaa cccctgctgc
      361 tgctcgatga catcttcgat aagctcgata tggacagggt tggctttctg attaatcagg
      421 ttgcccatga cggatttggc cagattttca tcaccgatac cagcaagaac cggctggata
      481 tgatactaaa caggctcaac tccggattcc ggctttttag tatcgaaaac tcagaagtat
      541 ccataatcgc ctcaacataa aaccatgagc aaccataaca ccaagtcgtt gggcgaagcc
      601 atcagggagt ttattaccgc catgaagtgg gacgagaaaa taaacgaaac ctccatcatt
      661 ggcgactggg aaaaaattgt tgggcgaatt gtgcagatgc acactaccaa aatcaagcta
      721 aaagacggac agctcaccat ccaggttgac tcctcggcgc tccgccatga actccacatg
      781 cgtaaacagc agctcatcaa aagaattaac gacttttacc atgcctcaat tgtaagtgat
      841 atcgtgctca ggtagccctt gctataaaca tttgttgtaa ccacttacca gaatgaagct
      901 ttggatagac aataccatta caacaagcca gccaacatca tcgttacaaa agccgttcta
      961 aataaacctc agaacttttg cagctgcttc ctttaccggt aaaaataaat gccacttaat
     1021 tcggtaatgc tctgctaaag aatgttataa acgcaatcct ttgtgtacat ttttgcaatg
     1081 taccagctac ctttatagag caaaaaaatt catttttccg gttgctaaca attaatttgc
     1141 tggagacagg
//
LOCUS       DAOOQX010000714         1148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_174330_length_1148_cov_2.935956, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000714 DAOOQX010000000
VERSION     DAOOQX010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174330_length_1148_cov_2.935956"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1148)
                     /locus_tag="PLX53_09745"
     CDS             complement(<1..>1148)
                     /locus_tag="PLX53_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73406.1"
                     /translation="LLVVSFGCHKPKPYDLIVENAIIFETTEGKASPALKTILINADT
                     IAGIVDYETPVRTKKVVDADGRLVTPGNIDTHVCLQNVIEGSKNPQSSYPKVLTNYYR
                     NNFSRRYLPYGITTALDLEPNDSWTSQILDWTPNPKFTTLLVAYQLPKVSFISSNRPT
                     TPNIDSIAKALQSKGLGYVFLDEQEAVFPQKVYEMLKGKGMVVFTRPHFGTFLRDSSK
                     NIVSISTLISNALHYISKQNDLSRQIKNVYGSIDTFPKELFILEAFKYMVENEPALLD
                     KIAQEFGNRKATISSDLYGMAEFAGLTPERNLSLTLPRELQHRAEYNFNHLLSYVKKL
                     HDMGVKLLIGTNSPLGGLAYETEQMLLSEAGIPASDVLVISTLNAEAG"
BASE COUNT          287 a          231 c          271 g          359 t
ORIGIN      
        1 ccctgcctca gcgttaagag ttgatattac tagtacatct gaggcaggga ttccagcctc
       61 agacagcagc atctgttctg tttcataggc cagccctcct aacgggctat tagttccgat
      121 gaggagtttt acgcccatat cgtgaagttt ttttacatag ctgagcagat ggttgaagtt
      181 gtactccgct ctgtgctgca gctcgcgtgg aagggttaag gataaattcc tttcgggtgt
      241 gagtccggca aattcggcca ttccatacaa atcagatgat atggtagcct tcctgttacc
      301 aaactcttgg gcaatcttat caagcagcgc gggctcattc tctaccatat acttgaatgc
      361 ttccagaatg aacaactcct ttgggaaggt atcgatgctg ccatagacat ttttgatttg
      421 acgcgaaaga tcgttttgtt tagaaatgta gtgcagtgcg ttgcttatta gtgttgaaat
      481 gctgactata tttttgctgg agtcccttaa aaaagttcca aaatggggac gagtaaaaac
      541 aaccattcct tttcctttca gcatttcgta aactttttgt ggaaaaacag cttcctgttc
      601 atccagaaaa acatatccta gcccctttga ttgcagagcc ttagcaatgc tgtctatatt
      661 gggagtggta ggtctattgg agcttataaa tgaaactttt ggaagctggt aggctacaag
      721 cagggttgta aatttaggat tgggtgtcca atccagtatt tggctggtcc agctgtcatt
      781 gggctctaag tctaaagctg tggtaattcc gtagggcaaa tacctcctgg agaagttgtt
      841 gcggtagtag ttggttaaaa ctttcggata gctgctttgc gggtttttgc taccctcaat
      901 cacattttga aggcaaacat gggtatcaat attaccgggt gttacaagcc gaccgtcggc
      961 atccactacc tttttggttc ttacaggtgt ttcgtaatca actattccgg caattgtgtc
     1021 ggcatttatc aggatggttt taagtgctgg agatgctttg ccctcggtag tttcaaaaat
     1081 tatggcattt tcaacaatca ggtcgtaggg ttttggctta tggcagccaa aggaaactac
     1141 cagcaatg
//
LOCUS       DAOOQX010000715         1147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_174669_length_1147_cov_2.289377, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000715 DAOOQX010000000
VERSION     DAOOQX010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174669_length_1147_cov_2.289377"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..>1147)
                     /locus_tag="PLX53_09750"
     CDS             complement(208..>1147)
                     /locus_tag="PLX53_09750"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOY73407.1"
                     /translation="RYSKPSERLRETCMLCMRCLKACPVGIEITQIRKNERSEHQLFS
                     PTSYSYLNGYAYPKAKVAFFAGCMGHLTPNIIKSTLLLLDAAGIDYTFIDRNGSICCG
                     RPLQLNGEVDAAKQLIDKNNRLIYETGANCLVTTCPICASEFKKNYHMSIPIYHHSQY
                     LWELTNQGRLVFNQSNLNVAYHDPCELSRGLGVVDEPRKLLKSAVVLTENSNQTKQNS
                     LCCGGSLAITNTSAFQKSTIAALTVLQLANNTTDLIATACPLCKKTLAAAQNDVPVQD
                     ISEIMVKALMDSRKASLSEAKVKMASPKLEVVEEYK"
BASE COUNT          284 a          234 c          280 g          349 t
ORIGIN      
        1 aattagaact taggataaaa aatgaattcc ttctggattt attacactta tggtctagtc
       61 ctttaacgaa gaaggatgtt gaaattgatt acatgtctta aaaaaaattt gattaaggtt
      121 gtgtaatcct aatcattagt tgctggaaag taataaatac cggcttgaaa gttttgaaag
      181 gcagaatgcc atgagtactt accgcaccta cttgtattcc tcgactacct caagtttggg
      241 gctagccatc ttaactttag cctctgaaag gcttgcctta cggctgtcca taagcgcttt
      301 caccataatt tcggatatat cctggacggg gacatcattt tgggcggcag caagggtttt
      361 cttgcaaagc gggcatgctg tggctatcag gtcggtagtg ttattggcta gttgcaaaac
      421 ggttaatgcg gcaattgttg acttttgaaa tgcagaggtg ttggtaattg caaggcttcc
      481 cccgcagcat agggaatttt gtttggtttg gttgctgttt tcggttagaa ccacagcgct
      541 ctttaggagc ttgcgtggct cgtcaacaac accaagacca cggcttagtt cacacggatc
      601 gtggtaagca acattcaggt tggattggtt aaaaaccagc ctgccttggt tggtaagctc
      661 ccagaggtac tggctatggt ggtatatggg aatgctcatg tggtagttct tcttaaactc
      721 gctggcgcaa atggggcagg tggttaccaa gcagttggcg cctgtttcgt aaataagccg
      781 gttattttta tcaataagct gctttgccgc atcaacctct ccgttaagct gcaacggcct
      841 accacagcaa atgcttccgt ttcggtcgat aaaggtgtaa tcgatcccgg cagcgtcgag
      901 aaggagaagc gttgatttaa ttatgttggg ggttaaatgc cccatgcacc ctgcaaaaaa
      961 tgcaacctta gctttggggt aggcgtaccc gttcaggtag ctatagctgg ttgggctaaa
     1021 aagctggtgc tcgcttcgtt cgttctttct tatttgtgta atttcaattc ctaccgggca
     1081 tgctttaaga catctcatac aaagcataca ggtttctcta agcctctcgc ttggcttgct
     1141 gtatcta
//
LOCUS       DAOOQX010000716         1143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_175613_length_1143_cov_3.647978, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000716 DAOOQX010000000
VERSION     DAOOQX010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175613_length_1143_cov_3.647978"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..>1143
                     /locus_tag="PLX53_09755"
     CDS             297..>1143
                     /locus_tag="PLX53_09755"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOY73408.1"
                     /translation="MDLESLYRKALALEPLSMEEGSYLYRHAPTHDLIYLGNELRQLH
                     VPSKKVTWQIDRNVNITNVCISGCKFCNFHTGLHSNNAYITTFPEYRQKVEELERYGG
                     DQLLLQGGLHPKLGLEFYVNLFTQLKSAFPNIRLHALGPPEIAHIAKIDGLSNSEVLQ
                     KLVESGLDSLPGAGAEILVDRVRKRISPGKPDSQSWLDVMAEAHKMGLVTSATMMIGH
                     VETIEERVEHLIKLRDLQNRKPTNAPGFVNFIVWTFQGKGTMLEKEGITPHVTDEEYI
                     RTIAIS"
BASE COUNT          347 a          284 c          242 g          270 t
ORIGIN      
        1 aaaacccaaa cgcgcattac cagcttgcta gaaaccaact aaaaaatttt aataaaacac
       61 cgatagaatt gatgagacag attctcacta attttttatc cgcatttatc agctattaaa
      121 gggtagctag cgtttagctt ttgagatttt ataaatcgct ggagaaaagt aaaaaaatcc
      181 cagcatagaa atttaccaaa gttgtcgcta accaatctac ctataaagta ggccatagct
      241 caacctcaac ccataaaaat taaatagcta attttacaaa attttacgag acacaaatgg
      301 atctggaatc tctttaccgt aaggctcttg cgcttgaacc cctatcgatg gaagagggtt
      361 catacctata ccgtcatgcc ccaacccacg acctgatata ccttggcaac gagcttcgcc
      421 agcttcacgt cccatcgaaa aaggtaacct ggcaaattga ccggaacgtt aacattacca
      481 acgtctgcat ttcgggttgt aagttttgca acttccacac cggtttgcat tccaacaatg
      541 cctacattac cacttttccc gagtacaggc agaaagtgga ggaactggag cgctacggag
      601 gcgatcagct cctgcttcag ggcggcctac atcctaagtt gggtctagaa ttctatgtta
      661 atcttttcac ccagcttaaa agcgctttcc ccaacatcag gctgcacgca cttgggccac
      721 cagagatagc gcatatagca aaaatagatg ggctatccaa cagtgaagtt ctgcaaaaac
      781 tagtggaatc ggggttggac agcctgcctg gtgcaggagc tgagattctg gttgaccggg
      841 taaggaaaag aatttcgccc ggcaaacccg atagccagtc gtggctcgac gttatggccg
      901 aagcacataa aatggggctg gttacatccg ccaccatgat gattggacac gttgaaacca
      961 tcgaggagcg ggttgagcac ctcatcaagc ttagagatct tcaaaaccgt aaaccaacca
     1021 atgcccccgg gtttgttaac tttatcgtat ggacattcca aggcaagggc accatgctcg
     1081 aaaaggaggg catcaccccg cacgttaccg acgaggagta catccgcacc attgccatta
     1141 gcc
//
LOCUS       DAOOQX010000717         1141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_176140_length_1141_cov_3.290055, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000717 DAOOQX010000000
VERSION     DAOOQX010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176140_length_1141_cov_3.290055"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLX53_09760"
     CDS             <1..302
                     /locus_tag="PLX53_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HOY73409.1"
                     /translation="ISTISRVANSKYIQTHFGIYPLKYFFSEGLMNDAGEEVSTREIK
                     KILQECILNEDKGNPLTDEELMEALKQKGYPIARRTIAKYREQLGIPVARLRKQL"
     gene            312..917
                     /locus_tag="PLX53_09765"
     CDS             312..917
                     /locus_tag="PLX53_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73410.1"
                     /translation="MTFKPDRWLSILLHPLFMMCYAFLTLYLSASYISFLPDSYKRLL
                     FIALFLNTIIIPIFILMLLKRLGTVKSIELFHHRERVFPIVMVIIPYVFTLLLLIRLQ
                     VPEIFIKIVQGGILAMIIAATVSYWWKISLHMVGIGGVTGFIIAGLILHYFTSLNLLV
                     VVVVLSGLLASARLVRGDHCPSQVYTGYLTGLASILLVFLL"
BASE COUNT          310 a          259 c          225 g          347 t
ORIGIN      
        1 acatttccac aatatcacga gttgccaaca gtaagtatat ccaaacccat ttcggtattt
       61 atcccctgaa atactttttt agcgaaggcc ttatgaacga tgcgggcgaa gaggtttcaa
      121 cccgagaaat taagaaaata cttcaggagt gcatccttaa tgaggataaa ggcaatcccc
      181 tcaccgacga ggagctgatg gaggcattaa agcaaaaagg atatcccatt gcacgccgga
      241 caattgcaaa gtacagggaa cagctgggca ttccggttgc ccgactaaga aaacaacttt
      301 aaaccttaaa catgactttt aaacccgacc gttggctttc cattctgctc cacccgcttt
      361 tcatgatgtg ctacgccttc ttaacgctgt acctatccgc atcatatatc tcatttctac
      421 cggattcata caaaaggtta ctctttattg ccctattttt aaatactatc attatcccca
      481 ttttcattct gatgctgttg aaacgcctcg ggacggtcaa aagcattgaa cttttccacc
      541 accgcgagcg ggtttttccc attgtcatgg tcataattcc ttacgttttt acgctgttgc
      601 tgcttatccg ccttcaggtt ccggaaatct ttatcaaaat tgttcaggga ggcattctgg
      661 ccatgattat tgcagcaacc gtatcctact ggtggaaaat aagccttcac atggtaggta
      721 ttggaggcgt taccggattc ataattgccg ggctgattct ccactacttc acctccctaa
      781 atctgctagt tgtggttgta gttctatcgg gtttattagc cagcgccagg ctggtaagag
      841 gtgatcattg tccttcacag gtttatacag gctaccttac cggattggcc tctattttgc
      901 tggtattctt gctttaatag ccatgcccga cgagtaaccg atttcgggca agggtgttat
      961 ccaaattata atatagttgc ctgataaaca gtaagttatt atttatggta agcggtttgt
     1021 agtacaacta attataagct caataaacct gctgcaaagt agatggctcg taataatttc
     1081 ttaaacatgc cgatcaggtt ttatggtgtt gaaagttcag catacccaaa aattattcaa
     1141 c
//
LOCUS       DAOOQX010000718         1140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_176433_length_1140_cov_2.799078, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000718 DAOOQX010000000
VERSION     DAOOQX010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176433_length_1140_cov_2.799078"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PLX53_09770"
     CDS             <1..688
                     /locus_tag="PLX53_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73411.1"
                     /translation="PRYLLPTLIPMAINTGFYIDYLFRRFSKGLPRAEKLWVYFSFGL
                     LGFIALAYPFALTFILKTDILNYLHIAIISSILMLTCAFFILRGMKSFNFQITFYSTI
                     ALFTVVVVAIIPLSQNLFTNPAYKPASSALNVEAKYGVKTYRLNDIAPEIVWDFGKVI
                     PSLPKINENIQAPPEKQFGLLVARSDSTIVRSGFPNFEVKKVHTIDMNYRPRHRDRLI
                     RDYYVLRLKE"
     gene            727..1056
                     /locus_tag="PLX53_09775"
     CDS             727..1056
                     /locus_tag="PLX53_09775"
                     /inference="COORDINATES: protein motif:HMM:NF021468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HOY73412.1"
                     /translation="MANYRVKPEDIKELIPQKSYCYASEMITVDGMPVGYMYREKPED
                     DDDSGWRFYSGAETEEYVEDVYHFMMFDLNYIANCDATIIPYLNSRVGTELERVEGTD
                     KFQPVED"
BASE COUNT          340 a          260 c          231 g          309 t
ORIGIN      
        1 accccgttac ctgctaccca ccctaattcc aatggctatc aacacaggct tttacataga
       61 ttatcttttt cgaagattca gcaaaggttt gccaagagcc gagaagctgt gggtttactt
      121 ttcatttgga ctattaggat tcatcgctct ggcataccct tttgccttaa cattcatcct
      181 taaaactgat atactaaact acctacatat tgccatcatc tcgtcaatac tcatgctaac
      241 atgcgcattt ttcattctca gggggatgaa aagttttaac ttccaaatca cattctactc
      301 cactattgcc ctttttacag tggttgtggt ggctataatt ccattatcgc aaaatttgtt
      361 taccaatcca gcctataagc ctgcaagcag tgcattgaat gtagaagcca agtatggcgt
      421 aaaaacctac cggctaaacg acattgcccc cgaaattgta tgggattttg gcaaggtcat
      481 tccttcccta cccaaaatta acgaaaacat acaagctccg ccagaaaagc agtttgggct
      541 gcttgttgcc cgaagcgatt caaccatagt taggtcaggc ttcccgaatt ttgaagtaaa
      601 aaaagttcac accattgaca tgaactatag accccgacat agggacaggc ttattagaga
      661 ctactacgta ttgaggttga aagaataacg gtaatcaaat aaattgcaaa agttaaacct
      721 aaagctatgg caaactacag ggttaaaccc gaggacataa aggaactaat tcctcaaaag
      781 tcttactgct acgcctcgga aatgattaca gttgatggaa tgcctgttgg atacatgtat
      841 agggagaaac ccgaggatga tgacgatagc ggctggcgtt tctactcggg tgccgaaacc
      901 gaggagtacg ttgaagacgt ttaccacttc atgatgtttg acctcaacta cattgccaac
      961 tgcgatgcca ccattattcc ctaccttaac tcaagggttg gcactgagct ggagcgggtt
     1021 gagggaaccg ataagtttca accggtagag gattagctac cacccagaag ctgtttagcc
     1081 atgtcggaaa tagctaaaca gcttctgggt ggtagctaat cctctaccgg ttgaaactta
//
LOCUS       DAOOQX010000719         1139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_176780_length_1139_cov_2.285055, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000719 DAOOQX010000000
VERSION     DAOOQX010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176780_length_1139_cov_2.285055"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLX53_09780"
     CDS             complement(<1..649)
                     /locus_tag="PLX53_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOY73413.1"
                     /translation="MKLDEIVSYFEELAPTAYQEEYDNAGLIVGDASMEVKAALLTLD
                     VTPEVVLEAIDKGANLIISHHPIIFRGIKKFTGSSNVEKTVAAAIKNDIAIFAAHTNF
                     DSMMGGVNLSIARKLGITDLEILQPINNGLFKLVTFVPVDSVEKVRDAMFKAGAGQIG
                     GYDCCSFGSEGFGTFRAGDNTHPYVGQKGEMHREPEVRVETILPKNILARVVEKMI"
     gene            complement(677..1015)
                     /locus_tag="PLX53_09785"
     CDS             complement(677..1015)
                     /locus_tag="PLX53_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOY73414.1"
                     /translation="MPYKEKNIEKFYFSIGEVAEMLGVNASLIRFWEKNFDIIQPRKN
                     QKGTRFYTKEDIDNLKLIYHLVKEQRMTIEGARKKIAEDKHSVSDSFKIIESLNSIKQ
                     MLLEVRDQLD"
BASE COUNT          280 a          258 c          256 g          345 t
ORIGIN      
        1 caatcatctt ttccaccact cttgcaagga tattttttgg aagaatggtt tccaccctaa
       61 cctcgggttc cctgtgcatc tcgccctttt ggcccacgta ggggtgagtg ttgtcgccag
      121 cgcggaatgt cccaaagccc tcggagccga aggaacagca atcgtaacct ccaatttgac
      181 cggctccggc cttaaacatg gcatctctaa ccttttccac gctatcgaca gggacaaagg
      241 ttaccagctt gaaaagccca ttgtttatgg gctgcaaaat ctccagatcg gtaataccca
      301 acttacgggc aattgacaag ttaaccccac ccatcatgct gtcgaagttg gtgtgggctg
      361 caaaaatggc aatatcgttc ttgatggctg ctgctactgt tttttcaaca ttgctgctgc
      421 cggtaaactt tttaataccc ctaaaaataa taggatgatg tgagattatc aggttagctc
      481 ctttatcaat ggcctcaaga acaacctcgg gggtaacatc aagagtgaga agagctgcct
      541 ttacttccat tgaggcatcg cctacaataa gaccagcgtt gtcgtattcc tcctggtagg
      601 ctgttggggc aagctcttca aaatacgaaa caatttcgtc gagtttcatt tgggtttgaa
      661 gacttgaaga atttggctaa tcgagctgat cgcgaacttc cagtagcatt tgcttgatgg
      721 agttcaggct ttcgataatc ttaaagctat cggatacgga atgtttgtcc tcggcaattt
      781 ttttcctcgc cccctcaatg gtcatccttt gctcctttac cagatggtaa attagcttaa
      841 ggttgtcaat atcttccttg gtgtagaatc tcgtaccctt ctgattctta cggggctgaa
      901 taatgtcgaa gttcttttcc cagaacctga taagggaagc gttaacgccg agcatttcgg
      961 ctacctctcc aatggaaaag tagaattttt cgatattttt ttccttgtaa ggcattagtt
     1021 caaaagaatc gtttagtcaa agctttgggt tggctgggat gctatttcaa ccagcttatc
     1081 gtactgctcg ggtgtaaggt cgttgaagta gtagttaatt gggttaaccg gcgtgttgt
//
LOCUS       DAOOQX010000720         1137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_177222_length_1137_cov_2.974122, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000720 DAOOQX010000000
VERSION     DAOOQX010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177222_length_1137_cov_2.974122"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..>1137
                     /locus_tag="PLX53_09790"
     CDS             28..>1137
                     /locus_tag="PLX53_09790"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOY73415.1"
                     /translation="MNENEKPSNLNFIGVVGIADPVRLDAAEAVAQCHRAGVDVKMLT
                     GDNIVTATSIGKQVNILSGNAEANVISGPDFEALSEDEARRMANQLKIMYRARPSDKM
                     RLVRLLQENGQVVAVTGDGTNDAPSLNRADVGLAMGSGTQVAKEASDIILPDDSFSSI
                     VNAIRWGRTLYGNIQKFLFFQLTINLLAVVIVLTGPFTGITLPLTVTQMLWVNLIMDT
                     FAALALATEQPHAGVLNKPPRSPKEFIVTPSIFKGILLASASFLIILLGMLIFFGRDG
                     NVSSYELTLFFNLFVMLQFWNLFNSRSLGDYSSALKGLFHNKGFITIAFVILLGQVVI
                     IQWGGEIFRTVPLSLPDWVKIFVASSLVLWIPEAYR"
BASE COUNT          286 a          260 c          261 g          330 t
ORIGIN      
        1 gcttataggt ttctagacca tgaggttgtg aatgaaaacg aaaagccatc caatcttaat
       61 tttattgggg ttgtgggtat tgccgatcct gttcgcctcg acgcagctga agcagttgcc
      121 caatgccaca gggccggggt tgatgtaaag atgctaacgg gtgataatat tgttaccgca
      181 acatcaattg ggaagcaagt aaacattctc tccggtaatg ccgaagcaaa tgtcatttca
      241 ggccctgatt ttgaggcact ttccgaagat gaagcccgca gaatggcaaa ccagcttaaa
      301 ataatgtaca gggcacgtcc ttcggataaa atgaggctag tcaggttgct acaggaaaac
      361 gggcaggtag tggctgttac aggtgatgga actaatgatg cgccctcgct taaccgagcc
      421 gatgtggggt tggctatggg gtcaggaaca caagttgcca aggaggcaag cgatatcatt
      481 ctgcctgacg attcgttttc cagcattgta aacgccattc ggtggggtag aaccctttat
      541 ggtaatattc agaagttcct cttcttccag ctcacaatca accttttagc agttgtaatt
      601 gtgctcaccg gcccctttac cgggattacg ctccctttaa cagtaaccca aatgctttgg
      661 gtgaacctca ttatggacac ctttgccgct cttgccctgg ccacagagca accccatgca
      721 ggcgtactaa ataaacctcc acgaagccca aaggaattta tcgtaactcc atcaattttt
      781 aaagggattc tactagcttc tgcatcattt ctgattattc tgttaggtat gcttatcttc
      841 ttcggccgcg acggcaacgt tagcagctat gagcttaccc tctttttcaa cctctttgtg
      901 atgcttcaat tttggaacct attcaactcc aggtcgcttg gcgattactc ttctgctctc
      961 aagggactgt tccataataa gggattcatt accattgctt ttgttatact tttaggtcaa
     1021 gtcgtaatta ttcagtgggg tggagaaatt ttcagaacgg ttccgcttag cctacccgat
     1081 tgggtaaaaa tatttgtggc ctcctcttta gtgctttgga ttcctgaggc atacaga
//
LOCUS       DAOOQX010000721         1136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_177508_length_1136_cov_3.086031, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000721 DAOOQX010000000
VERSION     DAOOQX010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177508_length_1136_cov_3.086031"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLX53_09795"
     CDS             complement(<1..427)
                     /locus_tag="PLX53_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOY73416.1"
                     /translation="MLKIQIQEDIKSYLGYTPTSGQAAAIDILSDFTLNPDSGKCLLI
                     KGYAGTGKTSLIAGYVKTLQKLKVPFELLAPTGRAAKVLTDYSGIKALTIHKCIYRQK
                     SISEGIGKFVLNLNKLTNAIFIVDEASMISNSSFEDSAFG"
     gene            655..>1136
                     /locus_tag="PLX53_09800"
     CDS             655..>1136
                     /locus_tag="PLX53_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOY73417.1"
                     /translation="MLKPLKLIDESFDRDRTGSYDLSVTISINGFSFAVLDTLRNTFI
                     VLVSTPKSEQQDFEELFNQSMVDYPWLAGSFKHVYLSFFSPYFTPVPGKFFSEQKARQ
                     LFELVHPLPDGFELHFTPNCNHTDATLLFALPNSVANWWLKLHPKASFLHPIAPLLSS
                     P"
BASE COUNT          294 a          253 c          226 g          363 t
ORIGIN      
        1 acccgaaggc gctatcctca aatgaggagt tggaaatcat tgatgcctca tctacaatga
       61 aaatggcgtt agtaagcttg tttaagttga gcacaaattt gccgataccc tcggatatac
      121 ttttctgccg gtatatgcat ttgtgaatgg ttagtgcctt gatgcccgaa tagtcagtaa
      181 gaacctttgc tgcccttccg gttggggcta gcagctcgaa tggcaccttc agtttttgca
      241 gagtctttac ataaccggct atcagggatg ttttacctgt tcccgcataa cctttaataa
      301 ggagacattt cccagaatct gggtttaggg tgaagtccga aaggatgtct atagcggctg
      361 cctgcccgga tgttggtgtg tatcctaaat aggacttgat atcttcctga atttgaattt
      421 ttaacaccgc ggtactacat tttctgattt aaaaggttta atttggcttt atgtttttaa
      481 atttgcgcaa acttatacta attacatctt tttgaaaaac ttttctttag gaataatcat
      541 tcatgcaatc atcctttcat accaggaaag agagtatgaa gatttttttg gttccgaaga
      601 agcatattaa tacacacctc tttggaggca ctttatcaaa caaggcaaat aggcatgttg
      661 aaacccctga agcttataga cgaatcgttc gacagggata gaacgggcag ctacgattta
      721 tccgttacca tttccataaa tggcttttca tttgctgttt tggataccct acgaaatacc
      781 ttcattgttt tggtttcaac acccaaaagt gagcagcagg attttgagga gctgttcaac
      841 cagtccatgg tggattatcc gtggttggcc gggagcttca aacatgttta cctttctttc
      901 ttctcaccat actttacgcc tgttccaggg aaattctttt ccgagcaaaa agccaggcaa
      961 ctttttgagc tagtacatcc cttgcccgat ggtttcgagc tacatttcac cccaaattgc
     1021 aaccatactg atgccaccct actttttgcc cttcccaatt ctgtggccaa ctggtggttg
     1081 aagctacatc caaaggcatc atttcttcac ccaattgctc ccctgcttag ttcacc
//
LOCUS       DAOOQX010000722         1132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_178657_length_1132_cov_2.734448, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000722 DAOOQX010000000
VERSION     DAOOQX010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178657_length_1132_cov_2.734448"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PLX53_09805"
     CDS             <1..485
                     /locus_tag="PLX53_09805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOY73418.1"
                     /translation="SGSVIGENCNIGQNVVVSPGVTLGRNVKVQNNVSIYSGVTCEDD
                     VFLGPSMVFTNVINPRSAINRKNEYMPTIVKRAATIGANATIVCGVTLGEFCFVGAGA
                     VVTKDVKPYALVVGNPARQIGWMSEFGHRLEFNSDNLATCPESGQVYRLDNNQVNKIS
                     "
     gene            489..>1132
                     /locus_tag="PLX53_09810"
     CDS             489..>1132
                     /locus_tag="PLX53_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOY73419.1"
                     /translation="MKNFALIGAAGFVAPRHLKAIKETGNTLLAALDKHDCVGIMDSY
                     FPQADFFVEYERFDRHIDKLKRQGIKVDYVSICTPNYLHDSHIRFALRQGADAICEKP
                     VVLNPWNIDALSEIEHETGRHIYTILQLRLHPAIIEFKKKIDNGPKDKIYDVDLTYIT
                     SRGRWYFISWKGDVQKSGGVATNIGVHFFDMLTWIFGDVKSGVVHMSMPDKAAG"
BASE COUNT          301 a          238 c          287 g          306 t
ORIGIN      
        1 tgtcgggttc ggtaataggt gagaattgca atattggcca aaacgtggtg gtgtctcccg
       61 gtgttaccct gggccggaat gttaaagtgc agaacaacgt atcaatttat tcaggggtta
      121 cctgtgagga tgatgttttt ttaggcccct caatggtttt tacaaatgtg ataaatcccc
      181 gcagcgccat caaccgaaaa aatgagtaca tgcccacaat tgttaaacga gcagccacca
      241 ttggtgctaa tgccactatt gtttgtggcg ttaccttggg tgaattttgc tttgtagggg
      301 caggagcggt tgtaaccaaa gatgtgaaac cctatgcgct ggtggtggga aatcctgcca
      361 ggcagattgg ctggatgagc gagtttggcc acaggctgga attcaatagc gataaccttg
      421 ctacctgccc tgagagcggg caggtttacc ggttggataa taatcaagtt aacaaaatta
      481 gttaaaaaat gaaaaacttt gcattgattg gcgctgcagg ctttgtggcg ccacgccatt
      541 tgaaggccat aaaggaaact ggaaataccc tgctggcagc cctcgacaag cacgactgtg
      601 tgggcataat ggacagctac tttcctcaag ccgatttttt tgtggagtat gagcgcttcg
      661 atcgccacat tgataagctt aaacggcagg gcataaaagt tgactacgtg agcatttgca
      721 cccctaacta cctccacgac tcccacataa ggtttgccct gcgacaggga gccgatgcaa
      781 tatgcgagaa acccgtggtt ctgaacccat ggaatattga tgcccttagt gaaattgagc
      841 atgaaaccgg caggcatatc tacactattc ttcagcttag gcttcatccg gccattattg
      901 agtttaaaaa gaaaattgac aatggcccca aggataaaat ttacgatgtt gacttaacct
      961 acatcaccag ccgtgggcgt tggtatttca tttcgtggaa gggcgacgtt caaaaatctg
     1021 gtggagttgc caccaacatt ggagtgcact tttttgatat gcttacttgg atttttggtg
     1081 atgtcaaaag tggcgttgtt catatgtcta tgcccgataa ggctgccggt ta
//
LOCUS       DAOOQX010000723         1129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_179517_length_1129_cov_2.308194, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000723 DAOOQX010000000
VERSION     DAOOQX010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179517_length_1129_cov_2.308194"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..801
                     /locus_tag="PLX53_09815"
     CDS             232..801
                     /locus_tag="PLX53_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOY73420.1"
                     /translation="MNADQQTSEQFYWLVRNRQSDRAYSNRPVDREILVRVVEAARLA
                     PSACNAQPWKFIVVDDPELKNRLADAAASKILGINHFTKQAPVHIVIVLEGANLNSNF
                     GSMVKGKTLPLIDIGIAAEHICLAAKSEGLGSCILGWFDEKAVKQLLDIPKSKRVPLI
                     ITLGYPKTEDIREKRRKPLEKIISFNGYS"
     gene            complement(806..>1129)
                     /locus_tag="PLX53_09820"
     CDS             complement(806..>1129)
                     /locus_tag="PLX53_09820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY73421.1"
                     /translation="LGLSLEEDLGLNYRLALPNKIFDYIASGVPILVSDLPEMRKIVE
                     TYHVGLVANTRDPEKLAGIVADFVENKKTREVMVNNVLAASETLCWDNEYPVLLNAVG
                     KAIGC"
BASE COUNT          319 a          265 c          248 g          297 t
ORIGIN      
        1 catctttcaa tattaagcct gcttgcgatt tcgtagcttt gccatgttca tattacacat
       61 caagttcaac gttggcttgg cagccaatct actttttact atcttcgacg tccgaattag
      121 aatgggcagc ttgaaggttt ttgaaatttc aacctgcata aatttccacc ttaatgaagc
      181 ttatagcttg taaaagacaa tgtttttaat cctttctaaa caactcctac catgaatgcc
      241 gaccaacaaa ctagcgaaca attctactgg cttgttcgaa acaggcagag cgacagggct
      301 tacagcaacc gaccggttga tagggaaata ctggtgaggg tagtggaagc agcccgtttg
      361 gctccatcgg cttgcaatgc tcaaccgtgg aagtttattg tggttgatga tcctgagcta
      421 aaaaacaggc tggccgatgc agcagccagc aaaatactgg gtattaacca ttttaccaag
      481 caagcgcccg ttcacattgt tattgtgctg gagggagcta acctgaactc caactttgga
      541 agcatggtaa aggggaaaac actaccgctt attgacattg gcattgctgc cgaacatatt
      601 tgcctggcag ctaaatcgga aggtttgggg agctgcatac tgggttggtt cgacgaaaaa
      661 gccgttaagc agctactgga tatacccaag tcaaaacgcg ttccgctcat catcacgcta
      721 ggctatccaa aaaccgagga cataagggag aagcggagaa agccgttgga aaaaattata
      781 tcatttaacg gctacagcta actgtttaac agccaatcgc ttttccaaca gcattcagga
      841 gaaccggata ctcgttgtcc cagcatagtg tttcagaggc cgctaataca ttattaacca
      901 tgacctccct ggtcttcttg ttttcaacaa agtcggctac aatgcccgcc aacttctccg
      961 gatctcttgt gttggccact agtcctacat gataggtctc aacaattttt cgcatctcgg
     1021 gtaaatcgct aaccaggatt ggcacgcctg aggctatgta gtcgaaaatc ttatttggca
     1081 gagcaaggcg gtagttcaac cccaaatcct cctcaagcga taagccaag
//
LOCUS       DAOOQX010000724         1128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_179715_length_1128_cov_3.019571, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000724 DAOOQX010000000
VERSION     DAOOQX010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179715_length_1128_cov_3.019571"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1128)
                     /locus_tag="PLX53_09825"
     CDS             complement(<1..>1128)
                     /locus_tag="PLX53_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73422.1"
                     /translation="FKNDAGAEVATGVYHASATFMGRGPTNFPVYDVEQCDGVITSSI
                     RPFRKLNATIYWDDTYISRWGDETGHGLMDETQRKQLKVSTIIPRIWSWNANLENTNF
                     NGNLNTMNTWFNAIDLGYSDITIHVNQSTTQCINGLAPWVGDIYKQGLPNNTITFTQA
                     DFTTKFFDPIEDPLTRIRIKSLPANGSLKLSGTAVTVNQEITLANLANLTFTPNTGWV
                     GITQFDWEAKNSQFWSMNADKVYITVNTPPTISSIADQNLCTNNPSPAIPFTVNDSET
                     PAANLTVTAYSHDPTFVPNSSITIGGSGTNRTISVTPVANKSGHAIIYVMVDDGLSQT
                     IQEFAVYVGPDLEFSGDTTICVGQDLYLVAQEVGASSYTWK"
BASE COUNT          274 a          239 c          298 g          317 t
ORIGIN      
        1 tacttccatg tataggatga ggcacctacc tcttgagcca ccaggtacaa atcctgacca
       61 acacaaatgg tggtgtcccc gctaaattcc aaatcggggc caacatatac agcaaattcc
      121 tgaatggttt gcgatagccc gtcatcaacc ataacgtaaa taatggcatg gcccgatttg
      181 ttggctacag gggtaacact aatggtacgg ttggttccgc taccgccaat ggtaatggag
      241 ctgtttggaa caaaggttgg gtcgtgagag tatgccgtaa cggtaaggtt ggctgcggga
      301 gtttcgctat cgtttaccgt aaaaggtatg gccggggagg ggttgtttgt acacaggttt
      361 tggtcggcaa tggatgatat ggtaggaggt gtgtttacag tgatgtaaac cttatcggcg
      421 ttcattgacc agaactggct attctttgcc tcccagtcga actgggtaat gccaacccat
      481 ccggtatttg gggtaaaggt taggttggcc aggttggcca gagtgatttc ctggttcaca
      541 gtaactgctg ttcctgacag cttaagcgag ccatttgccg gtagcgattt tatacggatt
      601 cgggtaaggg gatcctcaat aggatcaaaa aatttagttg taaaatcagc ttgtgtaaag
      661 gttatagtat tgttgggtag tccctgctta taaatatctc caacccatgg tgctaacccg
      721 ttgatgcact gggtagtgct ctggttcacg tgaatggtga tatcggaata tcccaggtct
      781 atagcgttga accatgtatt catggtattt aagttgccgt tgaagtttgt attttcaagg
      841 ttcgcattcc acgaccatat acggggtatg atggtgctaa ccttaagctg cttacgctga
      901 gtttcgtcca taagcccatg tcctgtttcg tcgccccatc gggagatata ggtatcgtcc
      961 cagtatatgg tagcgttaag cttcctgaag ggtcggatgg aagaggtgat taccccatcg
     1021 cactgctcca catcgtaaac aggaaaattt gttgggcctc tacccataaa ggtggccgaa
     1081 gcatggtaaa cacctgttgc cacctcggcg ccggcatcat tcttgaag
//
LOCUS       DAOOQX010000725         1128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_179752_length_1128_cov_2.615098, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000725 DAOOQX010000000
VERSION     DAOOQX010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179752_length_1128_cov_2.615098"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1128)
                     /gene="rpoB"
                     /locus_tag="PLX53_09830"
     CDS             complement(<1..>1128)
                     /gene="rpoB"
                     /locus_tag="PLX53_09830"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152451.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOY73423.1"
                     /translation="IGKITPKGESDPTPEEKLLRAIFGDKAGDVKDASLKASPSLYGV
                     VVDKMLFSRAIKDEKDQKKSKSTEKSKLEKIDNEFNRQVAELQTKLVDKLFVLVNGKT
                     SQGVVDNFNQELIPRGVKFTHKMLTEIDYMNVNPNKWTTDKAKNELIKTVIQNYILKW
                     KEIDAEFKRKKFNVTIGDELPAGIMQLAKVFIAKKRKLKVGDKMAGRHGNKGIVAKVV
                     RDEDMPFLEDGSPVDIVLNPLGVPSRMNLGQIYETVLGWAGQKLGVSFATPIFDGATL
                     DDITVYTEKAGLPRFGKTYLYDEGTGERFDQPATVGVIYMMKLGHMVDDKMHARSIGP
                     YSLITQQPLGGKAQFGGQRFGEMEVWALEAFGASHILQEILT"
BASE COUNT          275 a          295 c          233 g          325 t
ORIGIN      
        1 gtgagtattt cctgaaggat gtgggaagcc ccaaaagctt ccagcgccca tacctccatt
       61 tcaccaaacc tttgaccgcc aaactgagcc ttgccaccaa gtggttgctg ggtaataagg
      121 gagtaagggc ctatagaacg ggcatgcatc ttatcgtcaa ccatgtgtcc gagcttcatc
      181 atgtagatta cacctacagt tgcaggttga tcaaagcgct cgccggtacc ctcatcgtac
      241 aggtatgttt taccaaagcg gggtaagccg gccttctcgg tgtacaccgt tatatcgtca
      301 agagttgcac catcgaaaat cggtgtggca aagctaacgc ccagcttttg ccctgcccaa
      361 cctagaacgg tttcgtaaat ctgacccagg ttcattcggg agggaacgcc tagagggttc
      421 aaaactatat cgacaggcga cccatcctcc aggaacggca tatcctcatc acgaaccacc
      481 ttggccacaa ttcccttatt tccatgtcgg ccagccatct tatcgcccac ttttaacttg
      541 cgttttttgg caataaatac tttggctagc tgcataattc ctgcaggcag ctcatctccg
      601 atggttacat taaacttctt gcgcttaaac tccgcatcaa tctccttcca cttcagaatg
      661 taattctgaa taacagtttt aatcagctca ttcttagcct tatcggttgt ccatttgttt
      721 gggttaacat tcatgtagtc aatctcggta agcattttat gtgtaaattt caccccacga
      781 ggaatgagct cctggttgaa attatccaca acgccctgcg aggttttccc gtttacaaga
      841 acaaacagct tatcaaccag cttggtttga agctcggcaa cctgacggtt aaactcgttg
      901 tcgatttttt ccagcttact tttctcagtt gacttggatt ttttctggtc cttttcatcc
      961 ttaatagccc ttgaaaaaag cattttgtca actaccacgc cgtaaagcga tggggaagct
     1021 tttagtgagg catccttcac atcgccagcc ttatctccga aaattgcgcg tagcagtttt
     1081 tcctcgggag tgggatcaga ctccccttta ggggtgatct tcccgata
//
LOCUS       DAOOQX010000726         1127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_180014_length_1127_cov_2.572761, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000726 DAOOQX010000000
VERSION     DAOOQX010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180014_length_1127_cov_2.572761"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..599)
                     /locus_tag="PLX53_09835"
     CDS             complement(72..599)
                     /locus_tag="PLX53_09835"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF010555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOY73424.1"
                     /translation="MKIFLIGFMASGKTTIGRELASALQYKFIDLDDFIESRHNQTIK
                     QLFEFKGEDYFRKVENEALKEVCDYKDDYVISAGGGTSCFYNNIDFMNKNGITVYLRM
                     EVATLVSRLIESKIDRPLLWGKTGDELANYILRVLDERKKFYNKAQLVVDGDNLNPAE
                     LAQLISAIVKEEKLL"
BASE COUNT          331 a          239 c          206 g          351 t
ORIGIN      
        1 actatgtttt atgcgaaaga atcatggggc ggttagaggg gttacccctt ctgccccatg
       61 ctaattttta gctacaacaa tttctcctcc tttacaattg ccgaaatcaa ctgggcaagt
      121 tcagcagggt taaggttgtc tccatcaacc accagctgag ccttgttgta aaactttttc
      181 cgttcgtcga gcaccctgag gatatagtta gccagctcat caccagtttt tccccaaagt
      241 aatgggcggt caatcttcga ttcaattaaa cgggaaacca gtgtagccac ctccattcgc
      301 aggtaaacgg ttataccgtt cttgttcata aagtcaatat tgttgtaaaa gcatgaagtg
      361 ccgccaccag ctgagatgac atagtcgtct ttgtaatcac aaacctcctt aagggcttca
      421 ttttcaacct tccggaaata gtcttctcct ttaaactcaa acagctgctt gatggtttgg
      481 ttatgcctgc tttcgataaa atcatctaaa tctataaatt tatactgcag tgcgctggcc
      541 agctcccgac caatagtcgt tttaccgctt gccataaatc caattaaaaa tattttcatg
      601 ttagtggttt tttaaatcaa cctaatcctt caaaaattgc tgcctaaatc tctataaaaa
      661 aatacaaaac agttcaagtt gaaaatataa gggttacaag ccgtctattt tacaagaaat
      721 gtgaaaaaaa ggttacaaat tgcatggtgc taccctcccg cttggcattg tttacggtta
      781 taacgtttat tcgaacctct aattcaagcc ttatggaaac ttgcagctaa gactaccccc
      841 tcgctgagct tgttgtaacc tcaaaaaatt tgattagatt ggcaatacaa tacaaatttt
      901 tgcgcaccaa cacctcaacc taaaaacatt tcgtattttt aagcggatat ttagctggtg
      961 tgcctttaaa aatataaaac ctttccggtt atgaaggtca agatgcgaat tggagtaaaa
     1021 atgttactct ttgtcatgtc aacctcggtg ctggtgatgc tggttattgg gctttttatt
     1081 ggttttcgaa taaataaaat tgcccgggac gatgcggtaa gaatagt
//
LOCUS       DAOOQX010000727         1125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_180523_length_1125_cov_3.670093, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000727 DAOOQX010000000
VERSION     DAOOQX010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180523_length_1125_cov_3.670093"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..>1125
                     /locus_tag="PLX53_09840"
     CDS             250..>1125
                     /locus_tag="PLX53_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOY73425.1"
                     /translation="MSTFQCDFLVIGSGIGGLSYALKVADYGKVILVTKAGIDDTNTS
                     MAQGGIAAVTYPPDTFEKHVNDTLICGDGYCNEEVVRMVASEAPAQINQLIQWGVKFD
                     RKPNGQYDLAREGGHSEHRILHHKDITGAEIQRALSAGVRQHPNIQVFENHFAVELIT
                     QHHLGRVIHRGDPDITCFGAYVLDQKTQAVHTFLGRSVVVATGGSGNIYHTTTNPRTA
                     TGDGIAMVYRAKGAVENMAFVQFHPTSLYHPGERPSFLISEAMRGYGAVLKTSDGKEF
                     MQKYDSRLPLAPRDIV"
BASE COUNT          292 a          267 c          278 g          288 t
ORIGIN      
        1 tgcatcacta caaggtgccg gttacgctgg gctctgatgc ccatgtggag caaaacatag
       61 ggcagtactt taccgatgcc attgccctgc tacggtatgt gggatataag gaagtttcaa
      121 ccttctccga gcgtaagcga agcgccattc gaatttaact aactatgaca cactagtcgg
      181 tttatttgta tttttacccg tttttttatc aatcattcag tactacaggt tagatgaaat
      241 ttaactacca tgtcaacctt tcagtgtgat tttttagtta ttggctcagg aattgggggc
      301 ttaagctatg ccctaaaagt tgcagattat ggcaaggtaa ttctggtaac taaagcgggg
      361 atagacgata ccaacacctc catggctcaa ggtggaattg cagcagttac ctaccctccc
      421 gacacttttg agaagcatgt aaacgatacc ttgatatgcg gcgatggata ctgcaacgag
      481 gaggtggttc gcatggttgc ctcagaggcc cccgcccaga taaaccagct aatccagtgg
      541 ggcgtaaaat ttgacagaaa gcctaacggg cagtatgatt tagcccgcga gggaggtcat
      601 tcggagcacc gaatacttca tcacaaggat ataaccggcg ctgaaattca gagggcgctg
      661 agtgctggag ttcgtcagca tcccaacata caggtttttg aaaatcactt tgccgttgaa
      721 ctcataaccc agcaccattt ggggcgggtt attcaccggg gcgatcccga cattacctgt
      781 tttggagcct atgtgctgga tcaaaaaact caagcggttc atacattttt aggcaggtca
      841 gtggtggtgg caactggcgg ttctggcaat atctaccaca caaccactaa tcccagaact
      901 gcaaccggcg atggaattgc catggtttac agggcaaagg gcgccgttga aaatatggct
      961 ttcgtgcagt ttcaccccac atccctctac cacccggggg aacgtccgtc ttttctcata
     1021 tcggaagcta tgcgaggcta cggtgcagtg ctcaaaacat ccgacggtaa ggagttcatg
     1081 cagaagtacg actctcgttt accgttggct ccacgcgaca ttgtg
//
LOCUS       DAOOQX010000728         1125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_180567_length_1125_cov_2.864486, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000728 DAOOQX010000000
VERSION     DAOOQX010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180567_length_1125_cov_2.864486"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="PLX53_09845"
     CDS             complement(<1..1078)
                     /locus_tag="PLX53_09845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOY73426.1"
                     /translation="MKPARLAITALLLQLFIVNAYSQEAQVIDQVVAVVGGEHILLSD
                     IEQELLRQKMEGSPTGENNRCNVLEQILIQKLLLTQAKIDSLKTNESSVEMEIDRRLK
                     YFTSQLGSEKALESYFNKPIYEVKNDLREAIGEQLLTQQMRQKIVDKVKITPAEVKSF
                     YKNIPTDSLPTVPEQYELQQIMIYPPSTEEAKLQVREKLLEIRNRILQGERFSTLAVA
                     YSEDRASAIRGGELGFRYREELVKAFADAAFSLKEGQISQIVETEYGFHIIQLIEKRD
                     NQVNVRHILMKPQFSGDMLAQAMSKLDSIATLIKADSLTFEAAAMRFSQDKKSFLNGG
                     LVINSYNNTSLFEKEQLQPADFYAI"
BASE COUNT          256 a          256 c          255 g          358 t
ORIGIN      
        1 taatggcgta gaagtcggca ggttgaagct gctccttttc aaaaagagaa gtattgttgt
       61 aggagttgat gaccagccct ccgtttagaa acgatttttt atcctgagaa aatctcatgg
      121 ctgcagcttc aaaggtcaag ctatcggctt taatgagggt ggcaatgcta tcgagctttg
      181 acatggcttg tgccaacata tcacctgaga attggggttt catcagaata tgcctaacat
      241 ttacctgatt gtcgcgtttt tcaattagct ggatgatgtg gaagccatac tcggtttcaa
      301 ctatttggct tatttgaccc tcctttaggc taaacgctgc atcggcaaag gccttcacca
      361 gctcctcgcg gtaccgaaag cccagctctc cgcccctgat tgccgatgcc ctatcctcag
      421 agtaggcaac ggccagagtt gaaaagcgct cgccctggag tatgcggttt ctgatttcca
      481 gtagtttttc cctcacctgt agctttgcct cctcggtaga gggtgggtaa atcataattt
      541 gttgtagttc atactgctcg ggaactgtgg gtaggctgtc ggttggtatg tttttgtaaa
      601 aacttttcac ctcagcgggt gttatcttca ccttatcaac aattttttgc cgcatttgct
      661 gggtaagcag ctgctcgccg atagcttcac gcaaatcgtt cttaacttcg taaatgggtt
      721 tgttgaagta gctttcaagc gccttctccg atcccagctg gctggtaaag tacttcaacc
      781 ttcggtcaat ctccatttct accgacgact catttgtctt taagctgtca attttggcct
      841 gggttaacag caatttctgg ataagaattt gctccagcac gttgcagcgg ttgttttcgc
      901 cggttggtga accctccatt ttctggcgaa gaagttcctg ctcaatatcg gaaagcagga
      961 tatgctcacc acctactact gctacaacct gatctattac ttgcgcttcc tgcgagtagg
     1021 cgttaactat gaaaagttga agtaacaaag ccgttattgc taatcgtgct ggtttcattt
     1081 gtagtattgt ttaatttcta atgagtagct tgacatttac ctttt
//
LOCUS       DAOOQX010000729         1121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_181647_length_1121_cov_3.195122, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000729 DAOOQX010000000
VERSION     DAOOQX010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181647_length_1121_cov_3.195122"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /gene="rsgA"
                     /locus_tag="PLX53_09850"
     CDS             <1..527
                     /gene="rsgA"
                     /locus_tag="PLX53_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOY73427.1"
                     /translation="AAQTAMMNQFISIYEEVGYKCLCTSATTGYNLDAFRQLLQGKTS
                     LISGNSGVGKSSLINAIEPTLQLKTSSISAAHLKGKHTTTFSEMFELSFGGYVIDTPG
                     IKGFGLIDIDKKELYHYFPEIFRLKDKCRFYNCTHVHEPGCAVIEAVESGTISQSRYM
                     SYLSIYFDENSKYR"
     gene            573..>1121
                     /gene="mnmH"
                     /locus_tag="PLX53_09855"
     CDS             573..>1121
                     /gene="mnmH"
                     /locus_tag="PLX53_09855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016785 - selenotransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HOY73428.1"
                     /translation="MEVSSIHINSFLTSRESCPVVDVRTPDEFNRGHIPGAVNIPLFT
                     NEEREQVGTKYVRESRYKALLAGLDLAGPKLTSYLHQLKKVASGKNILVYCWRGGLRS
                     SSMAWLFNLAGYNSKTLAGGYKSYRRFAQQFFTNPYRFIVLGGMTGSGKTELLNRLKT
                     INEQVIDLEALANHKGSAFGWIG"
BASE COUNT          329 a          262 c          249 g          281 t
ORIGIN      
        1 acgctgctca aactgcaatg atgaaccagt ttatttcgat ttatgaagaa gtaggttaca
       61 aatgcctgtg cacctcagcc acaaccggtt acaaccttga cgcattcagg cagcttcttc
      121 agggtaaaac cagcttgatt tccggaaatt cgggtgtggg aaaatcaagc ctgatcaacg
      181 ccattgaacc gacactccag ttaaaaacca gctcaatatc cgcagcccat cttaagggca
      241 agcacaccac caccttcagc gaaatgtttg agcttagttt tggcggatat gtgatagaca
      301 cccccggaat taagggtttt ggccttatag atatagataa aaaggagctt taccactact
      361 tccccgaaat tttcaggtta aaagataagt gccgtttcta caactgcaca catgtgcatg
      421 agcctggatg cgcggtgata gaagcagttg aatcaggaac aataagccaa tcgcgataca
      481 tgagctatct aagcatttac tttgacgaaa actcaaaata caggtagcag ctatattgtt
      541 tcaaaaaaca atatcaaaca ctttgccaca cattggaagt aagttcaatt catatcaact
      601 cctttttaac ctccagggaa agttgccctg ttgttgatgt tagaactccc gatgagttta
      661 accgaggtca tattcccggt gcagtaaaca tccctctttt taccaacgag gagcgggaac
      721 aggttggaac aaaatacgtc agggagagcc gctataaggc tctactggcc ggccttgatt
      781 tagctggacc caagctcacc tcatacctgc atcagcttaa gaaagtcgcc tccggtaaaa
      841 atattctggt ttactgctgg cggggcgggc tacgtagctc cagcatggca tggcttttca
      901 acctcgctgg gtataactcc aaaacgctgg caggaggata caagtcgtac cgtaggtttg
      961 cgcaacagtt cttcactaat ccataccgat ttattgtttt aggtgggatg accggtagcg
     1021 gcaaaacaga actgctgaat cggcttaaga ccataaacga acaggttatt gacctggaag
     1081 ctctcgccaa ccataagggc tcagcattcg gatggattgg t
//
LOCUS       DAOOQX010000730         1113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_183967_length_1113_cov_1.844991, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000730 DAOOQX010000000
VERSION     DAOOQX010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183967_length_1113_cov_1.844991"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(355..>1113)
                     /locus_tag="PLX53_09860"
     CDS             complement(355..>1113)
                     /locus_tag="PLX53_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOY73429.1"
                     /translation="GRFRQQTYENGKRITPIVTMVTNFTAPTGNTPALLTWDEVTTLF
                     HEFGHALHGLFTDGPYDRIAGSLPRDMVELPSQVMENWAGEPRVIKVYAKHYQNGSPM
                     PEDLINRLKKSMTFNQGFETVEYIAASVLDLDWHSITEPQKVDVLQFENNSMKRIHLL
                     DEIYPRYRTTYFNHIIGGYAAGYYVYLWAAVLDTDAFNAFAETGDIFNHEVATRFRKH
                     VLTEGGNDEGMIQYVKFRGKEPSVEPLLKKRGFK"
BASE COUNT          273 a          231 c          280 g          329 t
ORIGIN      
        1 ctaccagcaa tgacaccgtt aacgcggagt atatgtttat ccttttcatt cgggttggtg
       61 ttgaaaattc aaaggtacta ctttttagga ttttttgggt agtcttaccg ataagtttgg
      121 gtagagtctg atgatgggtg gaggttttcc atgggttggt aatatctgtt tataagcttg
      181 ttaccgttac cttcttatct aaatcggcaa ttgtcgggct gtgaatggaa tttttgacca
      241 aaagtgcatg gtgggcagct gagctggatt tgaaaaaaaa aatgggctgc agcatttagc
      301 tacagcccat ttgacccaaa caaaccctta aatatgtaaa agcactccga aagactattt
      361 aaatcctctt tttttaagaa ggggttcaac cgagggttct ttccctctaa acttaacata
      421 ctgaatcatt ccctcatcgt ttcccccttc agttaatacg tgtttgcgga atctagtggc
      481 cacttcatgg ttaaaaatat caccggtttc ggcaaaggcg ttaaaggcgt cggtatcaag
      541 aacagccgcc caaaggtaaa cgtagtagcc ggctgcatac ccacctatga tgtggttgaa
      601 gtaggtggtg cggtagcgtg ggtatatctc atctagcaga tgaattcgct tcatggagtt
      661 gttttcaaat tgcagcacat caactttctg aggttcggtt atgctgtgcc aatccaagtc
      721 aagcaccgat gcagcaatat actccacggt ttcaaaaccc tggttaaagg tcatgctctt
      781 tttcaacctg ttgatgaggt cttcgggcat agggctgcca ttttggtaat gcttggcgta
      841 aaccttaata acgcggggct ctccggccca gttctccatc acttgtgagg gcagctctac
      901 catgtcgcgg ggtaggcttc cggcaatgcg gtcgtatggg ccatcggtaa ataagccgtg
      961 cagggcatgg ccaaactcgt ggaacagggt agttacctca tcccatgtga ggagggctgg
     1021 ggtgtttcca gtgggagcgg taaaattagt aaccatggtt acaattgggg taattcgttt
     1081 tccgttttcg taggtttgct gtcggaaacg gcc
//
LOCUS       DAOOQX010000731         1109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_184941_length_1109_cov_3.391841, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000731 DAOOQX010000000
VERSION     DAOOQX010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184941_length_1109_cov_3.391841"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLX53_09865"
     CDS             <1..369
                     /locus_tag="PLX53_09865"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOY73430.1"
                     /translation="NILDGYITYGKAVGTGVLVSLFASIIYAFYFIILTKFIDTGYMD
                     KVLETVEQAYAENGLSEDQIAAALEMSKKMMTPIMMALSTVFGFVFMGTLFSLITSIF
                     IKKEAPLFGSESKEGETLSE"
     gene            380..>1109
                     /locus_tag="PLX53_09870"
     CDS             380..>1109
                     /locus_tag="PLX53_09870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOY73431.1"
                     /translation="MDISIVVPLYNEEESLSELAAWINRVMTLHNLSHEVIMVDDGSS
                     DRSWAVIQELRKQYPAIRGIRFRRNYGKSAALQVGFTAASGDVVITMDADLQDSPDEI
                     PELFRMIKEEQLDLVSGWKKKRYDPLLTKRIPSKLYNFTVRMVSGIKLHDMNCGLKAY
                     RNNVVKSVEVYGEMHRYIPVLAKQAGFRKIGEKIVQHHERKYGSSKFGWSRFINGFLD
                     LLSVTFVSRFGKKPMHFFGTLGTLM"
BASE COUNT          286 a          263 c          292 g          268 t
ORIGIN      
        1 aacattctgg atggttacat tacctatggg aaagcagtgg gaactggagt tctggtttcg
       61 ctcttcgcat ccatcatcta tgccttctac ttcattattc tcacaaagtt tatagatacc
      121 ggctacatgg acaaggttct tgaaaccgtg gagcaggcat acgctgaaaa tggcctgagc
      181 gaagatcaga ttgcggcagc gttggagatg tcgaaaaaaa tgatgacacc gataatgatg
      241 gccttaagca ctgtttttgg ctttgtgttt atgggtaccc tcttctcgct gattacctcc
      301 atttttatta agaaggaagc cccgcttttc ggcagcgaga gcaaggaggg tgaaactctt
      361 tccgaatagc cctgaattaa tggacatatc tattgtagtt ccgctttaca acgaggagga
      421 atcgctgtcc gaacttgcag catggattaa ccgagtgatg accctgcaca acctgagcca
      481 tgaggtgatt atggttgatg atggcagcag cgacaggagc tgggcggtta tacaggagct
      541 ccgaaagcag taccccgcca ttaggggaat caggttcagg cggaactacg gtaaatcggc
      601 tgcactccag gttggcttta ccgcagcatc gggtgacgtg gtaatcacca tggatgccga
      661 ccttcaggac agtcccgacg aaatccccga gcttttcagg atgattaagg aggagcagct
      721 tgacctggtc tcgggttgga aaaagaaacg ctacgacccc ctgctaacca agcgaattcc
      781 cagcaagctg tacaacttta ctgtcagaat ggtgagcgga ataaagctgc acgacatgaa
      841 ctgcggccta aaggcatacc gcaacaacgt ggtgaagagc gtggaggtgt acggcgaaat
      901 gcaccgctac atccccgttc ttgccaagca ggcggggttt cgcaaaattg gcgaaaaaat
      961 tgtgcagcac cacgagcgca agtacggctc gtcaaaattt gggtggtcgc gatttatcaa
     1021 cggctttctc gacctgctct cggttacctt tgtctcccgc tttggcaaga aacccatgca
     1081 cttttttgga acactgggca cgctcatgt
//
LOCUS       DAOOQX010000732         1109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_184956_length_1109_cov_3.034156, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000732 DAOOQX010000000
VERSION     DAOOQX010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184956_length_1109_cov_3.034156"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1109
                     /locus_tag="PLX53_09875"
     CDS             <1..>1109
                     /locus_tag="PLX53_09875"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HOY73432.1"
                     /translation="TLQQEASRKYGFSVAQTMSIAQKLYEAGHITYMRTDSVNLSSTA
                     IAAARDVIVAEYGEKYNQPRQYKTKTKGAQEAHEAIRPTYLDRVKISGTSTEQKLYDL
                     IRKRTIASQMSDAQIEKTTINIDISGSHYKFVASGEVIVFDGFLKVYLESSDDEAEEN
                     GREILPPVKAGQTLAVMEIVANERFTQRPPRYTEASLVKKLEELGIGRPSTYAPTIST
                     IISRGYVAKEDRPGVERNYKSIILKNGKITRQEKVENSGAEKAKLFPSDIGMVVTDFL
                     LEYFRDILSYDFTAHIEEKFDEVALGKLEWTRMIDEFYKPFHSLVEKTIKESDYTKGE
                     RVLGIDPVSGKPVSVRIGRYGPVVQLGEADASKKT"
BASE COUNT          348 a          262 c          255 g          244 t
ORIGIN      
        1 ccaccttgca acaggaggct agtcgtaaat atggtttttc tgttgcgcaa acaatgtcga
       61 tagcccagaa actttacgaa gcaggccaca taacctacat gcgtaccgac tctgtaaacc
      121 tttcttcaac agctattgct gccgcaagag atgtaatagt agcagaatac ggtgagaaat
      181 acaatcaacc cagacagtac aaaaccaaaa ccaaaggtgc ccaggaagcg catgaagcca
      241 taaggccaac ctacctcgac agggttaaga taagcggaac cagcaccgag caaaaactct
      301 acgatttaat caggaaacga accatagcct cccagatgag cgatgcccaa attgagaaaa
      361 ctactataaa cattgatata tcgggatcgc attacaaatt tgttgcttcc ggcgaagtaa
      421 tagtgttcga cggatttctc aaggtttacc ttgaatcgtc cgatgacgaa gccgaggaaa
      481 acggacgtga aattcttccc ccggtaaagg ccggccaaac cctagcggta atggaaattg
      541 ttgccaacga acgttttacc caacgtcccc caaggtacac cgaggccagc ttggttaaga
      601 agctcgaaga gctgggtatt ggcagaccat caacctacgc accaactatt tccacaatca
      661 tttcccgcgg ctatgtggcc aaggaagaca gaccgggagt tgagcgaaac tataagtcca
      721 taatccttaa aaatggtaaa ataacccggc aggaaaaggt tgaaaactcc ggtgccgaaa
      781 aagccaaact ttttccaagt gatatcggta tggtggttac agatttccta cttgagtact
      841 tcagggatat cctcagctac gattttaccg cccacatcga ggagaagttt gatgaggttg
      901 cccttggcaa actcgagtgg acaagaatga tcgacgaatt ctataaaccc tttcactccc
      961 ttgttgaaaa aactatcaag gagtcggatt acactaaggg tgaacgtgtg cttggcattg
     1021 atccggttag cggtaaacct gtgagcgtcc gtataggccg atatggcccg gtggttcagc
     1081 tgggagaggc tgacgcttca aaaaaaaca
//
LOCUS       DAOOQX010000733         1106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_185849_length_1106_cov_3.251189, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000733 DAOOQX010000000
VERSION     DAOOQX010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185849_length_1106_cov_3.251189"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..>1106)
                     /locus_tag="PLX53_09880"
     CDS             complement(151..>1106)
                     /locus_tag="PLX53_09880"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOY73433.1"
                     /translation="LRAINYLSLSGTPVENSLLDLWSQLNFLNRGMLGGLRAFRNEFQ
                     TPIERQGDHEKEKQLKQLIEPLILRRTKEEVAKDLPEISEQVVYCRMSDSQKEIYEKE
                     KSVVRNFILETLDSPAPNQSTVSILASLTRLRQIANHPAMLPEYRLADSGKFDEVAMG
                     IESATAGHHKILVFSSFVRHLEQVEVFLQDRKIGYEKLTGSSANRSEIVERFQTDPDK
                     KVFLISLKAGGVGLNITAADYVFILDPWWNPAAEMQAASRSHRIGQDKKVFVYRFITS
                     ETVEEKILSMQERKENLAQLFVNSSSSLGQISREAILELLE"
BASE COUNT          261 a          275 c          253 g          317 t
ORIGIN      
        1 gggctctgat gtaatgaagc gtatcgagcg caacatactc aactttatta aaaccctggt
       61 ttagcaccaa ctcctgctcc agtagatttt tctgaaacca tgttaacctt caaccccttt
      121 gctgaatagc tttagcttgg ggttttatac ctattccagc agttcaagaa tggcttccct
      181 gctaatctgg cctagtgaac ttgacgagtt tacgaacagc tgggcaaggt tctcctttct
      241 ttcctgcatg gagagaattt tttcctccac agtttcggat gtaataaaac ggtaaacaaa
      301 aactttttta tcctgaccaa tcctgtgcga ccgcgatgca gcctgcattt cggcagcagg
      361 attccaccat gggtcgagaa tgaaaacata gtcggctgcg gtaatattca ggccaactcc
      421 acccgcttta agcgatatga ggaatacctt tttatccggg tcggtttgga acctttccac
      481 aatttcgctc cggttggctg atgaccctgt taatttctcg tatcctattt ttctatcctg
      541 aaggaaaacc tctacctgct caagatgtct gacaaatgag gagaacacca ggattttgtg
      601 atgacctgcg gtggcgctct cgatgcccat agccacctcg tcaaacttgc cggaatcggc
      661 cagccggtat tcgggcaaca tggccggatg gttggcaatt tgtctgagtc gggttaggct
      721 agccagaatg gaaactgttg actgattagg ggctggactg tctaaagttt cgagtatgaa
      781 gttgcgaacc accgattttt ccttctcgta aatctccttt tgtgaatcgg acattctgca
      841 gtaaaccacc tgctcgctaa tttcaggtag gtctttagcc acctcctcct tagtgcggcg
      901 caaaatgaga ggctcaataa gctgcttcag ctgcttttcc ttttcgtgat ctccctgccg
      961 ctcaatggga gtttgaaact cattacggaa ggcccttaag ccgcccagca ttcccctgtt
     1021 caggaaatta agctgcgacc acaggtcgag cagggagttt tcaacaggtg ttccgcttag
     1081 gctgagatag ttgatggccc tcagct
//
LOCUS       DAOOQX010000734         1105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_186174_length_1105_cov_2.806667, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000734 DAOOQX010000000
VERSION     DAOOQX010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186174_length_1105_cov_2.806667"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1105)
                     /locus_tag="PLX53_09885"
     CDS             complement(<1..>1105)
                     /locus_tag="PLX53_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOY73434.1"
                     /translation="LNLDEFAMGSTTKTSYFGFTKNPWKSKLHDKDLIPGGSSGGSAA
                     GVSAGLFMAATGTDTGGSIRQPAGLTGICGMKPTYGRCSRFGVVAFSSSLDHMGPMAR
                     TVYDCALMLENMAGYDAKDSTCAKLPVPKYTAAVERGVKGLKIGIPAEYDNELLSPEV
                     KEVWQNAQNILREAGAEIVPISMPATDAAVSTYYIIAPAEASANLARYDGVRYGYRAT
                     GEFKDIEELYMKTRSEGFGDEVKRRVLMGTEVLSSGYYDAYYNKACKVRRLIRNDFNN
                     AFTQVDAILVPTTTGEAYTLDAKMTLLEVYLNDVYTIPANMSGVPAMSVPAGNSPKSG
                     LPLGLQVMGKYFNEETVFAVARVIERETSATKLP"
BASE COUNT          318 a          270 c          213 g          304 t
ORIGIN      
        1 tggaagtttt gtggcagagg tttcgcgttc aataactctg gctacggcaa aaacggtttc
       61 ttcattaaaa tatttgccca taacttgtaa ccctaaaggt agccccgatt taggagaatt
      121 tcccgcaggc acgctcattg cgggcacccc tgacatattc gcaggtatgg tgtaaacgtc
      181 attcaaataa acttctagca atgtcatttt tgcatcaagg gtgtaagcct cacctgtagt
      241 tgtaggcact aaaatggcat ccacttgagt aaaagcatta ttaaaatcgt tgcgaattaa
      301 acggcgaact ttgcaagctt tattgtaata agcatcatag tatcccgacg ataaaacttc
      361 cgttcccatc aaaacacggc gtttaacttc atcaccaaaa ccttcggaac gggtcttcat
      421 ataaagttcc tcaatatctt taaactcacc tgttgctcgg tagccatagc gtacaccatc
      481 ataacgcgct aagtttgccg aggcttctgc cggggctata atataataag ttgacaccgc
      541 agcatcagta gctggcatcg aaataggaac aatttcggca ccagcttccc tcaatatatt
      601 ttgagcattt tgccaaactt ctttcacttc gggacttaaa agttcgttgt catattcggc
      661 aggaatgcca atttttaatc ctttaactcc ccgctccacc gcagcggtat atttaggtac
      721 gggcagtttt gcacaagtag aatctttagc atcataacct gccatatttt caagcattag
      781 ggcgcaatca taaaccgtgc gtgccattgg ccccatatga tcaagcgatg acgaaaaagc
      841 caccacccca aaacgtgagc aacgtccgta agtaggcttc attccgcaaa tacctgtaag
      901 tcctgcaggt tggcgaattg aacctcccgt atctgtacct gtggccgcca taaataaccc
      961 tgcggatact ccagcagccg aacccccaga cgaccctcca ggaattaaat ctttatcatg
     1021 cagtttagat ttccatggat ttttggtaaa cccaaaataa ctagttttgg tggtagaacc
     1081 catagcaaat tcatctagat taagt
//
LOCUS       DAOOQX010000735         1104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_186433_length_1104_cov_3.100095, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000735 DAOOQX010000000
VERSION     DAOOQX010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186433_length_1104_cov_3.100095"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..418)
                     /locus_tag="PLX53_09890"
     CDS             complement(32..418)
                     /locus_tag="PLX53_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOY73435.1"
                     /translation="MKKIIYWTSTVLISLMMLSSAWGYFTNPDMKGAFVHLGFPDYFR
                     IELGTAKFLGVLALLIPMIPARIKEFAYFGFTITFISAIIAHLSSGDPIMAVVFPLVA
                     QVLLTVSYIYFILISDKKMTMQEAES"
     gene            complement(441..674)
                     /locus_tag="PLX53_09895"
     CDS             complement(441..674)
                     /locus_tag="PLX53_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73436.1"
                     /translation="MEAQKKLRCPLGIPGGILVALIGLVGIVLNIINFDWFELLQSIA
                     LLLLGLPFVRVTMMVHLGNDRLDALEDKVNGKK"
     gene            complement(693..956)
                     /locus_tag="PLX53_09900"
     CDS             complement(693..956)
                     /locus_tag="PLX53_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOY73437.1"
                     /translation="MEKPVLIEMFYTLSCPNCRTLKRMLDEILPTFGNKFEFKRTMAN
                     SPVGMIRTMKLGIGAVPTLLINNEIVFRQVPTKEELINKLNQY"
BASE COUNT          341 a          225 c          207 g          331 t
ORIGIN      
        1 cagataacca gggcaacata tcattatttg cttagctctc ggcttcctgc attgtcatct
       61 ttttatcaga aatcaggatg aagtagatgt aggatacggt aagcagtacc tgggcaacca
      121 gcggaaaaac aactgccata attggatcgc cactcgacaa atgcgcaatt atggccgaaa
      181 taaaggtaat ggtaaaccca aagtaggcaa attccttaat tcgagcagga atcataggaa
      241 tcagcagggc taaaactccc agaaattttg ctgttcccaa ctctattctg aagtagtccg
      301 gaaatccaag gtggacaaac gctcccttca tatccggatt agtaaaatat ccccatgcac
      361 tcgacagcat catcaacgat atgagcacgg tgcttgtcca gtaaataatt ttcttcaccc
      421 tcataatatt tcttccttga ttatttctta ccatttactt tatcctcaag tgcatcaagc
      481 ctatcgttgc ccaaatgaac catcatggtt accctaacaa aaggtaaacc gagtagcagt
      541 aaggcgattg actgtagcag ctcaaaccag tcgaaattga taatgtttag aacaattccc
      601 accaatccga ttagggctac taatattccg ccgggaatac ctagtgggca gcgaagtttt
      661 ttttgtgctt ccattgtaat taaaatttaa ggttagtatt gatttagttt atttataagc
      721 tcctcttttg ttggaacctg cctaaaaaca atctcgttgt tgataagcaa tgttggaaca
      781 gcacctatac caagcttcat ggttcgaatc ataccaaccg gggagttggc catggtgcgc
      841 ttaaactcaa atttattccc aaaagttggg agaatttcat ccagcattcg ctttagtgtg
      901 cgacagttgg gacaagagag cgtgtaaaac atttcaatta aaacgggttt ttccattgtt
      961 ttcatttaaa tttttgtcat agtaacaagc caagtagtta tttgttgcaa catccattaa
     1021 tttttttgct tagattttga ttgcaaatgc aactttttag ggcgttagct taagggcaaa
     1081 cgaaaaaaaa ctaatttttc aaga
//
LOCUS       DAOOQX010000736         1104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_186645_length_1104_cov_1.522402, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000736 DAOOQX010000000
VERSION     DAOOQX010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186645_length_1104_cov_1.522402"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLX53_09905"
     CDS             <1..592
                     /locus_tag="PLX53_09905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY73438.1"
                     /translation="AFPLGEIPPRDVLAMYGIFEPYYLMVSRLTPENNIEMIARSFVA
                     LGENLIIIGRYDTRFGESLYRKFEHFKNIRFLGPNFNQDDLNALRYHSLAYIHGHTVG
                     GTNPSLLEAMACKCFVIAHDNPFNREVLGDEGWYFGSTTELNDRIREFGRLPAMSVLK
                     AKQSNYTRIKNLYSWEAVADSYEELFSSINSQQDQG"
     gene            complement(648..>1104)
                     /locus_tag="PLX53_09910"
     CDS             complement(648..>1104)
                     /locus_tag="PLX53_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY73439.1"
                     /translation="TYTFDAGAGFVSYLWSDTSTGQTLLVDGAVTGTGEFTYWVTVTN
                     EFSCSASDTAVVVVSPVGGVPMQISWEISIYPNPSSGEFKLKVSGIEPGTYTLTILNA
                     GGQIVYGSTFSASDTEITRAINIQTLSKGLYYVRFENTNKGFTRKIILK"
BASE COUNT          298 a          224 c          253 g          329 t
ORIGIN      
        1 tgcatttccg cttggagaga ttcccccacg ggatgtattg gcaatgtacg gaatctttga
       61 accatactac cttatggttt caaggctgac ccccgaaaac aacatcgaga tgattgctcg
      121 ctcttttgtt gctctaggcg aaaatctgat tattattggc aggtacgaca ctcgttttgg
      181 tgaaagttta tatagaaaat ttgaacattt caagaatatt cgttttttag gtccaaattt
      241 taaccaggat gatttgaatg ccttgcgtta ccattcattg gcctatatac atgggcatac
      301 ggttggaggc actaacccct cgttgctgga ggcaatggcc tgtaaatgtt ttgttatagc
      361 gcatgacaac ccttttaacc gggaggttct tggtgatgaa gggtggtatt tcggcagcac
      421 cacggagttg aatgacagga ttagggagtt tggccgtctt cctgccatgt ctgttttaaa
      481 ggctaagcag tcgaattata ctaggattaa aaacctatac agctgggaag ctgtggccga
      541 tagctacgag gagctatttt cttcaatcaa tagccagcaa gatcaaggtt aaatgtaaaa
      601 ggagggtaaa tgaaccctcc ttttatctat aaattcaagt aaagctatta tttcagaatt
      661 atttttcggg taaatccctt attcgtgttt tcaaatctta cgtagtacaa tcctttagat
      721 agggtttgaa tgttaattgc ccgtgtgatt tctgtatcac ttgcgctaaa ggttgatcca
      781 tagacaattt gaccaccggc atttaaaatt gtaagggtgt atgttccggg ttcaatgcct
      841 gaaaccttca gcttgaactc acccgatgac gggttggggt agattgaaat ttcccacgaa
      901 atttgcatag gaacgccacc cacaggtgaa acaaccacga ctgccgtgtc ggaagcagaa
      961 cagctaaact cgttggttac ggttacccag tatgtgaact cacctgttcc ggtaacggct
     1021 ccatctacca gaagggtttg tccggtagag gtatcgctcc aaaggtatga tacgaatcct
     1081 gcccccgcat caaatgtgta ggtt
//
LOCUS       DAOOQX010000737         1101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_187271_length_1101_cov_3.284895, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000737 DAOOQX010000000
VERSION     DAOOQX010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187271_length_1101_cov_3.284895"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PLX53_09915"
     CDS             <1..571
                     /locus_tag="PLX53_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73440.1"
                     /translation="RQQSLICSAYQQYLKNGGTYSFKRWLYSGDVFCPEHLYYDGLIS
                     YYDKLFGREMVKVYFYEDFKEDNIGFLTRLNREFSFHVDMNAISLKPENVGLRRRVVP
                     FLFMVNHFYKKPIGLKRYIIHVPGMTSVGKGIYKFINPLPLFGNFLSEDKMLKPKDKL
                     KLHDFYSESNRRLANRVGLERLNHHGYYL"
     gene            576..>1101
                     /locus_tag="PLX53_09920"
     CDS             576..>1101
                     /locus_tag="PLX53_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73441.1"
                     /translation="MEPINTILHIGYPKTATKWFQNEFYPRVNGIFFIHRKDVFKWLA
                     FQNIFDYSPVETLEHFVSTTDGKRLVICDEIILGGLDIGFGSGEYVCMMANRLKETFG
                     EASIIIFIRNQHSALESVYGHYIKSGGTYSVKKYMGIYRKFTRPFHDYHLFNPDLFQY
                     NKIVELYARLFGSEN"
BASE COUNT          331 a          209 c          231 g          330 t
ORIGIN      
        1 acgtcagcaa tcgttgattt gttctgccta tcagcagtac ttaaagaatg gaggaaccta
       61 cagttttaag cgatggcttt attccggcga tgttttctgt cctgaacatc tctactacga
      121 cgggttgata agttactacg ataagctttt tgggagggag atggttaagg tttattttta
      181 tgaagatttt aaggaagata atattggctt tttaacccgg ttgaacagag agttcagctt
      241 tcatgttgat atgaatgcca tttctctaaa acctgaaaat gtaggcctgc gacgaagagt
      301 agttccattt ctttttatgg taaatcattt ctataaaaaa cccatagggc taaagcgata
      361 cataattcat gtaccaggaa tgacctcggt agggaaagga atttataagt ttattaatcc
      421 cttgccacta tttggaaatt tcctttcgga agataaaatg ctaaaaccca aagataaatt
      481 gaagctacac gatttttaca gcgagtcgaa cagaagactg gccaacaggg ttggattgga
      541 aaggttaaac caccatggct attacttgta agcaaatgga accaataaac actattcttc
      601 atattggata ccccaaaaca gccaccaagt ggtttcaaaa tgaattttac cccagggtta
      661 acggtatttt ttttattcat cggaaggatg tttttaaatg gttagccttt cagaatattt
      721 ttgattactc tcctgttgaa accctcgaac attttgttag cacaactgat ggaaaacggc
      781 tagtgatttg cgatgagata atcctcgggg gactcgacat aggcttcggt tcgggcgagt
      841 acgtttgcat gatggccaac cggctgaagg aaacctttgg cgaggcctcc atcatcatct
      901 ttattcgcaa ccagcactct gcactcgaat cggtttatgg ccactacatc aaatcgggag
      961 gcacatactc agttaaaaaa tacatgggaa tttacagaaa gtttacaagg ccattccatg
     1021 attaccatct cttcaatccc gatttgttcc agtacaacaa aatagttgag ctgtatgccc
     1081 ggctatttgg gagtgagaat g
//
LOCUS       DAOOQX010000738         1100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_187685_length_1100_cov_2.343541, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000738 DAOOQX010000000
VERSION     DAOOQX010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187685_length_1100_cov_2.343541"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1100
                     /locus_tag="PLX53_09925"
     CDS             <1..>1100
                     /locus_tag="PLX53_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73442.1"
                     /translation="TIKSAWFYEIDSMDYLNDSILPNPISIKKTGNLYFAPDTIRIAG
                     KSRMGFGVEAFDIINGSSTTCGFHKLSMLVNNKQTFSFAVDSFSYAETRCLNSIIDYS
                     IRQLRQQEIVRLWADPGNLFSGLKADKSLGILTVESDSIYKITIELEDFYKNTSKLEM
                     VVKGVHPFKNPESTPGLLRHSYVSWWNSYSFDSKDYSIYIPRRTLYHDILLRHSALHT
                     DSLAFPVISIHHARTPLNGKFTLRVKIAGVPANLRDKCFIGYINGKGIEYCSSRQDGP
                     DMVTSPDKFGKFTLALDTVPPRIIPLNISQNADIAGQSSIKIKLTDNYGIGTYSGFID
                     DGWVLFEWDPKTSLLTHTLTPKRERQNTWHTL"
BASE COUNT          333 a          261 c          232 g          274 t
ORIGIN      
        1 acaatcaaaa gcgcatggtt ttacgagatt gacagtatgg actacctaaa cgatagcata
       61 ctgccaaacc ccatcagcat taagaaaaca ggaaacctgt actttgctcc cgacaccatc
      121 agaattgccg gaaaaagcag gatgggtttt ggagttgagg cattcgacat tatcaacggg
      181 agtagtacaa cctgcggctt ccacaagttg agcatgctgg taaataacaa acagaccttt
      241 tcctttgctg ttgactcgtt ttcgtatgca gaaacccgat gcctaaatag cattattgac
      301 tacagcatac ggcaacttcg acaacaggaa attgtacgcc tgtgggctga tcctggtaat
      361 ttgtttagcg gattaaaggc agacaaatca ttgggaattt taacggttga atccgacagc
      421 atttataaga taaccataga acttgaagat ttttataaaa atacttccaa actcgagatg
      481 gtagtaaagg gagttcatcc gttcaaaaac ccggaatcaa cccccggcct tttgcgacac
      541 agctatgtga gctggtggaa cagctatagc ttcgatagca aggattactc aatctacatt
      601 ccccgccgaa ccctttacca tgacattctc ctcaggcatt cagctcttca taccgacagc
      661 ctggcatttc ccgtaattag cattcaccat gcgaggactc cccttaacgg gaaatttacc
      721 ctaagggtaa aaattgcagg cgttccggcc aacttgcgtg ataaatgttt tattggatac
      781 attaatggta aaggtatcga gtactgctcc agccggcagg atggacccga catggttacc
      841 agccccgaca agtttgggaa gtttaccctg gctttggaca ccgttcctcc acgcataatt
      901 cctctaaata tttcacaaaa tgcggatatt gcgggtcaaa gcagcataaa aattaaactt
      961 accgataact acggaattgg cacctattca ggattcatag atgatgggtg ggttctgttt
     1021 gaatgggacc cgaagaccag cctgctaacc cataccctca cacctaaaag ggaaaggcaa
     1081 aacacctggc acacccttag
//
LOCUS       DAOOQX010000739         1099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_187882_length_1099_cov_3.086207, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000739 DAOOQX010000000
VERSION     DAOOQX010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187882_length_1099_cov_3.086207"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PLX53_09930"
     CDS             <1..886
                     /locus_tag="PLX53_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73443.1"
                     /translation="FAVQNYPITPGPTEKTIRQATDWIKQEGLNNVKFHYNDPTVVLQ
                     LNATPFDTTECERFVATPANPAKGLENGELLIYDTYFTEMAGGNLNELASCSDLELLK
                     VFEPEVPVDVYRKPYKIAVFKKIEPNPFVIQKNSSLLKGEGIIFCTIKNFDFDSTTLQ
                     TEAKHIKPMGKNGSSCILLKESRDFCLKQNFELDSLKFVHPFELQVSMSILPTEPEKK
                     LRYVVEATSNNKQIAYKSYDITFTEQNTSDEWVNREFRIAVPEIANQKNVRIKTYIWN
                     NRKGRYLIDDYTISYRIQ"
BASE COUNT          388 a          236 c          208 g          267 t
ORIGIN      
        1 ttttgccgtg caaaactacc caattacacc cgggcctacc gagaaaacaa ttcgccaggc
       61 taccgactgg ataaagcagg aaggactcaa caacgttaaa ttccattaca acgaccccac
      121 ggtagtactc cagctgaatg caaccccgtt cgatacgaca gaatgtgaaa ggtttgtcgc
      181 aacacctgcc aaccccgcta agggattaga aaatggcgaa ctgctaattt acgacactta
      241 cttcaccgaa atggctggag gcaacctcaa cgagcttgcc agctgtagcg atctcgagct
      301 gttaaaagtt tttgaacccg aagtacctgt tgatgtatat cgaaagccat acaaaattgc
      361 tgtattcaaa aaaatcgaac ccaacccctt tgttattcaa aaaaacagct cactattaaa
      421 gggtgaggga attatattct gcaccattaa aaattttgat ttcgactcaa ctacccttca
      481 aaccgaagcc aagcacatta aacctatggg aaagaacggc tcaagctgta tactactgaa
      541 ggaatcgagg gatttttgcc ttaagcaaaa ctttgaactc gactccctaa agtttgttca
      601 tccctttgaa ctgcaggttt ctatgagtat tcttcccacg gagccagaaa aaaagttgag
      661 gtatgttgta gaggctacca gcaacaacaa gcaaatagcc tacaaaagtt acgacataac
      721 ctttaccgaa caaaacactt ctgatgaatg ggttaacagg gaattccgca tagccgttcc
      781 tgaaatagct aaccagaaaa acgtcaggat aaaaacctac atttggaata ataggaaggg
      841 aagatacctg attgacgact acacaataag ctataggatt cagtagaaag gatttggcta
      901 tccttagaaa attttcgctc tcatcaaagt aaaggaacga attgataaat cctaaaaccg
      961 attaggtttg gagtaaaagc aaagtttttg gagtgcccaa aagattatac catggaaaat
     1021 cttgttcatg ctgccacttc caaaaaaatg ttaatataag ttagccttta gtaaacagta
     1081 gtagaaaaga aaattccaa
//
LOCUS       DAOOQX010000740         1098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_188238_length_1098_cov_2.498562, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000740 DAOOQX010000000
VERSION     DAOOQX010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188238_length_1098_cov_2.498562"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLX53_09935"
     CDS             <1..250
                     /locus_tag="PLX53_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HOY73444.1"
                     /translation="NLHGGKRAYVITGQYEKVMPMDILPVHLIKAILANDIERMEQLG
                     IYEVVEEDMALCEYVCTSKTDVQQILRKGIESLIKELS"
     gene            273..>1098
                     /locus_tag="PLX53_09940"
     CDS             273..>1098
                     /locus_tag="PLX53_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOY73445.1"
                     /translation="MGLRSYIDKIKPNFQKGAKYGMFHSTFDAFETFLFVPNRVTTKG
                     SHIRDSIDMKRTMTVVIVALLPALFFGMWNVGYQHALSIGESWPFWQTFFFGFLRVLP
                     LLVVSYGVGLSVEFAFAQARGHEVNEGFLVSGLLIPLILPVDVPLWMVAIATAFAVIV
                     GKEVFGGTGMNIFNPALLARAFLFFAYPSHMSGDKVWIDGLAKGQQVVDGFSGATPLA
                     NAAAYQLDKIPSAYDMFMGFIPGSIGETSKLAILIGAILLIITGIGSWKIIISVFGG"
BASE COUNT          253 a          235 c          277 g          333 t
ORIGIN      
        1 caatttacat ggtggaaaga gagcctacgt tataaccgga cagtacgaaa aggttatgcc
       61 aatggatatc cttcctgttc acctaattaa ggccatcctg gccaacgata ttgagcggat
      121 ggagcagctg ggtatttacg aggtggttga ggaggatatg gcactctgtg aatatgtttg
      181 cacctctaaa accgacgtgc agcagattct tcgaaagggc atcgagtcgt taattaagga
      241 gctaagctag acatttaatt atacctcaaa caatgggatt acgaagctac attgataaaa
      301 ttaaacctaa ctttcaaaag ggagcaaagt acgggatgtt ccattctacc tttgacgctt
      361 ttgaaacctt tctttttgtt cccaataggg taaccaccaa gggaagtcac ataagggact
      421 ctattgacat gaagcgtacc atgacggtgg tgatagtagc gctactgcct gccctctttt
      481 ttggtatgtg gaatgttggc taccagcacg ctctctccat tggagaaagc tggcctttct
      541 ggcagacctt cttttttggc tttttacgtg tgctacccct tttagtggta agctatggcg
      601 tggggctttc agttgaattt gcatttgccc aagctcgtgg acacgaggtc aacgagggat
      661 ttctggtttc cggtttgctt attcccttga ttttgcccgt tgatgttccc ctttggatgg
      721 ttgctattgc taccgccttt gcggtgattg ttggcaagga ggtgtttggc ggtaccggga
      781 tgaatatttt taatcctgca ctgcttgcca gggcattcct cttttttgcc tatccctcgc
      841 acatgtcggg cgataaggtt tggattgatg ggctagccaa agggcaacag gtagtggatg
      901 gtttttccgg ggctacgcca ttggctaatg ccgctgccta ccagctcgac aaaattcctt
      961 ctgcctatga tatgtttatg ggatttattc ccggttccat aggcgaaacc tccaagctgg
     1021 ccattctgat cggggcaatt ctacttatta tcaccggaat aggcagctgg aagattatca
     1081 tatcggtgtt tggtggcg
//
LOCUS       DAOOQX010000741         1097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_188456_length_1097_cov_2.838772, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000741 DAOOQX010000000
VERSION     DAOOQX010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188456_length_1097_cov_2.838772"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PLX53_09945"
     CDS             complement(<1..1089)
                     /locus_tag="PLX53_09945"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOY73446.1"
                     /translation="MNAILNAIVFGVSIIVIYTSLGLLVSLFNLGPDFTTTLTTHWIT
                     NLIFFLLFIAFAASFFGLFEIVLPGSVANKTDSKADKGGFAGSFFMALTTVIVSLSCV
                     GPIVGALLVEAASGLGAKPIVGMFGFSLAFSIPFTLFAIFPSWLNSLPRSGGWMNSVK
                     VVLGFIVLAFSLKFLLTIDQSYNLNFLSRDIYLAIWIAIFTLLGLYLLGKIKFKFDSD
                     LQHVGVLRLFLAIASFTFVVYLIPGMFGAPLKAISGLIPPQSSQKFSLNQNSQAVMPT
                     VNDNTNLSLCDQPKYADIFKLPYNLKGYFDYNQALECARKLGKPIFVDFVGHTCANCK
                     DMEARVWSDPRVLERLNSSYIIVALYVDD"
BASE COUNT          342 a          239 c          259 g          257 t
ORIGIN      
        1 atcatcaaca tagagggcaa caataatgta gctgctgttt aagcgctcca gcacccgagg
       61 gtcgctccac accctggctt ccatatcctt acagttagca caggtgtggc caacaaaatc
      121 aacaaaaatt ggcttgccca acttcctggc gcactccagc gcctgattgt aatcgaaata
      181 cccctttaag ttgtagggaa gcttaaaaat atctgcatac tttggttgat cgcaaagcga
      241 caagttggtg ttgtcgttta cagtgggcat cacagcctgg ctattctggt taagagagaa
      301 tttctggctg ctttgtggag gtataagacc ggaaattgct tttagaggtg caccaaacat
      361 gcccgggata aggtaaacta caaaggtaaa gctggcaata gccaaaaata accttaacac
      421 gccaacatgt tgaaggtcgg aatcgaactt gaattttatt tttcctagta ggtaaagtcc
      481 aagcagggtg aatatggcta tccatattgc caggtatatg tcacgactta aaaagttaag
      541 gttgtacgat tggtcgatgg tgagtagaaa ctttaaggaa aaagccagca caatgaaacc
      601 caacaccacc ttcacggagt tcatccatcc acccgagcgg ggtagcgaat ttagccacga
      661 tgggaaaatg gcaaatagtg taaagggaat tgagaaggcc aacgaaaatc caaacattcc
      721 aacaataggt ttagcaccta gcccagaggc tgcctcaaca agaagcgctc ctacaattgg
      781 gccaacacat gacaggctga ctataaccgt tgtgagtgcc atgaaaaacg agcccgcaaa
      841 tccaccctta tcggccttac tatcggtttt gttggctacg cttccgggta gaacaatttc
      901 aaatagccca aagaaggatg ccgcaaatgc aatgaaaagt agaaagaata tcaggttggt
      961 aatccagtga gtggtgagcg tggtggtgaa gtcgggtccc aggttgaaca gcgataccag
     1021 taaacccaac gaggtgtata tcacaatgat ggacacgccg aaaactatgg cgtttagtat
     1081 agcattcatt ctgttgc
//
LOCUS       DAOOQX010000742         1096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_188816_length_1096_cov_2.554275, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000742 DAOOQX010000000
VERSION     DAOOQX010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188816_length_1096_cov_2.554275"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..790
                     /locus_tag="PLX53_09950"
     CDS             281..790
                     /locus_tag="PLX53_09950"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HOY73447.1"
                     /translation="MKQLAILLVTLVFPLVLEAQGIAEEIFERYAGKPGYTSVAIGSE
                     FLNFVAALDREDNELQDLSNKISSLKILVAKQQVEEFGKTLERGITREGYKEVMQAND
                     EGGLVTFYALPKGDKFSNFILHVTNSDEEVMLAIAGNFTLSDLSKLGGSSEDDDSYIS
                     LLRKLENEK"
     gene            complement(845..>1096)
                     /locus_tag="PLX53_09955"
     CDS             complement(845..>1096)
                     /locus_tag="PLX53_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOY73448.1"
                     /translation="EIPVNIYDLGLIYEVDVDSNSKVTITMTLTAPNCPLADDVVDEV
                     HEKVSAIEGVSGVVVNLTFDPPWDKSRMSDEAMLELGFL"
BASE COUNT          338 a          228 c          249 g          281 t
ORIGIN      
        1 aaacgtaggc gcgattttgg cttttcagtg tcatccgaag gtagttcaaa taattgggca
       61 agttagaaat aatcgcttag ccggagaagg aaatgccgaa atactacccc tcttaaccaa
      121 aaaataaaga attcgccatg cagcaagttt ggctttacta tcgtatttaa tagttgaaat
      181 taaaagcaaa atgaaccatc cttaaaaatg gcagctagca tatacacatt gctagtggcg
      241 gatggtttgg taacttatca aaacaactaa aaatatgcac atgaaacagt tagcaatttt
      301 actggttact cttgttttcc ctttagtgct ggaagcacag gggattgccg aagaaatatt
      361 tgagcgctat gccggaaagc ccgggtacac ctccgtcgcc attggttctg aatttttaaa
      421 ctttgtggca gccctagaca gggaggataa tgaactgcag gatttatcga ataaaatcag
      481 cagcctgaaa attttagtgg ccaagcagca ggttgaagaa ttcggcaaaa cgctggaaag
      541 gggaattacc cgcgaagggt ataaggaggt tatgcaagcc aacgatgagg gtgggttggt
      601 aacattctac gcactcccaa aaggagacaa gttttccaac ttcatactcc acgtaaccaa
      661 ttcggatgag gaggtaatgc tggccattgc gggaaacttt acgctatcgg atctgtcgaa
      721 gcttggtggc tcctcagaag atgatgatag ctacatttcc ctgttaagaa agctggagaa
      781 cgaaaagtaa gctcaaaacg aacatccttc gacaaggttg atgtgtagct gatttcttct
      841 aattttacag aaatcccagc tccaacatgg cctcgtcgct catccggctt ttatcccagg
      901 gcggatcaaa ggtaaggttg accacaaccc ccgaaacccc ctcgatagca gatactttct
      961 catgcacctc atcaaccaca tcatcggcaa gcgggcagtt gggtgcagta agggtcatgg
     1021 taatggttac tttagaattt gagtcaacat caacttcgta gattagccct aagtcataaa
     1081 tatttacagg gatttc
//
LOCUS       DAOOQX010000743         1096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_188819_length_1096_cov_2.533141, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000743 DAOOQX010000000
VERSION     DAOOQX010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188819_length_1096_cov_2.533141"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PLX53_09960"
     CDS             <1..755
                     /locus_tag="PLX53_09960"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOY73449.1"
                     /translation="NLKRIDFTVEWHNSVTSSNSICAQRCEEGAAEGLVIAAMFQEMG
                     RGQRGNSWESEAGRNLTFSILLRPGHLRVDEQFYLSKVAAASVCDWVSAYVSGDDVTI
                     KWPNDIYIGDSKVAGILIENSFSSAMLDTSVVGIGINLNQQTFPDDIPNPTSLRLATG
                     MEFDLHSSLAEFLECFARRYELLSDEQSRFQLDEYYLKKLYRRDLYHGYCDKNGNFKA
                     KIAGVKPTGELVLQLPTGELRVYAFKEVMFLI"
     gene            863..>1096
                     /locus_tag="PLX53_09965"
     CDS             863..>1096
                     /locus_tag="PLX53_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOY73450.1"
                     /translation="MKRSVFAACFIVTALFSQLLFGQEPQKESESYVFTPIKELKVTP
                     VKNQNRSSTCRSFSGVAFLESELLRMGKPEVNLS"
BASE COUNT          283 a          230 c          276 g          307 t
ORIGIN      
        1 aaaacttgaa aaggattgat tttacagttg agtggcataa cagcgtaacc tcttccaata
       61 gtatttgtgc tcaacgttgc gaggagggtg ctgctgaagg gctggttatt gccgccatgt
      121 ttcaggaaat gggccgaggg caacgcggca actcgtggga gagcgaagct ggcagaaacc
      181 tgacatttag catactgctt aggccagggc atctcagggt tgatgagcaa ttctacctgt
      241 caaaagttgc tgctgcttcc gtgtgcgact gggtgagtgc ttacgtttcg ggcgatgacg
      301 ttaccattaa gtggcctaac gacatctaca ttggcgattc aaaggtagcc ggcattctga
      361 tcgagaacag ctttagctcg gcaatgctag acacatcggt ggttggcatt ggaataaacc
      421 tgaaccagca aaccttcccg gacgatattc ccaacccaac ctcgctaagg ttagctaccg
      481 ggatggagtt cgatctgcac tcctcgctcg cagaatttct ggaatgtttt gcccggcggt
      541 acgaattgct ttccgatgag cagagccgtt tccagctgga cgaatactac ttaaagaagc
      601 tttaccggcg agatttgtac catgggtact gcgacaaaaa tggtaacttt aaggctaaaa
      661 ttgctggtgt aaagcctacg ggggagctgg tcttgcagct tcctacgggt gagttgaggg
      721 tatatgcctt taaggaggtt atgtttctta tttaaacgtg ttagtaattt actgtctggc
      781 tacttttaaa ctttttttta aatccggaat ctattaaact agctaagcaa tgtttaacat
      841 gaacattaaa ctgtatgaaa ctatgaaaag atctgtattt gcagcgtgtt ttattgtaac
      901 ggcccttttt agccagctgc tttttggcca ggagccacaa aaggaatcgg aaagttacgt
      961 ttttacccca attaaggagc ttaaggttac accggttaag aaccagaatc gctcaagtac
     1021 ctgccggagc ttttcaggtg ttgcctttct ggaatcggag ctacttcgca tgggtaaacc
     1081 cgaagttaac ctttcg
//
LOCUS       DAOOQX010000744         1094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_189360_length_1094_cov_3.358999, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000744 DAOOQX010000000
VERSION     DAOOQX010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189360_length_1094_cov_3.358999"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLX53_09970"
     CDS             complement(<1..563)
                     /locus_tag="PLX53_09970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOY73451.1"
                     /translation="MSIHHEVAVVILNWNGEKFLQRFLPGVLANSNETGVAVYVADNG
                     STDGSIKILRSRFPLVNLILLDRNYGYAGGYNRALSQIDSDFFVLLNSDVEVPGGWLN
                     PLRSFMRSNPNAAACAPAIMDIARPECFEYAGAAGGFIDKFGYPFCRGRILSEIEQDT
                     GQYNQSIEVFWASGACMMVRANTFWDAG"
     gene            complement(547..>1094)
                     /locus_tag="PLX53_09975"
     CDS             complement(547..>1094)
                     /locus_tag="PLX53_09975"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOY73452.1"
                     /translation="ELYGLFPLYIKHTVLGAYKPLNNKPIDEFIKKSRERFGCVTIPM
                     YSISRVALKWSQEGKPFFLGLINDQSPSRSHIGYWTTFLNQETPVYIGTEKIARKINQ
                     PVVFCYIRKLKRGKYEANVELLVEEPRLTKPHEITEMHLRALERVINEAPEYWLWSHK
                     RWKHKRDVTTNSLQAGNEHTP"
BASE COUNT          281 a          287 c          242 g          284 t
ORIGIN      
        1 cccgcatccc aaaaagtgtt tgccctcacc atcatgcagg ccccgcttgc ccagaatacc
       61 tcgatggact ggttgtactg cccggtgtcc tgctctattt cggatagaat cctaccccta
      121 caaaacgggt agccaaactt gtcaataaaa ccacccgccg ctccggcata ctcaaagcac
      181 tccgggcggg caatgtccat aatggcagga gcgcaagcgg ctgcatttgg gtttgaccgc
      241 ataaagctac gtaaaggatt aagccatccc ccaggcactt caacatcaga attaagcagc
      301 acaaaaaaat cgctgtctat ctggcttagc gcacggttat accctccagc gtagccataa
      361 ttcctgtcga gtaggatgag gtttaccagc ggaaaccttg atctgagaat cttaattgag
      421 ccatctgtag aaccattatc ggcaacataa accgccacgc cagtttcgtt ggagttggct
      481 agtactcccg gaagaaagcg ttgcaggaac ttctcgccat tccagttcag gataactacg
      541 gcaacctcat ggtgtatgct catttcctgc ctgtaaggag ttagtggtta catcacgttt
      601 atgcttccat cgcttatgag accagagcca gtactcagga gcttcattaa tcaccctttc
      661 caacgctcgg aggtgcatct cggtaatctc gtggggttta gtcagcctag gttcttcgac
      721 cagaagctcc acgttcgctt catacttgcc tcgctttaac ttgcgaatgt aacaaaacac
      781 cacgggttgg tttattttcc gggctatctt ttcggtccca atataaactg gagtctcctg
      841 gttcaaaaaa gtagtccagt acccaatatg gcttcgagat ggcgattggt cgtttataag
      901 ccccaaaaag aagggtttac cttcctgcga ccattttagg gcaactctcg aaatgctgta
      961 cattggtatg gtaacacacc caaaacgttc acgtgacttt ttgatgaatt catcaatcgg
     1021 cttgttatta agtggcttgt acgcacccag aacagtgtgt tttatgtaga gcggaaacag
     1081 gccgtaaagc tccc
//
LOCUS       DAOOQX010000745         1093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_189649_length_1093_cov_3.186898, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000745 DAOOQX010000000
VERSION     DAOOQX010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189649_length_1093_cov_3.186898"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1093)
                     /locus_tag="PLX53_09980"
     CDS             complement(<1..>1093)
                     /locus_tag="PLX53_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOY73453.1"
                     /translation="GKTMLLATVVSAQEAKEDADFMPLSVDYKEKFAASGRFPGGFFK
                     REAKPSDYEVLIARLVDRALRPLFPDDYHAETFVTINLISAEKEIMPDALAGLAASAA
                     LAVSDVPFHGPISEVRVARVNGKLLINPSFQQLEEADIDMIVGATYDNILMVEGELKE
                     VSEDEMLEAIKFAHEAIKPQCLAQMELAKEIGVVKRTYCHEVNDEQLKASVKEFCYDK
                     VYSIAKSMLPKQERTAAFEAIKQSFMETIPEEERESKEVMVNRYYHAVEKEAMRNMIL
                     NEGVRLDGRKTNEIRPIWCEVDYLPMAHGSAIFTRGETQSLTTVTLGTKLDEKIIDEV
                     LLQGTEKFTLHYNFPPFSTGEAKASRGVSR"
BASE COUNT          246 a          276 c          265 g          306 t
ORIGIN      
        1 gacggctaac ccctctggag gcttttgcct caccggtgga aaagggtgga aagttgtagt
       61 gaagagtaaa tttttccgta ccctgaagca gcacctcgtc tattatcttc tcgtcaagct
      121 ttgttcctaa cgtaactgtg gttaacgact gggtttcacc acgggtgaat atagccgacc
      181 cgtgagccat gggaaggtag tcaacttcgc accagatggg tcgaatttcg ttggtttttc
      241 gaccatcaag gcgaacgccc tcgttgagta tcatgttgcg catggcctcc ttttcaactg
      301 catggtagta gcggttaacc attacctcct tcgattcccg ttcctcttcc ggaatagttt
      361 ccatgaacga ctgcttgatg gcttcaaagg cagctgttcg ctcctgcttt ggcagcatgg
      421 atttggcaat gctgtaaact ttatcgtagc aaaactcttt tacactagct tttagctgct
      481 catcgtttac ctcgtggcaa tacgtacgtt taaccacgcc tatctccttg gccagctcca
      541 tttgagccaa gcactggggt ttgatggctt catgggcaaa ctttattgcc tcgagcattt
      601 catcctccga aacctccttt agctcccctt caaccatgag gatgttgtcg taggttgccc
      661 ctacaatcat gtcgatgtct gcctcctcaa gctgttgaaa ggatgggttg attagtagct
      721 tgccgttcac cctggccacg cgaacttcgg atataggacc atgaaatgga acatccgata
      781 cggcaagagc agcggatgct gccaagccgg cgagtgcgtc gggcataatt tccttttcgg
      841 ccgaaattag gtttatggta acgaaggttt cggcatggta gtcgtcaggg aataacggcc
      901 gaagggcacg gtcaaccaga cgggcaataa gcacctcgta atcggaaggt tttgcctcac
      961 gtttaaagaa accacctgga aaacgtccgc tggctgcaaa tttttccttg tagtccacgc
     1021 ttagcggcat gaaatctgca tcttcttttg cttcctgagc gcttacaact gtagcgagaa
     1081 gcatggtttt acc
//
LOCUS       DAOOQX010000746         1093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_189659_length_1093_cov_2.983622, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000746 DAOOQX010000000
VERSION     DAOOQX010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189659_length_1093_cov_2.983622"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1093)
                     /locus_tag="PLX53_09985"
     CDS             complement(<1..>1093)
                     /locus_tag="PLX53_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HOY73454.1"
                     /translation="GHASTSISAALGMAVASELKHESHQVVAIIGDGALTGGLAFEGL
                     NNGGSLKSNLLVILNDNHISIDPGVGALNEYLLDITTSKTYNRIKKDVWDLLGRFDSL
                     GPKARSAIQKLDNAIKTALLRQSNLFESLGFRYFGPVDGHDVAYLIRILNDLKDIPGP
                     KLLHCITVKGKGYTPAEQNQTIFHAPGKFNKDTGERISLLTSKPAPPRYQDVFGETLV
                     ELAQSNDRIVGITPAMATGCSMNIMMEKMPERTFDVGIAEQHAVTFAAGMAADGLIPF
                     CNIYSSFMQRAFDQVIHDVALQNLHVVFCLDRAGLVGDDGPTHHGAFDLAYLRLIPNI
                     TIAAPMNEEELRNMMFTAQLDGQGTFSIRY"
BASE COUNT          266 a          258 c          279 g          290 t
ORIGIN      
        1 gtagcgtatg gagaatgtac cctgcccatc aagttgggca gtaaacatca tgtttcgcaa
       61 ctcctcctcg ttcataggag ccgcaatagt gatattgggg attaacctta agtaggctaa
      121 gtcgaaagct ccgtggtggg ttggcccatc atcaccaacg agtccggcac ggtcaaggca
      181 aaacaccacg tggagatttt gaagggctac atcatgtatt acctgatcga atgccctctg
      241 catgaagctg gagtaaatgt tgcagaacgg tataaggccg tcggcagcca taccggcagc
      301 aaatgtaaca gcgtgttgct cggctatgcc cacatcaaat gttctttctg gcattttctc
      361 catcataatg ttcatggagc atccggtggc catagcagga gtaattccaa ctattcggtc
      421 gttactttgg gcgagttcaa ccagggtttc accaaaaaca tcctgatacc ttggtggggc
      481 tggcttgctg gtaaggaggc tgattcgctc accggtgtcc ttgttgaatt ttccgggagc
      541 gtggaagatt gtttggtttt gctcggcagg agtatagcct tttcctttaa cggttatgca
      601 gtgcaggagt ttaggacccg gaatatcctt caaatcgttt aaaattcgta tcaggtaggc
      661 cacatcgtgc ccgtcaacag ggccaaagta gcgaaatccc aaagattcaa acaggttact
      721 ttgcctgagc agggctgttt tgatagcatt gtcgagtttc tggatagccg aacgtgcctt
      781 ggggcctaag ctgtcaaacc ttcccagtaa atcccaaaca tccttcttta tcctgttgta
      841 ggttttggag gtggttatgt cgaggaggta ctcgtttagc gccccaaccc cggggtcaat
      901 agatatatgg ttatcgttaa gaataaccag caggttcgac ttcaagctac cgccgttgtt
      961 aagcccttca aaggccaatc cgccggttag cgctccatca ccaataatgg caacaacctg
     1021 gtgtgattcg tgttttaact ctgaggcaac cgccattccc agtgcagcag aaatggaggt
     1081 tgaggcgtgc cca
//
LOCUS       DAOOQX010000747         1090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_190548_length_1090_cov_2.850242, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000747 DAOOQX010000000
VERSION     DAOOQX010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190548_length_1090_cov_2.850242"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..>1090)
                     /gene="nqrF"
                     /locus_tag="PLX53_09990"
     CDS             complement(5..>1090)
                     /gene="nqrF"
                     /locus_tag="PLX53_09990"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOY73455.1"
                     /translation="SILSTLSNNKIFLPSACGGGGTCGLCRCRVVEGGGSMLPTEANF
                     FNRRQQREHWRLGCQVKVREDLSIIVPEEVLGIKKWECEVISNRNVATYIKEFVVKLP
                     DGEKLNFRSGGYIQIDVPKIEVDFSKDIFVEPEFHDDWDKTNMWSLKMKNTEETFRAY
                     SMANHPAENNIIMLNVRIATPPWDRERNTFLKVNPGVCSSYIFSRKPGDKVTISGPYG
                     DFFIKDTQNEIMFIGGGAGMAPMRSHIFHLFNTLKTNRKVTFWYGARSRREIFYEEHF
                     RKIAQEFPNFNFTIALSEPKPEDSWDGPVGFIHQVIFEQYLSRHNAPEDIEYYLCGPP
                     AMIAAVQKMLYNLGVPDEMIAFDDFGG"
BASE COUNT          262 a          281 c          265 g          282 t
ORIGIN      
        1 ctggttatcc tccaaaatca tcaaaagcaa tcatctcgtc ggggactcca aggttgtaaa
       61 gcattttctg aacggcagca atcatggccg ggggtccgca aaggtagtac tcaatgtcct
      121 cgggggcatt gtgcctggag aggtattgct caaatataac ctgatggata aaccccacag
      181 ggccatccca gctatcctcg ggtttgggct cggacagggc aatggtaaag ttaaagttgg
      241 ggaactcctg ggcaatcttc cggaaatgct cctcgtagaa tatttcgcgc ctggagcggg
      301 ctccgtacca aaaggtaacc ttacggttgg ttttgagcgt gttgaacaga tgaaagatat
      361 gcgaacgcat gggggccatt ccggctcctc ccccaataaa cataatttcg ttctgtgtgt
      421 ctttaataaa aaaatcacca tacggcccgg aaatggtgac cttatcgcca ggctttcggg
      481 aaaatatgta gctggagcaa acgccggggt taacctttaa aaaggtattc cgctccctgt
      541 cccatggtgg agtggcaatg cgcacgttca acataatgat gttgttctct gccggatggt
      601 tagccatgga gtatgccctg aaggtttcct cggtattttt cattttcagg ctccacatat
      661 tcgtcttgtc ccagtcgtcg tgaaactcgg gctccacgaa tatatcctta gaaaaatcta
      721 cctcgatttt aggaacatcg atttgaatgt agccgccgga gcggaagtta agtttctcac
      781 catcgggtag cttcaccacg aactccttga tgtatgtggc cacattccgg ttagaaatca
      841 cctcgcactc ccacttttta attccaagta cttcctctgg cactatgatg ctcaggtctt
      901 cgcgaacctt aacctgacaa cccaggcgcc agtgttcccg ctgctgccta cggttgaaaa
      961 agtttgcctc ggttggcagc attgagcctc cgccctccac cactctgcac ctgcacaacc
     1021 cgcaggttcc gcctcctccg cacgccgagg ggagaaaaat tttgttgttg gacagggttg
     1081 aaagtatgga
//
LOCUS       DAOOQX010000748         1088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_191145_length_1088_cov_3.135528, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000748 DAOOQX010000000
VERSION     DAOOQX010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191145_length_1088_cov_3.135528"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLX53_09995"
     CDS             <1..638
                     /locus_tag="PLX53_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73456.1"
                     /translation="VRPIYFGTTVNSDTYHGRNKYFHLEGLMYRIFPVEAGSQWGVGS
                     VNTSEMYGNLMGKYRYRGLNNPKVYIDENKFRIVSNYRNLYARLANALVDEGKTDSAL
                     VVLNHCMEVIPASTVPLNYYVLPIIEGYYRAGDKTSAIRISRLLMDQSVKELDFILTQ
                     LNDRQQQNLFNSVQLDMAILQDLVRMAQSYEKGNHYEELNRTFNRFLSMLQ"
     gene            645..>1088
                     /locus_tag="PLX53_10000"
     CDS             645..>1088
                     /locus_tag="PLX53_10000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOY73457.1"
                     /translation="MINLSPPRFFQRFFKNIIWNFPDEKEGVFLTFDDGPTQGVTPWV
                     LDQLARYNAKATFFCLAKNVEMNPDIFERVVREGHAIGNHSYSHIKGWRTDTTQYIQD
                     VIYANSFLHTNLYRPPYGRITARQFRLITYQYKIVLWDVLSMDYSR"
BASE COUNT          299 a          237 c          262 g          290 t
ORIGIN      
        1 gggtgcgccc aatttacttt ggaactacgg tgaatagcga tacataccac gggcgtaata
       61 agtattttca ccttgaagga ttgatgtaca ggattttccc tgttgaggca ggatcacagt
      121 ggggtgtggg cagcgtaaac acctccgaaa tgtacggcaa cctgatgggt aagtacaggt
      181 atagagggct gaacaacccg aaagtttaca ttgatgagaa caaattccga attgtctcca
      241 actataggaa tctttatgcc cgcctggcca acgcattggt ggatgagggc aaaaccgact
      301 cggctttggt tgtgctaaat cattgcatgg aggttattcc ggcaagcacg gtgccgttga
      361 actactacgt gctgccaatt atcgagggtt attacagggc aggagataaa acttcggcca
      421 ttagaatatc caggttgcta atggaccaat cggtaaaaga gcttgatttc atacttaccc
      481 agcttaatga taggcagcaa cagaacctct tcaacagtgt gcagctggat atggccatac
      541 tgcaggactt ggttcgcatg gcccagagtt acgagaaggg taatcattac gaagagctga
      601 atagaacatt taaccgtttt ctgtcgatgt tacagtaaat caacatgatt aaccttagtc
      661 ccccccgttt ctttcagcgt ttttttaaaa atattatttg gaattttcct gatgaaaagg
      721 agggggtctt tttaacattt gatgatgggc ctactcaagg ggttaccccc tgggtactcg
      781 accagcttgc ccgctacaac gctaaggcta catttttttg tctggctaaa aacgtggaga
      841 tgaatcccga tatctttgaa cgagttgttc gtgaggggca tgctattggg aaccactcct
      901 atagccatat aaagggttgg agaaccgata caacccagta cattcaggat gtcatttacg
      961 ccaacagctt cctgcatacc aacctctacc ggccgcctta cggcagaatt actgcacggc
     1021 agtttagact tatcacctat cagtacaaaa tagtgctttg ggatgtgctc agcatggact
     1081 acagccgg
//
LOCUS       DAOOQX010000749         1086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_191743_length_1086_cov_3.455868, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000749 DAOOQX010000000
VERSION     DAOOQX010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191743_length_1086_cov_3.455868"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="PLX53_10005"
     CDS             <1..1074
                     /locus_tag="PLX53_10005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOY73458.1"
                     /translation="NNNILIMANMKPTILGLTPLAIALVLTGCKTSKTEVPATEENVP
                     LVSTVVVEERIYSPTLSFTGTAFANREANLGATLPGKVEKIYFDEGQNVHEGDLIVEL
                     SDELLTQTIIENETIKKDFDRVSRLREKGSISEMEYDHVKAQYDASQAKVQMVKKNTR
                     VYAPFSGMVVERMMEEGEVYFLNPGLEPGYSMRSGIVRLMQLNPVKVRFDVNEKDLGV
                     IKKGLEVEVTFDGVAGKTYQGKVGTIKQILSTTTHSAAVEVTISNPQLEIKPGMFAYI
                     TVKLPEIKASVVPLKSIYRLPGTSDDYLFSVTNDTINRIPISRLESIGEYVAVKGIEP
                     GVEVVLDGKTRVSQGQRVRVSGK"
BASE COUNT          327 a          240 c          272 g          247 t
ORIGIN      
        1 aataacaata ttttaataat ggcaaatatg aaaccgacta tcctagggct aaccccactt
       61 gctattgcac tagtgctaac agggtgcaag accagtaaaa cagaagttcc cgcaacggag
      121 gaaaacgttc cgttggtttc gactgttgtg gttgaggagc gaatttatag ccccaccctt
      181 tcctttaccg gaacagcttt tgccaatcga gaggccaacc tgggggcaac tcttcccgga
      241 aaggttgaaa aaatttactt cgacgaaggg caaaacgtgc acgaaggcga cctgattgtt
      301 gagctatcag acgaactgct cacccagaca atcattgaaa acgaaaccat aaaaaaggat
      361 ttcgatcgag tttcgcgcct cagggaaaaa ggaagcatca gcgaaatgga gtatgaccac
      421 gttaaggctc agtatgatgc ctcacaggcc aaagtacaaa tggtcaaaaa aaacacaagg
      481 gtttatgctc ccttcagcgg tatggttgtt gagcgcatga tggaggaggg tgaggtttac
      541 ttcctaaacc ctgggctaga gccgggctac tccatgcgct cgggaattgt taggctcatg
      601 cagcttaacc ccgttaaggt taggttcgat gtcaacgaaa aggatttagg tgtcataaaa
      661 aaaggacttg aagttgaggt taccttcgat ggcgttgcag gcaaaaccta ccagggaaag
      721 gtgggaacca taaaacaaat tctgtcaacc accacccact cagctgcagt ggaggttacc
      781 attagtaacc cgcagctgga gattaaaccc ggaatgtttg cctacatcac cgtgaagttg
      841 cccgaaatta aggcttctgt agtgccatta aaaagcattt accgcctacc cggcacttcg
      901 gatgattacc ttttctcggt aaccaacgac accattaacc gaataccgat atccaggctt
      961 gaatcaatag gcgaatatgt tgcggtgaaa gggattgaac ccggcgttga agtggttctt
     1021 gatgggaaaa ccagggtaag tcagggacaa cgtgtacggg tcagcggaaa ataggctcgt
     1081 ttaggg
//
LOCUS       DAOOQX010000750         1084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_192368_length_1084_cov_2.803693, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000750 DAOOQX010000000
VERSION     DAOOQX010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192368_length_1084_cov_2.803693"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..647)
                     /gene="ruvA"
                     /locus_tag="PLX53_10010"
     CDS             complement(63..647)
                     /gene="ruvA"
                     /locus_tag="PLX53_10010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOY73459.1"
                     /translation="MIAFVRGQFAVKTPARVVVDVNGVGYDLQISLNTFSAISNKENG
                     QLFTYLHITENSHTLFGFAELSEKDLFLQLISVSGVGASTARMMLSGMKPEEITRAIV
                     QGNTKQLEGIKGIGKKSAERLIVELRDKLAKQHNENILPGFVTPQIDSDAINALISLG
                     IGRVMAEQAVKKTMQTSPENSSLEEIIKLALKNL"
     gene            719..>1084
                     /locus_tag="PLX53_10015"
     CDS             719..>1084
                     /locus_tag="PLX53_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HOY73460.1"
                     /translation="MNKRSAAITAVGGYVPETKLTNFDLEKMVDTNDEWIRTRTGIEE
                     RRILKEPGKASSDMAVPAIQEILRKKNLAPTDIDCIICATVTPDMVFPATANIIADKL
                     GATNAWGFDMSAACSGFLYA"
BASE COUNT          338 a          214 c          219 g          313 t
ORIGIN      
        1 acaaatgagt aggaatactt caacaacagt cccgcttggt taagtaaagc tcaattcgat
       61 ggctataggt ttttgagtgc tagtttaata atttcttcta atgaactgtt ttcgggggat
      121 gtttgcatgg ttttcttaac agcttgttcg gccattactc taccaatgcc caatgaaatt
      181 aaggcattta ttgcatccga atctatttgc ggggtaacaa atccgggtaa aatattttca
      241 ttgtgctgtt tggccagctt atcccttagt tcaacaatca atctttcggc cgatttttta
      301 ccaattccct tgattccttc taattgtttg gtgttaccct gaacaatggc tcgggtgatt
      361 tcctcgggct tcatgccaga caacatcata cgagcggtgg atgctcctac tccagacaca
      421 ctaatcaact gtaaaaaaag gtctttctca gacaattcag caaagccaaa aagggtatgt
      481 gaattctcgg tgatatgcaa ataagtaaac aattgcccgt tttctttatt gctgatggca
      541 gaaaaggtat tgaggctaat ttgaagatca taccctactc cattcacatc taccactaca
      601 cgggcagggg ttttaacggc aaattgacca cgaacaaaag ctatcataat tgagacgaaa
      661 atagcaaaaa gaaagcaatt agccgtacct tcaatctttt atataacctt gataataaat
      721 gaataaaaga agcgcagcta ttaccgcagt gggaggatat gttcccgaaa caaagttgac
      781 caatttcgat ttagaaaaaa tggtggatac caacgatgag tggatcagaa caagaacagg
      841 catagaagaa agaaggattt taaaagagcc aggcaaagcc agtagcgata tggcagttcc
      901 agccattcaa gaaattcttc gtaagaaaaa tcttgctcca accgatattg attgcattat
      961 ttgtgcaact gtaacccctg atatggtttt tccagcaacc gctaatatca tagccgataa
     1021 gctaggtgca accaatgctt ggggatttga tatgagtgcg gcctgtagcg gttttttata
     1081 tgcc
//
LOCUS       DAOOQX010000751         1082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_192965_length_1082_cov_2.979552, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000751 DAOOQX010000000
VERSION     DAOOQX010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192965_length_1082_cov_2.979552"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PLX53_10020"
     CDS             <1..875
                     /locus_tag="PLX53_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOY73461.1"
                     /translation="RYRDNLPGKGNGVEQVFRGLGLDDSIFRASHSIGLGAQFGGKYL
                     ALDVRIIRLPRHGASCPVGIGVSCVADRNVKAKINRHGIWVEKLENNPGRFIPEQWRK
                     PDEEGAVGIDLNQPMETIRAELSKHPVGTRLSLSGKIVVARDIAHARLKERMDRGEGL
                     PQYLKNHPVYYAGPAKTPEGMPSGSFGPTTAGRMDSYVDAFQSQGASLVMIAKGNRSA
                     AVTEACKKYGGFYLGSIGGPAAILAHENIKKVELLEYPELGMEAVYMIEVSDFPAFIL
                     VDDKGNDFYKQVAR"
BASE COUNT          256 a          239 c          312 g          275 t
ORIGIN      
        1 ctaggtatcg cgacaacctg cccggtaagg ggaatggggt ggagcaagtt tttaggggtt
       61 tgggtcttga tgatagtatt ttccgtgcat cccacagtat aggacttggt gcacagttcg
      121 ggggcaagta ccttgccctg gatgttagga ttatcaggct tccccgccat ggcgcatcct
      181 gtccggtagg aatcggggta tcatgcgtgg ccgataggaa tgtaaaggcc aagataaacc
      241 gccacggtat ctgggttgaa aagttggaaa acaacccggg aaggtttatc cccgagcagt
      301 ggcgtaaacc tgatgaggaa ggcgctgttg gtatagacct gaatcagcca atggagacta
      361 ttcgagccga gctatcgaag cacccggttg gcacacgact ttcgttaagc ggcaaaattg
      421 ttgtggcccg cgatattgcc catgcccgac ttaaggagcg tatggatagg ggggaggggc
      481 tgcctcagta ccttaagaat catccggttt actacgccgg tcccgctaaa accccagagg
      541 gtatgccatc gggctctttc ggccccacca ctgccggccg gatggactcc tatgttgatg
      601 cattccagtc gcagggagca agcctcgtga tgattgccaa gggtaacagg agcgccgcag
      661 ttactgaggc ctgcaagaaa tatggaggct tttacctggg aagcataggc ggacctgctg
      721 ccattcttgc gcacgagaat attaaaaagg tggagctgct ggagtacccc gagcttggaa
      781 tggaggcagt ttacatgatt gaggtttccg actttcctgc tttcattctg gttgatgaca
      841 agggcaacga tttttacaag caggtggcca ggtgatttcg atggcgcttg gtggatttct
      901 gctaccattc ggccaaatat cagcatggtg tttaaggaaa tttggagggg aaatttcgtc
      961 actcatgaat gcggcttacc gtcttgctat aagcagtatc cgcgtggcta tcaatttagt
     1021 aatagaattc aaatttatgg aaatgagaaa gcttggtttt atcctgctat tcttttgtct
     1081 cc
//
LOCUS       DAOOQX010000752         1082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_193026_length_1082_cov_2.434275, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000752 DAOOQX010000000
VERSION     DAOOQX010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193026_length_1082_cov_2.434275"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PLX53_10025"
     CDS             complement(<1..912)
                     /locus_tag="PLX53_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HOY73462.1"
                     /translation="MASNFLRRVDLIFDQDCNFKEVLKSAYQHSNDEIINQLLESGLK
                     GRGGAGFPTALKWKFTSEQADPEKYVVCNADEGEPGTFKDREILFKVPLKVFGGMAIC
                     AKVINAKEGFIYLRGEYRFLLSELENQLKLFNEILKDLKIDFKIKIIVGSGAYICGEE
                     SALFESMEGDRGEPRNKPPYPTVSGFRKKPTVINNVETLVYAFMIFRYGARKFKELGT
                     ADSRGSKVFSVSGDTPKPGIYELELGMTVEQFVDEFGDGDTKAVQVGGASGFCVPRKK
                     FKDTIIGFEGVPTGGSMMLFNSSRSMYN"
BASE COUNT          281 a          279 c          218 g          304 t
ORIGIN      
        1 gttgtacatg gatcgcgagc tgttaaagag catcatggaa cctccagtag gaacgccttc
       61 aaatccgatt atggtatctt tgaacttttt tcggggaacg caaaaccctg aagcgccacc
      121 cacctgaacc gctttggtat caccatctcc aaactcgtca acaaattgct cgacggtcat
      181 acccagctcc aactcataaa ttccaggttt tggagtatct ccagatacag aaaacacctt
      241 agacccgcgt gaatctgcag ttcccagttc cttgaatttg cgggcgccat agcggaaaat
      301 cataaaggca taaaccagcg tttcaacgtt gttgataacg gtaggttttt tccggaatcc
      361 cgatacggta gggtatggag gtttgttgcg aggttcgccc ctgtcgcctt ccatcgattc
      421 gaaaagagcg ctttcctcac cgcatatata ggcgccacta cccactataa tttttatttt
      481 aaaatcaatt ttcaaatctt tcaaaatctc gttgaacagc tttagctgat tttccagctc
      541 cgaaagtaaa aagcgatact ctcctctaag gtagataaat ccttccttgg cgttgataac
      601 cttggcacag atagccattc cgccaaacac cttaagggga accttaaaga gaatctcacg
      661 gtccttaaag gttccaggtt cgccctcgtc ggcattgcaa accacgtact tttccggatc
      721 agcctgctcg ctggtaaact tccatttaag ggcagttggg aaaccggcac ctccccttcc
      781 tttaagccct gattcaagta gctggttgat aatctcgtcg ttggaatgct ggtatgccga
      841 tttcaacacc tccttaaagt tgcaatcctg atcaaatatc aggtcaacac gtcgcaaaaa
      901 attactggcc atgattatag tctcctctac tttttcatgt aatcattaat gatttctcta
      961 actttttcgg gcgttagctc ggtgtaagcc tcctcgttaa caagcatggc cgggccctta
     1021 tggcaccagc ccaggcagtt ggtttcaagc agcgaaaact tacggttttg cgtagtttca
     1081 cc
//
LOCUS       DAOOQX010000753         1082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_193027_length_1082_cov_2.432327, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000753 DAOOQX010000000
VERSION     DAOOQX010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193027_length_1082_cov_2.432327"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLX53_10030"
     CDS             <1..185
                     /locus_tag="PLX53_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOY73463.1"
                     /translation="LTETLVMQPLASVCGYYFSHPKSSYFNIGRVTNDQLGDYARRLG
                     VSLEEAVRFFPTNVEQ"
     gene            302..>1082
                     /locus_tag="PLX53_10035"
     CDS             302..>1082
                     /locus_tag="PLX53_10035"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOY73464.1"
                     /translation="MKVIDILTKSRSTLFSFELLPPLKGHTIDTVFDTIDRLKEFSPA
                     YINITHHREDVVYMPNTAGEPVPVKVRRRPGTISLAAAIHQRYGIDVVPHLICGGFDA
                     HDIEDALIDLDFLGIQNILVLRGDADKISGRFEPKRGGHQHAVELVRQVQEFNRGIYL
                     EKLIENPKESDFCIGVGGYPEKHPEAPNIDVDIRYLKEKVDAGASYVVTQMFFDNAVY
                     FKFVERCREAGIDVPIIPGIKPISAKEQLTNLPRVFNVNIPQ"
BASE COUNT          285 a          238 c          258 g          301 t
ORIGIN      
        1 agctaaccga aaccctggtt atgcaacccc ttgcgtcggt ttgcggttac tacttctcgc
       61 atcctaaaag cagctacttc aatattggca gggttaccaa cgaccagctt ggcgattatg
      121 cccgtaggtt aggcgtaagc cttgaggaag ctgtaaggtt tttcccaaca aacgtcgaac
      181 agtgaatttt ttaaaacggt atagatattc ctgcaagtta gagttttatt tttttaactt
      241 tagtgcagaa tgttgaaatc cgggttgata ttttcccgtc acacttcaac aatccatcaa
      301 tatgaaggta atagacattc taacaaaatc gcgtagcacc ttattctcct ttgagctgct
      361 gcctccctta aagggtcata ccatagatac cgtttttgac acaattgaca ggctgaagga
      421 gttctcgcct gcctacatca atattaccca tcatcgagag gatgtggtgt acatgccaaa
      481 cactgcagga gagcctgttc cggttaaggt acgtagaaga cccggtacca ttagccttgc
      541 tgcagccatt caccagcgtt atggcattga tgtggttccc catcttatat gtggcggttt
      601 tgatgctcac gatattgaag atgccttgat tgatctggac ttcctgggta ttcagaatat
      661 cctggtgcta aggggcgatg ccgacaaaat ttccggcagg tttgaaccta agcgaggagg
      721 ccaccagcat gccgttgagc tggttcggca ggtgcaggag ttcaataggg gcatctacct
      781 ggaaaagttg attgaaaacc ccaaggagtc cgatttctgc attggagtag gcggttatcc
      841 cgagaagcac cccgaagcac ctaatattga tgttgatatt cgctacctta aggaaaaggt
      901 ggatgcgggt gccagctatg tggtaaccca gatgtttttt gataacgcag tatattttaa
      961 atttgttgag cgttgtcgtg aggctggcat agatgtccct ataattccgg gcataaaacc
     1021 tatcagtgcg aaggagcagc taacaaatct accaagggtt ttcaacgtca acattccgca
     1081 gg
//
LOCUS       DAOOQX010000754         1081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_193273_length_1081_cov_3.123782, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000754 DAOOQX010000000
VERSION     DAOOQX010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193273_length_1081_cov_3.123782"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLX53_10040"
     CDS             complement(<1..181)
                     /locus_tag="PLX53_10040"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOY73465.1"
                     /translation="MVGVTGGIGSGKSLVCSILARLGYPVYNSDIASRTITDSDEGVK
                     QRVKLLFGEEIYCNGK"
     gene            complement(187..>1081)
                     /locus_tag="PLX53_10045"
     CDS             complement(187..>1081)
                     /locus_tag="PLX53_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73466.1"
                     /translation="ILWFLLKLSHEYSDELVYPVRYSNLQKGKIIAGTPPEKISVKVK
                     AYGYTLLRYKFTSYSYPLNIDLKELKPNQGKNSSSQFCVSTTQQFSSISAQLGKDMDV
                     LDVFPDSICLELEDVVEKNVRVVSNLSLTFARQYMLAGNIEVEPGEITISGPAAILDT
                     INELKTQPVSLNLLAGNKKIILNIPQVKQVFMPNSEVEVFIPVEKFTEVMLNVPIIPL
                     NVPPSYDAILLPAEIQLRCNVTLSKYFKLKPSNFRAVCNISDTGSMEGNKLKVLLENH
                     PDFVSRVSFEPLYVDYILKKK"
BASE COUNT          297 a          228 c          238 g          318 t
ORIGIN      
        1 gcttgccgtt gcagtaaatt tcctctccaa aaagcagctt cacgcgctgc ttcactcctt
       61 catcgctatc agtaattgtc ctggaggcta tatcagaatt atagacagga tatcccaatc
      121 tggcaagtat gctgcaaacc agcgattttc cgcttccgat tcctccggtt accccaacca
      181 tcaggctcat ttcttcttca gaatatagtc aacatacaag ggttcaaagc taacccgact
      241 gacaaaatcg gggtggtttt cgagtaaaac ctttagcttg ttgccctcca ttgaaccggt
      301 gtcggatatg ttgcataccg ccctgaaatt tgaaggtttt aacttgaagt acttgctcaa
      361 ggttacgtta cacctaagct gaatttcggc cggtaataaa attgcatcgt agctgggagg
      421 tacgttaagg gggataatcg gaacattaag cataacctcc gtaaactttt caacgggtat
      481 aaatacctca acctccgaat ttggcatgaa aacttgtttt acctggggaa tgttcaatat
      541 aattttcttg tttccagcaa ggagatttag gcttacgggt tgggttttga gctcgttgat
      601 ggtatcgaga atagcagcag gcccggaaat agtgatttca ccaggttcaa cctcaatgtt
      661 tccggcaagc atgtactgtc gtgcaaaagt caggctaagg ttggaaacaa ccctaacatt
      721 tttctccaca acatcctcaa gttcaaggca aatggaatcg gggaaaacgt ccagcacgtc
      781 catatcctta ccaagttgcg ctgaaatgga agaaaattgt tgtgtagttg aaacacaaaa
      841 ttggctggat gaatttttgc cttggttagg ttttaattct tttaaatcaa tattaagggg
      901 gtagctgtag cttgtaaact tatacctaag gagggtatag ccgtatgctt taacctttac
      961 gctaattttc tcgggggggg tgcctgcaat aatttttccc ttctggaggt ttgaatacct
     1021 tacaggatat accagctcat cggagtactc gtggctaagc tttagaagga accagagtat
     1081 g
//
LOCUS       DAOOQX010000755         1079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_193909_length_1079_cov_2.786133, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000755 DAOOQX010000000
VERSION     DAOOQX010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193909_length_1079_cov_2.786133"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PLX53_10050"
     CDS             complement(<1..667)
                     /locus_tag="PLX53_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HOY73467.1"
                     /translation="MDIAFHPITVGTVVGLLVLILLLIISGLMSGSESAYFSLSPADL
                     KKLESKSHQRAQSFVLINLNESERLLATILIVDNLVNVGIVILSTYLTNSLIDFSNAP
                     VAGFIFQVIVITFILLLFGEVMPKILATNRALKFALFMAYPLHLLVRLFRPLSSLLLS
                     STSRIGKRASTHNQISMDELSNALEITSDSIAEEKQILQGIVTFGSTIVSEIMKPRID
                     VVAV"
     gene            complement(831..>1079)
                     /locus_tag="PLX53_10055"
     CDS             complement(831..>1079)
                     /locus_tag="PLX53_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOY73468.1"
                     /translation="AEKYVRSGKLLYVEGRIRTSSYDDKEGNKRYSTEIVCDNFRFLG
                     PNVNQQEHSTPYPEQVSDAKSNLSDVENIPSPDDDLPF"
BASE COUNT          316 a          233 c          256 g          274 t
ORIGIN      
        1 ccaccgccac tacgtcaatt cggggcttca taatctcgct cacaatggtg cttccaaacg
       61 ttacgatacc ctgaaggatt tgtttctcct cggcaatgct atcggatgtt atctccaggg
      121 cgttgcttag ctcatccatg gaaatctggt tatgggtgct cgcacgtttc ccaattctag
      181 aggttgaaga tagcagtagg gagctaaggg gcctaaacag cctaacaagc agatgaaggg
      241 gataggccat aaacagggca aacttaagcg cacggtttgt agcaagtatt ttaggcataa
      301 cctcgccgaa aagcagcaga ataaaggtga tgacaataac ctgaaaaatg aaaccggcaa
      361 cgggagcatt cgaaaaatca attaaagaat tagtgaggta ggtggaaagg atgacaattc
      421 caacattcac taggttatca acaatcagaa ttgtagctag caacctctcg gattcgttaa
      481 ggttgatcag cacgaaactc tgagcccttt ggtgcgattt gctttccaac tttttcaaat
      541 cggccgggga gagtgaaaag taggccgact cgcttccgct catcaggccg gaaatgataa
      601 gcaaaagaat caacacaagc aggccgacta ccgttccaac agtaatagga tgaaaggcaa
      661 tatccattaa tccaactcct tttaatattc gccattctgc gatggcttcg acttgtaaaa
      721 ttactaaaat attcagagat tacgttaatt tagcatacag cagccattta ccaacaaaaa
      781 accgatagtt tggttaggct atcggttttt ggggcaggga taataagcag ctagaatggc
      841 aggtcgtcat cgggcgaagg aatgttctcc acgtcggaga ggttggattt ggcatcagac
      901 acttgctcag ggtaaggggt ggaatgttcc tgctggttca cattggggcc aaggaatcga
      961 aagttgtcgc aaacaatctc tgtgctgtat cgcttatttc cctccttgtc gtcgtaggat
     1021 gaggttctaa tgcgaccttc aacatacaac agctttccgg acctgacata tttttcagc
//
LOCUS       DAOOQX010000756         1078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_194212_length_1078_cov_3.088954, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000756 DAOOQX010000000
VERSION     DAOOQX010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194212_length_1078_cov_3.088954"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1078)
                     /locus_tag="PLX53_10060"
     CDS             complement(<1..>1078)
                     /locus_tag="PLX53_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOY73469.1"
                     /translation="TSCSQLVEEGMRIKTHSSRVVKARKTLVELLLANHPDDCLYCER
                     NGRCELQSLAVELHVRERHAQNKTFSQKLDLSSHSIIRDPSKCVLCGRCVRICNELQS
                     VSTFEFVKRGCNMYIGTAMSRDLTFSNCIACGQCVMSCPTGALSERSNVDEVIDALNN
                     PNLIPVVQVAPSISVSLASELGLKPGKDLNGVLNAILRKIGFRYVFDSAAAADIAVME
                     TATELLKRRENGEKHPLISSCCPAWVKFMEQYYPEHLSWLSTTKSPQQVMGALIKSYF
                     APSYGINPAGIFSVSVMPCLAKKIEARREEMTTKGVSDVDTVISTRELVQLVELYGID
                     VQNVESQQPDSPFDIRSSAGKLFGV"
BASE COUNT          289 a          266 c          255 g          268 t
ORIGIN      
        1 aacgccgaaa agcttacctg ccgaactacg aatatcgaag ggggaatcgg gttgttggga
       61 ttcaacattc tgtacgtcaa ttccgtacag ttctaccagc tgcacaagct cacgggtgga
      121 aataaccgta tcaacatccg aaacaccctt ggtagtcatc tcctcccgtc gggcttcaat
      181 tttcttagcc aggcagggca ttactgaaac cgagaaaata cctgccggat taatgccgta
      241 ggatggtgca aaatacgatt taataagcgc ccccatcacc tgctggggtg atttagtagt
      301 ggatagccat gaaaggtgtt cggggtagta ctgctccata aactttaccc atgccggaca
      361 gcatgacgat ataagcggat gcttctctcc attttccctc cgcttcaaga gttcagtagc
      421 tgtttccatc accgcaatat cggccgcagc agcactgtcg aaaacgtatc taaagccaat
      481 tttacgtaga atggcgttta gcaccccatt caaatcttta cctggtttga gccccagctc
      541 gctagcaagc gatacggaaa tagatggggc cacctgaaca accggaataa ggttggggtt
      601 gttaagggca tctataacct catcaacgtt tgagcgttcg cttaacgcac cggtaggaca
      661 tgacattaca cactgcccac aagcaatgca gtttgagaag gttaaatcgc gcgacatggc
      721 tgtgcctatg tacatattgc aaccccgctt aacaaattca aacgtggata cagactgaag
      781 ctcattgcaa attcgaacac aacgcccaca aagcacacac ttggatggat cacgaatgat
      841 gctgtggctt gataggtcga gcttttggga gaaggttttg ttttgggcat gccgttcgcg
      901 aacatgcagc tcaacggcaa gggattgtag ctcgcagcgt ccgtttcttt cgcagtaaag
      961 gcaatcgtcc gggtggttgg caagtagcag ttcaaccaac gttttacgtg cttttaccac
     1021 tcttgatgaa tgggttttaa tccgcatgcc ctcctcaaca agttgcgaac atgacgta
//
LOCUS       DAOOQX010000757         1077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_194548_length_1077_cov_3.366928, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000757 DAOOQX010000000
VERSION     DAOOQX010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194548_length_1077_cov_3.366928"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1077
                     /locus_tag="PLX53_10065"
     CDS             <1..>1077
                     /locus_tag="PLX53_10065"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOY73470.1"
                     /translation="AIAREQVEGGAQAIDVCMDGALINAPVAITEFLNLVASEPDIAR
                     VPVMIDSSRFEVIEAGLKCTQGKSIVNSISLKEGEQEFIRRAKLIRRYGAAVVVMLFD
                     EEGQATTTEHRCRVAMRSYNLLVQTVGFAPEDIIIDPNILAIGTGMAEHSSQAVSFIE
                     TTKWIKQNLPYAGVSGGVSNLSFAFRGNNQIREAIHTVFLYHAVNAGMNMGIVNPGML
                     RIYTDIEPDLLRLTEDLVLNRRHDATERLLAYAHNVKDDKTDDKALPEWRSYPPGKRL
                     EHAIIKGNEDYVEVDTLEAFSIMGSAISVIEGPLMDGMKEVGTLFGSGRMFLPQVVKS
                     ARVMKKAVGILEPYISSSDESSKI"
BASE COUNT          281 a          227 c          295 g          274 t
ORIGIN      
        1 agccattgct cgtgaacagg ttgagggtgg ggcgcaggcc atcgacgtgt gtatggatgg
       61 cgccctgatt aatgcccctg ttgcaattac cgagtttctt aacctggttg catccgaacc
      121 ggatattgca agagttccgg ttatgattga ttcatcccgc ttcgaggtta ttgaagcggg
      181 cctcaagtgc acacagggca aaagcattgt gaattcaata agcctgaagg agggtgagca
      241 ggagttcatc cggagggcta agcttatcag gcggtatggt gctgcagttg ttgtcatgct
      301 ttttgatgag gaagggcagg caacaaccac cgaacatcgc tgcagggttg ccatgcgctc
      361 ctacaacctt ctggttcaaa cagtgggctt tgcccccgag gatataatta ttgaccccaa
      421 cattctggct attggaaccg gaatggccga gcactcatcg caggcggtca gctttattga
      481 aaccaccaaa tggattaagc aaaacctacc ctatgcagga gttagcgggg gtgtgagcaa
      541 cctttcgttt gcatttcgtg gaaacaacca gattcgggag gcgatacata cggttttcct
      601 ttaccatgct gtaaatgccg gtatgaacat gggaattgtg aaccctggca tgctccgcat
      661 ttacaccgat attgaaccag atttgcttag gttgacggag gatttggtgc ttaaccgtcg
      721 ccatgatgca accgaacggc tactagccta tgcccataat gtaaaggatg ataagacaga
      781 tgataaagct ttgccggaat ggaggagtta cccacccggt aaacgccttg agcacgccat
      841 tataaaggga aatgaggatt acgttgaagt tgatacgctt gaggccttta gcataatggg
      901 gtctgcaata tccgttattg aagggccttt aatggatggc atgaaggaag ttggcaccct
      961 ttttggctca ggtagaatgt ttcttccgca ggtggtgaag agcgcaaggg ttatgaagaa
     1021 agctgtcggt attctcgaac catatataag tagttcggat gaaagtagca aaattca
//
LOCUS       DAOOQX010000758         1077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_194558_length_1077_cov_3.095890, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000758 DAOOQX010000000
VERSION     DAOOQX010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194558_length_1077_cov_3.095890"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PLX53_10070"
     CDS             <1..757
                     /locus_tag="PLX53_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOY73471.1"
                     /translation="YYEYPRRISATELAKSLGITVEAVAKRGENLHEVNPMMGHRGVR
                     LGITYPEVTEMQVRAVFEATAELLKEGKSPHPEIMVPVTCEVAELDFTRKIVDRVYAE
                     VCQKFGMKSIEFKYGTMIEIPRACLLADEIARTTEFFSFGTNDLTQMTFGFSRDDAGG
                     FLPDYLDKKLLPADPFQTIDQKGVGQLIQMAVEKGRSTRPELKVGICGEQGGDPASVE
                     FCFKTGLTYVSCSPFRVPIARLAASQASIKYGK"
     gene            818..>1077
                     /locus_tag="PLX53_10075"
     CDS             818..>1077
                     /locus_tag="PLX53_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOY73472.1"
                     /translation="MSRQKSKLIRVIIQLFLLILPATWLWAQDDELLPTDTADFKSHY
                     NMLLIFEADSGHTDMVQYLLELGADPNTQTEQGVTPLMYASQ"
BASE COUNT          274 a          263 c          261 g          279 t
ORIGIN      
        1 gtattacgag tacccgcggc ggatcagcgc aacagaactt gcaaaatcgc ttggaattac
       61 tgttgaggct gtagctaagc gtggcgaaaa cctgcacgag gttaacccca tgatgggaca
      121 taggggagtt cgtttgggaa ttacctatcc ggaagttacc gaaatgcagg tgcgtgctgt
      181 ttttgaagct actgctgaac tactaaagga aggcaaatca ccacatcccg aaattatggt
      241 tccagtaacc tgcgaggttg ccgaactcga cttcacccgt aagattgttg accgtgttta
      301 cgccgaagtc tgccaaaagt ttggaatgaa atccatcgaa ttcaagtatg gcaccatgat
      361 agaaataccc cgtgcatgcc ttctggccga tgagattgcc cgtaccacgg agttcttctc
      421 ttttgggaca aacgacttaa cccaaatgac atttggattc agccgcgacg atgcgggtgg
      481 tttccttccc gattacctgg ataaaaaact tttgcctgcc gatccgttcc aaaccattga
      541 ccaaaaaggt gttggccaac ttatacaaat ggcggtagaa aagggtcgca gcactcgccc
      601 cgagcttaag gtgggtatct gtggcgaaca gggaggtgat ccggcttcgg ttgagttctg
      661 cttcaaaacc ggccttacct atgttagctg ttcacccttc agggttccta tagctcgtct
      721 tgcagcttcg caggcaagca ttaagtatgg aaaatagttt tactgttttt tcctaccaac
      781 gcctccagtt tggaggcgtt ttttttattt ttgtgccatg agcaggcaaa aatcaaaatt
      841 aatccgagtt attatccagc tattcctact tattctccct gccacctggt tgtgggcaca
      901 ggatgatgag ctgctgccaa cagatacggc agattttaaa tcacactaca acatgctgtt
      961 gatatttgag gctgactccg ggcataccga catggttcag tacctgctgg agctgggtgc
     1021 cgaccccaac acgcaaaccg agcagggggt aacacccctc atgtacgcct cgcaatg
//
LOCUS       DAOOQX010000759         1076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_194853_length_1076_cov_2.872674, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000759 DAOOQX010000000
VERSION     DAOOQX010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194853_length_1076_cov_2.872674"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PLX53_10080"
     CDS             complement(<1..824)
                     /locus_tag="PLX53_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOY73473.1"
                     /translation="MITRELINPQSIVVVGGSADVQKPGGKVLKNLIDHNFKGKLYVV
                     NPKADEVQGVRAYPDAEDLPPVELAILAIAAKFCPQAVETLAAKKGTKAFIILSAGFH
                     EESEEGARLEQQIVETINKAGGCLIGPNCIGVMNTNYAGVFTTPIPRFNTHGADFITG
                     SGATAVFIMESGISNGLQFNSVYSVGNSAQLGVEDILEYLDLTFDPNTSSRVKMLYIE
                     SIGQPQKLLKHASSLVRKGCRIAAVKAGSSSAGSRAASSHTGALASSDVAVDALFR"
BASE COUNT          273 a          250 c          227 g          326 t
ORIGIN      
        1 tttctaaata gtgcatcaac agccacatcg ctgctggcga gtgctccggt gtggcttgaa
       61 gcagcccggc ttcctgctga tgagcttcct gccttaacag ctgcaatccg gcaacccttt
      121 ctcacaagcg atgaggcatg tttaagaagt ttttggggtt ggcctatgct ttcaatgtaa
      181 agcattttta ctctggagct ggtgtttgga tcgaaggtta ggtccaggta ctccagaatg
      241 tcctcaactc ccagctgggc gctgttgcca accgagtaaa cgctgttgaa ctgaagtcca
      301 ttggaaattc ctgattccat gataaatacg gccgttgctc ccgaaccggt aatgaaatct
      361 gcgccatgcg tattaaagcg tggaatagga gtagtaaaaa caccggcata gttggtattc
      421 attaccccaa tgcagttggg tccaatcaaa catccaccag ctttgtttat cgtttcaact
      481 atttgctgct ccagcctggc accctcctcg ctttcctcat gaaacccggc tgataggatg
      541 ataaatgctt ttgtcccctt ttttgcagcc agcgtttcta ctgcctgtgg gcagaacttt
      601 gctgcgatgg ccagaatggc aagttcaaca ggtggtaaat cttcggcgtc aggatatgcc
      661 ctgacaccct gaacctcatc agccttgggg tttactacgt atagcttacc cttaaaatta
      721 tggtcaataa ggtttttcaa aaccttacca ccgggtttct gaacgtcggc agaacctccc
      781 actacaacta tactctgggg gttaatcagc tctctggtta tcataaaaaa gtattttaaa
      841 cctgtttttt caggatattt ttccggttgc aaacgtaatg ctaataaagc aatgtagcaa
      901 gcttcaattt gaaagtgaga acctataaaa atggactacg aactatgcca ttttattggt
      961 ttgcgatttc agccagtcaa tttcactatt gttcaacttt ttaaggtcaa aactttacta
     1021 gctgtcaagt ttactcatat tacccttatt tttaaggtat tcgtgattgc atggca
//
LOCUS       DAOOQX010000760         1076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_194865_length_1076_cov_2.795299, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000760 DAOOQX010000000
VERSION     DAOOQX010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194865_length_1076_cov_2.795299"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..258)
                     /locus_tag="PLX53_10085"
     tRNA            complement(185..258)
                     /locus_tag="PLX53_10085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(222..224),aa:Arg,seq:ccg)
     gene            complement(448..945)
                     /locus_tag="PLX53_10090"
     CDS             complement(448..945)
                     /locus_tag="PLX53_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOY73474.1"
                     /translation="MSQQEFNTALVELEPNLERFAYSLTANREDANDLLQETYLKALT
                     YKDKFEDNTNLKAWTFTIMKNTFINNYRKAVKQNTTFDSSENQFLLNSSYEKNGPESM
                     YSHSEISKKIDQLEDEFRIPFQMHTSGYKYKEIAEKLNLKIGTVKSRIFFSRQKLMSA
                     LRDYE"
BASE COUNT          260 a          218 c          234 g          364 t
ORIGIN      
        1 attcattgtt taacctctac aatatgccta ttcttagaag tctgctggca tttttagatg
       61 ggcaatgcgg gcttgaaagc tatcgcccga tgttggttta tcatggttga gcagaaacgc
      121 tctagcgaat gttgggagca tgttgttaaa gtaaaggtca accactacag gttgaccttt
      181 tctgtgaccc cggcgggatt caaacccacg actttcagaa ccggaatctg acgttctatt
      241 cagctgaact acggggccat ttcaggcagc aaatatagtg gagttttatg ctaattccaa
      301 cttttaccca catgatattc gataagtatc agcctttgag tgttttctaa atgtttcctt
      361 cgttttaaaa aggttttgac taaaaattgc agaagccatc aatctccgat ggctcctgct
      421 acctattatg tgttgttgcc tagcacctta ctcgtagtcc ctaagggcgc tcatcaactt
      481 ttggcgtgaa aagaaaattc tgcttttcac cgtgccgatt ttaaggttga gcttttctgc
      541 tatttccttg tacttgtagc ccgaagtgtg catctggaat ggaatccgaa attcatcttc
      601 aagctggtca attttcttgc taatttcgct gtgggagtac atggactcgg gtccattctt
      661 ctcgtagctg ctgttgagca ggaattgatt ttcgctgctg tcgaatgtgg tgttttgctt
      721 aaccgcctta cggtagttgt tgatgaaggt atttttcatt atcgtgaagg tccaggcctt
      781 taagttggtg ttgtcctcaa acttatcctt gtaggtcagc gccttcaggt aggtttcctg
      841 tagcaggtcg ttggcatcct cgcggttagc tgttaagctg taggcaaatc gttccaggtt
      901 tggttccagc tccactagtg cggtattgaa ctcttgctgt gacatatttg taggttttta
      961 gttactattc cttggttaaa cttcacgaca aagttagaat tagtctaaat aaaaatcaag
     1021 ttgataatta tcaactcacg ccaacattta acttaattct aaatagaaaa ttggac
//
LOCUS       DAOOQX010000761         1075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_195157_length_1075_cov_3.165686, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000761 DAOOQX010000000
VERSION     DAOOQX010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195157_length_1075_cov_3.165686"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PLX53_10095"
     CDS             <1..700
                     /locus_tag="PLX53_10095"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOY73475.1"
                     /translation="HTMAIQTLHQETGIDPSIVEVIVNTEAGVPTGMTTSSRATALVA
                     NALINASAAFKADLAEASLEQLAGRTYRGKFVCDWTSKPGAETENPVVHFAYGYAAQV
                     AILDDEGRLRKLVAAHDAGKIMNRMLFEGQIEGALHMGMGYALTEDFPMSEGYPTSYK
                     FNDIGIIRANEMPELKVIGIEEADPVGPYGAKGIGEIGLVPTAAAIANALMAYDGIKR
                     TKLPMRRSRIKDKE"
BASE COUNT          337 a          224 c          239 g          275 t
ORIGIN      
        1 tcacaccatg gcaattcaga cacttcatca ggaaaccggt atagaccctt cgattgttga
       61 agtaatagta aacaccgaag ccggtgttcc aacgggtatg acaacctcct caagggctac
      121 cgcacttgtt gccaatgcct taatcaacgc ttccgcagct tttaaagccg atttagctga
      181 agcttcactc gaacagctgg cagggagaac ctacaggggc aaattcgtct gcgactggac
      241 gagtaaaccg ggtgccgaaa ccgaaaaccc cgttgttcat tttgcttatg ggtatgcagc
      301 ccaagtggca atccttgatg atgagggacg tttaagaaaa ctagttgccg cccacgatgc
      361 cggtaaaatt atgaaccgta tgctctttga gggtcaaatt gaaggtgccc tgcacatggg
      421 aatgggctac gccctcaccg aagatttccc tatgagcgaa ggctatccta ccagctacaa
      481 attcaacgat attggaatca taagagctaa cgaaatgccc gagctaaagg tcataggaat
      541 tgaggaagcc gatcctgttg gcccttatgg tgcaaaagga attggtgaaa ttggcttagt
      601 acccaccgct gcagctattg caaatgcatt aatggcctac gatgggataa agaggacaaa
      661 gctacccatg agaaggtcca ggataaagga taaagaataa agaataaaaa aatggctcaa
      721 tttaaagaaa atgtttttcc ccgataacca taacgaaatc atgaatagta atctttgcgt
      781 tctgtaatat ctttttgtgc tttgtggttt ctagtattat ttaaccacaa agttcacaaa
      841 gaaaagttta acaaacttat cattatgagt gataatcggt ataacaacat tattttttta
      901 atagagccaa agagataaag ggtaaaagaa accttcggct aaaagggttt accctacaac
      961 aggtgttacg tcgagtagtt cggctggtgt tatagtgagc cagtcgaact actcactata
     1021 acacctgccg agctgctcag gacaggcttt tatagcttct catggattat aagtt
//
LOCUS       DAOOQX010000762         1074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_195574_length_1074_cov_2.520118, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000762 DAOOQX010000000
VERSION     DAOOQX010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195574_length_1074_cov_2.520118"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLX53_10100"
     CDS             <1..465
                     /locus_tag="PLX53_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOY73476.1"
                     /translation="HALNPKLTPLIPSDKKFKIYVSALTSMSYDGINRIPTTDNRLIR
                     RIVRDYRYRGYNALQTIARWESVRRGEDRNIFPYQEEADEMFNSALLFEFGLLKSYVE
                     PLLQEVPPNRPEFDEADRLLKFLYYFNTISDSEVPPTSIIREFLGGSAFSYS"
     gene            649..>1074
                     /locus_tag="PLX53_10105"
     CDS             649..>1074
                     /locus_tag="PLX53_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73477.1"
                     /translation="MARLFIVIINIIGITLAGILGGGNVLVTMKAPSRVDAGSEFDVE
                     VLLKKAGVDGFARFQQELPLGLTAELIESSLGDFTFEDQKVKVVWLKLPAAKELRFVY
                     RIRVDERLKVSFNLGGVFSYIENNERKTSSVQAAQISITP"
BASE COUNT          293 a          232 c          221 g          328 t
ORIGIN      
        1 catgccctta acccaaagct caccccgtta atcccatccg acaaaaagtt taaaatatac
       61 gtttcagcgt tgacctccat gtcgtacgac ggaattaacc gtattccaac caccgataac
      121 aggcttatca gaaggattgt ccgcgactac cggtacaggg gttacaacgc ccttcaaaca
      181 atagcccgct gggagagtgt ccgtagggga gaggatagga acatatttcc ctaccaggag
      241 gaggctgacg aaatgtttaa ctctgccctg cttttcgagt ttggccttct taagagctat
      301 gttgagcccc tgcttcagga agtgccgccc aatagacctg aatttgatga agccgaccgc
      361 ctgcttaaat tcctttacta tttcaatacc atttcagaca gcgaggttcc tccaacctcg
      421 attataagag aatttttagg gggtagcgct ttctcctact cttaaaatgc catgttaagt
      481 tattaacatg ctatatagtt gaaaatcaat tcaatgaaat tattttgttt atatattttt
      541 tgccttaacc tgctgcttcc catgattttt accttgttct cttgctttta attataatta
      601 ttcactataa ttgtaacaaa ttaaccctaa acctgaatcc aagctataat ggcaaggcta
      661 tttatagtta ttatcaacat aataggtatt acgcttgctg gcattttggg aggtggtaat
      721 gtgctggtta ccatgaaagc tccaagtcgg gtagatgcgg gttcggaatt tgatgtggaa
      781 gtgctcctaa aaaaggctgg tgttgatggt tttgcccgat ttcagcagga actaccccta
      841 ggcctcactg ctgaactaat agaatcatcc cttggcgatt ttacttttga ggatcaaaag
      901 gtaaaggtag tatggcttaa gcttcctgca gccaaggagt tacgatttgt ttaccgaatt
      961 cgggttgacg agcgtttaaa ggtgagtttc aaccttggtg gcgttttctc atatattgaa
     1021 aataacgagc gtaaaacttc ttccgtacag gcagcacaga tttctatcac ccct
//
LOCUS       DAOOQX010000763         1068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_197383_length_1068_cov_3.096742, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000763 DAOOQX010000000
VERSION     DAOOQX010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197383_length_1068_cov_3.096742"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1068
                     /locus_tag="PLX53_10110"
     CDS             <1..>1068
                     /locus_tag="PLX53_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73478.1"
                     /translation="TVLNILLHDLTLSNIVLNYADDSLRAKACVAIPEIKLNGKVMGS
                     YYAGAVEGSISVSDLALEGTHADRMGKTSLDFKVGYDNGDISVDKLNLNADGFGLSAN
                     GKAKLSDSIFVDMGVGLTNMDFGRLTAYLPDTLLSSYGLTALGGIFDLKAHIKGFVYD
                     TLLLPSVEASLDFRNGFIKTTSYPTVETLAFNGKVKLPDPNNLKTASADFSTFRVKTH
                     ASNLNLAFKILNIEKPIYNVKGDMFITLDEFGSFIPDSLVKSLSGNIIARIETRGQLP
                     DSLGMSSADYFMQNTTLNVKLRNITAVLDSSTSVRGVNLNFDFLPGKQVAVENFTADL
                     SIPGYHVGLDNFLLRARLIGDI"
BASE COUNT          270 a          276 c          228 g          294 t
ORIGIN      
        1 accgttctga atattctcct gcacgacctt acactcagca acattgttct taactatgcc
       61 gacgatagcc tgagggctaa ggcttgtgtt gctattcctg aaattaagct caacggaaaa
      121 gttatgggca gctactatgc aggggcagta gaggggtcaa tttccgtttc tgatttggct
      181 cttgagggta ctcatgctga cagaatgggt aaaacttctc ttgacttcaa ggtagggtac
      241 gataatggcg atatttctgt tgataagctc aatttgaacg ccgatggatt tgggctgtct
      301 gcaaatggca aggctaaact ttccgactca atttttgtag atatgggtgt gggcctcacc
      361 aatatggatt ttggcaggct gactgcctat ctccccgata ctcttctctc cagctacgga
      421 ctcactgccc ttggaggcat tttcgacctt aaagcccaca tcaaaggttt tgtttacgac
      481 accctcttgc tcccctcagt tgaggcaagt ttggatttcc gaaatggatt catcaaaacc
      541 acctcgtacc ccactgtcga aacactagct ttcaacggca aggttaagct acccgacccc
      601 aacaacctta aaactgccag cgctgatttt agcacattca gggttaaaac acacgccagc
      661 aacctgaacc tggctttcaa gatattgaat atcgaaaaac ccatctacaa cgttaagggc
      721 gacatgttca ttaccctcga tgaatttggc agctttattc ccgatagcct tgtcaagagc
      781 ctttcgggaa acatcatagc ccgcatcgaa acccgcgggc agctgcccga cagcctgggg
      841 atgagcagcg ccgattactt catgcaaaat actactctta atgttaagct tcgcaacata
      901 accgctgtgc tagactcttc aacctctgtc cgtggtgtaa accttaactt cgactttctt
      961 cccggaaagc aggttgctgt cgaaaatttc acagccgatt tatccattcc gggttaccat
     1021 gtcggcctcg acaattttct gctacgtgcc aggctcattg gcgatatt
//
LOCUS       DAOOQX010000764         1068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_197462_length_1068_cov_2.426456, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000764 DAOOQX010000000
VERSION     DAOOQX010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197462_length_1068_cov_2.426456"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PLX53_10115"
     CDS             complement(<1..1027)
                     /locus_tag="PLX53_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73479.1"
                     /translation="MEMSFDLKWIDYLVMIIYFVFVLGIGWALKKFMKDANAFLEAGR
                     SMPAWVAGLAFISTNLGALEVMGMTGSGAKYGMLTTHFYWVGAIPAMVFLAMFMMPFY
                     YGSKARSVPEYLKLRYDEKTRGLNAILFALMTVLSSGISMYALAILTEKVLGWNFHVS
                     LLLSAAIVLGYTYLGGLSSAIYNEVLQFFIIVIGLLPLVFLSLHQVGGWEGLQSQLAP
                     VAASHGYEPDAYTTMWRHSGTAGGNPLGVEWYGILLGLGFVLSFGYWCTNFLIVQRAM
                     AAESPRAARNTPLIGAIPKMFIPFLIIVPGMVALALMNNPAAQFTIPTNEAGQPIYDY
                     TIISLLKY"
BASE COUNT          305 a          287 c          236 g          240 t
ORIGIN      
        1 agtatttcaa cagcgagata atggtgtaat cgtaaattgg ctgacctgcc tcgtttgtag
       61 gaatggtaaa ctgagcagca gggttattca taagcgccag cgcaaccatg ccgggaacga
      121 taatcaggaa tggtataaac attttaggga tggcacctat aaggggagta ttccttgctg
      181 ccctgggcga ttcggcagcc atggcccgtt gaacaatcag gaagttggtg caccagtacc
      241 caaacgaaag cacaaaaccc aaccctagca gtattccata ccactcgacc ccaagggggt
      301 ttcccccggc tgtccccgag tgcctccaca tggtggtgta ggcatcaggc tcatagccat
      361 gactcgctgc tacaggtgcc agctgggatt gcaaaccctc ccaacctcct acctggtgca
      421 agcttaaaaa tactagcggt agcagaccaa tcacaatgat aaaaaactgc agcacctcgt
      481 tgtagatggc tgacgacaaa cctcccagat aggtgtagcc cagcacaatg gctgccgaca
      541 aaagcaggct tacatggaaa ttccacccca acactttttc ggttaaaatt gccagcgcat
      601 acatggatat ccccgaggat agcacggtca taagggcaaa cagtatggca ttcaaccccc
      661 ttgttttttc atcgtagcgc agcttaaggt attccggaac cgaacgcgcc tttgaaccgt
      721 agtaaaaagg catcatgaac atcgccagaa ataccatggc cggaatggcc ccaacccagt
      781 aaaaatgggt ggtgagcatt ccatactttg cgccgctacc ggtcatcccc ataacctcca
      841 atgccccgag gttggtagat atgaatgcca aaccggctac ccatgccggc atagagcgtc
      901 cggcctcaag aaaggcgttg gcatccttca taaacttttt taatgcccat cctatcccaa
      961 ggacaaacac aaaatagatt atcattacca gataatctat ccacttaaga tcaaaactca
     1021 tctccatcgt ttcagcagtt tttgttgaac gtcgtgttac gtactatt
//
LOCUS       DAOOQX010000765         1067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_197690_length_1067_cov_3.616601, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000765 DAOOQX010000000
VERSION     DAOOQX010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197690_length_1067_cov_3.616601"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLX53_10120"
     CDS             complement(<1..272)
                     /locus_tag="PLX53_10120"
                     /inference="COORDINATES: protein motif:HMM:NF025652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4372 domain-containing protein"
                     /protein_id="HOY73480.1"
                     /translation="MGKDTAKNLASQPVFKQVMKILPGEQFDIVVRQCGSDCCYKSFF
                     SWDQLVVMLFGIFSRCDSMGEVCDGMRALGGKLNYLGMDSAPSKSTA"
     gene            831..>1067
                     /locus_tag="PLX53_10125"
     CDS             831..>1067
                     /locus_tag="PLX53_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009235252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOY73481.1"
                     /translation="MALLDELSEQEVIRRQSLDELRKLGIDPYPAAMYPVTHTTGSIL
                     SNFENLCEQQAEVCIAGRIMARRIMGSASFLELQD"
BASE COUNT          279 a          264 c          234 g          290 t
ORIGIN      
        1 gccgtgcttt tggacggtgc actgtccatc ccaaggtagt tgagctttcc tcccaaagcc
       61 cgcatcccat cacatacttc gcccatcgaa tcgcagcggg aaaaaatccc gaatagcatc
      121 accaccagct gatcccatga aaaaaaggac ttgtagcagc agtcgctgcc gcactgcctc
      181 actacaatat caaattgctc tcccggaaga attttcataa cctgtttaaa aaccggctga
      241 ctggctaaat tctttgctgt atctttgccc atgttgcagt ttttttagca aaataaactt
      301 acaacttcgg ggcgaaacct cgcgggagta gtcccttttt cactgaaatt ttaaccggtc
      361 agtagagact taaaactaaa attttaggtt tagaaccgaa taaaagtcca ggttttcagg
      421 aacaaaggaa tataggattt taaatcgagt aggtaggggg gattactcct cgccccctta
      481 caccaccgtg catccgcctg cggcggacac ggcggtttcc tttaatggtg caatgctttg
      541 tagtgtaatg tgaaatgata atctgtataa accaagttct ttgaaccagc tagttcccgt
      601 agcctcatgg gcttggggag tgccttgctt ttgccaccat cctttacccg aaagcgtaag
      661 tatccagcat tgccgcgtcc tgaccccctg acgtggtaga aactgctgta acgagtaaat
      721 ccgttagcac tgttttaatc cggcttgttg atcattgatt cggtacattt tcttctggtt
      781 ataaaaaatt cgggtaaatt tgcacggttt ttacctaatc acctattagt atggctttac
      841 ttgacgagct gagcgagcag gaggtaattc gtaggcaatc actagatgag ctgcggaagc
      901 tgggaataga cccctaccct gctgccatgt atccggttac ccacacaacc ggcagcatac
      961 tttccaactt cgaaaacctt tgcgaacaac aagctgaggt ttgcattgcg ggccgaatca
     1021 tggcacgcag aattatgggc agcgcttcgt tccttgaact tcaggac
//
LOCUS       DAOOQX010000766         1064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_198816_length_1064_cov_2.038652, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000766 DAOOQX010000000
VERSION     DAOOQX010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198816_length_1064_cov_2.038652"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..468)
                     /locus_tag="PLX53_10130"
     CDS             complement(13..468)
                     /locus_tag="PLX53_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOY73482.1"
                     /translation="MSLEERISQDIKTAMLAQDKVRLDALRAIKSAILLAKTEGEHKD
                     TLAPESEARLLQKQYKQRKESAEQYVQAGRQELADKELAEAAIIEEYMPKMLSADELK
                     AELVKIIGQVGAKAPSDLGKVMGVASKLLAGRADGKAISDMAKQLLGGS"
     gene            672..>1064
                     /locus_tag="PLX53_10135"
     CDS             672..>1064
                     /locus_tag="PLX53_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73483.1"
                     /translation="MRLRILLIFIAAFPFQNAFCQKSSYFKRVFVDAEYYLLYEEYRD
                     ALPLYQEIYKAYPSNCNLAYRIGLCYLNIPNEKKKSLPYFEKAITSITPSYQEGYFTE
                     TKAPREAFLRYGQALRIDSQFDKAMEAFK"
BASE COUNT          266 a          268 c          207 g          323 t
ORIGIN      
        1 accggtagag gattagctac cacccagaag ctgtttagcc atgtcggaaa tagcctttcc
       61 atcagccctg ccggctagca gcttgctggc aacacccata acttttccca ggtccgaagg
      121 tgccttagcg ccaacctgcc caattatttt taccagctca gccttcagct catcagcgct
      181 aagcattttg ggcatgtact cctctatgat ggcagcttcg gctagctcct tgtcggccag
      241 ctcctgccgg ccagcctgaa cgtactgctc agccgactcc ttcctctgct tgtattgctt
      301 ttgcagcaac ctagcctcgg attcaggagc aagcgtatcc ttatgctcgc cttctgtttt
      361 tgcaagcaaa atggcacttt ttattgcccg tagcgcatcc agcctaacct tgtcctgtgc
      421 cagcatggct gttttgatat cctggctgat tctttcctct aacgacatct cctcgcaaat
      481 tatggtttct gacacaaagt tagaaaaaat tggaataatt tgactttaac tatttatttg
      541 aattaaaata atttactact tttgaaagtt gtttagctat ttgtcatatc ttgctggttg
      601 ttttaaaatt gaggtgttat ttctaaacaa tttggttgga taaatccttt agggttgtaa
      661 actgccgatt gatgagactg aggattttgt tgatatttat agctgccttt ccattccaaa
      721 atgcgttttg tcagaaaagc tcctacttta aaagagtttt tgttgatgcg gagtactacc
      781 tgctttacga ggagtatcgt gacgcgctac ctctttacca ggaaatatac aaggcctacc
      841 cttcaaactg taacctggcc taccgcatag ggctttgcta cctcaacatt cccaacgaaa
      901 aaaagaaatc cctaccctat ttcgagaagg ctattacctc catcacccca tcataccagg
      961 aaggctattt caccgaaacc aaagcaccgc gtgaagcttt cctacgttac ggacaagcct
     1021 tacgaattga ctcgcagttc gataaggcca tggaggcttt taaa
//
LOCUS       DAOOQX010000767         1060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_199928_length_1060_cov_3.585075, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000767 DAOOQX010000000
VERSION     DAOOQX010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199928_length_1060_cov_3.585075"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..917)
                     /gene="tatC"
                     /locus_tag="PLX53_10140"
     CDS             complement(117..917)
                     /gene="tatC"
                     /locus_tag="PLX53_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOY73484.1"
                     /translation="MAGKASKHNEMTFWQHLGELRNIFIRCMLAVVVLSIVAFCLKDI
                     IFQQVVLRPRMPEFVTNRLLCRLAEQWNSPVLCINSTILPLINIELAGQFTVHLRVAF
                     WSGVIMAFPYIMYELAKFFRPALKQGESAMLRGVALMFTLCFAIGAAFGYFIIAPISI
                     HFLGTYSVSAQVVNQITLGSYLSTVLGLIFAGGAAFEMPVVVLYLSRLGVVKPQFLSR
                     YRKVAIVVILVISAVITPPDVFSMTIVAIPLLLLYEVSVLLARMEFKD"
BASE COUNT          345 a          254 c          217 g          244 t
ORIGIN      
        1 ttgagcgtca ataaccctac ccctgatttt ggttatctgc ccctgggcaa aaatggatga
       61 aacaaatgcc agctgaagtg ctagaaaaaa caacaatctc ctcataatca taaaaatcaa
      121 tccttaaact ccattcgggc cagcaaaacg ctcacctcgt agagaagcaa aagcgggatg
      181 gcaacaatgg tcatgctgaa cacatcggga ggtgttatca cggccgaaat aaccaggata
      241 actacaatgg caacttttct atatcgggat aggaattggg gtttcaccac tcctaaacgg
      301 ctaaggtaca gcacaactac cggcatctca aaggctgcgc cacctgcaaa aatgaggcct
      361 aaaacggttg acaggtatga acccagagtg atttggttga ctacctgcgc actcaccgag
      421 taggttccta aaaaatgaat ggatatgggt gctattatga agtacccaaa agcagcgcca
      481 attgcaaagc aaagggtaaa cattaatgct acacctctaa gcatagctga ttccccctgc
      541 ttaagcgccg gtctgaaaaa tttggcaagt tcatacataa tatacggaaa agccataatt
      601 accccgctcc agaaagccac ccttaaatga acagtaaact gcccagcaag ttcaatgttg
      661 ataagcggta aaatagttga gttgatgcaa agaaccggag agttccactg ctcagccaac
      721 cggcataaca accggttggt aacaaactcg ggcattcgag ggcgaagtac aacctgctgg
      781 aaaataatgt cctttaagca aaatgccaca attgaaagca caacaactgc caacatgcag
      841 cggataaaaa tattcctcaa ttcacccagg tgctgccaga aagtcatttc attatgtttc
      901 gatgcttttc cagccatcac cttaaggaaa ttagactata ccttctctga gaatatcgtg
      961 aatgtgtatc accccttcat atcggtcctc atgggtaacg agcagctggg tgattttatt
     1021 ctgctccata aggttgaaag ccgtaattgc caactcctcc
//
LOCUS       DAOOQX010000768         1059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_200325_length_1059_cov_2.738048, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000768 DAOOQX010000000
VERSION     DAOOQX010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200325_length_1059_cov_2.738048"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /gene="ung"
                     /locus_tag="PLX53_10145"
     CDS             <1..569
                     /gene="ung"
                     /locus_tag="PLX53_10145"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOY73485.1"
                     /translation="YRVYPPGSKIFAAFDFTPFTSVKVVLLGQDPYHGHGQAQGLCFS
                     VPRGIPKPPSLVNIFKELHDDLGLAIPPHGNLEAWARQGVLLLNATLTVRANQAGSHQ
                     HKGWEQFTDAAIRALAEQRENLVFMLWGNYAQAKAALVDGHKHCILKAPHPSPFSANQ
                     GFFGCRHFSRANDFLVRAGKEPVDWQIS"
     gene            complement(636..>1059)
                     /locus_tag="PLX53_10150"
     CDS             complement(636..>1059)
                     /locus_tag="PLX53_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73486.1"
                     /translation="DIPDSYTHITTGLGVSNPSCIVLTPLVTEKEEALGVMEISSYSP
                     ITDIQVEFMKQACRNLASSLILHKAIKANEQIIAQMKVKTEELLANEEELRQNMEELT
                     AIREDMERREKDLNGQIAELKYKLRLHGIIFEADTYHN"
BASE COUNT          235 a          261 c          262 g          301 t
ORIGIN      
        1 agtacagggt ttaccctccc ggctctaaaa tctttgctgc atttgacttt accccattca
       61 ccagcgtgaa agttgtcctg ctgggtcagg atccctacca tgggcatggg caggcgcaag
      121 gactctgttt ttctgtcccc agaggcatac ctaagcctcc atcgctggtg aacatattta
      181 aggagctgca tgacgatttg ggtttagcca ttcctcctca tggtaacctg gaggcatggg
      241 caaggcaagg tgttcttttg ctcaatgcta cccttactgt gagggcaaac caggccgggt
      301 cgcaccagca taagggttgg gagcagttta ccgatgccgc aataagagca ttggccgagc
      361 aacgcgaaaa cctggttttt atgctatggg gaaactacgc acaggctaaa gcggcattag
      421 ttgatgggca taagcattgc atactgaaag ccccacaccc atcgcccttc tcagccaacc
      481 agggtttttt cggttgcagg cacttctcca gggcaaatga ttttttggtt agggcgggga
      541 aggaaccagt tgactggcaa attagctagt tgcatttact tttagcgctg ggcagttgaa
      601 aagtctatac tcctccaccc actggcgtgt gcagcctaat tgtgataggt atcggcttca
      661 aaaataatcc catgaagcct tagcttgtat ttaagctcgg caatttgtcc gtttaggtct
      721 ttttcccgtc gttccatgtc ctccctgatt gcggtcagct cctccatgtt ctgccttagc
      781 tcttcctcgt tggccagtag ctcctcagtt tttaccttca tttgggctat aatctgttca
      841 ttggccttaa ttgccttatg aagaatcaac gaggaggcga gatttcggca tgcttgtttc
      901 ataaattcaa cctgtatgtc ggttatgggg ctgtagctgg aaatctccat aacccccaac
      961 gcctcttcct tttcggttac cagtggggta agaacaatgc agctggggtt cgaaacgcct
     1021 aaaccggtgg tgatgtgcgt gtagctatca ggtatatcg
//
LOCUS       DAOOQX010000769         1054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_202168_length_1054_cov_1.244244, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000769 DAOOQX010000000
VERSION     DAOOQX010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202168_length_1054_cov_1.244244"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLX53_10155"
     CDS             <1..234
                     /locus_tag="PLX53_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOY73487.1"
                     /translation="SSNGNGTDDYGFSALPGGYRYANGTFGYIGYEGYWWSSTEYGPD
                     IVGTRYMRYNYGTVSRSHLSKSWGFSVRCVRDL"
     gene            complement(762..>1054)
                     /locus_tag="PLX53_10160"
     CDS             complement(762..>1054)
                     /locus_tag="PLX53_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOY73488.1"
                     /translation="MEADPSKLKHRNSFNVTAMSFEPSQVYSAIRRRIPDFTMEYSVD
                     PVKKAIADSWPNYMDDSCARQEWGWSPKWNLETMTDDMLRAIGEKMKKGLLG"
BASE COUNT          243 a          235 c          282 g          294 t
ORIGIN      
        1 agctccaacg gaaacggcac cgacgactac ggcttttcgg ctcttccggg gggctaccgt
       61 tacgccaatg gtactttcgg ctacattgga tacgagggtt actggtggag ttctaccgag
      121 tacggtcccg atatcgtcgg gacccgctac atgcgctaca attacggcac tgtgagcagg
      181 agccacctca gtaagagttg gggtttttct gttcgttgcg tgagggattt ataggttatt
      241 tgactatttg aaactttggc tatttggggt atggggcgaa gccccatcga atttttgcga
      301 aaagtgaggg aagaaccgaa cttgttcgag ttatgccgag ctaagcaaaa atcacgtagt
      361 aatttttttt gaaaaatggt actttatgat gagctgccct cgctcgccta tgtctgcaac
      421 ttcgtcgtac ctgagtagag gtgtctgacc gtgcaagatt ctacttgtag cttggcttaa
      481 gggttgtagt atgtgtgcag ctttacctta gaatggtgag aagtaatctt tttaacggcg
      541 gtcaggagac cgcgatttat aaacgacgaa cttacaaact tcgccgaagg ggagtaggtt
      601 cgatgcagag cttgcagggt tttggaacaa ccggctctaa agctccttcc cgataaggaa
      661 tacttcatca atgcccttaa gcgcaagggc tatagggaat aaaaaaataa aggtggttta
      721 atccaaataa accaccttgt gcgttctacc gttgttgggt attacccaag cagtcccttc
      781 ttcatctttt cgcctattgc cctaagcatg tcatcggtca tggtttcgag gttccacttg
      841 gggctccagc cccattcctg gcgggcgcat gagtcgtcca tgtagttggg ccagctgtcg
      901 gcaatggcct tcttaaccgg gtcaacgcta tactccatgg tgaaatcggg aatccgtctt
      961 ctgattgccg agtaaacttg tgagggttca aagctcatgg cggttacgtt gaagctgttg
     1021 cggtgcttca gcttcgaggg gtcggcctcc ataa
//
LOCUS       DAOOQX010000770         1048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_204061_length_1048_cov_2.478348, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000770 DAOOQX010000000
VERSION     DAOOQX010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204061_length_1048_cov_2.478348"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLX53_10165"
     CDS             <1..561
                     /locus_tag="PLX53_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOY73489.1"
                     /translation="FTDIALVADEVQASFVVMGSSTIKEMDENKVSWVLKVITSCKVP
                     FITIQEPPVNKRYDEVVFPVDFTLENQEKHKWISYFSDFYLSRFHLVKPNTTDPELLA
                     KIDINMASARRFLDQKGAKSVEYTVPGLKPYEQEILSLAANIRADLIVLMTTPLDSEG
                     RFTVDPMEQYILANAGHIPVMCINPR"
     gene            573..>1048
                     /locus_tag="PLX53_10170"
     CDS             573..>1048
                     /locus_tag="PLX53_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOY73490.1"
                     /translation="MPQKLVFATNNVYKLKEVQAILGNAFQLLSLHDIGFIAEIPEDY
                     ETLEENAMQKARYVFSRTGYSCFADDTGLEVEALNGEPGVYSARYAGEDKKSTENIKK
                     LLMKLNGVENRNARFRTVIALTLSGKDYLFEGTVNGKIIDSPNGFDGFGYDPVFIP"
BASE COUNT          298 a          251 c          233 g          266 t
ORIGIN      
        1 ttcaccgata tagccctggt ggccgatgag gtacaagcca gctttgttgt catgggctca
       61 tccacaatta aagaaatgga tgaaaataaa gtttcatggg tgctcaaggt gattaccagc
      121 tgcaaggtgc ccttcattac cattcaggag cccccggtaa acaaacgcta cgacgaggtg
      181 gtttttcctg ttgattttac ccttgaaaac caggaaaagc acaagtggat atcctacttc
      241 agcgattttt acctgtcgag gtttcatctg gttaaaccca acactaccga tccggaactg
      301 ctggccaaga ttgacatcaa catggcttcg gcacgtagat tccttgacca aaagggcgct
      361 aaatctgttg aatatactgt tcccgggtta aaaccttacg agcaggagat tttgagcctt
      421 gccgccaaca ttagggccga tctgattgtt cttatgacta caccccttga cagcgaaggg
      481 cggtttacgg ttgatcccat ggagcagtac attcttgcca atgctggcca catcccggtt
      541 atgtgcatca atccacgcta ggtatgatta acattccaca aaaactggtt tttgccacca
      601 acaacgtcta taagctaaaa gaagttcagg ccattcttgg aaatgctttc cagctgctat
      661 ccctacatga tattggattt attgccgaaa tacccgaaga ctatgagacc ctggaggaaa
      721 atgccatgca aaaagcccgt tacgtatttt caagaacagg gtacagctgc tttgccgacg
      781 acacaggact tgaagtagag gcattgaatg gcgaacctgg cgtttacagc gctcgatatg
      841 ctggcgaaga caaaaaatca accgaaaaca ttaaaaaact tctgatgaaa ctcaacgggg
      901 ttgagaaccg taacgccaga ttccgtactg ttatcgccct gacactctcc ggaaaggact
      961 acctttttga gggaactgtc aacggaaaaa taattgactc ccccaatggc tttgacggtt
     1021 ttggatacga tccggttttc atccccga
//
LOCUS       DAOOQX010000771         1041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_206387_length_1041_cov_2.616633, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000771 DAOOQX010000000
VERSION     DAOOQX010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206387_length_1041_cov_2.616633"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..>1041)
                     /locus_tag="PLX53_10175"
     CDS             complement(167..>1041)
                     /locus_tag="PLX53_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73491.1"
                     /translation="KHLLFGEVAAELPTMKLAGIAGGFFRLTNTVQLAMIGRMYARDF
                     NPRYTNGFAEGSGVSNERGLYTGLSVLPAKGWKLTTYVDIFKFPWLRYQVNAPSEGRE
                     FMAQSDAVIAENFSLTIRYRGKEAPKNLSGSTAQVTPVVGQTTQSLRLQLGYLPLETV
                     RLKTTLEASAFSNDSLATEKGYLVAQDISLNPKRFPLSVNARFAVFDTPSWNSRIYSY
                     ESDMLYSFTVPAYYSKGTRLFVMVKYSLKNRLDVWLRYSQTYFSELDELGQGADLVEA
                     NTRSEIKAMVRFKF"
BASE COUNT          253 a          246 c          258 g          284 t
ORIGIN      
        1 aggtactttg gagataaaaa tattggtagc tgcatgaatt gaaatacttc agaaaggtat
       61 agggcaaaac aagcttcgcc gctacactaa aactacggtt tggaattaat aaggaatgga
      121 ataacaattt agcaatttta ttggttggtt atagcgtcaa gtagctctaa aatttaaacc
      181 tcaccatcgc cttaatctcg cttcgggtat tagcctctac caggtcggcg ccctgtccca
      241 gctcgtccag ctcgctgaag taggtctgtg agtaccgcag ccacacatcc aaccgatttt
      301 ttaagctgta cttgaccatt acaaaaagtc gggttccttt tgagtagtaa gctggcactg
      361 taaaagagta gagcatatcg ctttcgtagg agtaaatgcg actattccac gatggggtat
      421 cgaatacggc gaagcgtgca tttacactta aggggaaacg tttggggttt aagctgatgt
      481 cctgtgccac caggtagcct ttctcggtgg caaggctgtc gttgctgaat gctgatgcct
      541 caagtgtggt ttttagcctt accgtttcga ggggaaggta gccgagctgc aaccttaggc
      601 tctgcgtggt ttgtcccact acaggtgtaa cctgtgcagt tgagcccgac aggttctttg
      661 gtgcctcctt gcccctgtac cttatggtga gggaaaagtt ttctgctatt acagcgtccg
      721 attgagccat gaactcccta ccctccgatg gggcattcac ctggtatctc agccacggaa
      781 atttaaagat atcaacatag gttgttagct tccaaccctt tgcggggaga acggatagcc
      841 cggtgtataa ccctctttcg ttcgatacgc cgcttccctc ggcaaatcca ttggtgtatc
      901 gggggttgaa gtctcgggcg tacatcctgc caatcatggc tagctgaacg gtgttggtca
      961 gccggaaaaa accgcccgca atacctgcaa gcttcatcgt tggtagctct gctgcaacct
     1021 ctccaaacag caggtgttta c
//
LOCUS       DAOOQX010000772         1039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_207125_length_1039_cov_2.364837, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000772 DAOOQX010000000
VERSION     DAOOQX010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207125_length_1039_cov_2.364837"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1039
                     /locus_tag="PLX53_10180"
     CDS             <1..>1039
                     /locus_tag="PLX53_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOY73492.1"
                     /translation="PHEIANVAGVEGINFMLSSAIESPIKLFFGAPSCVPASPFDECF
                     QPFSPGIIKELIDRSDIFFLGEMMNFPGVIGGNAEVMEKINIAKSAGKPIDGHAPSLD
                     KNDLKKYISAGISTDHESFTLEEAIDKLSFGMKVIIREGSAAKNFDALHSLIPQFHDN
                     LMFCTDDCHPDDLIVGHINKLVKKAINAGYNIFDILQVSSVNPVMHYNLNVGLLQPGD
                     PADFIVVDGLSSLNIISTYINGDDVLKEPRPTTNVKIPTYTFPNSFDSNLLVKKLDTK
                     RVKVIDVVKDELITNWHVENSNTDLLEANPKEDLLKIAVVSRYKKNETSVGLIRGFGI
                     NKGAIAASIAHD"
BASE COUNT          310 a          207 c          220 g          302 t
ORIGIN      
        1 tcctcatgag attgccaatg ttgcaggggt cgagggcatt aattttatgc tgagtagcgc
       61 aatagagtca cccataaaac tattttttgg cgcaccgtcg tgcgtaccag cttcaccatt
      121 cgatgaatgt tttcagcctt ttagcccagg tataatcaag gagcttatcg atagaagcga
      181 tattttcttt ttgggtgaga tgatgaattt ccctggtgtt atcggtggta acgcagaggt
      241 tatggaaaaa atcaacattg ctaaaagcgc aggtaaaccc atcgatggac atgcgccctc
      301 gttggacaaa aacgacttaa aaaaatacat ttcagccgga atttctaccg atcatgagag
      361 ttttactctc gaagaggcaa ttgacaagct atcgtttgga atgaaggtaa ttatccgcga
      421 aggtagtgcc gctaaaaatt ttgacgccct gcattcgcta attcctcagt ttcatgataa
      481 ccttatgttt tgcaccgacg actgtcatcc ggacgatctt atagttggac atataaacaa
      541 gcttgtaaaa aaagccatca acgcggggta taatatcttc gacattctcc aggtttcttc
      601 tgttaatcct gtaatgcatt acaacttaaa tgtgggatta cttcagcccg gagatcctgc
      661 cgattttatt gtagtcgatg gtttaagtag ccttaacatt atttctacat acataaacgg
      721 tgatgacgtg cttaaggagc caaggccgac aacaaatgtc aaaattccaa cttacacttt
      781 tcctaattcg ttcgattcaa acctgctcgt taaaaagctg gacactaagc gtgttaaggt
      841 tattgatgta gttaaagatg agcttataac caactggcat gttgagaact ctaacaccga
      901 tctgctggaa gcaaacccaa aggaagatct cttgaagatt gctgttgtaa gccgttataa
      961 gaaaaatgaa acatctgttg gtctaatcag gggttttggt attaacaaag gagccattgc
     1021 agcaagcatt gcccatgat
//
LOCUS       DAOOQX010000773         1038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_207365_length_1038_cov_3.180061, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000773 DAOOQX010000000
VERSION     DAOOQX010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207365_length_1038_cov_3.180061"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1038
                     /locus_tag="PLX53_10185"
     CDS             <1..>1038
                     /locus_tag="PLX53_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOY73493.1"
                     /translation="FRRFNRNRIWESRVICLLSEKFNLQRVSEAEFLPAAVENAKENL
                     YPLVEWLNQHVNQLKTEGFAVAQLVRGMNFYLNGVVVAISTDSTTDWFDLYGKIKLDG
                     FEIPFVYLKRNILKNIREFLLPNGQVFIIPEVWFSRYKPLMLIGKEENEGIRLPKSLF
                     GLLADTNEVNSPAASELRHRLSNLKEEGIQLPQRLKARLRSYQLVGYAWLKLLNQNGI
                     NGCLADDMGLGKTIQALTLLQSINEEQNYATGMGKTSLVVVPTSLVANWISEAGRFTP
                     ELKVAAYMGQQRVKSASWLLNHHVVVTTYGLIRNDIDMFREVTFRYVVLDESQTVKNP
                     LTKGYRSVMQLR"
BASE COUNT          288 a          244 c          261 g          245 t
ORIGIN      
        1 tttcgccgct ttaaccgaaa tagaatatgg gaaagcagag ttatttgctt actttccgaa
       61 aagtttaacc ttcagcgggt tagcgaggcc gaatttttac cggcagcggt tgagaatgca
      121 aaggaaaatc tatacccctt ggtggagtgg ctcaaccaac atgttaacca gcttaaaacg
      181 gaaggttttg ccgttgccca actggttagg ggtatgaact tctacctcaa cggggttgta
      241 gtggccattt caaccgactc caccaccgac tggtttgacc tgtatggcaa aatcaagctc
      301 gatgggtttg aaattccatt tgtttacctg aagcggaaca tcctcaaaaa catcagggaa
      361 tttctgctgc ccaacgggca ggttttcatt ataccggagg tatggttcag ccgttacaaa
      421 cccttaatgc tgataggcaa ggaggaaaat gaaggcataa ggttgccaaa atcgcttttt
      481 ggcctgctag ccgacaccaa tgaagtcaac tcccccgcag cttcggagct caggcacagg
      541 ctttccaacc tgaaggaaga aggtatccag ctaccccaac ggttaaaggc ccggctacga
      601 agctaccagc ttgtagggta tgcatggctt aagctgctaa accaaaatgg catcaacggt
      661 tgtctggccg atgacatggg actgggcaaa accatacaag ccttaacgtt gctacaatca
      721 atcaatgagg agcaaaacta tgccacagga atgggtaaaa cctcgttggt tgttgtgcca
      781 acatcactgg tggcaaattg gattagcgag gcgggcaggt ttacccccga gctgaaagta
      841 gccgcctaca tggggcagca aagggtaaaa tcggccagct ggttgctgaa ccatcacgtt
      901 gtggttacca cgtacggcct catccgtaac gatattgaca tgttccgtga ggtaaccttt
      961 cgatatgttg ttctggatga gagccaaacc gtaaaaaatc cgttgaccaa aggctaccgc
     1021 tccgtaatgc agctgagg
//
LOCUS       DAOOQX010000774         1033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_209237_length_1033_cov_2.125767, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000774 DAOOQX010000000
VERSION     DAOOQX010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209237_length_1033_cov_2.125767"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PLX53_10190"
     CDS             <1..506
                     /locus_tag="PLX53_10190"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOY73494.1"
                     /translation="ALVSLIRKVVGIDKALTRLQNLCARQEKCVWDIMQKLKLWGINN
                     EEAQKIVSMLVDEGFVDDRRYAQMFVREKSQLNKWGSIKIAAALRVKKINESIIKEAL
                     LELQPQFDRATLENIITTKANQVKAKSPSDLRAKLVRFGLSRGFELEHVVKTVDMVMK
                     SNMTDEA"
     gene            698..>1033
                     /locus_tag="PLX53_10195"
     CDS             698..>1033
                     /locus_tag="PLX53_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HOY73495.1"
                     /translation="MLKIFTRFDSKNHRPVMGAMEIFKFIGPGLLVTVGFIDPGNWAS
                     NLAAGADYGYKLLWMVSLSTIMLIVLQHNVAHLGIATGLCLSEATTKYLKPGVSRFIL
                     GTGMLASVST"
BASE COUNT          323 a          197 c          262 g          251 t
ORIGIN      
        1 tggctttggt ttcattgatt cgcaaagttg ttggtattga taaagcttta acacggctcc
       61 aaaacctatg cgcaaggcag gaaaagtgcg tgtgggatat catgcagaaa cttaaactat
      121 gggggattaa caacgaggag gctcaaaaga ttgtctccat gttggttgat gagggatttg
      181 ttgatgacag aaggtatgca caaatgtttg taagggaaaa gagccagcta aacaagtggg
      241 gcagcattaa aattgcagca gctctgcggg taaaaaaaat taacgaatct ataattaagg
      301 aggcgctgct cgagcttcag ccccaattcg accgggcaac tctggaaaat attattacca
      361 caaaggccaa ccaggttaag gctaaaagtc cgtcggactt acgggcaaaa cttgtccgat
      421 ttgggttgtc gaggggtttt gaactggagc atgtggtaaa aacggtggac atggttatga
      481 aaagcaatat gacagatgag gcctagctct actccactat gaataaacgg aaaagtcagc
      541 attggagcac caagccaaag tatatcatat tgcgcaacag cagaaaaaaa ttgagttagc
      601 gcaaaggtaa aaagtagaac tttttcacct aaaaaagggt tactaatatt aaggtggtaa
      661 gcaacaaaac aggtaacgga taaatagcgg tttaaagatg ctaaagattt ttacaaggtt
      721 tgattccaaa aaccatcgtc ccgtgatggg agcgatggaa atattcaagt ttattggacc
      781 cggactgctg gtaactgtgg gatttattga tcccggtaac tgggcttcca acctcgcggc
      841 gggagccgac tatggctata agctgctatg gatggtgagc ctgagcacca ttatgctcat
      901 cgtccttcag cataacgtgg cacatttggg aattgccacc gggttgtgcc tgtccgaagc
      961 aaccacaaag tacctaaaac ctggcgtttc aaggtttatc ctgggcaccg gaatgctggc
     1021 atcagtctca acc
//
LOCUS       DAOOQX010000775         1032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_209489_length_1032_cov_2.866940, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000775 DAOOQX010000000
VERSION     DAOOQX010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209489_length_1032_cov_2.866940"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1032
                     /locus_tag="PLX53_10200"
     CDS             <1..>1032
                     /locus_tag="PLX53_10200"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOY73496.1"
                     /translation="DIYPIYEDLQGRIWVGTKDGLMLFDRERMLFKRYSSRISYPYSI
                     YGNRVQAIYEDSRGNFWVGTDKGLNLFSFKLQRFYPVGAGIDENDAVRFIFEDSRSTL
                     LLGMESGVARLHSSTEKVEQLQNFYSKSSKILPIGISAILKDKSGITWVASRTGLIKI
                     NPFSQRFKGYSRDNLGNPLFSNNIIASVFEDSSGKLWVGTWGRGLFIFNPKTGENLRY
                     CKSSPIYRLPNDFAHTVFQTRKGEILVGTRNGVFVYDQRSRQFSNFFASRHVSVGNIF
                     VDNRVYSIDEDSAGNLWFATAIGLHCLSGNRLHSYYSRRGDSIFLPSNLIFDVLAGKQ
                     GEVWVATANG"
BASE COUNT          284 a          190 c          249 g          309 t
ORIGIN      
        1 gatatatacc ccatttacga agacctgcaa ggaagaattt gggtgggtac caaggatggg
       61 ttgatgctgt tcgataggga aagaatgctt tttaaacgtt acagtagccg aatatcctac
      121 ccttactcaa tatatgggaa cagagttcaa gccatttatg aggatagtag aggtaatttt
      181 tgggttggta ccgataaggg gcttaaccta ttcagcttta agctgcagcg attctatccg
      241 gttggggcag gcatagatga aaacgatgcc gtcaggttca tttttgagga tagccgctca
      301 acccttttac ttggaatgga aagtggggtt gcgaggttac attcttctac agaaaaagtt
      361 gagcagctac aaaattttta tagcaaaagc agcaaaattt tacctattgg catctctgct
      421 attctaaagg ataagtcggg aattacctgg gttgcctcac gtacaggttt gataaaaatc
      481 aaccccttta gccaacggtt taagggctac agtagggaca acttgggcaa tccgcttttc
      541 tccaataaca ttattgcatc tgtttttgag gattcctccg gaaaactctg ggtaggaaca
      601 tggggtagag gtctgttcat tttcaatccg aaaactggag aaaacctgag atactgcaaa
      661 agttcaccaa tctaccgctt acccaatgat tttgctcata cagtatttca aaccagaaag
      721 ggtgaaattc ttgtgggaac ccggaatggt gtctttgtgt atgaccagag aagcaggcag
      781 tttagcaatt tttttgccag taggcatgtt agcgttggta atattttcgt cgataatcgc
      841 gtgtattcca ttgacgagga ttccgctgga aacctttggt ttgctacggc cattggtttg
      901 cattgcttaa gtggtaatcg cctgcatagc tactattccc gtaggggtga ttccatattt
      961 ttaccttcga atttaatttt tgatgtgcta gctggaaagc aaggagaagt ttgggtggct
     1021 accgcaaatg gt
//
LOCUS       DAOOQX010000776         1030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_210160_length_1030_cov_3.085128, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000776 DAOOQX010000000
VERSION     DAOOQX010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210160_length_1030_cov_3.085128"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            801..929
                     /locus_tag="PLX53_10205"
     CDS             801..929
                     /locus_tag="PLX53_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73497.1"
                     /translation="MVEFTRDMQLLPALPASALLLHNSCRVANPSLPANNAYHVYL"
BASE COUNT          261 a          243 c          226 g          300 t
ORIGIN      
        1 aaggctattg gaaataacca tgtagcccag ctctaccttc accccctgct tcgcctccac
       61 ggtatcgcca ctctggcaaa acgcaggaga agccgttaga atgagcagca gggctatgtg
      121 gcaacgtttc gtgatgctat tcataaattc attttttggg gcttatactc attagtagct
      181 tgaaactatt cgaaaaacct agcgatagac cctgaaggtg gaaaaaattg taacgagttt
      241 ttttctgttc ttcacaatga aataaactaa taaaggcatt cattagaaaa tattttgata
      301 tatgagggga agcgccttta ccctttttcc gcaaccgaca ggccatctcc cgtagcccaa
      361 aggttgatta cttttttagt cagctcaagc tcatttccta taatttttaa ccggcctaac
      421 ctgccggcaa attttttatg caattgtacg gcatattcca gctcgtgtca agttttttct
      481 attgttaaac ggcacgggtg aaagctgtgg gggaaatttt ggctgctgat aacggccatt
      541 actcctgctc cgccatcctg aagaataagc tatcaacatg ttacaaacag gctatcaaca
      601 cgctgctaac cgctatagag gtctgcgtgt taataatgat gataacggct tttctattcc
      661 cagtggcacc tgcagcgttc caatgcgatt tgcaacaagt cgggagtcgg aaattcgggc
      721 gaagctaggg gcttgctgca ggacagtgtt gagcttggtc gggcattaaa gtagagcttg
      781 ggttacccgg ggctttgtgt atggttgaat ttacaaggga tatgcagctt ttaccagctt
      841 tacccgcttc ggcattgctg ctgcacaact cctgtcgtgt ggcaaaccca tccctgcctg
      901 ccaacaacgc ttaccacgtc tatctttaag tatataccgt atagtccagc cggattattc
      961 ttttgctaat tcctgcccac tactttcttt ctctatacca ccacacgagg ggacttttgc
     1021 agtagcgggg
//
LOCUS       DAOOQX010000777         1030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_210223_length_1030_cov_2.552821, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000777 DAOOQX010000000
VERSION     DAOOQX010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210223_length_1030_cov_2.552821"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLX53_10210"
     CDS             complement(<1..866)
                     /locus_tag="PLX53_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family peptidase"
                     /protein_id="HOY73498.1"
                     /translation="MIVILVAASCKNNSSEVKNPLLAEFDTPFQTPPFDKIRHEHYAP
                     AIDSAIAEAKAEVEAITSNPAKSTFENTIEALDRSGRRLNLVSNILFNLNESETDSTL
                     QGIARDAAIKLTEFSNDVNLNPELFKRVKQVWEMRDSLKLTTEQQTLLSNTYKGFIRN
                     GANLSDSDKEKFRAISKELADLSLRFNENLLAETNSYQLHITNESDLAGLPASLKDAA
                     AYTAQQKGKVGWIITLDYPMYGPFMKYSENRELRKQLYMAYGSRCFKGNEYDNQAIAK
                     KIANLRLEIANL"
BASE COUNT          256 a          228 c          237 g          309 t
ORIGIN      
        1 aacaggttgg caatttctaa ccgtaggttg gcaattttct tagcaatagc ctggttgtcg
       61 tactcatttc ccttaaagca acggctacca taagccatat agagttgttt gcgcagctcc
      121 ctattttcgg aatacttcat gaatggacca tacattgggt aatcaagcgt aattatccat
      181 ccaactttcc ccttttgctg agctgtgtag gcagcggcat cctttaaact agccggaagt
      241 cctgccaagt cgctttcgtt ggtaatatga agctggtagc tgtttgtttc ggcaagcagg
      301 ttttcgttaa aacggagcga taagtcggcc aactccttac ttatggcacg aaacttctcc
      361 ttgtcgctat cggaaaggtt tgccccgttt ctgataaatc ccttgtaggt attgctgagc
      421 agcgtttgct gttcggtggt aagcttcagg gagtcacgca tttcccacac ctgcttaacc
      481 cttttaaaaa gttccgggtt gaggttgaca tcgttgctaa actcggtgag cttgatggcc
      541 gcatcacgag caataccctg cagggtcgaa tccgtttcgg actcattgag gttgaacaga
      601 atgttcgaca ccaggttcaa acgcctaccg cttcggtcga gcgcttcaat ggtgttttcg
      661 aatgtacttt tggcaggatt actagtaata gcctcaacct cagcctttgc ctcggcaatg
      721 gccgaatcaa ttgccggtgc ataatgctca tgccttatct tgtcgaaggg cggagtttgg
      781 aatggtgtgt caaattcagc cagcaggggg tttttcacct cgctgctatt atttttgcag
      841 gatgctgcaa ctagtataac tatcattgtt ccagtaagtt tgccgtagat attcatttta
      901 aaaacgattt aaaagtgttg gccgcaaagg tagaaaatta tcgatagaac atggagaatt
      961 acaaaaatac cgattttaaa actcatacaa agggtaaatg ctgcctgtgg ctcatccctg
     1021 gataattctg
//
LOCUS       DAOOQX010000778         1030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_210297_length_1030_cov_2.121026, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000778 DAOOQX010000000
VERSION     DAOOQX010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210297_length_1030_cov_2.121026"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLX53_10215"
     CDS             <1..502
                     /locus_tag="PLX53_10215"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOY73499.1"
                     /translation="SAFVAPGGYIYLYTGILMALDNEAQLAGLLAHQLAHIDCRHITK
                     MLLRHYDLSTFESIAAGLEQAALDSIIHGLVNGSSTLKYSCSNEHEADSKAMEYLFDT
                     GYDPYELINMYRKLLTCSGVAQFFAIHPWDDGRIENLEAAWKNLGARHGEKYTERYLD
                     FKKSLP"
     gene            complement(679..831)
                     /locus_tag="PLX53_10220"
     CDS             complement(679..831)
                     /locus_tag="PLX53_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73500.1"
                     /translation="MSIKGVFKKLSKNEKILLVIIILLLIMVATQWEKIWNEAIRAFG
                     RYFGSK"
     gene            complement(843..>1030)
                     /locus_tag="PLX53_10225"
     CDS             complement(843..>1030)
                     /locus_tag="PLX53_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOY73501.1"
                     /translation="GMIKGARGQEPVNENLFADIISRVSVLVGVAPEIFEMDINPLLG
                     TKDKVVAVDARIRIEKK"
BASE COUNT          284 a          238 c          211 g          297 t
ORIGIN      
        1 cagtgcattt gttgctccgg gaggttacat ctacctctac acgggcatat taatggctct
       61 tgataacgaa gctcagctgg cagggctact ggctcaccag ctggcccaca ttgattgccg
      121 gcatataacc aagatgcttt taaggcatta tgatttatca acgtttgaaa gtattgctgc
      181 tggtctcgaa caagctgcac tcgatagtat aatccatggg ttggtcaacg ggagctctac
      241 cctaaaatac agctgcagca acgaacatga agccgatagt aaggccatgg aatacctgtt
      301 cgatacaggc tatgatccct atgagcttat caacatgtac aggaagctac ttacatgctc
      361 tggggttgct caattctttg ctattcaccc atgggatgat gggaggatag aaaacttgga
      421 agctgcgtgg aaaaatctgg gtgccaggca tggcgaaaaa tatacagaac gctacctgga
      481 ttttaaaaaa tctttacctt gatgattcct gtattttagc cccttctaaa ggggcaaatc
      541 aaactcattt ggtatagact ttaaaaccca gcttgtattg aaaaaagggg tagtctcacc
      601 actcacgatt ttcccgatga aagtaaatgt gaaaatgccc ccacaactag gctttttcat
      661 tccttaaaag aatacggttt acttgctgcc aaagtacctg ccaaacgctc tgatagcctc
      721 gttccagatt ttttcccatt gggtagctac cataatcagc aaaaggataa tgatgacgag
      781 tagtattttt tcgtttttgg aaagtttttt aaaaacaccc tttatgctca ttaccagcta
      841 ggctatttct tttctattct gattcgcgca tcaacagcta ccactttgtc ctttgttccg
      901 agcaaggggt tgatgtccat ttcaaatatt tcgggcgcca cacccaccaa caccgaaacc
      961 cggctgatga tgtcggcaaa caagttttcg tttacaggct cttggcccct agccccttta
     1021 atcattccat
//
LOCUS       DAOOQX010000779         1027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_211233_length_1027_cov_3.382716, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000779 DAOOQX010000000
VERSION     DAOOQX010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211233_length_1027_cov_3.382716"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLX53_10230"
     CDS             <1..645
                     /locus_tag="PLX53_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOY73502.1"
                     /translation="KLFAFLSKPNLKTLQKDPAFIAAQSINAKAMELYGLMEPFNQKL
                     QKGQRLFIAGIMEMNKGKALYPDANFTMRLTYGEIKDYYPMDAVHYDYQTTLDGVMEK
                     EDPDNWEFVVPQRLKDLYQAKDYGRYGVNGKMPIAFISTNDITGGNSGSPVMNGNGEL
                     IGLAFDGNWEAMSGDIVFENNLQRTISVDIRYVLFIIDKYAGATRLVDEMQIVE"
BASE COUNT          324 a          221 c          206 g          276 t
ORIGIN      
        1 aagctatttg ccttcctgtc gaaaccgaac ctgaaaaccc ttcaaaaaga tcccgcattc
       61 attgccgccc aatcaattaa cgcaaaggca atggagcttt acgggttaat ggaacccttc
      121 aaccaaaagc tgcaaaaagg gcagcgactc ttcatagctg gaattatgga gatgaataag
      181 ggcaaggcgc tctaccccga tgccaacttt accatgaggc ttacctacgg cgaaataaag
      241 gattactacc ccatggatgc cgttcattac gactaccaaa ctactctcga tggcgtaatg
      301 gaaaaggagg atccggacaa ttgggaattt gtagtgccgc aacgtcttaa agacctatac
      361 caggctaagg attatggccg ctacggcgta aatggcaaaa tgcctatcgc cttcatttca
      421 accaacgaca tcaccggtgg aaactccgga agccctgtaa tgaacggtaa tggcgaactc
      481 ataggtttgg cattcgacgg taactgggag gccatgagcg gtgatatcgt attcgaaaac
      541 aacctgcagc gaacaatttc ggttgatatc cgctacgtgc tattcataat tgataagtat
      601 gccggtgcaa ccaggctggt ggacgagatg caaattgtgg agtagcacag aatttcctat
      661 cggttttaac ccataataat agcaaagggg atgccagtat ttggcatccc cttttcatca
      721 tcaaaccgcc atataaccat tcggcaaaac catttaatta caaaattaat ccattcgtca
      781 aaaatacttt tcacaaatac aattttaaaa tgaatattgg ttgacaaatt agggtaggtt
      841 acaccgtttt aaacttgatg ttttgcatgc aacctaactc cttagatttc actagctact
      901 ttttttaaca gaagtttttt agggtaataa ttgcaatttt attgttctat ttcataattt
      961 agtaaaaatt ttgtagcact ataaggtgaa aaatagcgaa gtactcatca gccataatgg
     1021 gccactt
//
LOCUS       DAOOQX010000780         1026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_211713_length_1026_cov_2.342945, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000780 DAOOQX010000000
VERSION     DAOOQX010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211713_length_1026_cov_2.342945"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1026
                     /locus_tag="PLX53_10235"
     CDS             <1..>1026
                     /locus_tag="PLX53_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOY73503.1"
                     /translation="WTFAPMVDIARDPRWGRVMEGAGEDPFFGSAVARARVKGFQGSK
                     LSDTSSIAACAKHFVAYGAAQAGRDYNTVDISERTLYEVYLPPFKSALDAGVVTFMSS
                     FNELNGIPVTGNKAMVNDLLKEKWGFNGFVDSDWGSIREMIYHGYAANEYEAGYLAMS
                     SGVDMDLEGRIYLAQLKKLVEDGRITVEQLDEAVRRILRVKFELGLFDNPYKGCDSIR
                     EKELLLNPRHLEIAREVTRKSLVLLKNENVLPLSRNIHSIAVVGPLADNQDELIGTWS
                     GRGEGKHVVSVLSGIREKLPNGNIYYAKGCEIVGENKAGFDEAINAARKADAVVVVVG
                     ESDMMSG"
BASE COUNT          259 a          194 c          284 g          289 t
ORIGIN      
        1 attggacatt tgcaccaatg gttgatattg cccgtgatcc tcgttggggt agggtaatgg
       61 aaggtgctgg cgaagacccc ttcttcggtt ctgccgttgc tagagcccgg gttaagggat
      121 ttcagggtag taagctatcc gacaccagct ctattgctgc ctgtgccaaa cattttgttg
      181 cctatggcgc tgcccaggct gggagggatt acaacacggt agatatttcc gagcgaacac
      241 tctacgaggt ttacctgcct ccttttaaat cagcgcttga tgcaggtgtt gtcaccttca
      301 tgtcgtcatt caacgagctt aatggcattc ctgttacagg aaataaggcc atggtaaacg
      361 atttgcttaa ggaaaaatgg ggctttaatg gctttgtaga ttctgattgg ggctctattc
      421 gtgagatgat ttaccacggg tatgctgcca atgagtatga agccggatac ctggccatga
      481 gctcaggagt ggatatggat ttggaaggtc gtatctattt agcccagctt aaaaagttgg
      541 ttgaggatgg tagaataacc gttgaacagc ttgacgaagc tgttcgcaga atccttagag
      601 tgaaatttga gctaggtttg ttcgataatc cctataaagg ttgcgatagc attcgcgaaa
      661 aagagctttt actaaacccc aggcatttgg agattgctcg cgaggtaacc cgtaaatcgc
      721 tcgtgttgct taagaatgag aacgttcttc cgttaagtag gaatattcac tctatagccg
      781 tagttggccc tctagccgac aatcaggacg agctaattgg aacatggtca ggtagaggtg
      841 agggcaaaca tgttgtttct gtgctatcgg gaattcgtga aaagctgcca aatggaaata
      901 tttactacgc taagggctgc gaaattgttg gtgaaaataa ggctggattt gatgaggcca
      961 ttaatgctgc ccgaaaggct gatgcggtag tggttgtggt aggtgaatcg gacatgatgt
     1021 ctggtg
//
LOCUS       DAOOQX010000781         1023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_212875_length_1023_cov_1.836777, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000781 DAOOQX010000000
VERSION     DAOOQX010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212875_length_1023_cov_1.836777"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLX53_10240"
     CDS             <1..483
                     /locus_tag="PLX53_10240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOY73504.1"
                     /translation="PGDSHTLEVIAPPTIDGEPGFVPFAILGDSLLTTEILNFQTRNR
                     YEIIIKATDKAGHSLEKSFFINVIKNTGVSDLTLSGEMIITPTNDGNLKVWLNNSAIG
                     RATMNLYDINGKLIFSTTISKELQVLDTTIPLGNTLIKGLYVAVLNFGNEKISYKFVK
                     "
     gene            563..>1023
                     /locus_tag="PLX53_10245"
     CDS             563..>1023
                     /locus_tag="PLX53_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73505.1"
                     /translation="MGMALVGISQECTTMWPYIYPEFKEGTLLMVDGSKLTAQFNVHV
                     QEGRLHYLVKGIIKEARSGDILLANIGDDIYMNVDGSVMKVVGSEERGFVATLILADF
                     DKLLNSTQGAYGASLNSSAKMKLSSIEVGGKTIVDHMLLKQRKEEGETLPL"
BASE COUNT          315 a          164 c          226 g          318 t
ORIGIN      
        1 cctggcgatt ctcataccct ggaggtaatt gcccctccta ctattgatgg cgagcctggg
       61 tttgtcccct ttgctatttt aggagatagc ttattgacaa ctgaaatttt aaattttcaa
      121 acgagaaacc gctacgaaat tattattaag gctaccgaca aggctggtca ttcgttggaa
      181 aaatcatttt ttattaacgt gattaaaaat acaggtgtta gcgacctaac tttaagtggc
      241 gaaatgatta ttacacctac aaatgatggc aacttaaaag tgtggcttaa caactcagct
      301 ataggtaggg caaccatgaa tttatacgac attaacggta aactcatatt ttctactacg
      361 attagtaagg aattacaagt gctggatacc accattccac taggtaacac tctaataaaa
      421 ggactttatg ttgcagtttt gaattttggt aatgaaaaaa tttcctataa atttgtaaag
      481 taggctaagg gtcaaggtat gaaatttaaa attttaaact cgttacagca tggttaagcg
      541 tattgtacaa atattttttt tgatgggtat ggcattggta ggtatttcgc aggaatgtac
      601 aaccatgtgg ccatacatct atcctgaatt taaagaggga actttactta tggttgatgg
      661 tagtaagcta acagcccagt tcaacgtgca cgttcaggag ggtcgtttac actatctggt
      721 taaagggatt ataaaagaag ctaggagcgg ggatattttg ctggccaata ttggagatga
      781 tatttacatg aatgttgacg gcagtgttat gaaggtggtt gggagcgagg aacggggatt
      841 tgtggcaacc ttaatacttg ctgattttga taagctttta aattctaccc agggtgcata
      901 tggtgcttcc ttaaactctt ctgctaaaat gaagctttct tccatagagg tgggcggtaa
      961 aactattgtt gatcacatgc tgctaaagca gagaaaagag gaaggtgaaa ccttgccatt
     1021 aat
//
LOCUS       DAOOQX010000782         1022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_213066_length_1022_cov_2.929679, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000782 DAOOQX010000000
VERSION     DAOOQX010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213066_length_1022_cov_2.929679"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..979
                     /locus_tag="PLX53_10250"
     CDS             263..979
                     /locus_tag="PLX53_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOY73506.1"
                     /translation="MGRAFEYRKARKLKRWGNMAIVFTRLGKEITMVAKSGGADPDTN
                     PKLRLLIQNAKSENMPKDNIERAIRRATDKDTKEYKEVVYEGYGPYGVAILVETATDN
                     PTRTVANIRSYFNKHGGSLGTSGMLDFMFEHKCSFRIDGKDSISLDELELELIDYGVE
                     EVFVEDDSILIFADFAQFGPVQRYLEGKGLHILNFQFERIPTDTKKLTPEQQEEVEKL
                     VDKIEEDEDVTHVFHNMEIA"
BASE COUNT          347 a          185 c          225 g          265 t
ORIGIN      
        1 ataattcaaa aatttttggc taaaattagc atttcaatca attctcatag gttaatattc
       61 cttaaaattt aaaaataagc atttttgttc aaaaataatt gaacagccaa tcccgaaatc
      121 aggatgaaat atcttggaaa tcgggttaga gaaaaaatta aaaattaaga aaatattttt
      181 tgcctaaaag tggagaattt tcatcatgta aactaaaaaa tgtaggtttg caaacttttt
      241 gatagaaaac aaatagcaag taatgggaag agcatttgag taccgcaaag cacgcaagct
      301 aaaacggtgg ggcaacatgg ccattgtatt tacccggtta ggaaaggaga taacaatggt
      361 ggcaaaatcg ggcggcgcag atcctgacac caaccctaaa ctccggttac tgattcagaa
      421 cgccaagtcg gaaaacatgc ccaaggataa tattgaaagg gccattaggc gtgcaaccga
      481 taaggatacc aaagaataca aggaggtcgt ctatgagggc tacggacctt atggggttgc
      541 catcctagtt gagaccgcca cagacaatcc tacacgaacg gtagcgaata tccgaagcta
      601 cttcaacaag catggaggct cgcttggaac ttcaggtatg cttgacttca tgtttgagca
      661 taaatgctca ttcagaatag atggtaaaga cagcatttcg ctcgatgagc tggagctgga
      721 gcttattgac tacggggttg aggaggtgtt tgttgaggac gatagtatcc ttatatttgc
      781 cgactttgca cagtttgggc cggttcaacg ttacctggag ggaaaagggc tacatatcct
      841 aaactttcag tttgaacgca ttccaacaga taccaagaag ctaactcccg aacaacaaga
      901 agaagttgag aaactggtgg ataaaattga ggaagatgaa gacgttaccc atgtttttca
      961 taacatggaa attgcttgac ctgccctatt tttctgaaaa attctacagc tgcatttcgt
     1021 at
//
LOCUS       DAOOQX010000783         1021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_213525_length_1021_cov_2.339545, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000783 DAOOQX010000000
VERSION     DAOOQX010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213525_length_1021_cov_2.339545"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="PLX53_10255"
     CDS             complement(<1..121)
                     /locus_tag="PLX53_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134999.1"
                     /note="Part of the NQR complex which catalyzes the
                     reduction of ubiquinone-1 to ubiquinol by two successive
                     reactions, coupled with the transport of Na(+) ions from
                     the cytoplasm to the periplasm; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOY73507.1"
                     /translation="MSDVKEKEPWFSAKNIELLTGPLNSKNPITVLVLGICSAL"
     gene            complement(114..851)
                     /gene="nqrC"
                     /locus_tag="PLX53_10260"
     CDS             complement(114..851)
                     /gene="nqrC"
                     /locus_tag="PLX53_10260"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOY73508.1"
                     /translation="MDKNSNLYTILYASGLVVLVAVLLALAATLLKPAQQRNVETEKK
                     IDILKSIGKADGVEKAANKHKFVASNFERYIVEQLVVNSLGERIQGVDAFNVDLKVEL
                     SKPLENRYLPVFVAKLDSGSTKFIFPLRGKGLWGPIWGYISLNDDFNTIYGATFSHQG
                     ETPGLGAEIATPQFQKQFDGKSIFDNQNQLVSIKVVKGGADKSDPHGVDAVSGGTITS
                     KGLEAMLYDCLSIYMPFINNQKQQSHE"
     gene            complement(890..>1021)
                     /locus_tag="PLX53_10265"
     CDS             complement(890..>1021)
                     /locus_tag="PLX53_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOY73509.1"
                     /translation="PAYPEGMMLSILLMNAFAPLIDYYVIQGNIRRRLKRTNRVAKS"
BASE COUNT          274 a          247 c          212 g          288 t
ORIGIN      
        1 ccagagcgga gcatataccc agcaccagca ctgttatggg gttcttggaa tttaacggac
       61 ctgttaggag ctcaatattt ttagccgaaa accagggttc tttttccttc acatcactca
      121 tgactctgct gtttttggtt gttaataaat ggcatataga tgctcaggca atcgtagagc
      181 attgcctcaa gccctttgct tgtaattgtg ccacccgaaa cggcgtcaac tccatgggga
      241 tcgctcttgt ctgcaccacc ctttaccacc ttaatggaga caagctgatt ttggttgtca
      301 aaaattgatt tcccgtcaaa ctgtttttgg aactgtggcg ttgcaatttc ggcaccaagc
      361 ccgggtgttt cgccctgatg cgagaatgta gccccgtaaa tagtgttaaa atcatcgttc
      421 agggagatgt aaccccagat tggcccccac agccctttgc ccctaagcgg aaagataaac
      481 ttggtggaac cgctatcaag ttttgccaca aaaacaggca aatatctatt ttctaatggc
      541 ttagataatt cgacttttaa atcaacgtta aaggcatcaa ctccctgaat tctctcaccc
      601 agcgagttca caacaagctg ctcaacaatg tagcgttcaa aattactggc tacaaactta
      661 tgcttgttgg ctgccttctc aaccccatcg gccttgccaa tcgacttcaa aatatcgatc
      721 ttcttttcgg tttcgacatt ccgttgctgt gcaggtttca gaagagtagc tgctaacgcc
      781 aggagtactg caaccagcac cacaagcccg gaagcgtaaa gtatggtgta caggttgctg
      841 ttcttgtcca tactcttcaa ttttccaaaa atgctcatag tgaaaaaagt tatgatttag
      901 caaccctatt agttcttttc agtcgtcggc gaatattccc ctgaattacg tagtagtcaa
      961 tcagaggggc aaaggcattc ataagcagaa tggaaagcat catcccttca ggataggcag
     1021 g
//
LOCUS       DAOOQX010000784         1020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_213785_length_1020_cov_3.185492, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000784 DAOOQX010000000
VERSION     DAOOQX010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213785_length_1020_cov_3.185492"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1020
                     /locus_tag="PLX53_10270"
     CDS             <1..>1020
                     /locus_tag="PLX53_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73510.1"
                     /translation="FSYNIATGEVDKVTRVNGLSEVGISALSYFPSLQILAIGYESGN
                     IDLLVQNSVVNLPFIKEKPLSGSKTINRFTLFNNLIYTSTDFGLVVVDPAKREIKETY
                     YIGNNGTGIRVSSMEVFGDRFWAATSNGLKSALCSDPLLVSFEHWTTETSFTNPTAEC
                     AGVAAKDSYLFALERQENANDILWRFDGSSWVEVDRPLPATLHLSVGNNRILVTSANG
                     IQTYQFPGIRDEFLSPASLFINPQAAISVDADKLAIADKFNGLMFGSSQALRSIYPNG
                     PANNNHLAIDVSSNRVVVASGGYDATFTNLWHPFMVHDFTHEEWSSFADYSYHDAVAV
                     RVNPG"
BASE COUNT          269 a          234 c          238 g          279 t
ORIGIN      
        1 tttctcatac aatatagcca ccggtgaggt tgataaagtt acgagggtga atggcctttc
       61 ggaagttgga atctcggcac ttagctattt cccttcattg caaatacttg ccattggcta
      121 cgaaagtgga aacatagatt tgctggttca gaacagcgta gttaaccttc cctttataaa
      181 ggaaaaaccc ctttcgggta gtaaaaccat taaccggttt acccttttta acaacctgat
      241 ttacacttca accgattttg gactagtggt ggttgatcca gctaaaaggg aaataaagga
      301 aacctactat atcggaaaca atggaaccgg catccgggtg agcagcatgg aggtatttgg
      361 ggatagattc tgggctgcaa cttccaatgg cctgaaaagt gccctgtgct ccgaccctct
      421 tctggtaagc tttgagcatt ggactactga aacatcgttt accaacccta ctgccgaatg
      481 cgctggagtt gctgctaagg atagctacct ttttgctctg gaaagacagg agaacgccaa
      541 cgacattctc tggcgattcg atggaagctc atgggttgaa gttgaccggc ctttgcctgc
      601 cacccttcat cttagcgtag gcaataatag gattctggta acctccgcta atgggattca
      661 aacctaccaa tttccgggga ttagagatga atttctttca cccgcatcac tgtttatcaa
      721 cccacaagct gcaatttcgg tagatgcaga caagctggcc atagccgata agtttaacgg
      781 tcttatgttt ggtagttctc aggcgctaag gtctatctac ccaaacggtc ctgcaaacaa
      841 caaccacctt gccatagatg ttagcagcaa tcgtgtggtt gtggcctcgg gaggctacga
      901 tgcaaccttc accaacttgt ggcatccctt catggttcat gatttcacgc atgaggaatg
      961 gagcagtttt gctgattata gctatcatga tgccgttgcg gtaagggtta atccaggcaa
//
LOCUS       DAOOQX010000785         1020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_213931_length_1020_cov_2.172021, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000785 DAOOQX010000000
VERSION     DAOOQX010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213931_length_1020_cov_2.172021"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLX53_10275"
     CDS             complement(<1..270)
                     /locus_tag="PLX53_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73511.1"
                     /translation="MKKIFLAALLGLPLMAAAQNQEAGKQEKVKTGWNLGGLPVIAYD
                     TDLGLEYGGLVNLFYYGDGSLYPQYLHNIYVEVSRFTKGSGINRLF"
     gene            complement(544..>1020)
                     /locus_tag="PLX53_10280"
     CDS             complement(544..>1020)
                     /locus_tag="PLX53_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73512.1"
                     /translation="HLIKLRDLQNRKPTNAPGFVNFIVWTFQGKGTVLEKEGIVPHVT
                     DEEYIRTIAISRIMLNNIKNIQASWLTTGIEVAQVCLHAGANDLGSIMIEENVVSSAG
                     AKYRTDAYGIQKAIADAGFEPQLRNQKYEFVDLPKCEYSRIYTLNELKNRQVNQAG"
BASE COUNT          238 a          228 c          250 g          304 t
ORIGIN      
        1 aaacaacctg tttatcccgc ttcccttggt gaaacgcgac acttctacat aaatgttgtg
       61 caggtactgt gggtaaagcg agccatcgcc atagtagaac aggttaacta gccctccgta
      121 ctccaggccc aaatcggtat cgtaagcgat tacgggcaac cctccaaggt tccacccggt
      181 ttttactttt tcctgcttac cagcttcctg gttttgtgct gccgccataa gcggtaggcc
      241 taacagggcg gccagaaaaa tttttttcat cctgtgaggt gtttgttact tcaccaagtt
      301 actgtaaaat gtttatacag gtttatgttt atggggtaat tgcacttaat aaatgtatat
      361 tcagaatagc tatgtggctg cgtagctatg ccggcatgtt gttgcctcac caacgctttg
      421 aatacagaag gaggcggatt gagtccttgc tggctgccat tctttataat ggcaataggc
      481 tatatatccg gtgaggtatg agaagaactc ctggcttaat ctaaaatgct attttgtgaa
      541 gatctaacct gcctgattta cctggcgatt ttttagctcg ttaagggtgt atatcctgct
      601 gtactcgcat ttgggtaggt caacgaactc gtacttttgg ttgcgaagct ggggttcaaa
      661 gccagcatcg gcaatggctt tttggatacc gtaggcatcg gtgcggtact tggctccagc
      721 tgacgaaaca acattttcct caatcattat tgacccaagg tcatttgccc cggcatgaag
      781 gcagacctgt gccacttcaa taccggtagt gagccatgag gcctgaatat tttttatatt
      841 gttgagcatt atgcggctaa tggcaatggt gcggatgtac tcctcatcgg taacgtgcgg
      901 gacgataccc tccttctcga gcacggtgcc cttgccctga aatgtccata cgatgaagtt
      961 gacaaacccg ggtgcgttgg ttggcttacg gttttggagg tctctaagct tgataaggtg
//
LOCUS       DAOOQX010000786         1016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_215289_length_1016_cov_2.567118, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000786 DAOOQX010000000
VERSION     DAOOQX010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215289_length_1016_cov_2.567118"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PLX53_10285"
     CDS             <1..379
                     /locus_tag="PLX53_10285"
                     /inference="COORDINATES: protein motif:HMM:NF027664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HOY73513.1"
                     /translation="TRVSCLEGKVKVVAKESKKEAYLTNGMEAKTMGFELSKPDSKKE
                     EFKTSSWTKGEFYYTNTPIGQVFDELERQFDVDLFFDGDTTRTYTGYFKKDNLNSALR
                     LVCIPMDMKWSVNDTLVIINEIK"
     gene            428..>619
                     /locus_tag="PLX53_10290"
     CDS             428..>619
                     /locus_tag="PLX53_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73514.1"
                     /translation="MKRLNVIFAASLMMMAAISFTSCQKDDGDTTDPLVTTMQEGDEV
                     DSYFDETMSEVDELTYESDS"
     assembly_gap    620..629
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            655..>1016
                     /locus_tag="PLX53_10295"
     CDS             655..>1016
                     /locus_tag="PLX53_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73515.1"
                     /translation="MGQGTRTRKTSWEGDIRIDTVTYNNFVNGNARFERVKNGQIIIR
                     TTGHYLGETFIREVSFNNFTINGNLIEGNKRIQKTSQYTYEIQLENGKVTFSDGTTHT
                     RTMTRTRTMVEGYDTPYY"
BASE COUNT          329 a          205 c          231 g          241 t
ORIGIN      
        1 tacccgggtt agctgcctgg aaggtaaagt taaagtggtg gcaaaggaaa gcaaaaagga
       61 agcgtatctt actaatggaa tggaggctaa aactatgggc tttgagctga gtaaacctga
      121 ctccaaaaag gaggaattca aaacctcatc atggactaaa ggtgaatttt actacaccaa
      181 taccccaata gggcaggtgt ttgacgagct tgaacggcaa tttgacgtgg acttgttttt
      241 cgatggtgat accaccagaa cctacaccgg atactttaaa aaggacaacc tgaacagcgc
      301 tcttaggctg gtttgtatcc caatggatat gaagtggagc gttaacgata ctttagttat
      361 aattaatgaa attaagtagc tactaatcat aacaaaaaac ccaatgttta acttaaattt
      421 taatgtaatg aaacgattaa atgtgatttt tgcagcaagc ctcatgatga tggccgcaat
      481 cagctttact tcctgtcaga aggatgatgg ggatacaacc gacccacttg taactaccat
      541 gcaggaaggc gacgaggtgg acagctactt cgacgaaacc atgtccgagg tggatgaatt
      601 aacctacgag agtgactccn nnnnnnnnna atggatatgg ctgtgattaa cagcatggga
      661 caaggaactc gcaccaggaa aacttcgtgg gagggtgata ttaggattga cacggttacc
      721 tataataact ttgtcaatgg caacgccagg tttgagcgcg tgaagaatgg gcagattata
      781 attcgcacca caggccatta tcttggcgaa acctttatcc gtgaggtgtc gttcaacaac
      841 ttcaccatta atggcaacct catcgaaggg aacaagcgaa ttcaaaaaac ttcccagtac
      901 acctacgaga tacagcttga aaacgggaag gtaacttttt ccgacgggac tacccataca
      961 agaaccatga cccgcaccag aactatggta gaaggctatg atactcccta ctacat
//
LOCUS       DAOOQX010000787         1008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_218354_length_1008_cov_2.158447, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000787 DAOOQX010000000
VERSION     DAOOQX010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_218354_length_1008_cov_2.158447"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLX53_10300"
     CDS             complement(<1..388)
                     /locus_tag="PLX53_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73516.1"
                     /translation="MMKRVLFIEPIVAHYRRDTYRLIFSDSRFEFALLAGDSYLDIKP
                     EKVEKTIISTYRKFTLLGEEFYYLRNSLHHVRHFNPDIVVCSGVDFHHLHTLLILLWV
                     KFHPKKKFIWWSHAGLGHQGWWGQMLR"
     gene            complement(394..>1008)
                     /locus_tag="PLX53_10305"
     CDS             complement(394..>1008)
                     /locus_tag="PLX53_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73517.1"
                     /translation="SYSDFPRYSLIPALCSTIGFLLPVVLINRYYLPEQAGFFDLAKL
                     ILSVPMALVATSISSVVLQKVSESYRLGKSFVGELKPLIAFVFIASVVEVLLIVFAGK
                     GIFRVFFGKVWETSGIISKWLVWPFALNFITSSFTSIFVALNRIRWQSLWQVAYFLLI
                     ISLPLFRNFTFYGFIRVYASLEILANLAMVTLLTIVVIKYEKKF"
BASE COUNT          329 a          224 c          208 g          247 t
ORIGIN      
        1 ttcgtagcat ttgtccccac cagccctgat gccccaatcc ggcgtgcgac caccagataa
       61 atttcttttt ggggtgaaat tttacccaga gaagaatgag taaggtatgc agatgatgga
      121 aatcgactcc ggagcaaacc acaatatccg ggttgaagtg tcgcacatga tgcagggagt
      181 tacgtaggta gtagaattcc tcaccgagta gcgtgaactt cctatatgtt gatatgatgg
      241 ttttttcaac cttttcaggt ttaatatcca ggtatgaatc acctgccagc aatgcaaatt
      301 cgaagcggct atcactaaag atcaaccggt aggtatccct tcggtagtgg gcaacaatcg
      361 gctctatgaa aagcacacgc ttcatcatgt cattcaaaat tttttctcgt atttaataac
      421 gacaatagtc agcagcgtaa ccattgccag attggcaagt atttccaaac tagcataaac
      481 cctaataaag ccgtaaaacg tgaaatttct gaatagcgga agtgaaataa tcagcaggaa
      541 gtaggcaact tgccacaagc tttgccacct gattctgtta agcgccacaa aaatagaggt
      601 aaatgatgaa gtaataaagt tcagggcaaa aggccaaacc agccatttcg aaataattcc
      661 cgatgtttcc cacactttcc caaaaaacac cctaaaaatg cccttacccg caaatacaat
      721 cagcagtacc tcaactactg aagctataaa aacaaaagct atcagtggtt taagttctcc
      781 cacaaaggat ttccccagac gatagctttc cgaaactttt tgtagcacta cgctcgaaat
      841 tgaggtggca actagtgcca ttggaaccga taggataagt ttagccagat caaaaaaacc
      901 tgcctgttcg ggcaagtagt acctgttaat cagaactacc ggcaatagga atccaatagt
      961 gctgcatagg gcaggaataa ggctgtaacg gggaaaatcg gaatagct
//
LOCUS       DAOOQX010000788         1007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_218684_length_1007_cov_2.391807, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000788 DAOOQX010000000
VERSION     DAOOQX010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_218684_length_1007_cov_2.391807"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLX53_10310"
     CDS             <1..514
                     /locus_tag="PLX53_10310"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOY73518.1"
                     /translation="ILQTLIEVGPQTLKNPSDYSLRANFMWSCTMALNGLLSTGVTTD
                     WATHMIGHEFTAFFGIDHGATLAAVYPALLTELQEPKHEKIIQYGSRVWGLSGADLTA
                     RNVINKTEDFFRSLGIKTRLCEYGLKQEDVAPIVERFRTRGWKLGERKNINHEVVEAI
                     LKRALEPYRS"
     gene            644..>1007
                     /locus_tag="PLX53_10315"
     CDS             644..>1007
                     /locus_tag="PLX53_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOY73519.1"
                     /translation="MTTHHIHIATLKRFGQLLLLLLLIISCQARQTATYTKVNGFTQG
                     TTFNIIYSDSAGRDFSKAFDSIFHAVDRSMSVYNDSSIISRLNRNEIDTVDALLAEVI
                     AISDSIYHETDGAFDITVG"
BASE COUNT          279 a          243 c          225 g          260 t
ORIGIN      
        1 aattctgcaa accctcatag aagttggccc gcaaaccctg aaaaatccat ccgattactc
       61 ccttcgggct aacttcatgt ggagctgcac catggcccta aacggcttgc tatcaactgg
      121 cgtaacaact gactgggcaa cccacatgat tgggcatgag ttcactgcat tttttggaat
      181 tgaccatggt gccacccttg ccgcagttta cccggcccta cttaccgagc tgcaggaacc
      241 caaacatgaa aaaattatac agtacggcag cagggtatgg gggctgagcg gagcagattt
      301 aaccgcacga aacgtaataa acaaaaccga agattttttc cgttcgctgg ggattaagac
      361 ccgcttgtgc gagtacggat taaagcagga agatgttgct ccaattgttg agaggttccg
      421 cacgcgggga tggaagctgg gtgaaagaaa gaacatcaac catgaagttg tggaggcaat
      481 actcaaacgt gcgcttgaac cctataggtc ttaacctccg ctagatttta taatttagat
      541 tgattggttg aagaaagggt taagcctaat agcatggcct atcagccatt atcccgtacc
      601 taatcatgct gtttgtgcaa tttaaatact tcttacgcaa actatgacta cacaccatat
      661 tcacatagcc acattaaagc gatttggcca gttattgctg ctgttgctgc tgataatttc
      721 ctgccaggca cggcaaacag caacctacac caaggtaaat ggttttacgc agggaacaac
      781 cttcaatatc atttactccg attcggcagg cagggatttc tcaaaagctt ttgactcgat
      841 ttttcatgcg gttgaccgct ccatgtcggt ttacaacgac agctccataa tctcccgtct
      901 aaacagaaac gagattgata cggttgatgc cctgctggcc gaggtaattg ctatctctga
      961 ttctatctac cacgaaaccg atggggcctt cgatattact gttggcc
//
LOCUS       DAOOQX010000789         1001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilaceae bacterium isolate AMR_MDS_2560
            NODE_221036_length_1001_cov_1.679704, whole genome shotgun
            sequence.
ACCESSION   DAOOQX010000789 DAOOQX010000000
VERSION     DAOOQX010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864635
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilaceae bacterium (wastewater metagenome)
  ORGANISM  Tenuifilaceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 12:30:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,065
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,030
            CDSs (with protein)               :: 2,030
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 11 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Tenuifilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221036_length_1001_cov_1.679704"
                     /isolate="AMR_MDS_2560"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2952975"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PLX53_10320"
     CDS             <1..760
                     /locus_tag="PLX53_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOY73520.1"
                     /translation="TVGKANPEASGLWRLSRTLVDVMYNPGNHDNPDWRIVVYAQTPQ
                     AGGFWIGLPNGLTSAKAAAYLGNGIRRTSKIGEYFIRPDAPAMFMSFAEVNFILAEAA
                     QRNLIDISPLTPKDYYEMGIDASYRQYAREIVENIPVYYPSFEGVSINIDDFITDFLN
                     HNGAWDAANPLQKIATQKWVAMFDQGLQAWFEWRRTGYPVLTPAEDGLNGGKIPVRLR
                     YPSDEYSRNGENVAKAVALQGDDNLNTPVWWDCN"
     gene            794..>1001
                     /locus_tag="PLX53_10325"
     CDS             794..>1001
                     /locus_tag="PLX53_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOY73521.1"
                     /translation="MEKFKMYFVKLILPIALTTLSLGSCIENQDLMTSNVRTGGILDA
                     SGLVQFKPSAANQDDSIYLSVYQGP"
BASE COUNT          283 a          237 c          236 g          245 t
ORIGIN      
        1 gaccgttggg aaggccaatc cagaagcctc cggcctgtgg cgtttgagca gaacccttgt
       61 tgatgtcatg tataacccag gtaaccatga taaccccgac tggagaattg tggtttacgc
      121 ccaaacgcca caggccggag gcttctggat tggccttccc aacggtctta catctgctaa
      181 agctgcagca tacctaggca atggaatcag gcgtacttca aaaattgggg agtattttat
      241 tcgacccgac gcacctgcca tgttcatgag cttcgccgaa gtaaacttca tacttgccga
      301 agctgcacaa cgcaacctga ttgacatcag cccacttacc ccaaaggatt actacgaaat
      361 gggaattgat gcttcataca ggcaatatgc cagggaaata gttgaaaata tacccgttta
      421 ctacccatcg tttgaagggg ttagcattaa catagacgat tttattaccg atttcctgaa
      481 ccataacggt gcatgggatg ccgctaaccc actacaaaaa atcgctaccc aaaaatgggt
      541 ggccatgttc gaccagggac ttcaagcttg gtttgaatgg cgaagaactg ggtatccggt
      601 tttaacgcca gcagaggatg gactgaatgg tggtaaaata cccgtaaggc ttcgctaccc
      661 atccgacgag tactcgcgaa atggtgaaaa cgtggcaaaa gcagttgcac tgcagggaga
      721 cgacaaccta aataccccgg tttggtggga ttgcaattaa gctgatttta gaagataaca
      781 atatctaaaa cttatggaaa agttcaaaat gtatttcgtg aagctgattt tgcccattgc
      841 ccttactacc ttgtcgttag gttcttgtat tgaaaaccag gacctcatga ccagcaacgt
      901 gcgtacagga ggtatccttg atgcgtcagg gttagtgcag tttaagccca gtgccgctaa
      961 ccaggacgac tcaatttacc tttcggttta ccagggacca c
//
